LOCUS       DAWHSK010000001        32311 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.001, whole genome shotgun sequence.
ACCESSION   DAWHSK010000001 DAWHSK010000000
VERSION     DAWHSK010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 32311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32311
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.001"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(133..945)
                     /locus_tag="WIC03_000001"
     CDS             complement(133..945)
                     /locus_tag="WIC03_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEZ3882583.1"
                     /translation="MDKIRVAAVQMVSGVSPETNVAAMKRLVARAAEQGVDWVLLPEY
                     WVLMGANDTDKLALAEPLGGGRFQTALSETAKECGVVLFGGTVPLQSPEAGKVMNTLL
                     VYGRDGKRTGLYHKMHLFGFSGLGERYAEADTIRAGGDVPHLSAEGVPVAAGICYDVR
                     FPEFFRRQLPFDVLMLPAAFTHTTGKAHWELLLRARAVENQCYVVAAAQGGLHENGRR
                     TFGHSMIVDPWGDVLDVLPEGEGVVTADIDANRLNSVRNRLPALKYRVLDAV"
     gene            1030..1902
                     /locus_tag="WIC03_000002"
     CDS             1030..1902
                     /locus_tag="WIC03_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEZ3882584.1"
                     /translation="MPILNHIALIGVGLIGGSFVLDLKRQGLVRTVTGIDTDRDNLER
                     ALERGVIDRASVAIDADSIGGADLVLIATPVATVPAILTALRPVLPEHTWISDVGSTK
                     SSVIEAFRRCLPDRLHHCIATHPIAGSDRSGAQAAQFGLFRHRKLIITPHGGEHSDGI
                     ALIENLWHAVGAEIYTMDAQRHDAVFAAVSHMPHLTAFAYVHQILDHPDGQEYLKFAA
                     TGFRDFTRIASGHPAVWADICLANKDSLLQLVQGLGKQLDVLADILTADDREALYRYF
                     EEAKTTRDRWLDGN"
     gene            complement(1959..3113)
                     /locus_tag="WIC03_000003"
     CDS             complement(1959..3113)
                     /locus_tag="WIC03_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HEZ3882585.1"
                     /translation="MKFTKHPVWAMAFRPFYSLAALYGALSVLLWGFGYTGTHELSGF
                     YWHAHEMIWGYAGLVVVAFLLTAVATWTGQPPTRGGVLVGLTIFWLAARIVAFIPGWG
                     ASASGILGTLFFWYGAVCMALPVIRSQNQRNYVAVFALFVLGGTHAAFHVQLHNGNLG
                     GLLSGLQSGLVMVSGFIGLIGTRIISFFTSKRLNVPQIPSPKWVAQASLWLPMLTAML
                     MAHGVMPWLSAAFAFAAGVIFTVQVYRWWYKPVLKEPMLWILFAGYLFTGLGLIAVGA
                     SYFKPSFLNLGVHLIGVGGIGVLTLGMMARTALGHTGNPIYPPPKAVPVAFWLMMAAT
                     AVRMVAVFSSGTAYTHSIRTSSVLFALALLVYAWKYIPWLIRPRSDGRPG"
     gene            complement(3158..3526)
                     /locus_tag="WIC03_000004"
     CDS             complement(3158..3526)
                     /locus_tag="WIC03_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEZ3882586.1"
                     /translation="MKPLKRHPALIGLSRDHHHSLSLCVRLLRTPEERHRDELEPHFS
                     ELETHFREEEAKFAPIWQNVAPELKQRFEKDHARLRQMMASPEYGNAAWNTAFATTLR
                     DHARFEERELFPAAEPFLPA"
     gene            3700..4143
                     /locus_tag="WIC03_000005"
     CDS             3700..4143
                     /locus_tag="WIC03_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEZ3882587.1"
                     /translation="MYLTQHTDYGLRVLIYTAINDDALVNISTIAVTYGISKSHLMKV
                     VTALVKGGFLHSVRGKGGGLRLAAPPDRINIGSVVRHLEPMQLVECMGENNECLITPS
                     CRLTGILGGAMKSFFTYLDGFTLQDLLNKPTYDLLYEPRIPIAVQ"
     gene            complement(4270..4890)
                     /locus_tag="WIC03_000006"
     CDS             complement(4270..4890)
                     /locus_tag="WIC03_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEZ3882588.1"
                     /translation="MAQFFAVHPDNPQERLIKQAVEIVNKGGVVVYPTDSCYALGCKL
                     GDKAAMERILSIRKIDLKHHLTLMCADLSELGTYAKVDNVQFRQLKAATPGPYTFILQ
                     ATKDVPARTLHPKRKTIGLRIPDNAIAQALLGELGEPLLSCTLMLPEDGEPLTDPYEI
                     RERLEHAVDLVIDGGWCGTEPTTVVDMTDGTELVRQGCGDTAVFGL"
     gene            complement(4998..6197)
                     /locus_tag="WIC03_000007"
     CDS             complement(4998..6197)
                     /locus_tag="WIC03_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEZ3882589.1"
                     /translation="MSDQLILVLNCGSSSLKGAVIDRKSGSVVLSCLGERLTTPEAVI
                     TFSKDGNKRQVPLSGRNCHAGAVGMLLNELEKHGLHDRIKAVGHRIAHGGEKYSESVL
                     IDQAVMDELNACIPLAPLHNPANISGILAAQEHFPGLPNVGVMDTSFHQTMPERAYTY
                     AVPRELRKKYAFRRYGFHGTSMRYVAPEAARILGKPLEDIRMIIAHLGNGASITAIKN
                     GKSVDTSMGFTPIEGLVMGTRCGDIDPGVYSYLTSHAGMDVAQVDEMLNKKSGLLGIS
                     ELSNDCRTLEIAADEGHEGARLALEVMTYRLAKYIASMAVGCGGVDALVFTGGIGENS
                     RNIRAKTVSYLDFLGLHIDTKANMEKRYGNSGIISPTDSSPAVLVVPTNEELMIACDT
                     AELAGIL"
     gene            complement(6611..7780)
                     /gene="prpF"
                     /locus_tag="WIC03_000008"
     CDS             complement(6611..7780)
                     /gene="prpF"
                     /locus_tag="WIC03_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylaconitate cis-trans isomerase PrpF"
                     /protein_id="HEZ3882590.1"
                     /translation="MPQIKIPAVYYRGGTSKGIFFKRTDLPEAAQQAGEARDKILLRV
                     LGSPDPYGKQIDGLGNASSSTSKAVILDKSERADHDVDYLFGQVSIDKPFVDWSGNCG
                     NLTAAVGAFSIEQGLVDKGKIPSDGICTVKIWQKNIGKTIIAHVPMQNGAVLETGDFE
                     LDGVTFPAAEVQIEFLDPADGEGSMFPTGNLVDEIDVPNIGRLKATLINAGIPTVFLN
                     AADLGYTGKELQDDINNDAAALEKFEKIRAYGALKMGLISDVSEAAARAHTPKVAFVA
                     PAADYTASSGKTVNAADIDLLVRALSMGKLHHAMMGTASVAIATAAAVPGTLVNLAAG
                     GGTRKEVRFGHPSGTLRVGAAAECQDGQWTATKAVMSRSARVMMEGWVRVPEDCF"
     gene            complement(7944..8390)
                     /locus_tag="WIC03_000009"
     CDS             complement(7944..8390)
                     /locus_tag="WIC03_000009"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3882591.1"
                     /translation="MKTQEDEVTKQKIFNHMINNKSFLNFQFWTFAALSAGSFAFLIS
                     AANIDSKDRDLTLEIAIVGYSVSLILNSILCFLIRGIEDDELSVKMLNEFNLDKRTFW
                     IPILATWSFALSTLFLLFTISLVGAITALIMAVLAIISFIKFTKKS"
     gene            complement(8477..11083)
                     /gene="acnD"
                     /locus_tag="WIC03_000010"
     CDS             complement(8477..11083)
                     /gene="acnD"
                     /locus_tag="WIC03_000010"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe/S-dependent 2-methylisocitrate dehydratase
                     AcnD"
                     /protein_id="HEZ3882592.1"
                     /translation="MAANQRYRKPLPGTDLEYYDARAACEDIKPGSYDKLPYTSRILA
                     ENLVNRADKVDLPTLQSWLGQLIEGKQEIDFPWYPARVVCHDILGQTALVDLAGLRDA
                     IAEKGGDPAKVNPVVQTQLIVDHSLAVECGGYDPDAFRKNREIEDRRNEDRFHFINWT
                     KTAFENVDVIPAGNGIMHQINLEKMSPVVQVKNGVAFPDTCVGTDSHTPHVDALGVIS
                     VGVGGLEAETVMLGRASMMRLPDIVGVELTGKRQPGITATDIVLALTEFLRKERVVGA
                     FVEFFGEGARSLSIGDRATISNMTPEFGATAAMFAIDEQTIDYLKLTGRDDAQVKLVE
                     TYAKTAGLWADALKTAVYPRVLKFDLSSVTRNMAGPSNPHARFATADLAGKGLAKPYE
                     EPSDGQMPDGAVIIAAITSCTNTSNPRNVVAAALLARNANRLGLQRKPWVKSSFAPGS
                     KVAEIYLKEAGLLPEMEKLGFGIVAFACTTCNGMSGALDPKIQKEIIDRDLYATAVLS
                     GNRNFDGRIHPYAKQAFLASPPLVVAYALAGSIRFDIENDVLGVADGKEIRLKDIWPA
                     DEEIDAVVAEYVKPQQFRDVYVPMFDTGTAQKAPSPLYDWRPMSTYIRRPPYWEGALA
                     GERTLRGMRPLAILPDNITTDHLSPSNAILAVSAAGEYLAKMGLPEEDFNSYATHRGD
                     HLTAQRATFANPKLFNEMVKNEDGSVRQGSFARVEPEGETMRMWEAIETYMNRKQPLI
                     IIAGADYGQGSSRDWAAKGVRLAGVEAIVAEGFERIHRTNLIGMGVLPLQFKPGTNRH
                     TLQLDGTETYDVVGERTPRCDLTLVIHRKNGETVEVPVTCRLDTAEEVLVYEAGGVLQ
                     RFAQDFLEGVVA"
     gene            complement(11187..12392)
                     /locus_tag="WIC03_000011"
     CDS             complement(11187..12392)
                     /locus_tag="WIC03_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 domain-containing protein"
                     /protein_id="HEZ3882593.1"
                     /translation="MQTLISADNHLLLWAFVMLAAAAAIFIEQNSRLAGKVPGAVLAL
                     LIALIASNLGVIPTDAPVFDAVWSYIVPLAIPLLLFQLDLHALFKESGRMLFIFLISS
                     LGTVIGTLVSFALLKNYIPELDKVAGMISASYTGGGVNFAAMSAKLNPAKDVIAATIV
                     ADNLMMACYFFILIGISSSYWVRKVWGSPYQDKLEQEYHDPSVNKAAEYWKPKSISLQ
                     SIALSLAASILLVAVSFSLSSFLQNHFQSVDGVALKMLTGLISDKYLLLTTFTLAVVL
                     FFRKGIQKLDGSQELGTYCIYLFFVVIGVPASIPLIIKTAPLLFVFTLIIAVLNLLLT
                     LVLGKLFKFSIEEIVLACNANIGGPTTAAALAISKGWRDLVGAILLIGTVGYIIGNYI
                     GSFVYALLS"
     gene            complement(12418..13140)
                     /locus_tag="WIC03_000012"
     CDS             complement(12418..13140)
                     /locus_tag="WIC03_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEZ3882594.1"
                     /translation="MDKDKGSYAGIPVQNTKELVWQKIQEIPISECGEPLVSVGNTGR
                     IRVNPVYFSQGIEGAINTVLLRRSVADRLTQVVSLLPEMYGLEIFDGWRPVIVQRALR
                     ENMKQEIEKRHPEYDEVQVREALDCFAADPDRAGMPLPHLTGGSVDVGLFDIKSGKSL
                     DMGSEFDATGHLSYSDALEKEGEYFSSARNLRRILIHAMKEAGFSNLPTEWWHFDYGN
                     QNWAYFTQQSNAVFGAVEEQDT"
     gene            complement(13552..14706)
                     /gene="prpC"
                     /locus_tag="WIC03_000013"
     CDS             complement(13552..14706)
                     /gene="prpC"
                     /locus_tag="WIC03_000013"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HEZ3882595.1"
                     /translation="MTETTQTPTFKPKKSVALSGVAAGNTALCTVGRTGNDLSYRGYD
                     ILDLAQKCEFEEVAHLLIHGHLPNKFELAAYKAKLKSMRGLPIRVIKVLESLPAHTHP
                     MDVMRTGVSMLGCVHPERESHPESEARDIADKLIASLGSILLYWYQYSHNGKRIEVES
                     DEETIGGHFLQLLHGKRPSESHIKAMHVSLILYAEHEFNASTFTARVIAGTGSDMYSS
                     ITGAIGALKGPKHGGANEVAYDIQKRYRNADEAEADIRERIGRKEIVIGFGHPVYTIS
                     DPRNVVIKEVARGLSKETGDMRLFDIAERLESVMWEEKKMFPNLDWFSAVSYQKLGVP
                     TAMFTPLFVISRTTGWSAHVLEQRKDGKIIRPSANYTGPEDLAFVEIEDR"
     gene            complement(14791..15666)
                     /gene="prpB"
                     /locus_tag="WIC03_000014"
     CDS             complement(14791..15666)
                     /gene="prpB"
                     /locus_tag="WIC03_000014"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HEZ3882596.1"
                     /translation="MSQHSAGARFRQAVKESNPLAVAGCVNAYFARLATQSGFKAIYL
                     SGGGVAACSCGIPDLGITTMEDVLIDARRITDNVDTPLLVDIDVGWGGAFNIARTIRN
                     FERAGVAAVHIEDQVAQKRCGHRPNKAIVSKDEMVDRIKAAVDARVDENFVIMARTDA
                     LAVEGLDAAIERAQACVEAGADMIFPEAMTDLNMYRQFADAVKVPVLANITEFGSTPL
                     YTQSELAENGVSLVLYPLSSFRAASKAALNVYEAIMRDGTQAAVVDSMQTRAELYEHL
                     NYHAFEQKLDKLFQK"
     gene            complement(16292..17602)
                     /locus_tag="WIC03_000015"
     CDS             complement(16292..17602)
                     /locus_tag="WIC03_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEZ3882597.1"
                     /translation="MDTSKQTLLDGIFKLKANGTTVRTELMAGLTTFLTMCYIVIVNP
                     LILGETGMDMGAVFVATCIASAIGCFVMGFVGNYPIALAPGMGLNAYFTFAVVKGMGV
                     PWQVALGAVFISGLIFILFSFFKVREMLVNALPMGLKMSIAAGIGLFLALISLKGAGI
                     IVANPATLVGLGDIHQPSALLALFGFAMVVVLGHFRVQGAIIITILTITVIASLMGLN
                     EFHGIIGEVPSIAPTFMQMDFEGLFTVSMVSVIFVFFLVDLFDSTGTLVGISHRAGLL
                     VDGKLPRLKRALLADSTAIVAGAALGTSSTTPYVESAAGVSAGGRTGLTAVTVGVLML
                     ACLMFSPLAKSVPAFATAPALLYVGTQMLRSARDIDWDDMTEAAPAFLTIVFMPFTYS
                     IADGIAFGFISYAVVKLLCRRTKDVPPMVWIVAVLWALKFWYLG"
     gene            complement(17741..18919)
                     /gene="ftsZ"
                     /locus_tag="WIC03_000016"
     CDS             complement(17741..18919)
                     /gene="ftsZ"
                     /locus_tag="WIC03_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEZ3882598.1"
                     /translation="MEFVYDVAESAVSPAVIKVIGLGGGGCNAINNMVANNVRGVEFI
                     SANTDAQSLAKNHAAKRIQLGTNLTRGLGAGANPDIGRAAAQEDREAIEEAIRGANML
                     FITTGMGGGTGTGSAPVVAEIAKSLGILTVAVVTRPFAYEGKRVHVAQAGLEQLKEHV
                     DSLIIIPNDKLMTALGEDVTMREAFRAADNVLRDAVAGISEVVTCPSEIINLDFADVK
                     TVMSNRGIAMMGSGYAQGIDRARMATDQAISSPLLDDVTLDGARGVLVNITTAPGCLK
                     MSELSEVMKIVNQSAHPDLECKFGAAEDETMSEDAIRITIIATGLKEKGAVDFVPARE
                     VEAVAPSKQEQSHNVEGMIRTNRGIRTMNLTAADFDNQSVLDDFEIPAILRRQHNSDK
                     "
     gene            complement(19038..20282)
                     /gene="ftsA"
                     /locus_tag="WIC03_000017"
     CDS             complement(19038..20282)
                     /gene="ftsA"
                     /locus_tag="WIC03_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEZ3882599.1"
                     /translation="MEQQQRYISVLDIGTSKVLALIGEVQDDDKINIVGLGQAPSRGL
                     RAGMVTNIDATVQAIRQAVNDAELMADTKITHVTTGIAGNHIRSLNSQGVVKIKDGEV
                     TQADIDRAIETAKAINIPPDQKILDAVVQDYIIDTQLGVREPIGMSGVRLDTRVHIIT
                     GASTAVQNVQKCIERCGLKSDQIMLQPLASGQAVLTEDEKDLGVCVIDIGGGTTDIAV
                     YMNGAIRHTSVIPAGGNLITKDLSKSLRTPLDAAEYIKIHYGVASCDTEGLGEMIEVP
                     GVGDRTSRQVSSKVLAAIISARIQEIFGVVLGELQKSGFPKEVLNAGIVLTGGVSMMT
                     GIVEFAEKIFDLPVRTGAPQEMGGLSDRVRTPRFSTAIGLLHAACKLEGNLPQPENGA
                     VQEREVGGGLLARLKRWIENSF"
     gene            complement(20368..21096)
                     /locus_tag="WIC03_000018"
     CDS             complement(20368..21096)
                     /locus_tag="WIC03_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEZ3882600.1"
                     /translation="MWDNAEAMERLTRWLLVMMAMLLAASGLVWFYNSNHLPVKQVSL
                     KGNLVYSDKKALGSLAKEYIHGNILRTDINGAQEAYRRYPWIASVMVRRRFPDTVEVV
                     LTERKPVARWGDHALVDGEGNVFEARLDRPGMPVFRGAEGTSAEMLRRYDEFSTVLAK
                     QGLGIKEMTYTARSAWIVVLDNGITVRLGRENEMKRLRLFTEAWQHLLRKNKNRLSYV
                     DMRYKDGFSVRYASDGLPEKESEE"
     gene            complement(21086..22000)
                     /locus_tag="WIC03_000019"
     CDS             complement(21086..22000)
                     /locus_tag="WIC03_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEZ3882601.1"
                     /translation="MQNFGKVAVLMGGFSSEREISLDSGTAILNALKSKGIDAYAFDP
                     KETPLSELKAQGFQTAFNILHGTYGEDGAVQGALELLGIPYTGSGVAASAIGMDKYRC
                     KLIWQALGLPVPEFAVLHDDTDFDAVEEKLGLPMFVKPAAEGSSVGVVKVKGKGRLKS
                     VYEELKHLQGEIIAERFIGGGEYSCPVLNGKGLPGIHIIPATEFYDYEAKYNRDDTIY
                     QCPSEDLTEAEESLMRELAVRGAQAIGAEGCVRVDFLKDTDGKLYLLEINTLPGMTSH
                     SLVPKSAAVTGVGFADLCIEILKTAHVG"
     gene            complement(22114..23523)
                     /locus_tag="WIC03_000020"
     CDS             complement(22114..23523)
                     /locus_tag="WIC03_000020"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEZ3882602.1"
                     /translation="MMKNRVTNIHFVGIGGVGMSGIAEVLHNLGFKVSGSDQARNAAT
                     EHLGSLGIQVYPGHTAEHVNGADVVVTSTAVKKENPEVVAALEQQIPVIPRALMLAEL
                     MRFRDGIAIAGTHGKTTTTSLTASILGAAGLDPTFVIGGKLNAAGTNARLGKGEYIVA
                     EADESDASFLHLTPIMSVVTNIDEDHMDTYGHSVEKLHQAFIDFIHRMPFYGKAFLCI
                     DSEHVRAILPKVSKPYATYGLDDTADIYATDIENVGAQMKFTVHVQMKGHEQGSFEVV
                     LNMPGRHNVLNALAAIGVALEVGASVEAIQKGLLGFEGVGRRFQKYGDIKLPNGGTAL
                     LVDDYGHHPVEMAATLSAARGAYPEKRLVLAFQPHRYTRTRDLFEDFTKVLNTVDALV
                     LTEVYAAGEEPIAAADSRALARAIRVLGKLEPIYCENVADLPEMLLNVLQDGDIVLNM
                     GAGSINRVPAALLELSKQI"
     gene            complement(23813..24880)
                     /gene="murG"
                     /locus_tag="WIC03_000021"
     CDS             complement(23813..24880)
                     /gene="murG"
                     /locus_tag="WIC03_000021"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEZ3882603.1"
                     /translation="MGGKTFMLMAGGTGGHIFPALAVADSLRARGHHVIWLGSKDSME
                     ERIVPQYGIRLETLAIKGVRGNGIKRKLMLPVTLYQTVREAQRIIRKHRVECVIGFGG
                     FVTFPGGLAAKLLGVPIVIHEQNAVAGLSNRHLSRWAKRVLYAFPKAFSHEGGLVGNP
                     VRADIAKLPAPAERFQGREGRLKILVVGGSLGADVLNKTVPQALALLPDNARPQMYHQ
                     SGRGKLGSLQADYDALGVKAECVEFITDMVSAYRDADLVICRAGALTIAELTAAGLGA
                     LLVPYPHAVDDHQTANARFMVQAEAGLLLPQTQLTAEKLAEILGSLNREKCLKWAENA
                     RTLALPHSADDVAEAAIECTA"
     gene            complement(24884..26209)
                     /locus_tag="WIC03_000022"
     CDS             complement(24884..26209)
                     /locus_tag="WIC03_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HEZ3882604.1"
                     /translation="MQTDWQVVFLKISEVLVKVGDGVHTLLLDRPIVRDGRKFDAPLL
                     WMVVLMTAFSLLMIYSASVYLASKEGGDQFFYLTRQAGFVVAGLIASGLLWFLCRMRT
                     WRRLVPWIFALSGLLLVVVLIAGREINGATRWIPLGPLNFQPTELFKLAVILYLASLF
                     TRREEVLRSMESLGWQSIWRGTANLIMSATNPQARRETLEMYGRFRAIILPIMLVAFG
                     LVLIMVQPDFGSFVVITVIAVGMLFLAGLPWKYFFVLVGSVLGGMVLMITAAPYRVQR
                     VVAFLDPWKDPQGAGYQLTHSLMAIGRGEWFGMGLGASLSKRGFLPEAHTDFIFAIIA
                     EEFGFFGMCVLIFCYGWLVVRAFSIGKQSRDLGLTFNAYIASGIGIWIGIQSFFNIGV
                     NIGALPTKGLTLPLMSYGGSSVFFMLISMMLLLRIDYENRRKMRGYRVE"
     gene            complement(26335..27672)
                     /locus_tag="WIC03_000023"
     CDS             complement(26335..27672)
                     /locus_tag="WIC03_000023"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEZ3882605.1"
                     /translation="MTFQNKKILVAGLGGTGISMIAYLRKNGAEVAAYDAELKPERVS
                     QIGKMFDGLVFYTGRLKDALDNGFDILALSPGISERQPDIEAFKQNGGRVLGDIELLA
                     DIVNRRGDKVIAITGSNGKTTVTSLVGYLCIKCGLDTVIAGNIGTPVLEAEWQREGKK
                     ADVWVLELSSFQLENTESLRPTAATVLNISEDHLDRYDDLLDYAHTKAKIFRGDGVQV
                     LNADDAFCRAMKRAGREVKWFSLEHEADFWLERETGRLKQGNEDLIATKDIPLQGLHN
                     AANVMAAVALCEAVGLPREALLEHVKTFQGLPHRVEKIGEKNGVVFIDDSKGTNVGAT
                     AAAIAGLQNPLFVILGGMGKGQDFTPLRDALAGKAKGVFLIGVDAPQIRRDLDGCGLN
                     MTDCATLEEAVQTAYAQAEACDIVLLSPACASFDMFKGYAHRSEVFIEAFKAL"
     gene            complement(27760..28140)
                     /locus_tag="WIC03_000024"
     CDS             complement(27760..28140)
                     /locus_tag="WIC03_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HEZ3882606.1"
                     /translation="MKQTVKWLAAALIALGLNQAVWADDVSDFRENLQAAEQGNAAAQ
                     YNLGAMYYKGQGVRRDDAEAVRWYRQAAEQGLAQAQNNLGVMYAERRGVRQDRALAQE
                     WLGKACQNGNQDGCDNDQRLKAGY"
     gene            complement(28266..29348)
                     /locus_tag="WIC03_000025"
     CDS             complement(28266..29348)
                     /locus_tag="WIC03_000025"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEZ3882607.1"
                     /translation="MFLWLAHFSNWLTGLNIFQYTTFRAVMAALTALAFSLMFGPWTI
                     RRLTALKCGQAVRTDGPQTHLVKNGTPTMGGSLILTAITVSTLLWGNWANPYIWILLG
                     VLLATGALGFYDDWRKVVYKDPNGVSAKFKMVWQSSVAIIAGLALFYLAANSANNILI
                     VPFFKQIALPLGVVGFLVLSYLTIVGTSNAVNLTDGLDGLAAFPVVLVAAGLAIFAYV
                     SGHYQFSQYLQLPYVAGANEVAIFCTAMCGACLGFLWFNAYPAQVFMGDVGALALGAA
                     LGTVAVIVRQEFVLVIMGGLFVVEAVSVMLQVGWYKKTKKRIFLMAPIHHHYEQKGWK
                     ETQVVVRFWIITIVLVLIGLSTLKIR"
     gene            complement(29442..29618)
                     /locus_tag="WIC03_000026"
     CDS             complement(29442..29618)
                     /locus_tag="WIC03_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3882608.1"
                     /translation="MKSRRFFKALLLIAALVGAFYAGMQTQAYLYEDLCLDLGGGKNP
                     GSYPICVIGKVSAR"
     gene            complement(29621..30979)
                     /locus_tag="WIC03_000027"
     CDS             complement(29621..30979)
                     /locus_tag="WIC03_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEZ3882609.1"
                     /translation="MKPLDLNFICQALKLPMPSESKPVSRIVTDSRDIRAGDVFFALA
                     GGRFDAHDFVEDVLAAGAAAVVVSREDCVAMDGALKVDDTLTALQMVAKAWRENVNPF
                     VFGITGSGGKTTVKEMLAAVLRRRFGDNAVLATAGNFNNHIGLPLTLLKLNEKHRYAV
                     IEMGMNHFGELAVLTQIAKPDAALINNAMRAHVGCGFDGVGDIAKAKSEIYQGLCSDG
                     MALIPQEDANMAVFKTATLNLNTRTFGIDSGDVHAENIVLKPLSCEFDLVCGDERAAV
                     VLPVPGRHNVHNAAAAAALSLAAGLSLNDVAEGLKGFSNIKGRLNVKAGIKGATLIDD
                     TYNANPDSMKAAIDVLARMPAPRIFVMGDMGELGEDEAAAMHAEVGAYARDQGIEAAY
                     FVGDNSVEAAEKFGADGLWFAAKDPLIQVLRHDLPERATVLVKGSRFMQMEEVVEALE
                     DK"
     gene            complement(31103..32266)
                     /locus_tag="WIC03_000028"
     CDS             complement(31103..32266)
                     /locus_tag="WIC03_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HEZ3882610.1"
                     /translation="MTLILSCAVMDKIASDKDLREPDAGLLLNIFDLYYDLASAPAQY
                     VAKRAHILEAAKKASTWHIRHVAPKYKNYVVVIGESARSDYMNVYGFPLPDTPFLSQT
                     KGLLINGYQSTAHATNLSLPQTLGLPGEPNNNIVSLAKQAGFRTAWLSNQGMLGHFAN
                     EISTYALRSDYPWFTQRGDYGKSAGLSDRLLLPAFKRVLIGNAGTKPRLIVMHLMGSH
                     SDFCTRLDKDARRFQYQTEKISCYVSTIAQTDKFLEDTVKILNENKESWSLVYFSDHG
                     LMHVGKGGERTLTHGAWKRQSYGVPLVKISSDDTRREMIKVRRSAFNFLRGFGSWTGI
                     ETDELPDDGYDFWGNVPDVQGEGNNLAFIDGLPDDPAPWYAGKGKSTKNTSKK"
BASE COUNT         7770 a         8898 c         8051 g         7592 t
ORIGIN      
        1 ggactggggc gtcgaaaggg gcggattcga tggattcgat gaaaacggta gaaatgtggg
       61 attgatggaa atggcaggct gaagcccatc ctacagcccg ctgattcccc ggcaccgatg
      121 ccgtctgaac cttcagacgg catccaaaac ccggtatttc aaggcgggca ggcggttgcg
      181 gacgctgttc aggcggttgg catcgatgtc tgccgtaaca acgccttcgc cttcgggcaa
      241 tacgtccaac acgtcgcccc acggatcgac aatcatgctg tgtccgaacg tgcgccgtcc
      301 gttttcgtgc aaaccgccct gtgccgccgc cacgacgtaa cattggtttt cgacggcacg
      361 cgcgcgcagc agcagctccc aatgcgcctt gcccgtcgtg tgcgtaaacg cagcgggcag
      421 catcaatacg tcaaacggca gctggcggcg gaaaaattcg ggaaagcgga catcgtaaca
      481 aatacctgcc gccaccggca cgccttctgc cgacaagtgc ggcacatccc cgcccgcgcg
      541 gatggtatcg gcttcggcat agcgttcgcc caaaccggaa aagccgaaga ggtgcatttt
      601 gtggtacagc cccgtccgtt tgccgtcgcg cccgtacacc aataaagtat tcatgacctt
      661 gcccgcctcg gggctttgca gcggcacggt tccgccgaac agcaccacgc cgcattcttt
      721 cgccgtttcg ctcaatgccg tctgaaagcg tccgccgccc aaaggctcgg caagcgcgag
      781 tttgtcggta tcgtttgcgc ccatcagcac ccaatattcg ggcagcagca cccaatccac
      841 accctgctcc gccgcccgtg cgaccaggcg tttcatggca gcgacgttgg tttccggcga
      901 cacgcccgac accatctgca cggcggcaac tctgattttg tccatttctt ctcctttttc
      961 ctgccgacct tgcggcatat tccgccgata ggctaccatg acggtacttt gcgaaaaccg
     1021 tttacacaaa tgcccatttt gaaccatatt gccctcatcg gtgtagggct gatcggcggt
     1081 tcgttcgttc tcgacctcaa aaggcaggga ctcgtccgca ccgttaccgg tatcgacacc
     1141 gaccgcgaca acctcgaacg tgcattggaa cgcggcgtga ttgaccgggc ttccgttgcc
     1201 atcgacgcgg acagcatcgg cggtgcggac ttggtactga ttgccacgcc cgtcgccacc
     1261 gttcctgcca ttttgaccgc gctgcgcccc gttttgccgg aacacacttg gatttccgat
     1321 gtcggcagca ccaaatcttc ggtcatcgaa gccttccgcc gctgtctgcc cgaccgcctg
     1381 caccactgca tcgccaccca cccgattgcc ggttcggaca gaagcggtgc gcaagccgcg
     1441 cagttcgggc tgttccgcca cagaaaactc atcatcacgc cacacggcgg cgaacattca
     1501 gacggcattg ccttgataga aaacctgtgg cacgcggtcg gtgcggaaat ttatacgatg
     1561 gacgcgcaac gccacgacgc ggttttcgcc gccgtctccc atatgcccca cctgaccgcc
     1621 ttcgcctatg tccaccagat tctcgaccac cccgacggac aggaatatct gaaattcgcc
     1681 gccacgggct ttcgggactt cacccgcatc gcctccggcc atcccgccgt gtgggcggac
     1741 atctgccttg ccaacaaaga cagcctgctg caactggttc aaggcttggg caaacagttg
     1801 gacgttttag cagacatcct gaccgccgac gaccgcgaag ccctgtaccg ctattttgaa
     1861 gaagccaaaa ccacacgcga ccgctggctg gacggcaact gaccgccctg ccgtctgaaa
     1921 agcaaaccgc ccagacccga aatctgcggc ggtttgtctc aaccgggcct gccgtccgaa
     1981 cgcggacgaa tcagccaagg aatatacttc cacgcataca ccaaaagcgc gagtgcaaac
     2041 aaaaccgaag aggtgcgtat gctgtgcgtg taggcagtgc cggaagaaaa tacggcaacc
     2101 atacggacgg cggttgccgc catcatcagc caaaacgcaa cgggaacggc tttgggcggc
     2161 ggataaatcg gattgcccgt atgaccaagc gcggtacgcg ccatcatgcc caaagtcagc
     2221 acgccgatac cgccgacccc gatcagatgc acacccagat tgaggaaaga gggtttgaaa
     2281 taagacgcgc cgaccgcaat cagccccaat ccggtaaaca gatagccggc aaacagaatc
     2341 cacagcatcg gctctttcaa cacaggctta taccaccagc ggtacacctg cacggtaaaa
     2401 atcacacctg ccgcaaacgc gaaagccgcc gacagccaag gcatcacgcc gtgcgccatc
     2461 agcatggcgg tcagcatggg cagccacagc gaagcctgcg ccacccattt cggactggga
     2521 atctgcggca cattcaaccg tttggacgta aaaaacgaaa taatccgcgt accaatcaga
     2581 ccgataaaac ccgacaccat caccaagccc gactgcaatc cgctcaagag tccgcctaag
     2641 ttgccgttgt gcagctggac gtggaacgcc gcgtgcgtac cgcccaagac gaacagcgcg
     2701 aacacggcaa catagttgcg ttgattctgc gaacggataa cgggcaaagc catgcacacc
     2761 gcgccgtacc agaaaaacag cgtaccgagt atgccgcttg ccgacgcacc ccaacccggg
     2821 ataaaggcga caatccgcgc agccagccaa aagatagtca agccgaccag aacgccgccc
     2881 cgcgtgggcg gctgccccgt ccaagtggcg acggcggtca gcaggaaggc gacgacgacc
     2941 agtccggcat aaccccaaat catctcatgc gcgtgccaat agaaaccgga cagctcatgc
     3001 gttcccgtgt agccgaaacc ccacagcaat acggacaatg cgccgtacag agccgccagc
     3061 gaataaaatg ggcggaacgc cattgcccag acggggtgct tggtaaattt catgtgtgtt
     3121 ccttgtttat cattaatata tttgccgcaa aacggaatca tgccggcaaa aacggttcgg
     3181 cggcgggaaa cagctcgcgt tcttcaaagc gcgcgtggtc gcgcagggtt gtggcaaaag
     3241 cggtattcca cgccgcgtta ccgtattcgg ggcttgccat catctgccgc agtcgggcgt
     3301 ggtctttctc gaaacgttgt ttcaattcgg gggcgacatt ctgccaaatt ggggcaaact
     3361 tggcttcttc ttcgcgaaaa tgggtttcca attcggaaaa atgcggttcg agttcgtccc
     3421 gatgcctttc ttccggcgtc cgcaacagac gcacgcacag ggaaagcgaa tggtggtggt
     3481 cacgcgaaag cccgataagg gcgggatgtc gtttcaacgg tttcatgatt ttgagttaca
     3541 ataacaaatt ctcaaacggt agcgcggatg tttcagacgg cataccgccg cagacaggct
     3601 gcattctacc gccccgacgg acagggtttc aaccattaag gtattgtccg tgaaccttaa
     3661 accgtaccga taagaataac aatccatacc gagaaaatca tgtatttgac acaacatacg
     3721 gactacgggc tgcgcgtcct tatctacact gccatcaacg acgatgcgct ggtcaacatc
     3781 agtaccatcg ccgtaaccta cggcatttcc aaaagccatc tgatgaaggt cgttaccgcg
     3841 ctggtcaaag gcggcttcct ccacagtgtg cgcggaaaag gcggcggtct gcggcttgcc
     3901 gcaccgcccg accgcatcaa catcggctcg gttgtccgcc acctcgaacc gatgcagctg
     3961 gtcgagtgca tgggcgagaa caacgaatgc ctgattacac cgtcctgccg gctgacgggc
     4021 atactcggcg gcgcgatgaa gtcgtttttc acgtatctgg acggtttcac gctccaagac
     4081 ctgctcaaca agccgaccta cgacctgctc tacgaaccga gaattccgat tgcggtgcag
     4141 taatgcatca aaatgccgtt tcaaacggat attccgaccg agcgcgatac gcgccggcat
     4201 ttcggactgc cgccggcaag gcgcgcaatg ccgtctgaac agccgatgct tcagacggca
     4261 tcggtttccc tacaaaccga acaccgccgt atcgccgcaa ccttggcgca ccaattccgt
     4321 gccgtcggtc atatcgacga cggtggtcgg ctcggttccg caccagccgc cgtcaatcac
     4381 caaatcgacg gcgtgttcca aacgctcgcg gatttcataa ggatcggtca atggttcgcc
     4441 gtcttcgggc agcatcaggg tgcagcttaa aagcggctcg cccaattccc ccagcagggc
     4501 ttgtgcaatg gcattatcgg gaatacgcag cccgatggtt ttgcgtttcg ggtgcagcgt
     4561 gcgcgccggc acatccttcg tcgcctgtaa aataaaagta taaggcccgg gtgtggcggc
     4621 tttaagctga cgaaactgta cgttgtcgac tttggcgtat gtgcccaact cgctcaaatc
     4681 tgcgcacatc agggtcaggt ggtgtttcaa atcgattttg cggatggaga gtatgcgttc
     4741 catcgccgcc ttatcgccga gtttgcagcc caaggcataa caggaatcgg tcggataaac
     4801 gaccacgccg cctttattga cgatttcgac cgcctgcttg atgaggcgtt cttgggggtt
     4861 gtcgggatga acggcgaaaa actgtgccat agtttgtgtc cttatcgtca tgttttccgt
     4921 attgtacgcc caaaatccaa acaaaatgcc gtctgaaaat ttcagacggc atttttgcgg
     4981 actcgtccct tttttggcta caagatgccg gcaagttcgg cagtgtcgca ggcaatcatc
     5041 agttcttcat tggtcgggac aaccaaaaca gccggagaag aatcggtcgg gctgataatg
     5101 cccgaattgc cgtagcgttt ttccatattg gctttggtgt cgatgtgcag acccaagaaa
     5161 tcaagatagg aaacggtttt ggcacggata ttacgcgagt tttcgccgat accgccggtg
     5221 aacacgagtg cgtcaacgcc gccgcagccc acagccatcg aagcgatgta tttggcgagg
     5281 cggtaggtca tgacttcgag ggcgaggcgc gcgccttcgt ggccttcgtc ggcggcgatt
     5341 tcgagggtgc ggcagtcgtt ggaaagttcg gaaataccga gcaaacctga ttttttgttc
     5401 agcatttcat ccacttgggc aacatccatc ccggcgtggg aagtcagata gctgtatacg
     5461 cccggatcga tgtcgccgca acgtgtaccc attaccaaac cttcgatcgg cgtgaaaccc
     5521 atactggtat cgacggattt gccgtttttg atggcggtaa tggatgcgcc gttgcctaag
     5581 tgggcaataa tcatgcggat gtcttccaga ggtttgccca agatgcgtgc ggcttcaggg
     5641 gcaacgtaac gcatactggt gccgtggaaa ccgtagcggc ggaaagcgta ttttttacgc
     5701 aactcgcgcg gcacggcata agtgtaggca cgctccggca tggtttggtg gaacgaagta
     5761 tccatcacgc cgacattggg cagaccgggg aaatgttcct gtgcggcaag gatgccgctg
     5821 atgttggcgg ggttgtgcag cggcgcaagc ggaatgcagg cattgagttc gtccattacg
     5881 gcctggtcga tcaaaacaga ctcgctgtat ttttcgccgc cgtgggcgat gcggtggccg
     5941 acggctttga tgcggtcgtg cagtccgtgt ttttccagtt cgttcagcag catacccacc
     6001 gcgccggcat ggcagttgcg gccgctcagg ggaacttggc gtttgttgcc gtctttgctg
     6061 aacgtaatga cggcttcggg cgtggtcagg cgttcgccga ggcagcttag gacgacgctg
     6121 ccgcttttgc ggtcgataac ggcacctttg agcgatgaac tgccgcagtt cagaacaaga
     6181 atgagttggt cggacatgat gtttctcctt gtagtttgtg gttgtttttt ggtgtggggt
     6241 cgtctgaaaa aggatgggcg ttgggcggag tgtgtttcag acggcctctt tgggcaaagc
     6301 atatgccgca ttttctatcg ttattccgtc cccgcctacg cgaggacagg ctgtggcggg
     6361 aatccagaat gtagaactca cgaaacctgt tttcccctgc caatcctcca tattgacagg
     6421 tctggattcc cgcctgcgcg ggaatgacgg taatacggga agctgtcttt tatcggggtt
     6481 tccgtgttct tccttgtgat ttgccgcctt ccgccacagg gcaagggaaa ggaatggtca
     6541 gtgatttgat gtttacttgt ttttcagacg acctttttat ggctcgcggg caaacccatg
     6601 ctacgtcaat ttaaaaacaa tcctcaggca ccctgaccca accttccatc atcacgcgtg
     6661 cgctacggct catgaccgct ttggtggccg tccattgtcc gtcctgacat tcggcggctg
     6721 caccgacgcg caatgtgccg gaaggatgcc cgaagcgcac ttctttacgc gttccgccgc
     6781 ctgcggcaag gttgaccagc gtaccgggta cggcggcggc ggtcgcaatg gcaacagagg
     6841 cggtacccat catcgcgtgg tgcagtttgc ccatgctcag ggcgcgtacc agcaaatcga
     6901 tgtcggcggc gttcacggtt ttgccactgg aggcggtgta atcggcggcg ggcgcgacga
     6961 aggcgacttt cggcgtgtgc gcgcgagcgg cagcttcgga tacgtcgctg atcagaccca
     7021 ttttcagcgc accgtaagcg cggattttct cgaatttttc caaagccgcg gcatcgttgt
     7081 tgatgtcgtc ttgcaactct ttgcctgtgt agcccaagtc ggcggcattc aagaaaacgg
     7141 tcggaatgcc cgcgttgatg agcgtggctt tcaaacggcc tatattcggc acatcaattt
     7201 catcgaccaa attgccggtt gggaacatac tgccttcgcc gtcggctgga tcaagaaatt
     7261 cgatttgtac ttcggctgcc gggaacgtta cgccgtcgag ctcaaaatcg cctgtttcca
     7321 aaactgcgcc gttttgcatc ggtacatggg caataatggt tttgccgatg tttttctgcc
     7381 agattttgac cgtgcagatg ccgtctgaag gaatcttgcc tttatcgacc aagccctgtt
     7441 cgatggagaa tgcgcccacg gcagcggtga ggttgccgca gttgccgctc caatcgacaa
     7501 aaggtttgtc gatggaaact tgcccgaaaa ggtaatcgac atcgtgatcg gcgcgttcgg
     7561 acttgtccaa aatcacggct ttgctggtgg acgaactggc gttgcccaaa ccgtctatct
     7621 gcttgccgta gggatccggg ctgccgagta cgcgcaacag gattttgtcg cgtgcctcgc
     7681 ctgcttgttg cgccgcttcg ggcaggtcgg tgcgtttgaa gaaaatgcct ttagatgtac
     7741 cgccgcggta gtaaacggcg gggattttaa tttgcggcat ttcgagttct cctttgtatg
     7801 atgcagatta gcagcagagg taggttgggt tgcaaacccg aaaaaaccgt tggatttagc
     7861 aaaatgccgg ggcttaactc aacctacaac ttctgtgtcg ggcatcaatg cccgatttac
     7921 gcaccacttc aaattgtaaa aatctatgat ttttttgtaa attttataaa actaattatt
     7981 gctaaaacag ccattataag agcagtaatt gctcctacta gactaatggt aaacaataag
     8041 aaaagtgtcg aaagcgcaaa actccacgtt gctagaatgg gaatccaaaa agtacgctta
     8101 tccaaattaa attcatttaa cattttaacc gataactcat cgtcttctat gcctcttatt
     8161 aaaaaacata gtatggaatt taatattaaa cttactgaat atccaacaat ggctatttca
     8221 agcgtcaagt ctctatcttt agaatctata tttgctgcac ttattagaaa ggcaaatgaa
     8281 cctgcactca aagcagcaaa tgtccaaaac tggaaattta aaaatgattt attattaatc
     8341 atatgattaa aaattttctg cttagttacc tcatcttctt gtgtcttcat atttactgtt
     8401 taatttaaat taaaaattta accataaata attttcaaac ggtcttctca taaatcataa
     8461 aaaagaccgt ctacctttat gctaccacac cttctaaaaa gtcttgtgca aaccgttgca
     8521 gcacgccgcc ggcttcgtac accagcactt cctctgcggt atcgaggcgg caggtaacgg
     8581 gaacttcaac ggtttcgccg tttttacggt gaatcacgag ggtcaggtcg cagcgcggtg
     8641 tacgttcgcc gaccacgtcg taggtttccg taccgtccag ttgcagggta tggcggttgg
     8701 tacccggttt gaactgcaac ggcaacacgc ccataccgat caagttggtg cggtggatac
     8761 gctcgaagcc ttcggcaaca atcgcttcca cgccggcgag gcgtacgcct tttgcagccc
     8821 agtcgcggct tgagccttga ccgtagtccg caccggcaat gatgatgagc ggctgtttgc
     8881 ggttcatata ggtttcgatg gcttcccaca tgcgcatggt ttcgccttcg ggttcgacgc
     8941 gagcgaacga gccttggcgc acgctgccgt cttcgttttt caccatttcg ttaaacagtt
     9001 tcggattggc gaaggtagcg cgttgggcgg tcaagtggtc gccgcggtgg gttgcgtaag
     9061 agttgaagtc ttcttcaggc aaacccattt tcgccaaata ctcgcctgcg gcactgacgg
     9121 ccaaaatcgc attggacggc gagaggtggt cggtggtgat gttgtcgggc aaaatcgcca
     9181 gcggacgcat acctcttaat gtgcgttccc ctgccagcgc gccttcccag taaggcggac
     9241 ggcggatgta ggtggacatc ggacgccaat cgtacagcgg actgggtgct ttttgcgctg
     9301 tgccggtgtc gaacatcggt acatacacat cgcggaactg ctgcggtttc acatattcgg
     9361 caacgacggc atcgatttct tcatcggcag gccaaatgtc tttcaggcgg atttccttgc
     9421 cgtctgcaac gccgagtacg tcgttttcaa tatcgaaacg gatactgcct gccagcgcgt
     9481 aggcaacgac caacggaggc gaagcgagga aagcctgttt cgcatacggg tggatacggc
     9541 cgtcgaagtt gcggttgcct gataatacgg cggtggcgta caaatcgcgg tcgatgattt
     9601 ctttttggat tttcggatcc agcgcaccgc tcatgccgtt acaggtggta catgcgaagg
     9661 caacgatacc gaagccgagt ttttccattt caggcagtag gcctgcttct ttcaaataga
     9721 tttcggctac ttttgaaccc ggggcaaacg aagatttcac ccaaggttta cgttgcaagc
     9781 cgaggcggtt ggcattgcgt gccaacagcg cggcggcgac aacgttgcgc ggattggaag
     9841 tattggtaca ggaagtaatc gcggcaataa tcactgcacc gtcaggcatt tggccgtctg
     9901 aaggctcttc ataaggttta gccaagcctt tgccggccaa atcggcagtc gcaaaacggg
     9961 catgcgggtt acttgggcct gccatgttgc gcgttacgct gcttaaatca aatttcaata
    10021 cgcgaggata aacggcggtt ttcaaggcat ctgcccacaa gcctgcggtt ttggcgtagg
    10081 tttccaccaa tttcacttgc gcgtcgtcgc ggcctgtcag tttcaaataa tcaatggttt
    10141 gctcatcaat agcgaacatc gcggcagtcg cgccgaactc aggggtcatg ttggaaatag
    10201 tggctcggtc gccgatagac aggcttcttg cgccctcgcc gaagaattcg acaaatgcgc
    10261 caaccacgcg ctctttacgc aagaattcgg tcagtgccaa cacgatgtcg gtggcagtaa
    10321 tgccaggttg acgtttgcca gtcaactcaa cgccgacaat atcaggcaga cgcatcatgg
    10381 atgcacggcc taacattacg gtttcggctt ccaaaccgcc cacgcctacg gaaatcacgc
    10441 ccagcgcatc gacgtgtggc gtgtgcgaat ccgtgccgac gcaggtatcg gggaaagcca
    10501 cgccgttttt gacttggacg acgggcgaca ttttttccag attgatttgg tgcataatgc
    10561 cgttgcccgc cggaatcacg tccacatttt caaatgcggt ttttgtccag ttgatgaagt
    10621 ggaaacggtc ttcgttgcgc cggtcttcga tttcgcggtt tttgcggaag gcatcaggat
    10681 cgtaaccgcc gcactccacc gccagagagt ggtcgacgat aagctgggtt tgcaccaccg
    10741 gattcacttt ggcaggatcg ccgccttttt cggcaatcgc atcgcgcaag cccgccaaat
    10801 ccaccagcgc ggtctgcccc agaatatcgt ggcacaccac ccgcgcggga taccacggaa
    10861 agtcgatttc ctgcttccct tctatcaact ggcccagcca gctttgcagc gtcggcaggt
    10921 cgactttgtc tgcgcggttg accaagttct cagccaagat acggcttgta taaggcagct
    10981 tgtcgtaaga gccgggtttg atgtcctcac acgccgcacg cgcgtcgtag tattccaaat
    11041 ccgtaccggg tagcggtttg cggtaacgtt ggttggcagc catgttcagt tctcctgtgg
    11101 atctattttt cttgtggttt ggggtttcag acgacgtttt ttgaaggggg tcgtctgaaa
    11161 aggttcatgg attgttagat tagtcttcag ctcaataggg catatacaaa cgaacctata
    11221 tagttaccga taatgtagcc tacagtacca attaacaaaa ttgcaccgac taaatcacgc
    11281 caacctttac tgatggctaa ggctgctgca gtagtcggcc ctccaatatt ggcattacaa
    11341 gccaatacaa tttcttcaat actgaatttg aacagcttgc ctaacactaa cgtcagtaac
    11401 aaattcaata ccgcaataat caaagtaaaa acaaagagta atggtgctgt tttaataatt
    11461 aacggtatag aggcaggaac accaatcaca acaaaaaaca gataaataca atatgtccct
    11521 aattcttgac tgccatctag tttttgaatg cctttacgga aaaataaaac aactgccaat
    11581 gtaaatgtcg ttaacagcaa atatttgtca ctaattaaac ctgtcagcat ttttaatgca
    11641 acaccatcaa cactttgaaa atgattttgc aaaaaactgg ataatgagaa tgaaactgcg
    11701 accaacaaaa tcgaggcagc caaagataag gctatactct gtagcgaaat ggatttaggt
    11761 ttccaatact cggcagcctt attcacactc ggatcatgat attcctgctc caatttatcc
    11821 tgatatggac ttccccaaac ttttctgacc caatatgagg aagagatacc aatcaaaata
    11881 aaaaagtaac aagccatcat caaattatca gcaacaattg ttgctgcaat aacatcttta
    11941 gcagggttaa gcttagctga catagctgca aaattaacac cacctcccgt ataagatgct
    12001 gaaatcatgc cagctacctt atctagttca ggaatatagt ttttcaatag tgcaaatgag
    12061 acaagcgttc caatgaccgt ccctagtgaa ctaatcagga aaataaacag catacgtcca
    12121 ctttccttaa atagagcatg aagatctaat tgaaacaata acaacggaat agcaagaggt
    12181 acaatataag accatacagc atcaaatact ggagcatccg tcggaataac acccaaatta
    12241 gaagcaatca acgcaatcaa taaagctaat actgcaccag ggactttccc agccaagcga
    12301 ctgttttgct caataaaaat cgcagcagcg gcagctaaca taacaaaagc ccacagcagc
    12361 aaatgattat ctgctgaaat taaagtttgc atttttcctc ccaattttta ttttaattta
    12421 agtatcctgc tcttctaccg ccccaaaaac tgcgttgctt tgttgggtaa aataagccca
    12481 attttggttg ccataatcga aatgccacca ttcggttggc aaattactaa atcctgcctc
    12541 tttcatcgca tgaatcaaaa tccggcgtaa attacgagca gaggagaaat actcaccttc
    12601 tttctctaaa gcatcgctat aagataaatg tccggttgca tcaaactcac ttcccatatc
    12661 caaacttttg ccacttttta tatcaaacaa tccgacatca acagatccgc ctgtcaaatg
    12721 gggcagaggc attcctgccc gatctggatc agcagcaaaa cagtccagtg cttctctaac
    12781 ttgaacttca tcatattccg gatggcgttt ttcaatttct tgcttcatat tttctcgtaa
    12841 tgctctttgc actatgacag gacgccaacc atcaaaaatt tccaatccgt acatttctgg
    12901 cagcaaagag acaacctgag tcagcctatc agcaacactt ctacgcaata aaacagtatt
    12961 tattgcacct tcaataccct gggaaaaata aacaggattg acacgaatgc gtccggtatt
    13021 accaacactg accaacggtt ccccacattc agaaataggt atttcttgta ttttctgcca
    13081 aactaactct ttggtatttt gtacgggaat accagcataa gaccccttgt ccttatccat
    13141 caaatgttcc gcattaataa ttagtatgcc aataaattca ttaaatttct tttttcagac
    13201 ggcctgttga ttcggacctt caggccgtct gaaaccataa attctgtttt attgcttgtt
    13261 atctgaaatc tattcgcggt atgaaaaaaa ttgctgctcc gctaccatca ttcccgtata
    13321 gacggaaatc caaacttaaa tctttcagca ctctttacag cagaacattt caacttttga
    13381 atttctgcct gtatgggaat gacgacagca gagcagcatg ttttccaaac ggccggctct
    13441 ctctcgcata ctgcactagg ccatcgaaaa acgaaaacat catttattca ttgttttcaa
    13501 acggcatttc gagatggggc gggctggcgg cccaccctgc tgttacgcgt attagcggtc
    13561 ttcaatctcc acaaacgcca aatcttcagg gcccgtgtag ttcgcgctcg gacggatgat
    13621 tttgccgtct ttgcgttgct cgagaacgtg tgcgctccaa ccggttgtac gagaaatcac
    13681 gaacagcggt gtgaacatag cggtcggtac gcccaatttt tgataggaaa cggcggagaa
    13741 ccagtccaga ttcgggaaca tttttttctc ttcccacatc acgctttcca aacgttcggc
    13801 aatgtcaaag aggcgcatat cgccggtttc tttgctcaaa ccgcgtgcca cttctttaat
    13861 gacaacgttg cgagggtcgg agatggtgta caccggatga ccgaaaccga tcacgatttc
    13921 tttgcggccg atgcgttcgc ggatgtcggc ttcggcttcg tcggcattgc ggtagcgttt
    13981 ttgaatatcg taagccactt cgttcgcgcc gccgtgtttc ggacctttca acgcgccgat
    14041 tgctccggta atgctggagt acatatcaga gcctgtaccg gcaatcacgc gggcggtgaa
    14101 ggtagaagcg ttgaactcgt gttcggcata cagaatcagt gaaacgtgca tggctttgat
    14161 gtgggattcg cttgggcgtt tgccgtgcaa cagttgcagg aaatgaccgc cgatggtctc
    14221 ttcgtcgctt tcaacctcaa tgcgtttgcc gttgtgcgaa tattgatacc agtacaagag
    14281 gatgctgccg aggctggcga tcagtttgtc ggcgatgtcg cgcgcttcgc tttccggatg
    14341 gctttcacgt tcaggatgaa cgcagcccag catggatacg ccggtacgca tcacatccat
    14401 cggatgggta tgtgcaggta ggctttccaa aactttaatc acacggatag gcaggccgcg
    14461 catggatttg agcttggctt tataagcggc cagctcgaat ttgttgggca aatgaccgtg
    14521 aatcagcagg tgggcgactt cttcaaactc gcatttttgt gccaaatcca agatgtcgta
    14581 accgcgatag ctcaaatcgt tgccggtacg gccgacggta cacaatgctg tattacccgc
    14641 cgcaacgccg gaaagcgcaa cggatttttt aggtttgaaa gtcggggttt gagtagtttc
    14701 agtcatcgta tttctccttt gttttttatg gattttcaat tttcagacgg ccgatgcgga
    14761 tagattggaa ggccgtctga aagcggtaaa tcatttttga aacaatttat ccagtttttg
    14821 ctcgaaggca tgatagttca gatgctcgta cagctcggca cgggtttgca tactgtccac
    14881 caccgccgcc tgagtgccat cgcgcataat cgcttcgtaa acattcagag cggctttgct
    14941 tgctgcacgg aacgatgaca gcggatacag caccagcgac acgccgtttt cagccagctc
    15001 gctttgggta taaagcggag tggaaccaaa ctcggtaatg ttcgccaaca cgggcacttt
    15061 caccgcatct gcaaattggc ggtacatgtt caaatcggtc atggcttcag ggaaaatcat
    15121 gtccgcaccg gcttcgacac aagcttgggc gcgttcgata gcggcatcca aaccttctac
    15181 cgccagcgca tcggtacgcg ccataatcac gaagttctca tcaacgcgcg catctacggc
    15241 agctttgata cggtcgacca tttcatcttt agatacaatg gctttgttcg gacggtggcc
    15301 gcagcgtttt tgcgctacct gatcttcgat gtgaaccgct gcaacaccgg cgcgttcaaa
    15361 gttgcgaatg gtacgggcaa tattgaatgc accgccccaa cccacatcga tgtccaccag
    15421 cagaggcgta tccacgttgt ccgtaatgcg tcgtgcgtcg atcagcacat cttccattgt
    15481 ggtaatgccc aaatcaggga taccgcaaga acaggctgcc acgccgccgc cggacagata
    15541 gatggctttg aaaccgcttt gggtggccaa tcgtgcaaaa taagcattga cgcaaccggc
    15601 gacggcaagc ggattcgatt ctttcacggc ttggcggaaa cgtgctccgg cagagtgttg
    15661 actcatcata tttctccttt atagactttt tttcagtatg gacaggcttc catcacattc
    15721 ggacggcaaa acacagccat ccgacgcgtc gggcatccca atccaatcat taaaaatata
    15781 tgggaaaaat tatcttattg atatttaaaa cgaatcaaag aaaaacagca gaccgttcgg
    15841 aattatgcgg caaaaccgca gacaagaaga aaacaagggg attattcaga aaaggggaaa
    15901 acatctgaat tggtttcata gtaatgttcc tttgtagtat tgtgtagttt tatttttcgc
    15961 taatctaaaa cacaagactg aaaatgtcaa acatttttaa ggagactcat tacataattt
    16021 taatatttta ttttaattcc atgattaaac tagattaatt ttgaattatt tgtttcaata
    16081 aaattgtcag gaaatcaaga aaaatttttg ataatcaaaa tcctgaaaac ttataaaata
    16141 ttttatacat aaaaacaatg tattattttt aagcaatccg atgtatttta ttatgtattt
    16201 gatttttatt taatttaata aaaatttaat ataccggcaa acaaaatgcc gtctgaaaac
    16261 ctttcagacg gcatttttaa tatcgaatca atcagcccaa ataccagaat ttcagtgccc
    16321 acaatacagc aacaatccat accataggcg gaacgtcttt ggtgcggcgg cataaaagtt
    16381 taaccacggc atagctgatg aagccgaagg cgatgccgtc tgcaatcgaa taggtaaacg
    16441 gcatgaagac aatggtcagg aatgcgggtg cggcttccgt catatcgtcc caatcaatat
    16501 ccctcgcact gcggagcatc tgcgtgccga cataaagcag ggcgggcgcg gtggcaaaag
    16561 cgggaacact tttcgccaaa ggtgaaaaca tcaggcaggc gagcatcaat acgccgacgg
    16621 taaccgccgt caggccggtc cgtccgcctg ccgatacgcc cgccgcgctt tccacataag
    16681 gcgtggtgga agaagtaccc aaagccgcac ctgccacaat ggcggtagag tctgcaagca
    16741 gtgcgcgttt caggcggggc agcttaccgt ccaccagcag cccggcacgg tgggatatgc
    16801 cgaccagcgt tccggtactg tcaaatagat cgaccaagaa gaagacgaaa atcacactga
    16861 ccatgctgac ggtaaacagg ccttcaaaat ccatctgcat aaaagtcggc gcaatgctcg
    16921 gtacttcgcc gatgatgccg tgaaattcat tcaaacccat caggctggca atgacggtaa
    16981 tggtcaagat ggtgatgatg attgcgcctt gaacgcggaa atgtcccaat acgaccacca
    17041 tagcaaaacc gaacagtgcc aacaacgcgg acggctgatg aatatcgccc aaaccgacca
    17101 aggttgccgg attggcaacg ataatgcctg cgcctttcag ggaaatcagt gccaaaaaca
    17161 aaccgatacc ggcagcaatc gacattttca aacccatagg cagtgcgttg accagcattt
    17221 ccctgacttt aaaaaagctg aacaggatga aaatcagacc ggagatgaac accgcaccca
    17281 acgcaacctg ccaaggcacg cccataccct taacgacggc aaaggtgaaa taggcattca
    17341 gccccatccc cggtgcgagt gcaatcggat agttgccgac aaaacccata acaaaacagc
    17401 cgatggcaga cgcgatacag gtagcgacga ataccgcccc catatccatg ccggtctcgc
    17461 ccaaaatcag agggttgacg ataacgatgt agcacatcgt caaaaaagtt gtcaaacccg
    17521 ccatcaactc ggtacgcacc gtcgtaccgt ttgccttcag cttaaaaatc ccgtccaaca
    17581 gtgtttgttt tgaagtgtcc atatctgaaa gtctgtatca agtggaaaaa tctgaatccg
    17641 ccgaccgcaa accgtacgaa attgcaacat cttgaaaaac atacctccca aaccggacaa
    17701 accgattcgg gaggcagcag gtttacgggc aaacagcaca ttatttgtct gaattgtgtt
    17761 gacgacgcaa aatcgcaggg atttcaaagt cgtcaagtac ggactgattg tcgaaatccg
    17821 cagcggtaag gttcatcgtg cggataccgc gattggtgcg gatcatacct tcgacattat
    17881 ggctttgctc ctgtttggac ggggcaaccg cttctacctc ccttgccgga acaaaatcga
    17941 ccgcgccttt ttctttcaga ccggtagcga taatggtaat ccggatggca tcttcgctca
    18001 tggtctcgtc ttcagccgca ccgaatttgc attccaaatc gggatgcgcg ctttggttga
    18061 cgattttcat gacttcggac aactcggaca ttttcaagca acccggagca gtcgtaatat
    18121 tgaccagcac accgcgcgct ccgtccaagg ttacatcgtc cagcagcgga ctggaaatgg
    18181 cctggtcggt cgccatacgc gcacggtcga taccttgggc ataacccgaa cccatcatag
    18241 cgataccgcg gttgctcatc acggttttca cgtcggcaaa gtcgaggttg atgatttcgc
    18301 tcgggcaagt taccacttcg gaaatgcctg cgaccgcatc gcgcaataca ttgtcggcgg
    18361 cacggaaggc ttcgcgcatc gttacgtctt cacccaatgc agtcatcagt ttgtcgttcg
    18421 ggatgatgat cagcgaatcg acgtgttctt tcaactgttc caaccctgcc tgtgcgacat
    18481 ggacgcgctt accttcatat gcgaacggtc gggtaaccac ggcaacggtc agaatgccca
    18541 aagacttggc aatctcagca acaaccggcg cggaaccggt accggtaccg ccgcccatac
    18601 cggtcgtgat aaacagcata ttcgcaccgc gaatggcttc ttcaatggct tcccggtctt
    18661 cctgggctgc cgcacggccg atatcgggat tcgcgcccgc gcccaaaccg cgtgtcagat
    18721 tcgtacccaa ctggattctc ttcgccgcat ggttttttgc cagagactgc gcatccgtat
    18781 tggcactgat aaactccaca ccgcgcacat tgttggcaac catgttattg attgcattgc
    18841 aaccgccgcc gcccaagccg attactttaa tcaccgcagg gctgactgcc gattctgcca
    18901 cgtcgtaaac aaattccatt caaaaactcc tgctcgcccc attcagagga cggtttaaat
    18961 aaattattat tcattatata agaagtatct tgctgctggc aaaatacttc tcacctgtca
    19021 aacggcaatc cacctgttca gaagctgttt tcaatccacc gtttcaatct tgccaacaaa
    19081 ccgccgccca cttccctctc ttgcactgca ccgttttccg gctgcggcaa gtttccttcc
    19141 agcttgcatg ctgcatgaag cagcccgatg gcggtagaaa agcgcggtgt gcggacgcgg
    19201 tcggacaaac cgcccatttc ttggggcgca ccggtgcgta caggcaaatc gaagattttt
    19261 tcggcaaatt ccacaatccc ggtcatcatg gacacaccgc cggtcagaac gatacccgca
    19321 ttcagcactt ctttggggaa acccgatttt tgcagctcgc ccagcactac gccaaaaatc
    19381 tcctgaatac gtgcgctgat gattgctgcc aaaaccttac tggaaacctg acgcgatgtc
    19441 cggtcaccca cgcccggaac ttcaatcatc tcacccaagc cttccgtatc gcatgatgcc
    19501 acgccataat ggattttaat gtactcggcg gcatcgagag gtgttctcaa cgatttggac
    19561 aaatctttgg taatcagatt accaccggcc ggaatgacgg acgtatggcg gatggcgccg
    19621 ttcatataaa cggcaatatc ggtcgttccg ccaccaatgt cgatgacgca tacgccgagg
    19681 tctttttcat cttcagtcag caccgcctgc ccgcttgcca acggctgaag catgatttga
    19741 tcgcttttca agccgcaccg ctcgatacat ttttggacat tctgcactgc cgtacttgca
    19801 ccggtaatga tgtgcacccg cgtatccaga cgcacaccgc tcataccgat gggctccctc
    19861 acgccaagtt gggtgtcgat aatgtagtct tgaaccacgg catcgagaat tttttgatcg
    19921 ggcgggatat tgattgcctt tgccgtttca atggcgcggt cgatgtctgc ctgcgtgact
    19981 tccccgtctt taattttaac cacaccttgc gaattgagac tgcggatgtg gttgcctgcg
    20041 atacctgtgg taacgtgggt aattttggta tccgccatca gctcggcatc attgaccgcc
    20101 tgcctgatgg cttggacggt ggcatcgata ttggttacca tgcccgcgcg caagccccgt
    20161 gaaggagcct gccccaaacc gacgatgttg attttgtcgt catcttgaac ttccccgatc
    20221 agtgcgagga ctttagacgt accgatatcc agtacgctga tgtatctttg ctgctgttcc
    20281 attgttcgtc tgctcttaaa actgattgaa atttgcgtcg caccgtttca gacggcacgg
    20341 ccgtaatctg tccgatacct gttcccacta ttcttcggat tctttttcgg gtaaaccgtc
    20401 ggaagcatag cggactgaaa atccgtcctt atacctcata tccacatagg ataaccgatt
    20461 tttattttta cgcaacagat gctgccacgc ttcggtaaaa agccggaggc gtttcatctc
    20521 gttttcccgt ccgagcctga cggtgatgcc gttgtccaaa acgacaatcc acgccgaacg
    20581 tgccgtatag gtcatctctt tgatgcccaa accctgtttt gccaaaacag tcgaaaattc
    20641 gtcataacgg cggagcattt cggcagacgt tccttccgcg cctctgaata ccggcattcc
    20701 gggtctgtcc aagcgggctt caaaaacatt gccttcgccg tccaccaagg catggtcgcc
    20761 ccaacgcgcg accggcttgc gctcggtcag gacgacctca accgtgtcgg gaaaacggcg
    20821 gcgcaccatg accgacgcaa tccacggata ccggcggtag gcctcctgtg cgccattgat
    20881 gtccgtcctc aaaatattcc catggatgta ttctttcgcc aaactgccca atgccttctt
    20941 atcggaataa accaggttgc ccttcagcga cacctgcttg acgggcagat gattcgaatt
    21001 gtaaaaccaa accagcccgg acgcagcaag cagcatcgcc atcatgacaa gcagccagcg
    21061 cgtcagccgt tccatcgctt cggcattatc ccacatgtgc ggtcttcaaa atttcaatac
    21121 ataaatcggc aaaacccacg cccgtaacgg cagcggattt cggtactaaa ctatggctcg
    21181 tcataccggg cagggtgttg atttccaaca gatagagttt gccgtcggta tctttgagga
    21241 aatcgacgcg cacgcagcct tccgcaccga ttgcctgcgc accgcgaacc gccagttcgc
    21301 gcatcaggct ttcttcggct tcggtcaaat cttccgaagg acattgataa atggtgtcgt
    21361 cgcggttgta cttggcttcg tagtcgtaaa actcggttgc gggaatgatg tgtatgccgg
    21421 gcagcccttt gccgttcagg acggggcagg aatattcgcc gccgccgata aaacgttcgg
    21481 caatgatttc gccctgaagg tgtttcaatt cttcgtaaac gcttttcaga cggccttttc
    21541 ctttgacttt taccacgcct acgctgctgc cttcggccgc cggtttcaca aacatcggca
    21601 ggcccaattt ttcttcgacg gcatcgaaat cagtgtcgtc gtgcaggacg gcgaactcgg
    21661 gaacgggcaa tcccaatgcc tgccaaatca gtttgcagcg gtatttgtcc atgccgatgg
    21721 cggatgcggc gacaccgctg ccggtatagg gaatgcccaa cagttccaat gcaccctgaa
    21781 ccgccccgtc ttcgccgtaa gtaccgtgaa ggatgttgaa tgccgtctga aaaccttgtg
    21841 ccttcaattc agacaatggg gtttctttag gatcgaaggc gtatgcgtct atgcctttgc
    21901 tttttaaagc attcaaaatg gcggtgccgc tgtccagcga gatttctcgt tcgctggaaa
    21961 aaccgcccat caatacggcc actttgccaa aattctgcat tgttttttgt tctttcctga
    22021 ttgctttatg cttgttgcca gaggtcgtct gaaacctaat ttgcggtttc agacgacctt
    22081 tatatgatgt tccgtctgtc aggcgggtgt gcctcaaatc tgtttcgaca attccagtag
    22141 cgcggcgggg acgcggttga tgcttcccgc gcccatattc aacacgatgt cgccgtcctg
    22201 caaaacgttc aacagcattt cgggcagatc ggcaacgttt tcgcagtaaa tcggctcgag
    22261 tttgcccaac acgcggatgg cgcgggcaag agcgcgggaa tcggcggcgg caatcggctc
    22321 ttcaccggcg gcataaactt cggtcagcac cagcgcgtca acggtattga ggactttggt
    22381 aaagtcttca aacaaatcgc gcgtgcgggt atagcggtgc ggctggaagg cgagtaccaa
    22441 acgtttttcc ggatacgcgc cgcgtgcggc ggaaagggtc gccgccattt cgacggggtg
    22501 gtgtccgtag tcgtccacca agagcgcggt cccgccgttt ggcaacttga tgtcgccgta
    22561 tttttggaag cggcggccga cgccttcaaa gccgagcaag cctttttgga tcgcttcaac
    22621 cgatgcgccg acttccagcg ccacgccgat ggctgccaat gcgttcagca cgttgtgtct
    22681 gccgggcata ttcagcacga cttcaaacga cccctgctca tgtcctttca tttgaacatg
    22741 gacggtgaat ttcatttgcg cgccgacgtt ttcgatgtcg gtggcgtaga tgtcggcggt
    22801 atcgtccaaa ccgtaagtag cataaggttt gctcactttg ggcaaaatcg cgcggacgtg
    22861 ttcgctgtca atacacaaaa aggctttgcc gtagaagggc atacggtgga tgaaatcgat
    22921 aaacgcctga tgcagttttt cgacgctgtg cccgtaggta tccatatggt cttcgtcgat
    22981 attggtaacg acggacataa tcggtgtcag gtgcagaaag gatgcatccg actcgtcggc
    23041 ttcggcaacg atgtattcgc ctttgcccaa gcgggcgttg gtgcctgcgg cgttgagttt
    23101 gccgccgata acgaaagtcg ggtcaagtcc tgccgcgccg aggatggagg cggtcaggct
    23161 ggtggtcgtg gttttgccgt gcgtgccggc aatggcgatg ccgtcacgga agcgcatcaa
    23221 ctccgccaac atcagggcgc gcggaataac gggaatttgc tgctccaacg cagcgacaac
    23281 ttcgggattt tcttttttga cggcggtaga ggtaacgacg acatccgcac cgttaacgtg
    23341 ttctgcggta tggccgggat aaacttgaat gcccaggctg cccaaatgct cggtagcggc
    23401 atttcgcgcc tgatccgaac cggaaacttt aaaacccaaa ttgtgcaaga cttcggcgat
    23461 gccgctcatg ccgacgccgc cgataccgac aaaatggatg ttggtaactc gatttttcat
    23521 cataatgttg cgttccggtg gattttcgat gcgtaaaggc gttattttaa agggctatcc
    23581 gcttctacgc catagttttc ttgtaaatta cagatgtttc ggcatacagg atttcatatc
    23641 gatggcaata tagttaaaaa taaggctgca cccagcggca aaccctgctt tcctgcccat
    23701 tttcaaactc gatttcatat accgaaacac cagaaaaaac aaaatataag gccgtctgaa
    23761 accggtttcg tggttttcag acggcatctg tttcgtttat cgcatcatgt catcatgccg
    23821 tacactcgat agcggcttcc gccacgtcgt ccgcactgtg cggcagtgcc aacgtacggg
    23881 cgttttctgc ccatttgaga catttttcgc ggttcaggct gccgaggatt tcggcgagtt
    23941 tttccgccgt caactgggtt tgcggcaaca gcaatcccgc ctccgcctgc accataaaac
    24001 gtgcgttggc ggtttgatgg tcatcaacgg cgtgaggata cggcactaac aacgcaccca
    24061 atcccgccgc cgtcaactcg gcaatcgtca gcgcgccggc acggcaaatc accaaatcgg
    24121 catcgcggta ggcggacacc atatcggtaa taaattccac gcattcggct ttcacgccca
    24181 gcgcgtcgta atccgcctgc aagctgccca gcttgccccg tcccgattgg tggtacatct
    24241 gcggacgcgc attgtcgggc agcaaagcca atgcctgcgg tacggttttg ttcaaaacgt
    24301 ccgcgcccaa actgccgccg accaccaaaa ttttcagacg gccttcacgc ccttggaagc
    24361 gttcggcagg cgcgggcagc ttggcaatat cggcgcggac gggattgccg accaaaccgc
    24421 cttcgtggct gaacgctttc ggaaaagcgt acaacacccg cttcgcccag cgcgacaggt
    24481 ggcggttgga caaacctgcc acggcgtttt gctcgtgaat cacaatcggc acgcctaata
    24541 gcttcgccgc caaaccgccg gggaaggtaa cgaagccgcc gaagccgatg acgcactcga
    24601 cacggtgttt gcggataatc cgctgcgctt cgcggacggt ttgatacaaa gtaaccggca
    24661 gcatcagttt gcgtttgatg ccgttgccgc gcacgccttt aatcgccagc gtttccaagc
    24721 gtatgccgta ttgcggcacg atacgctctt ccatcgaatc cttgctgccc agccaaatca
    24781 catgatggcc gcgcgcgcgc aatgaatccg ccaccgccag cgcggggaaa atatgtccgc
    24841 ccgttccgcc cgccatcagc ataaaggttt taccgcccat gatttactcc acccgataac
    24901 cgcgcatttt ccggcggttt tcataatcta tacgcaacag cagcatcatg ctgatcagca
    24961 tgaaaaagac tgacgaaccg ccataggaca tcaacggcag cgtcagacct ttggtcggca
    25021 aagcaccgat gttcacaccg atattgaaga aactttggat accgatccaa atgccgatac
    25081 ccgaagcgat ataggcgttg aaagtcaaac ccaaatcgcg cgactgcttg ccgatggaaa
    25141 acgcccgcac caccagccag ccgtaacaga atatcagcac gcacataccg aagaaaccga
    25201 attcttcggc gatgatggca aaaataaaat cggtatgcgc ttccggcaga aagccgcgtt
    25261 tgctcaaact cgcacccaaa cccataccga accactctcc gcgcccgatt gccatcagag
    25321 agtgggtaag ctggtagccg gcaccctgcg ggtctttcca cgggtccaaa aatgccacta
    25381 cccgctgcac acggtaggga gcggcggtaa tcatcagcac catcccgccc aaaacgctgc
    25441 ctaccaggac gaaaaaatat ttccacggca atcctgccaa aaacagcatt ccaacggcaa
    25501 tgacggtaat gacgacaaac gaaccgaaat ccggctgtac cattatcagc accaaaccga
    25561 acgccaccag cataatcggc aggatgatcg cccggaaacg gccgtacatt tctaatgttt
    25621 cacgacgtgc ctgcggattg gtggcggaca tgatcagatt ggccgtcccc cgccaaatcg
    25681 actgccaacc caaactttcc atgctgcgca acacttcttc acggcgcgtg aacaggcttg
    25741 ccaaataaag gatgaccgcc agcttgaaca gctcggtcgg ctggaaattc aacggaccca
    25801 aaggtatcca acgggtcgcg ccattgattt cgcgcccggc aatcaatacg actaccagca
    25861 acaggccgga tagggcaaaa atccacggca caagccgccg ccatgtcctc atcctgcaaa
    25921 gaaaccataa caaaccgctc gctatcaagc cggcaacgac gaaccccgcc tgtctggtca
    25981 aatagaaaaa ctgatcgccg ccttcttttg atgccaaata cacagaagcc gaataaatca
    26041 tcagcaggct gaacgccgtc atcagcacca ccatccacaa aagcggcgcg tcgaatttcc
    26101 tgccgtcgcg cacaatcggc ctgtcgagca gcagagtgtg gacaccgtcg cccactttta
    26161 ccaatacttc cgaaatcttc aaaaaaacca cctgccagtc tgtttgcacc tgccgcaaag
    26221 ggcaaaaatt tcagacggca gacaatgccg tccgaacata cgatacatcc caaatcggta
    26281 ttctaaatct ttacttgccg cccaacaatg acggcgtttg catttcagac ggcatcacaa
    26341 agccttaaac gcttcgataa acacttccga acggtgcgcg tagcctttaa acatatcaaa
    26401 actcgcacaa gccgggctga gcagcacgat atcgcacgct tcggcttggg catatgccgt
    26461 ctgaacggct tcttccaaag tggcgcagtc ggtcatattc agaccgcagc cgtccaaatc
    26521 gcggcggatt tgcggcgcat caacgccgat caggaacacg ccttttgcct tgccggcaag
    26581 cgcgtcgcgc agaggcgtga agtcctgccc tttgcccatg ccgcctaaaa tcacgaagag
    26641 cggattttgc aaaccggcaa tcgcggcggc ggtcgcaccg acgttcgtgc ctttgctgtc
    26701 gtcgatgaac accacgccat ttttctcgcc gattttttcc acgcggtgcg gcaggccttg
    26761 aaaggttttg acgtgttcaa gcaatgcttc gcgcggcaaa ccgacggctt cgcacaaagc
    26821 cacggcagcc atgacgttgg cggcgttgtg caagccttgc aacggaatgt ctttcgtggc
    26881 aatcaaatct tcattgcctt gtttcaggcg gcctgtctcg cgttccaacc aaaaatcggc
    26941 ttcgtgttcc aacgaaaacc attttacctc gcgccctgcc cgtttcatgg cacggcagaa
    27001 cgcatcgtcc gcattcaaaa cctgcacacc gtcgccacgg aaaatcttgg ctttggtatg
    27061 cgcgtagtcg agcaagtcgt cgtagcggtc gagatggtct tcggaaatgt tcagtaccgt
    27121 cgccgcagtc gggcgcaggc tttcggtgtt ttccagttgg aagctggaaa gctccaacac
    27181 ccacacgtcc gcctttttgc cttcgcgctg ccattccgcc tccaaaaccg gcgtgccgat
    27241 attgcccgcg ataacggtat ccagcccgca cttgatgcag agatagccga ccaggctcgt
    27301 taccgtggtt ttgccgttgc tgccggtaat cgcaattacc ttgtcgcctc ggcggttcac
    27361 aatgtccgcc agcaattcga tgtcgcccaa cacgcgtccg ccgttttgct tgaacgcctc
    27421 gatatccggc tgccgctcgc tgatgccggg gctgagtgcc agaatatcga aaccgttgtc
    27481 cagcgcatct ttcagacggc ccgtataaaa caccagcccg tcaaacatct taccgatttg
    27541 cgacacccgc tccggtttca gctccgcatc ataagcggca acctccgcgc cgtttttgcg
    27601 taggtaggca atcatggaaa tacccgtgcc gccgagtccg gcgacgagga tttttttgtt
    27661 ttggaaagtc attttggttt tgtcctaaaa caaatcatat tgggcaggag ggaatgtccg
    27721 ctcctgctca agccgctttc agacggcatc gcgagccgat taataacccg ccttcaggcg
    27781 ttggtcattg tcgcagccgt cttggtttcc gttttgacaa gccttgccaa gccattcttg
    27841 tgcaagggcg cggtcttggc gcacgccgcg tctttcggca tacatcacgc ccaaattgtt
    27901 ttgggcttga gctaacccct gttccgccgc ctgccgatac catctgaccg cttcagcatc
    27961 atcccggcgc acgccttgtc ctttgtaata cattgcgccc aaattgtatt gggctgctgc
    28021 atttccctgc tctgccgcct gcaagttttc ccgaaaatcc gatacgtcat ccgcccacac
    28081 cgcttggttc aagcccaagg caatcagggc ggcggcaagc catttgactg tctgtttcat
    28141 ggttttactt ctgttttagt ataaggcggg tttcagccac cgttaacgat agggctgggc
    28201 ggattgtcgc cgcaggttta ttgcgcgttc aaatgccgtc tgaaagatgt tcagacggca
    28261 taggttcagc ggattttgag ggtactcaaa ccgatcaaca ccaagacgat ggtaataatc
    28321 caaaagcgga cgacgacttg ggtttctttc cagccttttt gttcgtaatg gtggtggatg
    28381 ggcgccatca ggaagatgcg ttttttggtt ttcttgtacc agccgacttg aagcataacg
    28441 gacacggctt cgacgacgaa cagaccgccc ataatcacta aaacaaactc ttggcggacg
    28501 atgacggcaa cggtaccgag tgccgcgccc aatgccagcg cgccgacatc gcccataaag
    28561 acttgcgcgg gataggcgtt gaaccacaag aaaccgaggc acgcgccgca catggcggta
    28621 cagaatatag cgacttcgtt cgcaccggcg acatagggaa gctggaggta ttgggaaaat
    28681 tggtagtgtc cgctgacgta ggcgaaaatg gcgaggccgg cagcaacgag gacgacgggg
    28741 aaggcggcaa ggccgtccaa gccgtcggtg aggttgacgg cattggatgt gccgacgatg
    28801 gtcaggtaag acaacaccaa aaagccgacc acgcccagcg gcagggcgat ttgtttgaag
    28861 aacgggacaa tcaaaatatt gttggcggaa ttggcggcaa ggtaaaacaa tgccaaaccg
    28921 gcgataatgg caacgcttga ctgccacacc attttgaatt tggcggacac gccgttgggg
    28981 tctttataga cgactttgcg ccagtcgtcg taaaaaccga gtgcgcccgt ggcgagcaat
    29041 acgcccaaga gaatccagat atacgggttt gcccagttgc cccacaacag ggtggacacg
    29101 gtaatggcgg tcagaatcag cgaaccgccc atcgtcggcg tgccgttttt gacgaggtgg
    29161 gtttgcggac cgtcggtacg cactgcctgc ccgcatttga gcgcggtcag cctgcgtatc
    29221 gtccacgggc cgaacatcag ggaaaacgcc aaggcggtca acgccgccat gacggcgcgg
    29281 aatgtggtgt attgaaaaat attcagaccg gttaaccagt tgctgaaatg tgcgagccat
    29341 aaaaacatgg ggcttccttt ttttgttttt gtcgttacat gggcttgtgc ccgtttgagg
    29401 ttggcttgcc gcccggcaag gtttcggacg acttttgcag attaacgtgc agagactttc
    29461 ccaatcacgc aaatcgggta actccccgga tttttgccgc cacccaagtc taaacataaa
    29521 tcttcataaa gatacgcctg cgtctgcatt ccggcataaa acgcgccgac cagcgcggca
    29581 atcaacaata aggctttaaa aaaacgtcga cttttcattt tcacttatcc tccaatgcct
    29641 cgaccacttc ttccatctgc ataaagcgcg aacctttcac caacacggtg gcgcgttcgg
    29701 gcaaatcgtg gcgcaacact tgaatcaacg ggtctttggc ggcgaaccac aaaccgtccg
    29761 cgccaaattt ttccgccgct tcgacgctgt tgtcgccgac aaaataagcc gcttcgatgc
    29821 cttggtcgcg ggcgtacgcg ccgacttcgg cgtgcatggc ggcggcttcg tcctcaccca
    29881 gttcgcccat atcgcccatt acgaaaatac gcggcgcagg catacgcgcc aacacgtcaa
    29941 tcgcggcttt catgctgtcc gggttcgcat tataagtatc gtcaatcagg gtcgcgccct
    30001 tgatgccggc tttgacgttc agacggcctt tgatattgct gaagcctttc aaaccttccg
    30061 ccacatcgtt caaactcaaa cctgcagcca aagacagcgc ggcggcagcg gcggcgttgt
    30121 ggacattgtg gcggccggga acaggcagca ccacggcggc gcgttcgtcg ccgcacacca
    30181 aatcaaattc gcacgacaac ggtttcagca cgatattttc cgcgtggaca tcgccgctat
    30241 cgatgccgaa agtgcgcgta ttcaaattaa gcgttgccgt tttgaagaca gccatattgg
    30301 catcttcttg aggaatcagt gccatgccgt ctgaacataa gccttgataa atctcgcttt
    30361 tcgctttggc aatatcgccc actccgtcaa aaccgcagcc gacatgggcg cgcatggcgt
    30421 tgttgatcaa agcggcatcg ggtttggcga tttgtgtcaa aaccgccagt tcgccaaaat
    30481 ggttcatgcc catttcgatg acggcatagc ggtgtttttc gtttaatttc aacaaagtca
    30541 acggcaatcc gatgtggttg ttgaagttgc ctgccgtcgc caaaacggca ttatcgccga
    30601 aacggcggcg caataccgca gccaacattt ccttcaccgt cgtcttgccg cccgaaccgg
    30661 taataccgaa cacaaacggg ttcacattct cgcgccacgc cttcgccacc atttgcaacg
    30721 cggtaagcgt gtcatcgact ttcaacgcgc catccattgc aacgcaatct tcgcgcgaaa
    30781 ccacaaccgc cgccgcacca gcagccaata cgtcttcaac aaaatcatgc gcatcaaacc
    30841 gcccgcccgc caatgcgaaa aacacatcgc ccgcgcggat gtcgcggctg tcggttacga
    30901 tgcgcgacac gggtttgctt tcagacggca tcggaagctt gagggcttgg cagatgaaat
    30961 ttaggtccag tggtttcata tttactttcg ttaatattcg ggcggcggac acatcggtag
    31021 cggctgattt tttatcgcct gttttactgt ggtaaaacac agattatttt cccattctca
    31081 ttcggcattt tttctgtacg tatcattttt tagacgtatt tttagtcgat ttgccttttc
    31141 ccgcatacca cggcgcgggg tcgtcgggca gtccgtcgat aaaggcaagg ttattgcctt
    31201 cgccctgcac atcgggaaca ttcccccaaa aatcatagcc gtcatcgggc aactcgtcgg
    31261 tttcgatacc cgtccaactg ccgaatccgc gtaaaaaatt aaacgcgctg cgcctcactt
    31321 taatcatttc gcgccgcgtg tcatcggacg aaattttaac cagcggcacg ccgtagcttt
    31381 gacgcttcca cgcaccatgt gtcaacgttc gctcgccgcc tttaccgaca tgcatcaaac
    31441 cgtggtcgga aaagtaaacc aaagaccagc tttctttatt ttcattcaat atcttaactg
    31501 tatcttctaa aaatttatcg gtttgcgcga tggtggaaac atagcaggat attttttcag
    31561 tttgatactg aaaccgccgc gcatccttat ccaaacgtgt gcaaaaatca ctgtgcgaac
    31621 ccatcaggtg catcacaatc agccgaggct tcgtgcctgc atttcctatc aaaacccgtt
    31681 tgaacgccgg caacaaaagg cggtcgctca accccgcgct tttgccataa tcaccccttt
    31741 gggtaaacca cggataatcg ctgcgtaggg cataggtgga aatttcgttg gcaaaatgcc
    31801 ccaacattcc ttgattagac agccacgccg tccgaaaacc cgcctgcttc gccaagctga
    31861 cgatgttatt gttcggttct cccggcagcc ccaaagtctg cggcagcgaa agattcgtcg
    31921 cgtgggcggt cgattggtaa ccgtttatca acagcccttt ggtctgactc aaaaaaggcg
    31981 tatcgggcaa tgggaaaccg taaacattca tataatccga acgcgcgctc tcaccgataa
    32041 ccacaacata atttttatac ttgggcgcaa catgacggat atgccatgtt gacgcttttt
    32101 ttgctgcttc caaaatgtgg gcgcgcttgg cgacatattg tgccggcgcg gaagccaaat
    32161 cgtaatacag gtcgaaaata ttcaacaaca ggccggcatc aggttctcgc aaatctttat
    32221 cgctggcgat tttatccatc accgcgcagg acaaaatcaa agtcaataat atagtcagcc
    32281 atattttgct gcggcgttta tagtttttta c
//
LOCUS       DAWHSK010000002        31817 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.002, whole genome shotgun sequence.
ACCESSION   DAWHSK010000002 DAWHSK010000000
VERSION     DAWHSK010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 31817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31817
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.002"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(129..875)
                     /gene="fabG"
                     /locus_tag="WIC03_000029"
     CDS             complement(129..875)
                     /gene="fabG"
                     /locus_tag="WIC03_000029"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEZ3882611.1"
                     /translation="MSTQDLSGKIALVTGASRGIGAAIADTLAAAGAKVIGTATSESG
                     AAAISERLAQWGGEGRVLNSAEPETVENLIADIEKTFGKLDILVNNAGITRDNLLMRM
                     KEEEWDDIMQVNLKSVFRASKAVLRGMMKQRSGRIINITSVVGVMGNAGQTNYAAAKA
                     GLIGFSKSMVREVGSRGITVNCVAPGFIDTDMTRALPEETRQTFTAQTALGRFGDAQD
                     IADAVLFLASDQAKYITGQTLHVNGGMLMP"
     gene            complement(937..2502)
                     /gene="guaA"
                     /locus_tag="WIC03_000030"
     CDS             complement(937..2502)
                     /gene="guaA"
                     /locus_tag="WIC03_000030"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEZ3882612.1"
                     /translation="MTQDKILILDFGSQVTQLIARRVREAHVYCELHSFDMPLDEIKA
                     FNPKGIILSGGPNSVYESDYQADTGIFDLGIPVLGICYGMQFMAHHLGGEVQPGNQRE
                     FGYAQVKTIDSELTRGIQDGEPNTLDVWMSHGDKVSKLPDGFAVIGNTPSCPIAMMEN
                     AEKQFYGIQFHPEVTHTKQGRALLNRFVLDICGAQPGWTMPNYIEEAVAKIREQVGSD
                     EVILGLSGGVDSSVAAALIHRAIGDQLTCVFVDHGLLRLNEGKMVMDMFARNLGVKVI
                     HVDAERQFMAKLAGVTDPEKKRKIIGAEFIEVFDAEEKKLTNAKWLAQGTIYPDVIES
                     AGAKTKKAHAIKSHHNVGGLPENMKLKLLEPLRDLFKDEVRELGVALGLPREMVYRHP
                     FPGPGLGVRILGEVKKEYADLLRQADDIFIQELRNTTDENGTSWYDLTSQAFAVFLPV
                     KSVGVMGDGRTYDYVVALRAVITSDFMTAHWAELPYSLLGRVSNRIINEVKGINRVVY
                     DVSGKPPATIEWE"
     gene            complement(2602..4467)
                     /gene="msbA"
                     /locus_tag="WIC03_000031"
     CDS             complement(2602..4467)
                     /gene="msbA"
                     /locus_tag="WIC03_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HEZ3882613.1"
                     /translation="MIEKLTFGLFKKEDARSFMRLMAYVRPYKIRIIAALIAIFGVAA
                     TESYLAAFIAPLINHGFSAPAAPPELSAAAGIISTLQNWREQFTYMVWGTENKIWTVP
                     LFLIILVVIRGICRFTSTYLMTWVSVMTISKIRKDMFAKMLTLSSRYHQETPSGTVLM
                     NMLNLTEQSVSNASDIFTVLTRDTMIVTGLTIVLLYLNWQLSLIVVLMFPLLSLLSRY
                     YRDRLKHVISDSQKSIGTMNNVIAETHQGHRVVKLFNGQAQAANRFDAVNRTIVRLSK
                     KITQATAAHSPFSELIASIALAVVIFIALWQSQNGYTTIGEFMAFIVAMLQMYAPIKS
                     LANISIPMQTMFLAADGVCAFLDTPPEQDKGTLAPQRVEGRISFRNVDVEYRSDGIKA
                     LDGFNLDIRQGERVALVGRSGSGKSTVVNLLPRFVEPSAGNICIDSIDIADIKLDCLR
                     AQFALVSQDVFLFDDTLFENVRYSRPDAGEAEVLFALQTANLQSLIDSSPLGLHQPIG
                     SNGSNLSGGQRQRVAIARAILKDAPILLLDEATSALDNESERLVQQALERLMENRTGI
                     IVAHRLTTIEGADRIIVMDDGKIIEQGTHEQLMSQNGYYTMLRNISNKDAAVRTA"
     gene            complement(4606..5532)
                     /gene="fabD"
                     /locus_tag="WIC03_000032"
     CDS             complement(4606..5532)
                     /gene="fabD"
                     /locus_tag="WIC03_000032"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEZ3882614.1"
                     /translation="MSFAFFFPGQGSQSLGMMNGFAEQAIVKATFDEASAILGQDLWA
                     MINGSDAEIIGQTVNTQPIMLAAGVAVYRAYLEAGGKTPAAVAGHSLGEYTALVAAGA
                     LNFADAVKLVRLRAELMQSAVPQGVGAMAAILGLEDEQVRQICTESAQDEVVEAVNFN
                     SPGQVVIAGNAAAVGRAMTAAKEAGAKRALPLPVSVPSHCSLMKPAADKLAEALKTVE
                     IKQPQIRVIHNADVAAYDDADKIKDALVRQLYSPVRWTETVNALVSDGIAESAECGPG
                     KVLAGLAKRINKTAACSALTDAGQVAAFIEAH"
     gene            complement(5713..6033)
                     /locus_tag="WIC03_000033"
                     /pseudo
     CDS             complement(5713..6033)
                     /locus_tag="WIC03_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223069.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
     gene            complement(6080..7042)
                     /locus_tag="WIC03_000034"
     CDS             complement(6080..7042)
                     /locus_tag="WIC03_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEZ3882615.1"
                     /translation="MQYAKISGTGSYLPANRVSNDDLAQKVDTSDEWITARTGIKFRH
                     IAAENEKTSDLAAEAARRALDAAGLDSGEIDLIIVATATPDMQFPSTATIVQQKLGIT
                     NGCPAFDVQAVCAGFMYALTTANAYIKSGMAKNALVIGAETFSRIVDWNDRTTCVLFG
                     DGAGAVVLSASDTPGIIHSKLKADGNYLKLLNVPGQIACGKVSGSPYISMDGPGVFKF
                     AVKMLSKIADDVIEEAGYTAAQIDWIVPHQANRRIIESTAKHLGLSMDKVVLTVQDHG
                     NTSAASIPLALDTGIRSGQIKRGQNLLLEGIGGGFAWGAVLLQY"
     gene            complement(7069..7272)
                     /locus_tag="WIC03_000035"
     CDS             complement(7069..7272)
                     /locus_tag="WIC03_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3882616.1"
                     /translation="MPSEACLVFRRHFLRLKQRIGNPALPIHGNLHLKTICIPNHTPI
                     PVSVICTLSVLGNHRFFAYNSRI"
     gene            complement(7292..7708)
                     /locus_tag="WIC03_000036"
     CDS             complement(7292..7708)
                     /locus_tag="WIC03_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3882617.1"
                     /translation="MKTSTVVFGGFFMADNRERIQIPVLENPGIREINHFFSVSNFEK
                     KAGVLVFRIIPEPKFGNTELTIYFEEGCYLPMIQTILEDGDIEVKNLKTENYSGNTME
                     IWGDFYPIEHISKNISTIQNIISEFMIQKQTPAPII"
     gene            complement(7735..7986)
                     /locus_tag="WIC03_000037"
     CDS             complement(7735..7986)
                     /locus_tag="WIC03_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
                     /protein_id="HEZ3882618.1"
                     /translation="MKTLEKRMKALDKRMMKFGKSLEGRLDARLIESALDYIHYSERF
                     LAFEILCTYIEDFDVRLTEQESREISFINKEFEIESTSD"
     gene            complement(8122..9177)
                     /gene="plsX"
                     /locus_tag="WIC03_000038"
     CDS             complement(8122..9177)
                     /gene="plsX"
                     /locus_tag="WIC03_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEZ3882619.1"
                     /translation="MITLAVDAMGGDQGLAVTVPGATAFLQAHPDVRLIMTGDETQLR
                     QALTAAGAPMERIDICHTTQVVGMDESPQSALKNKKDSSMRVAINQVKEGKAQAAVSA
                     GNTGALMATARFVLKTIPGIERPAIAKFLPSDTDHVTLALDLGANVDCTPEQLAQFAV
                     IGSELVHALHPQKGQPRVGLVNVGTEDIKGTDTVKQTYKLLQNSKLNFIGNIESNGIL
                     YGEADVVVADGFVGNVMLKTIEGAVKFMSGAIRREFQSNLFNKLAAVAALPALKGLKN
                     KLDPRKFNGAILLGLRGIVIKSHGGTDETGFRYALEEAYHEAKSAGLSKIEQGVAEQL
                     AALETAKAVQNENVGGL"
     gene            complement(9265..9792)
                     /locus_tag="WIC03_000039"
     CDS             complement(9265..9792)
                     /locus_tag="WIC03_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HEZ3882620.1"
                     /translation="MKQKIWYTYDDIHRVIKALAEKIRNADIKYDAMIAIGGGGFIPA
                     RMLRCFLEIPIYAVTTAYYDSDNEGQVTEEVKKVQWLDPVPEALRGKNVLVVDEVDDS
                     RVTMEFCLTELLKEDFGTIGVAVLHEKIKAKAGKIPEGIPYFSGITVEDWWINYPWDA
                     LDIDEHNRLAEAGRG"
     gene            complement(9967..10146)
                     /gene="rpmF"
                     /locus_tag="WIC03_000040"
     CDS             complement(9967..10146)
                     /gene="rpmF"
                     /locus_tag="WIC03_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEZ3882621.1"
                     /translation="MAVQQNKKSPSKRGMHRSHDTLTAPALSVDSTTGEVHRPHHISP
                     NGMYRGRKVVKAKGE"
     gene            complement(10180..10683)
                     /locus_tag="WIC03_000041"
     CDS             complement(10180..10683)
                     /locus_tag="WIC03_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEZ3882622.1"
                     /translation="MSDPNLIDPEIFAAEGQNLQGSFLLEELDERVSSHDYPADRQTK
                     ISFTLTGGRDRLQRLFLDLNVKADMPLICQRCIKPMPFMLDESSRIVLFSNEESLDES
                     MLADEELEGILIEKELDVRTLVEDQILMSLPFSPRHEDCGDNGTLEEVNRDKPNPFAV
                     LAGLKSN"
     gene            10831..11421
                     /gene="maf"
                     /locus_tag="WIC03_000042"
     CDS             10831..11421
                     /gene="maf"
                     /locus_tag="WIC03_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation protein Maf"
                     /protein_id="HEZ3882623.1"
                     /translation="MGLELPLILGTSSVFRREQMERLGIAFQAASPDFDETPMLGESA
                     PQTALRLAEGKARSLTGRFPKALIVGADQVAWCDGRQWGKPMNLANAQKMLMHLSGRE
                     IEFYSAVVLLNTVTGRMQRHIDKTVVVMRQLDELHILRYLEREPDAVYCSCALKSEDL
                     GALLIERIESTDPNALIGLPVFRLVDFLKNEGVDVL"
     gene            11480..12193
                     /locus_tag="WIC03_000043"
     CDS             11480..12193
                     /locus_tag="WIC03_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEZ3882624.1"
                     /translation="MMSPVLYLIPTPLGTPDTPCLLPHEQQAIVGLTDFVVEAEKTAR
                     AHLKHLGVTMPIRELNLQTLNEHTDLKTLLELLKPLQEGRSMGIVSEAGCPAVADPGA
                     NLVALAHKHGFEVRPLVGPSSLLLALMASGANGQSFAFKGYLPSEKNERIQSLKALEQ
                     RSRQQNETQLFIETPYRNDVLLADAVENLHSETRLCTATDLTLPTQEIISQTVVQWRK
                     RKEMPNLKKRPTIFVMYAG"
     gene            complement(12274..13911)
                     /gene="yidC"
                     /locus_tag="WIC03_000044"
     CDS             complement(12274..13911)
                     /gene="yidC"
                     /locus_tag="WIC03_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEZ3882625.1"
                     /translation="MDFKRLTAFFAIALVIMIGWEKMFPTPKPVPAPQQTAQQQAVTA
                     SAEAALAPATPITVTTDTVQAVIDEKSGDLRRLTLLKYKATGDENKPFILFGDGKEYT
                     YVAQSELLDAQGNNILKGIGFSAPKKQYSLEGDKVEVRLSAPETRGLKIDKVYTFTKG
                     SYLVNVRFDIANGSGQTANLSADYRIVRDHSEPEGQGYFTHSYVGPVVYTPEGNFQKV
                     SFSDLDDDAKSGKSEAEYIRKTPTGWLGMIEHHFMSTWILQPKGGQSVCAAGDCRIDI
                     KRRNDKLYSTSVSVPLAAIQNGAKSEASINLYAGPQTTSVIANIADNLQLAKDYGKVH
                     WFASPLFWLLNQLHNIIGNWGWAIIVLTIIVKAVLYPLTNASYRSMAKMRAAAPKLQA
                     IKEKYGDDRMAQQQAMMQLYTDEKINPLGGCLPMLLQIPVFIGLYWALFASVELRQAP
                     WLGWITDLSRADPYYILPIIMAATMFAQTYLNPPPTDPMQAKMMKIMPLVFSVMFFFF
                     PAGLVLYWVVNNLLTIAQQWHINRSIEKQRAQGEVVS"
     gene            complement(14083..14304)
                     /gene="yidD"
                     /locus_tag="WIC03_000045"
     CDS             complement(14083..14304)
                     /gene="yidD"
                     /locus_tag="WIC03_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEZ3882626.1"
                     /translation="MNFLLSKLLLGLIRFYQYCISPLIPPRCRYTPTCSQYAVEAVKK
                     YGAFKGGRLAIKRIARCHPFGGHGHDPVP"
     gene            complement(14370..14735)
                     /locus_tag="WIC03_000046"
     CDS             complement(14370..14735)
                     /locus_tag="WIC03_000046"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEZ3882627.1"
                     /translation="MDYRFGRQYRLLKTDDFSSVFAFRNRRSRDLLQVSRSNGNGLDH
                     PRIGLVVGKKTAKRANERNYMKRVIRDWFRLNKNRLPPQDFVVRVRRKFDRATAKQAR
                     AELAQLMFGNPATGCRKQA"
     gene            complement(14738..14872)
                     /gene="rpmH"
                     /locus_tag="WIC03_000047"
     CDS             complement(14738..14872)
                     /gene="rpmH"
                     /locus_tag="WIC03_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEZ3882628.1"
                     /translation="MKRTYQPSVTKRKRTHGFLVRSKTRGGRAVLAARRAKGRKRLAV
                     "
     gene            15160..16716
                     /gene="dnaA"
                     /locus_tag="WIC03_000048"
     CDS             15160..16716
                     /gene="dnaA"
                     /locus_tag="WIC03_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEZ3882629.1"
                     /translation="MTLAEFWPLCLRRLHDMLPHGQFAQWIAPLTVGEEGGVWVVYGK
                     NQFACNMLKSQFAGKIEAVREELAAGRSAFVFKPGEGVCYEMAAVEGAVEPAEPSLHA
                     GSEEMPVQEVLLDELPSEEPVKPAASKTAADILAERMRNLPHEPRQAAGSASRPESAA
                     VAKARTDAQRDAEEARYEQTNLSPDYTFDTLVEGKGNRLAAAAAQAIAESPGQSYNPF
                     FLYGSTGLGKTHLVQAVGNELLKNRPDAKVRYMHSDDYIRSFMKAVRNNTYDVFKQQY
                     KQYDLLIIDDIQFIKGKDRTMEEFFYLYNHFHNEKKQLILTCDVLPAKIEGMDDRLKS
                     RFSWGLTLELEPPELEMRIAILQKKAEAAGISIEDEAALFIANLIRSNVRELEGAFNR
                     VGASSRFMNRPVIDIDLARTALQDIIAEKHKVITADIIIDAVAKYYRIKISDVLGKKR
                     TRNIARPRQVAMSLTKELTTLSLPSIGDSFGGRDHTTVMHGIRAVAKLREEDPELAQD
                     YEKLLILIQN"
     gene            16951..18054
                     /locus_tag="WIC03_000049"
     CDS             16951..18054
                     /locus_tag="WIC03_000049"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEZ3882630.1"
                     /translation="MLILQAERDSLLKPLQAVTGIVERRHTLPILSNVLIEGKGGQTK
                     LLATDLEIQIDTAGPEGGAGDFRITTNAKKFQDILRALPAGALVSLDWDDSRLTLKAG
                     KSRFALQTLPAADFPMMNVGEDISATFSLEQERFKTMLSQVQYSMAVQDIRYYLNGLL
                     MQVEGSQLRLVATDGHRLAYAACAIDADLPHAEVILPRKTVLELFKLLNNPDDPIQIE
                     LLDKQVRFQCNGTTIVSKVIDGKFPDFNRVIPLDNDKIFVLSRAELLGALERVSILAN
                     EKFRGARLFLQSGLLSVVCSNNEQEEAREEIEIAYQGGELEVGFNIGYLMDVLRNIHS
                     DDMQLAFGDANRSTLFTVPNNPNFKYIVMPMRI"
     gene            complement(18023..18292)
                     /locus_tag="WIC03_000050"
     CDS             complement(18023..18292)
                     /locus_tag="WIC03_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3882631.1"
                     /translation="MPSETFPFQTAWFYSNLNRADRSQKHTFAIPAQALPLYAPNPKP
                     PHSRRHYPSDSRTKPRIRLHGSGHISNTGIVFRKTVKSASASQYI"
     gene            complement(18317..20374)
                     /gene="ppk1"
                     /locus_tag="WIC03_000051"
     CDS             complement(18317..20374)
                     /gene="ppk1"
                     /locus_tag="WIC03_000051"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEZ3882632.1"
                     /translation="MPEQNRILCRELSLLAFNRRVLAQAEDRNVPLLERLRFLCIVSS
                     NLDEFFEVRMAWLKRENKLHPRRRLDNGKMPSETIADVTEAARSLIRHQYDLFNNVLQ
                     PELAQEGIHFYRRRNWTGAQKKWIESYFDRELLPILTPIGLDPSHPFPRPLNKSLNFA
                     VELDGTDAFGRPSGMAIVQAPRILPRVVPLPSELCGGGHGFVFLSSILHAHVGKLFPG
                     MNVKGCHQFRLTRDSDLTVDEEDLQNLRAAIQNELHDREYGDGVRLEVADTCPAYIRD
                     FLLAQFKLTAAELYQVKGPVNLVRLNAVPDLVNRPDLKFPTHTPGRLKALGKTASIFD
                     LVRQSPILLHHPYQSFDPVVEMMREAAADPDVLAVKMTIYRTGTRSELVRALMKAALA
                     GKQVTVVVELMARFDEANNVNWAKQLEEAGAHVVYGVFGYKVHAKMALIIRREDGVLK
                     RYAHLGTGNYHQGTSRIYTDFGIITADEQITADVNTLFMEITGLGKPGRLNKLYQSPF
                     TLHKMVIDRIARETEHAKAGKPARITAKMNSLIEPTVIEALYRASAAGVQIDLIVRGM
                     CTLRPGVKGLSENIRVRSIIGRQLEHARVYCFHNNGADDTFISSADWMGRNFFRRIET
                     AAPIAAPELKKRVIREGLEMALDDNTHAWLMQPDGGYIRAAPAEGESEADLQNDLWTL
                     LGG"
     gene            complement(20543..20986)
                     /locus_tag="WIC03_000052"
     CDS             complement(20543..20986)
                     /locus_tag="WIC03_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HEZ3882633.1"
                     /translation="MPFRLFLGILCGLSMVAPVYAQGQPDAVNAYIQKKKVIVNTSKA
                     ELCFADDRQCHPVLIGVATPKGTFGLTLNSTDKPGYGGEVIGFKQEGDFLFALHRVWN
                     QIPSERRSERIASSVVSDRIMTNGCINVSDAVYEKLRHYFVLEVI"
     gene            complement(20993..21508)
                     /locus_tag="WIC03_000053"
     CDS             complement(20993..21508)
                     /locus_tag="WIC03_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEZ3882634.1"
                     /translation="MKIKQIVKPGLAVLAAGVLSACATKSNVKADGTTDNPVFPKPYS
                     VTLDNKRGTFPTYDELDQMRPGLTKDDIYKILGRPHYDEGMYGVREWDYLFHFHTPGV
                     GIDPENTSGVEDVTTCQYKVIFDKDKFARSFYWNPVFPKDAACPPPAPKAEPQVIIRE
                     IVPAKPKRIRQ"
     gene            21775..24405
                     /locus_tag="WIC03_000054"
     CDS             21775..24405
                     /locus_tag="WIC03_000054"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEZ3882635.1"
                     /translation="MQEHYQPAAIEPAAQKKWDDARIFNVSEDASKPKYYCLSMFPYP
                     SGKLHMGHVRNYTIGDVLSRFKRLNGFNVMQPMGWDAFGMPAENAAMKNNVAPAAWTY
                     DNIEYMKTQLKSLGFAIDWARETATCKPEYYRWEQWLFTKLFEKGIVYRKNGTVNWDP
                     VDQTVLANEQVIDGRGWRSGALIEKREIPMYYFKITDYAEELLNDLDKLEHWPEQVKT
                     MQRNWIGKSRGMTVRFAVSDDSKQGLEGDYAKFLQVYTTRHDTLMGATYVAVAAEHPL
                     ATAAAADKPELQAFIAECKAGSVAEADMATMEKKGVPTGRYVVNPLNGDKLEVWIANY
                     VLWGYGDGAVMAVPAHDERDFEFAAKYSLPKKQVIAVGDNAFDANRWQEWYGDKENGV
                     LVNSGDLDGLDFQTAFDAVAAKLQSQGAGEPKTQYRLRDWGISRQRYWGCPIPIVHCE
                     QCGDVPVPADQLPVVLPENVVPDGMGSPLAKMPEFYETACPSCGGAAKRETDTMDTFM
                     ESSWYFFRYMSPKFSDGMVSAEAAKYWGAVDQYIGGIEHAILHLLYARFFTKLMRDEG
                     LVNVDEPFERLLTQGMVVCETYYRENDKGGKDWINPADVELTFDDKGRPISAVLKADG
                     LPVVISGTEKMSKSKNNGVDPQELINAYGADTARLFMMFAAPPEQSLEWSDSGVEGAH
                     RFLRRLWRTVYEYLKQGEAVKAFAGSQDGLSKELKDLRHKLHATTAKVSDDYGRRQQF
                     NTAIAAVMELLNQYDKTDTGGEQGRAVAQEVLETAVRLLWPIVPHICETLWSELNGAK
                     LWEAGWPTVDEAALVKSEIEVMVQVNGKLRGKITVAADASKADLEAAALATEGAVKFM
                     EGKPAKKIIVVPGRLVNIAV"
     gene            24635..25426
                     /locus_tag="WIC03_000055"
     CDS             24635..25426
                     /locus_tag="WIC03_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEZ3882636.1"
                     /translation="MNLFFDTKLGKQQNKATHKIRVMSEAWLEKNGYCPCCGSKPMQR
                     FANNKPVADLFCSNCHEQYELKSKNQKTIGNSVPDGAYRTMLERIRSDTNPNFFFLAY
                     KKADYSIRQLVLVPKHFITPDMIIPRNKGIKNRPNHIMCSINLAPLPESGKIFLIDDS
                     RIIEPETVLKKWQSNLFLRNQNAERKGWLLAVMKCIDQLPEEFTLSQMYEFENKLSIQ
                     FPQNKHIKDKIRQQLQILRDQNTIEFIGRGLYKKIDKLPPTSKAF"
     gene            25556..25687
                     /locus_tag="WIC03_000056"
     CDS             25556..25687
                     /locus_tag="WIC03_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl-tRNA synthetase"
                     /protein_id="HEZ3882637.1"
                     /translation="MRNFDKLAEEAAEHAAEGAVKFIDGKPAKKIIAVPGRLANIVV"
     gene            25710..26644
                     /locus_tag="WIC03_000057"
                     /pseudo
     CDS             25710..26644
                     /locus_tag="WIC03_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225798.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
     gene            complement(27027..27338)
                     /locus_tag="WIC03_000058"
     CDS             complement(27027..27338)
                     /locus_tag="WIC03_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HEZ3882638.1"
                     /translation="MKYKSEALAAIHEMMEGAYNIGAIDKKTMRGFDKSCLTEIKPLS
                     GGDIKAIREKEALSQAAFAIYLNVGKNHVSAWERGVKKPSGAALKLLTIVKNKGIEAI
                     A"
     gene            complement(27335..27703)
                     /locus_tag="WIC03_000059"
     CDS             complement(27335..27703)
                     /locus_tag="WIC03_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEZ3882639.1"
                     /translation="MRIFKNQWIVKFAKKHKINDSELLEAVERADNGLIDADLGGGVI
                     KQRIARQGQGRSGGYRSLILFKQADRAFFVYAFAKNDRENISDKELDVYRKAAAYYLK
                     YTRAELAALKEDGIITEIES"
     gene            complement(28513..28598)
                     /locus_tag="WIC03_000060"
     tRNA            complement(28513..28598)
                     /locus_tag="WIC03_000060"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(28562..28564),aa:Leu,seq:gag)
     gene            complement(28612..28962)
                     /gene="secG"
                     /locus_tag="WIC03_000061"
     CDS             complement(28612..28962)
                     /gene="secG"
                     /locus_tag="WIC03_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEZ3882640.1"
                     /translation="MEAFKTLIWIVNIISALAVIVLVLLQHGKGADAGATFGSGSGSA
                     QGVFGSAGNANFLSRSTAVAATFFFATCMAMVYIHTHTTKHGLDFSNVQQTQQAPKPV
                     SNTEPSAPVPQQQK"
     gene            complement(28969..29742)
                     /locus_tag="WIC03_000062"
     CDS             complement(28969..29742)
                     /locus_tag="WIC03_000062"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEZ3882641.1"
                     /translation="MYRQIGMWDQKWVIGNWKMNGRLQNNNALMHRFRILPTAERVLI
                     GLAAPTVYLLQLHNAMQIVLNNRILTCAQDVSRFPDNGAYTGEVSAEMLADTGTDIVL
                     IGHSERSLYFGEKNEIQRRKMENVLNVGLIPLLCVGESLEEREAGKEHEVIAHQLSIL
                     QGLDTKNIAVAYEPVWAIGTGKVATVEQIADMHAFIYKEILSLCGSDVKIRVLYGGSV
                     KADNAADIFAVPYVDGALVGGASLSYDSFTAIISAAQNA"
     gene            29926..30429
                     /locus_tag="WIC03_000063"
     CDS             29926..30429
                     /locus_tag="WIC03_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEZ3882642.1"
                     /translation="MHALHFSASDKAALYREVLPQIESVVADEADWVANLANTAAVLK
                     EAFGWFWVGFYLVDTRSDELVLAPFQGPLACTRIPFGRGVCGQAWAKGETVVVGDVNA
                     YPDHIACSSLSRSEIVVPLFSDGRCIGVLDADSEYLAQFDETDALYLGELAKILEKRF
                     EASRQAV"
     gene            30531..31187
                     /locus_tag="WIC03_000064"
     CDS             30531..31187
                     /locus_tag="WIC03_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEZ3882643.1"
                     /translation="MDFEKARFNMVEQQIRPWDVLDFDVLDALAEIPRELFVDEDLQG
                     LAYADMALPLANGHKMLEPKVVARLAQGLKLTKNDTVLEIGTGSGYAAALLAKLAGRV
                     VSDDIDAEQQKRAKAVLDGLSLENIDYVQNNGLTELSAGAPFDAVYVGGAVNLVPEVL
                     KEQLKDGGRMAVIVGRKPVQRALLITRKGDVFEEKVLFDTLVAHLDDKDADPFGGFDF
                     "
     gene            31267..31590
                     /locus_tag="WIC03_000065"
     CDS             31267..31590
                     /locus_tag="WIC03_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEZ3882644.1"
                     /translation="MDIVQLGPAELKAWMDEGRMFCLLDVRTDEETAVCSLPNALHIP
                     MNLIPLRQNELPDDVPLVVYCHHGIRSLHTAMYLADAGFENLYNLQGGIDAWAAEIDG
                     NMMRY"
BASE COUNT         6855 a         7705 c         9351 g         7906 t
ORIGIN      
        1 ttttgcaaag gtctcagctt gtgcctgatc cgcaggggca gggcggggtg tggcgtgtgt
       61 tcattttgcc tgagaatgca aatgccgtct gaaagggctt cagacggcat tgtcggtacg
      121 gctgcccgtc agggcatcag cataccgccg ttgacgtgca gcgtttggcc ggtgatgtat
      181 tttgcttggt cggaagcgag gaacagaacc gcatcggcga tgtcttgcgc gtcgccgaat
      241 ctgcccaagg cggtttgggc ggtaaaggtt tggcgggttt cttccggcag ggcgcgtgtc
      301 atgtcggtat cgataaagcc aggggcgacg cagttgacgg taatgccccg gctgccgact
      361 tcgcgcacca tggatttgga gaaaccgatt aagcctgctt ttgccgcggc atagttggtt
      421 tgaccggcat tgcccatcac gccgacgacg gatgtgatgt tgatgatgcg gccggaacgt
      481 tgtttcatca taccgcgcaa aacggcttta gaagcgcgga acacggattt gaggttgacc
      541 tgcatgatgt cgtcccactc ttcttctttc atgcgcatca ggaggttgtc gcgggtgatg
      601 ccggcgttgt tgaccagaat atcgagtttg ccgaacgttt tttcgatgtc ggcaatcagg
      661 ttttcgacgg tttcgggttc ggcggaattt aatacgcggc cttcgccgcc ccattgcgcc
      721 aaccgctcgc taatcgccgc cgcaccgctc tcactggtcg ccgtaccgat gactttggca
      781 ccggctgccg ccagtgtgtc ggcaattgct gcaccgatac cgcgcgatgc gccggttacc
      841 aaagcgattt tgccgcttaa atcttgtgtg ctcatatttt ttcctttttt ccgataatcg
      901 aaagactgcg ccggacggtg cagccctgtt tgctggttat tcccactcga tggtggcagg
      961 cggtttgccg ctgacatcgt acaccacgcg gttgatgcct ttgacttcgt tgatgatgcg
     1021 gttggacacg cgtccgagca gtgagtaagg cagttctgcc cagtgtgcgg tcatgaagtc
     1081 gctggtgatg actgcgcgca gtgcgacgac gtagtcgtaa gtgcgtccgt cgcccattac
     1141 gccgacggat ttcacaggca ggaatacggc aaatgcctgg ctggtcaggt cgtaccaaga
     1201 tgtgccgttt tcgtcggtgg tgttgcgtaa ttcttggatg aaaatatcgt ctgcttgacg
     1261 caataagtcg gcgtattctt ttttcacttc gcccaagatg cgcacgccca aaccggggcc
     1321 ggggaacgga tggcggtaca ccatttcgcg cggcaagccc aaagcaacgc cgagttcgcg
     1381 cacttcgtct ttgaacaagt cgcgcaacgg ctcaagcagc ttgagcttca tgttttcggg
     1441 caggccgccg acgttgtggt gcgatttgat ggcgtgggct tttttggttt tcgcgcctgc
     1501 agattcgatt acgtcagggt aaatcgtacc ttgcgccagc catttggcgt tggtgagttt
     1561 tttctcttcg gcatcaaata cttcgataaa ttccgcgccg atgattttgc gttttttctc
     1621 aggatcggtc acgccggcga gtttcgccat aaactgcctt tcggcatcga cgtgtatcac
     1681 tttcacaccc aagttgcggg cgaacatatc catcaccatt ttgccttcgt tcaggcgcaa
     1741 caaaccgtga tcgacgaaca cgcaggtcag ttggtcgccg atggcgcggt gaatcagcgc
     1801 ggcggctacg gaagagtcta cgccgcctga caaacctaaa atcacttcgt cgctgccgac
     1861 ctgttcgcgg attttggcaa cggcttcttc gatgtagttc ggcatcgtcc agcccggttg
     1921 tgcgccgcaa atatccaaga caaagcggtt caacagggcg cggccttgtt tggtgtgggt
     1981 aacttcgggg tggaactgga tgccgtagaa ttgtttttcg gcgttttcca tcatggcaat
     2041 cgggcaggac ggggtgttgc cgatgacggc gaaaccgtcg ggcagtttgg acactttgtc
     2101 gccgtggctc atccatacgt cgagtgtgtt tggctcacca tcttgaatgc cgcgtgtcag
     2161 ctcgctgtct atggttttaa cttgcgcata accgaattcg cgctggttgc cgggctgcac
     2221 ttcgccgccc aagtggtgcg ccataaactg catgccgtaa cagatgccca aaaccggaat
     2281 gcccaaatca aaaataccgg tatcggcttg atagtcggat tcgtaaacgg aattggggcc
     2341 gccagagagg atgatgcctt tggggttgaa ggctttgatt tcgtccaaag gcatatcgaa
     2401 ggaatgcagt tcgcagtaaa cgtgggcttc gcgcacgcgg cgggcaatca gctgggtaac
     2461 ttgagaaccg aagtcgagga tgaggatttt gtcttgggtc ataatgggtt ttacagtgat
     2521 tgaagataat cggatgggtc ggcggatatg atgccgtctg aaagggtcgg ggcgattgta
     2581 ccatttcgga cggcattttg tttatgccgt ccggacggcg gcatctttgt ttgagatatt
     2641 gcgtaacatt gtgtagtagc cgttttggga catcagttgt tcgtgtgtgc cttgttcgat
     2701 gattttgccg tcgtccatca cgatgatgcg gtcggcccct tcgatggtgg tcaggcggtg
     2761 ggcgacgatg atgccggtgc ggttttccat caggcgttcg agcgcttgtt ggacgaggcg
     2821 ttcggattcg ttgtctaatg cgctggtggc ttcgtccaat aataatatcg gcgcgtcttt
     2881 caaaatggcg cgggcaatgg cgacgcgttg ccgctgtccg ccggataagt tgctgccgtt
     2941 cgatccgatg ggctggtgca gtccgagcgg ggagctgtca atcaggcttt gcaggttggc
     3001 ggtttggagg gcgaacagga cttcggcttc gcctgcgtcg ggacggctat atcggacgtt
     3061 ttcaaaaagg gtatcgtcaa acaggaatac gtcttgggag acgagggcga attgggcgcg
     3121 caggcagtcg agtttgatgt cggcgatgtc gatactgtct atgcagatgt tgccggcaga
     3181 cggttcgaca aagcggggca gcaggttgac gacggtggat ttgccgctgc cggaacgtcc
     3241 gaccagggcg acgcgttcgc cttgtctgat gtcgaggttg aagccgtcga gggctttgat
     3301 gccgtctgaa cggtattcga catcgacgtt gcggaagctg atgcgccctt cgacacgctg
     3361 cggcgcgagc gtgcctttgt cctgttcggg cggggtgtcg agaaatgcac atacaccgtc
     3421 ggcggcgagg aacatcgtct gcatagggat gctgatgttg gcaaggcttt tgatgggggc
     3481 gtacatttgc agcatcgcga cgatgaatgc cataaattcg ccgatggtgg tgtagccgtt
     3541 ttggctttgc cacagggcga tgaagatgac gacggcgagg gcgatcgagg cgatcagttc
     3601 gctgaacggg gaatgtgccg ccgttgcctg cgtgattttt ttgctgaggc ggacgatggt
     3661 gcggttgacc gcgtcgaacc ggtttgccgc ctgcgcctgc ccgttgaaca gcttgacgac
     3721 gcggtgtccc tgatgggttt cggcaatcac gttgttcatc gtgcctatgc ttttttgcga
     3781 gtcggaaatg acgtgtttca gacggtcgcg gtagtagcgc gagagcaggg agagcagggg
     3841 gaacatcagg acgacgatga ggctgagctg ccagttgagg taaagcagga cgatggtcag
     3901 gccggtaacg atcatcgtgt cgcgcgtgag gacggtgaag atgtcgctgg cgttgctgac
     3961 cgactgttcg gtcaggttga gcatattcat cagtacggtg ccggacggcg tttcctgatg
     4021 gtagcgggag gaaagggtca gcattttggc aaacatatct ttgcggattt tgctgatggt
     4081 catcacggag acccaagtca tcagataggt gctggtaaag cggcagatgc cacggatgac
     4141 gacgaggatg atgaggaaga gcgggacggt ccagattttg ttttccgtcc cccaaaccat
     4201 ataggtaaac tgttcgcgcc agttttgcag ggtggaaatg atgccggcgg cggcagacag
     4261 ctcgggcggc gcggcaggtg cggaaaagcc gtggttaatc aggggggcga tgaaggcggc
     4321 aaggtagctt tcggtggcgg caacgccgaa aatggcaatc agggcggcaa tgatgcggat
     4381 tttgtagggg cggacgtatg ccatcaggcg cataaagctg cgcgcgtctt cttttttaaa
     4441 cagtccgaaa gtcagttttt ctatcatatc ggtggggcgg aaactgcaac ggcagccgct
     4501 gtcggtggtt tggtcgggat ggattatagc gtaaatatgc ggctgtttgc cgttcggacg
     4561 tgaaaagcag cctgaagagg gcaggctgct ttttgcggaa cgaagtcagt gcgcttcgat
     4621 aaaggcggca acctgtccgg catcggtcag cgcgctgcac gcggcggttt tgttgatgcg
     4681 ttttgccaag cccgccaaca ctttgcccgg tccgcattcg gcggattcgg caatgccgtc
     4741 tgaaacaagg gcgttgacgg tttccgtcca gcgtacgggg ctgtaaagct ggcggacgag
     4801 cgcgtctttg attttgtcgg catcatcgta ggcggcaacg tcggcgttgt ggatgacgcg
     4861 gatttgcggc tgcttgattt cgacggtttt gagggcttcg gcgagtttgt cggcggcggg
     4921 tttcatgagg ctgcaatggg aaggtacgga cacgggcagc ggcagggcgc gtttggcacc
     4981 ggcttctttg gcggcggtca tggcgcgtcc gacggcggcg gcattgcctg caattacgac
     5041 ttgtccgggc gagttgaagt tgacggcttc aaccacttca tcctgtgcgg attcggtgca
     5101 aatctgcctt acctgttcat cttccaaacc caaaattgcc gccattgcgc ccacgccttg
     5161 cggtacggcg gactgcatca gttcggcgcg taggcgcacg agtttgaccg cgtcggcaaa
     5221 attcaatgcg ccggcggcaa cgagtgcggt gtattcgccg aggctgtgtc cggcaacggc
     5281 ggcaggcgtt ttgccgcccg cttccaaata ggcgcggtaa acggcaacgc cggcggcgag
     5341 cataatgggc tgggtgttga cggtttgacc gatgatttcg gcatcgctgc cgttaatcat
     5401 cgcccacaag tcctgcccca atatggcgga agcttcgtca aatgtggctt taacgatggc
     5461 ttgctcggca aagccgttca tcatgcctag gctttgtgag ccttgtccgg gaaagaagaa
     5521 tgcaaaagac atagtattcc tttgtttggt tggataatca ggtattctgc ccgagtttta
     5581 cgaaaaattc aggcgggaca aaataatata gtggattaac aaaaatcagg acaaggcgac
     5641 gaagccgcag acagtacaaa tagtacggca aggcgaggca acgccgtact ggtttaaatt
     5701 taatccacta taaatcagga tgagggtcgc caggacattg cccgaaacag tcagacggaa
     5761 cagcacaaca aaacgctttt tcgccgtttt atgcctgaat atcctgctgc ccaagtatgc
     5821 gcccgcccaa ccgccgaaca aggcaggcag cagcaggcgg tgttcgggaa tgcggcgttt
     5881 tccccgcacg gcacgccgtt tgtcgatgcc gtaaagtgca aacgcataga gggacaatat
     5941 tgcgtaacac gcgcccaata cggggaggcg cagcgaaacg gcagacagaa atgccgcaca
     6001 cgccatcagt ttgaagaaag cctgccgctt catgatatgg gaaatgccgt ctgaaagcct
     6061 gtttcagacg gcatcgggtt caatattgca acagcaccgc gccccacgcg aaaccgccgc
     6121 cgatgccttc gagcagcagg ttttgaccgc gtttgatttg tccgctgcgg atgcccgtat
     6181 ccaaagccag cggaatcgat gcggcggatg tgttgccgtg gtcttggacg gtcaggacga
     6241 ctttgtccat actcaaacct aaatgtttcg cggtcgattc gataatgcgg cggtttgcct
     6301 gatgcggcac gatccagtcg atttgagcgg cggtataacc ggcttcttcg ataacgtcat
     6361 cggcgatttt ggacagcatt ttgacggcaa acttgaacac gccgggaccg tccatcgaaa
     6421 tgtacggcga accggaaact ttgccgcagg cgatttgccc ggggacgttt aagagtttca
     6481 gataattgcc gtcggccttg agtttgctgt ggatgatgcc cggcgtgtcc gacgcgctta
     6541 aaaccaccgc gcccgcgccg tcgccgaaca atacgcaggt tgtgcggtcg ttccaatcga
     6601 cgatgcggct gaaggtttcc gcgccgatga ccagcgcgtt tttcgccatg ccacttttaa
     6661 tgtaggcgtt tgccgtggtc agcgcgtaca taaagccggc gcacaccgcc tgtacgtcaa
     6721 acgcgggaca gccgttggtg atgcccaatt tttgctgcac gatggtggcg gtagaaggaa
     6781 actgcatatc cggcgttgcc gtcgccacga taatcaaatc gatttcgccg ctgtctaatc
     6841 cggctgcatc cagcgcgcgg cgggctgctt cggcggcaag atcgctggtt ttctcatttt
     6901 cggctgcaat atggcggaac ttgatgcccg tgcgcgcggt aatccactcg tcagaggtat
     6961 ctaccttttg ggcaaggtcg tcattgctga cgcggttggc aggaagatag ctgcccgtgc
     7021 cggaaatttt tgcatactgc atgggaacgg cctttttaga gagttggttc agattcggct
     7081 attgtaagcg aaaaaccgat gattgcccaa tacggacagg gtgcaaatga cggaaacagg
     7141 gatgggggtg tggtttggga tacagatggt tttcagatgc agattgccgt ggataggcag
     7201 ggcggggttg ccgatacgct gttttaagcg aaggaaatgc cgtctgaaaa ccaaacatgc
     7261 ttcagacggc attttgattg ttggttttcc atcaaattat tggtgccggt gtttgttttt
     7321 gtatcataaa ttcagaaatt atattttgaa ttgtagaaat atttttagag atatgctcaa
     7381 tagggtagaa gtctccccaa atttccattg tatttccact ataattttct gttttaaggt
     7441 ttttgacttc aatatcccca tcttctaaaa ttgtttgaat cattggcaaa taacatcctt
     7501 cttcaaaata gatagttaat tcggtattgc caaatttggg ttcaggaata attctgaaaa
     7561 caaggacgcc ggcttttttc tcaaaatttg atacggaaaa aaagtgattg atttccctaa
     7621 tgccaggatt ttccaaaacg gggatttgga ttcgctccct gttgtctgcc ataaaaaatc
     7681 cgccaaaaac gaccgtcgat gttttcattc ttatcaaatc attggcttta ttggttaatc
     7741 ggacgtgctt tctatctcaa attccttgtt gataaaagaa atttcccggg attcttgttc
     7801 cgtcagccgg acatcgaaat cttcgatata agtacacagg atttcaaaag ccaaaaaacg
     7861 ttccgaataa tgaatataat ccaatgcgga ttcaatcaga cgggcatcaa gcctgccttc
     7921 aagggatttt ccgaacttca tcatccgttt gtctagagct ttcatccgtt tttctaatgt
     7981 cttcattttt caaatattcc ttattttaca gggaagtatt tgggaagtgc cggacaggtg
     8041 catcgccgtc cgcgcccgca ggttcggggc gggcggatgc cgtctgaaag gggcgggggc
     8101 gttcagacgg catcgtgtgt gttacagacc gccgacattt tcgttttgga cggctttggc
     8161 agtttcgagt gcggcgagtt gttcggcaac gccttgttcg attttggaaa ggccggcgga
     8221 cttggcttcg tggtaggctt cttcgagtgc gtagcggaaa ccggtttcgt ctgtgccgcc
     8281 gtggcttttg atgacgatgc cgcgcagccc gagcaggatg gccccgttga atttgcgcgg
     8341 atcgagtttg tttttcagcc ctttgagggc gggtagggcg gcaacggcgg caagtttgtt
     8401 gaacaggttg ctttggaatt cgcggcggat ggctccgctc atgaatttga ccgcgccttc
     8461 gatggttttg agcatgacgt tgccgacaaa gccgtcggcg acgacgacat ctgcttcgcc
     8521 gtacaggatg ccgttgcttt cgatgttgcc gataaagttg agtttgctgt tttgcagcag
     8581 tttgtaggtt tgtttgacgg tgtccgtacc tttgatgtct tccgtgccga cgttgaccag
     8641 cccgacgcgc ggctgtcctt tttgaggatg gagtgcgtgg acgagttcgc tgccgataac
     8701 ggcaaattgg gcgagctgtt cgggcgtgca gtcgacgttc gcgccaaggt cgagggcgag
     8761 ggtaacatgg tcggtgtcgg acgggaggaa tttggcgatg gcggggcgtt cgataccggg
     8821 tatggtcttg aggacgaaac gtgcggttgc catgagtgcg cccgtgttgc ctgcggatac
     8881 ggcggcttgg gctttgcctt ctttaacttg gttgatggca acgcgcatgg aggagtcttt
     8941 tttgttcttt agggcggact gaggggattc atccatgccg acaacttggg tggtatggca
     9001 gatgtcgatg cgttccatcg gtgcgcctgc cgcggtcagg gcttggcgca gttgcgtttc
     9061 gtcgccggtc ataatcaggc ggacatcggg gtgtgcttgg aggaatgcgg ttgcgccggg
     9121 tacggtaacg gcaagtcctt ggtcgccgcc catggcatct acggcaagtg taatcatagg
     9181 gttctccggt aggttttcgg tctgcggcag gggtcgtgcg ggcttcagac ggcatcggtt
     9241 cggaaaatat gccgtctgaa agggtcagcc tcggccggcc tcggcaaggc ggttgtgttc
     9301 gtcgatgtcg agtgcgtccc acggatagtt gatccaccag tcttctacgg tgatgccgct
     9361 gaaataggga atgccttcgg ggattttgcc tgctttggct ttgatttttt cgtgcagtac
     9421 ggcgacgccg atcgtaccga agtcttcttt gagcagctct gtcaggcaaa attccatggt
     9481 tacgcggctg tcgtccactt catcgacaac aaggacgttt ttgccccgca gggcttcggg
     9541 aacggggtcg agccattgga cttttttgac ttcttctgta acctgtcctt cgttgtcgct
     9601 gtcgtaatag gcggtggtta cggcataaat cggaatttcc agaaaacagc gcagcatacg
     9661 tgccggaata aagccgccgc cgccgatggc aatcatggca tcgtatttga tgtcggcgtt
     9721 ccggattttt tctgccaatg ctttgatgac gcggtggatg tcatcgtagg tgtaccagat
     9781 tttctgtttc atcggaattt ccttggtggt tggggctttt ggaatatttt cacggggtat
     9841 tatacggaac gctttgcccc tgtgtgttga cgtatggcga aagtttggac ggatggtgcg
     9901 tccaatgcaa aaaaagccag taattgcatg gcaatgttct ggctttttca gtcagtcgaa
     9961 tagggattat tcgcctttgg ctttgaccac tttgcggccg cggtacatac cgttggggga
    10021 gatgtggtgc gggcggtgta cttcgccggt tgtgctgtcg acagacagtg caggcgcggt
    10081 cagcgtgtcg tgcgaacggt gcataccgcg tttggaaggg gattttttgt tttgttgaac
    10141 ggccatttca agctcctaga taaataaact gtgtcctaat caattgcttt tcaaacctgc
    10201 caaaacagca aaggggttgg gtttgtcccg attgacttct tccagtgtcc cattgtcgcc
    10261 gcagtcttcg tgtcgcggcg aaaagggcag ggacatcagg atttggtctt ctaccaatgt
    10321 gcgcacgtcg agttcttttt caatcagtat gccttcgagt tcttcgtcgg caagcatgga
    10381 ttcgtccaag gactcttcgt tggaaaacag gacgatacgg ctgctttcat caagcatgaa
    10441 cggcatgggt ttgatacatc tctggcaaat caggggcata tcggctttga cgttcaggtc
    10501 gaggaacagg cgttgcagcc ggtcgcgacc gccggtcagt gtaaacgata ttttggtctg
    10561 cctgtcggcg ggataatcgt gcgaactgac gcgttcatcc aattcttcca gcagaaaact
    10621 gccttgcaga ttctgccctt cggcggcgaa aatttccggg tcaatcaaat tagggtctga
    10681 cataaacggg gtatgatata atctagatgt tctaacgtca atatttttaa gaaaaatgtt
    10741 gcggtacgcc gaaggcgggt atccgtgttt cggcgcatgg ctggatttta ccgccccttc
    10801 gggcgtgttt tcaacggtaa ggaggatggg atgggtttgg aactgccttt gattttgggt
    10861 acgagttcgg ttttccgccg cgaacagatg gaaaggctcg gcattgcctt tcaggcggcg
    10921 tcccccgatt ttgacgaaac gccgatgttg ggggaatccg cccctcagac ggcattgcgc
    10981 cttgccgagg gtaaggcgcg gtcgttgacc gggcgtttcc caaaggcgtt gattgtcggt
    11041 gcggaccagg tggcgtggtg cgacggcagg cagtggggca agccgatgaa ccttgctaat
    11101 gcgcaaaaga tgttgatgca tttgagcggc agggagattg agttttacag cgcggtcgtt
    11161 ctgctaaata cggttacggg caggatgcag cggcatatcg ataagacggt ggtcgtgatg
    11221 aggcagttgg acgagttgca catcctccgc tatttggagc gcgagcctga tgcggtttat
    11281 tgttcgtgtg cgctgaagag tgaggatttg ggtgcattgt tgattgaaag gattgagagt
    11341 accgatccga atgctttaat cggtttgccg gttttccgtc tggtcgattt tttgaaaaat
    11401 gagggcgtgg atgttttgta actgcccgtt tatgccgtct gaagggttca gacggcattt
    11461 ttaaccgatg agtgagggaa tgatgtctcc tgttttgtat ttgattccta cgcctttggg
    11521 tacgcccgat acgccgtgcc tgttgccgca tgaacaacag gcgattgtcg ggctgacgga
    11581 ttttgtcgtg gaagcggaaa aaacggcgcg cgcgcatttg aaacatttgg gcgtgacaat
    11641 gcctatccgc gaactgaatt tgcaaacgct gaacgaacac acggatttga agactttgct
    11701 ggaattgctg aaacctttgc aagaagggcg cagtatgggc attgtcagcg aggcgggttg
    11761 cccggctgtt gccgatccgg gtgcgaatct ggtggctttg gcgcataaac acggttttga
    11821 agtgcgtccg ctggtcgggc cttccagcct gctgctggct ttgatggctt cgggtgcgaa
    11881 cgggcagagt tttgcgttta agggttatct gccgtctgaa aaaaacgagc ggattcagag
    11941 tttgaaggct ttggagcaac gttcgcgcca gcagaatgaa acgcagctgt ttatcgaaac
    12001 gccgtatcgc aacgatgtgt tgttggcgga tgcggtggaa aacctgcatt ctgaaacgcg
    12061 tttgtgtacg gctacggatt tgacgttgcc gactcaggaa atcatcagcc agacggttgt
    12121 gcagtggcga aaaagaaaag aaatgcccaa tctgaaaaaa cgcccgacga tttttgtgat
    12181 gtatgcaggt tgaagatttc cgccccgata gggggtgaac aataaaaatg ccgtctgaac
    12241 aggtttcaga cggcattttt catgctgcgg catttaggaa acgacttcgc cttgggcgcg
    12301 ttgtttttcg atgctgcggt tgatgtgcca ttgctgggcg atggtcagga ggttgttgac
    12361 cacccagtac aataccagac cggcagggaa gaagaagaac atgacggaga aaaccaaagg
    12421 catgattttc atcattttcg cctgcatcgg gtcggtcggc ggcgggttca gataggtttg
    12481 ggcgaacatc gttgccgcca taatgatggg caggatgtag taggggtcgg cgcggctgag
    12541 gtcggtaatc caacccagcc aaggtgcctg gcgcaattct acggaggcga acaatgccca
    12601 atacaatccg atgaagacgg ggatttgcaa cagcataggc aggcagccgc ccagcgggtt
    12661 gattttctcg tctgtgtaaa gctgcatcat ggcttgttgc tgcgccatac ggtcgtcgcc
    12721 gtatttctct ttgatggctt gcagtttggg cgcggcggca cgcattttcg ccatcgaacg
    12781 gtaagaggcg ttggtcaatg gatacagtac ggctttgacg atgatggtta aaacgataat
    12841 cgcccagccc cagttgccga tgatgttgtg cagttggttc aaaagccaaa agagggggga
    12901 ggcgaaccag tgtactttgc cgtagtcttt ggccagttgc aggttgtcgg cgatgtttgc
    12961 gataacggat gtggtctgtg ggccggcgta gaggttgatg gaggcttcgg atttcgcacc
    13021 gttttggata gcggctaaag gcacgctgac gctggtgctg tacagcttgt cgttgcggcg
    13081 tttgatgtcg atacggcagt cgccagcggc gcaaacgctt tgtccgcctt tgggttggag
    13141 gatccaggtg gacatgaagt ggtgttcaat catgccgagc cagccggtcg gggttttgcg
    13201 gatgtattcg gcctcggatt tgccggattt ggcatcgtcg tccaagtcgg agaagctgac
    13261 tttttggaag ttgccttcag gggtataaac aacagggccg acgtaagagt gggtaaagta
    13321 gccttgaccc tcgggttcgc tgtggtcgcg gacgatgcgg tagtccgcgc tcaggttggc
    13381 ggtttgaccg ctgccgttgg cgatgtcgaa gcggacgttg accagatagc tgcctttggt
    13441 gaaagtataa actttgtcga ttttcagacc gcgtgtttca ggtgcgctca ggcggacttc
    13501 aactttgtcg ccttccaagc tgtactgttt tttcggtgcg ctaaagccga tgccttttag
    13561 aatgttgttg ccctgcgcgt ccaaaagttc ggattgggcg acgtaggtgt attctttgcc
    13621 gtcgccaaac aggatgaacg gtttattttc gtcgccggtt gctttgtatt tgagcagggt
    13681 cagccggcgc aggtcgccgc ttttttcatc aatgacggct tgaaccgtgt cggtcgttac
    13741 ggtaatcggc gttgcgggcg cgagcgcggc ttcggcggaa gcggttacgg cctgttgttg
    13801 tgccgtctgt tggggcgcgg ggacgggctt cggagtgggg aacatctttt cccatccgat
    13861 cataatcacc agtgcgatgg cgaaaaacgc cgtgagtctt ttaaaatcca taagagtttc
    13921 ctgaatggtc ggaaatgccg caggttgcgt gttccgatag acggggaata agggcgggct
    13981 tcatacccga cggtcgtgcc gccagcccga aagttcagac ggcattatat agaaaccgat
    14041 gggaaataaa aggaaagcgt gcaatttgaa tattgcgtcg ggtcagggaa cggggtcgtg
    14101 tccgtgtccg ccgaaagggt ggcagcgtgc aatgcgcttg atggcgagcc ggccgccttt
    14161 gaatgcgccg tattttttga ccgcttcgac cgcgtattgc gaacaggtcg gcgtataacg
    14221 gcagcgcggc ggaatcagcg ggctgatgca atattggtag aaccgtatca gtcccagcag
    14281 gagtttggac aataggaagt tcatcgggat gttccttagt atgggaaacc cgttgccgtc
    14341 tgaaccttgc ctgcagagta ccgttctgat catgcctgtt tcctgcatcc ggttgcgggg
    14401 ttgccgaaca tgagttgtgc cagttccgcc cttgcctgtt ttgcggtagc cctgtcgaat
    14461 ttacggcgga cgcgcacgac gaaatcctgc ggcggcagcc ggtttttgtt caatctgaac
    14521 cagtcgcgga tgacgcgctt catataattt cgttcgttgg cgcgtttggc ggtttttttg
    14581 ccgaccacca gaccgatgcg gggatgatcc agcccgttgc cgtttgaacg cgaaacttgc
    14641 agcaggtcgc ggctgcggcg gtttctgaat gcaaaaacgg atgaaaaatc atccgttttc
    14701 aacaagcggt actgccttcc gaagcggtag tccaaaatta caccgccaag cgtttgcggc
    14761 ctttggcgcg ccgtgcggcc aatactgcgc gtccgccgcg cgttttggag cgcacgagga
    14821 agccgtgggt gcgtttgcgt ttggtaacgg aaggttgata agtgcgtttc atgatgtttc
    14881 ctaaaaatcg gtagatagat aaaccgtgaa ttacactcca atttgccgct tttgtcaatc
    14941 aatatagaaa ctttgcagga ggattgcccg attgcctgcg gatttctgcc gcctttctgt
    15001 ggataaattt ttgcacgggt tgtggataaa atattcgtga gtcggtataa tcgggcagtc
    15061 gcgttttgaa ccgacgcgcg ttcaacagat ttgttttctt tttgaaaata ttatattttc
    15121 tttgttttcg atttcatttt taccgattcg agcctatcca tgacattagc agagttttgg
    15181 ccgctgtgcc tccgccgtct tcacgatatg ttgcctcacg ggcagtttgc gcaatggatt
    15241 gcgcccctta ccgtcggtga ggaggggggc gtatgggtgg tgtacggcaa gaaccagttt
    15301 gcctgcaata tgctcaagag ccagtttgcc ggaaaaatag aagcggtgag ggaagagttg
    15361 gctgccggcc gttccgcctt cgtattcaaa ccgggagaag gcgtgtgtta tgagatggcg
    15421 gcggttgaag gtgctgtcga acctgccgag ccgtccttgc acgcggggtc ggaggagatg
    15481 cctgtgcagg aggttctgtt ggacgagctg ccgtctgaag agcctgtcaa acccgctgcg
    15541 tcgaaaacgg cggcggatat tttggcagaa cgtatgagaa acctgccgca cgagccgcgt
    15601 caggctgccg ggtctgcttc ccggccggaa tcggcggcag ttgccaaagc acggacggac
    15661 gcgcagcgcg atgcggaaga ggcgcgttac gagcagacca atctgtcgcc ggactatacg
    15721 ttcgacacat tggtcgaagg caagggaaac cgccttgcgg cggctgcggc gcaggcgatt
    15781 gcggaaagcc cgggacagag ttacaacccg ttcttcctct atggcagtac gggtttgggt
    15841 aaaacccacc ttgtgcaggc ggtcggcaac gaactgttga aaaaccgtcc cgatgccaaa
    15901 gtgcgctata tgcattcgga cgactacatc cgcagcttta tgaaggcggt tcgcaacaat
    15961 acctatgacg tattcaagca gcaatacaag caatacgacc tgctgattat cgacgatatt
    16021 cagtttatta aaggaaaaga ccgtacgatg gaagagtttt tctatctgta caaccatttc
    16081 cacaacgaga aaaaacagct catcctcact tgcgatgtgt tgcctgcgaa aatcgaaggc
    16141 atggacgacc gcctcaaatc ccgtttttca tggggactga ctttggaact cgagccgccc
    16201 gaattggaaa tgcgcatcgc cattttgcag aaaaaggcgg aagcggcggg catcagtatc
    16261 gaagacgaag ccgcgctgtt cattgccaat ctgatccgtt ccaacgtgcg cgagcttgaa
    16321 ggcgcgttta atcgtgtcgg agcgagcagc cgctttatga accgtcccgt catcgacatc
    16381 gatttggcgc gtaccgcttt gcaggacatt attgccgaga agcacaaagt catcaccgcc
    16441 gatatcatca tcgatgcggt ggcgaaatat taccgcatca aaatcagcga cgtactcggc
    16501 aaaaaacgca cgcgcaacat tgcccgtccg cgtcaggttg ccatgagcct gaccaaagaa
    16561 ctgaccactt tgagcctgcc gtctatcggc gattcgtttg gcggacgcga ccatacgacc
    16621 gttatgcacg gcatcagggc agtggcgaaa ctgcgcgaag aagatccgga attggctcag
    16681 gattatgaaa agctgctgat tctgattcaa aactgatgga aaacgttttc agacgacatg
    16741 atattgccca tgccgtccga agggtaaaga tatagcggat taactttaaa ccagtacggc
    16801 gttgcctcgc cttagctcaa agagaacgat tctctaaggt gctgaagcac caagtgaatc
    16861 ggttccgtac tatttgtact gtctgcggct tcgtcgcctt gtcctgattt aaatttaatc
    16921 cactatacaa accgatttaa aggagcgaac atgttgattt tacaagccga acgcgacagc
    16981 ctgctcaagc cgctgcaagc cgttaccggc atcgtcgaac gccgccacac cctgcccatc
    17041 ctgtccaacg tcctgattga gggaaaaggc ggtcagacca aactcttggc aaccgatttg
    17101 gaaatccaaa tcgacactgc gggtcccgag ggcggcgcgg gcgacttccg catcactacc
    17161 aatgccaaga aatttcagga cattttgcgc gcgctgcctg ccggtgcgct ggtgtcgctg
    17221 gattgggacg acagccgtct gacgctgaag gcgggcaaat cgcgttttgc cctgcagacc
    17281 ctgcctgccg ccgattttcc gatgatgaat gtcggcgagg acatcagcgc gactttctcg
    17341 ctggagcagg agcgtttcaa aaccatgctg tcgcaagtgc agtacagcat ggcggtgcag
    17401 gacatccgct attatctcaa cggtctgctg atgcaggttg agggcagcca gttgcgcctt
    17461 gtggcgaccg acggacaccg ccttgcctat gcagcctgcg cgattgatgc ggatttgccg
    17521 cacgccgaag tgattttgcc gcgcaaaacg gtgctggaac tgttcaaact gttgaacaac
    17581 cccgacgatc cgattcaaat cgagctgctg gacaaacagg tgcgtttcca atgcaacggc
    17641 acgaccatcg tcagcaaggt catcgacggc aaattccccg atttcaaccg cgtgattcct
    17701 ttggacaacg acaagatttt cgttttgtcc cgtgccgaac ttttgggtgc gctggaacgt
    17761 gtgtccattc ttgccaacga aaaattccgt ggcgcgcgcc tgttcctgca atccggcctg
    17821 ttgagcgtcg tgtgcagcaa caacgagcag gaagaagcgc gcgaagaaat cgaaatcgcc
    17881 tatcagggtg gcgaactcga agtcggtttc aatatcggct atctgatgga cgtgttgcgc
    17941 aacatccatt ccgacgatat gcagcttgcc ttcggcgatg ccaaccgctc gacgctgttt
    18001 accgtgccga acaatccgaa tttcaaatat attgtgatgc cgatgcggat ttgacggttt
    18061 tccggaacac gatgcccgta ttggagatat gccccgaacc gtgcagacgg attcggggtt
    18121 ttgttcggct gtcggaaggg taatgccgtc tggaatgcgg cggcttgggg ttgggagcgt
    18181 ataggggaag tgcttgtgca ggaatggcga aggtgtgttt ttgactccgg tccgccctgt
    18241 ttagattgct gtaaaaccat gccgtctgaa acggaaaggt ttcagacggc atggtttttg
    18301 attggggcgg tgcggttcag cctccgagca gtgtccacaa atcgttttgc agatcggctt
    18361 cgctttcgcc ctcggcgggc gcggcgcgga tatagccgcc gtcgggctgc atcagccatg
    18421 cgtgggtgtt gtcgtccagt gccatttcca aaccttcgcg gatgacgcgt tttttgagtt
    18481 cgggcgcggc aatcggcgcg gcggtttcga tgcggcggaa gaagttgcgc cccatccaat
    18541 ccgcgctgga gataaaggta tcgtccgcgc cgttgttgtg gaagcaatac acgcgcgcgt
    18601 gttcgagctg cctgccgatg atggagcgga cgcggatgtt ttcggacaag ccttttacac
    18661 ccgggcgcaa ggtgcacata ccgcgcacaa tcaaatcgat ttgtacgcct gccgcgcttg
    18721 cccgatacag ggcttcgatg acggtcggtt cgatgagcga attcatcttg gcggtaatcc
    18781 gcgccggttt gccggctttt gcgtgttcgg tttcgcgtgc gatgcggtcg ataaccattt
    18841 tgtgcagggt aaacgggctt tggtagagtt tgttcagccg cccgggtttg cccaagcctg
    18901 tgatttccat aaacaatgtg ttcacatcgg cggtgatttg ttcgtcggcg gtaatgatgc
    18961 cgaagtcggt gtagatgcgc gatgtgcctt ggtggtagtt acccgtgccg aggtgggcgt
    19021 aacgcttgag tacgccgtct tcgcggcgga tgatgagtgc cattttggcg tgtactttgt
    19081 agccgaacac gccgtatacg acgtgcgcgc ccgcctcttc gagctgcttc gcccagttga
    19141 cgttgttggc ttcgtcaaaa cgcgccatca gttcgacgac gacggttact tgtttgccgg
    19201 cgagtgccgc cttcatcagg gcgcggacga gttcggagcg cgtgccggtg cggtaaatcg
    19261 tcattttgac ggcaagcacg tcggggtcgg cggcagcttc gcgcatcatt tcgacaacgg
    19321 ggtcgaacga ttggtagggg tggtgcagca ggatgggcga ttggcgcacc aaatcgaata
    19381 tggacgcggt tttgcccaag gctttcagtc tgcccggcgt gtgtgtggga aatttcaaat
    19441 cggggcggtt gactaggtcg gggacggcgt tgaggcgcac gaggttgacc gggcctttga
    19501 cctgatagag ttcggcggcg gtcagtttga attgcgcgag cagaaagtcg cggatgtagg
    19561 cgggacaggt gtcggcgact tcgagccgca cgccgtcgcc gtattcgcgg tcgtgcagtt
    19621 cgttttggat ggcggcgcgg aggttttgca ggtcttcttc gtcaacggtc aagtcgctgt
    19681 cgcgcgtcag gcggaactgg tggcagcctt tgacgttcat gcccgggaag agtttgccga
    19741 cgtgggcgtg gaggatggac gacaggaaga cgaagccgtg tccgccgcca cacagttcgg
    19801 acggcagggg aacgacgcgc ggcaggatgc gcggtgcctg cacaatcgcc atccccgaag
    19861 gtctgccgaa tgcgtccgtg ccgtcgagtt cgacggcgaa gttgagcgat ttgttcagcg
    19921 gtcgcgggaa ggggtgggac gggtcgagtc cgatgggggt cagaatcggc agcaattcgc
    19981 ggtcgaaata gctttcgatc cactttttct gcgcgcccgt ccagttgcgg cggcggtaaa
    20041 aatggatgcc ttcttgtgcc agctcgggct gaaggacgtt gttgaacagg tcgtactggt
    20101 gccgtatcag ggagcgcgcc gcttcggtaa cgtcggcgat ggtttcagac ggcattttgc
    20161 cgttgtccag cctgcgccgg gggtgcagct tgttttcacg cttgagccac gccatgcgga
    20221 cttcaaaaaa ctcgtcaaga ttggacgaca cgatgcacag aaagcgcagg cgttccaaaa
    20281 gggggacgtt tctgtcttcc gcctgtgcca acacgcggcg gttgaatgcc agcaggctca
    20341 gttcgcggca gaggatgcgg ttttgttcgg gcataaggtt ctcccaaaga tggttgttgt
    20401 tcggtcggag cgggcaggca atgccgtctg aaagcttggc ggcatctgcg ccggtttcag
    20461 acggcatgac gtggcggaca gtctgtttca ggcatcgggg caatgtgaaa acagataccg
    20521 gcgcgtgcgg tatccgtctg tttcaaatca cttccaacac aaaataatga cgcagttttt
    20581 cgtacaccgc atcgctgacg ttgatgcagc cgttggtcat aatcctgtcg gacacaaccg
    20641 aagatgcgat acgttcgctc cgtctttccg acggtatctg attccaaacg cggtgcaggg
    20701 cgaaaagaaa atcaccctcc tgcttgaaac cgatgacttc gccgccgtat ccgggcttgt
    20761 cggtactgtt cagcgtcagc ccgaacgtcc ccttgggcgt ggcaacaccg atgaggacgg
    20821 ggtggcactg cctgtcatcg gcaaaacaga gttccgcttt ggatgtgttt acgatgactt
    20881 ttttcttttg aatataagcg ttgaccgcat ccggttgccc ctgtgcatat acgggggcaa
    20941 ccattgacag gccgcataat atccctaaaa acaggcggaa cggcatgtcg gattattggc
    21001 ggatacgttt cggttttgcc ggcacgattt cgcggatgat gacttgcggc tcggctttgg
    21061 gtgcgggcgg cggacaggcg gcatctttcg ggaagacggg gttccagtag aagctgcggg
    21121 caaatttgtc tttatcgaaa atcactttgt attggcaggt agtaacatct tctacgccgg
    21181 aagtgttttc agggtcgata cctacgcccg gggtatggaa gtggaacagg taatcccatt
    21241 cgcgcacgcc gtacatacct tcgtcgtaat gcgggcggcc caggattttg tagatgtcgt
    21301 ctttggtcag gccggggcgc atctgatcca gttcgtcata agtcgggaat gtgccgcgct
    21361 tgttgtcgag cgttacggaa tagggtttcg ggaaaaccgg attgtcggtc gtgccgtcgg
    21421 ctttgacgtt gcttttggtt gcgcaggcag acagaacgcc cgccgccaat actgccaagc
    21481 ccggtttgac gatttgtttg attttcatat gcagaatcct cttttacccg atgcccgtct
    21541 gccttttgtt cggaagagcc gcatcaggag agttttaaac atttgatttt ggtttgtaat
    21601 attagcataa aaaacgtgcg tatcaataaa cgcggtgtat ttgtacggca tacggaatga
    21661 tgcgcgtgcg aatttacgca tcctgtcggc aatttgccga ttcgccgaca tcggcaacct
    21721 gttataattc ctcctttaaa ttcctaacgt tttcaagcga aaaacagaat gaccatgcaa
    21781 gaacattacc aacccgccgc catcgagcct gcggcgcaga aaaaatggga cgacgcccgt
    21841 attttcaacg tctccgaaga cgcttccaaa cccaaatact actgcctctc catgttcccc
    21901 taccccagcg gcaagctgca catggggcat gtgcgcaact acaccatcgg cgacgtattg
    21961 agccgcttca aacgcttaaa cggcttcaac gtcatgcagc ctatgggttg ggacgcgttc
    22021 ggtatgcctg cggaaaatgc ggcgatgaaa aacaacgtcg cccccgccgc ttggacgtac
    22081 gacaacatcg aatacatgaa aacccagctc aaaagcctgg gttttgcgat tgactgggcg
    22141 cgcgaaaccg caacctgcaa acccgaatac taccgctggg aacaatggct gtttaccaag
    22201 ctgtttgaaa aaggcatcgt ctatcgcaaa aacggcacgg taaactggga ccctgtcgac
    22261 caaaccgtcc ttgccaacga gcaagtcatc gacgggcgcg gctggcgttc gggcgcgttg
    22321 atcgaaaaac gcgaaatccc gatgtattac ttcaaaatca cggattacgc cgaagagttg
    22381 ctcaacgatt tggacaagct ggaacactgg ccggaacaag tcaaaaccat gcagcgcaac
    22441 tggatcggca aatcgcgcgg catgaccgtg cgcttcgccg tttccgacga cagcaaacag
    22501 ggcttggaag gcgattacgc gaaattcctg caagtttata ccacccgcca cgacacgctg
    22561 atgggcgcga cttatgtcgc cgttgccgcc gagcatccgc tggcaaccgc cgcagccgcc
    22621 gacaaacctg aattgcaggc atttatcgcc gagtgcaaag ccggctcggt tgccgaagcc
    22681 gatatggcga cgatggagaa aaaaggcgtg ccgaccggcc gctacgtcgt caacccgctc
    22741 aacggcgaca agctggaagt gtggattgcc aactatgtat tgtggggcta cggtgacggc
    22801 gcggtcatgg cggttccggc gcacgacgaa cgcgatttcg agttcgccgc caaatacagc
    22861 ctgccgaaaa aacaagtcat tgccgtcggc gacaacgcat tcgacgcaaa ccgatggcaa
    22921 gagtggtacg gcgacaaaga aaacggcgta ttggtcaaca gcggcgactt ggacggcttg
    22981 gattttcaga cggcattcga tgccgttgcc gccaagctgc aaagccaagg cgcgggcgaa
    23041 ccgaaaaccc aataccgcct gcgcgactgg ggcatttcgc gccaacgcta ctggggctgc
    23101 ccgattccca tcgtccattg cgaacaatgc ggcgacgtac ccgtcccagc cgaccaactg
    23161 cccgtcgttt tgcctgaaaa cgtcgtaccc gacggcatgg gttcgccttt ggcaaaaatg
    23221 cccgagtttt acgaaaccgc ctgcccatca tgcggcggcg cggcgaaacg cgaaaccgac
    23281 accatggaca ccttcatgga atcgagctgg tacttcttcc gctacatgtc gcccaagttt
    23341 tcagacggca tggtatcggc tgaagccgca aaatactggg gcgcggtcga ccaatacatc
    23401 ggcggcatcg aacacgcgat tttgcacctc ttgtacgcgc gtttcttcac caaactgatg
    23461 cgcgacgaag gtctagtcaa tgttgacgaa ccgtttgaac gcctgctgac gcaaggcatg
    23521 gtcgtttgcg aaacctacta ccgcgaaaac gacaaaggcg gcaaagattg gatcaacccc
    23581 gccgatgtcg agctgacgtt cgacgacaaa ggccgcccca tttccgccgt cctcaaagcc
    23641 gacgggctgc ccgtcgtcat cagcggcacg gaaaaaatgt ccaagtccaa aaacaacggc
    23701 gtcgatccgc aagaactgat taacgcctac ggcgcggaca ccgcccgcct gtttatgatg
    23761 ttcgccgcac cgcccgaaca gtccctcgaa tggagcgaca gcggcgtcga aggcgcacac
    23821 cgcttcctgc gccgcctgtg gcgtaccgtt tacgaatact tgaagcaagg cgaagcggtc
    23881 aaagcgttcg caggcagcca agacggtttg tctaaagaac tcaaagacct gcgccacaaa
    23941 ctgcacgcga ccactgccaa agtcagcgac gactacggcc gccgccagca gttcaacacc
    24001 gccatcgccg ccgtgatgga attgctcaac caatacgaca aaaccgacac cggcggcgaa
    24061 caaggacgcg ccgtcgccca agaagtattg gaaaccgccg tacgcctgtt gtggcccatc
    24121 gtgccgcaca tctgcgaaac cctgtggagc gaattgaacg gcgcgaaact gtgggaagca
    24181 ggctggccga cagtcgacga agccgccttg gtcaaatccg aaatcgaagt gatggttcaa
    24241 gtcaacggca aactgcgcgg caaaatcacc gtcgccgccg acgcctccaa agccgacctc
    24301 gaagccgccg cccttgccac tgaaggcgcg gtgaaattca tggaaggcaa gcccgcgaag
    24361 aaaatcatcg tcgtaccggg cagactggtg aacatcgccg tctaaaccgc tcttaaggtt
    24421 tagccacacg gataaaggcc gtctgaaact tggaaacagg gtttcagacg gcctttttaa
    24481 ggcagattgg gttgtctgca ttacagacct caaatatagt ggattaactt taaaccggta
    24541 cgacgttgcc ccgccttgcc ctgctgtctg cggcttcgtc gccttgtcct gatttttgtt
    24601 aatccactaa aactgaaatc tcaaaatcca aaacatgaat ttatttttcg ataccaaatt
    24661 gggaaagcaa caaaataaag caacccataa aatccgtgta atgagtgagg cttggctgga
    24721 aaaaaacggc tactgcccct gttgcggaag caagccgatg cagagatttg ccaataacaa
    24781 acctgttgca gacctctttt gctcaaattg ccacgagcaa tatgaattaa agagtaaaaa
    24841 tcaaaaaacc ataggtaaca gtgtgcctga cggtgcatat cgcactatgt tggagcgcat
    24901 ccggtcagat accaacccca actttttctt tcttgcatat aaaaaagcgg attactctat
    24961 acggcaattg gtgcttgtac ccaaacattt catcacgccg gacatgatta ttcccagaaa
    25021 taaaggcatt aaaaaccggc cgaaccacat tatgtgttcc atcaatctcg cccctttgcc
    25081 tgaaagcgga aaaatattct taatagacga ttctcgcatt atcgaacccg aaaccgttct
    25141 gaaaaaatgg caatccaatc tgtttttacg caaccaaaat gcggagcgca aaggctggct
    25201 tttggctgtt atgaaatgta tcgaccaact ccccgaagaa ttcacattgt cgcaaatgta
    25261 tgaatttgaa aacaaactat ccatccaatt tccccaaaac aagcatatta aagacaaaat
    25321 ccgccaacag ttgcaaattt tgcgcgacca aaatacgatc gaattcattg gtcgcggact
    25381 ttacaaaaaa atcgacaaat tgcccccgac ttccaaggcg ttttaatttc aaatcatgat
    25441 actgaattca caagggatat ttattcttct gctattctga aaacagttga agtacagcgc
    25501 aatattgccg caaaaggcag cagccgtaaa aaagtcgggg aattattggc aatttatgcg
    25561 taatttcgac aaattggctg aagaagctgc cgaacacgcc gctgaaggcg cggtgaaatt
    25621 catagacggc aagcctgcca agaaaatcat cgccgtaccg ggcagactgg cgaacatcgt
    25681 ggtgtaaatc tttttaaaaa catcaaacaa tgttaagatg ccgtccgaaa tccgttttag
    25741 attcagacgg cattttttaa atttaatttt ttaaggggta agcctaatat gaaatttcct
    25801 tccttccttc cttccttcct ttaaacggat actctgcctg tcggcagtaa tctcggtatt
    25861 gggggcttgt acggtcgttg ctgatgttta cggtcaggat tccgccacaa tgaacgctgc
    25921 ggctgccaaa gattatatga aaacggttga gttgaacaag tctgccggca atgtcgatac
    25981 cacatccaga acagcccgca gggtgcaggc agtatttcga cgtatgctgc cttatgccga
    26041 tgcggcaaat aataccggcc ataggtttga ctggaaaatg acggttttca aaaacgatga
    26101 gctgaacgcg tgggcgatgc ccggtggaaa aatggcgttt tatacgggga tagtcgacaa
    26161 actcaagctg accgatgacg aaattgccgc cattatgggg catgaaatga cgcacgccct
    26221 gcatgaacac ggtaaaaata aggtcgggca gcaaatcttg accaatacgg cggcgcagat
    26281 aggcacgcag attatattag acaaaaaacc ggatactaat ccggaattgg tcggattggg
    26341 tatggatatt ttggggacgt acggtcttac cttgccttat agccgcagct tggaagaaga
    26401 agccgatgag gggggaatga tgttgatggc gcaggcaggc tatcatccgg cggccgctgt
    26461 cagggtttgg gaaaaaatga atcaggaaaa cgaccaaaac ggctttattt atgctattac
    26521 ctctactcat ccgacaaaca atgcccgtat agaaaatcta aaacggttgt tgccgaccgt
    26581 tatgccggtt tatgagcaaa gtgtcagaaa taaggggcgc gttaataaaa aacgtcggcg
    26641 ttaagggaag gggatcgaga atttgagcca ttgtttcaaa atgccgtctg aaatcgtttg
    26701 agattcagac tgcatttaaa cagttctgtg ccccacccgt tttgccttca agcccgcgcc
    26761 gcttaaatcc aatccccccc tgcaacgggc gcaaaaaatc cccgtacctc gcggtcggtg
    26821 atgtttcggg tttcgcgccc gtctgcgccg tgccttgctg tcgggctatg tcgtctgcac
    26881 cgccgccgtt tcggcttggt ttgatgcggt cgggcattta tgcggctacg ccctgatatt
    26941 cagttcggcg gtcttgcctg tcttggcagg catattcagc agcgcgtcta ttgtgaactt
    27001 gctgattttg ccgtttgcca agtcggctac gcaatggctt cgatgccctt gtttttgacg
    27061 atggtcagca gcttcaacgc cgcgccgctc ggctttttaa cgccccgctc ccaagccgaa
    27121 acgtgatttt ttcccacgtt gagatagatg gcgaaagcgg cttgcgatag tgcctccttc
    27181 tccctgattg ccttgatgtc cccgccgctc aacggtttga tttcggtcag gcaggacttg
    27241 tcaaagccgc gcatagtctt tttgtcgatt gcgccgatgt tgtaagcccc ttccatcatt
    27301 tcatgaatgg cggctaatgc ctcgcttttg tatttcatga ttctatctcc gtgataatgc
    27361 cgtcttcttt caaagccgcc agctctgccc gcgtgtattt cagataatat gcggcggctt
    27421 ttcggtaaac gtccagttct ttatccgaaa tgttttccct gtcgttcttg gcaaaggcgt
    27481 aaacgaaaaa tgccctatct gcctgtttga acaatatcag gctgcgatag ccgccgcttc
    27541 tgccttgtcc ttgccttgct atgcgctgct taatcacacc gccgcctaaa tctgcgtcta
    27601 tcagcccgtt atccgcccgc tctaccgctt ccagcagctc ggaatcgttg attttgtgct
    27661 tcttggcaaa tttcactatc cattggtttt tgaatatccg cattgtgtag ccgttgatta
    27721 gtgatacttg gaattatacc agagacaggc aaggcggcaa gtcgcctttt tataccctgc
    27781 aatttctccg tttatgccgc ctgaaacacc ggcggctaac ctgattattt tttaatcaat
    27841 cgcttttaat ggctcaaatt gcgtttttag cgcgtttcgc tgtcttccta tgccagtata
    27901 taaccaagcg gtttaaccgc cattcctgct caaaataggc gcggttccgt gcgttttggc
    27961 ttttcattcc gctacgctcc cactgccaag acggtgaaaa accggaaaac cccaaaagtt
    28021 gttaccgatt cgttaccatt tgttaccgca tttgttacca attttttatc tttttcttat
    28081 tttatagtgg attaaattta aaccggtacg gcaaggcgag gcaacgccgt accggtttaa
    28141 atttaatcca ctatagtata ggttttttcc aaaaacccaa aaaaaaccgc tctgcatctt
    28201 ggacggtttc cccctcattc cctacggtgc gtcagtattc cccgcaagct atttcccgct
    28261 tggcattcct gatttggtgg tcgttcagct ttatttttgc ggtatttttt cgaggtattt
    28321 ttcagatacc gcaaaagata ccgtaaattt taggttactt caattaggct gaattaaacg
    28381 gaattataca atcaggaaga agacaatagg atagcaacca gtctgaaagc cttgtattga
    28441 ttgggattgg ttgacggcat tagacaggat taaacgaaaa aaacgcttag aatttctaag
    28501 cgttttgtgt gttggtgccg acagcgagat ttgaactcgc acagcctacg gccactaccc
    28561 cctcaagata gcgtgtctac caatttcacc atgtcggcat ttgaaaaact gttatttctg
    28621 ctgctgagga acaggggcag aaggttcggt attgcttacg ggtttgggtg cttgctgagt
    28681 ttgttgtacg ttgctgaagt ccaaaccgtg ttttgttgtg tgggtgtgaa tatacaccat
    28741 agccatgcag gttgcaaaga aaaatgttgc tgcaacggcg gtcgagcggc tgaggaagtt
    28801 agcgttgccg gcagagccga atacgccttg cgcgctgccg cttcccgatc cgaaagtcgc
    28861 gccggcatcc gcgcctttgc cgtgttggag caatactaac acgatgacgg ccaaagcgga
    28921 aattatatta acaatccaaa ttagggtttt gaaggcttcc atatttttct acgcattttg
    28981 tgcggcactg atgatggcgg taaaggagtc gtacgacaat gacgcgccgc cgacgagtgc
    29041 gccgtccaca taaggtactg cgaagatgtc ggccgcgttg tccgctttca cacttccgcc
    29101 gtaaaggacg cggattttaa catcgcttcc gcacaaagac aagatttctt tgtagatgaa
    29161 tgcgtgcata tcggcaatct gttcgacggt ggcgactttg ccggtgccga ttgcccagac
    29221 gggttcgtag gcgacggcga tgtttttggt gtccagtcct tggaggatgg aaagctgatg
    29281 ggcgatgact tcgtgttctt tgccggcttc gcgctcttcg aggctttcgc cgacgcacaa
    29341 taacgggatg agtccgacgt tgaggacgtt ttccattttg cggcgttgga tttcgttttt
    29401 ttcgccgaaa taaaggctgc gttcggagtg tccgatgagg acgatgtctg tgccggtgtc
    29461 ggcgagcatt tcggcggaca cttcgccggt gtacgcgccg ttgtcgggga agcggctcac
    29521 gtcttgggcg caggtgagga tgcggttgtt taagacgatt tgcatggcgt tgtgcagttg
    29581 cagcaggtaa acggtcgggg cggcgagtcc gatgaggacg cgttcggcgg tggggaggat
    29641 gcggaagcgg tgcatcagtg cgttgttgtt ttggagccgg ccgttcattt tccagttgcc
    29701 gatgacccat ttttgatccc acattccgat ttggcgatac atcttttttg ctccgtgtcg
    29761 tgttttttct gtctgccgcg tgtggcgcgt tgcaatgtga agtttagtgg atatgcggcg
    29821 ggttcgcaac ttggggcggg cggctgcggg ggcggttcgg aatgttgttt cgggcaggtt
    29881 gttttataat ggccgcctga tatgtatgca actataggag atgtgatgca cgcgcttcat
    29941 ttttcggctt cggacaaggc cgcgctttat cgggaggtgt tgccgcagat tgagtctgtg
    30001 gtggctgacg aggcggattg ggtggcgaat ttggcgaaca cggcggcggt tttgaaggag
    30061 gcgttcggtt ggttttgggt gggtttttat ttggtcgata cgcgttcgga cgaattggtt
    30121 ttggcaccgt ttcaggggcc tttggcgtgt acgcggattc cgttcggtcg cggggtgtgc
    30181 ggtcaggctt gggcgaaggg agagacggtg gtcgtcgggg atgtcaacgc gtatcccgac
    30241 catattgcct gttcgtcttt gtcgcgttcg gagattgtcg tgccgctgtt ttcagacggc
    30301 cgctgtatcg gcgtgttgga cgcggacagc gaatatttgg cgcagtttga tgagacagat
    30361 gctttgtatt tgggcgagct ggcgaagatt ttggagaagc ggtttgaggc ttcgcgtcag
    30421 gcggtttgag tgagggaaag gggcgggcgg cgcgtgccga agttggcgcg gcgggagtgt
    30481 ggttttataa tgcctgctat tgttggaaca attatttgac ggagcactaa atggattttg
    30541 aaaaagcgcg gttcaatatg gtcgaacagc aaatccgtcc ttgggatgta ttggattttg
    30601 acgttttgga cgctttggcg gagattccgc gcgagctttt tgtcgatgag gacttgcagg
    30661 gtttggcgta tgcggatatg gcgctgccgc ttgccaacgg gcataagatg ctcgagccga
    30721 aagttgtggc gcggctggcg cagggcttga agctgacgaa aaacgatacg gttttggaaa
    30781 tcggcacggg atcgggctat gcggccgcgc tgttggcgaa actggctggt cgtgtggttt
    30841 cggacgacat cgatgccgaa cagcaaaagc gcgccaaagc ggttttggac ggcttgtctt
    30901 tggaaaacat tgattatgtg caaaacaacg ggctgaccga actttccgca ggcgcgccgt
    30961 ttgatgcggt ttatgtcggc ggcgcggtaa accttgtgcc tgaagtgttg aaagaacagt
    31021 tgaaagatgg cggacgcatg gcggttatcg taggccgcaa gcctgttcag cgcgcgcttt
    31081 tgattacgcg caagggcgat gtgtttgaag agaaggtgct gttcgatacc ttggtggcgc
    31141 atttggatga taaggatgcc gatccgtttg gcggtttcga tttttaagat gttgacaggt
    31201 catgccgtct gaaagcgggt ttggggcttc agacggcatt ttgcttggtt tttttggggg
    31261 ggtgcgatgg atattgtgca acttgggccc gcggaattga aagcgtggat ggacgaaggg
    31321 cggatgtttt gtttgttgga cgtgcgtacg gatgaagaaa cggcggtttg ttcgctgcca
    31381 aatgcgctgc atatcccgat gaatctgatt ccgctgcggc agaacgagct gccggatgat
    31441 gtgccgcttg tggtgtattg ccatcacggt atccgcagtc tgcatacggc gatgtatttg
    31501 gcggacgcgg gttttgaaaa cctgtacaac cttcagggcg gcatcgatgc atgggcggcc
    31561 gagattgacg gcaacatgat gcgttattga ggcaggcatt tcagacggca ttccttaaat
    31621 gcgtgtacct gtggtgttcc aaaaggtcgt ctgaaagtgc agcttctgcg aagctaaagc
    31681 gcggtatcaa cgaagttaaa atttgcctga accttaaagg cagcctgcac cccaatcccc
    31741 tctccccgtg ggagagggct aggacgaggc tggcaaactg aaagtttgct tgggcggcat
    31801 tttcaatgtg caggctg
//
LOCUS       DAWHSK010000003        30471 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.003, whole genome shotgun sequence.
ACCESSION   DAWHSK010000003 DAWHSK010000000
VERSION     DAWHSK010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 30471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30471
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.003"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            32..898
                     /locus_tag="WIC03_000066"
     CDS             32..898
                     /locus_tag="WIC03_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter six-transmembrane
                     domain-containing protein"
                     /protein_id="HEZ3882645.1"
                     /translation="MWKMLKHIAQTHRKRLIGTFSLVGLENLLMLVYPVFGGRAINAV
                     IAGGVWQALLYALVVLLMWLVGAARRIADTRTFTRIYTEIAVPVVLEQRQRQVPHSAV
                     TARVALSREFVSFFEEHLPIAATSVVSIFGACIMLLVLEFWVGVSAVGILALFLWLLP
                     RFAAISENLYFRLKNSLECDNHFIRKGDRRQLDRHYGLVARLRVLISNREAFGYLCVG
                     AAMGILFGFAFVMMTLKGYSSAGHVYSVGTYLWMFAMSLDDVPRLVEQYSNLKDIGQR
                     IEWSERNIKAGT"
     gene            complement(949..2001)
                     /gene="sohB"
                     /locus_tag="WIC03_000067"
     CDS             complement(949..2001)
                     /gene="sohB"
                     /locus_tag="WIC03_000067"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HEZ3882646.1"
                     /translation="MWKEILLNYGIFLLELLTVFGAIALIVLAIVQSKKQSESGSVVL
                     TDFSENYKKQRQSFEAFFLSGEEAKHQEKEEKKKEKAEAKAEKKRLKEGGEKSSETQK
                     SRLFVLDFDGDLYAHAVESLRHEITAVLLIAKPEDEVLLRLESPGGVVHGYGLAASQL
                     RRLRERNIPLTVAVDKVAASGGYMMACVADKIVSAPFAIVGSVGVVAEVPNIHRLLKK
                     HDIDVDVMTAGEFKRTVTFMGENTEKGKQKFRQELEETHQLFKQFVSENRPQLDIEEV
                     ATGEHWFGRQALALNLIDEISTSDDLLLKAFENKQVIEVKYQEKQSLIQRIGLQAEAS
                     VEKLFAKLVNRRADVM"
     gene            complement(2105..2194)
                     /locus_tag="WIC03_000068"
     tRNA            complement(2105..2194)
                     /locus_tag="WIC03_000068"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2158..2160),aa:Leu,seq:taa)
     gene            complement(2297..2370)
                     /locus_tag="WIC03_000069"
     tRNA            complement(2297..2370)
                     /locus_tag="WIC03_000069"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2336..2338),aa:Cys,seq:gca)
     gene            complement(2397..2472)
                     /locus_tag="WIC03_000070"
     tRNA            complement(2397..2472)
                     /locus_tag="WIC03_000070"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2437..2439),aa:Gly,seq:gcc)
     gene            complement(2494..2569)
                     /locus_tag="WIC03_000071"
     tRNA            complement(2494..2569)
                     /locus_tag="WIC03_000071"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2534..2536),aa:Gly,seq:gcc)
     gene            complement(2593..2668)
                     /locus_tag="WIC03_000072"
     tRNA            complement(2593..2668)
                     /locus_tag="WIC03_000072"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2633..2635),aa:Gly,seq:gcc)
     gene            complement(2694..2769)
                     /locus_tag="WIC03_000073"
     tRNA            complement(2694..2769)
                     /locus_tag="WIC03_000073"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2734..2736),aa:Gly,seq:gcc)
     gene            complement(2849..3412)
                     /gene="pgsA"
                     /locus_tag="WIC03_000074"
     CDS             complement(2849..3412)
                     /gene="pgsA"
                     /locus_tag="WIC03_000074"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEZ3882647.1"
                     /translation="MPWNLPIFLTWLRVLLIPVMTALFYLPFPWFAEETINLTAAVIF
                     AVAALTDWFDGFLARLWKQTSDFGAFLDPVADKLMVAVSLLLLVKLDRTYVLFAMIII
                     GREITISALREWMAQMGKRNSVAVATVGKFKTAAQMLAIFLLLLNIPDFYGFNLVVIG
                     NVLMFIASLLTVWSMLYYLKMAWKEIA"
     gene            complement(3518..3941)
                     /locus_tag="WIC03_000075"
                     /pseudo
     CDS             complement(3518..3941)
                     /locus_tag="WIC03_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3981..4091)
                     /locus_tag="WIC03_000076"
                     /pseudo
     CDS             complement(3981..4091)
                     /locus_tag="WIC03_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(4224..4559)
                     /locus_tag="WIC03_000077"
     CDS             complement(4224..4559)
                     /locus_tag="WIC03_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEZ3882648.1"
                     /translation="MFKDWKEHTALVKKSFGELGKAHPKMLQAYGALEQAAAAEALDA
                     KTRELIAIAVAITTRCESCISVHAAAAAKAGATDSEIAGALATAIALNAGAAYTYALR
                     ALEAVETQK"
     gene            4688..5593
                     /gene="mtrA"
                     /locus_tag="WIC03_000078"
     CDS             4688..5593
                     /gene="mtrA"
                     /locus_tag="WIC03_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter MtrCDE transcriptional
                     activator MtrA"
                     /protein_id="HEZ3882649.1"
                     /translation="MDILDKLVDFAQLTGSVDVQCLLGGQWSVRHETLQREGLVHIVT
                     SGSGYLCIDGETSPRPVSTGDIVFFPRGLGHVLSHDGKCGESLQPDMRQHGAFTVKQC
                     GNGQDMSLFCARFRYDTHADLMNGLPETVFLNIAHPSLQYVVSMLQLESKKPLTGTVS
                     MVNALSSVLLVLILRAYLEQDKDVELSGVLKGWQDKRLGHLIQKVIDKPEDEWNVDKM
                     VAAANMSRAQLMRRFKSRVGLSPHAFVNHIRLQKGALLLKKNPDSVLSVALSVGFQSE
                     THFGKAFKRQYHVSPGQYRKEGGQK"
     gene            complement(5655..6143)
                     /locus_tag="WIC03_000079"
     CDS             complement(5655..6143)
                     /locus_tag="WIC03_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEZ3882650.1"
                     /translation="MKKYLIPLSIVAVLSGCQSIYVPTLTEIPVNPINTVKTEAPAKG
                     FRLASSHWTDVAKISDEATRLGYQVGIGKMTKVQAAQYLNNFRKRLVGRNAVDDSMYE
                     IYLRSAIDSQRGAINTEQSKLYIQNALRGWQQRWKNMDVKPNNPAFTNFLMEVMKMQP
                     LK"
     gene            6290..7192
                     /locus_tag="WIC03_000080"
     CDS             6290..7192
                     /locus_tag="WIC03_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ3882651.1"
                     /translation="MDTAKKDILGSGWMLVAAACFTIMNVLIKEASAKFALGSGELVF
                     WRMLFSTVALGAAAVLRRDTFRTPHWKNHLNRSMVGTGAMLLLFYAVTHLPLATGVTL
                     SYTSSIFLAVFSFLILKERISVYTQAVLLLGFAGVVLLLNPSFRSGQETAALAGLAGG
                     AMSGWAYLKVRELSLAGEPGWRVVFYLSVTGVAMSSVWATLTGWHTLSFPSAVYLSCI
                     GVSALIAQLSMTRAYKVGDKFTVASLSYMTVVFSALSAAFFLGEELFWQEILGMCIII
                     LSGILSSIRPTAFKQRLQSLFRQR"
     gene            complement(7233..8363)
                     /locus_tag="WIC03_000081"
     CDS             complement(7233..8363)
                     /locus_tag="WIC03_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ3882652.1"
                     /translation="MAKHLPLAVLTALLLAACGGSDKPPAEKPAPAENQNVLKIYNWS
                     EYVDPETVADFEKKNGIKVTYDVYDSDETLESKVLTGKSGYDIVAPSNAFVGRQIKAG
                     AYQKIDKSLIPNYKHLNPEMMRLMDGVDPGHEYAVPFYWGTNTFAINTERVKKALGTD
                     KLPDNQWDLVFDPEYTSKLKQCGISYLDSAAEIYPMVLNYLGKNPNSSNTEDIREATA
                     LLKKNRPNIKRFTSSGFIDDLARGDTCVTIGFGGDLNIARRRAEEAGGKEKIRVMMPK
                     EGVGIWVDSFVIPKDAKNVANAHKYINDFLDPEVSAKNGNFVTYAPSSKPARELMEDE
                     FKNDNTIFPTEEDLKNSFIMVPIQPAALKFMVRQWQDVKAGK"
     gene            8568..11192
                     /gene="alaS"
                     /locus_tag="WIC03_000082"
     CDS             8568..11192
                     /gene="alaS"
                     /locus_tag="WIC03_000082"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEZ3882653.1"
                     /translation="MKTSELRQKFLKFFETKGHTVVRSSSLVPHDDPTLLFTNAGMNQ
                     FKDVFLGFDKRPYSRATTAQKCVRAGGKHNDLENVGYTARHHTFFEMMGNFSFGDYFK
                     RDAIHFAWEFLTSPEWLNIPKDKLLATVYAEDDEAYNIWLNEIGMPSERIVRIGDNKG
                     AKYASDNFWQMGDTGPCGPCSEIFYDHGEEIWGGIPGSPEEDGDRWIEIWNCVFMQFN
                     RDEQGNMNPLPKPSVDTGMGLERMAAVMQHVHSNYEIDLFQDLLKAVARETGAAFSMD
                     EPSLKVIADHIRSCSFLIADGVLPSNEGRGYVLRRIIRRAVRHGYKLGQSKPFFHKLV
                     TDLVKEMGDAYPELKEKQAQIEEALKNEESRFAQTLETGMALLENALAKGGKTLDGEI
                     IFKLYDTYGFPYDLTADICRERNIELDEAGFEREMEAQRARARAAQSFKANAQLPYDG
                     QDTEFKGYSERQTESKVLALYKDGEQVNELNEGDSGAVVIDFTPFYAESGGQVGDVGY
                     IFSGENRFEVRDTQKIKAAVFGQFGVQTSGRLKVGDSVTAKVDDEIRNANMRNHSATH
                     LMHKALRDVLGEHVEQKGSLVTAESTRFDISHPQAVTAEEIAEVERRVNEAILANVAV
                     NAAIMSMEDAQKTGAMMLFGEKYGDEVRVLQMGGFSTELCGGTHVSRTGDIGLFKIIS
                     EGGIAAGVRRIEAITGLNALKWAQEQERLVKDIIAETKAQTEKDVLAKIQAGAAHAKA
                     LEKELARAKAELAVHAGAKLLDDAKDLGAAKLVAAQIEADAAALREIVTDLTGKSDNA
                     VILLAAVNDGKVSLCAGVSKALTGKVKAGDLVKFAAEQVGGKGGGRPDLAQAGGTDAD
                     KLPAVLDSVKDWVGAKLV"
     gene            complement(11281..12303)
                     /locus_tag="WIC03_000083"
     CDS             complement(11281..12303)
                     /locus_tag="WIC03_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEZ3882654.1"
                     /translation="MLTKKEFEKFLDEVCTVLRQEAKNAPFKSQDIFENRVRALCIAK
                     IPAYADIVINPEPVPQIFPDVPIGEYGVEVKFTLKDTWRSVANSIQEKNKADGVQHIY
                     VVFGKMGGIPDVKWQLYEDSVIHVRTSHVPRFEVEIESDRPSLFEGFGISYAEFADLD
                     MHEKMEYIRKYARNRLKEGERLWWIEDIDAVDSHDLPLEVRLYTSLPQEEKIRLRAEA
                     ALVSPRIVCSGRAKHKYDNAVLYLITYRGVLCHQARDLFSAGSVANSAEDYRGGNYVE
                     KSLKLIEHEIEKAALEMDDALIVEYWGESVPTEKRLEYWIKMLDDLADGWIPSESLFN
                     GRYKAK"
     gene            complement(12357..13508)
                     /locus_tag="WIC03_000084"
     CDS             complement(12357..13508)
                     /locus_tag="WIC03_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEZ3882655.1"
                     /translation="MTEFARKNWNKLAGDAIKAKMHEHGIGFIQLSELFLEQGESETA
                     NAICNKVNRGTFSHAFYLKCMELMTKQEKQEDREKKYKIISLFSGCGGMDLGFLGGFE
                     FLNRTYDKHDFDIIWANEIDEHAVRTYRHNLGNHIVHGDIWQHLDEIPTSADIIIGGF
                     PCQDISINGKGAGISGSRSGLYKAMLSAVERVRPKIFVAENVKGLLMKHHRKDLETVL
                     SDFRNLGYQVDYYLLNAADYGVPQTRERVIIVGRLPEIPEIQIEKTNTSNSYVTARQA
                     IGDLVDKVEDEIANHIWSRAEKSPDQGSRKLDAERAGYTIRAECHGNIQWHYELPRRI
                     SMREAARIQSFPDNFIFQAKLRATERQIGNAVPPVLGWHIAAAVSKALK"
     gene            14318..14548
                     /locus_tag="WIC03_000085"
     CDS             14318..14548
                     /locus_tag="WIC03_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3882656.1"
                     /translation="MFKTLKSIWQGLTQKGKIFPYQLAFTLLIPLRNWALSPQQITQR
                     LHFVPHHRICCGAGYFSCVGCASAHRFLIFAC"
     gene            14789..15733
                     /gene="tehA"
                     /locus_tag="WIC03_000086"
     CDS             14789..15733
                     /gene="tehA"
                     /locus_tag="WIC03_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HEZ3882657.1"
                     /translation="MSASKKFPIPLSYFSIALGLFALGLSWRYGASVGLLPALADESL
                     LAAASVVWLLLVAAYLIKMFAYRNDFLSDLRDLVQCCFISAIPITAMLEGLALKPYQA
                     GAAAVLIYAGVAGQLAFSMYRAAGLWRGLHSLEATTPIIYLPTVATNFVSASSLAALG
                     HHDYAALFFGAGMFSWLSLEASILGRLRTAAPVGTAARGVVGIQLAPAFVGCGAYFAV
                     GGKVDGFALALIGYGCLQLLFLLRLTRWFWEGGFTMSFWGFSFGFAAMAGCGLHLAAS
                     GVLSGLGLTLATAGSAGVALLLVGTLHRIATGRFLVRG"
     gene            complement(15807..16490)
                     /locus_tag="WIC03_000087"
     CDS             complement(15807..16490)
                     /locus_tag="WIC03_000087"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEZ3882658.1"
                     /translation="MELVFIRHGQSEWNAKNLFTGWRDVKLSGQGLAEAAAAGKKLKE
                     NGYEFDIAFTSVLTRAIKTCNIVLEESDQLFVPQIKTWRLNERHYGRLQGLDKKQTAE
                     QYGDEQVRIWRRSYDTLPPLLDKDDEFSAHKDRRYAHLPADVVPDGENLKVTLERVLP
                     FWEDQIAPAILSGKRVLVAAHGNSLRALAKHIEGISDKDIMGLEIPTGQPLVYKLDDN
                     LKVIEKFYL"
     gene            16786..19089
                     /gene="parC"
                     /locus_tag="WIC03_000088"
     CDS             16786..19089
                     /gene="parC"
                     /locus_tag="WIC03_000088"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEZ3882659.1"
                     /translation="MNMQPHAPHTDSNTLMLGRYAERAYLEYAMSVVKGRALPEVSDG
                     QKPVQRRILFAMRDMGLTAGAKPVKSARVVGEILGKYHPHGDSSAYEAMVRMAQDFTL
                     RYPLIDGIGNFGSRDGDGAAAMRYTEARLTPIAELLLSEINQGTVDFVPNYDGAFDEP
                     LHLPARLPMVLLNGASGIAVGMATEIPSHNLNEVTQAAIALLKKPTLETADLMQYIPA
                     PDFAGGGQIITPADELRRIYETGKGSVRVRARYEIEKLARGQWRVIVTELPPNANSAK
                     ILAEIEEQTNPKPKAGKKQLNQDQLNTKKLMLDLIDRVRDESDGEHPVRLVFEPKSSR
                     IDTDTFINTLMAQTSLEGNVSMNLVMMGLDNRPAQKNLKTILQEWLDFRTVTVTRRLK
                     FRLNQVEKRLHILEGRLKVFLHIDEVIKVIRESDDPKADLMAAFGLTEIQAEDILEIR
                     LRQLARLEGFKLEKELNELREEQGRLNILLGDENEKRKLIIKEMQADMKQYGDARRTL
                     VEEAGRAVLTQTTADEPITLILSEKGWIRSRAGHNLDLSQTAFKEGDRLKQTLEGRTV
                     LPVVILDSLGRTYTLDAAEIPGGRGDGVPVSSLIELQNGAKPVAMLTGLPEQHYLLSS
                     SGGYGFIAKLADMVGRVKAGKVVMTTDSGETVLPPIAVYASSFINPDCKIIAATSQNR
                     ALAFPIGELKIMAKGKGLQIIGLNAGESLTHTAVSSEPEILIESEGRRGAAHKDRIPV
                     SLLEAKRGKKGKLLPISGSLKQLSSPK"
     gene            19110..20642
                     /locus_tag="WIC03_000089"
                     /pseudo
     CDS             19110..20642
                     /locus_tag="WIC03_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            20694..21161
                     /locus_tag="WIC03_000090"
     CDS             20694..21161
                     /locus_tag="WIC03_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEZ3882660.1"
                     /translation="MTELQHPVLVVDDETDILDLMEMTLMKMGLRVHTASGVAEAKNK
                     LDSQRYSLVLTDMRMPDGSGLEVVQHINSRLLDTPVAVITAFGNADQAQEALRCGAFD
                     PDTMQIQDYLDQIERDIIEQTLKQTEGNRTQADKRLGISFRSMRYRMERLNIG"
     gene            21265..22017
                     /locus_tag="WIC03_000091"
     CDS             21265..22017
                     /locus_tag="WIC03_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ3882661.1"
                     /translation="MTDILIDNTATETVRTLIRAFPLVPVSQPPEQGSYLLTEHDTVS
                     LRLVGEKSSVIVDFASGAAQYRRTKGGGELIAKAVNHTAKPTVWDATAGLGRDSFVLA
                     SLGLTVTAFEQHPAVACLLSDGIRRALLNPETQDTAARINLHFGNAAEQMPALVQTQG
                     KPDIVYLDPMYPERRKSAAVKKEMAYFHRLVGEAQDEAVLLHTARQTAKKRVVVKRPR
                     LGEHLAGQAPAYQYTGKSTRFDVYLPYEADKG"
     gene            complement(22402..22608)
                     /locus_tag="WIC03_000092"
     CDS             complement(22402..22608)
                     /locus_tag="WIC03_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2788 domain-containing protein"
                     /protein_id="HEZ3882662.1"
                     /translation="MDEAVFADWALKICLTGLIIFLGFIVWNLGKESKAGKFGIAILF
                     LVLGLGVFGFVFKELLIKFLVLPK"
     gene            complement(22695..23864)
                     /gene="metZ"
                     /locus_tag="WIC03_000093"
     CDS             complement(22695..23864)
                     /gene="metZ"
                     /locus_tag="WIC03_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HEZ3882663.1"
                     /translation="MSKKLHPQTLAIRGGKEQTEYREHNQALFLTSSFMWDNAQHAAD
                     LFSKKIKGFTYTRTANPTTAAFEKRIAALEGAERAVATSTGMSAIQAAFFTFLQAGDH
                     VVSSRSLFGTTVGFINNIVTKFGIEVSHVSPTDINEWKAAVKANTKLLFLETPSNPLG
                     EVADLEALAELAHGIGAILVVDNSLLSPVGSQPLKHGADISVSSATKAIDGHGRVMGG
                     VLAGSEELMAQAAVYCNSCGLAMSPFNAWQLLSGVETLSLRMEKQFDNALKIAQWLQA
                     QPQVQAVYYTGLPDHPQAALIRKQQNGGGIVIGFEVADQAAAWKVVDGVELFSRTANL
                     GDVRSTITHPWTTTHGRMQPEEKLAANIRPGLVRLSVGLEYVGDLIDDLKQALAR"
     gene            complement(24009..24770)
                     /locus_tag="WIC03_000094"
     CDS             complement(24009..24770)
                     /locus_tag="WIC03_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3882664.1"
                     /translation="MDANKEPNWEKRLENLRQTRRMKAVPTYAPLSRPSETGTDAPVP
                     KLDDESRARVLKAYLKKWQEEHNAAVADCGEEVETDPMIVLQENWLNAQASLQMSVSE
                     KHIESRRRIRFDAKKDSAEFETGQIEGRENADAAESGTAADGVAENGEGGAEEALMPV
                     QINILNPKAVNRREVFCLSEQELTERLIKRLRPHLTDTVNGIIRTAVQKQMAVFTYQL
                     QQMLHEQAGAVVEDVLEHDVRKILNDIKYELKYKR"
     gene            24870..25121
                     /locus_tag="WIC03_000095"
     CDS             24870..25121
                     /locus_tag="WIC03_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3882665.1"
                     /translation="MGSIEQRLEYLEEANDVLRMQNHVLSTAFKALIRALPAETAEIA
                     VESIQLAFEDALAELSYEDSPHTDLFHDVTYAFFREKER"
     gene            25173..25979
                     /locus_tag="WIC03_000096"
     CDS             25173..25979
                     /locus_tag="WIC03_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEZ3882666.1"
                     /translation="MNMKKWIAAALACSALALSACGGQGKDAAAPAANPDKVYRVASN
                     AEFAPFESLDSKGNVEGFDVDLMNAMAKAGNFKIEFKHQPWDSLFPALNNGDADVVMS
                     GVTITDDRKQSMDFSDPYFEITQVVLVPKGKKISSSDDLKNMNKVGVVTGYTGDFSVS
                     KLLGNDNPKIARFENVPLIIKELENGGLDSVVSDSAVIANYVKNNPTKGMDFVTLPDF
                     TTEHYGIAVRKGDEATVKMLNDALEKVRESGEYDKIYAKYFAKEDEQAAK"
     gene            26286..27809
                     /locus_tag="WIC03_000097"
     CDS             26286..27809
                     /locus_tag="WIC03_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEZ3882667.1"
                     /translation="MTVIKQEDFIQSICDAFQFISYYHPKDYIDALYKAWQKEENPAA
                     KDAMTQILVNSRMCAENNRPICQDTGIATVFLKVGMDVQWDADMSVEEMVNEGVRRAY
                     TWEGNTLRASVLADPAGKRQNTKDNTPAVIHMSIVPGDKVEVTCAAKGGGSENKSKLA
                     MLNPSDNIVDWVLKTIPTMGAGWCPPGILGIGIGGTPEKAVLMAKESLMSHIDIQELQ
                     EKAASGAELSTTEALRLELFEKVNALGIGAQGLGGLTTVLDVKILDYPTHAASKPIAM
                     IPNCAATRHVEFELDGSGPVELTPPRVEDWPDLTYSPDNGKRVDVDKLTKEEVASWKT
                     GDVLLLNGKILTGRDAAHKRLVDMLNKGEELPVDFTNRLIYYVGPVDPVGDEVVGPAG
                     PTTATRMDKFTRQMLEQTGLLGMIGKSERGAAACEAIADNKAVYLMAVGGAAYLVAKA
                     IKSSKVLAFPELGMEAIYEFEVKDMPVTVAVDSKGESIHATAPPQWQAKIGIIPVKS"
     gene            27907..29319
                     /gene="trkA"
                     /locus_tag="WIC03_000098"
     CDS             27907..29319
                     /gene="trkA"
                     /locus_tag="WIC03_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEZ3882668.1"
                     /translation="MKILILGNGQVGSTVAQNLAAIPNNDVTVIDIDEKALQETGSRL
                     DVQTVFGNGASPFTLERAGAEDADLLLALSRSDETNIVACKVAADLFNIPGRIARVRS
                     SEYLEYLSPKLENNENGSLSIFGITETISPEQLVTEQLAGLIDCPGALQVLRFADDRV
                     RMVIIQARRGGLLVGRSIADIAQDLPDGADCQICAVYRNNRLIVPAPQTVIIEGDEIL
                     FAAAAENIGAVIPELRPKETSTRRIMIAGGGNIGYRLAKQLEHAYNVKIIECRPRRAE
                     WIAENLDNTLVLQGSATDETLLDNEYIDEIDVFCALTNDDESNIMSALLAKNLGAKRV
                     IGIVNRSSYVDLLEGNKIDIVVSPHLITIGSILAHIRRGDIVAVHPIRRGTAEAIEVV
                     AHGDKKTSAIIGRRISGIKWPEGCHIAAVVRAETGETIMGHHTETVIQDGDHIIFFVS
                     RRRILNELEKLIQVKMGFFG"
     gene            complement(29833..29946)
                     /locus_tag="WIC03_000099"
                     /pseudo
     CDS             complement(29833..29946)
                     /locus_tag="WIC03_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705522.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            30087..30162
                     /locus_tag="WIC03_000100"
     tRNA            30087..30162
                     /locus_tag="WIC03_000100"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30121..30123,aa:Glu,seq:ttc)
BASE COUNT         7429 a         8487 c         7433 g         7122 t
ORIGIN      
        1 ccccacgcta aataaaaaat agggaacaaa tatgtggaaa atgttgaaac acatagccca
       61 aacccaccgc aagcgattga ttggcacatt ttccctggtc ggactggaaa accttttgat
      121 gctggtgtat ccggtgtttg gcggccgggc gatcaatgcc gtgattgcgg ggggggtgtg
      181 gcaggcgttg ctgtacgctt tggttgtgct tttgatgtgg ctggtcggtg cggcgcggcg
      241 gattgccgat acgcgcacgt ttacgcggat ttataccgaa atcgccgtgc cggtcgtgtt
      301 ggaacagcgg cagcgacaag tcccgcattc ggcggtaact gcgcgggttg ccctgtcgcg
      361 tgagtttgtc agcttttttg aagaacacct gccgattgcc gcgacatccg tcgtatccat
      421 attcggcgcg tgcatcatgc tgctggtgct ggaattttgg gtcggcgtgt cggcggtggg
      481 catacttgcg ttgtttttat ggcttttgcc acgttttgcc gccatcagcg aaaacctgta
      541 tttccgcctg aagaacagct tggaatgcga caaccacttt atccgaaaag gagaccggcg
      601 gcagctggac cgccattacg gactggttgc gcgcctgcgt gtgctgattt ccaaccgcga
      661 agccttcggc tatctctgcg tcggcgcggc gatgggtatt ttgttcggct ttgcttttgt
      721 gatgatgacg ctcaaaggct acagcagcgc ggggcatgtc tattcggtcg gcacttatct
      781 gtggatgttt gccatgagtt tggacgacgt gccgcgattg gtcgaacaat attccaattt
      841 gaaagacatc ggacaacgga tagagtggtc ggaacggaac atcaaagccg gaacttgaaa
      901 aatgccgtct gaacacgctt cagacggcat ttccatccgt tcggcaaact acatcacatc
      961 cgcccgccgg ttgacaagtt tggcaaacaa cttttcaaca gaagcttccg cctgcaaacc
     1021 aatgcgctgg atcaggcttt gcttctcctg atatttcact tcgataacct gtttgttttc
     1081 aaacgctttc aacaacaaat catcactggt cgaaatctcg tcaatcaagt tcaacgccaa
     1141 cgcctgccga ccgaaccaat gctcgcccgt tgccacttcc tcaatatcca attgagggcg
     1201 gttctcgctg acaaactgct tgaacaactg atgcgtttcc tccagttcct gtcggaattt
     1261 ctgtttgccc ttttccgtat tttcacccat aaaagtaacc gtgcgcttaa attcgcccgc
     1321 cgtcatcaca tccacatcaa tatcatgttt tttcaacagg cggtggatat tcggtacttc
     1381 cgccaccaca cccaccgaac cgacaatcgc aaacggagcg gaaacaattt tatccgccac
     1441 acacgccatc atataaccac cgctcgctgc caccttatcg acggcgacgg tcagcggaat
     1501 attgcgttcg cgcaaacgcc taagctgtga agccgccaaa ccgtaaccgt gaaccacgcc
     1561 gcccggactt tccaatctaa gcagaacctc atcttcaggc ttggcaatca aaagcaccgc
     1621 cgtaatctca tgacgcaagg attctacggc gtgtgcatac aaatcgccgt caaaatccaa
     1681 cacaaaaagg cgggattttt gcgtttcgga agatttctcc ccaccctcct tcaaacgctt
     1741 tttctctgct ttggcttccg ccttttcctt tttcttttcc tctttttcct gatgttttgc
     1801 ctcttccccg cttaaaaaga atgcttcaaa cgattgccgc tgttttttat aattttccga
     1861 aaaatccgtc agtacgacac tgccgctttc cgactgtttc ttactctgta cgatagccaa
     1921 cacaatcagc gcaattgcgc cgaacacggt aagcagttcg agcaggaaaa taccgtaatt
     1981 cagtaaaatt tctttccaca ttgattggat ttcctcttgt tcaggcatga acatgtcaat
     2041 attgtccatc accgtccgac agataaaaaa ataaccgctt ggagcggcat tgtcattttc
     2101 agcttggtgc ccggagccgg aatcgaaccg gcacgggatg tttagtcccg acggatttta
     2161 agtccgttgt gtctacctat ttcaccaccc gggcatttgt gaaaggtgga ggcgggggcg
     2221 cggattttaa ccggcctata tgaagattgc actcctcata gcataaacac tctgccaccc
     2281 cgccatagta cgataatgga ggcgagagtc ggaatcgaac cggcgtagac ggatttgcaa
     2341 tccgctgcat aaccactttg ctatctcgcc ctaaaactgg cttatctaaa aaaacttgga
     2401 gcgggaaacg agtctcgaac tcgcgacctc aaccttggca aggttgcgct ctaccaactg
     2461 agctattccc gcgcgttcaa acatatcggt ttttggagcg gaaaacgagt ctcgaactcg
     2521 cgacctcaac cttggcaagg ttgcgctcta ccaactgagc tattcccgcg ttgatatgtt
     2581 tgaaataaaa cttggagcgg aaaacgagtc tcgaactcgc gacctcaacc ttggcaaggt
     2641 tgcgctctac caactgagct attcccgcaa tgattgtggg aaaatgaaat ttttggagcg
     2701 ggaaacgagt ctcgaactcg cgacctcaac cttggcaagg ttgcgctcta ccaactgagc
     2761 tattcccgcc cgatttcatt ctccaacatc gaagagacgc aattattatg gattctgttt
     2821 ttgccgtcaa gctattttta tgtttttttc aggcgatttc tttccacgcc attttcagat
     2881 aatacagcat cgaccagact gtcagcaaag atgcgataaa catcaataca ttgccgataa
     2941 caacaagatt aaatccgtaa aaatcgggaa tattcagcag cagcaggaaa attgccagca
     3001 tttgcgcggc ggttttaaac ttaccgacgg tggcgacggc aacgctgttc cttttgccca
     3061 tttgcgccat ccattcgcgc aatgcggaaa tggtaatttc cctgccgata atgatcatgg
     3121 caaacaaaac ataggtccgg tcgagtttga ccagtaaaag tagcgatacg gcaaccatca
     3181 gcttgtcggc gacgggatcg aggaaggcgc cgaaatcgga agtctgtttc cacagtcttg
     3241 ccaaaaatcc gtcaaaccag tcggttaagg cggcaacggc aaaaatgacg gcggcggtga
     3301 gattaatcgt ttcctccgcg aaccacggaa aaggcaggta aaaaagggct gtcatgacag
     3361 gaatgagcaa gaccctcaac catgtgagga agatggggag attccaaggc atcggttttc
     3421 tctgtgcaga ctgtaaagtt gtgattataa cggttatcct cataacccaa aacgtaaaat
     3481 tgctgcatgg gcattccccc gccccgccaa tctgttttca cattcttttc aaacgcagga
     3541 aaatggcggg caataaaagc aaaataccca gtttcaggct gaaaacggca ggttgtgcca
     3601 acacttcgac aaggcggtct tccgtgcggg caaaatcttt attgcttata gacactgcca
     3661 ctgttgcggt attccaacag aacgccgttt aaaaaacctt tgccgacggt ttcgcttaaa
     3721 acggctctaa cctgctccgc cctgatggtt ctgccgatat tgccttccgc gcacaaaccg
     3781 tcgaacccat agcaggaaag ccggtaatgc tgcccgtctg catccagttt gattgcccgt
     3841 ccgctgcggt tgggggcggt aacggtcaat tccgcatatt cgaatgtttt ttcttgttcg
     3901 tgaaatgccg tcaggtaagg tgcaataaaa acggcggaca acagcagaca gcttatggcg
     3961 gcaaaccata cccagcgata atatagtgga ttaaatttaa accagtacag cgttgcctcg
     4021 ccttgccgta ctatttgtac tgtctgcggc ttcgtcgcct tgtcctgatt taaatttaat
     4081 ccactatatt tcacgcttac cccttgtttc tcaaatgccg tctgaaaaaa cggctgcata
     4141 tgttgtctac aaaatatcgg gaagcataac agacaaaatg ccgtctgaaa tattttcaga
     4201 cggcatttct tatccgaaac ggattatttt tgcgtttcaa ccgcttccaa tgcacgcagg
     4261 gcgtaagtgt aagcggcacc ggcattcagg gcgatggcgg ttgccaatgc gcctgcgatt
     4321 tcgctgtcgg tcgcaccggc tttggcggca gcggcggcgt gaacgctgat gcagctctcg
     4381 caacgagtgg taatggcaac ggcgatggca atcagttcgc gcgttttggc atcaagtgct
     4441 tcggcggcgg cggcctgttc caatgcgccg taggcctgca gcattttagg atgcgcctta
     4501 cccagctcgc cgaacgattt tttaaccaat gcggtatgtt ctttccaatc tttaaacatt
     4561 ttcttttcct ttctcttgcg tttaaccctg atacgcgctt gcgtatctgt tttcgatgtg
     4621 cgtattattg caattattca gttgtgtttc tcgtttaatc atctcatttt atggttcaaa
     4681 aagatttatg gacattctgg acaaactggt cgatttcgcc caattgacgg gcagtgtgga
     4741 tgtgcagtgc cttttgggcg gacaatggtc ggtacggcat gaaaccttgc aacgcgaagg
     4801 actggtacac attgttacat cgggcagcgg ctatctctgc atcgacggcg aaacttcccc
     4861 gcgtccggtc agtacagggg atattgtatt tttcccgcgc ggcttgggtc atgtgttgag
     4921 ccacgacgga aaatgcggag aaagtttaca accggatatg cggcagcacg gtgcgtttac
     4981 ggtcaaacag tgcggcaacg gacaggatat gagcctgttt tgcgcccgtt tccgctacga
     5041 cacccacgcc gatttgatga acgggctgcc tgaaaccgtt tttctgaaca ttgcccatcc
     5101 gagtttacag tatgtggttt caatgctgca actggaaagc aaaaaacctt tgacggggac
     5161 ggtttccatg gtcaacgcat tgtcgtccgt cctgctggtg cttatcctgc gcgcctatct
     5221 cgaacaggat aaggatgtcg aactctcggg cgtattgaaa ggttggcagg acaaacgttt
     5281 gggacattta atccaaaagg tgatagacaa accggaagac gaatggaatg tcgacaaaat
     5341 ggtggcggct gccaatatgt cgcgcgcgca actgatgcgc cgtttcaaaa gccgggtcgg
     5401 actcagcccg cacgcctttg tgaaccatat ccgcctgcaa aaaggcgcgt tgctgctgaa
     5461 aaaaaacccg gattcggttt tgtcggtcgc actgtcggta ggctttcagt cggaaacgca
     5521 cttcggcaag gcgttcaaac ggcaatatca cgtttcgccg ggtcaatacc ggaaagaagg
     5581 cgggcaaaaa taaatcgggg cttcaaacgc aaatgccgtc tgaaaaggct ttcatacagc
     5641 atttgcgtac cgcgtcattt caagggctgc atcttcatca cttccatcaa aaagttggta
     5701 aatgcggggt tgttgggttt gacatccata tttttccaac gctgctgcca gccgcgcaag
     5761 gcattctgga tatacagctt ggactgttcc gtattgattg cgccccgctg gctgtctatc
     5821 gccgaacgca ggtagatttc atacatactg tcatcgacgg cattgcgtcc gaccaggcgt
     5881 tttctgaagt tgttcagata ttgcgccgcc tgaaccttgg tcattttacc gatacccacc
     5941 tgatagccca agcgcgtcgc ttcatcgctg attttggcaa catccgtcca atgcgaagag
     6001 gcaaggcgga aaccttttgc aggtgcttcc gttttgacgg tattgatagg attcacgggg
     6061 atttccgtca atgtgggcac ataaatagac tggcagccgg aaagaactgc cacaatggaa
     6121 agagggataa ggtatttttt catgccccca ttataatcaa gtttgccttg agaaaacaaa
     6181 ttgttcggca agaaaaataa aatttcggca tcagaagcag gcaaaaacac attccacaag
     6241 ccttgccgca aggtttacaa tccgaccgtc cttatcgcaa cgaccgttta tggataccgc
     6301 aaaaaaagac attttaggat cgggctggat gctggtggcg gcggcctgct ttaccattat
     6361 gaacgtattg attaaagagg catcggcaaa atttgccctc ggcagcggcg aattggtctt
     6421 ttggcgcatg ctgttttcaa ccgttgcgct cggggctgcc gccgtattgc gtcgggacac
     6481 cttccgcacg ccccattgga aaaaccactt aaaccgcagt atggtcggga cgggggcgat
     6541 gctgctgctg ttttacgcgg taacgcatct gcctttggcc accggcgtta ccctgagtta
     6601 cacctcgtcg atttttttgg cggtattttc cttcctgatt ttgaaagaac ggatttccgt
     6661 ttacacgcag gcggtgctgc tccttggttt tgccggcgtg gtattgctgc ttaatccctc
     6721 gttccgcagc ggtcaggaaa cggcggcact cgccgggctg gcgggcggcg cgatgtccgg
     6781 ctgggcgtat ttgaaagtgc gcgaactgtc tttggcgggc gaacccggct ggcgcgtcgt
     6841 gttttacctt tccgtgacag gtgtggcgat gtcgtcggtt tgggcgacgc tgaccggctg
     6901 gcacaccctg tcctttccat cggcagttta tctgtcgtgc atcggcgtgt ccgcgctgat
     6961 tgcccaactg tcgatgacgc gcgcctacaa agtcggcgac aaattcacgg ttgcctcgct
     7021 ttcctatatg accgtcgttt tttccgccct gtctgccgca ttttttctgg gcgaagagct
     7081 tttctggcag gaaatactcg gtatgtgcat catcatcctc agcggtattt tgagcagcat
     7141 ccgccccact gccttcaaac agcggctgca atccctgttc cgccaaagat aaaaaatgcc
     7201 gtccgaacat ccttcagacg gcatatcggg ctttatttcc ccgccttcac atcctgccac
     7261 tggcgcacca taaacttcaa tgccgccggc tggataggca ccatgataaa gctgtttttc
     7321 aaatcctcct cggttgggaa aatcgtattg tcgtttttaa attcgtcttc catcagctca
     7381 cgcgccggct tgctcgaagg cgcataagta acgaaattgc cgtttttcgc cgacacttcc
     7441 ggatcgagga agtcgttgat gtatttgtgc gcgttggcga cgtttttcgc atctttcgga
     7501 atcacgaaag aatccaccca aatccccacg ccctctttgg gcatcatcac gcggattttt
     7561 tccttgccgc ccgcttcttc ggcacgccgt ctggcgatgt tcaaatcgcc gccgaaaccg
     7621 attgttacgc aggtatcgcc gcgcgccaaa tcatcgataa agccggacga agtaaagcgt
     7681 ttgatattgg ggcggttttt cttgagcagg gcggttgcct ccctgatgtc ttccgtattg
     7741 ctgctgttcg ggtttttacc caaatagttc aacaccatag gataaatttc cgccgcgctg
     7801 tctaaataac tgataccgca ttgtttgagt ttggacgtgt attcggggtc gaacaccaaa
     7861 tcccactggt tgtcgggcag cttgtccgta cccaaagcct ttttcacgcg ttcggtattg
     7921 atggcgaagg tatttgtccc ccaataaaac ggcacggcgt attcgtggcc gggatcgacc
     7981 ccgtccatca gcctcatcat ttcagggttg agatgtttat aattgggaat cagtgactta
     8041 tcgattttct gataagcccc cgccttaatc tgcctgccca caaacgcatt ggacggcgcg
     8101 acaatgtcgt aaccggattt gccggtcagc accttgcttt ccagtgtttc gtcgctgtcg
     8161 tacacatcat aagtaacctt gatgccgttt ttcttttcaa aatcggcaac ggtttccgga
     8221 tcgacgtatt ccgaccagtt gtaaattttc aatacgtttt ggttttccgc cggtgccggt
     8281 ttttcggcag gcggtttgtc cgaaccgccg cacgctgcaa gcagcaaagc agtcaggacg
     8341 gcgagtggca gatgtttggc cattatcatt ccttgcatat cgggttggag aaagcggtca
     8401 ttatagccga tattggcaac agggcttcag acggcattca aaatcccgcc acacccttcc
     8461 gaaaaccgcc gcttccatag ctagaaacag ggatttgcgg taagataccg ccgttcgttt
     8521 tccctgcttt taccatgaca agacatttga gagacattga aaaaattatg aaaacctccg
     8581 aactgcgcca aaaattccta aaattttttg aaaccaaagg ccacaccgtc gtccgctcct
     8641 ccagcctcgt gccgcacgac gacccgaccc tgctgtttac caacgcgggc atgaaccaat
     8701 ttaaagacgt atttttaggt ttcgacaaac gcccgtacag ccgcgccacc accgcgcaaa
     8761 aatgcgtacg cgcaggcggc aaacacaacg acttggaaaa cgtcggctac accgcccgcc
     8821 accacacctt ctttgaaatg atgggcaact tctccttcgg cgactacttc aaacgcgacg
     8881 ccatccactt cgcttgggaa tttctgactt cccccgaatg gctcaacatc cctaaagaca
     8941 aactgttggc gaccgtttac gcggaagacg acgaagccta caacatctgg ttgaacgaaa
     9001 tcggtatgcc gtccgagcgc atcgtccgca tcggcgacaa caaaggcgcg aaatacgcat
     9061 ccgacaactt ctggcaaatg ggcgacaccg gcccttgcgg cccctgctcc gaaattttct
     9121 acgaccacgg cgaagaaatc tggggcggca ttcccggcag ccccgaagaa gacggcgacc
     9181 gctggatcga aatttggaac tgcgtattta tgcagttcaa ccgcgacgaa caaggcaata
     9241 tgaatccgct gcccaagccg tccgtcgata ccggcatggg tttggagcgc atggcggcag
     9301 taatgcaaca cgtccacagc aactacgaaa tcgacttgtt ccaagacctg ctcaaagccg
     9361 ttgcccgcga aaccggcgca gctttcagca tggatgaacc cagcctgaaa gtcatcgccg
     9421 accacatccg ctcctgctcc ttcctgattg cagacggcgt attgccgtcc aacgaaggcc
     9481 gcggctacgt attgcgccgc attatccgcc gcgccgtgcg ccacggttac aaactgggtc
     9541 aaagcaaacc gttcttccac aaactcgtta ccgatttggt caaagagatg ggcgatgcct
     9601 accctgaatt gaaagaaaaa caggcgcaaa tcgaagaagc gttgaaaaac gaagaaagcc
     9661 gttttgccca aactttggaa accggtatgg ctttgttgga aaatgcactg gccaaaggcg
     9721 gcaaaacact cgacggcgaa atcatcttca aactctacga tacttacggt ttcccatacg
     9781 acctgaccgc cgacatctgc cgtgaacgca atattgaact ggacgaagca ggcttcgagc
     9841 gcgaaatgga agcccaacgc gcacgcgcac gcgccgccca aagcttcaaa gccaacgccc
     9901 aactgcctta tgacggtcaa gacaccgagt ttaaaggtta tagcgaacgc caaaccgaat
     9961 ccaaagtcct cgccctctac aaagacggcg agcaagtcaa cgaattgaac gaaggcgaca
    10021 gcggcgcagt cgttatcgac tttaccccgt tctatgcaga atccggcggc caagtcggcg
    10081 acgtcggcta tatcttctca ggcgaaaacc gctttgaagt acgcgatacc caaaaaatca
    10141 aagcggccgt attcggtcaa ttcggcgtac aaacttcagg ccgtctgaaa gtcggcgaca
    10201 gcgttaccgc caaagtggat gacgaaatcc gcaatgccaa tatgcgcaac cacagcgcaa
    10261 cccacttgat gcacaaagcc ctgcgcgatg tattgggcga acacgttgaa caaaaaggct
    10321 cgttggttac cgccgaatcc acccgtttcg acatttccca tcctcaagcg gtaactgccg
    10381 aagaaatcgc cgaagtagaa cgccgcgtca acgaagccat tttggcgaac gttgccgtca
    10441 acgccgccat tatgagcatg gaagacgccc aaaaaaccgg cgcgatgatg cttttcggcg
    10501 aaaaatacgg cgacgaagta cgcgtactgc aaatgggcgg tttctctacc gaattgtgcg
    10561 gcggcacaca cgtttcacgc accggcgaca tcggtctctt caaaatcatc agcgaaggcg
    10621 gtattgccgc aggcgtgcgc cgtatcgaag ccatcaccgg cctgaacgcg ctcaaatggg
    10681 cgcaagagca agaacgcttg gttaaagaca ttattgccga aaccaaagcc caaaccgaaa
    10741 aagacgtact ggcgaaaatc caagcaggcg cggcacacgc caaagcattg gaaaaagaat
    10801 tggcacgcgc caaagccgaa ctcgccgtcc acgcaggcgc caaactcttg gacgatgcaa
    10861 aagacttggg cgcagccaaa ctcgttgctg cccaaatcga agccgacgca gccgccctac
    10921 gcgaaatcgt aaccgacctg accggaaaat ccgacaacgc cgtgattctt ttggcggcag
    10981 taaacgacgg caaagtctcc ctgtgcgcag gcgtatccaa agccctgacc ggaaaagtga
    11041 aagcaggcga tctggttaaa tttgcagccg aacaagtcgg cggcaaaggc ggcggcagac
    11101 cagatttggc gcaagccggc ggcacggatg ccgacaaatt gcccgccgtg ttggatagcg
    11161 tgaaagactg ggtcggcgcg aagctggttt gatgtgggaa aggcagcctg aaagggattc
    11221 aggctgcctt ttgtgttaaa gaggccgtct gaaagatatt ttcaaacggc ctctgtcagt
    11281 ttatttggct ttatacctac cattgaatag cgattcagaa ggtatccagc catctgccaa
    11341 atcatccaac atcttaatcc aatactctag gcgtttttct gtcgggacgc tttctcccca
    11401 atattcgaca attagagcat catccatttc caaagcagct ttttcaattt catgctcaat
    11461 caattttaaa gatttttcta catagtttcc gccacggtag tcttccgccg agttggcaac
    11521 gctgcccgca ctgaataagt cccgcgcctg atgacaaagc acaccgcgat aggtaatcag
    11581 atacaggacg gcattatcgt atttgtgctt cgccctgcct gagcagacaa tacgggggga
    11641 gactaatgct gcttcagctc ggaggcggat tttttcttcc tgcggcaggg aagtgtaaag
    11701 gcgcacttcc aaaggcagat catggctgtc tactgcgtca atgtcttcta tccaccataa
    11761 ccgctcgcct tctttcaacc tgttacgggc atattttcgg atatattcca ttttttcatg
    11821 catatccaaa tcggcaaatt cagcatagga aataccgaaa ccctcaaaca gagacggtcg
    11881 atcagattct atttctacct caaagcgcgg aacatgcgat gtgcggacat gaattacgct
    11941 atcttcgtaa agttgccatt tcacatcggg aatgcccccc atttttccga ataccacata
    12001 gatatgctgt acaccgtcag ctttgttttt ctcttggata ctgtttgcta cgctgcgcca
    12061 agtatctttt agcgtgaatt tgacttctac accatattcc cctattggaa catcggggaa
    12121 aatttgcggc acaggttcag gattgataac tatgtcagca taagcgggaa tttttgcaat
    12181 gcacagcgca cgaactctgt tttcaaaaat atcttgggat ttgaaaggtg cgttttttgc
    12241 ctcttgcctc aatacggtgc aaacctcgtc taaaaacttc tcaaactcct tttttgtcag
    12301 cataatgcat taccacttaa aaatataaag tagtaatttt atcttattta acagggttat
    12361 ttaagtgcct tactaacggc ggcggcgatg tgccacccta aaacaggagg gacggcgttg
    12421 ccaatttgcc gttctgtggc acgcaatttt gcctggaaaa taaaattgtc gggaaacgat
    12481 tggatgcgcg ccgcctcacg catggagatg cggcggggta attcgtaatg ccactgtatg
    12541 tttccatggc attctgccct aatagtgtag cccgcccttt cggcatccaa ttttcgacta
    12601 ccttgatcgg ggcttttttc tgctctactc caaatatggt tggcaatttc gtcttctacc
    12661 ttgtcgacca aatcgcctat agcctgtcta gctgttacat agctgttgct ggtatttgtt
    12721 ttttcaatct gtatttcggg aatttcaggt agccttccaa cgatgataac tcgctcccga
    12781 gtctgtggaa caccgtaatc ggctgcattc agcaagtagt aatctacctg ataacccaaa
    12841 ttgcgaaaat ctgaaagcac tgtttccaaa tctttccgat gatgcttcat cagtagccct
    12901 ttgacatttt cagcaacaaa tattttgggg cgaacgcgtt caacagcaga caacatggct
    12961 ttatacagcc cgctgcggct gcctgaaata cctgcacctt taccattaat agaaatatcc
    13021 tgacaaggaa agccaccgat aatgatgtct gcgctagtgg gtatttcatc caaatgctgc
    13081 caaatatcac catgtacaat gtgatttcct aagttgtggc ggtaggttct aacagcgtgt
    13141 tcgtcgattt cgttcgccca aataatatca aaatcatgtt tgtcataagt acggtttaaa
    13201 aactcaaagc cccccagaaa acccaaatcc attccgccac aaccgctaaa taaagaaata
    13261 attttatatt ttttttcgcg gtcttcttgc ttttcctgtt tggtcattaa ctccatacat
    13321 ttaagataaa aagcatggga aaaagtacct ctatttactt tgttacaaat tgcatttgcc
    13381 gtttcacttt ccccttgctc caagaataat tctgataatt gtataaagcc aattccatgc
    13441 tcatgcattt tggcttttat ggcatctcca gctaacttgt tccaattttt tcgtgcaaat
    13501 tcggtcatgg ttaatctttc agtcattcat aaatgacatt attttagcgt tttttataat
    13561 ttaagccaag atttaaaatc tacctttagg tttacaggcc gtctgaaaac aaaaatctcg
    13621 ttttcagacg acctttttcc gctaaaatcc ccctaccgtc caacccgaaa caaggagaac
    13681 catcatgcaa gttcctactt ccgccatcat caacggcgaa tttgccgaca aatacggcaa
    13741 acgcggcagc aagccgtagc cagcacccct gtcaaccgcg tgcgtggcta gaccacacac
    13801 cctaccactg cgggcgacgc aaaccttaag attgaggtgt agggtgtgtg cggtacgcac
    13861 gcacgcggtt tttgttacta ccgaatgatt ccaaaccatg ccgtaatcca gtcagcagca
    13921 aacgcagctt gggtcatgta ccaacaaaac tcaatatcaa aggccgtctg aagaataatt
    13981 ttcagacggc ttctctctat caaaagcagc ctgcacattc aaaaatcgaa gtgcaggctg
    14041 ctttttgtga tgattggaaa aaacacacac tatttgagac ctttgcaaaa atagtctgtt
    14101 aacgaaattt gacgcataaa aatgcgccaa aaaaattttc aattgcctaa aaccttccta
    14161 atattgagca aaaagtagga aaaatcagaa aagttttgta ttttgaaaat gagattgagc
    14221 ataaaatttt agtaacctat gttattgcaa aggtctctat ttttatactg tatattgtgc
    14281 aggtacaaag taaaacgaat tacccaaaga aattcctatg tttaagacgc tcaaaagcat
    14341 ttggcaggga cttacgcaaa aaggcaaaat cttcccgtac caactggcat tcacgctgct
    14401 gattcctttg cgtaactggg cgctttcacc gcaacaaatc acgcaacggc tgcactttgt
    14461 tccgcaccac cgaatttgct gcggggcggg ttatttcagt tgcgtaggat gtgcttcagc
    14521 ccaccgtttc ctcatttttg cttgttaatt ggtgggctga agcccacact acggttaata
    14581 cggttactga ttcgtttccc cctgcatccg aatcacgccg cacaccgcaa aaaacgcgcg
    14641 cccgatgcgc aaccgcttcc gacatctggc gcaatgagac aaaacggcaa tatgccgata
    14701 cggatacgtc atcctccgcc tgcctgtttt ttatatcata ctcaatttta tctgtgcgtt
    14761 acgacggcat gccgtctgaa gggtgtttat gtctgcatct aagaaatttc cgattccttt
    14821 gagctatttc agcatcgcgc tgggcttgtt tgccttgggg ctgtcgtggc gttacggcgc
    14881 gtctgtcggg ctgctgcccg ccttggccga cgaatcgctg cttgcggcgg cttcggtcgt
    14941 ctggctcttg ctggtggcgg catacctgat caaaatgttt gcgtaccgaa acgatttttt
    15001 gtctgatttg cgcgacttgg tgcaatgctg cttcatcagc gcgattccga ttaccgctat
    15061 gctggaggga ctcgcgctga agccctatca ggcaggcgcg gcggcagtcc tgatttatgc
    15121 cggcgttgcc ggacagttgg cgttttcgat gtatcgggcg gccggtctgt ggcgcggcct
    15181 gcattccttg gaggcgacga cgccgattat ttatctgcct acggttgcga caaactttgt
    15241 cagcgcgtca tctctggccg cgttggggca tcatgattat gcagctttgt ttttcggcgc
    15301 gggtatgttt tcctggctga gcttggaagc ctccatcttg ggcaggctgc gcacggcggc
    15361 accggtcggc acggcggcgc gcggcgtggt cggcatccag cttgcgcccg cctttgtcgg
    15421 ctgcggcgcg tattttgccg tcggcggtaa agtcgacggt tttgcgttgg cattaatcgg
    15481 ctacggctgc ctgcagcttt tgttcttgct gcgcctgacc cgctggtttt gggaaggcgg
    15541 ttttacgatg agcttttggg gattttcatt cggtttcgcg gcaatggcag gatgcggtct
    15601 gcatctggcg gcttctggcg tattgtcggg cttggggctg acgcttgcca ccgccggatc
    15661 ggcaggcgtg gcgctgctgc ttgtcggtac gctgcaccgg atagcgacgg ggcgtttctt
    15721 ggtacgcggc tgatgcgttt tgccgccttg tcaaaaatgc cgtctgaacg ctcaagcctt
    15781 cagacggcat tttttatttc acacccttac aggtagaatt tttcgatgac tttcaaattg
    15841 tcgtccaatt tgtacaccaa cggctgaccg gtcgggattt ccaagcccat gatgtctttg
    15901 tcggaaatgc cctcgatgtg ttttgccagc gcgcgcaggg agttgccgtg cgccgccacc
    15961 aagacgcgtt tgccgctcaa aatcgcgggg gcgatttggt cttcccaaaa cggcaatacg
    16021 cgctccagcg ttactttcag gttttcgccg tcgggtacga catcggcagg cagatgggca
    16081 tagcggcggt ctttgtgtgc ggaaaactca tcgtctttgt ccaaaagcgg cggcagggtg
    16141 tcgtagctgc gccgccagat gcggacttgc tcgtcgccgt attgttcggc ggtttgtttt
    16201 ttgtccaaac cttgcagtcg gccgtagtgg cgttcgttca gccgccatgt tttgatttgc
    16261 ggtacgaaca gttggtcgga ttcttccaaa acgatgttgc aggtcttaat cgcgcgggtc
    16321 aggacggatg tgaaggcgat gtcgaactca tagccgtttt ctttcagttt tttaccggcg
    16381 gcggcggcct cggcaagccc ctgcccgctc agcttcacgt cgcgccagcc tgtaaacagg
    16441 tttttcgcgt tccattcgct ttgtccgtgg cggataaata ccaattccat atcgtctcca
    16501 atgtgtgaaa gtgggaaagc cttatttata acatattttc acattcccgt atttgattca
    16561 gattcagaca cgcgcccact atggtttgcc gttttgattt acaataatgc cctttgcttt
    16621 acattccgca tacacaatgt ttcaacacac aggacgacac ataaagcacc gccctatgtg
    16681 ttgccctgat ttggaagggg ttgccccctc ccaaataaag tctgattcta ccgccccgaa
    16741 ggacagatgt ccaagtggcg ggggttcaac cgaaaaggaa atacgataaa tatgcaaccg
    16801 cacgctccac ataccgattc caatacgctg atgctcggcc gatacgccga acgcgcctat
    16861 ctcgaatacg ccatgagcgt ggtcaaaggc cgcgcgctgc ctgaagtttc agacggccaa
    16921 aagcccgttc agcggcgcat tttgtttgcg atgcgcgata tgggtttgac ggcgggagcg
    16981 aagccggtga aatcggcgcg cgtggtcggc gagattttgg gtaaatacca cccgcacggc
    17041 gacagttccg cctatgaggc gatggtgcgg atggcgcagg attttacctt gcgctatccc
    17101 ttaatcgacg gcatcggcaa cttcggttcg cgtgacggcg acggggcggc ggcgatgcgt
    17161 tacaccgaag cgcggctgac accgattgcg gaattgctgt tgtccgaaat caatcagggg
    17221 acggtggatt tcgtgccgaa ctacgacggc gcgtttgacg aaccgctgca cctgcccgcc
    17281 cgcctgccta tggtcttgct caacggtgcg tcaggcattg cggtgggtat ggcgaccgaa
    17341 atcccatcac acaatttgaa tgaagtcaca caggcggcga ttgcactgtt gaagaagccg
    17401 acgctggaaa ccgccgacct gatgcaatac atccctgctc ccgattttgc cggaggcggt
    17461 caaatcatca cgccggcgga cgaattgcgc cgtatttacg aaaccggcaa gggcagcgtg
    17521 cgcgtgcgtg cgcgttacga aatcgagaag ctggcgcgcg gacaatggcg cgtcatcgta
    17581 accgagctgc cgccgaacgc caattccgcc aaaatccttg ctgaaatcga agaacagacc
    17641 aacccgaaac cgaaagcagg caaaaagcag ctcaaccaag accagctcaa tactaaaaag
    17701 ctgatgctgg atttaatcga ccgcgtgcgc gacgagtcgg acggcgaaca tcccgtgcgc
    17761 cttgtatttg aaccaaaatc cagccgcatc gataccgata ccttcatcaa cacgctgatg
    17821 gcgcaaactt cgctggaagg caatgtgtcc atgaacttgg tgatgatggg gctggacaac
    17881 cgccccgcgc agaaaaacct gaaaacgatt ttgcaggaat ggttggattt ccgcaccgtt
    17941 accgtaacac gccgtctgaa attccgtttg aaccaagtgg aaaaacggct gcacatcctc
    18001 gaaggccgtc tgaaagtctt tctgcacatc gacgaagtga ttaaagtcat ccgcgaatca
    18061 gatgacccga aagccgattt gatggcggcg ttcgggctga ccgaaatcca agccgaagac
    18121 attttggaaa tccgcctgcg ccagttggca cgtttggaag gtttcaaact cgaaaaagaa
    18181 ttgaacgaat tgcgcgaaga acaaggccgt ctgaacatcc ttttgggcga tgaaaacgaa
    18241 aagcgcaagc tgattatcaa agagatgcag gcggatatga aacaatacgg cgatgcacgc
    18301 cgcacgctgg tggaagaggc cggacgcgcc gtgctgacac aaaccaccgc cgacgaaccc
    18361 atcacactga ttctgtcgga aaaaggctgg atacgcagcc gtgccggaca caatctcgat
    18421 ttgagccaaa ccgcgttcaa agaaggcgac cgcctcaaac aaacccttga aggcagaacg
    18481 gttttacccg tcgtcatcct cgattcattg ggcagaacct atacactcga tgccgccgaa
    18541 attcccggcg gacgcggcga cggcgtgccg gtttcatcct tgatcgagtt gcaaaacggc
    18601 gcgaaacccg ttgcgatgtt gacaggattg ccggaacaac attatttatt atcaagcagc
    18661 ggcggctacg gcttcatcgc caagttggct gatatggtcg gacgcgtaaa agcgggcaaa
    18721 gtggtgatga ccacagacag tggcgaaacc gtcctgccgc cgattgccgt ctatgcctcc
    18781 tcgttcatca accccgactg caaaatcatt gccgccacca gtcaaaaccg cgccctcgcc
    18841 ttccccatcg gcgaattgaa aattatggcg aaaggcaaag ggctgcaaat catcggatta
    18901 aacgccggcg aatcgctgac gcataccgcc gtttcttccg agccggaaat cctgattgaa
    18961 agcgaaggca ggcgcggcgc ggcgcacaaa gaccgcatcc ccgtctccct gcttgaggca
    19021 aaacgcggca aaaaaggcaa actattaccc atatcgggca gcctgaaaca gctttcttcc
    19081 cccaaataaa cccgattcca cacataatta tggtgatttc caacccccgc gaacttgaaa
    19141 aactcaaaga ccggattccc aatctgatca acatcatccg cgtcgccatc gtttttccgc
    19201 tgatgattat gcacatcctc gggctggaaa ccggcagccg tgcgaacctg cacgcttcgt
    19261 ggacggcgtg ggcgttttat gtttggctcg ccattgcctg ctggctgatt ttcttttcca
    19321 tcatccatcc gcattggcaa tggcagtcgc tgaaaatgcc gcgtttcagc gcggtggcgg
    19381 acatcacgat gatcggcgtg ctgacctacc tgtttggcgg catcgattcc ggcttcggca
    19441 tcctgatcct gcccttcgtc gtctgctcct gcctgctcag ctacgggcgt taccccctgc
    19501 tctattccag ctacgccgcc atcctgctga tattcaacgc cattgccgac ggcgatatcg
    19561 gcaaataccc gctcatatcg gatgcccgaa ccgcctcggt aaccttcatc cttgtcgccg
    19621 cctcctatct ttccgccatc ttcacctcac tgtcggtcag atacatcgac agcgccggcc
    19681 ggctggcacg cgacaaccac ctcgcctacc ggcgcatcaa aggcttgagc caaaccgtac
    19741 tcaaccgcgt tcaggaagcg gtcgtcgtca tcaacgtcga gcatcagacc atactgttca
    19801 ataaaaaggc aaaagacctg ctacccatgc ttgaaatcgg acagcatact gccctgttcg
    19861 accctattac cgttttatgg gacaaggcct cttcgcgtac tttcgaacgc aatatcgaca
    19921 cgcccggcct gaacgccggc atccgcgccg ttccggtcaa caaagggccg gacaagctgc
    19981 tcatcctcta catccgcccg caaagcgaaa ttcaggcaga agccctgtcc gtcaaacttg
    20041 ccgcgctcgg acaactgacc gccaacctcg cccacgaaat ccgcaacccg atgtccgcca
    20101 tccgccacgc caacgacctg ctgcgcgaaa atatggaagc gggggcggca gatccgttca
    20161 acgccaaatt gtgcaaaatc atcgacggca acgtctgccg tatcgacaaa atgctcgaag
    20221 atatttcctc gccaacaagc gcaacaaaac cgaacgcgaa accatcggcc tgataccgtt
    20281 ttgggaagaa ttcaaacaag agttcctgct cggccatccc gatgccgccg gctgcatccg
    20341 cctagatatg cagggcaatc acctgactgc ctatttcgat cctgcccacc tgcggcaaat
    20401 tatgtggaac ctcgccaaca acgcgtggcg gcacagcagc aaacgtaacg gctcgattgc
    20461 cgtcgtcgcc cgtcctgcac aaaaaaacac catcagcatc ctgtttgccg acgacggcgg
    20521 cggcgttccg cccgaagtgc aggaacactt gttcgaaccc ttttacacca cagcaaaaaa
    20581 cggaaccggc ttgggactgt atgtcgcccg cgaactggca cacgccaatt tcggcgattt
    20641 gacctacctg ccggaagcca aatgtttcga actcacatta ccggaaaaaa ccaatgactg
    20701 aactgcaaca tcccgtcctc gtcgtcgatg acgaaaccga cattctcgac ctgatggaaa
    20761 tgaccctgat gaaaatgggc ttgcgcgtcc ataccgcgtc aggcgttgcc gaagccaaaa
    20821 acaagctcga cagccaacgc tattcgctcg tcctgaccga tatgcgtatg ccggacggct
    20881 cggggctaga agtcgtccaa cacatcaaca gccgcctgct cgatacgccg gttgccgtca
    20941 tcaccgcctt cggcaatgcc gatcaggcac aggaagccct gcgttgcggc gcgttcgacc
    21001 ccgataccat gcagatacag gactatctcg accaaatcga acgcgacatc atcgaacaaa
    21061 ccctcaaaca aaccgaaggc aaccgcacgc aggccgacaa acgcttgggc atcagcttcc
    21121 gttccatgcg ctaccgtatg gaacgcctca acatcggctg acgacaaaac ggcatccgca
    21181 ccatctccgc ccacccgaaa aaatgccgtc tgaaacggca cgggaaagcg ggttcgcccc
    21241 acgcccgaac ggacacaaaa caccatgacc gacatcctta ttgacaacac cgccaccgaa
    21301 accgtccgca ccctgatacg ggcattcccc cttgtgcccg tttcccaacc gcccgaacaa
    21361 ggcagctacc tccttaccga acacgatacc gtcagcctca ggcttgtcgg ggaaaaaagc
    21421 agcgtcatcg tcgactttgc ctcgggagcc gcacaatacc ggcgcacaaa aggcggagga
    21481 gaactcatcg ccaaagccgt caaccatacc gccaaaccca ccgtatggga tgcaaccgca
    21541 ggattggggc gcgacagctt cgtcctcgcc tcgctcggac tgaccgttac cgccttcgag
    21601 cagcatcccg ccgtcgcctg cctgctttca gacggcatcc gccgcgccct cctcaatccc
    21661 gaaacgcaag acaccgccgc gcgcatcaac ctccatttcg gcaacgccgc cgaacagatg
    21721 cccgcccttg ttcaaacaca aggcaaaccc gacatcgtct atctcgaccc catgtatccc
    21781 gaacgccgca aaagtgccgc cgtcaaaaaa gaaatggcct acttccaccg gctcgtcggc
    21841 gaggcgcaag atgaggccgt cctcctccat accgcacgcc aaacggcaaa aaaacgcgtc
    21901 gtcgtcaaac gcccccgcct cggcgaacac cttgccggac aagcccctgc ctaccaatac
    21961 acaggcaaaa gcacccgctt cgacgtttac ctgccctacg aagcggacaa gggataacgc
    22021 ccataaaaca agacaccgaa aaatttgccg ttcttatgca aacgagaaac cggtttttgc
    22081 gtttcgactg ttttggataa atcatcacac cttaaagttt gtcattccca cggaagtggg
    22141 aatctagaaa tgaaaggcaa caggaattta tcgtaaatga ctgaaaccga acggactaga
    22201 ttcccgcctg cgcgggaatg acgatgtaaa attatccggg attcaaaaag acagactttc
    22261 gcgtccgtgg gaatgactgc ggaaagatga tttttatttt gtcataaaaa tccgcacctt
    22321 aatcagttgg cggttaaatc aaacttttag ggtgcagatt actttttatg atttcagacg
    22381 gcattttgac aggccgcaaa cctatttcgg caataccaaa aacttaatca gcagttcttt
    22441 gaatacaaaa ccgaacacgc ccaagcccaa aaccaaaaac aaaatggcga tgccgaattt
    22501 gcctgctttg gactccttgc ccaaattcca aacgataaaa cccaaaaaaa taatcaagcc
    22561 ggtcaggcag attttcaacg cccaatcggc aaaaaccgct tcatccatat ttttttccta
    22621 ttgttgatgt gtatgccata taagataagg gtttcagacg gcatctgctg tccaatgccg
    22681 tctgaaacac gcaatcagcg tgcgagtgcc tgtttcaaat cgtcaatcaa atcgccaaca
    22741 tattccaaac cgaccgacag gcgcaccaat ccggggcgga tgttggcggc gagtttttct
    22801 tcgggctgca tcctgccgtg cgtggttgtc cacggatggg tgatggtcga gcgcacgtcg
    22861 ccgaggttgg cggtacggga aaagagttcc acgccgtcca cgactttcca cgcggctgct
    22921 tggtcggcga cttcaaagcc gatgacgatg ccgccgccgt tttgctgttt gcggataagc
    22981 gccgcctgcg gatggtcggg caatccggtg tagtacacgg cttgaacctg cggctgcgct
    23041 tgcagccatt gtgcgatttt cagggcgttg tcgaactgtt tttccatacg cagcgacagg
    23101 gtttccacgc cgctcaacaa ctgccacgca ttaaacggcg acatcgccag cccgcaagag
    23161 ttgcagtaaa cggcggcctg cgccatcaat tcttccgaac ccgccaacac gccgcccatc
    23221 acgcgtccgt gtccgtctat ggctttggtc gcggaggaaa cggaaatatc cgcgccgtgt
    23281 ttcaaaggct gcgagccgac gggcgacagc aggctgttgt ccaccaccaa aatcgcgccg
    23341 atgccgtgcg ccaattccgc caaggcttcc aagtcggcca cttcgcccaa gggattggac
    23401 ggcgtttcca aaaacagcag tttggtgttg gctttgacgg cggctttcca ttcgtttata
    23461 tcagtcggcg acacgtggct cacttcgatg ccgaatttgg taacgatgtt attgataaag
    23521 ccgacggtcg tgccgaacag gctgcggctg gaaaccacgt ggtcgcccgc ctgcaaaaag
    23581 gtgaaaaacg ccgcctgaat cgcagacata cccgtcgaag tggcgaccgc gcgttccgca
    23641 ccttccaaag cggcgatgcg tttttcaaag gcggctgtgg tcgggttggc ggtacgggta
    23701 taagtgaacc ctttgatttt ttttgaaaac aaatcggcag cgtgttgggc gttgtcccac
    23761 atgaagctgc tggtcagaaa caatgcctga ttgtgttcgc ggtattcggt ttgttctttg
    23821 ccgccgcgta tggcgagcgt ttgcggatgg agttttttgc tcatcggtga ttcctcggtt
    23881 ttgtccgttc ggcaacggag cgtgcgcccg ttgtttaatt tgttaatatt ttgcgcctgt
    23941 tctatgatgc tttcaagtcg gatgagaatg caaatgccgt ctgaaacggc tttcagacgg
    24001 catggcaatc agcgtttgta ttttaactcg tacttgatgt cgttgaggat tttgcggaca
    24061 tcgtgttcca acacgtcttc gactaccgcc cccgcctgct cgtgcagcat ctgctggagc
    24121 tgataggtga aaaccgccat ctgcttttgc accgccgttc ggatgatgcc gttgacggta
    24181 tcggtcagat gcgggcgcag gcgtttgatc agccgttcgg tcagctcctg ttcggacagg
    24241 cagaacactt cgcgccggtt gacggctttc gggttcagga tattgatttg gacgggcatc
    24301 aacgcctctt ccgcaccgcc ttcgccgttt tccgcaaccc cgtctgccgc cgtacccgat
    24361 tctgccgcat cggcgttttc cctgccttca atctgtccgg tttcaaattc ggcactgtct
    24421 tttttggcat cgaaccggat tctccgccgc gattcgatgt gtttttccga aaccgacatt
    24481 tgcagggaag cctgcgcgtt gagccagttt tcctgaagga caatcatcgg gtcggtttcg
    24541 acttcctcgc cgcaatcggc aacggcggca ttgtgttcct cctgccattt tttcagatac
    24601 gccttcaaca cacgggctcg gctctcatcg tccagtttcg gcacaggcgc gtccgttccg
    24661 gtttcagagg ggcgggacag cggcgcgtaa gtcggcactg ccttcatacg gcgcgtctga
    24721 cgcaggtttt ccaaacgttt ttcccaattc ggctctttat tcgcatccat tttcggcttc
    24781 cggttcttaa tctttgcaag cagacaaacc cgcgcccaaa gcgcggtttg atataatggc
    24841 gcattttaac agattcgcga ggatacatca tgggcagcat cgaacagcgt ttggaatatc
    24901 tggaagaggc gaacgacgtg ctgcgtatgc agaaccacgt cctgtccacc gcattcaaag
    24961 ccttaatccg cgcccttccc gccgaaaccg ccgaaatcgc ggtcgagtcg attcagcttg
    25021 cttttgagga cgccttggca gaattgagct atgaggacag cccccatacc gatttgttcc
    25081 acgacgttac ttatgcgttt ttccgtgaaa aagaacgtta attttatgtt aaactgattt
    25141 tttaggcttt ttgattaccg aaaggaattt tgatgaatat gaaaaaatgg attgccgccg
    25201 cccttgcctg ttccgcgctt gcgctgtctg cctgcggcgg tcagggtaaa gatgccgccg
    25261 cgcccgccgc aaatcccgac aaagtgtacc gcgtggcttc caacgccgag tttgccccct
    25321 ttgaatcttt agactcgaaa ggcaatgtcg aaggtttcga tgtggatttg atgaacgcga
    25381 tggcgaaggc gggcaatttt aaaatcgaat tcaaacacca gccgtgggac agccttttcc
    25441 ccgccttgaa caacggcgat gcggacgttg tgatgtcggg cgtaaccatt accgacgacc
    25501 gcaaacagtc tatggacttc agcgacccgt attttgaaat cacccaagtc gtcctcgttc
    25561 cgaaaggcaa aaaaatatct tcttccgacg atttgaaaaa catgaacaaa gtcggcgtgg
    25621 taaccggcta cacgggcgat ttctccgtat ccaaactctt gggcaacgac aatccgaaaa
    25681 tcgcgcgctt tgaaaacgtt cccctgatta tcaaagaact ggaaaacggc ggcttggatt
    25741 ccgtggtcag cgacagcgcg gtcatcgcca attatgtgaa aaacaatccg accaaaggga
    25801 tggacttcgt taccctgccc gacttcacca ccgaacacta cggcatcgcg gtacgcaaag
    25861 gcgacgaagc aaccgtcaaa atgctgaacg atgcgttgga aaaagtacgc gaaagcggcg
    25921 aatacgacaa gatttacgcc aaatattttg caaaagaaga cgaacaggcc gcaaaataag
    25981 cccgcccgtc cgaacacaat gccgtctgaa gccctttcag acggcattgt tcatcaatcg
    26041 gcctacaatg aactgcctgc tgatttctcc ctaccgcaaa gcaacaggca aagattacaa
    26101 atatcaaaat ccgagtaaaa cagtatttta ttaaaacaaa ttgataatca agagattaga
    26161 attatgtatt gtctttaccg tacaaacgct ggcactattt caacctgata aaaaacagcc
    26221 ttcaaaaagg ttgcttaaaa cagcagcaga cacttaccgc cacaaccttg aaaaggaaca
    26281 caatcatgac cgtcatcaaa caggaagact ttatccaaag catttgcgat gccttccaat
    26341 tcatcagcta ctaccatccc aaagactaca tcgacgcgct ctataaagcg tggcagaaag
    26401 aagaaaaccc cgccgccaaa gacgcgatga cgcagatttt ggtcaacagc cgcatgtgtg
    26461 ccgaaaacaa ccgccccatc tgccaagata ccggtatcgc gaccgtcttc ctcaaagtcg
    26521 gtatggatgt gcagtgggat gcggacatga gcgtcgaaga aatggttaac gaaggcgtgc
    26581 gccgcgccta cacttgggaa ggcaatacgc tgcgcgcttc cgttctcgcc gaccccgccg
    26641 gcaaacgcca aaataccaaa gacaacacgc ccgccgtcat ccatatgagc atcgtgccgg
    26701 gcgacaaagt cgaagtaacc tgcgcggcaa aaggcggcgg ttctgaaaac aaatccaaac
    26761 tcgccatgct caatccttcc gacaacatcg tcgattgggt attgaaaacc atcccgacga
    26821 tgggcgcggg ctggtgtcct cccggcatct tgggcatcgg catcggcggc acgcctgaaa
    26881 aagccgtgtt gatggcaaaa gagtccctga tgagccacat cgacatccaa gaattgcagg
    26941 aaaaagccgc gtccggcgcg gaattgtcca ccaccgaagc cctgcgcctc gaactctttg
    27001 aaaaagtcaa cgcgctgggc atcggcgcac aaggtttggg cggtctgacc accgtgttgg
    27061 acgtgaaaat cctcgattac ccgacccacg ccgcctccaa accgattgcc atgattccga
    27121 actgcgccgc cacccgccac gtcgaatttg aattggacgg ctcaggccct gtcgaactca
    27181 cgccgccgcg cgtcgaagac tggcccgatt tgacttacag ccccgacaac ggcaaacgcg
    27241 tcgatgtcga caagctgacc aaagaagaag tggcaagctg gaaaaccggc gacgtattgc
    27301 tgttgaacgg caaaatcctc accggccgcg atgccgcaca caaacgcctc gtcgatatgc
    27361 tcaacaaagg cgaagaatta cccgtcgatt tcaccaaccg cctgatttac tacgtcggcc
    27421 ccgtcgatcc ggtcggcgat gaagtcgtcg gtccggcagg tccgaccaca gccacccgca
    27481 tggacaaatt cacccgccaa atgctcgaac aaaccggcct cttgggcatg atcggcaaat
    27541 ccgaacgcgg cgcggcggcg tgcgaagcca tcgccgacaa caaagccgtg tacctgatgg
    27601 cggtcggcgg cgcggcatac ctcgtggcaa aagccatcaa atcttccaaa gtcttggcgt
    27661 tccccgaatt gggtatggaa gccatttacg aatttgaagt caaagatatg cccgtaaccg
    27721 tcgccgtaga cagcaaaggc gaatccatcc acgccaccgc cccgccccaa tggcaggcga
    27781 aaatcggcat catccccgtc aaatcttgag gcgtcatgcc gtctgaacac aaaatctgct
    27841 ttcagacggc atttccgccc ccggttgcgg tacaatccac catttcatca ctcggcgacc
    27901 cacaccgtga aaatcctcat tttaggcaac ggacaggtag gttctaccgt cgcacaaaac
    27961 cttgccgcca tacccaacaa cgacgtaacc gttatcgaca tcgacgaaaa agcattgcag
    28021 gaaacaggca gccgcctcga tgtccaaacc gttttcggca acggcgcatc ccccttcaca
    28081 ttagaacgcg ccggcgcgga agatgccgac ttgctgctcg cgctctcccg cagcgacgaa
    28141 accaacatcg tcgcctgcaa agttgccgcc gacctgttca acatccccgg ccgcatcgcg
    28201 cgcgtccgtt ccagcgaata cctcgaatac ctcagcccca agctcgaaaa caacgaaaac
    28261 ggcagccttt ccatattcgg cataaccgaa accatcagcc ccgaacagct cgttaccgaa
    28321 cagcttgccg gcctgataga ctgcccgggc gcattgcagg ttttacgttt tgcagacgac
    28381 cgcgtgcgga tggtcatcat acaggcgcgg cgcggcgggt tgcttgtcgg acgcagcatt
    28441 gccgacatcg cccaagattt gcccgacggg gccgactgcc aaatctgcgc cgtttaccgc
    28501 aacaaccgcc tcatcgtccc cgcgccgcaa accgtcatca tcgaaggcga cgaaatccta
    28561 tttgccgccg ccgccgaaaa catcggcgcg gtcatacccg aattgcgccc caaagaaacc
    28621 agcacccgcc gcatcatgat tgccggcggc ggcaacatcg gctaccgtct cgccaagcag
    28681 ctcgaacacg catacaacgt caaaatcatc gaatgccggc cgcgccgtgc cgaatggata
    28741 gccgaaaacc tcgacaacac cctcgtcctg caaggttcgg caaccgacga aaccctgctc
    28801 gacaacgaat acatcgacga aatcgacgta ttctgcgccc tgaccaacga cgacgaaagc
    28861 aacattatgt ccgccctttt ggcgaaaaac ctcggagcga agcgcgttat cggcatcgtc
    28921 aaccgctcaa gctacgtcga tttgctcgaa ggcaacaaaa tcgacatcgt cgtctccccc
    28981 cacctcatca ccatcggctc gatactcgcc cacatccggc gcggcgacat cgttgccgtc
    29041 caccccatcc ggcgcggcac ggcggaagcc atcgaagtcg tcgcacacgg cgacaaaaaa
    29101 acttccgcca tcatcggcag gcgcatcagc ggcatcaaat ggcccgaagg ctgccacatt
    29161 gccgccgtcg tccgcgccga aaccggcgaa accattatgg gacaccatac cgaaaccgtc
    29221 atccaagacg gcgaccacat catctttttc gtctcgcgcc ggcgcatcct gaacgaactg
    29281 gaaaaactca tccaggtcaa aatgggcttt ttcggataaa ccgccccatt ccggacacat
    29341 tgccgccaag cggtatggaa gcggaaataa tggtaggtgg gcttcagacg gcatccgccc
    29401 tccccgtcct tcccgcgtaa gcgggaatcc agaccttggg atagcggcaa tattcaaagg
    29461 ttatctgaaa atttagaggt tctagattcc cgtttccacg ggaaggacga agagttgcgg
    29521 gaatctagac cattggaaag cggcaatatt caaaggttag ctgaagcttt agaggttcta
    29581 gattcccgtt tccacgggaa tgacgaaagg tggcgggaat ccaaaccatt gggcaacagc
    29641 aatattcaaa gattatctga aagtttgagg ttctagattc ccgctttcgc gggaatgacg
    29701 aaaagtggcg ggaatgacga agtttcagac ggcatcgccc gcctgttttg atatggcggc
    29761 acccccccga caaaaaaaac aatccggaac gcatctgacc gttccggctt gttttcaggc
    29821 gaatccgccg catcagaaca tactgcgcac gcccatattg acctgccaag tctggcgcat
    29881 cgtgtgcatc gaagaccttt gcgcctcaaa ataaagctgc cttccgttgt cggcattacc
    29941 acgcaaaaaa aatgaattgc ttgatattcc aatgtttttt atatgttttt atattgtgat
    30001 gcgatcagac aaacgccccc ctgacatttg tttagacggc atcgtattgc taaatttcta
    30061 taagtatgta taatgtccgt ttccacgcgc ccatcgtcta gaggcctagg acactgccct
    30121 ttcacggcgg caaccggggt tcgaatcccc gtgggcgtgc caattcaaaa acctgcttgt
    30181 ttcaagcagg tttttttatt atgaatcgtc attcccgcac ccttcgtcat tcccgcaaaa
    30241 gcgggaatct agagcgtagg gttgaagaaa ccgttttatc cgataagttt ccgtgccgac
    30301 aggtctggat tcccactttc gtgggaatga cgagtctttt ataaccttta aggctgctct
    30361 gcccaatggt ttggattccc gcttatgcgg gaatgacggg gcggggcgat gccgtctgaa
    30421 acttcgtcat tcccacaacc cttcgtcatt cccgtgaaaa cgggaatcta g
//
LOCUS       DAWHSK010000004        27265 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.004, whole genome shotgun sequence.
ACCESSION   DAWHSK010000004 DAWHSK010000000
VERSION     DAWHSK010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 27265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27265
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.004"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(178..1953)
                     /gene="ptsP"
                     /locus_tag="WIC03_000101"
     CDS             complement(178..1953)
                     /gene="ptsP"
                     /locus_tag="WIC03_000101"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEZ3882669.1"
                     /translation="MSIVLHGVAAGKGIAVGCAHLIARGTEEVPQYDVAEADTDAEAE
                     RFDAAVKATRKELEQLRSAIPENAPTELGAFISLHLMLLTDVTLSREPVDILREQKIN
                     AEWALKQQSDKLAAQFDNMDDAYLRERKQDMLQVVRRIHNNLIGQGNELEVADNLFDE
                     TVLIANDLSPADTVLFKEQRIAAFVTDAGGPTGHTAILGRSLDIPSVVGLHNARKLIT
                     EGETVIVDGINGVLIIAPDESVLNEYRRRAREYRSHKRDLNKLKKTAAATADGVCIEL
                     VGNIESAEDVKPLHNLGADGIGLFRSEFLYLNRDTMPSEDEQYEVYSAIVKKMKGKSV
                     TIRTVDLGVDKNPRWFGKNSTPNGSLNPALGMTGIRLCLAEPVMFRTQMRAILRAAVH
                     GPVRMMWPMITSVSEVRQCLIHLDTAQRQLAERGDAFGKVGIGCMIEIPSAALTVGSI
                     LKLVDFISVGTNDLIQYILSVDRGDDSVSHLYQPGHPAVLKMLQHVIRTANRMDKDVS
                     VCGEMAGDTAFTRVLLGMGLRRFSMNPNNILPVKNIILHSNVGQLESDIAKVIRCEDE
                     EKSEKLIKQMNSVSVEEEADFKGRK"
     gene            complement(1953..2222)
                     /locus_tag="WIC03_000102"
     CDS             complement(1953..2222)
                     /locus_tag="WIC03_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEZ3882670.1"
                     /translation="MLKQSIEIINKLGLHARASNKFTQTASQFKSEVWVTKNDSRVNG
                     KSIMGLMMLAAAKGTVIELETDGADEAEAMRALTDLINGYFGEGE"
     gene            complement(2291..2728)
                     /locus_tag="WIC03_000103"
     CDS             complement(2291..2728)
                     /locus_tag="WIC03_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEZ3882671.1"
                     /translation="MIGLLIITHETIGEAYRKLAHHFFPGGLPENVRILGVQPTEDQD
                     DIINNAIAALQEFPDNDGVLIMTDIFGATPCNAARRLVRENKSAILTGLNAPMMVKAV
                     QYSPAAEDLAAFTECVREAAVKGIFAITSAPEDLVCRRSGDAV"
     gene            complement(2809..3372)
                     /locus_tag="WIC03_000104"
     CDS             complement(2809..3372)
                     /locus_tag="WIC03_000104"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HEZ3882672.1"
                     /translation="MTDLETKRLETQAMLENADLLFDQGQCRAALQKVADEITRDLGG
                     KYPLLLPVMGGAVVFTGQLLPLLRFPLDFDYVHVSRYGDKLEGGAFNWKRMPDAEQIR
                     GRHVVVLDDILDEGHTMSAIQAKLLEMGAASCRAAVFANKLIDKPKPIRADYVGLDVP
                     NRYVFGYGMDAAGCWRNLGEIYALDGK"
     gene            complement(3441..4265)
                     /locus_tag="WIC03_000105"
     CDS             complement(3441..4265)
                     /locus_tag="WIC03_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HEZ3882673.1"
                     /translation="MIKKTIGGIIPIFTAVFIPASAGAADLMLAQEYKGQEIAGWAMS
                     EKLDGVRAYWDGKHLISRQGYAFTPPKGFTAQFPPYPLDGELYSGRGQFEQISATVRS
                     VSSDWRGIRLHVFDVPKAQGNLYQRLAVATQWLKTHPNAPITIIPQIKVRVRQHAMDF
                     LKQIEAQGGEGVMLRQPESRYSGGRSSQLLKLKSQYDDECTVTRHYEGKGRNAGRLGA
                     VGCKNRHGEFRIGSGFKDKDRDNPPKIGTLITYRYRGFTQKGTPKFATFVRVRTDR"
     gene            4357..4989
                     /locus_tag="WIC03_000106"
     CDS             4357..4989
                     /locus_tag="WIC03_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEZ3882674.1"
                     /translation="MTLRYEIFPVTPFRQNCTLIWDDESGEAVLTDVGGDVPFLLQAL
                     ANRKLTLTAIWLTHGHLDHTGGVVEMLKTHKVPVLGPHPDDEFLLQSLPQTTAQYGFP
                     VSPAFAPNRWLEEGETLKVGRYAFQVLHIPGHTPGHVVFYCAEAELLIAGDVLFYETI
                     GRTDFPRGNHADLINNIRNKLFALPETVQVVAGHGRMTSIGHEKRHNPFF"
     gene            complement(5195..6979)
                     /gene="rmuC"
                     /locus_tag="WIC03_000107"
     CDS             complement(5195..6979)
                     /gene="rmuC"
                     /locus_tag="WIC03_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEZ3882675.1"
                     /translation="MELMTVLLPLAALVSGVLFTWLLMKGRFQGEFADLNAQLAEKAA
                     RCDFVEQAHAEIASELAVLDGKYRHLQDENYALGNRFSAAEKQIAHLQEKEAESARLK
                     QSYIELQEKAQGLAVENERLATQLGQERKAFADQYALERQIRQRIETDLEESRQTVRD
                     VQNDLADVGNRFAAAEKQIAHLQEKEAEAERLRQSHTELQEKAQGLAVENERLATQIE
                     QERLASEEKLSLLGEARKSLSDQFQNLANTILEEKSRRFTEQNREQLHQVLNPLNERI
                     HGFGELVKQTYDKESRERLTLENELKRLQGLNAQLHSEAKALTNALTGTQNKVQGNWG
                     EMILETVLENSGLQKGREYTVQAASVRKEEDGGTRRLQPDVLVNLPDNKQIVIDSKVS
                     LTAYVRYTQAADADTAARELAAHVASIRAHMKGLSLKDYTDLEGVNTLDFVFMFIPVE
                     PAYLLALQHDAGLFQECFDKRIMLVGPSTLLATLRTVANIWRNEQQNQNALAIADEGG
                     KLYDKFVGFVQTLESVGKGIDQAQNSFQTAFKQLAEGRGNLVGRAEKLRLLGVKANKQ
                     LARDLTERANETTALSESLEYAAEDEAV"
     gene            complement(7318..8106)
                     /locus_tag="WIC03_000108"
     CDS             complement(7318..8106)
                     /locus_tag="WIC03_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HEZ3882676.1"
                     /translation="MKQAMKNWFAALLLAVPMGAAFASGGHAHYEKVDIDLRDQVSLQ
                     RGAQIFTNYCLSCHSASGMRFNRLKDIGLTDEEIKKNLMFTTDNVGDVMHSAMNPKDA
                     AKWFGAAPPDLTLIARSKGADYLYAYMRGFYKDPTRPSGWNNTVFDKVGMPHPLWEQQ
                     GVQAVELDAKGQPVMVKDEHGEMKPKLYWESTGLHSRRLPNGKVIQKEYDAYVRDLVN
                     YLVYMGEPAQLQRKRIGYVVMIFLFAVMLPLAYFLKKEYWKDVH"
     gene            complement(8121..9470)
                     /locus_tag="WIC03_000109"
     CDS             complement(8121..9470)
                     /locus_tag="WIC03_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HEZ3882677.1"
                     /translation="MANQTNSKAKALLGWMDARFPLSKMWNEHLAQYYAPKNFNFWYY
                     FGSLALLVLVIQIVSGIFLTMNYKPDGNLNAYHLPAAFTAVEYIMRDVSGGWIIRYMH
                     STGASFFFIVVYLHMFRGLIYGSYKKPRELVWIFGSLIFLALMAEAFMGYLLPWGQMS
                     FWGAQVIINLFSAIPVIGPDLSTWIRGDFNVSDVTLNRFFALHVIAVPLVLLGLVVAH
                     IIALHEVGSNNPDGVEIKKNKDENGIPRDGIPFHPYYTVKDILGVVVFLIVFCAVLFF
                     APEGGGYFLEAPNFDAANALKTPPHIAPVWYFTPFYAILRAIPSFLGTQVWGVIGMGA
                     AVVLIALLPWLDRGEVKSVRYRGPIFKTALVLFIISFIGLGILGAMVATDTRTLVARI
                     LSFVYFAFFLGMPFYTKLDKNKPVPERVTMSTAKQKIMFFVYVGITAVGAYLFATNI"
     gene            complement(9489..10070)
                     /gene="petA"
                     /locus_tag="WIC03_000110"
     CDS             complement(9489..10070)
                     /gene="petA"
                     /locus_tag="WIC03_000110"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEZ3882678.1"
                     /translation="MDNQEINNGRRRFLTLATCGAGGVAALGVATPFVASFFPSEKAK
                     AAGAAVEVDVSKIEAGQLLTAEWQGKPIWVLNRTDQQLKDLKGLNGELTDPNSDAEQQ
                     PEYAKNETRSIKPNILVAIGICTHLGCSPTFRPDIAPADLGADWKGGFFCPCHGSKFD
                     LAGRVYKGVPAPTNLVVPPYKYLSDTTILVGED"
     gene            complement(10193..10942)
                     /locus_tag="WIC03_000111"
     CDS             complement(10193..10942)
                     /locus_tag="WIC03_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEZ3882679.1"
                     /translation="MVLRRDFLTWCNETLQTALFKDYAPNGLQVEGREYIGKIVTSVT
                     ASRAAIDFAVEQKADLLLVHHGMFWKNELPTVTGWKKERIAALLRHDINMAGYHLPLD
                     AHPTLGNNAQLADRLGFATEKRFGEQNLLNSGSLKQAKTLGALAAHIETVLQRKPVVI
                     GKPEREIRRVAWCSGGAQGFFQTAIDEGVDLYLTGEISEAQYHLANETGTAFISAGHH
                     ATERYGVRALAESAAEVFGLEVCHFDENNPA"
     gene            11073..12002
                     /locus_tag="WIC03_000112"
     CDS             11073..12002
                     /locus_tag="WIC03_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEZ3882680.1"
                     /translation="MDSIIELRHLKTLLALEETGSVSLAAKRVFLTQSALSHQIRMLE
                     NHYGAPLFERKSTPLRFTPVGERLLRLAHELIPQVAVAERDLARITEGEAGELRIAVE
                     CHTCFDWLMPAMGEFRPMWPQVELDIVSGFQADPVGLLLQHRADLAIVSEAEKQNGIS
                     FQPLFAYEMVGICAPDHPLAAKNVWTAEDFIGETLITYPVPDEMLDLPKKILIPKNIN
                     PPRRHSELTIAIIQLVASRRGIAALPYWTVMPYLEKGYVVHRQITDDGLQSKLYAAIR
                     TEDTDKSYLNNFCQIIRERGFADLPGLSELEPV"
     gene            complement(12257..12649)
                     /gene="rpsI"
                     /locus_tag="WIC03_000113"
     CDS             complement(12257..12649)
                     /gene="rpsI"
                     /locus_tag="WIC03_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEZ3882681.1"
                     /translation="MNGKYYYGTGRRKSSVARVFLIKGTGQIIVNGRPVDEFFARETS
                     RMVVRQPLVLTENAESFDIKVNVVGGGETGQSGAIRHGITRALIDFDAALKPALSQAG
                     FVTRDAREVERKKPGLRKARRAKQFSKR"
     gene            complement(12659..13090)
                     /gene="rplM"
                     /locus_tag="WIC03_000114"
     CDS             complement(12659..13090)
                     /gene="rplM"
                     /locus_tag="WIC03_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEZ3882682.1"
                     /translation="MKTFSAKPHEVKREWFVIDAQDKVLGRVAAEVASRLRGKHKPEY
                     TPHVDTGDYIIVINADKLRVTGAKFEDKKYFRHSGFPGGIYERTFREMQEQFPGRALE
                     QAVKGMLPKGPLGYAMIKKLKVYAGAEHAHAAQQPKVLELK"
     gene            complement(13268..13447)
                     /gene="ssrS"
                     /locus_tag="WIC03_000115"
     ncRNA           complement(13268..13447)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="WIC03_000115"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(13453..13758)
                     /locus_tag="WIC03_000116"
     CDS             complement(13453..13758)
                     /locus_tag="WIC03_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEZ3882683.1"
                     /translation="MNIEQVHIEVMHARLTVNTPAEEKDTLLQAVGMLNGKAEAIREG
                     GRVADSEKIVIMAALNVVHDLLKTSLNGGDLAIGDFARKIADMDNACQKALSRLAQE"
     gene            complement(13769..14098)
                     /locus_tag="WIC03_000117"
     CDS             complement(13769..14098)
                     /locus_tag="WIC03_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3882684.1"
                     /translation="MKQNIEKLESSVYTLVQKFETLVSENRRLKETVAELKRAHERQK
                     LEHETAVDELSEALLVQVGKLKEDLQNKIDSLTEENTRYRSLLEQSREKISALAARLP
                     QWQETQQ"
     gene            complement(14153..15142)
                     /locus_tag="WIC03_000118"
     CDS             complement(14153..15142)
                     /locus_tag="WIC03_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEZ3882685.1"
                     /translation="MKITVIGAGSWGTALALHFSQHGNRVSLWTRNADQVRQMQEARE
                     NKRGLPGFSFPETLEVCADLADALKDSGLVLIVTSVAGLRSSAELLKQYGAGHLPVLA
                     ACKGFEQDTGLLTFQVLKEVLPDNKKIGVLSGPSFAQELAKQLPCAVVLASENQEWIE
                     ELVPQLNTTVMRLYGSTDVIGVAVGGAVKNVMAIATGLSDGLEYGLNARAALVTRGLA
                     EITRLASAMGAQPKTMMGLAGIGDLILTCTGALSRNRRVGLGLAEGKELHQVLVEIGH
                     VSEGVSTIEEVFNTACKYQIDMPITQTLLQLIRKEMTPQQVVERLMERSARFE"
     gene            complement(15265..17967)
                     /gene="ppc"
                     /locus_tag="WIC03_000119"
     CDS             complement(15265..17967)
                     /gene="ppc"
                     /locus_tag="WIC03_000119"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEZ3882686.1"
                     /translation="MQLHILNNPKDAALAADAEFLKQSLFNLLHEEASPLVVETVKLL
                     STSDDSAALIEKVLPQLDEQQTHDLTLACGLFAQILNIAEDVHHERRRQIHEEAGRGG
                     AEGSLTETVRRLKAGKADGKSVQRQLDNTSVTAVLTAHPTEVQRQTVLSFNRRIRALL
                     PQRERCTNADALARLRREIDTILLGLWQTSETRRHKLSVNDEINNGVSIFPMSFFEAL
                     PKLYRNMEHDFQMVYPDVCVPNILKIGGWIGGDRDGNPFVSAETLRFAFRRHADAVFR
                     FYRGELDKLYRELPLSIRRVKVNDDVMALAALSPDEELARTEEPYRRAIAYIMARAMG
                     KARALGLGMGCKFGFLEPYASAQEFLDDLKKLQRSLIDNGSRLLAEGRLADLIRSVSV
                     FGFHMMPLDLRQHAGKHADVVAELFQHAGLEDYNSLNEEQKQAALLRELSHQRPLYSP
                     FITYSDHTRHELAIFNEARKIKDEFGEDAVTQSIISNCEQPSDLLALALLLKESGLLA
                     VENGKPQSRINIVPLFETIEALENACPVMETMFRLDWYDALLESRGNIQEIMLGYSDS
                     NKDGGYVTSSWCLYQAELGLVELFKKYDVRMRLFHGRGGSVGRGGGPSYQAILAQPAG
                     SVAGQIRITEQGEVITAKYADPGNAQRNLETLVAATLEASILPDKKDPDAKLMQALSD
                     VSFKYYRELITHPDFIDYFLQTSPIQEIATLNLGSRPASRKTLARIQDLRAIPWVFSW
                     MQNRLMLPAWYGFGSAVETLCEDKPETLAALREHAQSNPFFQAMLSNMEQVMAKTDIT
                     LAENYAGLSESPDKAKVIFGMIKEEYQRSRKALLDLLQTEELLRDNRSLARSLALRIP
                     YLNALNGLQVAMLKRLRKEPDNPHALLMVHLTINGVAQGLRNTG"
     gene            18010..18903
                     /locus_tag="WIC03_000120"
     CDS             18010..18903
                     /locus_tag="WIC03_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HEZ3882687.1"
                     /translation="MPHPCQTVHTLTARVPKCRLKLNAARRQRYNRPQLFFPNIIMTT
                     TEHDNDDAFLLRYSRHILLDEIGIEGQQKLSAAHILVVGCGGLGAAALPYLAASGIGT
                     LTIADSDTVELHNLQRQVAFDEGDVGKPKAEALADRLKRINHNVDVRTVNEKLDGCRL
                     TGLVQTADIVLDCCDNYATRQAVNRVCVQTKTPLVSGAAVRFEGQLAVYRPDLPDSPC
                     YACLFDGGSASDGICSLFGVFSPLVGIIGSTQAAEALKILLDAGEPSHGRLAVYRALE
                     GGWQYFDLPRNPECPICGAER"
     gene            complement(19302..19526)
                     /locus_tag="WIC03_000121"
     CDS             complement(19302..19526)
                     /locus_tag="WIC03_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HEZ3882688.1"
                     /translation="MDAVQELERRIVELEIQSALQEDVIAGLNAMVAELRQTLDLQQA
                     QLRLLYQKMQDRNPDAQEPYSLRDEIPPHY"
     gene            complement(19527..20915)
                     /locus_tag="WIC03_000122"
     CDS             complement(19527..20915)
                     /locus_tag="WIC03_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HEZ3882689.1"
                     /translation="MNAVVVAVIVMLVLSLSRVHVVLSLTVGAFVGGAVAGMPLQNIA
                     DAAGQVSQAGIIPVFNKGLEGGAKIALSYAMLGAFAMAITHSGLPQQLAGAVVRKLNR
                     GGMPDSVRSGEGAVKWLLLSIILVMGIMSQNIIPIHIAFIPMIVPPLLLVFNRLKIDR
                     RLIACVITFGLVTTYMFLPYGFGAIFLNEILLGNIHSAAPQLDVKNINVMAAMAIPAL
                     GMLAGLLLVFVHYRKPRLYQSNNADTAGNADAANRPQPSAYRSLAAAAAIAVCFAIQL
                     MYEDSLVLGAMLGFAVFMMLGVINRDKANDVFGEGIKMMAMVGFIMIAAQGFAAVMNA
                     TGHIQPLVESSMAIFGNSKGMAALAMLVVGLLVTMGIGSSFSTLPIIAAIYVPLCVGL
                     GFSPLATVAIVGTAGALGDAGSPASDSTLGPTMGLNADGQHDHIRDSVIPTFIHYNIP
                     LLIAGWIAAMVL"
     gene            complement(21001..21822)
                     /gene="prmC"
                     /locus_tag="WIC03_000123"
     CDS             complement(21001..21822)
                     /gene="prmC"
                     /locus_tag="WIC03_000123"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEZ3882690.1"
                     /translation="MTFDEWLGLSKLPKIEARMLLQYVSEYTRVQLLTRGGEEMPDEV
                     RQRADRLAQRRLNGEPVAYILGVREFYGRRFTVNPSVLIPRPETEHLVEAVLARLPEN
                     GRVWDLGTGSGAVAVTVALERPDAFVRASDISTPALETARKNAADLGARVEFAHGSWF
                     DTDMPSEGKWDIIVSNPPYIENGDKHLLQGDLRFEPQIALTDFSDGLSCIRTLAQGAP
                     DRLAEGGFLLLEHGFDQGAAVRGVLAENGFSGVETLPDLAGLDRVTLGKYMKHLK"
     gene            complement(21812..22273)
                     /locus_tag="WIC03_000124"
     CDS             complement(21812..22273)
                     /locus_tag="WIC03_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3882691.1"
                     /translation="MQEQNRKSSFPIVMLLVSVALWIASLSNVAFYLGNHGSMEGLTV
                     LILGSIFASLDIRYCAVYANYVWLAAIVLLALRKKVVPVHAALWGLALVAFSVKAVYV
                     DEAGNTSDIVRYGAGFYLWYAAFAVATIGTFAGKNKERKAASAADGIKNDV"
     gene            complement(22338..23822)
                     /gene="tldD"
                     /locus_tag="WIC03_000125"
     CDS             complement(22338..23822)
                     /gene="tldD"
                     /locus_tag="WIC03_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HEZ3882692.1"
                     /translation="MPYNQAPDFLCADTMHPTYSAVQARLLEANRLSPELLAKSLCII
                     GAHHVDYADIYCQRTAYESWHLEEGIVKSGSFQINQGVGVRAVSGDKTAFAYADSLCI
                     DSINRSARAVRAIGAAGGKVSAKMPSETRGKPVCSASDPIAGLDSAAKVALLNKVEAI
                     AKAADPRIVQVMAGLTCEYDMVYLARLDGKHAADIRPMVRMNVTVIAKQGERREQGSA
                     GGGGRYDLAYFDENLVHRFVDSAVKQALTNLESRPAPAGEMTVVLGNGWPGVLLHEAV
                     GHGLEGDFNRKGTSVFSGRIGERVAAKGVTVVDQGDIAGRRGSLNIDDEGNETRRTVL
                     IEDGILVGYMQDETNARLMGTQSTGNGRRESYASVPMPRMTNTFMENGGYEPDEIIAS
                     IDKGIYAVNFGGGQVDITSGKFVFSASEAWWVEGGRLQYPVKGATIIGNGPEVLKHVS
                     MIGNDTALDSGVGVCGKEGQSVPVGVGQPTLRIDAGLTVGGSAI"
     regulatory      23897..24000
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            24067..25290
                     /gene="cytX"
                     /locus_tag="WIC03_000126"
     CDS             24067..25290
                     /gene="cytX"
                     /locus_tag="WIC03_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydroxymethylpyrimidine transporter
                     CytX"
                     /protein_id="HEZ3882693.1"
                     /translation="MSGNASSPSSSAAIGLIWFGAAVSIAEISTGTLLAPLGWQRGLA
                     ALLLGHAVGGALFFAAAYIGALTGRSSMESVRLSFGKRGSVLFSVANMLQLAGWTAVM
                     IYAGATVSSALGKVLWDGESFVWWALANGALIVLWLVFGARKTGGLKTVSMLLMLLAV
                     LWLSAEVFSTAGSTAAQVSDGMSFGTAVELSAVMPLSWLPLAADYTRHARRPFAATLT
                     ATLAYTLTGCWMYALGLAAALFTGETDVAKILLGAGLGAAGILAVVLSTVTTTFLDAY
                     SAGASANNISARFAETPVAVGVTLIGTVLAVMLPVTEYENFLLLIGSVFAPMAAVLIA
                     DFFVLKRREEIEGFDFAGLVLWLAGFILYRFLLSSGWESSIGLTAPVMSAVAIATVSV
                     RLFFKKTQSLQRNPS"
     gene            25287..26387
                     /locus_tag="WIC03_000127"
     CDS             25287..26387
                     /locus_tag="WIC03_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEZ3882694.1"
                     /translation="MTRIAILGGGLSGRLTALQLAEQGYQIALFDKGCRRGEHAAAYV
                     AAAMLAPAAEAVEATPEVVRLGRQSIPLWRGIRCRLNTHTMMQENGSLIVWHGQDKPL
                     SSEFVRHLKRGGVADDEIVRWRADDIAEREPQLGGRFSDGIYLPTEGQLDGRQILSAL
                     ADALDELNVPCHWEHECAPEDLQAQYDWLIDCRGYGAKTAWNQPPEYTSTLRGIRGEV
                     ARVYTPEITLNRPVRLLHPRYPLYIAPKENHVFVIGATQIESESQAPASVRSGLELLS
                     ALYAVHPAFGEADILEIATGLRPTLNHHNPEIRYSRKRRLIEINGLFRHGFMISPAVT
                     AAAVRLAVALFDGKDAPERDEESGLAYIRRQD"
     gene            26408..27025
                     /gene="thiE"
                     /locus_tag="WIC03_000128"
     CDS             26408..27025
                     /gene="thiE"
                     /locus_tag="WIC03_000128"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEZ3882695.1"
                     /translation="MTFPPLKSPLKFYAVVPTADWVGRMVKAGADTVQLRCKALHGDE
                     LKREIARCAAACQGSRTQLFINDHWREAIEAGAYGVHLGQEDMDTADLAAIAAAGLRL
                     GLSTHSVAELDRALSVHPSYIASGAIFPTTTKQMPTAPQGLDKLREYVKQAGGTPVVA
                     IGGIDLNNARAVLATGVSSLAAVRAVTEAANPEAVVKAFQALWDG"
BASE COUNT         5856 a         7713 c         7276 g         6420 t
ORIGIN      
        1 acaaacctag attcccgcct gcgcgggaat gacggggttt taggtttctg attttggttt
       61 tctgtttttg agggaatggc cgattttggg tttctgtttc ggttttctat tttgcaagaa
      121 tggcaaaatt tcagattgcg ggcattgtta agtatttcta ttttttacct gccgtattta
      181 tttccgcccc ttgaagtcgg cttcttcctc gacagacacg ctgttcatct gtttgatcag
      241 cttttccgac ttctcttcgt cttcgcagcg gatgactttc gcaatatcgc tttcgagctg
      301 tccgacattg ctgtgcagaa tgatgttttt gacgggcagg atgttgttgg ggttcatgga
      361 aaaacggcgc agccccatac ccaataaaac gcgggtaaac gcggtatcgc ccgccatctc
      421 gccgcatacg gatacgtctt tgtccatgcg gttggcggta cggatgacgt gttgcagcat
      481 tttcagcacg gcgggatggc cgggctggta gagatggctg acgctgtcgt cgccgcgatc
      541 gacggacaag atgtattgaa tcaggtcgtt ggtaccgacg gagatgaaat cgaccagttt
      601 caaaatactg ccgacggtca gcgcggcaga cggaatttca atcatacagc cgatgccgac
      661 tttaccgaag gcatcgccgc gttcggcaag ctggcgttgc gcggtgtcga ggtggatgag
      721 gcactggcgc acttcggata cggaggtaat catcggccac atcatgcgga cgggaccgtg
      781 taccgccgca cggaggatgg cgcgcatctg ggtgcggaac atgaccggtt cggcaaggca
      841 caggcggatg ccggtcatgc ccagcgcggg gttgaggctg ccgttgggcg tgctgttttt
      901 cccgaaccag cgcgggtttt tgtccacacc caaatcgact gtccgtatcg ttacgctttt
      961 gcctttcatt tttttgacaa tcgcgctgta cacttcgtac tgctcgtctt cagacggcat
     1021 cgtatcgcgg ttcaggtaaa gaaactcgct gcggaacagc ccgatgccgt ctgcgccgag
     1081 attgtgcagc ggtttcacgt cttcggcgga ttctatattg cccacaagct cgatgcagac
     1141 cccgtcggcg gtggcggcgg cggttttttt gagcttgttc aaatcgcgtt tgtggctgcg
     1201 gtattcgcgg gcacggcggc ggtattcgtt caacaccgac tcatccggcg cgataatcaa
     1261 cacgccgttg ataccgtcca caatgaccgt ttcgccctcg gtaatcagtt tgcgcgcgtt
     1321 gtgcagcccg acgacggacg ggatgtccaa gctcctgccc aaaatcgccg tatgcccggt
     1381 ggggccgccg gcatcggtaa cgaaggcggc aatgcgctgc tctttaaaca aaaccgtgtc
     1441 ggcgggcgaa aggtcgtttg caatcagaac ggtttcgtca aacaggttgt cggcaacttc
     1501 caactcgttg ccctgcccga tcaggttgtt gtggatgcgg cggacgactt gcagcatatc
     1561 ctgcttgcgt tcgcgcaaat aggcatcgtc catattgtcg aattgggcgg cgagtttgtc
     1621 gctctgctgc ttcaatgccc actcggcgtt gattttttgt tcccttaaaa tatcgacggg
     1681 ttcgcgcgac aaggtaacat cggtcaagag catcaggtgc agcgagatga acgcgcccaa
     1741 ctcggtcggg gcgttttcgg gaatcgcgct gcggagctgt tccaactctt tgcgcgtggc
     1801 tttgacggcg gcatcgaaac gttcggcttc ggcatcggtg tccgcctccg caacatcata
     1861 ctgcggcact tcctccgtac cgcgcgcaat caggtgggcg caaccgacgg caatgccttt
     1921 gcccgccgcc acgccgtgca gcacgatact cattattcgc cctcgccgaa gtagccgttg
     1981 attaagtcgg tcagggcgcg catcgcttcc gcctcgtccg cgccgtccgt ctccagttcg
     2041 atgaccgtac ccttggcggc ggcgagcatc atcagcccca taatgctttt gccgttgacg
     2101 cggctgtcgt ttttcgtaac ccagacttcg cttttgaatt gggacgcggt ttgggtgaac
     2161 ttgttggacg cgcgggcgtg gagtccgagt ttgttgatga tttcgatgga ttgtttgagc
     2221 atttcgattc ccgtgttatg tatatcggca gcagacgccg tttaaaatgt tttcctgccc
     2281 tgccgcttct tcagacggca tcgccgctgc gccggcacac caaatcttcg ggcgcggacg
     2341 tgatggcgaa aatgcctttt accgccgcct ccctgacgca ttcggtaaag gcggcaaggt
     2401 cttccgccgc cggcgaatat tggacggcct taaccatcat cggcgcgttc agcccggtca
     2461 aaatcgccga tttgttttcg cgcacgaggc ggcgggctgc attgcagggg gtcgcgccga
     2521 aaatatcggt cataattagc acgccgtcgt tgtcgggaaa ctcctgaagc gcggcaatgg
     2581 cgttgttgat gatgtcgtct tggtcttccg tcggttgcac gccgagtatg cggacgtttt
     2641 caggcagtcc gcccggaaaa aaatgatgtg ccagcttgcg gtaggcttcg cctatggttt
     2701 cgtgtgtgat gattaaaagc cctatcatat tatgcgtcct gttcctcgtt gtcctaccgg
     2761 cgtatgggcg cgatgccgtc tgaaaggcct tcagacggca tcgcgtcctt attttccgtc
     2821 caatgcgtaa atctcgccca gattgcgcca gcagcccgcc gcatccatgc cgtaaccgaa
     2881 aacataacgg ttcggcacat ccagtccgac ataatcggct cggataggct tgggtttgtc
     2941 aatcagcttg ttggcgaaca ccgccgcacg gcagcttgcc gcacccattt ccaaaagttt
     3001 ggcttgaatg gcggacatcg tatgcccttc gtccaaaata tcgtccagca cgacgacgtg
     3061 cctgccccgg atttgttccg catcgggcat acgcttccag ttgaacgcgc cgccctccag
     3121 cttgtcgccg taacgggaaa cgtgaacata atcaaaatct aagggaaaac gcaacagcgg
     3181 cagcaactgc cccgtaaaca ccaccgcgcc gcccatcacg ggcagcagca gcggatattt
     3241 gccgcccaaa tcgcgcgtaa tctcgtccgc cactttttgc agtgcggcac ggcattggcc
     3301 ttggtcgaac aaaagatcgg cgttttcaag catcgcctgt gtttcaaggc gtttggtttc
     3361 taaatcggtc atatgtcgga atcggtcggt aaaaggaaaa ttataaacca aagtatcgga
     3421 tgccgtctga actgtcctgc tcagcggtcg gtacgcacgc gcacaaacgt ggcgaatttc
     3481 ggcgtgcctt tctgcgtaaa gccacggtag cggtaggtaa tcagtgtgcc gattttgggc
     3541 gggttgtcgc ggtctttatc tttgaagccg ctgccgatgc ggaattcgcc gtgtcggttt
     3601 ttgcagccga ccgcgcccaa ccgtccggcg tttcgccctt tgccctcata gtgccgcgtt
     3661 accgtgcatt cgtcgtcgta ttggcttttc agcttcaata attggctgct cctgccgccg
     3721 ctgtaacggg attcgggctg gcgcagcatc acgccttcgc cgccctgcgc ttcgatttgt
     3781 tttaaaaagt ccatcgcgtg ctgccggacg cgcactttga tttgcgggat gatggtaatc
     3841 ggcgcgttcg gatgcgtttt cagccactgc gttgcgactg ccaaacgttg gtagagattg
     3901 ccctgcgcct tgggtacatc gaaaacgtgc aggcggatgc cgcgccagtc tgaagaaaca
     3961 gaacgcacgg tagcggaaat ctgctcgaac tgaccacgtc cgctatacaa ttcgccgtcc
     4021 aaaggataag gcggaaactg agcggtaaaa cctttggggg gcgtaaacgc atagccctga
     4081 cggcttatca ggtgctttcc gtcccaatac gcgcgcacgc cgtcgagttt ctcgctcatc
     4141 gcccagcctg caatctcttg acctttgtat tcctgcgcca gcatcaaatc cgccgcgcct
     4201 gctgatgcag ggatgaaaac cgccgtaaaa atcggtatga tgccgccgat tgtcttctta
     4261 atcatctgat tcccccaata tcaaaacggg cggcaaaccg ccataaaaca aacggcaaac
     4321 ccgatgccgt ctgaaaaacc gtttaggaac acgccgatga ccctacgtta cgaaatcttc
     4381 cccgttaccc ccttccgcca aaactgcacc ctgatttggg acgacgaaag cggcgaagcc
     4441 gtcctgaccg atgtcggcgg cgacgtgccg ttcctgctgc aagcgttggc aaaccgcaaa
     4501 cttacgctca cggcaatctg gctgacgcac ggccatctcg atcacacggg cggcgtggtc
     4561 gaaatgttga aaacgcataa agtccctgtc ctcgggccgc atccggacga tgaattcctg
     4621 ctccaatcgc tgccgcaaac caccgcgcaa tacggatttc ccgtctcgcc cgcctttgcg
     4681 ccgaaccgtt ggctcgaaga aggcgaaacg ctcaaagtcg gacgctacgc cttccaagtg
     4741 ctgcatattc cggggcacac gccgggacac gtggttttct attgtgccga agcggagctg
     4801 ctgattgcgg gcgacgtgtt gttttatgaa accataggca gaaccgattt tccgcgcggc
     4861 aaccacgccg acttaatcaa taatatccgc aacaaattat tcgccctccc cgaaaccgtg
     4921 caagttgtcg ccggacacgg gcgtatgact tccatcggac acgaaaaacg gcacaatccg
     4981 tttttctaac caacttccca aagtcttcag acggcgtcat ctgcactgat gccgtctgaa
     5041 acacaaaagg ctcagacaac cgccgccttg ccggtgtacc tcgccggaca aggctttggt
     5101 aagtacttca aaccaacccc aaatcagaac cggtacagtt acgccgcgct ttcggcattc
     5161 ccgcccctgc tgaaacaata tttttccgca caagtcagac tgcttcatct tctgccgcat
     5221 attccaaaga ttccgacaac gccgttgttt cattggcacg ttcggtcaaa tcgcgtgcaa
     5281 gctgcttgtt tgccttcaca cccaacagac gcagtttctc ggcgcggccg accagattcc
     5341 cgcgcccttc ggcaagttgc ttgaatgccg tctgaaagct gttttgcgcc tgatcgatgc
     5401 ctttgccgac gctttcgagc gtctgtacga agccgacaaa cttgtcgtac agcttgccac
     5461 cttcatccgc aatcgccagc gcgttctgat tttgctgttc gttgcgccaa atattcgcca
     5521 ccgtcctcaa agtcgccagc agcgtactgg ggccgaccag cataatccgt ttgtcgaaac
     5581 actcttggaa caatcccgcg tcatgctgca acgccagcag gtaggccggt tcgacaggga
     5641 taaacataaa gacgaaatcc aatgtgttta caccttccaa atcggtgtaa tccttcagcg
     5701 acaagccttt catgtgtgca cggatgctgg caacgtgtgc cgccagttcg cgtgccgccg
     5761 tatccgcatc cgccgcctgc gtgtagcgca cataagctgt cagcgagacc ttggagtcaa
     5821 tcacaatctg cttgttgtcg ggcaggttga ccaaaacgtc gggctggagg cggcgcgtgc
     5881 cgccgtcttc ctccttacgg acagatgctg cctgaaccgt atattcccgt cccttctgga
     5941 ggccggaatt ttctaaaacc gtttccagaa tcatctcgcc ccaattgccc tgaaccttat
     6001 tctgcgtacc ggtcagcgcg ttggtcaggg cctttgcctc gctgtgcagc tgcgcgttca
     6061 acccctgaag ccgtttcaat tcgttttcca acgtcagccg ctcgcgcgat tctttatcat
     6121 aggtttgctt gaccaactcg ccgaaaccgt ggatgcgttc gtttagcggg ttcaaaacct
     6181 gatggagctg ctcgcggttc tgctcggtaa aacggcggct tttttcttcc aaaatcgtgt
     6241 tggcaagatt ttgaaactga tcgctcaaac tcttgcgcgc ctcgcccagc aaggacagct
     6301 tctcttcaga agcaaggcgt tcctgttcga tttgcgttgc caaacgttcg ttttcaaccg
     6361 ccaaaccctg tgccttttcc tgcaactcgg tatgcgactg cctcaaccgc tccgcttccg
     6421 cctctttttc ctgcaaatgg gcaatctgtt tttcggctgc ggcaaaacgg ttgccgacat
     6481 cggcaaggtc gttttgcacg tcgcggacag tttggcggct ttcttccaaa tcggtttcga
     6541 ttctttggcg gatttggcgt tccaaggcat attggtcggc aaacgccttc cgttcctgtc
     6601 cgagctgcgt tgccaaacgt tcgttttcaa ccgccaaacc ctgtgccttt tcctgcaact
     6661 cgatatacga ctgcttcagc cgcgccgatt ccgcctcttt ttcctgcaaa tgggcaatct
     6721 gcttttcggc tgcggaaaaa cggttgccca aagcataatt ttcgtcctgc aaatgccgat
     6781 atttcccgtc caacaccgcc aattccgatg caatttctgc gtgtgcctgt tcgacaaaat
     6841 cacatcttgc cgccttttcc gccagttgcg cgttcaaatc ggcaaactcg ccctgaaacc
     6901 tgcccttcat cagcaaccat gtaaacaaca cgcccgacac caacgccgcc aaaggcagca
     6961 aaacagtcat cagttccatc aatcatccta atattcaaac attttcacac cggacaaaac
     7021 cgccgcttat tccgattcta cctgtttgtt cgacatagct ccaaaaaata tagcggattg
     7081 gctttaaacc tgttcgacat cgccttacca tgctgcttgc ggtttcagac cttttcctaa
     7141 ttcaatatcc atccgccgca aaccctgatt aagttcccga tgtctgacat ttttagaatg
     7201 atgccgtctg aaatgttgca gctatgttca gacggcatac ggattcaggc ttttcaaacg
     7261 gcaggcaaaa tgaaaaaagg gcaaacccta aaggatttgc ccttttgttc caaacgctta
     7321 gtgtacgtct ttccaatatt cttttttcag gaaatacgcc aaaggcagca taaccgcaaa
     7381 taggaaaatc atcacgacat agcctatacg tttgcgttgc agttgtgcag gttcgcccat
     7441 gtacacaagg taattgacca aatcgcgtac atatgcgtcg tactcttttt ggatcacttt
     7501 gccgttaggc agacggcggc tgtgcaaacc ggtagattcc caatacagct taggcttcat
     7561 ctcgccgtgt tcgtctttta ccataaccgg ctgacctttg gcatccaact caacggcttg
     7621 aacgccttgc tgttcccaca acgggtgggg cataccgact ttatcgaata cagtattgtt
     7681 ccagccgctc ggacgggtcg gatctttata gaagccgcgc atataggcgt aaaggtaatc
     7741 tgcacctttg gaacgcgcaa tcaacgtcaa atcgggcgga gcagcaccaa accattttgc
     7801 cgcatctttc gggttcatcg ccgaatgcat gacatcgccg acattatcgg tggtaaacat
     7861 caggtttttc ttgatttctt cgtcagtcaa accgatgtct ttcaaacggt tgaaacgcat
     7921 accgcttgcc gagtggcagg acaggcaata gttggtaaag atttgtgcgc cgcgctgcag
     7981 gctgacttgg tcgcgcaaat cgatatcgac tttctcatag tgtgcatgac cgcccgaagc
     8041 aaatgccgca cccatgggta ctgccaacag caaggcagca aaccaatttt tcatagcttg
     8101 tttcattttg actgccctca tcagatgttg gttgcaaaca ggtaagcacc cacggcggta
     8161 atgccgacgt aaacaaagaa catgattttt tgtttggcgg tgctcatggt tacgcgttcg
     8221 ggaaccggtt tgtttttatc cagcttggta tagaacggca tacccaggaa gaatgcgaaa
     8281 tagacgaagg acaggatgcg cgcgaccaaa gtacgcgtat cggttgccac catcgcgccc
     8341 aaaataccca aaccgataaa ggaaatgatg aacaagacca atgcggtttt gaaaatcgga
     8401 ccgcggtaac ggacggattt cacctcgccc ctatccagcc aaggcagcag ggcaatcaac
     8461 acgaccgccg cgcccatacc gattacaccc caaacctgcg ttcccaagaa cgagggaatc
     8521 gcacgcaaaa tggcgtagaa cggtgtgaaa taccataccg gcgcaatgtg cgggggtgtt
     8581 ttcagcgcat ttgccgcatc gaagttcggc gcttcgagga agtagccgcc gccttccggt
     8641 gcgaagaaca atacggcaca gaagacaatc aggaatacaa ccacacccaa gatgtcttta
     8701 acggtatagt aaggatggaa tgggataccg tcgcgcggga taccgttttc gtctttgttt
     8761 ttcttgattt caacgccgtc agggttgtta gaacccactt cgtgcagggc gatgatgtgc
     8821 gccacaacca agccgagcaa taccagaggt accgcgataa cgtgcagggc aaagaaacgg
     8881 ttcagggtaa cgtcggaaac gttgaagtcg ccgcgaatcc aagtggacaa atccggaccg
     8941 ataacaggga tggcggagaa caggttaata attacctgcg ccccccaaaa ggacatctga
     9001 ccccaaggca gcaggtagcc cataaaggct tccgccatca atgccaagaa aatcagagaa
     9061 ccgaaaatcc acaccaactc acggggtttt ttgtacgaac cgtaaatcaa accacggaac
     9121 atgtgcagat aaacaacaat gaagaagaaa gatgcgccgg tggaatgcat gtagcggata
     9181 atccaaccgc cggacacgtc gcgcatgatg tactcgactg cagtaaaggc agcaggcaga
     9241 tggtaggcgt tcaggttgcc gtcgggtttg tagttcatgg tcaggaaaat gccgctgacg
     9301 atttgaatca cgaggacaag cagggccaaa gagccgaagt agtaccaaaa attaaagttt
     9361 ttcggtgcgt agtattgagc caaatgctca ttccacattt tagacagggg gaaacgagcg
     9421 tccatccagc ctaacaatgc ttttgctttg ctattggttt ggtttgccat aattatcgtt
     9481 ccttattctt agtcttcgcc caccaagata gttgtgtcgc tcaagtattt atatggcggg
     9541 acaaccaggt tggtcggggc aggaacacct ttatatacgc ggccggccaa gtcgaatttc
     9601 gaaccgtggc aggggcagaa gaagccgcct ttccagtctg cacccaaatc ggcgggggca
     9661 atgtcgggac ggaaggtggg cgagcagccc aaatgggtgc agataccgat ggcgacaagg
     9721 atgttcggct taatcgaacg ggtctcgttt ttagcatact ccggctgctg ttccgcatcg
     9781 gaattgggat cggtaagttc gccgttcagg cctttcaggt ctttaagctg ctgatctgta
     9841 cggttgagca cccaaatcgg tttgccttgc cactcggcgg tcagcagctg acccgcttcg
     9901 attttactga catccacctc gacggcagca ccggcggcct tggctttttc cgaagggaaa
     9961 aaactggcca caaacggcgt tgccacaccc aatgctgcca ctccgcccgc gccgcaggtc
    10021 gcgagtgtca ggaaacggcg gcggccgttg ttgatttctt gattatccat tattcagtcg
    10081 tcctaatatt ttgggaatac cgagccatta aacgttgcaa ttttacccag tttgcagtga
    10141 tactcaaagc attatttaaa ataaggtaaa gttttatgat atttctcaag actcaagccg
    10201 gattgttttc gtcaaaatgg cacacttcca acccgaaaac ctctgccgcc gattctgcca
    10261 gcgcgcgtac gccgtaacgt tccgtcgcgt gatgccctgc cgaaatgaaa gccgtacccg
    10321 tttcattggc aaggtggtat tgggcttcag agatttcccc cgtcaaatac agatcgacac
    10381 cttcgtctat tgccgtctga aaaaacccct gcgccccgcc gctgcaccat gcaacccgtc
    10441 ggatttcgcg ttcgggtttg ccgatgacga caggcttacg ttgcaaaact gtttcaatat
    10501 gcgccgccaa tgcgccgagt gtcttggctt gtttcaggct gcccgagttg agcaggtttt
    10561 gttcgccgaa ccgtttttct gtcgcaaaac ccaatctgtc ggcgagttgg gcattgttgc
    10621 ccagtgtggg atgtgcatcc aggggcagat ggtagcctgc catattgatg tcgtgccgta
    10681 acagtgcggc aatccgttct tttttccaac cagtaacggt cggcaactcg tttttccaga
    10741 acataccgtg atgtaccaaa agcaaatctg ccttctgctc cacagcaaaa tcaatcgctg
    10801 ccctgcttgc cgttaccgac gtaacgattt tccctatata ttccctccct tcaacctgca
    10861 aaccgttagg ggcgtaatct ttaaacaacg ctgtctgcaa tgtttcatta caccaagtca
    10921 gaaaatccct gcgcaatacc atcttttttc ctaatcgctt taaacaagcg ggcattctaa
    10981 tcgcaaaatg tccggaattc acatttttcc gatttgcacc cgcatatgaa ttattttaat
    11041 atgcgccggt tcaatatgcc gtctgaagcc ccatggattc cattatcgaa ctgcgccacc
    11101 tcaagaccct gctggcactt gaagaaaccg gcagcgtctc ccttgccgcc aaacgggttt
    11161 tccttaccca atctgcactt tcccaccaaa tccgtatgct tgaaaaccac tacggcgcac
    11221 cgttgttcga acgaaaatcc acgcccttgc gctttacccc ggtgggcgaa aggctgctgc
    11281 gcctcgccca cgaactgata cctcaagttg ctgttgcaga acgagatttg gcgcgaatca
    11341 cggaagggga ggcgggagag ctgcggattg ccgtcgaatg ccatacctgt ttcgactggc
    11401 tgatgcccgc catgggcgaa ttccgcccga tgtggcccca agtcgaactg gatatcgtat
    11461 cgggattcca agcggatccc gtcgggctgc tgctgcaaca ccgcgccgat cttgccattg
    11521 tttccgaagc ggaaaaacaa aacggtattt ctttccaacc gctgtttgcc tacgaaatgg
    11581 tcggcatttg cgcaccggac catccgcttg ccgccaaaaa cgtttggacg gcggaagact
    11641 ttatcgggga aaccctgatt acctatcccg ttcccgatga aatgctggat ttacccaaaa
    11701 aaatcttaat tccgaaaaac atcaacccac cgcgccgaca cagcgaactg accattgcca
    11761 ttatccaact ggttgccagc cggaggggaa tcgccgccct tccctattgg acagtcatgc
    11821 cctaccttga aaaaggctat gtcgttcacc ggcagattac cgacgacgga ttgcaaagca
    11881 aactgtatgc tgccatccgc actgaagaca cagataaaag ttatctgaac aacttttgcc
    11941 aaatcatacg cgaacgcggt tttgcagatt tgcccggact gagcgaactg gaaccggtct
    12001 gaccccttat tcaaccatac ccggcagttt ttctattttt tcatgtatag tggattaaca
    12061 aaaaccagta cggcgttgcc tcgccttgcc gtactatttg tactgtctgc ggcttcgtcg
    12121 ccttgtcctg atttttgtta atccactata ctgtttttga tttttgccca atctgtaatc
    12181 tttagattgc caatgggaaa ccgtctacta caaataaaaa accctgcgat aagcagggtt
    12241 ttttgaattt ccaacattaa cgtttggaga attgttttgc acggcgtgct ttgcgcagac
    12301 ccggtttttt acgttcaact tcgcgggcat cgcgggtaac aaaaccagct tgagacaagg
    12361 cgggtttcaa cgcggcatcg aagtcgatca gggcacgggt aatgccgtgg cggattgcgc
    12421 cagactggcc ggtttcgccg ccgccaacaa cattgacttt gatgtcgaaa gattcggcgt
    12481 tttcagtcag aaccaagggt tggcgaacaa ccattcggct ggtttcgcgt gcgaagaatt
    12541 cgtcaacggg acggccgttt acgatgattt gacctgtacc tttaatcagg aatacacgag
    12601 ccactgaact tttgcggcgg cctgtgccgt agtagtattt accgttcatg tcgcgtcctt
    12661 atttcagttc caaaactttg ggttgttgcg cagcatgggc gtgttccgca cccgcataca
    12721 ctttcagttt tttaatcatg gcgtaaccca aaggaccttt gggcagcata ccttttacag
    12781 cttgttccaa agcgcggccc gggaattgct cttgcatttc gcggaaagtg cgttcgtaga
    12841 taccgcctgg gaaaccggaa tggcggaagt attttttatc ttcgaatttg gcaccggtta
    12901 cacgcagttt atccgcattg ataacaatga tgtaatcgcc ggtatcgacg tggggggtgt
    12961 attcaggttt gtgtttgcca cgcagacggc tggcgacttc ggccgcaacg cgacccaaga
    13021 ctttgtcttg ggcatcgatg acgaaccatt cgcgcttcac ctcgtggggt ttcgctgaaa
    13081 aggttttcat agtggaaatc cagatagata tagaaagttg taaattttaa agacaggatt
    13141 cgattttgtc aatcgcatta ccgcgttacg gaaggatttt ccggatttcg gcagactgca
    13201 tactgctttt tcgggcgggg cggcggccaa tatgaaaaac cgcatcgttg cgatgcggtt
    13261 ttgaatggga atccccgcga gagccgtttc ggccgaatcc gcttgaacct tgctgacaag
    13321 gcggctgcct cgggtagttt cgggtgcgtc cgcaaaagga cgctcgcgcc cactactgct
    13381 cccggcaacc ttaagcgaac ttattggttc aaaggaatat atgccttcgc ggacaccgca
    13441 gggaaaaagg ggttattcct gcgccaagcg ggatagtgct ttttggcagg cgttgtccat
    13501 atcggctatt ttacgcgcaa aatcgccgat tgccaaatcg ccgccgttca gggaggtttt
    13561 caacaggtcg tggacgacgt tgagcgcggc cataatgacg attttttcgc tgtccgcgac
    13621 gcgtccgcct tcgcggatgg cttcggcttt gccgttgagc attccgactg cctgcaacag
    13681 tgtgtctttt tcttctgccg gcgtgttgac ggtcagccgg gcgtgcatga cttcgatgtg
    13741 gacttgttcg atgttcatcc tttaatcctt attgctgcgt ttcctgccat tgggggaggc
    13801 gcgctgccag tgcgctgatt ttttccctgc tctgttcgag caggctgcgg tatcgtgtat
    13861 tttcttctgt caggctgtca attttgtttt gcaggtcttc tttgagtttg ccgacttgga
    13921 cgagcagggc ttcgctgagt tcgtcgacgg cggtttcgtg ttcgagtttt tgccgctcgt
    13981 gcgcccgttt gagttcggcg acggtttctt tgaggcggcg gttttcgctg acgagggttt
    14041 cgaatttttg taccagcgta taaacgctgc tttcgagttt ttcgatattt tgtttcataa
    14101 ccttacctgt ccgtatgccg tctgaaggct tcagacggca tctgtctgtt gtttattcaa
    14161 aacgcgcgct acgttccatc agcctttcga cgacctgctg aggggtcatt tctttgcgga
    14221 tgagttgcag cagggtttgg gtaatcggca tgtcgatttg gtacttacag gcagtattga
    14281 agacttcttc tatcgtgctg accccttcgg aaacgtgtcc gatttcgacc agcacctgat
    14341 gcagttcctt gccttctgcc aaacccaagc cgacgcggcg gttgcgcgaa agtgcgccgg
    14401 tgcaggtgag gatgaggtcg ccgataccgg caagccccat catggttttg ggctgtgcgc
    14461 ccattgcgga ggcaaggcgg gtaatttcag ctaatccgcg cgtaaccagt gcggcacggg
    14521 cgttaagccc gtactctagg ccgtcggaca atccggtggc aatcgccata acatttttta
    14581 ccgcgccgcc aaccgccacg ccgataacat cggtactgcc gtaaagcctc atgacggtcg
    14641 tgttgagctg cggtacgagt tcttcaatcc actcttggtt ttcggaggcg aggacgacgg
    14701 cgcagggcag ttgtttggcg agttcctgtg caaaactcgg gccggaaagt acgccgattt
    14761 ttttattgtc gggcaatact tctttcaata cttgaaaggt cagcagccct gtatcctgct
    14821 cgaatccttt gcaggcggcg aggacgggga ggtgtcccgc gccgtactgt ttgagcagct
    14881 ctgcgctgct tctcaatccg gcaacggagg ttacgataag gacaagtccg ctgtctttga
    14941 gcgcgtctgc caaatccgca cacacttcca aggtttcggg aaaggaaaag ccgggcagtc
    15001 cgcgtttgtt ttcacgcgct tcctgcattt ggcggacttg gtctgcgttg cgcgtccaca
    15061 gggatacgcg gttgccgtgt tgggaaaaat gcagggcgag cgccgtaccc cacgaacctg
    15121 cgccgataac ggtaattttc attggtcgtc tttcaacata tcactgccgt tcactttaaa
    15181 acaatcggtg tttctctgca agtgcggtca gggaaatgcc gtctgaaagg cgttcagacg
    15241 gcattttgcc ccgatgcggc actatcagcc tgtattgcgc aaaccttgcg ccacgccgtt
    15301 gatggtcagg tgcaccatca gaagggcgtg cggattgtcg ggttctttac gcaggcgttt
    15361 gagcatggcg acttgcaggc cgttgagggc gttcaaatat ggaatcctca aggcaagcga
    15421 acgggcgagg ctgcggttgt cgcgcaggag ctcttcggtt tgcagcaagt cgagcagtgc
    15481 tttgcggctg cgttggtatt cttccttgat catgccgaag atgacttttg ctttgtcggg
    15541 tgattcgctc aagccggcat agttttccgc caaagtgatg tcggttttcg ccatcacttg
    15601 ctccatattg gagagcatgg cttggaagaa cggattgctt tgggcatgtt cgcgcagggc
    15661 ggcgagcgtt tcgggtttgt cttcgcacaa ggtttccacc gcgctgccga aaccgtacca
    15721 agcaggcagc atgaggcggt tttgcatcca ggaaaatacc cacggaatcg cgcgcaagtc
    15781 ttgaatccgc gctaaggtct tgcggctggc aggacggctg ccgaggttga gggtggcgat
    15841 ttcctgaatc gggctggttt gcaggaagta gtcgatgaag tcggggtggg taatcagttc
    15901 gcggtagtat ttgaacgata cgtccgacaa tgcctgcatc agtttggcat cagggtcttt
    15961 tttatccggc aggatgctgg cttccaaagt cgcggcaacc aaggtttcca agttgcgttg
    16021 ggcattgccg gggtcggcgt atttggcggt aatgacttcg ccttgttcgg taatgcggat
    16081 ttgtcccgcc acgctgccgg ccggttgcgc caaaatggct tggtaagaag ggccgccgcc
    16141 gcgtccgacg ctgccgccgc gtccgtggaa caggcgcatg cggacatcgt attttttgaa
    16201 gagttcgacc aagcccaatt ccgcctgata gaggcaccat gagctggtaa cgtagccgcc
    16261 gtccttgttg gagtcggagt agcccagcat gatttcttgg atattgccgc ggctttcgag
    16321 cagggcatcg taccagtcga ggcggaacat ggtttccatg accggacagg cgttttccaa
    16381 cgcttcgatg gtttcaaaca gcggcacgat attgatgcgg ctttgcggtt tgccgttttc
    16441 taccgccaac aggccgcttt ctttcagcag caatgccaag gcgagcaggt cgctgggttg
    16501 ttcgcagttg gaaataatgc tttgcgtaac ggcatcttcg ccgaattcgt ctttgatttt
    16561 gcgcgcttca ttgaaaatcg ccagttcgtg gcgagtatgg tcgctgtatg tgatgaacgg
    16621 actgtacagt ggacgttgat ggctcaattc gcgcaacagg gcggcttgtt tttgctcttc
    16681 attcaggctg ttgtagtctt ccaagcctgc gtgttggaaa agctcggcaa ccacatcggc
    16741 gtgtttgcct gcgtgttggc gcaggtcgag cggcatcatg tggaagccga acacggacac
    16801 ggaacggatg aggtctgcca gacggccttc ggcaagcagg cggctgccgt tgtcgataag
    16861 ggaacgttgc aattttttca aatcatcgag aaattcttgt gccgaagcat aaggctcgag
    16921 aaagccgaat ttgcagccca tgcccaaacc gagcgcgcgc gctttgccca tagcgcgcgc
    16981 catgatgtaa gcaatggcgc ggcggtaggg ttcttccgtg cgggcaagtt cttcgtcagg
    17041 cgagagggct gccagtgcca ttacgtcgtc gttgactttg acgcggcgga tggagagcgg
    17101 cagttcgcgg taaagtttgt cgagttcgcc acggtaaaaa cggaacacgg catcggcgtg
    17161 gcggcggaag gcaaaacgca aggtttcggc agaaacgaac ggattgccgt cgcggtcgcc
    17221 accgatccag ccgccgattt tgaggatgtt cggaacgcag acatcgggat agaccatctg
    17281 aaagtcgtgt tccatattgc ggtagagctt gggcagggct tcgaaaaagc tcatcgggaa
    17341 gatggacacg ccgttgttga tttcgtcgtt gacgctgagt ttgtggcggc gcgtttcgct
    17401 ggtctgccac aagcccagca ggatagtgtc gatttcgcgg cgcagccgtg ccagcgcgtc
    17461 ggcattggtg cagcgttcgc gttgcggcaa cagtgcgcgg atgcggcggt tgaagcttaa
    17521 gacggtttgg cgttgcactt cggtcgggtg cgcggtcaaa acggcggtaa cggacgtatt
    17581 gtccaactgc cgctgcaccg atttgccgtc ggctttcccc gctttgagcc tgcggacggt
    17641 ttccgtcagg ctgccttccg cgccgccgcg tccggcttct tcgtggattt ggcggcggcg
    17701 ttcgtggtgc acgtcttcgg cgatattcaa aatctgggcg aacaggccgc aggccaaggt
    17761 taaatcgtgg gtttgttgtt cgtccaattg cggcaatact ttttcaatca atgccgcgct
    17821 gtcgtcggaa gtggacaaga gtttgaccgt ttcgacaacc aatggcgagg cttcttcgtg
    17881 caggaggttg aacagggatt gtttcagaaa ttccgcgtcc gccgccaaag ccgcgtcctt
    17941 tggattgttc agaatatgca gttgcatgat ttttctctca ttgccgtaaa tactgtaaat
    18001 gtacctcaaa tgccgcatcc gtgccaaacc gttcacactt taaccgcccg tgtcccgaaa
    18061 tgccgtctga agttgaacgc cgcccgacgg cagcgttaca atcgcccaca actgtttttt
    18121 ccgaacatca tcatgaccac gaccgaacac gacaacgacg atgcattcct gctgcggtac
    18181 agccgccaca tcctcttgga cgaaatcggc atcgaagggc agcagaaact ttccgccgcg
    18241 catattttgg tcgtcggctg cggcggtttg ggtgccgccg ccctgcccta tctcgccgct
    18301 tccggcatcg gcacgctgac catagccgat tccgacacgg tcgagctgca caacctgcaa
    18361 cgccaagtcg catttgacga gggcgatgtc ggcaagccca aagccgaagc cttggcagac
    18421 cgcctgaaac gcatcaacca taacgtcgat gtccgcaccg tcaacgaaaa actcgacggc
    18481 tgccgcctga ccggtttggt tcaaaccgcc gacatcgttt tagactgctg cgacaactac
    18541 gccacgcgcc aagccgtcaa ccgtgtctgc gtacaaacga aaacaccgct ggtttcagga
    18601 gcagcggtgc gctttgaagg gcaacttgcc gtgtaccgtc ccgacttgcc cgactcgccg
    18661 tgttacgcct gcctgtttga cggcggatcg gcttcagacg gcatctgttc cctcttcggc
    18721 gtgttctcgc cgctggtcgg catcatcggc agtacccaag cggcggaggc tctgaaaatc
    18781 ctgctggatg cgggcgaacc gtcgcacggc aggctggcgg tttaccgtgc cttggaaggg
    18841 ggctggcaat atttcgacct gccgcgcaac cccgaatgcc cgatttgcgg cgcggaacgg
    18901 taaaaccctg ccgccgtttc agacggtatc caaacggatg cggcggaacg gttttaaaaa
    18961 tttaaaaatt tacatttctt tgcaaaaaaa ataaacttac cttataattg cagttgtttt
    19021 agcaatgtct gtttcgcaga ctcattgagt aagacgtttt ccccgtaatg tgtttggccg
    19081 tctgtcccct ttgggttcgg acggcttttt tttggctgtg tatgaatacc cggttggttt
    19141 tatctgttta caggggaaaa ccgcttattt ccgttcgggc ggaaaacggt tccatcggat
    19201 aaaaggcatt ttgtccgact gattaaagtt acatcttagg tttgcatcgg caaaatattc
    19261 aaacacagcc ttttttaagg aaatccggat acggcggcgc atcaataatg cggcggaatc
    19321 tcgtcgcgca gggaatacgg ctcttgcgcg tcgggattcc tgtcctgcat tttttgatac
    19381 agcagcctca actgagcctg ctgcaaatcc agcgtctgcc gcaattccgc caccatcgcg
    19441 ttcaggccgg cgattacgtc ctcctgaagc gcggattgga tttccagttc gacaatacgg
    19501 cgttccaact cttgaaccgc gtccatttac agcaccatcg cggcaatcca gccggcaatc
    19561 agcagcggga tgttgtagtg gatgaaggtc gggataacgg aatcgcggat gtggtcgtgc
    19621 tgcccgtcgg cgttcagccc catcgtcggg cccagcgtgg aatcggacgc aggcgaaccg
    19681 gcatcgccca acgcccccgc cgtgccgaca atggcgacgg tggcaagcgg cgaaaaaccc
    19741 aaaccgacac acaaaggcac ataaatcgcg gcaataatcg gcaaagtgga aaaggacgaa
    19801 ccgatgccca tcgttaccaa aagccccacc accagcatcg ccaacgccgc catacctttg
    19861 ctgttgccga atatcgccat actgctttcc accagcggct gaatatgccc ggtcgcattc
    19921 atcacggcgg caaaaccctg cgcggcaatc ataatgaagc cgaccatcgc catcatcttg
    19981 atgccttcgc cgaatacgtc gtttgccttg tcgcggttga tgacgcccaa catcataaat
    20041 acggcgaaac cgagcatcgc gcccaacacc agcgagtctt catacatcaa ctggatggca
    20101 aagcatacgg caatggcggc ggcggcggcc aggctgcggt aggcggacgg ctgcggacgg
    20161 tttgccgcat cggcgttgcc cgccgtatcg gcattgttgc tttggtacag gcgcggtttg
    20221 cggtaatgga caaacaccag caggagtccg gccagcattc ccaacgcggg aatcgccatt
    20281 gccgccatta cgttgatgtt tttcacatca agctgcggcg cggcggaatg gatgttgccc
    20341 aacaggattt cattcaaaaa aatcgcgccg aagccgtaag gcaggaacat ataagtcgta
    20401 accagcccga aagtgatgac gcacgcaatc aggcggcggt cgattttcag gcggttgaac
    20461 accaaaagca gcggcggaac aatcatcggg ataaaggcga tgtggatggg gatgatgttc
    20521 tgactcataa tgcccatcac aaggatgatg gaaagcagca gccatttgac cgcgccctcg
    20581 cccgaacgca cgctgtcggg cataccgccc cggttcagct tgcggacgac cgcgccggca
    20641 agctgttgcg gcaggccgga atgggtaatc gccattgcaa acgcgccgag catcgcataa
    20701 gaaagcgcaa tcttcgcacc gccttccaaa cctttgttga acacggggat aatccccgcc
    20761 tgactgacct gtcccgccgc atcggcaatg ttttgcagcg gcatacccgc caccgcgccg
    20821 ccgacaaacg cgccgaccgt caggctcaat accacgtgca cgcgcgacag cgacagcacc
    20881 agcataacga ttacggcaac tacgactgca ttcattgttt atcgctccaa acctataaat
    20941 gtttacatat cgaaacacat cataacccaa taatggaaaa cccgccaatt ttgcaaacaa
    21001 ttatttcaaa tgcttcatat acttccccag cgtaaccctg tccaaacccg ccaagtccgg
    21061 cagggtttcc actcctgaaa aaccattctc cgccaacacg ccgcgcaccg ccgcgccctg
    21121 atcgaaaccg tgttccaaca acaaaaaacc gccttccgcc aaacggtcgg gcgcgccttg
    21181 cgccaaggtg cggatgcagc ttaggccgtc tgaaaagtcg gtcagcgcga tttgcggctc
    21241 aaaccgcaaa tcgccttgca acaaatgttt atcgccgttt tcgatatagg gcgggttgga
    21301 cacgatgatg tcccatttcc cttcagacgg catatcggtg tcgaaccacg aaccgtgtgc
    21361 aaattcgacc cgcgcgccca aatctgccgc atttttccgc gccgtttcaa gggcgggcgt
    21421 gctgatgtcg gatgcgcgca caaacgcatc ggggcgttcg agtgcgacgg ttacggcaac
    21481 cgcgccgctg cccgtcccca aatcccacac gcgcccgttt tcgggcaggc gcgccaatac
    21541 ggcttcgacc aagtgttcgg tttcggggcg cggaatcagc acgctcggat tgactgtaaa
    21601 gcgtctgcca taaaattcgc gcacacctaa aatataggca accggctcgc cgttcagacg
    21661 gcgttgcgcc agcctgtccg cccgctgtcg gacttcgtcc ggcatttctt ccccgccccg
    21721 cgtcaacaac tgcacgcgcg tatattccga aacatattgc agcagcattc ttgcttcaat
    21781 tttaggcagt tttgacaagc ccaaccattc gtcaaacgtc attttttatc ccgtctgccg
    21841 ccgatgcggc ttttctttcc ttattctttc cggcaaacgt accgatggtg gcaaccgcaa
    21901 atgcggcata ccacaaataa aatcctgcac cgtagcgcac aatatccgat gtattccctg
    21961 cttcatcgac gtatacggct ttcacactga aagccaccaa cgccaagccc caaagtgccg
    22021 catggacagg cacgaccttc ttccgcaacg ccagcaaaac aatggccgcc aaccaaacat
    22081 aattcgcata gaccgcacaa tacctgatat ccaaagaagc aaatatcgac cccaaaatca
    22141 aaacggtcaa accctccatg cttccatgat tgcccaaata aaatgcaaca ttggataaag
    22201 acgctatcca cagggcaacc gacaccagca acatcactat gggaaaactt gatttccgat
    22261 tctgttcctg catggtttta tcctaatgta aaaggccgcc tgaaaacctt tcagacggca
    22321 tcgtgccgga ttccgcgtca gattgcgctg ccgccgacgg tcagtccggc atcaatccgc
    22381 aaagtcggtt gccccacgcc gacggggacg ctctgccctt ctttgccgca cacgccgaca
    22441 ccgctgtcga gcgcagtatc gttgcctatc atggaaacgt gtttcagcac ttcggggccg
    22501 ttgccgataa tggtcgcgcc tttgacgggg tattgcagcc tgccgccctc cacccaccac
    22561 gcttcggacg cgctgaacac gaatttgccg ctggtaatgt ccacctgtcc gccgccgaag
    22621 ttgacggcgt aaatgccttt gtcgatggac gcgatgattt cgtcgggttc gtagccgccg
    22681 ttttccataa aggtattggt catgcgcggc atagggacgg aggcgtagct ttcgcggcgg
    22741 ccgttgccgg tcgattgcgt gcccatcagg cgggcattgg tttcgtcctg catataaccg
    22801 accaagatgc cgtcttcaat caatacggtg cggcgggttt cgttgccttc gtcgtcgatg
    22861 ttgagcgaac cgcgccggcc ggcaatatcg ccttgatcca caacggtaac gcctttggcg
    22921 gcgacgcgct cgccgattcg gccggaaaag acgctggttc ccttgcggtt gaaatcgcct
    22981 tccaaaccgt gtccgaccgc ttcgtgcagc aacacgcccg gccagccgtt gcccaaaacg
    23041 acggtcattt cgccggcagg cgcaggacgg gattcaagat tggtcagggc ttgtttgacg
    23101 gcggaatcga caaaccgatg caccaagttt tcatcgaaat aagccaagtc gtagcgtccg
    23161 ccgccgcccg cgctgccctg ttcgcggcgt tcgccctgtt tggcgataac ggtaacgttc
    23221 atgcgcacca tcgggcggat atcggcagcg tgtttaccgt ccagacgggc gaggtaaacc
    23281 atgtcgtatt cgcaggtcaa accggccatc acttgcacga tgcgcggatc ggcggctttg
    23341 gcgattgctt ccactttgtt caacagcgcg actttggcgg cggaatcgag gccggcaatg
    23401 gggtcggacg cggaacaaac cggcttgccg cgcgtttcag acggcatttt ggcggacacc
    23461 ttgccgcctg ccgccccaat cgcgcggacg gcgcgggcgg aacggtttat cgaatcgatg
    23521 cacaggctgt cggcgtaggc aaaggcggtt ttatcgcccg aaacggcgcg cacgcccacg
    23581 ccctgattga tttggaagct gcccgatttg acgatgccct cttccaaatg ccagctttca
    23641 taagcggtgc gctggcagta gatgtcggcg taatcgacgt ggtgcgcgcc gatgatgcac
    23701 aggcttttgg cgagcagttc gggggaaagg cggttggctt cgagcagccg cgcctgtacg
    23761 gcggaatagg tcggatgcat agtgtcggcg cataaaaaat caggggcttg attatacggc
    23821 atttgttatg gaaatgcgcc gtgccgccga aaatgtaaga attttgccaa cgcacgttgc
    23881 tttgtgtaaa cttaaagctt tcttgtcgga gtgccgccgc cgggcggctg agattgcgaa
    23941 agcagaatcc gtagaacctg tcggggtaat gcctgcgtag gaaacaaacc gtcaaatgcc
    24001 ttatcaggct tccgttccct tttccgcact tccccgccca ccttccttct ttttaaagga
    24061 cgtcatatgt cgggcaatgc ctcctctcct tcatcttccg ccgccatcgg gctgatttgg
    24121 ttcggcgcgg cggtctcgat tgccgaaatc agcacgggta cgctgcttgc gcctttgggc
    24181 tggcaacgcg gtctggcggc tctacttttg ggtcatgccg tcggcggcgc gctgtttttt
    24241 gcggcggcgt atatcggcgc actgaccgga cgcagctcga tggaaagcgt gcgcctgtcg
    24301 ttcggcaaac gcggttcagt gctgttttcc gtggcgaata tgctgcaact ggccggctgg
    24361 acggcggtga tgatttacgc cggcgcaacg gtcagctccg ctttgggcaa agtgttgtgg
    24421 gacggcgaat cttttgtctg gtgggcattg gcaaacggcg cgctgattgt gctgtggctg
    24481 gttttcggcg cacgcaaaac aggcgggctg aaaaccgttt cgatgctgct gatgctgttg
    24541 gcggttctgt ggctgagtgc cgaagtcttt tccacggcag gcagcaccgc cgcacaggtt
    24601 tcagacggca tgagcttcgg aacggcagtc gagctgtccg ccgtgatgcc gctttcctgg
    24661 ctgccgcttg ccgccgacta cacgcgccac gcgcgccgcc cgtttgcggc aaccctgacg
    24721 gcaacgctcg cctacacgct gaccggctgc tggatgtatg ccttgggttt ggcagcggcg
    24781 ttgttcaccg gagaaaccga cgtggcaaaa atcctgctgg gcgcaggttt gggtgcggca
    24841 ggcattttgg cggtcgtcct ctccaccgtt accacaacgt ttctcgatgc ctattccgcc
    24901 ggcgcgagtg cgaacaatat ttccgcgcgt tttgcggaaa cacccgtcgc tgtcggcgtt
    24961 accctgatcg gcacggtact tgccgtcatg ctgcccgtta ccgaatatga aaacttcctg
    25021 ctgcttatcg gctcggtatt tgcgccgatg gcggcggttt tgattgccga ctttttcgtc
    25081 ttgaaacggc gtgaggagat tgaaggcttt gactttgccg gactggttct gtggcttgcg
    25141 ggcttcatcc tctaccgctt cctgctctcg tccggctggg aaagcagcat cggtctgacc
    25201 gcccccgtaa tgtctgccgt tgccattgcc accgtatcgg tacgcctttt ctttaaaaaa
    25261 acccaatctt tacaaaggaa cccgtcatga cccgtatcgc catcctcggc ggcggcctct
    25321 cgggaaggct gaccgcgttg cagcttgcag aacaaggtta tcagattgca cttttcgata
    25381 aaggctgccg ccggggcgaa cacgccgccg cctatgttgc cgccgccatg ctcgcgcctg
    25441 cggcggaagc ggtcgaagcc acgcccgaag tggtcaggct gggcaggcag agcatcccgc
    25501 tttggcgcgg catccgatgc cgtctgaaca cgcacacgat gatgcaggaa aacggcagcc
    25561 tgattgtgtg gcacgggcag gacaagccat tatccagcga gttcgtccgc catctcaaac
    25621 gcggcggcgt agcggatgac gaaatcgtcc gttggcgcgc cgacgacatc gccgaacgcg
    25681 aaccgcaact cggcggacgt ttttcagacg gcatctacct gccgaccgaa ggccagctcg
    25741 acgggcggca aatattgtct gcacttgccg acgctttgga cgaactgaac gtcccttgcc
    25801 attgggaaca cgaatgtgcc cccgaagacc tgcaagccca atacgactgg ctgatcgact
    25861 gccgcggcta cggcgcaaaa accgcttgga accaacctcc cgaatacacc agcaccttgc
    25921 gtggcatacg cggcgaagtg gcgcgggttt acacacccga aatcacgctc aaccgccccg
    25981 tgcgcctgct gcacccgcgt tatccgctct acatcgcccc gaaagaaaac cacgtcttcg
    26041 tcatcggcgc gacccaaatc gaaagcgaaa gccaagcccc cgccagcgtg cgttcagggt
    26101 tggaactctt gtccgcactc tatgccgtcc accccgcctt cggcgaagcc gacatcctcg
    26161 aaatcgccac cggcctgcgc cccacgctca accaccacaa ccccgaaatc cgctacagcc
    26221 gcaaacgccg cctcatcgaa atcaacggcc ttttccgcca cggtttcatg atctcccccg
    26281 ccgtaaccgc cgccgccgtc agattggcag tggcactgtt tgacggaaaa gacgcgcccg
    26341 aacgtgatga agaaagcggt ttggcgtata tccgaagaca agattaaagc cgtctgaaag
    26401 gaaccccatg accttcccgc cactcaaatc cccgctcaaa ttctacgccg tcgtccccac
    26461 cgccgattgg gtggggcgca tggtcaaagc aggtgccgac acggtgcaac tgcgctgcaa
    26521 ggccctgcac ggcgatgaat tgaaacgcga aatcgcccgc tgcgccgcag cctgtcaggg
    26581 cagccgtacg cagcttttca tcaacgacca ctggcgcgaa gcaatcgaag cgggcgcgta
    26641 cggcgtgcat ctcggacaag aagacatgga caccgccgac cttgccgcca tcgccgccgc
    26701 cggtttgcgc ttgggtttga gtacgcactc cgttgccgaa ctcgaccgcg ccctgtccgt
    26761 acaccctagc tacatcgcca gcggcgcgat tttcccgacc acgaccaaac aaatgcccac
    26821 cgccccgcaa ggcttggaca aactgcgcga atacgtcaaa caagcaggcg gcacgcccgt
    26881 cgtcgccatc ggcggtatcg acttgaacaa cgcccgagcc gtactcgcca ccggcgtttc
    26941 ctcactcgcc gccgtccgcg ccgtaaccga agcggcaaat cccgaagcgg tggttaaagc
    27001 gtttcaggct ttgtgggatg gataaaaccg aaagaagaaa attcaattgc cgtgtcggca
    27061 aaacttagcc cgttatcgca aacatactta actttaaatg tggcatatca tcaaattccg
    27121 tcattcccgc gtaagcggga atccgcctta aaacttgaga aaccatcatt tgaaaaacag
    27181 tttccgaatt tcaaaaatgg attcccgccc gtgcgggaat gacggcaacc ggtcagttgc
    27241 gtatcaaaaa ataaagtaat tcggc
//
LOCUS       DAWHSK010000005        26099 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.005, whole genome shotgun sequence.
ACCESSION   DAWHSK010000005 DAWHSK010000000
VERSION     DAWHSK010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 26099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26099
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.005"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            20..809
                     /locus_tag="WIC03_000129"
                     /pseudo
     CDS             20..809
                     /locus_tag="WIC03_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245498.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            850..1380
                     /locus_tag="WIC03_000130"
     CDS             850..1380
                     /locus_tag="WIC03_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEZ3882696.1"
                     /translation="MNAIRTFQNRTPEIHESCMIDEACVVIGEVSLAEDVSVWPCAVL
                     RGDVNSITVGARSNIQDGSVLHVSHKTAAKPEGSPLVIGEDVTVGHKVMLHGCRIGNR
                     VLVGMGTTVLDDAVIEDEVMIGAGSLVPPRKRLEGGYLYVGSPVRQVRVLTDEEKAFL
                     KYSAAHYVKLSKQYGM"
     gene            complement(1397..1528)
                     /locus_tag="WIC03_000131"
     CDS             complement(1397..1528)
                     /locus_tag="WIC03_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor domain protein"
                     /protein_id="HEZ3882697.1"
                     /translation="MPSEPEKRLSDGIFACNSNAAGQNQGASDTVIGHPVRMNLGLT"
     gene            complement(1537..3132)
                     /locus_tag="WIC03_000132"
     CDS             complement(1537..3132)
                     /locus_tag="WIC03_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEZ3882698.1"
                     /translation="MFQEILDQVRRRRTFAIISHPDAGKTTLTEKLLLFSGAIQSAGT
                     VKGKKTGKFATSDWMDIEKQRGISVASSVMQFDYKDHTVNLLDTPGHQDFSEDTYRVL
                     TAVDSALMVIDAAKGVEAQTIKLLNVCRLRNTPIVTFMNKYDREVRDSLELLDEVENI
                     LQIRCAPVTWPIGMGKNFKGVYHILNDEIYLFEAGGERLPHEFDIIKGIDNPELEQRF
                     PLEIQQLRDEIELVQAASNEFNLDEFLAGELTPVFFGSAINNFGIQEILNSLIEWAPA
                     PKPRDATVRMVEPDEPKFSGFIFKIQANMDPKHRDRIAFLRVCSGKFERGMKMKHLRI
                     NREIAASSVVTFMSHDRELVEEAYAGDIIGIPNHGNIQIGDSFSEGEQLAFTGIPFFA
                     PELFRSVRIKNPLKIKQLQKGLQQLGEEGAVQVFKPMSGADLILGAVGVLQFEVVTSR
                     LANEYGVEAVFDSASIWSARWVSCEDKKKLAEFEKANAGNLAIDAGGNLAYLAPNRVN
                     LGLTQERWPDIVFHETREHSVKL"
     gene            complement(3239..3634)
                     /gene="hisI"
                     /locus_tag="WIC03_000133"
     CDS             complement(3239..3634)
                     /gene="hisI"
                     /locus_tag="WIC03_000133"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEZ3882699.1"
                     /translation="MDKNLLEAVKFDEKGLVCAIAQDAETKRVLMVAWMNAEALQKTV
                     ETGFAHYYSRSRQKQWMKGEESGHTQKVRELRLDCDGDAIVMLIAQNGGIACHTGRES
                     CFYKVWRGSAWETADAVLKDEKEIYGSTH"
     gene            complement(3665..4432)
                     /gene="hisF"
                     /locus_tag="WIC03_000134"
     CDS             complement(3665..4432)
                     /gene="hisF"
                     /locus_tag="WIC03_000134"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEZ3882700.1"
                     /translation="MALAKRIIPCLDVKDGRVVKGVNFIGLRDAGDPVEAAKRYNGEG
                     ADELTFLDITASSDNRDTILHIIEEVAGQVFIPLTVGGGVRTVADIRRLLNAGADKVS
                     INTAAVTRPDLIDEAAGFFGSQAIVAAVDAKAVNSENTRWEIFTHGGRNPTGLDAVEW
                     AIEMQKRGAGEILLTGMDRDGTKQGFNLPLTRAVAEAVDIPVIASGGVGNVRHLIEGI
                     TEGKADAVLAAGIFHFGEIAIREAKRAMREAGIEVRL"
     gene            complement(4445..5182)
                     /gene="hisA"
                     /locus_tag="WIC03_000135"
     CDS             complement(4445..5182)
                     /gene="hisA"
                     /locus_tag="WIC03_000135"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEZ3882701.1"
                     /translation="MLLIPAIDLKEGRCVRLKQGLMEEATVFSDSPAETALHWFKQGA
                     RRLHLVDLNGAFAGVPQNLPAIKDILAAVAKDIPVQLGGGIRDLKTIGQYLDLGLNDV
                     IIGTAAVKNPDFVREACKAFPGRIIVGLDAKDGMAAIDGWATVTGHHVIDLAKRFEDD
                     GVNSIIYTDIGRDGMMSGVNIDATVKLAQAVRIPVIASGGLTGLDDIRALCAAEKHGV
                     AGAITGRAIYEGSIDFAQAQQLADSLD"
     gene            complement(5215..5853)
                     /gene="hisH"
                     /locus_tag="WIC03_000136"
     CDS             complement(5215..5853)
                     /gene="hisH"
                     /locus_tag="WIC03_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEZ3882702.1"
                     /translation="MQIAIIDYGMGNLHSVLKSVRTAGQLAGKNTEIFLSGDPDRVSR
                     ADKVIFPGQGAMPDCMAALTRGGLDEAVKDALKNKPFFGICVGAQLLFDHSEEGNTDG
                     LGWFGGKVRRFERDLRDPQGCRLKVPHMGWNTVRQTQNHPLFKDIPQDTRFYFVHSYY
                     FAPENPETILGESDYPSPFACIVGKDNVFATQFHTEKSHDAGLTMLKNFLNW"
     gene            complement(5984..7486)
                     /gene="pta"
                     /locus_tag="WIC03_000137"
     CDS             complement(5984..7486)
                     /gene="pta"
                     /locus_tag="WIC03_000137"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEZ3882703.1"
                     /translation="MAKVLIVPVSAGLDASAAAQAFAKALDTQIFQAVDATAETLLAQ
                     GKSDDWFDALVGKVAALDAANLVIEGIAPDADKIYLAGKNVELALSLDAAAVFAVRSD
                     NADADELANRLNLAKQFFAAAPGVLEGFVVDGAAASVAEAAAEKTGLTFFGSSDALKD
                     VSVLAGREAKRLSPAQFRYNLIDFARQADKRIVLPEGAEPRTVQAAAICHEKGIARCV
                     LLAKREEVEAVAKERGISLPDSLEIIDPASLVEQYVEPMCELRKSKGLTPEDARKQLQ
                     DTVVLGTMMMAQNDVDGLVSGAVHTTANTIRPALQLIKTAPGASLVSSVFFMLLPNQV
                     LVFGDCAVNPNPTAQQLADIAIQSADSAKAFGIDPKVAMISYSTVNSGSGPDVDTVIE
                     ATKLAREKRPDLAIDGPLQYDAATVPGVGKSKAPGSPVAGQATVLVFPDLNTGNCTYK
                     AVQRSANVLSVGPLLQGLRKPVNDLSRGALVEDIVFTIALTAVQAKQMEG"
     gene            complement(7671..8729)
                     /gene="fbpC"
                     /locus_tag="WIC03_000138"
     CDS             complement(7671..8729)
                     /gene="fbpC"
                     /locus_tag="WIC03_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein FbpC"
                     /protein_id="HEZ3882704.1"
                     /translation="MTAALHIGHLSKSFQNTPVLNDISLSLDPGEILFIVGASGCGKT
                     TLLRCLAGFEQPDFGEISLSGRTIFSKNTNLPVRERRLGYVVQEGVLFPHLTVYRNTA
                     YGLGNGKGKTAQERQRIEAMLELTGISELAGRYPHELSGGQQQRVALARALAPDPELI
                     LLDEPFSALDEQLRRQIREDMIAALRANGKSAVFVSHDREEALQYADRIAVMKQGRIL
                     QTASPHELYRQPADLDAALFIGEGIVFPAALNADGTADCGLGRLPVQSGAPAGTRGTL
                     LIRPEQFSLHPHSAPTASIHAVVLKTTPKARHTEISLRVGQTVLTLNLPSAPTLSDGI
                     SAVLHLDGPALFFPGNTL"
     gene            complement(8750..10267)
                     /gene="fbpB"
                     /locus_tag="WIC03_000139"
     CDS             complement(8750..10267)
                     /gene="fbpB"
                     /locus_tag="WIC03_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease FbpB"
                     /protein_id="HEZ3882705.1"
                     /translation="MSPKKIPIWLTGLILLIALPLTLPFLYVAMRSWQVGINRAVELL
                     FRPRMWDLLSNTLTMMAGVTLISIVLGIACALLFQRYRFFGKTFFQTAITLPLCIPAF
                     VSCFTWISLTFRVEGFWGTVMIMSLSSFPLAYLPVEAALKRISLSYEEVSLSLGKSRL
                     QTFFSAILPQLKPAIGSSVLLIALHMLVEFGAVSILNYPTFTTAIFQEYEMSYNNNTA
                     ALLSAVLMAVCGIVVFGESIFRGKAKIYHSGKGVARPYPVKTLKLPGQIGAIVFLSSL
                     LTLGIIIPFGVLIHWMMVGTSGTFALVSVFDAFIRSLSVSALGAILTILCALPLVWAS
                     VRYRNFLTVWIDRLPFLLHAVPGLVIALSLVYFSINYTPAVYQTFIVVILAYFMLYLP
                     MAQTTLRTSLEQLPKGMEQVGATLGRGHFFIFRTLVLPSILPGITAAFALVFLKLMKE
                     LTATLLLTTDDVHTLSTAVWEYTSDAQYAAATPYALMLVLFSGIPVFLLKKYAFK"
     gene            complement(10335..11330)
                     /gene="fbpA"
                     /locus_tag="WIC03_000140"
     CDS             complement(10335..11330)
                     /gene="fbpA"
                     /locus_tag="WIC03_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     FbpA"
                     /protein_id="HEZ3882706.1"
                     /translation="MKTSIRYALLAAALTAATPALADITVYNGQHKEAAQAVADAFTR
                     ATGIKVKLNSAKGDQLAGQIKEEGSRSPADVFYSEQIPALATLSAANLLEPLPASTIN
                     ETRGKGVPVAAKKDWVALSGRSRVVVYDTRKLSEKDLEKSVLNYATPKWKNRIGYAPT
                     SGAFLEQVVAIVKLKGEAAALKWLKGLKEYGKPYAKNSVALQAVENGEIDAALINNYY
                     WHAFAREKGVQNVHTRLNFVRHRDPGALITYSGAAVLKSSQNKDEAKKFVAFLASKEG
                     QRALTAVRAEYPLNPHVVSTFNLEPIAKLEAPQVSATTVSEKEHATRLLEQAGMK"
     gene            complement(11581..12051)
                     /locus_tag="WIC03_000141"
     CDS             complement(11581..12051)
                     /locus_tag="WIC03_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4375 domain-containing protein"
                     /protein_id="HEZ3882707.1"
                     /translation="MTDTDTQADRFEQMMWQAVDKLFEQHDGKLESMDGREQELVLIW
                     RTEADIGNGGILQFVCNWGFPAAEKTCSVLKKIGAIHSAMLIHRAADALRKEIRHLQS
                     EGKNLKEMWDITKHLDNKTASLPNSLDEQYWQDPDKLYLPGWQYYSGNPVQTLF"
     gene            complement(12108..13484)
                     /gene="argH"
                     /locus_tag="WIC03_000142"
     CDS             complement(12108..13484)
                     /gene="argH"
                     /locus_tag="WIC03_000142"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEZ3882708.1"
                     /translation="MHDKTWSGRFNEPVSELVKQYTASIGFDRRLAEWDIQGSLAHAQ
                     MLKETGVLDEGDLADIRRGMAEILEEIRSGKIEWSSDLEDVHMNIERRLTDKIGDAGK
                     RLHTGRSRNDQVATDIRLWLRDQITVIRSLIQSLQTALLDLAEQNAETVMPGFTHLQV
                     AQPVSFGHHMLAYVEMLGRDNERMADCRRRVNRMPLGAAALAGTTYPIRREITAKLLG
                     FEQICQNSLDAVSDRDFAIEFTAAASLVMVHLSRLSEELILWMSPRFGFIDIADRFCT
                     GSSIMPQKKNPDVPELVRGKSGRVIGHLIGLITLMKSQPLAYNKDNQEDKEPLFDTAD
                     TLIDTLRIYADMMRGVTVKPDNMRTAVMQGFATATDLADYLVKKGMPFRDAHEVVAQA
                     VRHADQAGVDLSKLPLEVLQGFSDLIADDVYGVLTPEGSLNARNHLGGTAPEQVRFQV
                     KRWREMLA"
     gene            complement(13503..14372)
                     /gene="galU"
                     /locus_tag="WIC03_000143"
     CDS             complement(13503..14372)
                     /gene="galU"
                     /locus_tag="WIC03_000143"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEZ3882709.1"
                     /translation="MKPIRKAVFPVAGMGTRFLPATKASPKEMLPIVDKPLIQYAVEE
                     AVEAGCTEMVFVTGRNKRSIEDHFDKAYELETELEMRHKDKLLEHVRNILPPNITCLY
                     IRQAEALGLGHAVLCARAAIGDEPFAVILADDLIDAPKGALKQMVEVYERSGNSILGV
                     ETVEPSQTGSYGIVETEHLKQFQRITGIVEKPKPEDAPSNLAVVGRYILTPRIFDLLT
                     GLPRGAGNEIQLTDGIAKLLDHEFVLAHPFEGTRYDCGSKLGYLEATVAYGLKHPETG
                     EPFRRLLEKYRTE"
     gene            complement(14400..14999)
                     /gene="rdgB"
                     /locus_tag="WIC03_000144"
     CDS             complement(14400..14999)
                     /gene="rdgB"
                     /locus_tag="WIC03_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEZ3882710.1"
                     /translation="MSEKPEKIVLASGNAGKLEEFGNLFKPYSITVLPQSTFGIPECP
                     EPYSTFIENALAKARHAAKYSGLPALADDSGICAAALNGAPGIHSARYAGSNPKSDTA
                     NNLKLAAELAGKADKSCCYVCVLVFVRHKDDPRPIIAEGVWHGQWNGVPAGENGFGYD
                     PYFYLPGHGKTAAELDSEVKNRESHRAQALAELLRKLAL"
     gene            complement(14992..15225)
                     /locus_tag="WIC03_000145"
     CDS             complement(14992..15225)
                     /locus_tag="WIC03_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3882711.1"
                     /translation="MNRRKIYLLSVALFTLAFMLLVLLGAYLLTVGSKAFAVASFLFA
                     FGALFGQIGSLALYLRHKSLRAAQSAAKENRYV"
     gene            complement(15333..15866)
                     /locus_tag="WIC03_000146"
     CDS             complement(15333..15866)
                     /locus_tag="WIC03_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEZ3882712.1"
                     /translation="MADFNQILTPGDVDGGIINVVNEIPAGSNHKIEWNRKLAAFQLD
                     RVEPAIFAKPTNYGFIPQTLDEDGDELDVLLVTEQPLATGVFLEARVIGVMKFVDDGE
                     VDDKIVCVPADDRNNGNAYKTLSDLPQQLIKQIEFHFNHYKDLKKAGTTKVESWGDAE
                     EAKKVIKESIERWNKQA"
     gene            complement(15968..16426)
                     /gene="nudB"
                     /locus_tag="WIC03_000147"
     CDS             complement(15968..16426)
                     /gene="nudB"
                     /locus_tag="WIC03_000147"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HEZ3882713.1"
                     /translation="MPMAKPLKYPVSALVVLYSGDGGILLIERTHPKGFWQSVTGSLE
                     PGETVAQTARREVWEETGILLADGQLQDWHDSTVYEIYHHWRHRYPKGVFENREHLFS
                     AEIPRDTPIALQPEEHVSYGWFGLEEAAEKVFSPSNRRAILELGRFLGKR"
     gene            16637..16713
                     /locus_tag="WIC03_000148"
     tRNA            16637..16713
                     /locus_tag="WIC03_000148"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16671..16673,aa:Pro,seq:ggg)
     gene            16871..18781
                     /locus_tag="WIC03_000149"
     CDS             16871..18781
                     /locus_tag="WIC03_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223961.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
                     /protein_id="HEZ3882714.1"
                     /translation="MGISRKISLILSILAVCLPMHAHASDLANDSFIRQVLDRQHFEP
                     DGKYHLFGSRGELAERSGHIGLGNIQSHQLGNLMIQQAAIKGNIGYIVRFSDHGHEVH
                     SPFDNHASHSDSDEAGSPVDGFSLYRIHWDGYEHHPADGYDGPQGGGYPAPKGARDIY
                     SYDIKGVAQNIRLNLTDNRSTGQRLVDRFHNTGSMLTQGVGDGFKRATRYSPELDRSG
                     NAAEAFNGTADIVKNIIGAAGEIVGAGDAVQGISEGSNIAVMHGLGLLSTENKMARIN
                     DLADMAQLKDYAAAAIRDWAVQNPNAAQGIEAVSNIFMAAIPIKGIGAVRGKYGLGGI
                     TAHPIKRSQMGAIALPKGKSAVSDNFADAAYAKYPSPYHSRNIRSNLEQRYGKENITS
                     STVPPSNGKNVKLADQRHPKTGVPFDGKGFPNFEKHVKYDTKLDIQELSGGGIPKAKP
                     VFDAKPRWEVDRKLNKLTTREQVEKNVQEIRNGNINSNFSQHAQLEREIKKLRTSEGK
                     ANSASAALLTLDLKTAQAANQVVESLRSTGNLPSNYITKSQAISMGWRPGKALGNTIK
                     NGQLGGDIFANSTNILPAKQGRIWYEADIGLINTMSRSNQAGTRLLYSNDGLLYITTD
                     HYISAIQIGTWK"
     gene            18793..19080
                     /locus_tag="WIC03_000150"
     CDS             18793..19080
                     /locus_tag="WIC03_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HEZ3882715.1"
                     /translation="MQLEIIGSKIYTEQDFHNQISKIFSIQDYYGNNLDALWDLLSTN
                     VERPITLVWKDAMFSKNQLENIFIEIVNVLERVKKQDEDYGFEEKFNYILE"
     gene            19576..20832
                     /locus_tag="WIC03_000151"
     CDS             19576..20832
                     /locus_tag="WIC03_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EndoU domain-containing protein"
                     /protein_id="HEZ3882716.1"
                     /translation="MKSKSGVIASLTGAAVGGTPVDAQTGGAVGQNAVENNLYLTSEA
                     LKKDKQTARKIYSVIKEQVKHECSSTGRITECRQNIGRIIEFTQDKRFDSRFKDLKKE
                     SLYYLNKHPDLVASYLKAEYEKLDREDKSILHRYISPGAEIVSGSLGVVLSGVAGGGS
                     CAETFGLGCAAALVGVTSSYDHVITGTKNFGKKASEQRPTIAVQALKQLGLSEQAAEY
                     VQFSIDLFSVGKSGGGIPKAKPVFDAKPRWEVDRKLNKLTTREQVEKNVQETRRRSQS
                     SQFKAHAQREWENKTGLDFNHFIGGDINKKGTVTGGHSLTRGDVRVIQQTSAPDKHGV
                     YQATVEIKKPDGSWEVKTKKGGKVMTKHTMFPKDWDEARIRAEVTSAWESRIMLKDNK
                     WQGTSKSGIKIEGFTEPNRTAYPIYE"
     gene            20863..21282
                     /locus_tag="WIC03_000152"
     CDS             20863..21282
                     /locus_tag="WIC03_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3882717.1"
                     /translation="MKRIKCFCDKFPSGDTFRMCIILDDYDNRVDYYVGIYDYITSTL
                     MSDIYYRSTIDEHFKIIELIENNPNEIYDDGGGQQFCLEFHHDKVIFYHNEFDEEDGY
                     PVLSCSLHTFKTALIAWNAFLQLPKSIHSVVETVIEE"
     gene            21319..22293
                     /gene="mafB"
                     /locus_tag="WIC03_000153"
                     /pseudo
     CDS             21319..22293
                     /gene="mafB"
                     /locus_tag="WIC03_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258126.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 2"
     gene            22304..22765
                     /locus_tag="WIC03_000154"
     CDS             22304..22765
                     /locus_tag="WIC03_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3882718.1"
                     /translation="MNLETAENIQCEFEYLALDGYFPMHFASHGQGNKDWQFAVEFIY
                     RLLICQLATLEPINFETKNDILDFCHNLAKQSPFNNDNEVWYQGEIVLTKKGIDLIKE
                     YIPEAFEEWNGKKFELNIPFIKTLKNIFVNYEVAWDENNPLFPIISLNLGT"
     gene            22791..23039
                     /locus_tag="WIC03_000155"
     CDS             22791..23039
                     /locus_tag="WIC03_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EndoU domain-containing protein"
                     /protein_id="HEZ3882719.1"
                     /translation="MEIKKPDGSWEMKKGRGGKPMTNHSMFPKDWDEARIRAEVTSAW
                     EKRKELEGQKWRGTSKSGVEIEGFTEPNRTAYPLYNSK"
     gene            23066..23485
                     /locus_tag="WIC03_000156"
     CDS             23066..23485
                     /locus_tag="WIC03_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3882720.1"
                     /translation="MYKIKCFYDQFSSGELFNYCQFLDNSSNQKINTRGTVYQYIIYV
                     LTGDLYLQKDIDENLEFIHQAENNPNEVYSGGGQGFCWDISAEKVVFYHNEFDEEDGW
                     PDLSCSLHTFKTALIAWNAFLQLPKSIHSVVETVIEE"
     gene            23563..23646
                     /locus_tag="WIC03_000157"
                     /pseudo
     CDS             23563..23646
                     /locus_tag="WIC03_000157"
                     /inference="COORDINATES: protein motif:HMM:NF026862.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            23643..23885
                     /locus_tag="WIC03_000158"
     CDS             23643..23885
                     /locus_tag="WIC03_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein 31"
                     /protein_id="HEZ3882721.1"
                     /translation="MTNLKLDFYSEVIIKDSCPNDLLENGETIKGKKGVVLGISEEDG
                     IIYGYTILLFDIKYCIYIDKKYIIPTGKKFSRDDFY"
     gene            23963..24208
                     /locus_tag="WIC03_000159"
     CDS             23963..24208
                     /locus_tag="WIC03_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEZ3882722.1"
                     /translation="MKDSTGQMRLATKDLAEAIKRGEVRSSAFTTKQLKAIEKGESKI
                     PDYTWHHHQDTGRMQLINEDLHHDTGHIGWRAMSKGK"
     gene            24211..24600
                     /locus_tag="WIC03_000160"
     CDS             24211..24600
                     /locus_tag="WIC03_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3882723.1"
                     /translation="MWKIIKEDSNDLGFAIKCLFSQSIDLNEFKLWIEQVIRDMPIED
                     IPFYIFDLADFDGGIADIDNIVGFVSSYSLPKSKKNALTGIAFLRGIDVYDPPVSKEK
                     ALKALEKYPEIYQKFQHFFPFVELPPL"
     gene            24701..25090
                     /locus_tag="WIC03_000161"
     CDS             24701..25090
                     /locus_tag="WIC03_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3882724.1"
                     /translation="MWKIIKEDSDDLGFAIKCLFSQSIDLNEFKLWIEQVIRDMPIED
                     IPFYIFDLADFDVGIGDIGNIVGFAPSSSLPKSKKNALTGIAFLRGIDVYDPPVSKEK
                     ALKALEKYPEIYQKFQHFFPFVELPPL"
     gene            25191..25580
                     /locus_tag="WIC03_000162"
     CDS             25191..25580
                     /locus_tag="WIC03_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3882725.1"
                     /translation="MWKITKEQGQDLSFATYCLLSAAINLQEFKLWLEKVVLDMPIDN
                     IHFYIFDLIDLKEGVGDIYNILDFVPNSDLSKDQDDALTGIAFLRGIDVYDPPVSKEK
                     ALKALKKHPEIYQRFQHFFPFVELPPL"
     gene            25703..>26099
                     /gene="mafA"
                     /locus_tag="WIC03_000163"
     CDS             25703..>26099
                     /gene="mafA"
                     /locus_tag="WIC03_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HEZ3882726.1"
                     /translation="MKTLLLLIPLVLTACGTLTGIPAHGGGKRFAVEQELVAASSRAA
                     VKEMDLSALKGRKAALYVSVMGDQGSGNISGGRYSIDALIRGGYHNNPESATQYSYPA
                     YDTTATTKSDALSSVTTSTSLLNAPAAALT"
BASE COUNT         6483 a         5822 c         7210 g         6584 t
ORIGIN      
        1 cagataccga acaccaaaca tcaaatatat atatcaggat aaccaaggat tatcggaggc
       61 gagaaatacc ggaataaaaa attcaaacgg aaaatatata gcatttattg attcggatga
      121 ttttattaat tgtcaggtct tgctggattt tcttggtaaa gatgatagtg atatgccgga
      181 tgtggttttt ttaaatgcgg ttaaatatga ttaagggaag tgtttcatat tttggcgaag
      241 attatcagcc tgaaaaaata ctcaatcaat ccaaagtcga agttttgaaa ggattatgcc
      301 gatttagaaa atttccgggt tcggcgtgga ataagattat aaaaagagaa ttgattatta
      361 gagaaaaact gttttttgaa aggggaattt attctgaaga tatcgaatgg tcaatgaggt
      421 tatttaatgc ggcaacaact ttttcttatt tggacggttg ttattactat tatcggcagg
      481 gaagaaaaga ttctattacg ggaactgttt cggaaaaaag tataaagtca ttattatata
      541 ttttggagaa aaatgcggaa atggaattta atagggatat atcgagttat ctttattctt
      601 ttctttccta cgaatatctc gttttgcttt ttatcatgac gagtaaaaat atagagtgtg
      661 atgctgatat aaaaagaagg gcgtatcatt taaggtttat gctgttaaag tctaataagt
      721 tgatatataa gctgatattc ccgataatca cattattcgg ggttgatatt acaggcagga
      781 ttttaaaagc aatcaggggg aatatttaat aaatccttta acactataca tcttaccgaa
      841 ggaggaaaaa tgaacgcaat ccgaactttc caaaaccgca cgcccgaaat ccacgaaagc
      901 tgtatgatag acgaagcgtg cgtcgtcatc ggcgaagtgt cgcttgccga agacgtttct
      961 gtgtggccgt gcgccgtgtt gcgcggcgat gtgaacagca tcaccgtcgg cgcgcgcagc
     1021 aatatacagg acggcagcgt cctgcacgtt tcccacaaaa ccgccgccaa acccgaaggg
     1081 tcgccgctgg ttatcggcga agacgttacc gtggggcaca aagtgatgct gcacggctgc
     1141 cgtatcggca accgcgtcct ggtcggcatg gggacgacgg ttctggacga tgccgtgatt
     1201 gaggacgaag tgatgatcgg cgcgggcagc ctcgtgcctc cgcgcaaacg cttggagggc
     1261 ggctatcttt atgtcggttc gccggtcaga caagtgcgcg tgctgaccga tgaggaaaaa
     1321 gcctttttga aatattccgc cgcgcattat gtgaagctgt cgaaacagta cgggatgtga
     1381 aatcacatcg gcgttcctac gtcagcccca aattcatgcg gacgggatgc ccgataacgg
     1441 tatccgatgc gccttgattt tgaccggctg cgtttgagtt gcaggcaaaa atgccgtctg
     1501 aaagcctttt ttcgggttca gacggcattt tatcgcctaa agtttgaccg aatgttcgcg
     1561 cgtttcgtgg aacacgatgt ctggccaacg ttcttgcgtc aaccccaaat tcacgcggtt
     1621 gggggcgagg taggcgaggt tgccgcccgc gtcgatggcg aggttgcccg cgttggcttt
     1681 ttcaaattca gccagctttt tcttgtcttc gcacgatacc cagcgcgccg accagatgga
     1741 tgcgctgtcg aacacggctt ctacgccgta ttcgttggcg aggcgcgagg ttacgacttc
     1801 aaactgcaac acgccgaccg cacccaaaat caaatccgcg ccgctcatcg gtttgaacac
     1861 ctgtaccgcg ccttcttcgc caagctgttg caaacctttt tgcagttgct tgattttcag
     1921 cgggtttttg atgcggacgc tgcggaacag ttcgggcgcg aagaatggga tgccggtgaa
     1981 cgccagttgt tcgccttcgg agaagctgtc gccgatttgg atgttgccgt ggttcgggat
     2041 gccgatgatg tcgcctgcgt aggcttcttc caccagctcg cggtcgtgcg acatgaatgt
     2101 aaccacgctg gaggcggcga tttcgcggtt gatgcgcagg tgtttcatct tcatgccgcg
     2161 ctcgaattta ccggagcaga cgcgcaagaa ggcgatgcgg tcgcggtgtt tcggatccat
     2221 attggcttgg attttgaaga taaatccgga gaatttcggc tcgtccggct cgaccatgcg
     2281 cacggtcgcg tcgcgcggtt tcggtgcagg cgcccattca atcaatgaat tgaggatttc
     2341 ctgaataccg aagttgttaa tcgccgaacc gaagaacacg ggcgtgagtt cgccggcgag
     2401 aaattcgtcg agattgaact cgttggaagc cgcctgtacc aattcgattt cgtcgcgcaa
     2461 ctgctgtatt tctaacggaa agcgttgttc caattcggga ttatcgatgc ctttgatgat
     2521 gtcgaactcg tgcggcaagc gttcgccgcc cgcttcaaag agatagattt cgtcgttcag
     2581 gatgtggtac acgcctttga agtttttgcc catgccgatt ggccaggtga cgggcgcgca
     2641 gcggatttgc aggatgtttt ccacttcgtc cagcaattcc agggaatcgc gcacttcgcg
     2701 gtcgtatttg ttcatgaacg taacaatcgg cgtattgcgc aggcggcaga cgttcaagag
     2761 tttgatggtt tgcgcttcca cgccttttgc cgcgtcgatg accatcaagg cgctgtccac
     2821 ggcggttaaa acgcggtagg tgtcttcgga gaagtcttgg tgtcccggcg tgtccaaaag
     2881 gttgacggtg tggtctttgt agtcgaactg catcacgctt gacgccacgg aaatgccgcg
     2941 ctgcttctcg atgtccatcc agtcggaagt ggcgaatttg ccggttttct tgccttttac
     3001 cgtacccgcg ctttgaatcg cacctgaaaa cagcaagagt ttttcagtca gcgtggtttt
     3061 acctgcgtca gggtgggaga tgatggcaaa cgtgcggcgg cggcgcactt ggtcgaggat
     3121 ttcttggaac atggttttct ttgcaaaaag gttcaggccg cttttcagac ggcccggaca
     3181 gtgtttgaga cggcgaaatt gtacaaaaaa atgcctgata attcaatgtt ggaggcggtc
     3241 agtgcgtgct gccgtaaatc tctttttcgt ctttcaggac ggcatcggcg gtttcccacg
     3301 cgctgccacg ccagactttg taaaagcagc tttctcgccc ggtgtggcag gcgatgccgc
     3361 cgttttgggc aatgagcatc acaatggcgt cgccgtcgca gtcgaggcgc agttcgcgga
     3421 ctttttgcgt gtgtcccgac tcttcgccct tcatccattg tttttggcgc gaacggctgt
     3481 aatagtgggc aaagccggtt tcgacggttt tttgcagggc ttcggcgttc atccacgcca
     3541 ccattaaaac acgtttggtt tcggcatctt gggcgatggc gcaaaccaaa cctttttcgt
     3601 caaatttgac ggcttcaagc aggtttttat ccatatttcc tttcagacgg catagtcgga
     3661 gcggtcagag gcgcacttcg atgccggctt cgcgcatagc gcgtttggct tcgcggatgg
     3721 cgatttcccc gaaatggaaa atgccggcgg caagtacggc atcggctttg ccttcggtta
     3781 taccttcaat caggtgccgg acattgccga cccctccgga ggcgatgacg gggatgtcga
     3841 cggcttcggc aacggcgcgg gtcagcggca ggttgaaacc ctgtttcgta ccgtccctgt
     3901 ccatcccggt gagcaggatt tcgcccgcgc cgcgtttttg catttcgatc gcccattcca
     3961 ccgcatccaa accggtcgga ttgcgcccgc cgtgggtaaa gatttcccag cgtgtgtttt
     4021 cggagttgac ggctttggca tcgacggcgg cgacgatggc ttgcgaaccg aaaaatccgg
     4081 cggcttcgtc aatcaggtcg ggacgggtaa cggcggcggt gttgatgctg actttgtccg
     4141 cgccggcatt gagcaggcgg cggatgtcgg caacggtgcg tacgccgccg ccgacggtca
     4201 gggggatgaa gacttgtccg gcaacctctt cgatgatgtg caggatggtg tcgcggttgt
     4261 cggatgaggc ggtgatgtcg aggaaggtca attcgtccgc gccttcgccg ttgtagcgtt
     4321 tggcggcttc gacggggtcg cccgcgtcgc gcaaaccgat gaagttcacg cctttgacga
     4381 cgcgcccgtc ttttacgtcg agacagggga tgatgcgttt tgccagtgcc ataatcggat
     4441 gcctttagtc gagggaatct gccagttgct gcgcttgggc aaaatcgatg ctgccctcgt
     4501 aaatcgcgcg gccggtaatc gcgcctgcta cgccatgttt ttcggcggca cacagggcgc
     4561 ggatgtcgtc caagccggtc agtccgccgg aggcgatgac gggaatgcgg acggcttggg
     4621 cgagtttgac cgtcgcgtcg atgttcacgc cgctcatcat accgtcgcgc ccgatgtcgg
     4681 tgtagatgat gctgttgacg ccgtcgtctt caaagcgttt tgccaaatca attacatgat
     4741 gcccggttac ggttgcccag ccgtcgatgg cggccatacc gtctttggca tccagcccga
     4801 caataatcct gccggggaag gctttgcacg cctcgcgcac gaagtcgggg tttttgaccg
     4861 ccgccgtgcc gataatcacg tcgtttaagc ccaaatccaa atattgtccg atggttttca
     4921 aatcgcgtat gccgccgccg agctgtacgg ggatgtcttt ggcgacagcg gcaaggatgt
     4981 ctttgatggc gggcaggttt tgcggaacgc cggcaaacgc gccgttcaaa tctaccagat
     5041 gcaggcggcg cgcgccttgt ttgaaccagt gcagcgcggt ttcggcgggc gaatcggaaa
     5101 agacggtcgc ctcttccatc agcccttgtt tcaggcggac gcagcgtcct tctttcaaat
     5161 cgatggcggg tatcagcagc ataatttttc tccttgtgcg gggccgtgtc cggcttacca
     5221 gtttaaaaag tttttcaaca tcgtcagccc ggcatcgtgg cttttttcgg tgtgaaattg
     5281 cgtggcgaat acgttgtctt tgccgacgat gcaggcaaac ggggacgggt agtcgctttc
     5341 gcccaatatg gtttcgggat tttcgggggc gaaatagtag ctgtggacga agtaaaaacg
     5401 cgtgtcttgg ggaatatctt taaacagcgg gtggttttgg gtttggcgca cggtgttcca
     5461 gcccatatgc gggactttca gacggcatcc ctgcgggtcg cggaggtcgc gctcaaagcg
     5521 tctgactttg ccgccgaacc agcccaagcc gtcggtgttt ccttcttcac tgtggtcgaa
     5581 taaaagttgc gcgccgacgc agattccgaa aaagggtttg ttttttaagg catctttgac
     5641 tgcctcgtcc aggccgccgc gcgtcagtgc cgccatacag tcgggcatcg cgccctgccc
     5701 gggaaaaatg actttgtcgg cgcgggacac gcggtcgggg tcgccgctta aaaagatttc
     5761 ggtatttttt ccggcaagct gccccgccgt ccggacggat ttcaatacgg aatgcaggtt
     5821 gcccataccg taatcgataa tggcgatttg catggcttcc tcctcttttt ttgcaatatg
     5881 gctgcgattt taacaaacaa atgtgccgtg ctgataaaaa tgccgtctga aaacgggagt
     5941 ctgtcttcag acggcatagg gtttaaaccc ggaaagccgt ttgtcagcct tccatttgtt
     6001 ttgcctgaac ggcagtcagg gcgatggtga acacgatgtc ttccaccagc gcgccgcgcg
     6061 acaggtcgtt gaccggtttg cgcaggcctt gcagcagcgg gccgacgctt aagacgttgg
     6121 cactgcgttg gacggcttta taggtgcagt tgccggtgtt caggtcgggg aagaccaaaa
     6181 cggttgcctg tcctgccacc gggctgcccg gagctttgga tttgcccacg cccggcacgg
     6241 ttgccgcatc atattgcagc gggccgtcga tggcgaggtc ggggcgtttt tcccgggcaa
     6301 gtttggttgc ttcgatgacg gtatcgacat cggggccgct gccggagttg acggtggagt
     6361 aggaaatcat cgccactttc gggtcgatgc cgaaggcttt tgcggaatcg gcagactgga
     6421 tggcgatgtc ggcaagctgt tgcgcggtcg ggttcggatt aaccgcgcag tcgccgaaga
     6481 cgaggacttg gttgggcagc agcataaaga atacgctgga cacgaggctt gcgcccggtg
     6541 cggttttaat cagttgcaaa gcggggcgga tggtgttggc ggtggtgtga accgcaccgg
     6601 ataccaaacc gtccacatca ttttgcgcca tcatcatcgt accgagtacc acggtgtctt
     6661 gcagttgctt gcgcgcgtct tcgggtgtca ggcctttgga tttgcgcagt tcgcacatcg
     6721 gctcgacgta ttgttcgacc aatgaggcgg gatcgatgat ttccaaagag tcgggcaggc
     6781 tgatgccgcg ttctttggca acggcttcga cttcttcgcg tttggcaagc aggacgcagc
     6841 gggcaatgcc tttttcgtgg cagatggcgg cggcttggac ggtgcggggt tctgcgccct
     6901 caggcaggac gatgcgtttg tcggcttggc gggcgaagtc gatcaggttg tagcggaatt
     6961 gcgccggcga caggcgtttt gcttcgcggc ctgccaatac ggatacgtct ttcagcgcgt
     7021 cgctcgaacc gaagaaggtc aggccggttt tttcggctgc cgcttcggca acggaggctg
     7081 ccgcgccgtc cacgacaaaa ccttccaata cgcccggcgc ggcggcgaag aactgtttgg
     7141 caaggttcag ccgatttgcc agttcgtcgg catcggcgtt gtcggaacgg acggcgaaga
     7201 cggctgccgc gtcaagggac aatgccagtt cgacgttttt gcctgcgagg tagattttgt
     7261 cggcatcggg cgcgatgcct tcgatgacga ggttggcggc atcgagtgcg gcaactttgc
     7321 cgaccagtgc gtcgaaccag tcgtcgcttt tgccttgcgc gagcagggtt tcggcggttg
     7381 cgtcaacggc ttggaaaatt tgtgtgtcca gtgcttttgc aaaggcttgt gcggcggcgg
     7441 aggcgtccag tccggcagat acgggtacga tgagtacttt tgccatgata tatcctttcg
     7501 tatgctgcgg tgtgcggcat atgtggttgg aaggggcggc atataggcag aaacggctgc
     7561 ctgcgtgccg tgcgtgccgt gtttggcttg aggcgcgcag gttgaatata gcaaacaaat
     7621 tctgtttcca acaagataaa tatccgcagg cttgtggatg ctgccgcctt tcagagggta
     7681 tttccgggga agaacagggc gggaccgtcc aaatggagga cggcggaaat gccgtctgac
     7741 agggtggggg cggaagggag gttgagcgtg aggacggttt gtccgacccg gagactgatt
     7801 tcggtatgcc gcgctttggg cgtggttttg agaaccacgg cgtgaatgga ggcggtgggt
     7861 gcggaatggg ggtgaaggct gaactgttcc ggacggatga gcagtgtgcc gcgtgtgcct
     7921 gcgggtgcgc cgctttgaac gggcaggcgg cccaatccgc aatcggcggt gccgtcggcg
     7981 ttgagcgcgg cggggaacac gatgccttcg ccgataaaca gggcggcatc aaggtcggca
     8041 ggttgtcggt acaattcgtg agggcttgca gtttggagga tgcgcccctg tttcatcacg
     8101 gcaatccggt cggcgtattg cagggcttct tcgcggtcgt ggctgacgaa aacggcagat
     8161 ttgccgttgg cgcgcagggc ggcaatcatg tcttcgcgaa tctggcggcg caactgttcg
     8221 tccagcgcgc tgaagggttc gtccaacaaa atcagttcgg gatcgggtgc aagggcgcgg
     8281 gcgagggcga cgcgctgttg ctgtccgccc gaaagttcgt gcggatagcg tccggcaagt
     8341 tcggaaatgc cggtcaattc caacatagct tcgatgcgct gccgttcttg cgccgtcttg
     8401 cctttcccgt tgcccagccc gtaggcggtg ttgcggtaaa cggtcaggtg ggggaacagc
     8461 acaccttcct gtacgacata acccaaacgg cgttcgcgga cggggaggtt ggtatttttc
     8521 gagaagatgg ttctgccgga aagcgaaatt tcgccaaaat cgggttgttc aaaaccggca
     8581 aggcagcgta aaagggtggt tttgccgcag ccggacgcgc cgacgataaa gaggatttcg
     8641 cccgggtcga ggctgagcga aatgtcgttt aaaactgggg tgttttgaaa acttttggac
     8701 aggtgtccga tgtgcagggc ggcggtcatg gcggtacttc ctcaagctgt tatttgaagg
     8761 cgtatttctt cagcaggaat acggggatgc cggaaaataa taccagcatc agcgcgtaag
     8821 gggtggcggc ggcgtattgt gcgtccgatg tgtattccca aacggcggtg gagagtgtgt
     8881 ggacatcgtc ggtggtcagc agcagggtgg cggtcagctc tttcatcagt ttgaggaaga
     8941 cgagtgcgaa tgcggcggta atgccgggca ggatggacgg cagtaccaac gtcctgaaaa
     9001 taaagaagtg tccgcgcccc aatgttgcgc cgacctgttc catccctttg gggagttgtt
     9061 ccaaggaagt cctcagggtg gtttgcgcca tcggcaggta aagcatgaaa taggcaagga
     9121 tgacgacgat aaaggtttgg taaacggcag gggtgtagtt gatgctgaaa taaaccaagg
     9181 atagggcgat aaccaaaccg gggacggcgt gcagtaaaaa cggcagcctg tctatccaaa
     9241 cggttaaaaa attgcgatag cgaaccgatg cccaaacaag gggcaaggca cataatatag
     9301 tcaaaatcgc acctaaagcc gatacgctta aggaacggat aaaggcatca aatacggata
     9361 cgagcgcgaa tgtgccggaa gtgccgacca tcatccaatg tatcaatacg ccaaagggga
     9421 taataatgcc caaagtcaac aagctgctta aaaaaacaat cgcgccgatc tgaccgggca
     9481 gtttgagggt tttgacggga taaggacggg caacgccttt gccgctgtgg taaatcttgg
     9541 ctttgccgcg aaatatgctt tctccaaata cgacgatgcc gcacaccgcc attaaaacag
     9601 cggaaagcag ggcggcggta ttgttgttgt aggacatttc gtattcttgg aaaatggcgg
     9661 tggtaaaagt ggggtagttc aaaatagata ccgcgccaaa ttcgaccagc atatgcaggg
     9721 caatcagtaa cacgctgctg ccgatggcgg gtttgagctg ggggaggatg gcggaaaaaa
     9781 aggtttgcag gcggcttttg cccaaggaca ggctgacttc ttcgtaagac aggctgatgc
     9841 gtttgagtgc cgcctcgacg ggcaggtagg cgagcgggaa cgaggacagg ctcataatca
     9901 tcactgtccc ccaaaagcct tcgacacgga aggtcaggct gatccaggtg aaacagctga
     9961 caaatgcggg gatgcacaaa ggcagggtga ttgccgtctg aaaaaaggtt ttgccgaaga
    10021 agcggtaacg ttggaacaaa agggcgcagg caatgcccaa aacaatggaa atcagggtaa
    10081 cgcccgccat catcgtcaag gtgttggaga gcaaatccca catacgcggg cggaacaaca
    10141 gttcgacggc gcggttgatg ccgacctgcc acgaacgcat agcgacatat aaaaaaggca
    10201 gggtaagcgg tagggcaatc agtaggatga ggccggtaag ccaaatgggt atttttttag
    10261 gagacatagt gttttttatc ggcaaaacgg gcggacagta taaatgtcca cccgtttgac
    10321 aatccgaaaa cggcttattt cataccggct tgctcaagca gccgggtggc gtgttctttt
    10381 tcggaaacag tggtggcgga cacttggggt gcttccaact tggcgatggg ttccaaattg
    10441 aaggtggata ccacgtgcgg attcaaagga tactcggcac ggacggcggt cagggcgcgc
    10501 tgtccttcct tgctggcgag gaaggcgacg aattttttcg cctcatcctt gttttgggag
    10561 gattttaaca cggctgcgcc ggaataggta atgagtgcgc cgggatctct gtggcggacg
    10621 aaattcaggc gggtgtggac attttgtacg cctttttcac gcgcgaaagc gtgccagtag
    10681 tagttgttga tgagggcggc atcgatttcg ccgttttcaa ccgcttgaag ggcgacggag
    10741 tttttagcgt aaggcttgcc gtattctttc agacctttga gccatttcaa tgcggccgct
    10801 tcgcctttca gtttgacgat ggcgacaacc tgttccaaga acgcgccgga agtgggggcg
    10861 taaccgatgc ggtttttcca tttcggcgtg gcgtaattca ggacggattt ttccaaatct
    10921 ttttcagaca gtttgcgggt gtcgtaaacg acgacgcgcg aacgtccgct cagtgccacc
    10981 cagtcttttt tggcggcaac cggcacgccc ttgccgcgtg tttcgttgat ggtggaggcg
    11041 ggcaggggct ctaggaggtt ggctgcggaa agggtggcga gtgccgggat ttgttcggaa
    11101 tagaatacgt cggcggggct tcggctgcct tcttctttga tttgaccggc aagctggtcg
    11161 cctttggcgc tgttgagttt gactttgatg ccggtagccc gggtaaaggc atctgcaacg
    11221 gcttgtgctg cttctttgtg ttggccgttg tacacggtaa tgtctgccag cgcgggggtt
    11281 gcggcggtca gggctgcggc aagcagtgcg tatcggatag atgttttcat atcgattttc
    11341 tcctaatgaa tgagagtgta caccttgtta aaacataacg gtgtgtagtg tattccttct
    11401 tctttataaa tgcaaataat tattttttaa atttgttatt atccgatccg gttattgttt
    11461 gttctgactt gtattttttc cgtgcatcgc gcccgtaagg cggaagcggc gggcaatgcg
    11521 tggcggaatg tgggtaaagg cggcattttg atttgtcgga atgcttgaga acccctctct
    11581 ttaaaacagc gtctgaacag ggttgccgga atagtattgc catcccggca gatacagttt
    11641 gtcggggtct tgccaatatt gttcatccag actgttcggc agtgaggcgg ttttgttgtc
    11701 gagatgtttt gttatatccc acatttcttt caggttttta ccttccgatt ggaggtggcg
    11761 gatttctttt ctcaatgcgt ctgccgcccg atggatcagc attgcgctgt gtatcgcgcc
    11821 gatttttttc agaacggaac aagtcttttc ggcggcggga aaaccccaat tgcagacaaa
    11881 ttgcagtatg ccgccgttac cgatatcggc ttctgtccgc caaatcaaaa ccagctcctg
    11941 ttcccgcccg tccatgcttt cgagtttgcc gtcatgctgt tcaaaaagtt tgtccaccgc
    12001 ctgccacatc atctgttcga agcggtcggc ttgggtatcc gtatcggtca tcgtgttctt
    12061 gccttttaaa aatgccgtct gaacatttct tcagacggca tcgggggtta agccaacatt
    12121 tcccgccagc gtttgacttg gaaacggact tgttcaggcg cggtaccgcc caagtggttg
    12181 cgcgcgttta agctgccttc gggtgtcagc acgccgtaaa cgtcgtcggc aatcaaatcg
    12241 ctgaaacctt gtaagacttc gagcggcagt ttgctcaaat cgacacccgc ttggtcggcg
    12301 tggcgcacgg cttgggcgac gacttcgtgg gcatcgcgga aaggcatgcc tttttttacc
    12361 aagtaatctg ccaaatcggt ggcggtagcg aagccctgca tcacggcggt gcgcatattg
    12421 tcgggtttga cggttacgcc gcgcatcata tcggcgtaaa tccgcaacgt gtcgataagc
    12481 gtgtcggcgg tgtcgaacaa gggttctttg tcttcctgat tgtctttgtt gtacgccaag
    12541 ggctgcgatt tcatcagggt aatcaagccg ataaggtgtc cgatgactcg gccggatttg
    12601 ccgcgcacga gttcgggcac gtcggggttt ttcttctgcg gcatgatgga cgaacctgtg
    12661 cagaaacggt cggcgatgtc gataaagccg aaacgcggac tcatccacaa aatcaattct
    12721 tcagacaggc ggctcaggtg aaccataacc agcgaggcgg cggcggtgaa ctcgatggca
    12781 aaatcgcggt cggatacggc atcgagcgag ttttggcaga tttgttcaaa gcccaacagt
    12841 ttggcggtaa tttcgcgccg aatcggataa gtcgtaccgg caagggcggc tgcgccgagc
    12901 ggcatacggt tgacgcggcg gcggcagtcc gccatccgtt cgttatcgcg tccgagcatt
    12961 tcgacgtagg cgagcatatg gtgtccgaag ctgacgggct gggcgacttg caggtgggta
    13021 aagcctggca tgacggtttc ggcgttttgt tccgccaaat ccagcaatgc cgtctgaagg
    13081 ctttgaatca ggcttcggat gacggtaatc tggtcgcgca gccaaaggcg gatgtcggtg
    13141 gcgacttggt cgttgcggct gcggccggtg tgcaggcgtt tgcccgcgtc gccgattttg
    13201 tcggtcaggc ggcgttcgat gttcatgtgg acatcttcca aatcggacga ccattcgatt
    13261 ttgccgctgc ggatttcttc gaggatttcc gccatgcccc gacggatgtc cgccaaatcg
    13321 ccttcgtcca acacgccggt ttctttcaac atttgcgcgt gtgccagcga gccttgaatg
    13381 tcccattcgg caagccgtcg gtcgaaaccg atggaggcgg tgtattgttt gacgagttcg
    13441 gaaacgggtt cgttgaaacg tccggaccag gttttgtcgt gcataaggat tccttgatgg
    13501 ggttattcgg tacggtattt ttccaaaagg cggcggaagg gttcgccggt ttcggggtgt
    13561 ttcaggccgt aggcgacggt ggcttccaga tagcccagtt tgctgccgca gtcgtagcgc
    13621 gtgccttcaa aggggtgcgc caggacaaat tcgtgatcga gcagcttggc gatgccgtct
    13681 gtaagctgga tttcgttgcc cgcgccgcgc ggcagtccgg tcagtaagtc gaaaatgcgc
    13741 ggggtaagga tgtagcgtcc gacgacggca aggttggagg gcgcgtcttc gggcttgggt
    13801 ttttcgacaa tgccggtaat gcgttggaac tgtttgagat gttcggtttc gacgatgccg
    13861 tatgagccgg tttgcgacgg ttcgacagtt tctacgccca aaatgctgtt gccgctgcgt
    13921 tcgtacactt cgaccatttg tttgagcgcg cctttggggg catcaatcag gtcgtcggca
    13981 aggataacgg caaagggttc gtctccgatg gcggcgcggg cgcacaagac ggcgtgtccc
    14041 aagcccagtg cttccgcctg acggatgtag aggcaggtaa tgttcggcgg caggatgttg
    14101 cggacgtgtt ccaacaattt gtctttatgg cgcatttcca actcggtttc gagttcgtat
    14161 gccttgtcga aatggtcttc gatgctgcgt ttgttgcgtc cggtaacaaa caccatttcc
    14221 gtgcagccgg cttccacggc ttcttctacg gcgtattgga tcagcggctt gtcgacgatg
    14281 ggcagcattt ctttcgggct tgccttggtg gcgggcagga agcgtgttcc catccctgcg
    14341 acggggaaaa cggctttccg tatgggtttc attctttttc ctttgtattg ttttgatgtt
    14401 taaagggcga gtttgcgtaa gagttcggca agtgcctgcg cgcggtggct ttcgcggttt
    14461 ttgacctccg aatccaattc ggcggcggtt ttgccgtgtc cgggcagata aaaatacggg
    14521 tcgtaaccga aaccgttttc accggcggga acgccgttcc attgtccgtg ccatacgcct
    14581 tcggcaataa tcggacgcgg gtcgtcttta tggcggacaa aaaccaatac gcagacgtag
    14641 caacagcttt tgtcggcttt gccggcaagt tcggcggcaa gtttcaggtt gttggcggta
    14701 tcggatttgg gattgctgcc ggcgtatcgg gcggaatgta tgcccggtgc gccgtttagg
    14761 gcggcggcac agatgccgct gtcgtcggca agtgcgggca gcccgctgta tttggcggca
    14821 tggcgtgctt ttgccagcgc attttcgata aaggtggaat agggttcggg gcattcgggt
    14881 atgccgaatg tggattgcgg caatacggtg atgctgtaag gtttgaataa gttgccgaat
    14941 tcttcgagct tgccggcatt gccgcttgcc aaaacgattt tttccggttt ttcagacata
    15001 gcggttttcc tttgcggcgg attgggcggc gcgtagggat ttgtgccgca ggtagagggc
    15061 gaggctgccg atttgtccga acagtgcgcc gaatgcgaaa aggaaggacg cgacggcgaa
    15121 ggctttgctg ccgacggtca gcagataagc acccaagagg acgagcagca taaatgccag
    15181 tgtgaagagg gcaacagaca acagatagat ttttcggcgg ttcatggcgt tcggtcggaa
    15241 acggtatgtt cggattatat ccgattggga cggtattccc tagagcttgg aaaaatgccg
    15301 tctgaagcgg cgttcagacg gcatggcggg cgttatgcct gtttgttcca acgttcgatg
    15361 gattctttga tgactttttt cgcttcttcc gcatcgcccc acgattcgac tttggtcgta
    15421 cctgcttttt tcaggtcttt gtaatggttg aagtggaact cgatttgttt gatgagctgt
    15481 tgcggcaaat cggacaaagt tttgtaggcg ttgccgttgt tgcggtcgtc ggcaggaacg
    15541 cagacgattt tgtcgtctac ttcgccgtcg tcaacgaatt tcatcacgcc gataacgcgc
    15601 gcttccaaga atacgccggt tgccaaaggt tgttcggtaa cgagcagcac gtccaattcg
    15661 tcgccgtctt cgtccaaagt ttggggaatg aagccgtagt tggtcggttt ggcgaagatg
    15721 gcgggttcga cgcggtcgag ttggaatgcg gccagtttgc ggttccactc gattttgtgg
    15781 ttgctgccgg cggggatttc gttgacgacg ttgatgatgc cgccgtccac atcgccgggg
    15841 gtcaggattt ggttgaagtc tgccatttgg tttcctttgt ttgggaaagt ttgaagtttg
    15901 aaagtatagc acaaacgtcc ggctgaaaat gcgcccgatg ccgtctgaaa gggtgtacgg
    15961 gcgcgtgtta ccgtttgccc aaaaacctgc ccagttccag aatcgcgcgc ctgttggacg
    16021 gggaaaacac tttttccgcc gcttcttcca agccgaacca gccgtaggag acgtgttctt
    16081 cgggttgcag ggcgatgggc gtatcacgcg ggatttcggc agagaagagg tgttcgcggt
    16141 tttcaaacac gccttttgga tagcggtgcc gccagtggtg gtagatttcg taaaccgtgc
    16201 tgtcgtgcca gtcttgaagc tgcccgtccg ccagcaggat gccggtttct tcccaaactt
    16261 cgcgccttgc cgtttgtgcg acggtttcgc ccggttcgag gctgccggtt accgactgcc
    16321 aaaatccttt cggatgcgtg cgttcgatga gcaggatgcc gccgtccccg ctataaagga
    16381 cgaccagtgc ggaaacgggg tatttgagcg gttttgccat cggcatcttt cggcgggctg
    16441 cggtaatgaa ggggcggatt atagcaaacg ccgcacgtta tggcgtttat ccttttccgt
    16501 atcctttttt ctgcacggat gggacgcgcc ggtgtttgcc ggtaaatttt ccgttgtgtc
    16561 aaaaagataa gggcggttgt gattttaatg cttgccaaag cgtcgggcgg aaactataat
    16621 ccgaaactta tcgagtcgga gtgtggcgca gtctggtagc gcacttgcat ggggtgcaag
    16681 gggtcgaagg ttcgaatcct ttcactccga ccaaaaattc cgaaagccgc tttcaaaagc
    16741 ggcttttttg ccgtccgtat gattatgatg tagagtacgc ggcgacagac attcaaatgc
    16801 cgtctgaaaa ccgttcagat ggcatctctt tatcttagtt tcattccgta ccatcttaag
    16861 gaacatcaaa ttgggcattt cccgcaaaat atcccttatt ctgtccatac tggcagtgtg
    16921 cctgccgatg catgcacacg cctcagattt ggcaaacgat tcttttatcc ggcaggttct
    16981 cgaccgtcag catttcgaac ccgacgggaa ataccaccta ttcggcagca ggggggaact
    17041 tgccgagcgc agcggccata tcggattggg aaacatacaa agccatcagt tgggcaacct
    17101 gatgattcaa caggcggcca ttaaaggaaa tatcggctac attgtccgct tttccgatca
    17161 cgggcacgaa gtccattccc ccttcgacaa ccatgcctca cattccgatt ctgatgaagc
    17221 cggtagtccc gttgacggat tcagccttta ccgcatccat tgggacggat acgaacacca
    17281 tcccgccgac ggctatgacg ggccacaggg cggcggctat cccgctccca aaggcgcgag
    17341 ggatatatac agctacgaca taaaaggcgt tgcccaaaat atccgcctca acctgaccga
    17401 caaccgcagc accggacaac ggcttgtcga ccgtttccac aataccggta gtatgctgac
    17461 gcaaggagta ggcgacggat tcaaacgcgc cacccgatac agccccgagc tggacagatc
    17521 gggcaatgcc gccgaagctt tcaacggcac tgcagatatc gtcaaaaaca tcatcggcgc
    17581 ggcaggagaa attgtcggcg caggcgatgc cgtgcagggt ataagcgaag gctcaaacat
    17641 tgctgtcatg cacggcttgg gtctgctttc caccgaaaac aagatggcgc gcatcaacga
    17701 tttggcagat atggcgcaac tcaaagacta tgccgcagca gccatccgcg attgggcagt
    17761 ccaaaacccc aatgccgcac aaggcataga agccgtcagc aatatcttta tggcagccat
    17821 ccccatcaaa gggattggag ctgttcgggg aaaatacggc ttgggcggca tcacggcaca
    17881 tcctatcaag cggtcgcaga tgggcgcgat cgcattgccg aaagggaaat ccgccgtcag
    17941 cgacaatttt gccgatgcgg catacgccaa atacccgtcc ccttaccatt cccgaaatat
    18001 ccgttcaaac ttggagcagc gttacggcaa agaaaacatc acctcctcaa ccgtgccgcc
    18061 gtcaaacggc aaaaatgtca aactggcaga ccaacgccac ccgaagacag gcgtaccgtt
    18121 tgacggtaaa gggtttccga attttgagaa gcacgtgaaa tatgatacga agctcgatat
    18181 tcaagaatta tcggggggcg gtatacctaa ggctaagcct gtgtttgatg cgaaaccgag
    18241 atgggaggtt gataggaagc ttaataaatt gacaactcgt gagcaggtgg agaaaaatgt
    18301 tcaggaaata aggaacggta atataaacag taactttagc caacatgctc aactagagag
    18361 ggaaattaaa aaattaagaa cttcagaagg aaaggcaaat tctgcatcag cagctttatt
    18421 gacacttgat ttgaagacag ctcaagctgc caatcaagtt gttgaaagtt tgagaagtac
    18481 aggtaatctt ccttctaatt atattactaa atctcaggcg ataagtatgg gatggcgtcc
    18541 aggaaaagcc ttgggtaata caataaaaaa tggacaatta gggggagata tttttgcaaa
    18601 ttctacaaat attttacctg ctaaacaggg taggatatgg tatgaagcgg acattggctt
    18661 aataaatacc atgtcaagat cgaaccaggc tggtactaga ttattatatt ctaacgatgg
    18721 attgctatat ataacaactg atcattatat ctctgcaata caaattggaa cttggaaata
    18781 ggagtataac ttatgcaatt agagattatc ggtagtaaaa tttatacgga acaagatttt
    18841 cataatcaaa tttcaaaaat attttctata caagattatt atgggaacaa tcttgatgct
    18901 ttatgggatt tattaagcac aaatgtagaa cgaccgatta ctttggtatg gaaagatgct
    18961 atgttctcaa aaaatcaatt agaaaatata tttattgaaa tcgtaaatgt tctagaaaga
    19021 gttaagaaac aagatgagga ttatggattc gaagaaaaat ttaattatat tttagagtaa
    19081 gtaaacccta attacattac gtacacaggc ttaaaactcc ccagagccaa ttaagcaagc
    19141 cgtaacccat ataaaactta aactcaacaa gtagcatgtg tgcggaacgt acgcatgcgg
    19201 ttcttaaagt ttgagctaag aggccgtcta aaaacagaaa aaccgtttca gacggtcttt
    19261 gtttaacgcc accgatccag cgggttacaa agcgcagtca atgccgctgc gccttatgcc
    19321 tccgaagcaa taggcagaac atttggacac ggtgaaaaca aaaacgaaac cgcccaagcc
    19381 gtcggacatt tccttttagg agcagctatt gcccgcgtca acggtggtaa ttttgctgcc
    19441 ggcggctcgg cagcagttgc agctgaaaag gcggcggaac atcttgccca acagtataac
    19501 gacggtaaaa ccgcaatcga tccgcaaaca ggcgagttca atgccaacct gctgccggaa
    19561 catatcaaag aggaaatcaa atcaaagagc ggggtgattg catcgctgac gggcgcggcc
    19621 gtgggcggca cgccggtaga tgcgcaaacc ggaggtgcgg tcggacagaa tgcggtggaa
    19681 aacaacctct atctgacatc ggaagcctta aagaaggaca agcagacagc tcgtaaaatt
    19741 tattccgtca taaaagagca agtcaagcat gaatgcagtt ccacaggaag aattaccgaa
    19801 tgtcgtcaaa atataggacg cattatcgaa tttacccaag acaaacgctt tgacagtagg
    19861 tttaaggact taaaaaaaga atccttatat tacctaaata aacatcctga tttagtagcc
    19921 tcttatttga aggctgaata cgaaaagctg gatagggaag acaaaagtat cctgcaccgc
    19981 tacatctcac ccggggctga aatcgtttcg ggcagtttgg gggttgttct ttcaggagta
    20041 gccggaggcg gatcttgtgc cgagactttc ggcttaggct gtgccgccgc tttggttggt
    20101 gtaacgtctt cctacgatca tgtcattact gggacgaaaa acttcggaaa aaaagccagc
    20161 gagcaacgac cgacgattgc ggttcaggcc ttgaagcagt tggggctgtc ggagcaggct
    20221 gcggaatatg ttcagttctc tatagatttg ttcagtgtgg gtaaatcggg gggcggtata
    20281 cctaaggcta agcctgtgtt tgatgcgaaa ccgagatggg aggttgatag gaagcttaat
    20341 aaattgacaa ctcgtgagca ggtggagaaa aatgttcagg aaacgagaag aaggagtcag
    20401 agtagtcagt ttaaagccca tgcgcaacga gaatgggaaa ataaaacagg gttagatttt
    20461 aatcatttta taggtggtga tatcaataag aaaggcacag taacaggagg gcatagtcta
    20521 acccgtggtg atgtacgggt gatacaacaa acctcggcac ctgataaaca tggggtttat
    20581 caagcgacag tggaaattaa aaagcctgat ggaagttggg aggtgaaaac gaaaaaaggt
    20641 gggaaagtga tgaccaagca caccatgttc ccaaaagatt gggatgaggc tagaattagg
    20701 gctgaagtta cttcggcttg ggaaagtaga ataatgctta aggataataa atggcagggt
    20761 acaagtaaat cgggtattaa aatagaagga tttaccgaac ctaatagaac agcatatccc
    20821 atttatgaat agtaatattt atgaaaaatt aggagattaa tgatgaaaag aattaagtgc
    20881 ttttgtgata aatttccatc aggagataca tttagaatgt gtatcattct ggatgactat
    20941 gataataggg ttgattatta tgtaggaata tatgattaca ttacgtctac cttaatgagc
    21001 gatatttact atcgatccac gattgatgag catttcaaga ttatagaatt aatagaaaat
    21061 aatccaaatg aaatttatga tgatggcggt ggtcaacaat tttgcctaga atttcatcat
    21121 gataaggtca ttttttatca caatgaattt gatgaagaag atggttatcc agtattaagc
    21181 tgttcgctgc atacttttaa aactgcttta attgcttgga atgctttttt gcaattgcct
    21241 aaaagtattc attcggtggt ggagactgtg attgaggaat aagcataatt agcttaatga
    21301 atagaatcag cgatatagaa aggtagaatc agatatatcc cacctaaaaa ttactctcct
    21361 tcagcaccgc taccaaaagg acctaataat ggatatagtg ccgggaattt aagcaaaaaa
    21421 gtcctgactt tagtttacat aaacggcaaa ctcaaattcg ggccaagcag tctgccgtct
    21481 tcggcattga aagcacctgt tcccattgtc ggcaaggctg aggctccgcc cggaaaagct
    21541 gtgcctgtcg gcgaagcccg cattccttca aatccaaaag cggtaaaagg aacaaccgtt
    21601 accctgtcgc aggaacaacg cctagccaac ggagagctga taccgaaagg ctctgtggta
    21661 acgtggtcgg acaatgcggt gaaggttgtg cggcctgatg ggaaaaagcg gagatttaat
    21721 agtcgggatt gggaacagaa ggcattgccg aaaccgcaac taccgaaaga ttacagagat
    21781 gacatcaaca taggtggaaa taatcctaga tttaatcagc aacggcatga aaaaatcgaa
    21841 tcccacaacc caaacagaat agaggagata cgccaacgta atgccgtttc ttctgcagaa
    21901 aggcaggctg atgcattaaa aaggatgcaa acgtctggcc gcacccgcct gcctgggcag
    21961 ccacaaaaag gaaaacccgg cgctgctaga gaaatgtcct cttcagacaa tccgaatcaa
    22021 acagccgagg cttttgcacg tcgatattta ggcaggaaac ctacactaat ggaacgagtc
    22081 gaaggtgaaa aattatacgg aggtaaatgt gtgggctgtt ggaaagcgca aatgccggac
    22141 ggaacctttg tggtgtatag gccagcaggt aatgcttcta tgaccaaggg aactactgcg
    22201 tctgttgata ttaataaccc aacattaaat aacttaaata ttaatttaaa aggcaagcca
    22261 acagagttaa aatttaaatt ccctgctaga taaggatgtt ttaatgaatt tagagacagc
    22321 agaaaatatt caatgcgaat ttgagtattt agcattagat ggttattttc caatgcattt
    22381 tgcatcgcat ggtcaaggaa acaaagattg gcaatttgca gttgaattca tatatagatt
    22441 attaatatgt caattagcaa ctcttgaacc tatcaacttt gaaacgaaaa atgatatttt
    22501 agacttttgc cataatttag caaaacaaag tccttttaat aatgataatg aggtgtggta
    22561 tcagggggaa attgtattaa caaaaaaagg gatagattta ataaaggaat atattccaga
    22621 ggcgtttgaa gagtggaatg gaaagaaatt tgaattaaac attcctttca ttaaaacatt
    22681 aaaaaatata tttgttaatt atgaggtagc atgggatgag aataatccac tattccccat
    22741 tatatcttta aacctcggca cctgataaac atggggttta tcaagcgaca gtggaaatta
    22801 aaaagcctga tggaagttgg gaaatgaaaa aaggaagggg cggaaaacct atgacaaatc
    22861 attctatgtt cccaaaagat tgggatgagg ctagaattag ggctgaagtt acttcggctt
    22921 gggagaaaag aaaggaatta gaaggtcaaa aatggagagg tacaagtaaa tctggtgttg
    22981 aaatagaagg atttacggaa cctaatcgaa cagcttatcc actttataat agtaaatgaa
    23041 gtattgatta aaaaggtgtt tttaaatgta taaaataaaa tgtttttatg atcagtttag
    23101 ttctggtgaa ttatttaact actgccagtt tttagataat tcttctaatc aaaagataaa
    23161 tacacgagga acggtgtatc aatatattat ttatgtttta acaggagacc tttatctaca
    23221 aaaagacata gatgaaaatt tagaatttat ccatcaagca gaaaataatc ccaatgaagt
    23281 ttattcagga ggtggacaag gtttttgctg ggatatttct gctgaaaaag tagttttcta
    23341 tcacaacgaa tttgatgaag aagacggctg gcctgattta agctgttcgc tgcatacttt
    23401 taaaactgct ttaattgctt ggaatgcttt tttgcaattg cctaaaagta ttcattcggt
    23461 ggtggagact gtgattgagg aataagcata attagcttaa tgaatagaat cagcgatata
    23521 gattggactg caaatccacg cttatacgct gtgccatgat taacaggaag agagcaactt
    23581 ggatgggcta gtagggatgg taagcattta aatatatcaa ttgatggaaa gattacacac
    23641 aaatgactaa tttaaaatta gatttctact ctgaagttat tataaaagat tcttgcccta
    23701 atgatttgtt agaaaatggt gagactatta aaggaaaaaa aggagtggta ttaggtataa
    23761 gcgaagaaga tggtataatc tatggctata ctattttact ttttgatata aaatattgta
    23821 tatatataga taagaaatat attataccga ctggaaaaaa attctctcgc gatgattttt
    23881 attagctaat ttaagtaatt taagcaagtt ttttagatac tgaaactggt aattatgttt
    23941 atgtaagcat aaggagttca gattgaagga ttctacaggg caaatgaggc tggcaaccaa
    24001 ggatttggcg gaagccatta aacgaggaga agtacgtagt tctgctttta caacaaagca
    24061 actaaaggca atcgaaaaag gagaatctaa aataccggat tacacttggc atcatcatca
    24121 agacactgga aggatgcaat tgattaatga agacttgcat catgataccg gccatatcgg
    24181 ttggagagcg atgagtaaag gaaagtaact atgtggaaaa tcataaaaga ggatagtaat
    24241 gatttaggat ttgcaattaa atgcttattc tctcagtcta ttgatttaaa tgaattcaag
    24301 ttatggattg aacaagtaat acgagatatg cccatcgagg acatcccgtt ttatattttt
    24361 gatttggcgg attttgatgg gggaattgcc gatattgaca atattgtagg ttttgtttcg
    24421 agttacagcc tgccgaaatc gaaaaaaaat gccttgaccg gcattgcctt cttaaggggg
    24481 atagatgtct atgaccctcc cgtttcaaaa gaaaaagcat taaaagcctt agagaaatat
    24541 cctgaaattt atcagaaatt tcagcatttc tttccatttg tagaactgcc tccgctttaa
    24601 acagtcaaaa tgccgtctga aacaatattc ggctttcaga cggcattttt gacacaaagc
    24661 aggtaaccaa aagagcgttt gacggcaaag gaagataatt atgtggaaaa tcataaaaga
    24721 ggatagtgat gatttaggat ttgcaattaa atgcttattc tctcaatcta ttgatttaaa
    24781 tgaattcaag ttatggattg aacaagtaat acgcgatatg cccattgagg acatcccttt
    24841 ttatattttt gacttggcgg atttcgatgt aggaattggc gatattggca atattgtagg
    24901 ttttgctccg agttccagcc tgccgaaatc gaaaaaaaat gccttgaccg gcattgcctt
    24961 cttaaggggg atagatgtct atgaccctcc cgtttcaaaa gaaaaagcat taaaagcctt
    25021 agagaaatat cctgaaattt atcagaaatt tcagcatttc tttccgtttg tagagctgcc
    25081 tccgctttaa acagtcaaaa tgccgtctga aacaatattc ggctttcaga cggcattttt
    25141 gatacaaagc aggtaaccaa aagagcgttt gacggcaaag gaagataatt atgtggaaaa
    25201 ttacgaaaga gcaaggtcaa gatttaagtt ttgcaacata ttgcttatta tcggcagcaa
    25261 ttaatttgca agaatttaaa ttatggttgg agaaggttgt cttagatatg cctattgata
    25321 atatacattt ttatattttt gatttaattg atcttaaaga aggagtagga gatatttata
    25381 atattttgga ttttgttccg aattcagact tatctaaaga ccaagatgac gcattaacag
    25441 gcattgcctt cttaaggggt atagatgtct atgaccctcc cgtttcaaaa gaaaaagcat
    25501 taaaagcctt aaagaaacat cctgaaattt atcagaggtt tcagcatttc tttccgtttg
    25561 tagaactgcc cccgctttaa acagtcaaaa tgccgtctga aagaccgttc cgccgttcag
    25621 acggcatttt cgcccctttt gtttacaaac ccttaaaatc cctttacact caaaatccgt
    25681 tcaacatcaa acaaaccccg ctatgaaaac cctgctcctc ctcatccccc tcgtcctaac
    25741 cgcttgcggc acactgaccg gcatacccgc ccacggcggc ggcaaacgct ttgccgtcga
    25801 acaggaactc gtcgccgcat cgtcccgcgc cgccgtcaaa gaaatggact tgtccgccct
    25861 gaaaggacgc aaagccgccc tttacgtctc cgttatgggc gaccaaggtt cgggcaacat
    25921 aagcggcgga cgctactcta tcgacgcact gatacgcggc ggctaccaca acaaccccga
    25981 aagtgccacc caatacagct accccgccta cgacactacc gccaccacca aatccgacgc
    26041 tctctccagc gtaaccactt ccacatcgct tttgaacgcc cccgccgccg ccctgacga
//
LOCUS       DAWHSK010000006        25679 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.006, whole genome shotgun sequence.
ACCESSION   DAWHSK010000006 DAWHSK010000000
VERSION     DAWHSK010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 25679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25679
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.006"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(951..1619)
                     /locus_tag="WIC03_000164"
     CDS             complement(951..1619)
                     /locus_tag="WIC03_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ3882727.1"
                     /translation="MKNLAIFDLDNTLINTDSDHAWPQYLIKKGLVDAAETEAQNEKF
                     YRDYQNGCLDIDAFLKFHLAPLARYSKEELAEFHREFMAEYIIPHISPMQRMLVQSHQ
                     MAGDETLVISSTNEFIITPVCHLFGITNIIGTQLETGSDGRYTGNYIGTPSLKEGKIT
                     RLNQWLAERGETLQSYGKTYFYSDSKNDLPLLRLVSEPVAVNPDAELEKEAKEKGWPV
                     LNFK"
     gene            complement(1616..2284)
                     /gene="hda"
                     /locus_tag="WIC03_000165"
     CDS             complement(1616..2284)
                     /gene="hda"
                     /locus_tag="WIC03_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HEZ3882728.1"
                     /translation="MNQLIFDFAAHDYPSFDKFLGTENAELVYVLRHKHGQFIYVWGE
                     EGAGKSHLLQAWVAQALEAGKNAAYIDAASMPLTDAAFEAEYLAVDQVEKLGNEEQAL
                     LFSIFNRFRNSGKGFLLLGSEYTPQQLVIREDLRTRMAYCLVYEVKPLTDQEKIDALA
                     SMAAARQVTVDSEIFEYLLKHWRRDMDSLMMMLDTLDNYAVTMGKRITLPLLRQLLKQ
                     QETQ"
     gene            complement(2389..2940)
                     /locus_tag="WIC03_000166"
     CDS             complement(2389..2940)
                     /locus_tag="WIC03_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ3882729.1"
                     /translation="MSGININLKNGDSLLIRGPSGCGKTSLLRALAGLWPFGSSGKVS
                     RPPHQDILSLPQRPYTAQGSLRDAVCYPDIDKQHPELAEAMNTCRLGYLVDKLDKTDD
                     WQHKLSPGELQRVAFVRALLSKPKIVLLDEATAALDEPTEALLYRALKQKLPDSIIIS
                     IGHRSTLNAFHNMHLDVGNAACG"
     gene            complement(3189..3908)
                     /locus_tag="WIC03_000167"
     CDS             complement(3189..3908)
                     /locus_tag="WIC03_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ3882730.1"
                     /translation="MDTFLDRLKFLWPHGAKPSDIYNKIEMSASGFNRVWKEGAIPTA
                     DYLVKIQEVTGCDLNWLLTGKGLPYLDKARPENAGAFSVSRNSDGITDTMGNPVDLSE
                     FVFVPRYNVAAAAGYGAPVFGEEPLFCLAFRKYWIDNYVTKQPEKLSVITVKGDSMEG
                     VLNHGDSILINHAETDPRDGLYVLRIGNDLFVKRVQRIPGKLLVTSENPRYAPFEIDL
                     SNAQDDIAIIGRVEWYGRSID"
     gene            4130..4333
                     /locus_tag="WIC03_000168"
     CDS             4130..4333
                     /locus_tag="WIC03_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEZ3882731.1"
                     /translation="MKKKQESMTDWHRADIVARLKKAGWSVRALSIEANLAPNTLGKA
                     LDAPYLKGERIIAAAIGVPAEEI"
     gene            4455..6443
                     /locus_tag="WIC03_000169"
     CDS             4455..6443
                     /locus_tag="WIC03_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ3882732.1"
                     /translation="MKISASDIAKLGIPSLPTDRQGIEYHAKKNNWQHCFEQKGRGRP
                     KKLYEIASLPAEIRAAIMKRQSDELAEKMPKMLPQVRPGTAMSAQALAEAAKLLNEKQ
                     RSVADARCAVVAAVLGIKYEYDCSAKAAVAQFLGLLAEGKLDAVTLGNLEKANDRSRS
                     AKVGERTLDGWISAYLKAENAIERLVALAPKTTKAVKPIESYGWLPMFMRFHNIPSAP
                     KLAHSYRRFVQWAEAENMPVNDVPNLSMVRRVWDKLPLIMQERGRKTGAAYKSLLPYV
                     KRDWGALKPNDVWIGDGHSFKAKVAHPVHGRPFKPEVTVIIDGCTRFVVGFSVSLAES
                     CVAVSDALRIGVKHFGLPIIYYSDNGGGQTGKTIDHEITGITSRLGIRHETGIAGNPQ
                     GRGIIERWWKDNLIEMARQYETFAGAGMDSSTKNLMYRKMESAFNALEKGKDLTEEQQ
                     KYLKKLPSWSRFIADVVKCIDDYNNRPHGELPRHPDGGHYSPKAYREIRLEQDGIAPD
                     MLSAEELATMFMPQEVRKVQRGWLDLFSNSYFSTELAEYHKDEVRVSYDLSDASAVNV
                     FDMDGKFITKAQANGNTREAFPTARIDQLAEKRRKGKIKRAENAIKLANAEVNPALEQ
                     AAAWDELGHLGGNVIEAEYAVLPKTGTDDGIFLFEADM"
     gene            complement(6510..6767)
                     /locus_tag="WIC03_000170"
     CDS             complement(6510..6767)
                     /locus_tag="WIC03_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEZ3882733.1"
                     /translation="MKEFPDLEKRLIMLEVAVQDLEERSLTDSFVLAWLLQRITRQEP
                     TSIEQVRSFLQAQAKTFEPDSVQREHLESLLEIVESAQGLA"
     gene            6813..7985
                     /locus_tag="WIC03_000171"
     CDS             6813..7985
                     /locus_tag="WIC03_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEZ3882734.1"
                     /translation="MKQINQALQQKLVEFKEKSGMNQTQLARGIDTSPASISMYLNGT
                     YAEKGGNYETIETKIEAFLEMQKSKARREELVLGFVATKTTRRIAEVMRNAHEGGETV
                     VIYGQAGLGKTQAVKNYCEKNPAAILIEANPSFTALVLMRKLATAAKVSAMGSLNDLF
                     ESVSDRLRDSGRLIVVDEAENLPLRALEIIRRLHDETGCGLVLSGMPRLVANLRGKHG
                     ELVQLYSRVSVALNLGESLPDDELFEIAKAALPDAVKHLLPDSVQALITVIGFNETLE
                     LVRLMGGTTYPLRQGYTKNSQSRVAYLEEIIGSEAAGRLVEAMAPCNLFIPRCETALY
                     ELRNRKIRSQFDRQTAGGTPAYEAVNDLALAHRLSDRHVWRILKQADKEAEQENLF"
     gene            7987..8508
                     /locus_tag="WIC03_000172"
     CDS             7987..8508
                     /locus_tag="WIC03_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3882735.1"
                     /translation="MECHADVWHFILEKNMKKFYFVLLALGLAACGQEQSQKADAEQY
                     FFANKYQFADEKQAFYFERAARFRVLQQGLGGDFERFLKGEIPNQENLAKYRENITQA
                     VAYYADTNGDDDPYRVCKQAAQDAEILMKSMVTSGGGGTTDLDKESYQNYRKSMQECR
                     KTITEAEANLPKK"
     gene            8651..9196
                     /locus_tag="WIC03_000173"
     CDS             8651..9196
                     /locus_tag="WIC03_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEZ3882736.1"
                     /translation="MSKIICLTAGHSNTDPGAVNGSDREADLAQDMRNIVASILRNDY
                     GLTVRTDGEGKGNMPLRDAVKLIRGSDVAIEFHTNAAANKTATGIEALSTPKNKRWCQ
                     VLGKAVAKKTGWKLRGEDGFKPDNAGQHSRLAYAQAGGIVFEPFFISNDTDLALFKTT
                     KWGICRAIADAIAMELGATKV"
     gene            9193..9396
                     /locus_tag="WIC03_000174"
     CDS             9193..9396
                     /locus_tag="WIC03_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3882737.1"
                     /translation="MKKSLIALCVAHCAKLKNDFGVPPLPEIKITPSPVRVGSLKQHP
                     SLRLGKSGVAAAKRAARKRKNRH"
     gene            9399..9563
                     /locus_tag="WIC03_000175"
     CDS             9399..9563
                     /locus_tag="WIC03_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3882738.1"
                     /translation="MGQVAFYEKMIGLWSAKSREASEQADLAAFEFAEGELANYQEML
                     KRHLQTKSVE"
     gene            9566..9802
                     /locus_tag="WIC03_000176"
     CDS             9566..9802
                     /locus_tag="WIC03_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3882739.1"
                     /translation="MRILDIFKNPATGNVSHSKLWANVACAAGTVKFVMLPDPSAEIW
                     AVYLGIVGGYAVARSLVSVKRQEVENESRETAGE"
     gene            9774..10193
                     /locus_tag="WIC03_000177"
     CDS             9774..10193
                     /locus_tag="WIC03_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3882740.1"
                     /translation="MNLVKLLANNWQPIAIIALVGTLLAVSHHQGYKSAFAKQQAVID
                     KMEKDKAQALLLSAQNYARELEQARAEAKKYEVKAHAVGMALAKKQAEVSRLKTENKK
                     EIENVLTQDRKNAGGGCIDGFGHHGLQLYKRALGYGN"
     gene            10090..10395
                     /locus_tag="WIC03_000178"
     CDS             10090..10395
                     /locus_tag="WIC03_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3882741.1"
                     /translation="MSLLKTVKMQAAVVLTALAITACSSTSAPSATEIKVVEKAVMPT
                     PPAALMVAPVRPNPPKDGKTATLLEHAAEFGGYVAELENQNQAWRDWAGNHSRKVGN"
     gene            complement(10440..10610)
                     /locus_tag="WIC03_000179"
     CDS             complement(10440..10610)
                     /locus_tag="WIC03_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HEZ3882742.1"
                     /translation="MYFEIYKDAKGEYRWRLKAANHEIIAQGEGYTSKQNCQHAVDLL
                     KSTTAATPVKEV"
     gene            10623..10841
                     /locus_tag="WIC03_000180"
     CDS             10623..10841
                     /locus_tag="WIC03_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3882743.1"
                     /translation="MNEYTFSYRFNGKSWSLSIWADNPEEARAKFRAARENAHYDGEV
                     VAKVYTFVNISWVKKLYKRTKYLMGIKE"
     gene            10838..11176
                     /locus_tag="WIC03_000181"
     CDS             10838..11176
                     /locus_tag="WIC03_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3882744.1"
                     /translation="MTYRELVERQLAVRHADLELGLSRAREQEPFVIHVSDLLDKAGI
                     EYAVRMDKDFQTTFHLEYPITNYDTFKRAVWQTLGAYYCVCNDGDGLEIASNRPDGYA
                     VRIVFGDVPV"
     gene            11188..11454
                     /locus_tag="WIC03_000182"
     CDS             11188..11454
                     /locus_tag="WIC03_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3882745.1"
                     /translation="MDFEFGFRTLWPIATAAFWFWVNGISGRLKEADKRIDDLKEELH
                     AVKLSYHTKADAKADSTNIAAALERIENKLEKVNEKLDRKADKS"
     gene            11451..11648
                     /locus_tag="WIC03_000183"
     CDS             11451..11648
                     /locus_tag="WIC03_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3882746.1"
                     /translation="MSDPILEALARIENKTDQTLKNQKEMQAEIAQIRQDTKRTAITF
                     GALGGGVITVGWELLKAKMGL"
     gene            11651..12157
                     /locus_tag="WIC03_000184"
     CDS             11651..12157
                     /locus_tag="WIC03_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1804 family protein"
                     /protein_id="HEZ3882747.1"
                     /translation="MAHPQEIREKLRRLYVSGEQTLETAALMCEIPQTTARAWKRADK
                     EKGDDWDKMRAAYTLAGGGIEDLSRAMLAGFMVQYNSTMTMLQDSSTEDLPPSDRAKL
                     LASLADAFTKTVSANARVMPETSKLATALELIEFLMAFVQEKHPKHLPAFVEVLEPFG
                     AEVEKKFG"
     gene            complement(12154..12666)
                     /locus_tag="WIC03_000185"
     CDS             complement(12154..12666)
                     /locus_tag="WIC03_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3882748.1"
                     /translation="MTGFISNAFIGLKEAGSLLKGFNQLKTEAEVAQKTIELNQIIAG
                     IQTDLFEAQSSYTAAISRIDELEKELVAVKNWSTEKQRYQLYELAPGSFVYRLKTEMA
                     NSEPIHDICPQCYEQGVKSILQFNGYEGAFHKYSCSRCQTIILGERLKSDVEVFSVGG
                     SRYGRLIDGY"
     gene            12678..14300
                     /gene="terL"
                     /locus_tag="WIC03_000186"
     CDS             12678..14300
                     /gene="terL"
                     /locus_tag="WIC03_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HEZ3882749.1"
                     /translation="MKTKEFLKSLAELAASLRQVIEAEVDGFDASPKAIAARRAKVFD
                     PVGGYEYFVNTYFPHYIRSPEKSELHAFLFSRLPEIIRSPKGENEAVGAPRGEGKSTQ
                     VTQLFTLWCIVTGQKHYAVIVMDSIDQAYPMLEAIKAELEFNPRLKTDFPEVCGQGRV
                     WQAGTIVTANDVKVQVAGSGKKLRGLRHGPYRPDLTILDDIENDEQVRNPEQRDKLNA
                     WLTKTVLPLGGVGQKYDVIYIGTILHYDSVLNRTLNNPFWHGIKFKAMKRWPDRMDLW
                     DRWEELFRNDGETVAEAFYLANKDEMERGAQTSWAARGVLALMKIRARDGHATFDSEY
                     QNDPVSGEDAPFAKSMKFWNDLPSDLVYFGALDPSLGKAGASRDPSAIIIGGYQRETG
                     KLYVVEAQIKKRLPDLIIEDVIRLHRQYRCKLWFVETVQFQEFLKDELVKRSAARGIP
                     VPARAVKPVSDKLLRIETLQPHMANGLILLNESQQTLIQQFRHFPKADHDDGPDAVHM
                     LWSGAVANCVPIEWQSPTDNDFDDEIKSKWSR"
     gene            14300..15868
                     /locus_tag="WIC03_000187"
     CDS             14300..15868
                     /locus_tag="WIC03_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 domain-containing protein"
                     /protein_id="HEZ3882750.1"
                     /translation="MAKKNNKTKIQKPEAALQTDVAQITATGRVIAEHPSNFITPQKM
                     RALFEDAESGDIRAQHELFADIEERDSDIAANMGTRKRALLTLNWRVAPPRNATPEEE
                     KLSDQAYEMMDSLPTLEDLIMDLMDAVGHGFSALEVEWVFSDGLYLPRNFIHRPQSWF
                     KWDKDNGLLLRTRENPEGEALWPLGWVVHTQKSRSVQQARNGLFRTLSWLYMFKHYAV
                     HDFAEFLELYGMPIRIGKYGAGATKEEKNTLLRAVAEIGHNAAGIMPEGMEIELHNAA
                     SGTMATSNPFLQMADWCEKSAARLILGQTLTSGADGKSSTNALGNIHNEVRRDLLVSD
                     AKQVAQTITSQIIGPFLQINYPNADPNRVPKFEFDTREPKDIAVFADAIPKLVDVGVQ
                     IPESWVRDKLVIPDVQEGEAVLVRQVPDNPINRTALAALSAHTVPSKATGRHQEILDG
                     ALDDALVEPDFNSQLNPMVRQAVAALNACNSYEEADAALNALYPNLDNAKLRTYMQQA
                     LFISDILGQDHARA"
     gene            15855..17180
                     /locus_tag="WIC03_000188"
     CDS             15855..17180
                     /locus_tag="WIC03_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HEZ3882751.1"
                     /translation="MPAPDLGFALSLPPKKAIEWLESKKVTAESYRNLTASEIAKVYT
                     IARMTDLDMLNDIKTSMVESAKSGQSFDDWRKGILNLLGNKGWLHPNGHNGKDIIDPA
                     TGEVFGSPRRLETIYRTNMQTAYNAGQYQGYMANIDARPYWMYDAVGDSRTRPAHSAI
                     DGLVYRYDDPFWATFYPPNGYNCRCSVIALSERDVERQGRIVGQSTADNLVETHKIYN
                     KKGDTYLTLAYKAPDGSLYTTDRGFDYNAGRMNYRPDLDKYDRALAHQFAKAEMGGAD
                     FKTSFKQLEKEFYEVKQRLDIDGKPDKEQKIKIRNALSRQLKFAAGVLSKETQELAGM
                     TRATVWLSDDTLVKQVDSREGQSFGTDLYAMLPDLIHEPEYIFYDKSRGKESFVLLGK
                     PLEGNEKYRLMAVLKYLPQYDSIFLDSFRRADEKEIVKSKKRYEMKKGY"
     gene            17308..17724
                     /locus_tag="WIC03_000189"
     CDS             17308..17724
                     /locus_tag="WIC03_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HEZ3882752.1"
                     /translation="MIDVKIDNIFVVLNQIERLGNGIENRYLLMRRLSETMHTAVKLN
                     FRYAGRPKWLGLKYRDGKPLSDSGRLKDSFSTLSDNDTALVGTNIVYAAIHNFGGMAG
                     RNRKVRIPQREFLTLTDDDKQALMDDVQDYFSGLIP"
     gene            17955..19016
                     /locus_tag="WIC03_000190"
     CDS             17955..19016
                     /locus_tag="WIC03_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3882753.1"
                     /translation="MSKNAQKTLLAVCSFEVQPKDGRIQLLPYGEFRAVDGRPTDVPA
                     WYLTEENGHDVALLANSSRNQLVVDYEHQTLYKEKNGQPAPAAGWMRWLEFTPKGMFA
                     EVEWTDKAAAAIAAKEYRYISAVFSYDTKGYVSKIFHAALTNFPALDGMDEVLAAASA
                     QILKPETEQNPMKELLQQLFGLPDAGEEELKAALSALVEAKPEGAALSADVFAQLAEK
                     DSRIAALSAQNGTPDLTRYAPVSLVRDLQNQVAALTAEREADKGGELITAALSAGKLL
                     PAQKEWAEGVLKQPGGFAFLSGFIENAQPVAALAGSQTGGKAPDERVAALTAEEAAAA
                     KMLGMSGEEFVKIKESEGK"
     gene            19117..19587
                     /locus_tag="WIC03_000191"
     CDS             19117..19587
                     /locus_tag="WIC03_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3882754.1"
                     /translation="MQPVAQAVRLLSTSSLLSVATALIEAHGEEMTAPDLIEVNRAMR
                     RRMQAEIAALRAVQTAAAESGGLTANAVYTEAYQTAESLRAAAGSLNALVAAAINQKP
                     PLIVRQAPIDGTIHQIAHEFYGDIARAAELVRLNPHIHHPAFIKRGTLVNSYAK"
     gene            19577..20722
                     /locus_tag="WIC03_000192"
     CDS             19577..20722
                     /locus_tag="WIC03_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEZ3882755.1"
                     /translation="MQNNSYGYAVSVRVGGKEHRHWERYDIDSDFLIPADSFDFVIGR
                     LGPEAAIPDLSGESCEVVIDGQIVMTGIIGSQRHGKSKGSRELSLSGRDLAGFLVDCS
                     APQLNVKGMTVLDAAKKLAAPWPQIKAVVLKAENNPALDKIDIEPGETVWQALTHIAN
                     SVGLHPWLEPDGTLVVGGADYSSPPVATLCWSRTDSRCNIERMDIEWDTDNRFSEVTF
                     LAQSHGRSGDSAKHDLKWVYKDPTMTLHRPKTVVVSDADNLAALQKQAKKQLADWRLE
                     GFTLTITVGGHKTRDGVLWQPGQRVHVIDDEHGIDAVFFLMGRRFMLSRMDGTQTELR
                     LKEDGIWTPDAYPKKAEAARKRKGKRKGVSHKGKKGGKKQAETAVFE"
     gene            20719..21387
                     /locus_tag="WIC03_000193"
     CDS             20719..21387
                     /locus_tag="WIC03_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HEZ3882756.1"
                     /translation="MSLSKLAKKTAQTAKNIGETLRTAFRGKITLVVSSEPIQRVQLS
                     GLADETLQDLEHLQEYGFASHPPDGSEAVVIPLGGNTSHGVIVCSQHGSYRIKNLKPG
                     ETAIFNHEGAKIVIKQGKIIEADCDVYRVNCKQYEVNAATDAKFNAPLVETSAVLTAQ
                     GQINGNGGMAVEGGDGATFSGDVNQTGGSFNTDGDVVAGNISLRQHPHTDSIGGKTLP
                     AEPA"
     gene            21488..21835
                     /locus_tag="WIC03_000194"
     CDS             21488..21835
                     /locus_tag="WIC03_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HEZ3882757.1"
                     /translation="MDKELNPSIGDYTGRTVDTLQNAVYIRLMTPLGSWWADKTLGSL
                     LHLLQREKDLQRVSLLAEQYADEALQPIVKSGRADKITVRAEQPHDGRLILHIRVDTA
                     AGGFDYRHEVPVI"
     gene            21849..22904
                     /locus_tag="WIC03_000195"
     CDS             21849..22904
                     /locus_tag="WIC03_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HEZ3882758.1"
                     /translation="MFETPTFEQIRERILRDTKSLWPDADISPDSDHYVHASRLASCA
                     EGQYAHQSWIVRQIFPDTADREYLERHASMRGLSRRNPTTASGTLTVSGIAQSMLSDD
                     LQVRIGQRFYRTTARAVIGSGGTAEIPAIADEPGAAANVRDGEAQLMAAPAGVATECR
                     LTVQGGTDRESDASLLARLLEIIRRPPAGGNRYDYKNWALSVDGVTSAYVYPLRRGLG
                     TVDIAITSADGVPSEETVRRVQAYIDEMRPVTAKNALVLKPTVTAVPVTVQVKLDGID
                     LDEAKRRIRTALKEYFDTLIPGDGLTVSQIEAAISNVDGVIDRRLTAPTANRAADTVN
                     RIEWFKAGAINVTEMPS"
     gene            22901..23461
                     /locus_tag="WIC03_000196"
     CDS             22901..23461
                     /locus_tag="WIC03_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HEZ3882759.1"
                     /translation="MSYQDILRGLLPPVSYARNAPRVRAQAEIDGAALDAVAESAQSV
                     ADAVDPRSAGQMLADWERVLGLDGTGKNRQRRVLAVMAKLNETGGLSIPYFVRLAEAA
                     GYQIQIDEPQPFRAGVNRAGDRLAPQEIMWVWHVNVRGGNNRITRFRAGISAAGDRLT
                     DYSDAVIESLFNRLKPAHTAIRFTYR"
     gene            23472..25448
                     /locus_tag="WIC03_000197"
     CDS             23472..25448
                     /locus_tag="WIC03_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HEZ3882760.1"
                     /translation="MHPIETPDKTFHDGDGVSELGTILPAWWLNQVQSELLAVLTAAG
                     IQPDKSQPNQLLAALNRLAVVTTGDQTITGQKTFTAQTQFQSGIHLSANQTNWNGGHK
                     AYIGADADNAHIVFGDDTLRLHSANNRISYNNHDIFHKANKPRFAEDIEGKPNTLSGY
                     GIGNFKVETFRGDLNTLKTDGIYSLPTAVGSSNLPVENTACHIQVIAGTKHGWCRQLG
                     YPAYTSDVYERHQTSSANDNWSAWKKLNSDGIPVGAIVSFPKAVQNPAGYLKANGTTF
                     AQNTFPDLYRALGNSNRLPDLSRTDIGITAWFPSDQIPTGWLAFDDIRTRVTETAYPE
                     LYRLLTGKYGSIQNVPQAEDRFIRNAGNGLAVGTKQEDEIKRHVHKVFSHWTNHTDAA
                     AVGYEDHNERQRSALVSTWTDGDLNDNGFLTPRLDSKMATGGAENRPKALVLKLCIKA
                     ADTLGEAVFWIKSHGETINAGALDAGTLAQNLQDKADRAHTHTAAQIQGLDEKISTAV
                     AAQFTRQTIGGVDIVRFPDGTMIQTGSYRFTRSGGPIENEVVFPVAFADGNVKCFVSE
                     RHSGRVTGDRRQHNWLFIRAKNHAAAIITNWYEGSCDWMAIGKAASGNAASSPIGPEI
                     PETNEEPQRESGRTSTGPRNRRRHRDGLLEALQD"
BASE COUNT         6590 a         6250 c         7209 g         5630 t
ORIGIN      
        1 tgatttttgt taatccacta tacgtcctag attcccactt tcgtgggaat gacgggatgt
       61 gggtttttgt gcggatttga accggtaagg gtggtgtggg attggtggtt tgcttaggat
      121 cttttggatt gtagtttgta tatacattta cttgttgata aaagataaaa taaaattaga
      181 aactaaaagt gagaaaaaat taataataat agggatgtat aaatgtaaag gctccgtttc
      241 atagctaagg ttatctgaat atatggaaaa aaagtaaaag tccataaaac taaaatatat
      301 agataatgct aatgataata gaattatcac tttcctagag taaggataat atttttttat
      361 cgtaaaaaaa tataaaatag gataaataat tgccatatag atagattttg taacaaaatt
      421 aaaaataaat aataaaaata atgtaataaa gtatattttt tggctatgaa ataaaattgt
      481 acataattga acgagcagat caaaaaatga actacatata acaataaata ataacatatt
      541 taccatacta aatttaatag gtctcattat catatttaat aaccacttca tagtaaatgc
      601 agagtgggtg gaaacactca ctttatggtt tgctacgctc tgctcaatta gcaacccgat
      661 aacccaatat ggataatagg gtaattaatc caatctaatt tgtcagcatc cgttaattta
      721 ttgcaaaata aagtattgaa ttatgtcggg tgcaaatgac gaaatataag tttccgtgcg
      781 gacggattaa gattcccact ttcgtgggaa tgacggtgga aagattgttg tttttcccga
      841 tgaattcctg tgttttttgt ttttccggat aaattcctgt ggctttgagt tttttggatt
      901 tcagcctcaa tgccgtctga acgccgaatc gggcttcaga cggcattgcg tcatttgaaa
      961 ttcaaaaccg gccagccttt ttctttggct tctttttcca gctcggcatc ggggttgacg
     1021 gcgacgggtt cgctgacaag gcgcagcagc ggcaggtcgt ttttggagtc gctgtaaaaa
     1081 taggttttgc cgtagctttg gagcgtttcg ccgcgttcgg caagccattg gttcaggcgg
     1141 gtgattttgc cttctttgag gctgggcgtg ccgatgtaat tgccggtgta gcggccgtca
     1201 gaaccggttt cgagttgtgt gccgatgatg ttggtgatgc cgaaaaggtg gcagacgggg
     1261 gtgatgatga actcgttggt tgaggaaatc acaagggttt cgtcgcctgc catttggtgg
     1321 ctctgcacca gcatacgctg cataggcgag atgtggggga tgatgtattc cgccataaat
     1381 tcgcggtgaa actctgccag ctcttctttg ctgtaacgag cgagcggggc aaggtggaat
     1441 ttgaggaatg cgtcgatgtc gaggcagccg ttttggtagt cgcggtagaa tttttcgttt
     1501 tgcgcttcgg tttcggcagc gtcaaccaag ccttttttga tgaggtattg cggccaggcg
     1561 tggtcggaat cggtgttgat gagggtgttg tcgaggtcga agatggcgag gtttttcatt
     1621 gggtttcctg ttgtttcaaa agctggcgca aaagcggcag ggtgatgcgt ttgcccattg
     1681 tgacggcgta gttgtccagc gtgtcgagca tcatcatcag gctgtccata tcgcgccgcc
     1741 agtgtttgag caggtattcg aaaatttcgg aatcgacggt tacttggcgt gccgccgcca
     1801 tactggcgag cgcgtcgatt ttttcttggt cggttaaggg tttgacttcg taaacgaggc
     1861 agtacgccat acgcgtccgc aaatcttcgc ggatgacaag ctgctggggc gtgtattccg
     1921 aaccgagcag caaaaagcct ttgccgctgt tgcggaagcg gttgaagatg gaaaaaagca
     1981 gggcttgttc ttcgttgccc agtttttcga cttgatcgac ggcgaggtat tccgcctcga
     2041 acgcggcatc ggtcagcggc atggaggcgg catcgatata ggcggcgttt ttgccggctt
     2101 cgagcgcctg tgcgacccac gcctgcaaaa gatggctttt gcccgcgcct tcttcgcccc
     2161 aaacatagat aaactgtccg tgtttgtgtc ggaggacata gaccagttcc gcgttttccg
     2221 tgccgaggaa tttgtcgaaa ctcggatagt cgtgtgcggc aaagtcgaaa ataagctggt
     2281 tcacggttcg gcattccgag gggtggtaaa cgggtttatt gtacgttgtt ttcgcgcgcc
     2341 tttccaattt gaacgatgcc gtctgaaaac ggcttcagac ggcatcgttc agccgcaggc
     2401 agcgttgccg acatcgaggt gcatattgtg gaacgcgttg agcgtgctgc ggtggccgat
     2461 gctgatgatg atgctgtcgg gcagtttttg tttcagtgcg cggtagagca gggcctcggt
     2521 cggttcgtcc aaagcggcgg tggcttcgtc gagcaggacg attttgggct tggaaagcag
     2581 ggcgcggacg aaggcgacgc gttgcagttc gcccggggag agtttgtgtt gccagtcgtc
     2641 ggttttatct aatttatcaa ccagataacc caagcggcag gtgttcatgg cttcggctaa
     2701 ctcgggatgc tgcttgtcaa tgtcggggta acaaaccgcg tcgcgcaggc tgccctgtgc
     2761 cgtgtacggg cgttgcggca gggagaggat gtcttgatgc ggcggacggc tgactttgcc
     2821 gctgctgccg aacggccaaa gccccgccag cgcgcgcaac agcgaggttt tgccgcaacc
     2881 gctcgggccg cgtatcagca gggaatcgcc gtttttgagg tttatgttga tgccgctcaa
     2941 caggatttcg ccgttgtggc agaacagagc gacgttttcc tgtgggggac tgttagtttt
     3001 tgcacaagga acaaatagag taaaaaaacg ctgaaatctt cggaagacgt ggatttcggc
     3061 gtttttttgt atccggaaaa gttacgccag ctttttcaca aaaccgcgcc ggaatgcgcg
     3121 gttttctgtt taaagctgac gagattaggg aatttttaaa actgttttaa gaggttttta
     3181 aaatggattt aatcaatact ccggccatac cactcaacac ggcctatgat ggcgatgtcg
     3241 tcttgggcat tgctcaaatc tatttcaaac ggtgcgtaac gtggattttc agacgttaca
     3301 agcagtttgc ccggtatacg ttgcacacgt ttgacaaaga ggtcattgcc tatacgcaag
     3361 acatataggc cgtcacgcgg gtcagtttcg gcgtggttga tgagaatgga atccccatga
     3421 ttgagcacgc cctccattga atcgccttta acggtaatta cagacagttt ttccggctgt
     3481 ttggtcacat agttgtcaat ccaatatttc cggaaagcca agcagaataa aggttcttcg
     3541 ccgaagactg gtgcgccata ccctgctgct gcggctacgt tgtagcgcgg cacgaataca
     3601 aactcggaca ggtcgacagg attgcccata gtgtcggtga ttccatcaga atttctactt
     3661 acagagaatg ctccggcgtt ttccggcctg gctttatcga gatacggcaa gccttttccg
     3721 gtcagcagcc agtttaaatc acaaccggta acttcttgaa tttttactag gtaatcggct
     3781 gttgggatag ctccctcttt ccaaactcta ttaaatccag aagccgacat ttctattttg
     3841 ttatagatgt cagatggctt agccccatga ggccaaagaa atttgagcct atctaaaaaa
     3901 gtatccatag taatcctaat ttaactcatt taagcaaaac attaagcaaa aaaagaaact
     3961 cttttgctta aataagatta ctcaaataat caatattttg taaaaataat tacgtttttg
     4021 agaaaatatt ttagcaaaag agtttcatga agctgttttg ctaaatgtaa ttcactcatt
     4081 tgctaaatga cggcggttaa taaacctact taattaagga attgcgagaa tgaaaaaaaa
     4141 gcaagaaagc atgactgatt ggcatcgtgc tgacattgtg gctcgtctta aaaaggcagg
     4201 ctggtctgtg agagcgttat ctatcgaggc taatttagca ccaaatacat tagggaaagc
     4261 tttagatgct ccttatctga aaggcgaaag aatcattgca gcagcgattg gagtacccgc
     4321 agaagaaatc tagccatctc gttttgagaa acgaaaccat aagccaacct tcccaagatc
     4381 tataaataga taactgtttt gctaaatagt tccaaaagag taccgcattt aagcaaaaat
     4441 agaaagcgga aaaaatgaaa atatctgcat ctgatattgc gaaattagga attccgagcc
     4501 taccaactga tagacaaggg attgaatacc atgccaagaa aaataattgg caacactgtt
     4561 ttgagcaaaa aggaagaggc cgtcctaaaa aactgtatga aatcgcttcc ctccctgccg
     4621 aaatccgagc agccatcatg aaacggcagt cggacgagct ggcggagaag atgccgaaaa
     4681 tgctgcccca agtcagaccg gggacggcga tgtcggctca ggcactggct gaagcggcca
     4741 agctgttgaa cgagaaacaa cggtcggtgg cggatgcgcg atgtgcggtg gtggcggcgg
     4801 tattggggat taaatacgaa tacgattgct ctgccaaggc tgcggtggct cagtttttgg
     4861 gcttgctggc agaaggtaaa ttggacgcgg tcacgcttgg gaacttggaa aaggccaatg
     4921 accgcagccg gtcggcgaag gttggcgaac gtactttaga cggctggatt tctgcttatt
     4981 tgaaagcgga aaacgcgata gagcggttgg ttgctttggc tccgaagacg acgaaggcgg
     5041 tcaagccgat tgagagctac ggttggttgc cgatgtttat gcggtttcac aacatccctt
     5101 cggctccgaa actggcgcac agctaccgcc ggtttgtgca gtgggcggaa gcggaaaata
     5161 tgccggtcaa tgatgtgcct aatttgagta tggtgcggcg cgtttgggac aagctcccgc
     5221 taattatgca ggagcgcggc aggaaaacgg gggcggctta taaatcgctg ctgccttatg
     5281 tgaaacgtga ttggggggct ttgaagccga acgatgtttg gatcggcgac ggccacagct
     5341 ttaaggcgaa ggtggcacac cctgtacacg gcaggccgtt taagccggaa gtgacggtga
     5401 ttattgatgg ttgtacgcgg tttgtggtcg ggttttcggt ttcgttggct gaaagttgtg
     5461 tggcggtatc ggatgctctg cgtatcgggg tcaagcattt tggtttgccg attatctatt
     5521 actcggataa cggcggcggc caaaccggca agacgataga ccatgaaatc acgggtatta
     5581 cgtcccgact gggtatccgc catgaaacgg gtatcgcggg caacccgcaa ggtcgaggca
     5641 tcatcgagcg atggtggaaa gacaatctga ttgagatggc gcgccagtat gagacgtttg
     5701 cgggcgcagg aatggacagt agcacgaaga atctgatgta ccgcaagatg gaaagtgcgt
     5761 ttaacgcttt ggaaaaaggc aaggatttaa cggaggaaca gcaaaaatat ttgaaaaaac
     5821 tgccgagctg gtcgcgtttt atcgcggatg tggtcaagtg tatcgatgat tacaacaacc
     5881 gtccgcacgg cgagctgccc cgacatccgg acggcgggca ttattcgccg aaggcttatc
     5941 gggaaataag gctggaacag gacggtatcg cgccggatat gttgtcggcg gaagagctgg
     6001 cgacgatgtt tatgccgcag gaagtgcgaa aggtacagcg cggttggctg gatttgttca
     6061 gcaactctta tttctcaacc gagctggcgg agtatcacaa agacgaggta cgggtcagct
     6121 acgatttgag cgatgcgtcg gcggtcaatg tgtttgatat ggacggcaag tttattacta
     6181 aggcgcaggc caacggcaat acccgcgagg ctttcccgac agcgcgtatc gaccaactgg
     6241 cggaaaaacg ccgaaaaggc aaaatcaagc gggcggaaaa tgcaatcaag ctcgcgaatg
     6301 cggaagtcaa tccggcactg gaacaggcgg cagcttggga cgagctggga catttgggcg
     6361 gaaacgtcat cgaggcggag tatgcggtat tgccgaaaac gggcacggac gacgggattt
     6421 tcttgtttga ggcggatatg taaaggaaaa catgatggaa aaacagaaaa atgcggcgtt
     6481 ttcggccgag cttgttgaaa aattgaaact caagcgagcc cttgggcgga ttcaacgatt
     6541 tcaagcaaag attcaaggtg ttcccgctga acggaatcag gctcaaacgt ttttgcctgc
     6601 gcttgaagga aactgcgaac ctgctcaatc gaagtcggct cttgacgggt aatccgctgg
     6661 agcagccagg caagtacgaa ggaatcggta agcgaccttt cttccaagtc ttgaacggcg
     6721 acttccagca tgatcaggcg tttttccaaa tcgggaaact ctttcatttc agacggcctt
     6781 taaaggttgt ttaaaactca aggatattaa aaatgaaaca aattaatcaa gcattacaac
     6841 aaaaactggt tgaatttaaa gaaaaatcgg gtatgaacca aacccaactg gcacgcggta
     6901 tcgatacttc gccggcatcc atcagtatgt atctgaacgg cacttatgcg gaaaaaggcg
     6961 gcaattatga aaccatcgag acgaaaatcg aggcgttttt ggagatgcag aaaagtaagg
     7021 cgcgacgcga agaactggtg ttgggttttg tagcgactaa gacgacccgc cgaatcgcgg
     7081 aagtgatgcg caatgcgcac gaaggcggcg aaacagtggt gatctacggt caggcgggat
     7141 tgggaaagac tcaggcggtc aaaaactact gcgagaaaaa cccagcggcc atcttgattg
     7201 aggctaatcc gagctttacg gctttggtct tgatgcgcaa gttggcgacg gcggcgaagg
     7261 tatcggcgat gggcagcctg aatgatttgt ttgagtctgt atctgaccgc ctgcgcgatt
     7321 cgggccgtct gattgtggtc gatgaagcgg aaaacctgcc cttgcgcgcc cttgagatta
     7381 tccgccgact gcatgatgaa acgggctgcg gcttggtgtt gagcggtatg ccccgactgg
     7441 tggccaacct gcgcggtaag catggcgagc tggtgcaact ttatagccgc gtgtctgttg
     7501 cgctgaattt gggcgaatct ttgccggatg acgagctctt tgagattgcg aaagcggctt
     7561 tgcctgatgc ggtcaagcat ctgctccctg atagtgtaca agcgttgatt acggtcatcg
     7621 ggtttaatga aacgctggag ctggtgcgcc tgatgggcgg tacgacttat cctttgcggc
     7681 agggttatac gaaaaacagt caatcccgtg ttgcatactt ggaagagatt atcggcagtg
     7741 aggcggccgg tcggctggtg gaggcaatgg ctccgtgcaa tctgtttata ccccgttgcg
     7801 agacggcctt gtatgagttg cgaaaccgta aaatccgcag tcagtttgac cggcagacgg
     7861 caggcggtac ccctgcttat gaggccgtta acgatttggc cttggcacac cgcctaagcg
     7921 accgccatgt gtggcgaatt ttaaagcagg cggataagga agcggagcag gagaatttgt
     7981 tttagaatgg aatgccatgc agatgtatgg cattttattt tggagaaaaa tatgaaaaag
     8041 ttttattttg tgctgctggc gttgggtttg gcagcgtgtg ggcaagaaca atcgcagaaa
     8101 gctgatgcgg agcagtattt ttttgccaat aaatatcaat ttgcagatga gaaacaggct
     8161 ttttattttg aacgcgccgc ccgtttccgt gtattgcaac aaggccttgg cggggatttt
     8221 gagaggtttt taaaaggaga aatacctaat caagaaaatc ttgcaaagta tcgtgaaaat
     8281 attactcaag cagtcgctta ttatgcggac acgaatggag atgatgaccc ataccgcgtc
     8341 tgcaaacagg ctgcgcaaga tgcagaaatc ctgatgaaga gtatggtaac aagcggtgga
     8401 ggcggtacaa ctgatttaga taaggaaagt tatcaaaatt accgaaaatc aatgcaagaa
     8461 tgccgtaaaa caataacgga agctgaagcc aatttgccga aaaaataaaa taaacgattc
     8521 taaggccgtc tgaacaacag gcggcttttt tgttgcctac tggcactgtt tcgcccgctg
     8581 caaaagccat gccgtttgaa aatgtaagcc tctgaaagtg cattttaatc tgattttgag
     8641 ggaggcttta atgagcaaaa ttatttgtct gactgccgga cacagtaaca ccgacccggg
     8701 cgcagtcaac ggcagcgacc gtgaggcgga cttggcgcag gatatgcgca acattgtggc
     8761 ttcaatcctg cgtaacgatt acggcctgac cgtacgcacc gacggcgaag gcaaaggcaa
     8821 tatgcccttg cgcgatgcgg tcaagctgat tcgcggctcg gatgtggcga ttgagttcca
     8881 caccaatgcg gcggcgaaca aaacggcgac aggcatcgaa gccttgtcca cgccgaaaaa
     8941 taaacgctgg tgtcaggtgc tgggcaaagc cgttgccaag aaaaccggct ggaaactgcg
     9001 cggcgaagac ggctttaagc cggataacgc agggcaacat tcgcgcctgg cttatgcgca
     9061 ggcaggcggc attgtgtttg agccgttttt tatcagtaac gacactgatt tggccttgtt
     9121 taagacgacc aaatggggca tctgccgcgc gattgcggac gcgatcgcga tggaattggg
     9181 agcgacgaag gtatgaaaaa gtctttgatt gctttatgtg ttgcccattg tgcaaagttg
     9241 aaaaacgatt ttggcgtacc accgttacct gaaatcaaaa tcacgccaag ccctgttcgg
     9301 gtaggctctt tgaaacaaca tccgagcctg cgcttgggta aatcaggcgt ggcagccgcg
     9361 aaacgtgcgg cgcgcaaacg caagaatcgt cattaatcat gggacaggtt gcgttttacg
     9421 aaaagatgat tgggctgtgg tcggccaaaa gccgtgaggc aagcgaacag gcagacttgg
     9481 cggcgtttga atttgcggag ggcgaactgg ccaattatca ggaaatgctg aaacggcacc
     9541 tgcaaaccaa aagtgtggaa tagcaatgcg tattttggat atttttaaaa acccagcgac
     9601 aggcaatgtg tcgcactcga aactgtgggc aaacgttgcc tgcgcggcgg ggacggttaa
     9661 gtttgtgatg ctgcccgacc cgtcggcgga gatttgggcg gtgtatttgg gcattgtcgg
     9721 cggctatgcg gtggcgcgtt cgttggtcag cgtcaaacgt caggaggtcg agaatgaatc
     9781 tcgtgaaact gctggcgaat aactggcaac cgattgccat catcgcgctt gtcggcacgc
     9841 tcttggcggt gtcgcaccat caaggctaca agtcggcttt tgcgaagcag caggcggtca
     9901 ttgacaagat ggaaaaagac aaagcgcaag ccctgctgtt gtcggctcaa aactacgccc
     9961 gcgagctgga acaggcgcgt gcggaagcta aaaaatatga agtcaaggcg cacgctgtcg
    10021 gcatggcttt ggcgaaaaaa caggcggaag tcagccgtct gaaaacggaa aataaaaagg
    10081 aaatcgaaaa tgtccttact caagaccgta aaaatgcagg cggcggttgt attgacggct
    10141 ttggccatca cggcttgcag ctctacaagc gcgccctcgg ctacggaaat taaggttgtc
    10201 gaaaaggcgg tcatgccgac accgcctgcc gcattgatgg tcgcgccggt acgcccaaat
    10261 ccgccgaaag acggcaagac ggccacgctg ttggaacacg ccgccgagtt tggcggctat
    10321 gtggcggagt tggaaaatca aaatcaggct tggcgcgact gggcgggcaa tcactcccgc
    10381 aaagtcggaa actgacaaaa aagcccgcgt agggcgcggg ctgagggtga aagcggattt
    10441 tatacctctt ttacaggggt agcggcggta gtgcttttca gcaaatcgac tgcgtgctga
    10501 cagttttgct tgctggtgta gccttcgccc tgagcgatga tttcatggtt ggctgctttc
    10561 aaacgccaac ggtattcgcc ttttgcgtct ttatagattt caaaatacat aaggtttctc
    10621 ctatgaatga gtacacgttt tcttaccgct ttaacggcaa gtcctggtca ttgagcattt
    10681 gggcggacaa ccctgaagaa gccagggcga aatttcgggc tgcacgagaa aatgcgcact
    10741 atgacggcga agttgtagca aaggtttata catttgtaaa tatttcgtgg gttaagaaat
    10801 tgtacaagcg gacaaaatat ttaatgggta tcaaagaatg acctaccgtg aattagttga
    10861 acgtcagttg gctgtgcgcc atgccgattt ggaattgggc ttaagccgcg cacgcgagca
    10921 agagccgttt gtcattcatg tttccgatct gttggataag gcaggcattg agtacgcggt
    10981 acgcatggat aaggattttc agacgacgtt tcaccttgaa tatccaatta cgaactatga
    11041 cacctttaaa cgtgcggttt ggcaaacttt gggggcgtat tactgtgttt gtaatgatgg
    11101 tgatggactg gagattgcca gcaatcgccc tgacggttac gccgtccgta tcgtattcgg
    11161 cgatgtgccg gtttaaaggg gttttaaatg gactttgaat ttggtttcag aaccctgtgg
    11221 ccgattgcga cggcggcatt ttggttttgg gtcaacggca tttcaggccg cctgaaagag
    11281 gcggacaagc gtatcgacga ccttaaagag gagttgcacg cggtcaagct ctcttatcac
    11341 accaaggcgg acgccaaggc agacagcact aatattgcgg cggccttgga gcgaattgaa
    11401 aacaagttag aaaaagtaaa cgaaaaactg gacaggaaag cagacaaatc atgagcgacc
    11461 cgattttgga agccttggcg cgtattgaaa acaagactga tcaaacgctg aaaaatcaga
    11521 aagaaatgca ggcggaaatt gcgcaaatcc gccaagacac gaaacgcacg gccattacat
    11581 tcggcgcact gggcggcggc gtgattacgg tcggctggga attgcttaaa gcgaaaatgg
    11641 gactgtaatt atggctcacc cgcaagaaat ccgtgaaaag ttacgtcggc tctatgtgag
    11701 cggcgagcaa actttggaaa cggcggcctt gatgtgcgaa atcccgcaga ccactgcgcg
    11761 tgcgtggaaa cgtgcggata aggaaaaagg cgacgactgg gataagatgc gcgccgccta
    11821 cactttggcc ggcggcggta ttgaggactt gagccgtgcg atgttggccg gttttatggt
    11881 gcagtacaac agcacgatga cgatgctgca ggattcgagt accgaagatt tgcccccgtc
    11941 cgaccgcgcc aagctgttgg ccagcctggc cgatgcgttt acgaaaaccg tatcggccaa
    12001 tgcgcgtgtg atgccggaaa cgtcaaaact ggcgacggct ttggaattga ttgagttctt
    12061 gatggcgttt gtgcaagaaa aacatcccaa acatttgcct gcctttgtgg aggtattgga
    12121 gccgtttggg gcggaagtgg agaagaagtt tggttaatag ccgtctatca atctcccata
    12181 acgtgatcct cctactgaaa atacttcaac atctgacttt aaacgctctc ccaatattat
    12241 ggtttggcaa cgggaacagg aatatttatg aaaagcacct tcatacccat tgaattgaag
    12301 aatcgatttg acaccctgct cgtaacattg gggacaaata tcatgaattg gctcgctgtt
    12361 cgccatctct gttttcaatc gatagacgaa agaaccagga gcaagttcat acaattggta
    12421 gcgttgcttc tctgtagacc aattttttac tgccaccagt tctttttcca actcatctat
    12481 acggctaatt gccgccgtat aagaagattg ggcttcaaat aaatcagttt gaataccagc
    12541 aatgatttga ttaagttcga tggttttttg cgctacctct gcctctgttt tgagctggtt
    12601 aaaccctttt aaaagtgatc ctgcttcttt taggcctata aatgcgttag aaatgaatcc
    12661 agtcattata aatactcatg aaaacaaaag aattcctcaa atcccttgcc gaactggccg
    12721 ccagtttgcg ccaagtcatc gaagcggaag tggacggctt tgatgcgtcg cccaaggcta
    12781 ttgctgcacg ccgtgccaag gtgtttgacc cggtaggcgg ttacgagtat ttcgtcaaca
    12841 cctactttcc gcattatatc cgctcccctg aaaaatccga actgcatgcg tttttattca
    12901 gccgtctgcc ggagattatc cgctccccca aaggggaaaa tgaggcggtg ggtgcgccgc
    12961 gtggcgaggg taagtcgacg caggttactc agttgtttac gctgtggtgt attgtgacag
    13021 gccaaaaaca ttatgccgtt attgtgatgg acagcataga tcaggcatac ccgatgctcg
    13081 aagccatcaa ggcggaattg gaatttaatc cgcgcttgaa aaccgacttt ccggaagtat
    13141 gcgggcaggg ccgcgtatgg caggccggta cgattgtgac ggccaatgac gttaaagtcc
    13201 aagtggccgg tagcggtaaa aagctgcgtg gtttgcgtca cggcccttac cgtcctgacc
    13261 tgaccatatt ggacgatatt gagaatgacg agcaagtccg caaccccgaa cagcgcgaca
    13321 agctcaatgc gtggctgact aagactgtat tgcctttggg cggtgtcggc cagaaatacg
    13381 atgtgatata tatcggcacg attttgcatt acgacagcgt acttaaccgc actttgaata
    13441 acccgttttg gcacggtatt aagtttaagg cgatgaaacg ctggcccgac cgcatggatt
    13501 tgtgggacag atgggaggaa cttttccgaa acgacggcga gactgtggcc gaggcgtttt
    13561 atctcgccaa taaagacgag atggagcgcg gtgcgcaaac aagctgggcg gctcgcggcg
    13621 tactcgcgct gatgaaaatc cgtgcgcgtg acggtcatgc gacatttgac agcgaatatc
    13681 aaaacgaccc ggtcagcggc gaagatgcgc cgtttgccaa gtcgatgaag ttttggaacg
    13741 acctgccgtc cgatttggtg tatttcggtg cgctcgaccc gtcgctgggt aaagcggggg
    13801 cgagccgtga cccgtcggcg attatcattg gcggttatca acgtgaaacc ggcaaactgt
    13861 atgtcgtgga agctcagatt aaaaaacgtc tccctgattt gattattgag gacgttatca
    13921 gattgcaccg tcaatatcgt tgcaaactgt ggtttgttga gacggttcag tttcaggaat
    13981 ttttgaaaga tgagctggtc aagcgcagcg cggcgcgtgg aatacctgtc ccggcgcggg
    14041 cggtcaagcc ggtatcggac aagctcttgc ggattgagac tttacagcct cacatggcga
    14101 acggtttgat tctgttgaat gagagccaac agacgctgat acagcagttc cgccattttc
    14161 caaaggctga tcatgatgat ggtcctgatg cagtgcatat gctctggtcg ggggcggtgg
    14221 ccaattgtgt gccgatagaa tggcaaagcc ctaccgataa cgattttgat gacgagataa
    14281 aaagtaaatg gagccgataa tggcaaaaaa gaacaataaa actaaaatcc aaaagcccga
    14341 agctgcattg cagacggacg tggctcaaat tacggcgacc ggtcgggtta tcgccgagca
    14401 tccgtccaat tttattacac cgcaaaagat gcgggccctc ttcgaggatg cggaaagcgg
    14461 cgacatccgc gcccaacacg agcttttcgc ggacattgag gagcgcgaca gcgacatcgc
    14521 ggcaaatatg gggacgcgca aacgcgcgct gctgacgctc aactggcgcg tcgccccgcc
    14581 gcgaaatgcg acgcccgaag aagaaaagct gtccgaccaa gcctacgaaa tgatggacag
    14641 cctgcctacc ctcgaagacc tgattatgga tttgatggac gcggtagggc acggattttc
    14701 tgcgttggag gtcgagtggg tattttcaga cggcctttac ctaccccgaa actttatcca
    14761 ccgcccgcaa agctggttca aatgggacaa agacaacggg ctgctgctgc gtacccgcga
    14821 aaatccggaa ggcgaagcgt tgtggccgct gggctgggtc gttcataccc aaaaatcgcg
    14881 cagcgtccag caggcgcgca acgggctttt ccgcacgctt tcctggctgt atatgttcaa
    14941 acactacgcc gtccacgatt ttgccgagtt tttggagctg tacggcatgc ccatccgtat
    15001 cggcaaatac ggcgcgggcg caaccaaaga ggaaaaaaac accctgcttc gagcggtggc
    15061 ggaaatcggt cacaacgcgg caggcatcat gccagaaggt atggaaatcg agctgcacaa
    15121 cgctgcaagc ggtacgatgg caaccagcaa tccgtttttg cagatggccg actggtgcga
    15181 aaaatcggcg gcgcggctga ttttggggca aacgctaacc agcggtgcgg acggaaaatc
    15241 cagcaccaac gcgctgggca atatccacaa cgaggtacgc cgcgatttgc tggtgtcgga
    15301 cgcaaaacag gtggcgcaaa ccatcacaag ccaaatcatc ggaccgttcc tgcaaatcaa
    15361 ctatcccaat gccgacccaa accgcgtgcc gaaatttgaa tttgacacgc gcgagccgaa
    15421 agacatcgcg gtctttgccg acgctatccc gaaactggtg gatgtcggcg tacaaatccc
    15481 cgaaagctgg gtgcgcgaca aactggtcat tccagatgtg caggagggtg aggctgtgtt
    15541 ggtgcggcag gtaccggaca atccgataaa cagaactgca ttggcggctt tatccgccca
    15601 caccgtacca tctaaggcta cgggcaggca tcaggaaata ttggacggcg cgttggatga
    15661 cgcgctggtt gagcccgatt tcaattctca gctcaacccg atggtgcgtc aggcggttgc
    15721 cgcacttaat gcttgcaaca gctacgagga ggcagatgcc gcactgaatg cgctttatcc
    15781 gaatttggac aacgcgaaac tgcgtaccta tatgcagcag gccttgttta tcagcgatat
    15841 tttgggacaa gaccatgccc gcgcctgatt tgggatttgc cttaagtctg ccgccaaaaa
    15901 aggcaatcga gtggctggaa agtaaaaagg ttacggcgga gagctaccgc aatctgacag
    15961 cctccgaaat tgccaaagtc tatacgattg cccgcatgac cgacttggat atgctcaacg
    16021 acatcaaaac ttcgatggtt gaatcggcaa aaagtggaca gtcgtttgac gattggcgaa
    16081 aaggtatctt gaatctgctc ggcaacaagg gctggctgca tccgaacggg cataacggta
    16141 aggatatcat cgacccagcc accggcgagg tattcggttc gccgcggagg ttggagacga
    16201 tttaccgtac caacatgcaa actgcctaca acgccggtca atatcaagga tatatggcaa
    16261 atattgatgc acggccttat tggatgtatg acgcggtagg cgacagccgc acccgtccgg
    16321 cgcattcggc aatagacggg ctggtgtacc gctacgacga cccgttttgg gcaacgtttt
    16381 acccgcccaa cggctacaac tgccgttgct cggtcatcgc gctgtcggag cgggatgtgg
    16441 aacgccaggg gcggattgtc gggcaaagca cggcggacaa tctggtcgag acccataaaa
    16501 tctacaacaa aaaaggcgat acttatctga cccttgccta taaagcaccg gatggcagtc
    16561 tgtacacgac cgatcgagga tttgattaca acgccggacg aatgaactac cgccccgatt
    16621 tagacaagta cgaccgtgcg ttggcgcatc aatttgccaa agcggaaatg ggtggtgcgg
    16681 attttaaaac cagctttaaa cagcttgaaa aagagtttta tgaagtcaag caacgtttgg
    16741 atattgatgg caagcccgat aaagagcaga aaatcaaaat ccgaaatgcg ctatcaagac
    16801 agcttaaatt tgctgcgggt gtattgagca aggaaacgca agaattggca ggtatgacac
    16861 gagcgacggt gtggctgtct gatgatacat tggtcaaaca ggtggacagt cgggaggggc
    16921 aatcgtttgg aacagattta tatgccatgc tccccgatct gattcatgag cccgaatata
    16981 ttttttatga caaaagcaga ggaaaagaaa gttttgtttt gctcggaaaa ccgttggagg
    17041 ggaacgaaaa atacagattg atggcagttt tgaaatatct gccacagtac gattcgattt
    17101 ttttggattc gtttcgcagg gcggatgaaa aagaaattgt gaaaagtaag aaacgctatg
    17161 aaatgaaaaa aggctactaa gtcggactgc aatcccgaca cactctcgct tatgtaacgg
    17221 ataccaatcc cagtccgagg actgcataag ggctgcggag ggcagacaaa ttcaccgctt
    17281 ttttagtagc ctaggcagga taataccatg attgatgtca aaatagacaa tatctttgtc
    17341 gtcctaaacc aaatcgagcg gcttggcaac gggatcgaaa accgctacct gctgatgcgc
    17401 cgactgtccg aaaccatgca cacggcggtc aagctcaatt tccgctacgc aggccgtccg
    17461 aaatggttgg ggctaaaata ccgcgacggc aagccgcttt cggattcggg tcgtctgaaa
    17521 gacagttttt ccacactgtc agacaacgat acagcccttg tcggtacgaa tatcgtctat
    17581 gccgccatcc acaacttcgg cggtatggcg gggcgcaacc gcaaagttcg gattccgcaa
    17641 cgggaatttt tgacgctgac ggacgacgac aaacaggctt tgatggacga tgtgcaggat
    17701 tatttttcgg gtctgatacc gtgaatttat aaaaccctca aaaacgcgct ttttagcgcg
    17761 tttttttatg cgggtaatac aaacccctgc ccaagatata aaaatcaatc ctagactctt
    17821 ctaaaaagcc cctgaaaacg attaattgtg tatcgcgcgg acaggtttta aaaaaatggc
    17881 gggagggttt gaagcacgcc tactctttgt tgttttttca aataggcaaa atgaccgtat
    17941 tgagagaggt acacatgtcc aaaaatgcac aaaaaaccct acttgccgtg tgcagtttcg
    18001 aggtgcagcc aaaagacggg cgaatccaac tgctgccata tggcgaattt cgcgcagtag
    18061 acggtcgtcc gactgatgtc cctgcgtggt atctgaccga agaaaacggt catgatgtcg
    18121 cgttgttggc caacagctcg cgcaatcagt tggttgtcga ttatgaacac cagacgctct
    18181 acaaagagaa aaacggacaa cctgcacctg ccgccggttg gatgcgttgg ctggagttca
    18241 cgcctaaagg catgtttgcc gaagtggagt ggacggacaa ggcggctgcg gcaattgccg
    18301 caaaagagta tcgctacatc tctgctgtgt tttcctatga cacaaaggga tatgtaagca
    18361 aaatttttca cgccgcgctg acaaatttcc ccgcgttgga cggtatggac gaagtgctgg
    18421 cggcagcgtc ggcgcaaatt ttaaaaccgg aaacggagca aaaccctatg aaagagttgt
    18481 tacagcaact gttcggcctg cctgatgcgg gcgaagaaga actgaaggcg gcattgtccg
    18541 cgctcgtgga agccaagccg gaaggtgcgg ctttgtccgc cgatgtattc gcgcagctgg
    18601 cggaaaaaga cagccgcatt gcggctttgt cggcgcaaaa cggcacgccc gacctgacgc
    18661 gatacgcgcc ggtgtcgctg gtgcgcgacc tgcaaaacca agttgccgcc ctgaccgccg
    18721 agcgcgaagc cgacaaaggc ggcgaactga ttaccgccgc actgtctgct ggcaagctgc
    18781 tgcccgcaca aaaagaatgg gcggaaggcg tgctgaaaca gccgggtggc tttgcctttt
    18841 tgagcggctt tatcgaaaac gcccagccgg tcgctgcact ggcaggctcg caaacgggcg
    18901 gcaaagcacc cgacgaacgc gtcgccgcac tgactgcgga agaggcagcc gcagcaaaaa
    18961 tgctgggcat gtccggcgaa gaatttgtaa aaatcaaaga aagcgaaggt aagtaatgga
    19021 caaatcagcg attttgaccg caatcacggc agcattccgc aaagaatttc aaacgggggc
    19081 ctgcaaaacc gcctgaaccg tttaaccgcc aaataggtgc agccggtagc gcaggcagtg
    19141 cgcctgttat ccacgtcatc gctgttgtcg gtggcaacgg cattaatcga ggcgcatggc
    19201 gaagagatga ccgcgcccga tttgattgag gttaaccgcg ccatgcgccg ccgtatgcag
    19261 gccgagattg ccgccttgcg ggcggtgcag acggctgctg ccgagtctgg tgggctgacg
    19321 gccaacgccg tgtataccga ggcttaccaa acggcagaat ccctgcgcgc ggcggcaggc
    19381 agtctgaatg cgttggttgc ggcggccatc aaccaaaagc cgccgctgat tgtgcgccaa
    19441 gccccaatcg acggtacgat acaccaaatc gcccacgagt tttacggcga tatagcccgc
    19501 gcagcagagc tggtgcgact caatccccat atccaccacc ccgcgtttat caagcgcggc
    19561 actttggtca acagctatgc aaaataattc atacggctat gccgtgtcgg tgcgcgtggg
    19621 cggtaaagag caccgccact gggagcgcta cgacatcgac agcgactttt taatccctgc
    19681 cgacagcttc gattttgtca tcggcaggtt ggggccggag gcggccatac ccgatttaag
    19741 cggagagagc tgcgaggtag tgatagacgg gcaaatcgtg atgacgggca tcatcggcag
    19801 ccagcgccac ggcaaaagca agggcagccg cgagttgagc ttgagcgggc gtgatttggc
    19861 cggttttttg gtggattgct ccgcgccgca gctcaatgta aagggcatga cggtattgga
    19921 tgcagccaaa aagctggccg cgccgtggcc gcagattaaa gcggtggtgc ttaaggccga
    19981 aaacaacccc gctttggaca aaatcgacat cgagccgggc gaaactgtat ggcaggcatt
    20041 aacccatatt gccaactcgg tcgggctgca tccgtggctg gagccggacg gcacgttggt
    20101 ggtgggcggt gcggattaca gcagcccgcc ggtggcgaca ttgtgttgga gccgcaccga
    20161 cagccgctgc aatatcgagc gcatggacat tgagtgggat accgacaacc gcttttccga
    20221 ggttactttt ttggcgcaat cgcacggccg cagcggcgac agcgccaaac acgatttaaa
    20281 gtgggtgtac aaagacccga cgatgacgct gcaccgccct aaaacggtgg tggtgtccga
    20341 tgccgacaat ttggccgcat tgcaaaagca ggctaaaaag cagctggccg actggcggct
    20401 ggagggattt acactcacga taaccgtggg cggccataaa acccgcgacg gcgtattgtg
    20461 gcaacctggc cagcgtgtgc atgtgatcga cgacgagcac ggtatcgatg cggtgttttt
    20521 tctgatgggg cggcggttta tgctatcccg catggatggc acgcaaaccg agctgcggct
    20581 caaagaggac ggtatttgga cacccgacgc ttaccccaaa aaggccgagg cggcgcgcaa
    20641 gcgcaaaggc aaacgcaaag gcgtgagcca taagggcaaa aaaggcggca aaaaacaagc
    20701 agaaacggcg gtgtttgaat gagtttgagt aaattggcga aaaaaacggc acaaactgct
    20761 aaaaatatcg gcgaaaccct gcgcacggcc tttcggggaa aaatcacgct ggtggtgtcg
    20821 tccgagccga tacagcgcgt gcagttgagc ggcttggccg acgaaaccct gcaagacctt
    20881 gaacatttgc aggaatacgg ctttgccagc catccgcccg acggcagcga agcggtagtg
    20941 ataccgctgg gcggcaatac ttcgcacggt gtgattgtgt gcagccagca cggcagctac
    21001 cgcatcaaaa accttaagcc cggcgagacg gcgattttta atcatgaggg tgcaaaaatc
    21061 gtgattaagc aaggcaaaat cattgaggcc gattgcgacg tgtaccgggt taactgcaaa
    21121 caatacgagg ttaatgcggc cacggatgcc aaatttaacg ctccgttggt ggagaccagt
    21181 gcagtgttga cggcgcaagg ccaaatcaac ggcaacggcg gcatggccgt cgagggcggc
    21241 gacggagcca cctttagcgg cgatgttaac caaacgggcg gcagctttaa caccgacggc
    21301 gacgtggtgg ccggcaatat atcgttgcgc cagcacccgc ataccgacag catcggcggc
    21361 aaaaccttac cggcggaacc ggcatagaca agcagacctt tggcagcctt cgggctgctt
    21421 tttttgtgcg tgtgggattg aagcccgtgt actccgtgag gccgtctgaa aacggcaaaa
    21481 tgccaacatg gacaaagagc taaaccccag catcggcgac tataccggcc gcaccgtcga
    21541 tacgctgcaa aatgccgtgt atatccgctt gatgacaccg ttgggcagct ggtgggcgga
    21601 taaaacgctc ggctcgctgc tgcatttgtt gcagcgcgaa aaagacctgc aacgggtcag
    21661 cctgttggcc gagcaatatg ccgatgaggc actgcaaccg attgttaaga gcgggcgtgc
    21721 cgacaagatt accgtgcgcg cagagcagcc gcacgacggc cgcctgatcc tgcatatccg
    21781 ggtggatacg gcggcgggcg ggtttgatta ccgccacgaa gtgcccgtga tttaaagagg
    21841 ttttaaacgt gtttgaaacg ccgacatttg agcaaatccg cgagcgtatc ctgcgcgata
    21901 ccaaaagcct gtggccggat gccgatatca gccccgacag cgaccattat gtgcacgcca
    21961 gccgtttggc cagctgcgcc gaagggcaat atgcgcatca aagctggatt gtgcggcaga
    22021 ttttccctga taccgccgac cgcgagtatt tggagcggca tgcctccatg cgcggcttga
    22081 gccgccgcaa tcctaccacg gccagcggca cgctgaccgt aagcggtatt gcgcaatcca
    22141 tgctttcaga cgacctgcaa gtgcgtatcg gccagcgttt ttaccgcact accgcccgcg
    22201 ccgttatcgg cagcggcggc acggcggaaa taccggcaat cgccgacgag ccgggcgcgg
    22261 ccgccaatgt gcgcgacggc gaggcgcaac tgatggccgc ccccgccggt gtggccaccg
    22321 aatgccgcct taccgtacaa ggcggcaccg accgagaaag cgatgcctca ctgctggcgc
    22381 gtctgttgga aatcatccgc cgaccgcccg caggcggcaa ccgttacgac tataaaaact
    22441 gggcgttgag tgttgacggc gtaaccagcg catatgttta tccgctgcgc cgcggcttgg
    22501 gtacggtgga tattgccatt acctccgccg acggtgtgcc atcggaagaa actgtgcgcc
    22561 gcgtacaggc ttatatcgac gagatgcgcc cggtaacggc aaaaaatgcg ctggtactca
    22621 agccaaccgt aacggcggtg cctgttaccg tgcaagtcaa gctcgacggc atcgacttgg
    22681 acgaggccaa gcgccgcata cggacggccc taaaagaata tttcgacacc ctgatccccg
    22741 gcgacggcct gactgtgtcg caaatcgagg cggctatcag caatgtggat ggtgtgatcg
    22801 accgccgtct gactgcgccg acggccaacc gtgccgccga tacggttaac cgcatcgagt
    22861 ggtttaaagc gggcgcgatt aatgtaacgg agatgccgtc atgagctatc aagacatctt
    22921 gcggggcctg ttgccccccg tgtcgtatgc ccgcaatgcc ccgcgtgtgc gggcgcaggc
    22981 agaaatagac ggcgcagcgc tggatgcggt ggcggaatcg gctcaaagcg ttgccgatgc
    23041 cgtcgacccg cgcagcgccg gccaaatgct ggccgattgg gagcgcgtat taggtttgga
    23101 cggtacgggc aaaaaccgcc agcgccgtgt gttggccgtc atggccaagc taaacgaaac
    23161 aggcggcttg agtattcctt attttgtgcg tttggccgag gcggcgggct atcaaatcca
    23221 aatcgacgaa ccgcagccgt tccgcgccgg tgtaaaccgc gccggcgacc gtcttgcgcc
    23281 gcaggaaatc atgtgggtgt ggcacgttaa cgtgcgcggc ggcaacaacc gcattacccg
    23341 attccgcgcc ggtatctcgg cggcgggcga caggctgacc gattacagcg atgccgtgat
    23401 cgagagcctg ttcaaccgcc tcaagcccgc ccacaccgct atccgattta cctaccgata
    23461 aggactaaac catgcacccc atcgaaaccc ccgacaagac cttccacgac ggcgacggcg
    23521 tgtccgaatt gggcaccatc ctgcccgcgt ggtggctcaa ccaagtgcaa tccgagctgc
    23581 tggccgtgct gactgcggcc ggtatccagc cggataagtc gcagcctaat caattactgg
    23641 cagcactaaa taggctggct gtagttacca ccggcgacca aaccatcacc ggccaaaaaa
    23701 cctttactgc ccaaacccaa ttccaaagcg gcatccattt atccgccaac cagacgaact
    23761 ggaacggcgg ccacaaagcc tacatcggcg cggatgccga caacgcccac atcgtcttcg
    23821 gcgacgacac cctccgcctg cacagcgcaa acaaccgcat ttcctacaac aaccacgaca
    23881 tcttccacaa agccaacaaa ccgcgttttg ccgaagacat cgaaggcaaa ccgaacacac
    23941 tgtccggata cggcatcggc aatttcaaag tcgaaacatt ccggggcgat ttgaacaccc
    24001 tcaaaacaga cggcatctat tccctgccga cggcggtcgg cagctccaac ctgcccgttg
    24061 aaaacaccgc ctgccatatc caagtcatcg ccggcacgaa acacggctgg tgcaggcagt
    24121 tgggttatcc cgcctacacg tccgacgtgt acgaacgcca ccaaacgagc agcgcaaacg
    24181 acaactggtc cgcatggaaa aaactcaatt cggacggcat ccccgtcggc gcgatcgtat
    24241 cctttcccaa agccgtacaa aaccccgcag gctatctcaa agccaacggc acgacctttg
    24301 cgcaaaacac cttccccgac ctctaccgcg ccttgggcaa cagcaaccgc ctgcccgatt
    24361 taagccgtac cgacatcggc atcaccgcgt ggtttccgtc cgaccaaatc ccgaccggct
    24421 ggctggcgtt tgacgacatc cgcacgcgcg taaccgaaac cgcttacccc gagctgtacc
    24481 gtctgctgac cggaaaatac ggcagcatcc aaaacgtccc gcaggcggaa gaccgcttta
    24541 tccgcaacgc gggcaacggc ttagcagtcg gaacgaagca ggaagacgaa atcaaacggc
    24601 acgtccacaa agtattttca cactggacaa accacacaga cgcagccgcc gtcggttacg
    24661 aagaccacaa cgaaaggcag agaagcgcgc tcgtatcgac ttggacggac ggcgatttaa
    24721 acgacaacgg ctttttaacc ccgcgcctgg acagcaaaat ggcgacaggc ggcgcagaaa
    24781 accgccccaa agccctggtt ttaaaactgt gcatcaaagc cgccgacacc ttgggcgaag
    24841 ccgtgttttg gataaagtcc cacggcgaaa ccatcaacgc cggcgcgctg gacgcgggca
    24901 cgctggcgca aaacctgcaa gacaaagccg accgcgccca cacccacacc gccgcccaaa
    24961 tccaagggct ggacgaaaaa atcagcaccg ccgttgccgc gcaattcaca cgccaaacca
    25021 tcggcggcgt ggatattgtc agattccccg acggcacaat gatacagacc ggcagttaca
    25081 ggtttacaag aagcggcggc cccatcgaaa acgaagtcgt cttccccgtc gcctttgccg
    25141 acggcaacgt caaatgcttc gtatccgaac gccattcggg acgcgtcacc ggcgatcgaa
    25201 ggcaacacaa ctggctgttt atccgcgcaa aaaaccacgc cgccgccatt attaccaact
    25261 ggtacgaagg cagttgcgac tggatggcca tcggcaaagc cgcctcggga aacgccgcca
    25321 gctccccgat aggccccgaa atacctgaaa ccaacgaaga accgcaaaga gagagtggaa
    25381 gaacatcaac cggaccccga aaccgccgcc gccaccgaga cggcctgctc gaggcactgc
    25441 aagactagcg ggctgtagag atgggttgta gagatggctg tagagatggc tgtagagatg
    25501 gctgtagaga tgggcttcag cccgccgatc caagcaatcc gaccgaaacc ggccgcgccg
    25561 ccaatccccc gaaacctatg ccccgccaat cctgccactc ttcgtcattc ccgccgcttt
    25621 cgtcattccc gcgaaagcgg gaatccagcc cctaaacgcg gcaggaatct atcggaaaa
//
LOCUS       DAWHSK010000007        23571 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.007, whole genome shotgun sequence.
ACCESSION   DAWHSK010000007 DAWHSK010000000
VERSION     DAWHSK010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 23571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23571
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.007"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            264..908
                     /gene="rplC"
                     /locus_tag="WIC03_000198"
     CDS             264..908
                     /gene="rplC"
                     /locus_tag="WIC03_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEZ3882761.1"
                     /translation="MTLGLVGRKVGMTRVFDEQGVSVPVTVLDMSANRVTQVKSKDTD
                     GYTAVQVTFGQKKANRVNKAEAGHFAKAGVEAGRGLIEFALTEEKLAELKAGDEITVS
                     MFEVGQLVDVTGTSKGKGFSGTIKRHNFGAQRTSHGNSRSHRVPGSIGMAQDPGRVFP
                     GKRMAGQYGNTKATVQKLEVVRVDAERQLLLVKGAVPGAVNSDVVVRPSVKVGA"
     gene            908..1528
                     /gene="rplD"
                     /locus_tag="WIC03_000199"
     CDS             908..1528
                     /gene="rplD"
                     /locus_tag="WIC03_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEZ3882762.1"
                     /translation="MELKVIDAKGQVSGSLSVSDALFAREYNEALVHQLVNAYLANAR
                     SGNRAQKTRAEVKHSTKKPWRQKGTGRARSGMTSSPLWRKGGRAFPNKPDENFTQKVN
                     RKMYRAGMATILSQLARDERLFAIEALTAETPKTKVFAEQVKNLGLEQVLFVTKQLDE
                     NVYLASRNLPNVLVLEAQQVDPYSLLRYKKVIITKDAVAQLEEQWV"
     gene            1525..1839
                     /gene="rplW"
                     /locus_tag="WIC03_000200"
     CDS             1525..1839
                     /gene="rplW"
                     /locus_tag="WIC03_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEZ3882763.1"
                     /translation="MNQQRLTQVILAPIVSEKSNVLAEKRNQMTFKVLANATKPEIKA
                     AVELLFGVQVASVTTVTTKGKTKRFGRTLGRRSDVKKAYVSLAAGQELDLEAAAAAAD
                     KE"
     gene            1845..2678
                     /gene="rplB"
                     /locus_tag="WIC03_000201"
     CDS             1845..2678
                     /gene="rplB"
                     /locus_tag="WIC03_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEZ3882764.1"
                     /translation="MAIVKMKPTSAGRRGMVRVVTEGLHKGAPYAPLLEKKNSTAGRN
                     NNGHITTRHKGGGHKHHYRVVDFKRNKDGIPAKVERIEYDPNRTAFIALLCYADGERR
                     YIIAPRGIQVGAVLVSGAEAAIKVGNTLPIRNIPVGTTIHCIEMKPGKGAQIARSAGA
                     SAVLLAKEGAYAQVRLRSGEVRKINVDCRATIGEVGNEEQSLKKIGKAGANRWRGIRP
                     TVRGVVMNPVDHPHGGGEGRTGEAREPVSPWGTPAKGYRTRNNKRTDNMIVRRRYSNK
                     G"
     gene            2685..2963
                     /gene="rpsS"
                     /locus_tag="WIC03_000202"
     CDS             2685..2963
                     /gene="rpsS"
                     /locus_tag="WIC03_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEZ3882765.1"
                     /translation="MARSLKKGPYVDLHLLKKVDAARASNDKRPIKTWSRRSTILPDF
                     IGLTIAVHNGRTHVPVFISDNMVGHKLGEFSLTRTFKGHLADKKAKKK"
     gene            2972..3301
                     /gene="rplV"
                     /locus_tag="WIC03_000203"
     CDS             2972..3301
                     /gene="rplV"
                     /locus_tag="WIC03_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEZ3882766.1"
                     /translation="MRVNAQHKNARISAQKARLVADLIRGKDVAQALNILAFSPKKGA
                     ELIKKVLESAIANAEHNNGADIDELKVVTIFVDKGPSLKRFQARAKGRGNRIEKQTCH
                     INVTVGN"
     gene            3311..4003
                     /gene="rpsC"
                     /locus_tag="WIC03_000204"
     CDS             3311..4003
                     /gene="rpsC"
                     /locus_tag="WIC03_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEZ3882767.1"
                     /translation="MGQKINPTGFRLAVTKDWASKWFAKSTDFSTVLKQDIDVRNYLR
                     QKLANASVGRVVIERPAKSARITIHSARPGVVIGKKGEDIEVLKRDLQVLMGVPVHVN
                     IEEIRRPELDAQIIADGIAQQLEKRVQFRRAMKRAMQNAMRSGAKGIKIMTSGRLNGA
                     DIARSEWYREGRVPLHTLRANVDYATSEAHTTYGVLGLKVWVYTEGNIKSSKPEHESK
                     QRKAGRRNAAAN"
     gene            3987..4403
                     /gene="rplP"
                     /locus_tag="WIC03_000205"
     CDS             3987..4403
                     /gene="rplP"
                     /locus_tag="WIC03_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEZ3882768.1"
                     /translation="MLQPTRLKYRKQQKGRNTGIATRGNKVSFGEFGLKAVGRGRLTA
                     RQIEAARRAMTRHIKRGGRIWIRVFPDKPITEKPIQVRMGGGKGNVEYYIAEIKPGKV
                     LYEMDGVPEELAREAFELAAAKLPIPTTFVVRQVGQ"
     gene            4403..4594
                     /gene="rpmC"
                     /locus_tag="WIC03_000206"
     CDS             4403..4594
                     /gene="rpmC"
                     /locus_tag="WIC03_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEZ3882769.1"
                     /translation="MKANELKDKSVEQLNADLLDLLKAQFGLRMQNATGQLGKPSELK
                     RVRRDIARIKTVLTEKGAK"
     gene            4594..4857
                     /gene="rpsQ"
                     /locus_tag="WIC03_000207"
     CDS             4594..4857
                     /gene="rpsQ"
                     /locus_tag="WIC03_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEZ3882770.1"
                     /translation="MSETKNVRTLQGKVVSDKMDKTVTVLVERKVKHPLYGKIIRLST
                     KIHAHDENNQYGIGDVVVISESRPLSKTKSWVVSELVEKARSI"
     gene            5080..5448
                     /gene="rplN"
                     /locus_tag="WIC03_000208"
     CDS             5080..5448
                     /gene="rplN"
                     /locus_tag="WIC03_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEZ3882771.1"
                     /translation="MIQMQTILDVADNSGARRVMCIKVLGGSKRRYASVGDIIKVAVK
                     DAAPRGRVKKGDVYNAVVVRTAKGVRRPDGALIKFDNNAAVLLNNKLEPLGTRIFGPV
                     TRELRTERFMKIVSLAPEVL"
     gene            5460..5783
                     /gene="rplX"
                     /locus_tag="WIC03_000209"
     CDS             5460..5783
                     /gene="rplX"
                     /locus_tag="WIC03_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEZ3882772.1"
                     /translation="MNKIIKGDRVVVIAGKDKGKQGQVVRVLGDKVVVEGVNVVKRHQ
                     KPNPMRGIEGGIITKEMPLDISNIAILNPETNKADRVGIKLIENEGKVKRVRFFKSNG
                     SIIGA"
     gene            5793..6332
                     /gene="rplE"
                     /locus_tag="WIC03_000210"
     CDS             5793..6332
                     /gene="rplE"
                     /locus_tag="WIC03_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEZ3882773.1"
                     /translation="MARLREFYKETVVPELVKQFGYKSVMEVPRIEKITLNMGVGEAV
                     ADKKVMEHAVSDLEKIAGQKPVVTVARKSIAGFKIRDNYPVGCKVTLRRDQMFEFLDR
                     LITIALPRVRDFRGVSGKSFDGRGNYNMGVREQIIFPEIEYDKIDALRGLNITITTTA
                     KTDEEAKALLSLFKFPFKG"
     gene            6335..6640
                     /gene="rpsN"
                     /locus_tag="WIC03_000211"
     CDS             6335..6640
                     /gene="rpsN"
                     /locus_tag="WIC03_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEZ3882774.1"
                     /translation="MAKKALINRDLKRQALAKKYAAKRAAIKAVINDSNATEEERFEA
                     RLRFQSIPRNAAPVRQRRRCALTGRPRGTFRKFGLGRIKIREIAMRGEIPGVVKASW"
     gene            6656..7048
                     /gene="rpsH"
                     /locus_tag="WIC03_000212"
     CDS             6656..7048
                     /gene="rpsH"
                     /locus_tag="WIC03_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEZ3882775.1"
                     /translation="MSMHDPISDMLTRIRNAQRANKAAVAMPSSKLKCAIAKVLKEEG
                     YIEDFAVSSDVKSILEIQLKYYAGRPVIEQIKRVSRPGLRIYKASSEIPSVMNGLGIA
                     IVSTSKGVMTDRKARSQGVGGELLCIVA"
     gene            7062..7595
                     /gene="rplF"
                     /locus_tag="WIC03_000213"
     CDS             7062..7595
                     /gene="rplF"
                     /locus_tag="WIC03_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEZ3882776.1"
                     /translation="MSRVAKNPVTVPAGVEVKFGTEALVIKGKNGELSFPLHSDVAIE
                     FNDGKLTFVANNSSKQANAMSGTARALVSNMVKGVSEGFEKRLQLIGVGYRAQAQGKI
                     LNLSLGFSHPIVYEMPEGVSVQTPSQTEIVLTGSDKQVVGQVAAEIRTFRAPEPYKGK
                     GVRYVGEVVVMKEAKKK"
     gene            7609..7962
                     /gene="rplR"
                     /locus_tag="WIC03_000214"
     CDS             7609..7962
                     /gene="rplR"
                     /locus_tag="WIC03_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEZ3882777.1"
                     /translation="MDKHTTRLRRARKTRARIADLKMVRLCVFRSNNHIYAQVISAEG
                     DKVLAQASTLEAEVRGSLKSGSNVEAAAIVGKRIAEKAKAAGVEKVAFDRSGFQYHGR
                     VKALAEAARENGLSF"
     gene            7981..8499
                     /gene="rpsE"
                     /locus_tag="WIC03_000215"
     CDS             7981..8499
                     /gene="rpsE"
                     /locus_tag="WIC03_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEZ3882778.1"
                     /translation="MAKHEIEERGDGLIEKMVAVNRVTKVVKGGRIMAFSALTVVGDG
                     DGRIGMGKGKSKEVPVAVQKAMDQARRSMIKVPLKNGTIHHEVIGRHGATKVFMQPAK
                     EGSGVKAGGPMRLVFDAMGIHNISAKVHGSTNPYNIVRATLDGLSKLHTPADIAAKRG
                     LTVEDILGVNHG"
     gene            8492..8677
                     /gene="rpmD"
                     /locus_tag="WIC03_000216"
     CDS             8492..8677
                     /gene="rpmD"
                     /locus_tag="WIC03_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEZ3882779.1"
                     /translation="MAEQKKIRVTLVKSLIGTIESHRACARGLGLCRREHTVEVLDTP
                     ENRGMINKISYLLKVES"
     gene            8679..9113
                     /gene="rplO"
                     /locus_tag="WIC03_000217"
     CDS             8679..9113
                     /gene="rplO"
                     /locus_tag="WIC03_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEZ3882780.1"
                     /translation="MFLNTIQPAVGATHAGRRVGRGIGSGLGKTGGRGHKGQKSRSGG
                     FHKVGFEGGQMPLQRRLPKRGFKSLTASANAQLRLSELESIAVNEIDILVLKQAGLIA
                     STVSNVKVIASGEISKAVALKGIKVTKGARAAIEAVGGKIEM"
     gene            9125..10435
                     /gene="secY"
                     /locus_tag="WIC03_000218"
     CDS             9125..10435
                     /gene="secY"
                     /locus_tag="WIC03_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEZ3882781.1"
                     /translation="MANQQTSSGSSKFGDLKKRLLFLFGALIVFRIGAHIPVPGVDAV
                     ALAKLYESAGNGILGILNMFSGGSLERFSIFAIGIMPYISASIIVQLASEILPSLKAL
                     KKEGEAGRKVITKYTRYGTVLLAILQSLGVASFVFQQGIVVTSSFEFHVSTVVSLVTG
                     TMFLMWLGEQITERGIGNGISLIITAGIASGIPSGIAKLVTLTNQGSMSMLTALFIVF
                     GALLLIYLVVYFESAQRKIPIHYAKRQFNGRAGSQNTHMPFKLNMAGVIPPIFASSII
                     LFPSTLLGWFGSADTNSVLHKIAGLLQHGQLLYMALFAATVIFFCYFYTALVFSPKEM
                     AENLKKSGAFVPGIRPGEQTSRYLEKVVLRLTLFGALYITTICLIPEFLTTVLNVPFY
                     LGGTSLLILVVVTMDFSTQINSYRLTQQYDKLMTRSEMKSFSRK"
     gene            10440..10658
                     /gene="infA"
                     /locus_tag="WIC03_000219"
     CDS             10440..10658
                     /gene="infA"
                     /locus_tag="WIC03_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEZ3882782.1"
                     /translation="MAKEDTIQMQGEILETLPNATFKVKLENDHIVLGHISGKMRMHY
                     IRISPGDKVTVELTPYDLTRARIVFRAR"
     gene            10679..10792
                     /gene="rpmJ"
                     /locus_tag="WIC03_000220"
     CDS             10679..10792
                     /gene="rpmJ"
                     /locus_tag="WIC03_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEZ3882783.1"
                     /translation="MRVQPSVKKICRNCKIIRRNRVVRVICTDLRHKQRQG"
     gene            10858..11220
                     /gene="rpsM"
                     /locus_tag="WIC03_000221"
     CDS             10858..11220
                     /gene="rpsM"
                     /locus_tag="WIC03_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEZ3882784.1"
                     /translation="MARIAGVNIPNNAHIVIGLQAIYGIGATRAKLICEAANIAPDTK
                     AKDLDETQLDALRDQVAKYEVEGDLRREVTMSIKRLMDMGCYRGFRHRRGLPCRGQRT
                     RTNARTRKGPRKAIAGKK"
     gene            11240..11635
                     /gene="rpsK"
                     /locus_tag="WIC03_000222"
     CDS             11240..11635
                     /gene="rpsK"
                     /locus_tag="WIC03_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEZ3882785.1"
                     /translation="MAKANTASRVRKKVRKTVSEGIVHVHASFNNTIITITDRQGNAL
                     SWATSGGAGFKGSRKSTPFAAQVAAEAAGKVAQEYGVKNLEVRIKGPGPGRESSVRAL
                     NALGFKITSITDVTPLPHNGCRPPKKRRI"
     gene            11655..12275
                     /gene="rpsD"
                     /locus_tag="WIC03_000223"
     CDS             11655..12275
                     /gene="rpsD"
                     /locus_tag="WIC03_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEZ3882786.1"
                     /translation="MARYIGPKCKLARREGTDLFLKSARRSLDSKCKIDSAPGQHGAK
                     KPRLSDYGLQLREKQKIRRIYGVLERQFRRYFAEADRRKGSTGELLLQLLESRLDNVV
                     YRMGFGSTRAEARQLVSHKAIVVNGQVVNIPSFQVKAGDVVSVREKAKKQVRIQEALG
                     LATQIGLPGWVSVDADKLEGVFKNMPDRSELTGDINEQLVVEFYSK"
     gene            12301..13287
                     /gene="rpoA"
                     /locus_tag="WIC03_000224"
     CDS             12301..13287
                     /gene="rpoA"
                     /locus_tag="WIC03_000224"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEZ3882787.1"
                     /translation="MQNSTTEFLKPRQIDVNTFSATRAKVSMQPFERGFGHTLGNALR
                     RILLSSMNGFAPTEVAIAGVLHEYSTVDGIQEDVVDILLNIKGIVFKLHGRSQVQLVL
                     KKSGSGVVSAGDIELPHDVEILNPGHVICHLADNGQIEMEIKVEQGRGYQSVSGRQVV
                     RDENRQIGAIQLDASFSPISRVSFEVEPARVEQRTDLDKLVLDIETDGSIDPEEAVRS
                     AARILIDQMSIFADLQGTPVEEVEEKAPPIDPVLLRPVDDLELTVRSANCLKAEDIYY
                     IGDLIQRTETELLKTPNLGRKSLNEIKEVLASKGLTLGSKLEAWPPVGLEKP"
     gene            13311..13679
                     /gene="rplQ"
                     /locus_tag="WIC03_000225"
     CDS             13311..13679
                     /gene="rplQ"
                     /locus_tag="WIC03_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEZ3882788.1"
                     /translation="MRHRNGNRKLNRTSSHRAAMLRNMANSLLTHETIVTTLPKAKEL
                     RRVVEPLITLGKKPSLANRRLAFDRTRDRDVVVKLFGDLGPRFTARNGGYVRVLKYGF
                     RKGDNAPLALVELVDKPAAE"
     gene            13826..14539
                     /gene="minC"
                     /locus_tag="WIC03_000226"
     CDS             13826..14539
                     /gene="minC"
                     /locus_tag="WIC03_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HEZ3882789.1"
                     /translation="MMVYIMNAFDIKSTKMDVLSISLHTSDLFDLEDVLVKLGKKFQE
                     SGVVPFVLDVQEFDYPESLDLAALVSLFSRHGMQILGLKHSNERWAAAAMKYHLLFCL
                     SHSENVKELGQVEVQNTEDGQKARKTVLITSPVRTGQQVYAEDGDLIVTGAVSQGAEL
                     IADGNMHIYAPMRGRALAGAKGDTSARIFIHSMQAELVSVAGIYRNFEQDLPDHLHKQ
                     PVQILLQDNRLVISAIGSE"
     gene            14568..15383
                     /gene="minD"
                     /locus_tag="WIC03_000227"
     CDS             14568..15383
                     /gene="minD"
                     /locus_tag="WIC03_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HEZ3882790.1"
                     /translation="MAKIIVVTSGKGGVGKTTTSASIATGLALRGYKTAVIDFDVGLR
                     NLDLIMGCERRVVYDLINVIQGEATLNQALIKDKNCENLFILPASQTRDKDALTREGV
                     EKVMQELSGKKMGFEYIICDSPAGIEQGALMALYFADEAIVTTNPEVSSVRDSDRILG
                     ILQSKSHKAEQGGSVKEHLLITRYSPERVAKGEMLSVQDICDILHIPLLGVIPESQNV
                     LQASNSGEPVIHQDSVAASEAYKDVIARLLGENREMRFLEAEKKSFFKRLFGG"
     gene            15387..15650
                     /gene="minE"
                     /locus_tag="WIC03_000228"
     CDS             15387..15650
                     /gene="minE"
                     /locus_tag="WIC03_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HEZ3882791.1"
                     /translation="MSLIEFLFGRKQKTATVARDRLQIIIAQERAQEGQAPDYLPTLR
                     KELMEVLSKYVNVSLDNIRISQEKQDGMDVLELNITLPEQKKV"
     gene            15654..16574
                     /gene="oxyR"
                     /locus_tag="WIC03_000229"
     CDS             15654..16574
                     /gene="oxyR"
                     /locus_tag="WIC03_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator OxyR"
                     /protein_id="HEZ3882792.1"
                     /translation="MTLTELRYIVAVAQERHFGRAARRCFVSQPTLSIAIKKLEEELA
                     VSLFDRSSNDIITTEAGERIVAQARKVLEEAELIRHLANEEQNELEGAFKLGLIFTVA
                     PYLLPKLIVSLRRTAPKMPLMLEENYTHTLTESLKRGDVDAIIVAEPFQEPGIVTEPL
                     YDEPFFVIVPKGHSFEELDAVSPRMLGEEQVLLLTEGNCMRDQVLSSCSELAAKQRIQ
                     GLTNTLQGSSINTIRHMVASGLAISVLPATALTENDHMLFSIIPFEGTPPSRRVVLAY
                     RRNFVRPKALSAMKAAIMQSQLHGVSFIRD"
     gene            complement(16571..16762)
                     /locus_tag="WIC03_000230"
     CDS             complement(16571..16762)
                     /locus_tag="WIC03_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3882793.1"
                     /translation="MLIHYTWHCIGTDENIHSDAKVEYLQDGSFRGDAILKIDDDGNI
                     LAYRVVGSGKWRFENNACA"
     gene            complement(16904..19741)
                     /locus_tag="WIC03_000231"
     CDS             complement(16904..19741)
                     /locus_tag="WIC03_000231"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEZ3882794.1"
                     /translation="MLDKYSPAEIESKHYQNWESQGYFQPDMDLTKPSFSIQLPPPNV
                     TGTLHMGHAFNQTIMDGLTRYYRMKGCNTAWIPGTDHAGIATQIVVERQLAAQNVSRH
                     NLDREKFLEKVWEWKEVSGGTITQQMRRVGCSADWTREYFTMDDVRAETVTEVFVRLY
                     EQGLIYRGKRLVNWDPVLGTAVSDLEVESVEEQGSMWHIRYPLADNPAEAVIVATTRP
                     ETLLGDVAVAVNPEDERYTHLIGKELILPLTGRTIPVIADEYVEKDFGTGCVKITPAH
                     DFNDYEVGKRHDTRLVNVFDLEAKVLANAEVFNFKGEAQPSFALPEKYAGLDRFAARK
                     QMVADLQEQGFLVEIKAHTLMTPKGDRTGSVIEPMLTSQWFVAMSATPNGGEPDSEFK
                     GLSLADKAKKAVDSGAVRFIPENWVNTYNQWMNNIQDWCISRQLWWGHQIPAWYDEAG
                     NVYVARNQVEAEKQAGKTGLTREEDVLDTWFSSALVPFSTLGWPSETDELKAFLPSNV
                     LVTGYEIIFFWVARMIMMTTHFTGKVPFKDVYIHGIVRDHEGKKMSKSEGNVIDPVDL
                     IDGIGLEKLLVKRTTGLRKPETAPKVEEATKKLFPEGIPSMGADALRFTMASYASLGR
                     SVNFDFKRAEGYRNFCNKIWNATNFVLMNTENQDCGYGATAAEPRGYSFPDMWIVGRL
                     NQTIEQVTQAYETYRFDLAAETLYSFVWNDYCDWYLELAKVQLQTGCASRQRATRHTL
                     LRVLEAALRLLHPIIPFITEELWQTVAPMCDAKTADSIMLARFPEADGGDIVQTAFGQ
                     MTVLQDLIGAVRNLRGEMGIQPNVKAPLFVESADDLADYLKYLPMMTRLTEAQQVATL
                     PESEDAPVAVCNGARLMLKVEIDKATETARLSKEAEKLQKALDKLNAKLSKPGYTEKA
                     PAHLVEKDKADLAELEDKMAKVQTQLSKLKD"
     gene            complement(19838..20647)
                     /gene="zupT"
                     /locus_tag="WIC03_000232"
     CDS             complement(19838..20647)
                     /gene="zupT"
                     /locus_tag="WIC03_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HEZ3882795.1"
                     /translation="MPDFSMSNLAVAFSITLAAGLFTVLGSGLVMFSKTPNPRVLSFG
                     LAFAGGAMVYVSLTEIFSKSSEAFAEIYDKDHAFAAATMAFLAGMGGIALIDRLVPNP
                     HETLDAQDPSFQESKRRHIARVGMMAAFAITAHNFPEGLATFFATLENPAVGMPLALA
                     IAIHNIPEGISIAAPVYFATRSRKKTVWACLLSGLAEPLGAALGYLVLQPFLSPAVFG
                     SVFGVIAGVMVFLALDELLPAAKRYSDGHETVYGLTMGMAVIAVSLVLFHF"
     gene            complement(20828..22084)
                     /locus_tag="WIC03_000233"
     CDS             complement(20828..22084)
                     /locus_tag="WIC03_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HEZ3882796.1"
                     /translation="MKVLVLGAGVAGVSSAWYLAEAGHEVTVIDRAEGVAMETSFANA
                     GQLSYGYTTPWAAPGIPTKALKWLFKSHPPLLFRPDGSLYQIEWLWQMLQNCTAAHYQ
                     TNKERMVRISEYSREMFRRFEAQTGMNFEERKKGTLQIFRQTKEVEAAEQDIAVLERY
                     GVPYRRLKPEECAEFEPALARVTAKIAGGLHLPADATGDCHLFTENLYKLCQEKGVQF
                     HFNQTISRIDHNGLRIKAVETETGRFEADAVVCALGCFSRTVLAQLDLNLPIYPVKGY
                     SLTLPVTNSDGAPVSTVLDESYKVAITRFDNRIRVGGMAELSGYEIKLPEKRRETLAL
                     VVNDLFPEGGDLSQASFWSGLRPMTPDSTPLIGRTRFDNLFLNTGHGTLGWTMSLGSA
                     KLTADIVSGKDTEIRSDDLSLSRYQA"
     gene            complement(22163..23554)
                     /locus_tag="WIC03_000234"
     CDS             complement(22163..23554)
                     /locus_tag="WIC03_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEZ3882797.1"
                     /translation="MQVFLDNLKAFFETVSGWVWGSVMLMLLVGTGIVLTVMLKGLQF
                     TMLGYALKQAFVPSKKYEGGEGHEGDISHFAALMTALSATIGTGNIAGVATAVVTGGP
                     GAVFWMWMTAIFGMATKYGEGVLAVKYRVNNSKGEMSGGPMYYIEKGLGKNWKWMAVA
                     FALFGTFASFGIGSSVQSNSVAQAVQTSFGIEPAYTGITLTVLTAVVVLGGIKGIAKA
                     ASFIVPAMAVFYVLGGLSIIAINSDALMPAVKLIFSDAFSAQAVAGGAIGTVIRYGVA
                     RGVFSNEAGMGSAPIAAAAAKTDHPVRQALVSMTGTFLDTIVVCSITGIVLVMGLLGA
                     GGEFVKPEVSGAALTTVTFQKMLPGIGGWIVTIGLIFFAYSTILGWCYYGEKCAVYVF
                     GEKFAGLYRVGYVSSVMLGTVLSLDLVWLASDTFNGLMALPNLIALLLMAKVIVNETR
                     DFKQKITNGELPH"
BASE COUNT         6123 a         4974 c         6006 g         6468 t
ORIGIN      
        1 catcaaccat attccggttt gtcggagaaa gatgcatacg ctgtgatgac cggataccga
       61 ccctttaaaa gagtccgacc ctatgccgtc tgaaaattca aaacgcttca gacggcatat
      121 tgaagatatt tctgatattt ctgttgatat ttctttaact tgtcagatat aatgccgagc
      181 ttggtacatt tgtgccaagt ttaactttgt ctgaaagaca ggccaatcgc agcctgtccc
      241 tttactttaa aaggaaaata atcatgactt taggtctggt tggacgcaaa gttggtatga
      301 cccgcgtgtt cgacgaacag ggtgtttctg ttccggtaac cgttttggat atgtcagcca
      361 accgcgtcac acaagtaaaa tccaaagata ctgacggcta tactgccgtt caagttacct
      421 ttggtcagaa aaaagccaat cgtgtcaaca aagccgaagc cggacacttt gcaaaagcag
      481 gtgttgaagc cggtcgcggt ttgattgagt ttgctttgac tgaagaaaaa ttagctgaat
      541 tgaaagccgg tgacgaaatc accgtttcta tgtttgaagt tggtcaactg gtcgatgtaa
      601 ccggtacctc taaaggtaaa ggtttctccg gtacgatcaa acgtcataac ttcggcgccc
      661 agcgtacttc ccacggtaac tcccgttctc accgtgttcc aggctctatc ggtatggcgc
      721 aagacccggg tcgcgtgttc cccggtaaac gcatggccgg ccaatacggc aacaccaaag
      781 caactgttca aaaattggaa gttgtccgtg ttgacgcaga acgccaactg ctgttggtta
      841 agggtgctgt tccaggtgcg gtcaacagcg atgttgtagt tcgtcccagc gtgaaagtag
      901 gtgcgtaatg gaattgaaag taattgacgc taaaggacaa gtttcaggca gtctgtctgt
      961 ttctgatgct ttgttcgccc gcgaatacaa tgaagcgttg gttcatcagc tggtaaatgc
     1021 ctacttggca aacgcccgct ctggtaaccg tgctcaaaag acccgtgccg aagtaaaaca
     1081 ctcaaccaaa aaaccatggc gtcaaaaagg taccggtcgc gcccgttccg gtatgacttc
     1141 ttctccgctg tggcgtaaag gcggtcgcgc gttcccgaac aaacccgacg aaaacttcac
     1201 tcaaaaagta aaccgcaaaa tgtaccgtgc cggtatggcg actattctgt cccaattggc
     1261 tcgtgacgag cgtttgtttg cgattgaggc gttgactgcc gaaactccta aaaccaaggt
     1321 ttttgctgaa caagtgaaaa atctaggtct ggagcaagtg ttgtttgtaa ccaaacagct
     1381 cgacgagaat gtttacttgg cttcacgcaa cttgccaaac gtgttggttt tggaagctca
     1441 acaagttgat ccttatagct tgctgcgtta caaaaaagta atcatcacta aagatgcagt
     1501 tgcacaatta gaggagcaat gggtatgaat caacaacgtt tgactcaagt gattttggca
     1561 cctatcgttt ctgaaaaaag caacgtattg gctgaaaaac gcaaccaaat gacgtttaag
     1621 gttttggcaa atgcaaccaa acctgaaatc aaagctgctg ttgagctgct gtttggtgtt
     1681 caagttgctt ctgtaactac cgttacaact aaaggcaaaa ctaagcgttt tggtcgtact
     1741 ttgggccgcc gcagcgatgt taaaaaagct tatgtaagct tggctgccgg tcaagagttg
     1801 gatttggaag ccgctgctgc agctgcagat aaggaataaa caaaatggca atcgttaaaa
     1861 tgaagccgac ctctgcaggc cgtcgcggca tggttcgcgt ggtaacagaa ggtttgcaca
     1921 aaggtgcacc ttatgcgccc ttgcttgaaa agaaaaattc tactgccggc cgtaacaata
     1981 atggtcatat caccacccgt cacaaaggcg gcggtcataa acaccattac cgtgttgtag
     2041 actttaaacg taacaaagac ggcattcctg ccaaagtaga gcgtatcgaa tacgatccta
     2101 accgtactgc cttcatcgca ctgttgtgct atgcagacgg cgagcgtcgt tacatcatcg
     2161 ctcctcgcgg tattcaagtc ggtgccgtat tggtttccgg tgctgaagct gccatcaaag
     2221 taggcaacac cctgccgatc cgcaacattc ccgttggtac gactatccac tgtatcgaaa
     2281 tgaaacccgg taaaggtgct caaatcgcac gttctgccgg tgcttctgcg gtattgctgg
     2341 ctaaagaagg cgcgtacgct caagtccgcc tgcgctctgg cgaagtccgt aaaatcaacg
     2401 tagattgccg tgcaaccatc ggtgaagtcg gtaacgaaga gcaaagcctg aaaaaaatcg
     2461 gtaaagccgg tgccaatcgt tggcgcggta ttcgtccgac tgtacgtggt gttgtcatga
     2521 accctgtcga tcacccgcat ggtggtggtg aaggccgtac gggcgaggcc cgcgaaccgg
     2581 tcagcccatg gggtactcct gctaaaggct accgcactcg taataacaaa cgcacggata
     2641 acatgattgt tcgtcgccgt tactcaaata aaggttaatt tagtatggct cgttcattga
     2701 aaaaaggccc atatgtagac ctgcatttgc tgaaaaaagt agatgctgct cgcgcaagca
     2761 acgacaaacg cccgattaaa acctggtctc gtcgttctac cattctgcct gattttatcg
     2821 gtctgaccat tgctgtgcac aacggccgca cccatgtgcc tgtgtttatc agcgacaata
     2881 tggttggtca taaattaggc gaattctcat tgacccgtac ctttaaaggc cacttggccg
     2941 ataaaaaggc taaaaagaaa taaggtgaat catgagagta aatgcacaac ataaaaatgc
     3001 ccgtatctct gctcaaaagg ctcgtttggt agctgatttg attcgtggta aagacgttgc
     3061 ccaagctttg aatattttgg ctttcagtcc taaaaaaggt gccgagctga ttaaaaaagt
     3121 attggagtca gctattgcta atgccgagca caataacggt gcggacattg atgaactgaa
     3181 agtggtaact atctttgttg acaaaggccc aagcttgaaa cgttttcaag ctcgcgccaa
     3241 aggtcgcggt aaccgcatcg aaaaacaaac ttgtcatatc aatgtgacag tgggtaacta
     3301 aggaaaagct atgggacaaa agattaaccc tacaggcttt cgcctggcgg taactaaaga
     3361 ctgggcttca aaatggtttg ctaaaagcac cgacttttct actgttttga agcaggatat
     3421 cgatgttcgc aattatttgc gtcaaaaatt ggccaatgct tcggttggtc gagtggttat
     3481 tgaacgccct gcaaaatctg cacgcattac cattcactcc gctcgtccgg gtgtggttat
     3541 cggtaaaaaa ggtgaggata tcgaggtttt gaaacgtgac ttgcaagtct tgatgggtgt
     3601 acctgttcat gtaaatattg aagagattcg ccgtcctgag ttggatgctc aaattattgc
     3661 tgacggtatt gcccagcagt tggaaaagcg cgttcaattc cgtcgtgcta tgaaacgagc
     3721 aatgcaaaat gcaatgcgtt ctggtgctaa aggcattaag attatgactt caggccgtct
     3781 gaatggtgcg gatattgccc gtagtgaatg gtatcgtgaa ggtcgcgtgc cactgcatac
     3841 tttacgtgca aatgtagatt atgcaaccag cgaagcgcac accacatatg gtgtattggg
     3901 tctgaaagtt tgggtttata cggaaggcaa tattaaatct tccaaacctg aacatgagag
     3961 taaacaaaga aaggcaggta gacgtaatgc tgcagccaac tagactgaaa taccgtaagc
     4021 aacaaaaggg tcgcaatact ggcatcgcta ctcgcggtaa taaggtaagt ttcggtgagt
     4081 tcggcttgaa agccgtaggt cgtggtcgtt tgactgcccg tcaaatcgaa gctgctcgtc
     4141 gtgcaatgac ccgtcatatc aaacgtggtg gtcgtatttg gattcgtgta ttccctgata
     4201 aaccgattac tgaaaagcct attcaagttc gtatgggtgg cggtaaaggt aacgtggaat
     4261 attacattgc cgaaattaaa ccaggtaaag tgttgtatga aatggatggc gttccagagg
     4321 aactggctcg tgaagcattc gagttggctg ctgccaaatt gcctattcct acaacctttg
     4381 tagtaagaca ggtgggtcaa taatgaaagc aaatgaattg aaagacaaat ccgttgagca
     4441 gttgaatgca gatttgttgg acttgttgaa agctcagttt ggcttacgta tgcaaaacgc
     4501 taccggtcaa ttaggcaaac caagtgaatt gaaacgtgta cgtcgcgata ttgctcgtat
     4561 taaaaccgtt ttaactgaaa aaggtgctaa gtaatgagcg aaactaaaaa tgttcgtact
     4621 ttgcaaggca aagtagtaag cgacaaaatg gataaaaccg taacagtatt ggttgagcgt
     4681 aaagtaaaac atccgctgta tggtaagatt attcggttat ctactaaaat ccatgcccat
     4741 gatgaaaata atcaatatgg aattggtgat gtggttgtta tatcggaatc ccgtccattg
     4801 tcaaaaacta aatcttgggt tgtcagtgag ctggttgaga aagcacgttc tatttaagaa
     4861 ttaaagcaac gtgcttggaa tgggaaacga agtattgcag caaatttaat ttgcgtgtaa
     4921 acttcgtttc ctgtctttca gtttcttctg gaagtttctt cccttttcgg ggtccaagac
     4981 tggtttactt gaaccgcaag gtttcattta ataagcagcg gctttgctgt aagttatctg
     5041 aaagtggtaa attaagttgg ttaatttaaa ggtaataaca tgattcaaat gcagaccatc
     5101 ttagatgtgg ctgataactc tggtgcgcgt cgcgtaatgt gtatcaaggt attgggcgga
     5161 tctaagcgtc gctacgcttc tgttggcgat attattaaag tggcagttaa agatgcggct
     5221 ccgcgtggcc gtgtcaaaaa aggcgatgta tataatgcgg tagttgttcg tactgctaag
     5281 ggtgtacgtc gtcctgatgg tgcgttaatt aaattcgata acaatgccgc cgtgttactg
     5341 aataataaac ttgaaccttt gggtactcgt atctttggtc cggtaacccg tgaattgcgt
     5401 actgagcgat ttatgaaaat cgtttcattg gcacctgaag tattataagg aatggcacga
     5461 tgaataaaat cattaaaggc gatagggttg tagtaattgc tggtaaggat aaaggtaagc
     5521 agggtcaagt agttcgagtg ttgggtgata aagttgttgt tgagggcgtt aatgttgtaa
     5581 aacgccatca aaaacctaat ccaatgcgtg gcattgaggg cggtattatt actaaagaaa
     5641 tgcctttgga tatttctaat atcgcaatcc tgaatccgga aactaataaa gcggaccgtg
     5701 ttggtattaa gctgattgaa aatgaaggca aagttaaacg cgttcgtttc ttcaaatcaa
     5761 atggctctat cattggggca taaggagata acatggctcg gttgagagag ttttataaag
     5821 agacagttgt tcctgaattg gttaaacaat ttggttacaa atcagtaatg gaagtcccgc
     5881 gtattgaaaa aattaccttg aatatgggtg tgggtgaggc tgttgctgat aaaaaagtta
     5941 tggaacatgc tgtttccgat ttagagaaaa ttgccggtca aaaaccggtt gttactgttg
     6001 cccgtaaatc tatcgcaggt tttaaaatcc gtgataacta tccggttggt tgcaaagtaa
     6061 cattgcgtcg tgatcaaatg tttgaattct tggatcgttt gattactatt gcattacctc
     6121 gcgtacgtga cttccgtggt gtgagcggta aatcatttga tggccgtggc aattacaata
     6181 tgggtgttcg tgagcaaatt atttttccgg aaattgaata cgataaaatt gatgctttgc
     6241 gtggtttgaa tattactatt actactacag caaaaaccga tgaggaagcg aaagctttat
     6301 tgtcattgtt taaatttccg ttcaaaggat aatcatggct aagaaagcac ttattaatcg
     6361 tgatctgaaa cgtcaagctt tggctaaaaa atatgcggct aaacgcgcgg caattaaagc
     6421 ggtaatcaat gattcgaatg caactgagga agagcgtttt gaggctcgtt tgaggtttca
     6481 atccattcct cgtaatgcgg cacctgtgcg tcaacgtcgt cgttgtgctt tgacaggtcg
     6541 ccctcgtggt actttccgta aatttggttt gggtcgtatt aaaatccgtg aaatcgccat
     6601 gcgtggcgaa attccgggtg ttgttaaagc cagctggtaa taggagtaat taagaatgag
     6661 tatgcatgat cctatttccg atatgttgac tcgtatccgc aatgcgcaac gtgctaataa
     6721 agcagcggtt gcaatgcctt cttcaaaatt aaagtgtgct attgcaaagg tattgaaaga
     6781 agaaggatat attgaggact tcgcagtttc atctgacgta aagtctatat tggaaattca
     6841 attaaaatac tatgcaggtc gtcctgtaat tgaacaaatc aagcgtgtat ctcgccctgg
     6901 tttgcgtatt tataaagcgt ctagtgagat tccaagtgtt atgaatggct tgggtattgc
     6961 tattgttagt acttctaaag gtgtaatgac tgatcgtaaa gcacgttctc aaggtgttgg
     7021 tggtgagttg ttatgcattg tagcctagtg gaggaaaaga aatgtcacgt gtcgcaaaaa
     7081 acccagtgac tgttcccgct ggtgtagaag taaaatttgg aacagaggca ttagttatta
     7141 agggtaagaa cggtgaattg tcttttcctt tgcattctga tgtagccatt gaatttaatg
     7201 atggcaaatt gacttttgtt gcgaataaca gcagtaaaca agcaaatgca atgtctggta
     7261 ctgctcgcgc attagtcagc aatatggtta aaggtgtttc agaaggtttt gagaaaagat
     7321 tgcaattgat aggtgtgggt tatcgtgctc aagcacaagg taaaatcttg aatctgtctt
     7381 tgggtttttc tcatccgatc gtatatgaaa tgcctgaagg tgtctccgtt caaactccta
     7441 gccaaacaga gattgtttta accggctcgg ataaacaagt tgttggtcaa gttgctgctg
     7501 agattcgtac gttccgtgct cctgagcctt ataaaggtaa aggtgttcgc tatgtaggcg
     7561 aagtagtggt aatgaaagaa gccaagaaaa aataattgag gttcactaat ggataaacat
     7621 acaacccgac tccgtcgtgc acgcaaaacc cgtgctcgta ttgcggactt gaaaatggta
     7681 agattatgtg tgttccgaag caataatcat atttatgctc aagtaattag tgctgaaggt
     7741 gataaagtat tggctcaagc ctctacattg gaagctgagg tgcgcggtag tctgaaatct
     7801 ggaagcaatg ttgaagcagc tgcaatagtt ggtaaacgta ttgctgagaa ggctaaagca
     7861 gcaggtgtag aaaaggttgc ttttgatcgt tcaggtttcc aatatcacgg tcgtgtgaag
     7921 gctttggctg aagctgctcg tgaaaatggt ttaagcttct aaatatttgg agactttcag
     7981 atggcaaaac atgaaattga agaacgcggt gacggtctga ttgaaaagat ggtcgcagtt
     8041 aaccgtgtaa ccaaagtagt taaaggtggt cgcattatgg ctttctctgc gctaactgtt
     8101 gttggtgatg gagatggtcg tattggtatg ggtaaaggta agtcaaaaga agtgcctgtt
     8161 gcagtccaaa aggcgatgga tcaagcacga cgctctatga ttaaggtacc attaaaaaat
     8221 ggtacgattc atcatgaggt tattggtcga catggtgcta ctaaagtatt tatgcagcct
     8281 gctaaagagg gtagtggcgt aaaagccggt ggacctatgc gtttggtttt tgatgctatg
     8341 ggcattcata atatctccgc caaagtgcac ggatctacta acccatataa tatcgtacgt
     8401 gcaacattag atggtttgtc taagttgcat actcctgctg atatcgcagc caaacgtggc
     8461 ttgacagtgg aagacatttt gggagttaac catggctgaa caaaaaaaga ttagggttac
     8521 attggttaaa agcctgattg gtacaattga atctcatcgt gcatgtgcac gcggtttagg
     8581 tttgtgtcgt cgcgagcata cggtagaggt tttagatacc cctgaaaacc gtggtatgat
     8641 taataaaatc agctacttgt tgaaagtgga gtcttgatat gtttttgaat acaattcaac
     8701 ctgctgttgg tgctacgcat gctggtcgtc gtgttggacg cggtattggt agtggtcttg
     8761 gcaaaacggg tggtcgtggt cataaaggtc aaaagagccg gtctggtggg tttcataagg
     8821 tgggtttcga gggtggtcaa atgcccttgc aacgacgcct ccctaaaaga ggttttaaat
     8881 ctttaacagc atcagctaat gcacagcttc gtttaagtga actggaatca attgctgtta
     8941 atgagattga tattttggtc ttaaagcaag cgggtctgat tgcatctaca gtctctaatg
     9001 ttaaagttat tgcttctggt gaaatttcta aggcagttgc tttgaagggt attaaagtta
     9061 ccaaaggtgc gagagctgct atcgaggctg ttggtggtaa gattgaaatg taaggtttaa
     9121 tattgtggct aatcaacaaa cgtcatcagg ttcatccaaa tttggagatc ttaagaaacg
     9181 tcttttgttt ctatttggag cattgattgt ttttcgaatt ggtgcccata tacccgtacc
     9241 tggagttgat gctgttgctt tagctaaatt atacgaaagc gctggaaacg gcatcctggg
     9301 aatattgaat atgttttccg gtgggtcgtt agagcgcttt agtatatttg caataggaat
     9361 tatgccatat atttcagctt ctattattgt acagctcgct tctgaaattt tgccatcatt
     9421 gaaggcttta aaaaaagaag gggaggctgg tagaaaggta attacgaaat atactaggta
     9481 tggtactgtt ttgttagcaa ttcttcaaag tctaggtgtt gcatctttcg tatttcagca
     9541 aggaattgtt gtaacaagtt catttgagtt tcatgtttcc acggtagttt ctttggtaac
     9601 gggaaccatg tttcttatgt ggcttgggga gcaaattact gaaaggggta tcgggaacgg
     9661 tatttcttta atcattacgg caggtattgc ttcaggtatt ccttcgggta ttgcaaagct
     9721 ggttacactg acgaaccaag gttctatgag catgcttacg gcgttgttta ttgtatttgg
     9781 tgccttatta ttaatttatt tggttgtata ctttgaaagt gcacagcgga agattcctat
     9841 tcattatgca aaacgccagt ttaatggtag ggcgggtagt caaaatacgc atatgccttt
     9901 caagttgaat atggctggtg ttattccccc aatttttgct tccagtatta ttctatttcc
     9961 atctactctt ttaggttggt ttggttcggc tgatacaaat agtgttttgc acaaaatagc
    10021 tggattgtta caacacggtc aattgctgta tatggcttta tttgcagcga cagttatttt
    10081 cttttgttat ttttatacgg ctttggtttt tagccctaaa gaaatggcag agaatttaaa
    10141 aaagagtggt gcttttgttc ctgggattag acctggtgag cagacctcta ggtatttaga
    10201 aaaagttgta ttacgtttga cattgtttgg agctctttat attacaacta tttgtttaat
    10261 tccagagttc ttaactacgg ttttaaatgt acctttttat ttgggtggca cgtctttgtt
    10321 gattctagtt gttgtaacga tggattttag tacacaaata aattcgtata ggcttactca
    10381 acagtatgat aagttaatga ctcgttcaga aatgaaatca ttttctcgga aatagaatta
    10441 tggcgaaaga agatactatc caaatgcaag gtgaaattct tgaaacttta cctaatgcaa
    10501 catttaaagt aaaacttgag aatgaccata ttgtattggg tcatatttct gggaagatgc
    10561 ggatgcatta cattcgtatt tctccgggag ataaggtcac agtagagctg acaccttatg
    10621 atctaactag ggctcgaatc gttttcagag caagataaac caataaaagg aaaataaaat
    10681 gcgtgtacaa ccatctgtta agaaaatttg ccgaaattgc aagattattc gtcgaaatcg
    10741 tgtagttcgt gtaatttgta ctgatctccg tcacaaacag cgtcaaggtt aatggaatat
    10801 ttcttttaat gtgattctgt gatatagtga cacactttgc cctaaaaagg aaaaaatatg
    10861 gctcgtattg caggggtaaa tatccctaat aacgcacaca tcgtaattgg tcttcaggct
    10921 atttacggta ttggtgctac tcgtgctaaa ttgatttgtg aggctgcaaa tattgcgcct
    10981 gatactaaag caaaagattt ggacgagact caattagatg ctttgcgtga ccaagttgcc
    11041 aagtatgaag tagaaggtga tttgcgtcgt gaggtaacta tgagtatcaa gcgattgatg
    11101 gacatgggct gctatcgtgg cttccgtcat cgtcgcggct taccatgccg cggtcaacgc
    11161 actcgtacaa atgcgcgtac ccgcaaaggt ccgcgtaaag cgattgctgg taagaaataa
    11221 attttaagga attttattaa tggctaaagc aaacacagct tcacgtgtac gtaaaaaagt
    11281 acgtaaaacc gtgagtgagg gtattgtgca cgttcatgca tctttcaaca ataccatcat
    11341 tacaatcact gaccgtcaag gcaatgcgtt gtcttgggct acctctggcg gcgctggttt
    11401 taaaggttct cgtaaaagta caccatttgc agcacaagtt gcagcagaag cagctggtaa
    11461 agttgcccaa gagtatggcg ttaaaaattt agaggttcgt attaaaggtc caggtccagg
    11521 tcgtgaatcc tctgtacgtg ctttgaatgc tcttggtttc aagattacca gcattactga
    11581 cgttaccccg ttgcctcata acggttgccg tccgcctaaa aaacgtcgta tttaatattg
    11641 gagtgatttg aaacatggca cgttatattg gccctaaatg taagttggca cgtcgcgaag
    11701 gtacggattt gtttttgaag agtgcgcgcc gctctttgga ttctaaatgt aaaattgatt
    11761 ccgctcccgg tcagcatggt gcaaaaaaac cgcgtttgtc agactatggt ttgcagttgc
    11821 gtgaaaaaca aaaaatccgc cgtatttatg gcgtattaga acgtcagttc cgtcgttatt
    11881 tcgcagaagc tgatcgtcgt aaaggttcta ccggcgagtt gctgttgcag ttgctggaat
    11941 ctcgtttgga taatgtcgtt tatcgtatgg gtttcggttc tacccgagct gaagcaagac
    12001 agcttgtttc tcataaggcg atagttgtga atggacaagt tgtcaatatt ccttctttcc
    12061 aagtgaaagc tggtgatgtt gtctcagttc gtgaaaaagc caaaaaacag gtacgtattc
    12121 aagaagcatt gggtttggca actcaaatcg gcttgccggg ttgggtttct gtagatgcgg
    12181 ataaacttga gggtgtgttc aaaaacatgc cggatcgctc ggaattgacc ggtgatatta
    12241 atgaacagct ggtggtagag ttctactcta aataatgcta gctcagtgag ggacagttaa
    12301 atgcagaata gcacaaccga atttttgaaa cctcgtcaaa ttgatgtaaa tactttttct
    12361 gcaactcgtg caaaagtatc tatgcagcca tttgaacgtg gtttcggtca taccttaggt
    12421 aatgctttgc gccgtatctt actgtcatcc atgaatggtt ttgctcctac tgaagtagct
    12481 attgccggtg tattacacga atattctact gttgatggta ttcaggaaga tgttgttgac
    12541 attttgctga atattaaagg tattgtgttt aaactccatg gtcgtagcca agttcaactt
    12601 gtgttgaaga aatcaggttc aggtgtcgta tctgccggtg atattgagtt gccgcatgat
    12661 gtagaaattc tgaatcctgg tcatgtcatt tgtcatttgg ctgataacgg tcaaattgag
    12721 atggaaatta aagtagagca aggtcgtggt tatcaatctg tttcaggtcg tcaggtagtt
    12781 cgtgatgaga accgtcagat tggtgcaatc cagttggatg cgagcttttc gcccatcagc
    12841 cgtgttagct ttgaggttga acctgcacgt gtagagcagc ggacggatct tgataagttg
    12901 gttttggata tcgaaaccga cggttctatt gatcctgagg aagctgtacg cagtgcggca
    12961 cgtattttga ttgatcagat gtctattttt gctgatttgc agggtactcc tgtggaggag
    13021 gttgaagaaa aagcacctcc tatcgaccct gttcttttgc gtccggtgga tgatctggaa
    13081 ttgacagtac gttcagctaa ttgtttgaaa gctgaggata tttattatat tggcgatttg
    13141 attcaacgca ctgaaaccga gcttcttaaa acgccgaatt tgggacgtaa atctttgaat
    13201 gagattaagg aagtattggc atctaaaggt ttgacactgg gttctaagtt ggaagcatgg
    13261 ccacctgtag gcttggaaaa gccttaatga agaattaaag gataattgat atgcgtcatc
    13321 gtaatggcaa tcgcaaatta aaccgtacca gcagtcatcg tgctgcaatg ctgcgtaata
    13381 tggcgaattc attattgact cacgaaacta ttgtaacaac tctgcctaag gccaaggaat
    13441 tgcgccgtgt agtagagccg ttgattacat tgggtaaaaa gccgtcattg gcaaaccgcc
    13501 gtttggcatt tgaccgtact cgcgaccgtg atgttgtagt aaaactgttt ggcgatttgg
    13561 gtcctcgttt tactgctcgt aacggtggtt atgttcgggt gttgaaatac ggattccgta
    13621 aaggtgataa tgcacctctg gcactggttg aattggttga caaaccggct gctgagtaat
    13681 tttagtcata taacgccatc tgccgaaaag caggtggcgt tatttttgca atatctgata
    13741 ggtaataggg tattggctat catgtttaaa atattaattg aatagctaag gtttgcgcgg
    13801 taaacttaca tcattaaaaa attctatgat ggtttatata atgaatgctt tcgatataaa
    13861 gtcgacaaag atggacgtat tgtctatatc tttgcatacg tcagacttgt ttgatttgga
    13921 agatgtgctg gtcaaattgg gcaagaagtt tcaagagtct ggtgttgttc catttgtgct
    13981 ggatgttcaa gagtttgatt atcccgagtc tttggatctt gctgcattgg tttcgttgtt
    14041 ttcaaggcat ggtatgcaaa ttttgggtct gaagcattct aatgaacgtt gggctgctgc
    14101 ggctatgaag tatcatttgc tgttttgtct gtctcattcg gaaaatgtta aagaactggg
    14161 tcaggttgag gtgcagaaca cggaggatgg tcagaaagca aggaaaacag tattgattac
    14221 atcccctgtc cgtaccggtc agcaggttta tgccgaagat ggcgatttga ttgttacggg
    14281 ggcggtcagc cagggggcgg aattgattgc agatggcaat atgcatattt atgcgccgat
    14341 gagagggcgt gctttggccg gtgccaaagg cgatacttct gcccgcatat ttatccactc
    14401 catgcaggca gaactggttt ctgtggcggg tatttaccgt aattttgaac aggatttgcc
    14461 ggaccatctg cacaagcagc cggtacagat attgttgcag gataaccgat tggttatcag
    14521 tgcaattggc tcagaataat tgtttgatat tttaaaaagg aaatattgtg gcaaaaatta
    14581 ttgtagtaac ttcaggtaag ggcggtgtcg gtaaaacgac taccagtgcc agtattgcga
    14641 caggtttggc attacgcgga tataaaactg cggtaattga ttttgatgtg ggtttgcgta
    14701 acctcgacct cattatgggt tgcgagcgtc gtgtcgttta tgacctgatc aatgtcattc
    14761 agggggaggc gacgctcaac caggctttga ttaaagataa aaattgtgaa aacctgttta
    14821 ttttgccggc ttcccagact cgggataaag acgctttgac acgcgaggga gtagaaaaag
    14881 tgatgcagga gctgtccggc aagaaaatgg gctttgagta tattatttgc gactctcctg
    14941 ccggtattga gcagggtgca ttgatggcgt tgtattttgc tgatgaagcc attgtaacga
    15001 ccaatcctga ggtttccagt gtgcgtgact ccgacaggat tttgggaatt ttgcaaagca
    15061 aatcccataa ggcagagcaa ggcggttcgg ttaaagaaca tctgttgatt acgcgttatt
    15121 ctcccgaacg tgtggcaaaa ggcgaaatgc tgtctgtaca ggatatttgc gatattctgc
    15181 atatcccttt gctgggtgtg attcctgaat cccaaaacgt cttgcaggca tccaattccg
    15241 gagaaccggt catccatcag gacagcgtgg cggcttccga ggcatataag gacgttattg
    15301 cccgtctttt gggcgagaac cgtgaaatgc gcttcttgga agctgagaaa aaaagcttct
    15361 tcaaacgtct gtttggagga taaggtatgt cattaatcga atttttattc ggcagaaagc
    15421 agaaaacggc aaccgttgcc cgcgaccgcc ttcaaatcat cattgcccaa gagcgcgccc
    15481 aagaaggtca ggctccggat tacctgccga ctttacgtaa agagttgatg gaagtcctgt
    15541 ccaaatatgt gaacgtttca ttagacaata tccgtatttc ccaggaaaaa caggatggta
    15601 tggatgtgct tgagttgaac attactttgc cggaacagaa aaaggtatag gacatgacct
    15661 taaccgaatt gcggtacatc gtcgcagtcg cccaagaacg tcatttcggc agggcggcgc
    15721 ggcgttgttt tgtcagtcag cccactttgt ctattgccat taagaaattg gaagaagagc
    15781 ttgccgtctc tttgtttgac cggagcagca acgatattat tacgaccgag gcgggggaac
    15841 gtatcgttgc acaggcgcgt aaggtattgg aagaggcgga gcttatcagg catttggcaa
    15901 atgaagaaca aaacgagctg gagggtgcgt tcaaactcgg gctgattttt acggttgcgc
    15961 cgtacctgct gccgaaactg attgtctcgt tgcgccgtac tgcaccgaaa atgcctttga
    16021 tgttggaaga gaattacacg catactttga ccgagtcgct caaacgcggg gacgttgatg
    16081 cgattatcgt tgccgaaccg tttcaagagc cgggcattgt taccgaaccc ttgtatgacg
    16141 aaccgttttt cgtgattgtc ccgaaagggc attcatttga ggaactggat gccgtttcgc
    16201 cccggatgct gggtgaggag caggttttgc tgctgacgga aggcaactgt atgcgggatc
    16261 aggtactctc aagctgttcc gaattggcgg cgaaacaacg catacagggg ttgaccaata
    16321 cattgcaggg cagctcgatc aatacaatcc gccatatggt cgccagcggt ttggcaatca
    16381 gcgtgttacc ggcaaccgcg ctgaccgaaa acgatcatat gctgttcagc attattccgt
    16441 ttgaaggtac gccgccaagc cggcgggtcg tattggcgta ccgccgcaat tttgtccgtc
    16501 cgaaggcgtt gtcggcgatg aaggcggcga ttatgcagtc gcagcttcac ggggtaagtt
    16561 ttatccgcga ctaggcgcag gcattgtttt caaaacgcca tttccctgag ccgacaacac
    16621 ggtatgccaa gatattgccg tcatcatcga ttttgagtat agcatcgcca cggaaactgc
    16681 cgtcctgaag atattcgact tttgcatcac tgtgaatgtt ttcatcagtg ccgatgcaat
    16741 gccatgtata gtggattaac aaaaaccagt acggcgttgc ctcgccttgc cgtactattt
    16801 gtactgtctg cggcttcgtc gccttgtcct gatttttgtt aatccactat aaaagaggcc
    16861 gtctgaaaaa catttttcag acggccttgt tgattcaatc aaatcagtct ttcaacttcg
    16921 acaactgagt ttgcactttt gccattttgt cttccaattc cgccaaatcg gctttgtctt
    16981 tttccaccaa atgcgccggg gctttttcgg tgtagccggg tttggagagt ttggcgttga
    17041 gtttgtccaa ggctttttgc agcttttcgg cttctttgct caaacgggcg gtttcggtgg
    17101 ctttgtcgat ttcgactttc agcatcaggc gcgcgccgtt gcagacggcg acgggcgcgt
    17161 cttcgctttc gggcagggtg gcgacttgct gtgcttcggt cagtcgggtc atcatcggca
    17221 ggtatttgag gtagtccgcc aagtcgtctg cgctttcgac aaacagcggg gctttcacgt
    17281 tgggctggat gcccatttcg ccgcgcaggt tgcggactgc gccaatcaaa tcctgtaaca
    17341 cggtcatttg cccgaatgcc gtctgaacaa tatcgccgcc gtcggcttcg gggaagcggg
    17401 cgagcatgat gctgtcggcg gttttcgcgt cgcacatagg ggcgacggtt tgccacagtt
    17461 cttcggtgat gaacgggata atcgggtgca gcaggcgcag ggcggcttcg agtacgcgca
    17521 ataaggtatg gcgtgtggcg cgttggcggc tggcgcagcc ggtttggagc tgcactttgg
    17581 caagttccag ataccagtcg caatagtcgt tccacacgaa gctgtacagg gtttccgccg
    17641 ccaaatcaaa gcggtaggtt tcgtaggctt gcgttacttg ctcgatggtc tgattcagac
    17701 ggcctacaat ccacatatcg gggaaggagt agccgcgcgg ttcggcagcg gttgcgccgt
    17761 aaccgcagtc ttggttttcg gtgttcatca agacgaagtt ggtggcgttc cagattttgt
    17821 tgcagaagtt gcggtagcct tcggcgcgtt tgaagtcgaa gttgaccgaa cgccccaagc
    17881 tggcgtagct cgccatagtg aagcgcaaag cgtccgcgcc catactcgga atgccttcgg
    17941 ggaagagttt tttcgtggct tcttccactt tcggcgcggt ttcgggtttg cgcaggccgg
    18001 tggtgcgttt taccagcagt ttttccaagc cgatgccgtc gatcaaatcc acagggtcaa
    18061 tgacgttgcc ttcggatttg gacatttttt tgccttcgtg gtcgcgcacg atgccgtgga
    18121 tatacacgtc tttaaacggt actttgccgg tgaagtgggt ggtcatcata atcatacgcg
    18181 ccacccagaa gaagatgatt tcgtagccgg taaccaagac gttggacggc aggaaggctt
    18241 tgagttcgtc ggtttcagac ggccagccga gcgtggagaa cggcacgagc gcggaggaga
    18301 accaagtgtc caatacgtct tcttcgcggg tcaagcctgt tttgccggct tgtttttcgg
    18361 cttccacctg attgcgggcg acgtaaacat tgcctgcttc gtcgtaccac gcggggattt
    18421 gatgccccca ccacagttgg cgcgagatgc accagtcttg gatgttgttc atccattggt
    18481 tgtaggtgtt gacccagttt tcggggataa agcgtaccgc gccgctgtcg acggcttttt
    18541 tggctttgtc ggcgaggctc aagcctttga attcgctgtc aggctcgccg ccgtttggcg
    18601 tagcggacat ggcgacaaac cattggctgg tcagcatcgg ctcaatcact gagcctgttc
    18661 ggtcgccttt cggcgtcatc agcgtgtgcg ctttgatttc gaccaagaaa ccttgttcct
    18721 gcaaatcggc aaccatttgt ttgcgcgcgg caaagcggtc caagcctgcg tatttttcag
    18781 gcagggcaaa gcttggttgc gcttcgcctt tgaagttgaa cacttcggcg tttgccaata
    18841 ctttggcttc caagtcgaac acattaacca agcgcgtatc gtggcgtttg ccgacttcgt
    18901 agtcgttgaa gtcgtgcgca ggcgtgattt tcacgcagcc ggtgccgaag tctttttcaa
    18961 cgtattcgtc ggcaatcacg ggaatggtgc ggccggtcag cggcaggatt aattctttgc
    19021 cgattaagtg ggtataacgt tcgtcttcgg gattgacggc aacggcaacg tcgcccaaca
    19081 gcgtttcggg acgggtggtg gcgacgatga cggcttcggc aggattgtcc gccagcggat
    19141 agcggatgtg ccacatagag ccttgttctt ccacgctttc cacttccaaa tcagacactg
    19201 cggtacccaa taccggatcc cagttcacca agcgtttgcc gcgataaatc aagccctgtt
    19261 catacaggcg tacgaacact tcggttacgg tttcggcgcg tacatcgtcc attgtgaaat
    19321 actcgcgcgt ccagtcggca gaacagccca cacggcgcat ctgctgcgta atcgtgccgc
    19381 cggaaacttc tttccattcc cacacttttt ccaagaattt ttcacgatcc aagttatgac
    19441 gggacacgtt ttgcgcggca agctgacgct cgaccacgat ttgcgtggcg atgcccgcgt
    19501 ggtcggtgcc gggaatccag gcggtgttgc agcctttcat acggtagtag cgggtcaggc
    19561 cgtccataat ggtttggttg aaggcatgac ccatgtgcag cgtgccggtt acattgggcg
    19621 gcggcagttg gatggaaaaa gacggtttgg tcaaatccat atcaggttgg aaatagcctt
    19681 ggctttccca gttttgatag tgcttggatt cgatttcggc gggggagtat ttgtctaaca
    19741 tgatggaact ttgtgaaatt aaggttattt ttgatgtgcg gattataacg caaaaaggcc
    19801 gtctgaatca tttcagacgg cctttggcat acaggtttta aaaatggaac aataccaggc
    19861 tgacggcaat caccgccatg cccattgtca ggccgtaaac ggtttcatgg ccgtctgaat
    19921 agcgtttggc agccggcagc agctcgtcca acgccaaaaa caccatcaca ccggctatca
    19981 cgccgaatac cgaaccaaac acggcaggcg acaaaaacgg ctgcaaaacc aaatagccca
    20041 aagccgcccc caacggctcg gccaagccgg atagcagaca cgcccacacc gttttcttac
    20101 ggctgcgggt ggcaaaataa accggcgcgg cgatggaaat gccctccgga atattatgga
    20161 tggcaatcgc caaggccaaa ggcatcccga ctgctggatt ttccaatgtg gcaaaaaacg
    20221 tcgccaagcc ttcggggaaa ttgtgcgcag taatcgcaaa cgccgccatc atgccgactc
    20281 gcgcgatatg gcggcgtttg ctttcttgaa acgacgggtc ttgcgcgtct aaagtttcat
    20341 gcgggttcgg caccagacgg tcaatcagcg caatgccgcc catcccggcc aaaaatgcca
    20401 tggtcgccgc cgcaaacgcg tggtctttat cataaatttc agcgaacgcc tcgctggact
    20461 tactgaaaat ctccgtcagg gaaacatata ccatcgcacc gccggcaaac gccaaaccaa
    20521 acgacaacac acgcggattg ggcgttttgg aaaacatcac caagccactg cctaatacgg
    20581 taaacaaacc ggcagccaat gtgatggaaa aggcaacggc caaattggac atcgaaaaat
    20641 cgggcatgag aaaacctgcg ctaaaagctg ggacaggttc agactaacac tttttaatgt
    20701 atatgataat agttattatt tattttattg attggataca cggattttga aacaaaaggc
    20761 cgtctgaaaa atgattttca gacggccttt aaatttgaaa tgccgctaaa ccttatcgct
    20821 ttccagctta agcctgataa cgcgacaggc tcaaatcgtc gctgcggatt tcggtgtctt
    20881 tgccgctcac gatatcggcg gttaattttg ccgaacccag cgacatggtc cagcctaaag
    20941 taccgtggcc ggtattcaga aacaggttgt caaagcgggt gcgaccgatt aacggcgtgc
    21001 tgtcgggcgt catcggtctg aggccgctcc agaacgatgc ttggctcaaa tcgccgcctt
    21061 ccgggaacaa gtcgttgacg accaaagcca aggtttcgcg gcgtttttcg ggcagtttga
    21121 tttcgtagcc cgacaattcc gccataccgc cgacgcggat tctgttgtca aagcgcgtga
    21181 tggcgacttt gtagctttca tctaaaacgg tggacaccgg tgcgccgtct gaattggtga
    21241 ccggcagggt caaggaatag cctttgacgg gataaatggg cagattgaga tccaactgcg
    21301 ccaacacagt cctactgaag cagccgagcg cacagacaac ggcatctgct tcaaaccgcc
    21361 ctgtttcggt ttcaacggct ttgatgcgca gcccgttatg gtcgatgcgg ctgatggttt
    21421 ggttgaaatg aaactgtacg cccttttcct gacacaattt gtataggttt tcagtgaaga
    21481 ggtggcagtc gccggtcgca tctgcaggca ggtgcaggcc gccggcaatt ttggcggtaa
    21541 cgcgtgccag cgcaggctca aattctgcac attcttcggg tttcagacgg cggtacggca
    21601 cgccgtagcg ttccaaaacg gcaatgtctt gttctgccgc ttcgacttct ttggtttggc
    21661 ggaaaatctg caacgtccct tttttgcgtt cctcaaaatt catgccggtt tgcgcttcaa
    21721 aacggcggaa catttcacgg ctatattcgg aaatcctgac catgcgctct ttattggttt
    21781 gatagtgcgc tgccgtgcag ttttgcagca tttgccacag ccattcgatt tgatacaggc
    21841 tgccgtcggg gcgaaacagc aaaggcggat ggcttttaaa cagccatttc agcgctttgg
    21901 tcgggatacc gggtgcagcc caaggcgtgg tatagccgta agaaagctgg cctgcgttgg
    21961 caaaactggt ttccatcgcc acaccctcgg cgcggtcgat gaccgttact tcatgtcctg
    22021 cctctgccag ataccacgcg gaagacacgc cggcaacacc cgcacctaaa acaagcactt
    22081 tcatgtttct ccctccggct ttttcaaaac agacttaata tgccgtgccg tctgaatatt
    22141 cggattcaga cggcctcgga tattaatgcg gcaattcgcc gtttgtgatt ttttgtttga
    22201 agtcgcgcgt ttcattgacg atgactttcg ccatcaataa aagtgcaatc aggttgggca
    22261 atgccatcaa gccgttgaat gtgtccgaag ccagccacac caaatcaagg ctcaacacgg
    22321 tacccagcat aacggaagaa acataaccga cgcggtacaa accggcaaat ttctcgccga
    22381 aaacatacac cgcgcatttt tcgccgtaat agcaccagcc caaaatggtt gagtaggcaa
    22441 agaaaatcag gccgatggta acaatccagc cgccgatgcc gggcagcatt ttttggaatg
    22501 tgacggttgt cagtgccgcg ccgctcactt caggtttgac aaactcgccg cccgcgccga
    22561 gcagtcccat taccaacacg atgccggtaa tcgagcaaac gacgatggta tccaaaaacg
    22621 taccggtcat agaaaccaag gcctgacgga cgggatggtc ggttttcgcg gctgcggcgg
    22681 caatcggtgc agaacccatg cccgcctcat tggagaacac gccgcgcgcc acgccgtagc
    22741 ggatgaccgt accgatagcg ccgcccgcca ctgcctgcgc gctgaacgca tcggagaaaa
    22801 tcagcttgac ggcaggcatc agtgcatcgg aattaatcgc gataatggaa agaccgccca
    22861 acacataaaa caccgccata gcaggcacga tgaaagaagc ggctttggcg atgcctttaa
    22921 taccacctaa aacgacaacg gcagtcagaa cggtcaacgt aatgccggta taggcaggtt
    22981 cgataccgaa gctggtttgc accgcctgtg caaccgagtt ggactgcacc gagctgccga
    23041 taccgaagga agcgaatgtg ccgaacagcg caaacgcgac ggccatccat ttccagtttt
    23101 tgcccaagcc tttttcgatg taatacatcg ggccgccgga catttcgcct ttggaattgt
    23161 tgacgcggta tttcaccgcc aacacgcctt cgccgtattt ggtggccatg ccgaaaatgg
    23221 cggtcatcca catccaaaat accgcgcccg ggccgccggt taccaccgca gtcgccacgc
    23281 cggcgatgtt acccgtaccg atggtggcgg acagcgcggt catcaacgcc gcgaaatggg
    23341 aaatatcgcc ttcgtggcct tcgccgcctt catacttctt tgacggcaca aacgcctgtt
    23401 tcagcgcata gcccaacatc gtgaactgca agcctttcag cataacggtc agcacaatgc
    23461 cggtgccgac cagcagcatc aacataacag atccccagac ccagccgctg acggtttcga
    23521 aaaaggcttt gagattgtct aaaaacactt gcatggcttt ctcctttgtc t
//
LOCUS       DAWHSK010000008        21749 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.008, whole genome shotgun sequence.
ACCESSION   DAWHSK010000008 DAWHSK010000000
VERSION     DAWHSK010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21749
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.008"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..414)
                     /locus_tag="WIC03_000235"
     CDS             complement(<1..414)
                     /locus_tag="WIC03_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HEZ3882798.1"
                     /translation="MEWEFNSYYTLIAATLVLLVGKFLVQKIKFLRDFNIPEPVAGGL
                     VAAIILFALHEAYGVSFKFEKPLQNAFMLIFFTSIGLSADFSRLKAGGLPLVVFTAIV
                     GGFILVQNFVGVGLATALGLDPLIGLITGSVSLTGG"
     gene            complement(632..2038)
                     /locus_tag="WIC03_000236"
     CDS             complement(632..2038)
                     /locus_tag="WIC03_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HEZ3882799.1"
                     /translation="MTAAPDILYRQAAALLEQSNTAQALPLLQQAAEQGYAEAAFVLG
                     NHLLQNGQPEQALSWLEAAAAQRHPKALFSLLQQREHNGTPTGQLLNDYAWLGEQGHS
                     EAQLILMRYHAQRNDPQSLYWAELAAARYAAPAYYHLARHHQRQGDVETAIEQYEKAA
                     ALGVTAACWQLGQIYFYGTGVSPNHAQAEHYLEPAAQAGHIAAQTLLADLLAAQRKPE
                     ALEWYRRAANKEQAEAQAKLAQYALTGELSERDPFQAARYAKAAAEKNHPEALKIMGD
                     LYRYGLGIKADNHIAHDYYHRAAALGSAAAAQKLISDAALYHPQQYEQIKTAALQQQQ
                     TETIYRLAETQAHAIGRPADYNAARKNYMEAAEFHHKNAAAALGRIYHYGLGTAQDPR
                     AAAHWYAIAAEQNHPSAQYHLACFYYHGQGVGCHVPTACYWLQAAISNGHTSAESLIS
                     LLEQWRREAHHAIGQKAV"
     gene            complement(2405..3664)
                     /locus_tag="WIC03_000237"
     CDS             complement(2405..3664)
                     /locus_tag="WIC03_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide modification protein
                     LipB"
                     /protein_id="HEZ3882800.1"
                     /translation="MKQTVLKNNLQNLLESAENILLLQGPVGDFFLRLADWLTANGKT
                     VHKFNFNAGDDYFYPPTQAHTVVFNDNYDAFPEFLQEYITQHHIQAVVCFGDTRPYHV
                     IAKRIANENQASFWAFEEGYFRPYYITLEKDGVNAFSPLPRRADFFLEQFPKLAQQEY
                     KAPTPVHGGFTPMAKNAIRYYIELFRNPRKYPDYIHHRAPNAGHYLKPWSLSILKRLN
                     YYIEDIQIAKRVEAGKYGKFFIVPLQVFNDSQVRIHCDFPSVRSFLLHVLSSFAEHAP
                     ADTNIIIKHHPMDRGFIDYWRDIKRFIKEHPELKGRVIYVHDVPLPVFLRHGLGMVTI
                     NSTSGLSGLIHNMPVKVLGRAYYDIPGITDQNTLAEFWNHPTPPDKELFHAYRMYHLN
                     VTQINGNFYSQVFFPNKKTSNSSTPVI"
     gene            complement(3801..5849)
                     /locus_tag="WIC03_000238"
     CDS             complement(3801..5849)
                     /locus_tag="WIC03_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HEZ3882801.1"
                     /translation="MIPAYIPLRSFGIRKIPNITQFLPEYSLNKTVFNAQTIIGWGLR
                     PTTHQARAFAQSHGLPFVALEDGFLRSLGLGVAGYPPFALVYDDIGIYYDTTRPSRLE
                     QLILAANMPSEMLNQAQQAIDLILTHRLSKYNHALDFSDGLVQDSKNIVLVIDQTFGD
                     MAIQYGGADEATFRQMFQTALAENPDAEIWVKTHPDVLSGKKQGYLTDLAQQPRVRLL
                     AEDINPISLLQAVDKVYCVTSQMGFEALLCGKPLVTFGLPWYAGWGVSDDRHPKIDSL
                     VQTQRRAPRNLLQLFAAAYLQYSRYLNPNTGEAGSLFDVIDYLATVKRKNDKLRGELY
                     CVGMSLWKRAVAKPFFNVPSCRLKFISSTQKLAGVKLSDDARILAWGNGKEAIVRFAE
                     QHHIPLLRMEDGFIRSVGLGSNLVPPLSLVTDDMSIYFNAETPSRLEYILQNQNFDDQ
                     DFQTALKLQKMLTENHISKYNVGSSDFTAPSTDKTVILVPGQVEDDASIRYGSPQIYR
                     NLDLLRTVRERNPNAYIIYKPHPDVVSGNRIGHISPEDAARYADQTAEQTDILTCLQY
                     ADEVHTMTSLTGFEALLRGKKVSCYGLPFYAGWGLTQDLLPIPRRSRRLELWQLIAGT
                     LIHYPDYIHPETHQAINAETAAQILIRQKNMQKNNNGLHRGYFAKKLGKIKQLYRSFK
                     "
     gene            complement(6011..6550)
                     /gene="rfbC"
                     /locus_tag="WIC03_000239"
     CDS             complement(6011..6550)
                     /gene="rfbC"
                     /locus_tag="WIC03_000239"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEZ3882802.1"
                     /translation="MNIIDTRIPDVKLLEPKVFGDERGFFMETFRDEWFRQNVAERTF
                     VQENHSKSTQGVLRGLHYQTENTQGKLVRVVSGEVFDVAVDMRRDSPTFGHWVGEVLS
                     ADNKRQLWVPEGFAHGFYVLSDEAEFVYKCTDYYNPNAEHSLIWNDATVGIDWPLLGE
                     PSLSAKDLAGKMLAEAATF"
     gene            complement(6605..7480)
                     /gene="rfbA"
                     /locus_tag="WIC03_000240"
     CDS             complement(6605..7480)
                     /gene="rfbA"
                     /locus_tag="WIC03_000240"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEZ3882803.1"
                     /translation="MKGIILAGGSGIRLYPITRGVSKQLLPVYNKPMIYYPLSVLMLA
                     GIRDVLVITTPEDNDAFRRLLGDGSDFGIQISYAIQPSPDGLAQAFLIGEEFIGDDSV
                     CLVLGDNIFYGQHFSQSLKEAAASVETKGATVFGYQVKDPERFGVIEFDENFKALSIE
                     EKPKQPKSDWAVTGLYFYDNRVVEFAKQVKPSERGELEITTLNQMYLDDGSLNVQLLG
                     RGFAWLDTGTHASLLEAASFVETIENIQNLQVACLEEIAWRNGWLSSEEIRNLAKPMC
                     KNEYGRYLLRLVGKD"
     gene            complement(7693..8769)
                     /gene="rfbB"
                     /locus_tag="WIC03_000241"
     CDS             complement(7693..8769)
                     /gene="rfbB"
                     /locus_tag="WIC03_000241"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEZ3882804.1"
                     /translation="MQTANKKTILVTGGAGFIGSAVVRHIIRNTRDAVVNVDKLTYAG
                     NLKSLTEVADNPRYAFEQVDICDRAELDRVFAQHRPDAVMHLAAESHVDRSIDSAGEF
                     IQTNIIGTFNLLEAARAYRRQMPSEQREAFRFHHISTDEVYGDLHGTDDLFTETTPYA
                     PSSPYSASKASSDHLVRAWLRTYGLPTIVTNCSNNYGPYHFPEKLIPLMILNALDGKP
                     LPVYGDGMQIRDWLFVEDHARALYQVVTEGEIGETYNIGGHNEKANIEVVRTICALLE
                     ELVPNKPQGVARYEDLITYVKDRPGHDVRYAIDAAKIGRELGWQPQETFESGIRKTVE
                     WYLNNQTWWSRVLDGSYNRERLGS"
     gene            complement(8770..9813)
                     /gene="galE"
                     /locus_tag="WIC03_000242"
     CDS             complement(8770..9813)
                     /gene="galE"
                     /locus_tag="WIC03_000242"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEZ3882805.1"
                     /translation="MNKQYPKINYIGNKEKIASWICDQPPSDVDTVADVFSGGCSFAY
                     EAKKRGYRVITNDILAINYQIALALIENNHETLNDDDVAMIFSGSPHAGFMSQRYAEK
                     FYFHDECQQLDLYRKNIGKLDNQYKRALAFTLMRRAMIRKMPYTEDMRPGDTANPYGA
                     SKAMVERMLTDIQKADPRWSVILLRYFNPIGAHESGLIGEQPNGIPNNLLPYICQVAS
                     GRLPQLSVFGGDYPTPDGTGMRDYIHVMDLAEGHIAAMKAKGGVAGVHLFNLGSGRAY
                     SVLEIIRAFEAASGLNIPYQIQPRRAGDLACSFADPSHTKQQTGWETKRGLKQMMEDS
                     WRWVSRNPNGYGD"
     gene            complement(9806..10808)
                     /gene="dcm"
                     /locus_tag="WIC03_000243"
                     /pseudo
     CDS             complement(9806..10808)
                     /gene="dcm"
                     /locus_tag="WIC03_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239422.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            complement(10839..13112)
                     /locus_tag="WIC03_000244"
     CDS             complement(10839..13112)
                     /locus_tag="WIC03_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEZ3882806.1"
                     /translation="MNITQILSQELSATAAQITAAVELLDDGATVPFIARYRKEATGG
                     LDDTQLRRLAERLQYLRELEERKAVVLKSIEEQGKLSDDLRAQIEAADNKTALEDLYL
                     PYKPKRRTKAQIAREHGLQPLADVLLAEQSQDVEAAAQGYLNENVPDAKAALDGARAI
                     LMEQFAEDAELIGTLRDKLWNEAEIHAQVVGGKETEGEKFSDYFDHREPIRAMPSHRA
                     LAVLRGRNEGVLNIALKYQPDDTPITQQSEYEQIIARRFKVSDGHKWLRDTVRLTWRA
                     KIFLSLELEALNRLKEAADTDAITVFARNLKDLLLAAPAGRLTTLGLDPGYRNGVKCA
                     VVDDTGKLLDTVIVYLHQENNMLATLSRLIKQHGVKLIAIGNGTASRETDKIAGELVR
                     GMPEMGLHKIVVSEAGASIYSASELAAREFPDLDVSLRGAVSIARRLQDPLAELVKID
                     PKSIGVGQYQHDVNQSRLAKSLDAVVEDCVNAVGVDVNTASAPLLARISGLNQTLAQN
                     IVAYRDENGAFDSRKKLLKVPRLGEKTFEQAAGFLRINGGKEPLDASAVHPEAYPVVA
                     KMLAQQGINAAELIGNRERVKQIKASDFTDERFGLPTILDILSELEKPGRDPRGEFQT
                     ASFAEGIHEISDLQVGMILEGVVSNVANFGAFVDIGVHQDGLVHISALSNKFVQDPRE
                     VVKAGDVVKVKVLEVDAARKRIALTMRLDDEPGGAKHKMPSENRSRERTAGRKPQRND
                     RAPANSAMADAFAKLKR"
     gene            complement(13176..13826)
                     /locus_tag="WIC03_000245"
     CDS             complement(13176..13826)
                     /locus_tag="WIC03_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ3882807.1"
                     /translation="MISVEHVSKRYLTRQGWRTVLHDISFKMEKGEKIGILGRNGAGK
                     STLIRLISGVEPPTTGEIKRTMSISWPLAFSGAFQGSLTGMDNLRFICRIYNVDIDYV
                     KNFTEEFSELGQYLYEPVKRYSSGMKARLAFALSLAVEFDCYLIDEVIAVGDSRFADK
                     CKYELFEKRKDRSIILVSHSHSAMKQYCDNAMVLEKGHMYQFEDMDKAYEYYNSLP"
     gene            complement(13823..14620)
                     /locus_tag="WIC03_000246"
     CDS             complement(13823..14620)
                     /locus_tag="WIC03_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_157140410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ3882808.1"
                     /translation="MKALHKTSFWESLAIQRRVIGALLMREIITRYGRNNIGFLWLFV
                     EPLLMTFVIVLMWKFLKADRYSTLNIVAFAITGYPMLMMWRNASKRAVGSISSNASLL
                     YHRNVRVLDTILARMILEIAGATIAQIVIMAVLIAIGWIEMPADMFYMLMAWLLMAFF
                     AIGLGLVICSIAFNFEPFGKIWGTLTFVMMPLSGAFFFVHNLPPKVQEYALMIPMVHG
                     TEMFRAGYFGSDVITYENPWYIVLCNLVLLLFGLAMVSKFSKGVEPQ"
     gene            complement(14620..15783)
                     /gene="ctrB"
                     /locus_tag="WIC03_000247"
     CDS             complement(14620..15783)
                     /gene="ctrB"
                     /locus_tag="WIC03_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export protein CtrB"
                     /protein_id="HEZ3882809.1"
                     /translation="MSEQLPVAVATETKAERKKPKKKSWIKKLSPLFWVTVIIPTVIS
                     LVYFGFFASDRFTSQSSFVVRSPKSQSSLNGLGAILQGTGFARAQDDIYTVGEYMRSR
                     SSLDELRKILPVREFYETKGDAFSRFNGFGFRGEEEAFYQYYKNQVMINFDTVSGIST
                     LNVTSFDALESKKINEALLKQGEALINQLNDRARADTVRYAEEVVKTAAERVKEASQN
                     LTDYRIANGVFDLKAQSEVQMGLVSKLQDELIVIQTQLDQVKAVTPENPQIPGLQARE
                     QSLRKEIDQQLRAISGGGHSSLSNQAAEYQRVYLENQLAEQQLAAAMTSLESAKVEAD
                     RQQLYLEVISQPSLPDLAHEPKRLYNIVATLIIGLMVYGILSLLTASIREHKN"
     gene            complement(15798..16973)
                     /gene="ctrA"
                     /locus_tag="WIC03_000248"
     CDS             complement(15798..16973)
                     /gene="ctrA"
                     /locus_tag="WIC03_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export outer membrane
                     protein CtrA"
                     /protein_id="HEZ3882810.1"
                     /translation="MFKVKFYIRHAVLLLCGSLIVGCSAIPSSGPSAKKIVSLGQQSE
                     VQIPEVELIDVNHTVAQLLYKAQINQSFTQFGDGYASAGTLNIGDVLDIMIWEAPPAV
                     LFGGGLSSMGSGSAHQTKLPEQLVTARGTVSVPFVGDISVVGKTPGQVQEIIKGRLKK
                     MANQPQVMVRLVQNNAANVSVIRAGNSVRMPLTAAGERVLDAVAAVGGSTANVQDTNV
                     QLTRGNVVRTVALEDLVANPRQNILLRRGDVVTMITNPYTFTSMGAVGRTQEIGFSAR
                     GLSLSEAIGRMGGLQDRRSDARGVFVFRYTPLVELPAERQDKWIAQGYGSEAEIPTVY
                     RVNMADAHSLFSMQRFPVKNKDVLYVSNAPLAEVQKFLSFVFSPVTSGANSINNLTN"
     gene            17108..18241
                     /gene="neuC"
                     /locus_tag="WIC03_000249"
     CDS             17108..18241
                     /gene="neuC"
                     /locus_tag="WIC03_000249"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HEZ3882811.1"
                     /translation="MKRILCITGTRADFGKLKPLLAYIENHPDLELHLIVTGMHMMKT
                     YGRTYKEVTRENYQHTYLFSNQIQGEPMGAVLGNTITFISRLSDEIEPDMVMIHGDRL
                     EALAGAAVGALSSRLVCHIEGGELSGTVDDSIRHSISKLSHIHLVANEQAVTRLVQMG
                     EKRKHIHIIGSPDLDVMASSTLPSLEEVKEYYGLPYENYGISMFHPVTTEAHLMPQYA
                     AQYFKALELSGQNIISIYPNNDTGTESILQELLKYQSDKFIAFPSIRFEYFLVLLKHA
                     KFMVGNSSAGIREAPLYGVPSIDVGTRQSNRHMGKSIIHTDYETKNIFDAIQQACSLG
                     KFEADDTFNGGDTRTSTERFAEVINNPETWNVSAQKRFIDLNL"
     gene            18245..18931
                     /gene="neuA"
                     /locus_tag="WIC03_000250"
     CDS             18245..18931
                     /gene="neuA"
                     /locus_tag="WIC03_000250"
                     /EC_number="2.7.7.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acylneuraminate cytidylyltransferase"
                     /protein_id="HEZ3882812.1"
                     /translation="MEKQNIAVILARQNSKGLPLKNLRKMNGISLLGHTINAAISSKC
                     FDRIIVSTDGGLIAEEAKNFGVEVVLRPAELASDTASSISGVIHALETIGSNSGTVTL
                     LQPTSPLRTGAHIREAFSLFDEKIKGSVVSACPMEHHPLKTLLQINNGEYAPMRHLSD
                     LEQPRQQLPQAFRPNGAIYINDTASLIANNCFFIAPTKLYIMSHQDSIDIDTELDLQQ
                     AENILNHKES"
     gene            18932..19981
                     /gene="siaC"
                     /locus_tag="WIC03_000251"
     CDS             18932..19981
                     /gene="siaC"
                     /locus_tag="WIC03_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysialic acid capsule biosynthesis protein
                     SiaC"
                     /protein_id="HEZ3882813.1"
                     /translation="MQNNNEFKIGNRSVGYNHEPLIICEIGINHEGSLKTAFEMVDAA
                     YNAGAEVVKHQTHIVEDEMSDEAKQVIPGNADVSIYEIMERCALNEEDEIKLKEYVES
                     KGMIFISTPFSRAAALRLQRMDIPAYKIGSGECNNYPLIKLVASFGKPIILSTGMNSI
                     ESIKKSVEIIREAGVPYALLHCTNIYPTPYEDVRLGGMNDLSEAFPDAIIGLSDHTLD
                     NYACLGAVALGGSILERHFTDRMDRPGPDIVCSMNPDTFKELKQGAHALKLARGGKKD
                     TIIAGEKPTKDFAFASVVADKDIKKGELLSGDNLWVKRPGNGDFSVNEYETLFGKVAA
                     CNIRKGAQIKKTDIE"
     gene            20065..21552
                     /locus_tag="WIC03_000252"
     CDS             20065..21552
                     /locus_tag="WIC03_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2,8-polysialyltransferase family protein"
                     /protein_id="HEZ3882814.1"
                     /translation="MLKKIKKALFQPKKFFQDSMWLTTSPFYLTPPRNNLFVISNLGQ
                     LNQVQSLIKIQKLTNNLLVILYTSKNLKMPKLVHQSANKNLFESIYLFELPRSPNNIT
                     PKKLLYIYRSYKKILNIIQPAHLYMLSFTGHYSYLISIAKKKNITTHLIDEGTGTYAP
                     LLESFSYHPTKLERYLIGNNLNIKGYIDHFDILHVPFPEYAKKIFNAKKYNRFFAHAG
                     GISINNNIANLQKKYQISKNDYIFVSQRYPISDDLYYKSIVEILNSISLQIKGKIFIK
                     LHPKEMGNNYVMSLFLNMVEINPRLVVINEPPFLIEPLIYLTNPKGIIGLASSSLIYT
                     PLLSPSTQCLSIGELIINLIQKYSMVENTEMIQEHLEIIKKFNFINILNDLNGVISNP
                     LFKTEETFETLLKSAEFAYKSKNYFQAIFYWQLASKNNITLLGHKALWYYNALYNVKQ
                     IYKMEYSDIFYIDNISVDFHSKDKLTWEKIKHYYYSADNRIGRDR"
BASE COUNT         5845 a         4987 c         5085 g         5832 t
ORIGIN      
        1 tccgcccgtc agcgacaccg aaccggtaat cagaccgatg agcgggtcca aacccaaagc
       61 cgtagccagt ccgaccccga caaagttttg caccaagata aatccgccca caatcgcggt
      121 aaaaaccact agcggcaaac cgcccgcctt caaacgggaa aaatccgcgc tcaagccgat
      181 ggacgtgaag aaaatcagca taaacgcatt ttgcagcggt ttctcaaatt tgaagctcac
      241 gccgtacgcc tcgtgcagcg caaacaggat aatcgcggca accaaaccgc cagcgaccgg
      301 ctcgggaata ttgaagtctc gtaagaattt gattttttga accagaaatt taccaaccag
      361 caacacgagc gtggcggcaa tcagtgtgta ataactgttg aattcccatt ccatgttgtt
      421 tcacctccta gaaatatttt tccgagcatc cgtttttgtt ccggatgtag tgtgtttcaa
      481 cgcttggcgt tatccgccga gtgtcatggt attgatgcgc ggacaaggtg ttttcagaaa
      541 gaaatgaaaa caatggcgtg tccgtgaaaa ataactggca aatattaggg gaatagttaa
      601 agattgtcaa aaaatgcgag tgtaaatctt ttcagacggc cttttgtccg atggcatggt
      661 gtgcttcgcg tcgccattgt tctaataggg atattaatga ttcggccgaa gtgtggccgt
      721 tgctgatggc agcctgcagc cagtagcagg cggtcggaac atgacagccg acaccttgtc
      781 cgtgatagta aaaacaggcg aggtggtatt gggcggaagg gtggttttgt tcggcagcga
      841 tggcgtacca gtgtgcagcc gcccgaggat cttgcgccgt accgaggccg taatggtaga
      901 tgcggcctaa ggcagccgcc gcgtttttat ggtggaactc agcagcttcc atgtaatttt
      961 tgcgtgcggc attatagtcg gcgggacgac cgatggcatg ggcttgtgtt tccgccaaac
     1021 ggtagatggt ttcggtttgt tgctgttgca gggcggcagt tttgatttgt tcgtattgtt
     1081 gcggatggta cagcgcggcg tcgctgatga gtttttgtgc tgcggcggca gaacccagcg
     1141 cggcagcacg gtggtagtaa tcgtgcgcga tatggttgtc agctttgatg ccgaggccgt
     1201 agcggtagag gtcgcccatg attttcaggg cttcaggatg gtttttctcg gcagcggctt
     1261 tggcatagcg cgccgcttgg aacggatcgc gttcggaaag ttcgccggtc agggcgtatt
     1321 gggccagctt ggcttgtgct tccgcttgct ccttattggc ggcacgacga taccattcta
     1381 aggcttcggg tttgcgttgg gcggcaagaa ggtcggccag cagggtttgt gcggcgatgt
     1441 ggccggcttg tgcggctggt tcgagatagt gttcggcttg tgcgtggttg gggctgacac
     1501 ctgtaccgta gaagtagatt tgaccaagtt gccagcaggc ggcagttacg ccgagtgctg
     1561 ccgctttttc gtattgttcg atggctgttt caacgtcgcc ttggcgttga tgatggcgtg
     1621 ccagatggta atacgcaggt gcggcatatc gggcggcagc aagttccgcc cagtagagcg
     1681 attgtggatc gttgcgttgc gcgtggtaac gcatgaggat taattgggct tctgagtgcc
     1741 cctgctcacc cagccaggca tagtcgttga gaagctgtcc ggtcggggtg ccgttgtgtt
     1801 cgcgttgttg cagcagggag aagagtgctt tgggatggcg ttgggccgcg gcggcttcca
     1861 accatgaaag tgcctgctcc ggttggccgt tttgcagcag atggttgccc aatacgaaag
     1921 cagcttccgc ataaccttgc tctgccgcct gttgcaacag gggcagggct tgggcggtat
     1981 tggattgttc caaaagggcg gcggcttgcc ggtagaggat atcaggagcg gcggtcatgg
     2041 tttgcgggcg gatataaagt ggaagacgga tgctgcggca tatgggcggc gataccggtt
     2101 gagggctttg taaaaatatt gctcgccaca gttttgtaaa gtgtattgcc ttgctggctt
     2161 atctttagga tgtttggctt gttatattgt tttgcctggg agtacatcca tattttgtgt
     2221 gttattaagg cctcagcttg agtcggtatg cgctcatggc agctcggtca ttatactgtt
     2281 tttgtaattt attgttaatg ataaatgcag atttttgtgt tttatttaca tggcagtagc
     2341 taaggtattg attgtttcag gccgtctgaa atgttttcag acggcctgaa cttccttcgc
     2401 taagtcagat tactggtgtg gaagagttgg aggttttttt gttggggaaa aacacctgac
     2461 tgtagaagtt gccgttaatt tgggtcacgt tgaggtggta cattcggtag gcatggaaca
     2521 gctctttgtc aggcggtgtc ggatgattcc aaaattctgc caaggtattt tggtcagtaa
     2581 tgccgggaat atcataatag gcacggccga gaaccttaac tggcatattg tgaatcagtc
     2641 cggacaggcc gctggtgctg ttgatggtga ccatgccgag accgtggcgc aggaaaacgg
     2701 gcagggggac atcatggaca taaatcacgc ggcctttgag ttcggggtgt tctttgataa
     2761 agcgtttaat gtcgcgccag tagtcgataa aaccgcggtc catcggatga tgcttgatga
     2821 tgatgttggt atcggcaggc gcgtgctcgg caaatgaact caaaacatgg agcaggaagc
     2881 tgcggacgct gggaaagtcg caatggatac ggacttggct gtcgttgaat acctgtaagg
     2941 gaacaataaa aaacttgccg tatttgcctg cttccacacg tttggcgatt tggatgtctt
     3001 caatatagta gttcaaacgc ttgaggatgg agagcgacca cggtttgagg taatggccgg
     3061 cattgggtgc gcggtggtgg atgtagtcgg ggtatttgcg tggattgcgg aacaactcga
     3121 tatagtaacg gatagcgttt tttgccatgg gcgtaaaacc gccgtgtacc ggcgttggcg
     3181 ctttatattc ttgctgggca agcttaggga attgttcaag aaaaaagtcg gcacggcgcg
     3241 gcaacgggga aaatgcgttg acgccgtctt tttctaaggt gatgtagtag gggcggaaat
     3301 agccttcttc aaacgcccag aaactggctt ggttttcgtt tgcaatgcgt tttgcaatga
     3361 cgtgataagg gcgtgtgtcg ccaaagcaga caacggcctg gatgtgatgt tgagtgatgt
     3421 attcttgcaa aaactcagga aaggcatcgt agttgtcgtt aaaaacaacg gtatgcgctt
     3481 gagtgggcgg ataaaaatag tcgtcgcctg cattaaagtt gaatttatgt acggttttgc
     3541 cgtttgcagt cagccagtcg gcaaggcgca gaaaaaaatc gccgacaggg ccttgaagca
     3601 acaggatatt ttctgcgctt tcaagcaggt tttgcaggtt atttttgagg acggtctgtt
     3661 tcatgttgca atgtggtttt gttttttatg taatagtttt aggttgaact ttcaagcata
     3721 cgccaagaga attaacgatg caaaggcaat gatgccgcaa tagaggcaag tttatgacgc
     3781 atcgttaact ttgatggtat ttatttgaaa gatcgatata gttgtttgat tttacctaat
     3841 tttttggcaa agtacccgcg atgtaatccg ttgttgtttt tttgcatatt tttttgtcgt
     3901 atcaggattt gggctgcggt ttctgcattt atggcctgat gggtttcggg gtggatgtag
     3961 tcgggatagt ggatgagcgt gccggcaatc agctgccaaa gctcaagtct gcggctacgg
     4021 cgcggaatgg ggagcagatc ttgggtaagt ccccagcctg cgtaaaaagg caggccgtag
     4081 cagctgactt ttttgccgcg caacaaggct tcaaaaccgg tcagcgaagt catggtatgt
     4141 acttcgtctg cgtattggag acaggtcagg atgtcggttt gctcggcggt ttggtcggca
     4201 tatcgtgcag catcttcagg ggaaatatgg ccgatgcggt taccgctgac tacatcggga
     4261 tgcggtttgt agatgatata ggcattgggg tttcgttcgc gtacggtacg gagcaaatcc
     4321 agattgcggt agatttgggg cgaaccgtag cggatagacg catcatcttc aacctggccg
     4381 ggaacgagga tcacggtttt gtcggttgac ggggcggtga agtctgagct gccgacgttg
     4441 tatttactga tgtggttttc ggtcagcatt ttttgcagct tcaaggccgt ctgaaagtct
     4501 tgatcgtcga agttttggtt ttgtaggatg tattcaagac gggacggggt ttcggcattg
     4561 aaataaatgc tcatatcgtc ggtaacgagc gacagcggcg gcactaagtt ggagccgagt
     4621 ccgaccgagc ggataaagcc gtcttccatg cgcagcaggg ggatgtggtg ttgttcggca
     4681 aagcggacga tggcctcttt gccgttgccc caggcaagga tgcgtgcatc gtcggacagt
     4741 ttgacccctg ccagtttttg ggtggaagag ataaatttca gacggcaaga gggtacgtta
     4801 aagaacggtt tggcaaccgc gcgtttccac aaagacatac caacgcaata caactcgcca
     4861 cgcaatttgt cgtttttgcg tttgaccgtc gccagatagt cgatgacatc aaagaggctg
     4921 cctgcttcgc cggtattggg gttgaggtag cggctgtatt gcagataggc tgcagcgaag
     4981 agctgcagca agttgcgggg ggcgcggcgt tgggtttgaa caaggctgtc gattttagga
     5041 tggcggtcgt cactcacgcc ccatccggca taccacggca aaccaaacgt taccaaaggc
     5101 ttgccgcaca acaaggcttc aaaacccatt tgcgaggtca cgcaatacac tttgtccacc
     5161 gcttgcagca gcgaaatcgg attaatgtct tcagccaata aacgcacacg cggctgctgt
     5221 gccaaatcag taaggtaacc ctgtttcttg ccgctcaata catcgggatg ggttttgacc
     5281 cagatttccg catcaggatt ttcggctaag gccgtctgaa acatttggcg gaatgtggct
     5341 tcatctgcgc cgccatattg aatcgccata tcgccgaaag tttgatcaat taccaaaaca
     5401 atgtttttgc tgtcttgtac gaggccgtct gaaaaatcca gagcatggtt atatttagac
     5461 aaacggtgcg tcaaaatcaa atcaatggcc tgctgcgctt ggttaagcat ttcagacggc
     5521 atgtttgcgg ctaaaatcaa ctgctccaaa cgcgatgggc gggttgtgtc gtaatagatg
     5581 ccgatatcgt cgtaaaccag tgcgaagggc ggataaccgg ccacgcctaa gcccagtgag
     5641 cgtaaaaaac cgtcttccaa ggctacaaac ggcaagccat gtgattgtgc aaaagcacgt
     5701 gcttggtggg tggtcgggcg taagccccag ccaatgatgg tttgggcgtt gaatacggtt
     5761 ttgttaagtg aatattccgg tagaaattgc gtaatattcg gaattttacg tataccgaaa
     5821 gagcgaagag gaatgtaggc aggaatcaca ataaatagga aaaaatatta ctgacaatat
     5881 aatatcttat taacagattt tgtgtaaatt ttctgactat ttaaatatag tagaaattta
     5941 gttagtaggc tagctggtaa aaattaggtc atcttgctac aaattaccca cgatgaccta
     6001 aaagtgaaaa ttaaaatgtg gccgcttcag caagcatctt gcctgccaaa tccttggccg
     6061 ataggcttgg ttctcccaat aaaggccagt cgattcccac tgtggcatca ttccaaatta
     6121 gggaatgttc cgcattcggg ttgtaatagt cggtgcattt gtagacgaat tccgcttcat
     6181 cgctcaacac ataaaatcca tgggcaaacc cttccggcac ccataattgg cgtttgttgt
     6241 ctgccgataa aacctcaccg acccagtggc cgaaagtagg actatcacga cgcatatcca
     6301 cggcaacatc aaagacttcg cctgatacca cgcgcaccag tttgccttgt gtattttcgg
     6361 tttgataatg taaaccgcgt aatacgcctt gggtggattt gctgtggttt tcctgcacga
     6421 aagtgcgctc ggctacattt tggcggaacc attcatcacg gaaggtttcc ataaaaaaac
     6481 cacgctcgtc accgaagact ttgggttcga gcaatttgac atcgggaatg cgggtatcga
     6541 tgatattcat catgttttcc taaaaagcgt ttcagacggc ctgaaaactg gtaaaagcaa
     6601 cctcttaatc tttgccaacc aagcgcaata aatagcggcc atattcattt ttacacattg
     6661 gctttgccaa gtttctgatt tcttcgctgc tcaaccagcc attacgccat gcgatttctt
     6721 ccaaacaggc gacttgtagg ttttgaatgt tttcaatggt ttcgacaaac gaggctgctt
     6781 ccaacaggct ggcgtgtgtg ccggtatcaa gccatgcgaa accgcgaccg agcaattgga
     6841 cattgagcga accatcgtcc aaatacattt gattcaaggt ggtaatttcc aattcgccgc
     6901 gttcagacgg cttcacttgt ttggcaaact cgactacgcg attatcgtag aaatagaggc
     6961 cggttaccgc ccaatcggat ttaggttgtt tcggtttttc ttcgattgac agggctttga
     7021 aattctcatc aaactcaatc acaccgaaac gctcgggatc tttcacttga tagccgaata
     7081 cggtagcacc tttggtttcc acggaagcgg cggcttcttt cagggattgg ctgaaatgct
     7141 ggccatagaa aatattgtcg cccaacacca aacacaccga atcatcgccg ataaattctt
     7201 caccaattaa aaaggcttgc gccaaaccat cggggctggg ttggatggcg taggaaattt
     7261 gaataccgaa atcgctgcca tcacccagta agcggcggaa agcatcgttg tcttcggggg
     7321 tggtaatcac cagcacatcg cggattccgg ccaacatcaa caccgacaag gggtaataaa
     7381 tcatcggttt gttgtacacc ggtagtaatt gctttgatac gccgcgggta atcgggtaaa
     7441 ggcggatacc gctgccgcct gcgagaataa tgcctttcat cagtttactt tccttatgta
     7501 gtttttcagg cggccttcat gccgtctgaa cgtattttta ttatttgagg cctttgcaaa
     7561 gccacagatt tgagtacagt tcgaagttat agcagcgcag aaagcgcaga catatcacaa
     7621 agataggcaa gctttcgagc agcgcataac gaagaaatgt gccaaagatg gggagtttgc
     7681 aaaggtctct atttaactgc ctaagcgttc acgattgtag ctgccgtcta acacgcgcga
     7741 ccaccaagtt tgattgttta aataccattc cactgttttg cggatgccgc tttcaaaagt
     7801 ttcttgcggc tgccaaccca actcacggcc gatttttgcg gcatcaatcg catagcgcac
     7861 atcatggccg gggcggtctt tgacataggt aatcaaatct tcataacgcg ccacgccttg
     7921 cggtttgttc ggcaccaatt cttccagcag ggcgcaaatg gtgcgcacca cttcaatatt
     7981 ggctttttca ttgtggccac caatgttgta agtttcgccg atttcgcctt ctgtgaccac
     8041 ttgatacagc gcacgcgcat gatcttcaac aaacagccag tcgcggattt gcataccgtc
     8101 gccatacacc ggcagcggtt tgccatcaag cgcattcaga atcatcagtg gaatcagttt
     8161 ttcaggaaaa tgataagggc cgtagttatt agaacaattg gtcacaatgg tcggcaggcc
     8221 gtaagtacgc agccatgcgc gcaccaaatg gtcgctggag gctttggaag ccgaataagg
     8281 actggaaggg gcgtaaggcg tggtttcggt aaacaaatca tccgtgccgt gcaaatcgcc
     8341 atagacttca tcggtggaaa tatggtggaa acggaaggct tcgcgctgtt cagacggcat
     8401 ttgccgccgg taggcgcgcg cagcttcaag tagattgaac gtgccgatga tattggtttg
     8461 gataaattct cctgcagaat cgatggagcg gtcgacatga ctttccgccg ccaagtgcat
     8521 cacggcatca ggccggtgtt gtgcgaatac gcggtcgagt tcggcgcggt cgcaaatatc
     8581 cacttgttca aaagcatagc ggggattatc ggctacctcg gtcaaagatt tcaaattgcc
     8641 ggcataagtc agcttatcga cattgacgac agcgtcccgg gtgtttcgga taatatggcg
     8701 gacaacggca gaaccgataa agcccgcgcc gccggtaacg aggatggttt ttttgtttgc
     8761 ggtttgcatt taatccccat atccgttggg gttgcggctg acccaacgcc acgaatcctc
     8821 catcatttgt ttcaaaccgc gtttggtttc ccagccggtt tgttgtttgg tatgggacgg
     8881 gtcggcaaag gaacacgcca agtcgccggc gcgacggggt tggatttgat agggaatatt
     8941 taagccggat gcggcttcaa aggcacggat gatttccaaa acggaatagg cgcgccccga
     9001 acccaagttg aacaaatgta cgccggcaac accgcctttc gccttcattg ccgcgatatg
     9061 cccttctgcc aaatccatca catgaatata gtcgcgcata ccggtgccgt cgggggtcgg
     9121 ataatcgccg ccgaataccg acagttgcgg cagcctgccc gaagccactt ggcagatata
     9181 gggcaaaagg ttgttgggaa tgccgttggg ctgttcgccg atcaagccgc tttcatgcgc
     9241 gccgatcggg ttgaaataac gcaacaaaat cacgctccaa cgcggatcgg ctttttggat
     9301 gtcggttaac atccgttcca ccatcgcttt ggacgcgcca taaggattag cggtatcgcc
     9361 cgggcgcata tcttccgtat agggcatttt acgtatcatg gcgcgacgca ttaaagtaaa
     9421 cgccaaagcg cgtttatact ggttatccag tttccctata tttttacggt acaaatcaag
     9481 ttgttggcat tcatcgtgaa aatagaattt ttcggcataa cgctgactca taaaaccggc
     9541 atgcgggctg cctgaaaaaa tcattgcgac atcatcgtca tttaatgttt catggttgtt
     9601 ttctattaat gctaaagcaa tttggtaatt aattgccaaa atatcgttag taatcacacg
     9661 atagccgcgt tttttggctt cataggcaaa ggaacagcct ccactaaata catctgcaac
     9721 tgtatctaca tcagacggag gctggtcaca aatccaggaa gctattttct ctttattacc
     9781 gatatagtta attttcggat attgcttatt cattttcact catttttaaa atactttcag
     9841 caatagcttt tgccaataaa ggcggtacgg cgttaccaac ctgctgctgc tgggcaattt
     9901 tgctgccgca aaaaataaaa ttatcaggga aagattgtaa ggcagctaat tcacgaacgg
     9961 ttaacgcccg attctgttca tagtgaaaaa ctttgcgcat atctcctgta atacaaacgg
    10021 ctggttttgt tgctgttgta acggatgtat ttacggatat cacctgtttt cggacgtaat
    10081 ggttcaggaa tatcgttacg gttacctcca tttttaacaa atgccatttt ttctaacatt
    10141 tgtgccgaat gattcatagc ttcatgattt gcaacgtgtg gattgctttc gccagcagcc
    10201 agttttggaa aatgtcctat tgctgatcca acagtctaat gggaaatctg caaaggttcg
    10261 ggaaaggaaa ttttgccttt atccctcctc ccgataaata tcactcggct acgtatctga
    10321 ggaacaccga aatcggctgc actcagtatc ttacattcca ccgaataacc gatattctga
    10381 aatgcttgaa taatctcaat acgtgtttta cctgaattgt gtgtatagag tcgcgctaca
    10441 ttttccataa caaaaaaata tggttggaca attttaacta ttcggacaaa ctctttaaat
    10501 aaatggttgc gtgggtcatc tgtaaatgtc cgtccaatct ttcctgccat actaaaacct
    10561 tgacaaggtg gtcctccaat aatcaaatca actgcttgtc cgttaagaca attgattaaa
    10621 tcttgttcgg ttagtgtggt taaatctttt tgcagtaatt gatgatgggg gaagttggta
    10681 cggtaagtct gacaataatc agactccatt tcaacagaaa gcaattgttg gaatccggct
    10741 tgttcaaaac ccaaggatag gcctcctgct cctgagaaaa ggtcaatata ggtaagtggt
    10801 gtgtgcataa gtcaaaatcc ataaactctt cgattatttt accgcttcag cttcgcaaac
    10861 gcatccgcca tcgccgaatt ggctggggcg cggtcgttgc gttggggttt gcggccggct
    10921 gtccgttcgc ggctgcggtt ttcagacggc attttatgtt ttgcgccgcc cggttcgtca
    10981 tccaagcgca tggtcagcgc gatgcgtttg cgtgcagcat cgacttccag cactttcact
    11041 ttcaccacgt cgccagcttt caccacttcg cgcgggtctt ggacgaactt gttggacagg
    11101 gcggagatgt gcaccaagcc gtcctgatgg acgccgatgt ccacgaacgc gccgaagttg
    11161 gcgacgttgg aaaccacgcc ttcgagtatc ataccgactt gcaagtcgct gatttcgtgg
    11221 ataccttcgg caaacgatgc cgtctgaaac tcgccgcgcg gatcacggcc gggtttttcc
    11281 agttcggaca ggatgtccaa aatggtcggc aggccgaagc gttcgtcggt gaagtcggac
    11341 gctttgattt gcttcacgcg ctcgcggttg ccgatgagtt cggcggcgtt aatgccttgt
    11401 tgcgccagca ttttggcgac gacgggatag gcttcggggt ggacggcgct cgcgtccaac
    11461 ggctctttac cgccgttaat ccgcaaaaag cctgccgcct gctcgaaggt tttttcgccc
    11521 aaacgcggta ctttcagcaa ttttttgcgg ctgtcgaacg cgccgttttc atcgcggtag
    11581 gcaacgatgt tttgggcaag ggtctgattc aaaccggaaa tccgcgccaa gagcggggcg
    11641 gaggcggtgt tcacgtccac gccgacggcg ttcacgcagt cttcgaccac cgcgtccagc
    11701 gatttggcga gccggctttg gttcacgtcg tgctgatact gccccacgcc gatggatttg
    11761 gggtcgattt tgaccaactc ggcaagcggg tcttgtagcc tgcgggcgat ggacaccgcg
    11821 ccgcgcaggg aaacgtccaa gtcggggaac tcgcgcgccg ccagttcgga cgcggaataa
    11881 atcgacgcgc cggcttcgga cacgacgatt ttatgcagtc ccatttccgg cattccacgc
    11941 accagttcgc ccgcgatttt gtcggtttca cggctggcgg tgccgttacc gatggcgatg
    12001 agcttcacgc cgtgctgttt aatcaggcgc gacagcgttg ccaacatatt gttttcttga
    12061 tgcaaataga cgatgacggt atccagcagc ttgccggtgt cgtccaccac ggcgcatttc
    12121 acgccgttgc ggtagccggg gtcgagaccc agcgtggtca gccgtccggc gggcgcagca
    12181 agcagcaagt ctttgagatt gcgggcgaac acggtaatcg cgtcggtgtc ggcggcttct
    12241 ttcaaacgat tgagagcttc aagttccaac gacaaaaaga ttttcgcgcg ccaagtcagg
    12301 cgcacggtgt cgcgcagcca tttgtggccg tctgaaactt tgaagcggcg ggcgatgatt
    12361 tgctcgtatt cgctttgctg cgtaatcggc gtgtcgtcgg gctgatattt gagcgcgatg
    12421 ttcaacacgc cttcgttgcg tccgcgcaaa accgctagcg cacgatggct gggcatcgca
    12481 cgaataggtt cgcggtggtc aaaataatcg ctgaattttt cgccttcggt ttctttgcct
    12541 ccaacaactt gcgcgtggat ttcggcttcg ttccacagct tgtcgcgcag cgtgccgatg
    12601 agttccgcgt cttcggcaaa ctgctccatc agaatcgcac gcgcgccgtc caacgcggct
    12661 ttggcatcgg ggacgttttc gttcaggtag ccttgtgcgg cggcttccac gtcctgcgac
    12721 tgctcggcaa gcaacacgtc cgccagcggc tgcaaaccgt gttcgcgcgc gatttgcgct
    12781 ttggtgcggc gtttgggttt gtagggcaga tacaggtctt ccagcgcggt tttgttatcg
    12841 gcggcttcga tttgcgccct gaggtcgtct gaaagcttgc cttgctcttc aatgcttttt
    12901 aaaacaacgg ctttgcgctc ttccaactcg cgcagatatt gcagccgctc ggcaagccgg
    12961 cgcaactgcg tatcgtccaa cccgcccgtc gcttccttgc ggtagcgggc gataaacggc
    13021 acggtcgcgc cgtcgtccaa aagctcgacg gcggcggtga tttgcgcggc agtcgcggag
    13081 agttcttggg aaagaatttg agtaatgttc atcaatagaa ttccaacgga caggccgtct
    13141 gaaatttcag acggcctgat ttaaaaacaa tcgctttaag gcagcgaatt ataatattcg
    13201 taggctttgt ccatatcttc aaactggtac atatgccctt tttccagcac catcgcatta
    13261 tcgcaatatt gcttcatggc gctgtggctg tgcgacacca agatgatgga acggtctttg
    13321 cgcttttcaa acaactcgta cttacattta tcggcaaaac gcgagtcacc aactgcaatc
    13381 acttcgtcaa tcaaatagca atcaaactct accgccaacg acaaagcaaa tgccaaacgc
    13441 gccttcatac ctgaagaata acgcttgacc ggctcgtaaa gatattgccc caactcggaa
    13501 aactcttcgg taaagttctt cacataatcg atatcgacat tgtaaatccg gcagatgaaa
    13561 cgcaaattgt ccataccggt cagactgcct tgaaacgcac cggagaatgc caaaggccaa
    13621 gaaatactca ttgtccgctt gatttcaccc gtggtcggcg gctcaacgcc actgatcaaa
    13681 cggatgagcg tcgatttacc tgcaccgttg cggccgagaa taccgatttt ctcgcccttc
    13741 tccattttga agctaatatc gtgcaagact gtccgccaac cttggcgggt cagatagcgt
    13801 ttggaaacgt gttcaactga aatcattgcg gctcgactcc tttactgaat ttactgacca
    13861 tcgccaagcc aaacaacaac aacaccagat tgcacaatac gatataccaa ggattttcat
    13921 aggtaattac atcgctgcca aaatatccgg cacggaacat ttctgtgcca tgcaccatcg
    13981 ggatcatcaa tgcatactct tgcactttcg gaggcaaatt atgtacaaaa aagaatgcac
    14041 ccgataacgg catcatcaca aaagtcaatg tgccccaaat cttgccaaac ggctcgaaat
    14101 taaaggcaat cgaacaaatc accaaaccca aaccaatcgc aaaaaaagcc atcaaaagcc
    14161 aagccatcag catataaaac atatctgccg gcatttcaat ccagccaatc gcaatcaata
    14221 ccgccataat cacaatctgc gcaatggttg caccagcaat ttccaaaatc atgcgcgcca
    14281 agatggtatc caaaactctt acattgcggt gataaagcaa gctggcattt gaagaaatcg
    14341 acccaactgc ccgttttgag gcattacgcc acatcatcaa catcggatag ccagtaatcg
    14401 caaatgcgac aatattcaaa gttgaatatc ggtctgcctt taaaaatttc cacatcaaga
    14461 cgataacgaa tgtcatcagc aacggctcaa caaacagcca taaaaagcca atattattgc
    14521 gaccgtaacg ggtgataatt tcccgcatca aaagcgcacc gattacgcgc ctttgaatgg
    14581 ctaaagattc ccaaaatgat gttttatgca aggctttcat cagtttttat gctcacgaat
    14641 gctggcagtc aacaggctca aaataccata aaccatcaag ccgataatca gagtggcaac
    14701 aatgttgtat aaccgtttag gctcatgtgc caaatccggc aggctcggtt gcgagatcac
    14761 ttccaaataa agctgctgac ggtctgcttc aaccttggca ctttccaaag aagtcatggc
    14821 ggctgccaac tgctgctctg ccaactggtt ttccaaatac acacgctgat attcggcagc
    14881 ctgattagac aacgaagaat gcccaccgcc cgaaatggca cgtaactgtt ggtcaatttc
    14941 tttacgcaag ctctgctcac gcgcctgcaa acccggaatc tgcggattct ccggagtgac
    15001 tgctttcacc tgatccagct gggtttgaat cacaatcaat tcatcttgca gcttggaaac
    15061 caaccccatt tgcacttccg attgcgcttt caaatcaaaa acgccattgg caatccggta
    15121 atccgtcaga ttctgagagg cttcctttac ccgctctgcc gccgttttca ctacttcttc
    15181 cgcatagcgc accgtatcag cacgtgcacg atcgttcaac tggttaatca atgcttcacc
    15241 ttgttttaac aaagcctcat tgattttctt agattccagc gcatcaaagg aagttacatt
    15301 caacgtggaa atacccgaaa ccgtatcaaa attgatcatc acctgatttt tatagtattg
    15361 ataaaaagcc tcttcctcgc cacggaaccc aaacccatta aagcggctga acgcatcacc
    15421 tttggtttca taaaactcac gcaccggcaa gattttacgc agttcatcca aagacgagcg
    15481 cgaacgcata tactccccaa ccgtgtaaat atcatcttgc gcacgggcaa aacctgtgcc
    15541 ctgcaaaatg gcacccaggc cattgagaga agattggctt ttaggcgagc gcaccacaaa
    15601 gctcgattgc gacgtaaaac gatcggaagc gaagaagccg aaatacacca acgaaattac
    15661 cgtagggata atcaccgtta cccaaaataa agggcttagc tttttaatcc aacttttctt
    15721 tttcggcttt ttacgctcgg ctttggtttc agtggcaact gccacaggaa gttgctcaga
    15781 catcttaatt actcacatta attagttaaa ttattaatac tgttcgcgcc actggtaacc
    15841 ggcgagaaca caaacgacaa aaatttctgc acttcagcca acggcgcatt cgacacatac
    15901 aatacatctt tattcttcac aggaaagcgc tgcatagaaa atagcgaatg cgcatcagcc
    15961 atattcacac gatataccgt tggaatctct gcctcactgc cataaccttg agcaatccat
    16021 ttatcctgac gttctgccgg caattccacc aatggcgtat agcggaacac aaacacacca
    16081 cgcgcatcag aacggcgatc ttgcaaaccg cccatacggc caatggcttc agaaagcgat
    16141 aagcctctgg ctgaaaaacc gatttcttgt gttctcccca ccgcacccat agacgtaaag
    16201 gtatagggat tggtaatcat ggtaaccaca tcaccgcgac gcagcaaaat attttgtcgc
    16261 ggatttgcaa ctaaatcttc caaggcaaca gttcgtacta cattgccacg tgtcagctgc
    16321 acattcgtat cctgcacatt tgccgttgaa ccacctaccg cagccaccgc atccaacaca
    16381 cgctcaccgg ctgccgtcag cggcatacgc acactattcc cagcacgaat caccgacaca
    16441 ttcgccgcat tattctgcac caaacgcacc atcacttgtg gctgattggc catttttttc
    16501 aggcggcctt taataatttc ctgaacctga ccaggcgttt taccgaccac cgaaatatcg
    16561 ccaacaaacg gcacagaaac cgtaccacgt gccgtgacca actgctctgg caacttagtt
    16621 tgatgcgcac tacccgagcc catcgaagaa aggccaccac caaacaatac tgccggcggc
    16681 gcttcccaaa tcataatatc caatacatca ccaatattta gcgtaccagc cgaagcataa
    16741 ccatcgccaa actgagtgaa tgactgattt atctgagcct tatataataa ctgagcaacc
    16801 gtatgattca catcaatcag ctccacttca ggaatttgaa cttcagattg ttgccctaaa
    16861 gagacaattt tttttgcgct ggggcctgat gaaggaatcg cagagcatcc tacaattaaa
    16921 cttccacaca ataataatac tgcgtgacga atataaaatt tcactttaaa cacaagccaa
    16981 atcctaatat aattataaat ggcctaatta tagcacttaa tcgaaataaa tttatgagta
    17041 cgtagagtat aattagtatt cttctttcca acttccttat acttatatac ttatagattc
    17101 taaaatcatg aaaagaattc tttgcattac aggtaccaga gccgacttcg gcaagctaaa
    17161 acctttatta gcctatattg aaaatcaccc agaccttgaa ttgcatttga ttgtaactgg
    17221 tatgcatatg atgaaaacat atggcagaac ctacaaggaa gtaactcgag aaaactatca
    17281 acatacatat ctgttttcaa atcaaatcca aggtgaacca atgggtgccg ttttaggcaa
    17341 taccattacg tttatctctc gtctatctga tgaaattgaa cctgatatgg tcatgattca
    17401 cggcgaccgt ttagaagcac tagcaggcgc agctgtaggt gcattaagca gccgtttagt
    17461 ttgccatatc gaaggtggtg aactatctgg tacagtagat gactccattc gtcattctat
    17521 tagtaaactt tctcatatcc acttggtagc aaatgaacaa gctgtcactc gcctagtgca
    17581 aatgggagaa aaaagaaagc atattcacat catcggctcc cccgatttag atgttatggc
    17641 ctcttccacc ctcccatcct tagaagaagt caaagaatat tacggtttac catacgaaaa
    17701 ttatggtatt tctatgtttc accccgtgac tacagaagca catttaatgc cacaatatgc
    17761 ggcccaatat ttcaaagcat tagaattaag tggccaaaat atcattagca tctaccctaa
    17821 taatgacacg ggcactgaaa gtattctgca agaattatta aaatatcaat ctgataaatt
    17881 tattgcattt ccttccattc gatttgaata ttttttagtc ttattgaaac atgctaaatt
    17941 tatggtcggc aactcaagtg caggtattag agaagctcct ctctacggtg tcccttcaat
    18001 tgatgttggt acacgccaaa gtaaccgcca tatgggaaaa tctattattc atacagatta
    18061 tgaaactaaa aatatctttg atgcgattca acaagcatgc agtttaggca aatttgaagc
    18121 agatgatacc tttaatggcg gagatactcg caccagcaca gaaagatttg ctgaagtaat
    18181 caacaatcct gaaacgtgga atgtttctgc tcaaaaacgt tttatcgatt tgaatcttta
    18241 aattatggaa aaacaaaata ttgcggttat acttgcgcgc caaaactcca aaggattgcc
    18301 attaaaaaat ctccggaaaa tgaatggcat atcattactt ggtcatacaa ttaatgctgc
    18361 tatatcatca aagtgttttg accgcataat tgtttcgact gatggcgggt taattgcaga
    18421 agaagctaaa aatttcggtg tcgaagtcgt cctacgccct gcagagctgg cctccgatac
    18481 agccagctct atttcaggtg taatacatgc tttagaaaca attggcagta attccggcac
    18541 agtaacccta ttacaaccaa ccagtccatt acgcacaggg gctcatattc gtgaagcttt
    18601 ttctctattt gatgagaaaa taaaaggatc cgttgtctct gcatgcccaa tggagcatca
    18661 tccactaaaa accctgcttc aaatcaataa tggcgaatat gcccccatgc gccatctaag
    18721 cgatttggag cagcctcgcc aacaattacc tcaggcattt aggcctaatg gtgcaattta
    18781 cattaatgat actgcttcac taattgcaaa taattgtttt tttatcgccc caaccaaact
    18841 ttatattatg tctcatcaag actctatcga tattgatact gagcttgatt tacaacaggc
    18901 agaaaacatt cttaatcaca aggaaagcta aatgcaaaac aacaacgaat ttaaaattgg
    18961 taatcgttca gtaggttaca accacgaacc attgattatc tgtgaaatcg gcatcaatca
    19021 tgaaggctct ttaaaaacag cttttgaaat ggttgatgct gcctataatg caggcgctga
    19081 agttgttaaa catcaaacac acatcgttga agacgaaatg tctgatgagg ccaaacaagt
    19141 cattccaggc aatgcagatg tctctattta tgaaattatg gaacgttgcg ccctgaatga
    19201 agaagatgag attaaattaa aagaatacgt agagagtaag ggtatgattt ttatcagtac
    19261 tcctttctct cgtgcagctg ctttacgatt acaacgtatg gatattccag catataaaat
    19321 cggctctggc gaatgtaata actacccatt aattaaactg gtggcctctt ttggtaagcc
    19381 tattattctc tctaccggca tgaattctat tgaaagcatc aaaaagtcgg tagaaattat
    19441 tcgagaagca ggggtacctt atgctttgct tcactgtacc aacatctacc caacccctta
    19501 cgaagatgtt cgattgggtg gtatgaacga tttatctgaa gcctttccag acgcaatcat
    19561 tggcctgtct gaccatacct tagataacta tgcttgctta ggagcagtag ctttaggcgg
    19621 ttcgatttta gagcgtcact ttactgaccg catggatcgc ccaggtccgg atattgtatg
    19681 ctctatgaat ccggatactt ttaaagagct caagcaaggc gctcatgctt taaaattggc
    19741 acgcggcggc aaaaaagaca cgattatcgc gggggaaaag ccaactaaag atttcgcctt
    19801 tgcatctgtc gtagcagata aagacattaa aaaaggagaa ctgttgtccg gagataacct
    19861 atgggttaaa cgcccaggca atggagactt cagcgtcaac gaatatgaaa cattatttgg
    19921 taaggtcgct gcttgcaata ttcgcaaagg tgctcaaatc aaaaaaactg atattgaata
    19981 atgcttatta acttagttac tttattaaca gaggattggc tattacatat agctaattct
    20041 cattaatttt taagagatac aataatgcta aagaaaataa aaaaagctct ttttcagcct
    20101 aaaaagtttt ttcaagattc aatgtggttg acaacatctc cattttatct taccccccca
    20161 cgtaacaatt tatttgtcat atctaattta ggtcagctta accaagtcca aagcctaatt
    20221 aaaatacaaa aattaaccaa taatttacta gtaattttat atacttctaa aaacttaaaa
    20281 atgcctaagt tagttcatca atcagctaac aagaatctat ttgaatctat ttatctattt
    20341 gagcttccta gaagccctaa taatataact cctaaaaaat tactttatat ttatagaagt
    20401 tacaaaaaaa tccttaatat tatacagcct gctcatctct atatgctgtc ttttacaggc
    20461 cactactcct atctgattag tattgcaaaa aaaaagaata ttacgactca tttaattgat
    20521 gaagggactg gaacatatgc tcctttatta gaatcatttt catatcatcc aacaaaatta
    20581 gaacgttatt tgattggaaa taatcttaat attaaaggat atatagatca ttttgacata
    20641 ttgcatgtcc cctttcctga atatgctaaa aaaatattta atgcaaaaaa atataaccgg
    20701 ttttttgcgc atgctggagg aataagcatt aataataaca ttgcaaactt acagaaaaaa
    20761 tatcaaatat ctaaaaatga ctatattttt gttagtcaac gctaccccat ttcagatgat
    20821 ttgtattata agagtatagt agaaatctta aacagcataa gtttacaaat taaaggaaag
    20881 atatttatta aactacaccc aaaagagatg ggcaacaact atgtaatgtc tttatttcta
    20941 aatatggtag aaataaaccc tcggctggta gttattaatg aacctccttt tctaattgag
    21001 cccctaatat acttaacaaa tcctaaagga attataggcc tggcctctag ttctttaatt
    21061 tatacaccat tactctcacc ctcaacccaa tgtctttcta ttggagagtt aattattaac
    21121 ttaattcaaa aatattcaat ggtggaaaac actgaaatga tccaagaaca cttagagatt
    21181 attaagaaat ttaattttat taatatacta aatgatttaa atggggtaat aagtaaccct
    21241 ctctttaaaa cagaagaaac atttgaaaca cttcttaaat ctgcagaatt cgcatataaa
    21301 tctaaaaact actttcaggc tattttttac tggcaacttg ccagcaaaaa caatattacc
    21361 ttattagggc ataaagcatt atggtactac aatgcacttt ataatgtaaa acaaatttat
    21421 aagatggaat attcagatat tttttatatc gataatatct ccgtagactt tcatagtaaa
    21481 gataaattga catgggaaaa aattaaacat tattactatt ccgccgacaa tagaattggt
    21541 agagatagat aaaaatacta tgacttctga tctccatacg tatgcttgtt ttcaaaaagc
    21601 agcattaaga tagataagtg aagcttgatg agagccattt ttgtggtcat cgccaaggta
    21661 cacgcggaga gtaatcgaca acagagctgc tgtattctgg cttttaaagc ccagtagtaa
    21721 agtttataag gctgagacct ttgcaaaat
//
LOCUS       DAWHSK010000009        21564 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.009, whole genome shotgun sequence.
ACCESSION   DAWHSK010000009 DAWHSK010000000
VERSION     DAWHSK010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21564
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.009"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..149)
                     /locus_tag="WIC03_000253"
     CDS             complement(<1..149)
                     /locus_tag="WIC03_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEZ3882815.1"
                     /translation="MTSTFPRRLARKIRQTRRLSRKSIAFLFLLAGSALVALTALFFA
                     HLADFA"
     gene            complement(209..1429)
                     /gene="argJ"
                     /locus_tag="WIC03_000254"
     CDS             complement(209..1429)
                     /gene="argJ"
                     /locus_tag="WIC03_000254"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEZ3882816.1"
                     /translation="MAVNLTEKTAEQLPDIDGIALYTAQAGVKKPGHTDLTLIAVAAG
                     STVGAVFTTNRFCAAPVHIAKSHLFDEDGVRALIINTGNANAGTGAQGRIDALAVCAA
                     AARQIGCKPNQVLPFSTGVILEPLPADKIIAALPKMQPAFWNEAARAIMTTDTVPKAA
                     SREGKVGDKHTVRATGIAKGSGMIHPNMATMLSFIATDAKVSQPVLQLMTQEIADETF
                     NTITVDGDTSTNDSFVIIATGKNSQSEIDNIADPRYAQLKELLCSLALELAQAIVRDG
                     EGATKFITVRVENAKTRDEARQAAYAVARSPLVKTAFFASDPNLGRLLAAIGYAGVAD
                     LDTDLVEMYLDDILVAEHGGRAASYTEAQGQAVMSKAEITVRIKLHRGQAAATVYTCD
                     LSHGYVSINADYRS"
     gene            complement(1496..2188)
                     /locus_tag="WIC03_000255"
     CDS             complement(1496..2188)
                     /locus_tag="WIC03_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEZ3882817.1"
                     /translation="MNEILRQPSVLLFLTLAVYALAIIVRTRTGNIFCNPVLVSTIVL
                     IAYLKILDIDYAVYHNAAQFIDFWLKPAVVVLAVPLYQNRRKIFNQWLPVIVSQLAGS
                     VTGIVTGMYFAKWLGAEREVVLSLASKSVTNPIAIEITRSIGGIPAITAATVIIAGLV
                     GQIAGYKMLKNTVVMPSSVGMSLGTASHAMGIAASLERSRRMAAYAGLGLTFNGVLTA
                     LIAPLLIPVLGF"
     gene            complement(2188..2532)
                     /locus_tag="WIC03_000256"
     CDS             complement(2188..2532)
                     /locus_tag="WIC03_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEZ3882818.1"
                     /translation="MNIIRALLIILGCLAAGETAVFLAGIKLPGSIVGMGVLFALLQA
                     GWVKTSWLQQLTDALMANLTLFLVPPCVAVISYLDLIADDWFSILVSASASTLCVLLV
                     TGKVHRWIRSII"
     gene            complement(2677..2895)
                     /locus_tag="WIC03_000257"
     CDS             complement(2677..2895)
                     /locus_tag="WIC03_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3882819.1"
                     /translation="MSMPEIPKWYDDDGQIVSCTEKIKVMSENMAELYQTAQDAFEDA
                     LLMGCGERQLRDYLLALIEGLENPYRKV"
     gene            complement(2914..3495)
                     /locus_tag="WIC03_000258"
     CDS             complement(2914..3495)
                     /locus_tag="WIC03_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEZ3882820.1"
                     /translation="MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAED
                     EQPVLPINRAPARRAGNADELIGNAMGLLGIAYRYGGTSVSTGFDCSGFMQHIFKRAM
                     GINLPRTSAEQARMGTPVARSELQPGDMVFFRTLGGSRISHVGLYIGNNRFIHAPRTG
                     KNIEITSLSHKYWSGKYAFARRVKKNDPSRFLN"
     gene            3740..4648
                     /gene="hslO"
                     /locus_tag="WIC03_000259"
     CDS             3740..4648
                     /gene="hslO"
                     /locus_tag="WIC03_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEZ3882821.1"
                     /translation="MNQTAINRADVRTRFIFDDMPVRGLHVRLENVWQHIVKQKNYPA
                     AIRRALGELLAAGVLLSGNLKNEGTLIVQVQGQGRLKMLVAEATSDRTVRATARWDEA
                     AEIADDESLGDLLGGNGVFVLTLQPKDGEPWQGVVPLEGDGIAQMLMNYMKRSEQLDT
                     HIVLSASDEAAGGLLVQRLPEEVLDEEAWEHVSTLARTLTAGELAELDAQHVLYRLFH
                     ETPPRVFEPETFEFSCTCSRGKVSDMLLMLGGEEVGGVVAEQGSIEVDCDFCHSKYVF
                     DETDVNALFGEDVVGVAKGLPRHTVQ"
     gene            complement(4734..6188)
                     /gene="mgtE"
                     /locus_tag="WIC03_000260"
     CDS             complement(4734..6188)
                     /gene="mgtE"
                     /locus_tag="WIC03_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEZ3882822.1"
                     /translation="MSIEPTPPNLENDGIENDVERVSADFDRVHSLCEILEPAFEQIE
                     NGTPLEDAPLRDKLTELTVLLSELHPADVAAVLESLPPRERNIVWILVKPEDDGEVLL
                     EVSDAVRETLIESMDKDELLAAVDDLDADELAELADDLPHQVVYEALQTRDEEERAQV
                     KAAMSYEDNQVGAIMDFELVSIRADVACEVVLRYLRRFDSLPDHTDKIFVVDENDVLQ
                     GVLPIRKLLVADPEDLVENVMAKDVVRFRAEDDVEEAAQAFERYDLVTAPVVDENKKL
                     IGRITVDEMVDVIREESEADMLNMAGLQEEEDLFAPVLDSVKNRWMWLAVNLCTAFLA
                     SRVIGAFEGSIEKIVALAALMPIVAGIGGNSGNQTITMIVRAMAMGQLTGMQAGRLLK
                     KEVGVALVNGIIWGTVMGAVSWLLYGSLGIGLVMIAAMTLNLLLAATVGVLIPVVMEK
                     FGRDPALGSSVLITAVTDSGGFLIFLGLATLFLL"
     gene            complement(6420..10895)
                     /gene="mspA"
                     /locus_tag="WIC03_000261"
                     /gene_synonym="ausI"
     CDS             complement(6420..10895)
                     /gene="mspA"
                     /locus_tag="WIC03_000261"
                     /gene_synonym="ausI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin/serine protease MspA"
                     /protein_id="HEZ3882823.1"
                     /translation="MRFTHTTPFCSVLSTLGLFAVSPAYSSIVRNDVDYQYFRDFAEN
                     KGAFAVGASNISIQDKQGKILGRVLNGIPMPDFRVSNRQTAIATLIHPQYVNSVKHNV
                     GYGSIQFGNDTQNPEEQAYTYRLVSRNPHPDYDYHLPRLNKLVTEISPAALSSVPLLG
                     NGQPKANAYLDTDRFPYFVRLGSGTQQVRKADGTRTRTAPAYQYLTGGTPLKVLGFQN
                     HGLLVGGSLTDQPLNTYAIAGDSGSPLFAFDKHENRWVLAGVLSTYAGFDNFFNKYIV
                     TQPEFIRSTIRQYETRLDVGLTTNELIWRDNGNGDSTLQGLNERITLPIANPSLAPQN
                     DSRHMPSEDAGKTLILSSRFDNKTLMLADNINQGAGALQFDSNFTVVGKNHTWQGAGV
                     IVADGKRVFWQVSNPKGDRLSKLGAGTLIANGQGINQGDISIGEGTVVLAQKAASDGS
                     KQAFNQVGITSGRGTAVLADSQQIKPENLYFGFRGGRLDLNGNNLAFTHIRHADGGAQ
                     IVNHNPDQAATLTLTGNPVLSPEHVEWVQWGNRPQGNAAVYEYINPHRNRRTDYFILK
                     PGGNPREFFPLNMENSTSWQFIGNNRQQAAKQVAQAENARPDLITFGGYLGENAQTGK
                     AAPSYSKTNIEWVNGPNTPHKPSPYTNTPTRTGAGVPTITASKKAATPAPTSHGMPTA
                     PKTGNFSDMTGEAAIEKTRHIANAAVYGRPEYRYNGALNLHYRPKRTDSTLLLNGGMN
                     LNGEVLIEGGNMIVSGRPVPHAYDHQAKREPVLENEWTDGSFKAARFTLRNHARLTAG
                     RNTAHLDGDITAYDLSGIDLGFIQGKTPECYRSYHSGSTHCTPNAVLKAENYRALPAT
                     QVRGDITLNDRSELRLGKAHLYGSIRAGKDTAVRMEADSNWTLSQSSHTGALTLDGAQ
                     ITLNPDFANNTHNNRFNTLTVNGTLDGFGTFRFLTGIVRKQNAPPLKLEGDSRGAFQI
                     HVKNTGQEPQTTESLALVSLNPKHSHQARFTLQNGYADLGAYRYILRKNNNGYSLYNP
                     LKEAELQIEATRAEHERNQQAYNQLQATDISRQVQHDSDATRQALQAWQNSQTELARI
                     DSQVQYLSAQLKQTDPLTGILTRAQNLCAAQGYSVDICRQVAKAADTNDLTLFETELD
                     TYIERVEMAESELDKARQGGDAQAVETARHAYLNALNRLSRQIHSLKTGVAGIRMPNL
                     AELISRSANTAVSEQAAYNTGRQQAGRRIDRHLTDPQQQNIWLETGTQQTDYHSGTHR
                     PYQQTTNYAHISIQTGITDRLSVGTILTDERTNNRFDEGVSARNRSNGAHLFVKGENG
                     ALFAAADLGYSNSRTRFTDYDGAAVRRHAWDAGINTGIKIDTGINLRPYAGIRINRSN
                     GNRYVLDGAEINSPAQIQTTWHAGIRLDKTVELGQAKLTPAFSSDYYDTRQNSGSALS
                     VNDRTLLQQAAHGTLHTLQIDAGYKGWNAKLHAAYGKDSNTARHKQAGIKIGYNW"
     gene            complement(11209..11967)
                     /gene="gloB"
                     /locus_tag="WIC03_000262"
     CDS             complement(11209..11967)
                     /gene="gloB"
                     /locus_tag="WIC03_000262"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HEZ3882824.1"
                     /translation="MKITPVKALTDNYIWMIQHGNHAVCVDPSEPSPVLEFLVRNRLM
                     LAQTWVTHPHPDHEGGAAALWRGYMESPVYGESDIEAATHTVTAGTQFTFGDGQVTVW
                     ATPGHTDRHTSYLLETSDGIHVFCGDTLFSAGCGRVFTGTIEQLYDSFQRFNRLPENT
                     LFYPAHEYTAANLRFAAHIEPDNADIQTALKAAAHTPTLPVTLAHERRVNPFLRVDLP
                     HVRDRAEALSGKTLNSSLDTFVALRELKNQYRTK"
     gene            complement(12062..16048)
                     /gene="purL"
                     /locus_tag="WIC03_000263"
     CDS             complement(12062..16048)
                     /gene="purL"
                     /locus_tag="WIC03_000263"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEZ3882825.1"
                     /translation="MSVVLPLRGVTALSDFRVEKLLQKAAALGLPEVKLSSEFWYFVG
                     SEKALDAATVEKLQALLAAQSVEQTPKAREGLHLFLVTPRLGTISPWASKATNIAENC
                     GLAGIERIERGMAVWLEGALTDEQKQQWAALLHDRMTESVLPDFQTASKLFHHLESET
                     FSTVDVLGGGKEALVKANTEMGLALSADEIDYLVENYQALNRNPSDVELMMFAQANSE
                     HCRHKIFNADFILNGEKQPKSLFGMIRDTHNAHPEGTVVAYKDNSSVIEGAKIERFYP
                     DAAENQGYRFHEEDTHIIMKVETHNHPTAIAPFAGAATGAGGEIRDEGATGKGSRPKA
                     GLTGFTVSNLNIPDLKQPWEQAYGKPDHIASPLDIMIEGPIGGAAFNNEFGRPNLLGY
                     FRTFEEKFDGQVRGYHKPIMIAGGLGSIQAQQTHKDEIPEGALLIQLGGPGMLIGLGG
                     GAASSMDTGTNDASLDFNSVQRGNPEIERRAQEVIDRCWQLGDKNPIISIHDVGAGGL
                     SNAFPELVNDAGRGAVFKLREVPLEEHGLTPLQIWCNESQERYVLSILEKDLDAFRAI
                     CERERCPFAVVGTATGDGHLKVRDDLFSNNPVDLPLNVLLGKPPKTTRTDKTVTPYAT
                     EGNPFGKKPFNAGDIDITEAAYRVLRLPAVAAKNFLITIGDRSVGGMTHRDQMVGKYQ
                     TPVADCAVTMMGFNTYRGEAMSMGEKPAVALFDAPASGRMCIGEAITNIAAVNIGDIG
                     NIKLSANWMAACGNEGEDEKLYRTVEAVSKACQALDLSIPVGKDSLSMKTVWQDGADK
                     KSVVSPLSLIISAFAPVEDVRKTVTPELKNVEDSVLLFIDLGFGKARMGGSAFGQVYN
                     NMSGDAPDLDDTSRLKAFYNVIQQLVAEDKLLAYHDRSDGGLFAVLVEMAFAGRCGLD
                     IDLNLLLAQTFITNHTALSQSLRTEEVKALAEWQETIARTLFNEELGAVIQVRKQDVA
                     DIINLFYQQQLHHNVFEIGTLTDENTLIIRDGQTHLISDNLIKLQQTWQETSHQIQRL
                     RDNPACADSEFALIGDNERSALFADVKFDVNEDIAAPFINSGAKPKIAILREQGVNGQ
                     IEMAAAFTRAGFDAYDVHMSDLMAGRFRLADFKMLAACGGFSYGDVLGAGEGWAKSIL
                     FHPALRDQFAAFFADPDTLTLGVCNGCQMVSNLAEIIPGAEAWPKFKRNLSEQFEARL
                     SMVHVPKSASLILNEMQGSSLPVVVSHGEGRADFALHGGNISADLGIALQYVDGQNQV
                     TQTYPLNPNGSPQGIAGITNADGRVTIMMPHPERVYRAAQMSWKPEGWTELSGWYRLF
                     AGARKALG"
     gene            complement(16206..16418)
                     /locus_tag="WIC03_000264"
     CDS             complement(16206..16418)
                     /locus_tag="WIC03_000264"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3882826.1"
                     /translation="MPETVIPAQAGIPISAFQQYLKVSVNPNFHIPNRAETTAFDLKT
                     YGSSCPPLYFLQLSAYPALGKNQKNR"
     gene            16513..16851
                     /locus_tag="WIC03_000265"
     CDS             16513..16851
                     /locus_tag="WIC03_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEZ3882827.1"
                     /translation="MKKIEAIVKPFKLDDVREALTEIGITGMTVSEVKGFGRQKGHTE
                     IYRGAEYAVDFLPKVKIELVLADDAVERAIDVIVEVARSGKIGDGKIFVLPVEEAIRI
                     RTGERADAAV"
     gene            complement(16910..17668)
                     /locus_tag="WIC03_000266"
     CDS             complement(16910..17668)
                     /locus_tag="WIC03_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ3882828.1"
                     /translation="MITIRNVSYRIGTRPILDNVSLDIPEGGITALVGPNGAGKSTLF
                     SFMARLRPLESGSIAYRGKNLADTPTAELAKTLSILTQENSIMSRITVRDLLMFGRYP
                     YHQGRPTAECRRIVNGAIEEFHLQDFADRYLTELSGGQRQRAMIAMVFCQRTDYVLLD
                     EPLNNLDMYHARSLMQILRRLTDEHKRTTVVVLHDINQAAAYADHVVAMKNGQVAMQG
                     KPDDIFTAANIKTLFDMDVSVLDHQSKKLIVHHI"
     gene            complement(17752..18378)
                     /locus_tag="WIC03_000267"
     CDS             complement(17752..18378)
                     /locus_tag="WIC03_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3882829.1"
                     /translation="MTQERFPSFFNQAPTITVRDPLAAFLGAVENGILTYRYADAVRL
                     CGHSCPTVAGAYLMVIKGLKALYGEELPERGGIEAAMQGARDEGTVGVTASVVQLLTG
                     AAPETGFGGIGMQGRFARRNLLSFGAGEINGTLALRRRDTGKTVAVSLNAALQPFAPQ
                     MRDIMPKAVSGSASAEELERFGQLWQARVKAFLTESADDPQFVIVREV"
     gene            complement(18423..19397)
                     /locus_tag="WIC03_000268"
     CDS             complement(18423..19397)
                     /locus_tag="WIC03_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEZ3882830.1"
                     /translation="MPSEKNIGFMAGSSRPLWVAFALLLVSCILFMTLNVKGDWDFVL
                     HLRLTKLAALLMVAYAVGVSTQLFQTLTNNPILTPSILGFDSLYVFLQTLLVFALGGV
                     GYASLPLTGKFGFELVVMMGGSLLLFYTLIRQGGRDLPRMILIGVIFGILFRSLSSLL
                     SRMIDPEEFTAAQANMFAGFNTVHSELLGIGALILLVSAAVVWRERYRLDVHLLGRDQ
                     AVNLGISYTRNTLWILLWIAVLVATATAVVGPVSFFGLLAASLANHFSPSVRHSVRLP
                     MTVCVGGILLVGGQTVFEHFLGMKAVLSVVVEFAGGLVFLYLVLKHKK"
     gene            complement(19387..20355)
                     /locus_tag="WIC03_000269"
     CDS             complement(19387..20355)
                     /locus_tag="WIC03_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEZ3882831.1"
                     /translation="MTAKPFSLNLTNLLLLAVLFAVSLSVGVADFRWSDVFSLSDSQQ
                     VMFISRLPRTFAIVLTGASMAVAGMIMQILMRNRFVEPSMVGASQSAALGLLLMTLLL
                     PAAPLPAKMSVAAVAALIGMLVFMLLIRRLPPTAQLMVPLVGIIFGGVIEAVATFIAY
                     ENEMLQMLGVWQQGDFSSVLLGRYELLWITGGLAVFAYLIADRLTILGLGETVSVNLG
                     LNRTAVLWSGLIIVALITSLVIVTVGNIPFIGLVVPNIISRLIGDRLRQSLPAVALLG
                     ASLVLLCDILGRVIVFPFEIPVSTVFGVLGTALFLWLLLRKPAYAV"
     gene            complement(20519..21484)
                     /locus_tag="WIC03_000270"
     CDS             complement(20519..21484)
                     /locus_tag="WIC03_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ3882832.1"
                     /translation="MLRLTALAVCTALALGACSPQNSDSAPQAKEQAVSAAQTEGASV
                     TVKTARGDVQIPQNPERIAVYDLGMLDTLSKLGVKTGLSVDKNRLPYLEEYFKTTKPA
                     GTLFEPDYETLNAYKPQLIIIGSRAAKAFDKLNEIAPTIEMTADTANLKESAKERIDA
                     LAQIFGKQAEADKLKAEIDASFEAAKTAAQGKGKGLVILVNGGKMSAFGPSSRLGGWL
                     HKDIGVPAVDESIKEGSHGQPISFEYLKEKNPDWLFVLDRSAAIGEEGQAAKDVLDNP
                     LVAETTAWKKGQVVYLVPETYLAAGGAQELLNASKQVADAFNAAK"
BASE COUNT         4361 a         5412 c         6689 g         5102 t
ORIGIN      
        1 gcaaaatcgg caagatgggc aaaaaacagc gcggtcaggg cgacgagtgc cgaacctgcc
       61 aaaaggaaca gaaaggcgat gcttttgcgc gacaggcggc gggtttggcg gattttgcgg
      121 gcgaggcggc gggggaatgt ggaggtcatg gtatgttgtc cggcggttca gacggcattt
      181 tatgtatgcc gtctgaagcc gtgtcgggtc aggaacggta gtcggcgttg atggaaacgt
      241 atccgtgcga caggtcgcag gtatagacgg tggcggcggc ttgtccgcga tgcagcttga
      301 tgcggacggt gatttcggcc ttcgacatca ccgcctgccc ttgtgcttcg gtgtagcttg
      361 cggcgcgtcc accgtgttcg gcaaccaaaa tatcgtcgag atacatttct acgaggtcgg
      421 tatcgaggtc ggcaacgccg gcataaccga tggcggcgag cagcctgccg aggttggggt
      481 cggaggcgaa aaaggcggtt ttgaccagcg gcgaacgtgc cacggcgtag gcggcttggc
      541 gggcttcgtc gcgggttttg gcgttttcga cgcggacggt gatgaacttg gtcgcacctt
      601 cgccgtcgcg gacgatggct tgggcgagtt cgagcgcgag gctgcacaac aattctttga
      661 gttgggcgta acgcgggtcg gcgatgttgt cgatttcgct ttggctgttt ttgccggtgg
      721 cgatgatgac gaagctgtcg ttggtgctgg tgtcgccgtc aacggtgatg gtgttgaagg
      781 tttcgtcggc gatttcctgc gtcatcagtt ggaggacggg ttgggaaact ttggcatcgg
      841 tggcgatgaa actgagcatg gtcgccatat tgggatgaat catgcccgag cctttggcga
      901 tgcccgtggc gcggacggtg tgtttgtcgc cgaccttgcc ttcgcgcgag gcggctttgg
      961 gcacggtgtc ggtggtcatg atggcgcgtg ccgcttcgtt ccagaaggca ggctgcattt
     1021 tgggcagcgc ggcgatgatt ttgtctgcgg gcagcggttc gagaatcacg ccggtggaga
     1081 agggcagcac ttggttcggt ttgcagccga tttgccgggc ggcggcggca cacactgcca
     1141 aagcatcgat tctgccctgt gcgcccgtac ccgcgttggc gttgcccgtg ttgatgatga
     1201 gggcgcgcac gccgtcttcg tcgaaaaggt gcgatttggc gatgtggacg ggcgcggcac
     1261 agaaacggtt ggtcgtgaag actgcaccga cggtgctgcc ggcggctacg gcaatcagtg
     1321 tcaggtcggt atgcccgggc ttcttcacgc ctgcttgggc ggtgtagagg gcaatgccgt
     1381 cgatgtcggg cagttgttcg gcggtttttt cagtcaggtt gacagccata acggttctcc
     1441 ttgcgagtgt gtcggcgttc agacagcatg ggctgaaatg ccgtctgaaa cgggttcaga
     1501 accccaaaac ggggataagc agcggcgcaa tcagcgcggt cagtacgccg ttgaacgtca
     1561 gccccagccc cgcgtatgcc gccatgcggc ggctgcgttc gagcgaggcg gcaatgccca
     1621 tcgcgtgcga agccgtgccg agcgacatac ccacggacga gggcatgacg actgtgttct
     1681 tcagcatttt gtaaccggca atctgtccga ccagaccggc aatgatgacg gtggcggcgg
     1741 taatggcggg aatgccgccg atggagcggg taatttcaat ggcgatgggg ttggtaacag
     1801 atttggacgc gagcgagagg acgacttcgc gttccgcgcc cagccatttg gcaaaataca
     1861 tccccgtaac aatgcccgta acgctgcccg caagctgtga aacgatgacg ggcagccatt
     1921 ggttgaagat tttacggcgg ttttggtaga gcggcacggc aagcacgacg acggcgggct
     1981 tgagccagaa atcgataaac tgcgcggcgt tgtggtacac cgcataatcg atgtcgagga
     2041 ttttcaggta ggcaatcagc acgatagtgc tgacgagtac ggggttgcag aagatattgc
     2101 ccgtgcgcgt gcgcacgata atcgcaagcg cgtacacggc aagcgtgagg aaaagcagaa
     2161 cgctgggctg cctgaggatt tcgttcatca gataatgctc cgtatccagc ggtgaacctt
     2221 gcctgtaacc agcagtacgc acaaagtgct ggcggaggcg gaaaccagta tcgaaaacca
     2281 atcgtcggca atcaaatcca aatagctgat gaccgccacg cagggcggca cgagaaacag
     2341 cgtcagattc gccatcagcg cgtcggtaag ctgttgcagc caagacgttt tgacccaacc
     2401 cgcctgcaaa agcgcaaaca gcacgcccat gccgacgatg ctgccgggca gtttgatgcc
     2461 tgctaggaaa acggcggttt cgccggcggc gaggcagccg aggatgatga ggagcgcgcg
     2521 gatgatgttc atgttgtgtg tctgtctttt gacggcgttg tgataaatct tgttaactgt
     2581 ttaatcataa cgtcaaatat gccgtctgaa aagccgaagc cgcgcacaaa cgcaaaaacg
     2641 gcacggataa accgtgccgc agcaaccggg gcgtgttcag actttgcggt agggattttc
     2701 caaaccttca atcagcgcga gcaggtaatc gcgcaactga cgttcgccgc aacccatcag
     2761 cagcgcgtct tcaaacgcgt cttgtgccgt ctgatacagc tcggccatat tttcggacat
     2821 cactttgatc ttttcggtac aggacacgat ctgtccgtca tcgtcgtacc atttgggtat
     2881 ttcgggcata ctcattgcgt attccttggg aaatcagttc agaaagcggg acgggtcgtt
     2941 tttcttgacc cggcgggcga acgcgtattt gccgctccaa tatttgtggc tcaggctggt
     3001 gatttcgata tttttccccg tgcgcggcgc gtggatgaag cggttgttgc cgatataaag
     3061 tccgacatgg gaaatgcggc tgccgccgag cgtgcggaaa aacaccatat ctccgggctg
     3121 caattcgctt cgggcaaccg gcgtacccat ccgtgcctgt tctgccgacg tgcgcggcag
     3181 gttgatgccc atggcgcgtt tgaagatgtg ctgcatgaag ccgctgcagt caaaaccggt
     3241 agaaaccgat gtgccgccgt agcggtaggc aatacccaaa agtcccatcg cgttgccgat
     3301 gagttcgtcg gcattgcccg cccgccgggc gggggctcgg ttgatgggta aaacgggctg
     3361 ttcgtcttcg gcaaactgtc tgagaatctg ctcgcggctg ctgagcaggt tggtcaactc
     3421 gtcggcaagg gcggggcgga cggcaaacat cagccacaaa accgcccaaa ctgccggttt
     3481 gaaaaaagaa tccatatcgg tgtttccgcg caggaggcat cgtgccgccg tgcgatgtta
     3541 tgctgtatca gtcgaaaata tcttgttgat tgtatataaa aaaacggctg tttggcaaaa
     3601 aacggcttgt tagattgagt taatatgttg atttttatat aatttattga taatttggga
     3661 agcgcatccg ccgtccgtct tgttttcgcg ccgaccgcaa ccatatagcc gccatccgaa
     3721 caatcagtca gagaaaatca tgaatcaaac cgccatcaac cgcgccgatg tgcgtacccg
     3781 ctttatcttc gacgatatgc ccgtgcgcgg gctgcacgtc cgtctggaaa acgtgtggca
     3841 gcacattgta aaacagaaaa actatcccgc cgccatccgc cgcgccttgg gcgagctgtt
     3901 ggctgccggt gttctgctgt cgggcaacct caaaaacgaa ggcacgctga ttgtgcaggt
     3961 tcaggggcag gggcggctga aaatgctggt tgcggaagcg acttccgacc gtaccgtccg
     4021 tgcgaccgcg cggtgggacg aggcggcaga aatagccgat gacgaaagcc tcggcgacct
     4081 tttgggcggc aacggcgtgt tcgtattgac gctgcagccc aaagacggcg aaccctggca
     4141 gggcgtagtg cctttggaag gcgacggtat cgcgcaaatg ttgatgaact atatgaaacg
     4201 ttccgaacag cttgatacgc acatcgtcct gtctgcaagc gatgaagcgg cgggcggtct
     4261 gctggtgcag cgtctgcctg aagaggtgtt ggatgaagag gcatgggaac acgtcagtac
     4321 gctggcgcgc acgctgacgg cgggcgagtt ggcagaattg gatgcgcaac atgttttgta
     4381 ccgcttgttc cacgaaacgc cgccgcgcgt gttcgagccg gaaacgtttg aattttcatg
     4441 cacctgttcg cgcggcaaag tcagcgatat gctgttgatg ctgggcgggg aagaagtcgg
     4501 cggcgtggtg gcggaacaag gcagcatcga agtcgattgc gatttctgcc acagcaaata
     4561 tgtgtttgac gaaaccgatg tcaacgcgct gttcggggag gatgtggtcg gcgttgccaa
     4621 agggctgccc cggcataccg tccaataatc ttgtgcaaca aggataaata gtcaaatgcc
     4681 gtctgaagca gcttccgctt cagacggcat ttttgcgcgg gttcagacgg catttaaagc
     4741 aggaataggg tggcgagccc caagaaaatc aggaagccgc cggagtcggt aacggcggta
     4801 atcagcaccg agctgcccag tgcgggatcg cgtccgaact tttccattac cacgggaatt
     4861 aatacgccga cggttgccgc cagcaggagg ttgagcgtca tcgcggcaat cataaccagc
     4921 ccgatgccga ggctgccgta gagcagccat gaaaccgcgc ccatgacggt tccccagata
     4981 atgccgttga ctagggcaac gccgacttct tttttcaaca agcgccccgc ctgcattccc
     5041 gtcaactgcc ccatcgccat ggcgcggaca atcatggtaa tcgtctggtt gcccgagttg
     5101 ccgccgatac cggcgacgat gggcatcagc gcggcgagtg caacgatttt ttcgatactg
     5161 ccttcaaacg cgccgatgac gcggctggcg aggaaggcgg tgcagaggtt gacggcgagc
     5221 cacatccagc ggtttttgac ggaatccaag acgggggcga acaggtcttc ctcttcctgc
     5281 aaacctgcca tgttcaacat atccgcttcg gattcttcac ggattacgtc caccatctcg
     5341 tcaacggtaa tcctaccgat gagctttttg ttttcatcga caaccggcgc agttaccaag
     5401 tcgtagcgtt caaacgcctg cgccgcttct tccacgtcgt cttcggcgcg gaaacgcacg
     5461 acatctttcg ccatcacgtt ttccaccaaa tcttcgggat cggcgaccaa aagtttgcgg
     5521 atgggcagca cgccttgcag tacgtcgttt tcatcgacca caaaaatctt gtcggtatgg
     5581 tcgggcaggc tgtcgaagcg gcgcagatag cgcagcacca cttcacaggc gacatcggcg
     5641 cggatgctga ccaactcgaa gtccataatc gcaccgactt ggttgtcttc gtaggacatt
     5701 gccgctttga cttgggcgcg ttcttcctca tcgcgggttt gtagcgcttc gtaaaccact
     5761 tggtgcggca aatcgtctgc cagttccgcc aattcgtccg cgtccaaatc atcgaccgct
     5821 gccaacaatt cgtctttgtc catcgactcg atcagcgttt cgcgcaccgc gtcggatact
     5881 tccagcaata cttcgccgtc gtcttccggt ttgaccagaa tccagacgat attgcgttcg
     5941 cgcggcggta gcgattccaa taccgccgcc acgtcggcag ggtgcagctc gctcaagaga
     6001 acggtcagct cggtcagctt gtcgcgcagc ggcgcgtctt caagcggtgt gccgttttcg
     6061 atttgttcaa aagcaggttc gagaatttcg cagagggagt ggacacggtc gaaatcggcg
     6121 gaaacgcgtt ctacatcgtt ttcgataccg tcgttttcaa ggttcggagg ggttggttcg
     6181 atgctcataa atgctccgcc cgccgtgtgc ggggaggcat tccggcggga tggttattgt
     6241 cgtttggaat cggaaggggc gttcagtaaa aacgaactgg gaaggtccat aataaaagcc
     6301 tgacggtaca ggcgcagggt tggaaacggc actattctac tcccttttgg aacggtttac
     6361 tatttttaac gcaaatgccg tctgaaacgc gggttccaga cggcattttt atcggcttgt
     6421 taccagttgt agcctatttt gattcctgct tgtttgtggc gggcggtgtt gctgtctttg
     6481 ccgtaagcgg catgaagttt ggcgttccag cctttgtatc cggcgtcgat ttgcagggta
     6541 tgcagtgtgc cgtgggcggc ttgctgcagt aaggtacggt cgttgacgct gagggcggaa
     6601 ccgctgtttt ggcgggtatc gtagtaatcg ctgctgaagg cgggggtcag cttggcttga
     6661 cccagttcga cggttttatc gagacggatg ccggcatgcc atgtggtttg gatttgcgcc
     6721 gggctgttta tctctgcgcc gtcgagtacg taccggttgc cgttgctgcg gtttatacgg
     6781 atgccggcat agggtctgag gttgatgccg gtatcgattt tgatgccggt gttgatgcct
     6841 gcatcccatg cgtggcggcg gacggcagcc ccgtcataat cggtaaatcg ggtacggctg
     6901 ttgctgtagc ctaaatctgc cgcggcaaag agtgcgccgt tttccccttt gacgaacaga
     6961 tgtgcgccgt tgctgcggtt tcgggcggat acgccttcat caaaacggtt gtttgtgcgc
     7021 tcatcggtta aaattgtacc gacactgaga cggtcggtga tgccggtttg gatgctgata
     7081 tgtgcatagt tggtagtttg ttggtaggga cggtgtgtgc cgctatggta gtcggtttgt
     7141 tgcgtaccgg tttccagcca gatgttttgc tgctgcggat cggtaaggtg gcggtcgatg
     7201 cggcgtcccg cctgttgccg gccggtattg taggcggcct gttcggaaac ggcggtgttg
     7261 gccgaccggc tgatcagttc ggccaggttc ggcatacgga tgccggcaac gccggttttc
     7321 aaactgtgga tttgtcggga cagacggttg agtgcgttca ggtaggcgtg ccgggctgtt
     7381 tcgacggctt gcgcatcgcc gccttgccgt gctttgtcaa gttcggattc ggccatttct
     7441 acacgttcta tatacgtatc cagttcggtt tcgaagagtg tcaggtcgtt cgtgtcggcg
     7501 gctttggcaa cctgacggca gatatcgaca ctgtatcctt gtgcggcaca caggttttgg
     7561 gcacgcgtca gaatgccggt cagcgggtct gtctgtttca attgggcgga cagatattgg
     7621 acttggctgt cgatgcgggc aagttcggtt tgactgttct gccaggcctg tagtgcctgc
     7681 ctggtcgcgt cagagtcatg ttgaacctgt ctgctgatgt cggttgcctg taattggttg
     7741 tatgcctgtt ggttgcgctc atgttccgca cgcgtggctt caatttgaag ttcggcctct
     7801 ttgagcgggt tgtacaggct gtatccgttg ttgtttttgc ggaggatgta gcggtaggca
     7861 cccaaatcgg catagccgtt ttggagggtg aatcgggctt ggtggctgtg tttcggattg
     7921 aggctcacaa gtgcaagcga ttcggttgtt tgaggttctt gtccggtgtt tttgacgtgg
     7981 atttggaatg cgccgcggct gtccccttcc agtttgaggg ggggggcatt ttgttttcgg
     8041 acgatgccgg tcaggaatcg gaatgtgccg aacccgtcaa gtgtgccgtt gacggtcagt
     8101 gtgttgaagc ggttgttgtg tgtattattg gcgaaatcgg ggttcagggt aatttgtgcg
     8161 ccgtcaagcg tcagtgcgcc ggtgtggctg gactgggaaa gtgtccagtt gctgtctgct
     8221 tccatgcgga ctgcggtgtc tttgccggca cggatgctgc cgtacaggtg tgctttgccc
     8281 aggcggagct ctgaacggtc gttaagggta atgtcgccgc gtacttgcgt tgcaggtagt
     8341 gcacgatagt tttcggcttt taaaacggcg ttgggtgtgc agtgggtgct gccgctatgg
     8401 taggagcggt agcattccgg tgttttgcct tggataaagc cgaggtcgat gccggacaga
     8461 tcgtatgcgg ttatgtcgcc gtccagatgc gcggtattgc gccctgccgt cagtcgggca
     8521 tggtttcgca gggtgaaccg tgcagccttg aagctgccgt cggtccattc gttttcaaga
     8581 acgggttcgc gtttggcctg gtggtcgtag gcatggggta cgggcctgcc tgacacaatc
     8641 atattgccgc cctcaatcaa gacttccccg ttaaggttca tgccgccgtt gagcaacagc
     8701 gtgctgtcgg tgcgtttggg acgatagtgc aggttgagtg cgccgttgta acggtattcg
     8761 ggccggccgt atacggcggc atttgcgata tggcgggttt tttctatggc tgcttcgccg
     8821 gtcatgtccg agaaattccc agtcttgggt gctgtaggca ttccatggga agtaggcgcc
     8881 ggggttgccg ccttctttga ggcggtaatc gtcggtacgc ctgcccctgt gcgggttggc
     8941 gtattcgtat agggcgaggg cttgtgtggg gtgtttggac cattcaccca ttcgatattg
     9001 gttttgctgt aactcggtgc ggctttgccc gtttgcgcgt tctcacccaa gtatccgccg
     9061 aaggtaatta ggtcggggcg ggcattttcg gcttgggcga cttgtttggc ggcctgttgc
     9121 ctgttgttgc cgataaattg ccagcttgtt gagttttcca tatttaacgg gaaaaattcg
     9181 cgcgggttgc cgccgggttt gagtatgaag tagtcggtcc gacggttgcg gtgcgggttg
     9241 atgtattcgt aaaccgccgc gttgccttgc ggacggttgc cccattgcac ccactcgaca
     9301 tgctcgggac tgaggacggg gttgccggtc agcgtcagtg tcgcggcttg gtcagggttg
     9361 tgattgacga tttgcgcgcc gccgtccgca tggcggatat gggtaaaggc aaggttgttg
     9421 ccgttgaggt cgagccgtcc gcccctgaag ccgaaataga ggttttcggg tttgatttgc
     9481 tggctgtcgg cgaggacggc cgtgcccctg ccgctggtga tgccgacttg gttgaatgct
     9541 tgtttgctgc cgtctgaagc agctttttgg gcgagtacga cagtgccttc cccgatgctg
     9601 atgtcgccct ggttgatgcc ttgtccgttg gcgataagcg tgcctgcgcc cagtttggag
     9661 agccggtcgc ctttggggtt gctgacttgc cagaagacgc gtttgccgtc ggctacgata
     9721 acgcctgcac cttgccatgt gtggttttta ccgacgacgg tgaagttgct gtcgaactgc
     9781 aatgcgcctg cgccttggtt gatattgtct gccagcatca gtgttttgtt gtcgaacctg
     9841 ctggatagga tgagcgtttt gccggcatct tcagacggca tgtgcctgct gtcgttttgt
     9901 ggggcaagcg aagggtttgc aatgggcagg gtgatgcgtt cgttgagccc ttgcagggtg
     9961 ctgtcgccat taccgttgtc gcgccatatg agttcgttgg tggtcagccc gacatccagc
    10021 cgggtttcgt attggcggat ggtggaacgg atgaattcgg gttgcgtgac gatgtatttg
    10081 ttgaagaagt tatcgaagcc ggcgtaggtg ctgagtacgc ccgcaagcac ccagcggttt
    10141 tcatgcttgt cgaaggcaaa caggggggaa ccgctgtctc cggcgattgc gtaggtgtta
    10201 aggggttggt cggtcaggct gccgccgacg agtaagccgt ggttttggaa ccccaatact
    10261 ttcagcggcg tgccgccggt caggtattgg tatgccgggg cggttcgtgt acgcgtgccg
    10321 tctgctttgc ggacttgttg cgtgcctgag ccgagtcgta caaagtatgg gaagcggtcg
    10381 gtatcgaggt aggcattggc ctttggctgg ccgtttccaa gcaagggtac gctgctgagt
    10441 gcggcaggtg agatttcggt aaccagtttg ttgaggcggg gaaggtggta gtcgtagtcc
    10501 gggtgcgggt tgcgtgatac gaggcggtag gtataggctt gttcttctgg attttgggtg
    10561 tcgttgccga attgtatgga accgtagccg acgttgtgtt tgacactgtt gacgtattgg
    10621 gggtgaatca gggtggcgat ggcggtttgc cggttgctga ctcggaagtc gggcatgggg
    10681 atgccgttga gaaccctgcc taatattttg ccttgcttgt cttggatgga aatattgctt
    10741 gcacctacgg cgaacgcgcc tttattttcg gcaaagtcgc gaaaatattg gtaatcgaca
    10801 tcgttgcgga caatggatga gtaagcaggg gaaacggcaa aaagaccgag ggtggacaat
    10861 acggaacaaa atggggtggt gtgtgtgaag cgcatgatga ttatccgtgt aatagagaaa
    10921 atcaacgatt cttgcggaca agaatcaccg atcccgtttt gtgctggatt tatatagtgg
    10981 attaacaata tagtggatta acaaaaacca gtacagcgtt gcctcgcctt gccgtactat
    11041 ctgcggcttc gtcgccttgt cctgatttaa attcaatcca ctatattata tattttgttt
    11101 ttgtttgatt ataaggcaac ggtgtccttt cgtctattgg cgatgattgt atgattttat
    11161 ttaagatttt aataaaaatc ctaggaatgg ctgcgttttc ccattgtttt atttcgtccg
    11221 gtattggttt ttaagttcac gcagcgcgac aaaggtatcg aggctgctgt ttaacgtttt
    11281 cccgctcaat gcctcggcgc ggtctctgac gtgcggcagg tcgacgcgca aaaacggatt
    11341 gacgcggcgt tcgtgcgcga gggtaacggg cagggtaggc gtatgcgccg ccgctttcag
    11401 tgccgtctga atgtcggcgt tgtccggctc gatatgggcg gcgaaacgca ggttggcggc
    11461 ggtgtattcg tgtgccgggt agaacagggt gttttcaggc aggcggttga agcgttggaa
    11521 gctgtcgtaa agctgttcga ttgtgccggt aaacacgcgt ccgcagccgg cggaaaaaag
    11581 ggtgtcgccg caaaagacgt gtatgccgtc tgaagtttcg agaaggtagc tggtgtggcg
    11641 gtctgtgtgg ccgggtgttg cccaaacggt aacctgtccg tcgccgaagg tgaattgggt
    11701 gccggcggtt acggtgtggg ttgctgcttc gatgtcggat tcgccgtaaa cgggcgattc
    11761 catgtagccg cgccagagtg ccgccgcacc gccctcgtgg tcggggtggg ggtgagttac
    11821 ccatgtttgg gcaagcatga ggcggttgcg gacgaggaat tctaagacgg gcgagggttc
    11881 ggaagggtcg acgcagacgg catggttgcc gtgctgtatc atccagatgt agttgtcggt
    11941 tagggctttg acgggggtga ttttcatgag tgttcctcgg tggacggata ccgtttaaag
    12001 gatggtatgc cggaacgccg tctgaaatat tcttcagacg gcattggttt gagtagggcg
    12061 gttagcccaa ggctttacgt gcgccggcaa agaggcggta ccagccggac agttccgtcc
    12121 agccttccgg tttccagctc atttgcgcgg cgcggtacac gcgttcgggg tggggcatca
    12181 tgatggtcac gcggccgtcg gcgttggtaa tgccggcgat gccttgcggc gagccgttgg
    12241 ggttgagcgg ataggtttgg gtaacctggt tttgaccgtc cacatattgc agcgcgatgc
    12301 ccaaatcggc ggaaatattg ccgccgtgaa gcgcgaagtc ggcgcggcct tcgccgtggc
    12361 tgaccacgac aggcaggctg gagccttgca tttcgttcag aatcagcgac gctgatttcg
    12421 gaacgtgaac catgctcagg cgtgcttcaa actgttcgct caggttgcgt ttgaatttag
    12481 gccatgcctc tgcacccggg ataatctcgg cgaggttgct gaccatctgg cagccgttgc
    12541 acacgcccaa tgtcagcgtg tccggatcgg caaagaaggc ggcaaattgg tcgcgcaagg
    12601 cagggtggaa caggatggat ttcgcccagc cttcgcccgc gccgagtacg tcgccgtagc
    12661 tgaagccgcc gcacgccgcc agcattttga agtcggcaag gcggaagcgg cctgccatca
    12721 ggtcggacat atgcacgtcg taggcatcga aaccggcgcg agtgaacgcg gcggccattt
    12781 cgatttgccc gtttacgccc tgttcgcgca ggatggcgat tttgggtttc gcgccgctgt
    12841 tgataaacgg cgcggcgatg tcttcgttca cgtcgaactt cacgtcggca aacaatgcgc
    12901 tgcgttcgtt gtcgccaatc agggcaaact cgctgtcggc gcaggcaggg ttgtcgcgca
    12961 ggcgttggat ttgatggctg gtttcttgcc aggtttgttg cagtttgatt aggttgtcag
    13021 aaataaggtg cgtttgcccg tcgcggatga ttaacgtgtt ctcatcagtt aacgtaccga
    13081 tttcaaagac attatgatgc agctgttgtt gatagaataa attgataata tcggcaacat
    13141 cttgttttct aacttggata acagcaccca actcttcatt aaataatgtg cgggcaatgg
    13201 tttcttgcca ttcagccaac gcttttacct cttcagtccg caatgattga gacagagcgg
    13261 tatggttggt aataaatgtt tgtgcaagca ataaatttaa atctatatcc aagccgcacc
    13321 gccccgcaaa cgccatttct accaaaacgg caaacaaacc gccgtcgctg cggtcgtgat
    13381 acgccaagag tttgtcttcg gcgacaagct gctgaatcac gttgtaaaac gctttcagac
    13441 gacttgtatc gtccaaatcg ggcgcgtcgc cgctcatatt gttgtacacc tgaccaaacg
    13501 ccgagccgcc catacgcgct ttgccgaagc ccaaatcgat aaacagcaat acgctgtctt
    13561 cgacgttttt cagttcgggc gtaacggttt tgcgtacgtc ttccaccggc gcgaacgcgg
    13621 agataatcag gctcaatggc gagacgacgg atttcttgtc cgcgccatcc tgccaaacgg
    13681 ttttcatcga caggctgtct ttgcccacgg ggatgctcaa atccaatgcc tgacaggctt
    13741 tggaaaccgc ttcgacggtg cggtagagtt tttcgtcttc gccttcgttg ccgcacgccg
    13801 ccatccagtt ggcagaaagt ttgatattgc cgatgtcgcc gatgttgacc gctgcgatgt
    13861 tggtgatggc ttcgccgata cacattctgc ccgaagcagg cgcgtcaaac agggcgacgg
    13921 ccggtttttc gcccatagac atcgcttcgc cgcgataggt gttgaagccc atcatggtca
    13981 cggcgcagtc ggctacgggg gtttggtatt tgccgaccat ttggtcgcgg tgggtcatgc
    14041 cgccgacgct gcggtcgccg atggtaatca ggaagttttt agccgctacg gcaggcaggc
    14101 gcagaacgcg gtaggcggct tcggtaatgt cgatatcgcc ggcattaaac ggttttttcc
    14161 caaagggatt cccttcggtc gcatacggcg taaccgtttt gtcggtgcgc gtggttttgg
    14221 gcggtttgcc gagcaaaacg ttcaacggca aatcgacagg gttgttggag aacaaatcgt
    14281 cgcgtacttt caaatggccg tcgccggtcg ccgtgccgac tacggcaaac gggcagcgtt
    14341 cgcgttcgca gatggcgcgg aaggcatcca aatctttttc caaaatcgac aacacataac
    14401 gctcttgcga ttcgttgcac cagatttgca aaggcgttaa accgtgttct tccaacggca
    14461 cttcgcgcaa cttgaatacc gcgccgcgtc cggcatcgtt gacgagttcg gggaaggcgt
    14521 tggacaaacc tcctgcgccc acgtcgtgga tggagataat cgggtttttg tcgccgagct
    14581 gccagcagcg gtcgatgact tcctgcgcgc ggcgttcgat ttcggggttg ccgcgttgca
    14641 cggagttgaa gtctaaagaa gcgtcgtttg taccggtatc catcgaagaa gccgcgccgc
    14701 cgcccaagcc gataagcata cccgggccgc ccagttggat cagcaacgcg ccttcgggga
    14761 tttcgtcttt atgcgtctgc tgcgcctgaa tgctgcccaa gccgcccgca atcataatcg
    14821 gtttgtggta gccgcgaacc tgaccgtcaa atttttcttc aaaggtgcgg aagtagccca
    14881 agaggttggg gcgtccgaat tcgttgttga acgccgcgcc accgatgggg ccttcgatca
    14941 tgatgtctaa aggggaagca atatggtcgg gtttgccgta ggcttgttcc caaggctgtt
    15001 tgaggtcggg gatgttgagg ttggagacgg taaagccggt caagcctgct ttcgggcgcg
    15061 aacctttgcc cgtcgcacct tcgtcgcgga tttcgccgcc cgcgcccgtc gccgcgcccg
    15121 caaacggcgc gatggcggtc gggtggttgt gcgtttccac tttcatgatg atatgcgtgt
    15181 cttcctcgtg gaaacggtag ccttggtttt ccgccgcatc cggatagaaa cgctcgattt
    15241 tcgcgccttc aatcacggac gaattgtctt tataagcgac gaccgtgcct tcgggatgcg
    15301 cgttgtgcgt gtcgcgtatc ataccgaaga gggatttcgg ctgtttttcg ccgttgagga
    15361 tgaagtcggc gttgaagatt ttgtggcggc agtgttcgct gtttgcctgc gcgaacatca
    15421 tcagttccac atcggacgga ttacggttta aagcctgata gttttcaacc agataatcga
    15481 tttcgtcggc ggagagtgcc aaacccattt cggtattggc tttgactaaa gcctctttac
    15541 cgccgcccaa aacatcgacg gtagagaagg tttcggattc gagatggtgg aataatttgg
    15601 atgccgtctg aaaatcgggc aatacggatt cggtcatgcg gtcgtgcagc aacgccgccc
    15661 attgctgttt ctgttcatcg gtaagcgcac cttccagcca caccgccatg ccgcgctcga
    15721 tgcgttcgat gcctgccaaa ccgcagtttt ccgcgatatt ggtcgccttg gaagcccacg
    15781 gcgaaatcgt acccaaacgg ggcgtgacca aaaacaaatg caagccctcg cgcgcttttg
    15841 gcgtttgttc aacgctttgc gccgccaaca aggcttgcag tttttcgaca gtcgcggcat
    15901 ccaatgcttt ctcgctgccg acgaaatacc aaaattcgct gcttaatttg acttcgggca
    15961 gaccgagtgc ggctgctttt tgcaagagtt tttcaacacg gaaatcggaa agggcggtaa
    16021 cgccgcgcaa gggcaaaacg acagacatgg attcggctct caaatgcggt tgggaaaaag
    16081 agggattata cgccggacag gtttgtattg cttggatatt ttggcttaaa atgttgacaa
    16141 tcttcagacg gcatacagtt ggggaaaata caggtgccgt ctgaagatat taaggaataa
    16201 gggttttatc tgtttttctg atttttaccc aaagccgggt atgcggataa ttgcaggaag
    16261 tagaggggcg ggcaactgct tccgtatgtt tttagatcaa atgccgtcgt ttccgcacgg
    16321 ttaggaatat ggaagtttgg atttacagaa acctttaaat attgctgaaa tgcagagatc
    16381 gggattcccg cctgcgcggg aatgacggtt tccggcaaat cgccgcatcg ccgggcgaac
    16441 agtaccgttt atcgtttgcc ggcggtttct gctatatttg tcgtgtgttt acgttaatta
    16501 atggagaatg tgatgaaaaa aatcgaggcg attgtcaaac cgttcaaact cgacgacgtg
    16561 cgcgaggcgt tgacggaaat cggcattacg ggcatgaccg tcagcgaggt caaagggttc
    16621 ggcaggcaga aggggcatac ggaaatctat cgcggtgcgg aatacgccgt cgatttcctg
    16681 cccaaagtca aaatcgagct ggtgttggcg gatgatgctg tggaacgcgc gattgacgtg
    16741 attgtcgagg tggcgcgttc gggaaaaatc ggcgacggca agatttttgt gctgccggtc
    16801 gaggaggcaa tccgtatccg cacgggcgaa cgtgcggacg cggcggtttg agcgtttgga
    16861 atggaaatgc cgtctgaaag gttttcagac ggcattttct ttttgagctt cagatatggt
    16921 ggacgatgag ttttttgctt tggtggtcga ggacggacac atccatatcg aacagggttt
    16981 tgatgtttgc ggcggtgaaa atgtcgtcgg gcttgccctg catggcgact tggccgtttt
    17041 tcatggcgac gacgtggtcg gcgtaggcgg cggcctggtt gatgtcgtgt aatacgacga
    17101 cggtggtgcg cttgtgttcg tcggtcagcc ggcgcaggat ttgcatgagg gagcgggcat
    17161 gatacatgtc gaggttgttc agcggttcgt ccagcaaaac gtagtcggtg cgctggcaga
    17221 acaccatggc aatcatggcg cgttggcgtt gtccgccgga aagctcggtc aggtagcggt
    17281 cggcgaagtc ttgcaggtgg aattcttcga ttgcaccgtt aacgatacgg cggcattcgg
    17341 cagtcggtct gccttgatgg tagggatagc ggccgaacat cagcaggtcg cgcacggtaa
    17401 tgcggctcat gatgctgttt tcttgggtga ggatggacag agttttggcg agttcggcgg
    17461 tgggggtgtc ggcaaggttt ttgccgcggt aggcgatgct gccgctttca agcgggcgca
    17521 gccgcgccat aaaggaaaac agggtggatt tgcccgcacc gttggggccg acgagggcgg
    17581 taatgccgcc ttcgggaatg tcgaggctga cgttgtcgag gatggggcgt gtgccgatgc
    17641 ggtagctgac gttgcggatg gtaatcatgg cggatgcttg tcgggcgtag gcttgttgtt
    17701 gtttggcggg ggttcagacg gcatttgaaa ttcggaatgc cgtctgaacg ctcacacttc
    17761 gcggacgatg acgaactgcg ggtcgtccgc cgattcggtc aaaaatgctt taacgcgcgc
    17821 ctgccagagt tgtccgaagc gttcgagttc ttctgcactt gcgctgccgc tgacggcttt
    17881 gggcatgatg tcgcgcattt gcggcgcaaa gggttggagc gcagcgttga ggctgacggc
    17941 aacggttttg ccggtatcgc ggcggcggag cgcgagtgtg ccgttgattt cgcctgcacc
    18001 aaaggataag aggttgcggc gggcaaagcg tccctgcatt ccgatgccgc caaagccggt
    18061 ttcgggggct gcgccggtga ggagttggac gacggacgcg gttacgccga ccgtgccttc
    18121 gtcgcgcgcg ccctgcatgg cggcttcgat gccgccgcgt tcgggcagct cttcgccgta
    18181 aagtgctttc agtcctttga taaccatcag gtacgcgccc gcgacggtcg ggcaggaatg
    18241 tccgcacagg cgcacggcat cggcgtagcg gtaagtgagg atgccgtttt cgaccgcgcc
    18301 gaggaatgcg gcaagcgggt ctctgacggt gatggtcggg gcttggttga agaatgatgg
    18361 gaaacgttct tgcgtcatat ggtttgtcct ttcgagtcgg gctgccgttc agacggcatc
    18421 cgtcattttt tgtgttttaa aacgagatag aggaaaacga gtccgcccgc aaattcgacc
    18481 accacgctta ataccgcctt catgcccaag aagtgttcga atacggtctg tccgccgacc
    18541 aagaggatgc cgccgacaca aaccgtcatc ggcaggcgga cggaatggcg cacggacggg
    18601 gaaaagtggt tggcaagcga ggcggcgaga agcccgaaaa agcttaccgg gccgacaacg
    18661 gcggtcgccg tcgccaccaa tacggcaatc caaagcagta tccataaggt gttgcgcgtg
    18721 tagctgatgc ccaaattgac ggcttggtca cgccccaaaa ggtgtacgtc caagcggtag
    18781 cgttcacgcc aaacgaccgc cgcgctgacg agcagaatca gcgcgcctat gcccaaaagc
    18841 tcgctgtgga cggtattgaa tccggcaaac atattcgcct gcgccgccgt aaattcttcg
    18901 ggatcgatca tgcgcgaaag cagcgatgac aggctgcgga acaaaatccc gaaaatcacg
    18961 ccgattaaaa tcatacgcgg caaatcgcgc ccgccctgac ggatgagcgt gtagaacaac
    19021 agcagcgagc cgcccatcat aacgaccagt tcaaagccga atttgcccgt caacggcagg
    19081 gaagcatagc ccacgccgcc cagagcgaaa accaaaagcg tctgcaaaaa cacatacagt
    19141 gaatcgaaac ccaaaatcga aggggtcaga atcggattgt tggtcagcgt ttggaagagt
    19201 tgagtggaca cgccgaccgc ataggcgacc atcagcagag cggcaagttt ggtcagacgc
    19261 aggtgcagta caaagtccca atcgcctttg acgttgagcg tcataaacag gatgcaggaa
    19321 accagcaaca gcgcaaaggc aacccacaac ggacggctgc ttcctgccat aaaaccgata
    19381 tttttttcag acggcatagg cgggtttcct taacaaaagc cataaaaaca acgccgtacc
    19441 caataccccg aagacggtcg ataccggaat ttcaaacgga aacacaatca cgcgtcccaa
    19501 aatatcgcac agcaacacca aagacgcgcc caacaaagcc accgcaggca ggctttggcg
    19561 caacctgtcg cctatcaggc ggctgatgat gttcggcacg accagcccga taaacggaat
    19621 attgccgacc gtaacgataa ccagcgacgt aatcaaagcc acaataatca aacccgacca
    19681 caacaccgcc gtccggttca aacccaaatt cacgcttacc gtttcgccca gccccaaaat
    19741 cgtcagccgg tcggcaatca gataggcaaa caccgccaaa ccgcccgtaa tccaaagcag
    19801 ctcgtaccgc cccagcagca cgctcgaaaa atcgccctgc tgccacacgc cgagcatttg
    19861 cagcatttcg ttttcatacg cgataaaggt ggctaccgcc tcaatcacac cgccgaaaat
    19921 aatcccgacc aaaggcacca tcagttgcgc ggtcggcggc aggcggcgga tcagcagcat
    19981 aaagaccaac atcccgatca gcgcggcaac ggcggcaacc gacattttcg ccggcagcgg
    20041 cgcggccggc agcagcaggg tcatcagcag caaacctaaa gccgcgcttt ggcttgcgcc
    20101 caccatcgac ggttcgacaa aacggttgcg catcaaaatc tgcataatca tgccggccac
    20161 cgccatcgac gcgcccgtca gcacaatcgc aaacgtgcgc ggcaggcggc tgatgaacat
    20221 gacctgctgg ctgtcggaca gtgaaaacac atcagaccag cggaaatcgg caacgcccac
    20281 cgacaggctg acggcaaaca acaccgccag cagcagcagg ttggtcaggt tgagggaaaa
    20341 aggtttggca gtcataaaca gaagggaaaa gcgttaaggc gtagaagatt caaacaaggc
    20401 agtccgaacc gtcggagcgg aaagccttgt ttgaagcctc cgtatcgggc aatgccgtct
    20461 gaaacacagg aggcggtttg catccgtgtt tcagacggca tcgaatgccg ccgtttcatt
    20521 attttgccgc gttaaaagcg tcggcaacct gtttgcttgc attcagcagc tcttgcgcgc
    20581 caccggctgc caaataagtt tcaggaacga ggtacacgac ctgtcctttt ttccaagcgg
    20641 ttgtttcggc aaccagcgga ttatccaaca cgtctttcgc cgcctgaccc tcttcgccga
    20701 tggccgcgct tcggtcaagg acaaacagcc agtcgggatt tttctctttc aggtattcaa
    20761 agctgatagg ctgaccgtgg ctgccttctt taattgattc atcgacagcg ggaacgccga
    20821 tgtctttgtg cagccagccg cccaagcgtg aagacgggcc gaaagccgac atcttgccgc
    20881 cgttgaccaa aatcaccaaa cctttgccct taccttgtgc ggcagttttc gcggcttcaa
    20941 aagacgcgtc gatttccgcc ttcagcttgt cggcttccgc ctgtttgccg aagatttgcg
    21001 ccagcgcgtc gatgcgctct ttggcacttt ctttgaggtt ggcggtatcg gcggtcattt
    21061 cgatggtcgg cgcgatttcg ttcaatttgt caaatgcttt ggcggcgcgg ctgccgatga
    21121 tgatgagctg cggtttgtaa gcgttgagcg tttcgtaatc cggctcgaac aaagtgccgg
    21181 caggttttgt cgttttgaaa tattcctcta aatacggcag gcggttttta tcgacggaca
    21241 aaccggtttt cacgcccagt ttgctcaagg tgtcgagcat acccaaatcg taaacggcga
    21301 tgcgttcggg gttttgcggt atttgaacgt cgccgcgcgc ggttttgacg gtaacggacg
    21361 cgccttcggt ttgtgcggcg gaaaccgcct gttctttggc ttgtggggca gagtcggaat
    21421 tttgcggcga acacgcgccc aaagcgaggg cggtgcatac ggctaaagca gtcaaacgta
    21481 acataggtgt ctccaaaatg gggatattgg ggcaaagccg ccggtcggac aaaccggaac
    21541 ggctttagaa aggataaatg ataa
//
LOCUS       DAWHSK010000010        19233 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.010, whole genome shotgun sequence.
ACCESSION   DAWHSK010000010 DAWHSK010000000
VERSION     DAWHSK010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19233
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.010"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..522
                     /gene="rsgA"
                     /locus_tag="WIC03_000271"
     CDS             <1..522
                     /gene="rsgA"
                     /locus_tag="WIC03_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEZ3882833.1"
                     /translation="NKADLPETALWREKLKFYETLGYPVIETRALENAGSLRPALQGH
                     SNILLGQSGMGKSTLTNALLGSQTARTGDISAALDSGKHTTTHARLYDLNGETQLIDS
                     PGLQEFGLHHLQAADLPRYFPDFRHLVGQCRFHNCTHRAEPGCAFKAAAQTGAASPER
                     LAFLQGITDELPG"
     gene            783..1007
                     /locus_tag="WIC03_000272"
     CDS             783..1007
                     /locus_tag="WIC03_000272"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEZ3882834.1"
                     /translation="MKKNAPKSFEEALSRLESLTQAMQGEMPLEDALAAYQEGNELVR
                     YCQTKLAQVEQKLQVLDADGPKELNLESDE"
     gene            991..1887
                     /locus_tag="WIC03_000273"
     CDS             991..1887
                     /locus_tag="WIC03_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEZ3882835.1"
                     /translation="MNPTNDLKAWQQRAQAQTELLLERFLPSENEIPHTLHEAMRYAV
                     LDGGKRLRPMLVLAASELGEAVHEAVEQAMAAIEMIHAYSLVHDDMPAMDNDSLRRGK
                     PTCHIKYGEATALLVGDTLQTQAFDVLSRPTELPAARQLAMLSVLAKAGGSAGMAGGQ
                     AIDLANVGKQMVQADLEQMHSLKTGALIRAAVLLGAMSCPNLSEDSLAVLDDYAAKLG
                     LAFQVIDDVLDCEADTATLGKTAGKDADNDKPTYVKLMGLEAARSYAHKLVAEAVALL
                     EPFGDKALRLRQLAEFAVARKY"
     gene            complement(1996..2295)
                     /locus_tag="WIC03_000274"
     CDS             complement(1996..2295)
                     /locus_tag="WIC03_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2818 family protein"
                     /protein_id="HEZ3882836.1"
                     /translation="MTASMYILLVLALIFANAPFLTTRLFGVAALKRKHFGHHMIELA
                     AGFALTAILAYILESRAGSVHDQGWEFYATVVCLYLIFAFPCFVWRYFWHTRNRE"
     gene            complement(2407..3852)
                     /gene="nuoN"
                     /locus_tag="WIC03_000275"
     CDS             complement(2407..3852)
                     /gene="nuoN"
                     /locus_tag="WIC03_000275"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HEZ3882837.1"
                     /translation="MNWSDLNLMPAMPEIVLLALLVLLLLADLWVSDDKRRWTHYGAL
                     ATVAVTAVVQLAVWEQGSTSSFNGMYIADGMSRLAKMVLYALMFTLFVYAKPYNQVRG
                     IFKGEFYTLSLFALLGMGVMVSAGHFLTAYIGLELLSLALYALIALRRDSGFAAEAAL
                     KYFVLGALASGLLLYGISMVYGATGSLEFAGVLASSFNEEANEWLLKLGLVFIVVAVA
                     FKLGAVPFHMWVPDVYHGAPTSVTALVGTAPKIAAVVFAFRILVTGLGTVHHDWSLMF
                     ALLAAASLLVGNLAAIMQTNIKRMLAYSTVSHMGFILLAFMAGAVGFAAGLYYAITYA
                     LMAAAGFGVLMVLSDGDNECENISDLAGLNQHRVWLAFLMLLVMFSMAGIPPLMGFYA
                     KFGVIMALLKQGYVWLSVFAVVMSLIGAFYYLRVVKVMYFDESGRARPAAGGNNAAKF
                     LLSINALLLVLWGIMPQTVIDWCAKALENTL"
     gene            complement(3862..5358)
                     /locus_tag="WIC03_000276"
     CDS             complement(3862..5358)
                     /locus_tag="WIC03_000276"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEZ3882838.1"
                     /translation="MFSNYLLSLAIWIPIAAGVLVLSTGKDSRAPLARVLAFMGALAG
                     FLVTLPLFTGFDRLSGGYQFTEFHEWIPLLKINYALGVDGISVLFIILNAFITLLVVL
                     AGWEVIQKRPAQYMAAFLMMSGLINGAFAAQDAILFYVFFEGMLIPLYLIIGVWGGPR
                     RVYASVKLFLYTLMGSLLMLVAMVYLYYQTGSFSIVDFQNIKQIPLGVQQLLFAAFFL
                     SFAVKVPMFPVHTWLPDAHVEAPTGGSMVLAAITLKLGAYGFLRFILPIMPDAARYFA
                     PVIIVLSLIAVIYIGMVALVQTDMKKLVAYSSISHMGFVTLGMFLFVNGQLNDWALKG
                     AIIQMISHGFVSAAMFMCIGVMYDRLHTRNIADYGGVVNVMPKFAAFMMLFGMANAGL
                     PATSGFVGEFMVIMGAVKVNFWVGALAAMTLIYGASYTLWMYKRVIFGAIHNPHVAEM
                     KDINCREFTILAVLAVAVLGMGLYPNAFIEVVHQAANDLIAHVAQSKI"
     gene            complement(5447..7471)
                     /gene="nuoL"
                     /locus_tag="WIC03_000277"
     CDS             complement(5447..7471)
                     /gene="nuoL"
                     /locus_tag="WIC03_000277"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEZ3882839.1"
                     /translation="MNDMTLYLIIALVPLAGSLIAGLFGNKIGRAGAHTVTILGVAVS
                     AVLSAYVLWGFLNGSRAKFDENVYTWLTMGGLDFSVGFLVDTMTAMMMVVVTGVSLMV
                     HIYTIGYMHDEKVGYQRFFSYISLFTFSMLMLIMSNNFIQLFFGWEAVGLVSYLLIGF
                     YFKRPSATFANLKAFLINRVGDFGFLLGIGLVLAYFGGSLRYQDVFAYLPNVQNATIQ
                     LFPGVEWSLITVTCLLLFVGAMGKSAQFPLHVWLPDSMEGPTPISALIHAATMVTAGL
                     FMVSRMSPIYEMSSTALSVIMVIGAITALFMGFLGVIQNDIKRVVAYSTLSQLGYMTV
                     ALGASAYSVAMFHVMTHAFFKALLFLAAGSAIIGMHHDQDMRHMGNLKKYMPITWLTM
                     LIGNLSLIGTPFFSGFYSKDSIIEAAKYSTLPGSGFAYFAVLASVFVTAFYAFRQYFM
                     VFHGEEKWRSLPEHHDDHHGEEHHSLGKNDNPHESPLVVTLPLILLAIPSVIIGYVAI
                     EPMLYGDFFKDVIFVNADAHPTMHIMKEEFHGALAMVSHSLHSPVLYLAIAGVLSAWL
                     LYVKLPHLPAKIAQTFRPIYVLFENKYYLDALYFNVFAKGTRALGTFFWKVGDTAIID
                     NGIVNGSAKLVGAIAAQVRKAQTGFIYTYAAAMVFGVLVLLGMTFWGLFR"
     gene            complement(7520..7861)
                     /locus_tag="WIC03_000278"
     CDS             complement(7520..7861)
                     /locus_tag="WIC03_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3882840.1"
                     /translation="MMDKNQLEQEFHKAMLNIYQEALNLPQPYKATRFLQIVNEFGGK
                     EAADKLLSTGEKKTQTGFTELILSGGGVHALKYSMEYLVLQKPWCDLFTEEQLAVARK
                     RLERVGFVFPK"
     gene            complement(7985..8560)
                     /locus_tag="WIC03_000279"
     CDS             complement(7985..8560)
                     /locus_tag="WIC03_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family protein"
                     /protein_id="HEZ3882841.1"
                     /translation="MPSENPIGKTMKSIDEQSLHNARRLFESGDIDRIEVGTTAGLQQ
                     IHRYLFGGLYDFAGQIREDNISKGGFRFANAMYLKEALVKIEQMPERTFEEIIAKYVE
                     MNIAHPFLEGNGRSTRIWLDLVLKKNLKKIVNWQNVSKTLYLQAMERSPVNDLELRFL
                     LKDNLTDDVDNREIIFKGIEQSYYYEGYEKG"
     gene            complement(8594..8899)
                     /gene="nuoK"
                     /locus_tag="WIC03_000280"
     CDS             complement(8594..8899)
                     /gene="nuoK"
                     /locus_tag="WIC03_000280"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEZ3882842.1"
                     /translation="MITLTHYLVLGALLFGISAMGIFMNCKNVLVLLMSIELMLLAVN
                     FNFIAFSQHLGDTAGQIFVFFVLTVAAAESAIGLAIMVLVYRNRQTINVADLDELKG"
     gene            complement(8896..9567)
                     /locus_tag="WIC03_000281"
     CDS             complement(8896..9567)
                     /locus_tag="WIC03_000281"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEZ3882843.1"
                     /translation="MTFSAILFYILAAIVLYGAVRTVTAKNPVHAALHLVLTFCVSAM
                     LWMLMQAEFLGVTLVVVYVGAVMVLFLFVVMMLNIDIEEMRAGFWRHAPVAGVVGTLL
                     AVALILILVNPKTDLAAFGLMKDIPADYNNIRDLGSRIYTDYLLPFELAAVLLLLGMV
                     AAIALVYRKTTNPKRMDPADQVKVRADQGRMRLVKMEAVKPQVESAEESEVSDGLKPE
                     GEGKA"
     gene            complement(9579..10058)
                     /gene="nuoI"
                     /locus_tag="WIC03_000282"
     CDS             complement(9579..10058)
                     /gene="nuoI"
                     /locus_tag="WIC03_000282"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEZ3882844.1"
                     /translation="MANLVKTFLLGELVKGMGVTLKNFFARKDTIYFPEEKTPQSVRF
                     RGLHAQRRYPNGEERCIACKLCEAVCPAMAINIESEEREDGTRRTKRYDIDLTKCIFC
                     GFCEEACPTDAIVETHIFEYHGEKKGDLHMTKPILLAIGDKYEAEIAKRKAADAPYR"
     gene            complement(10139..11215)
                     /gene="nuoH"
                     /locus_tag="WIC03_000283"
     CDS             complement(10139..11215)
                     /gene="nuoH"
                     /locus_tag="WIC03_000283"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEZ3882845.1"
                     /translation="MQEWFQNLFAATLGLGDLGITVGLVVSVIVKIVIILIPLILTVA
                     YLTYFERKVIGFMQLRVGPNVTGPWGLIQPFADVFKLLFKEVTRPKLSNKALFYIGPI
                     MSLAPSFAAWAVIPFNEEWVLTNINIGLLYILMITSLSVYGVIIAGWASNSKYSFLGA
                     MRASAQSISYEIAMSAALVCVVMVSGSMNFSDIVAAQAKGIAGGSVFSWNWLPLFPIF
                     IVYLISAVAETNRAPFDVAEGESEIVAGHHVEYSGFAFALFFLAEYIFMILIAALTSL
                     MFLGGWLSPFPQSWGFIGTPSAFWMFVKMAAVLYWYLWIRATFPRYRYDQIMRLGWKV
                     LIPIGFAYIVILGVWMISPLNLWK"
     gene            complement(11218..13479)
                     /locus_tag="WIC03_000284"
     CDS             complement(11218..13479)
                     /locus_tag="WIC03_000284"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit G"
                     /protein_id="HEZ3882846.1"
                     /translation="MLQIEIDGKQVSVEQGATVIEAAHKLGTYIPHFCYHKKLSIAAN
                     CRMCLVNVEKAPKPLPACATPVTDGMIVRTHSAKAREAQEGVMEFLLINHPLDCPTCD
                     QGGECQLQDLAVGYGKTTSRYTEEKRSVVGKDMGSLVSAEEMSRCIHCTRCVRFTEEI
                     AGLQEIAMVNRGEHSEIMPFIGKTVETELSGNVIDLCPVGALTSKPFRFNARTWELNR
                     RKSVSAHDALGSNLIVQTKDHTVRRVLPLENEAINECWLSDRDRFAYEGLYHESRLKN
                     PKIKQGGEWMDVDWKTALEYVRSAIECIAKDGNQNQVGVWANPMNTVEELYLAKKLAD
                     GLGVKNFATRLRQQDKRLSDDLKGAQWLGQSIESLADNDAVLVVGANLRKEQPLLTAR
                     LRRAAKDRMALSVLAGSKEELLMPLLSQEAAHPDEWAGRLKNLSANAEHAVTASLKNA
                     EKAAVILGAEVQNHPDYAAIYAAAQELAAATGAVLGILPQAANSVGADVLGVNSGESV
                     AEMANAPKQAVLLLNVEPEIDTADGAKAVAALKQAKSVMAFTPFVSETLLDVCDVLLP
                     IAPFTETSGSFINMEGRLQSFHGVVQGFGDSRPMWKVLRVLGNLFDLQGFEYHDTAAI
                     LKDALDVESLPSKLDNRNTWTGEGVQTTSDRLVRVGGVGIYHTDSIVRRSAPLQETGH
                     AAVPAARVNPNTLARLGLQNGQTAVAKQNGASVSVAVKADAGLPENVVHLPLHTENAA
                     LGALMDTIELAGA"
     gene            complement(13769..14206)
                     /locus_tag="WIC03_000285"
     CDS             complement(13769..14206)
                     /locus_tag="WIC03_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3882847.1"
                     /translation="MVEAKIFILYGAANKGKSTTLNTLFNQICRKFSKFLVFFERHGN
                     GLDFVAVFDHEGQRIGFYSSGDNEYEVRRNLYKLYSHNCDFIFGTSRTRGGSCDAVGC
                     YAELLHGDVNIINWCEKFEPTDEDNERAVKELFKSFKNIINKL"
     gene            complement(14206..15507)
                     /gene="nuoF"
                     /locus_tag="WIC03_000286"
     CDS             complement(14206..15507)
                     /gene="nuoF"
                     /locus_tag="WIC03_000286"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEZ3882848.1"
                     /translation="MAIYQSGVIFDQVDTANPDCWTLDEYVKRGGYTALRKILSGNIS
                     QTDVIDEVKTSGLRGRGGAGFPTGLKWSFMPRSFPGEKYVVCNTDEGEPGTFKDRDII
                     MFNPHALIEGMIIAGYAMGAKAGYNYIHGEIFEGYQRFEAALEQARAAGFLGKNILGS
                     DFEFELFAHHGYGAYICGEETALLESLEGKKGQPRFKPPFPASFGLYGKPTTINNTET
                     FSSVPFIIRDGGQAFADKGIPNAGGTKLFCISGHVERPGNYEVPLGTPFAEVLKMAGG
                     MRGGKKLKAVIPGGSSAPVLPADIMMQTNMDYDSISKAGSMLGSGAIIVMDEDVCMVK
                     ALERLSYFYYDESCGQCTPCREGTGWLYRIVHRIVEGKGKMEDLDLLDSVGNQMAGRT
                     ICALSDAAVFPVRSFTKHFRDEFVHYIEHGGPMKEHKWGGW"
     gene            complement(15897..16370)
                     /gene="nuoE"
                     /locus_tag="WIC03_000287"
     CDS             complement(15897..16370)
                     /gene="nuoE"
                     /locus_tag="WIC03_000287"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEZ3882849.1"
                     /translation="MLSAESLKQIDTELAKYPADQRRSAIMGALRIAQTEKGWLAPET
                     IAFVADYIGITPAQAYEVATFYNMYDLEPVGKYKLTVCTNLPCALRGGMATGEYLKQR
                     LGIGYGETTPDGKFTLVEGECMGACGDAPVMLVNNHSMCSFMTEEAIEKKLAELE"
     gene            complement(16370..17626)
                     /gene="nuoD"
                     /locus_tag="WIC03_000288"
     CDS             complement(16370..17626)
                     /gene="nuoD"
                     /locus_tag="WIC03_000288"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HEZ3882850.1"
                     /translation="MANKLRNYTINFGPQHPAAHGVLRMILELEGETIVRADPHIGLL
                     HRGTEKLAETKTYLQALPYMDRLDYVSMMVNEQAYCLAVEKLAGIDVPIRAQYIRVMF
                     AEVTRILNHLMGIGSHAFDIGAMTAILYAFRDREELMDLYEAVSGARMHAAYFRPGGV
                     YRDLPDFMPKYESSKFRNAKVLKQLNESREGTMLDFIDAFCERFPKNIDTLETLLTDN
                     RIWKQRTVGIGVVSPERAMQKGFTGVMLRGSGVEWDVRKTQPYEVYDKMDFDIPVGVN
                     GDCYDRYLCRMEEMRQSVRIIKQCSEWLRVNPGPVITTNHKFAPPKRTEMKTGMEDLI
                     HHFKLFTEGMHVPEGETYTAVEHPKGEFGVYIISDGANKPYRLKIRAPGFAHLQGMDE
                     MAKGHMLADVVAIIGTQDIVFGEVDR"
     gene            complement(17616..18209)
                     /locus_tag="WIC03_000289"
     CDS             complement(17616..18209)
                     /locus_tag="WIC03_000289"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEZ3882851.1"
                     /translation="MASIQNLYETVVGVLGDQASKVISALGEITVECLPEHYVSVMTT
                     LRDHEELHFELLVDLCGVDYSTYKNEVWQGKRFAVVSQLLSVKNNQRIRVRVWVSDDD
                     FPVVESVVDIYNSADWYEREAFDMYGIMFNNHPDLRRILTDYGFVGHPFRKDFPISGY
                     VEMRYDEEQKRVIYQPVTIEPREITPRIVREENYGGQ"
     gene            complement(18222..18704)
                     /locus_tag="WIC03_000290"
     CDS             complement(18222..18704)
                     /locus_tag="WIC03_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HEZ3882852.1"
                     /translation="MGIEGVLKKGFITTSADTVLNYMRTGSLWPVTFGLACCAVEMMH
                     AGMARYDLDRFGIIFRPSPRQADLMIVAGTLTNKMAPALRRVYDQLAEPRWVLSMGSC
                     ANGGGYYHYSYSVVRGADRVVPVDVYVPGCPPTAEALIYGLIQLQQKIKRTSTIARDE
                     "
     gene            complement(18695..19051)
                     /locus_tag="WIC03_000291"
     CDS             complement(18695..19051)
                     /locus_tag="WIC03_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEZ3882853.1"
                     /translation="MLSAYFPVFVFILVGLAASVLFILLGTILGPKRHYAEKDAPYEC
                     GFEAFENARMKFDVRYYLVAILFILFDLEVAFMLPWAVVFKDLGAYGFWSMLVFIVVL
                     TVGFVYEWKKGALEWE"
BASE COUNT         4914 a         5164 c         4856 g         4299 t
ORIGIN      
        1 aacaaagccg acctgcccga aaccgccctt tggcgcgaaa aactcaaatt ctacgaaacg
       61 ctgggttatc ccgtcatcga aacccgcgca ctggaaaacg ccggcagcct gcgccccgcc
      121 ctgcaagggc acagcaacat cctgctcggg cagagcggta tgggcaaatc caccctgacc
      181 aacgcccttt tgggcagcca aaccgcccgc accggcgaca tttccgccgc actcgactcg
      241 ggaaaacaca ccaccaccca cgcccggctt tatgatttga acggcgaaac ccaactcatc
      301 gactccccgg gtttgcaaga atttggttta caccacctcc aagccgccga tttgccgcgc
      361 tatttccccg atttccgcca ccttgtcggg caatgccgct tccacaactg cacccaccgc
      421 gccgaacccg gctgcgcctt caaagccgcc gcgcaaaccg gggcggcaag ccccgaacgc
      481 ctcgcctttt tgcagggcat caccgacgaa ctgcccgggt aacgccttgc cgcctgggcg
      541 gaaaaatgcc gtctgaagcc ggattcgggt ttcagacggc atccgttttt caaaaatgct
      601 acaatccgct ttttactgga acatccgaaa ctatgttcca acacgcagga cgacacttaa
      661 agcaccgccc tatgtgtcgt cctgatttgg aagggcttac acccctccca aataaagtct
      721 gattctaccg ccccgaagga cagatgtcca agtggcgggg tttcaaccga aaaggaaaca
      781 cgatgaagaa aaacgccccg aaatcgtttg aagaagcctt gtcgcgcctt gaatcgctga
      841 cacaagctat gcagggcgaa atgcccttgg aagacgcgct tgccgcctat caggaaggca
      901 acgagctggt cagatactgc caaaccaagc tggcgcaagt cgaacagaaa ttacaggttt
      961 tggacgcgga cgggccgaag gagttgaacc ttgaatccga cgaatgattt gaaagcgtgg
     1021 caacagaggg cgcaggcgca aacagagctg ctgctggaac gctttttgcc gtctgaaaac
     1081 gaaataccgc acacactgca cgaagccatg cgttatgcgg ttttggatgg gggcaaacgt
     1141 ctgcggccga tgctggttct ggcagcttcg gaattaggcg aagccgtgca tgaagcagta
     1201 gaacaggcaa tggcggcaat cgaaatgatc catgcctatt cgttggttca cgacgatatg
     1261 ccggcaatgg acaacgacag cctgcgtcgg ggcaaaccga cctgccacat caaatatggc
     1321 gaagccaccg cgctactggt cggcgacact ttgcaaaccc aagccttcga cgtgttaagc
     1381 cgtccgacag aactgcccgc agcacgccag ttggcaatgt tgtcggtgtt ggcgaaagcg
     1441 ggcggcagcg cgggcatggc gggcggtcag gcaatcgatt tggcgaatgt cggcaaacaa
     1501 atggttcaag ccgatttgga acagatgcac agcctgaaaa cgggtgcgct aatccgtgcg
     1561 gcggttttat tgggggcgat gtcctgcccg aatctgtctg aagacagtct ggcggtattg
     1621 gacgattatg cggcaaaact ggggctggcg ttccaagtca ttgacgatgt gttggattgt
     1681 gaagcggaca cggcgacttt gggcaagacg gcgggcaaag atgcagacaa cgacaagccg
     1741 acttatgtga aactgatggg cttggaagcg gcgcgctcat acgcacacaa actggttgcc
     1801 gaagcggtcg cgctgctcga acccttcggc gacaaagccc tgcgcctgcg gcagttggca
     1861 gaatttgcag tcgcccgcaa atattaaaac cggcgtatat cgccggctga aacgtgccgg
     1921 cacagcgatg ccggcctgca cttgaatgaa acaaatgccg tctgaacagg tttcagacgg
     1981 catttctatg cttgtctatt ccctgttgcg cgtgtgccaa aaataccgcc acacaaaaca
     2041 tggaaacgca aaaatcaggt acaggcagac gactgtggca taaaactccc aaccctgatc
     2101 gtgtaccgat cctgcacggg attcgagaat ataggcaaga atggcggtca gtgcgaaacc
     2161 tgccgccagc tcgatcatgt ggtgtccgaa atgtttgcgc ttgagcgcgg ccacgccgaa
     2221 cagtctggtg gtgaggaagg gggcgttggc aaagatgagt gccaagacca aaaggatgta
     2281 catggatgcg gtcatggtgg gctttctgtt tgggcattgt cggcggcgat gccgtctgaa
     2341 ctgcattgcg gaacggcata tcttaacaaa aacggcagcc tctgacacgg ctgccgttgc
     2401 ggcggcttac agcgtgttct ccaacgcctt ggcgcaccag tcgataacgg tttgcggcat
     2461 aatgccccac agcaccaaca gcagcgcgtt gatgcttaag aggaattttg cggcattgtt
     2521 gccgccggcg gcgggacggg cgcggccgga ttcgtcaaaa tacatgactt tgaccacgcg
     2581 caggtagtag aacgcgccaa tcagcgacat cacgacggca aatacggaca accaaacata
     2641 gccttgtttt aagagtgcca taatcacgcc gaatttggcg taaaagccca tcagcggcgg
     2701 aatgcccgcc atagagaaca taaccagcag catcaaaaag gcaagccata cgcggtgttg
     2761 gttcaaccct gccaaatcgc tgatgttttc gcactcgttg tccccgtccg acaacaccat
     2821 caacacaccg aaccctgccg ccgccatcag cgcgtaggta atggcgtaat agagacccgc
     2881 cgcaaagccg accgcgcccg ccataaacgc cagcaggata aaccccatat gcgatacggt
     2941 ggaataggcg agcatacgtt tgatattggt ctgcatgatg gcggcaaggt tgccgaccag
     3001 cagcgaggcg gcggcaagca gggcaaacat cagagaccag tcatgatgca cggttcccaa
     3061 cccggtaacg aggatgcgga aagcgaaaac aacggcggcg attttcgggg cagtgccgac
     3121 cagggcggta acagaagtgg gcgcgccgtg atacacgtcg ggcacccaca tatggaacgg
     3181 caccgcgccg agtttgaacg caacggcgac gacgataaac accagaccca gtttcaacag
     3241 ccattcgttt gcttcttcat tgaaggaaga ggcgagcacg ccggcaaatt ccagcgaacc
     3301 ggttgcgccg taaaccatag aaataccgta gagcagcagg ccggatgcca gcgcacccag
     3361 aacaaaatat ttcaaggcgg cttcggcggc aaagccggaa tcgcggcgca gggcaatcag
     3421 ggcgtaaagg gcaagcgaca agagttccaa accgatatag gcagttaaaa aatgccccgc
     3481 gctcaccatc acgcccatac ccaagagggc aaacagcgac agggtgtaaa actcgccttt
     3541 gaaaataccg cgcacttggt tgtagggctt ggcatagaca aacagggtaa acatcaaggc
     3601 atataaaacc atttttgcca aacgcgacat accgtctgca atatacatcc cgttgaacga
     3661 agacgtgctg ccctgttccc acaccgccaa ctgcaccaca gccgtaaccg ccaccgttgc
     3721 caacgcgccg taatgcgtcc accggcgttt gtcatcactg acccacaagt ccgccagcaa
     3781 caataacacc agcagcgcaa gcagcacgat ttcgggcatg gcgggcatta aattcaaatc
     3841 agaccagttc atttacacac ctcaaatctt gctttgtgcc acatgggcaa tcaaatcgtt
     3901 tgccgcctga tgcaccactt cgataaatgc gttcggatac aggcccatac ccaataccgc
     3961 aaccgccaaa accgccagaa tcgtaaactc gcgacagttg atgtctttca tttcggcaac
     4021 gtgcggattg tgaatcgcgc cgaaaatcac gcgcttgtac atccacaggg tataagacgc
     4081 accgtaaatc agcgtcatag ccgccaacgc gcccacccag aaattcactt taaccgcgcc
     4141 cataatcacc ataaactcgc ccacgaagcc agaagtcgcc ggcagaccgg cattggccat
     4201 accgaacaac atcataaacg ccgcaaactt aggcatcaca ttgaccacgc cgccataatc
     4261 ggcaatattg cgggtatgca ggcggtcgta catcacgccg atacacataa acatcgcggc
     4321 agacacaaaa ccgtgggaaa tcatttgaat gattgcgcct ttcaatgccc aatcattcaa
     4381 ttggccgttc acaaacagga acatacccaa agtgacaaaa cccatatggc tgatggacga
     4441 atacgccacc agttttttca tatcggtttg caccaaagcc accataccga tgtaaatcac
     4501 ggcaatcagg ctcaatacaa tgatcacagg ggcaaaatag cgtgccgcat ccggcataat
     4561 cggcaggata aagcgcaaga aaccatacgc acccagcttc agcgtaatcg ccgccaacac
     4621 catcgaaccg ccggtcggcg cttcaacgtg ggcatccggc aaccaagtgt gcacagggaa
     4681 catcggcact tttacggcaa atgacaggaa gaacgctgca aataaaagct gttgtacgcc
     4741 caacggaatc tgtttgatgt tttggaaatc gacaatagag aagctgcctg tttgatagta
     4801 caggtaaacc atagcaacca gcatcaggag cgaacccatc agcgtataga ggaacagctt
     4861 gaccgacgca tagacgcggc gcgggccgcc ccatacaccg ataatcaggt acagcggaat
     4921 cagcataccc tcgaagaaca cataaaacag aatcgcatcc tgcgcggcaa acgcaccgtt
     4981 aatcaaaccc gacatcatca ggaatgccgc catatactgc gccgggcgtt tctgaataac
     5041 ttcccaaccc gccagcacca ccaacagcgt aataaacgca ttcaagatga taaagagcac
     5101 tgaaataccg tccacgccca atgcatagtt gattttcagc agcggaatcc actcgtggaa
     5161 ctcggtaaat tgatagccgc cgctcaaacg gtcgaaaccg gtaaacaggg gcagcgttac
     5221 caagaaaccg gcaagcgcac ccataaaggc aagcacacgc gccagcggcg cacggctgtc
     5281 cttaccggtc gacaaaacca gcacacctgc ggcgatgggt atccatattg ccaagctgag
     5341 taggtagttg gaaaacatag tggttaacct gtggttaaaa taaaaatatc gttgtacggg
     5401 ttggtttgtc agacgacgtt ttaagagacc gtctgaaacc tcatccttat ctgaacaatc
     5461 cccagaaggt cataccgagc aagaccaata cgccgaacac catagcggcg gcgtaggtat
     5521 agataaagcc ggtttgagct ttacgcactt gcgcggcaat cgcgccgacc agtttggcag
     5581 agccgttgac gatgccgttg tcgataatgg cggtatcgcc gactttccag aagaaagtac
     5641 ccaatgcgcg tgtgcctttg gcgaaaacgt tgaaatacag ggcgtcgagg tagtatttgt
     5701 tttcaaacaa aacgtaaatc ggacggaacg tctgtgcaat tttcgctggc aggtgcggca
     5761 gtttgacgta caaaagccat gcgctcaaca cgcctgcgat agcaaggtag agtacaggcg
     5821 aatgcaggct gtgcgacacc attgccaatg cgccgtggaa ctcttccttc atgatgtgca
     5881 tggtcggatg cgcgtcggcg ttgacgaaaa tcacatcttt gaagaaatcg ccgtaaagca
     5941 taggctcgat ggcaacgtag ccgatgatga cggacggaat ggcaagcaaa atcaaaggca
     6001 gggtaacaac caacgggctt tcgtgcggat tgtcgttttt gcccaagctg tgatgttctt
     6061 cgccatgatg atcgtcatga tgttcaggca agctgcgcca tttctcttcg ccgtggaaca
     6121 ccataaagta ttggcggaac gcgtagaacg cggtaacgaa cacgctggca aggacggcaa
     6181 aataggcaaa gccgctgccc ggcagggtgc tgtatttcgc cgcttcgata atcgaatctt
     6241 tagaatagaa gccggagaag aacggcgtac cgatcagcga caagttaccg atcagcatgg
     6301 tcagccaagt aatcggcatg tattttttca ggttgcccat gtggcgcatg tcttggtcgt
     6361 ggtgcatacc gataatcgcg ctgcctgccg ccaagaacaa caaggcttta aagaaagcgt
     6421 gggtcatcac atggaacatc gccacggaat aggcagacgc acccaaagcc acggtcatat
     6481 agcccagttg cgacaaggtg gaatacgcaa ccacacgttt gatgtcgttt tgaatcacgc
     6541 ccaagaaacc cataaacagg gcggtaatcg caccgatcac cataatgaca gacagcgcag
     6601 tgctgctcat ctcataaatc ggcgacatac gcgataccat aaacagaccg gcggtaacca
     6661 tcgtcgcagc gtgaatcaat gcagaaatcg gggtcgggcc ttccatcgaa tcaggcagcc
     6721 aaacgtgcag tgggaattgt gccgatttac ccatcgcacc gacaaacagg agcaaacagg
     6781 tgacagtaat caacgaccat tccacaccgg ggaaaagctg gatggtggca ttttgcacgt
     6841 tgggcagata agcgaataca tcttgatagc gcaggctgcc gccgaaatag gcaagcacca
     6901 agccgatacc gagcaaaaag ccgaagtcgc cgacacggtt gatcaaaaag gctttcaggt
     6961 tggcaaatgt tgcgcttgga cgtttgaaat agaaaccgat caagagatac gacaccaagc
     7021 ccacagcctc ccagccgaag aagagctgga tgaagttgtt gctcataatc agcatcaaca
     7081 tgctgaaagt aaacaaagaa atatagctga agaagcgttg gtagccgact ttttcatcgt
     7141 gcatatagcc gatggtgtag atatgcacca tcaacgatac gcccgttacc acgaccatca
     7201 tcatcgccgt catcgtatcg accaagaagc cgacggagaa atccaaaccg cccatcgtca
     7261 gccaagtata gacgttctca tcaaacttgg cacggctgcc attgaggaat ccccacagca
     7321 cataagccga cagcacggca gacaccgcca caccgagtat cgtaacagta tgcgcaccgg
     7381 cacgaccgat tttgttgccg aacaaacccg caatcaacga gcctgccaac ggaacaaggg
     7441 caattatcaa atataaagtc atgtcgttca tttgattgaa tccgattgat ttaaaaatct
     7501 atgtttgttt cgtacaaaat tacttcggaa aaacaaatcc aacacgctcc aatcgtttgc
     7561 gtgccacagc taattgctct tcagtaaata aatcacacca cggcttttgt aacaccagat
     7621 attccatact gtatttcaag gcgtggactc cgccaccact cagaatcagc tctgtaaaac
     7681 cggtctgagt cttcttttcc cccgtactta ataatttatc cgccgcctct ttaccaccaa
     7741 attcatttac aatttgtaaa aatcgtgtcg ccttgtaagg ttgcggcaaa ttcaaagcct
     7801 cctgataaat atttaacatg gctttatgaa attcttgttc taactgattt ttatccatca
     7861 ttcttcttcc aatatttcag accggattat tcttacccag aatttctttt ctcatccgct
     7921 cccgtctgat cacctaccga atcaggtcgt ctgaaacagt ctgaaatcgc ttttcagacg
     7981 accctcagcc tttttcatac ccttcgtaat aatacgactg ctcgatacct ttaaagatga
     8041 tttcacggtt gtccacatcg tcggtcaggt tgtcctttaa cagaaagcgc agttctaaat
     8101 cgttgacggg gctgcgttcc atcgcctgca aatacagggt tttgcttaca ttttgccagt
     8161 ttacgatttt tttcaggttt ttcttcagca ccaaatccag ccagatgcgg gtacttctgc
     8221 cattaccctc caaaaacgga tgggcaatgt tcatttcaac atatttggcg atgatttctt
     8281 caaaagtccg ctcgggcatc tgctcgattt taaccaaagc ctcttttaaa tacatggcgt
     8341 tggcaaaacg aaaaccgcct ttggaaatgt tgtcttccct gatttgaccc gcaaaatcat
     8401 ataagccgcc gaacaggtaa cggtgaatct gttgcaggcc cgcggtggta ccgacttcga
     8461 tacggtcgat gtcgccgctt tcaaacaggc ggcgggcatt atgcaggctt tgttcgtcta
     8521 tggatttcat cgtttttcct atcgggtttt cagacggcat cggtgtcttg tcaggtctcg
     8581 acccaaccta cctttaccct ttcaactcgt ccaaatcggc aacattaatc gtttgtcggt
     8641 tgcggtacac caataccata atcgccaaac cgatggcaga ttcggcagcg gcaacggtca
     8701 atacgaagaa tacgaaaatt tgtccggcag tatcgcccaa atgttgcgag aaggcgataa
     8761 agttgaagtt caccgccaag agcatcagct cgatggacat cagcaatacc agcacgtttt
     8821 tgcagttcat aaagataccc attgcgctga taccgaacag gagcgcaccc aataccagat
     8881 aatgcgtcaa ggtaatcatg ctttgccctc cccttccggc ttgaggccgt ctgaaacttc
     8941 gctctcttcg gcagattcga cttgcggttt gaccgcttcc attttcacca gacgcatacg
     9001 gccttgatcg gcgcgtactt taacttggtc ggcaggatcc atgcgtttcg gattggtggt
     9061 tttacggtaa accagcgcaa tcgccgccac catacccaac agcagcaata ccgccgccaa
     9121 ttcaaacggc aacaggtagt cggtataaat acggctgccc aaatcgcgga tattgttgta
     9181 atcggccgga atgtctttca tcagaccaaa tgcggccagg tcggttttcg ggttgaccag
     9241 aatcaggatc agggcaaccg ccaacaatgt gccgaccaca ccggcaaccg gcgcatgacg
     9301 ccagaaaccg gcgcgcattt cttcaatgtc gatgttcaac atcatcacaa cgaacaggaa
     9361 caacaccatc acggcgccga cgtaaaccac caccagcgtc acgcccaaaa actcggcctg
     9421 catcagcatc caaagcatcg cgctcacgca gaaggtcagc accagatgca aagcggcgtg
     9481 gacagggttt ttggcggtaa cggtacgaac cgcaccgtac aaaacgatgg cggcaagaat
     9541 atagaacaga atcgcggaaa aagtcattcg tctgctcctt aacgatacgg cgcgtcagcg
     9601 gctttgcgtt tggcgatttc agcttcgtat ttgtcgccaa tggccaaaag aatcggcttg
     9661 gtcatgtgca agtcgccttt tttctcgccg tggtattcaa aaatatgggt ttccacaatc
     9721 gcatcggtcg ggcaggcttc ttcgcagaaa ccgcagaaga tgcacttggt caggtcgatg
     9781 tcgtaacgct tggtgcggcg cgtgccgtct tcgcgttctt ccgattcgat gttgatcgcc
     9841 attgcggggc aaacggcttc acacaattta cacgcgatgc agcgttcctc gccgttcgga
     9901 taacggcgtt gcgcgtgcag accgcggaaa cgcacggatt gcggcgtttt ctcttcggga
     9961 aaataaattg tgtctttgcg ggcaaaaaag tttttgagcg ttacgcccat gccttttacc
    10021 aactcgccga gcagaaaggt ttttactaag ttagccatat tatgttccct caaaacaggg
    10081 atttcgttag gtattcaaaa tcgctttgtt cagacggcct caagatgccg tctgaaactt
    10141 atttccacaa attcagcggt gaaatcatcc acacgcccaa aatcacgatg taggcgaagc
    10201 cgatcggaat cagcactttc cagcccaaac gcatgatttg gtcgtaacgg tagcgcggga
    10261 aggttgcacg gatccacaga taccagtaca acactgccgc cattttcaca aacatccaga
    10321 atgcggaagg cgtaccgata aagccccagc tttgcgggaa cggagacaac cagccgccga
    10381 gaaacatcag cgatgtcagc gcggcaatca gaatcatgaa aatgtattcg gcaaggaaga
    10441 acagcgcgaa tgcgaagccg gaatattcaa cgtggtgtcc ggcaacgatt tcggactcgc
    10501 cctctgccac gtcaaacggt gcgcggttgg tttcggcaac ggcggaaatc agatagacga
    10561 tgaagattgg gaacagcggc agccagttcc aagagaatac cgaaccgccc gcgatacctt
    10621 ttgcctgcgc ggcaacgatg tcggagaagt tcatgctgcc cgacaccatc acgacacaca
    10681 ccagcgcggc actcatggca atctcgtagg aaatgctttg tgcggaagca cgcattgcac
    10741 ccaagaacga atatttggag ttggaagccc agcccgcgat gatcacgccg taaaccgaca
    10801 gcgaggtaat catcaggatg tacaaaaggc cgatattgat gttggtcagc acccattctt
    10861 cgttgaacgg aatcactgcc cacgccgcga aagacggggc gagcgacata atcgggccga
    10921 tgtagaacag ggctttgttt gacagcttcg gacgggttac ctctttaaac aagagtttga
    10981 acacgtcggc aaacggctga atcagacccc acgggccggt tacgttcggg ccgacacgaa
    11041 gctgcataaa gccgatgact ttacgttcga aatacgtcag gtaggcaacg gtcagaatca
    11101 gcggaatcag gataatcacg attttgacga tgacggatac caccaaacct acggtaatac
    11161 ccaaatcgcc cagaccgagc gttgcggcaa agaggttttg gaaccattcc tgcataatca
    11221 agctcccgcc agttcaatag tgtccatcaa cgcacccagc gcggcatttt cggtatgcag
    11281 cggcagatgc accacgtttt caggcaaacc ggcatcggct ttgacggcaa ccgatacgct
    11341 tgcgccgttt tgtttggcga cagcagtttg tccgttttgc aggcccaagc gtgccaaagt
    11401 gttcggattg acacgcgcgg cgggcacggc ggcatggccg gtttcttgca acggtgcgga
    11461 acggcgcacg atagaatcag tgtgataaat accgacgccg ccgacacgga cgaggcggtc
    11521 tgaggtcgtc tgaacgccct cccctgtcca tgtgttgcgg ttgtccagtt tggacggcag
    11581 gctttctaca tccagcgcat ctttcagaat cgcagcggta tcgtggtatt caaaaccttg
    11641 caggtcaaac aggttgccca atacgcgcaa tactttccac atcggacgcg aatcgccgaa
    11701 gccttgtacc acgccgtgga aggattgcag acggccttcc atattgatga agctgcccga
    11761 ggtttcggta aacggcgcaa tcggcaacaa cacgtcgcac acatccagca gcgtttcgct
    11821 gacaaacggt gtaaacgcca tcacgctttt cgcctgtttc aacgcggcca cggctttcgc
    11881 accgtccgcc gtatcgattt ccggttcgac gttgagtagc aagaccgcct gtttcggcgc
    11941 gtttgccatt tcggcaacgc tctcgcccga gttcacgccc aagacatccg caccaacgct
    12001 gttggcggct tgcggcaaaa tacccagcac tgcgccggtc gcggctgcca gctcttgcgc
    12061 ggcagcataa atggcggcgt aatcaggatg gttttgcact tccgcgccca aaatcaccgc
    12121 tgctttttca gcatttttca ggctggcggt aacggcgtgt tccgcattgg cagacaggtt
    12181 tttcagacgg cctgcccact cgtcgggatg tgcggcttct tgagagagaa gcggcatcaa
    12241 caactcttcc ttgctgccgg ccaatacgct caaggccata cggtctttgg cggcgcggcg
    12301 caggcgggcg gtcaggagcg gctgttcttt gcgcaagttc gcaccgacta ccaatacggc
    12361 atcgttgtca gccaaagatt caatgctttg tcccaaccat tgtgcacctt taaggtcgtc
    12421 tgaaagacgt ttgtcttgtt ggcgcagacg ggtggcaaag tttttaacac ccaaaccgtc
    12481 ggcgagtttt ttggccagat acagctcttc aaccgtattc atcgggttcg cccaaacgcc
    12541 gacttggttt tgattgccgt ctttggcgat acattcaatc gcgctgcgga catattccaa
    12601 cgcggttttc caatccacgt ccatccactc gccgccctgt ttgattttcg ggtttttcag
    12661 acggctttcg tgatacaggc cttcgtaggc gaaacggtcg cggtcggaca gccagcattc
    12721 gttgatggct tcattttcca gtggcaacac gcggcggacg gtatggtctt tggtttgtac
    12781 aatcaggttg ctgcccaaag cgtcgtgggc ggaaacggat ttgcggcggt tcaattccca
    12841 agtacgcgca ttgaagcgga acggtttgct ggtcagcgcg ccgacgggac acaaatcaat
    12901 gacgttgccc gacaattcgg tttccaccgt tttgccgata aagggcatga tttcggagtg
    12961 ttcgccgcga ttcaccatcg caatttcctg caaaccggcg atttcttcag tgaaacgaac
    13021 gcagcgggtg cagtggatac agcggctcat ttcctcggcg gaaaccaagg accccatatc
    13081 tttgccgacg acggaacgtt tttcttcggt gtagcggctg gtggttttgc cgtagcccac
    13141 cgccaaatcc tgcaactggc attcgccgcc ttggtcgcag gtcggacaat caagcggatg
    13201 gttgatgagc aggaactcca tcacgccttc ctgcgcctct cgggcttttg ccgaatgcgt
    13261 acgcacaatc atgccgtctg taaccggcgt ggcacaggca ggcaggggtt ttggggcttt
    13321 ttctacgttc accagacaca tacggcagtt ggcggcgatg gaaagttttt tgtggtaaca
    13381 gaaatgcgga atataagtac cgagcttgtg cgcggcttca atcaccgtcg cgccctgctc
    13441 cacagatact tgtttgccgt cgatttcgat ttgtaacatg gttcgttcct agttacgggt
    13501 atttgataaa taatctttta tggaggtcgt ctgaaaaatg ggtttcagac ggccttttga
    13561 gtttcattga caatcaatac tgtttaaaac gtttactgcc accttgccgt cattcccgcg
    13621 taggcgggaa tccatttttt gaatttcggc aactgctttt caaatatcgg gttctgtaaa
    13681 ttccactatg gattcccgcc tacgcgggaa tgacggcaaa gttaaatttt tagcattttg
    13741 cttttaacca atataaaacc aactaaaact ataacttatt tattatattt ttaaatgact
    13801 taaataactc tttaacagca cgctcattgt cttcatctgt aggctcaaac ttttcacacc
    13861 aattaattat atttacatcg ccatgcaata actctgcata acatcctact gcatcgcaac
    13921 taccaccccg tgtccttgac gtgccaaaaa taaaatcaca attatgcgaa taaagtttgt
    13981 ataaatttct cctaacctcg tattcattat caccagatga ataaaaacca atcctctgac
    14041 cttcatgatc aaatactgca acaaaatcta agccgtttcc atgtctttca aaaaagacta
    14101 gaaatttaga aaatttccga caaatctgat taaaaagcgt attgagtgtc gtactcttac
    14161 ctttgttggc tgcaccgtat agaataaaaa ttttagcttc caccattacc accctcccca
    14221 cttatgctct ttcatcggcc cgccgtgttc gatgtaatgc acaaactcat cacggaaatg
    14281 cttggtaaag ctgcgaacag ggaaaacggc ggcatcggaa agggcgcaga tggtgcggcc
    14341 tgccatttgg ttgccgacgg aatccagcaa atccaaatct tccattttgc ctttgccttc
    14401 tacgatgcgg tggacgatgc ggtagagcca gcccgtacct tctcggcaag gcgtacattg
    14461 gccgcaagac tcgtcgtagt agaagtagct cagacgctca agggctttca ccatgcatac
    14521 gtcttcatcc atcacgataa tcgcgccgga acccagcatc gagcccgctt tggaaatcga
    14581 gtcgtagtcc atattggtct gcatcatgat gtcggcaggc aaaactggcg cggacgaacc
    14641 gccgggaata acagctttga gttttttacc gccgcgcata ccgcccgcca ttttcaagac
    14701 ttcggcaaac ggcgtgccca acggcacttc atagttgccc ggacgctcga catggccgga
    14761 aatacagaac aatttggtac cgcctgcatt cggaatacct ttatcggcaa atgcctgtcc
    14821 gccgtcacgg ataatgaatg ggacggagga gaacgtttcg gtattgttga tggtggtcgg
    14881 tttgccgtac aggccgaacg aagcagggaa tggcggctta aagcgcggct ggcctttttt
    14941 gccttccagc gattcgagca atgcggtttc ctcgccgcaa atatatgcgc cgtagccgtg
    15001 gtgggcgaag agttcaaatt caaaatccga acccaaaata tttttaccca aaaagcctgc
    15061 ggcacgcgcc tgctccaaag cggcctcaaa gcgttggtag ccttcaaaaa tttcgccgtg
    15121 gatatagttg taaccggctt tcgcgcccat cgcgtaaccg gcgataatca tgccttcgat
    15181 cagggcatgg ggattgaaca tgatgatgtc gcggtcttta aatgtacccg gctcgccttc
    15241 gtcggtgttg caaacgacgt atttttcgcc cggaaaggaa cggggcataa agctccattt
    15301 caaaccggtc gggaagcctg caccgccacg accgcgcaag ccggaggttt tgacttcgtc
    15361 aatcacatcg gtttgagaga tgtttccgga caggatttta cgcagggcgg tatagccgcc
    15421 gcgtttgacg tattcgtcca atgtccagca gtcgggattg gcggtatcca cttggtcaaa
    15481 aatcacgcct gattggtaaa tagccatttt tggtgtgcct gtttgttttc gtatcggttg
    15541 cggccgctgt ttcagacgac cttaagatgt ctttgtgtac tggcttgtaa cgtcgtctga
    15601 aataaaatct agtttatcaa atcgatattt tcagacgacc tttaatcgtt tttgttggat
    15661 ctcgaccact tgcttatctg tcgtcattcc cgcgcaggcg ggaatccatc ctcaatggta
    15721 agcaatgtct tattaaattc agaaaccgaa tcttaccggt ggattcccgc ctgcgcggga
    15781 atgacggcat ttcggtattt cagtagggcg gattcttaaa tccgacattt tgccttttta
    15841 cccactctgt cggatacaag tatccgacct acgtttaaat cgtcgtttca gacgacctac
    15901 tccaactccg ccagtttctt ctcaatcgcc tcttcagtca taaagctgca catgctgtgg
    15961 ttgttgacca gcataacggg ggcatcaccg cacgcgccca tgcattcgcc ttcgacaagg
    16021 gtaaatttgc cgtcgggcgt ggtttcgccg tagccgatac cgagtctttg tttgaggtat
    16081 tcgccggtcg ccataccgcc gcgcagggcg cagggcaggt tggtacaaac ggtcagtttg
    16141 tatttgccga caggctcaag atcgtacata ttgtagaagg tagcgacttc gtaggcttgt
    16201 gcaggcgtga tgccgatata gtcggcaaca aaagcgatgg tttcgggggc gagccagcct
    16261 ttttcggttt gggcgatacg caacgcaccc atgatggcgg aacggcgttg gtcggcggga
    16321 tatttcgcca gttctgtgtc gatttgtttt aaggactctg cggataacat tatcggtcaa
    16381 cctccccgaa tacgatgtcc tgcgtaccga tgatggcaac gacgtcggcg agcatgtggc
    16441 cttttgccat ttcgtccata ccttgcaggt gggcgaagcc gggtgcgcgg attttcaggc
    16501 ggtagggttt gtttgcgccg tctgaaatga tgtaaacgcc gaactcgcct ttcgggtgtt
    16561 cgacagcagt gtaggtctcg ccctcgggaa cgtgcatacc ctcggtaaat agtttgaaat
    16621 ggtgaatcag gtcttccata cctgttttca tttcggtacg tttgggcgga gcgaatttgt
    16681 ggtttgtggt aatgaccgga cccggattga cacgcaacca ctcggagcat tgtttgatga
    16741 tgcgtacgga ttggcgcatt tcttccatac ggcagaggta gcggtcgtag cagtcgccgt
    16801 tcacgccgac agggatgtcg aaatccattt tgtcgtacac ttcgtaaggc tgtgtcttac
    16861 gcacgtccca ttccacgccc gaaccgcgca acatcacgcc ggtaaagcct ttttgcatgg
    16921 cacgttcggg ggagacgacg ccgatgccga cggtacgctg tttccaaata cggttgtcgg
    16981 tcaggagggt ttcgagtgtg tcgatgtttt tcgggaaacg ttcgcagaag gcatcgataa
    17041 agtcgagcat cgtgccttcg cgggattcgt tgagctgctt caatactttg gcattgcgga
    17101 atttgctgct ctcgtatttg ggcatgaagt cgggcaggtc gcggtaaaca ccgccgggac
    17161 ggaagtaggc tgcgtgcata cgcgcgccgg acacggcttc gtacaagtcc atcagctctt
    17221 cgcggtcgcg gaaggcgtag aggatggcgg tcatcgcacc aatgtcgaat gcgtgcgaac
    17281 cgatgcccat caagtgattg aggatgcgcg ttacttcggc gaacatcacg cggatgtatt
    17341 gggcgcggat aggtacatca attccggcga gtttttctac cgccaagcag tatgcctgct
    17401 cgttgaccat catggaaacg tagtccagcc tgtccatata aggcagggct tgcagatagg
    17461 ttttggtttc cgccagtttt tcggtgccgc gatgcaggag accgatatgc gggtcggcac
    17521 ggacgatggt ttcgccttcc aactccaaaa tcatacgcaa tacgccgtgc gccgcagggt
    17581 gttgcgggcc gaagttgatg gtgtagtttc ttaatttatt ggccaccgta gttctcctca
    17641 cggacgatac gcggcgtgat ctcgcgcggc tcaatggtaa caggttggta aatcacgcgt
    17701 ttttgctctt cgtcgtaacg catttccaca tagccggaaa tcgggaagtc tttgcggaac
    17761 ggatgtccga cgaaaccgta atcggtcagg atgcggcgca agtccggatg gttgttgaac
    17821 atgatgccgt acatatcgaa ggcttcgcgt tcgtaccaat ccgcgctgtt gtaaatatcg
    17881 actacagatt cgactacggg gaagtcgtcg tctgaaaccc agacgcgcac gcggatgcgt
    17941 tgattgtttt taacggaaag caactgactg acgacggcaa agcgtttgcc ctgccatact
    18001 tcgtttttgt aagtgctgta atcgacgccg cacaagtcaa ctagaagctc gaaatgcaac
    18061 tcttcatggt cgcgcaacgt agtcatcact gaaacatagt gttcgggcag acactcaacg
    18121 gtaatctcgc ccaaagcaga aatgactttg cttgcctgat cgccaagcac gccgacaacg
    18181 gtctcgtata agttttgaat gcttgccata tcgtcctctc cttactcgtc acgcgcgatg
    18241 gtggaagtgc gcttgatttt ttgttggagc tgaatcaggc cgtaaatcag ggcttccgca
    18301 gtcggcggac aacccggcac ataaacgtct accggcacga cgcggtcggc accgcgcaca
    18361 acggaataag aatagtgata atagccgccg ccgttggcac acgagcccat agacaatacc
    18421 cagcgcggct cggcgagctg gtcatacacg cggcgcaggg cgggtgccat tttgttggtc
    18481 agcgtacccg ccacaatcat caggtcggcc tgacgtgggg acggacggaa aatgatgccg
    18541 aaacggtcaa ggtcataacg cgccataccc gcgtgcatca tttccacggc gcagcaggcc
    18601 aagccgaaag taaccggcca caacgaaccg gtacgcatat agttcaacac cgtatccgcg
    18661 ctggtggtga tgaaaccttt tttcaaaacg ccttctattc ccattccagc gcaccttttt
    18721 tccattcgta aacaaagcct accgtcagaa cgacgataaa caccagcata gaccagaagc
    18781 cgtacgcgcc caaatctttg aacacgactg cccacggcaa catgaacgcg acctccaaat
    18841 caaacaagat aaagaggatg gcgacgaggt aatagcgcac gtcgaacttc atcctggcgt
    18901 tttcaaaggc ttcaaaaccg cattcgtaag gcgcgtcttt ttcggcgtag tggcgtttcg
    18961 ggcctaaaat cgtgccgagc aggataaaca gcacgctggc cgcgaggccg acgaggataa
    19021 agacaaagac gggaaagtaa gcggacaaca tggttacacc caaatccgtt aacaaaattt
    19081 ctacaataat ttcgtatttt agcgaatttc aaaaaccatt aaaaggtaaa tatcggcaaa
    19141 acgcccaaaa aaaccccaat aaatacaatc atgttatgat aacgattctt atttgatttt
    19201 ataaggtcat ataaattttt acaccgtaat tcg
//
LOCUS       DAWHSK010000011        19123 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.011, whole genome shotgun sequence.
ACCESSION   DAWHSK010000011 DAWHSK010000000
VERSION     DAWHSK010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19123
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.011"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..485)
                     /locus_tag="WIC03_000292"
     CDS             complement(<1..485)
                     /locus_tag="WIC03_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FrpC operon protein"
                     /protein_id="HEZ3882854.1"
                     /translation="MRPYATTIYQLFILFIGSVFTMTSCEPVNEQTSFNNPEPMTGFE
                     HTVTFDFQGTKMVIPYGYLARYTQDNATKWLSDMPGQDAYSINLIEISVYYKKTDQGW
                     VLEPYNQQNKAHFIQFLRDGLDSVDDIVIRKDACSLSTTMGERLLTYGVKKMPSAYPE
                     Y"
     gene            complement(872..1147)
                     /locus_tag="WIC03_000293"
     CDS             complement(872..1147)
                     /locus_tag="WIC03_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEZ3882855.1"
                     /translation="MNASNWSVYLILCENSAFYCGISPNPQQRLAAHTTGKGAKYTRV
                     FKPVAMRIVAGGMDKGTALRQEIAVKKLTAAQKRQLWEQAEKMPSET"
     gene            complement(1144..2427)
                     /locus_tag="WIC03_000294"
     CDS             complement(1144..2427)
                     /locus_tag="WIC03_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ3882856.1"
                     /translation="MTASKSGFIGQIFSRNMLVCIFTGFTSGLPLYFLINLIPAWLRS
                     EQVDLKSIGLMALIGLPFTWKFLWSPLMDAVRLPVLGRRRGWMLLTQAGLLAALAAYA
                     FLNPRNHLPLIAGLSVLVAFFSASQDIVLDAFRREILSDEELGLGNSVHVNAYRIAAL
                     IPGSLSLVLADRMPWSEVFVITSLFMLPGLLMTLFLAREPVLPPAVPKTLKQTVVEPF
                     KEFFTRKGIASAVCVLLFIFLYKLGDSMATALATPFYLDMGFSKTDIGLIAKNAGLWP
                     AVAAGILGGVWMLKIGVNKALWIFGAVQAVTVLGFVWLAGFGHFDTVGTGERLMLAAV
                     IGAEAVGVGLGTAAFVSYMARETNPAFTATQLALFTSLSAVPRTVINSFAGYLIEWLG
                     YVPFFQLCFILALPGMLLLLKVAPWNGEKTQDAGR"
     gene            complement(2583..4001)
                     /gene="glnA"
                     /locus_tag="WIC03_000295"
     CDS             complement(2583..4001)
                     /gene="glnA"
                     /locus_tag="WIC03_000295"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEZ3882857.1"
                     /translation="MSIKNVVKLIEESEARFVDLRFTDTKGKQHHFTVPARIVLEDPE
                     EWFENGQAFDGSSIGGWKGIQASDMQLRPDASTAFVDPFYDDVTVVITCDVIDPADGQ
                     GYDRDPRSIARRAEAYLKSSGIGDTAYFGPEPEFFVFDGVEFETDMHKTRYEITSESG
                     AWASGLHMDGQNTGHRPAVKGGYAPVAPIDCGQDLRSAMVNILEELGIEVEVHHSEVG
                     TGSQMEIGTRFATLVKRADQTQDMKYVIQNVAHNFGKTATFMPKPIMGDNGSGMHVHQ
                     SIWKDGQNLFAGDGYAGLSDTALYYIGGIIKHAKALNAITNPSTNSYKRLVPHFEAPT
                     KLAYSAKNRSASIRIPSVNSSKARRIEARFPDPTANPYLAFAALLMAGLDGIQNKIHP
                     GDPADKNLYDLPPEEDALVPTVCASLEEALAALKADHEFLLRGGVFSKDWIDSYIAFK
                     EEDVRRIRMAPHPLEFEMYYSL"
     gene            4305..5114
                     /gene="aroE"
                     /locus_tag="WIC03_000296"
     CDS             4305..5114
                     /gene="aroE"
                     /locus_tag="WIC03_000296"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEZ3882858.1"
                     /translation="MTALPRYSVFGNPVAHSKSPQIHRQFALQEGVDIEYGRICADIG
                     GFAQAVSTFFETGGCGANVTVPFKQEAFHLADEHSDRALAAGAVNTLILLKNGKLRGD
                     NTDGIGLANDITQVKNIAIEGKTILLLGAGGAVRGVIPVLKEHRPARIVIANRTHAKA
                     EELAQLFGIEAVPMADVNGGFDIIINGTSGGLSGQLPAVNPEIFRDCRLAYDMVYGEA
                     AKPFLDFARQSGAKQTADGLGMLVGQAAASYALWRGFTPNIRPVIEYMKAM"
     gene            5116..5817
                     /gene="mtgA"
                     /locus_tag="WIC03_000297"
     CDS             5116..5817
                     /gene="mtgA"
                     /locus_tag="WIC03_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HEZ3882859.1"
                     /translation="MFRIVKWLIALPVGIFIFFNAYVYGNIITYRAVAPHRTAFMSMR
                     MKQFEQEGRDVALDYRWVPYNRISVNLKKALIASEDARFAGHGGFDWGGIQNAIRRNR
                     NSGKVKAGGSTISQQLAKNLFLNESRSYIRKGEEAAITAMMEAVTDKDRIFELYLNSI
                     EWHYGIFGAEAASRYFYQIPAAKLSKQQAAKLTARVPAPLYYADHPKSKRLRNKTNIV
                     LRRMGSAELPESDTD"
     gene            complement(6020..6095)
                     /locus_tag="WIC03_000298"
     tRNA            complement(6020..6095)
                     /locus_tag="WIC03_000298"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6060..6062),aa:Phe,seq:gaa)
     gene            complement(6286..7020)
                     /gene="lptB"
                     /locus_tag="WIC03_000299"
     CDS             complement(6286..7020)
                     /gene="lptB"
                     /locus_tag="WIC03_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEZ3882860.1"
                     /translation="MSANVSRLVVQNLQKSFKKRQVVKSFSLEIESGEVIGLLGPNGA
                     GKTTSFYMIVGLIAADAGSVMLDGQELRHLPIHERARLGVGYLPQEASIFRKMTVEQN
                     IRAILEIRTKDKNQIDREIEKLLADLNIERLRNNPAPSLSGGERRRVEIARVLAMKPH
                     FILLDEPFAGVDPIAVIDIQKIIGFLKSRGIGVLITDHNVRETLSICDRAYIISDGTV
                     LASGKPDDLVGNEQVRSVYLGENFKY"
     gene            complement(7094..7624)
                     /gene="lptA"
                     /locus_tag="WIC03_000300"
     CDS             complement(7094..7624)
                     /gene="lptA"
                     /locus_tag="WIC03_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HEZ3882861.1"
                     /translation="MIQKICKLFVLIAFFSASPAFALQSDSRQPIQIEADQGSLDQAN
                     QSTTFSGNVVIRQGTLNISAARVNVTRGGKGGESVRAEGSPVRFSQTLDGGKGTVRGQ
                     ANNVAYSSAGSTVVLTGNAKVQRGGDVAEGAVITYNTKTEVYTISGSTKSGAKSASKS
                     GRVSVVIQPSSTQKSE"
     gene            complement(7605..8186)
                     /gene="lptC"
                     /locus_tag="WIC03_000301"
     CDS             complement(7605..8186)
                     /gene="lptC"
                     /locus_tag="WIC03_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEZ3882862.1"
                     /translation="MKIRWRYGIAFPLILAVALGGLSAWLGRISEVEIEEVRLNPDEP
                     QYTMDGLDGRRFDEQGYLKEHLSAKGAKQFPESSDIHFDSPHLVFFQEGRLLYEVGSD
                     EAVYHTENKQVLFKNNVVLTKTADGKRQAGKVKTEKLHVDTESQYAQTDTPVSFQYGA
                     SHGQAGGMTYDHKTGLLNFSSKVKATIYDTKDM"
     gene            complement(8183..8719)
                     /locus_tag="WIC03_000302"
     CDS             complement(8183..8719)
                     /locus_tag="WIC03_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ3882863.1"
                     /translation="MQAISPELQARAAKIKLLILDVDGVLTDGRIFIRDNGEEIKSFH
                     TLDGHGLKMLQASGVQTAIITGRDAPSVGIRVKQLGINYYFKGISDKRAAYEELRAQV
                     GVEEAECAFVGDDVVDLPVMVRCGLPVAVPDAHWFTLQHAAYIAKRPGGAGAVREVCD
                     LIMQAQGTLGAALNEYIK"
     gene            complement(8784..9758)
                     /locus_tag="WIC03_000303"
     CDS             complement(8784..9758)
                     /locus_tag="WIC03_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEZ3882864.1"
                     /translation="MAENGKYLDWAREVLHTEAEGLREIAAELDENFVLAADALLHCK
                     GRVVITGMGKSGHIGRKMAATMASTGTPAFFVHPAEAAHGDLGMIVDNDVVVAISNSG
                     ESDEIAAIIPALKRKDITLVCITARPDSTMARHADIHITASVSKEACPLGLAPTTSTT
                     AVMALGDALAVVLLRARAFTPDDFALSHPAGSLGKRLLLRVADIMHKGGGLPAVRLGT
                     PLKEAIVSMSEKGLGMLAVTDGQGRLKGVFTDGDLRRLFQECDNFTGLSIDEVMHTHP
                     KTIAAERLATEALKVMQANHVNGLLVTDADGVLIGALNMHDLLAARIV"
     gene            9855..10910
                     /gene="tal"
                     /locus_tag="WIC03_000304"
     CDS             9855..10910
                     /gene="tal"
                     /locus_tag="WIC03_000304"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEZ3882865.1"
                     /translation="MTILSDVKALGQQIWLDNLSRSLVQSGELAQMLKQGVCGVTSNP
                     AIFQKAFAGDALYADEVAALKQQDLTPKQRYETMAVADVQAACDVCLAEHESTGGKTG
                     FVSLEVSPELSKDAQGTVEEARRLYAAIGCKNAMIKVPATDAGIDALETLVSDGISVN
                     LTLLFSRVQTLKAYAAYARGIAKRLAAGQSVAHIHVVASFFISRVDGALDTTLPDHLK
                     GKIAIALAKAAYQDWEQYFTTPEFAALEAQGANRVQLLWASTGVKNPAYPDTLYVDSL
                     IGAHTVNTVPDATLKAFIDHGTAKATLTEGADEAQAQLAETAALGIDVETLAARLQED
                     GLKQFEEAFEKLLAPLV"
     gene            11064..11354
                     /locus_tag="WIC03_000305"
     CDS             11064..11354
                     /locus_tag="WIC03_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3882866.1"
                     /translation="MAKALEIISPNEIYSDLIFKDPVPPHTIYTELMKLVSRNASNER
                     IADRAFDFFSSGLMDGSATEEQYNALYDLTLLEEPGMELNKDEIMALINSLK"
     gene            11371..12258
                     /gene="gluQRS"
                     /locus_tag="WIC03_000306"
     CDS             11371..12258
                     /gene="gluQRS"
                     /locus_tag="WIC03_000306"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HEZ3882867.1"
                     /translation="MYTGRFAPSPTGLLHIGSLLTAAASYADARSNGGKWLVRMEDLD
                     PPREMPGAASHILHTLEAFGFEWDGEVAYQSRRYALYEETLCRLQTAGLVYPCHCSRK
                     DWQAAAVHGADGFVYNGRCRNPQQRPALQGKQPAWRIRVPDRDIGFSDGIVGGYAQNL
                     ARDIGDFVLLRADGYWAYQLAVVADDAEQGVTHIVRGQDLLVSTPRQIYLQQCLGVPT
                     PQYAHLPLLTNAQGQKWSKQTLAPALDLNRREQLLRQVFRYLKLPEAPETDRPAELLD
                     WAVAHWDMGKVPKHAITTP"
     gene            complement(12328..13338)
                     /gene="dusA"
                     /locus_tag="WIC03_000307"
     CDS             complement(12328..13338)
                     /gene="dusA"
                     /locus_tag="WIC03_000307"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HEZ3882868.1"
                     /translation="MNDNTHTLPPRHLSVAPMLDWTDRHYRYMARQITRNAWLYSEMV
                     NAGAIVYGDKDRFLMFNEGEQPVALQLGGSDPSDLAKAAKAAEAYGYNEVNLNCGCPS
                     PRVQKGSFGACLMNEVGLVADCLNAMQDAVGITVTVKHRIGVDRQTEYQTVADFVGTL
                     RDKTACKTFIVHARNAWLDGLSPKENRDVPPLKYDYVYRLKQEFPGLEIIINGGITTN
                     EAIAGHLQHVDGVMVGREAYHNPMVMREWDRLFYGDTRSPIEYADLVQCLYTYSQAQI
                     QAGRGTILRHIVRHSLGLMHGLKGARTWRRMLSDATLLKDNDGSLILEAWKEVERANT
                     RE"
     gene            complement(13369..13860)
                     /locus_tag="WIC03_000308"
     CDS             complement(13369..13860)
                     /locus_tag="WIC03_000308"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEZ3882869.1"
                     /translation="MRAVIQKTVGAKVDVVSENGTETCGKIDGGFVVLLGVTHDDTEK
                     DARYIADKIVHLRVFEDEAGKLNLSLKDVGGAVLLVSQFTLYADAASGRRPSFSQAAP
                     AEQAQQLYLRTAELLRGHGIHVETGRFRTHMQVSLCNDGPVTILLDSFMTRISPKMKV
                     VPD"
     gene            complement(13934..14692)
                     /locus_tag="WIC03_000309"
     CDS             complement(13934..14692)
                     /locus_tag="WIC03_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ3882870.1"
                     /translation="MKQKKTAATVIAAMLAGFAAAKAPEIDPALVDTLVAQIMQQADR
                     HAEQSQKPDGQAIRNDAVRRLQTLEVLKNRALKEGLDKDKDVQNRFKIAEASFYAEEY
                     VRFLERSETVSESALRQFYERQIRMIKLQQVSFATEEEARQAQQLLLKGLSFEGLMKR
                     YPNDEQAFDGFIMAQQLPEPLASQFAAMNRGDVTRDPVKLGERYYLFKLSEVGKNPDA
                     QPFELVRNQLEQGLRQEKARLKIDAILEENGVKP"
     gene            complement(14763..15629)
                     /locus_tag="WIC03_000310"
     CDS             complement(14763..15629)
                     /locus_tag="WIC03_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ3882871.1"
                     /translation="MKAKILTSVALLACSGSLFAQTLATVNGQKIDSSVIDAQVAAFR
                     AENSRAEDTPQLRQSLLENEVVNTVVAQEVKRLKLDRSAEFKNALAKLRAEAKKSGDD
                     KKPSFKTVWQAVKYGLNGEAYALHIAKTQPVSEQEVKAAYDNISGFYKGTQEVQLGEI
                     LTDKEENAKKAVADLKAKKGFDAVLKQYSLNDRTKQTGAPVGYVPLKDLEQGVPPLYR
                     AIKDLKKGEFTATPLKNGDFYGVYYVNDSREVKVPSFDEMKGQIAGNLQAERIDRAVG
                     ALLGKANIKPAK"
     gene            complement(15678..15956)
                     /locus_tag="WIC03_000311"
     CDS             complement(15678..15956)
                     /locus_tag="WIC03_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HEZ3882872.1"
                     /translation="MSAVDLIRERLQTLDPLVLEIGDESHLHKGHAGNTGGGHYAVLV
                     VSGRFEGVSRLNRQKTVKSLLKDLFSGGMIHALGIRAATPDEYFHTAD"
     gene            complement(15956..16246)
                     /locus_tag="WIC03_000312"
     CDS             complement(15956..16246)
                     /locus_tag="WIC03_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEZ3882873.1"
                     /translation="MEYFMLLATDGENVYEARMAARPEHLKRLETLKSEGRLLTAGPN
                     PLPEDSNRVSGSLIVAQFESLDAAQAWAEDDPYVHAGVYSEVLIKPFKAVFK"
     gene            complement(16249..16779)
                     /locus_tag="WIC03_000313"
     CDS             complement(16249..16779)
                     /locus_tag="WIC03_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HEZ3882874.1"
                     /translation="MKFVSDLLSVILFFATYTVTKNMIAATAVALVAGVVQAAFLYWK
                     YKKLDTMQWVGLVLIVVFGGATIVLGDSRFIMWEPSVLFWLGALFLWGSHLAGKNGLK
                     ASIGREIQLPDAVWAKLTYMWVGFLIFMGIANWFVFTRFESQWVNYKMFGSTALMLVF
                     FIIQGIYLSTCLKKED"
     gene            16985..>19123
                     /locus_tag="WIC03_000314"
     CDS             16985..>19123
                     /locus_tag="WIC03_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV family protein"
                     /protein_id="HEZ3882875.1"
                     /translation="MPAGRLPRRCPMMTKFTDCTRSNRIQPPTHRGYILKNNRQIKLI
                     AASVAVAASFQAHAGLGGLNIQSNLDEPFSGSITVTGEEAKALLGGGSVTVSEKGLTA
                     KVHKLGDKAVIAVSSEQAVRDPVLVFRIGAGAQVREYTAILDPVGYSPKTKSALSDGK
                     THRKTAPTAESQENQNAKALRKTDKKDSANAAVKPAYNGKTHTVRKGETVKQIAAAIR
                     PKHLTLEQVADALLKANPNVSAHGRLRAGSVLHIPNLNRIKAEQPKPQTAKPKAETAS
                     MPSEPSKQATVEKPVEKPEAKVAAPEAKAEKPAVRPEPVPAANTAASETAAESAPQEA
                     AASAIDTPTDETGNAVSEPVEQVSAEEETESGLFDGLFGGSYTLLLAGGGAALIALLL
                     LLRLAQSKRARRTEESVPEEEPDLDDAADDGIEITFAEVETPATPEPAPKNDVNDTLA
                     LDGESEEELSAKQTFDVETDTPSNRIDLDFDSLAAAQNGILSGALTQDEETQKRADAD
                     WNAIESTDSVYEPETFNPYNPVEIVIDTPEPESVAQTAENKPETVDTDFSDNLPSNNH
                     IGTEETASAKPASPSGLAGFLKASSPETILEKTVAEVQTPEELHDFLKVYETDAVAET
                     APETPDFNAAADDLSALLQPAEAPSVEENITETVAETPDFNATADDLSALLQPSKVPA
                     VEENAAETVADDLSALLQPAEAPAVEENVTE"
BASE COUNT         4547 a         5407 c         4822 g         4347 t
ORIGIN      
        1 tcatattcag gataggcaga tggcattttt ttaaccccgt aagtaagcaa tctttctccc
       61 atagtcgtac ttaaactaca cgcatctttt cggataacaa tatcgtccac gctatccaaa
      121 ccgtcgcgta gaaattggat aaagtgcgct ttgttctgct ggttgtatgg ttcgagcacc
      181 cagccttggt cggttttttt gtaatagacg ctaatctcta tcaaattaat ggagtaagca
      241 tcctgcccgg gcatgtcgga aagccatttt gtggcattgt cttgcgtata ccgtgcaaga
      301 tagccatagg ggataaccat tttggtgccc tgaaaatcaa atgtaaccgt atgttcaaat
      361 cctgtcattg gctcgggatt gttgaaactg gtttgttcat taacaggttc acatgaggtc
      421 atagtaaaaa cactcccaat aaacaaaata aaaagttgat aaatggtagt agcatatggt
      481 ctcataattt caagcttaga aattagttaa agaatatatt catggtagat actaactctt
      541 taagttgaca tattgtgcga tgtcgagggc tgtaccgtat tttcccatcg tattttcccg
      601 ttctttatat gagcggcagg catcctcata aaagaaggga taccgataag gaaaaatacc
      661 ccggcataaa aaatatactg attaatcaat acaaatccaa cagacagttc atcgtattct
      721 tttctgttgg cgtataaaaa atactgatat atgccttcac tttcataggg cgacgcttta
      781 tgtaccgtcc tgtgtgttga aacgtcggtt ttccttaccg cctgccccgc ttttccctga
      841 ttgctgccaa ctgccgtccg aacctgaacc gtcaggtttc agacggcatt ttttctgcct
      901 gctcccacaa ttgccgtttt tgtgcggcgg tcagtttttt gacggcgatt tcctgcctga
      961 gtgccgttcc tttatccatc ccgcctgcaa cgatacgcat cgccaccggt ttgaatacgc
     1021 gggtatattt cgcgccttta ccggttgtgt gggcggcaag ccgctgttgc ggattcgggc
     1081 tgatgccgca atagaacgcg ctgttttcac acaatatcag gtaaacgctc cagtttgacg
     1141 cgttcatctg cctgcatcct gagttttctc cccgttccaa ggcgcaactt tcagcagcag
     1201 cagcataccc ggcagggcaa gtatgaaaca cagctggaaa aacggtacat acccgagcca
     1261 ttcaatcaga taaccggcaa aggaattgat gaccgtacgg ggaacagcgg acaggctggt
     1321 aaacagtgca agctgcgttg cggtaaatgc gggattggtc tcgcgcgcca tatacgatac
     1381 aaacgccgcc gtccccaaac ccacgccgac tgcctctgcg ccgataactg ccgccagcat
     1441 cagcctctcg cctgtgccga ccgtgtcgaa atgtccgaac cctgccagcc atacaaaccc
     1501 caaaacggtt acagcctgca ccgcgccaaa tatccacaag gctttgttta cgccgatttt
     1561 cagcatccac acgccgccca agatacctgc cgccactgcc ggccacagtc ctgcattttt
     1621 tgcaatcaaa ccgatgtctg tcttgctgaa acccatatcc agataaaacg gcgttgccaa
     1681 cgcggttgcc atactgtcgc cgagtttgta aaggaagata aacagcagca cgcacaccgc
     1741 cgaagcgatg cccttgcgcg taaaaaattc tttaaacggc tctaccacgg tctgcttcaa
     1801 cgttttagga acggcaggcg gcaacacggg ttcgcgcgcg agaaacagcg tcatcagaag
     1861 gccgggcagc ataaataatg aagtgataac aaatacttct gaccacggca tcctgtctgc
     1921 caacaccaaa ctcaatgaac cgggaatcag ggcggcaatc cggtaggcgt tcacatgaac
     1981 cgagttgccc aaacccaatt cttcgtctga caaaatctcg cgcctgaacg catccaatac
     2041 aatatcctga ctggcggaaa aaaaagcgac aagcaccgac aagccggcaa tcagcggcag
     2101 atgattacgg gggtttaaaa aggcataggc cgccaaagcc gccagcaacc ctgcctgcgt
     2161 cagcagcatc cacccgcgcc gccgtcccaa aacgggcagc ctgaccgcgt ccatcagcgg
     2221 cgaccacaaa aatttccaag taaacggcag accgattaac gccatcagcc cgatgctctt
     2281 caaatccacc tgctcgctgc gcaaccacgc cggaatcagg ttaatcagaa agtacagcgg
     2341 cagccccgag gtaaaccccg taaaaataca gacaagcata tttcgggaaa agatttgccc
     2401 gataaaacct gatttcgatg cagtcatcgt tcgtgcagcc caaaaaaaac aaaagcctat
     2461 tgtagcaaat atcgcccgcc tgctgaaaaa tgccgccccg cgccgccggt ttgccgttca
     2521 aatgcaaaat gccgtctgaa actgtgttca gacggcattg cttttctgaa aaccagacgt
     2581 gcttacaggc tgtaatacat ttcaaattcc agcgggtgcg gcgccatgcg gatgcggcgt
     2641 acgtcttctt ctttgaaagc gatgtagctg tcgatccagt ctttgctgaa cacgccgccg
     2701 cgcaggagga attcgtggtc ggctttgagg gcggccagtg cttcttccaa agaagcgcaa
     2761 acggtcggca ccaatgcatc ttcttccggc ggcagatcgt acaggttttt atcggcaggg
     2821 tcgcccggat ggattttgtt ttgaatgccg tccaaacccg ccatcaacag ggcggcaaat
     2881 gccaaatacg ggttggcggt cggatcgggg aaacgcgctt cgatgcggcg cgccttgctg
     2941 ctgttcacgg acggaatgcg gatggaagcg gaacggtttt tggcggagta tgccagtttg
     3001 gtcggcgctt caaagtgcgg cacgaggcgt ttgtaggagt tggtggacgg attggtaatc
     3061 gcgttcaagg ctttggcgtg tttgatgatg ccgccgatgt agtagagcgc ggtgtcgctc
     3121 aagccggcat agccgtcgcc tgcgaacagg ttttgaccgt ctttccaaat ggattggtga
     3181 acgtgcatac cgctgccgtt gtcgcccata atcggtttgg gcataaaggt ggctgttttg
     3241 ccgaagttgt gggcaacgtt ttgaatcaca tatttcatgt cttgggtttg gtcggcgcgt
     3301 ttgaccaagg tggcgaaacg cgtgccgatt tccatttggc tgccggtacc gacttcgctg
     3361 tggtggactt cgacttcgat gccgagttct tccaaaatgt tcaccatggc ggagcgcaaa
     3421 tcttggccgc aatcaatcgg cgcgacgggc gcgtaaccgc ctttaacggc ggggcggtgg
     3481 ccggtgtttt gaccgtccat atgcagaccg cttgcccatg cgccgctttc ggacgtgatt
     3541 tcgtaacggg ttttgtgcat atcggtttca aattctacgc cgtcgaagac gaaaaactcg
     3601 ggttcggggc cgaagtatgc cgtgtcgccg ataccggaag atttcaaata ggcttcggcg
     3661 cggcgtgcga tggagcgcgg gtcgcggtcg taaccctgac cgtcggcagg gtcgatgacg
     3721 tcgcaggtaa tgacgacggt aacatcatca taaaaaggat caacgaaggc tgtagacgca
     3781 tcgggacgca gctgcatatc ggaagcctga atgcctttcc agccgccgat agacgaaccg
     3841 tcaaatgcct gaccgttttc aaaccactct tcagggtctt ccaacacgat gcgtgcaggc
     3901 acggtaaagt ggtgctgctt gcctttggta tcggtaaagc gcaaatcgac aaagcgggct
     3961 tcgctttctt caatcaattt tacgacgttt ttaatagaca tttttcagct cctggaaagg
     4021 ggaaatagca aaatatgcgg aacgcggcgg cttgacgcaa agtcggcacg attctaccac
     4081 gtccatgccg tctgaaactc gccctgcgtc aaattccaag ctgtaaaata tagtttcaga
     4141 cgacaagatt gtcaacaata tggataaaat tgcatatcat gtccggattt ctccccagct
     4201 tatacaggga aaaaccgtac cgcctgacaa ccgtttcaga cggcatcgtt tccatctgta
     4261 cgccgccgtt ataaaatagc cttcccgtct tttggaacac gaccatgacc gccctccccc
     4321 gttactccgt tttcggcaac cccgttgcac acagtaaatc tccgcagatt catcggcaat
     4381 ttgcccttca ggaaggcgtt gacatcgaat acggacgcat ttgcgccgac atcggcggtt
     4441 tcgctcaggc ggtttcgaca ttttttgaaa caggcggttg cggggcaaac gttaccgtac
     4501 cgttcaaaca ggaagcgttt catctggcgg acgaacattc cgaccgcgct ttggcagcag
     4561 gtgcggtcaa tacgctgatt ctgttgaaaa acggcaagct gcgcggcgac aatactgacg
     4621 gtatcggttt ggccaacgac atcacgcagg ttaaaaacat tgccatcgag ggcaaaacta
     4681 ttttactttt gggcgcgggc ggtgcggtgc gcggcgtgat tcctgttttg aaagaacacc
     4741 gtcctgcccg tatcgtcatt gccaaccgta cccatgccaa agccgaggaa ttggcgcagc
     4801 ttttcggcat tgaagccgtc ccgatggcgg atgtgaacgg cggttttgat atcatcatca
     4861 acggcacatc cggcggtttg agcggtcagc ttccggccgt caatcctgaa attttccgcg
     4921 actgccgcct tgcctacgat atggtgtacg gcgaagcggc aaaaccgttt ttggattttg
     4981 cccggcaatc gggcgcgaaa caaaccgccg acggactggg tatgctggtc ggtcaggcgg
     5041 cggcttccta cgccctctgg cgcggattta cgcccaatat ccgccctgtt atcgaataca
     5101 tgaaagccat gtaatatgtt ccgcatcgtc aaatggctga ttgccctgcc cgtcggcatc
     5161 tttatctttt tcaatgccta tgtgtacggc aacatcatca cctaccgcgc cgtcgcgccg
     5221 catcggactg cctttatgtc gatgcggatg aagcagtttg aacaggaagg tcgcgatgtc
     5281 gcactggatt accgctgggt accctacaac cgcatctcgg tcaacctgaa aaaagccctg
     5341 attgcttccg aagatgcccg tttcgccgga cacggcggct tcgattgggg cggcattcaa
     5401 aacgccatca ggcgcaaccg gaacagcggc aaagtgaagg cgggcggttc gaccatcagc
     5461 cagcagcttg ccaaaaacct gtttttaaac gaaagccgca gctatatccg caaaggcgaa
     5521 gaagcggcga ttaccgcgat gatggaagct gttaccgaca aagacaggat ttttgaactg
     5581 tatttaaact caatcgagtg gcactacggt attttcggcg cggaagccgc gtcccggtat
     5641 ttttatcaaa tacccgccgc caaactgagc aaacagcagg cggcgaaact gacggcgcgc
     5701 gtccccgccc cgctctacta cgccgaccat ccgaaaagca aacggctgcg caacaaaacc
     5761 aatatcgtgc tcagacgcat gggttcggca gaattgcctg aaagcgatac ggactgagtg
     5821 ccgccgcaat ataaaatgcc gtctgaaata tcattcagac ggcatatgct ttctgccttt
     5881 accctcttct ccaaaacgca acggggcggt cattatcatt tttgcccttc tacataacgg
     5941 tcagacacaa aacgtgtctg acacggacaa cttcccaaaa acggcaggca aaaaaaataa
     6001 ccgcttcaag gcggtttttt ggtggccaga ggcggaatcg aaccgccgac acacggattt
     6061 tcaatccgtt gctctaccaa ctgagctatc tggcctttgt ctgtctcgtt aagagatgcg
     6121 tattaaacca aattacggga cgacatgcaa gcatatcggg caatattctt tgaaaacagg
     6181 tttaagtcaa tgttttaaat ttgaattaat ttatgtaaaa acaacataaa tccgtatttt
     6241 tgccgcctgc ccgacgagat taggccaccc gtctgaaaaa tattttcaat atttgaagtt
     6301 ctcacccaga taaacagaac gaacctgttc gtttccgacc aaatcatcag gttttcccga
     6361 tgccaacacc gtgccgtctg aaataatgta ggcccgatcg cagatgctga gggtttcgcg
     6421 tacgttgtgg tcggtaatca atacgccgat accgcgcgac ttgagaaatc cgatgatttt
     6481 ctggatgtcg atgacggcga tgggatcgac gccggcaaaa ggctcgtcca acaaaataaa
     6541 atgcggtttc atggcgagta cgcgggcgat ttcgacgcgt cggcgttcgc cgcctgacaa
     6601 agatggggca ggattattgc gcaaacgctc gatattcagg tcggcaagca gtttttcgat
     6661 ttccctgtcg atttgatttt tatctttggt tctgatttcc aagatggcgc ggatgttttg
     6721 ttcgacggtc attttacgga aaatagaggc ttcctgcggt aggtagccga cacccaggcg
     6781 ggcgcgttcg tgtatgggca ggtgacgcaa ttcctgcccg tccagcatca cgctgcccgc
     6841 atcggcggca atgagtccga ctatcatgta gaagctggtg gttttacccg caccgttggg
     6901 cccgagcagt ccgatgactt cgccgctttc gatttcgagg gagaagcttt taacgacttg
     6961 gcgtttttta aaactttttt gcaggttttg aacaacaagg cggctgacgt ttgcgctcat
     7021 aaatatctct tcaatatctt cggtcggcat atgccgtccg aaccgggttt atgtttcaga
     7081 cggcattttg ggattattcg gatttttgcg tactcgaagg ctggataacg acgctgaccc
     7141 tgccggattt ggaagcggat tttgcgccgg attttgtgct gccgctgatg gtatagactt
     7201 cggttttggt gttgtatgta atcaccgcac cttcggcgac atcgccgccg cgctgtactt
     7261 tggcattacc ggttaagact acggtgctgc ctgcagatga ataagcaacg ttgtttgcct
     7321 gtccgcgcac cgtgcctttg ccgccgtcca atgtctggct gaagcggact ggcgaacctt
     7381 ccgccctcac ggattcgccg cctttgccgc cgcgtgtaac attgacgcgg gcggcggaaa
     7441 tattgagcgt accctgtctg atgacgacgt ttccgctgaa tgtggtgctt tggttggctt
     7501 gatcgagcga accttggtcg gcctcaatct gaataggctg cctgctgtcg ctttgaaggg
     7561 caaaagcggg ggacgccgaa aaaaatgcta ttaaaacaaa tagcttacat atcttttgta
     7621 tcataaatcg tggctttcac tttagatgag aagttcaaca ggcctgtttt gtggtcgtaa
     7681 gtcatgccgc ccgcctgccc gtgcgacgcg ccgtattgaa agctgacagg cgtatcggtt
     7741 tgggcatatt gagattcggt atcgacgtgc agcttttcgg ttttaacttt acccgcctgc
     7801 cgtttgccat cggcggtttt ggtcagcaca acgttgtttt taaaaagaac ctgtttgttt
     7861 tcggtatggt aaacggcttc atcgctgccg acttcgtaca acaacctgcc ttcttggaag
     7921 aacacgagat gcggtgaatc gaaatgaatg tcgctgcttt cgggaaactg tttcgcgcct
     7981 ttcgcgctca aatgttcttt taagtatccc tgttcgtcaa accgcctgcc atccaagccg
     8041 tccattgtgt attgcggttc gtcgggattg agcctgactt cttcaatctc gacttcgctg
     8101 atgcgtccca accatgccga cagaccgccc aaggcaaccg ccaatatcaa tgggaacgca
     8161 attccgtacc gccatcttat tttcatttga tgtactcgtt caaagccgcg cccaaagtcc
     8221 cttgcgcctg cataatcagg tcgcacactt cgcgcaccgc gcccgcaccg ccggggcgtt
     8281 tggcaatata ggcggcgtgt tgcagcgtaa accaatgcgc gtcggggacg gcaaccggca
     8341 atccgcaacg caccatcacc ggcaaatcga ccacgtcgtc gccgacaaag gcacactcgg
     8401 cttcttccac gccgacctga gcgcgcagct cttcatacgc ggcgcgtttg tccgaaatgc
     8461 ctttgaagta gtaatttatg cccaactgtt tgacgcggat gccgacggag ggcgcgtccc
     8521 gacctgtgat aatcgcagtc tgcacgccgc ttgcctgaag cattttcaga ccgtgtccgt
     8581 ccagtgtgtg aaacgatttg atttcttcgc cgttatcgcg gataaagatg cgcccgtcgg
     8641 tcaaaacgcc gtccacatcc aggatcagca gtttgatttt ggcggcgcgc gcctgtaatt
     8701 caggagaaat tgcctgcatg ggatttcctt gttttcagac ggcatatctt accacaaata
     8761 ccgtctgaaa cggctgcaac gccttataca atccgcgccg ccaaaaggtc gtgcatattc
     8821 agcgcgccga tcagcacgcc atctgcatcg gtaaccagaa gcccgttcac atggtttgcc
     8881 tgcatgactt tcagggcttc ggtggcgagg cgttcggcgg cgatggtttt agggtgtgta
     8941 tgcatgactt cgtctatcga aagaccggta aaattgtcgc attcttgaaa caggcggcgc
     9001 aaatcgccgt cggtgaatac gcctttcaga cggccttgcc cgtccgttac cgccagcatc
     9061 cccagccctt tttcactcat gctgacgatg gcttctttca agggcgtgcc gagtcggacg
     9121 gcaggcaggc cgccgccttt gtgcataatg tcggcaacgc gcaaaagcag gcgtttgccg
     9181 aggctgccgg cagggtggct caaggcgaag tcgtcgggcg tgaacgcgcg ggcgcgcagc
     9241 aggacaaccg ccaacgcatc gcccaaagcc ataacggcgg tggtgctggt ggtcggggca
     9301 agccccagcg ggcaggcttc tttggaaacc gacgccgtga tgtggatgtc ggcatggcgc
     9361 gccatggttg aatcggggcg ggcggtgatg cagacgaggg taatgtcttt gcgtttgagt
     9421 gcggggatga tggcggcgat ttcgtcgctt tcgccggaat tggaaatcgc gacgacgacg
     9481 tcgttgtcca caatcatgcc caagtcgccg tgtgccgctt ccgcagggtg gacgaaaaac
     9541 gcgggcgtgc cggtcgaggc catggttgcc gccattttgc gcccgatatg tcccgacttg
     9601 cccatgcccg tgataacaac cctgcccttg cagtgcaaca acgcatctgc cgcaaggacg
     9661 aagttttcgt ccaattccgc tgcaatttcg cgcaagcctt ccgcttcggt gtgcaacact
     9721 tcgcgcgccc agtcgagata ttttccgttt tctgccattg ccatccgcct ttgctgtaat
     9781 aaaactaccc aaaatttccg aatcggggta gcataagcct ttgaaacata cacatccatc
     9841 caaaggaatc agaaatgact attttatcgg acgttaaagc attaggacaa caaatctggc
     9901 tggacaacct ttcccgctcg ctcgtgcaaa gcggcgaatt ggcgcaaatg ttgaaacaag
     9961 gcgtgtgcgg cgtaacttcc aatcccgcca ttttccaaaa agccttcgcc ggcgatgccc
    10021 tgtacgccga tgaagtcgcc gcgctcaaac agcaagatct gaccccgaaa caacgctacg
    10081 aaaccatggc ggttgccgat gtgcaagccg cctgtgacgt gtgccttgcc gaacacgaat
    10141 ccaccggcgg caaaaccggt ttcgtcagcc tcgaagtttc gcccgaattg tccaaagacg
    10201 cgcaaggcac ggtagaagaa gcgcgccgcc tctacgccgc catcgggtgc aaaaacgcga
    10261 tgattaaagt gcctgccacc gacgcaggca tcgatgcgct cgaaaccctc gtttcagacg
    10321 gcatcagcgt aaacctgacc ctgctgttct cacgcgtcca aaccctcaaa gcctacgccg
    10381 cctacgcgcg cggcattgcc aaacgcttgg cagccggaca aagcgttgcc catatccacg
    10441 tcgtcgccag cttcttcatc tcccgtgtgg acggcgcgct ggacacaacg ctgcccgacc
    10501 acctcaaagg caaaatcgcc atcgcccttg ccaaagccgc ctatcaagat tgggaacaat
    10561 atttcaccac ccccgaattt gccgcactgg aagcccaagg cgcaaaccgc gtgcagcttt
    10621 tatgggcatc taccggcgtg aaaaacccag cctatcccga cacgctctac gttgacagcc
    10681 tgatcggcgc gcacaccgtc aacaccgttc ccgatgccac gctcaaagcc ttcatcgacc
    10741 acggcacagc caaagctacg ctgaccgaag gcgcggacga agcacaagct cagctcgccg
    10801 aaaccgccgc gctcggcatc gatgtcgaaa ccttggcggc acgtttgcag gaagacggtt
    10861 tgaaacagtt tgaagaagcg tttgaaaaac tgctcgcacc gttggtttaa acttttcatc
    10921 cccgatgccg tctgaaaggc tttcagacgg catcgcccgt ttgaccggac acagtgcggc
    10981 atatcaagga ttcaccctaa aaatattaat gggcaagaag ttaatgcacg acattaccca
    11041 ccaacacaaa aggaaataga atcatggcaa aagcactcga aatcatttca cccaatgaaa
    11101 tttattcaga tctgattttt aaggatccgg tacctcccca tacaatatat acagaattga
    11161 tgaaactggt tagtagaaat gcaagtaatg agagaatcgc agatagagcc ttcgattttt
    11221 tctcgtccgg ccttatggat ggttcagcta ccgaagagca atataatgcc ctatatgatt
    11281 taaccttact tgaagaaccc gggatggaac tgaataaaga tgagataatg gctttgatta
    11341 atagtcttaa ataaaggggc cgtaccggat atgtacacag gacgtttcgc ccccagcccg
    11401 accgggctgc tccacatcgg ctcgctgctg accgccgccg cttcctatgc cgatgcacgc
    11461 tcaaacggcg gcaaatggct ggtccgcatg gaagacctcg atccgccgcg cgaaatgccg
    11521 ggggcggcaa gccatatcct gcacacgctt gaggcattcg gattcgagtg ggacggagaa
    11581 gtcgcctatc agagccgccg ttacgccctg tatgaagaaa cactatgccg tctgcaaacc
    11641 gccggactgg tctatccctg ccattgcagc cgcaaagact ggcaggctgc ggcagtccac
    11701 ggcgcagacg gattcgtcta taacggacgt tgccgcaatc cgcagcaacg ccctgcactg
    11761 caaggcaaac agcctgcgtg gcgcatccgc gtccccgacc gcgacatcgg cttttcagac
    11821 ggcatcgtcg gcggttacgc ccaaaacctc gcccgcgaca tcggcgattt cgtcctgctt
    11881 cgtgcagacg gttactgggc ataccagctc gccgtcgttg ccgacgatgc cgaacagggc
    11941 gttacccaca tcgtccgcgg acaagacctg ctcgtttcca cgccgcgcca aatctatttg
    12001 cagcagtgtt tgggcgttcc gacaccgcaa tatgcccacc tgccgctgct gaccaacgca
    12061 caagggcaga agtggtcgaa acagacgctc gcccccgcat tggatttaaa ccgccgcgaa
    12121 caactcctcc gccaagtgtt ccgttacctc aagctgcccg aagcaccgga aaccgaccgc
    12181 cctgccgaac tgctcgactg ggcggtggca cactgggata tgggcaaagt gccgaaacac
    12241 gccattacca ccccctaaac accgcccgac aaacgcacaa atgccgtctg aagccttcag
    12301 acggcatttc acacatacag ccccgcccta ttcgcgcgta tttgcccgct caacctcttt
    12361 ccacgcttcg agaatcaggc tgccgtcgtt gtctttcaag agcgttgcgt cggaaagcat
    12421 acgccgccaa gtccgcgcac ctttcaaacc gtgcatcagc ccgaggctgt ggcggacgat
    12481 gtggcgcaag attgtgccgc gtccggcttg gatctgggct tggctgtatg tgtagaggca
    12541 ctgcaccaaa tcggcgtatt cgatcgggct gcgagtatcg ccgtaaaaca atctgtccca
    12601 ttcgcgcatc accatcgggt tgtggtacgc ctcgcgcccg accatcacgc cgtcaacgtg
    12661 ttgcaggtgt ccggcgattg cttcgttggt ggtgatgccg ccgttgatga tgatttccag
    12721 cccgggaaac tcctgcttga ggcggtaaac ataatcgtat ttcaagggcg gaacgtcgcg
    12781 gttttctttg ggtgaaagac cgtccagcca agcgttgcgg gcgtggacga taaaggtttt
    12841 gcaggcggtt ttgtcgcgca gcgtgccgac gaaatcggca acggtttggt attcggtctg
    12901 cctgtccaca ccgatgcgat gtttgacggt aacggtaatg ccgaccgcgt cctgcatggc
    12961 gttgaggcag tcggcaacca gcccgacttc gttcatcaga cacgcgccga acgaaccttt
    13021 ctggacgcgc ggactgggac agccgcagtt gaggttgacc tcgttgtagc cgtatgcttc
    13081 ggcggctttg gcggctttcg ccaaatcgga cggatcgctg ccgcccagtt gcagggcgac
    13141 gggctgctcg ccttcattga acatcaaaaa gcggtctttg tcgccgtaaa caatcgcgcc
    13201 ggcgttgacc atttcgctgt acaaccaagc atttcgggta atctggcgcg ccatataacg
    13261 gtagtgcctg tccgtccagt cgagcatggg ggcgacggac aggtggcgcg ggggaagggt
    13321 gtgtgtattg tcgttcattg tcgatatttc accattgcgg attcaatttc aatccggaac
    13381 aaccttcatt tttggggaaa tccgcgtcat gaaagagtcc agcagtatgg ttaccggccc
    13441 atcgttgcag agcgacacct gcatatgcgt gcggaaacgc cctgtttcga catgaatccc
    13501 gtgtccgcgc aacagttccg ccgttcgcag gtaaagctgc tgcgcctgtt ctgcaggtgc
    13561 ggcttgggaa aacgaaggcc gccgcccgct tgccgcatcg gcataaagtg taaactgcga
    13621 caccagcagc accgcgccgc cgacatcttt caaagacagg ttcaacttgc ccgcttcgtc
    13681 ttcaaacacg cgcaaatgga cgattttgtc ggcgatatag cgcgcatctt tttctgtgtc
    13741 gtcgtgcgtt acgccgagca gcacgacgaa tccgccgtcg attttgccac aggtttccgt
    13801 gccgttttcg gacacgacat ccacctttgc acctaccgtt ttctgtatga ccgcacgcat
    13861 aagccgtcct ccttacctga acgtgcaatg ccgtctgaaa aggcttcaga cggcatcggt
    13921 attggaaatg ccattacggt ttgacaccgt tttcttccaa aatggcatcg attttcaagc
    13981 gggctttttc ctgtctcaaa ccttgttcca actggtttct gaccaactcg aaaggctgcg
    14041 cgtcggggtt tttcccgacc tcgctgagtt tgaacagata atagcgttcg cccaatttga
    14101 ccggatcgcg ggtaacgtcg ccccgattca tcgcggcaaa ctgcgaagcc agcggctcgg
    14161 gaagctgctg cgccataatg aaaccgtcaa aagcctgctc gtcgttcgga taacgcttca
    14221 tcagcccttc aaaagacagc cctttgagca ggagctgctg cgcctgacgc gcctcctctt
    14281 cggttgcgaa gctgacctgc tgcaatttga tcatgcggat ttgccgctca taaaactgac
    14341 gcagtgcgct ttcggaaacc gtttccgaac gttccagaaa acggacgtac tcctcggcat
    14401 aaaaagacgc ttcggcgatt ttaaagcggt tttggacatc cttatcctta tccaaacctt
    14461 ccttcaatgc cctgtttttc aaaacttcca aagtttgcag ccgacggacg gcatcgtttc
    14521 ggattgcctg cccgtccggt ttttgggact gctccgcatg ccggtctgcc tgctgcatga
    14581 tctgcgccac cagcgtatcc accaaagccg ggtcgatttc gggtgctttg gctgccgcaa
    14641 aacctgccaa cattgcagca ataactgtgg cagcggtttt tttctgtttc atggtattcc
    14701 ctgtcctgcg cggcggcaaa acgcctgtct gaacgtcttg ccgccatatc ccgttttcag
    14761 aattattttg caggtttgat gtttgccttg cccaacagtg caccgacggc acggtcaatc
    14821 cgttccgcct gaaggttgcc ggcaatctgt cctttcattt catcaaaaga aggcactttt
    14881 acctcgcggc tgtcgttgac ataataaacg ccgtagaaat cgccgttttt cagcggcgtt
    14941 gccgtaaatt cgcctttttt caagtcctta attgcccgat aaagcggcgg aacaccctgt
    15001 tccaaatctt tcagcggcac atatccgacc ggcgcgccgg tctgtttggt acggtcgttg
    15061 agggaatatt gtttcaagac ggcatcgaaa ccttttttcg ccttcaagtc ggcaaccgct
    15121 ttttttgcat tttcttcctt gtcggtcagg atttcgccca actggacttc ctgcgtacct
    15181 ttgtaaaaac cgctgatatt gtcatatgcg gcttttactt cctgctcgga aaccggttgg
    15241 gttttggcga tatgcagtgc gtatgcctcg ccgttcaagc catattttac cgcctgccaa
    15301 acggttttga aggacggttt cttgtcgtcg cccgactttt tcgcttcggc acgcaatttg
    15361 gcaagcgcat ttttaaactc tgccgaccgg tcgagtttca ggcgtttcac ttcctgtgcg
    15421 accacggtgt tgaccacttc gttttccagc agggattggc gcagttgcgg cgtgtcttcg
    15481 gcacggctgt tttccgcacg gaatgcggca acctgcgcat cgatgacgga actgtcgatt
    15541 ttctgaccgt tgacggttgc cagcgtttgg gcaaacaggc tgccggaaca ggcaagcagt
    15601 gcaacggaag tcaggatttt tgctttcata atcttctctc tttaaatttt aaattgaaat
    15661 gttcgggcag acttcattca gtccgccgta tggaaatact cgtcaggggt agccgcccgg
    15721 atgccgagcg cgtgaatcat gccgcctgaa aacaaatctt tgagcagcga tttgaccgtt
    15781 ttctggcggt tcaggcggct tacgccttca aaacggccgc tgacgaccaa aacggcataa
    15841 tgtccgccgc cggtattgcc cgcgtgtcct ttgtgcagat ggctctcatc gccgatttcc
    15901 aacaccagcg gatcgagcgt ctgcaggcgt tcgcggatca aatcgacggc cgacattatt
    15961 tgaacaccgc tttaaacggc ttgatcagca cttcgctgta cacgcctgca tgaacatagg
    16021 gatcgtcttc cgcccaagcc tgcgccgcgt ccaaagactc gaactgcgcc acaatcaaac
    16081 tgcccgaaac gcggttggag tcctccggca aaggattcgg gcctgccgtc aacagccggc
    16141 cttccgactt cagcgtctcc agccgtttga ggtgttcggg acgtgccgcc atacgcgctt
    16201 catacacatt ctccccgtct gttgccagca acataaaata ttccacagtc aatcctcctt
    16261 tttcagacag gtactcagat aaataccctg aataataaag aaaacaagca tcagtgcagt
    16321 cgagccgaac attttatagt tgacccattg cgactcgaac cgggtaaaca caaaccagtt
    16381 ggcgataccc ataaaaatca ggaaaccgac ccacatatac gtcaatttcg cccatacggc
    16441 atccggaagc tgaatctccc tgccgatact cgccttcaag ccgtttttac cggcgaggtg
    16501 gctgccccac aggaacagcg cacccagcca aaacaaaacg ctcggctccc acataatgaa
    16561 gcggctgtcg cccaaaacaa tggttgcgcc gccgaatacg acaatcagca ccagtccgac
    16621 ccactgcatc gtatccagct ttttatattt ccaatacaga aaagccgcct gaaccacacc
    16681 ggcaaccaag gcaaccgccg tagcggcaat catatttttg gtaacggtat aggtggcgaa
    16741 aaacaggatg acggacaaaa ggtcgctgac gaatttcata tccaaatgag aaagcaaatc
    16801 aaataaacgc taatcataca ggcagaaccc tgtttttgca cctgcaaaac caacaaattt
    16861 tgttttgatt gatgcaaata tgcacagatg tttttgaaaa aagatggaga tatgtcataa
    16921 tttgtaaaaa cggcgaatga ttgccgttta aaatgtggcg gcggtcggta cattcacata
    16981 ttaaatgccc gccggccgac tgccccgccg atgcccgatg atgacgaaat ttacagactg
    17041 tacgcggtca aaccgtattc agccgccaac ccacagggga tacatcttga aaaacaacag
    17101 acaaatcaaa ctgattgccg cctccgtcgc agttgccgca tcctttcagg cacatgctgg
    17161 actgggcgga ctgaatatcc agtccaacct tgacgaaccc ttttccggca gcattaccgt
    17221 aaccggcgaa gaagccaaag ccctgctagg cggcggcagc gttaccgttt ccgaaaaagg
    17281 tctgaccgcc aaagtccaca agttgggcga caaagccgtc attgccgttt cttccgaaca
    17341 ggcagtccgc gatcccgtcc tggtattccg catcggcgca ggcgcacagg tacgcgaata
    17401 caccgccatc ctcgatcctg tcggctactc gcccaaaacc aaatctgcac tttcagacgg
    17461 caagacacac cgcaaaaccg ctccgacagc agagtcccaa gaaaatcaaa acgccaaagc
    17521 cctccgcaaa accgataaaa aagacagcgc gaacgcagcc gtcaaaccgg cgtacaacgg
    17581 caaaacccat accgtccgca aaggcgaaac ggtcaaacag attgccgccg ccatccgccc
    17641 gaaacacctg acgctcgaac aggttgccga tgcgctgctg aaggcaaacc caaatgtttc
    17701 cgcacacggc agactgcgtg cgggcagcgt gcttcacatt ccgaatctga acaggatcaa
    17761 agcggaacaa cccaaaccgc aaacggcgaa acccaaagcc gaaaccgcat ccatgccgtc
    17821 cgaaccgtcc aaacaggcaa cggtagagaa accggttgaa aaacctgaag caaaagttgc
    17881 cgcgcccgaa gcaaaagcgg aaaaaccggc cgttcgaccc gaacctgtac ccgctgcaaa
    17941 tactgccgca tcggaaaccg ctgccgaatc cgccccccaa gaagccgccg cttctgccat
    18001 cgacacgccg accgacgaaa ccggtaacgc cgtttccgaa cctgtcgaac aggtttctgc
    18061 cgaagaagaa accgaaagcg gactgtttga cggtctgttc ggcggttcgt acaccttgct
    18121 gcttgccggc ggaggcgcgg cattaatcgc cctgctgctg cttttgcgcc ttgcccaatc
    18181 caaacgcgcg cgccgtaccg aagaatccgt ccctgaggaa gagcctgacc ttgacgacgc
    18241 ggcagacgac ggcatagaaa tcacctttgc cgaagtcgaa actccggcaa cgcccgaacc
    18301 cgctccgaaa aacgatgtaa acgacacact tgccttagat ggggaatctg aagaagagtt
    18361 atcggcaaaa caaacgttcg atgtcgaaac cgatacgcct tccaaccgca tcgacttgga
    18421 tttcgacagc ctggcagccg cgcaaaacgg cattttatcc ggcgcactta cgcaggatga
    18481 agaaacccaa aaacgcgcgg atgccgattg gaacgccatc gaatccacag acagcgtgta
    18541 cgagcccgag accttcaacc cgtacaaccc tgtcgaaatc gtcatcgaca cgcccgaacc
    18601 ggaatctgtc gcccaaactg ccgaaaacaa accggaaacc gtcgataccg atttctccga
    18661 caacctgccc tcaaacaacc atatcggcac agaagaaaca gcttccgcaa aacctgcctc
    18721 accctccgga ctggcaggct tcctgaaggc ttcctcgccc gaaaccatct tggaaaaaac
    18781 agttgccgaa gtccaaacac cggaagagtt gcacgatttc ctgaaagtgt acgaaaccga
    18841 tgccgtcgcg gaaactgcgc ctgaaacgcc cgatttcaac gccgccgcag acgatttgtc
    18901 cgcattgctt caacctgccg aagcaccgtc cgttgaggaa aatataacgg aaaccgttgc
    18961 cgaaacaccc gacttcaacg ccaccgcaga cgatttgtcc gcattacttc aaccttctaa
    19021 agtacctgcc gttgaggaaa atgcagcgga aaccgttgcc gatgatttgt ccgcactgtt
    19081 gcaacctgct gaagcaccgg ccgttgagga aaatgtaacg gaa
//
LOCUS       DAWHSK010000012        18295 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.012, whole genome shotgun sequence.
ACCESSION   DAWHSK010000012 DAWHSK010000000
VERSION     DAWHSK010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18295
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.012"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..1319)
                     /locus_tag="WIC03_000315"
     CDS             complement(<1..1319)
                     /locus_tag="WIC03_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor"
                     /protein_id="HEZ3882876.1"
                     /translation="MHDTRTMMIKPTALLLPALFFFPHAYAPAADLSENKAAGFALFK
                     NKSPDTESVKLKPKFPVRIDTQDSEIKDMVEEHLPLITQQQEEVLDKEQTGFLAEEAP
                     DNVKTMLRSKGYFSSKVSLTEKDGAYTVHITPGPRTKIANVGVAILGDILSDGNLAEY
                     YRNALENWQQPVGSDFDQDSWENSKTSVLGAVTRKGYPLAKLGNTRAAVNPDTATVDL
                     NVVVDSGRPIAFGDFEITGTQRYPEQIVSGLARFQPGTPYDLDLLLDFQQALEQNGHY
                     SGASVQADFDRLQGDRVPVKVSVTEVKRHKLETGIRLDSEYGLGGKIAYDYYNLFNKG
                     YIGSVVWDMDKYETTLAAGISQPRNYRGNYWTSNVSYNRSTTQNLEKRAFSGGIWYVR
                     DRAGIDARLGAEFLAEGRKIPGSDIDLGNSHATMLTASWKRQLLNNVL"
     gene            1695..2924
                     /gene="sstT"
                     /locus_tag="WIC03_000316"
     CDS             1695..2924
                     /gene="sstT"
                     /locus_tag="WIC03_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HEZ3882877.1"
                     /translation="MAFGKSLFHAIGRVSLVRQIAAGLALGIVIGSVSPQLGLAAGLF
                     GSLFVGALKAVAPVLVFILVAATIAQHQKGNKAHIRPIIVLYLIGTFSAALTAVIAGM
                     VFPTHIVLAGAGDVSAAPPSGIVEVLKSLLMNLVANPINAIANANYIGILAWALVLGA
                     ALRNHGSDVTRQVVADLAEAVSTVVKWIIRFAPLGIFGLVSSTIAETGFGALAGYAKL
                     LAVLLGCMAFIALVVNPAIVWWKIRRNPYPLVFTCLRESGVYAFFTRSSAANIPVNMA
                     LAKKLGLHEDTYSISIPLGATVNMGGAAITITVLAMAAAHTQGIQVDFATALLLSLVA
                     TVSACGASGVAGGSLLLIPLACSLFGISNDVAMQVVAVGFIIGVIQDSAETALNSSTD
                     VLFTAAADLGRQRNRAE"
     gene            3041..4519
                     /locus_tag="WIC03_000317"
     CDS             3041..4519
                     /locus_tag="WIC03_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ3882878.1"
                     /translation="MFKRSVIAMACIFALSACGGGGGGSPDVKSADTLSKPAAPVVSE
                     KETEAKEDAPQAGSQGQGAPSAQGGQDMAAVSEENTGNGGAAATDKPKNEDEGAQNDM
                     PQNAADTDSLTPNHTPASNMPAGNMENQAPDAGESEQPANQPDMANTADGMQGDDPSA
                     GGENAGNTAAQGTNQAENNQTAGSQNPASSTNPSATNSGGDFGRTNVGNSVVIDGPSQ
                     NITLTHCKGDSCSGNNFLDEEVQLKSEFEKLSDADKISNYKKDGKNDGKNDKFVGLVA
                     DSVQMKGINQYIIFYKPKPTSFARFRRSARSRRSLPAEMPLIPVNQADTLIVDGEAVS
                     LTGHSGNIFAPEGNYRYLTYGAEKLSGGSYALSVQGEPAKGEMLAGTAVYNGEVLHFH
                     TENGRPYPSRGRFAAKVDFGSKSVDGIIDSGDDLHMGTQKFKAAIDGNGFKGTWTENG
                     SGDVSGRFYGPAGEEVAGKYSYRPTDAEKGGFGVFAGKKEQD"
     gene            complement(4595..4810)
                     /locus_tag="WIC03_000318"
     CDS             complement(4595..4810)
                     /locus_tag="WIC03_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3882879.1"
                     /translation="MFKLGVYACLGLFAGWVLLLIVQLWFSFLEAELFFKITLTMAGL
                     FVIILAALLVCGQYFSEKKMKDDGFIN"
     gene            complement(4803..5018)
                     /locus_tag="WIC03_000319"
     CDS             complement(4803..5018)
                     /locus_tag="WIC03_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HEZ3882880.1"
                     /translation="MKEYKVVIYQESLLSSLFFGAAKVNPVNFSAFLNKQTPEGWRVV
                     TMEKDLRRMLLFFKREAYVVILERDRV"
     gene            complement(5093..6436)
                     /gene="argG"
                     /locus_tag="WIC03_000320"
     CDS             complement(5093..6436)
                     /gene="argG"
                     /locus_tag="WIC03_000320"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEZ3882881.1"
                     /translation="MNQNHTILQNLPIGQKVGIAFSGGLDTSAALLWMKLKGALPYAY
                     TANLGQPDEDDYNAIPKKAMEYGAENARLIDCRAQLAHEGIAAIQCGAFHVSTGGIAY
                     FNTTPLGRAVTGTMLVSAMKEDDVNIWGDGSTYKGNDIERFYRYGLLTNPALKIYKPW
                     LDQQFIDELGGRHEMSEFLIANGFNYKMSVEKAYSTDSNMLGATHEAKDLEFLNSGIK
                     IVKPIMGVAFWDENVEVSPEEVSVRFEEGVPVALNGKEYADPVELFLEANRIGGRHGL
                     GMSDQIENRIIEAKSRGIYEAPGMALFHIAYERLVTGIHNEDTIEQYRINGLRLGRLL
                     YQGRWFDSQALMLRETAQRWVAKAVTGEVTLELRRGNDYSILNTESPNLTYQPERLSM
                     EKVEDAAFTPLDRIGQLTMRNLDITDTRTKLSIYSQSGLLALGEGSVLPQLGNKQ"
     gene            6900..7700
                     /locus_tag="WIC03_000321"
     CDS             6900..7700
                     /locus_tag="WIC03_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEZ3882882.1"
                     /translation="MNAFNLIIIGDEILHGSRQDKHFAFFKSLLESKGLKLNQVQYLP
                     DEPDLLVRQLRRSFSDGIPTFVTGGIGATPDDHTRQAAAAALELPVVRHPEAAGFIEG
                     ITQKRGEPLDSPEHAQRLKMADFPEGAELVPNPFNNIAGFSIREHYFFPGFPVMAHPM
                     AEWVLETYYADRFNQTERGSRSVYVFEQPESRITPLMEYIEQTYLGVRSYSLPSVGWT
                     HSDGTQVKPHIEFGIKAEGEAVNLLDAAWAEVLHSLEGLGAELKNRVN"
     gene            7740..8726
                     /locus_tag="WIC03_000322"
     CDS             7740..8726
                     /locus_tag="WIC03_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HEZ3882883.1"
                     /translation="MQYRIRRENEAPEAKNAGETLWERDMMREVLLSAYRDRRRERMW
                     KNIWRAVSTLILVALIAGIFRKDEAALQLAGNTPHTAVVNLYGEIGNGVEDQVKKLKD
                     GMEAAYKNPQAKAIIIRANSPGGSPVVSNTAFEEIRRLKAQHPGIPVYLVAEDMCASG
                     CYYIAAAADKIYADPSSIIGSIGVIGSSFDATGLMEKIGVKRRVKIAGSNKGMGDPFS
                     PETPEQSKIWEEMLTGIHGEFIKAVKTGRGDRLKFRQYPDVFSGRVYTGADALKVGLV
                     DGLGNIYSVARDVVKAPDVVDYTPKDDFGRILGRRFGAELKASVREALQAVR"
     gene            9383..9667
                     /locus_tag="WIC03_000323"
                     /pseudo
     CDS             9383..9667
                     /locus_tag="WIC03_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805306.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(9705..10079)
                     /locus_tag="WIC03_000324"
     CDS             complement(9705..10079)
                     /locus_tag="WIC03_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3882884.1"
                     /translation="MIIQNEFNLYPSNMLPERFCYPEKYVRISNDTSLIPYIQPHNFH
                     WWFENYGTEGAEVAYIFRNSILPDLNLIPFASNGEWEAYFDGNDVTGNPRVIVINLDN
                     IENHEFFNSFEDWLELAIKDTW"
     gene            complement(10252..10608)
                     /locus_tag="WIC03_000325"
     CDS             complement(10252..10608)
                     /locus_tag="WIC03_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mimic protein"
                     /protein_id="HEZ3882885.1"
                     /translation="MNEHNLLIFCLKDNVSISEYTEMIDWAYKNIQSETVVEITESQI
                     IEYQNRGLWRLFSEITDNWLFGPSEGDWLIDKESILAVKEKLQNSDFSTEPLVKNIIH
                     VLEYAIKNEKTVIFHF"
     gene            complement(10751..11095)
                     /locus_tag="WIC03_000326"
     CDS             complement(10751..11095)
                     /locus_tag="WIC03_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3882886.1"
                     /translation="MNLENYENILIKLLFYHNNLVNEYSYFIENKNLFKIVSRTKTSK
                     GFFFTIEKPLNFLSKKTYFEFNFKYLHSGKERFGSFMCWINTNLMEFEGVFFNDLLPD
                     NMIINNFFEIND"
     gene            complement(11254..11655)
                     /locus_tag="WIC03_000327"
     CDS             complement(11254..11655)
                     /locus_tag="WIC03_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3882887.1"
                     /translation="MKLKSLDFPTGYFYFDNAAINSDKVEVIAVGYRNTDKTIKIFIE
                     DVIHFRVVDESYFIDTFMDLISEDADRALLHENGGQSFFELLDECYAEWILKESYFPL
                     NREFFKYYIFMFEQTFIEIIGSSATYSIIEG"
     gene            complement(11659..12399)
                     /locus_tag="WIC03_000328"
                     /pseudo
     CDS             complement(11659..12399)
                     /locus_tag="WIC03_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713004.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(12469..12786)
                     /locus_tag="WIC03_000329"
     CDS             complement(12469..12786)
                     /locus_tag="WIC03_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3882888.1"
                     /translation="MKKNIFHNVSLYEIIFSDNGNTLTLSFTDTIEGNYFGYIKCSNI
                     LNFKLDTNNFVDYEDKEDSLFPLFIPEIELYKYQFYSEIIIDVGIIIKISAETINFEP
                     LGK"
     gene            complement(12783..13385)
                     /locus_tag="WIC03_000330"
                     /pseudo
     CDS             complement(12783..13385)
                     /locus_tag="WIC03_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253822.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(13811..14155)
                     /locus_tag="WIC03_000331"
     CDS             complement(13811..14155)
                     /locus_tag="WIC03_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3882889.1"
                     /translation="MIIEHNGNIHKIARMTGNKNNFLEIILSDIHENIKIKPLTIKVK
                     GENVINILPEEVSFYVKQGVDLIYEKYKRKFFISEISFCQSDSRPSSIYAFLTFHLLE
                     DIIKNESPSNYT"
     gene            complement(14193..14537)
                     /locus_tag="WIC03_000332"
     CDS             complement(14193..14537)
                     /locus_tag="WIC03_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3882890.1"
                     /translation="MFLDDVNVFLDDLNVFLDDLNTNPITDEWYMSNFADKHIKILES
                     YEAFDILKQFVDYMIEEYDEKSEYEIMEILRQLKYQADTNEKFYTNTQKQKIVELYKQ
                     EISQDILNEIFR"
     gene            complement(14540..14728)
                     /locus_tag="WIC03_000333"
     CDS             complement(14540..14728)
                     /locus_tag="WIC03_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mafB"
                     /protein_id="HEZ3882891.1"
                     /translation="MEEVKANPQGKTPARIPPMSDTKNGWLAKDGWVKRVQNVNKIEI
                     HYIENSRTGEKTDFKFKD"
     gene            complement(14798..15142)
                     /locus_tag="WIC03_000334"
                     /pseudo
     CDS             complement(14798..15142)
                     /locus_tag="WIC03_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503511.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(15226..15528)
                     /locus_tag="WIC03_000335"
     CDS             complement(15226..15528)
                     /locus_tag="WIC03_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3882892.1"
                     /translation="MLNEIFEIYSRQGESLIGIGIREAALPVPIAIDILNLFINERIL
                     VLGGDIYIKKDNYFYQTYDNWYYEGSNLFNSIDKAMHYLSQIKLENAYVSFVLKFI"
     gene            complement(15551..15820)
                     /locus_tag="WIC03_000336"
     CDS             complement(15551..15820)
                     /locus_tag="WIC03_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3882893.1"
                     /translation="MMTESHGYLSNNLPVKIINDIIYATQLVEDLVLGKIKIVDFLKS
                     YNNFYCWLGFDELPQSEKIKFLSYLNILSIHKEIQDETVNRVYTD"
     gene            complement(15841..16047)
                     /locus_tag="WIC03_000337"
     CDS             complement(15841..16047)
                     /locus_tag="WIC03_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3882894.1"
                     /translation="MMENSKKTWEIDGEIWLHCPVCGTEVMDYDICDVCQWQNTGETN
                     IDGGPNEMTLAEAKEAYAKGLPIR"
     gene            complement(16013..16762)
                     /gene="mafB"
                     /locus_tag="WIC03_000338"
                     /pseudo
     CDS             complement(16013..16762)
                     /gene="mafB"
                     /locus_tag="WIC03_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257312.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
     gene            complement(16868..17239)
                     /locus_tag="WIC03_000339"
     CDS             complement(16868..17239)
                     /locus_tag="WIC03_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEZ3882895.1"
                     /translation="MIKQNSFVPYPEAMLPKGFKYPQSYLKLAQSTHAINYDEQYSFP
                     WWFENAESNISEVIDIYFEITGIPNLLPFARNQEWAACFDISDKSGNPKIIVVNLDNT
                     KYYETFENFDTWLKEAENDGW"
     gene            complement(17236..17487)
                     /locus_tag="WIC03_000340"
     CDS             complement(17236..17487)
                     /locus_tag="WIC03_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3882896.1"
                     /translation="MDNTRIMAAREAGVKVEANVHNFNDRLSSKERIRFKHDGIEPQT
                     WGEAIQLRIRKQETQKGVPEGWSKRFPNGSIYDVKVLRK"
     gene            complement(17619..17825)
                     /locus_tag="WIC03_000341"
     CDS             complement(17619..17825)
                     /locus_tag="WIC03_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3882897.1"
                     /translation="MNILSINNQNSTISLTQDEVFVLRAILNEIYAGVCVDSREFENV
                     SGVRKHEVDNLQQQFAGIYKKMTT"
     gene            complement(17835..>18295)
                     /gene="mafB"
                     /locus_tag="WIC03_000342"
     CDS             complement(17835..>18295)
                     /gene="mafB"
                     /locus_tag="WIC03_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HEZ3882898.1"
                     /translation="GKKVELNPTKWDWVKNTGYKKPAARHMQTVDGEMAGGNRPPKSI
                     TSNSKADASTQPSLQAQLIGEQISSGHAYNKHVIRQQEFTDLNINSPADFARHIENIV
                     SHPTNMKELPRGRTAYWDDKTGTIVIRDKNSDDGGTAFRPTSGKKYYDDL"
BASE COUNT         4698 a         4052 c         4131 g         5414 t
ORIGIN      
        1 agcacgttgt tgagcagctg gcgtttccaa gaggcggtca gcatcgtggc gtggctgttg
       61 cccaaatcga tatccgagcc gggaattttc cggccttctg cgagaaactc tgcccccagc
      121 ctggcatcga tgcccgcgcg gtcgcgcaca taccagatgc cgccggagaa ggcgcgtttt
      181 tcgaggtttt gggtggtcga acggttgtag gaaacgttgc ttgtccagta gttgccccga
      241 tagttgcgcg gctggctgat gccggcggca agcgtggttt cgtatttgtc catatcccag
      301 acgaccgagc cgatatagcc tttgttgaag aggttgtaat agtcgtaggc gattttgccg
      361 cccaaaccgt attccgaatc gaggcggatg ccggtttcga gcttgtggcg tttgacctcg
      421 gttacgctga ctttgacggg gacgcggtcg ccttggagac ggtcgaagtc ggcttgtacg
      481 gacgcgccgg aataatgccc gttttgttcg agtgcctgtt ggaagtcgag cagcaggtcg
      541 aggtcgtagg gcgtgcccgg ttggaagcgc gccaggccgg agacgatttg ttcggggtaa
      601 cgctgtgtgc cggtgatttc aaagtcgccg aaggcgatgg ggcggccgct gtccacgacg
      661 acgttcaaat cgacggtggc ggtatcgggg ttgacggccg cccgggtgtt gccgagcttg
      721 gcaagcgggt agcctttgcg cgttaccgcg ccgaggacgg aagttttgct gttttcccaa
      781 ctgtcctgat cgaaatcgct gcctaccggc tgctgccagt tttccagcgc gttgcggtag
      841 tattcggcga ggttgccgtc tgaaaggatg tcgccgagga tggcgacgcc gacgttggcg
      901 attttggtgc gcgggcccgg tgtgatgtgc accgtataag ctccgtcttt ttccgtcaga
      961 ctgactttgc tgctgaaata gcctttgctg cggagcatcg ttttaacgtt gtccggtgct
     1021 tcttcggcga ggaagcctgt ctgttcctta tccaaaacct cttcctgctg ctgcgtgata
     1081 agcggcaggt gttcttcgac catatctttg atttcactgt cctgcgtgtc gatgcggacg
     1141 gggaatttgg gttttaattt aactgattcg gtgtcagggc ttttgttttt gaacaatgcg
     1201 aaacccgccg ccttgttttc ggaaaggtcg gcggcaggcg cgtatgcgtg cggaaagaaa
     1261 aataaagccg gcaggagcag ggcggtcggt ttgatcatca tggtacgggt gtcgtgcatc
     1321 ggtgtggcgg atttcgattc taacactgtt cggacggggc gggacgcttc agacggcata
     1381 ctgccgccgg tatttcggag gttgcggccc gccggaaatg tatctgcttg ttttagggta
     1441 ttttgtttct aatccgtata tggaaaacag gctgcgtttc ggaatagtgc cggaaaatag
     1501 gcaggattaa ataaatttat tttcgttcaa taaattaaac cgttgcgccg aatgataaag
     1561 caaagcctgt ccggcactgc atagggcaaa tcaatgcaac cttaaaacac agccgaaaaa
     1621 taaaaaaaac acagagaatg agttttcttt taaaatacaa cattttttaa catatcacaa
     1681 cataaggaag aattatggcg ttcggcaaat ccttatttca tgcgatcggc cgcgtcagcc
     1741 tggtcaggca gattgccgcc ggtttggcgt tgggtatcgt aatcggttcg gtttccccgc
     1801 aactgggctt ggcggcaggc ttgttcggca gcctgtttgt cggcgcgctc aaagcggtcg
     1861 cgccggtttt ggtatttatt ttggtggcgg ccacaatcgc gcagcaccaa aaaggcaaca
     1921 aggcgcatat caggccgatt atcgtccttt acctcatcgg tacgttttcc gcagccctga
     1981 ccgccgtcat cgctggtatg gttttcccga cgcacattgt tttggcgggc gcgggcgatg
     2041 tgtccgccgc gccgccttcc ggcattgtgg aagtattgaa atcgctgctg atgaacctgg
     2101 tcgccaaccc gattaacgcg attgccaatg ccaactatat cggcattttg gcttgggctt
     2161 tggttttggg cgcggcgttg cggaatcacg gttcggacgt tacgcggcag gtcgttgccg
     2221 atttggcgga agcggtttcc accgtcgtga aatggattat ccgttttgcc cctttgggca
     2281 ttttcgggct ggtgtcgtcc acaatcgcgg agacgggttt cggcgcgctg gcgggttatg
     2341 cgaagctgct tgcggtactg ttgggctgta tggcgtttat cgcgctggtg gtcaatcccg
     2401 ccatcgtgtg gtggaaaatc cgccgcaacc cttatccgct ggtgtttacc tgcctgcgcg
     2461 aaagcggcgt gtatgccttc tttacccgtt cttccgccgc caatatcccc gtgaatatgg
     2521 ctttggcgaa aaaactgggg ctgcacgaag acacttattc gatttccatc ccgttggggg
     2581 cgaccgtcaa tatgggcggc gcggcgatta cgattaccgt tttggctatg gcggcggcgc
     2641 acacccaagg catacaggtt gattttgcca ccgcgctgct gttgagcctg gtggcgacgg
     2701 tcagcgcgtg cggcgcgtcc ggtgtggcag gcggttcgct gctgctgatt ccgctggcgt
     2761 gcagcctgtt cggcatcagc aacgatgtcg ctatgcaggt ggtcgccgtc ggctttatca
     2821 tcggcgtgat tcaggattcg gcggaaacgg cgttgaattc ttcaaccgac gttttgttta
     2881 ccgctgccgc cgatttgggc cgacagagaa accgggcgga ataaaccaag ctatgccgtc
     2941 tgaagcccgt tcggcgttca gacggcatat ttttacaatt ttataggcat acggcggaaa
     3001 caatgccggc tttgctttat tgaatacgaa ggagatgatg atgtttaaac gcagcgtaat
     3061 cgcaatggct tgtatttttg ccctttcagc ctgcgggggc ggcggtggcg gatcgcccga
     3121 tgtcaagtcg gcggacacgc tgtcaaaacc tgccgcccct gttgtttctg aaaaagagac
     3181 agaggcaaag gaagatgcgc cacaggcagg ttctcaagga cagggcgcgc catccgcaca
     3241 aggcggtcaa gatatggcgg cggtttcgga agaaaataca ggcaatggcg gtgcggcagc
     3301 aacggacaaa cccaaaaatg aagacgaggg ggcgcaaaat gatatgccgc aaaatgccgc
     3361 cgatacagat agtttgacac cgaatcacac cccggcttcg aatatgccgg ccggaaatat
     3421 ggaaaaccaa gcaccggatg ccggggaatc ggagcagccg gcaaaccaac cggatatggc
     3481 aaatacggcg gacggaatgc agggtgacga tccgtcggca ggcggggaaa atgccggcaa
     3541 tacggctgcc caaggtacaa atcaagccga aaacaatcaa accgccggtt ctcaaaatcc
     3601 tgcctcttca accaatccta gcgccacgaa tagcggtggt gattttggaa ggacgaacgt
     3661 gggcaattct gttgtgattg acgggccgtc gcaaaatata acgttgaccc actgtaaagg
     3721 cgattcttgt agtggcaata atttcttgga tgaagaagta cagctaaaat cagaatttga
     3781 aaaattaagt gatgcagaca aaataagtaa ttacaagaaa gatgggaaga atgacgggaa
     3841 gaatgataaa tttgtcggtt tggttgccga tagtgtgcag atgaagggaa tcaatcaata
     3901 tattatcttt tataaaccta aacccacttc atttgcgcga tttaggcgtt ctgcacggtc
     3961 gaggcggtcg cttccggctg agatgccgct gatccccgtc aatcaggcgg atacgctgat
     4021 tgtcgatggg gaagcggtca gcctgacggg gcattccggc aatatcttcg cgcccgaagg
     4081 gaattaccgg tatctgactt acggggcaga aaaattgtcc ggaggatcgt atgccctcag
     4141 tgtgcaaggc gaaccggcaa aaggcgaaat gcttgctggc acggccgtgt acaacggcga
     4201 agtgctgcat ttccatacgg aaaacggccg tccgtacccg tccagaggca ggtttgccgc
     4261 aaaagtcgat ttcggcagca aatctgtgga cggcattatc gacagcggcg atgatttgca
     4321 tatgggtacg caaaaattca aagccgccat cgatggaaac ggctttaagg ggacttggac
     4381 ggaaaatggc agcggggatg tttccggaag gttttacggc ccggccggcg aggaagtggc
     4441 gggaaaatac agctatcgcc cgacagatgc ggaaaagggc ggattcggcg tgtttgccgg
     4501 caaaaaagag caggattgat gttgatgccg tctgaagcgg gggcgttcag acggcattgt
     4561 gatgtttggg tcgcgggtcc ggttcaagtc cgcatcagtt gataaacccg tcgtctttca
     4621 ttttcttttc ggaaaaatac tggccgcata ccagcagggc ggcgaggatg atgacaaaca
     4681 gccccgccat agtcagtgtg attttgaaga acaattcggc ttcgagaaaa gaaaaccaaa
     4741 gttgcacaat cagcagcagc acccagccgg caaacagtcc gagacaggca taaacgccga
     4801 gcttaaacac ggtcccgctc caaaatgacg acgtaggcct cgcgtttgaa aaacaacagc
     4861 atacggcgca aatctttttc catcgttaca acccgccagc cttcgggggt ttgtttgttg
     4921 aggaacgcgc tgaaattgac ggggttgacc tttgccgcgc cgaaaaacag gctggacaac
     4981 aggctttcct gataaatgac gactttgtat tctttcatag gattcctttt aacataaggg
     5041 atcatctttt cagacgaccc cttaagttgc taatgatgta aaacagcaaa ccttattgct
     5101 tattgcccaa ctgcggcaat accgagcctt cgcccaatgc caacaaaccg ctttgcgagt
     5161 aaatactcag tttggtgcgg gtgtcggtga tgtcgaggtt gcgcatcgtg agttgtccga
     5221 tacggtcgag cggtgtgaac gccgcatctt cgactttttc catactcagg cgttcaggtt
     5281 ggtaggtcag gttgggcgat tcggtgttca gaattgagta gtcgttgccg cgccgcagtt
     5341 cgagggtaac ttcgccggta acggctttag cgacccagcg ttgggcggtt tcgcgcaaca
     5401 tcagggcttg gctgtcgaac cagcggcctt ggtagagcaa acgtccgagg cgcaggccgt
     5461 tgatgcggta ttgttcgatg gtgtcctcgt tgtggatgcc ggttaccaag cgttcgtagg
     5521 cgatgtggaa caacgccatg cccggggctt cgtagatgcc gcgcgattta gcttcgatga
     5581 tgcggttttc gatttggtcg ctcataccta agccgtggcg gccgccgatg cggttggctt
     5641 cgaggaagag ttcgacgggg tcggcgtatt ctttgccatt cagtgcaacc ggcacgcctt
     5701 cttcaaagcg cacgctgact tcttcggggc tgacttcgac gttttcgtcc caaaaggcaa
     5761 cacccataat gggtttgacg attttgatgc ccgagttcaa aaattccaag tctttggctt
     5821 cgtgggtcgc gccgagcata ttggaatcgg tggaataagc tttttcaacc gacattttgt
     5881 agttgaagcc gttggcaatc agaaattcgc tcatttcgtg gcggccgccg agttcgtcga
     5941 taaattgctg gtcgagccag ggtttgtaga ttttcagcgc gggattggtg agcaaaccgt
     6001 agcggtagaa acgctcgatg tcgttgcctt tgtaggtgct gccgtcgccc caaatattca
     6061 catcgtcttc tttcattgcg gaaacaagca tcgtgccggt tacggcgcgg cccagaggcg
     6121 tggtgttgaa ataggcgatg ccgccggtgg aaacgtggaa cgcgccgcat tggatggcgg
     6181 cgatgccttc atgtgccaac tgcgcgcggc agtcgattaa gcgggcgttt tccgcaccgt
     6241 attccatcgc ttttttggga atggcgttgt agtcgtcttc atcgggctga ccgaggttgg
     6301 cagtgtaggc ataaggcagt gcgcctttga gtttcatcca caacagcgcg gcggaggtat
     6361 caagtccgcc ggagaaggcg atgccgactt tctgaccgat ggggaggttt tgtaaaatag
     6421 tatggttttg gttcataagg gtatccttcg gacttggaaa aaggagttca tcgtgtttcc
     6481 ttttcggttg aaaccccgcc acttggacat ctgtccttcg gggcggtaga atcagacctt
     6541 atttgggagg ggtgtaaccc cttccaaatc aggacggcac atagggcggt gctttatgtg
     6601 tcgtcccgtg tgttgaaaca ttatcttata aatataaccc attgtcagcc gttaaggctg
     6661 tctgaaaaga agctttgccc gtttcgctga aaatcttttc agacggcatt tacgccatcc
     6721 tcaacctgac tccctcccct gcgggggagg gttggggaga gggtgcagac agcagccctt
     6781 aagcaaacgt tgcctgaaaa acgcaagctc gaccctctcc ctaaccctct cccacgggag
     6841 agggaacaaa tcacagcact atcgacattt tcagacggca tcggtaaaga aacccatcta
     6901 tgaacgcgtt caacctcatc atcatcggcg acgaaatcct gcacggcagc cgccaagaca
     6961 agcatttcgc ctttttcaaa tccctgctcg aatccaaagg gctgaagctc aatcaggtgc
     7021 aatacctgcc cgacgaaccc gatttgctgg tcaggcaact gcgccgcagc ttttcagacg
     7081 gtataccgac gtttgttacc ggcggcatcg gcgccacgcc cgacgaccac acgcgccaag
     7141 cggccgccgc cgctttggag ttgcccgtcg tccgccatcc tgaagccgcc gggtttatcg
     7201 aaggcataac ccagaaacgc ggcgagccgc ttgattcgcc ggaacacgcc caacgcctga
     7261 aaatggcaga ttttcccgaa ggcgcggaat tggtgcccaa tccgtttaac aacatcgccg
     7321 gattttccat ccgcgagcat tattttttcc ccggcttccc cgtgatggcg cacccgatgg
     7381 cggaatgggt attggaaact tattacgccg accgcttcaa ccaaaccgaa cgcggcagcc
     7441 gcagcgtgta tgtgttcgag cagcccgaat cgcgcattac gccgcttatg gagtatatcg
     7501 agcaaactta tctcggggtg cgttcgtaca gcctgcccag cgtcggttgg acgcattcag
     7561 acggcacgca ggtcaaaccg cacattgagt tcggcatcaa agccgaagga gaagccgtca
     7621 accttttgga cgcggcgtgg gcggaagtgt tgcacagctt ggaaggattg ggagcagagc
     7681 tgaaaaatcg ggtaaactga aaaacgtttg cccaaatcgg acgatattta aggaaaacca
     7741 tgcaataccg aatcagaaga gaaaacgaag cacccgaagc aaaaaatgcg ggggaaacct
     7801 tatgggagcg cgatatgatg cgcgaagtgc tgctgtccgc ctatcgggac aggcgcaggg
     7861 agcggatgtg gaaaaacatc tggcgcgccg tcagcaccct gattctggtt gccctgattg
     7921 caggcatttt ccgaaaagac gaagccgcat tgcagttggc gggcaatacg ccgcataccg
     7981 cagtcgtcaa cctgtacggc gaaatcggca acggtgtaga agaccaggtc aaaaaactca
     8041 aagacggtat ggaggccgcc tacaaaaacc cgcaggcaaa agccatcatc atccgcgcca
     8101 acagtccggg cggctctccc gtcgtatcca atactgcctt tgaggaaata cgccgtctga
     8161 aagcgcaaca tcccggcatt cccgtttatc tcgtggcgga agatatgtgc gcgtccggct
     8221 gctactacat cgcggcggcg gcggacaaaa tctatgccga cccgtccagc atcatcggca
     8281 gcatcggcgt tatcggcagc agtttcgacg cgaccggcct gatggaaaaa atcggcgtga
     8341 aacgcagggt taaaattgca ggcagcaaca aaggcatggg cgatccgttt tcgcccgaaa
     8401 cgcccgaaca gtcgaaaatc tgggaagaaa tgctgaccgg catacacggc gagttcatca
     8461 aagccgtcaa aaccggacgc ggcgaccgcc tcaaattccg acaatatccc gacgtgttca
     8521 gcggtcgcgt gtacacaggc gcggatgcgc tgaaagtcgg gctggtggac ggactcggca
     8581 atatttacag cgtggcgcga gacgtggtca aagcccccga tgtggtggat tacactccga
     8641 aggacgattt cggcagaatc ttgggcagac gcttcggcgc agaactcaaa gcaagcgtgc
     8701 gcgaagccct gcaggccgtc cggtagcgtt gtttacagta tttctaaggg aataccaaaa
     8761 taaaaatata cacaaaaaca ctcacctccc cagtatgctg ccactaagtc agccgcgtca
     8821 ctatttaccg tgcacttaaa gccgtaaaag gcaggttgtc cccgggaggt tttgtgcatc
     8881 ctgataccga tcggttgctg ttgctcaaag gacagaaggc cgctgataaa cgagattacc
     8941 tgtttgtcgc tattgacgat ttttatactc tgccattttg ccggacaaaa ccgcagacag
     9001 tgctgccaag tttctgaccg aacatctggc cgacccctgc ttgtacctga ttgagtacgt
     9061 ttactctgac aatgataggt aatataaaga gccgtccaac atgctttcgg tgcagcttgt
     9121 tataataatg ggattggttg gaggcttgcc cgatttgctt gtccgcagac caacggtaag
     9181 gcggagcggg ttatccgtac cttgatggag atgtggcatg aggaacagtc gtttgacagt
     9241 ccggaacatc ggcaaaagga gttgtgctgc tttattaatt taggggctgc cctagatcac
     9301 tagggaaatt caaattaagt tagaattatc cctatgagag aaagtcgtct aagccggtat
     9361 aaacaaaata aactcatttt gttatcgtgg ctatgatgta ttggatgcgg gcgaatttag
     9421 ccattttagc ttcgctgaaa cttcgttttc gtatcaatca cagcacacat tttgccgaac
     9481 gacaaaacca tattaatgga actgagaact tttggaacca ggcaaaacgt catttacgca
     9541 agtttaacgg cattcccaaa gcgcattttg agctgtattt aaaggagcgc gaatggcgtt
     9601 ttaacaacag tgagataaaa gttcttgttc cattttaaaa caattagtaa aatcgagttt
     9661 atcctagttg tccagggcag cctcatctct ttatagatag ctgcttacca agtatcctta
     9721 attgctaatt caagccaatc ttcaaaacta ttaaaaaatt catggttttc tatattatct
     9781 aaattaatga caataaccct aggatttcct gttacatcat taccatcaaa ataagcttcc
     9841 cattctccat tactagcgaa tgggataaga tttaaatcag gcaggataga atttctaaat
     9901 atataagcta cttctgcccc ttctgttcca taattctcaa accaccagtg aaaattatgt
     9961 ggttgaatat aaggtattaa agatgtatcg ttagagatac gaacatactt ttcaggataa
    10021 caaaaccttt caggaagcat attactagga tataaattaa attcattttg aataatcatt
    10081 tcgttacctt aggatcataa atactaccat taggaaactg ggtagaccaa tttggtggtg
    10141 ctagttcatt ttcaatttgt tttttaattc taaattgaat agcttctccc catgtttgcg
    10201 gcatctaatg ctgcttgctc tgcatcaata ttactaaaaa ttggattagt ctcaaaaatg
    10261 aaaaattact gttttttcat tttttatagc atattcaagt acatgaataa tatttttcac
    10321 taagggctct gtagaaaaat ctgaattttg taatttttct tttacagcca aaatactttc
    10381 cttatctatt agccaatccc cctcacttgg tccaaataac caattatcgg taatttcaga
    10441 aaaaagtctc cataatccac gattttgata ttcaataatt tgactttccg taatttctac
    10501 aactgtttca gattgaatgt ttttataagc ccaatcaatc atttcagtat attcactaat
    10561 tgaaacattg tcttttaaac agaaaattaa caggttgtgt tcattcattt gtctaagttc
    10621 ttaatatgag tttgttgttg taaataatct tttgcttgct taccttgtaa attaaaggat
    10681 taaccgcacc ttggatgatg ccgcgaatat catccatacg gttaccccac caaattttat
    10741 tggtgtatcg ttaatcattt atttcaaaaa agttatttat tatcatatta tcagggagca
    10801 ggtcgttaaa aaaaaccccc tcaaattcca ttaaattagt atttatccag cacataaacg
    10861 aaccaaagcg ttctttccct gagtgtaaat atttaaaatt aaactcaaaa taagtttttt
    10921 tgcttagaaa atttaatggt ttttctatag tgaaaaaaaa gcccttactc gttttagttc
    10981 gagatactat tttaaataaa tttttatttt caataaaata agaatattca tttactaagt
    11041 tgttatgata aaaaagtaat tttattaaaa tgttttcata attttctagg ttcatttcta
    11101 gctcaattta aaatgatttc attgttgccg taccaatgcg aaatttacca tcaacaaaat
    11161 ttcccttcac tgatacaggt acgccatcaa cttgggtagt tttattttca ggctgcctga
    11221 aagaaagata ggcaacttat tactcatctt acgctagccc tcaataattg aatacgttgc
    11281 actagaacca attatttcta tgaatgtttg ctcaaacata aaaatatagt atttaaagaa
    11341 ttctctattc aaaggaaaat aactttcttt caatatccat tccgcataac actcatcaag
    11401 aagttcaaaa aaagattgac caccattttc atgaagcaaa gctctatctg catcttccga
    11461 aattaaatcc ataaaagtat ctataaaata cgattcatca acaaccctaa aatgaataac
    11521 atcttcaata aaaattttta tggttttatc cgtatttcta taaccaactg ctataacttc
    11581 tactttatca gagtttattg ctgcattatc aaaatagaaa tagccagttg ggaaatctaa
    11641 gctttttaat ttcataattt atcgtgtatt tccatatctg atttttattt ttcggttatt
    11701 actaattgtt atttcaagtg ttggttctcc accactacta tcattacgag cattaaccgc
    11761 acgcctatca ggtaaattgc ccgttatcgt tccattagga cgtgtttgta catttcgaac
    11821 attcagacta ttaaaatcat ttagtgcgtc attgtaatcc cccactttat catactgaac
    11881 agcgggacct tttgttttac tcctttgagt agtattgctc aaaatgtctt tgatttgtaa
    11941 atcactgcgc accttaccgg tttctatgcg agctgccaca ctactcgggc tataggggtc
    12001 gctatcaatc gcaggattac caccgctggt tttttgtccg ctagcctgtt tcccaacctg
    12061 ctgtactgta tcgccaattt catctaccgt acctaacgcc ttagtctgca tatggcgaac
    12121 agcaggtttt ttatagccgg tatttttaac ccaatcccat ttagtcgggt taagttctac
    12181 ttttttacct ctccaaaccg tacctgcggc ctctgctacg gaaagggcag tttgggctgt
    12241 tgcagttata ttcggatggg catcagccca ttgtctggca gagttgatac tgtcttttac
    12301 cgcaaaagca ctgtcttgta atgcggttgc ggccgcaagt tgtgcttcgg gacttaaatt
    12361 tcctaaatta ttaatacctt gtagagtctg ctgcgcagag atgtagcaac tgtattttta
    12421 ccccgtcggg taaaaataca gttgctacgg ctgctgggaa agcagttact attttcccag
    12481 tggctcaaaa ttaattgttt cagcagatat ttttataata atccctacat caataataat
    12541 ttcactataa aattggtatt tatatagctc tatttcgggt ataaacaagg gaaacaagct
    12601 atcttcctta tcctcataat ctacgaaatt atttgtatct aatttaaaat tcaaaatatt
    12661 actgcatttg atatatccga aataattacc ttcaattgta tctgtaaaag ataatgtaag
    12721 ggtatttcca ttatcggaaa agattatttc ataaaggctt acattgtgaa aaatattttt
    12781 tttcatttga caaaatatcc agaatgatga tgattatttg gcaatggatt gccttcataa
    12841 tcttgtcgtt gcggatattt tccctgtaca ccatcggttc gcggcactct ttgggttctt
    12901 ataacctctc catttggacg aatatctatt ttcacgccat caggaccttt ccatgtttca
    12961 tattgagccg catctgaacc agtacgtcta aaaccttgtt cttgcaatgc ttgacgtaat
    13021 tcatctcgcg tagttggtct gatagattta acaactggct tgtttcctcc tgccatctca
    13081 ccgtccaacg tctgcatagg gcgggcagca ggtgttttat agccgttatt tttaacccaa
    13141 tcctatttgg tcgggttaag ttctactttt ttacctctcc aaaccgtacc tgcggcctct
    13201 gctacggcaa gggcagtttg ggctgttgca gttatattcg gatgggcatc agcccattgt
    13261 ctggcggaat tgataccgtc ttttaccgca aaagtactgt cctataatgc gcttgcggca
    13321 gcaagttgtg ctttcggact taaatttcct aaatcattaa taccttgtaa agtttgctgc
    13381 gcagagatgt aggtcggata tttgtatccg acaaaaatat ttgacgcatc tatcgtttcc
    13441 gaaatgccgc tgttggaaaa tgtcggattc gagaatccga cctacggcta tttcgtttaa
    13501 gtcataaata gccattcatg ttccttctga atttgctttt gctatcctta gaaccaatca
    13561 tataaacatt atcatcatta attaatgttc taagtttatg aagcggtcaa atctgtcaaa
    13621 ctaccagaga tgaagcatat cctcctggaa gtaattctgt tcgtgctgtt tccatatctt
    13681 ctttgccatc atcagttact tctctgtcta aataaaatct tacgataagt ttttgtctaa
    13741 tttcatcaaa ttctgccgta attaatctta tattgatatg aatacacggt tcatacctgc
    13801 tattagccag tcaggtgtag ttggatgggg attcattttt aataatatct tcaagcaagt
    13861 gaaatgtaag aaaagcgtag atacttgaag gccggctatc tgattggcaa aaagaaattt
    13921 cggagataaa gaatttccgt ttatattttt cataaattaa atcaacacct tgttttacat
    13981 aaaaactaac ttcctcagga aggatattta taacattctc tccttttact tttatagtta
    14041 atggtttgat ttttatgttt tcatgaatat ctgataggat tatttctaaa aaattatttt
    14101 tatttccagt cattctggct attttatgta tatttccatt gtgttcgatt atcattttat
    14161 tttttccaag gatttccttc tatattgata gtttatctaa agatttcatt taaaatatcc
    14221 tgactaattt cttgtttata taattctaca attttctgtt tctgtgtatt tgtataaaat
    14281 ttttcgttgg tatctgcttg atattttaat tgtctcaata tttccatgat ttcatattct
    14341 gatttttcat catattcttc aatcatgtaa tcaacaaatt gttttagaat atcaaaggct
    14401 tcgtaacttt ccaaaatttt aatatgttta tcggcaaaat tggacatata ccattcgtca
    14461 gtgattggat tagtatttaa atcatctaaa aaaacattta aatcatctaa aaaaacattt
    14521 acatcatcta aaaacatgac taatccttaa acttaaaatc tgttttctca ccggttcttg
    14581 agttttcaat gtaatgtatt tcaattttgt ttacgttttg aacacgctta acccaaccgt
    14641 cttttgctaa ccaaccattt ttagtatcgg acataggagg tattctcgca ggagttttgc
    14701 cctgtgggtt tgccttaact tcttccattg ctaattgttc ctttaaatta ttaggtattg
    14761 tacgaccggt tgatttttca acagattgtg tccctatttt taatgatttg tttcccccag
    14821 ccatttcccc gtctacagtc tgcatagggc gggcagcagg ttttttatag ccggtatttt
    14881 taacccaatc ccatttagtc gggttaagtt ctactttttt accgcgccaa accgtacctg
    14941 cggcctctgc tacggaaagg gcagtttggg ctgttgcagt tatattcgga tgggcatcag
    15001 cccattgtct ggcggaatta atgccgtttt ttaccgcaaa agcactgtcc tgtaataggc
    15061 tcgcggcagc aagttgtgct tccggactta aatttcctaa atcattaata ccttgtagag
    15121 tctgctgagc ggctgtatct gagccggtca gtgtaggttg gattaaaacc cagcattttc
    15181 aacatcttga tgttttacta taaatctatt cttgtgcttc ctttgttaga taaatttcaa
    15241 cacaaaagat acgtatgcat tctctaattt tatttgagat aaataatgca ttgctttgtc
    15301 gatactgtta aataaattac ttccctcgta ataccaatta tcatatgttt gataaaaata
    15361 attatctttc ttgatataaa tatctccccc caatacaagt attctctcat tgataaataa
    15421 atttaatata tctattgcaa tagggacggg taatgcggct tctctaattc caattcctat
    15481 caaagattcc ccttgtctcg aataaatttc aaaaatttca tttaacatgt taatctctat
    15541 ctactatttt tcaatcggta taaaccctat tcacagtttc atcttgtatt tctttatgaa
    15601 tacttaatat attaagatag cttaggaatt ttattttctc agattgaggc aactcatcaa
    15661 aaccaagcca acaataaaaa ttgttatatg attttaaaaa atcaacaatt tttatttttc
    15721 ctaaaaccaa atcttcgact aactgggttg cataaataat atcatttatg attttaacag
    15781 gtaaattatt tgataggtaa ccatgggatt ccgtcatcat tgatttctct aggtgcttat
    15841 ttatctgatt ggtaagcctt ttgcgtaagc ttctttcgcc tccgcaagtg tcatttcatt
    15901 agggccacca tctatattag tttctcctgt attttgccac tgacaaacgt cacagatatc
    15961 atagtccata acttcagttc cgcaaacagg acaatgtagc catatttctc cgtcaatttc
    16021 ccatgtcttt tttgaattct ccatcataat acttctttcc taagtttggt ttaaacattg
    16081 ttttgatttt gaatgttttt ggattggcca aaacatagat attgttattg atatcatagc
    16141 gcactcgtct atgttccgtc tgataaccta atatcttgtc cgatgtctgt tttgataaca
    16201 aatcagacgc cattttttgg tagtcttcaa tggttatatc gccaaattct tttccatgag
    16261 ctttgaaatg tccatttaaa gatttctcag taggaaactt ggactttgtc catctgatgc
    16321 ggtcttgaat tgttcctgtc ggagaaatag atgtatcagg tttaacatat gaggcaggta
    16381 agccgatatt gttatcagag tgtgtagggt gttgctgcgt acttggtttt ggtggcttat
    16441 tcccacctgc catctcccca tctaaagtct gcatagggcg ggcagcaggt gttttatagc
    16501 cggtattttt aacccaatcc catttggtcg ggttaaggtt tactttttta ccgccccaaa
    16561 ccgtagttgc ggcttctgct acggaaaggg cagtttgggc tgttgcagtt atattcggat
    16621 gggcatcagc ccattgtctg gcggaattaa tgccgtcttt taccgcaaaa gcactgtcct
    16681 gtaataggct cgcggcagca agttgtgctt ccggacttaa atttcctaat tcattaatat
    16741 cttgtaaagt ttgctgcgca gagatgtagg tcggatattt gtatccgaca aaaatatttg
    16801 acgcgtctat cgtttccgaa atgccgctgt tgaaaaacgt cggattcgag aatccgacct
    16861 acggctgcta ccaaccatca ttttcagctt cttttagcca agtatcaaaa ttttcaaaag
    16921 tctcgtaata ttttgtatta tctaaattaa ctactataat tttaggatta cctgatttat
    16981 ctgaaatatc aaaacaggca gcccactctt ggtttctagc aaaaggtaat aggtttggaa
    17041 tgccagttat ttcaaaataa atgtcaatta cttctgatat attgctttct gcattttcaa
    17101 accaccaagg aaaagaatat tgttcatcgt agttaatggc atgagtggat tgagctaatt
    17161 ttaaataact ttgcggatat ttaaatcctt taggaagcat tgcttcagga tacggaacaa
    17221 aactattttg ttttatcatt tcctaagtac ctttacatca taaatacttc cgttaggaaa
    17281 tcttttgctc cacccttctg gaactccttt ttgtgtttct tgctttctaa ttcgtagctg
    17341 gatagcttct ccccaagttt gaggctcaat accatcatgc ttaaacctga ttctctcttt
    17401 tgatgataat cggtcattaa aattatgaac attcgcttcc acttttaccc ctgcttcacg
    17461 tgctgccata atacgcgtgt tatccatact agtaggtgct ccatcaggca tattaacaac
    17521 atcaaccctg tcgccaaccc atgatttttt tctttcattt cggctacaat agaatcatag
    17581 ttgtaatttg cactaggctc tgacaaaaat ttgggttgtt aagttgtcat ttttttataa
    17641 attccagcaa actgttgttg taaattatct acttcatgtt ttctaacacc agatacattt
    17701 tcaaattctc ttgaatctac acatacgccc gcatatatct cattcaagat agctcgtaaa
    17761 acaaaaactt catcttgagt tagtgaaata gttgagtttt gattatttat ggatagtata
    17821 ttcatggctt tttcctataa atcatcataa tattttttac ctgatgttgg tctaaatgct
    17881 gtacctccat cgtcagaatt tttatctcgg ataactattg tccctgtttt atcatcccaa
    17941 tacgcagttc taccgcgagg taactctttc atatttgttg gatggctaac aatattttca
    18001 atatgccgag caaaatctgc tggtgaattg atatttaaat ccgtaaattc ttgttgtctt
    18061 atgacatgct tgttataagc atgcccacta ctaatttgtt ctccaattag ttgcgcttgt
    18121 aaagacggtt gtgtggaagc atctgctttg ctgttggacg ttatagattt aggcggtcta
    18181 ttcccccctg ccatctcccc atctacagtc tgcatatggc gggcagcagg ttttttatag
    18241 ccggtatttt taacccaatc ccatttggtc gggttaagtt ctactttttt accgc
//
LOCUS       DAWHSK010000013        18108 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.013, whole genome shotgun sequence.
ACCESSION   DAWHSK010000013 DAWHSK010000000
VERSION     DAWHSK010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18108
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.013"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..450
                     /locus_tag="WIC03_000343"
     CDS             <1..450
                     /locus_tag="WIC03_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEZ3882899.1"
                     /translation="SSTSVYGDTARECDETATPDPQTESARQILAAEQHLLDSGISNI
                     DILRLGGLYCAERHPVSRLVQKQNIPGGNRPINIVHRNIAVESLFQTAFNPGGRRLKN
                     IIEPRHPTRREFYTEEAAKLGLPAPDFAPDDSVGKIIRTVCDNGLSL"
     gene            501..1067
                     /locus_tag="WIC03_000344"
     CDS             501..1067
                     /locus_tag="WIC03_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HEZ3882900.1"
                     /translation="MSAFLPIINRLILQSPDSRSELAAFAGKTLTLNIAGLKLAGRIT
                     EDGLLSAGNGFADTEITFRNSAVQKILQGGEPGAGDIGLEGDLILGIAVLSLLGSLRS
                     RASDELARIFGTQADIGSRAADIGHGIKQIGRNIAEQIGGFSRESESANIGNEALADC
                     LDEISRLRDGVVRLNERLDRLERDIWID"
     gene            1087..1977
                     /locus_tag="WIC03_000345"
     CDS             1087..1977
                     /locus_tag="WIC03_000345"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HEZ3882901.1"
                     /translation="MNSPFHNIGIVTRPNTPDIQDTAHTLITFLKQHGFTVYLDEVGI
                     KEGCIYTQDTVGCHIVNKTELGQYCDLVAVLGGDGTFLSVAREIALRAVPIIGINQGH
                     LGFLTQIPREYMTDKLLPVLEGKYLAEERILIEAALIREGKTAERAIALNDAVLSRGG
                     AGQMIEFEVFVNREFVYTQRSDGLIVSTPTGSTAYSLAAGGPIMQAGLHAFTLVPICP
                     QSMTNRPIAIPDTSEIEILVTQGGDARVHFDGQTHIDVQNLDRITIRRYRNPLRILHP
                     TDYQYFKTLRQKLHWGEQLV"
     gene            2002..2760
                     /locus_tag="WIC03_000346"
     CDS             2002..2760
                     /locus_tag="WIC03_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEZ3882902.1"
                     /translation="MNPKTPPDHTEDAVRLSKRMAQLGLCSRREADGYIEQGWVTVNG
                     KTAVLGQKVSPADRIELNKKAHEQQAARITILLNKPVGYVSAQAEKGYKSAAELITPE
                     NRWEGDTGRIRFDPKHKIGLAPAGRLDIDSVGLLVLTQDGRIAKQLIGENSGSEKEYL
                     VRVRGKLDEKGLALLNHGLSLDGEKLRPAQVEWQNEDQLRFVLKQGKKRQIRRMCELV
                     GLRVVGLKRIRMGKVKLGRLPPGKWRYLAPGESF"
     gene            complement(2815..3480)
                     /locus_tag="WIC03_000347"
     CDS             complement(2815..3480)
                     /locus_tag="WIC03_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ3882903.1"
                     /translation="MAVLSKEQVLSAFKNRKSCRHYDAARKISAEDFQFILELGRLSP
                     SSVGSEPWQFVVVQNPEIRQAIKPFSWGMADALDTASHLVVFLAKKNARFDSPFMLES
                     LKRRGVTEPDAMAKSLARYQAFQADDIKILDDSRALFDWCCRQTYIALGNMMTGAAMA
                     GIDSCPVEGFNYADMERVLSGQFGLFDAAEWGVSVAATFGYRVQEIATKARRPLEETV
                     IWA"
     gene            complement(3680..4576)
                     /locus_tag="WIC03_000348"
     CDS             complement(3680..4576)
                     /locus_tag="WIC03_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE2"
                     /protein_id="HEZ3882904.1"
                     /translation="MQLLKASDIISHLQIDGIFPGGNAIPCTHLNNYMNIKEKQLMNT
                     IADVQSSRDLRNLPINQVGIKDLRFPITLQTAEGIQSTIARLTMTVYLPAEQKGTHMS
                     RFVALMEQHAEALDFAQLRKLTTEMVALLDSRAGKISVSFPFFRKKTAPVSGIRSLLD
                     YDVCLTGEIKDGAYGHSMKVMIPVTSLCPCSKEISQYGAHNQRSHVTVSLTADAEVGI
                     EEVIDYVEAQASCQLYGLLKRPDEKYVTEKAYENPKFVEDMVRDVATSLIADKRIKSF
                     VVESENFESIHNHSAYAYIAYP"
     gene            4645..5802
                     /locus_tag="WIC03_000349"
     CDS             4645..5802
                     /locus_tag="WIC03_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEZ3882905.1"
                     /translation="MKFATKAIHSSYDCDEHNRALMPPIYQNSMFALHEIGENVPYRY
                     SRLSNPTRQILEDTVADLEHGAAGFAFSSGMAGIDAVWRTFLRPGDTIVAVADIYGGA
                     YDLLVDVYQKWGVNVVFADLGNPDNLDELLKAHKVKLVWLETPSNPLLRLVDIKVLAA
                     KAKAAGALVGIDNTFATPYLQQPLDMGCDFVFHSATKYLCGHSDVLMGIVVAKTKELA
                     QPLHDMMVHTGAVAGPLDCWLVLRGIKTLALRMNAHCQNALEIARRLEAHPAIEKVFH
                     PGLPSHEHYELAKTQMPKGIGGVVTVYLKNDTREAANSVIKNMKLVKMASSLGGVESL
                     VNHCYSQSHSGVPHDVKMEMGIKVGLLRFSIGIEDADDIWNDISAALDTTL"
     gene            complement(5865..6866)
                     /gene="hemB"
                     /locus_tag="WIC03_000350"
     CDS             complement(5865..6866)
                     /gene="hemB"
                     /locus_tag="WIC03_000350"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEZ3882906.1"
                     /translation="MQFPYRNVPASRMRRMRRDDFSRRLMREHTLTADDLIYPVFVLE
                     GSAREEDVPSMPGVKRQSLDRLLFTAEEAVKLGIPMLALFPVVTANKTERAQEAYNPE
                     GLVPSTVRALRERFPELGIMTDVALDPYTVHGQDGLTDENGYVMNDETVEVLVKQALC
                     HAEAGAQVVAPSDMMDGRIGAIREALEDAGHIHTRIMAYSAKYASAFYGPFRDAVGSS
                     GNLGKADKKTYQMDPANTDEALNEVALDIREGADMVMVKPGLPYLDVVRRVKDEFGVP
                     TYAYQVSGEYAMLQAAIANGWLDGGKVVLESLLAFKRAGADGILTYYAIEAAKMLKR"
     gene            complement(7049..7333)
                     /gene="yhbY"
                     /locus_tag="WIC03_000351"
     CDS             complement(7049..7333)
                     /gene="yhbY"
                     /locus_tag="WIC03_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEZ3882907.1"
                     /translation="MTDTKLNTKEILELKARAHHLHPVVMVGQQGLTDAVIKETDAAL
                     TAHELIKVRVFGDDRAERIEICTALCEAVDAQPVQHIGKLLVLWRKNIEG"
     gene            7443..8063
                     /locus_tag="WIC03_000352"
     CDS             7443..8063
                     /locus_tag="WIC03_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HEZ3882908.1"
                     /translation="MAVRSKSSKAWLHEHVNDHYVHMAQKDGYRARAAYKLLEINEKD
                     KLIKPGTVLADLGSAPGSWSQVAAKLTGTSGAVFALDILPMEAIGGVSFIQGDFREND
                     VLAQFETLLDNRPLDLVICDMAPNMSGNAVSDQARSFYLCELALDFASQHLKTGGSFL
                     VKVFQGAGYQEYMAAMREIFGTVQTRKPEASRNRSSEIYLLGKNKR"
     gene            8127..10094
                     /gene="ftsH"
                     /locus_tag="WIC03_000353"
     CDS             8127..10094
                     /gene="ftsH"
                     /locus_tag="WIC03_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEZ3882909.1"
                     /translation="MGNTFKSILVWVALGIGLMAAFNALDGKKEDNGQIEYSQFIQQV
                     NNGEVSGVNIEGSVVSGYLIKGERTDKSTFFTNAPLDDNLIKTLLDKNVRVKVTPEEK
                     PSALAALFYSLLPVLLLIGAWFYFMRMQTGGGGKGGAFSFGKSRARLLDKDANKVTFA
                     DVAGCDEAKEEVQEIVDYLKAPNRYQSLGGRVPRGILLAGSPGTGKTLLAKAIAGEAG
                     VPFFSISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIIFIDEIDAVGRQRGAGLGGGN
                     DEREQTLNQLLVEMDGFESNQTVIVIAATNRPDVLDPALQRPGRFDRQVVVPLPDIRG
                     REQILNVHSKKVPLDKSVDLLSLARGTPGFSGADLANLVNEAALFAGRRNKVKVDQSD
                     FEDAKDKIYMGPERRSMVMHEDEKRATAYHESGHAIVAESLPFTDPVHKVTIMPRGRA
                     LGLTWQLPERDRISMYKDQMLSQLSILFGGRIAEDIFVGRISTGASNDFERATQMARE
                     MVTRYGMSDKMGVMVYAENEGEVFLGRSVTRSQNISEKTQQDIDAEIRRILDEQYQVA
                     YKILDENRDKMETMCKALMEWETIDRDQVLEIMAGKQPSPPKDYSHNLRENADAAEDN
                     APHAPTREETEAPAPADTASTESEQQPENKA"
     gene            10316..10747
                     /locus_tag="WIC03_000354"
     CDS             10316..10747
                     /locus_tag="WIC03_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HEZ3882910.1"
                     /translation="MKKVEKNILVLHGADKMFELVDKVEDYPNFLPWYSKTEVIGRNG
                     NELKARLFMDYMHVRQSFATHNRNIPGREIRMELLEGPFKTLRGTWKFIDLGDDMCKI
                     EFNLEYDFSNAVLSALISPVFNHLSATLVEAFVKEADRRYA"
     gene            10740..11018
                     /locus_tag="WIC03_000355"
     CDS             10740..11018
                     /locus_tag="WIC03_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HEZ3882911.1"
                     /translation="MLEIEIVYGLPDRQVLKTMQLAEGTTVRAAALQSGLDGIFEDLN
                     LHSAPLGIFGKAVKDDTPLRDGDRIEVYRPLLIDPKEARRKRVQNQEE"
     gene            11071..11649
                     /gene="pth"
                     /locus_tag="WIC03_000356"
     CDS             11071..11649
                     /gene="pth"
                     /locus_tag="WIC03_000356"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEZ3882912.1"
                     /translation="MSNKIKMLVGLGNPGKEYEQTRHNAGFWFLDELAWKWKASFKEE
                     KKFFGEVARAALPDGDVWLLKPATFMNRSGQAVAALAQFYKIKPEEILVVHDELDIPC
                     GRIKFKLGGGNGGHNGLKDIQAKLGTADYYRLRLGIDHPGDRNLVVGYVLNKPSTEHR
                     RQIDDAVAKSLQAIPDILAGKWEEATRFLHSK"
     gene            11710..11916
                     /locus_tag="WIC03_000357"
     CDS             11710..11916
                     /locus_tag="WIC03_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HEZ3882913.1"
                     /translation="MTELKQLIQTESIPVIEETLDFLLYECSIDDAPSAEEVAQWRDI
                     LAARGGKFLRLSKICQTWLDEEAA"
     gene            11913..12275
                     /locus_tag="WIC03_000358"
     CDS             11913..12275
                     /locus_tag="WIC03_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEZ3882914.1"
                     /translation="MKLPRNRFSLLSALWFAGGIYSLLFKAADTAPPPFPHFDKAAHL
                     ALFFAQIWLLTKAFKTGKLPIPYRSLMVFALCFALFSECAQAWFTATRTGSLGDVLAD
                     MAGTVLALFAARAADRPD"
     gene            12506..13921
                     /locus_tag="WIC03_000359"
     CDS             12506..13921
                     /locus_tag="WIC03_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEZ3882915.1"
                     /translation="MNLHAKDKTQHPENVELLSAQKPITDFKGLLTTIISAVVCFGIY
                     HILPYSPDANKGIALLIFVAALWFTEAVHITVTALMVPILAVVLGFPDMDIKKAMADF
                     SNPIIYIFFGGFALATALHMQRLDRKIAVSLLRLSRGNMKVAVLMLFLVTAFLSMWIS
                     NTATAAMMLPLAMGMLSHLDQEKEHKTYVFLLLGIAYCASIGGLGTLVGSPPNLIAAK
                     ALNLDFVGWMKLGLPMMLLILPLMLLSLYVILKPNLNERVEIKAESIPWTLHRVIALL
                     IFLATAAAWIFSSKIKTAFGISNPDTVIALSAAVAVVVFGVAQWKEVARNTDWGVLML
                     FGGGISLSTLLKTSGASEALGQQVAATFSGAPAFLVILIVAAFIIFLTEFTSNTASAA
                     LLVPIFSGIAMQMGLPEQVLVFVIGIGASCAFMLPVATPPNAIVFGTGLIKQREMMNV
                     GILLNILCVVLVALWAYAVLM"
     gene            14040..14549
                     /locus_tag="WIC03_000360"
     CDS             14040..14549
                     /locus_tag="WIC03_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEZ3882916.1"
                     /translation="MIILHTNKGDIKIELDFDKAPVTAKNFEQYVKDGFYDGVIFHRV
                     IKGFMIQGGGMDENMNEKETRDPIQNEASNGLPNDKYTIAMARTSDPHSASAQFFINT
                     ADNAFLNFRSKELYGKTVVQDWGYAVFGKVVDGFDVVDAIEGVSTKRHGYHDDVPSEP
                     VVIIKAEAV"
     gene            14712..16094
                     /locus_tag="WIC03_000361"
     CDS             14712..16094
                     /locus_tag="WIC03_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEZ3882917.1"
                     /translation="MANIARDIFKAYDIRGIVGKTLTDDAAYLIGRAIAAKAAEKGMT
                     RIALGRDGRLSGPELMEHIQRGFTDSGIDVLNVGMVATPMLYFAAINECGGSGVMITG
                     SHNPPDYNGFKMMLGGDTLAGEAIQELLAIIEKDGFVAADKQGNVTEKDISGEYHNHI
                     VGHIKLKRPMNIAIDAGNGVGGAFAGKLYKGLGNEVTELFCDVDGNFPNHHPDPSKPK
                     NLQDLIAALKNGDAEIGLAFDGDADRLGVVTKDGNIIYPDRQLMLFAQDVLSRNPKAK
                     VIFDVKSTRLLAPWIKEHGGEPVMEKTGHSFIKSSMKKTGAPVAGEMSGHIFFKERWF
                     GFDDGLYAGARLLEILSASDNPSEVLNNLPQSISTPELNIALPEGSNGHQVIDELAAK
                     AEFEGATEIITIDGLRVEFPDGFGLMRASNTTPILVLRFEADTQEAIERIQNQFKAVI
                     ESNPNLIWPL"
     gene            complement(16114..16869)
                     /locus_tag="WIC03_000362"
     CDS             complement(16114..16869)
                     /locus_tag="WIC03_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEZ3882918.1"
                     /translation="MIKIRNIHKTFGENTILRGIDLDVGKGQVVVILGPSGSGKTTFL
                     RCLNALEMPEDGQIEFDNERPLKIDFSKKPSKHDILALRRKSGMVFQQYNLFPHKTAL
                     ENVMEGPVAVQGKPAAQAREEALKLLEKVGLGDKVDLYPYQLSGGQQQRVGIARALAI
                     QPELMLFDEPTSALDPELVQDVLNAMKELAREGWTMVVVTHEIKFALEVATTVVVMDG
                     GVIVEQGSPKELFDHPKHERTRRFLSQIQSTKI"
     gene            complement(16879..17595)
                     /locus_tag="WIC03_000363"
     CDS             complement(16879..17595)
                     /locus_tag="WIC03_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEZ3882919.1"
                     /translation="MFNNFLASLPFMTETRADMIVSAFLPMVKAGFAVSLPLAAASFV
                     IGMMIAVAVALVRIMPAGGIVRKILLKLVEFYISVIRGTPLLVQLVIVFYGLPSVGIY
                     IDPIPAAIIGFSLNVGAYASETIRAAILSVPKGQWEAGFSIGMTYMQTFRRIVAPQAF
                     RVAVPPLSNEFIGLFKNTSLAAVVTVTELFRVAQETANRTYDFLPVYIEAALVYWCFC
                     KVLFLIQARLEKRFDRYVAK"
     gene            complement(17585..>18108)
                     /locus_tag="WIC03_000364"
     CDS             complement(17585..>18108)
                     /locus_tag="WIC03_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEZ3882920.1"
                     /translation="GLKAGRFDVVANQVGLTSPERQATFDKSDPYSWSGAVLVARNDS
                     NIKSIDDIKGVKTAQSLTSNYGEKAKAAGADLVAVDGLAQSLTLIEQKRADATLNDEL
                     AVLDYLKKNPNAGVKIVWSAPADEKVGSGLIVNKGNDEAVAKFSTAINELKADGTLKK
                     LGEQFFGKDISVQ"
BASE COUNT         4497 a         5312 c         4448 g         3851 t
ORIGIN      
        1 agcagcacca gcgtttacgg cgatacagcg cgcgaatgcg acgaaaccgc cacacccgat
       61 ccgcaaaccg agtccgcccg ccaaatcctc gccgccgaac aacacctgct cgacagcggc
      121 atctccaaca tcgacatcct gcggctgggc gggctttatt gcgccgaacg ccatcccgtc
      181 agccgccttg ttcaaaagca aaacatcccg ggcggcaacc gccccatcaa catcgtccac
      241 cgtaatatcg ccgtcgaaag cctgtttcag acggcattta accccggcgg caggcggctg
      301 aaaaacatta tcgaaccgcg ccacccgaca cgacgcgaat tctacacgga agaagccgcc
      361 aaactcggcc tgcccgcgcc ggatttcgca cccgacgaca gcgtgggcaa aatcatccgt
      421 accgtttgcg ataacggctt aagcctgtaa aataagcggc aacagcaaca aacaggcatt
      481 ctccgcccca atcagaaaat atgtccgcct tcctccccat catcaaccgc ctgattctgc
      541 aaagcccgga cagccgctcg gaacttgccg cctttgcagg caaaacactg accctgaaca
      601 ttgccgggct gaaactggcg ggacgcatca cggaagacgg tttgctctcg gcgggaaacg
      661 gctttgcaga caccgaaatc accttccgca acagcgcggt acagaaaatc ctccaaggcg
      721 gcgaacccgg ggcgggcgac atcgggctcg aaggcgacct catcctcggc atcgcggtac
      781 tgtccctgct cggcagcctg cgttcccgcg catcggacga attggcacgg attttcggca
      841 cgcaggcaga catcggcagc cgtgccgccg acatcggaca cggcatcaaa caaatcggca
      901 ggaacatcgc cgaacaaatc ggcggatttt cccgcgaatc cgagtccgca aacatcggca
      961 acgaagccct tgccgactgc ctcgacgaaa taagcagact gcgcgacggc gtggtacgcc
     1021 tcaacgaacg cctcgaccgg ctcgaacgcg atatttggat agactaacct tcagacggca
     1081 tccgacatga acagcccttt tcacaacatc ggcatcgtaa cccgccccaa cacgcccgac
     1141 atccaagaca ccgcgcacac gctgattacc tttttgaagc agcacggctt taccgtctat
     1201 ctcgacgaag tcggcataaa ggaaggctgc atctataccc aagacaccgt cggctgccat
     1261 atcgtcaaca agaccgaact ggggcaatac tgcgacctgg tcgccgtttt aggcggagac
     1321 ggcacctttc tctccgtcgc ccgcgaaatc gccctgcgcg ccgttccgat tatcggcatc
     1381 aaccaagggc atttgggctt cctgacccaa attccccgcg aatatatgac ggacaagcta
     1441 ttgcccgttt tagaagggaa ataccttgcc gaagagcgca tcctgattga ggccgcactc
     1501 atccgcgaag gcaaaaccgc cgaacgcgcc atcgccctca acgatgccgt cctctcccgt
     1561 ggcggcgccg gacagatgat tgagtttgaa gtcttcgtca atcgggaatt cgtctatacc
     1621 cagcgttcgg acgggctgat tgtctccacc cccaccggat cgaccgccta ttcgcttgcc
     1681 gccggcggcc ccatcatgca ggcaggattg cacgccttca cgctcgtccc catctgcccg
     1741 caatccatga ccaaccgccc catcgccatt ccagacacgt ccgaaatcga aatcctcgtt
     1801 acccaaggcg gcgacgcgcg cgtccatttc gacggtcaaa cccatatcga cgtgcaaaac
     1861 ctcgaccgca tcaccatccg ccgctaccga aaccccttac gcatcctaca tccgaccgac
     1921 taccaatatt tcaaaaccct gcgccaaaaa ctgcactggg gcgaacaatt ggtctgaacc
     1981 gacccttacc ggaaaacacc tatgaatcca aaaactcccc ccgaccatac agaagatgcc
     2041 gtgcgcctct ccaaacgcat ggcgcaactg gggctttgtt cgcgccgcga agccgacggc
     2101 tacatcgaac aaggctgggt aacggtcaac ggcaaaaccg ccgtactcgg tcagaaagtt
     2161 tcaccggccg accgtatcga actaaacaag aaagcccacg aacagcaggc ggcacgcatt
     2221 accatcctgc tgaacaaacc tgtcggctat gtcagcgcac aagcggaaaa aggctataaa
     2281 tccgccgccg aactgattac ccctgaaaac cgctgggaag gcgacaccgg acgcatccgt
     2341 ttcgatccga aacacaaaat cggcctcgcc cccgccggca ggctggacat cgactcggtc
     2401 ggattgctgg tattgacgca ggacggccgt atcgccaagc agcttatcgg cgaaaacagc
     2461 ggcagtgaaa aagaatattt ggtgcgcgtg cgcggcaaat tggacgaaaa aggacttgcc
     2521 ttactgaatc acggattgag tttggacggc gagaaactgc gtcccgccca agtagaatgg
     2581 caaaacgaag accaactgcg cttcgtgttg aaacagggta aaaagcggca aatccgccgt
     2641 atgtgcgaac tggtcggact gcgcgtcgtc gggctgaaac gcatccgcat gggcaaggtc
     2701 aaactcggca ggctgccgcc cggcaaatgg cgttatctcg cccccggtga atcgttttaa
     2761 ataagcatta aaaaatgccg tctgaaaatc cttgcgtttt cagacggcat tgccttatgc
     2821 ccaaataacg gtttcttcca agggcctgcg cgctttcgtg gcgatttcct gaacgcggta
     2881 accgaatgtc gcggcgacgg acacgcccca ttctgccgca tcgaacaaac cgaactgccc
     2941 ggacaatacg cgttccatat cggcatagtt gaaaccttcc accgggcagg aatcgatacc
     3001 tgccatcgcc gcacccgtca tcatgttgcc caacgcgata taggtctggc ggcaacacca
     3061 gtcaaacaag gcgcgcgaat cgtccaaaat cttgatgtcg tcggcttgaa acgcctgata
     3121 cctcgccaaa gattttgcca tggcatccgg ttcggtaacg ccgcgccgtt tgaggctttc
     3181 caacataaac gggctgtcga agcgggcatt tttctttgcc aaaaacacca ccaaatgact
     3241 ggcggtatcc aaagcatccg ccatacccca agaaaacggc ttgattgcct gtcggatttc
     3301 ggggttttga accacaacaa actgccaagg ctccgaaccg accgaactgg gcgacaaacg
     3361 cccgagttct aaaataaact gaaaatcctc ggcactgatt ttacgtgccg catcgtaatg
     3421 ccggcatgat ttgcggtttt taaatgcgga tagaacctgc tctttgctta atactgccat
     3481 cgcacatcct ttcattactt gtattgcaaa ttgatttcaa accgatacgg cactgccgca
     3541 ccatacaatc cgtcctcgcc atcaagcttc atccggactt tacccatctc tctacggctg
     3601 ggaaaacaaa gcggcggcag actcgggaat aacgccccaa tgcctttcag acggcatttg
     3661 ggcatcattg caaacgcgcc tacgggtagg cgatataggc ataagccgaa tggttgtgga
     3721 tagactcgaa attctcgctc tcgacaacga aactcttgat gcgtttgtcg gcgatcagcg
     3781 aagtagcgac atcacgcacc atatcttcca cgaatttcgg gttttcgtag gctttttcgg
     3841 taacgtattt ttcatcgggg cgtttgagca aaccgtagag ttggcagctt gcctgcgcct
     3901 ccacataatc aatgacttcc tcgataccga cttctgcatc ggcagtcagg ctgacggtaa
     3961 catgcgaacg ctggttgtgc gcgccgtatt gggaaatttc tttggaacac gggcaaagcg
     4021 aggttacggg aatcatgacc ttcatactgt ggccgtatgc cccgtctttg atttcgcccg
     4081 tgagacagac atcataatcc agtaaggacc gaataccgga aaccggcgcg gttttcttgc
     4141 ggaaaaacgg aaaagaaacg ctgattttgc cggcgcggga atctaaaagc gcgaccattt
     4201 cggtagtcag cttgcgcaat tgtgcaaaat ccaaggcttc ggcatgttgc tccatcaatg
     4261 cgacaaaacg cgacatatgc gtccccttct gctcagcagg caggtaaacc gtcatggtca
     4321 gacgggcaat ggtggactgg atgccttctg cagtttgcag ggtaatcgga aagcgcaggt
     4381 ctttgatacc gacctgattg atcggcagat tgcgcaaatc tcggctggat tgcacgtctg
     4441 caatagtgtt catgagttgt ttttccttaa tattcatgta attgttgaga tgtgtgcatg
     4501 gtatggcgtt tccgccgggg aatataccgt caatctgcaa atgcgagatt atatcactcg
     4561 cttttagcag ctgcattgat tgtttctatt tttcagatag tgataaaatc ttcgattatt
     4621 cacatctaca taggcagaga ccaaatgaaa ttcgccacga aagccattca ttccagctac
     4681 gattgcgacg aacacaaccg cgcgctgatg ccgccgattt atcaaaacag tatgtttgcg
     4741 ttgcacgaga ttggcgaaaa tgtgccttac cgttattcgc gcctgagcaa cccgacccgt
     4801 cagattttag aagacaccgt tgccgatttg gaacacggtg cggcaggttt tgcgttttcc
     4861 agcggtatgg cgggaattga tgccgtatgg cgcactttcc tgcgcccggg cgataccatt
     4921 gtcgccgtcg ccgatattta cggcggcgct tatgatttat tggtcgatgt ttatcaaaaa
     4981 tggggggtga acgttgtttt tgccgattta ggcaatccgg ataatttgga cgaactgctt
     5041 aaagcgcaca aggtcaaact ggtttggctg gaaacgccgt ccaatccact tttacgcttg
     5101 gtagacatca aagtccttgc cgcaaaagcc aaagcagccg gtgcgctggt cggtatcgac
     5161 aacacttttg ccacgccgta tctgcaacag ccgttggata tgggttgcga ttttgtattc
     5221 cattccgcta ccaaatattt gtgcggccat tccgacgtgt tgatgggcat cgtcgttgcc
     5281 aaaaccaaag aactggcgca gcctttgcac gatatgatgg tgcataccgg cgcggttgcc
     5341 ggcccgctgg actgctggct ggtgttgcgc ggcatcaaaa cactggctct gcgcatgaac
     5401 gcccattgcc aaaacgcact cgaaatcgcg cgccgtttgg aagcccatcc tgccattgaa
     5461 aaagtgttcc atcccggcct gccgtctcac gaacattacg aactggcgaa aacacaaatg
     5521 cccaaaggca tcggcggcgt ggttacggtt tatctcaaaa acgacacgcg tgaagcggca
     5581 aacagcgtga ttaaaaacat gaaactggtc aaaatggctt ccagcctcgg cggtgtggaa
     5641 agtttggtca accattgcta ttcccagtcc cacagcggcg taccgcatga tgtgaaaatg
     5701 gaaatgggca tcaaagtcgg tctgctgcgt ttctccatcg gcatcgaaga cgcggacgat
     5761 atttggaacg atatttccgc cgcactcgat acgactttgt aaactgtaaa aatgccgtct
     5821 gaaaccatgg tttcagacgg catttcaatt aacccggccg aaaatcaacg cttcaacatc
     5881 tttgccgcct caatagcgta ataggtcaaa atcccgtccg cacccgcacg tttgaatgcc
     5941 agcaggcttt ccaaaaccac tttgccgccg tccagccagc cgttggcaat cgctgcctgc
     6001 aacatcgcgt attctcccga aacctgatag gcataagtcg gtacgccgaa ctcgtccttc
     6061 acgcggcgga caacgtccaa atacggtaaa ccgggcttca ccatcaccat atccgcacct
     6121 tcccgaatat cgagcgccac ttcattcagc gcctcatcgg tatttgccgg atccatctgg
     6181 taggttttct tgtccgcctt gcccaaattg cccgaactgc ccaccgcatc gcggaacggc
     6241 ccgtaaaacg ccgaagcata tttggcggaa tacgccataa tccgcgtgtg gatatgcccg
     6301 gcatcctcca acgcctcgcg aatcgcaccg atacgcccgt ccatcatatc ggaaggggca
     6361 accacctgcg cgcccgcttc agcgtggcac aaagcctgct tgaccaaaac ctctacggtt
     6421 tcatcgttca tcacataacc gttttcgtcc gtcagcccgt cctgaccgtg aaccgtataa
     6481 ggatcgagcg cgacatccgt cataatgccc agttcgggaa acctctcgcg caaggcgcgg
     6541 acagttgacg gcacgagtcc ttcgggattg tacgcctcct gcgcacgctc ggttttgttt
     6601 gccgtaacca cggggaacag tgccaacatc ggaataccga gctttaccgc ctcttccgcc
     6661 gtaaacagca gcctgtccaa actttgacgc ttcacacccg gcatagaagg cacatcctcc
     6721 tcgcgcgccg acccctccaa tacgaacacc ggataaatca aatcatcggc ggtcagcgtg
     6781 tgttcgcgca tcaggcggcg tgaaaaatcg tccctgcgca tacggcgcat acgcgaagcc
     6841 ggaacattgc ggtaaggaaa ctgcataagc cctccaatca tcctgtcaac aattcaaacc
     6901 atacggaagc cgccgcaact tgcagacagc atcgggcggc aatcaaaatc cgttttcacc
     6961 ggaaagggtt tcggcccgcc gaaatgccgc gccgaaggac ggcgtcccgc ccattaaaga
     7021 acaatagcaa aacaacagct tcaggctgtc agccttcgat attcttacgc cacaatacca
     7081 aaagtttccc gatatgctga acaggttgcg cgtcaaccgc ctcacataag gcagtgcaga
     7141 tttcgatacg ttcggcacgg tcgtcgccga atacgcgcac tttaatcagc tcatgcgccg
     7201 tcaatgccgc atcggtttcc ttgatgaccg cgtccgtcag accctgctga ccgaccatca
     7261 caacaggatg gagatggtgc gcgcgcgctt tcagttccaa aatttctttg gtgttcaatt
     7321 tggtatccgt cattttccta ctgcttgaat aaaaagaata atgcggcatt gtacgcgatt
     7381 tggcacgaca tcggcaaaat taacgtacaa tacgcggttt ccattccgac ccgcgaacca
     7441 ctatggctgt acgttccaaa tcctcaaaag cgtggctgca cgaacacgtc aacgaccact
     7501 acgtccatat ggcgcaaaaa gacggctacc gcgcccgtgc cgcatacaaa cttttggaaa
     7561 tcaacgaaaa agacaaatta atcaaacccg gcacggtact tgccgacttg ggcagcgcac
     7621 cgggaagctg gtcgcaggtt gccgccaagc tgacgggtac ttccggagca gttttcgcct
     7681 tggacatcct gcctatggaa gccatagggg gcgtctcctt cattcagggc gacttccgcg
     7741 agaacgacgt actggcacaa ttcgaaacct tgttggacaa ccgcccgctc gaccttgtaa
     7801 tttgcgatat ggcgcccaat atgtcgggaa acgccgtaag cgatcaggca cgcagctttt
     7861 atctgtgcga actggcttta gacttcgcct cgcaacacct gaaaaccggc ggcagctttt
     7921 tggtcaaagt ctttcagggt gcaggctatc aggaatacat ggcagccatg cgcgaaattt
     7981 tcggcacggt gcagacgcgc aaacccgaag cctcgcgcaa tcgctccagt gagatttatt
     8041 tattgggcaa aaataaacgc tgacaataca gacggcgtgc tttacaatca tttccgtttt
     8101 acacctcaat tatggagcct tgctaagtgg ggaacacctt taaatcaatc cttgtctggg
     8161 tcgccttggg tatcggcctg atggctgcgt tcaacgcttt agacggtaaa aaagaagaca
     8221 acgggcaaat cgaatattct cagttcatcc aacaggtcaa caacggcgaa gtatccggcg
     8281 tcaacatcga aggatccgtc gtcagcggct acctgattaa gggcgagcgc accgacaaaa
     8341 gcaccttctt caccaacgcg cctttggacg acaacctgat taaaacactg ctcgacaaaa
     8401 acgtccgtgt aaaagtaacg ccggaagaaa aaccgagcgc gctggctgcc ctgttttaca
     8461 gcctgctgcc cgtcctgctg ctgattggcg cgtggttcta ctttatgcgt atgcagacgg
     8521 gcggcggcgg aaaaggcggc gcattctcat tcggcaaaag ccgcgcccgc ctactggaca
     8581 aagatgccaa caaagttacc tttgccgatg tcgccggctg cgacgaagcc aaagaagaag
     8641 tgcaggaaat cgtcgattac ctcaaagcgc cgaaccgcta tcaaagcctg ggcgggcgcg
     8701 tgccgcgcgg catcctgctg gcgggcagcc cgggtacggg taagacgctt ttggcgaaag
     8761 cgattgcagg cgaagccggc gtgccgttct tcagcatttc aggttccgac tttgtcgaaa
     8821 tgttcgtcgg tgtcggtgca agccgcgtcc gcgatatgtt cgagcaggcg aagaaaaacg
     8881 ccccctgcat catctttatc gacgagattg acgcagtcgg ccgccaacgc ggcgcaggtt
     8941 tgggcggcgg caatgatgag cgcgagcaaa cattaaacca attgttggtt gaaatggacg
     9001 gttttgagag caatcagact gtaattgtga ttgcggcaac caaccgcccc gacgtactcg
     9061 atcctgcgct gcaacgcccc ggccgtttcg accgccaagt ggttgtcccc ctgccggaca
     9121 tccgggggcg cgaacagatt ttgaacgtcc actctaaaaa agtgcctttg gacaaatctg
     9181 tggatttatt gtccctcgcg cgcggcacgc cgggtttttc cggcgcggat ttggcgaact
     9241 tggtcaacga agccgccctg tttgccggcc gccgcaataa agtcaaagtc gatcagagcg
     9301 attttgaaga cgccaaagac aaaatctata tgggtccgga acgccgcagt atggtgatgc
     9361 acgaagacga aaaacgtgcg acggcgtatc acgaatccgg acacgcgatt gttgccgaaa
     9421 gcctgccctt taccgacccc gtccacaaag taaccattat gccgcgcgga cgtgcgctgg
     9481 gtctgacttg gcagcttccg gagcgcgacc gcatcagtat gtataaagat cagatgttga
     9541 gccagctctc catcctgttc ggcggacgga ttgccgaaga catcttcgtc ggacgcatct
     9601 ccaccggcgc atcaaacgac tttgaacgcg caacccaaat ggcgcgcgaa atggtaacgc
     9661 gctacggcat gagcgacaaa atgggcgtga tggtttatgc ggaaaacgaa ggcgaagtct
     9721 tcttgggacg cagcgtaacc cgttcgcaaa acatttccga gaaaacccag caggacatcg
     9781 atgcggaaat ccgccggatt ttggacgagc agtatcaggt tgcctacaaa atcctcgatg
     9841 aaaaccgcga caagatggaa acgatgtgca aagccctgat ggaatgggaa accatcgacc
     9901 gcgatcaggt actggaaatc atggcgggca aacaacccag cccgcccaaa gattacagcc
     9961 acaacctgcg cgagaatgcg gacgcggcgg aagataacgc gccgcacgct ccgactcggg
    10021 aagaaaccga agcacctgcc ccggcagaca ccgcttcgac agagtccgag cagcagcctg
    10081 aaaacaaggc ttaacttccc gaacaaacgg cagcccgcaa gctgccgttt tcccatcctg
    10141 ttttcagacg gcattttccg ctcaatgccg tctgaaacgc caaagccgta tcggtttttg
    10201 cgaatctgcg atatggcggt ttaaattgaa atcagaacgg ggcaaggctg taccggttta
    10261 aaattaaacc gctataatac cgcccttcga atacacgccc gcattggtga tgcccgtgaa
    10321 aaaagttgaa aaaaacatct tggtgctgca cggcgcggac aaaatgttcg agctggtcga
    10381 taaggttgag gattacccga actttctgcc gtggtacagc aaaaccgagg tcatcggacg
    10441 caacggcaac gaactgaagg cgcggctgtt tatggactat atgcacgttc gccaatcatt
    10501 cgccacgcac aaccgcaaca ttccgggcag ggaaatccgt atggaactgc tcgaaggtcc
    10561 gttcaaaacc ttacgtggaa cgtggaaatt tatcgatttg ggcgacgata tgtgcaaaat
    10621 cgaattcaat ttggaatacg atttttccaa tgccgttttg tccgccttaa tttcccccgt
    10681 cttcaaccac ctttccgcca cgctggtcga agcgttcgtc aaagaggcag accgccgtta
    10741 tgcttgaaat tgagattgtg tacgggctgc ccgaccgaca ggttttgaaa accatgcagc
    10801 ttgccgaggg aacaaccgtc cgcgccgccg cactgcaaag cggtttggac ggcatatttg
    10861 aagatttaaa cctgcattcc gcgcctttgg gcattttcgg caaagccgtc aaagacgaca
    10921 cgcccctgcg cgacggcgac cgcatcgaag tgtaccgccc gctgttgatc gaccccaaag
    10981 aagcgcgccg caaacgcgtt caaaatcaag aagaataacc atgccgtctg aagccttcag
    11041 acagcattcg acagaaacac ggaaaatatc atgtcaaaca aaatcaaaat gcttgtcgga
    11101 ttgggcaacc ccggcaaaga atacgaacaa acccgacaca atgcgggttt ttggttcctc
    11161 gacgaactgg cgtggaaatg gaaggcttca tttaaagaag aaaaaaaatt cttcggcgaa
    11221 gtcgcccgtg ccgccctgcc cgacggcgac gtttggctgc tcaaacctgc cacgttcatg
    11281 aaccgttccg gacaggcagt tgccgcgctt gcacagttct acaaaatcaa acccgaagaa
    11341 atcctcgtcg tccacgacga actcgacatt ccctgcggac ggattaaatt caaactcggc
    11401 ggcggcaacg gcggacacaa cggcttgaaa gacattcagg caaaactcgg cacggcagac
    11461 tattaccgcc tgcgcctcgg tatcgaccac ccgggcgacc gcaacctcgt cgtcggctat
    11521 gtcctgaaca aacccagtac ggaacaccgc cgacagattg acgatgccgt cgccaaatcc
    11581 ctgcaagcca tacccgacat ccttgccggc aaatgggaag aagcaacccg cttcctgcac
    11641 agcaaatgac ccgatgccgt ctgaagccct ttcagacggc atgttcccga tttccatatc
    11701 cgaacagtca tgaccgaact caagcagctt atccaaaccg aatccatccc cgtcatcgaa
    11761 gaaaccctcg atttcctgct gtacgaatgc agcatcgacg acgcaccatc cgccgaagaa
    11821 gtggcacaat ggcgcgacat acttgccgca cgcggcggca aattcctgcg cctgtccaaa
    11881 atctgccaaa cgtggctgga cgaggaggcg gcatgaagct gccgcgcaac cgcttcagcc
    11941 tgctttccgc attgtggttt gccggcggca tctattcgct gctcttcaaa gctgccgaca
    12001 ccgcgccgcc gccgtttccg catttcgaca aagcagcaca ccttgccctg tttttcgcac
    12061 aaatctggct tttgaccaaa gcattcaaaa ccggaaaact tcccatcccc taccgcagcc
    12121 tgatggtctt tgccctctgt ttcgccctct tcagcgaatg cgcgcaggca tggtttaccg
    12181 caacgagaac cggcagtttg ggcgatgttc ttgccgatat ggcaggtacg gttctcgcac
    12241 tctttgccgc ccgcgccgcc gaccgcccgg actgaatcgg tttatttccc ccaaacagga
    12301 tgcacctctt cccgatacat cctatttttc cccttacata tcaatcccat ctcataaaca
    12361 aaaccgacaa atcgtttaca atatatttac actacatcag attacaaata tactcgaacc
    12421 agttgtagaa tcggcggttc cacaaaccgt ttcggattgc acgacacaaa agaaaaaccg
    12481 attttattgc cttaaaggag tattcatgaa cctgcatgca aaggacaaaa cccagcatcc
    12541 cgaaaacgtc gagctgctca gtgcgcagaa gccgattacc gactttaagg gcctgctgac
    12601 caccattatt tccgccgtcg tctgtttcgg catttaccac atcctgcctt acagccccga
    12661 tgccaataaa ggtatcgcgc tgctgatttt cgttgccgca ctttggttta ccgaagccgt
    12721 ccacattacc gtaaccgcac tgatggtgcc gattctcgcc gtcgtactcg gtttccccga
    12781 catggacatc aaaaaggcga tggctgattt ttccaacccg attatctaca tttttttcgg
    12841 aggcttcgcg cttgccaccg ccctgcatat gcagcggctg gaccgtaaaa tcgccgtcag
    12901 cctgttgcgc ctgtcgcgcg gcaatatgaa agtggcggtt ttgatgttgt tcctcgttac
    12961 cgcctttctg tccatgtgga tcagcaacac cgccaccgcc gcgatgatgc tgcctctagc
    13021 aatgggtatg ctgagccacc tcgaccagga aaaagaacac aaaacctacg tcttcctcct
    13081 gctcggcatc gcctattgcg ccagcatcgg cggcttgggc acgctcgtcg gctcgccgcc
    13141 caacctgatt gccgccaaag ccctaaatct ggacttcgtc ggctggatga agctcggcct
    13201 gccgatgatg ctgttgattc tgcccttgat gctgctctcc ctgtacgtca tcctcaaacc
    13261 taatttgaac gaacgcgtgg aaatcaaagc cgaatccatc ccttggacgc tgcaccgcgt
    13321 gatcgcgctg ttgattttcc ttgccacagc cgccgcgtgg atattcagct ccaaaatcaa
    13381 aaccgccttc ggcatttcca atcccgacac cgttatcgcc ctgagtgccg ccgtcgccgt
    13441 cgtcgtcttc ggcgtggcgc aatggaagga agtcgcccgc aataccgact ggggcgtgtt
    13501 gatgctcttc ggcggcggca tcagcctgag cacgctgttg aaaacatccg gcgcgtccga
    13561 agccttggga cagcaggttg ccgccacctt ttccggcgcg cccgcatttt tggtgatact
    13621 catcgtcgcc gccttcatta tttttctgac cgagttcacc agcaacaccg cctccgccgc
    13681 attgcttgta ccgattttct ccggcatcgc tatgcagatg gggctgcccg aacaagtctt
    13741 ggtattcgtc atcggcatcg gcgcatcttg tgccttcatg ctgccggttg ccacaccgcc
    13801 taacgcgatt gtgttcggca cgggcttaat caagcaacgc gaaatgatga atgtcggcat
    13861 actgctgaac atcctctgcg tagtattggt tgctctgtgg gcttatgctg tactgatgta
    13921 aaccatcgac ctaaacaaca agaccgtctg aaagaatatt tttcagacga ccttgaagtt
    13981 tgtcgttcag acacaatttg tcgaatcatt caaaaccgga ttctaacgaa aggaaaccca
    14041 tgattatcct gcacaccaac aaaggcgaca tcaaaatcga actcgatttc gacaaagccc
    14101 ctgttaccgc caaaaacttc gagcaatacg tcaaagacgg cttctacgac ggcgtaatct
    14161 tccaccgcgt catcaaaggc ttcatgattc aaggcggcgg catggatgaa aacatgaacg
    14221 aaaaagaaac ccgcgatccg attcaaaacg aagcgtccaa cggcctgccc aacgataaat
    14281 acaccatcgc catggcacgc acttccgacc cccattccgc cagcgcgcaa ttcttcatca
    14341 acactgccga caacgctttc ctgaacttcc gttctaaaga gctgtacggc aaaaccgtcg
    14401 tccaagactg gggctatgcc gtattcggta aagtcgttga cggttttgat gttgtcgatg
    14461 ccatcgaagg cgtatccacc aaacgccacg gctaccatga cgacgtaccg agcgaacctg
    14521 tcgtcatcat taaagccgaa gcggtataaa ccggcaatcc gaaagcagcc tgcacaaagg
    14581 ctgctttttt tgtttcaggc ggcataaaat ccaagcacat atgccgtctg aacacctccc
    14641 gcaagccttc ggacaatgtt gcaacgtgtt aaaatagcct cctttcctga aaccacaaca
    14701 aggaaaccat catggcaaat attgcccgcg acatcttcaa agcctacgac atccgaggca
    14761 ttgtcggcaa aaccctgacc gacgatgccg cctatctcat cggcagggcc atcgccgcca
    14821 aagccgccga aaaaggcatg acccgcatcg cgctcggacg cgacggacgc ttgagcggtc
    14881 ccgaactgat ggagcacatc caacgcggct ttaccgacag cggcatcgac gtcctcaacg
    14941 tcggtatggt cgccacgccc atgctctatt ttgccgccat caacgaatgc ggcggcagcg
    15001 gcgtgatgat taccggcagc cacaatccgc ccgattacaa tggtttcaaa atgatgctcg
    15061 gcggcgacac gctcgcaggc gaagccattc aagaactttt agccattatt gaaaaagacg
    15121 gttttgttgc cgccgacaaa caaggcaacg taaccgaaaa agacatctcc ggcgaatacc
    15181 acaaccacat cgtcggccac atcaaactca aacgcccgat gaacatcgct atcgacgcgg
    15241 gcaacggcgt gggcggcgcg tttgccggca aactctataa aggcttgggc aacgaagtaa
    15301 ccgagctttt ctgcgacgtg gacggcaatt tccccaacca ccatcccgac ccatccaaac
    15361 ccaaaaacct gcaagatttg attgccgcgc tgaaaaacgg cgatgccgaa atcggcttgg
    15421 cgtttgacgg cgatgccgac cgcttgggcg tagttaccaa agacggcaac atcatctatc
    15481 cagaccgcca actgatgctt ttcgcgcaag acgtgttgag ccgcaatcct aaagccaaag
    15541 taattttcga cgtcaaatcc acccgtctgc ttgcgccttg gattaaagaa cacggcggcg
    15601 aacctgtgat ggaaaaaacc ggccacagct ttatcaaatc ctccatgaaa aaaaccggcg
    15661 cgccggttgc cggcgaaatg agcggacaca tcttcttcaa agaacgctgg ttcggcttcg
    15721 acgacggtct gtacgccggc gcacgcctct tggaaatcct gtctgcctcc gataatccgt
    15781 ccgaagtgtt aaacaacctg ccgcaaagca tttccacgcc cgaactcaac atcgccctgc
    15841 ccgaaggcag caacggccat caggttatcg acgaactcgc cgccaaagcc gaatttgaag
    15901 gcgcaaccga aatcatcacc atcgacggtc tgcgcgttga atttcccgac ggcttcggtc
    15961 tgatgcgtgc ttccaatacc acgccgattc tggtgttgcg ttttgaggcg gatacgcaag
    16021 aagccatcga gcggattcaa aaccaattca aagccgtcat cgaaagcaat ccgaatctaa
    16081 tctggcctct gtaaacacag gaaaaatgct taatcaaatc ttggtagatt ggatttggct
    16141 taaaaatctc cgcgtccgtt cgtgtttggg gtggtcgaac aactctttcg ggctgccctg
    16201 ctctacgata acgccgccgt ccatcacgac aacggtcgtg gcaacttcca gcgcgaactt
    16261 gatttcgtgg gtaacgacga ccatcgtcca accttcccgc gccaattcct tcatggcgtt
    16321 caacacgtct tgcaccaatt cagggtcgag tgcggaagtg ggttcgtcaa acaacatcag
    16381 ctcgggctga atcgccaatg cgcgggcaat gccgacgcgc tgctgctgac cgccggaaag
    16441 ctggtaggga tacaaatcca ctttgtcgcc caagccgact ttttccagca gtttcagagc
    16501 ctcttcgcgc gcttgggcgg caggcttgcc ctgtacggca accggtcctt ccattacgtt
    16561 ttccaaggcg gttttgtgcg gaaagaggtt gtattgttga aacaccatgc ctgatttgcg
    16621 gcgcagtgcc aaaatatcgt gtttgcttgg ttttttagaa aaatcgattt tcagcggtcg
    16681 ctcgttgtcg aactcgattt gtccgtcttc gggcatttcc aacgcgttta ggcatcgcag
    16741 aaacgtcgtt ttgcctgagc cggaaggccc gaggataacg accacctgcc ctttgcccac
    16801 atccaaatcg atgccgcgca aaatagtgtt ttcgccgaag gtcttatgga tattgcggat
    16861 tttaatcata acaactcctt atttggcgac ataacggtcg aaacgttttt ccaaacgtgc
    16921 ctgaatcagg aacagaacct tgcagaaaca ccagtaaacc aatgcggctt cgatatagac
    16981 gggcaaaaag tcgtaagtgc ggtttgccgt ttcctgcgcg acgcggaaca attcagttac
    17041 cgttaccacc gctgcgagcg aggtgttttt gaacaagccg ataaactcgt tgctcaaagg
    17101 cggcacggca acgcggaatg cctgcggcgc gacgatgcgg cggaacgtct gcatataggt
    17161 cataccgatg gagaaacctg cttcccattg gcctttaggt acggacaaaa ttgccgcgcg
    17221 tatggtttcg gaagcgtatg caccgacatt gagcgagaag ccgataatgg cggcgggaat
    17281 cggatcgata tagataccga cggacggcag cccgtaaaac acaatcacaa gctgcaccaa
    17341 cagcggcgta ccgcgaatga cggaaatata aaattccacc aatttcagca ggattttccg
    17401 cacgatgccg ccggcgggca taatccgcac caaagccacg gctaccgcaa tcatcatacc
    17461 gataacgaaa gaagctgccg ccaaaggcag agagaccgcg aagccggctt tgaccatagg
    17521 caaaaacgcg ctgacaatca tatcggcgcg tgtttccgtc ataaacggca gcgaagcaag
    17581 gaaattattg aacactgatg tcttttccga agaattgttc gcccagtttt ttcagcgtac
    17641 cgtcggcttt cagctcgttg attgccgtac tgaatttcgc cacggcttcg tcattgccct
    17701 tgttgacaat caggccggaa ccgacttttt catcggcagg tgcggaccaa acgattttca
    17761 cgcccgcatt cgggtttttc ttcagatagt ccaaaaccgc caattcgtcg ttcagggttg
    17821 catcggcacg tttttgttca atcagggtca gcgattgcgc caaaccgtca acagccacca
    17881 aatctgcgcc tgcagctttg gctttttcgc cgtagttgct ggtcagggat tgtgcggttt
    17941 tcacgccttt gatgtcgtcg atagatttga tgttgctgtc gttacgggca accaatacgg
    18001 caccgctcca gctgtaagga tcggatttgt cgaatgtcgc ttggcgttcg gggctggtca
    18061 gaccgacttg gtttgccacc acgtcgaaac gccccgcctt caaacccg
//
LOCUS       DAWHSK010000014        16973 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.014, whole genome shotgun sequence.
ACCESSION   DAWHSK010000014 DAWHSK010000000
VERSION     DAWHSK010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16973
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.014"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..1719)
                     /gene="dxs"
                     /locus_tag="WIC03_000365"
     CDS             complement(<1..1719)
                     /gene="dxs"
                     /locus_tag="WIC03_000365"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEZ3882921.1"
                     /translation="MNPSPLLDLIDSPQDLRRLDKKQLPRLAGELRTFLLESVGQTGG
                     HFASNLGAVELTVALHYVYNTPEDKLVWDVGHQSYPHKILTGRKNQMHTMRQYGGLAG
                     FPKRCESEYDAFGVGHSSTSIGAALGMAAADKQLGSDRRSVAIIGDGAMTAGQAFEAL
                     NCAGDMDVDLLVVLNDNEMSISPNVGALPKYLASNVVRDMHGLLSTVKAQTGKVLDKI
                     PGAMEFAQKVEHKIKTLAEEAEHAKQSLSLFENFGFRYTGPVDGHNVENLVDVLKDLR
                     SRKGPQLLHVITKKGNGYKLAENDPVKYHAVANLPKESAAQMPSENKPAAKPTYTQVF
                     GKWLCDRAAADSRLVAITPAMREGSGLVEFEQRFPDRYFDVGIAEQHAVTFAGGLACE
                     GMKPVVAIYSTFLQRAYDQLVHDIALQNLPVLFAVDRAGIVGADGPTHAGLYDLSFLR
                     CVPNMIVAAPSDENECRLLLSTCYQADAPAAVRYPRGTGTGVPVSDGMETVEIGKGII
                     RREGEKTAFIAFGSMVAPALAVAEKLNATVADMRFVKPIDEELIVRLARSHDRIVTLE
                     ENAEQGG"
     gene            complement(1804..2721)
                     /gene="xerC"
                     /locus_tag="WIC03_000366"
     CDS             complement(1804..2721)
                     /gene="xerC"
                     /locus_tag="WIC03_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEZ3882922.1"
                     /translation="MVLDGFAAHFDAYLENIVREGKSEHTVAAYRRDLEELFALLAQM
                     PSEDAGGVPQDLSRRDFTAALRRLSQRGLNARTLARKLSSWRQYCVWLVERGLLHTDP
                     TADIKPPKQPERVPKALPQEWLNRMLDLPVDGGDPLAVRDHALFELMYGSGLRVSEIH
                     GLNADDVYLDEAWVHVTGKGRKQRQVPLTGKSVEALKNYLPLRQTASDGKALFTGRNG
                     TRLSQRQIQKRLAQWAAQNGDGRHVSPHMMRHSYAGHLLQASRDIRAVQELLGHSSLS
                     TTQIYTKLDFDHIARLYDEAHPRAKRQDE"
     gene            2915..3979
                     /locus_tag="WIC03_000367"
     CDS             2915..3979
                     /locus_tag="WIC03_000367"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase class II"
                     /protein_id="HEZ3882923.1"
                     /translation="MALVSMRQLLDHAAENSYGLPAFNVNNLEQMRAIMEAADQVNAP
                     VIVQASAGARKYAGAPFLRHLILAAVEEFPHIPVVMHQDHGASPDVCQRSIQLGFSSV
                     MMDGSLMEDGKTPSSYEYNVNATRTVVNFSHACGVSVEGEIGVLGNLETGEAGEEDGV
                     GAVGKLSHDQMLTSVEDAVRFVKDTGVDALAIAVGTSHGAYKFTRPPTGDVLRIDRIK
                     EIHQALPNTHIVMHGSSSVPQEWLKVINEYGGNIGETYGVPVEEIVEGIKHGVRKVNI
                     DTDLRLASTGAVRRYLAENPSDFDPRKYLSKTIEAMKQICLDRYLAFGCEGQAGKIKP
                     VSLEKMANRYAKGELNQIVK"
     gene            3999..4958
                     /locus_tag="WIC03_000368"
     CDS             3999..4958
                     /locus_tag="WIC03_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="factor H binding family protein"
                     /protein_id="HEZ3882924.1"
                     /translation="MPSEPLFGRHLIFASLTCLIDAVCKKRYHNQNVYILSILRMTRS
                     KPVNRTAFCCFSLTAALILTACSSGGGGVAADIGAGLADALTAPLDHKDKSLQSLTLD
                     QSVRKNEKLKLAAQGAEKTYGNGDSLNTGKLKNDKVSRFDFIRQIEVDGQLITLESGE
                     FQIYKQDHSAVVALQIEKINNPDKIDSLINQRSFLVSGLGGEHTAFNQLPSGKAEYHG
                     KAFSSDDAGGKLTYTIDFAAKQGHGKIEHLKTPEQNVELASAELKADEKSHAVILGDT
                     RYGGEEKGTYHLALFGDRAQEIAGSATVKIREKVHEIGIAGKQ"
     gene            5097..5774
                     /gene="tsaB"
                     /locus_tag="WIC03_000369"
     CDS             5097..5774
                     /gene="tsaB"
                     /locus_tag="WIC03_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEZ3882925.1"
                     /translation="MQADFNRPVLAIDTGTSYLSLALRADGETHLFHQEAGSRQSELI
                     LPEIRTLFHDAGITAADLGAIVYAKGPGAFTGLRIGIGVAQGLATPFDTPLIGIPTLD
                     AAASLPPPQSCILAAADARMGEVFYAWFDTLNRRRLSGYQVGRAADIRLPEGCAFSDG
                     IGNAFALENRPPFSGKPDMPTAADFLNLAEEGGFPAVAAAHAELLYVRNKIALTAKEQ
                     AERRALL"
     gene            5771..6211
                     /gene="rimI"
                     /locus_tag="WIC03_000370"
     CDS             5771..6211
                     /gene="rimI"
                     /locus_tag="WIC03_000370"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEZ3882926.1"
                     /translation="MNIRRAVYADCAALAALDAVCNPSAWTQHQFESALVSPSEQVFL
                     AEKDGRLAAFIVWQNLPDESELHLIATAPECRRRGVASALLEYWYAHLPEGTQRLLLE
                     VRAGNTAAQALYAAHGFSIAGRRKNYYRTADGKTEDAVLMEKIC"
     gene            6205..6942
                     /locus_tag="WIC03_000371"
     CDS             6205..6942
                     /locus_tag="WIC03_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEZ3882927.1"
                     /translation="MLSARYLHLHEALGLGPMWLKQAAAVLPPKNTPATPAQARPQTV
                     RAALIRPSQPHNGQARLETMKALETAAVPTRKPAPETETPLPGLSDGIAPVPAASGIT
                     KLAVVSLCPPIEDAIYGQLFHGKAGVLLDNILKAVGLDAAYVHKTCWVKTAAVGNPMP
                     SEAAIETATVSVMQELDGCRAPAVLFLGQAFVNPERQAMIETLCGSRPFFIIDHPARL
                     LRQPELKARAWQVLKQLKRALRQGGGS"
     gene            7004..7645
                     /gene="pyrE"
                     /locus_tag="WIC03_000372"
     CDS             7004..7645
                     /gene="pyrE"
                     /locus_tag="WIC03_000372"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEZ3882928.1"
                     /translation="MTDFRQDFLKFSLAQNVLKFGEFTTKAGRRSPYFFNAGLFNDGL
                     STLQLAKFYAQSIIESGIRFDMLFGPAYKGIILAAATAMMLAEKGVNVPFAYNRKEAK
                     DHGEGGVLVGAPLKGRVLIIDDVISAGTSVRESIKLIEAEGATPAGVAIALDRMEKGT
                     GELSAVQEVEKQYGLPVAPIASLNDLFILLQNNPEFGQFLEPVRAYRRQYGVE"
     gene            7719..8153
                     /locus_tag="WIC03_000373"
     CDS             7719..8153
                     /locus_tag="WIC03_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3882929.1"
                     /translation="MPACFCPHCKTRLWVKETQLNVAQGFVVCQKCEGLFKAKDHLAS
                     TKEPIFNDLPEAVSDVKLVHRIGTRAIGKKQISRDEIAGILNGGTTQPDIPPATAATP
                     AAAPQVTVPPAAPARQDGFNWTIATLFALIVLIMQLSYLVIL"
     gene            8150..9460
                     /gene="argA"
                     /locus_tag="WIC03_000374"
     CDS             8150..9460
                     /gene="argA"
                     /locus_tag="WIC03_000374"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HEZ3882930.1"
                     /translation="MSAPDLFVAHFREAAPYIRQMRGKTLVAGIDDRLLEGDTLNKLA
                     ADIGLLSQLGIRLVLIHGARHFLDHHAAAQGRTPHYCRGLRVTDETSLEQAQQFAGTV
                     RSRFEAALCGSVSGFARAPSVPLVSGNFLTARPIGVIDGTDMEYAGVIRKTDTAALRF
                     QLDAGNIVWLPPLGHSYSGKTFYLDMLQTAASAAVSLQAEKLVYLTLSDGIARPDGTL
                     AETLSAQEAQSLAEHAGSETQRLISSAVAALEGGVHRVQILNGAADGSLLQELFTRNG
                     IGTSIAKEAFVSIRQAHSGDIPHIAALIRPLEEQGILLHRSREYLENHISEFSILEHD
                     GNLYGCAALKTFAEADCGEIACLAVSPQAQDGGYGERLLAHIIDKARGIGISRLFALS
                     TNTGEWFAERGFQTASEAELPEARRKAYRNNGRNSHILVRRLHR"
     gene            9556..11571
                     /locus_tag="WIC03_000375"
     CDS             9556..11571
                     /locus_tag="WIC03_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEZ3882931.1"
                     /translation="MKSYPDPYRHFEDLDSAETQNFAAEANAETRARFLNNDKARALS
                     DGILAQLQDTRQIPFCQEHRARMYHFHQDAEYPKGVYRVCTAATYRSGYPEWKILFSV
                     ADFDELLGDDVYLGGVSHLVEKPNRALLTLSKSGGDAAYTLEVDLEAGELVEGGFHFP
                     AGKNHVSWRDENSVWVCPAWDERQLTESGYPREVWLVERGKSFEESLPVYQIDKDAMM
                     VNAWRYLDPQGSPIDLIEASDGFYTKTYLQVSSESEAKPLNLPNDCDVAGYLAGHLLL
                     TLRKDWNRANQSYPSGALVAVKLNRGELGAAQLLFAPDETQALESVETTKRFVVASLL
                     ENVQGRLKAWRFADGKWQEAELPRLPSGALEMTDQPWGGDVVYLAASDFTTPLTLFAL
                     DLNVMELTVMRRQPQQFDSDGINVQQFWTTSADGERIPYFHVGKNAAPDMPTLVYAYG
                     GFGIPELPHYLGSIGKYWLEEGNAFVLANIRGGGEFGPRWHQAAQGISKHKSVDDLLA
                     VVRDLSERGMSSPRHIGLQGGSNGGLITAAAFVCEPQSIGALVCEVPLTDMIRYPLLS
                     AGSSWTDEYGNPQKYEVCKRWLGELSPYHNLSDGIDYPPALITTSLSDDRVHPAHALK
                     FYAKLHETSAQSWLYSPDGGGHTGNGTQRESADELACVLLFLKEFLG"
     gene            complement(11685..12641)
                     /locus_tag="WIC03_000376"
     CDS             complement(11685..12641)
                     /locus_tag="WIC03_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ3882932.1"
                     /translation="MNTAAIYRQYQTYVRSDKSGWALDGCSDSALIAQAKQPGLHLEM
                     CINRFDSGITLSRMRGGGTGAFPTEIHNFSHNCALFVMVSGQNRLQMGGREYRPSAGE
                     IWLVRGELADVSETLLPDNSGMCALHLDFSLEKLRRWHDEGLLDERLFSPQTIGRFAL
                     QRLAQNAAALTAAACPLLQRPFESDGFGLLADEAAALELSARLLRFTFRRHDNGYRRR
                     RIDEAADILQHEFARPLTIAEIARRVGLNECYLKRYFKAQTGETVAGCLRRLRLEHAL
                     ALIESGSTVQAAMHFCGYRHAGRFNEAFRRHYGFLPSDVKKC"
     gene            12877..13842
                     /locus_tag="WIC03_000377"
     CDS             12877..13842
                     /locus_tag="WIC03_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ3882933.1"
                     /translation="MKPRFYWAACAVLLTACSPEPAAEKTVSAASASAATLTVPTARG
                     DAVVPKNPERVAVYDWAALDTLTELGVNVGATTAPVRVDYLQPAFDKAATVGTLFEPD
                     YEALHRYNPQLVITGGPGAEAYEQLAKNATTIDLTVDNGNIRTSGEKQMETLARIFGK
                     EARAAELKAQIDALFAQTREAAKGKGRGLVLSVTGNKVSAFGTQSRLASWIHGDIGLP
                     PVDESLRNEGHGQPVSFEYIKEKNPDWIFIIDRTAAIGQEGPAAVEVLDNALVRGTNA
                     WKRKQIIVMPAANYIVAGGSRQLIQAAEQLKAAFEKAEPVAAGKE"
     gene            complement(14149..14301)
                     /locus_tag="WIC03_000378"
     CDS             complement(14149..14301)
                     /locus_tag="WIC03_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3882934.1"
                     /translation="MRFVVLASFSGRSCNESVIDFDVSLRLTALSLTLSHRERGRVAV
                     ASLFAA"
     gene            14332..14655
                     /locus_tag="WIC03_000379"
     CDS             14332..14655
                     /locus_tag="WIC03_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003775941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEZ3882935.1"
                     /translation="MQNETINLKQHLAAIKEYWQPEIINRHGFQFRLVKLLGDYGWHT
                     HGYSDKVLFAVEGDMAVDFADGGSMTIREGEMAVVPKSVSHRPRSENGCSVVLIELSD
                     PSEAV"
     gene            14769..16946
                     /locus_tag="WIC03_000380"
     CDS             14769..16946
                     /locus_tag="WIC03_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEZ3882936.1"
                     /translation="MTRFKYSLLFAALLPVYAQADVSVSDDPKPQESTELPTITVTAD
                     RTASSNDGYTVSGTHTPLGLPMTLREIPQSVSVITSQQMRDQNIKALDRALLQATGTS
                     RQIYGSDRAGYNYLFARGSRIANYQINGIPVADALADTGNANTAAYERVEVVRGVAGL
                     LDGTGEPSATVNLVRKRPTRKPLFEVRAEAGNRKHFGLGADVSGSLNAEGTLRGRLVS
                     TFGRGDSWRQRERSRDAELYGILEYDIAPQTRVHAGMDYQQAKETADAPLSYAVYDSQ
                     GYATAFGPKDNPATNWANSRHRALNLFAGIEHRFNQDWKLKAEYDYTRSRFRQPYGVA
                     GVLSIDHNTAATDLIPGYWHADPRTHSASVSLIGKYRLFGREHDLIAGINGYKYASNK
                     YGERSIIPNAIPNAYEFSRTGAYPQPSSFAQTIPQYGTRRQIGGYLATRFRAADNLSL
                     ILGGRYSRYRTGSYDSRTQGMTYVSANRFTPYTGIVFDLTGNLSLYGSYSSLFVPQSQ
                     KDEHGSYLKPVTGNNLEAGIKGEWLEGRLNASAAVYRARKNNLATAAGRDPSGNTYYR
                     AANQAKTHGWEIEVGGRITPEWQIQAGYSQSKTRDQDGSRLNPDSVPERSFKLFTAYH
                     FAPEAPSGWTIGAGVRWQSETHTDPATLRIPNPAAKARAADNSRQKAYAVADIMARYR
                     FNPRAELSLNVDNLFNKHYRTQPDRHSYGALRTVNAAFTYRFK"
BASE COUNT         3764 a         5254 c         4682 g         3273 t
ORIGIN      
        1 gccgccctgt tcggcgtttt cttcaagggt aacgatgcgg tcgtggcttc gggcaaggcg
       61 gacaatcaac tcttcgtcta tcggtttgac gaagcgcata tcggcgacgg tggcgttcag
      121 tttttcggca accgccaatg cgggggcgac catactgccg aaggcaatga atgcggtttt
      181 ctcaccttcg cggcggataa tgcccttgcc gatttccacg gtttccatgc cgtctgaaac
      241 cggcacgccc gtacccgtgc cgcgcggata gcggacggcg gcgggcgcgt ctgcctgata
      301 gcaggtcgaa agcagcaggc ggcattcgtt ttcatcgctc ggcgcggcga caatcatgtt
      361 cggcacgcag cgcaaaaagc tcaaatcgta cagaccggcg tgggtcgggc cgtccgcgcc
      421 gacgatgccc gcacggtcga cggcaaacaa aacgggcagg ttttgcaggg cgatgtcgtg
      481 caccagttgg tcgtaggcgc gttgtaaaaa ggtggaataa atcgccacga cgggcttcat
      541 gccttcgcac gccaaaccgc cggcaaaggt aacggcgtgc tgctcggcaa taccgacatc
      601 gaaatagcgg tcggggaatc gttgttcaaa ctccaccagt ccgctgccct cgcgcatggc
      661 gggggtaatc gcaaccagtc gggaatctgc cgccgcccgg tcgcacagcc atttgccgaa
      721 tacttgggta taggtcggtt tggcggcagg tttgttttca gacggcattt gcgccgcgct
      781 ttctttgggc aggttggcga cggcgtggta tttgacggga tcgttttcgg cgagtttgta
      841 gccgttgcct tttttggtaa tgacgtgcag caactgaggg cctttgcggc tgcgcaagtc
      901 tttcaatacg tccaccagat tttcgacgtt gtgcccgtcc acgggaccgg tgtagcggaa
      961 gccgaagttt tcaaacagcg acagcgactg tttggcgtgt tcggcttctt cggcaagggt
     1021 tttgatttta tgttcgactt tttgggcaaa ctccatcgcg ccgggtattt tgtctaatac
     1081 cttgcccgtt tgcgctttga cggtactcaa cagtccgtgc atatcgcgca cgacgttgct
     1141 ggcaaggtat ttgggcaacg caccgacgtt gggggaaatc gacatttcgt tgtcgttgag
     1201 gacgaccagc aaatccacat ccatatcgcc cgcgcagttc aaggcttcaa acgcctgacc
     1261 cgccgtcatc gcgccgtcgc cgatgatggc gacgctgcgg cggtcgctgc ccaactgttt
     1321 gtccgccgcc gccatgccca acgccgcgcc gatggaggtg gaggaatgcc ccacgccgaa
     1381 cgcgtcgtac tcggactcgc aacgtttcgg aaaacccgcc aaaccgccat attggcgcat
     1441 ggtgtgcatc tggtttttac gtccggtaag aattttgtgc ggatagcttt ggtgtccgac
     1501 atcccacacc agcttgtctt cgggcgtgtt gtaaacgtag tgcagcgcaa ccgtcagctc
     1561 gaccgcgccc aaattgctgg cgaaatgccc gccggtctgc ccgacagatt ccagcagaaa
     1621 ggtgcgcaac tcgccggcaa ggcgcggcag ctgttttttg tccagacggc gcaaatcttg
     1681 cgggctgtca atcaggtcga gtagggggct tgggttcatg gtgtgtcttt tttatgtgtc
     1741 gtccgggtgc aacggccaat tatatatcaa gagcgtgcgg ctgacggctg attttgccgt
     1801 atgtcattcg tcctgccgct tggcgcgcgg gtgggcttcg tcatacaggc gggcgatgtg
     1861 gtcgaaatcg agcttggtat aaatctgcgt ggtcgaaagg ctgctgtgcc cgagcagctc
     1921 ctgcaccgcc ctgatgtcgc gcgaagcctg caataggtgt ccggcgtagc tgtggcgcat
     1981 catatgcggc gaaacgtgcc tgccgtcgcc gttttgcgcc gcccattgcg ccaaacgttt
     2041 ttggatttgg cgttggctca ggcgcgtgcc gttcctgccg gtaaacaggg ctttgccgtc
     2101 cgatgccgtc tgacgcagcg gcagatagtt tttcagggct tccacgcttt tgccggtcag
     2161 cggcacctgc cgctgcttgc gccctttgcc ggtaacgtgt acccacgcct cgtccaaata
     2221 gacatcatct gcattcaagc cgtgtatctc gctcacgcgc aaaccgctgc cgtacatcag
     2281 ttcgaacaga gcgtggtcgc gcaccgccag cgggtcgccg ccgtccacgg gcaaatccag
     2341 catccggttc agccattcct gcggcagggc tttgggtacg cgctcgggct gcttcggcgg
     2401 tttgatgtcg gcggtcgggt cggtatgcag cagaccgcgt tccaccagcc aaacacaata
     2461 ctgccgccaa gacgacagct tgcgcgccag cgtccgcgca ttcaaaccgc gctgcgacag
     2521 ccgccgcaac gccgccgtaa aatcgcgccg cgacaagtcc tgcggcacgc cgcctgcatc
     2581 ttcagacggc atttgtgcca acagcgcaaa cagttcttcc aaatcgcgcc ggtatgccgc
     2641 caccgtgtgc tccgacttgc cctcgcgcac gatgttttcc aaataagcgt caaaatgcgc
     2701 cgcaaacccg tccaaaacca taaccgcccc acacaaatat caaaaaaaca gtgaaaacat
     2761 tatcataaac gacacaccaa aaccccgaac cgcccaaaat ccgacccgtc cgcccgccgt
     2821 tatcgttaac ataaaatcaa tgagccgttt atggtttttt gccgcaaaaa acattataat
     2881 ccgccttatt tacctattgc ccaaggagac acaaatggca ctcgtatcca tgcgccaact
     2941 gcttgatcat gctgccgaaa acagctacgg cctgcccgcg ttcaacgtca acaacctcga
     3001 acaaatgcgc gccattatgg aagccgccga ccaagtcaac gcgcccgtca tcgtacaggc
     3061 gagtgcaggt gcgcgcaaat atgcgggcgc gccgtttttg cgccacctga ttctggcggc
     3121 agtcgaagaa tttccgcaca tccccgtcgt gatgcaccaa gaccacggcg catcgcccga
     3181 cgtgtgccaa cgctccatcc aactgggctt ttcctccgtg atgatggacg gctcgctgat
     3241 ggaagacggc aaaacccctt cttcttatga atacaacgtc aacgccaccc gtaccgtggt
     3301 taatttctcc cacgcttgcg gcgtatccgt cgaaggcgaa atcggcgtgt tgggcaacct
     3361 cgaaactggc gaagccggcg aagaagacgg tgtaggcgca gtgggcaaac tttcccacga
     3421 ccaaatgctc accagcgtcg aagatgccgt gcgtttcgtt aaagataccg gcgttgacgc
     3481 attggcgatt gccgtcggca ccagccacgg cgcgtacaaa ttcacccgtc cgcccacagg
     3541 cgacgtgttg cgtatcgacc gcatcaaaga aatccaccaa gccctgccca atacacacat
     3601 cgtgatgcac ggctccagct ccgttccgca agaatggctg aaagtcatca acgaatacgg
     3661 cggcaatatc ggcgaaacct acggcgtgcc ggttgaagaa atcgtcgaag gcatcaaaca
     3721 cggcgtgcgc aaagtcaaca tcgacaccga cttgcgcctt gcttccaccg gcgcggtacg
     3781 ccgctacctt gccgaaaacc cgtccgactt cgatccgcgc aaatatttga gcaaaaccat
     3841 tgaagcgatg aagcaaatct gcctcgaccg ttatcttgcg ttcggctgcg aaggtcaggc
     3901 aggcaaaatc aaaccggttt ccttggaaaa aatggcaaac cgttatgcca agggcgaatt
     3961 gaaccaaatc gtcaaataac aggttgcttg taaacaaaat gccgtctgaa ccgttgttcg
     4021 gacggcattt gatttttgct tctttgacct gcctcattga tgcagtatgc aaaaaaagat
     4081 accataacca aaatgtttat atattatcta ttctgcgtat gactaggagc aaacctgtga
     4141 accgaactgc cttctgctgc ttttctctga ccgccgccct gattctgacc gcctgcagca
     4201 gcggaggcgg cggtgtcgcc gccgacatcg gcgcggggct tgccgatgca ctaaccgcac
     4261 cgctcgacca taaagacaaa agtttgcagt ctttgacgct ggatcagtcc gtcaggaaaa
     4321 acgagaaact gaagctggcg gcacaaggtg cggaaaaaac ttatggaaac ggcgacagcc
     4381 ttaatacggg caaattgaag aacgacaagg tcagccgttt cgactttatc cgtcaaatcg
     4441 aagtggacgg gcagctcatt accttggaga gcggagagtt ccaaatatac aaacaggacc
     4501 actccgccgt cgttgcccta cagattgaaa aaatcaacaa ccccgacaaa atcgacagcc
     4561 tgataaacca acgctccttc cttgtcagcg gtttgggtgg agaacatacc gccttcaacc
     4621 aactgcccag cggcaaagcc gagtatcacg gcaaagcatt cagctccgac gatgctggcg
     4681 gaaaactgac ctataccata gatttcgccg ccaaacaggg acacggcaaa atcgaacact
     4741 tgaaaacacc cgagcaaaat gtcgagcttg cctccgccga actcaaagca gatgaaaaat
     4801 cacacgccgt cattttgggc gacacgcgct acggcggcga agaaaaaggc acttaccacc
     4861 tcgccctttt cggcgaccgc gcccaagaaa tcgccggctc ggcaaccgtg aagataaggg
     4921 aaaaggttca cgaaatcggc atcgccggca aacagtagca tttgaaaaat gccgtccgaa
     4981 cacgatagtt taccgttcgg acggcatttt tgcgccatac tgccctagga cacgacggca
     5041 cggcaggcaa gctgatttca ctatataatg ccgtctgaac caacgagaac cataccatgc
     5101 aagctgattt taaccgtccc gtcctggcca tcgataccgg tacttcctat ttgtcgctcg
     5161 cgctgcgcgc cgacggcgaa acccacctgt tccatcaaga ggccggcagc cgccaatccg
     5221 aactgatcct gccggaaatc cgcacgctgt ttcacgatgc gggcattacc gccgccgatt
     5281 tgggggcgat tgtttatgcg aaaggccccg gcgcgtttac cggactgcgt atcggtatcg
     5341 gcgtggcgca aggtttggca acgccgtttg atacgccttt aatcggcatc cccacgctcg
     5401 atgccgccgc ctcgctgccg ccgccgcaaa gctgcatact cgccgctgcg gacgcgcgta
     5461 tgggcgaagt gttttatgcg tggttcgaca cgctgaaccg ccgccgtttg agcggttatc
     5521 aagtcggtag ggcggcagac atccggctgc cggagggatg tgccttttca gacggcatcg
     5581 gcaatgcgtt cgcgctggaa aaccgtccgc ccttctcagg caaacccgat atgccgactg
     5641 ccgccgattt tctcaatctg gcggaagaag gcggttttcc cgccgtcgct gcggcacacg
     5701 ccgagctgct ctacgtccgc aacaaaatcg ccttgactgc caaagaacag gcggaacgga
     5761 gggcgctcct gtgaacatcc gccgcgccgt ttatgccgat tgcgcggcac tcgccgcact
     5821 cgatgccgtc tgcaacccgt ccgcgtggac gcaacaccaa tttgagtccg cattggtttc
     5881 gccgtccgaa caggttttcc ttgcggaaaa agacggcagg ttggccgcct ttatcgtttg
     5941 gcagaacctg cccgacgaat ccgaactgca cctgattgcc accgcgcccg aatgccgccg
     6001 ccggggtgtt gcgtccgccc tgctcgaata ttggtacgca cacctgcccg aaggcacgca
     6061 acgcctgctg ctcgaagtcc gcgcaggcaa caccgccgca caggcactgt acgcggcgca
     6121 cggcttcagc attgcgggca ggcggaaaaa ctattaccgt acagccgacg gtaaaaccga
     6181 agatgccgtc ttgatggaga aaatatgtta agcgcgcgct acctccacct gcacgaagct
     6241 ttgggtttag gcccgatgtg gctgaaacag gccgccgccg tcctgccgcc caaaaacaca
     6301 cccgcaaccc cggcacaggc acgtccccaa accgtccgcg ccgccctgat ccgcccttcc
     6361 caaccccata acggtcaggc gcggctcgaa acgatgaaag cgttggaaac cgccgccgta
     6421 cctacgcgca aacccgcgcc tgaaaccgaa acgcctctgc ccggcctttc agacggcatc
     6481 gcccccgttc ccgccgcttc gggcatcacc aagcttgccg tcgtcagcct ttgcccaccg
     6541 atcgaggatg cgatttacgg gcaactgttc cacggcaaag caggtgttct gcttgacaac
     6601 atactcaaag ccgtaggact ggatgccgct tatgtccaca aaacctgttg ggtgaaaacc
     6661 gccgctgtcg gcaacccgat gccgtctgaa gcggcaatcg aaaccgcgac ggtttccgtc
     6721 atgcaggaac tcgacggctg ccgcgccccc gccgtcctat tcctcggaca ggcgtttgtc
     6781 aacccggaac ggcaggcgat gattgaaact ttgtgcggca gccgtccctt cttcatcatc
     6841 gaccatcccg cccggctttt acgccaaccc gaactcaaag cccgcgcctg gcaggtgttg
     6901 aaacagttga aacgcgcctt gcggcaaggc ggcggcagtt gaagcgcacc gcacggggcg
     6961 gtagaatcgc aactgcgtcc caatatctga cagaaagcac aaaatgaccg atttccgcca
     7021 agatttcctt aaattctccc tcgcccaaaa tgttttgaaa ttcggcgaat tcaccaccaa
     7081 agccggacgg cggtcgccct atttcttcaa tgccggtctc tttaacgacg gcttgtccac
     7141 gctgcaactg gcaaaatttt acgcacaatc catcattgaa agcggcatcc gattcgatat
     7201 gctgttcggt cccgcctaca aaggcattat tttggcggcg gcaaccgcga tgatgctggc
     7261 ggaaaaaggc gtgaacgtcc cgtttgccta caaccgcaaa gaagccaaag accacggcga
     7321 aggcggcgtg ttggtcggtg cgccgcttaa agggcgcgtg ctgattatcg acgacgtgat
     7381 ttccgccggc acatccgtac gcgaatcgat caaattgatt gaagcggagg gtgcaacccc
     7441 cgccggtgtc gccatcgcgc tcgaccgcat ggaaaaaggc acgggtgaat tgagcgcggt
     7501 tcaggaagtg gaaaaacaat acggcctgcc cgtcgccccc atcgccagcc tgaacgattt
     7561 gtttatcctg ttgcaaaaca atcccgaatt cggacagttc ctcgaacccg tccgagccta
     7621 ccgtcggcag tacggcgtag aataaaaaca aagcatatgc cgtctgaacc gccttacgcc
     7681 tcagacggca tcaaacctga cacacacgag gaaataccat gcccgcctgt ttctgccccc
     7741 actgcaaaac ccgtctctgg gtcaaagaaa cccaactcaa tgtcgcccaa ggcttcgtcg
     7801 tctgccaaaa atgcgaagga ctgtttaaag ccaaagacca tctggcaagc acgaaagaac
     7861 ccatattcaa cgatttgccc gaggctgttt cggatgtcaa actcgttcac cgtatcggca
     7921 cgcgcgccat cggcaagaaa cagatttccc gtgacgaaat cgccggcatc ctcaacggcg
     7981 gcacaaccca gcccgatatt ccgcccgcaa ccgccgccac ccctgctgcc gcaccgcagg
     8041 ttaccgtacc gcccgccgcg cccgcccgtc aggatgggtt caactggacg attgcaaccc
     8101 tgtttgccct tatcgtcctc attatgcagc tttcctacct cgtcatccta tgagcgcgcc
     8161 cgacctcttt gtcgcccact tccgcgaagc cgccccctac atccgccaaa tgcgcggcaa
     8221 aacgctggtc gccggcatag acgaccgcct gctcgaaggt gataccttaa acaagctcgc
     8281 cgccgacatc gggcttttgt cgcaactggg catcaggctc gtcctcatcc acggcgcgcg
     8341 ccacttcctc gaccaccacg ccgccgcgca aggccgcacg ccgcattatt gcaggggctt
     8401 gcgcgttacc gacgaaacct cgctcgaaca ggcgcagcag tttgccggca ccgtccgcag
     8461 ccgttttgaa gccgcattgt gcggcagcgt gtccgggttc gcgcgcgcgc cttccgtccc
     8521 gctcgtatcg ggcaacttcc tgaccgcccg tccgataggt gtgattgacg gaaccgatat
     8581 ggaatacgcg ggcgttatcc gcaaaaccga caccgccgcc ctccgtttcc aactcgacgc
     8641 gggcaatatc gtctggctgc cgccgctcgg acattcctac agcggcaaga ccttctatct
     8701 cgatatgctt caaaccgccg cctccgccgc cgtctcgctt caggccgaaa aactcgttta
     8761 cctgaccctt tcagacggca ttgcccgccc cgacggcaca ctcgccgaaa ccctctcggc
     8821 gcaggaggcg caatcgctgg cggaacacgc cggcagcgaa acgcaacggc tgatttcgtc
     8881 cgccgttgcc gcgctcgaag gcggcgtgca tcgcgtccaa atcctcaacg gggccgccga
     8941 cggcagcctg ctgcaagaac tcttcacccg caacggcatc ggcacgtcca ttgccaaaga
     9001 agccttcgtc tccatccggc aggcgcacag cggcgacatc ccgcacattg ccgccctcat
     9061 ccgcccgctg gaagaacagg gcatcctgct gcaccgcagc cgcgaatacc tcgaaaacca
     9121 catttccgaa ttttccatcc tcgaacacga cggcaacctg tacggttgcg ccgccctgaa
     9181 aacctttgcc gaagccgatt gcggcgaaat cgcctgcctt gccgtctcgc cgcaggcaca
     9241 ggacggcggc tacggcgaac gcctgcttgc ccacattatc gataaggcgc gcggcatagg
     9301 cataagcagg ctgttcgccc tctccacaaa taccggcgaa tggtttgccg aacgcggctt
     9361 tcagacggca tcggaagccg agttgcccga agcgcggcgc aaagcctacc gcaacaacgg
     9421 acggaactcg catattctgg tgcgtcgcct gcaccgctga ccgcaacgga aagccgccgc
     9481 agaaatgccg tctgaacccc gtttcagacg gcatttcccc gattataaag acctgcccaa
     9541 ccctcaagga ccccgatgaa atcctacccc gacccctacc gccattttga agacctcgat
     9601 tccgccgaaa cgcaaaactt cgctgctgaa gcgaatgccg aaacgcgcgc gcgtttttta
     9661 aacaacgaca aggcgcgcgc attgtctgac ggtattttgg cgcagttgca ggacacgcgg
     9721 cagattccgt tttgtcagga acaccgtgcg cggatgtacc atttccatca ggacgcggaa
     9781 tatccgaagg gcgtgtaccg cgtgtgcacg gcggcgacgt atcgttccgg ctatcccgag
     9841 tggaaaatcc tgttttcagt ggcggatttc gacgagctgc tcggtgacga tgtgtatttg
     9901 ggcggcgtgt cgcacttggt ggaaaagccc aaccgcgcgt tgttaacact gagcaaatcg
     9961 ggcggcgatg cggcgtacac gctggaagtg gatttggaag caggggagtt ggtagaaggc
    10021 ggttttcact ttccggcagg taaaaaccat gtgtcgtggc gcgatgaaaa cagcgtatgg
    10081 gtgtgtccgg cttgggacga acgccagttg accgaatcgg gctatccgcg cgaggtgtgg
    10141 ctggtggaac gcggcaaaag tttcgaggaa agcctgcctg tgtatcaaat cgataaagat
    10201 gcaatgatgg tgaacgcgtg gcgttatctc gatccgcagg gttcgccgat tgatttgatt
    10261 gaagcgtcgg acggttttta caccaaaacc tatttgcagg tgtcgtcgga aagcgaggcg
    10321 aaaccgttaa acctgcccaa cgattgcgac gtggccggct atctggccgg acatcttttg
    10381 ctgacgttac gtaaagactg gaaccgcgcg aaccaaagct atccgagcgg cgcattggtg
    10441 gcggtgaagc tgaatcgggg cgaactcggg gcggcacagc ttttgtttgc gcccgatgaa
    10501 acgcaggcat tggaaagcgt ggaaacgacc aagcgttttg tggtggcgag cctgctggaa
    10561 aacgtacaag gccgtctgaa agcgtggcgg tttgccgatg gcaaatggca ggaagctgag
    10621 ttgccgcgcc tgccttcggg cgcgttggaa atgaccgatc agccttgggg cggcgacgtg
    10681 gtttaccttg ccgccagcga tttcaccacg ccgctgacgc tgtttgcgct ggatttgaac
    10741 gtgatggaac tgaccgtcat gcgccgccag ccgcagcagt ttgattcaga cggcattaac
    10801 gtgcagcagt tttggacgac ttcggctgac ggcgagcgca ttccttattt ccacgtcggc
    10861 aaaaacgccg cgcccgacat gccgacgctg gtctatgcct acggcggttt cggcattccc
    10921 gaattgccgc attatctggg cagcattggc aaatattggc tggaagaggg caatgccttt
    10981 gtattggcga acatccgcgg cggcggcgag ttcggcccgc gctggcatca ggcggcgcag
    11041 ggcatcagca aacataaaag cgttgatgat ttattggcag tcgtgcgcga tttgtccgaa
    11101 cgcggcatga gttcgcccag acacatcggt ttgcagggcg gcagcaacgg cggcctgatt
    11161 accgccgctg ccttcgtgtg cgaaccgcaa agcatcggcg cgctggtgtg cgaagtaccg
    11221 ctgaccgaca tgatccgtta tccgctattg tccgccggtt caagttggac ggacgaatac
    11281 ggcaatccgc aaaaatacga agtctgcaaa cgctggctgg gcgaattgtc gccgtatcac
    11341 aatctttcag acggcatcga ttatccgcct gcgctcatta ccaccagcct cagcgacgac
    11401 cgcgtccatc ctgcccacgc gctcaagttt tacgccaaac tgcatgaaac ctcggcgcaa
    11461 tcttggctct actcgcctga cggcggcggc cataccggca acggcaccca acgcgaatcc
    11521 gccgacgaac tcgcctgcgt cttgctgttt ttgaaagagt ttttgggcta agggcgggga
    11581 gcggcactgc cgccgcgaat gaaaaaaggt cgtctgaaac tgctttttca gacgaccttt
    11641 tttaatggtt gtttcaaatc aaaatatcta tgccgccggc cccatcagca cttcttcaca
    11701 tccgaaggca aaaatccgta atgccgtctg aacgcttcgt tgaaccgtcc cgcgtggcgg
    11761 tagccgcaaa agtgcatggc ggcttggacg gtgctgccgg attcgatgag ggcgagcgcg
    11821 tgttccagcc gcaggcggcg caggcatccg gcgacggttt caccggtttg cgctttgaaa
    11881 tagcgtttca ggtagcattc gttcagtccg acgcggcggg cgatttcggc gatggtcagc
    11941 ggacgggcga attcgtgttg caggatgtcg gcggcttcgt ctatgcgccg gcggcggtaa
    12001 ccgttgtcgt ggcggcggaa ggtgaagcgc aataatcggg cggagagttc cagcgcggcg
    12061 gcttcgtcgg caagcaggcc gaagccgtcc gattcgaacg ggcgttgcag cagtgggcag
    12121 gccgccgccg tcagtgccgc tgcgttttgc gccagccgtt gcagggcgaa tcggcctatt
    12181 gtttgcggcg aaaacaggcg ttcgtccagc aagccttcgt cgtgccagcg gcgcagtttt
    12241 tccagcgaaa aatccaaatg cagcgcgcac atgccgctgt tgtcgggcag cagggtttcg
    12301 gatacgtccg ccaactcgcc gcgtaccagc cagatttcgc cggcagacgg gcggtattcc
    12361 ctgccgccca tttgtaaccg gttctgcccc gacaccatga cgaacaaggc gcagttgtgg
    12421 ctgaaattgt ggatttcggt gggaaacgcg cccgttccgc cgccgcgcat ccgcgacaag
    12481 gtgatgcccg agtcgaagcg gttgatgcac atttccagat gcaaaccggg ctgttttgcc
    12541 tgcgcaatga gcgcgctgtc ggaacagccg tccaacgccc agccggattt atcggagcgg
    12601 acataggttt ggtactggcg gtagatggcg gcggtgttca tgattggata ggaacgagtt
    12661 gtctaacaaa tgaattaaat aggaattatt accaataatc aagcgcaggg attggttgaa
    12721 acggaaaagg tcgtctgaaa gggtgtttca gacgaccttt tccgtatcgg gaatttgttt
    12781 tgccgtatcg ggaattttgc gttttgcggc gtggtttctg caggttgttt gcttaataat
    12841 aaacattctt attcgtatgc aaaggaaccg cacaccgtga aaccgcgttt ttattgggca
    12901 gcctgcgccg tcctgctgac cgcctgttcg cccgaacctg ccgccgaaaa aactgtatcc
    12961 gccgcatccg catctgccgc cacactgacc gtgccgaccg cgcggggcga tgccgttgtg
    13021 ccgaagaatc ccgaacgcgt cgccgtgtac gactgggcgg cgttggatac gctgaccgaa
    13081 ttgggcgtga atgtgggcgc aaccaccgcg ccggtgcgcg tggattattt gcagcctgca
    13141 tttgacaagg cggcaacggt ggggacgctg ttcgagcccg attacgaagc cctgcaccgc
    13201 tacaatcctc agcttgtcat taccggcggg ccgggcgcgg aagcgtatga acagttggcg
    13261 aaaaacgcga ccaccataga tctgacggtg gacaacggca atatccgcac cagcggcgaa
    13321 aagcagatgg agaccttggc gcggattttc ggcaaggaag cgcgcgcggc ggaattgaag
    13381 gcgcagattg acgcgctgtt cgcccaaacg cgcgaagccg ccaaaggcaa aggacgcggg
    13441 ctggtgctgt cggttacggg caacaaggtg tccgccttcg gcacgcagtc gcggttggca
    13501 agttggatac acggcgacat cggcctaccg cctgtagacg aatctttacg caacgagggg
    13561 cacgggcagc ctgtttcctt cgaatacatc aaagagaaaa accccgattg gattttcatc
    13621 atcgaccgta ccgccgccat cgggcaggaa gggccggcgg ctgtcgaagt attggataac
    13681 gcgctggtac gcggcacgaa cgcttggaag cgcaagcaaa tcatcgtcat gcctgccgcg
    13741 aactacattg tcgcgggcgg ctcgcggcag ttgattcagg cggcggagca gttgaaggcg
    13801 gcgtttgaaa aggcagaacc cgttgcggcg gggaaagagt agggagtcgt ctgaaaacgg
    13861 agcttccgaa ggaagcgggg ggtttctgcg aagctaaagt gcggtttcaa cgaattgaaa
    13921 agcagcctgt attttgaaaa taccgctcaa gcaaacctac ggtttgccgc cctctcccta
    13981 gccctctccc acagggagag gggattgggt tgcaggctgc ctttaaggtt taggcaaatt
    14041 tttaacttcg ttgaagctcc gttttcagac gaccttttgg aacaccacag gcacacgcat
    14101 cgaacggctg aatcaaagat tcagaccgat ggcagtccgc acccgagttt atgcggcaaa
    14161 cagcgaggct acggcaaccc gccccctctc cctgtgggag agggttaggg agagggcggt
    14221 aagccgcagg cttacatcaa agtcgataac gctttcgtta caactccgcc cactgaacga
    14281 agccaaaacg acaaaccgca tcgtaaacca cccaacccat aggagaaccc catgcaaaac
    14341 gaaaccatca acctgaaaca gcaccttgcc gccatcaaag aatactggca gcccgaaatc
    14401 atcaaccgcc acgggttcca attccgtttg gtcaaacttt tgggcgatta cggctggcat
    14461 acgcacggat acagcgacaa agtgctgttt gccgtggagg gcgacatggc ggtggacttc
    14521 gccgacggcg gcagcatgac gatacgcgag ggcgagatgg cggtcgtgcc gaagtcggtg
    14581 tcgcaccgcc cgcgttcgga aaacggctgc tcggtggtgc tgattgagtt gtccgacccg
    14641 tccgaggccg tctgaaaacg aagtttccga aggaagctga gtttctgcga agctaaaagc
    14701 agcctgcacc ttcaatcaat atgccgaaaa tacaacccca ccgcacacca acacacaaag
    14761 gaaatcccat gacacgcttc aaatattccc tgctgtttgc cgccctgttg cccgtgtacg
    14821 cgcaggccga tgtttctgtt tcagacgacc ccaaaccgca ggaaagcact gaattgccga
    14881 ccatcaccgt taccgccgac cgcaccgcga gttccaacga cggctacact gtttccggca
    14941 cgcacacccc gctcgggctg cccatgaccc tgcgcgaaat cccgcagagc gtcagcgtca
    15001 tcacatcgca acaaatgcgc gaccaaaaca tcaaagcgct cgaccgcgcc ctgttgcagg
    15061 cgaccggcac cagccgccag atttacggct ccgaccgcgc gggctacaac tacctgttcg
    15121 cgcgcggcag ccgcatcgcc aactaccaaa tcaacggcat ccccgttgcc gacgcgctgg
    15181 ccgatacggg caatgccaac accgccgcct atgagcgcgt agaagtcgtg cgcggcgtgg
    15241 cggggctgct ggacggcacg ggcgagcctt ccgccaccgt caatctggtg cgcaaacgcc
    15301 cgacccgcaa gccattgttt gaagtccgcg ccgaagcggg caaccgcaaa catttcgggc
    15361 tgggcgcgga cgtatcgggc agcctgaatg ccgaaggcac gctgcgcggc cgcctggttt
    15421 ccaccttcgg acgcggcgac tcgtggcggc agcgcgaacg cagccgcgat gccgaactct
    15481 acggcatttt ggaatacgac atcgcaccgc aaacccgcgt ccacgcaggc atggactacc
    15541 agcaggcgaa agaaaccgcc gacgcgccgc tcagctacgc cgtgtacgac agccaaggtt
    15601 atgccaccgc cttcggcccg aaagacaacc ccgccacaaa ttgggcgaac agccgccacc
    15661 gtgcgctcaa cctgttcgcc ggcatcgaac accgcttcaa ccaagactgg aaactcaaag
    15721 ccgaatacga ctacacccgc agccgcttcc gccagcccta cggcgtagca ggcgtgcttt
    15781 ccatcgacca caacaccgcc gccaccgacc tgattcccgg ttattggcac gccgacccgc
    15841 gcacccacag cgccagcgtg tcattgatcg gcaaataccg cctgttcggc cgcgaacacg
    15901 atttaatcgc gggtatcaac ggttacaaat acgccagcaa caaatacggc gaacgcagca
    15961 tcatccccaa cgccattccc aacgcctacg aattttcccg cacgggcgcc tatccgcagc
    16021 catcatcgtt tgcccaaacc atcccgcaat acggcaccag gcggcaaatc ggcggctatc
    16081 tcgccacccg tttccgcgcc gccgacaacc tttcgttgat actcggcggc agatacagcc
    16141 gttaccgcac cggcagctac gacagccgca cacaaggcat gacctatgtg tccgccaacc
    16201 gtttcacccc ctacacaggc atcgtgttcg acctgaccgg caacctgtcg ctttacggct
    16261 cgtacagcag cctgttcgtc ccgcaatcgc aaaaagacga acacggcagc tacctgaaac
    16321 ccgtaaccgg caacaatctg gaagccggca tcaaaggcga atggcttgaa ggccgtctga
    16381 acgcatccgc cgccgtgtac cgcgcccgta aaaacaacct cgccaccgca gcaggacgcg
    16441 acccgagcgg caacacctac taccgcgccg ccaaccaagc caaaacccac ggctgggaaa
    16501 tcgaagtcgg cggccgcatc acgcccgaat ggcagataca ggcaggttac agccaaagca
    16561 aaacccgcga ccaagacggc agccgcctga accccgacag cgtacccgaa cgcagcttca
    16621 aactcttcac tgcctaccac tttgcccccg aagcccccag cggctggacc atcggcgcag
    16681 gcgtgcgctg gcagagcgaa acccacaccg accctgccac gctccgcatc cccaaccccg
    16741 ccgccaaagc ccgcgccgcc gacaacagcc gccaaaaagc ctacgccgtc gccgacatca
    16801 tggcgcgtta ccgcttcaat ccgcgcgccg aactgtcgct gaacgtggac aacctgttca
    16861 acaaacacta ccgcacccag cctgaccgcc acagttacgg cgcactgcgg acagtgaacg
    16921 cggcgtttac ctatcggttt aaataaggtc gtctgaaaac ggagtttctg cga
//
LOCUS       DAWHSK010000015        16725 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.015, whole genome shotgun sequence.
ACCESSION   DAWHSK010000015 DAWHSK010000000
VERSION     DAWHSK010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16725
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.015"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(51..1220)
                     /locus_tag="WIC03_000381"
     CDS             complement(51..1220)
                     /locus_tag="WIC03_000381"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEZ3882937.1"
                     /translation="MSEYLFTSESVSEGHPDKVADQVSDAILDAILAQDPKARVAAET
                     LVNTGLCVLAGEITTTAQVDYIKVARETIKRIGYNSSELGFDANGCAVGVYYDQQSPD
                     IAQGVNEGEGIDLNQGAGDQGLMFGYACDETPTLMPFAIYYSHRLMQRQSELRKDGRL
                     PWLRPDAKAQLTVVYDSETGKVKRIDTVVLSTQHDPSIAYEELKNAVIEQIIKPVLPS
                     ELLTDETKYLINPTGRFVIGGPQGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVD
                     RSAAYACRYVAKNIVAAGLATQCQIQVSYAIGVAEPTSISIDTFGTGKISEEKLITLV
                     REHFDLRPKGIVQMLDLLRPIYSKSAAYGHFGREEPEFTWERTDKAAALRAAAGL"
     regulatory      complement(1403..1513)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01725"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01725"
     gene            1566..2462
                     /locus_tag="WIC03_000382"
     CDS             1566..2462
                     /locus_tag="WIC03_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEZ3882938.1"
                     /translation="MFRLQFGLFPPLRTAMHILLTALLKCLSLLPLSCLHTLGNRLGH
                     LAFYLLKEDRARIVANMRQAGMNPDPKTVKAVFAETAKGGLELAPAFFRKPEDIETMF
                     KAVHGWEHVQQALDKHEGLLFITPHIGSYDLGGRYISQQLPFPLTAMYKPPKIKAIDK
                     IMQAGRVRGKGKTAPTSIQGVKQIIKALRSGEATIVLPDHVPSPQEGGEGVWVDFFGK
                     PAYTMTLAAKLAHVKGVKTLFFCCERLPGGQGFDLHIRPVQGELNGDKAHDAAVFNRN
                     AEYWIRRFPTQYLFMYNRYKMP"
     gene            complement(2506..3570)
                     /gene="tsaD"
                     /locus_tag="WIC03_000383"
     CDS             complement(2506..3570)
                     /gene="tsaD"
                     /locus_tag="WIC03_000383"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEZ3882939.1"
                     /translation="MLVLGIESSCDETGVALYDTERGLRAHCLHTQMAMHAEYGGVVP
                     ELASRDHIRRLVPLTEGCLAQAGASYGDIDAVAFTQGPGLGGALLAGSSYANALALAL
                     DKPVIPVHHLEGHLLSPLLAEEKPDFPFVALLVSGGHTQIMAVRGIGDYALLGESVDD
                     AAGEAFDKTAKLLGLPYPGGAKLSELAESGRPEAFVFPRPMIHSDDLQMSFSGLKTAV
                     LTAVEKVREANGSETIPEQTRNDICRAFQDAVVDVLAAKVKKALLQTGFRTVVVAGGV
                     GANRKLRETFGNMTVQIPTPKGKPKHPSEKVSVFFPPTAYCTDNGAMIAFAGAMHLGK
                     GREVGAFNVRPRWPLSEIVR"
     gene            complement(3687..4874)
                     /gene="ccsB"
                     /locus_tag="WIC03_000384"
     CDS             complement(3687..4874)
                     /gene="ccsB"
                     /locus_tag="WIC03_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HEZ3882940.1"
                     /translation="MTEHYKTLPEHELLIQKSLISNLNLWDWVFAVLVFAATVFVQTR
                     SGMHMDIYETVMLWASAGIAVFLGWFFKPMRWFVPLSVLLAYAAVGLYGGNIKSAEIF
                     LLRYFLSSQSAIMWQCAFVFFALFAYISGAVLASVKNVPTNTLLGMGTVFAWVSAVAG
                     FTGLLVRWHESYLLRPDAGHIPVSNLYEVFILFLVITALMYLYYEGKFAIQKLGGFVF
                     GFMAVVVGFVLWYSVSREAHTIQPLIPALQSWWMKIHVPANFIGYGAFCISAMLGIAE
                     LVSLRAEGKGGKLWLPPSALIDEVMYKAIAVGFLFFTIATILGALWAADAWGRYWSWD
                     PKETWAFIVWLNYAVWLHLRLVAGWRGKVLAWWAIIGLFVTAFAFIGVNMFLSGLHSY
                     GTL"
     gene            complement(4867..6819)
                     /locus_tag="WIC03_000385"
     CDS             complement(4867..6819)
                     /locus_tag="WIC03_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein"
                     /protein_id="HEZ3882941.1"
                     /translation="MRFAVALLSLLGIASVIGTVLQQNQPQTDYLVKFGSFWAQIFGF
                     LGLYDVYASAWFVVIMMFLVVSTSLCLIRNVPPFWREMKSFREKVKEKSLAAMRHSSL
                     LDVKIAPEVAKRYLEVQGFQGKTINREDGSVLIAAKKGTMNKWGYIFAHVALIVICLG
                     GLIDSNLLLKLGMLTGRIVPDNQAVYAKDFKPESILGASNLSFRGNVNISEGQSADVV
                     FLNADNGILVQDLPFEVKLKKFHIDFYNTGMPRDFASDIEVTDKATGEKLERTIRVNH
                     PLTLHGITIYQASFADGGSDLTFKAWNLGDASREPVVLKATSIHQFPLEIGKHKYRLE
                     FDQFTSMNVEDMSEGAEREKSLKSTLNDVRAVTQEGKKYTNIGPSIVYRIRDAAGQAV
                     EYKNYMLPVLQEQDYFWITGTRSGLQQQYRWLRIPLDKQLKADTFMALREFLKDGKGR
                     KRLVADATKDAPAEIREQFMLAAENTLNIFAQKGYLGLDEFITSNIPKEQQDKMQGYF
                     YEMLYGVMNAALDETIRRYGLPEWQQDEARNRFLLHSMDAYTGLTEYPAPMLLQLDGF
                     SEVRSSGLQMTRSPGALLVYLGSVLLVLGTVLMFYVREKRAWVLFSDGKIRFAMSSAR
                     SERDLQKEFPKHVESLQRLGKDLNHD"
     gene            complement(7096..7719)
                     /locus_tag="WIC03_000386"
     CDS             complement(7096..7719)
                     /locus_tag="WIC03_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c4"
                     /protein_id="HEZ3882942.1"
                     /translation="MKRLTLLAFVLAAGAVSASPKADVEKGKQVAATVCAACHAADGN
                     SGIAMYPRLAAQHTAYIYHQTIGIRDGKRTHGSAAVMKPVVMNLSDQDILNVSAFYAK
                     QQPKSGEANPKENPELGAKIYRGGLSDKKVPACMSCHGPSGAGMPGGGSEIQAYPRLG
                     GQHQAYIVEQMNAYKSGQRKNTIMEDIANRMSEEDLKAVANFIQGLR"
     gene            7925..8554
                     /locus_tag="WIC03_000387"
     CDS             7925..8554
                     /locus_tag="WIC03_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEZ3882943.1"
                     /translation="MNLFQNAKFFTTVNHLKDLPDTPLEIAFVGRSNAGKSSAINTLT
                     NHVRLAYVSKTPGRTQHINFFELQNGNFMVDLPGYGYAQVPEAVRAHWVNLLGDYLQQ
                     RKQLIGLVLIMDARHPLKELDIRMLDFFHTTGRPVHILLSKADKLSKNEQIKTLSQVK
                     KLLKPYSDRQNISVQLFSSLKKQGIDEANRTVGSWFDAADAAASSPKEN"
     gene            complement(8675..11071)
                     /locus_tag="WIC03_000388"
     CDS             complement(8675..11071)
                     /locus_tag="WIC03_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HEZ3882944.1"
                     /translation="MIKKILTTCFGLVFGFCVFGVGLVAIAILVTYPKLPSLDSLQHY
                     QPKMPLTIYSADGEVIGMYGEQRREFTKIGDFPEVLRNAVIAAEDKRFYRHWGVDVWG
                     VARAAVGNVVSGSVQSGASTITQQVAKNFYLSSEKTFTRKFNEVLLAYKIEQSLSKDK
                     ILELYFNQIYLGQRAYGFASAAQIYFNKNVRDLTLAEAAMLAGLPKAPSAYNPIVNPE
                     RAKLRQKYILNNMLEEKMITVQQRDQALNEELHYERFVRKIDQSALYVAEMVRQELYE
                     KYGEDAYTQGFKVYTTVRADHQKVATEALRKALRNFDRGSSYRGAENYIDLSKSEDVE
                     ETVSQYLSGLYTVDKMVPAVVLDVTKKKNVVIQLPGGRRVTLDRRALGFAARAVNNEK
                     MGEDRIRRGAVIRVKNNGGRWAVVQEPLLQGALVSLDAKTGAVRALVGGYDFHSKTFN
                     RAVQAMRQPGSTFKPFVYSAALSKGMTASTVVNDAPISLPGKGPNGSVWTPKNSDGRY
                     SGYITLRQALTASKNMVSIRILMSIGVGYAQQYIRRFGFRSSELPASLSMALGTGETT
                     PLKVAEAYSVFANGGYRVSSHVIDKIYDRDGRLRAQMQPLVAGQNAPQAIDPRNAYIM
                     YKIMQDVVRVGTARGAAALGRTDIAGKTGTTNDNKDAWFVGFNPDVVTAVYIGFDKPK
                     SMGRVGYGGTIAVPVWVDYMRFALKGKQGKGMKMPEGVVSSNGEYYMKERMVTDPGLT
                     LDNSGIAPQPSRRAKEDDGGAAEGGRQAADDEVRQDMQETPVLPSNTGSKQQQLDSLF
                     "
     gene            11223..12338
                     /locus_tag="WIC03_000389"
     CDS             11223..12338
                     /locus_tag="WIC03_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HEZ3882945.1"
                     /translation="MRLFKSLKNPKKTDAKLPKKSSGLNNRAAIGIDIDQHSIKMVQL
                     SGRSLNQIQLEKYVIAKLPKNIIQGNKVQNYDQLVTYLQQAYAKLGTSCKNIIASVPQ
                     NLATIEQLTYTDKDAELDLQGFVESSISEVSSISLEEANYDYQVLSQSAAGEAVLAVA
                     SRKDEIEPLIDAFNAAGMKLSALDVDIFGQYNAYALWINHFAPELAAEKVAIFGVYAA
                     QTYALVIQDGKILYKQETSVSEEQLNQLIQRTYQVTEEKAEEIINSPQKPSDYQESVA
                     NYFNQQITQEIQRVLQFYYTTQTADDMTDIKHILLTGEAARQEGIAQTVASQTNADVQ
                     CVHPARYFADNLKTDKQQFELDAPTLTRAFGLAVRGL"
     gene            12341..12940
                     /locus_tag="WIC03_000390"
     CDS             12341..12940
                     /locus_tag="WIC03_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEZ3882946.1"
                     /translation="MNNLIKINLLPYREEMNKRKQQQFKTLMYGAVLTGVAAVAATYL
                     FIDNMINNQSERNTLLETSIAHLDTELSEIQKLKQEKDAFLIKKNKIEELQLKRLQAA
                     KILDSLNEAVPGSTYLTSLDAVTADSYRLSGRTSSDNRVAAMMRAMPNTGIFKQPELL
                     SIKKNNSHQEFTLQATLQPIVKAAESKENPASGNAQEAN"
     gene            12941..13588
                     /locus_tag="WIC03_000391"
     CDS             12941..13588
                     /locus_tag="WIC03_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEZ3882947.1"
                     /translation="MASKSSKTNLDLNNLHLLNLPARLFIALLAVAAVLGLGYAGLFK
                     SQMESLEEYEAKETELKNTYKQKSIDAASLNNLRDELASIRSAFDIMLKQLPTDAEIP
                     NLVQELHQAGSSNGLRLDSVMPQPPVDDGPIKRLPYSISITGNYEQISQFTRDVGSLS
                     RIITLESLKIAQSPENGGNPDGKSSILNLSAIATTYQAKSVEELAAEAAQNAEQK"
     gene            13606..14151
                     /locus_tag="WIC03_000392"
     CDS             13606..14151
                     /locus_tag="WIC03_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HEZ3882948.1"
                     /translation="MKHYALLISFLALSACSQGSEDLNEWMAQTRREAKAEIIPFQAP
                     TLPVAPVYSPPQLTGPNAFDFRRMETDKKGENAPDTKRIKETLEKFSLENMRYVGILK
                     SGQKVSGFIEAEGYVYTVGVGNYLGQNYGRIESITDDSIVLNELIEDSTGNWVSRKAE
                     LLLNSSDKNTEQAAAPAAEQN"
     gene            14170..16455
                     /locus_tag="WIC03_000393"
     CDS             14170..16455
                     /locus_tag="WIC03_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HEZ3882949.1"
                     /translation="MNTKLTKIISGLFVATAAFQTASAGNITDIKVSSLPNKQKIVKV
                     SFDKEIVNPTGFVTSSPARIALDFEQTGISMDQQVLEYADPLLSKISAAQNSSRARLV
                     LNLNKPGQYNTEVRGNKVWIFINESDDTVSAPARPAVKAAPAAPAKQQATAPSTKSAV
                     SVSKPFTPAKQQAAAPFTESVVSVSAPFSPAKQQAAASAKQQTAAPAKQQAAAPAKQT
                     NIDFRKDGKNAGIIELAALGFAGQPDISQQHDHIIVTLKNHTLPTTLQRSLDVADFKT
                     PVQKVTLKRLNNDTQLIITTAGNWELVNKSAAPGYFTFQVLPKKQNLESGGVNNAPKT
                     FTGRKISLDFQDVEIRTILQILAKESGMNIVASDSVNGKMTLSLKDVPWDQALDLVMQ
                     ARNLDMRQQGNIVNIAPRDELLAKDKAFLQAEKDIADLGALYSQNFQLKYKNVEEFRS
                     ILRLDNADTTGNRNTLVSGRGSVLIDPATNTLIVTDTRSVIEKFRKLIDELDVPAQQV
                     MIEARIVEAADGFSRDLGVKFGATGKKKLKNDTSAFGWGVNSGFGGDDKWEAETKINL
                     PITAAANSISLVRAISSGALNLELSASESLSKTKTLANPRVLTQNRKEAKIESGYEIP
                     FTVTSIANGGSSTNTELKKAVLGLTVTPNITPDGQIIMTVKINKDSPAQCASGNQTIL
                     CISTKNLNTQAMVENGGTLIVGGIYEEDNGNTLTKVPLLGDIPVIGNLFKTRGKKTDR
                     RELLIFITPRIMGTAGNSLRY"
BASE COUNT         4529 a         4753 c         3806 g         3637 t
ORIGIN      
        1 aaaatgccgt ctgaactgtt cagacggcat ttttgatttt caaaccggaa ttacagcccc
       61 gctgccgccc tcaatgcagc agctttgtcg gtgcgctccc aagtgaactc aggttcttcg
      121 cggccgaaat gtccgtaagc ggcggattta ctgtaaatcg ggcgcaagag atcgagcatt
      181 tggacgatgc ctttggggcg caggtcgaaa tgttcgcgaa ctaaggtaat cagtttttcc
      241 tcgctgattt tgcctgtgcc gaaagtgtcg atggaaatcg aagtcggttc ggcaacgccg
      301 atggcgtagg aaacttggat ttggcattgg gttgccaatc ctgcggcgac gatgtttttc
      361 gccacatagc ggcaggcgta ggcggcggaa cggtcaacct tggacgggtc tttgccggaa
      421 aacgcgccgc cgccgtgcgg agccgcgccg ccgtaggtat cgacgatgat tttacggccg
      481 gtcaaaccgc agtcgccttg cgggccgccg atgacgaagc ggccggtcgg gttgatcagg
      541 tatttggttt cgtcggtcag cagttcagac ggcaggacgg gcttgataat ctgctcgatg
      601 acggcgtttt tcagttcttc gtaagcgatg gacggatcgt gctgggtaga gaggacgacg
      661 gtatcaatgc gttttacctt accggtttcg ctgtcgtaaa ccacggtcaa ctgcgctttt
      721 gcgtcgggac gcagccacgg caggcggccg tctttacgca attcgctttg gcgttgcatc
      781 aggcggtggc tgtaatagat ggcaaacggc atcagggtcg gggtttcgtc gcaggcataa
      841 ccgaacatca aaccttggtc gcctgcgcct tggttcaagt cgataccttc gccttcgttt
      901 acgccttggg cgatgtcggg ggattgttgg tcgtagtaca cgccgaccgc gcagccgttg
      961 gcatcaaagc ccagctcgga ggagttgtag ccgatgcgtt tgatggtttc gcgtgcgact
     1021 ttgatgtagt ccacttgggc ggtggtggtg atttcgcctg ccaatacgca caagcctgtg
     1081 ttgaccaaag tttctgcggc gacacgcgct ttggggtctt gcgccaagat ggcatccaaa
     1141 atcgcatcgg atacttggtc ggcaactttg tccggatggc cttcggatac cgattcggaa
     1201 gtaaacagat attcgctcat tgagattttc cttgaaaaac aaaccatctt cttcagacgg
     1261 catgttgtat gaacataatg tcgacagcgg gaaatatagc aaaatttccc tattcatacc
     1321 attcagttga gaaatattcc catttgaatg gcactttgga atctctgccc gtacgtttct
     1381 tacaggcaaa aaaattcccg catcaagcgg gtttggattg ctctggtgag ccacatcggc
     1441 ttttcaaccg tccaccttac ttttcctttt gaaaagcagg ttggcatgga attcccaact
     1501 cttaatgcag cgcggatggt agcagaaaag cctttccgcc gcaatacttc cctttttcag
     1561 acggcatgtt tcgtttacaa ttcgggctgt ttcccccttt gcgaaccgcc atgcacatcc
     1621 tgttgaccgc cctgctcaaa tgcctctccc tgctgccact ttcctgtctg cacacgctgg
     1681 gaaaccggct cggacatctg gcgttttacc ttttaaagga agaccgcgcg cgcatcgtcg
     1741 ccaatatgcg tcaggcaggc atgaatcccg accccaaaac agtcaaagcc gtttttgcgg
     1801 aaacggcaaa aggcggtttg gaacttgccc ccgcgttttt cagaaaaccg gaagacatag
     1861 aaacaatgtt caaagcggta cacggctggg aacatgtgca gcaggctttg gacaaacacg
     1921 aagggctgct attcatcacg ccgcacatcg gcagctacga tttgggcgga cgctacatca
     1981 gccagcagct tccgttcccg ctgaccgcca tgtacaaacc gccgaaaatc aaagcgatag
     2041 acaaaatcat gcaggcgggc agggttcgcg gcaaaggaaa aaccgcgcct accagcatac
     2101 aaggggtcaa acaaatcatc aaagccctgc gttcgggcga agcaaccatc gtcctgcccg
     2161 accacgtccc ctcccctcaa gaaggcgggg aaggcgtatg ggtggatttc ttcggcaaac
     2221 ctgcctatac catgacgctg gcggcaaaat tggcacacgt caaaggcgtg aaaaccctgt
     2281 ttttctgctg cgaacgcctg cctggcggac aaggtttcga tttgcacatc cgccccgtcc
     2341 aaggggaatt gaacggcgac aaagcccatg atgccgccgt gttcaaccgc aatgccgaat
     2401 attggatacg ccgttttccg acgcagtatc tgtttatgta caaccgctac aaaatgccgt
     2461 aacgaaaata aaaatgccgt ctgaacaatt tcagacggca ttttgtcatc tgacgatttc
     2521 cgacagcggc cagcgcggac ggacattgaa cgcaccgacc tccctgccct tgcccaggtg
     2581 catcgcaccg gcaaaggcaa tcatggcacc gttgtccgtg cagtatgccg tcggcgggaa
     2641 aaacacgctg actttttcgg acggatgttt cggcttgcct tttggggtcg ggatttgcac
     2701 cgtcatattg ccgaaagttt cacggagttt gcggtttgca ccgaccccgc cggcgaccac
     2761 tacggttctg aaccctgtct gcaacagggc ttttttcact ttcgccgcca acacatcgac
     2821 taccgcatct tgaaacgcac ggcaaatatc gttgcgggtt tgctcgggaa tggtttccga
     2881 accgtttgcc tcacgcactt tttcaacggc agtcaaaaca gcggttttca aacctgaaaa
     2941 actcatctgc aaatcgtcgg aatgaatcat cgggcgcgga aaaacaaacg cttcgggcct
     3001 gcccgattcc gcaagttccg acagtttcgc accgcccgga tacggcaagc ccagcagttt
     3061 cgccgttttg tcgaatgcct cgcccgccgc atcatcgacg ctctcgccca aaagcgcgta
     3121 gtcgcctatg cccctgaccg ccataatctg cgtatgcccg cccgaaacca gcagcgcgac
     3181 aaaaggaaag tcgggttttt cctccgccaa cagcggcgac agcagatgtc cttccaaatg
     3241 atggacggga ataacaggct tgtccaacgc taaagccagc gcgttggcgt agctcgaacc
     3301 cgccagcagc gcaccgccca aaccgggccc ctgcgtaaag gcaaccgcgt caatgtcgcc
     3361 atacgatgcg cccgcctgcg ccaaacagcc ttcagtcaac ggaacaaggc ggcggatatg
     3421 gtcgcggctt gccaattccg gcacaacccc gccgtattcg gcgtgcattg ccatttgagt
     3481 gtgcaggcag tgcgcccgca atccacgttc cgtatcgtaa agcgcaacac ctgtttcgtc
     3541 gcaagaagac tcgattccta ataccaacat ggtctgatgc cgttaaaaac tgaaaaacgt
     3601 attttagcgg atttcggcac gactgccgta tcccaaaaac ggaacatgcc gtctgaagac
     3661 cgttcagacg gcatcgtcgc accgtatcaa agcgttccgt aagaatgcag cccgctcaaa
     3721 aacatattca cgccgataaa ggcaaatgcg gttacgaaca aaccgataat cgcccaccac
     3781 gccagcactt tgccgcgcca accggcaacc agccgcaagt gcagccaaac ggcgtaattg
     3841 agccagacga tgaacgccca cgtctctttc ggatcccaac tccaatagcg tccccaagca
     3901 tctgccgccc acagcgcacc caaaatggtg gcaatagtaa agaacagaaa gccgacggca
     3961 atcgccttat acatcacctc gtcgatcaat gccgacggcg gcagccacag ttttccgcct
     4021 tttccttccg cacgcaggga aaccagttcg gcaataccga gcatcgcgga aatgcaaaac
     4081 gcgccgtaac cgataaagtt tgccggaacg tggattttca tccaccagga ctggagcgcg
     4141 ggaatcagcg gctggatggt atgcgcctcg cgggacacgc tgtaccacaa gacaaatcca
     4201 accacgaccg ccataaagcc gaacacgaag ccgcccaatt tctgtatggc gaacttacct
     4261 tcataataaa gatacatcag cgcggtaatc accaaaaaca ggatgaacac ttcatacagg
     4321 ttggaaaccg gaatatgccc cgcatcggga cggagcagat agctttcgtg ccaacgtacc
     4381 agcagaccgg taaagcctgc tacggcagac acccatgcaa acacggttcc catacccaac
     4441 agcgtgttgg tcggcacatt ttttacgctt gccaaaaccg cgcccgaaat ataagcgaac
     4501 agggcgaaaa agacaaaggc gcactgccac atgatcgccg attggctgct gaggaaatac
     4561 cgcaacagga aaatctctgc cgatttgatg ttgccgccat acagaccgac ggcggcatag
     4621 gcaagcaata cgcttaaagg aacaaaccag cgcatcggtt tgaaaaacca acccaaaaac
     4681 acggcaatac cggcactcgc ccacaacatg accgtttcgt aaatgtccat atgcataccg
     4741 gaacgggtct gtacgaaaac cgtagccgca aaaaccagca cggcaaatac ccaatcccaa
     4801 agattcagat tgctgatcaa agacttctga atcagcagct cgtgttccgg aagggtttta
     4861 tagtgttcag tcatgattca agtccttgcc gagccgttgc agactctcga cgtgttttgg
     4921 aaattccttc tgcaaatccc gttcactgcg ggccgaagac atggcaaaac ggattttgcc
     4981 gtctgaaaac aatacccacg cccgtttttc gcgcacataa aacatcaata ccgtacccaa
     5041 taccaacagc accgagccga gatagaccaa aagcgcaccc ggggaacggg tcatctgcaa
     5101 acccgacgaa cgcacctcgg aaaacccatc aagttgcagc agcataggcg cgggatattc
     5161 ggtcaaaccc gtgtacgcat ccatactgtg cagcaggaaa cggttccgcg cttcatcctg
     5221 ctgccattcg ggcaagccgt accggcgtat ggtttcatcc aaagcagcgt tcatcacgcc
     5281 gtaaagcatt tcgtagaaat agccctgcat cttatcctgc tgctctttcg ggatattgga
     5341 cgtaataaat tcgtccaatc ccaaatagcc tttttgtgca aagatgttca gcgtgttttc
     5401 cgcagccagc atgaattgtt cgcggatttc ggcaggtgcg tctttggttg cgtcggcaac
     5461 cagacgtttg cgccccttcc cgtctttcag aaactcgcgc aacgccataa aggtgtccgc
     5521 tttcaactgc ttgtccaagg ggatacgcag ccagcggtat tgctgctgca agccgctgcg
     5581 cgtgccggta atccaaaaat aatcctgttc ctgcaaaacc ggcagcatat agtttttata
     5641 ttcgaccgcc tgccctgccg catcacggat acggtaaaca atggaagggc cgatattggt
     5701 gtatttttta ccttcctgag taacggcgcg gacatcgttc agcgtggatt tcaggctttt
     5761 ttcccgttcc gcgccctcgc tcatgtcctc cacattcata gaagtgaact gatcgaactc
     5821 aagacgatat ttgtgtttgc caatttccaa cggaaactgg tgtatggatg ttgccttcaa
     5881 cacgacaggc tcgcgcgaag catcacccaa attccacgcc ttgaatgtca aatccgaacc
     5941 gccgtcggca aaactcgcct gataaatcgt gatgccgtgc aaggtcaaag gatggttcac
     6001 gcggatggtg cgctcgagtt tctcaccggt tgccttgtcc gtcacttcaa tatcactggc
     6061 aaaatcgcgc ggcataccgg tattgtaaaa gtcgatatga aattttttca gtttgacttc
     6121 aaaaggcaag tcctgaacca atatcccgtt gtcggcattc aggaaaacca catccgcact
     6181 ctgcccctcg gaaatattga cgttgcccct aaatgagaga ttggacgcac ccaaaatact
     6241 ttcgggcttg aaatccttgg cataaaccgc ctgattgtcc ggaacaatcc gaccggtcag
     6301 catacccagt ttcaacagca ggttactgtc tatcaacccg cccaggcaaa tgacaatcaa
     6361 agcaacatgg gcaaagatat agccccattt gttcattgtg ccttttttgg cggcaatcag
     6421 aaccgacccg tcttcacggt taatggtttt tccctgaaaa ccttgtactt ccagataacg
     6481 tttggcaacc tcgggcgcaa tttttacatc caacagcgaa gaatggcgca tcgccgccag
     6541 agatttttct ttaacctttt cccgaaaaga cttcatttcg cgccagaacg gcggcacatt
     6601 gcgaatcagg cacaaactgg tagaaaccac caaaaacatc atgataacga caaaccatgc
     6661 cgaagcatag acgtcataca gtcccagaaa accaaaaatc tgcgcccaaa acgatccgaa
     6721 tttgaccaaa taatccgtct gcggctggtt ttgctgcaac accgtaccga taaccgatgc
     6781 aatacccagc agactgagca aagcgactgc aaagcgcatg gagctgaaaa aagcgaacca
     6841 cggacgggaa agaagtgggg gagatctacg ggatttactc attgtgtgtt tattccgcca
     6901 tcaggaatat gggaaagcag aattgggcaa acagaaaaca acgtcccgat tctactgtct
     6961 tgatgctttt tatttcaaga caatgaagac agcctgcatc gattccaacg gttgcgattg
     7021 aaaaaactta tcgcagaatt gcctgaagcc gtctgaaaac ttttcagacg gcctctaaaa
     7081 cagactattg cggaattaac gcaaaccttg gataaagttg gcgaccgctt tcaaatcttc
     7141 ttcagacata cggtttgcaa tatcttccat gatggtattt ttacgctgac cggacttgta
     7201 ggcattcatc tgttcaacaa tatatgcctg atgctgaccg cccaaacgcg gataagcctg
     7261 aatttcgctt ccgcctcccg gcatacccgc accgctcgga ccgtggcagg acatacacgc
     7321 cggcactttt ttatcgctca aaccgccgcg atagattttc gcacccaatt cgggattttc
     7381 cttaggattg gcttcaccgg atttgggctg ctgtttggca tagaatgcgg atacgttcaa
     7441 aatatcctga tcgctcaaat tcattaccac cggtttcatc acagctgccg aaccgtgggt
     7501 gcgtttaccg tcgcggatgc cgatagtttg atgatagatg taagcagtat gctgtgccgc
     7561 caaacgcgga tacatcgcaa tgccgctgtt accgtctgct gcatggcaag ccgcacaaac
     7621 cgttgcggca acctgtttgc ctttttccac gtctgctttg ggagaggcgg aaaccgcacc
     7681 ggcagccaaa acaaaggcca ataaagtcaa tcgtttcatg gagtgctcct gattacagca
     7741 ttggataacg caacaatgct ctttttatat tcaaatacgg gatttttgac ccgattaaaa
     7801 ccgatgattc tgtaaacgtg ttattctata ctaaatttac attaaattac cactgtgttt
     7861 cacataaaac caaccgcata tttttgctgt cggacaaacg gcggcggaaa acaaggatat
     7921 gcccatgaac ctttttcaaa acgccaaatt cttcacgacg gtcaaccacc ttaaagacct
     7981 gcccgacacc cctctcgaaa ttgcctttgt cggcaggagc aatgccggaa aatccagtgc
     8041 catcaatacc ctgaccaacc atgtccgtct tgcctacgtt tcaaaaacac ccggacggac
     8101 gcagcatatc aacttcttcg agctgcagaa cggcaatttt atggtcgatt tgcccggcta
     8161 cggttatgcc caagtccccg aagcagtacg cgcacattgg gtcaatctgc tcggcgacta
     8221 tctgcaacag cgcaaacagc ttatcgggct ggttttgatt atggatgccc gccatccttt
     8281 aaaagaactc gacatccgta tgctggactt tttccacacg accggcagac cggttcacat
     8341 cctgctgtca aaagccgaca aattatccaa aaacgaacag ataaaaaccc tgtcccaagt
     8401 caaaaaactg ctcaaacctt attccgacag gcaaaacatc agcgtacagc tgttttccag
     8461 cctgaaaaaa caaggtattg acgaggccaa ccgaactgtc ggaagctggt ttgacgcagc
     8521 agatgccgcc gcttcctctc caaaggaaaa ctgaccccaa ttatacggaa accgtatttc
     8581 ccccacttga ccgaccgcaa acatttaaaa aattgccact gccaaatcta aaatgccgtc
     8641 tgaaagactt ttcagacggc attttgcgga gtctttaaaa cagagaatcc aactgctgct
     8701 gtttggaacc agtattactc ggaagcaccg gcgtttcctg catatcttgg cggacttcgt
     8761 catccgccgc ctgccgtccg ccttctgccg cgcccccgtc atcttctttt gcccgtcggg
     8821 aaggttgcgg cgcaataccg ctgttgtcca gcgtcaagcc cggatcggtt accatacgtt
     8881 ccttcatata gtattcgcca ttgctgctga ccacaccttc aggcattttc atccccttgc
     8941 cctgctttcc tttcaacgca aaacgcatat agtccaccca aaccggcacc gcaatcgtac
     9001 cgccgtagcc gacacgcccc atactcttag gtttgtcgaa gccgatatat acggcagtaa
     9061 ccacatcagg gttaaaaccg acaaaccacg catccttatt gtcattggtc gtacccgttt
     9121 taccggcaat atccgttctt cccaacgcag ctgcccccct tgccgtacca acacggacca
     9181 catcctgcat aatcttatac ataatatagg cattgcgcgg atcgattgcc tgaggcgcat
     9241 tttgcccggc caccaaaggt tgcatttggg cgcgcaacct gccgtctctg tcataaatct
     9301 tatcgattac gtgcgaagaa accctatatc cgccgttcgc aaatacgcta tatgcctccg
     9361 ccactttcaa cggcgttgtc tcgcccgtac ctaaagccat agacaggctt gccggcagct
     9421 cggacgacct gaagccgaaa cgccggatat actgttgcgc gtaaccgaca ccgatagaca
     9481 tcaaaatacg gatggaaacc atattcttgg aagccgtcag agcctgtctc aaagtaatgt
     9541 agccggaata tctgccgtct gaatttttag gtgtccagac cgaaccgttc ggccctttcc
     9601 ccggcaggga aatcggcgca tcgttaacca ctgtggacgc ggtcatcccc ttagataatg
     9661 ccgccgaata gacaaacggc ttaaaggtcg aacccggctg ccgcattgcc tgaacggcac
     9721 gattgaatgt tttgctgtga aaatcataac cgccgaccag cgcgcgcaca gctccggttt
     9781 ttgcatccag cgaaaccaaa gccccctgca gcaacggctc ttgaaccacc gcccaacgcc
     9841 cgccgttgtt tttgacacgg atgaccgcgc ccctgcggat acggtcctcc cccatttttt
     9901 cattattgac cgcgcgggcc gcaaagccca aggcgcgcct atcaagcgta acccgcctgc
     9961 cgccgggcag ctgtatgacg acatttttct ttttagtcac atccaacaca acggcgggaa
    10021 ccattttatc gacggtatag agtcccgaca gatactggct gacagtctcc tcgacatctt
    10081 cactcttact caaatcgata tagttttccg caccgcggta gctgctgccg cgatcgaaat
    10141 tccgtagagc cttgcgcaat gcctcggttg ccaccttctg atgatcggcg cggaccgtgg
    10201 tataaacctt aaaaccctgc gtataggcat cttcaccgta tttctcatac agttcctgac
    10261 gcaccatttc cgccacatat aaagcactct gatcgatttt ccgaacaaac cgctcgtaat
    10321 gcagttcctc attcaacgcc tgatcgcgct gttgcacggt aatcatcttc tcctcgagca
    10381 tattgttcaa aatatacttc tggcgcaact tggcacgttc cggattaaca atcggattat
    10441 aggcagacgg agccttgggc agtcccgcaa gcatggcggc ttccgccaaa gtcaaatctc
    10501 ggacattctt attgaaatag atttgcgcgg cagatgcaaa accataggcg cgctgaccga
    10561 ggtaaatctg attgaaatac aactcgagga ttttgtcttt gcttaaagac tgctcgattt
    10621 tataggcaag caacacctca ttgaatttgc gtgtgaacgt tttttcactg ctcaaataaa
    10681 aatttttcgc cacctgctgc gtaatcgtac tcgcacccga ctgcacgctg ccggacacga
    10741 cattgccgac ggcagcgcgg gcaacacccc aaacatccac cccccaatgc cggtaaaagc
    10801 gtttatcctc ggcggcgata accgcattcc gcaacacctc tgggaaatcg ccgatttttg
    10861 taaattcgcg ccgctgctcc ccatacatac cgatgacttc cccatccgcc gaataaatag
    10921 tcaacggcat tttaggctgg taatgctgca aagaatccaa agacggcagt ttcggatacg
    10981 ttaccaaaat agcaatggca accaaaccca ctccaaatac acaaaaccca aaaaccaaac
    11041 caaaacaagt cgttaaaatc tttttaatca tagctgaata ataatttacc attattggta
    11101 ttaaataaag taaaatagca accgatttct acaaagcacg gtttcaatgt gcaaagaaca
    11161 aggaatccat tacggatacc gaaacggtta ctcactgtac aaataaagca ggaactttca
    11221 tcatgcgctt gtttaaaagc ttgaaaaacc ctaaaaaaac agatgccaag ctccctaaaa
    11281 aatcttcggg actcaataac cgcgcggcaa tcggcatcga tatcgaccag cattccatca
    11341 aaatggtcca attgtcagga cgtagtttaa accaaattca attggaaaaa tacgtcattg
    11401 ccaaattacc aaagaatatc attcaaggca ataaagtcca aaattacgat caacttgtta
    11461 catatttgca acaagcctat gccaaactgg gtacttcgtg caaaaacatc atcgcgtccg
    11521 tcccgcaaaa tctggcaacc atcgaacaat tgacctacac agacaaagat gcagaattag
    11581 acctgcaggg gttcgtggag tcctccatct ccgaagtcag ctcgatatcg ctcgaagaag
    11641 ccaattacga ctatcaggtc ttgtcccaat cggccgccgg cgaagctgtg ttggccgtcg
    11701 catcgagaaa ggatgaaatc gaacccctga ttgacgcatt caacgccgcc ggtatgaaat
    11761 tatccgcgct tgatgtggac attttcggac aatacaacgc ctacgcgcta tggataaacc
    11821 atttcgcccc cgagcttgca gccgaaaaag tcgccatttt cggcgtatat gccgcacaga
    11881 cctacgcctt ggtcatccaa gacggaaaaa tcctatacaa acaggaaacc tccgtcagcg
    11941 aagaacagct caaccaactc atccagcgca cctatcaggt aacagaagaa aaagcggaag
    12001 aaatcatcaa ctccccgcaa aaaccttccg attaccaaga aagcgtggca aactatttca
    12061 accagcagat tacccaagaa atacaaaggg tcttgcagtt ttattacacc acgcagaccg
    12121 cagacgatat gaccgacatc aagcatatcc tgctgaccgg ggaagcggcg cgccaggaag
    12181 gtatcgccca aaccgtcgcc tcacaaacca atgcagatgt acaatgcgtc catcccgcgc
    12241 gttattttgc ggacaacctc aaaacagaca aacaacaatt cgaacttgat gcgccgacac
    12301 tgaccagggc gttcggtttg gcggtacggg gattataatt atgaacaatt taatcaaaat
    12361 caacctcctc ccctacaggg aagagatgaa caagcgcaaa cagcagcagt ttaaaacgct
    12421 gatgtacggt gccgtgctga cgggcgttgc cgccgttgcg gcaacctacc tgtttatcga
    12481 caatatgatc aataaccagt cggaaagaaa cacgctgctg gaaacctcca tcgcacactt
    12541 ggataccgag ctgtcggaaa tacaaaagct caaacaggaa aaagatgcct tcctgattaa
    12601 gaaaaacaaa atcgaggagc tccagctcaa acgcctccaa gccgcaaaaa tcctcgacag
    12661 cctgaatgag gccgtccccg gaagcaccta cctgacctcg ctggatgccg ttaccgccga
    12721 ctcttatcgg ctcagcggca ggacatccag cgacaaccgc gttgccgcca tgatgagggc
    12781 gatgcccaat accggcatat tcaagcaacc cgaattgtta agcatcaaga aaaacaattc
    12841 gcatcaagaa tttacccttc aggcaacatt acaacccatc gtaaaggcgg ccgaatccaa
    12901 agagaatccg gcttcgggaa acgcacagga ggcaaactga atggcttcta aatcatctaa
    12961 aaccaacttg gatctcaaca accttcacct gctcaacctt cctgccaggc tttttatcgc
    13021 cctgctggcc gttgccgccg tgctggggct cggttatgcc ggattgttca aaagccagat
    13081 ggaatccctt gaggaatacg aagcaaaaga aaccgaactg aaaaacacct acaaacagaa
    13141 aagtatcgac gcggccagcc tgaacaacct gagggacgaa cttgcctcaa tccgctctgc
    13201 cttcgatatc atgttgaaac agctgccgac agatgcagaa attcccaacc tggttcaaga
    13261 gcttcatcag gcaggttcga gcaacggcct gcgcttggac agcgttatgc cccaacctcc
    13321 cgtagatgac ggccccatca aaagattacc ctattccatt tccattaccg gaaattacga
    13381 acagatcagc caatttaccc gcgatgtcgg cagcctctcc cgaatcatta cccttgagtc
    13441 gctgaaaatc gcccaatctc cggaaaacgg cggcaatcct gacggcaaga gcagcatcct
    13501 gaacctcagt gccattgcca ccacctacca agcaaaatcc gtagaagagc ttgccgcaga
    13561 agcggcacaa aatgccgagc aaaaataact tacgttaggg aaaccatgaa acactatgcc
    13621 ttactcatca gctttctggc tctctccgcg tgttcccaag gttctgagga cctaaacgaa
    13681 tggatggcac aaacgcgacg cgaagccaaa gcagaaatca tacctttcca agcacctacc
    13741 ctgccggttg cgccggtata cagcccgccg cagcttacag ggccgaacgc attcgacttc
    13801 cgccgcatgg aaaccgacaa aaaaggggaa aatgcccccg acaccaagcg tattaaagaa
    13861 acgctggaaa aattcagttt ggaaaatatg cgttatgtcg gcattttgaa gtctggacag
    13921 aaagtctccg gcttcatcga ggctgaaggt tatgtctaca ctgtcggtgt cggcaactat
    13981 ttgggacaaa actacggtag aatcgaaagc attaccgacg acagcatcgt cctgaacgag
    14041 ctgatagaag acagcacggg caactgggtt tcccgtaaag cagaactgct gttgaattct
    14101 tccgacaaaa acaccgaaca agcggcagca cctgccgcag aacaaaatta agaagaggat
    14161 tactccatta tgaataccaa actgacaaaa atcatttccg gtctctttgt cgcaaccgcc
    14221 gcctttcaga cagcatcggc aggaaacatt acagacatca aagtttcctc cctgcccaac
    14281 aaacagaaaa tcgtcaaagt cagctttgac aaagagattg tcaacccgac cggcttcgta
    14341 acctcctcac cggcccgcat cgccttggac tttgaacaaa ccggcatttc catggatcaa
    14401 caggtactcg aatatgccga tcccctgttg agcaaaatca gtgccgcaca aaacagcagc
    14461 cgtgcgcgtc tggttctgaa tctgaacaaa ccgggccaat acaataccga agtacgcggg
    14521 aacaaagttt ggatattcat taacgaatcg gacgataccg tgtccgcccc cgcacgcccc
    14581 gccgtaaaag ccgcgcctgc cgcaccggca aaacaacagg ctaccgcacc gtctaccaag
    14641 tccgcagtat ccgtatccaa accctttacc ccggcaaaac aacaggctgc cgcaccgttt
    14701 accgagtccg tagtatccgt atccgcaccg ttcagcccgg caaaacaaca ggcggcggca
    14761 tcagcaaaac aacagacggc agcaccagca aaacaacagg cggcagcacc agcaaaacaa
    14821 accaatatcg atttccgcaa agacggcaaa aatgccggca ttatcgaatt ggctgcattg
    14881 ggctttgccg ggcagcccga catcagccaa cagcacgacc acatcatcgt tacgctgaaa
    14941 aaccataccc tgccgaccac gctccaacgc agtttggatg tggcagactt taaaacaccg
    15001 gttcaaaagg ttacgctgaa acgcctcaat aacgacaccc agctgattat cacaacagcc
    15061 ggcaactggg aactcgtcaa caaatccgcc gcgcccggat actttacctt ccaagtcctg
    15121 ccgaaaaaac aaaacctcga gtcaggcggc gtgaacaatg cgcccaaaac cttcacaggc
    15181 cggaaaatct cccttgactt ccaagatgtc gaaatccgca ccatcctgca gattttggca
    15241 aaagaatccg ggatgaacat tgttgccagc gactccgtca acggcaaaat gaccctctcc
    15301 ctcaaagacg taccttggga tcaggctttg gatttggtta tgcaggcacg caacctcgat
    15361 atgcgccagc aagggaacat cgtcaacatc gcgccccgcg acgagctgct tgccaaagac
    15421 aaagccttct tacaggcgga aaaagacatt gccgatctgg gcgcgctgta ttcacaaaac
    15481 ttccaattga aatacaaaaa tgtggaagaa ttccgcagca tcctgcgttt ggacaatgcc
    15541 gacacgaccg gaaaccgcaa tacgcttgtc agcggcaggg gcagcgtgct gatcgatccc
    15601 gccaccaata ccctgattgt taccgatacc cgcagcgtca tcgaaaaatt ccgcaaactg
    15661 attgacgaat tggacgtacc cgcgcaacaa gtgatgattg aggcgcgtat cgtcgaagcg
    15721 gcagacggct tctcgcgcga tttgggcgtt aaattcggcg cgacaggcaa gaaaaagctg
    15781 aaaaatgata caagcgcatt cggctggggg gtaaactccg gcttcggcgg cgacgataaa
    15841 tgggaggccg aaaccaaaat caacctgccg attaccgctg ccgcaaacag catttcgctg
    15901 gtgcgcgcga tttcctccgg tgccttgaat ttggaattgt ccgcatccga atcgctttca
    15961 aaaaccaaaa cgcttgccaa tccgcgcgtg ctgacccaaa accgcaaaga ggccaaaatc
    16021 gaatctggtt acgaaattcc tttcaccgta acctcaatcg cgaacggcgg cagcagcacg
    16081 aacacggaac tcaaaaaagc cgtcttgggg ctgaccgtta cgccgaacat cacgcccgac
    16141 ggccaaatca ttatgaccgt caaaatcaac aaggactcgc ctgcgcaatg tgcctccggt
    16201 aatcagacga ttctgtgtat ttcgaccaaa aacctgaata cgcaggctat ggttgaaaac
    16261 ggcggcacat tgattgtcgg cggtatttat gaagaagaca acggtaatac gctgaccaaa
    16321 gtccccctgt tgggcgacat ccccgttatc ggcaacctct ttaaaacacg cgggaaaaaa
    16381 accgaccgcc gcgaactgct gattttcatt accccgagga ttatgggtac ggccggcaac
    16441 agcctgcgct attgatgcgt caaaataagg gcatatgttt tacggcatat gccctttctt
    16501 tatgcttttt gccgcgaccg aaatgccgtc attcccgcgc aggcgggaat ccagtccgtt
    16561 cagtttcggt catttctgat aaattcctgt tgcttttcat ttctggattc ccacttttgt
    16621 gggaatgacg gcggaagggg taaatcctcg caatccaaag cctcgtcatt tccacaaaaa
    16681 acagcaaccc ggaacagcaa cttaaaaccc cgtcattccc gcgca
//
LOCUS       DAWHSK010000016        16144 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.016, whole genome shotgun sequence.
ACCESSION   DAWHSK010000016 DAWHSK010000000
VERSION     DAWHSK010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16144
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.016"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(86..2191)
                     /gene="fusA"
                     /locus_tag="WIC03_000394"
     CDS             complement(86..2191)
                     /gene="fusA"
                     /locus_tag="WIC03_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEZ3882950.1"
                     /translation="MARKTPISLYRNIGISAHIDAGKTTTTERILFYTGLTHKLGEVH
                     DGAATTDYMEQEQERGITITSAAVTSYWSGMAKQFPEHRFNIIDTPGHVDFTVEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYQVPRLAFVNKMDRQGANFFRVVEQMKT
                     RLRANPVPIVIPVGAEDNFSGVVDLLKMKSIIWNEADKGTTFTYGDIPAELVETAEEW
                     RQNMIEAAAEASEELMDKYLGGDELTEEEIVGALRQRTLAGEIQPMLCGSAFKNKGVQ
                     RMLDAVVELLPAPTDIPPVQGVNPNTEEADSRQASDEEKFSALAFKMLNDKYVGQLTF
                     IRVYSGVVKSGDTVLNSVKGTRERIGRLVQMTAADRTEIEEVRAGDIAAAIGLKDVTT
                     GETLCAESAPIILERMEFPEPVIHIAVEPKTKADQEKMGIALNRLAKEDPSFRVRTDE
                     ESGQTIISGMGELHLEIIVDRMKREFGVEANIGAPQVAYRETIRKAVKAEYKHAKQSG
                     GKGQYGHVVIEMEPMEPGGEGYEFIDEIKGGVIPREFIPSVDKGIRDTLPNGIVAGYP
                     VVDVRIRLVFGSYHDVDSSQLAFELAASQAFKEGMRQASPALLEPIMAVEVETPEEYM
                     GDVMGDLNRRRGVVLGMDDDGIGGKKVRAEVPLAEMFGYSTDLRSATQGRATYSMEFK
                     KYSEAPAHIAAAVTEARKG"
     gene            complement(2210..2680)
                     /gene="rpsG"
                     /locus_tag="WIC03_000395"
     CDS             complement(2210..2680)
                     /gene="rpsG"
                     /locus_tag="WIC03_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEZ3882951.1"
                     /translation="MPRRREVPKRDVLPDPKFGSVELTKFMNVLMIDGKKSVAERIVY
                     GALEQIEKKTGKVAIEVFNEAIANAKPIVEVKSRRVGGANYQVPVEVRPSRRLALAMR
                     WVRDAARKRGEKSMDLRLAGELIDASEGRGGALKKREEVHRMAEANKAFSHFRF"
     gene            complement(2798..3169)
                     /gene="rpsL"
                     /locus_tag="WIC03_000396"
     CDS             complement(2798..3169)
                     /gene="rpsL"
                     /locus_tag="WIC03_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEZ3882952.1"
                     /translation="MPTINQLVRKGRQKPVYVNKVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCKVRLTNGFEVISYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGSL
                     DTAGVKDRKQARSKYGAKRPK"
     gene            complement(3348..3497)
                     /locus_tag="WIC03_000397"
     CDS             complement(3348..3497)
                     /locus_tag="WIC03_000397"
                     /inference="COORDINATES: protein motif:HMM:NF016462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ3882953.1"
                     /translation="MSAALWHKKLSWKGFTPQINFRYNKINSNMPAFYSRSGKERFVS
                     IEKTY"
     gene            complement(3494..3751)
                     /locus_tag="WIC03_000398"
     CDS             complement(3494..3751)
                     /locus_tag="WIC03_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3882954.1"
                     /translation="MKPRNLFFAGCLLTSATFAEDIGVPVELINVGNRIAMPSEGESL
                     ALLPFAEDVPPVRDAMPSEVPKSAAGGDVRGDRIGMPINIG"
     gene            complement(4043..8218)
                     /gene="rpoC"
                     /locus_tag="WIC03_000399"
     CDS             complement(4043..8218)
                     /gene="rpoC"
                     /locus_tag="WIC03_000399"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEZ3882955.1"
                     /translation="MNLLNLFNPLQTAGMEEEFDAIKIGIASPETIRSWSYGEVKKPE
                     TINYRTFKPERDGLFCAKIFGPVKDYECLCGKYKRLKFKGVTCEKCGVEVTLSKVRRE
                     RMGHIELAAPVAHIWFLKSLPSRLGMVLDMTLRDIERVLYFEAFVVTDPGMTPLQRRQ
                     LLTEDDYYNKLDEYGDDFDAKMGAEGIRELLRTLNVAGEIEILRQELESTGSDTKIKK
                     IAKRLKVLEAFHRSGMKLEWMIMDVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVIN
                     RNNRLKRLLELHAPDIIVRNEKRMLQEAVDSLLDNGRRGKAMTGANKRPLKSLADMIK
                     GKGGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIFHKLEKQGL
                     ASTVKAAKKLVEQEVPEVWDILEEVIREHPIMLNRAPTLHRLGIQAFEPILIEGKAIQ
                     LHPLVCAAFNADFDGDQMAVHVPLSLEAQMEARTLMLASNNVLSPANGEPIIVPSQDI
                     VLGLYYMTRDRINAKGEGSLFADVKEVHRAYHTKQVELGTKITVRLREWVKNEAGEFE
                     PVVNRYETTVGRALLSEILPKGLPFEYVNKALKKKEISKLINASFRLCGLRDTVIFAD
                     HLMYTGFGFAAKGGISIAVDDMEIPKEKAALLAEANAEVKEIEDQYRQGLVTNGERYN
                     KVVDIWGRAGDKIAKAMMDNLSKQKVIDRAGNEVDQESFNSIYMMADSGARGSAAQIK
                     QLSGMRGLMAKPDGSIIETPITSNFREGLTVLQYFIATHGARKGLADTALKTANSGYL
                     TRRLVDVTQDLVVVEDDCGTSDGFVMKAVVQGGDVIEALRDRILGRVTASDVVDPSSG
                     ETLVEAGTLLTEKLVDMIDQSGVDEVKVRTPITCKTRHGLCAHCYGRDLARGKLVNAG
                     EAVGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAASQVEAKSNGTARFSSQMRYVANN
                     KGELVVIGRSCEVVIHDDIGRERERHKVPYGAILLVQDGMAIKAGQTLATWDPHTRPM
                     ITEHAGMVKFENVEEGVTVAKQTDDVTGLSTLVVIDGKRRSSSASKLLRPTVKLLDEN
                     GVEICIPGTSTPVSMAFPVGAVITVREGQEIGKGDVLARIPQASSKTRDITGGLPRVA
                     ELFEARVPKDAGMLAEITGTVSFGKETKGKQRLIVTDVDGVAYETLISKEKQILVHDG
                     QVVNRGETIVDGAVDPHDILRLQGIEALARYIVQEVQEVYRLQGVKISDKHIEVIIRQ
                     MLRRVNIADAGETGFITGEQVERGDVMAANEKALEEGKEPARYENVLLGITKASLSTD
                     SFISAASFQETTRVLTEAAIMGKQDELRGLKENVIVGRLIPAGTGLTYHRSRHQQWQE
                     VEQETAETQVTDE"
     gene            complement(8375..12553)
                     /gene="rpoB"
                     /locus_tag="WIC03_000400"
     CDS             complement(8375..12553)
                     /gene="rpoB"
                     /locus_tag="WIC03_000400"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEZ3882956.1"
                     /translation="MNYSFTEKKRIRKSFAKRENVLEVPFLLATQIDSYAKFLQLENA
                     FDKRTDDGLQAAFNSIFPIVSHNGYARLEFVHYTLGEPLFDIPECQLRGITYAAPLRA
                     RIRLVILDKEASKPTVKEVRENEVYMGEIPLMTPSGSFVINGTERVIVSQLHRSPGVF
                     FEHDKGKTHSSGKLLFSARIIPYRGSWLDFEFDPKDLLYFRIDRRRKMPVTILLKALG
                     YGNEQILDIFYDKETFYLSSNGVQTDLVAGRLKGETAKVDILDKEGNVLVAKGKRITA
                     KNIRDITNAGLTRLDVEPESLLGKALAADLIDSETGEVLASANDEITEELLAKFDING
                     VKEITTLYINELDQGAYISNTLRTDETAGRQAARVAIYRMMRPGEPPTEEAVEQLFNR
                     LFFSEDSYDLSRVGRMKFNTRTYEQKLSEAQQNSWYGRLLNETFAGAAEKGGYVLSVE
                     DIVASIATLVELRNGHGEVDDIDHLGNRRVRSVGELTENQFRSGLARVERAVKERLNQ
                     AESENLMPHDLINAKPVSAAIKEFFGSSQLSQFMDQTNPLSEVTHKRRVSALGPGGLT
                     RERAGFEVRDVHPTHYGRVCPIETPEGPNIGLINSLSVYARTNDYGFLETPYRRVIDG
                     KVTKEIDYLSAIEEGRYVIAQANADLDSDGNLIGDLVTCREKGETIMATPDRVQYMDV
                     ATGQVVSVAASLIPFLEHDDANRALMGANMQRQAVPCLRPEKPMVGTGIERSVAVDSA
                     TAIVARRGGVVEYVDANRVVIRVHDDEATAGEVGVDIYNLVKFTRSNQSTNINQRPAV
                     KAGDVLQRGDLVADGASTDLGELALGQNMTIAFMPWNGYNYEDSILISEKVAADDRYT
                     SIHIEELNVVARDTKLGAEDITRDIPNLSERMQNRLDESGIVYIGAEIEAGDVLVGKV
                     TPKGETQLTPEEKLLRAIFGEKASDVKDTSLRMPTGMSGTVIDVQVFTREGIQRDKRA
                     QSIIDSELKRYRLDLNDQLRIFDNDAFDRIERMIVGQKANGGPMKLAKGSEITTEYLA
                     GLPSRHDWFDIRLTDEDLAKQLELIKVSLQQKREEADELYEIKKKKLTQGDELQPGVQ
                     KMVKVFIAIKRRLQAGDKMAGRHGNKGVVSRILPVEDMPYMADGRPVDIVLNPLGVPS
                     RMNIGQILEVHLGWAAKGIGERIDRMLKEQRKAGELREFLNRLYNGSGKKEDLDALTD
                     EEIIELASNLRKGASFASPVFDGAKESEIREMLNLAYPSDDPEVEKLGFNDSKTQITL
                     YDGRSGEAFDRKVTVGVMHYLKLHHLVDEKMHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYENIVKGEHKIDAGMPESFNVLVK
                     EIRSLGLDIDLERY"
     gene            complement(12743..13114)
                     /gene="rplL"
                     /locus_tag="WIC03_000401"
     CDS             complement(12743..13114)
                     /gene="rplL"
                     /locus_tag="WIC03_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEZ3882957.1"
                     /translation="MAITKEDILEAVGSLTVMELNDLVKAFEEKFGVSAAAVAVAGPA
                     GAGAADAEEKTEFDVVLASAGDQKVGVIKVVRAITGLGLKEAKDIVDGAPKTIKEGVS
                     KAEAEDIQKQLEEAGAKVEIK"
     gene            complement(13172..13672)
                     /gene="rplJ"
                     /locus_tag="WIC03_000402"
     CDS             complement(13172..13672)
                     /gene="rplJ"
                     /locus_tag="WIC03_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEZ3882958.1"
                     /translation="MSLNIETKKVAVEEISAAIANAQTLVVAEYRGISVSSMTELRAN
                     ARKEGVYLRVLKNTLARRAVQGTSFAELADQMVGPLVYAASEDAVAAAKVLHQFAKKD
                     DKIVVKAGSYNGEVMNAAQVAELASIPSREELLSKLLFVMQAPVSGFARGLAALAEKK
                     AGEEAA"
     gene            complement(13902..14597)
                     /gene="rplA"
                     /locus_tag="WIC03_000403"
     CDS             complement(13902..14597)
                     /gene="rplA"
                     /locus_tag="WIC03_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEZ3882959.1"
                     /translation="MAKVSKRLKALRSSVEANKLYAIDEAIALVKKAATAKFDESVDV
                     SFNLGVDPRKSDQVIRGSVVLPKGTGKITRVAVFTQGANAEAAKEAGADIVGFEDLAA
                     EIKAGNLNFDVVIASPDAMRIVGQLGTILGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VQYRTDKAGIVHATIGRASFAEADLKENFDALLDAIVKAKPAAAKGQYLKKVAVSSTM
                     GLGIRVDTSSVNN"
     gene            complement(14597..15031)
                     /gene="rplK"
                     /locus_tag="WIC03_000404"
     CDS             complement(14597..15031)
                     /gene="rplK"
                     /locus_tag="WIC03_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEZ3882960.1"
                     /translation="MAKKIIGYIKLQIPAGKANPSPPVGPALGQRGLNIMEFCKAFNA
                     ATQGMEPGLPIPVVITAFADKSFTFVMKTPPASILLKKAAGLQKGSSNPLTNKVGKLT
                     RAQLEEIAKTKDPDLTAADLDAAVRTIAGSARSMGLDVEGVV"
     gene            complement(15132..15668)
                     /gene="nusG"
                     /locus_tag="WIC03_000405"
     CDS             complement(15132..15668)
                     /gene="nusG"
                     /locus_tag="WIC03_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEZ3882961.1"
                     /translation="MSKKWYVVQAYSGFEKNVQRILEERIAREEMGDYFGQILVPVEK
                     VVDIRNGRKTISERKSYPGYVLVEMEMTDDSWHLVKSTPRVSGFIGGRANRPTPISQR
                     EAEIILQQVQTGIEKPKPKVEFEVGQQVRVNEGPFADFNGVVEEVNYERNKLRVSVQI
                     FGRETPVELEFSQVEKIN"
     gene            complement(15670..15948)
                     /gene="secE"
                     /locus_tag="WIC03_000406"
     CDS             complement(15670..15948)
                     /gene="secE"
                     /locus_tag="WIC03_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEZ3882962.1"
                     /translation="MTEHTPEKKNVKVDQLVVQDKESASNSGKEGFFAYFSNSWSEFK
                     KVVWPKREDAVRMTVFVIVFVAVLSIFIYAADTAISWLFFDVLLRREG"
     gene            complement(16064..16139)
                     /locus_tag="WIC03_000407"
     tRNA            complement(16064..16139)
                     /locus_tag="WIC03_000407"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16104..16106),aa:Trp,seq:cca)
BASE COUNT         4079 a         4182 c         3577 g         4306 t
ORIGIN      
        1 gctaattcct ttacattaaa gatcgattaa agaacaatgc cgtctgaaaa atcatttcag
       61 cctttcggac ggcctatttg cctgattagc ctttacgggc ttcagttaca gcagcagcta
      121 tgtgagcagg agcttcagaa tatttcttga actccataga gtaagtagcg cggccttggg
      181 ttgcagaacg caggtcggtc gaataaccga acatttctgc cagaggtact tcggcacgga
      241 cttttttacc gccgataccg tcatcatcca tacccaatac aacaccgcga cggcggttca
      301 agtcgcccat tacgtcgccc atgtattctt ccggagtttc cacttcaact gccataatcg
      361 gctcaagcag ggcaggagat gcttgacgca taccttcttt aaacgcttga gaagcggcca
      421 attcaaatgc caattgggaa gagtcgacat catggtaaga accgaatacc agacggatac
      481 gtacatcaac tacaggatag ccggcaacga taccgttagg caatgtatcg cggatacctt
      541 tatcgacaga cggaatgaat tcgcgaggaa tcacgccacc tttaatttca tcgataaact
      601 cgtaaccttc gccccccggt tccataggtt ccatttcaat cacaacgtga ccatattgac
      661 ctttaccacc ggattgtttc gcgtgtttgt attcagcttt aacggctttg cggatagttt
      721 cacggtaagc cacttgaggt gcaccgatat ttgcttccac accgaattcg cgtttcatac
      781 ggtcaacaat aatttccaag tgcagctcac ccataccgga aataatggtt tgaccggatt
      841 cttcgtctgt acggacacgg aaagaagggt cttctttagc caagcggttc agggcgatac
      901 ccattttctc ttggtcggct ttggttttcg gctcaacggc aatatggatt accggctcgg
      961 ggaattccat acgttccaag ataatcggcg cgctttccgc acacaaggtt tcaccggtag
     1021 taacgtcttt cagaccaata gcggctgcga tgtcgccggc gcgtacttct tcgatttcag
     1081 tacggtctgc ggcagtcatt tgtaccaaac gaccgatacg ttcgcgagtg ccttttacgg
     1141 agttcaatac ggtatcgccg gattttacta cgcctgagta aacgcggata aaggtcagct
     1201 gaccgacgta tttgtcgttc aacattttga acgccaatgc agagaatttc tcttcatcgc
     1261 tggcttgacg gctgtcggct tcctcggtat tcgggttgac accttgaacc ggaggaatat
     1321 cggtaggagc tggcagcaat tctacaactg cgtccaacat acgttgaaca cctttgtttt
     1381 taaatgcaga accacacagc ataggctgaa tttcgcctgc caaagtacgt tgacgcaacg
     1441 cgcctacgat ttcttcttcg gtcagctcgt cgccgcctaa gtatttgtcc atcagttctt
     1501 cgctggcttc ggctgcggct tcaatcatat tttgacgcca ttcttcggca gtttcgacca
     1561 attcggcagg aatatcgcca taggtaaagg ttgtaccttt atcggcttca ttccaaatga
     1621 tggatttcat tttcaacaaa tcaaccacac cgctgaagtt gtcttccgca ccaaccggaa
     1681 tgacgatagg tacagggttt gcgcgcaaac gggttttcat ttgctcgaca acgcggaaga
     1741 agttggcacc ctgacggtcc attttattga caaacgccaa gcgcggcact tggtatttgt
     1801 tggcttgccg ccatacggtt tcagattggg gttgaacacc gcccaccgcg cagtaaacca
     1861 ttaccgcgcc gtccaataca cgcatagaac gctctacctc tacggtaaag tcaacgtgtc
     1921 ccggggtgtc gatgatgttg aagcggtgct cggggaattg tttcgccata ccggaccagt
     1981 aggaagtaac ggcagcggag gtaatggtaa taccgcgctc ttgctcttgt tccatgtagt
     2041 cggtagtagc cgcaccgtca tgcacttcgc ccagcttgtg ggtcaaaccg gtatagaaca
     2101 aaatacgttc tgtcgtcgtg gttttacccg catcgatatg ggcggaaata ccgatgttac
     2161 ggtacaggct gatcggggtc ttacgagcca ttttattagc ctttcaaaat tagaaacgga
     2221 agtgagagaa tgctttgttg gcttcagcca tacggtgtac ttcttcacgt tttttcaacg
     2281 caccgccacg gccttcggac gcatcaatca attcgcctgc caaacgcagg tccatggatt
     2341 tctcaccacg tttgcgggcc gcatcgcgaa cccagcgcat tgccaaagcc aaacggcgtg
     2401 aaggacgaac ttcaacagga acttggtagt ttgcaccacc tacacggcgg cttttcactt
     2461 ccacgatagg tttggcgttt gcaatggctt cgttaaatac ttcgattgct actttgccgg
     2521 tttttttctc aatctgttcc aacgcaccgt aaacgatacg ctcggcaacg gattttttac
     2581 cgtcaatcat caatacgttc atgaatttgg tcaactcgac gctgccgaat ttaggatctg
     2641 gcagtacgtc gcgcttgggg acttctctac gtcttggcat tttaatttcc tttaatctat
     2701 tcagtcgggt ctattcccat gaatacccaa ttgagtattc acttactcgg ccgttgttca
     2761 gcttaggcgg ccgacgtgcc tatttaagtc ccagtaatta tttaggacgc ttagcaccgt
     2821 atttggaacg ggcttgttta cggtctttaa cgcctgcagt atccaaagaa ccgcgtacag
     2881 tgtggtaacg cacacctggc aagtctttta cacgaccgcc gcgaatcaat acgacactgt
     2941 gctcttgcag gttgtgacct tcgccgccga tgtatgaaat gacttcaaaa ccgttggtca
     3001 ggcggacttt acatacttta cgcaatgcag agttaggttt tttaggggta gtcgtgtata
     3061 cacgggtgca cacgccgcgt ttttgcgggc aggcttccag tgcgggcact ttgtttacgt
     3121 acacgggctt ttgacggcct ttgcgtacca attggttgat agttggcata ttttctcgtc
     3181 ctgttgagtt aaatacttgc cgacaccatg tcggcaagag tggaattata tttttattgc
     3241 aagtatacag tcaagcataa cgacaagaag aaagatgatt ttcttgtttg ccgataaata
     3301 tttaccgaca tccctcacaa tcagtcggac gactgaagaa aatactgtca atacgttttt
     3361 tctatactga caaaccgttc cttgccgctg cgcgaataaa aggcgggcat attgctgttg
     3421 atcttgttgt aacggaaatt tatttggggt gtaaatcctt tccacgacag ttttttatgc
     3481 cataaagccg cgctcatccg atgttaatcg gcattcctat ccggtcaccc cgaacatcgc
     3541 cgcctgccgc gcttttagga acttcagacg gcattgcatc gcgaaccggc ggtacatcct
     3601 cggcaaacgg caggagggcg aggctttccc cttcagacgg catcgcaatc cgattaccga
     3661 cgttaatcag ttcgacaggt acgccgatat cctcggcaaa cgtcgccgaa gtcagcaggc
     3721 agcctgcaaa aaagagattt ctgggtttca tatttatttt ttaaaggaaa tgccgtctga
     3781 agtttcagac gacatttgtt taaatggaaa acattaagac gctacttttt ttgaccgccg
     3841 aaaccaacat ccctagcaaa gttattttgg tctccggacg taccgacgac acccgccacc
     3901 tcgttggtct ccggcccgaa aatacctaag gaataatcca cacgcctcaa aatgacgaaa
     3961 acgcttgttt ttgccttaat cagacaaaga aaaaccgcaa gcttaaagct tgcggttttg
     4021 tattgaataa acgcaccaaa gattattcat ccgttacttg ggtttcagca gtctcctgtt
     4081 ccacctcttg ccattgttga tgacggctgc ggtggtaagt caaaccggta ccggcaggaa
     4141 tcaggcggcc gacgatgacg ttctctttta aaccgcgcag ttcgtcttgt ttgcccataa
     4201 tcgcggcttc ggtcagaaca cgggtcgttt cttggaacga tgcggcagaa atgaaactgt
     4261 cggtggacag ggaggctttg gtaataccca gcaatacgtt ttcgtaacgt gccggctctt
     4321 tgccttcttc caaagctttt tcattggccg ccatcacatc gccgcgttcg acctgctctc
     4381 cggtaatgaa cccggtttcg ccggcatccg caatgttcac acggcgcaac atttgacgga
     4441 tgatgacttc gatgtgttta tcagaaatct tcacaccttg cagacggtaa acctcttgca
     4501 cctcttggac aatgtagcgt gccagtgctt cgataccttg caaacgcaga atatcatgcg
     4561 gatcaacagc tccgtccacg atggtttcac cgcggtttac cacttgaccg tcgtgtacca
     4621 gaatttgttt ctctttggaa atcaaggtct cgtatgctac accgtccacg tcagtaacaa
     4681 tcagacgttg cttgcctttg gtctctttgc cgaaggaaac ggtaccggta atttccgcca
     4741 acataccggc atctttcggc acgcgtgctt caaacaattc ggcaacgcgc ggcagaccac
     4801 cggtaatgtc gcgggttttg gaagaggctt gcgggatacg cgccaatacg tcgcctttac
     4861 cgatttcctg accttcgcgt acggtaatca ccgcaccaac ggggaatgcc atcgaaaccg
     4921 gagtggacgt accgggaata cagatttcca cgccgttttc atccaacaat tttacagtcg
     4981 gacgcagcag tttggacgcg ctgctggaac ggcgtttacc gtcaatcacc accaaagtgg
     5041 acaaaccggt tacatcgtca gtttgtttcg caacggtaac accctcttcc acgttctcaa
     5101 atttcaccat acctgcatgt tcggtgatca tcggacgggt atgcggatcc caggttgcca
     5161 aggtttgacc ggctttaatg gccataccgt cttgtaccag caggatggca ccgtaaggta
     5221 ctttgtggcg ttcgcgttca cggccgatat cgtcgtgaat cacgacttca caagaacggc
     5281 cgatgacaac caactcgcct ttattgttgg caacataacg catttggctg ctgaatcgtg
     5341 ccgtaccgtt ggatttggct tccacttggc tggctgctgc cgcacgggat gccgcaccac
     5401 cgatgtggaa cgtacgcata gtcagctggg tacccggttc gccgattgat tgggcggcaa
     5461 tcacaccaac tgcctcaccg gcattgacca gcttaccacg tgccaagtcg cgaccataac
     5521 agtgcgcgca caaaccgtgg cgggttttac aagtaatcgg cgtacggact ttgacttcat
     5581 cgacaccgga ttggtcaatc atatccacca gtttttcagt caacaacgta ccggcttcaa
     5641 ccaaggtctc gccgcttgac ggatcgacaa cgtcagacgc agtaacacgg cccaaaatac
     5701 gatcgcgcaa tgcttcaatc acatcaccgc cttgtaccac tgccttcata acaaagccgt
     5761 ctgaagtacc gcaatcgtct tcaacaacga ccaaatcttg agttacgtct accagacgac
     5821 gggtcaggta accggagttc gcggttttca atgcggtatc cgccaaaccc ttacgcgcac
     5881 cgtgggtcgc aataaagtat tgcaatacgg tcagaccttc gcggaagttt gaggtaatcg
     5941 gcgtttcaat aatcgagccg tcaggtttcg ccatcaaacc gcgcatacct gacaactgtt
     6001 taatctgagc cgcagaacca cgggcaccgg agtctgccat catatagatg gagttgaatg
     6061 actcttgatc gacttcgttg ccggcacggt cgataacttt ttgtttggac aggttgtcca
     6121 tcatcgcctt agcgatttta tcgccggcac gaccccaaat atcgaccacc ttgttgtagc
     6181 gttcgccgtt ggtaaccaaa ccttgacggt attggtcttc gatttcttta acctcggcat
     6241 tggcttcagc cagcaaggcc gctttttctt ttggaatttc catatcgtca acggcaatgg
     6301 aaataccgcc ttttgccgca aatccgaaac cggtgtacat caggtggtca gcaaagataa
     6361 ccgtatcgcg caagccgcac aggcggaacg atgcgttaat cagtttagaa atttcttttt
     6421 tcttcaacgc tttgttgaca tattcaaacg gcaggccttt cggcaggatt tcgctcaaca
     6481 atgcacggcc gacggttgtt tcgtaacggt taacgacagg ctcaaactca cctgcttcgt
     6541 ttttcaccca ttcgcgcaga cgtacggtga tttttgtacc cagctcgacc tgtttggtat
     6601 ggtatgcgcg atgcacttct ttcacatcgg caaacaggct gccttcgcct ttggcattga
     6661 tacggtcgcg ggtcatgtag tacagaccca atacgatatc ttgggaaggt acaataatcg
     6721 gttcgccgtt ggcaggagac aatacgttgt ttgaagccag catcagcgtg cgtgcttcca
     6781 tttgtgcttc caagctcaat ggaacgtgta ccgccatttg gtcgccgtca aagtcggcgt
     6841 tgaacgcggc acacaccaat gggtgcaact gaatcgcttt accttcaatc aagataggtt
     6901 caaacgcttg aatacccaaa cggtgcaggg tcggcgcacg gttcagcata atcggatgtt
     6961 cgcggatgac ttcttccaag atgtcccata cttccggtac ttcttgctct accaattttt
     7021 tcgctgcttt aacggtggaa gccagacctt gtttttccaa tttgtggaaa atgaacggtt
     7081 tgaacagttc caaagccatt tttttcggca gaccgcattg gtgcagacgc aggtatgggc
     7141 ctacggtaat cacggaacgg ccggagtagt ccacacgttt gcccaacagg ttttgacgga
     7201 agcgaccgcc tttaccttta atcatgtctg ccaatgattt cagcgggcgt ttgttggcgc
     7261 cggtcatggc tttaccgcga cggccgttat ccaacagcga gtcaactgct tcttgcaaca
     7321 tacgtttttc gttgcggacg atgatgtcgg gcgcatgcag ttccaacaga cgtttcagac
     7381 ggttgttacg gttaataacg cggcggtaca aatcgttcaa atcggaagtg gcaaaacgac
     7441 caccatccaa tggaaccaac ggacgcaaat caggcggcaa taccggcagc acatccataa
     7501 tcatccattc cagtttcata ccggaacgat ggaaggcttc caatactttc aagcgtttgg
     7561 cgattttttt gattttggtg tcggaaccgg tcgattccaa ctcttggcgc aggatttcga
     7621 tttcgcccgc tacattcagg gtacgcagca attcgcggat accttccgca cccattttgg
     7681 catcgaaatc gtcgccgtat tcgtccagct tgttgtagta atcgtcttca gtcagcaatt
     7741 ggcggcgttg cagcggagtc ataccgggat cggttaccac aaatgcttca aagtacaata
     7801 cgcgctcgat gtcgcgcaaa gtcatgtcta acaccatacc caagcgggaa ggcagggatt
     7861 ttaagaacca aatatgtgcg acgggcgcag ccaattcgat atgacccatg cgttcgcggc
     7921 gcactttgga cagggttact tccacgccgc atttttcaca cgttacgcct ttaaatttca
     7981 agcgtttgta ttttccgcac aagcattcgt agtctttgac cgggccaaag attttggcac
     8041 agaacaaacc gtcacgctca ggtttgaacg tacggtagtt gatggtttca ggttttttga
     8101 cttcgccata agaccatgag cggatggttt cgggagaggc aataccgatt ttaatggcat
     8161 caaactcttc ttccatgccg gcagtttgca acggattaaa taagttcaac aaattcattt
     8221 ttgctccttg aaggaagtat ttttaccgca tggcggattt tcgatattgg gaaacggtac
     8281 ttttgatttc agacggccta aatgccgatt gcttcattct tattctgaaa ccactttttc
     8341 agacgaccct gaaaggccgt ctgaaaactt ttgtttagta acgttccaaa tcgatatcca
     8401 agcccagtga gcgaatctct ttgaccaata cgttgaagga ctcgggcata ccggcatcga
     8461 ttttgtgttc gcctttgacg atgttttcgt acattttggt acggccgttc acgtcgtcag
     8521 acttcacagt cagcatctct tgcagcgtgt atgccgcgcc gtatgcttcc aatgcccaaa
     8581 cctccatctc gccgaaacgt tggccgccga actgggcttt accgcccaaa ggctgctggg
     8641 taaccagact gtacggaccg gtagaacgcg cgtgcatttt ttcgtcaacc aagtggtgca
     8701 gtttcagata gtgcatcaca cctactgtaa ccttgcggtc aaatgcttcg cctgaacggc
     8761 cgtcatacag cgtgatttgg gttttactgt cgttgaagcc cagtttttca acctcaggat
     8821 cgtcgctcgg ataagccaag ttcagcattt cgcggatttc agactctttc gcaccgtcga
     8881 atacaggaga ggcgaaagat gcacctttgc gcaggttgga ggccagttcg atgatttctt
     8941 catcagtcag ggcatccaaa tcttctttct taccgctgcc gttgtagagt ctgttcaaga
     9001 actcgcgcaa ctcgcctgct ttgcgttgct ctttcagcat acggtcgata cgctcgccga
     9061 taccttttgc tgcccaaccc aagtgaactt ccaaaatctg accgatgttc atacgggaag
     9121 gtacgcccaa tgggttcagt acgatgtcta ccggacggcc gtccgccatg taaggcatgt
     9181 cttccactgg cagaatgcgc gataccacgc ctttgttacc gtggcggccc gccattttgt
     9241 cgccggcttg cagacggcgt ttgatggcga taaaaacttt caccattttt tgtacgccgg
     9301 gttgcaattc gtcgccttgg gtcagttttt tcttcttgat ttcgtataac tcgtccgctt
     9361 cttcgcgttt ttgttgcagg ctcactttaa tcagttccaa ctgcttggcc aaatcttcat
     9421 cggtcagacg gatatcgaac caatcgtgcc tgctcggcag acccgccaaa tattcggtag
     9481 taatttcgct gcctttagcc agcttcatcg gaccaccatt ggctttttga cccacaatca
     9541 tacgttcgat acggtcgaat gcgtcgttgt cgaaaatacg caattggtcg ttcaaatcca
     9601 aacggtagcg tttcaactcg gaatcgataa tggattgggc gcgtttgtcg cgttgaatac
     9661 cttcacgagt gaagacttga acgtcgataa ccgtaccgct cataccggta ggcatacgca
     9721 atgaagtatc ttttacgtca gacgcttttt cgccgaagat ggcgcgcaac agtttttctt
     9781 ccggcgtcag ctgggtttcg cctttaggcg ttactttgcc taccaacaca tcgccggctt
     9841 ctatttctgc accgatgtaa acgataccgg attcgtccaa gcggttttgc atacgctcgg
     9901 acaagttcgg aatatcgcgg gtaatgtctt ccgcacccag cttggtatcg cgggcaacga
     9961 cattcaattc ctcaatgtga atcgaagtgt aacgatcatc cgcagccact ttttctgaaa
    10021 tcagaatcga gtcttcatag ttgtaaccgt tccatggcat gaaggcgatg gtcatgtttt
    10081 gacccaaagc caattcgccc aagtcggtag acgcaccgtc ggccaccaag tcgccgcgtt
    10141 gcaacacgtc gcctgctttt acggccggac gttggttgat gttggtagat tggttggaac
    10201 gggtaaattt aaccaagttg taaatatcga cacccacttc gccggcagtc gcttcgtcgt
    10261 catggacacg aatcacaaca cggttggcat cgacatactc gaccacgccg cctcggcggg
    10321 caacgattgc agtagcagag tcaacggcaa cggaacgctc gataccggta ccgaccatcg
    10381 gtttttcagg acgcaagcaa ggcactgcct gacgttgcat attcgcaccc atcaatgcgc
    10441 ggttcgcgtc atcgtgttcc aagaacggaa tcagggatgc tgcaacggat accacttgac
    10501 cagttgccac gtccatatat tggacgcggt cgggcgttgc cataatggtt tcgccttttt
    10561 cacgacaggt aaccaaatcg ccaatcagat tgccatctga atccaaatcg gcattcgcct
    10621 gtgcaatcac atagcggcct tcttcgatgg cagacaagta atcgatttcc ttggttactt
    10681 tgccgtcgat aacgcggcgg taaggcgttt ccaagaaacc gtaatcattg gtgcgcgcat
    10741 aaacggacaa tgagttgatc aaaccgatgt tcggaccttc aggcgtttca atcggacata
    10801 cgcggccgta gtgggtcgga tgcacgtccc gtacctcgaa gccggcacgt tcgcgggtca
    10861 aaccgcccgg acccaatgcg gatacgcggc gtttatgggt tacttcagac aaggggttgg
    10921 tctgatccat aaactgactc aattggctgg agccgaagaa ttctttaata gcggcagaaa
    10981 caggttttgc attaatcaaa tcgtgcggca tcaagttttc tgattccgcc tgattcaaac
    11041 gttcttttac agcacgttcc acacgggcca aaccgctgcg gaattggttt tcagtcagct
    11101 cgcctaccga acgtactcgg cggttgccca agtgatcgat atcgtcaact tcaccatggc
    11161 cgttacgcaa ctcgaccaaa gtcgcaatcg aggcgacaat atcttcgacg ctcaggacat
    11221 aaccgccttt ttcggcagca ccggcaaacg tttcgttcag caggcggccg taccaagagt
    11281 tttgttgggc ttcggacagt ttttgttcgt atgtgcgcgt attaaatttc atacggccta
    11341 cgcgggacaa atcgtagctg tcttcactga agaacaagcg gttaaacaat tgctcgaccg
    11401 cctcttcggt gggcggttcg cccggacgca tcatacggta aatcgcaaca cgcgccgcct
    11461 gccggccggc agtctcatcc gtacgcaagg tgttggagat ataagcaccc tgatccaact
    11521 cattgatgta aagggtcgta atttctttta cgccgttgat atcaaatttg gccaacaact
    11581 cttctgtaat ttcatcattg gcagaagcca atacctcgcc ggtttccgaa tcaatcagat
    11641 cggcagccaa tgctttaccc agcaggcttt ccggttctac atccaaacgg gtcaggcctg
    11701 cattggtaat atcacggata tttttcgcag taatgcgctt acctttggca accaatacat
    11761 tgccttcttt atccaagata tcgaccttgg cagtttcgcc tttcagacgg cctgcgacca
    11821 aatcggtttg aacaccgttt gaagacaaat agaacgtttc tttgtcgtag aaaatatcca
    11881 agatttgctc attgccgtag cccaaagcct tcaacaaaat cgttaccggc attttacggc
    11941 ggcggtcgat acggaaatac agcaaatctt tcgggtcaaa ttcaaaatcc aaccatgaac
    12001 cgcggtaggg aatgatacgg gcggagaata acaacttgcc ggaagagtgc gtcttacctt
    12061 tgtcatgctc gaagaatacg ccgggcgaac ggtgcaactg ggagacaatc acacgctctg
    12121 tgccgttaat cacaaaagaa ccgctcgggg tcatcaacgg aatttcgccc atatacactt
    12181 cgttttcacg aacttctttt accgtcggtt tagatgcttc cttatccaaa atcaccaaac
    12241 ggatacgcgc gcgcaagggg gctgcataag tgattccgcg caactgacat tcgggaatat
    12301 cgaacaaagg ctcgcccaat gtgtaatgca caaactccaa tcgcgcataa ccgttatggc
    12361 tcacaatcgg gaaaatagaa ttaaatgccg cctgcagacc gtcatcggta cgtttgtcaa
    12421 aagcattttc cagctgcaaa aacttcgcat aagaatcaat ttgggttgct agcaagaaag
    12481 gaacttccaa aacattttcc cgctttgcaa aactcttacg gatacgtttt ttctcggtaa
    12541 acgaatagtt catacacact ccgaaaagta attttcaata ataggccgtc tgaaacaacg
    12601 gtacgcatca atctgatatt tacatttatt tgcaatgact tgataaaaac tatgcaaata
    12661 aatgtaaaca atacttgatg tttattttaa gaagcaaaat aaggctggca gaaaactgcc
    12721 agccttcata agaagcatca aattatttga tttcgacttt agcgcctgct tcttccagtt
    12781 gtttttggat gtcttcggct tcagctttag aaacaccctc tttaatggtt ttaggtgcgc
    12841 cgtcaacgat gtctttagct tctttcagac ccaaaccggt aattgcacgg acaactttaa
    12901 tcacgccgac tttttgatca ccggcagaag ccaaaacgac atcaaattcg gttttttctt
    12961 cagcatcggc agcaccggca ccagcaggac ctgcaactgc aacagcagca gcagaaacac
    13021 cgaatttttc ttcaaaagct ttaaccaagt cgttcaattc cattacggtc aaagaaccaa
    13081 ctgcttccaa aatgtcttct ttagtaatag ccatgctatt ttactccaaa tattgtatta
    13141 aaaaataatt gattaacaga aacaaaatcg attaagcggc ttcttcgccg gcttttttct
    13201 ctgccaaagc agccaaaccg cgcgcaaagc ccgatacagg agcttgcata acgaacaaca
    13261 gtttggacaa cagctcttcg cggctcggaa tagaagccaa ctcagcaacc tgagcagcat
    13321 tcattacttc gccattgtaa gaaccggctt taacgacaat tttgtcatct tttttcgcga
    13381 attggtgcaa cactttagca gcagcaacag catcttcaga agcagcgtaa accaacggac
    13441 caaccatttg atcggccaat tctgcaaatg aagtaccttg cactgcacga cgagccaaag
    13501 tatttttcag aacacgcaaa taaacgcctt ctttacgtgc attcgcacga agctcagtca
    13561 tactggaaac actgataccg cgatattcag cgactacgag ggtttgagcg ttagcaattg
    13621 ccgcgctaat ttcctcgacc gccactttct tggtttcaat attgagactc aaggtctacc
    13681 tcccactgtt tataaacagg ataccaaaac gatatcccac cagcgcggta acctaaaaag
    13741 acatcttaca agcaatcttg caatgtgttt caggactacc gtctgcgtag ggcagttacg
    13801 attaaatctt acgatcccta cggtcttgga catctactta attttaagta gcccaaattc
    13861 aagcagccca gaaaaccaaa ctgcttgaaa attccttaag attagttatt tacgcttgat
    13921 gtatcaacgc gaatacccaa acccatggtg ctagacacag caactttttt cagatactga
    13981 cctttagcgg cagcaggctt ggctttaacg atagcatcca gcaacgcatc aaagttctct
    14041 ttcaaatcag cttcagcgaa tgaagcacga ccaatcgttg catgaacaat acctgccttg
    14101 tcggtacggt actgcacttg acctgctttt gcatttttta ctgcttcggc aacattaggg
    14161 gtaaccgtac ctactttagg gtttggcatc agaccacgag gacccaagat ggtacccaat
    14221 tgaccaacaa tacgcattgc atcgggagaa gcaataacaa cgtcaaagtt cagattgcct
    14281 gctttgattt cagcagccaa atcttcgaaa ccgacgatat ctgcaccagc ttctttagca
    14341 gcttctgcat ttgcaccttg agtaaataca gccacacggg ttatcttacc ggtgccttta
    14401 ggcagaacga ccgaaccacg gataacttgg tcagatttac gcggatcaac gcccaagttg
    14461 aaagatacgt caacagactc gtcaaattta gcagtcgctg ctttttttac caaagcaatt
    14521 gcttcatcaa ttgcatataa tttattggct tccacagaag agcgaagagc tttcaagcgt
    14581 ttagatactt tagccattat acaacaccct ccacatccaa gcccattgag cgagcagaac
    14641 ctgctatagt acggacagcc gcatccaagt cagcagcagt caaatcagga tctttagttt
    14701 tagcaatttc ttccaactgg gcacgggtca atttacccac tttgttggtc agaggattag
    14761 aactaccttt ttgcaaaccg gcagcctttt tcaacaagat agaagctggc ggggttttca
    14821 tcacaaatgt gaatgattta tctgcaaatg cagtaatcac aaccggaatc ggtaagccag
    14881 gctccatacc ttgggttgca gcattaaatg ccttacaaaa ttccataata ttcaaaccgc
    14941 gctgacccaa agcaggacca accggaggag atggattggc tttacctgca ggaatttgca
    15001 gtttaatata gccgataatt ttctttgcca cttaacgaac tcctaaaaac gggtaagacg
    15061 cggaaccatt tccgctcccc aagttaattt ttgattctat cctcttattg cttttttttc
    15121 aagtataaaa atcagttaat cttttcaacc tggctgaact ccagctcaac gggtgtttct
    15181 ctaccaaata tctgaacaga cacgcgtaac ttattccgtt cataattgac ctcctcaacc
    15241 accccgttaa aatccgcaaa cggcccttca tttacacgaa cctgttgacc gacctcaaat
    15301 tcaacttttg gtttcggctt ctctatgccg gtctgaacct gctgtaaaat aatttcagcc
    15361 tctctctgac taatcggcgt aggtctatta gccctccctc caataaaacc ggaaacacgg
    15421 ggggtgcttt ttacaagatg ccaagagtca tctgtcattt ccatctcaac tagcacataa
    15481 ccaggatatg actttctttc actaatagtc ttacgaccat tgcggatatc aacaactttc
    15541 tctacaggca ccagaatttg tccgaaataa tctcccatct cctcacgggc aatgcgctct
    15601 tccaatattc gttggacatt cttctcaaac cccgaatacg cctgtacaac ataccatttt
    15661 ttcgacatct caaccttccc ttctcagcaa tacatcaaaa aataaccacg aaattgctgt
    15721 atctgccgca tagataaata tagaaagcac agcaacaaac actataacaa atacagtcat
    15781 tctgacagca tcttcacgct taggccaaac cacctttttg aattcggacc aagaatttga
    15841 gaaatatgca aaaaaccctt ccttaccgga attagatgca gattctttat cttgaacaac
    15901 cagttgatcc actttaacgt tctttttttc aggcgtatgt tctgtcatat tatttatcca
    15961 tcatagcatc tgtctattct caatccatgt aaatggcaag agagtttact aaataacaaa
    16021 tacaaaaaaa attaaccggc acaaggccgg ttaatttttt atttggcagg ccaagagggt
    16081 ctcgaacccc caaccctcgg ttttggagac cgatactcta ccaattgagc tattggcctc
    16141 taaa
//
LOCUS       DAWHSK010000017        15767 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.017, whole genome shotgun sequence.
ACCESSION   DAWHSK010000017 DAWHSK010000000
VERSION     DAWHSK010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15767
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.017"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            212..1579
                     /locus_tag="WIC03_000408"
     CDS             212..1579
                     /locus_tag="WIC03_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ3882963.1"
                     /translation="MQHLTERMTQIAKLLNSSANNPDIDIPDFLTEIKDYSEFSVTDE
                     NGTYLHWDKFRQIHTEDTRMKWRAVKESRKKIQKPIDFPFEHQFWFCIPDSLQARLHL
                     IDKSCGSSIGTSSLGGFGRSEQNRFLLKSLIMEEAITSAQLEGAATTRKVAKDMLKSQ
                     RKPKTKDEIMIVNNYHLMKKAVELKNTPLSVEMILDLHRIATSNAIENKADPGQFRRD
                     DEIFIADNDGNSLYQPPPHGQVHTLMEAVCTFANNTYDGVENPFIHPVVQAIILHFLI
                     GYIHPFGDGNGRIARALFYWFMLKNSYWLFEYISISRLLKNAPAQYAKSYLYAETDDS
                     DLTYFIYYQCDIIKRAVADLEHYISDKQKHQQEFKAAIAQYTEKIGKLNQRQIGILQK
                     AVEESGKIFTALEIANQYGISLNTARSDLSKLGEYRFLVPFKSGNALEYVAPQDLLER
                     LEKKK"
     gene            1981..2445
                     /locus_tag="WIC03_000409"
     CDS             1981..2445
                     /locus_tag="WIC03_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3882964.1"
                     /translation="MLHIPPTEPDRYVSSIVALNVHSSNGTGDWHSAAALNDDAYPQD
                     FYIYGKDQTRSTLHLLGNVGVIDGTNRFNEMGYYPENIPVWLADHPRACVDYLYTAVL
                     QTGSIGRVILDDWFPSDEDKQSVYDLLNQIEPHLNTQEWENLQLWKRKNPIM"
     gene            2572..3861
                     /locus_tag="WIC03_000410"
     CDS             2572..3861
                     /locus_tag="WIC03_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HEZ3882965.1"
                     /translation="MMKSILTVSGNRMRKPRITYLDVWANDERIGTLEKGAMYRFTYD
                     NPNSSLLGLHYQDRSKVYISNNMPHIFAQYFPEGFLAAHITSKYAFHDAPFEDNEMLR
                     LAILGREALGRIHVRCNDPLFNEWIDGLEMKNPRILTERDLLGINARQVFQQYMAEIF
                     HHGRFVSVSGIQQKMSLDAISRNTKQTASYIAKGFDASEYPCLAANEFLCMQTIKQAG
                     IAVAQTSLSEDSSVLLVRRFDVSEQGYFLGMEDFTSLRQYSAEDKYKGSYAAIAQIIR
                     QISGRPDEDLIHFFNQLAASCILKNGDAHLKNFSVLYHDEYDVRLAPAYDVLDTSIYR
                     VGTQGIFDAYDDTLALNLTNHGKKTYPSKNTLLDFAEKYCDLGREDASFMIDTIVQAK
                     EQVLVKYSDVLRENEWLAQKWHFIPDENEEGLPFTFR"
     gene            complement(4186..5973)
                     /locus_tag="WIC03_000411"
     CDS             complement(4186..5973)
                     /locus_tag="WIC03_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HEZ3882966.1"
                     /translation="MKLPLSYLPNIRFLSWCCLLAGIIAPATLLASPNPAEIRMQQDI
                     QQRQREEQLRQTMQPESDVRLHQKNTGETVNQLMGDDSSQPCFAINEVVLEGEHHARF
                     QFALKRALRETGFQAGKCLHAGNINQIMSLAQNALIGRGYTTTRILAAPQDLNSGKLQ
                     LTLIPSYLRSIRIDRSNDDQTHAGRIAAFQNKFPTRSNDLLNLRDLEQGLENLKRLPT
                     AEADLQIVPVEGEPNQSDVVVQWRQRLLPYRVSVGMDNSGSEATGKYQGNITFSADNP
                     FGLSDMFYVNYGRSIGGTPDEESFDGHRKEGGSNNYAVHYSAPFGKWTWAFNHNGYRY
                     HQAVSGLSEVYDYNGKSYNTDFGFNRLLYRDAKRKTYLGVKLWTRETKSYIDDAELTV
                     QRRKTAGWLAELSHKEYIGRSTADFKLKYKRGTGMKDALRAPEEAFGEGTSRMKIWTA
                     SADVNTPFQIGKQLFAYDTSVHAQWNKTPLTSQDKLAIGGHHTVRGFDGEMSLSAERG
                     WYWRNDLSWQFKPGHQLYLGADVGHVSGQSAKWLLGQTLAGTAIGIRGQIKLGGNLHY
                     DIFTGRALKKPEFFQSRKWASGFQVGYTF"
     gene            complement(6028..6486)
                     /locus_tag="WIC03_000412"
     CDS             complement(6028..6486)
                     /locus_tag="WIC03_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-activating lysine-acyltransferase"
                     /protein_id="HEZ3882967.1"
                     /translation="MPITPPLNIISPKLYPNEQWNESEALGAITWLWYQSPTHRQVPI
                     VEMMTYILPVLKNGQFALFCKGTQPIGYISWAYFDEVAQAHYLESDRHLRDNSDWNCG
                     DNIWLIQWFAPLGHSHQMRSAVRQLFPSTTVRALYHKGSDKGLRILTFKT"
     gene            complement(6651..6959)
                     /locus_tag="WIC03_000413"
     CDS             complement(6651..6959)
                     /locus_tag="WIC03_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3882968.1"
                     /translation="MKKNHFNIALLFVSTMMLTGCFGHCADFNCTRLDPRWKKAVETC
                     TTKNRTFVLHELGNQLGYKSITEVSFSEIGQHISSQCIYHSGKYDIHKDKQYGHLFVQ
                     "
     gene            complement(7035..7352)
                     /locus_tag="WIC03_000414"
     CDS             complement(7035..7352)
                     /locus_tag="WIC03_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3882969.1"
                     /translation="MKKTRSNMVLILFCSTILTGCFGSCGDWNCSGNPRWNEKVNDCY
                     SRNLEVVKQQLGYKKYGGDIVRFHSEVQDSEIGQHISTQCIYYSGKYDIHKDKQYGHL
                     FVK"
     gene            complement(7333..8397)
                     /locus_tag="WIC03_000415"
     CDS             complement(7333..8397)
                     /locus_tag="WIC03_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HEZ3882970.1"
                     /translation="MAWSMFATTQADRAVRSATAPKEMWFHKKIIDEKTGKVSFDTRQ
                     IWSLNDLSKEELASIQDTNGKVITVSNPGIFNNREDSLSNAAKQNRNSTNGSGVIAVM
                     NPPTGKYKSDSNNKIKDSLWLSSSLVSELMYVGYDQLNNKVFQGYLPKTNSEKLNQDI
                     YREVQKMGNGWSVDTSNHSRGGITASVSLKDWVNNKEQNGIAPIRKARFYGTATNVKN
                     DYADVLQKNGYTYTGADGKTYNSGAYSIVHDKDFVGNKWIPFLLGNNETTKGACKGFC
                     YSHSSYFAEVPEQYQRDRNGEYILDKNNNKIETEDWKDYKGKWGVPKMGDDGKYINHA
                     IPKLVYPNKLNGEQYEENPF"
     gene            complement(8466..8882)
                     /locus_tag="WIC03_000416"
     CDS             complement(8466..8882)
                     /locus_tag="WIC03_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3882971.1"
                     /translation="MNKNILYIFSLLITIVIFFIFEKNVIRKISFNYNKKEFLISDIT
                     NFNWDYVKLYIINSDFQKIVFYHKSKIVFEELIELDKEGNVLPQYLFDSDLKNVEYYE
                     CDYKNGKMQLLKKEKSHFFDGYFYYYKPINCRPKLL"
     gene            complement(8886..15416)
                     /locus_tag="WIC03_000417"
     CDS             complement(8886..15416)
                     /locus_tag="WIC03_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HEZ3882972.1"
                     /translation="MFASSGNVAIDANGRLVNSGTMAAANAKDTDNTAEHKVNIRSQG
                     VENSGTAVSQQGTQIHSQSIQNTGTLLSSGEILIHNSGSLKNETSGTIEAARLAIDTD
                     TLNNQGKLSQTGSQKLHIDAQGKMDNRGRMGLQDTAPTASNGSSNQTGNSYNASSHSS
                     TTTPTTATGTGTATVSISNITAPTFADGTIRTHGALDNSGSIIANGQTDVSAQQGLNN
                     AGQIDIHQLNAKGSAFDNHNGTIISDAVHIQAGSLNNQNGNITTRQQLEIETGQLDNA
                     HGKLLSAEIADLAVSGSLNNQNGEIATNQQLIIHDGQQSTAAIDNTNGTIQSGRDVAI
                     QAKSLSNNGTLAADNKLDIALQDDFYVGRKIVAGNELSLSTRGSLKNSHTLQAGKRIR
                     IKANNLDNAAQGNIQSGGTTDIGTQHNLTNRGLIDGQQTKIQAGQVNNIGTGRIYGDN
                     IAIAATRLDNQDENGTGAAIAARENLNLGIGQLNNRENSLIYSGNDMAVGGALDTNGQ
                     ATGKAQSIRNASATIEAAGKMRLGVEKLHNTNEHLKTQLVETGREHIVDYEAFGRHEL
                     LREGTQHELGWSVYNDESDHLRTPDRAAHENWHKYDYEQVTQKTQVTQTAPAKIISGN
                     DLTIDGKEVFNTDSQIIAGGNLLVQTEKDGLHNEQTFGEKKVFSENGKLHSYWREKHK
                     GRDSTGHSEQNYTLPEEITRNISLGSFAYESHRKALSHHAPSQGTELLQSNGISLPYT
                     SNSFTPLPSSSLYIINPVNKGYLVETDPRFANYRQWLGSDYMLDSLKLDPNNLHKRLG
                     DGYYEQRLINEQIAELTGHRRLDGYQNDEEQFKALMDNGATAARSMNLSVGIALSAEQ
                     VAQLTSDIVWLVQKEVKLPDGGTQTVLVPQVYVRVKNGDIDGKGALLSGSNTQINVSG
                     SLKNSGTIAGRNALIINTDTLDNIGGRIHAQKSAVTATQDINNIGGILSAEQTLLLNA
                     GNNINSQSTAASSQNTQGSSTYLDRIAGIYITGTEKGVLAAQAGKDINIIASQISNQS
                     EQGQTQLQAGRNINLDTVQTGKHQATHFDADNHVIRGSTNEVGSSIQTKGDVALLSGN
                     NLNAKAAEVGSANGTLAVSAKNDINISAGINTTHVDDASKHKGRSGGGNKLVITDKAQ
                     SHNETAQSSTFDGKQVVLQAGNDANIKGSNVISDNGTHIQAGNHVHIGTTLTHNQSET
                     YHQTKKSGLMSAGIGFTIGSKTNTQENQSQSNEHTGSTVGSLKGDTTIVAGKHYEQIG
                     STVSSPEGNNTIHAQSIDIQAAHNQLNSNTSQTYEQKGLTVAFSSPVTDLAQQAIAVA
                     QSGKQVGQSKNGRVNAMAAANAGWQAYQTGKSAQNLANGTTNAKQVSISITYGEQQNR
                     QTTQVQANQAQASQIQAGGKTTLIATGAAEQSNINIAGSDIAGKAGTILIADNDITLQ
                     SAEQSNTERSQNKSSGWNAGAAVSFGQGGWSLGVTAGGNVGKGHGNGDSVTHRHSHIG
                     DKGSQTVLQSGGDTALKGAQVIGKGVQVNAQNLHIQSVQDSETYQSKQQNASAQVTVG
                     YGFSAGGDYSQSKIRADHVSVTEQSGIYAGEDGYQIKVGNHTDLKGGIITSTQSAEDK
                     GKNRFQTATLTHSDIKNHSQYKGESFGLGASASISGKTLGQGAQNKPQNKHLTSVADK
                     NSASSSVGYGSDSDSQSSITKSGINTQNIQITDEAAQIRLTGKTVAQTKADIDTNVTT
                     DTAERHSGSLKNTFNKEAVQSELDLQRTVSQDFSKNVQQANTEINQHLDKLKADKEAA
                     ETAAAEALANGDMETAKRKVHEAQDAAAKADNWQQGKVILNMLASGLAAPTQSGAGIA
                     AATASPAVSYAIGQHFKDLAGQNANGKLTASQETAHVLAHAVLGAAVAAAGGNNALAG
                     ALSAGGSEAAAPYISKWLYGKEKGSDLTAEEKETVTAITNVLGTATGAAVGNSATDAA
                     QGSLNAKSAVGNNDTVEQVKFALRHPRIAIAIGSVHKDPGSTLEPNISTIASTFQLNL
                     FPNSEFGGEGGVGNAFRHVLWQATITREFGKDIAVKVGNSHESGEKINYSIRRNLSLD
                     KADEMIDQLNNEIGREIALNTNRLNTKELVGLILETYKNNGFYQAERNSNGNYDVVRK
                     RLSEKDYQNTSNILIHLDNTGAGFKIQQRRKQIRAQISARQWRR"
BASE COUNT         3964 a         3416 c         3377 g         5010 t
ORIGIN      
        1 acaccatatt aaaacacagc cttttttaat atagtagaca caatctttcc ctatttatga
       61 aggtgatcgc ttctttcaga ttcgtatttt aatgcttcct atttttataa aaattgacta
      121 gaatagctca attattaaaa attgcgcgat tttggtattt atcatgaaaa tttccagacc
      181 tccggaattt accctgttgc aacaggaata tatgcagcat ctgactgaaa gaatgacgca
      241 aattgccaag ctgctgaatt cttccgcaaa caatcctgat atagacattc ccgattttct
      301 tactgaaatc aaagattatt cagaattttc cgtgacagat gaaaatggaa cctacctgca
      361 ttgggacaaa ttccgccaga ttcacacgga agatacgcgg atgaaatggc gcgccgttaa
      421 ggaaagccgc aaaaaaatcc aaaaaccaat tgatttcccg tttgaacatc agttttggtt
      481 ctgcattccc gactctttgc aggcacggct tcatttgatt gacaaaagct gcggcagttc
      541 tatcggcacg tctagcttgg gtggcttcgg cagaagcgag caaaacagat tcttgctcaa
      601 gtctctgatt atggaagaag cgattacatc cgcccaactg gaaggtgcgg ctaccacgcg
      661 taaagtggcc aaggatatgc tcaaatcgca gcgtaaaccc aaaacaaaag acgaaatcat
      721 gatagtgaac aactatcact tgatgaaaaa agcggtagaa ttgaaaaata cgccgttaag
      781 tgttgaaatg attttggatt tgcaccgcat tgctaccagc aacgctattg aaaacaaggc
      841 cgaccccggg caattcaggc gggacgacga aatctttatc gccgataacg acggcaacag
      901 cctgtaccaa ccaccgccac acgggcaggt tcatacgctg atggaagcgg tatgcacgtt
      961 tgccaacaac acttatgacg gcgtggaaaa tccgtttatc catccggttg tccaagctat
     1021 catcctgcat ttcctgattg gctacatcca tccgttcgga gatggcaacg ggcggatagc
     1081 gcgggctttg ttctattggt ttatgctcaa aaacagctac tggctgtttg aatacatatc
     1141 aatcagccgt cttctgaaaa acgctccggc ccaatacgcc aaatcctatt tgtatgcgga
     1201 aactgacgat tcagatttaa cctatttcat ctattaccaa tgcgatatta tcaagcgggc
     1261 ggttgccgat ttggagcact acatttccga caaacaaaaa caccaacagg aattcaaagc
     1321 agcgattgcc caatatactg aaaagatagg aaagttgaac caacggcaaa ttggtatcct
     1381 gcaaaaagca gtggaagaaa gcggaaaaat ctttactgca ctagaaattg ccaaccaata
     1441 cggcatctcc ctgaatactg cccgtagcga tttgagcaaa ctgggagaat atagattcct
     1501 agtaccgttc aaatcaggaa atgctttaga gtatgttgct cctcaggatt tattggaaag
     1561 gttagaaaaa aaaaaatagt ttgctagcct aaaatgcagc tttaaccgag tcaaaatcaa
     1621 tgcagtccgc acctttaaaa agaagctgcg gactgcttgc tttttgctct acaaatgatc
     1681 tttgtagctg atttaaccaa gattgtagca attttgcttt ccaagcaagc agggttagaa
     1741 aattcgatac ttttaattat tggctgtgtt ttaatatgat gttgataggc gtgtttggct
     1801 ttaaggtatt caacaggata ggtttattcc taaccggaaa ataattgtct tttcccttgc
     1861 aagtaattga aatcaacaga ttactaaaac acagccttac attattgggg tgactatcct
     1921 gtaaaatatg tcctaaaacg tggaaaccac ttttgctctg ctaaatttta aggaatcttt
     1981 atgttacata tacccccaac ggaacctgat cgatatgtta gcagtattgt agccttaaac
     2041 gtgcatagtt ctaacggtac aggcgattgg cacagtgcag cagcccttaa tgatgatgct
     2101 tacccccaag atttctatat ttatggcaaa gatcaaacac gtagcacgtt acatttgctt
     2161 ggtaacgttg gtgtgattga tggaacaaac cgcttcaatg agatgggcta ttatcctgaa
     2221 aacatcccag tttggctcgc agatcacccc cgtgcttgcg tggattatct ttacacagca
     2281 gtgttacaaa ctggctcaat cggtcgggtg attttagatg attggtttcc aagcgatgaa
     2341 gacaagcaat cagtttatga cttacttaac caaatcgaac cgcacttgaa tactcaagaa
     2401 tgggagaatt tacagctatg gaaacgcaaa aacccaataa tgtaatgcta accgaacgag
     2461 ataaaaggca cgagaagcgg tattactgaa tttcatccgc aatacgccat gtacaattaa
     2521 atcagcagat gtgtctaaac gtaccgctgt tcccgtttga aagaaatttt catgatgaag
     2581 tctatcctca ccgtatccgg aaatcgtatg cgtaaaccca gaatcaccta tttggatgtt
     2641 tgggcaaacg atgaaagaat cggtactttg gaaaaggggg ccatgtatcg gttcacatac
     2701 gacaatccca attcttcgtt gctgggcctg cattatcaag acagaagcaa ggtatatatc
     2761 agcaacaaca tgccgcatat ctttgcacag tattttccgg aaggcttttt ggccgcacac
     2821 atcacaagca aatatgcttt tcatgatgcg ccttttgaag acaatgagat gctgcgcttg
     2881 gcaattctgg gcagagaggc tttgggtcgg atacatgtgc gctgtaatga cccgcttttt
     2941 aatgaatgga ttgacgggtt ggagatgaaa aatccaagaa tattgactga acgggatttg
     3001 ctgggcataa atgcccgaca ggtttttcag caatatatgg cagaaatctt ccatcacggc
     3061 cgtttcgtca gtgtatccgg gatacagcag aagatgtcct tagatgccat cagcagaaat
     3121 accaagcaaa ctgcctcata tattgccaaa ggttttgatg catccgaata tccttgcttg
     3181 gctgccaatg aatttttatg catgcagacc atcaaacaag ccggcattgc cgttgcacag
     3241 accagcctgt cggaagattc atcagtctta ttggtacgtc ggtttgatgt cagtgaacag
     3301 gggtatttct taggcatgga agactttacc agtctgcgtc agtattcagc agaagataag
     3361 tataaaggca gttatgcggc tattgcacag attatccgac agatatccgg cagacccgat
     3421 gaagatttaa tccatttctt taaccaactt gccgccagtt gcatattgaa aaacggcgat
     3481 gcacacctca aaaatttttc agtactctat catgacgaat acgatgtacg tcttgcacct
     3541 gcctatgatg tattggatac atcaatatac agggttggaa cacaaggaat ttttgatgct
     3601 tatgacgata cgctggcatt aaacctgact aaccacggta agaaaacata tccttccaag
     3661 aatacattgt tggattttgc tgagaaatat tgcgatttgg gaagagaaga tgcatccttt
     3721 atgatagata caatcgttca agctaaagaa caggttcttg ttaaatactc ggatgtattg
     3781 cgtgagaatg aatggttggc gcagaagtgg cattttatcc cggatgaaaa tgaagaaggt
     3841 ctaccgttta cattccggta gctgccgctg tcagagatgg ccggtcgact ttcaccctga
     3901 aaatcacttc atcttatggt gtttgaaacc gagaaattag aagaatcgtt ttcggtagga
     3961 gatatactgg gaagattgga aaactggtaa atcactctat tgattgagtt ggcggcctat
     4021 atgtttaatg taggcaaacg agaaggaatc atgtatttca tgattccttc ttaaattcct
     4081 gtgtcaatct aatatcaaaa cacagccatc tcttaccata atcatgatag gtgttttatt
     4141 atgaaaagct atatctatag ttaccgttga tttgactatg ccgttttaaa acgtatagcc
     4201 tacctgaaaa ccgcttgccc atttccttga ttggaaaaat tcgggctttt tcaatgcgcg
     4261 gccggtaaat atatcgtaat gcaggttgcc gccaagcttt atctgcccgc gtatcccaat
     4321 tgctgtgccg gctagagttt ggcccaataa ccatttggcg gattgtcctg aaacatgtcc
     4381 tacatcagcc ccaagataaa gctgatggcc tggtttaaat tgccagctca aatcgttgcg
     4441 ccaataccat ccccgctcgg cagacaaact catttcaccg tcgaagccac gtacggtgtg
     4501 gtgtccgccg atagccagtt tgtcttgcga tgttagcggg gttttgttcc attgtgcatg
     4561 aacggatgtg tcataggcaa atagctgttt accgatttga aaaggagtat ttacatcagc
     4621 cgatgccgtc caaattttca tacgtgacgt gccttctcca aaggcttctt caggcgcgcg
     4681 cagagcatct ttcatgccgg tgccgcgttt atatttcaac ttaaaatctg ccgtactgcg
     4741 gccgatatat tctttgtggg aaagttctgc caaccaaccc gcagttttac gccgttgtac
     4801 agtcagttcg gcatcatcaa tgtaactttt tgtttccctc gtccacagtt ttacaccaag
     4861 ataggttttg cgtttggcat cacgatacaa caggcggttg aagccgaaat cagtattgta
     4921 actttttcca ttatagtcat agacttccga taatccggaa accgcctgat ggtaacggta
     4981 gccattgtga ttgaatgccc atgtccattt accgaaaggg gctgaataat gtacggcgta
     5041 attgtttgat ccgccttctt tgcgatggcc gtcaaaactt tcctcgtcgg gcgtaccgcc
     5101 aattgaacgt ccataattta catagaacat atcactcagt ccaaaaggat tgtcggcaga
     5161 gaaagtgata tttccttggt attttcctgt cgcctcacta cccgaattat ccatccccac
     5221 actcacacgg tagggcagca gacgttgccg ccattgcacc acgacatcac tttggtttgg
     5281 ttctccctct acgggaacga tttggagatc ggcttccgca gtcgggagac gtttgagatt
     5341 ttccagtcct tgttccaaat cacgcagatt caacagatcg ttcgagcggg tgggaaattt
     5401 gttctggaat gctgcaatac gtcctgcatg ggtttgatca tcgttagacc gatcgattcg
     5461 tatggagcgc agatagctcg gtatcagggt taattgaagc ttgccactat tcaaatcctg
     5521 tggcgcagcc aagatacggg tcgtggtata tcccctgccg atcaaagcat tttgtgctaa
     5581 ggacatgatt tgattaatgt tgcccgcatg cagacacttg ccagcctgaa aacccgtttc
     5641 gcgcaaggca cgttttaggg caaactgaaa ccgagcatgg tgttcgcctt ccaacaccac
     5701 ttcgttaatg gcaaaacacg gttggctgct gtcatcgccc atcaactgat taaccgtttc
     5761 ccccgtgttt ttttgatgca aacgcacatc gctttcaggc tgcatggttt ggcgcaactg
     5821 ctcttcgcgt tggcgttgct gaatatcttg ctgcatacgg atttcggcag ggttggggga
     5881 ggccaacaaa gtagcaggag caatgatacc tgccaataag cagcaccaag acaaaaagcg
     5941 aatattaggc aaataggata aaggaagttt catggcatgg tcgcaaaatc aataaaatca
     6001 tcaatgggca ttcaatacac gattgcatca agttttaaaa gttaaaattc tcaaaccctt
     6061 atcgctccct ttatgataca aggcgcgtac tgtcgtacta ggaaataact ggcgcacagc
     6121 tgagcgcatt tgatgactgt gtcccaatgg cgcaaaccat tgaatcagcc aaatattgtc
     6181 gccacagttc caatcgctgt tgtcacgcaa atggcggtca gattctaaat aatgcgcctg
     6241 cgccacttca tcaaaataag cccatgagat ataaccgatt ggttgggtac ccttgcaaaa
     6301 caaagcgaac tgcccgtttt ttaacacagg caatatatac gtcatcatct ccacaatagg
     6361 tacttggcga tgcgtaggcg actgatacca tagccaagtg atggcaccga gtgcttcgct
     6421 ttcgttccat tgttcattgg ggtagagttt aggggagatg atgtttaagg gtggagtgat
     6481 gggcatattt taagttaggg ttcgtgttgg ttaaaacaaa aatggtttca gcctgaaatg
     6541 aaatatttca cgttaaaaca tagctttagc atactagact gaattgtggg gggcaataag
     6601 caacgaccct accattcaac aattctcgtt aattgcacag gttacgctgg ttattgcaca
     6661 aacaaatgac catactgctt atctttatga atatcatact taccgctatg gtaaatacat
     6721 tgagaagaaa tatgttgccc aatttcacta aatgagactt cggtaataga tttatatcct
     6781 aattggttgc ctaattcatg caaaacaaag gtgcgatttt tagttgtgca tgtctcaact
     6841 gctttcttcc agcgtggatc aagtctcgtg caattaaaat ccgcacaatg accaaaacat
     6901 cctgttagca tcatggtgga aacaaaaagt aatgctatat taaaatggtt tttcttcata
     6961 tttttctcca tttggtttgt ttggattgcc taatttagga atagcgtgat ttatgtattt
     7021 gctatcatta ctagctattt cacaaacaaa tgaccatact gcttatcttt atgaatatca
     7081 tatttaccgc tatagtaaat acattgcgta gagatatgtt gtccaatttc gctatcttgc
     7141 acttcgctat ggaaacgaac aatatcacca ccgtattttt tatatcctaa ttgttgctta
     7201 acaacttcaa ggtttctact ataacaatca ttaacttttt cgttccatcg cggattacct
     7261 gaacaattcc agtccccgca actaccaaaa cagccagtta atattgttga gcaaaacagt
     7321 atcaatacca tattagaacg ggttttcttc atattgctca ccattgagtt tattcggata
     7381 cactaatttc ggaatagcat gattgatata tttgccgtca tcacccattt taggaacacc
     7441 ccatttcccc ttatagtctt tccaatcttc tgtttctatt ttgttgttgt tcttatcaag
     7501 gatatattca ccattcctat ccctttgata ctgttcaggc acttccgcaa aataactgct
     7561 atgcgaatag caaaatccct tacaagctcc ttttgttgtc tcattatttc ctagcaagaa
     7621 aggtatccat ttgttcccca caaaatcttt atcatgcacg attgagtagg ctccgctgtt
     7681 ataagtttta ccgtctgcac ccgtataggt atagccgttt ttctgtaaaa catcggcgta
     7741 atcattcttc acatttgtgg ctgtaccata gaaacgtgct tttctgattg gggcaatgcc
     7801 attttgctct ttattgttta cccaatcttt taaggaaacg cttgctgtaa ttcccccacg
     7861 actgtggtta ctggtatcaa ccgaccagcc attacccatt ttttgaacct ctcgataaat
     7921 atcttgattc agtttttctg aattggtttt gggtaaatag ccttggaaca ctttattatt
     7981 taattggtcg taaccgacat acatcagttc agaaacaaga cttgaactga gccataaaga
     8041 atcttttatt ttgttattag aatcagattt atatttccct gttggaggat tcataactgc
     8101 aataacacca ctaccgtttg tactattacg attttgtttt gctgcgttgc ttaatgaatc
     8161 ttctcgatta ttgaaaatac caggattaga cacagtaata actttgccat ttgtgtcttg
     8221 aatgcttgcc agttcttcct tgcttaaatc attcaatgac caaatttgtc tggtatcaaa
     8281 ggatacttta cctgtttttt catctattat cttcttatgg aaccacattt ctttaggtgc
     8341 agttgcagac cttaccgctc tatcggcttg ggttgtggca aacatactcc aagccatatc
     8401 agaactccct gctccccaaa cgagtcctaa tcctgaatcc taggcggtaa tgacgaagga
     8461 tagttttaca aaagtttcgg cctacaattt ataggtttat aataataaaa atatccatca
     8521 aaaaaatgtg atttttcttt ttttaaaagt tgcatcttgc cattcttgta atcacattcg
     8581 taatattcaa catttttcaa atctgaatca aatagatatt gaggtaaaac attcccttcc
     8641 ttatctagtt ctattaattc ctcaaaaact atcttacttt tatgataaaa aacaattttt
     8701 tggaaatctg aattgattat atacagtttt acataatccc aattaaaatt cgttatatca
     8761 ctaattaaga attctttttt attataatta aaacttattt ttcttattac attcttttca
     8821 aatataaaga aaataacaat agtgattaat aaagaaaata tataaagtat atttttattc
     8881 atcttttatc ttctccattg tctggctgaa atttgtgctc tgatttgttt tctcctctgc
     8941 tgaattttaa atccggcacc agtattatct aagtgaatca atatattgct tgtattctgg
     9001 taatcttttt cagataatct ttttcttaca acatcataat ttccattact gtttctttct
     9061 gcttgataaa aaccattatt tttataagtt tccagaatta atccaactaa ctcttttgtg
     9121 tttaacctat tggtatttaa tgctatttct cttcctattt cgttatttag ttgatcaatc
     9181 atttcatctg ctttatctaa tgaaagatta cgtcttatag aataattaat tttttcccca
     9241 ctttcatgac tatttcctac tttaacagca atatctttgc caaattctcg tgtgatggtt
     9301 gcttgccata aaacgtgcct gaatgcattg ccaactccac cctcaccacc aaattcacta
     9361 ttaggaaata aatttaattg aaaagttgaa gcaattgttg aaatattagg ctctaatgta
     9421 gagccaggat ctttatgtac agatccaatt gcaatagcaa ttctagggtg cctaagagca
     9481 aatttcactt gctctacagt atcattattc cccaccgcac tcttcgcatt caggctgcct
     9541 tgcgctgcat ctgttgcgct gttgccgact gccgcacccg tagccgtacc caatacattt
     9601 gtaatcgctg ttacagtctc tttctcttcc gccgttaagt cgcttccttt ttctttgccg
     9661 tataaccatt tgctgatgta aggcgcagcc gcttccgacc cgcccgcact caatgctcct
     9721 gctagagcat tgttgcctcc tgctgcggca accgctgctc ctaataccgc gtgggcaaga
     9781 acgtgagcgg tttcttgact ggcggttagt ttgccattcg cgttttgacc ggctaaatct
     9841 ttaaagtgct gtccaatcgc atacgatacg gctggcgatg cggtagccgc agcgatgccc
     9901 gctccgcttt gggtcggcgc agctaaacct gaggctaaca tgttgagaat gactttgcct
     9961 tgttgccaat tatctgcttt tgctgccgca tcttgagctt catggacttt gcgtttggca
    10021 gtttccatat cgccattggc taatgcctcg gctgctgctg tttcggctgc ttctttgtct
    10081 gctttgagtt tgtctaaatg ttggttaatc tcggtattgg cttgttgaac atttttacta
    10141 aaatcttggc tgacggttct ttgtaaatcc agttcacttt gcaccgcttc tttgttgaag
    10201 gtgttcttca agctgcccga atgtcgttcg gcggtgtctg tggttacgtt tgtatcaata
    10261 tcggctttgg tttgtgccac tgttttgcct gtcagcctga tttgtgcggc ttcgtctgtg
    10321 atttgaatgt tttgggtatt gatgccgctt ttcgtgatgc tgctttgact gtcgctgtcg
    10381 ctgccataac ccactgatga acttgcgctg tttttatcgg ctacgcttgt caggtgtttg
    10441 ttttgaggtt tattttgtgc gccctgtccc agtgttttgc cgcttatgga cgcacttgcg
    10501 cccaatccaa aactttcgcc tttgtattgg ctgtggtttt tgatgtcgct atgggtgagg
    10561 gtggccgtct gaaagcggtt tttacccttg tcttctgcgc tttgggtact ggtgatgatg
    10621 ccgcctttga ggtctgtatg gtttccgacc ttgatttgat agccgtcttc tccggcataa
    10681 ataccgcttt gctcggttac tgaaacatgg tcggctcgga ttttgctttg gctgtaatcg
    10741 ccaccggcac tgaagccata acctacggta acttgtgcac tggcgttttg ttgtttgctt
    10801 tgataggttt cgctatcttg tacgctttga atatgcaagt tttgggcatt aacttgtacg
    10861 cctttgccaa tgacttgcgc gcctttgagt gcggtatcgc cgccgctttg gagaacggtt
    10921 tggctgcctt tatcgccaat atggctgtgg cggtgtgtta cgctgtcgcc attgccgtgg
    10981 cctttgccga cattgccgcc tgcggtaacg cctaatgacc agcctccttg tccgaatgat
    11041 acggcagcac ctgcgttcca acctgatgat ttgttttggc tgcgttcggt attgctttgc
    11101 tcggctgatt ggagtgtgat gtcattatcg gcaatcagga ttgtgcctgc tttgccggca
    11161 atatctgagc ctgcgatgtt gatattggat tgttctgctg cgcctgtggc gattaatgtg
    11221 gttttaccac ctgcttgaat ttgactcgct tgggcttgat tggcttgaac ttgggtggtt
    11281 tgtcggtttt gctgttcgcc gtaggttatg gagatgctga cttgtttggc attggttgta
    11341 ccattggcta agttttgtgc actcttacct gtttgatagg cttgccagcc tgcattggca
    11401 gctgccatgg cattgacacg accatttttg ctttgtccga cttgtttgcc gctttgtgcg
    11461 acggcaatcg cttgttgtgc caaatcggta acgggcgaac tgaatgccac cgttaagcct
    11521 ttttgttcat aggtttggct ggtattactg tttaattggt tgtgtgccgc ttgaatgtct
    11581 atgctttggg catggatggt attgttgcct tccgggctgg aaacggtact gccgatttgt
    11641 tcgtagtgtt tgcctgcaac aatggtggta tcgcctttca ggctgcctac ggtactgcct
    11701 gtatgttcgt tgctttggga ttggttttct tgtgtgtttg tcttgctgcc aatcgtgaag
    11761 ccgatacctg cgctcatcaa tcctgatttt ttggtttgat gataggtttc gctttgatta
    11821 tgggttaggg ttgtgccaat gtgaacatga ttgcctgctt ggatatgggt gccattgtca
    11881 gaaatgacat tgctgccctt tatgttggcg tcgtttcctg cctgcaatac aacttgtttg
    11941 ccgtcaaagg tgctgctttg agcggtttca ttgtggctct gggctttgtc ggtaataacc
    12001 aatttattgc caccgccact tctgcctttg tgtttggacg catcatcaac atgggtcgta
    12061 ttgatgcctg cgctgatgtt gatgtcattt ttggcagaca cagcgagtgt accgtttgcg
    12121 ctgccgactt cggcagcttt ggcattgagg ttattccctg acaatagggc aacatcgcct
    12181 tttgtttgaa tgctgctgcc gacttcgttc gttgaaccgc gaataacatg gttatcggca
    12241 tcaaaatggg ttgcttgatg tttgccggtt tgtacggtat ccagattgat gttgcgccct
    12301 gcttgcagct gggtttgtcc ttgctctgat tgattgctga tttgactggc aatgatgttg
    12361 atgtccttac cagcttgtgc tgctaaaaca cctttttctg tgcctgtgat gtaaatacct
    12421 gctattcggt ctaggtaggt gctgctgcct tgtgtatttt gactgctagc ggcggtgctt
    12481 tggctgttga tgttgttgcc tgcgttgagt aataatgtct gttcggcaga aagaatgccg
    12541 ccaatattat tgatgtcttg tgtggccgta accgctgatt tttgcgcatg aatacgccca
    12601 ccgatattgt ctagcgtatc ggtattgata ataagcgcat tgcgccctgc aatcgtgcct
    12661 gagtttttca ggctgcctga aacattgatt tgtgtattgc tgcctgacaa caatgcacct
    12721 ttaccgtcta tgtcgccatt cttaacgcgt acataaacct gtggcaccaa tacggtttgt
    12781 gtgccgccat caggaagttt aacttctttt tgtaccaacc aaacaatatc gctggtcagt
    12841 tgcgctactt gctcggcact taatgcaatg ccaacgctga gattcatgga gcgtgccgca
    12901 gtcgcgccat tatccattaa ggctttaaat tgttcttcgt cgttttgata accgtctaaa
    12961 cgacgatgcc ctgtcagctc tgcgatctgt tcattgatta aacgttgctc gtaataacca
    13021 tcacccaaac gtttatgtaa attgtttggg tctagtttga ggctgtccag catatagtca
    13081 ctacccaacc attgacggta gttggcaaag cgtggatcgg tttcaacaag atagccttta
    13141 ttgacaggat tgataatgta taagctgctg ctgggtaatg gggtaaaaga attggacgta
    13201 tagggtagcg aaataccgtt gctttgcagc aactcagtgc cttggctggg cgcatgatgg
    13261 cttaatgctt tgcgatgcga ttcataggca aatgaaccca gtgaaatgtt gcgtgtgatt
    13321 tcctccggca aagtgtaatt ttgttcgcta tgtcccgttg agtctcgtcc tttatgtttc
    13381 tcacgccaat agctgtgtaa tttgccattt tcactgaata ctttcttttc gccaaaggtt
    13441 tgctcgttat gcaaaccgtc tttttctgtt tgcacaagca aattgccgcc ggcgatgatt
    13501 tggctatcgg tattaaatac ttctttacca tcaatggtta aatcattacc tgaaatgatt
    13561 ttggctggcg cagtttgggt aacttgggtt ttttgggtga cttgttcata atcgtattta
    13621 tgccaatttt catgcgccgc tctatcaggg gtgcgtaagt ggtctgattc atcgttatag
    13681 acagaccagc ctaattcatg ttgcgtgcct tcccgcaata attcgtgtcg tccaaatgct
    13741 tcgtaatcaa caatatgctc gcgccctgtt tctaccaact gcgttttcaa atgctcattg
    13801 gtattgtgca gcttttctac acctaaacgc attttgccgg cggcttcaat ggtggcactg
    13861 gcattgcgta tgctttgggc tttgcctgtg gcttggccat tggtatctaa tgcgccgcca
    13921 accgccatat cgttaccgct gtaaatcaga ctgttttcac ggttatttaa ttgtccaatg
    13981 cctaaattca ggttttcgcg tgccgcaatg gcggcacctg taccgttttc atcttgattg
    14041 tctaagcggg tagccgcaat agcgatattg tcaccataaa tccgacctgt accgatatta
    14101 ttcacttgcc cggcttggat tttggtttgt tgtccgtcaa tcaagcctct attggttaaa
    14161 ttgtgctgcg tgccaatgtc tgtcgtaccg ccggattgaa tgttgccttg tgctgcatta
    14221 tcaaggttat ttgctttaat ccgaatgcgt tttcctgctt gcaaggtatg tgaatttttc
    14281 aggctgcctc gtgtactgag cgacaattca ttgcccgcca cgatcttgcg tcctacataa
    14341 aaatcatctt gtaacgcaat atccagttta ttatcagcgg caagtgtgcc gttgttggat
    14401 aacgattttg cctgaatagc aacatcacgg cctgattgta tcgtgccatt cgtattatca
    14461 atggcagcgg tagattgttg accatcgtga ataatcagtt gttgattggt cgctatttcg
    14521 ccattttggt tgttcaggct gcctgaaacg gctaaatccg ctatttctgc tgataataac
    14581 ttgccatgag cgttatccag ttgaccggtt tcaatctcta actgttggcg tgttgtgatg
    14641 ttgccatttt gattattcag gctgccggct tgaatgtgga ccgcatcact gataattgtt
    14701 ccattgtgat tgtcaaacgc cgaacctttt gcatttaact gatgaatgtc tatttgtcct
    14761 gcattattta aaccttgttg cgcactaaca tctgtttgac cattggcaat aatactgcct
    14821 gaattatcca gtgcaccatg agtgcgaatt gtcccatcag caaaggtagg cgcagttatg
    14881 tttgatatag aaacggttgc agtacccgta cctgttgccg ttgttggtgt ggtagtggat
    14941 gaatgggaag atgcattgta actattgccg gtttgattgc ttgaaccatt tgacgcggtt
    15001 ggtgcggtat cttgtaaacc catgcggcca cggttatcca ttttgccttg tgcatcaata
    15061 tggagttttt gtgaacctgt ttgagagagt ttgccttgat tattaagtgt gtcggtatca
    15121 atagccaaac gagcggcttc aatggtgcct gatgtttcat ttttcaggct gcccgaattg
    15181 tgaatcaata tttcgcctga ggacaataat gtgccagtgt tttgaatcga ctgactgtga
    15241 atttgagtgc cttgttgcga taccgccgta ccgctgtttt caacgccctg actgcggata
    15301 ttgactttgt gttccgctgt attatccgta tctttcgcat tggcggcagc catcgtgcca
    15361 ctattgacta aacggccatt tgcatcaatc gccacattac cggaagaagc aaacaactgc
    15421 ccttgattac gaatgcctgc ttgctcggcc gtactgatca aggtgatttt gttggcatac
    15481 atacctccta atttgcctgt atcaatcgca aataaaggga tatgtgtgcc gttgttggct
    15541 gtattgtttg acgtattggc agcagcatta ttgagaatag gcgaatgtgc attacctgtt
    15601 gcgaccacat cgttttgtcc cgcgacgaca cgaacatctt gtccccatac gggtgcatca
    15661 attttggaat gataactgag aatacgtgtg aaatcggtat cacgggcatc caaaccttgt
    15721 cccgtgatcg caacatgacc cgaacgaacc tgaaagcctg ttaatgc
//
LOCUS       DAWHSK010000018        15399 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.018, whole genome shotgun sequence.
ACCESSION   DAWHSK010000018 DAWHSK010000000
VERSION     DAWHSK010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15399
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.018"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..1350)
                     /locus_tag="WIC03_000418"
     CDS             complement(<1..1350)
                     /locus_tag="WIC03_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEZ3882973.1"
                     /translation="MLIKSEYKPRMLPKEEQVKKPMTSNGRISFVLMAIAVLFAGLIA
                     RGLYLQTVTYNFLKEQGDNRIVRTQTLPATRGTVSDRNGAVLALSAPTESLFAVPKEM
                     KEMPSAAQLERLSELVDVPVDVLRNKLEQKGKSFIWIKRQLDPKVAEEVKALGLENFV
                     FEKELKRHYPMGNLFAHVIGFTDIDGKGQEGLELSLEDSLHGEDGAEVVLRDRQGNIV
                     DSLDSPRNKAPKNGKDIILSLDQRIQTLAYEELNKAVEYHQAKAGTVVVLDARTGEIL
                     ALANTPAYDPNRPGRADSEQRRNRAVTDMIEPGSAIKPFVIAKALDAGKTDLNERLNT
                     QPYKIGPSPVRDTHVYPSLDVRGIMQKSSNVGTSKLSARFGAEEMYDFYHELGIGVRM
                     HSGFPGETAGLLRNWRRWRPIEQATMSFGYGLQLSLLQLARAYTALTHDGVLLPVSFE
                     "
     gene            complement(1411..1674)
                     /gene="ftsL"
                     /locus_tag="WIC03_000419"
     CDS             complement(1411..1674)
                     /gene="ftsL"
                     /locus_tag="WIC03_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEZ3882974.1"
                     /translation="MNKLNFLLLLAVCVSAFSVVMQQNQYRLNFTALDKAKKQEIALE
                     QDYAQMRLQQARLANHEAIRAAAEKQNLHPPVSGNTFMVAHQR"
     gene            complement(1671..2657)
                     /gene="rsmH"
                     /locus_tag="WIC03_000420"
     CDS             complement(1671..2657)
                     /gene="rsmH"
                     /locus_tag="WIC03_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEZ3882975.1"
                     /translation="MSGAESYRHITVLLNEAVDALAVREDGVYVDGTFGRGGHSRLIL
                     SRLGDAGRLIVFDKDPQAIAVAEELARSDKRVGVVHGGFASFQTALDGLGIGKVDGAL
                     FDLGISSPQIDDGSRGFSFRFDAPLDMRMDTTRGMSAAEWIAVASEQDLHEVIKNYGE
                     ERFSRQIARAIVAQRAESPIDTTRKLAQIVAQNVRTRERGQDPATRTFQAIRIFINRE
                     LEEVGAVLPQVMCRLKEGGRLAVIAFHSLEDRIVKQFVKKYSQHAPLPRWAAVREADL
                     PEPPLKIAGRALKPGEAEIAANPRARSAVLRVAERTAGPIPEQSQRKTSEWQ"
     gene            complement(2654..3109)
                     /gene="mraZ"
                     /locus_tag="WIC03_000421"
     CDS             complement(2654..3109)
                     /gene="mraZ"
                     /locus_tag="WIC03_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEZ3882976.1"
                     /translation="MFGGAHELSIDSKGRLAVPAKFRDILSRLYTPAVVVTLESKHKL
                     LMYPVAEWEKVAAQLLNLKVADNPVLRRFQNLLLHNAEILEWDSAGRVLLPAGLRKRV
                     DFDREVVLVGRANRLELWGREQWEAEMVQALDDDPDELAFQLGQTDLQL"
     gene            3388..4605
                     /locus_tag="WIC03_000422"
     CDS             3388..4605
                     /locus_tag="WIC03_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ3882977.1"
                     /translation="MTAPVPTCNMSGNPINLHKLTLIMPLPLPSPEARQFSLKLQTLI
                     AEKIGKHGNWIPFSRFMELVLYAPQYGYYTGGSHKIGNTGDFITAPTLTSLFAQTLAR
                     QLQELLSQTAGNIYEFGAGTGQLAADLLGSISDGISRYYIIEISPELAARQKNLIQAR
                     APEASQKVVHLTALPEAFDGIIIGNEVLDAMPVEIVRKDEGGSFEHVGVCTDNGRFAY
                     SARPLHDPSLSTSASLYFPQTDYPYTSELHPQQYAFIRTLASRLEHGCMIFIDYGFDA
                     AQYYHPQRNQGTLIGHYRHHVIHNPFDFIGLADLTAHVNFTDIAQAGTDAGLDLIGYL
                     PQSHFLLNLGITELLAQTGKTNSAAYIREAAAVQKLIDQHEMGELFKVIAFGKNIGID
                     WAGFRFGDICHKL"
     gene            4737..4813
                     /locus_tag="WIC03_000423"
     tRNA            4737..4813
                     /locus_tag="WIC03_000423"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4771..4773,aa:Met,seq:cat)
     gene            4821..4896
                     /locus_tag="WIC03_000424"
     tRNA            4821..4896
                     /locus_tag="WIC03_000424"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4854..4856,aa:Ala,seq:ggc)
     gene            complement(5187..6008)
                     /locus_tag="WIC03_000425"
     CDS             complement(5187..6008)
                     /locus_tag="WIC03_000425"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEZ3882978.1"
                     /translation="MDFLIVLKALMMGLVEGFTEFLPISSTGHLIVFGNLVGFHSNHK
                     VFEIAIQLGAVLAVVFEYRQRFSNVLHGLGKDRKANRFVLNLAIAFIPAAVMGLLFGK
                     QIKEYLFNPLSVAVMLVLGGFFILWVEKRQSRAEPKIADVDALRPIDALMIGIAQVFA
                     LIPGTSRSGSTIMGGMLWGIERKTATEFSFFLAVPMMVAATAYDVLKHYRFFTLHDVG
                     LILIGFIAAFVSGLVAVKALLKFVSKKNYIPFAYYRIVFGIVIIILWLSGWISWE"
     gene            complement(6042..6686)
                     /locus_tag="WIC03_000426"
     CDS             complement(6042..6686)
                     /locus_tag="WIC03_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEZ3882979.1"
                     /translation="MKLKHLLPLLLSAVLSAQAYALTEGEDYLVLDKPIPQEQSGKIE
                     VLEFFGYFCVHCHHFDPLLLKLGKALPSDAYLRTEHVVWQPEMLGLARMAAAVNLSGL
                     KYQANPAVFKAVYEQKIRLENRAVAGKWALSQKGFDGKKLMRAYDSPEAAAAALKMQK
                     LTEQYRIDSTPTVVVGGKYRVIFNNGFDGGVHTIKELVAKVRAERKLSNHPVQK"
     gene            complement(6689..7534)
                     /gene="ftsN"
                     /locus_tag="WIC03_000427"
     CDS             complement(6689..7534)
                     /gene="ftsN"
                     /locus_tag="WIC03_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HEZ3882980.1"
                     /translation="MNKFSQSGKGLSGFFFGLILATVIIAGILFYLNQSGQNAFKIPV
                     PSKQPAETEILKPKNQPKEDIQPEPADQNALSEPDAATEAEQSDAEKAADKADEVEEK
                     AGEPEREEPDGQAVRKKVLTEEREQTVREKAQKKDAETVKKQAVKPSKETEKKAPKEE
                     KKAEKEKVAPKPTPEQILNSGSIEKARSAAAKEVQKMKTSDKAEATHYLQMGAYADRR
                     SAEGQRAKLAILGISSKVVGYQAGHKTLYRVQSGNMSADAVKKMQDELKKHEVASLIR
                     SIESK"
     gene            complement(7640..9136)
                     /locus_tag="WIC03_000428"
     CDS             complement(7640..9136)
                     /locus_tag="WIC03_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEZ3882981.1"
                     /translation="MSLALVYSRALSGMNAPLVEVEAHLANGLPHFNIVGLPDTEVKE
                     SRDRVRAAIIQSGFEFPAKKITVNLAPADLPKESGRFDLPIAIGILAASGQVAPEKLA
                     EYEFAGELALSGLLRPVRGALAMAWQGMQAKRAFVLPEENAGQAAVMRGITVYGARSL
                     GEVAAHLNGIEPLAQTECSVPQMPSEHGGQPDLCDVKGQHTARLALEIAAAGGHSLLM
                     MGPPGTGKSMLSQRLPGILPPLTEDELVEVWALRSLLPNHQQQLDSNRPFRSPHHSAS
                     AAAMVGGGSDPRPGEISLAHHGVLFLDELPEFDRKVLEVLREPLENGEIHISRAARQA
                     VYPAKFQLVAAMNPCPCGYLGHPIKPCRCTPESVARYRSKISGPLLDRIDLTIEVPSL
                     SAAELMQQEAGESSASVLERVIAARDKQYARQGKVNAALSVSELDSQARIQKEAQEAL
                     GGLLEKLSLSARSFHRIMRVARTLADLAGDEEVGRSHVMKAIGFRRAL"
     gene            complement(9150..9470)
                     /locus_tag="WIC03_000429"
     CDS             complement(9150..9470)
                     /locus_tag="WIC03_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003763371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HEZ3882982.1"
                     /translation="MFGKQLFEEVGSKISETIANSPAKDVEKNIKAMLGGAFNRMDLV
                     TREEFDIQQQVLIKTRTKLAALEARLAKLEAAQNPERAALEAAEAAAEEAVAEIRQQT
                     EAGE"
     gene            9953..11479
                     /gene="putP"
                     /locus_tag="WIC03_000430"
     CDS             9953..11479
                     /gene="putP"
                     /locus_tag="WIC03_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HEZ3882983.1"
                     /translation="MNPMYITFAIYLVAVLLIGLAAYFSTRNFDDYILGGRSLGPFVT
                     AMSAGASDMSGWLLMGLPGAIYLSGLNEAWIAIGLLVGAYFNWLLVAGRLRVHTEYAN
                     NALTLPDYFFHRFGAGGHLMKVVSALIILFFFTIYCASGIVAGATLFQSLFEGMTYNQ
                     AMWLGAGATIAYTFLGGFLAVSWTDTLQASLMIFALILTPVMVYLGLGGAEQMSAAIQ
                     SVAAGTGKEYGSLFAGTTVIGIISTAAWGLGYFGQPHILARFMAAESAKSLVSARRIG
                     MTWMALCLAGAVAVGYFGIAYFGANPDKVSSMSGNHERIFIALSTLLFNPWIAGIILS
                     AILAAVMSTLSCQLLVCSSAITEDFYKGFLRKNAQQSELVWVGRLMVLAIAVISILIA
                     SDPNSKVLGLVSYAWAGFGAAFGPIVILSVLWKRITAYGALSGMVAGALTVVVWAEWV
                     KKPAQAAGESGLLTMYEIVPGFIVCLIVAVLVSLFNKEPSRETQERFEKADADYRAAR
                     "
     gene            11730..15335
                     /gene="putA"
                     /locus_tag="WIC03_000431"
     CDS             11730..15335
                     /gene="putA"
                     /locus_tag="WIC03_000431"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HEZ3882984.1"
                     /translation="MFHFAFPAQTALRQAITDAYRRNEIEAVQDMLQRAQMSDEERNA
                     ASELARRLVTQVRAGRTKAGGVDALMHEFSLSSEEGIALMCLAEALLRIPDNATRDRL
                     IADKISDGNWKSHLNNSPSLFVNAAAWGLLITGKLTATNDRQMSSALSRLISKGGAPL
                     IRQGVNYAMRLLGKQFVTGQTIEEALQNGKEREKMGYRFSFDMLGEAAYTQADADRYY
                     RDYVEAIHAIGKDAAGQGVYEGNGISVKLSAIHPRYSRAQHGRVMGELLPRLKELFLL
                     GKKYDIGINIDAEEANRLELSLDLMEALVSDPDLAGYKGIGFVVQAYQKRCPFVIDYL
                     IDLARRNNQKLMIRLVKGAYWDSEIKWAQVDGLNGYPTYTRKVHTDISYLACARKLLS
                     AQDAVFPQFATHNAYTLGAIYQMGKGKDFEHQCLHGMGETLYDQVVGPQNLGRRVRVY
                     APVGTHETLLAYLVRRLLENGANSSFVNQIVDENISIDTLIRSPFDTIAEQGIHLHNA
                     LPLPRDLYGKCRLNSQGVDLSNENVLQQLQEQMNKAAAQDFHAASIVNGKARDVGEAQ
                     PIKNPADHDDIVGTVSFADAALAQEAVGAAVAAFPEWSATPAAERAACLRRFADLLEQ
                     HTPALMMLAVREAGKTLNNAIAEVREAVDFCRYYANEAEHTLPKDAEAVGAIVAISPW
                     NFPLAIFTGEVVSALAAGNTVIAKPAEQTSLIAGYAVSLMHEAGIPTSALQLVLGAGD
                     VGAALTNDARIGGVIFTGSTEVARLINKALAKRGDNPVLIAETGGQNAMIVDSTALAE
                     QVCADVLNSAFDSAGQRCSALRILCVQEDVADRMLDMIKGAMDELVVGKPIQLTTDVG
                     PVIDAEAQQNLLNHINKMKGVAKSYHEVKTAADVDSEKSTFVRPILFELNNLNELQRE
                     VFGPVLHVVRYRADELDNVIDQINSKGYALTHGVHSRIEGTVRHIRSRIEAGNVYVNR
                     NIVGAVVGVQPFGGHGLSGTGPKAGGSFYLQKLTRAGEWVAPTLSQIGQADEAALKRL
                     EALVHKLPFNAEEKKAAAAALGHARIRTLRRAETVLTGPTGERNSISWHAPKRVWIHG
                     GSTVQAFAALTELAASGVQAVVEPDSPLASYTADLEGLLLVNGKPETVGISHVAALSP
                     LDSARKQELAAHDGALIRVLPSENGLDILQVFEEISCSVNTTAAGGNASLMAVAD"
BASE COUNT         3595 a         4520 c         3729 g         3555 t
ORIGIN      
        1 ttcaaagctg accggcagca aaacgccgtc gtgcgtcagt gcggtatagg cgcgcgccaa
       61 ttgcagcagg ctcaattgca ggccgtaacc gaaagacatc gtcgcctgtt cgatgggccg
      121 ccacctgcgc caatttctca acaaacctgc agtttcgccc ggaaagcccg agtgcatacg
      181 cacaccgatg cccaactcat gatagaagtc atacatttct tcggcaccga aacgcgcaga
      241 cagtttgctt gtgccgacgt tggacgattt ctgcatgatg ccgcgcacat ccaaagaggg
      301 gtaaacatgg gtatcgcgca cgggagacgg tccgatttta taaggctgcg tattcagccg
      361 ttcgttcaaa tcggttttgc ccgcatccaa tgctttcgca atcacaaacg gtttgattgc
      421 cgaaccgggt tcgatcatat cggttacggc acggttgcgc cgctgttcgc tgtctgcccg
      481 gccgggcctg ttgggatcgt aggcgggcgt attggccaag gcgaggattt cccccgtgcg
      541 ggcatccaaa accaccaccg ttccggcttt tgcctgatgg tattcgaccg ccttgttcaa
      601 ctcttcatag gccaaggtct gaatcctctg atcgagggaa aggatgatgt ctttgccgtt
      661 tttcggggct ttattgcgcg gggagtccaa gctgtccaca atattgccct gccggtcccg
      721 caaaacgact tccgcgccgt cttcgccatg caggctgtct tcaagcgaaa gttccaaacc
      781 ttcctgacct ttgccgtcaa tatcggtaaa tccgatgacg tgtgcaaaca ggttgcccat
      841 cgggtaatgg cgttttaatt ctttttcaaa tacaaagttt tccaaaccca aggctttgac
      901 ctcttcggca accttgggat cgagctgccg cttaatccag ataaacgact tgcctttctg
      961 ttcgagcttg ttcctcaaaa catcaaccgg cacatcgaca agctcggaca ggcgttccaa
     1021 ttgtgcggca gacggcattt ccttcatctc tttaggcacg gcaaacaggg actccgtcgg
     1081 cgcactcaac gccaaaaccg caccgttccg gtccgaaacc gtaccgcgtg tagccggcaa
     1141 tgtttgagtc cgcacaatcc ggttgtcgcc ctgttctttc aaaaagttat acgttaccgt
     1201 ctgcagatac agtccgcggg caatcagacc ggcaaacaag accgctattg ccatcaggac
     1261 gaagctgatc cgtccgttac tggtcatcgg ctttttgacc tgctcttctt tgggcagcat
     1321 ccggggctta tattcactct taatcaacat ttttacttct cgttattatt atcctgacgc
     1381 aggaatccga ttccggcaca caggctgctt ctatctttga tgcgccacca taaaggtatt
     1441 gcccgaaacc ggcggatgga ggttttgttt ttctgccgcc gccctgatcg cttcgtggtt
     1501 cgccaaacgc gcctgttgca gcctcatttg cgcataatcc tgctccaagg cgatttcctg
     1561 ttttttcgcc ttatccaaag ctgtgaagtt gagcctgtac tggttttgct gcatcacaac
     1621 ggaaaaagcg gaaacgcaca ccgcaagcag cagaaggaaa ttcaatttgt tcattgccat
     1681 tcagacgttt ttctctgtga ttgttccggt atcggaccgg cagtccgctc cgccacacgc
     1741 aaaaccgcac ttctcgccct cggattggcg gcaatttccg cctcacccgg ctttaatgcc
     1801 ctgcccgcga ttttcagggg cggctcgggc aaatccgctt ccctgaccgc cgcccagcgc
     1861 ggcaggggcg cgtgttgcga atattttttg acaaactgct tcacaatgcg gtcttccaac
     1921 gaatggaaag caatgaccgc caaacgtccg ccctctttca gacgacacat gacctgcggc
     1981 aatactgccc ctacttcttc aagctcgcgg ttaataaaga tgcggattgc ctggaaggtg
     2041 cgcgtcgcag gatcctgccc ccgctcgcga gtacggacgt tttgtgccac gatctgcgcc
     2101 agcttgcggg ttgtatcgat tggactttcc gcccgttgcg caacaatggc gcgcgcaatc
     2161 tggcggctaa accgctcttc accataattc ttgattacct cgtgcaaatc ctgttccgac
     2221 gcaaccgcta tccactctgc ggcagacata ccgcgcgtcg tatccatacg catatccaaa
     2281 ggggcatcga aacggaagct gaaaccgcgg ctgccgtcat cgatttgcgg ggacgaaatc
     2341 cccaaatcaa acagcgcacc gtccaccttg ccgataccca aaccgtccaa tgccgtctga
     2401 aacgaagcaa aaccgccatg cacgacaccg acccgtttgt ccgaacgcgc cagctcttct
     2461 gccacagcaa tcgcctgcgg atctttgtcg aaaacaatca accgccccgc atcgcccaaa
     2521 cgcgacaaaa tcagccggga atgccctccc ctgccgaacg taccgtccac atagacaccg
     2581 tcttcgcgca cggcaagcgc atccaccgcc tcattcagca agaccgtgat atgccggtaa
     2641 ctttctgctc cactcacaat tgcaaatccg tctgacccaa ctggaaggca agttcgtcag
     2701 gatcgtcatc caaagcctga accatctcag cctcccactg ctcgcgaccc caaagctcca
     2761 aacggttggc acgaccgacc aaaacgactt cacggtcgaa atccaccctt ttcctcagtc
     2821 cggcaggaag cagcacccgg ccggcgctgt cccattccaa aatttccgcg ttatgcagca
     2881 aaagattttg aaaccgccgc aaaacagggt tatccgccac ttttaagttt aaaagttgcg
     2941 ccgcaacctt ttcccactcc gcaacagggt acatcaacag cttgtgtttc gactcgagcg
     3001 ttaccactac ggcaggcgta tagaggcgcg acagaatgtc acggaatttg gcaggaacag
     3061 ccaaccgccc cttactgtcg atgcttaatt cgtgtgcgcc gccgaacatg acatgtccca
     3121 agccgaaatc aaaatcacaa gggtaaaaga gacactttgc cccacaattc cccaccaatc
     3181 gacactataa gaaattttaa acactcggtc aaatcagggc atgaaaactc attaacatat
     3241 ctgaaatttt tattcctttt aaaaacaata agataaaaaa tgacgacaac ggcacggcgg
     3301 gtgcggtaca gaataatcga accaataaac aactatatat gattaattta ataatataaa
     3361 cacaatatat agtattaaga taaagccatg acagcacccg taccaacgtg taatatgtcg
     3421 ggaaatccaa taaatttaca caagctaaca cttatcatgc ccctccccct cccctctccc
     3481 gaagcacggc aattctcgct caaactgcaa accctcattg ccgaaaaaat cggcaaacac
     3541 ggcaactgga ttccattttc acgttttatg gaattggttt tatacgctcc gcaatacggc
     3601 tactacaccg gcggcagcca taaaatcggc aataccgggg attttattac cgcaccgacc
     3661 ctcacctctc tgtttgcaca gacactggca cgccaacttc aagaacttct atcccaaacg
     3721 gcgggcaata tctatgaatt cggcgcggga accggacagc ttgccgccga tttgttgggc
     3781 agcatttcgg acggcatcag ccgttactat attattgaaa tatcgccgga gctggcagca
     3841 cgtcagaaaa acctgattca agcccgcgca ccggaagcat ctcaaaaagt tgtccacttg
     3901 accgcacttc ccgaagcgtt tgacggcatc atcatcggca acgaagtact cgatgccatg
     3961 cctgtcgaaa tcgtccgtaa agatgaaggc ggctcattcg agcatgtcgg cgtttgcacg
     4021 gacaacggcc gtttcgccta ttctgcaaga cccttgcacg acccgtcgct ttccacctcg
     4081 gcttccctct attttcctca aacagattat ccctacacca gcgaactaca tccgcaacaa
     4141 tatgccttta tccgcaccct tgcctcaaga ctcgaacacg gctgcatgat attcatcgac
     4201 tacggttttg atgcagcgca gtattaccac ccgcaacgca atcaaggtac tctaatcgga
     4261 cactaccgac atcacgttat ccacaatcct tttgacttca tcggattggc cgacctgacc
     4321 gcgcatgtca actttaccga cattgcccaa gccgggacgg atgccggatt agatttgata
     4381 ggttaccttc cccaatccca tttcttattg aacctgggca ttaccgagct attggcacag
     4441 acggggaaaa cgaattcggc agcctatatc cgtgaagctg ctgccgttca gaaactgatt
     4501 gaccagcacg aaatgggcga actgtttaaa gtcatcgcat tcggaaaaaa tatcggcatc
     4561 gactgggcag gattccgctt cggcgacatc tgccacaaac tctaaccctc atgccgcctg
     4621 aatccgcttc agacgacata aactttttaa catttaaaaa cagtcaacta attcaaaatt
     4681 aaaaaatacg gcttgtcaaa aaaacagaaa aacatataat agcgtcttca cgaaacggcg
     4741 aattagctca gtcggttaga gcagaggaat cataatcctt gtgtccgggg ttcgagtccc
     4801 tgattcgcca ccaaattttc gggggtatag ctcagttggt agagcgcttg catggcatgc
     4861 aagaggtcag cggttcgatc ccgcttacct ccaccagata aaaaagcaca gaccgtaaaa
     4921 aggtatgtgc ttttttatta cctgattacc ggcaaataaa gaataaacca ctgccttcaa
     4981 aacaggcaat cgactttaaa cctatcgccc cgcctgtcct gattttatag tgaattaaat
     5041 ttaaaccggt acagcgttgg ctcgccttgc cgtactatct gtactgtctg cggcttcgtc
     5101 gccttgtcct gatttttgtt aattcactat atcagcccgc cagacaaacc cgacccgaat
     5161 aatgtcttca ggtcgggttt atggtttcat tcccaactta tccagcccga caaccacaat
     5221 attatgatga cgatgccgaa aacaatgcgg taataggcaa acgggatata gtttttcttg
     5281 gaaacaaact tcagcagtgc tttgaccgct actaaaccgg aaacaaaagc ggcaataaag
     5341 cctatcagaa tcaaaccgac atcatgcagg gtgaaaaacc ggtaatgttt caggacatca
     5401 taagccgttg cggcaaccat catcggaacg gccaagaaaa atgaaaactc cgttgccgtt
     5461 ttccgctcga ttccccaaag catcccgccc ataatcgtac tgcccgagcg ggatgttccc
     5521 ggaatcaagg caaacacctg cgcgataccg atcatcaagg catcaatcgg acgcaatgca
     5581 tcaacatcgg caattttagg ctctgctcgg ctttggcgtt tctccaccca caaaataaaa
     5641 aaaccgccca aaaccagcat gactgcaaca ctcaaggggt taaacagata ctctttgatt
     5701 tgtttgccga acaacagccc catcacggcg gcaggtataa aagcaatggc aagattgagg
     5761 acgaaacggt tggctttccg gtcttttcct aagccgtgca acacattgct gaaacgctgc
     5821 cggtattcaa acactactgc caaaaccgca ccaagctgga tggcaatttc aaaaaccttg
     5881 tggttgctgt gaaaaccgac caagttaccg aacacaatca aatgtccggt gctggaaata
     5941 ggtaaaaatt cggtaaaacc ttctaccaag cccatcatca gggctttcag gacaatcaga
     6001 aaatccattg cttggtttct ttcaaatgcg cggggatgcg gttatttttg cacaggatgg
     6061 tttgacagtt tgcgctccgc cctgactttg gcaaccaatt ctttaatcgt atgaacgccg
     6121 ccgtcaaagc cgttattgaa gataacgcgg tattttccgc cgacgacaac ggtcggcgtg
     6181 ctgtcgatgc ggtattgttc cgtcagtttc tgcattttta atgcggcggc ggcagcttcg
     6241 ggggaatcat aggcgcgcat cagttttttg ccgtcaaagc ctttttgaga caaagcccat
     6301 tttccggcaa cagccctgtt ttccaagcgg attttttgtt cgtaaactgc tttaaacacg
     6361 gcagggtttg cctgatattt caaacccgac aaattgacgg cagccgccat tcttgccaga
     6421 ccgagcattt caggctgcca gaccacgtgc tccgtcctca aataggcatc agacggcaat
     6481 gccttgccca gtttcaataa caaaggatcg aaatgatggc aatgtacgca gaaatagccg
     6541 aaaaattcca aaacctcaat tttacccgac tgttcttgag gaatgggttt atccaacaca
     6601 agatagtctt ccccttccgt cagggcatat gcctgcgcgg acaacactgc cgacagcagc
     6661 agcggcaaca gatgtttgag cttcataatt atttgctttc gatagaacgg atcaggctgg
     6721 cgacttcatg ttttttcaac tcgtcctgca tttttttcac cgcatcggca gacatattgc
     6781 cgctttgcac ccggtaaagc gttttatgtc ccgcctgata accgaccacc ttggaagata
     6841 tgcccaagat tgccagtttg gcacgctgcc cttccgcgct ccggcggtcg gcatacgcgc
     6901 ccatttgcag ataatgcgtt gcttccgcct tgtcggacgt tttcattttc tgcacttctt
     6961 tggcggcggc actgcgcgct ttttcgatgc tgccgctgtt gaggatttgt tccggggtcg
     7021 gtttgggtgc aactttttcc ttttccgcct ttttctcttc ttttggagct tttttctctg
     7081 tttctttaga cggttttacc gcttgttttt taaccgtttc ggcatctttc ttctgcgctt
     7141 tttccctgac ggtttgttca cgctcttccg tcagcacttt cttacgcact gcctgtccgt
     7201 ccggctcttc ccgttccggc tcgcccgcct tttcttcaac ctcgtcggct ttatcggcag
     7261 ctttttccgc atccgactgc tctgcctctg tcgcagcatc cggttcggac aaggcgtttt
     7321 gatcggccgg ttcaggttgg atgtcttcct taggctggtt tttcggtttc aggatttccg
     7381 tttctgcagg ctgcttcgac ggaaccggga ttttgaacgc attttgaccg ctctggttca
     7441 gataaaacaa aataccggca ataatgaccg tcgccagtat caaaccgaag aaaaaaccgg
     7501 acagaccttt tccggattgg gaaaatttgt tcataaacat accttaatgt gtttcagacg
     7561 gcattaacgc cgtttgctta cgggcggata ttctaacaat atcgccatat ttgggcaaaa
     7621 cctgcttcca ttcccgttcc tataaagcgc gacggaaacc tatggctttc atgacgtggc
     7681 ttctgccgac ttcttcgtcg cccgccaaat ccgccaatgt acgcgccacg cgcataatgc
     7741 ggtggaagct gcgggcggaa agggagagtt tttccagcag gccgcccaat gcttcctgcg
     7801 cttctttttg aatgcgggct tgggaatcga gttcgctgac gctcaaagcg gcattcactt
     7861 tgccttgccg tgcgtattgt ttgtctctgg cggcgataac gcgttccaaa acggacgcgc
     7921 tgctttcccc tgcttcctgc tgcatcagtt cggcggcgga caggctcggg acttcgatgg
     7981 tcaaatcgat gcggtcgagc agcgggccgg aaatcttgct gcggtaacgc gcgacgcttt
     8041 cgggcgtgca acggcagggt ttgatgggat gcccgagata accgcaagga caagggttca
     8101 tggcggcaac aagttggaat ttggcaggat agacggcttg gcgcgccgcg cgggaaatgt
     8161 ggatttcgcc gttttccaac ggttcgcgca aaacttctaa aactttgcgg tcgaactcgg
     8221 gcagctcgtc caaaaacaat acgccgtggt gcgccaatga aatttcgccc gggcgcggat
     8281 ccgaaccgcc gccgaccata gccgccgcgc tggcgctgtg atgcggactg cggaaaggac
     8341 ggttgctgtc gagttgttgt tggtggttgg gcaggagcga acgcaatgcc caaacttcta
     8401 ccaattcgtc ttcggtcagc ggcggcagga tgccgggcag ccgttgggag agcatagact
     8461 tgcccgttcc cggcggaccc atcatcaaga ggctgtgtcc gcctgcggca gcgatttcca
     8521 aagcaaggcg cgcggtgtgc tgacctttca catcgcacaa atcaggttgt ccgccatgtt
     8581 cagacggcat ttgcggtacg gaacattcgg tttgcgccaa aggttcgatg ccgttcaaat
     8641 gggcggcgac ttcacccaaa gagcgcgcgc cgtaaacggt aatgccgcgc atcacggcgg
     8701 cttgtcctgc gttttcttca ggcaaaacaa atgcacgttt tgcctgcata ccctgccacg
     8761 ccatcgccaa cgcgccgcgc acggggcgca acagccccga cagtgccaat tcccccgcaa
     8821 actcgtattc cgccagtttt tcgggcgcaa cctgccccga tgcggcaagg atgccgattg
     8881 caatcggcaa atcgaaacgc cccgactctt tgggcaggtc ggcgggggcg aggttgacgg
     8941 tgattttttt ggcggggaat tcaaaaccgc tttgaataat ggcggcacgg acgcggtcgc
     9001 gactttcctt tacttccgta tcgggcagcc cgacgatgtt gaaatgcggc aggccgttgg
     9061 caaggtgggc ttccacttcg accaacggcg cattcatacc actcaaggcg cggctgtaaa
     9121 ccaaggcaag cgacatattt cagacggcct tattcgccgg cttcggtttg ctgcctgatt
     9181 tcggcgacgg cttcttcggc agcggcttca gccgcttcca atgctgcccg ttcgggattt
     9241 tgcgcggctt cgagttttgc caaacgcgct tccaaagccg ccagtttggt acgggttttg
     9301 attaaaacct gctgctggat gtcgaattct tcgcgcgtaa ccagatccat acggttgaac
     9361 gcgccgccca gcatcgcctt aatatttttt tccacatctt tggcagggct gttggcgatg
     9421 gtttcgctga ttttcgagcc gacttcctca aaaagctgct tgccgaacat aatctgtatc
     9481 cttcctgaac atatcaaatt caatcggcta ttgtataagg aaaaatgccg tctgaaaacg
     9541 ggcggcggat aatcggcaaa acataccgcg cctcctttgc ggttgctaaa ttacacctca
     9601 aaacacctgc cgcctgaaaa cggatttcat attccgcgca acgcccgatt acaagacact
     9661 acaaaacaat atgctgtttt aaatgatttt tccgacgcgc atcgtttcaa actcggcttt
     9721 ttaagccatt aagtgctttg caaacaacag aaattgggtt atgctgaaac ggattattta
     9781 caatttcata tagttttatt acatatctta ttgtgattga agataattta tccgaatccc
     9841 cctttcgggt atccggattt tccgttgtac ttttattaga aaaacatttc aggcgcaagt
     9901 tgcttgcaat ttcaaagcct gtaattctga agttttagac gaggaatagc acatgaatcc
     9961 catgtacatt acttttgcaa tctatctggt tgcagtcctt ctcatcgggc ttgccgccta
    10021 tttttctacg cgcaatttcg acgattatat tttgggcggg cgcagcctag gcccgtttgt
    10081 taccgcgatg tcggcaggcg cgtccgatat gtccggctgg ctgctgatgg gtctgccggg
    10141 cgcgatttac ctgagcggtt tgaatgaggc ttggattgcc atcggcctct tggtcggcgc
    10201 gtatttcaac tggctcttgg tggcgggccg cctgcgcgtg cataccgaat acgccaacaa
    10261 tgcgctgacg ctgccggatt atttcttcca ccgcttcggc gcgggcggac acttgatgaa
    10321 agtggtttcc gcactgatta tcctgttctt cttcacgatt tattgcgcct cgggcattgt
    10381 ggcgggcgca accctgttcc aaagcctgtt tgaaggtatg acttacaatc aggcaatgtg
    10441 gctgggcgcg ggcgcgacca tcgcctatac cttcttgggc ggtttcttgg cggtcagctg
    10501 gacggatacg ctgcaggctt ctttgatgat tttcgccctg attttaacgc cggtgatggt
    10561 ctatctgggc ttgggcggcg cggaacagat gtctgccgcg attcaaagcg tcgccgcagg
    10621 cacgggcaaa gaatacggca gcctgtttgc cggtacgacc gtcatcggca tcatttccac
    10681 cgccgcatgg ggcttgggct atttcggaca gccgcacatt ttggcgcgct ttatggcggc
    10741 cgaaagcgcg aaatcgctgg tatccgcacg ccgcatcggt atgacttgga tggcgttgtg
    10801 ccttgcgggc gcggtagcgg tcggttattt cggcattgcg tatttcggtg caaatcccga
    10861 caaagtttct tctatgagcg gcaaccacga acgcatcttc atcgcgcttt ccaccctgct
    10921 gttcaaccct tggatcgccg gcattatttt gagcgcgatt ttggcggcgg tgatgtccac
    10981 cctgtcctgc cagcttttgg tttgctccag tgcgattacc gaagacttct acaaaggctt
    11041 tttgcgtaaa aacgcgcaac agtcggaact ggtatgggtc ggccgcctga tggtcttggc
    11101 gattgccgtg atttccatcc tgattgcttc cgatccgaac agcaaagtat tgggactggt
    11161 gtcttatgcg tgggcaggtt tcggtgcggc attcggcccg attgtgattc tgtccgtatt
    11221 gtggaaacgc atcactgcct acggcgcgtt gtcgggtatg gttgcaggtg cgttaaccgt
    11281 agtcgtatgg gcggaatggg tgaaaaaacc ggctcaagcg gcaggcgaaa gcggcttgtt
    11341 gacgatgtac gaaatcgtcc cgggctttat tgtttgcttg atcgttgccg tattggtttc
    11401 cctgttcaac aaagaacctt cgcgcgaaac ccaagaacgc tttgaaaaag ccgacgcaga
    11461 ttaccgcgcc gcccgataaa cccctttcag acggcacggc atcccggttc gtgccgtctg
    11521 aacccgtttc cgggtcaaac acaaataaac cttgcctgca tacaggcctt atttgtgttt
    11581 tacccgtttc attttcaaag aaacggaagc acctaccctg cccgcatttg aagccttgta
    11641 tttcatctgc ggatttatta cgatattacc gtattcagac cgcaccgatg ccgtctgaac
    11701 cccccgaaaa actttggaga atccaaaaaa tgtttcattt tgcatttccg gcacaaactg
    11761 ccctgcgcca agcgataacc gatgcctacc gccgtaatga aatcgaagcc gtacaggata
    11821 tgttgcaacg tgcacagatg agcgacgaag agcgcaacgc cgcctccgag cttgcccgcc
    11881 gtttggttac ccaagtccgc gccggccgca ccaaagccgg cggcgtggat gcgctgatgc
    11941 acgagttttc actctccagc gaagaaggca tcgcgctgat gtgtctggca gaagccctgc
    12001 tgcgtatccc cgacaacgcc acgcgcgacc gcctgattgc cgacaagatt tcagacggca
    12061 actggaaaag ccatttgaac aacagccctt ccctcttcgt caacgccgcc gcctggggcc
    12121 tgctgattac cggcaaactg accgccacaa acgacagaca aatgagttcc gcactcagcc
    12181 gcctgatcag caaaggcggc gcaccgctca tccgccaagg cgtaaattac gccatgcgcc
    12241 tgttgggcaa acagttcgta accggacaaa ccattgaaga agccctgcaa aacggcaaag
    12301 aacgcgaaaa aatgggctac cgcttctcct tcgatatgtt gggcgaagcc gcctacaccc
    12361 aagccgatgc cgaccgctac taccgcgact atgtcgaagc catccacgcc atcggcaaag
    12421 atgcggcagg acaaggcgtt tacgaaggca acggcatttc cgtcaaactc tccgccatac
    12481 atccgcgcta ctcgcgcgcc caacacggcc gtgtgatggg cgaactgttg ccgcgcctga
    12541 aagagctgtt ccttttgggt aaaaaatacg atatcggtat caacatcgat gccgaagaag
    12601 ccaaccgtct ggagctgtct ttggatttga tggaggcact cgtttccgat cccgatttgg
    12661 caggctacaa aggcatcggt ttcgttgttc aagcctacca aaaacgttgt ccgttcgtta
    12721 tcgactacct gatcgacctt gcccgccgca acaaccaaaa actaatgatc cgcctcgtca
    12781 aaggcgcgta ttgggacagc gaaatcaaat gggcgcaagt ggacggcttg aacggctatc
    12841 cgacctacac ccgcaaagtc cacaccgaca tctcctacct cgcctgcgcg cgcaaactgc
    12901 tttccgcgca agacgcggta tttccgcaat tcgccaccca caacgcctac accttgggcg
    12961 caatctacca aatgggcaaa ggcaaagact ttgaacacca atgtctgcac ggcatgggcg
    13021 aaaccctgta cgaccaagtc gtcggcccgc aaaacttagg ccgccgcgtg cgcgtgtacg
    13081 ccccggtcgg cacacacgaa accctgctcg cctacttggt gcgccgcctg ttggaaaacg
    13141 gcgcgaactc gtctttcgtc aaccaaatcg tcgatgaaaa catcagcatc gacacgctca
    13201 tccgcagccc gttcgacacc atcgccgaac aaggcatcca cctgcacaac gccctgccgc
    13261 tgccgcgcga tttgtacggc aaatgccgtc tgaactcgca aggcgtggac ttgagcaacg
    13321 aaaacgtatt gcagcagctt caagaacaga tgaacaaagc cgccgcgcaa gacttccatg
    13381 ccgcatccat cgtcaacggc aaagcccgcg atgtcggcga agcgcaaccg attaaaaacc
    13441 ctgccgacca cgacgacatc gtcggcacag tcagctttgc cgatgccgcg cttgcccaag
    13501 aagcggttgg tgcagccgtt gccgcgttcc ccgaatggag tgcgacacct gccgccgaac
    13561 gcgccgcctg cctgcgccgt ttcgccgacc tgctcgaaca gcacaccccc gcgctgatga
    13621 tgcttgccgt gcgcgaagcg ggcaaaacgc tgaacaacgc cattgccgaa gtgcgcgaag
    13681 ccgtcgattt ctgccgctac tacgcaaacg aagccgaaca caccctgccc aaagatgccg
    13741 aagccgtcgg cgcgattgtc gccatcagcc cgtggaactt cccgctcgcc atctttaccg
    13801 gcgaagtcgt ttccgcattg gcggcaggca acaccgtcat cgccaaaccc gccgaacaaa
    13861 ccagcctgat tgccggttat gccgtttccc tcatgcacga agccggcatc ccgacttccg
    13921 ccctgcaact cgtcctcggc gcaggcgacg tgggtgcggc attgaccaac gatgcccgca
    13981 tcggcggcgt gattttcacc ggctcgaccg aagtggcgcg cctgatcaac aaagcccttg
    14041 ccaaacgcgg cgacaatccc gtcctgattg ccgaaaccgg cggacaaaac gccatgattg
    14101 tcgattccac cgcacttgcc gaacaagtct gcgccgacgt attgaactcc gccttcgaca
    14161 gcgcgggaca acgctgctcc gccctgcgta tcttgtgcgt ccaagaagac gttgccgacc
    14221 gtatgctcga catgatcaaa ggcgcgatgg acgaactcgt cgtcggcaaa ccgattcagc
    14281 tcactaccga tgtcggcccc gtcatcgatg ccgaagcaca gcaaaacctg ttgaaccaca
    14341 tcaacaaaat gaaaggcgtt gccaagtctt accacgaagt caaaaccgct gccgatgtcg
    14401 attccgaaaa atccacgttc gtccgcccca tcctgtttga attgaacaac ctcaacgaac
    14461 tgcaacgcga agtcttcggc cccgtcctgc acgtcgtccg ctaccgcgcc gacgaactcg
    14521 acaacgtcat cgaccaaatc aacagcaaag gctacgccct gacccacggc gtacacagcc
    14581 gcatcgaagg cacggtacgc cacatccgca gccgcatcga agccggcaac gtttacgtca
    14641 accgcaacat cgtcggcgca gtcgtcggcg tacagccctt cggcggacac ggtctgtccg
    14701 gcacaggccc caaagcaggc ggttcgttct acctgcaaaa actgacccgc gccggcgaat
    14761 gggttgcccc gaccctgagc caaatcggac aggcggacga agccgcactc aaacgcctcg
    14821 aagcactggt tcacaaactg ccgttcaacg ccgaagagaa aaaagccgca gcggccgctt
    14881 tgggacacgc ccgcatccgc accctgcgcc gtgccgaaac cgtccttacc ggaccgaccg
    14941 gcgagcgcaa cagcatctca tggcacgcgc caaaacgagt ttggatacac ggcggcagca
    15001 cggttcaagc ctttgccgca ctgaccgaac ttgccgcctc cggcgtacag gcagtggtcg
    15061 aacccgacag cccgctggct tcctacaccg ccgacttgga aggtctgctg ctggtcaacg
    15121 gcaaacccga aaccgtcggc atcagccacg ttgccgccct gtcgcctttg gacagcgcgc
    15181 gcaaacagga acttgccgcc cacgacggcg cactcatccg cgtcctccct tcggaaaacg
    15241 gactcgacat cctgcaagtt tttgaagaaa tctcttgcag cgtcaacacc acagccgccg
    15301 gcggcaacgc cagcctgatg gcggttgccg actgattttg ccaaaaccgc ccgagcgcgg
    15361 cccgtgaacc aatgccgtct gaaaaccttt cagacggca
//
LOCUS       DAWHSK010000019        15258 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.019, whole genome shotgun sequence.
ACCESSION   DAWHSK010000019 DAWHSK010000000
VERSION     DAWHSK010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15258
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.019"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(203..278)
                     /locus_tag="WIC03_000432"
     tRNA            complement(203..278)
                     /locus_tag="WIC03_000432"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(243..245),aa:Asn,seq:gtt)
     gene            complement(371..730)
                     /locus_tag="WIC03_000433"
     CDS             complement(371..730)
                     /locus_tag="WIC03_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEZ3882985.1"
                     /translation="MQQLLISILEDLNNTSTDIIASAVISTDGLPMATMLPSHLNSDR
                     VGAISATLLALGSRSVQELACGELEQVMIKGKSGYILLSQAGKDAVLVLVAKETGRLG
                     LILLDAKRAARHIAEAI"
     gene            complement(753..1397)
                     /locus_tag="WIC03_000434"
     CDS             complement(753..1397)
                     /locus_tag="WIC03_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HEZ3882986.1"
                     /translation="MESTLSLQANLYPRLTPAGAFYAVSSDAPSAGKTLLHSLLKADV
                     DEMVSSEKLLTWADTADIDTALNLLYRLQKLEFLYGDENGHSDGINLSDEQLPLLMEQ
                     LSGSGKALLVDRNGLYLANANFHHEAAEELGLLAAEVAQMEKKYRLLIKNNLYINNNA
                     WGVCDPSGQSELTFFPLYIGSTKFILVIGGIPDLGKEAFVTLVRILYRRYSNRV"
     gene            complement(1399..1935)
                     /locus_tag="WIC03_000435"
     CDS             complement(1399..1935)
                     /locus_tag="WIC03_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HEZ3882987.1"
                     /translation="MRENKIIFTGPVGVGKTTAIAAISDEALVQTDASASDMTLDRKR
                     NTTVAMDYGAISLDEDTKVHLYGTPGQERFNFMWEILSQGSMGLVLLLDNARTNPLKD
                     LEFFLHSFRGLLEKAPVVVGITKMDIRSQPGIDVYHKYLAKHNLNVPVFEIDARKEDD
                     VKQLVSAMLFSIDPGLEV"
     gene            complement(1947..3044)
                     /locus_tag="WIC03_000436"
     CDS             complement(1947..3044)
                     /locus_tag="WIC03_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEZ3882988.1"
                     /translation="MEVQLPKIKTVRVMLAGMTAQQESVFKMAFKMHNTTRYETVSPS
                     DGSAVPDLVLADTDAEGGFELWKELAERYKDIPVAVCSEKVPDSEVPYLPKPIRFETL
                     FPMLRKLLQGENVYGKSFIAPADRSAKNNGNVQRTVTIRQFNPNKGLLGALRFAEKNR
                     QDIAILHGNKPVLIVFPSIQRVLLTESVQKLEELCKDENLQVSCKTVPDNPQWREKAK
                     VGIMSCMWQFSIWTAQGRLIYPISPDTPFTLKSWPNLTRLANVPGSIRLSAFLTKASV
                     NLNVLYKVMPLNLNDILNYLAATYTTGFLSVDLKTVSQQAYSDMADKINIGADSASDS
                     EMMKKAEKITTPSQSQSRGLLQRLMKKLLGS"
     gene            complement(3047..4483)
                     /locus_tag="WIC03_000437"
     CDS             complement(3047..4483)
                     /locus_tag="WIC03_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEZ3882989.1"
                     /translation="MENSLIIGLALAVLLMLLVMRAKQGKKTPKRKSQGVGNTQGQTP
                     GSNDSDWVDQIGQSVSDGTQPDWSWNESAETASAAVSAQEVDPLTEYQVYKQFGYQGK
                     AAESLAAYLDGIPDGEAKPENLIRELLDINLEVGDVDVLADNLQKYGKLIPSELLAKY
                     IEQALQRDSNHLRIRVLAEEGLGWGTQEIEKRAEGGSATAASASPPPDAGGKAYEAEE
                     IKRIPIVRGKKDVSGISQEEIGAIAGLVRADQGAKILKDKVSYETASKQYDRAIQTSE
                     KPANLIIDALKLDYQHADIDRFAGHLWKLYQTLGNYGRQVKERMLGWGYSLGYHEVFD
                     DLEKGPNDRQIKDIGMGHGYLPKNIQKFKSQHRDLVLQDSSLINTGSSPADDAVKEVE
                     SLLMYGQIEAAMDVLEQAVLKYPDESQLYITLIDIYERTEDWDRLGQFLRVLRERADR
                     LPEEVVMLMSRLLQRMNQNIKKIKRYGK"
     gene            complement(4703..5485)
                     /gene="bioC"
                     /locus_tag="WIC03_000438"
     CDS             complement(4703..5485)
                     /gene="bioC"
                     /locus_tag="WIC03_000438"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HEZ3882990.1"
                     /translation="MESLTAINKSRIRQAFQKALNDYDRHALIQQKMTINLMTHLQDY
                     LPDMPLENVLELGCGSGMLSALLQKQISANYWLFNDLCDVQPQLAEKLPQSFDFYCGD
                     AENFPFQRQFDLIASASAVQWFHQPDAFIAHCKTGLKANGLLAVATFGKDNLKEVRQI
                     TNIGLNYPTLSQWQTWLAKDFELLWCEDFKVILDFDTPLDVLKHLKYTGVTATNQKNW
                     TRKNLNEFIGDYLSAFSMPSGKVHLTYHPLFFIARYSAAGRQ"
     gene            complement(5473..6120)
                     /locus_tag="WIC03_000439"
     CDS             complement(5473..6120)
                     /locus_tag="WIC03_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF452 family protein"
                     /protein_id="HEZ3882991.1"
                     /translation="MKTKFYNHQGGHLILYFAGWGTPPDAVNHLILPENHDLLICYDY
                     QDLNLDFDFSAYRHIRLVAWSMGVWAAERALQGIRLKSATAVNGTGLPCDDNFGIPCT
                     VFKGTLENLTENTRLKFERRMCGDKASFEDYQQFPARPFGEIHQELIALFAMIGQDRR
                     TDLIRWTNALVGSGDKIFMPANQHRYWTPRCTVREIDVGHYLFSRFTHWSALWNH"
     gene            complement(6130..7272)
                     /gene="bioF"
                     /locus_tag="WIC03_000440"
     CDS             complement(6130..7272)
                     /gene="bioF"
                     /locus_tag="WIC03_000440"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HEZ3882992.1"
                     /translation="MKVFKQQLEQLGAQNQYRSIPDLIHQGRYITRENCKMLNMSSND
                     YLGLASDENLRRSFLQQYGGNFPSFTSSSSRLLTGNFPIYTDLEELVAQRFQRESALL
                     FNSGYHANLGILPALTTTKSLILADKFVHASMIDGIRLSRCAFSRYRHNDYEHLKNLL
                     EKNVGKFDRTFIVTESVFSMDGDVADLKQLVQLKKQFPNTYLYVDEAHAIGVYGQNGL
                     GIAERDNLIAEIDLLVGTFGKALASVGAYAVCNQVLKECLINQMRPLIFSTALPPFNV
                     AWTYFIFERLPQFSKERSHLEQLSAFLRREVAHRTQIMPSQTCIVPYILGGNEAALAK
                     AEYLQRQGYYCLPIRPPTVPKNTSRIRLSLTADMTTDEVRQFAVCL"
     gene            7495..8571
                     /locus_tag="WIC03_000441"
     CDS             7495..8571
                     /locus_tag="WIC03_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEZ3882993.1"
                     /translation="MICRKPIGHTPYFDMNKTICRTAALLISGFSYANTVIPDVSPVA
                     QGQHVFINIPQQRLFLYTDGKLTKVYPVAVGRAMTQTNLGEHKIGAKAYNPVWYIPKS
                     IQKERGDGVKTIAAGPDNPLGPVFVRLGDPKLGLGIHGTNAPASVPGVRSHGCIRMKS
                     PDALEFAKTIASGSPASVIYQMAGLNEDADRNLWLAAFRDPYGKNNLDIASLKKSIAQ
                     WAKTQGKTIAPEKVDAVLKDRTGSAVCLTCGKNGKMKMPLKSLAWIQGSSSYSQAEVI
                     EQTEETNSAEISETRTPEVPDVHTPEAQPHLNTQSDGTPTAYTEPAADSSPQVETPDQ
                     AASEPVDVLFSIDVIRQGNLRLGN"
     gene            complement(8778..10244)
                     /locus_tag="WIC03_000442"
     CDS             complement(8778..10244)
                     /locus_tag="WIC03_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HEZ3882994.1"
                     /translation="MMKLGFKPIPLAIAAVLCALVLALPVPDGVKPQAWTLLAMFVGV
                     IAAIIGKAMPLGALSIIAVGLVAVTGVTADKPGAAMSDALSAFANPLIWLIAIAVMIS
                     RGLLKTGLGMRIGYLFIAVFGRKTLGIGYSLALSELLLAPVTPSNTARGGGIIHPIMQ
                     SIAGSYGSNPAKGTEGKMGKYLALVNYHSNPISSAMFITATAPNPLIVNLIAENLGSS
                     FRLSWGAWAWAMAVPGVIAFFVMPLILYFLYPPEIKETPNAVQFAKDRLREMGKMSAD
                     EIIMAVIFGILLLLWADVPALITGNHAFSINATATAFIGLSLLLLSGVLTWDDVLKEK
                     SAWDTIIWFGALIMMAAFLNKLGLIKWFSGVLAESVGGLGVSGTAAGVILVLAYMYAH
                     YMFASTTAHITAMFGAFLAAAVSLNAPAMPTALMMAAASNIMMTLTHYATGTSPVIFG
                     SGYTTMGEWWKAGFIMSVVNFLIFFVIGSIWWKVLGYW"
     gene            complement(10442..11365)
                     /gene="speB"
                     /locus_tag="WIC03_000443"
     CDS             complement(10442..11365)
                     /gene="speB"
                     /locus_tag="WIC03_000443"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEZ3882995.1"
                     /translation="MQYSTLAGQTDNSLVSNNFGFLRLPLNFMPYESQADWVITGVPY
                     DMAVSGRSGARFGPEAIRRASVNLAWEHRRFPWTFDVRERLNIIDCGDLVFSFGDSRD
                     FVEKMEAHAGKLLSSGKRCLSLGGDHFITLPLLRAHARYFGKLALIHFDAHTDTYDNG
                     SEYDHGTMFYTAPKEGLIDPSRSVQIGIRTEHSKKLPFTVLTAPQVNEDSVEETVRKI
                     KETVGNMPVYLTFDIDCLDPSFAPGTGTPVCGGLSSDRALKILRGLTDLDIVGMDVVE
                     VAPSYDQSDITALAGATIALEMLYLQGAKKD"
     gene            complement(11464..13356)
                     /gene="speA"
                     /locus_tag="WIC03_000444"
     CDS             complement(11464..13356)
                     /gene="speA"
                     /locus_tag="WIC03_000444"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HEZ3882996.1"
                     /translation="MPILTIREVCNINHWGIGYYDVDDSGEIIVRPNPSQHNQTVSLQ
                     KLTEAVQQKHQARLPVLFCFPQILEHRLRDINRAFQTAREECGYKGGYCLVYPIKVNQ
                     HRRVIESLMSSGQPHGLEAGSKAELMAVLAHAGTRQTLIVCNGYKDREYIRFALMGEK
                     LGHQVYLVIEKLSEIQMVLEEAEKLGIKPRLGVRARLASQGSGKWQSSGGEKSKFGLS
                     ASQVLQLVDILKQKNRLDCLQLLHFHLGSQLGNIRDVATGVHESARFYVELHKLGVNI
                     RCFDVGGGLGVDYEGNRTQSDCSVNYSLNEYAATVVWGISQACLEHGLPHPTIITESG
                     RGITAHHAVLVANVIGVERYKPRRLDAPSPEAPRVLHSMWETWTDISASREKRSLRSW
                     IHEGQFDLADVHNQYNVGLLSLAQRAWAEQLYLNICHEVGELFNEKHRSHRTIIDELQ
                     ERFADKLYVNFSLFQSLPDAWGIDQLFPVCPITGLNEPIARRAVLLDITCDSDGTIDH
                     YIDGDGIAGTMPMPDYPEEEPPLLGFFMVGAYQEILGNMHNLFGDTATADVVVGEDGQ
                     FTVIDYDEGNTVADMLEYVYQDPKELMKRYREQIEHSDLPASQAMSFLKELEAGLNGY
                     TYLEDE"
     gene            complement(13523..13807)
                     /locus_tag="WIC03_000445"
     CDS             complement(13523..13807)
                     /locus_tag="WIC03_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEZ3882997.1"
                     /translation="MKKLLLVAVVSLSAAAAFAGDSAERQIYGDPHFEQNRTKAVKML
                     EQRGYQVHDVDADDHWGKPVLEVEAYKDGREYDIVLSYPDLKIIKEQLDR"
     gene            complement(13958..>15258)
                     /gene="aspS"
                     /locus_tag="WIC03_000446"
     CDS             complement(13958..>15258)
                     /gene="aspS"
                     /locus_tag="WIC03_000446"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEZ3882998.1"
                     /translation="PEGARDYLVPSRVHPGEFFALPQSPQLFKQLLMVAGFDRYYQIT
                     KCFRDEDLRADRQPEFTQIDLETSFLNEDEIMDITEGMAKQVFKDALGVDLGDFPRMP
                     YSEAMFYYGSDKPDMRINLKFTELTDLMKTEEFKVFRGAADMKGGRVVALRVPNGTKF
                     SRKEIDEYTKFVGIYGAKGLAYIKVNDVGNLSNGEDSGLQSPIVKFLSENALKEIIAR
                     TGAQNGDIIFFGADKTKVVNEAIGALRIKVGLEHGKDNGYFTDEWKPLWVVDFPMFEY
                     DEEADRYVAVHHPFTAPKEGHEDLMVSDPANCLARAYDMVLNGWEIGGGSIRIHRADV
                     QEKVFAALKISPEEQQEKFGFLLDNLKFGAPPHGGLAFGLDRLVTLMTGAESIRDVIA
                     FPKTQRAQCLLTNAPNSVDDKQLRELSLRLRQKAAETKEA"
BASE COUNT         3991 a         3877 c         3471 g         3919 t
ORIGIN      
        1 caatgccgta ctggtttttg ttaatccact ataaaatcga gtttatccta gctgtccagg
       61 acagccccta ttttttcata accatcacga aaggaatttt gcaaagattt tattccatct
      121 caaaacaatc atctcaaaaa tgcgtttctg accgccggta aaacaaaacc ctctaagaaa
      181 atacttagag ggttttgaaa tttggctccc cgacctgggc tcgaaccagg gacctgcgga
      241 ttaacagtcc gtcactctac cgactgagct atcggggaat aagcgcgaat aataaaacag
      301 aaataccgaa cgtgtcaata taaattgaca atcgattgca ctttatctgc aagcccgcaa
      361 tctttatatg ttatatggct tccgcaatat gccttgccgc acgtttggca tccaatagga
      421 ttaaaccaag tctgccggtt tcttttgcca ccagcaccaa cacggcatct ttacccgcct
      481 gacttaaaag gatatagcct gattttcctt taatcatcac ttgttccaat tccccgcagg
      541 cgagttcctg caccgagcga ctccccaaag caagcaaagt ggcagaaatc gcccctaccc
      601 tgtccgaatt caaatgtgaa ggaagcattg tcgccatcgg caatccgtcg gttgagataa
      661 cggcagacgc gataatatcc gtagatgtat tgtttaaatc ttcaaggatt gaaatcaata
      721 attgctgcat aacccctcct ctctcccaag ttttacacgc ggttgctgta acggcggtat
      781 aaaatcctta ccaaagtaac aaatgcctct ttgcccaaat cgggaatgcc gccgataacc
      841 aaaataaatt tggttgaacc gatatacaat gggaaaaatg tcaattcgct ctgaccggaa
      901 ggatcgcaaa cgccccaagc gttattgttg atatacaggt tgttcttaat cagcagccgg
      961 tatttctttt ccatctgtgc gacttctgcc gccaacaacc ccaactcttc cgccgcctca
     1021 tgatggaaat tggcgttggc aagatacaga ccgttccgat cgaccaataa cgccttaccg
     1081 ctgccggaca attgttccat cagcaacggc aattgctcgt ccgacaaatt gatgccgtct
     1141 gaatgaccgt tttcatcgcc atagaggaat tcgagttttt gcaaacggta caacaggttc
     1201 aaagcggtat cgatgtcggc ggtgtccgcc caggtaagca gcttctcact gctgaccatt
     1261 tcgtccacat ccgctttcaa caggctgtgc aacaaagttt taccggcact gggggcatcg
     1321 ctggatacgg cataaaatgc accggcagga gtcaggcggg gatataaatt tgcttgtagt
     1381 gaaagtgttg attccatatt aaacctccag tcccggatca atagaaaata acattgcgct
     1441 aaccaattgt tttacgtcat cttccttacg ggcatcaatt tcaaaaaccg gaacattaag
     1501 attatgtttt gcaagatatt tgtgatacac gtcgataccg ggctgagagc gtatatccat
     1561 cttggtaata ccgacaacga cgggtgcctt ctccagcagc cctcgaaacg aatgtaaaaa
     1621 gaattccaaa tctttcaacg gattggttcg ggcattatct aaaagcaaga ccaaacccat
     1681 actgccttgg cttaagattt cccacataaa gttgaaccgt tcctgaccgg gcgtaccata
     1741 taaatggact ttggtatcct catccaagct gatggccccg tagtccatcg ccactgtcgt
     1801 attccttttc ctatccaaag tcatatcgga tgcggaagca tcggtctgaa cgagtgcttc
     1861 gtccgaaata gccgcaatgg cagtggtttt ccctacgccg acaggtcctg tgaaaataat
     1921 tttattttct ctcatctctc cgcctcttag ctgcccaaca gttttttcat cagcctttgc
     1981 agaagaccgc gcgactggga ttgggatggt gttgtgattt tttccgcttt tttcatcatt
     2041 tcactatcag aggcagaatc ggctccgata tttattttat ccgccatatc ggagtatgcc
     2101 tgttgtgaaa ccgtttttaa atctaccgac aaaaacccgg ttgtataggt tgccgcaaga
     2161 taattcagaa tatcattgag gtttaaaggc atcactttat acaacacgtt aaggttgacg
     2221 gatgccttgg tcagaaatgc cgacaagcgt atcgaccccg gcacatttgc caaccgggtc
     2281 aggtttggcc aagatttcaa cgtaaacgga gtatcgggag aaatcggata aatcaacctg
     2341 ccctgcgccg tccaaatgga aaactgccac atacaggaca taatgcctac tttagccttt
     2401 tcgcgccatt gcgggttatc gggaacagtc ttgcagctga cctgcaaatt ttcgtctttg
     2461 cacaattctt cgagtttttg cacactttct gtcagcaaaa cccgttgtat cgaggggaaa
     2521 acaataagga ccggcttatt tccatgcaag atagcgatgt cctgcctgtt cttttccgca
     2581 aaccgcaacg cccccaataa tcctttattc gggttaaact ggcgtatcgt aaccgtacgc
     2641 tgcacattcc cgttattttt cgccgaccgg tctgcgggtg caataaacga tttcccataa
     2701 acattctcgc cctgcaacaa tttgcggagc ataggaaaca atgtttcaaa ccgaatcggt
     2761 ttgggcaggt agggaacttc agaatcggga actttctccg aacagacggc gacgggtata
     2821 tccttataac gctcggcaag ctctttccaa agttcaaaac cgccctcggc atcggtatcc
     2881 gccaaaacca aatcgggcac ggcactgccg tctgaagggg atactgtttc ataacgggtg
     2941 gtattgtgca ttttgaatgc cattttgaaa acggattcct gctgcgccgt catccccgcc
     3001 aacattacgc gtactgtttt aattttcggc agttgaactt ccatttttat tttccgtacc
     3061 gttttatttt tttaatattt tgattcatac gctgcagcag ccggctcatc agcataacga
     3121 cctcttcagg aagcctgtcc gcacgttccc tcaataccct taaaaactgc cccaacctat
     3181 cccaatcttc agtacgttca taaatatcga tcaacgtaat ataaagctgg gactcgtcgg
     3241 gatatttcaa taccgcctgc tccaacacat ccattgccgc ttcaatctga ccatacatca
     3301 gcaacgactc tacttcctta accgcatcgt ctgccggaga cgaaccggtg ttgatcaacg
     3361 aagaatcttg aagcaccaaa tcccgatgtt gcgatttgaa tttctgtata tttttcggca
     3421 gatacccgtg ccccataccg atgtctttga tttgccggtc gttcggccct ttttccaaat
     3481 catcgaaaac ttcatggtaa cccaagctgt acccccaccc cagcatccgc tctttaacct
     3541 gcctgccgta gttgcccaac gtttggtaaa gtttccacaa atgtccggca aaacggtcta
     3601 tgtccgcgtg ttggtaatcg agtttcaacg catcgataat caggtttgca ggtttttcgg
     3661 aagtttggat ggcacggtcg tattgtttcg atgccgtttc atagctgact ttgtctttaa
     3721 ggattttcgc accttgatcg gcgcgaacca aaccggcaat cgcaccgatt tcctcttgac
     3781 tgattccgga cacgtctttt ttgccccgca caatcgggat gcgcttgatt tcttcggctt
     3841 cataagcctt accgccggca tccggcgggg gcgatgcgga agctgccgtc gcagaaccgc
     3901 cttccgcacg tttttcaatc tcctgagtgc cccatcccaa accttcttcc gccaagacac
     3961 ggatacgcaa atggtttgaa tcgcgctgta atgcctgttc gatatatttt gccaaaagtt
     4021 cggaaggaat cagtttgccg tatttttgca gattgtctgc caaaacatcg acatccccca
     4081 cttcgagatt gatatcgagc agctcgcgga taaggttttc aggtttcgct tcaccatccg
     4141 gaatgccgtc cagataggca gccaaagatt cggcagcctt gccctgataa ccgaattgct
     4201 tataaacctg atactccgta agcggatcga cttcttgcgc ggatacggcg gcggatgcgg
     4261 tctcggcact ttcgttccaa gaccagtcgg gttgcgtgcc gtctgaaacc gattgtccaa
     4321 tctgatcgac ccagtccgaa tcattgcttc cgggtgtctg cccctgcgta ttgccgacac
     4381 cttgggattt gcgcttcggt gttttcttac cctgcttcgc acgcataacc aaaagcatca
     4441 gcaataccgc cagtgccaaa ccgataatta atgagttttc caaaggatac tcccgatacc
     4501 gtaacgagcg gatagctgac cgcctgcaac ggccattgtc cgcttttatt attaacgatt
     4561 tactgaaata gacttgtaag ttttaaatca taccataatt taacgtttaa caatatgcct
     4621 tctgcacaag cctcgccata ttacctttta cccacatcag tatcaatacc cgatataaaa
     4681 ataactttgc ccataagctg ccttattgcc tgcccgccgc agagtagcgc gcgataaaaa
     4741 ataacggatg ataagtcagg tgcactttgc ccgacggcat actgaacgcc gacaagtaat
     4801 cgccaataaa ttcattgaga ttttttcttg tccaattttt ttggttcgtg gctgttacgc
     4861 ctgtatattt aaggtgtttg agtacatcta acggcgtatc aaagtctagt attaccttaa
     4921 aatcctcaca ccataaaagc tcaaaatctt tggctaacca agtctgccat tgggataaag
     4981 tcgggtaatt taagcctata tttgtaattt ggcggacttc ttttaaattg tctttgccaa
     5041 aggttgcaac cgccaataat ccgtttgctt tcaagcctgt tttgcaatgg gcgataaaag
     5101 cgtcgggttg atgaaaccat tgcacggcag atgcgcttgc gattaagtca aattgtcgtt
     5161 gaaaaggaaa gttttccgca tcgccgcaat aaaaatcaaa ggattgcggc agtttttcag
     5221 ccagttgggg ctgcacatcg cacaaatcat taaataacca ataattcgct gaaatctgtt
     5281 tttgcagcaa ggcactcaac attcctgagc cgcagcccaa ttccaacacg ttttccaatg
     5341 gcatatccgg caaataatct tgcaaatgcg tcattaaatt aatcgtcatt ttttgttgga
     5401 ttaaggcgtg ccggtcataa tcgtttaatg ctttttggaa agcctgccga atgcgcgatt
     5461 tatttatggc agtcagtgat tccatagtgc cgaccaatgg gtgaatcttg aaaacaggta
     5521 atgtccgacg tcaatttccc gaacggtgca acgcggtgtc caatatcggt gctgattggc
     5581 aggcataaaa attttatcgc ccgatccgac caaggcattt gtccagcgga taagatctgt
     5641 acgtctatct tgcccgatca tcgcaaaaag tgcgataagt tcttgatgaa tttcgccaaa
     5701 cgggcgtgcg ggaaattgtt ggtaatcttc aaaagatgct ttatcgccac acattctgcg
     5761 ttcaaatttt aaacgggtgt tttccgtgag gttctccaat gtgcctttaa aaacggtgca
     5821 agggataccg aaattatcat cgcaaggcaa acctgtgcca ttcactgccg ttgcggattt
     5881 taatcttatt ccttgcaatg ccctctctgc cgcccaaacg cccattgacc acgccaccaa
     5941 acggatgtgc cgataggcgg aaaaatcaaa atccaaattt aaatcttgat aatcatagca
     6001 aatcaataaa tcgtgatttt ccggcaaaat caaatgattt acagcatcgg gcggcgttcc
     6061 ccaacctgca aaatacagga ttaaatgtcc gccttgatga ttgtaaaatt ttgttttcat
     6121 atcatatcct tacaggcaca ccgcaaactg ccgcacttca tccgttgtca tatctgccgt
     6181 taaagacagg cggattctgg atgtgttttt gggtactgtc ggcggtctga tgggcaggca
     6241 ataataaccc tgcctttgca gatattccgc tttggcaagg gcggcttcat tcccgcctaa
     6301 aatatagggg acgatacagg tttggctcgg cattatttgc gtccgatgcg ccacttcccg
     6361 ccgtaaaaat gcacttaact gctcaagatg gcttctttct tttgagaatt gcggcaatcg
     6421 ttcaaaaata aaataagtcc aagccacatt aaacggcggc aatgcggttg aaaaaatcaa
     6481 tgggcgcatt tgattaatca aacattcttt caatacttgg ttgcagacgg catacgcccc
     6541 caccgaggct aaggctttac cgaaagtgcc gaccagtaaa tcaatctcgg caatcaaatt
     6601 atcccgttcg gcaatcccca atccgttttg cccataaaca ccgattgcgt gggcttcatc
     6661 cacataaaga taagtattgg gaaactgttt ttttaattgg acaagctgtt tcaaatccgc
     6721 cacatcgccg tccatactga aaacagattc ggtaacgata aaagtgcggt caaattttcc
     6781 gacgtttttt tcaagcagat ttttcaaatg ttcataatca ttatgacgat aacgggaaaa
     6841 cgcacaccgg ctcaaacgga tgccgtcaat catactggcg tgaacaaatt tatctgccaa
     6901 aatcaaactt ttcgtcgtcg tcaaagcagg caaaataccg agattggcgt gatagccgct
     6961 gttgaacaat aacgcgcttt cccgttggaa acgttgtgcg acaagctctt ccaaatcggt
     7021 ataaatagga aagttgcccg ttaataaacg cgatgaagaa ctggtaaaag agggaaaatt
     7081 accgccgtat tgctgcaaaa aagaccggcg caagttttca tctgatgcca aacccaaata
     7141 atcattagac gacatattca gcattttgca gttttcccgc gtaatatacc gcccttgatg
     7201 aatcaaatcc ggaatcgaac gatattggtt ttgcgcgccg agttgttcaa gctgttgttt
     7261 aaaaaccttc attatgatgt aaatattcct gagttaaagc ctgaaccaac ccagcatcaa
     7321 acaatcaagt aatgcccttt taataaaaac cgttttgccg ccgacctttg ccacacccct
     7381 gccttcataa tccaaggcgg aaatttccgc gacatttgtt tccgttgcca ttacccaatc
     7441 cgtccgcact gtaccgaagc gcatattttc gcacaaattg gtacggtttc aggtatgatt
     7501 tgccgaaaac ctatcggaca cacaccatat tttgatatga ataaaacaat ctgccgcact
     7561 gctgcactat tgataagcgg tttttcatac gcaaacaccg tcattcccga tgtctccccc
     7621 gtcgcacaag gtcaacatgt cttcattaac atccctcagc aacgcctgtt cctctacacc
     7681 gacggcaaac tgaccaaggt ttatcccgtt gcagtaggtc gggcgatgac gcaaaccaat
     7741 ctaggcgaac ataaaatcgg tgcgaaagcc tataaccctg tttggtacat ccccaaatcc
     7801 atacagaaag aacgcggcga cggcgtaaaa accatagccg ccggcccgga caaccctttg
     7861 gggccggttt ttgtccgctt aggcgacccc aaactcggtt tgggcataca cgggaccaat
     7921 gcgccggcca gcgtccccgg ggttcggagt cacggctgca tccgcatgaa atcgcccgac
     7981 gcgctcgagt ttgccaaaac catcgccagc ggctcgcccg cctccgtcat ctatcaaatg
     8041 gcggggctca atgaagatgc ggatcgcaac ctgtggcttg ccgccttccg cgacccttac
     8101 ggtaagaaca accttgacat cgcctctctg aaaaaaagta ttgcgcaatg ggcaaaaaca
     8161 cagggtaaaa ccatcgcgcc cgagaaagtc gatgctgtac tcaaagaccg caccggatcg
     8221 gccgtctgcc tgacctgcgg caaaaacggc aagatgaaga tgccgctcaa atcgctggcg
     8281 tggatacagg gttcttcctc atacagccaa gctgaagtca ttgaacaaac tgaggaaacc
     8341 aactctgccg aaatatccga gacacgcacg cccgaagtgc ctgatgtaca cacgcccgag
     8401 gcgcaaccgc atttaaatac ccaatccgac ggcacgccga ctgcctatac cgaaccggct
     8461 gccgattcat cgccgcaagt agaaacacct gatcaggctg cttccgagcc ggttgatgta
     8521 ttattttcaa tagatgtgat acggcaggga aatttgcgtt taggtaattg aataaccttc
     8581 tgattattta atgcttatgt ttatcagaag ttgataggcg gtttggtttt tgtttgactt
     8641 ttcctacacc gccgcctgcc gctttctgca acattcaagc gcacaaatat gccatctgaa
     8701 ggctttagac ggcatatttc acgataatag tcgcccaatc aaataaacac agattggaaa
     8761 tttattttat ttttccctta ccaatacccc agaactttcc accaaatgct gccgataacg
     8821 aaaaaaatca gaaaattgac tacgctcatg ataaaacccg ccttccacca ttctcccatt
     8881 gtggtgtagc ccgaaccgaa aatcacaggc gaagtaccgg tcgcataatg agtgagggtc
     8941 atcataatgt tggatgcggc cgccatcatc agcgcggtcg gcatcgccgg tgcattcagt
     9001 gaaacggcag cagcgagaaa tgcgccgaac atagcggtaa tatgtgcagt agtactggca
     9061 aacatataat gcgcatacat ataagcaagc acgaggatta cgcccgcagc cgtgccgcta
     9121 acgcccaaac cgccgacact ttccgccaac actccggaga accatttaat cagtccgagt
     9181 ttatttaaaa atgcggccat cataatcaat gcgccaaacc aaataatcgt atcccacgca
     9241 cttttttctt tcaaaacatc gtcccaagtc aatacaccgg aaagcaaaag caggcttaat
     9301 ccgataaatg cggtggcggt ggcgttgata ctaaaagcgt gattgccggt aataagggcg
     9361 ggaacatctg cccacaacag cagcaagata ccgaaaatga ccgccataat gatttcgtct
     9421 gccgacattt tacccatctc cctcagacgg tctttggcaa attgaacggc attgggcgtt
     9481 tctttaattt caggcggata caaaaaatat aaaatcaaag gcataacgaa aaaggcgata
     9541 acgccgggaa cagccattgc ccacgcccac gccccccaag aaagacggaa actactgcct
     9601 aaattttcgg caatcaagtt gacgattaaa gggttggggg cagttgcagt aataaacata
     9661 gccgacgaaa tgggattgga atgatagttg accaaagcca aatatttacc catcttgcct
     9721 tctgtgcctt ttgcgggatt ggagccgtaa ctgccggcaa tcgactgcat aatcggatgt
     9781 ataatgccgc cgccgcgcgc ggtattggaa ggggtaacgg gagccagcag cagttcggaa
     9841 agagcgagac tgtaaccgat gcccagcgtt tttcttccaa aaacggcgat aaacaaatat
     9901 ccgatacgca tccccagccc tgttttgagc aaaccgcgcg aaatcataac tgcgatggca
     9961 atcagccaaa tcaacggatt ggcgaacgca ctcaacgcat cgctcatcgc cgcgcccggt
    10021 ttgtcggcgg ttacgccggt tactgcgacc aacccgacgg caataatcga cagcgcgccc
    10081 aacggcatgg ccttgccgat aatggcggca atcacaccga caaacatggc cagcagcgtc
    10141 caagcctgag gcttgacccc gtcgggtacg ggcagtgcca aaaccagggc gcacaatact
    10201 gcggcaatgg cgaggggtat cggtttgaaa cccaatttca tcatattgac ctccgtaaaa
    10261 aagaccgtcc cgaaaaatcg gaaaaataat atttaactaa ttgttttata agatatattt
    10321 agatatttca ccgtctttcc gatatgcggc tccgggcaac ttttgtttca gtatttgaat
    10381 tttcattaga ctgaatacgc cgtttgaacg gcacggcgaa aacccggggg atgccggacg
    10441 ttcagtcctt tttcgcacct tgaaggtaaa gcatttccaa ggcaattgtg gcgccggcca
    10501 aagcggtaat gtcggattgg tcgtaagagg gggcaacttc tacaacatcc ataccgacga
    10561 tgtcgagatc cgtcagccca cgtaggattt ttaatgccct gtcgctgctc aagccgccgc
    10621 atacgggcgt accggtcccg ggggcgaacg acgggtcgag gcagtctatg tcgaaagtca
    10681 ggtaaacggg catattgccg acggtttctt tgattttacg gacggtctct tcaacactgt
    10741 cttcattgac ttggggggcg gtcaacacag taaaaggcaa ttttttactg tgttcggtgc
    10801 gtatgccgat ttgtacggaa cgggacgggt cgatgaggcc ttccttgggg gcggtataga
    10861 acatcgtacc gtggtcgtat tcgctgccgt tgtcgtaggt gtcggtgtgc gcgtcaaaat
    10921 gaatcagtgc gagtttgccg aaatagcggg cgtgggcgcg caacaacggt agggtaatga
    10981 aatggtcgcc gcccaaactc aaacagcgtt tgccggaaga aagtaatttg ccggcgtgcg
    11041 cttccatttt ttcgacaaaa tccctgctgt cgccaaaaga aaaaaccaag tcgccgcaat
    11101 caataatgtt caggcgttcg cgcacatcaa atgtccatgg aaacctgcgg tgctcccaag
    11161 cgaggttgac ggaggcgcgc cggatggctt caggaccgaa acgcgcgccg gaacgccctg
    11221 aaaccgccat atcataaggc acgccggtaa tgacccaatc tgcttggctt tcatacggca
    11281 taaaattaag cggcaggcgc aaaaacccga aattattgga aacgagggag ttgtcggttt
    11341 gtcctgccag tgtgctgtat tgcatcgtaa tgattccttg taattggttt caatcggtcg
    11401 tggtgattgg tgtttgagga agaaaatcgg gcttcagacg gcatatccga tgccttgatg
    11461 cgtctattcg tcttccaaat aggtataacc attaagcccc gcttcgagtt cttttaagaa
    11521 agacatagcc tgcgaggcag gaaggtctga atgttcgatt tgttcgcgat agcgtttcat
    11581 cagctctttc ggatcttgat aaacgtattc gagcatatcg gcaacggtgt ttccttcatc
    11641 gtaatcgatg acggtaaatt gtccgtcttc ccctacaaca acatcagcag tggcagtgtc
    11701 gccgaaaaga ttgtgcatat tgccgagtat ttcctgatat gctcccacca taaaaaagcc
    11761 taaaagcggc ggctcttctt cgggataatc aggcataggc atcgtaccgg cgatgccgtc
    11821 gccgtcgatg tagtggtcaa tcgtaccgtc tgaatcgcag gtaatgtcca acaacacggc
    11881 gcggcgcgca atcggttcat tcaaaccggt aatgggacaa acagggaaaa gttgatctat
    11941 gccccaagca tcgggcaaag attggaagag tgagaaattg acatacagct tatcggcaaa
    12001 acgttcttgc aattcgtcaa taatggttcg gtgagaccgg tgtttttcat taaacaattc
    12061 gccgacttca tgacagatat ttaaatacag ttgctccgcc cacgcacgtt gcgccaaact
    12121 caacagcccg acattatact gattatgcac atcggcaaga tcaaactgcc cttcgtgtat
    12181 ccagctgcgt aaggaacgtt tttcccgcga ggcggaaata tccgtccaag tttcccacat
    12241 actgtgcaac acacgcggcg cttcgggcga tggcgcatcc agccggcgcg gtttgtaacg
    12301 ttcaacgcct ataacattag caaccaaaac ggcgtgatgt gcggtaatgc cgcgcccgct
    12361 ctcggtaatg attgtcggat gcggcagccc gtgttcgaga caagcctggc tgatgcccca
    12421 tacgactgtg gcggcatatt cgttgaggct gtaattaacg gaacaatccg attgtgtgcg
    12481 gtttccttcg taatccacgc caagcccgcc gcctacatca aaacagcgga tatttacccc
    12541 cagtttgtgc aactcaacat aaaaccgagc cgattcgtgt acacctgtgg caacatcacg
    12601 gatgttccca agctgcgagc ctaaatggaa atgcaaaagc tgcaggcagt ccagcctgtt
    12661 tttttgtttc aaaatatcga ccagttgcaa aacttgggaa gccgacaagc cgaattttga
    12721 tttttcccca cccgaagact gccattttcc cgaaccttgg gaagccagtc tggcgcgcac
    12781 acccaaacgg ggcttgatgc cgagtttttc cgcctcttcc aataccattt gtatttcgga
    12841 cagcttctca atcaccaaat aaacctgatg ccccagtttt tcgcccatca aggcgaaacg
    12901 gatatattca cggtctttat agccgttgca gacgattaat gtttgccggg tgccggcgtg
    12961 tgccaaaacc gccatcagtt cggctttaga accagcttcc aaaccatgcg gttgtccgct
    13021 tgacataagc gattcgatga cgcggcggtg ttggttgacc ttgatagggt aaaccaaaca
    13081 ataaccgccc ttatagccgc actcttcccg tgccgtctga aaggcgcggt taatgtcgcg
    13141 gaggcggtgt tcgaggattt gcggaaaaca aaacaaaaca ggcaggcgcg cctgatgttt
    13201 ttgttgcacg gcttcagtca gtttttgcag tgaaacagtt tgattgtgtt gcgagggatt
    13261 ggggcggacg atgatttcgc cggaatcgtc aacatcataa taacctatgc cccaatgatt
    13321 aatgttgcac acttcacgga tggtaaggat aggcataata aacctgctcc gtctgtcgtg
    13381 ttgaaaagga atgattataa caaatcagcg tgaaatgtca tttttttaat aagaaaagcc
    13441 tgcctcatac ctgatgagaa acaggcaaaa tgccgtctga acgcttcaga cggcattttg
    13501 gttcagcttt ccatcaaagg aatcagcgat cgagctgctc tttgatgatt ttcaggtcgg
    13561 ggtaagacaa cacgatgtcg tattcgcggc cgtctttata ggcttccact tccaaaacag
    13621 gtttgcccca atggtcgtcg gcatcgacat cgtgaacctg ataaccgcgc tgttccaaca
    13681 ttttcacggc ttttgtgcgg ttttgttcaa aatggggatc gccgtaaatc tgacgctcgg
    13741 cagagtcgcc ggcaaatgcg gctgcggcac tcagggaaac aacggcgacc aataacagtt
    13801 ttttcatttt cagtcctttt tttatcggtt gattgaacaa gatgtgtttt tcaataccgc
    13861 cattaaaaca cagcaaaatt aggtttgaat tagagggaag tcaagaagcg taaatgccgt
    13921 ctgaaaaaaa cagagccgtc aaacggctct gttttcctta tgcttctttg gtttcagccg
    13981 ccttttgacg caggcgcaag ctcaattcgc gcaactgctt gtcgtccacg ctgttgggcg
    14041 cgttggtcag caggcattgg gcgcgttgtg ttttcgggaa ggcaatcacg tcgcggatag
    14101 attcggcgcc ggtcatcagg gtaaccaaac ggtcgaggcc gaatgcaagg ccgccgtgag
    14161 gaggcgcacc gaatttcagg ttgtccaaga ggaagccgaa tttctcttgt tgctcttcag
    14221 ggctgatttt cagcgcggca aacactttct cttgtacgtc tgcacggtgg atacggatag
    14281 agccgccgcc gatttcccag ccgttcaata ccatgtcgta ggcacgggcc aggcagtttg
    14341 cagggtcgga aaccatcagg tcttcatgac cttctttagg cgcggtaaac ggatggtgta
    14401 cggcaacgta gcggtcggct tcttcgtcgt attcgaacat tgggaaatcg acaacccaca
    14461 aaggtttcca ttcgtctgtg aagtagccgt tatctttgcc gtgttccaag ccgactttga
    14521 tacgcaatgc gccgatggct tcgttcacaa ctttggtttt gtctgcaccg aagaagataa
    14581 tatcgccgtt ttgtgcgccg gtacgcgcga tgatttcctt cagggcattc tcagacagga
    14641 attttacgat tggagattgc aggccgctgt cttcgccgtt ggagaggttg cccacatcat
    14701 ttactttgat gtatgccaga cctttcgcac cgtagatgcc gacaaattta gtgtattcgt
    14761 cgatttcttt gcggctgaat ttcgtaccgt tcggcacacg cagggcaacc acgcggccgc
    14821 ctttcatgtc ggctgcgcca cggaagactt tgaattcttc cgttttcatc aggtcggtca
    14881 actcggtgaa tttcaagttg atacgcatgt ccggtttgtc agagccgtag tagaacatgg
    14941 cttcagagta aggcatgcgt gggaagtcgc ccaagtccac gcccagcgcg tctttgaaga
    15001 cttgtttcgc cataccttcg gtgatgtcca tgatttcatc ctcgtttaag aacgaggttt
    15061 ccaagtcgat ttgcgtgaat tcaggctgac ggtcggcacg caagtcttcg tcgcggaagc
    15121 acttggtgat ttggtagtaa cggtcgaaac ccgccaccat caacagttgt ttgaacaatt
    15181 gcggcgattg cggcagcgcg aagaactcgc ccggatgaac gcggctcggc acgaggtagt
    15241 cgcgcgcgcc ttcgggcg
//
LOCUS       DAWHSK010000020        15137 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.020, whole genome shotgun sequence.
ACCESSION   DAWHSK010000020 DAWHSK010000000
VERSION     DAWHSK010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15137
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.020"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(65..904)
                     /locus_tag="WIC03_000447"
     CDS             complement(65..904)
                     /locus_tag="WIC03_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
                     /protein_id="HEZ3882999.1"
                     /translation="MKQLRDNKAQGSALFTLVSGIVIVIAVLYFLIKLAGSGSFGDVD
                     ATTEAATQTRIQPVGQLTMGDGIPVGERQGEQIFGKICIQCHAADSNVPNAPKLEHNG
                     DWAPRIAQGFDTLFQHALNGFNAMPAKGGAADLTDQELKRAITYMANKSGGSFPNPDE
                     AAPADNAASGTASAPADSAAPAEAKAEDKGAAAPAVGVDGKKVFEATCQVCHGGSIPG
                     IPGIGKKDDWAPRIKKGKETLHKHALEGFNAMPAKGGNAGLSDDEVKAAVDYMANQSG
                     AKF"
     gene            1189..1264
                     /locus_tag="WIC03_000448"
     tRNA            1189..1264
                     /locus_tag="WIC03_000448"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Arg,seq:ccg)
     gene            complement(1329..2522)
                     /locus_tag="WIC03_000449"
     CDS             complement(1329..2522)
                     /locus_tag="WIC03_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HEZ3883000.1"
                     /translation="MYRHIEYYPGDPILSLVETFKNDPRPEKVNLSIGIYFDDEGKMP
                     VLESVSRAETARAATPVPSPYLPMEGLDTYRSAVQHLLFGKDNPALAQGRIVTVQTLG
                     GSGALKVGADFLHRWFPEARAYVSDPTWDNHRGIFESAGFEVGTYPYYDPAAVGVKFD
                     EMTAFFNTLPENSVLILHPCCHNPTGVDMSEQQWDEVLHIIKTRKLIPFMDIAYQGFG
                     GDLDSDAYAVRKAVEMELPLFVSNSFSKNLSLYGERVGGLSVVCPNKEEADLVFGQLK
                     FTVRRIYSSPPAHGAYIAADVMNSPELYALWQNEVYMMRDRIRAMRQKLYDVLTAQIP
                     DRDFTYFIKQRGMFGYTGLSVEQVRRLRDEFAVYLLDSGRMCVAGLNTSNTDYVARAF
                     AEVLK"
     gene            complement(2528..3631)
                     /gene="trmA"
                     /locus_tag="WIC03_000450"
     CDS             complement(2528..3631)
                     /gene="trmA"
                     /locus_tag="WIC03_000450"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HEZ3883001.1"
                     /translation="MSDYTQQLQDKKDHLKTLFAGLDVPEWEVYESPDKHYRMRAEFR
                     IWHEGGEMFYAMFEKGQKAGGASLIRCDRFEAASEAVNRLMPELIAVAAQSAELRNRW
                     YAVEFLSTLSGEMLVTMIYHKRLDNEWMQAAQALQQQLDISVIGRSRGQKIVLKQDYV
                     TETLKVGNRDFRYRQIEGSFTQPNAAVCQKMLEWACGAAEGLDGDLLELYCGNGNFTL
                     PLSEKFERVLATEISKTSVSAAQWNIEANRIGNIKIARLSAEEFTEAYTGKREFKRLK
                     DGGIALTDYAFSTIFVDPPRAGIDEETLKLVSQFDNIIYISCNPETLRTNLDTLAETH
                     TVERAALFDQFPFTHHIESGVLLKKKILGKSKR"
     gene            3774..4874
                     /gene="aroC"
                     /locus_tag="WIC03_000451"
     CDS             3774..4874
                     /gene="aroC"
                     /locus_tag="WIC03_000451"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEZ3883002.1"
                     /translation="MAGNTFGQLFTVTTFGESHGAGLGCIIDGCPPGLELSEADIQFD
                     LDRRKPGTSRHVTQRRETDQVEILSGVFKGKTTGTPIALLIRNTDQRSKDYGNIAQSF
                     RPGHADYTYWHKYGTRDYRGGGRSSARETAARVAAGAVAKKWLKEKFGTEITAYVTQV
                     GEKEIRFEGCEHISQNPFFAANHSQIAELENYMDSVRKSLDSVGAKLHIEAANVPVGL
                     GEPVFDRLDAEIAYAMMGINAVKGVEIGAGFDSVTQRGSEHGDELTPQGFLSNHSGGI
                     LGGISTGQDICVNIAIKPTSSIATPRRSIDINGNPIELATHGRHDPCVGLRAAPIAEA
                     MLALVLIDHALRHRAQNADVAVDTPDIARSDK"
     gene            4965..5369
                     /locus_tag="WIC03_000452"
     CDS             4965..5369
                     /locus_tag="WIC03_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant transport activator ProQ"
                     /protein_id="HEZ3883003.1"
                     /translation="MTQETALGAALKSAVQTMSKKKQTEMIADHIYGKYDVFKRFKPL
                     ALGIDQDLIAALPQYDAALIARVLANHCRRPRYLKALARGGKRFDLNNRFKGEVTPEE
                     QAIAQNHPFVQQALQQQSAQAAAEAAGSSAAE"
     gene            complement(5443..7428)
                     /gene="parE"
                     /locus_tag="WIC03_000453"
     CDS             complement(5443..7428)
                     /gene="parE"
                     /locus_tag="WIC03_000453"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEZ3883004.1"
                     /translation="MAKNNQYSESSITVLKGLEPVKERPGMYTRTDSPTHICQEVIDN
                     AADEALGGFATAIDVQIHEDGSLSVRDNGRGIPVGLHPEEGVPVVELVFTRLHAGGKF
                     NKKDGGSAYAFSGGLHGVGVSVTNALSTRLEVTVKREGKIHRIVFAGGDVVEPLAQVG
                     KCAVKDSGTEVRVWPDGKYFESPNYSIPELERLLRAKAVLLPGVRVSLTRPVKGEDEA
                     HTQTWHYPDGLKSYLTDLIADAQEAVPLFSCENYISDGHNGDFSIGEGAAFALTWLEE
                     GSCANESYVNLIPTPLGGTHEAGLKQAVFNAVNNFINLHNLLPRGVKVQSDDVFGKTA
                     FVLSARVLDPQFQGQTKDKLTNRDALKLVAAVSGDPLELWLNQNVDFGKKIAELAIRQ
                     AQARMRSVKKIEKKKGSGVAVLPGKLTDCESEDIRENELFLVEGDSAGGSAKLARDKA
                     TQAILPLRGKVLNSFEVHPDQLFGNAEIHDISVAIGVDPHGINDHPDLSGLRYGKIAI
                     LSDADVDGSHIQVLLLTLFYRHFPKLVADGHIYVAQPPLFRVDVNAQGKNKPARKFYA
                     LDQNELDSILERLQKEGFKETAYSISRFKGLGEMNPDQLKDTTMHPDTRRLLQVQIPE
                     GADDETRDIFVKLMGKGEAAARRAWMEREGDTAELDI"
     gene            complement(7495..8019)
                     /locus_tag="WIC03_000454"
     CDS             complement(7495..8019)
                     /locus_tag="WIC03_000454"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HEZ3883005.1"
                     /translation="MLDREGYRPNVGIILINERNEVFWGKRVREHSWQFPQGGIKPGE
                     SPETAMYRELYEEVGLLPQHVKIVGRTRDWLRYDVPNNWVRREWRGSYRGQKQIWYLL
                     RLTGRDCDVNLRATRHPEFDGWRWHQYWAPVDEVIDFKRDVYLGALKELSSRFLRGME
                     SYEDFAARQLSGNR"
     gene            8185..9480
                     /gene="serS"
                     /locus_tag="WIC03_000455"
     CDS             8185..9480
                     /gene="serS"
                     /locus_tag="WIC03_000455"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEZ3883006.1"
                     /translation="MLDIQLLRSNTAAVAERLARRGYNFDTARFDALEERRKSVQVKT
                     EELQASRNSISKQIGALKGQGKHEEAQAAMDQVAQIKTDLEQAAADLEAVQKELDAWL
                     LSIPNLPHEDVPFGKDETENVEVRKVGTPREFDFEIKDHVDLGEPLGLDFEGGAKLSG
                     ARFTVMRGQIARLHRALAQFMLDTHTLKHGYTEHYTPYIVDDTTLQGTGQLPKFAEDL
                     FHVTRGGDESKTTQYLIPTAEVTLTNTVAGSIIPSEQLPLKLTAHSPCFRSEAGSYGK
                     DTRGLIRQHQFDKVEMVQIVHPEKSYETLEEMVGHAENILKALELPYRVITLCTGDMG
                     FGATKTYDLEVWVPAQNTYREISSCSNCEDFQARRMKARFKDENGKNRLVHTLNGSGL
                     AVGRTLVAVLENHQNADGSINIPAALQPYMGGVAKLEVK"
     gene            complement(9558..10556)
                     /locus_tag="WIC03_000456"
     CDS             complement(9558..10556)
                     /locus_tag="WIC03_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HEZ3883007.1"
                     /translation="MKIAIYGTKSYDREHFTRANRHFGFELEFFDFMLDAKTAKMAEG
                     AEAVCIFVNDDGSRPVLEKLAQIGVKTVALRCAGFNNVDLKAAEELGLKVVRVPAYSP
                     ESVAEHTVGLMLTLNRRIHKAYQRTRDANFSLEGLTGFNMYGKTAGVIGTGKIGIATM
                     RILKGFGMNLLAYDPFCNPEAEKIGGRYVELNELYARSDIITLHCPATPENHYMLNEA
                     AFDKMKDGVMIINTSRGGLIDSSAAIEALKRRKIGALGMDVYENERELFFEDKSNDVI
                     TDDVFRRLSSCHNVLFTGHQAFLTEEALGNISEVTLSNIREVGQTGDCGNAVRADG"
     gene            complement(10681..11757)
                     /gene="prfA"
                     /locus_tag="WIC03_000457"
     CDS             complement(10681..11757)
                     /gene="prfA"
                     /locus_tag="WIC03_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003790927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEZ3883008.1"
                     /translation="MKPSILEKLQQLGDRLEEVTHLLGQPEATSDMDNYRKLTREHAE
                     LTPVVEVFQNYQLAQSDLADAEEMLSDPEMKDFAAEEIEAAKAKIGALDTELQKLLLP
                     KDADDDKNIFIEVRAGTGGDEAALFAGDLLRMYSRYAERNRWQVEIVSANESELGGYK
                     EVIARIIGLGAYSRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPEADELEDIELN
                     PVDLRIDTFRASGAGGQHINKTDSAVRITHLPTGMVVECQDGRSQHANKAQAMKVLAA
                     RLNDAQKREAQAKEAAERKSLIGSGDRSERIRTYNYPQGRVTDHRINLTLHKLDFVMD
                     GDLAEITDALIAEHQAELLAAMGD"
     gene            complement(11847..12617)
                     /locus_tag="WIC03_000458"
     CDS             complement(11847..12617)
                     /locus_tag="WIC03_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEZ3883009.1"
                     /translation="MRLTDTHCHLADSVLRGNLAQVLAEARQAGVWRFIVPATRPQDW
                     QSVAELSKMPSEHGQIRIALGIHPWFSDGIAERDFVRLEAMLARYPQAWVGEIGLDFY
                     DKTQTPPQRERQIQVFVRQLAIAQTLRRRVIIHNLKATADIAAAVKQTGFAQGGIVHA
                     FSGSAEEARVLTKLGFKIGIGSLLLNPNARKVRDTLKALNDGDFVLETDSPFMLKKEI
                     NTPANILRIAEIAAQIRGVGAADIAAITERNADSLLRP"
     gene            complement(12620..13612)
                     /locus_tag="WIC03_000459"
     CDS             complement(12620..13612)
                     /locus_tag="WIC03_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEZ3883010.1"
                     /translation="MKQKIFVLYTGGTIGMTQSSEGLRPDTALVSQALSPFSDGLDFE
                     WHVCNPLIDSSSVTLQHWRDWLDIIADKLPSCDGILILHGTDSMAYTANLLALALQGL
                     GKPIVLTGSQWPYAAENSDAPRNLSTAVAAFSLKLKQTVIAFDGKLYPAVGSSKVSTE
                     TAAGFDNPHFGALAEWDETQGWHNVRIPNQDAADVSDGLKIRYPDPQAKIAVRTLIPG
                     FAVQELADGLGQLPAHALILQSYGHGNTPADEGFIRAVQDFTQQGKLLLNISQVRQGR
                     TAAVYAQGNAFRNSGIINGGKCNLETATALMTLAVSEGWGADEVKHELQRLGLV"
     gene            complement(13894..14577)
                     /locus_tag="WIC03_000460"
     CDS             complement(13894..14577)
                     /locus_tag="WIC03_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEZ3883011.1"
                     /translation="MFAFLEAFFVEYGYAAVFFVLVICGFGVPIPEDLTLVTGGVISG
                     MGYTNPHIMFVVGMLGVLVGDGIMFAAGRIWGQKILRFKPIARIMTPKRYEQVQEKFD
                     KYGNWVLFVARFLPGLRTAVFVTAGISRKVSYLRFIIMDGLAALISVPIWIYLGEYGA
                     HNIDWLMAKMHSLQSGIFVILGIGATVVAWIWWKKRQRIQFYRSKLKEKRAQRKAAKA
                     AKKAAQSKQ"
     gene            complement(14766..>15137)
                     /gene="glmM"
                     /locus_tag="WIC03_000461"
     CDS             complement(14766..>15137)
                     /gene="glmM"
                     /locus_tag="WIC03_000461"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218892.1"
                     /note="catalyzes the conversion of glucosamine-6-phosphate
                     to glucosamine-1-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEZ3883012.1"
                     /translation="WLIGGEASGHILCMDKHNTGDGIISALQVLAALQILNQDLATVC
                     ADWQPYPQTMINVRIQKGQKWQEASKDVLAEVEKELEGKGRVVLRASGTEPVVRVMVE
                     ARQADWARDGAERIAAAIGGI"
BASE COUNT         3423 a         4103 c         4059 g         3552 t
ORIGIN      
        1 tcccataaaa aaacggagca gatacctgcc ccgtttttat ttaatccgaa attttaatct
       61 aaatttagaa ttttgcaccg gattggtttg ccatatagtc aacagccgct ttgacttcgt
      121 catcgctcaa acctgcattg ccgcctttgg caggcatcgc gttaaagcct tcaagggcgt
      181 gtttgtgcaa ggtttctttg ccttttttga tacgcggtgc ccaatcgtct tttttgccta
      241 tgccgggaat accgggaatc gaaccgccgt ggcacacctg acaggttgct tcgaagactt
      301 ttttaccgtc aacgccgacc gcaggggctg ccgcaccctt gtcttctgcc ttcgcttctg
      361 ccggagctgc actatcggca ggagcagaag ctgttcctga agcggcattg tcggcaggcg
      421 cagcctcatc aggattcggg aaagaaccgc cgcttttgtt cgccatgtaa gtaatcgccc
      481 gtttgagttc ctgatcggtc aggtctgccg caccgccttt ggcaggcatg gcgttaaagc
      541 cgttcagcgc gtgttggaac aaggtatcga agccttgcgc gatacgcggt gcccaatcgc
      601 cgttgtgttc cagtttcgga gcgttcggca cattgctgtc cgccgcgtgg cattggatac
      661 agattttgcc gaaaatctgt tcgccttggc gttcgccgac ggggatgccg tcgcccatcg
      721 tcaattgtcc gacaggctgg atacgggtct gcgttgctgc ttccgtagtg gcatcgacat
      781 cgccgaacga gccgctgccc gccagcttaa tcaggaaata aaggactgca ataacaataa
      841 cgataccgct cacaagggta aacagtgcag agccttgggc tttgttgtcg cggagttgtt
      901 tcatttggta ggcctcgccg tcaggttagg ttgtgctgta aattatagtt tggtgtgtta
      961 aacgcagtta acaatatttt gctggattat actgaattca cagggtcttt ccaatcgcta
     1021 tcattgaaaa tatgaaaaaa tttgccaacg gtatctgtat aaaacaaata atcatttgaa
     1081 aataatggtt tatcctcaag aaaactctcc ttatgccgcc atacgccgcc tgccggcgca
     1141 agataacctt tgccaatttg cagaatttac gttaacctta cgttttacgc acccatagct
     1201 cagttggaag agtgtcagtt tccgaagctg gaggtcacag gttcgatccc tgttgggtgc
     1261 gccaattata aagagaccgt ctgaaagata aatatttttc agacggtctt ttgacttact
     1321 tcaaactctt atttcaagac ttccgcaaat gcgcgggcaa catagtcggt attcgacgta
     1381 ttcagtccgg cgacgcacat cctgccggaa tccagcaggt aaacggcaaa ttcgtcgcgc
     1441 agcctgcgga cttgttccac gctcaatcct gtgtagccga acatgccgcg ctgtttgatg
     1501 aaataagtga aatcgcgatc ggggatttgc gcagttaaga catcataaag tttctgccgc
     1561 atcgcacgga tgcggtcgcg catcatataa acctcgtttt gccacaaggc gtaaagttcg
     1621 gggctgttca tcacgtcggc ggcgatatac gcgccgtgcg cgggcgggct ggagtagatg
     1681 cggcggacgg tgaatttgag ctgtccgaac accaaatccg cttcttcctt attcgggcaa
     1741 accacgctca ggccgccgac gcgctcgccg tagagcgaca ggttttttga gaaggaattg
     1801 ctgacgaaca agggcaattc catttccacc gctttgcgga cggcgtaggc atcgctgtcc
     1861 aaatcgccgc cgaagccttg gtaggcgatg tccataaacg gaatcagttt gcgcgttttg
     1921 atgatgtgca acacttcgtc ccattgttgt tccgacatat ccacgccggt cgggttgtgg
     1981 cagcagggat ggaggatcag gacgctgttt tcgggcaggg tgttgaaaaa cgcggtcatt
     2041 tcgtcgaatt tcacgccgac ggcagcaggg tcgtaatatg ggtaagtgcc gacctcgaaa
     2101 cctgcgcttt caaaaatgcc gcgatggttg tcccaagtcg ggtcgctgac gtaggcgcgc
     2161 gcttcgggaa accagcggtg cagaaagtcc gccccgactt tgagcgcgcc tgagccgccc
     2221 aaggtttgta cggtaacaat acgtccttgc gcgagcgcgg ggttgtcttt gccgaacaac
     2281 aaatgctgca ccgcgctgcg gtaagtgtct aagccttcca tcggcaggta gggcgacggc
     2341 acaggagtgg cggcgcgggc ggtttcggcg cggctcacgg attccaacac gggcattttg
     2401 ccttcgtcgt cgaaataaat gcctatgctc aaattgactt tttcggggcg cgggtcgttt
     2461 ttgaaggttt cgaccaaact caaaatcgga tcgccgggat agtattcgat gtgtcggtac
     2521 atagtcctta cctcttgctt tttccaagga ttttcttttt caacagcaca ccgctttcaa
     2581 tatggtgcgt aaacggaaat tgatcgaaca gggcggcacg ttcaaccgta tgggtttccg
     2641 ccaaggtgtc caaattggta cgcagcgttt cgggattgca ggaaatgtag atgatgttgt
     2701 caaactgcga caccagcttc aaagtttcct catcgatacc ggcacgcggc ggatcgacga
     2761 aaatagtgga aaatgcgtaa tccgtcaaag caataccgcc atccttaagg cgtttaaact
     2821 cacgttttcc ggtataggct tcggtaaatt cttcagcaga cagacgggcg attttgatgt
     2881 tgccgatgcg gttggcttcg atattccatt gcgccgcgct gacggaggtt ttggagattt
     2941 cggttgccaa aacacgttca aacttttcag ataaaggaag cgtaaagttg ccgtttccgc
     3001 aatacagttc gagcaaatcc ccatccaatc cttctgccgc accgcaggcc cattcaagca
     3061 ttttctgaca cacggcggca ttcggttggg taaaactgcc ttcaatttgc cgataacgga
     3121 aatcccggtt gccgaccttc aaagtttccg ttacatagtc ctgttttaag actattttct
     3181 gtcccctgct ccgcccgatg acggaaatat ccaactgttg ctgtaacgct tgcgccgcct
     3241 gcatccactc attatcaagc cttttgtggt aaatcatggt aaccagcatt tccccgctga
     3301 gcgtagacaa aaattcgacg gcataccaac ggtttctcag ttcggcagat tgggcggcaa
     3361 cggcaatcaa ttctggcatc aggcggttga cggcttcgga agccgcttca aaacggtcgc
     3421 aacggatcag gcttgccccg ccagcctttt gtcctttttc aaacatggca taaaacattt
     3481 caccgccttc gtgccaaata cggaactcgg cacgcatacg gtaatgtttg tccggagatt
     3541 cgtacacttc ccactcagga acatccaaac ctgcaaaaag ggttttaagg tggtcttttt
     3601 tatcttgaag ctgctgcgtg tagtcgctca tatcgctatc ctgaaaaatc agaccggatt
     3661 ataagccgat tcactgcaaa acacgcaacc cctttaccgg catataaccc gttttcctcc
     3721 aacttttatg ctaaaatccg ccgtctccaa cataccgccg acagaaggaa aatatggcag
     3781 gcaacacttt cggacaactc ttcaccgtga ccacgttcgg cgaaagccac ggcgcaggtt
     3841 tgggctgcat catcgacggc tgcccgcccg gcttggaatt aagcgaagcg gatatccaat
     3901 ttgacctcga ccgacgcaaa cccggcacca gccgccacgt tacccaacgc cgcgaaaccg
     3961 accaagtaga aatcctctcc ggcgtattca aaggcaaaac caccggcacg cccatcgccc
     4021 tcttaatccg caataccgac cagcgcagca aagactacgg caacatcgcc caaagtttcc
     4081 gccccggcca tgccgactat acctattggc acaaatacgg cacgcgcgac taccgcggcg
     4141 gcggcaggag ctccgcccgt gaaaccgccg cccgcgttgc tgccggagcc gttgccaaaa
     4201 aatggttgaa agaaaaattc ggcacggaaa tcaccgccta cgttacccaa gtcggcgaaa
     4261 aagaaatccg gtttgaaggc tgcgaacaca tttcccaaaa tccttttttt gccgccaacc
     4321 atagccaaat tgccgagctg gaaaactata tggacagcgt gcgcaaatcg ttggattccg
     4381 tcggcgcgaa gctgcatatt gaagccgcca atgtccccgt cggcttgggc gaacccgttt
     4441 tcgaccgcct cgatgccgaa atcgcctacg cgatgatggg catcaacgcc gtaaaaggcg
     4501 tggaaatcgg cgcaggtttt gacagcgtaa cgcaacgcgg cagcgaacac ggcgacgaac
     4561 tgaccccgca aggcttcctg tccaaccatt cgggcggcat cctcggcggc atcagcaccg
     4621 ggcaagacat ctgcgtcaat atcgccatca aacccaccag ctccatcgcc accccgcgcc
     4681 gcagtatcga catcaacggc aaccccatcg aactcgccac gcacggcagg cacgacccct
     4741 gcgtcggctt gcgcgccgcg ccgattgccg aagctatgct cgccttggtt ttaatcgacc
     4801 acgccctgcg ccatcgtgcg caaaatgcgg atgttgccgt ggatacgccc gatatcgccc
     4861 gctcggacaa gtaaaaagat ttagccaaaa cacgggcttt atactaaaat accgaccatt
     4921 gccgtgccgt ctgaacgcac gattcttaga ggaatacaac cgaaatgaca caagaaaccg
     4981 ctttgggtgc ggcactgaaa tccgccgtcc aaactatgag caaaaagaaa cagaccgaaa
     5041 tgatcgccga ccacatctac ggcaaatacg atgtgttcaa acgcttcaaa ccgttggcac
     5101 tcggcatcga tcaggatttg attgccgctt tgccgcaata cgatgccgca ctgattgcac
     5161 gcgtcctcgc caaccactgc cgccgtccgc gctatctgaa agccttggcg cgcggcggca
     5221 aacgtttcga tttgaacaac cgtttcaaag gcgaagttac ccccgaagaa caggcgatcg
     5281 cgcaaaacca tcctttcgtg cagcaggctt tgcaacagca gtccgcccaa gctgccgccg
     5341 aagcagccgg atcttccgca gcagaataaa tccccaaacg aaatgccgtc tgaaaaccga
     5401 tttggtttca gacggcattt tttcgtatgc ggcaatcacg gttcaaatat ccaattccgc
     5461 cgtatcgcct tcgcgttcca tccaagcgcg gcgggcggcg gcttcgcctt tgcccatcag
     5521 tttgacgaag atgtcgcgtg tttcgtcatc tgcgccttct gggatttgta cctgcaacag
     5581 acggcgggtg tcggggtgca tggtggtgtc tttgagttgg tcggggttca tctcgcccaa
     5641 gcctttaaaa cggctgatgg aataagcagt ttctttaaag ccttcttttt gtagtcgctc
     5701 caaaatgctg tcgagttcgt tttggtcgag ggcgtagaat ttgcgggccg gtttgttttt
     5761 gccttgtgca ttgacatcga cgcggaacag cggcggctgg gcgacgtaga tgtgtccatc
     5821 ggcgaccagt ttcgggaagt ggcggtagaa caaggtcagc agcaaaactt gaatatgcga
     5881 gccgtccacg tcggcatcgg acaggatggc gattttgccg tagcgcaggc cgcttaaatc
     5941 gggatggtcg ttaataccgt gcggatcgac gccgatggcg acggaaatgt cgtggatttc
     6001 ggcgttgccg aagagttggt cggggtggac ttcaaagctg ttgagcactt tgccgcgcag
     6061 gggcaggatg gcttgggtgg ctttgtcgcg ggcgagtttg gctgagccgc cggcggaatc
     6121 gccttcgacg aggaagagtt cgttttcgcg gatgtcttcg ctttcgcagt cggtcagttt
     6181 gccgggcagg acggcgacgc cgctgccttt tttcttttcg attttcttaa ccgaacgcat
     6241 ccgcgcctgc gcctgacgga tggcgagttc ggcgattttt ttaccgaagt ccacgttttg
     6301 gttcagccac aattccaaag ggtcgcccga tacggcggca acgagtttca acgcgtcgcg
     6361 gttggtcagc ttgtctttgg tttgaccttg gaactgcggg tcgaggacgc gggcggagag
     6421 gacgaaggcg gttttaccga acacgtcgtc actttgtact ttcacgccgc gcggtaggag
     6481 gttgtgcaga ttgatgaaat tgttgacggc gttgaacacg gcttgtttca aacctgcttc
     6541 gtgcgtgccg cccagcgggg tggggatgag gttgacgtag ctttcgttgg cgcacgagcc
     6601 ttcttccagc caggtcaggg caaacgccgc tccttcgccg atgctgaaat cgccgttgtg
     6661 gccgtctgaa atgtagtttt cgcaggagaa cagcggcaca gcttcttgcg cgtcggcaat
     6721 caggtcggtc agatagcttt tcaggccgtc ggggtagtgc caggtttggg tgtgcgcttc
     6781 gtcttcgcct ttgaccggac gggtcaggga aacgcgcaca cccggcagca gcacggcttt
     6841 ggcacgcagc aggcgttcga gttcgggaat gctgtaattc gggctttcaa aatatttgcc
     6901 gtccggccac acccgcactt cggtgccgct gtctttgacg gcgcatttgc ccacttgtgc
     6961 caacggttcg accacgtcgc cgccggcaaa cacgatacgg tggattttgc cttcgcgttt
     7021 gaccgttacc tcaaggcggg tggaaagggc gttggtcacg gatacgccca cgccgtgcag
     7081 gccgcctgaa aaggcatacg cgctgccgcc gtctttttta ttgaacttgc cgcccgcgtg
     7141 cagacgggta aacacgagtt cgactacggg tacgccttct tcagggtgca gaccgacggg
     7201 aataccgcgg ccgttgtcgc gtacggaaag cgaaccgtct tcatgaattt gcacgtcgat
     7261 tgccgtagcg aaaccgccca acgcctcgtc cgccgcgttg tcgatgactt cttggcagat
     7321 atgggtcggg ctgtcggtgc gggtgtacat accgggacgt tctttgaccg gttccaaacc
     7381 tttgaggacg gtgatgctgg attcgctgta ttggttgttt ttagccatag gaataatctg
     7441 aaagtaagaa aaacaacgct ttcagacggc ctgaaagcgt tgcgttccgt tgttttaccg
     7501 gttgccggaa agttggcggg cggcaaagtc ttcataactt tccataccgc gcaggaagcg
     7561 ggaagaaagt tctttcaatg cccccaaata aacgtcgcgt ttgaaatcaa tcacttcgtc
     7621 aacgggcgcc caatattggt gccaacgcca gccgtcaaat tcggggtggc gggtggcgcg
     7681 caggttgaca tcgcaatccc ggccggtcag gcgcaggaga taccaaatct gcttctgtcc
     7741 gcgataagag ccgcgccatt cgcggcgtac ccagttgttc ggcacgtcgt aacgcagcca
     7801 gtcgcgcgtc cgcccgacga ttttgacgtg ttgcggcaga agtccgactt cttcgtaaag
     7861 ctcgcggtac atcgcggttt cggggctttc gcccggcttg atgccgcctt gcggaaactg
     7921 ccaagaatgt tcgcgcacgc gcttgcccca aaagacttcg ttacgttcgt taatcaggat
     7981 aataccgaca ttggggcgat agccttccct gtccaacacg gtgtcgccct ccgttaaatt
     8041 caatcgtacg attttcccac aaatcgggcg gttttgacaa atcagacggc atggcggtac
     8101 gcgtaccgaa acacgggggg atttgggaaa atatcttgaa tttggtttac aataatatgt
     8161 ttcaaattat tcgggaatca gaaaatgtta gacatccaat tgctccgcag caacaccgcc
     8221 gccgttgccg aacggcttgc acggcgcggt tataactttg ataccgcacg ttttgacgca
     8281 ctggaagaac gacgcaagtc cgttcaggtg aaaaccgaag aactacaagc ctcgcgtaac
     8341 agcatttcca agcaaatcgg cgcactgaaa ggtcagggca aacacgaaga ggcacaggcg
     8401 gccatggatc aagtcgccca aatcaaaacc gatttggaac aggcagccgc cgatttggaa
     8461 gccgtgcaaa aggaactgga cgcatggctg ttgagcattc cgaacctgcc gcatgaggac
     8521 gtgcctttcg gtaaggatga aaccgaaaac gtcgaagtcc gcaaagtcgg cacgccacgc
     8581 gaatttgact ttgaaatcaa agaccatgtc gatttgggcg aacctttggg tttggatttc
     8641 gaaggtggcg cgaaactttc cggcgcacga tttaccgtga tgcgcggaca aatcgcccgc
     8701 ctgcaccgcg cgctggcgca attcatgctc gatacgcaca cgctgaaaca cggctacacc
     8761 gagcattaca caccctatat cgtggacgac accaccttgc aaggcacggg tcagctgccg
     8821 aagtttgcag aagatttgtt ccacgttacc cgtggcggcg acgagagcaa aaccacgcaa
     8881 tatctgattc cgacagctga agttaccctg accaataccg ttgccggcag cattatcccg
     8941 tccgaacaac tgccgctgaa gctgaccgcg cattcgccct gtttccgcag cgaggcgggt
     9001 tcgtacggca aagacacgcg cggtctgatt cgccagcacc agttcgacaa agtggaaatg
     9061 gttcaaatcg ttcatcccga aaaatcatac gaaacgctgg aagaaatggt cggccatgcc
     9121 gaaaacatcc tgaaggcttt ggaactgccc taccgcgtga ttaccctgtg taccggcgac
     9181 atgggcttcg gcgcaaccaa aacgtatgac ttggaagttt gggtaccggc gcaaaacacc
     9241 taccgcgaaa tctcaagctg ctccaactgc gaagatttcc aagcccgccg catgaaggcg
     9301 cgtttcaaag acgaaaacgg caaaaaccgc ttggtacata ctttgaacgg ctccggcttg
     9361 gctgtcggca gaacgctggt cgccgtattg gaaaaccatc aaaacgccga cggcagcatc
     9421 aacatccctg ccgcactgca accgtatatg ggcggcgttg ccaagttgga agtgaaataa
     9481 gtttgcaagc tgcctgaacg tcaaatgccg tctgaaacct ggttcagacg gcatttcctt
     9541 taaactttta aaacacgtca gccgtcggca cgaaccgcat tgccgcaatc gccggtctgt
     9601 ccgacctcgc ggatattgga cagcgtaact tccgaaatat tacccaacgc ctcttccgtc
     9661 aaaaacgcct gatgtccggt aaacagcacg ttatgacaag acgacaggcg gcggaacacg
     9721 tcgtcggtaa tcacatcgtt ggatttgtct tcaaaaaaca attcgcgttc gttctcgtac
     9781 acatccatgc ccaatgcgcc gattttccgg cgtttcaacg cctcaatcgc ggcagaactg
     9841 tcaatcagac cgccacggct ggtgttgata atcatcacgc cgtctttcat tttgtcgaac
     9901 gccgcttcgt tcagcatata gtggttttcc ggcgtggcgg ggcaatgcag cgtgatgatg
     9961 tccgaccggg catacagctc gttcaactcc acatacctgc cgccgatttt ttccgcttcg
    10021 gggttgcaaa acggatcgta agccagcagg ttcatgccga aaccctttaa aatccgcatc
    10081 gttgcgatac cgattttccc cgtgccgata acgcccgccg ttttgccgta catattgaaa
    10141 ccggtcagac cttccagcga aaaattcgca tcgcgggtac gctgataggc tttgtggata
    10201 cggcggttca acgtcagcat cagaccgacc gtatgttccg caaccgattc gggcgaatag
    10261 gcaggcacgc gcacgacttt caagcccaac tcttcagccg ctttcaaatc cacattattg
    10321 aagccggcac aacgcaacgc cacagttttc acgccaattt gcgccaattt ttccaacacg
    10381 ggccggctgc cgtcgtcgtt tacaaaaata caaaccgctt ccgcgccttc cgccattttc
    10441 gccgttttcg catccagcat aaaatcaaaa aactccagct cgaagccaaa atgccggttg
    10501 gcgcgggtaa aatgttcgcg gtcatagctt ttcgtaccgt aaatcgcaat cttcatcaat
    10561 atgtccagtt gtcgtctatg gttgagaaac ggcataatac actgaattca aacaaatcag
    10621 tagaatatgg tggattaaaa ttgattgcat gcacggcatt tccatttcaa aacacaaaac
    10681 tcaatcgccc attgccgcca aaagctccgc ctgatgttcg gcaatcaggg catcggtgat
    10741 ttctgccaaa tcgccgtcca tcacaaaatc cagcttgtgc agggtgaggt tgatgcggtg
    10801 gtctgttacc ctgccttggg gatagttgta ggtgcggatg cgttcgctgc ggtcgccgct
    10861 gccgatgagg gatttgcgtt cggcggcttc tttggcttgg gcttcgcgtt tttgcgcgtc
    10921 gttcaggcgg gcggcgagaa ctttcattgc ctgcgctttg ttggcgtgtt ggctgcggcc
    10981 gtcttggcat tcgaccacca tgccggtggg caggtgggta atgcggacgg cggaatcggt
    11041 tttgttgatg tgctgaccgc ctgcgccgga tgcgcggaag gtgtcgatgc gaaggtcgac
    11101 ggggttcaac tcgatgtctt ccagttcgtc cgcttcgggc atgacggcaa cggtgcaggc
    11161 ggaggtgtgg atgcggcctt ggctttcggt ggcggggacg cgctgcacgc ggtgtccgcc
    11221 cgattcaaat ttcaaacggc tgtacgcgcc taagccgata atacgggcga taacttcttt
    11281 atagccgccc aactcgcttt cgttggcgga cacgatttca acctgccagc ggttgcgttc
    11341 ggcgtagcgg ctgtacatac gcagcaaatc gccagcaaac agcgcggctt cgtcgccgcc
    11401 cgttccggcg cgtacttcga taaagatgtt tttgtcgtcg tcggcatctt tgggcagcag
    11461 cagtttttgc aattcggtat cgagtgcgcc gattttggct ttcgccgctt cgatttcttc
    11521 ggcggcaaag tctttcattt cggggtcgga caacatttct tcggcatccg ccaaatcgct
    11581 ttgcgccagt tgatagtttt ggaatacttc gacgacgggg gtcagttcag cgtgttcgcg
    11641 cgtgagcttg cggtagttgt ccatatcgga cgtggcttcg ggttgcccta aaaggtgggt
    11701 aacttcttcc aaccggtcgc cgagttgttg cagtttttct aaaatagacg gcttcataat
    11761 tcttccataa caaacgccgc ctgaagttca gacgtcatta aaacgcggtc attataatat
    11821 attttctgaa tattcaaaaa gataatctag ggacggagca ggctgtcggc attgcgttcc
    11881 gttatcgcgg cgatgtccgc cgcgccgaca ccgcgtatct gcgcggcaat ttcggcaatc
    11941 cgaagaatgt tggcaggcgt attaatctct tttttcagca taaacgggct gtcggtttcc
    12001 aatacgaaat ccccgtcgtt caaggcttta agcgtatcgc gcactttacg cgcgttcgga
    12061 ttgagcaaca acgaaccgat gccgattttg aaacccagtt tcgtcaacac gcgcgcttct
    12121 tccgcgctgc ctgagaaggc gtgaacgatg ccgccttggg caaagcctgt ctgtttgacg
    12181 gcggcggcga tgtcggcggt ggctttgaga ttatggataa tcacgcggcg gcgcagggtt
    12241 tgcgcgatgg caagctggcg gacgaaaact tggatttgcc gttcgcgctg cggcggcgtt
    12301 tgggttttgt cgtaaaaatc caagccgatt tcgccgaccc atgcctgcgg ataacgtgcc
    12361 aacatcgctt ccaggcggac gaaatcacgc tcggcaatgc cgtctgaaaa ccaaggatgg
    12421 atgccgagcg caatacggat ttgaccgtgt tcggacggca ttttcgacag ttccgccacg
    12481 ctttgccaat cctgcgggcg cgtcgcggga acgataaacc gccacacacc cgcctgtcgc
    12541 gcttcggcaa gcacctgcgc cagattgccg cgcaaaacag aatcggcaag atggcaatgt
    12601 gtgtcggtca gcctcatctt cacaccagcc ccaaccgctg caactcgtgt ttcacctcgt
    12661 ccgcgcccca tccttcagaa acggcaagcg tcatcaatgc ggttgcggtt tcgaggttgc
    12721 atttgccgcc gttgatgatg cctgagttgc ggaacgcgtt gccttgcgcg taaacggcag
    12781 cggttctgcc ttgtcggact tggctgatgt tgagcagcag tttgccttgc tgcgtaaagt
    12841 cttgcacggc gcggatgaaa ccttcgtctg cgggcgtgtt gccgtgtccg taactttgca
    12901 ggataagggc gtgggcggga agctgtccga gtccgtcggc aagttcttgg acggcaaagc
    12961 cggggataag cgtgcggacg gcgatttttg cctgcgggtc gggataacgg attttgaggc
    13021 cgtctgaaac atctgccgcg tcctgattcg ggatgcggac attgtgccag ccctgggttt
    13081 cgtcccactc cgccagtgcg ccgaaatgcg gattgtcgaa accggcggcg gtttcggtgc
    13141 tgactttgct gctgccgacg gcgggataaa gtttgccgtc aaacgcgatg acggtttgtt
    13201 tgagcttgag gctgaaggcg gcaacggcgg tggagaggtt gcgcggggca tcgctgtttt
    13261 cggcggcgta aggccattgg gaaccagtca ggacaatcgg tttgcccaaa ccttgcagag
    13321 cgagcgcgag gagattggcg gtgtacgcca tgctgtccgt gccgtgcagt atcaggatgc
    13381 cgtcgcatga agggagtttg tcggcaatga tgtccagcca atcgcgccag tgttgcagcg
    13441 taacggagga ggaatcaatc aagggattgc agacgtgcca ctcgaaatcg aggccgtctg
    13501 aaaaggggga aagggcttgg ctaaccagtg cggtatcggg gcgcaggcct tcgctgcttt
    13561 gggtcatgcc tatggtgccg cctgtgtaga ggacgaagat tttttgtttc atggacatca
    13621 tcgggtcgtc tgaaaataat aatacggctt atttaactat atttcggaca gactggcaat
    13681 ttggcggcgc ggacggtttt cagacggcct tcaaatgaaa aagcacccga gggctgtcga
    13741 tatttgattt tccaagtaga tttttattca cgaaatagga gagccgcaac aagcttaaat
    13801 cccttgtgag gttcccaaca cggaagatac cgctttgtgg attaaaaaat acggaaacta
    13861 ttgaatatcg acaacctatt taggtgcttg attttattgt ttgctttgcg cggctttttt
    13921 ggctgccttg gcggctttgc gttgcgcccg cttttctttc aatttgctgc ggtaaaactg
    13981 gatacgttgg cgttttttcc accaaatcca agcgacaacg gtcgcaccta tacccaagat
    14041 aacaaaaata cccgattgca ggctgtgcat tttcgccatc agccaatcga tgttgtgcgc
    14101 accgtattcg cccagataaa tccaaatagg gacggaaatc agcgcggcaa gcccgtccat
    14161 aatgataaaa cgcaggtatg aaaccttgcg gctgataccc gctgtaacga aaacggccgt
    14221 tctcaagccg ggcaggaagc gagcgacaaa caagacccag ttgccgtatt tgtcgaattt
    14281 ttcctgaacc tgctcataac gtttcggcgt catgatacgc gcaataggtt tgaaccttag
    14341 gattttctgc ccccaaattc gtccggcggc aaacatgatg ccgtccccga ccaatacgcc
    14401 gagcataccg actacaaaca taatatgcgg attggtataa cccatacccg aaatcacgcc
    14461 gcctgttacc aaggtcaaat cctcgggaat cggcacgccg aaaccgcaga tgaccaatac
    14521 aaaaaaaaca gccgcataac cgtattcgac aaaaaaggct tctaaaaaag caaacatggc
    14581 ggatattcca ttgtcggaga taaaaagtca gaacaaaccg aaacattttc tacatgaagc
    14641 aggcattcta tcaaagatta tgccgtctga aagcgattgc acagaaacac tcacgcccgt
    14701 atcgtgttca gacggcattt aaacaggaaa accgccgctg ttgaaacggt tttgcctgat
    14761 tttgcctaaa tgccgccgat ggcggcggca atgcgttccg caccgtcgcg cgcccaatcc
    14821 gcctgccgcg cctccaccat cacacgcacg acgggttcgg ttcccgaagc gcgcaacacg
    14881 acacgccctt tgccttcgag ttctttttcc acttccgcca acacgtcttt cgaagcttcc
    14941 tgccatttct gacctttttg gatgcgcacg ttaatcatcg tttgcggata cggctgccag
    15001 tcggcgcaaa cggtggcgag gtcttggttc aggatttgca gcgccgccaa aacttgcagc
    15061 gcggaaataa tgccgtcgcc ggtgttgtgt ttgtccatgc acaaaatatg gccgctggct
    15121 tcgccgccga tgagcca
//
LOCUS       DAWHSK010000021        14332 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.021, whole genome shotgun sequence.
ACCESSION   DAWHSK010000021 DAWHSK010000000
VERSION     DAWHSK010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14332
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.021"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(108..809)
                     /locus_tag="WIC03_000462"
     CDS             complement(108..809)
                     /locus_tag="WIC03_000462"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEZ3883013.1"
                     /translation="MSLKTVAVIGAMEQEIELLREMMENVKAVSFGKFAAYEGELAGK
                     RMVLALSGIGKVNAAVATAWIIREFAPDCVINTGSAGGLGKGLKVGDVVVGTEIAHHD
                     VDVTAFGYVWGQVPQLPAVFVSDGLLVGKAKQAAEVFECAAVEQGLIVSGDRFVHSSE
                     GVAEIRKHFPKVKAVEMEAAAIAQTCHQLETPFVIIRAVSDSADEKADISFDEFLKTA
                     AASSAKMVAEIVKSL"
     gene            995..2107
                     /locus_tag="WIC03_000463"
     CDS             995..2107
                     /locus_tag="WIC03_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEZ3883014.1"
                     /translation="MTAKEELFAWLRHMNQNKGSDLFVTTHFPPAMKLDGKITRITDE
                     PLTAEKCMEIAFSIMSAKQAEEFSSTNECNFAISLPDTSRFRVNAMIQRGATALVFRA
                     ITSKIPKFESLNLPPVLKDVALKKRGLVIFVGGTGSGKSTSLASLIDYRNENSFGHII
                     TIEDPIEFVHEHKNCIITQREVGVDTENWMAALKNTLRQAPDVILIGEIRDRETMDYA
                     IAFAETGHLCMATLHANSTNQALDRIINFFPEERREQLLTDLSLNLQAFISQRLVPRD
                     GGKGRVAAVEVLLNSPLISELIHNGNIHEIKEVMKKSTTLGMQTFDQHLYQLYEKGDI
                     SLQEALKNADSAHDLRLAVQLRSRRAQSSSPDLELL"
     gene            2141..3118
                     /locus_tag="WIC03_000464"
     CDS             2141..3118
                     /locus_tag="WIC03_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEZ3883015.1"
                     /translation="MIYPWHNEQWRQIAEHWERRPNAWLFAGKKDTGKTTFARFAAKA
                     LLCETPAPGCKPCGECMSCHLFGQGSHPDFYEITPLSDEPENGRKLLQIKIDAVREII
                     DNVYLTSVRGGLRVILIHPAESMNVQAANSLLKVLEEPPPQVVFLLVSHAADKVLPTI
                     KSRCRKMVLPAPSHEEALAYLRERGVAEPEERLAFHSGAPLFDEADGVRALRIKLLDI
                     LAEPRLLKILDYAALFDKEKLPLAVFVGWMQKWLVDLGLCLQHMKPVYYPAYEDRLLQ
                     TVSGFRPRNVFAAEDMLKQLAPYGFHTLNVKMQIEHLLINYLELKKENG"
     gene            3122..3472
                     /locus_tag="WIC03_000465"
     CDS             3122..3472
                     /locus_tag="WIC03_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilZ"
                     /protein_id="HEZ3883016.1"
                     /translation="MSDGQNIPAKMMSLQLKDMNLLYSSYMPFLEHGGLFVQTNDVFS
                     IGDDILLAVEILNFPKLFLPTKVAWINPARTSSKPKGVGLAFTKHENCLKVKDQIEVE
                     LGNTIGGSRPTFTM"
     gene            3477..4256
                     /locus_tag="WIC03_000466"
     CDS             3477..4256
                     /locus_tag="WIC03_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEZ3883017.1"
                     /translation="MHIIDSHCHLNFEGLKERLPEVLSNMEANGVGQALAISVSRESF
                     SEVFAIAEAHEHIYCTIGVHPDSKEAEEFSIAEMVAAAAHPKVVGIGETGLDYYWCKG
                     DLSWQHKRFADHIEAANQTGLPVIVHTRDAAADTLSILKECRVNSGVIHCFSEDIGFA
                     RAAMDLGLYISFSGIVTFKNAPLVQEAAKYVPDDRILVETDAPFLAPVPKRGRQNEPA
                     FVRYTAEHIAKLRNQTLEQVAAYTTENFYRLFQKVPDMRTV"
     gene            4282..4497
                     /locus_tag="WIC03_000467"
     CDS             4282..4497
                     /locus_tag="WIC03_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HEZ3883018.1"
                     /translation="MKAIHPYACPRCCRLPANTFRTGMANSASKFCIAKGGRREVKKD
                     ESGGGYALCHLPDSRIVEEWEYFRSQH"
     gene            4598..4909
                     /gene="grxD"
                     /locus_tag="WIC03_000468"
     CDS             4598..4909
                     /gene="grxD"
                     /locus_tag="WIC03_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEZ3883019.1"
                     /translation="MASIHDQIKEVVTTHRVVLFMKGTKQFPQCGFSSRAVQILNAAG
                     CTDYVTVNVLENPEVRQGIKEYSDWPTIPQLYVNGEFVGGSDILMEMYEAGELQELLK
                     A"
     gene            5015..5641
                     /gene="upp"
                     /locus_tag="WIC03_000469"
     CDS             5015..5641
                     /gene="upp"
                     /locus_tag="WIC03_000469"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEZ3883020.1"
                     /translation="MNVNVINHPLVRHKLTLMREADCSTYKFRTLTTELARLMAYEAS
                     RDFEIEKYLIDGWCGQIEGDRIKGKTLTVVPILRAGLGMLDGVLDLIPTAKISVVGLQ
                     RDEETLKPVSYFEKFVDSMDERPALIIDPMLATGGSMVATIDLLKAKGCKNIKALVLV
                     AAPEGVKAVNDAHPDVTIYTAALDSHLNENGYIIPGLGDAGDKIFGTR"
     gene            5664..5984
                     /locus_tag="WIC03_000470"
     CDS             5664..5984
                     /locus_tag="WIC03_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2322 family protein"
                     /protein_id="HEZ3883021.1"
                     /translation="MNFQDYLATFPSIDHLGGLDVQDADGKTVHHIPAVQGKLGSLKL
                     YNALAERFDGKLDKEAAEQGLIWFAEHVADARAHPGKHPNIDLLEKVVQSGETLLLKP
                     LAAQ"
     gene            6235..6657
                     /locus_tag="WIC03_000471"
     CDS             6235..6657
                     /locus_tag="WIC03_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883022.1"
                     /translation="MKKMFLSAVLLLSAAAQTVWADTVFSCKTDNNKYIEVQKINRNL
                     YEYSFGSAAKKEIAIRNSKADLSGRSDRWQGMGSGRWATMKFQNGEFMYTIWTGFDSV
                     THTESSGVVVERRGKEVARVGCTPQTAQANFNDADFSR"
     gene            6675..7436
                     /locus_tag="WIC03_000472"
     CDS             6675..7436
                     /locus_tag="WIC03_000472"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEZ3883023.1"
                     /translation="METAKPIMLIVRPSGRAKDDVETCRRAGWQAEVLSPIEIEADAA
                     GLELLSEQYARADAVFWVSPTAVETAVPYLNLSDGIKIHIAVGQGSRRALERYLGRTV
                     IAPHDGNDSEAVLRLPVWNSLPEGARVLFVRGHGGRDFLMNALQEKGFRTEMAEVYFR
                     RHKPLNFQNFQTENIAAAYITSTELVRLLFGQLPPQFSRFFKSLLYFTHHPRIAEALK
                     REGVCSVETVPTLEAALSHSSISVSDGMVFPGTSN"
     gene            7451..8785
                     /locus_tag="WIC03_000473"
     CDS             7451..8785
                     /locus_tag="WIC03_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEZ3883024.1"
                     /translation="MGEPENKSSEPVREIQASKEMPSETSSPRKENETEVHIPAAPFI
                     VKQSGSNALAVCALVLAALGLGTSGFLFVQGQNVLKNQELAFNQKIDKAALGESENAA
                     LLKDNLNRQSAIQSELDRLDSGVKANGEQILMTQKAYRELTKGRADWLVDETETILNL
                     AAQQLVLTGNIQTAVGVLEHIDSRLSRFNQAELLPIKQAVSSDLAELKNRPYVDISGT
                     ALRLDRLETAVSGLPLILDGVLKPGVQARNEAVSASWWQNVWEKSLGTLKGLVEIRRL
                     ENNDAMLLSPEQAYFVRENLRLRLLDARIALMQRNGEVYQGDLNNAEAAVRQYFDAKS
                     PATQSWLKELAELKALDVRMTADDGLKNSLNAVRAYRDGTRMTAAENQEAEQAASEPA
                     NENTASEPAAASDVKTIEAPSLPSERKPEQPAKKQSVPEKAGRSPSAKGERA"
     gene            8782..9999
                     /locus_tag="WIC03_000474"
     CDS             8782..9999
                     /locus_tag="WIC03_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis protein HemY"
                     /protein_id="HEZ3883025.1"
                     /translation="MKTVVWIVVLFAAAVGLALASGIYTGDVYIVLGQTMLRINLHAF
                     VLGSLIAVVVWYFLFKFIIGVFNIPEKMQRFGSARKGRKAALALNKAGLAYFEGRFEK
                     AELEASRVLGNKEAGDNRTLALMLGAHAAGQMANIELRDRYLAEIAKLPEKQQLSRYL
                     LLAESALNRRDYEAAEANLHAAAKMNANLTRLVRLQLRYAFDRGDALQVLAKTEKLSK
                     AGALGKSEMERYQNWAYRRQLADAADAAALKTCLKRIPDSLKNGELSVSVAEKYERLG
                     LYADAVKWVKQHYPQSRRPELLEAFVESVRFLGERDQQKAIDFADAWLKEQPDNALLL
                     MYLGRLAYGRKLWGKAKGYLEASIALKPSISARLVLAKVFDETGEPQKAEAQRNLVLA
                     SVAEENRPSAETC"
     gene            10074..11138
                     /locus_tag="WIC03_000475"
     CDS             10074..11138
                     /locus_tag="WIC03_000475"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEZ3883026.1"
                     /translation="MTLLKNDTFLRALLKQPVEYTPIWMMRQAGRYLPEYKATRAKAG
                     SFLDLCKNTELATEVTIQPLERFDLDAAILFSDILTVPDAMGLGLYFAEGEGPKFKRA
                     LQHEDDIAKLHVPDMEKLQYVFDAVSSIRKALDGRVPLIGFSGSPFTLACYMVEGGSS
                     KEFRTIKTMMYSRPDLLHKILDTNAQAVTAYLNAQIDAGAQAVQIFDTWGGVLSDEAF
                     KEFSLKYIRQIVAGLKRESEGRRVPVIVFAKGGGLWLESMAEIGADALGLDWTCNIGE
                     ARRRVGKQVALQGNFDPFALFGTPESIRTEVARILADYGQGSGHVFNLGHGINQHADP
                     EHAKILVDTVHELSRQYHGG"
     gene            11327..12706
                     /gene="radA"
                     /locus_tag="WIC03_000476"
     CDS             11327..12706
                     /gene="radA"
                     /locus_tag="WIC03_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEZ3883027.1"
                     /translation="MAKTLKTLYQCTECGGTSPKWQGKCPHCGEWNTLQESLAAPEPK
                     NARFQSWAADTSTVQSLSSVTATEVPRNPTGMGELDRVLGGGLVDGAVILLGGDPGIG
                     KSTLLLQTIAKMAQSRKVLYVSGEESAQQVALRAQRLELPTEGVNLLAEIRMEAIQAA
                     LKQHQPEVVVIDSIQTMYSDQITSAPGSVSQVRECAAQLTRMAKQMGIAMILVGHVTK
                     DGAIAGPRVLEHMVDTVLYFEGDQHSNYRMIRAIKNRFGAANELGVFAMTENGLKGVS
                     NPSAIFLASYRDDTPGSCVLVTQEGSRPLLVEIQALVDDAHGFTPKRLTVGLEQNRLA
                     MLLAVLNRHGGIACFDQDVFLNAVGGVKIGEPAADLAVILAMLSSFRNRPLPEKMVAF
                     GEIGLSGEVRPVARGQERLKEAEKLGFKRAIVPKANMPRNAKEFPNLKIYGVSSLQEA
                     IDICREDRD"
     gene            complement(12777..13256)
                     /locus_tag="WIC03_000477"
     CDS             complement(12777..13256)
                     /locus_tag="WIC03_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HEZ3883028.1"
                     /translation="MKHILPLIAASALCISTASAHPAGKPPTQNETAMTTHTLTSKYS
                     FDETVSRLETAIKSKGMNIFAVIDHQEAARRNGLTMQPAKVIVFGTPKAGTPLMVKDP
                     AFALQLPLRVLVTETDGKVRAAYTDTRALIAGSRIGFDEVANTLANAEKLIQKTVGE"
     gene            complement(13498..13857)
                     /locus_tag="WIC03_000478"
     CDS             complement(13498..13857)
                     /locus_tag="WIC03_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEZ3883029.1"
                     /translation="MNIKHLITAALIASAAFAAQAAPQKPVSAAQTAQHPAVWIDVRS
                     EQEFSEGHLHNAVNIPVDQIVRRIHEAAPDKDTPVNLYCRSGRRAEAALQELKKAGYT
                     NVANHGGYEDLLKKGMK"
     gene            complement(13896..>14332)
                     /locus_tag="WIC03_000479"
     CDS             complement(13896..>14332)
                     /locus_tag="WIC03_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEZ3883030.1"
                     /translation="LHTEDFSLKRLRDWFARDDIRLPEVCRAAAETLDFHTVNGFLNG
                     FVDMVCQDPDGNICVIDYKSNHLGTDASAYTQQAMDEAVAHHHYYLQALIYAVAAARY
                     FKLRGQPPAAVSVRYLFLRGLDGKGGGVWRWDIDAAALEQIK"
BASE COUNT         3414 a         3781 c         3964 g         3173 t
ORIGIN      
        1 aaatgccgtc tgaacaaaca gcggtgcgtc cgaatatcgg gcgcaacgtg gaaatagccc
       61 gatattctaa cggaaaaccg ctgttttggc atgatttgat aaaggtttta taaagatttg
      121 acgatttctg ccaccatttt tgcagaactt gccgccgccg ttttcaaaaa ttcgtcaaag
      181 ctgatgtctg ccttttcgtc cgccgaatcg gaaaccgcgc ggatgatgac gaaaggcgtt
      241 tccaactgat gacaggtttg ggcaatcgcc gccgcttcca tttccactgc tttgactttg
      301 gggaagtgct tgcggatttc cgccacgcct tcgctgctgt ggacgaagcg gtcgccgctg
      361 acaatcaggc cttgttctac cgccgcgcat tcaaacactt ccgccgcctg ttttgctttg
      421 ccgaccaaga ggccgtctga aacaaatacg gcgggcagtt gcggcacttg tccccaaaca
      481 tagccgaatg cggttacatc gacatcgtgg tgcgcgattt ctgtgccgac cacgacatcg
      541 ccgactttca aacccttgcc caagccgccc gcgctgccgg tgttgataac gcagtccggt
      601 gcgaattcac ggataatcca agccgttgca accgccgcgt tgaccttgcc gatgccgctc
      661 aatgcaagca ccatgcgttt tcccgctaat tcgccttcat aggcggcaaa cttgccgaaa
      721 gagacggctt tgacattttc catcatctcg cgcaaaagct cgatttcttg ttccattgcg
      781 ccgataacgg ctactgtttt caaagacata ttgctgacct gttgtgaatt tcggatagaa
      841 tgcctgatta tacacgctaa cacggcagga ttgagtggag gtggtttgtc cgtgccgtct
      901 gaaacggttt cagacggcac ggcgggtttt tggtagaatg ggaaggtaca gattgtttga
      961 agattagggg acgaggatgt ttaccgatga aaatatgacc gcaaaggaag aactgttcgc
     1021 atggctgcgc catatgaacc aaaacaaagg ttccgacctg ttcgtgacga ctcatttccc
     1081 gcccgcaatg aagctggacg gcaaaatcac ccgcatcacg gacgaaccgc tgacggcgga
     1141 aaaatgtatg gaaatcgcct tttcgattat gagtgcgaag caggcggaag aattttcatc
     1201 gaccaacgag tgcaacttcg ccatcagcct gccggacacc agccgcttcc gcgtcaatgc
     1261 gatgatacag cgcggcgcga cggcgttggt attccgtgcg attaccagca agattcccaa
     1321 gtttgaaagc ctgaacctgc cgccggtctt gaaggatgtc gcgctgaaaa aacgcgggct
     1381 ggttattttt gtcggcggca ccggctcggg caaatcgact tcgcttgcct cgcttatcga
     1441 ttaccgcaat gaaaattcgt tcggacacat catcaccatc gaagacccga tcgagtttgt
     1501 ccacgaacac aaaaactgca tcatcaccca gcgcgaggtc ggcgtggata cggaaaactg
     1561 gatggcggcg ttgaaaaaca cgctgcgtca ggcgccggat gtcatcctta tcggcgaaat
     1621 ccgcgaccgc gaaacaatgg actacgccat tgcctttgcc gaaacggggc atttgtgtat
     1681 ggcgacgctg cacgccaaca gcaccaatca ggcactcgac cgcatcatca actttttccc
     1741 cgaggagcgg cgcgaacaat tgctgacgga tttgtcgctc aaccttcagg cgtttatttc
     1801 gcaacgcctc gttccgcgag acggcggcaa gggcagggtg gcggcagtcg aggtgctgct
     1861 caattcgccc ctgatttcgg agttgattca caacggcaac atccatgaaa tcaaagaagt
     1921 gatgaaaaaa tccactaccc tgggtatgca gaccttcgat caacaccttt accaattgta
     1981 tgaaaaaggc gatatttccc tgcaagaagc attgaaaaat gccgattccg cacacgattt
     2041 gcgtttggcg gtacagttgc gcagccgccg cgcgcaaagt tccagccccg atttggaact
     2101 gctctgatgg cggtatggat ttccggacgg atggtttgaa atgatttatc cgtggcataa
     2161 tgagcaatgg cggcagattg cggaacattg ggagcgtcgt cccaatgcat ggctgtttgc
     2221 cggcaaaaaa gatacgggga aaactacatt tgcccgcttt gcggcgaagg cactgttgtg
     2281 cgaaacccct gcaccgggct gcaaaccctg tggcgaatgt atgtcctgcc atctgtttgg
     2341 acagggaagc catcccgatt tttacgaaat cacccccttg tcggacgaac ccgaaaacgg
     2401 acgcaaactg ttgcagatca aaatcgatgc cgtcagggaa atcatcgata atgtgtacct
     2461 gacttcggta cggggcggtt tgcgcgtgat tctgattcat cctgcggaaa gtatgaatgt
     2521 ccaagccgcc aacagtttgt tgaaagtgtt ggaagagccg ccgccacaag tggtcttttt
     2581 gctggtcagc cacgcggcgg acaaggtttt accgaccatt aaaagccgct gccggaagat
     2641 ggttttgccc gcgccttccc atgaagaggc attggcatat ctgcgtgaaa ggggtgtggc
     2701 ggaacctgag gaacgtctgg ctttccattc cggagcgccg ctgtttgatg aggcggacgg
     2761 tgtccgtgcg ttgcggatta aactgttgga tattttggca gaaccaaggt tgttgaagat
     2821 tttggattac gccgcgcttt tcgataagga aaaacttccg ctcgccgtat ttgtcgggtg
     2881 gatgcagaag tggctggtcg atttgggatt gtgcctgcaa cacatgaaac ccgtctatta
     2941 tcccgcttat gaagacaggc tgcttcagac ggtatccggt ttccgtccgc gcaatgtatt
     3001 tgcggcggag gatatgctca aacagcttgc cccctacggg tttcatactt taaatgtcaa
     3061 aatgcagatc gagcatctgc tcatcaacta tttggaattg aagaaagaga acgggtgaat
     3121 tatgtcagac ggacaaaata ttccggcaaa aatgatgtcg ttgcagctga aagacatgaa
     3181 tctgctgtac agctcctaca tgccgttttt ggaacacggc ggtctgtttg tgcagaccaa
     3241 cgacgtattt tccatcgggg acgatattct gcttgccgta gaaatcctca acttccccaa
     3301 actgttcctg ccgaccaaag tcgcctggat caatcctgcg cgtacttcct ccaaacccaa
     3361 aggggtaggg ctggcattca caaaacacga aaactgcctg aaagtcaaag atcagatcga
     3421 agtcgaactg ggcaacacaa tcggcggcag cagacctacg tttaccatgt aacgccatgc
     3481 atatcatcga ttcgcactgc cacctcaatt ttgaaggttt gaaagaacgc ctgcccgaag
     3541 ttttgtccaa catggaagca aacggcgtgg ggcaggcgct cgccatcagc gtcagcaggg
     3601 aaagcttctc cgaagtcttt gccatcgccg aagcgcacga acacatctat tgcaccatag
     3661 gcgtacatcc cgacagcaag gaagccgaag aattttctat tgcggaaatg gttgccgcag
     3721 ccgcccatcc gaaagtggtc ggcattggcg agacgggctt ggattattac tggtgcaaag
     3781 gcgatttgtc ctggcagcac aaacgctttg cagaccacat cgaagcagcc aatcaaaccg
     3841 gactgcccgt tatcgtccat acgcgtgatg cggcggcgga caccttgtct atcctgaaag
     3901 aatgccgggt taattcgggc gttatccact gtttttccga agacatcggt tttgcccgtg
     3961 cagcaatgga tttggggctt tatatttctt tctcgggaat cgttaccttt aaaaacgcac
     4021 ccttggttca ggaggcggcg aaatacgttc cggacgaccg cattttagtg gaaaccgatg
     4081 cgccgttcct cgcccccgta cccaagcgcg gcaggcagaa cgaaccggct ttcgtgcgtt
     4141 atactgccga acatatcgcc aaattgcgga accaaacctt ggaacaggtt gcggcataca
     4201 cgacggaaaa cttttaccgg ctgtttcaaa aagtacccga tatgcggacc gtctgaccct
     4261 gtaccgacga taaggaaaac catgaaggca attcatccgt atgcatgtcc gcgctgctgc
     4321 cggctgcctg ccaacacgtt tcggacaggc atggcaaatt ccgcttccaa attctgcatt
     4381 gccaaaggcg gcagacggga agtaaaaaaa gacgaaagcg gcggcggata tgccctgtgc
     4441 catttgccgg acagcaggat tgtcgaggag tgggaatatt tccgttcaca acattgatac
     4501 tgcgcgatat acggcaaata ttgtgggaag tttccgcttt tgcgtataat gcgtcctacc
     4561 tgacaaattt tgtcaacttt atcaaaagga ataagcgatg gcttccatcc acgaccaaat
     4621 taaagaagta gtaacgacac accgcgtcgt attgtttatg aaaggtacga agcagtttcc
     4681 gcaatgcggt ttctcttccc gcgccgtgca aatcctgaac gcggcaggct gcaccgatta
     4741 cgttaccgtc aacgtattgg aaaatcccga agtacgccaa ggcattaagg aatacagcga
     4801 ctggccgacc atcccccaac tttatgtgaa cggcgagttt gtcggcggtt cggacatcct
     4861 gatggaaatg tatgaggcag gcgagctgca agagctgctg aaagcctgat ggattcggca
     4921 atgccgtctg aacgtgtttc agacggcatt ttcttttccg gcaaatcaaa aaaaagtata
     4981 atggcgcgtc tcaaaatcac actggaacac cgcgatgaac gttaacgtta tcaaccatcc
     5041 gctcgtccgc cacaaattaa ccctgatgag ggaggcagat tgcagcacct acaaattccg
     5101 aacgcttacc acagagctgg cgcgcctgat ggcgtacgaa gcaagtcgtg attttgaaat
     5161 cgaaaaatac cttatcgacg gatggtgcgg tcagattgaa ggcgaccgca tcaagggcaa
     5221 aacattgacc gtcgttccca tcctgcgtgc cggtttgggt atgcttgacg gcgtactcga
     5281 cctgattccg actgccaaaa tcagcgtagt cggactgcag cgcgacgagg aaacgctgaa
     5341 acccgtttcc tacttcgaga aattcgtgga cagcatggac gaacgtcctg ccctgattat
     5401 cgacccgatg ctggcgacgg gcggttcgat ggttgccacc atcgaccttt tgaaagccaa
     5461 gggctgcaaa aacatcaagg cactggtgct ggttgccgcg cccgagggtg tgaaggcggt
     5521 caacgacgcg catcccgacg ttacgattta caccgccgcg ctcgacagcc acttgaacga
     5581 gaacggctac atcatccccg gcttgggcga tgcgggcgac aagattttcg gcacgcgcta
     5641 actgactgat tttcggagtt gatatgaatt ttcaagacta tctcgccaca tttccttcaa
     5701 tcgaccatct gggcggtttg gacgttcagg atgcagatgg caaaacggtt caccacattc
     5761 ctgccgttca gggtaagctc ggttcgctca agttgtacaa cgctttggca gaacgttttg
     5821 acggaaaatt ggacaaagaa gcggcagaac agggtttgat atggtttgcc gaacacgttg
     5881 ccgacgcgcg cgcccatccc ggcaagcatc ccaatatcga cctgcttgaa aaagttgtgc
     5941 aaagcggcga aaccttgctg ctcaagccgc ttgccgcgca ataattttcg accatgccgt
     6001 ctgaaatccg tttcagacgg cattttgtcg gaaagaagac cgtaaaacgg gcattttctt
     6061 ttctatttca ggatacgggc aatgatgttt caacacacag gacgacacat aaagcgttgc
     6121 cctatgtgtt gccctaattt ggaaggggtt acacccctct caaataaaat ctgatgctgc
     6181 tgccacgaag gacggatgtc cgagtggcgg ggtttcaacc attaaggaaa tacgatgaaa
     6241 aaaatgttcc tttctgccgt attgcttctg tcggctgccg cccaaacggt gtgggcggat
     6301 acggtgtttt cctgtaaaac ggacaacaac aaatatatag aagttcaaaa aatcaaccgc
     6361 aatctttacg aatattcgtt cggcagtgcg gcaaaaaaag aaattgccat acgcaacagc
     6421 aaagctgatc tgtcggggcg ttccgacagg tggcaaggta tgggcagcgg tcgttgggca
     6481 acgatgaaat tccaaaacgg cgaatttatg tacaccatat ggacaggctt cgattccgtg
     6541 actcatacgg aaagcagcgg tgtcgttgtg gagcgtaggg gcaaggaagt cgcacgggtc
     6601 ggctgtacgc cgcaaaccgc gcaggcgaat ttcaacgatg ctgatttttc ccggtaatcg
     6661 gggcggataa ggcgatggaa acagcgaaac ccatcatgct gattgtccga ccgtcgggca
     6721 gggcgaagga cgatgtggaa acctgccgtc gtgccggatg gcaggcggaa gtgttgagcc
     6781 cgattgaaat cgaagcagat gctgccggac tggaactttt gtccgaacaa tatgcccgtg
     6841 cggatgccgt gttttgggtc agtccgaccg ccgttgaaac cgccgtcccg taccttaacc
     6901 tttcagacgg cataaagata cacattgccg tagggcaggg cagccgccgc gcattggaac
     6961 gctatttggg cagaacggtc attgcgcctc atgacggaaa cgacagcgag gcggttttgc
     7021 gcctgccggt ttggaacagt ctgcccgaag gtgcacgcgt attgtttgtg cgcggacacg
     7081 gcgggcggga ttttctgatg aatgccttgc aggagaaagg ttttcggacg gagatggcag
     7141 aagtctattt cagacggcat aaacctttga actttcaaaa tttccaaacc gaaaatattg
     7201 ccgccgccta tattacgtcg accgagctgg tgcgcttgct gttcgggcag cttccgccgc
     7261 aattttcccg attcttcaaa tccttgctat actttaccca tcatccgcgc attgcggagg
     7321 cattgaagcg cgaaggcgtg tgttcggtcg aaaccgtccc tacgctggaa gccgcgcttt
     7381 cccattcttc catttccgtt tcagacggca tggtctttcc cggaacctca aattaataag
     7441 gagcaaaacg gtgggcgaac ctgaaaacaa atcatccgaa cccgtacgcg agatacaggc
     7501 atcaaaagaa atgccgtctg aaacctcttc cccacgcaaa gaaaacgaaa cagaagtaca
     7561 cattcctgcc gctcctttta tcgtcaaaca gtccggcagc aacgctttgg cagtctgcgc
     7621 cctggtattg gcggcattgg gtttgggtac aagtggtttt ttgtttgtcc aaggacagaa
     7681 tgtcttgaaa aaccaagagt tggcattcaa ccaaaaaatc gacaaagccg ctttgggcga
     7741 gtcggaaaac gccgccctgc tcaaagacaa cctcaaccgc caaagtgcca tacagtccga
     7801 actcgaccgt ttggacagcg gtgtcaaagc caacggcgaa cagattctga tgacgcaaaa
     7861 agcctatcgc gagctgacca aagggcgtgc cgattggctg gtggacgaaa ccgagaccat
     7921 actcaatctg gcggcgcaac agctggtgtt gaccggcaat atccaaacgg cagtcggcgt
     7981 gttggagcat atcgacagcc gcctgtcccg tttcaatcag gcagagcttc tgccgatcaa
     8041 acaggcagtc agcagcgact tggcggaact gaaaaaccgt ccctatgtcg atatttccgg
     8101 cacggcattg cgcctcgaca ggctggaaac cgccgtatcc ggactgccgc tgattttgga
     8161 cggcgtattg aaaccgggcg tacaggcacg caacgaagcc gtttccgctt catggtggca
     8221 gaacgtatgg gaaaaatccc tcggcacatt gaaggggctg gtcgaaatcc gccgtttgga
     8281 aaacaacgat gccatgctgc tttcaccgga acaggcatat tttgtgcgtg aaaacctgcg
     8341 cctccgcctt ttggatgcgc gcattgcatt aatgcagcgc aacggcgaag tctatcaggg
     8401 cgatttgaac aatgccgaag ccgccgtcag acagtatttc gatgccaagt ctcccgccac
     8461 gcagtcgtgg ctgaaagaac tggcggaatt gaaggcgttg gatgtgcgca tgacggcgga
     8521 tgacggtttg aaaaacagcc taaatgccgt ccgcgcctat cgcgacggta cgcgcatgac
     8581 ggcggcggaa aatcaagaag cggaacaggc ggcttccgaa ccggcaaacg aaaacacagc
     8641 ttccgaaccg gctgccgcat cggatgtgaa gaccatagaa gcaccgtccc tgccttcgga
     8701 acgcaaaccg gaacagcctg caaaaaaaca gtccgtgccg gaaaaggcag ggcgttcgcc
     8761 gtccgctaaa ggagaacgcg catgaaaacg gtagtctgga ttgtcgtcct gtttgccgcc
     8821 gccgtcggac tggcgctggc ttcgggcatt tacaccggcg acgtgtatat cgtactcgga
     8881 cagaccatgc tcagaatcaa cctgcacgct tttgtgttag gttcgctgat tgccgtcgtg
     8941 gtgtggtatt tcctgtttaa attcattatc ggcgtattca atatccccga aaagatgcag
     9001 cgtttcggtt cggcgcgtaa aggccgcaag gccgcgcttg ccttgaacaa ggcgggtttg
     9061 gcgtattttg aagggcgttt tgaaaaggcg gaacttgaag cctcgcgcgt gttgggcaac
     9121 aaagaagccg gagacaaccg tactttggca ttgatgctgg gcgcgcacgc cgccgggcag
     9181 atggcaaaca tcgagctgcg cgaccgctac cttgccgaaa tcgccaaact gccggaaaaa
     9241 cagcagcttt cccgttatct tctgctggcg gaatcggcgt tgaaccggcg cgattacgaa
     9301 gcggcggaag ccaatcttca tgcggcggcg aagatgaatg ccaaccttac gcgcctcgtg
     9361 cgtctgcaac ttcgttacgc tttcgacagg ggcgacgcgt tgcaggttct ggcaaaaacc
     9421 gaaaaacttt ccaaggcggg cgcgttgggc aaatcggaaa tggaacggta tcaaaattgg
     9481 gcataccgcc gccagctggc ggatgctgcc gatgccgccg ctttgaaaac ctgcctgaag
     9541 cggattcccg acagcctcaa aaacggggaa ttgagcgtat cggttgcgga aaagtacgaa
     9601 cgtttggggc tgtatgccga tgcggtcaaa tgggtcaaac agcattatcc gcaaagccgc
     9661 cgacccgaac ttttggaagc ctttgtcgaa agcgtgcgct ttttgggcga acgcgatcag
     9721 cagaaagcca tcgattttgc cgatgcttgg ctgaaagaac agcccgataa cgcgcttctg
     9781 ctgatgtatc tcggtcggct cgcctacggc cgcaaacttt ggggcaaggc aaaaggctac
     9841 cttgaagcga gcattgcatt aaagccgagt atttccgcgc gtttggtttt ggcaaaggtt
     9901 tttgacgaaa ccggagaacc gcagaaggcg gaggcgcagc gcaacttggt tttggcaagc
     9961 gttgccgagg aaaaccgacc ttccgccgaa acctgttgaa accttcagac ggcatcgttg
    10021 aaatgccgtc tgaaaaccat ttcacttgac aaacagactg acagaaaacc cgtatgactc
    10081 ttttaaaaaa cgataccttc ctccgcgccc tgcttaaaca gcccgtcgaa tacacgccga
    10141 tttggatgat gcgtcaggcg gggcgttatc tgcccgaata caaagccaca cgcgcgaaag
    10201 cgggcagctt cctcgatttg tgcaaaaaca ccgaattggc gaccgaagtt accatccaac
    10261 ctttggaacg tttcgatttg gacgcggcga ttttgttttc cgacatcctg accgtccctg
    10321 acgcaatggg cttgggactg tattttgccg aaggcgaagg cccgaaattc aaacgcgccc
    10381 tgcaacacga ggacgacatt gccaaattgc acgtccccga tatggaaaaa ctgcaatacg
    10441 tctttgatgc cgtcagctcc atccgtaaag cactggacgg ccgcgtaccg cttatcggct
    10501 tctccggcag cccgttcaca cttgcctgct acatggttga aggtggcagc agcaaagaat
    10561 tccgcaccat caaaaccatg atgtactcgc gccccgattt gctgcacaaa atcctcgata
    10621 ccaatgctca agccgttacc gcctatctca acgcccaaat cgacgcgggc gcgcaggctg
    10681 tgcagatttt cgacacttgg ggcggcgtct tgagcgatga ggcgtttaaa gaattcagcc
    10741 tcaaatacat ccgccagatc gtcgccggac tcaaacgcga aagcgaaggc agacgcgtgc
    10801 ctgttatcgt atttgccaaa ggcggcgggc tgtggctgga aagcatggca gaaattggcg
    10861 cagacgcatt gggcttggac tggacgtgca acataggcga agcacgccgc cgcgtcggca
    10921 agcaagtcgc cctgcaaggc aacttcgacc cgttcgccct cttcggtacg ccggaatcca
    10981 tccgcaccga ggtcgcacgc atcctagccg actacggaca aggcagcggc catgtcttca
    11041 acctcggaca cggcatcaac caacacgccg accccgaaca cgccaaaatc ttagtcgaca
    11101 ccgtacacga gctgtcgcgg cagtatcacg gcgggtaagc cggcaggaaa ctgcccgata
    11161 tgccgtttga agccgagaga tggccggtta gggtaaaaat aaggcaatgc ggcaatatcc
    11221 gccgtgtacg gatagtacat gacggcggcg gtgttgccgt attggcgcaa tcccaaccgt
    11281 ccctattttc agacggcatt tttgttttca gatgtaggga aaaccgatgg caaaaacgct
    11341 taaaaccctt taccaatgca ccgaatgcgg cggcacttcc ccgaaatggc agggcaaatg
    11401 cccgcattgc ggcgagtgga acacacttca ggaaagcttg gccgcgcccg agccgaaaaa
    11461 cgcccgcttc caatcttggg cggcggatac ctcgaccgtc caatccctct cctccgtgac
    11521 cgccaccgaa gtgccgcgca atccgaccgg catgggcgaa ctcgaccgcg tattgggcgg
    11581 cggtttggtc gatggtgcgg tcatcctgct cggcggcgac cccggcatcg gcaaatctac
    11641 gctgctgctg caaaccatcg ccaaaatggc gcaaagccgt aaagtgctat acgtttccgg
    11701 cgaagaatcc gcccaacagg tcgccctgcg cgcgcagcgt ttggaactgc ccaccgaagg
    11761 cgtgaacctg cttgccgaaa tccgcatgga agcgattcaa gcagcgttga aacagcatca
    11821 gcccgaagtc gtggttatcg attccatcca aaccatgtat tccgaccaaa tcacttccgc
    11881 ccccggttct gtgtcgcagg tgcgcgagtg tgccgcccaa ctgacgcgca tggcgaaaca
    11941 gatgggcatc gccatgatac tggtcggaca cgtgaccaaa gacggcgcga ttgccggccc
    12001 gcgcgtgctg gaacacatgg ttgataccgt gctgtatttc gagggcgacc aacattccaa
    12061 ctaccgcatg atacgcgcca tcaaaaaccg cttcggcgcg gcaaacgaac tgggcgtgtt
    12121 cgcgatgacg gaaaacggtt tgaaaggtgt gtccaacccg tccgccatct tccttgccag
    12181 ctaccgcgac gacacgcccg gttcgtgcgt tttggttacg caggaaggca gccgaccgct
    12241 tttggttgaa attcaggcat tggtcgatga cgcgcacgga ttcaccccca aacgccttac
    12301 cgtcggactg gaacaaaacc gcctcgcgat gctgctcgcc gtgttaaacc gacacggcgg
    12361 catcgcctgt ttcgatcagg atgtgttcct caacgccgtc ggtggcgtga aaatcggcga
    12421 accggcggcg gatttggcag tcatcctcgc gatgctttcc agcttccgca accgcccgct
    12481 gcccgaaaaa atggttgcat ttggagaaat cggtttgagc ggtgaagtcc gccccgtcgc
    12541 gcgcggacaa gagcggctca aagaagcgga aaaactcggc ttcaaacgcg ccatcgtccc
    12601 caaagccaat atgccgcgca acgccaaaga gtttccgaac ctgaaaatct acggcgtttc
    12661 gagtttgcag gaagccattg atatttgtcg ggaagatagg gattgaaagg aaatgccgtc
    12721 tgaaatcggg tttcagacgg catttgattt ccatccgcca tatgtcggcg acgggcttat
    12781 tcgcctacgg ttttttgtat cagtttttcg gcgtttgcca aagtgtttgc cacttcgtca
    12841 aaaccgatgc ggctgccggc gatgagggcg cgcgtatcgg tataggcggc gcgtactttg
    12901 ccgtctgttt cggtaacgag gacgcgcagg ggcagttgca gggcgaaggc ggggtctttg
    12961 accatcagcg gcgtgccggc tttgggcgtg ccgaagacga tgactttggc cggctgcatc
    13021 gttaagccgt ttcggcgggc ggcttcctga tggtcgatga cggcaaaaat gttcatccct
    13081 ttgcttttta tggcggtttc aaggcggctg acggtttcgt caaagctgta tttcgaggtg
    13141 agggtatgcg tggtcatagc ggtttcgttt tgggtgggcg gtttgccggc agggtgtgcc
    13201 gaagcggttg aaatgcagag tgcggatgcg gcaatcaggg ggagtatgtg tttcatcgta
    13261 tttccttttt cctttttggt tgaaacggta gaatcagact ttattcggga ggggtgtaac
    13321 cctttccaaa tcagggcaac acatagggcg gtgctttatg tgtcgtgaaa catcattgtt
    13381 ccttatcggt ttgtttatca ggcttcggac ggggcggcgc gcgccgcgcc cgatgttgcg
    13441 ccgcgtgccg gaacgccgta tgccgtctga aagcctgtcc tttcagacgg cattgcgtca
    13501 tttcatccct tttttgagca ggtcttcata accgccgtga ttggcaacat ttgtataacc
    13561 tgcttttttc agctcttgaa gggcggcttc ggcacgccgt ccgctgcggc agtagaggtt
    13621 gaccggcgtg tctttgtcgg gcgcggcttc gtgtatgcgg cggacgattt ggtcgacggg
    13681 gatgttgacc gcgttgtgca aatgcccttc gctaaattcc tgttcggaac ggacatcgat
    13741 ccaaacggcc ggatgttgcg cggtttgggc ggcggatacg ggtttttgcg gggctgcctg
    13801 cgcggcaaag gcggctgagg caatgagtgc ggcggtaatc aggtgtttga tattcatagg
    13861 gttttcctgc ggttgttgtc cgaaaggacg ggaagttatt ttatctgttc caaagcggcg
    13921 gcatctatgt cccaacgcca aacgccgccg cctttgccat ccaatccgcg caaaaacagg
    13981 tagcggacgg aaacggcggc gggcggttgt ccgcgcagct tgaagtagcg tgcggcggca
    14041 acggcgtaaa tcagtgcctg aaggtaatag tggtggtgtg cgacggcttc gtccattgcc
    14101 tgttgcgtgt aggcggatgc gtccgtaccg aggtggtttg atttgtagtc gatgacgcag
    14161 atattgccgt cggggtcttg gcagaccata tcgacaaagc cgtttaaaaa gccgttgacg
    14221 gtgtggaagt cgagcgtttc ggcagcggca cggcagactt cgggcagcct gatgtcgtcg
    14281 cgggcaaacc agtcgcgcag gcgtttgagg ctgaagtctt cggtgtggag gg
//
LOCUS       DAWHSK010000022        13904 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.022, whole genome shotgun sequence.
ACCESSION   DAWHSK010000022 DAWHSK010000000
VERSION     DAWHSK010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13904
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.022"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..523
                     /locus_tag="WIC03_000480"
     CDS             <1..523
                     /locus_tag="WIC03_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatB"
                     /protein_id="HEZ3883031.1"
                     /translation="LEELRKAKQEFEAAAAQVRDSLKETGTDMQNSLHDISDGLKPWE
                     KLPEQRTPADFGVDENGNPFPDAANTLSDGISDVMPSERSDTSAETLGDSGQTGSTAE
                     PAETDQDRAWREYLTASAAAPVVQTVEVSYIDTAVETPVPHTTSLRKQAISRKRGLRP
                     KHRAKPKLRVRKS"
     gene            537..1307
                     /gene="tatC"
                     /locus_tag="WIC03_000481"
     CDS             537..1307
                     /gene="tatC"
                     /locus_tag="WIC03_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEZ3883032.1"
                     /translation="MSETQNEQPVQPLVEHLIELRRRLMWTVVGILVCFFGLMPFAQQ
                     LYTFIADPLMANLPKDTSMIATDVIAPFFVPVKVTLMAAFLISLPHTLYQIWAFVAPA
                     LYQNEKRLITPLVLSSVSLFFIGMAFAYFLVFPVIFKFLAGVTPVGVNMATDIDKYLS
                     FILGMFVAFGTTFEVPIVVILLTKIGVVTTGQLKHARPYVIVGAFVIAAIITPPDVIS
                     QTLLAIPLILLYEAGIWFGRFFTPRSEQDGDIQPPATT"
     gene            1360..1968
                     /gene="maf"
                     /locus_tag="WIC03_000482"
     CDS             1360..1968
                     /gene="maf"
                     /locus_tag="WIC03_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HEZ3883033.1"
                     /translation="MNTLYLGSNSPRRMEILTQLGYRVIQLPAGIDESVKAGETPFAY
                     VQRMAEEKNRTALTLFCETNGTMPDFPLITADTCVVSDGIILGKPRSQAEAIEFLNRL
                     SGKQHTVLTAVCIRYRGKTSSRVQTNRVVFKPLSPEEISAYVQSGEPIDKAGAYAVQG
                     IGGIFIQSIEGSFSGIMGLPVYETVSMLQDLGYRSPLSALKP"
     gene            1977..2330
                     /locus_tag="WIC03_000483"
     CDS             1977..2330
                     /locus_tag="WIC03_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HEZ3883034.1"
                     /translation="MNRQTAYLLTSFSLIALIILSLSWELWIAPLRPGGSWLALKALP
                     LCLPLSGILKKKIYTYQYSSMLVLIYFAEAVMRLFDAYPAEQICAALSAVFSIIFFIS
                     CLSFVKQYKETNNAR"
     gene            2320..4134
                     /locus_tag="WIC03_000484"
     CDS             2320..4134
                     /locus_tag="WIC03_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase C-terminal domain-containing
                     protein"
                     /protein_id="HEZ3883035.1"
                     /translation="MPAETTVSGAHPAAKLPIYILPCFLWIGIVPFTFALKLKPSPDF
                     YHDAAAAAGLIVLLFLTAGKKLFDVKIPAISFLLFAMAAFWYLQARLMNLIYPGMNDI
                     VSWIFILLAVSAWACRSLVAHFGQERIVTLFAWSLLIGSLLQSCIVVIQFAGWEDTPL
                     FQNIIVYSGQGVIGHIGQRNNLGHYLMWGILASAYLNGQRKIPAALGVICLIMQTAVL
                     GLVNSRTILTYIAAIALILPFWYFRSDKSNRRTMLGIAAAVFLTALFQFSMNTILETF
                     TGIRYETAVERVANGGFTDLPRQIEWNKALAAFQSAPIFGHGWNSFAQQTFLINAEQH
                     NIYDNLLSNLFTHSHNIVLQLLAEMGISGTLLVAATLLTGIAGLLKRPLTPASLFLIC
                     TLAVSMCHSMLEYPLWYVYFLIPFGLMLFLSPAEASDGIAFKKAANLGILTASAAIFA
                     GLLHLDWTYTRLVNAFSPATDDSAKTLNRKINELRYISANSPMLSFYADFSLVNFALP
                     EYPETQTWAEEATLKSLKYRPHSATYRIALYLMRQGKVAEAKQWMRATQSYYPYLMPR
                     YADEIRKLPVWAPLLPELLKDCKAFAAAPGHPEAKPCK"
     gene            4324..5001
                     /locus_tag="WIC03_000485"
     CDS             4324..5001
                     /locus_tag="WIC03_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEZ3883036.1"
                     /translation="MSRVLLVDDDALLTELLTEYLSAEGLNVRSVPDGEAGVQEILSG
                     QYDVVVLDSMMPKMNGLDVLKNVRARSTVPIIMLTAKGDDIDRIIGLEMGADDYVPKP
                     CTPRELLARINAILRRAQHSGEQNNAPNSISVSDVVLYPAKRQASVKDMPLELTSTEF
                     NLLEVLMRHAGQVVSKETLSVEALDRKLAKFDRSIDVHISSIRHKLGDASLIQTVRGL
                     GYLFVKN"
     gene            5015..6421
                     /locus_tag="WIC03_000486"
     CDS             5015..6421
                     /locus_tag="WIC03_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEZ3883037.1"
                     /translation="MKLFQRIFATFCAVIVCAIFVASFSFWLVQNTLAENQFNQRRTI
                     ETTLMGSIISAFRARGDAGAREILTEWKDSPVSSGVYVIQGDEKKDILNRYIDSYTIE
                     RARLFAAGHPHSNLVHIEYDRFGEEYLFFTKDWDKLQARRLPSPLLIPGLPLAPIWHE
                     LIILSFIIVVGLLMAYILAGNIAKPIRILGNGMDRVANGELETRISQQVDDRDDELSH
                     LAIQFDKMVEKLEKLVAKERHLLHHVSHEMRSPLARMQAIVGLIQAQPQKQEQYLKRL
                     EGELTRMDTLAGELLTLSRLETSNMALEKESLKLLPFLGNLVEDNQSIAQKNGQTVTL
                     SADGKIPENTTILANESYLYRAFDNVIRNAVNYSPEGSTILINIGQDHKHWIIDVTDN
                     GPGVDEMQLPHIFTAFYRADSSANKPGTGLGLALTQHIIEQHCGKIIAENIKPNGLRM
                     RFILPKKKTGSKTEKSAN"
     gene            6539..8965
                     /locus_tag="WIC03_000487"
     CDS             6539..8965
                     /locus_tag="WIC03_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HEZ3883038.1"
                     /translation="MSAQTDPGYFFMPNHIILIGASERPYSLGERVLSNLLSTPFQGK
                     ITPVNPRHHTIAGLPAYTSLNKIPGNADLIIAVTLPDSYDTLFKTCRKKQLRHIILIQ
                     DWDSLSAAELHTAETAIRKHHGNGLNITACTTAGIQLPSLGLNISTQDGYAAGHTAIL
                     TGNAAVSRQIDNILNKLRQGTSRHISLHPGISPITSSDWLNRFGHSLHTKTAVLHYNP
                     EEDQRKLFSAIRQFTRHTPLILHITCRTTETDRAVLHCLARHCNFLISFNADDLEAAL
                     RAQLSDLPPLSRLDILSDTPAEWLNAHAPKTLALHFPNLPHHIRNGHLTGTPTPSICH
                     DIASRQLARPDTQAVLTILSPSGHEDYKKTARALIRLSEQTAKPLLVSSPFSDGITHF
                     DTPTQAIRTLSYRNTAAALKQAQLDIAPPQPCRLKTPQPQNIKKALAAANPSLLAEAL
                     HLPPYRHTTHNAVQFQFDSHPLYGDILTARCNGQTTAVLPPFTTLDSRHLARFAELDG
                     TQTLDQFLHTLTVIPEYRQHILGITLNLNGGQYSSDFLLKTPETHDTPKRKNTGKAAQ
                     TLEHAAAKMQSAAAYLKHKNPTAAEFLRHTSEAAAELLGSKTETGAAVPNVLAPYPAA
                     HLKTLSLKNNTTVTITPLLPEDAEAKQQFVRSLSPEARYTRFMTHTNELPAATLARLC
                     NPDYHCEAAWTARDADSNIVAVVRHSRLNRNECEFGITLAEHMRGSGLAQKMMELIIQ
                     TAAQQGYRTMSADILKTNTPMIKLAEKSGFTLKESDTEKNLYRAYLNLAADKTTEKTN
                     KNLHTDHKIT"
     gene            9010..9255
                     /gene="rpsP"
                     /locus_tag="WIC03_000488"
     CDS             9010..9255
                     /gene="rpsP"
                     /locus_tag="WIC03_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEZ3883039.1"
                     /translation="MVVIRLARGGSKHRPFYNVIVTDSRSRRDGRFIERVGFYNPVAN
                     EKQERVRLNADRLNHWIAQGAQVSDSVAKLIKEQKAA"
     gene            9271..9780
                     /gene="rimM"
                     /locus_tag="WIC03_000489"
     CDS             9271..9780
                     /gene="rimM"
                     /locus_tag="WIC03_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEZ3883040.1"
                     /translation="MTDTQNRVAMGYIKGVFGIKGWLKIAANTEYSDSLLDYPEWHLV
                     KDGKTISVTLEAGKVVNSELQVKFEGINDRDLAFSLRGYTIEIPREAFAPTEEDEYYW
                     TDLVGMTVVNKDHTVLGKVSNLMETGANDVLMIDGEHGQILIPFVSQYIETVDTGSKT
                     ITADWGLDY"
     gene            9780..10529
                     /gene="trmD"
                     /locus_tag="WIC03_000490"
     CDS             9780..10529
                     /gene="trmD"
                     /locus_tag="WIC03_000490"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEZ3883041.1"
                     /translation="MLIQAVTIFPEMFDSITRYGVTGRANRQGIWQFEAVNPRKFADN
                     RLGYIDDRPFGGGPGMIMMAPPLHAAIEHAKAQSSQAAKVIYLSPQGKPLTHQKAVEL
                     AELPHLILLCGRYEGIDERLLQSSVDEEISIGDFVVSGGELPAMMLMDAVLRLVPGVL
                     GDMQSAEQDSFSSGILDCPHYTKPLEFQGMAVPEVLRSGNHGLIAEWRLEQSLRRTLE
                     RRPDLLEKRVLIPKESRLLETIRQEQREIQS"
     gene            10545..10910
                     /gene="rplS"
                     /locus_tag="WIC03_000491"
     CDS             10545..10910
                     /gene="rplS"
                     /locus_tag="WIC03_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEZ3883042.1"
                     /translation="MNLIQQLEQEEIARLNKEIPEFAPGDTVVVSVRVVEGTRSRLQA
                     YEGVVIARRNRGLNSNFIVRKISSGEGVERTFQLYSPTVEKIEVKRRGDVRRAKLYYL
                     RGLTGKAARIKEKLPARKG"
     gene            11229..11954
                     /locus_tag="WIC03_000492"
     CDS             11229..11954
                     /locus_tag="WIC03_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010361246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEZ3883043.1"
                     /translation="MSIIVENLTVSYRRRPAVHHVDITFEEHSMWAVFGPNGAGKSTF
                     LKSLMGLQPIDTGSIRLDGLTRQNIAYLPQQSDIDRSQPMTVFDLAAMGLWYEIGFFR
                     GINAVQKQRVHEALERVGMRQFARRQIAHLSNGQFQRVLFARMLVQNAKFLLLDEPFN
                     AVDARTTYELLDVLQKCHYGGHAIIAVLHDYEQVRAYFPNTLLLAREKIAAGATETIL
                     TEPYLAQANAKMQQQESPEWCAS"
     gene            11990..12865
                     /locus_tag="WIC03_000493"
     CDS             11990..12865
                     /locus_tag="WIC03_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEZ3883044.1"
                     /translation="MNPYDLLITPFAEFDFMRYALASVFCLSLSAAPVGVFLVMRRMS
                     LIGDALSHAVLPGAAVGYMFAGLSLPAMGLGGVAAGMLMALLAGLVSRFTTLKEDANF
                     AAFYLSSLAIGVVLVSKNGSSVDLLHLLFGSVLAVDIPALQLIAAVSTLTLLTLAVIY
                     RPLVLESIDPLFLKSVGGKGGLWHVLFLVLVVMNLVSGFQALGTLMSVGLMMLPAITA
                     RLWAKHMGALILLSVLTALLCGLSGLLISYHIEIPSGPAIILCCSVLYLFSVILGKEG
                     GILTKWLKNHRHHTT"
     gene            12942..13856
                     /locus_tag="WIC03_000494"
     CDS             12942..13856
                     /locus_tag="WIC03_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEZ3883045.1"
                     /translation="MKHLKLTLIAALLTTAATAAPLPVVTSFSILGDVAKQIGGERVS
                     IQSLVGANQDTHAYHMTSSDIKKIRSAKLVLLNGLGLEAADVQRAVKQSKVSYAEATK
                     GIQPLKAEEEGGHHHDHDHDHEGHHHDHGEFDPHVWNDPVLMSAYAQNVAEALIKADP
                     EGKVYYQQRLGNYQMQLKKLHSDAQAAFNAVPAAKRKVLTGHDAFSYMGKRYHIEFIA
                     PQGVSSEAEPSAKQVAAIIRQIKREGIKAVFTENIKDTRMVDRIAKETGVNVSGKLYS
                     DALGNAPADTYIGMYRHNIKALTNAMKQ"
BASE COUNT         3584 a         4576 c         2985 g         2759 t
ORIGIN      
        1 actggaagaa ctgaggaagg caaagcagga attcgaagct gccgctgctc aggttcgaga
       61 cagcctcaaa gaaaccggta cggatatgca gaacagtctg cacgatattt ccgacggtct
      121 gaagccttgg gaaaaactgc ccgaacagcg cacgcctgct gatttcggtg tcgatgaaaa
      181 cggcaatccc tttcccgatg cggcaaacac cctatcagac ggcatttccg acgttatgcc
      241 gtccgaacgt tccgatactt ccgccgaaac ccttggggac agcgggcaaa ccggcagtac
      301 agccgaaccc gcggaaaccg accaagaccg cgcatggcgg gaatacctga ctgcttctgc
      361 cgccgcaccc gtcgtacaga ccgtcgaagt cagctatatc gatactgctg ttgaaacgcc
      421 tgttccgcac accacttccc tgcgcaaaca ggcaataagc cgcaaacgcg gtttgcgtcc
      481 gaaacaccgc gccaaaccta aattgcgcgt ccgtaaatca taaagagagc aatccggtgt
      541 ccgaaacaca aaacgaacaa cccgtccaac cgcttgtcga gcatctcatc gagctgcgcc
      601 gccgcctgat gtggacggtt gtcggcatct tagtctgctt tttcggccta atgccgtttg
      661 cccaacaact ctatactttt atcgccgacc cgctgatggc aaacctgccc aaagacacca
      721 gcatgattgc caccgatgtc atcgcaccat ttttcgtgcc ggtcaaagtt accctgatgg
      781 cggcattttt aatttcgctg ccgcatacgc tctaccaaat ctgggcattt gtcgcgcccg
      841 cactctatca aaacgaaaaa cgcctgatta ccccgcttgt cctctcaagc gtcagcctgt
      901 ttttcatcgg catggcgttt gcctattttc tggttttccc cgtcattttc aaattccttg
      961 ccggcgttac ccctgtcggc gtcaatatgg cgacagacat cgacaaatac ctgtccttta
     1021 tcttaggcat gttcgttgcg ttcggcacaa cctttgaagt tcccatcgtc gttattctgt
     1081 taaccaaaat cggcgtggta acaaccggac agctcaaaca tgcccgcccc tatgtgattg
     1141 tcggcgcgtt tgtcattgcc gccatcatca cgccgcccga tgtgatttca caaaccctgc
     1201 ttgccattcc gctgattctc ttatacgaag caggtatttg gttcggacgc tttttcacgc
     1261 cacgttcaga acaggatggc gacatacagc cgcctgcaac aacctgacac tatgccgtcc
     1321 gaacctccgc ctcataccgc aacagattaa ggaatacctt tgaataccct ctatttaggt
     1381 tcaaacagcc cgcgccgaat ggaaatcctg acacagttgg gctaccgcgt catccaactg
     1441 cctgccggca tcgacgaatc cgttaaagcc ggcgaaacac ctttcgctta cgttcaaagg
     1501 atggcagaag aaaaaaaccg aaccgccctg accctctttt gcgaaaccaa cggcacaatg
     1561 cccgatttcc ccctgattac cgccgacacc tgcgtcgttt cagacggcat tatattaggc
     1621 aaaccccgct cccaagccga agcgatcgaa tttttaaacc gattgtccgg caaacaacat
     1681 accgtcctga ctgctgtctg catccgttat cgcggcaaaa cgtcaagccg cgtccaaacc
     1741 aaccgcgttg tttttaagcc cctgagtccg gaagaaattt ccgcctatgt gcaaagcggc
     1801 gaaccgatag acaaagccgg cgcctacgcc gtacaaggca taggcggcat ctttatccaa
     1861 tctatcgaag gcagcttcag cggcattatg ggactgcccg tttatgaaac cgtttcgatg
     1921 ttgcaggatt tgggataccg ctcccccttg tccgccctta aaccgtaaag accgccgtga
     1981 acagacaaac cgcttacctc cttacctctt tcagcctgat cgcactgata atcctgtccc
     2041 tttcctggga actgtggatt gcaccgttgc gcccgggcgg ctcgtggctc gcgctcaaag
     2101 ccctccccct ctgtctgccg ctttcaggca tcttgaaaaa gaaaatctat acttaccaat
     2161 acagctccat gctggttctg atttactttg ccgaagccgt catgcgcctg ttcgacgcct
     2221 atcccgcaga acagatttgc gccgcgcttt ccgcagtatt cagcatcatc ttcttcatat
     2281 cctgcctgtc gttcgtcaaa caatacaagg aaacaaacaa tgcccgctga aacgaccgta
     2341 tccggcgcgc accccgccgc caaactgccg atttacatcc tgccctgctt cctttggata
     2401 ggcatcgtcc cctttacctt cgcgctcaaa ctgaaaccgt cgcccgactt ttaccacgat
     2461 gccgccgccg cagccggcct gattgtcctg ttgttcctca cggcaggaaa aaaactgttt
     2521 gatgtcaaaa tccccgccat cagcttcctt ctgtttgcaa tggcggcgtt ttggtatctt
     2581 caggcacgcc tgatgaacct gatttacccc ggtatgaacg acatcgtctc ttggattttc
     2641 atcttgctcg ccgtcagcgc gtgggcctgc cggagcttgg tcgcacactt cggacaagaa
     2701 cgcatcgtga ccctgtttgc ctggtcgctg cttatcggct ccctgcttca atcctgcatc
     2761 gtcgtcatcc agtttgccgg ctgggaagac acccctctgt ttcaaaacat catcgtttac
     2821 agcgggcaag gcgtaatcgg acacatcggg cagcgcaaca acctcggaca ctacctcatg
     2881 tggggcatac tcgcctccgc ctacctcaac ggacaacgaa aaatccccgc cgccctcggc
     2941 gtaatctgcc tgattatgca gaccgccgtt ttaggtttgg tcaactcgcg caccatcttg
     3001 acctacatag ccgccatcgc cctcatcctt cccttctggt atttccgttc ggacaaatcc
     3061 aacaggcgga cgatgctcgg catagccgca gccgtattcc ttaccgcgct gttccaattt
     3121 tccatgaaca ccattctgga aacctttact ggcatccgct acgaaactgc cgtcgaacgc
     3181 gtcgccaacg gcggtttcac agacttgcca cgccaaatcg aatggaataa agcccttgcc
     3241 gccttccagt ccgccccgat attcgggcac ggctggaaca gttttgccca acaaaccttc
     3301 ctcatcaatg ccgaacagca caacatatac gacaacctcc tcagcaactt gttcacccat
     3361 tcccacaaca tcgtcctcca actccttgca gagatgggaa tcagcggcac gcttctggtt
     3421 gccgcaaccc tgctgacggg cattgccggg ctgcttaaac gccccctgac ccccgcatcg
     3481 cttttcctaa tctgcacgct tgccgtcagt atgtgccaca gtatgctcga atatcctttg
     3541 tggtatgtct atttcctcat ccctttcgga ctgatgctct tcctgtcccc cgcagaggct
     3601 tcagacggca tcgccttcaa aaaagccgcc aatctcggca tactgaccgc ctccgccgcc
     3661 atattcgcag gattgctgca cttggactgg acatacaccc ggctggttaa cgccttttcc
     3721 cccgccactg acgacagtgc caaaaccctc aaccggaaaa tcaacgagtt gcgctatatt
     3781 tccgcaaaca gtccgatgct gtccttttat gccgacttct ccctcgtaaa cttcgccctg
     3841 ccggaatacc ccgaaaccca gacttgggcg gaagaagcaa ccctcaaatc actaaaatac
     3901 cgcccccact ccgccaccta ccgcatcgcc ctctacctga tgcggcaagg caaagttgca
     3961 gaagcaaaac aatggatgcg ggcgacacag tcctattacc cctacctgat gccccgatac
     4021 gccgacgaaa tccgcaaact gcccgtatgg gcgccgctgc tacccgaact gctcaaagac
     4081 tgcaaagcct tcgccgccgc gcccggtcat ccggaagcaa aaccctgcaa atgacccgcg
     4141 ccggcggatg cggataccgc ccgaaatgta aagctccatg caagacattg caaaaaacag
     4201 caaaccggta gggaaatacg ctatcaaaaa acattgcagc cgtgttaaga taaaccgtca
     4261 aacaatcttt tcacgccccg cccgaaacag ggtcggggca tacccttacg aaaaggaaac
     4321 accatgagcc gcgtattact cgtagatgac gatgccctgc taaccgaact gctgaccgaa
     4381 tacctgagcg ccgaaggtct gaacgtccgc agcgttcccg acggggaagc aggcgtacag
     4441 gaaatcctga gcgggcagta cgatgtagtc gtattggatt ccatgatgcc caaaatgaac
     4501 ggcttggatg tcttgaaaaa cgtacgcgcc cgaagcaccg tccccatcat catgctgacc
     4561 gccaaaggcg acgacatcga ccgaatcatc ggcttggaaa tgggcgcgga cgactatgtc
     4621 cccaaaccct gcacaccacg cgaactcttg gcacgcatca atgccatcct gcgccgcgca
     4681 caacacagcg gcgaacagaa caacgcaccc aacagcatct ccgtcagcga tgtcgtcctg
     4741 taccccgcca aacgccaggc atccgtcaaa gacatgccgc tcgaactgac cagcaccgaa
     4801 ttcaacctgc tcgaagtcct gatgcgccat gccggacagg tagtcagcaa agaaaccctg
     4861 tccgtcgaag cactcgaccg caagctggca aaattcgacc gcagtatcga cgtacacatc
     4921 tccagcatcc gccacaagtt gggcgatgcc tctctgattc aaaccgtacg cggcttgggc
     4981 tacctgtttg tcaaaaactg aaataaacag ataaatgaaa ctgttccaac gcatcttcgc
     5041 cacattttgc gcggttatcg tctgtgcaat ctttgtggcg agtttttctt tctggctggt
     5101 gcagaacacc cttgccgaaa accagttcaa ccaacgccgc accatcgaaa ccactttgat
     5161 gggcagcatc atttccgcat tccgggcacg cggggacgcg ggcgcgcgcg aaatcctgac
     5221 ggaatggaaa gacagccccg tctcatcggg cgtgtacgtt atacagggcg acgagaaaaa
     5281 agatatcctg aaccggtata tcgacagcta taccatcgaa cgcgcccggc ttttcgccgc
     5341 cggacacccg cattccaacc tcgtccatat cgaatacgac cgcttcggcg aagaatacct
     5401 gttcttcacc aaagactggg acaaactcca agcccgccgc ctgcccagcc ccctgttgat
     5461 ccccggcctg ccgctcgccc cgatttggca cgaactcatc atattgtcct tcatcatcgt
     5521 cgtcggactg ctgatggcgt acatcctcgc cggcaatatc gccaaaccca tcagaatctt
     5581 aggcaacggc atggacaggg tggcaaacgg agaacttgaa acccgtatct cccaacaggt
     5641 cgacgaccgc gacgacgaat tgtcccatct tgccatccaa ttcgacaaaa tggtggaaaa
     5701 actcgaaaaa ctcgttgcca aagaacgcca cctgctccat cacgtttccc acgaaatgcg
     5761 ctccccgctt gcccgtatgc aggcaattgt cggactgatt caggcgcagc cccaaaaaca
     5821 ggagcaatat ctcaaacggc tggaaggcga actgacccgc atggatacgc tggccgggga
     5881 actgttaacc ctgtcccgtc tcgaaacttc caatatggct ttggaaaaag aaagcctgaa
     5941 actcctgccc ttcctgggca acctggtaga agacaatcaa agcattgccc agaaaaacgg
     6001 acaaacggtt accctgtctg ccgacggaaa aatccccgaa aacacaacca tccttgccaa
     6061 cgaaagctac ctgtaccgcg ccttcgacaa cgtcatccgc aacgccgtca actacagtcc
     6121 cgaaggcagc accatcctga tcaacatcgg acaagaccac aaacactgga taatcgacgt
     6181 taccgacaac ggccccggcg tggacgaaat gcagctcccg cacatcttca ccgctttcta
     6241 ccgtgcagac tccagtgcca acaaacctgg aacaggactg gggcttgcat tgacccaaca
     6301 tattattgaa cagcactgcg gaaaaatcat cgccgaaaac atcaaaccga acggtctgcg
     6361 gatgcgcttc atcctaccca agaaaaaaac cggttccaaa acagaaaaaa gtgcgaactg
     6421 accataatgc cgtctgaacc ggcttcagac ggcattgcac aaacagttac cccaaaacaa
     6481 acacacagcg ggtgcatctc caacaatcac gcacataccg tccgcaaagg aatatatcat
     6541 gtcggcacaa accgatccgg gctacttttt catgcccaac cacattatcc tgataggcgc
     6601 gagcgaacgt ccgtacagcc tgggcgaacg cgtactcagc aacctgctga gtacgccctt
     6661 tcaaggaaaa atcacccccg taaacccgcg ccaccacacc atagctgggc ttcccgccta
     6721 caccagcctc aacaaaatcc ccggcaatgc agacctgatt attgccgtta ccctacccga
     6781 cagttacgac accctcttca aaacctgccg caaaaagcag ctccgacaca tcatcctcat
     6841 acaggactgg gacagcctgt ctgccgcaga actgcacacc gccgaaactg ccatccgcaa
     6901 acaccacggc aacggactca acatcaccgc ctgcaccacc gcaggcatcc aactgccctc
     6961 actcggactc aacatcagta cccaagacgg atacgccgca ggccataccg ccatactgac
     7021 cggcaatgcc gccgtcagcc gccaaatcga caacatcctg aacaaactcc gtcaaggcac
     7081 atcccgccac atcagcctgc atcccggcat cagccccatc acttcctcag actggctcaa
     7141 ccgcttcggc cacagcctgc acaccaaaac cgccgtccta cactacaacc ctgaagagga
     7201 tcagcgcaaa ctgttcagcg caatccgcca atttacccgc catacgccgc tgattctcca
     7261 catcacctgc cgcacgacag aaaccgaccg tgccgtactg cactgcctcg cccgacactg
     7321 caacttcctc atcagtttca acgccgacga cctcgaagcc gcactgcgcg cccaactgtc
     7381 cgaccttcct cccctgtccc gactcgacat cctgtccgac acgcctgccg aatggctgaa
     7441 cgcgcacgcg ccaaaaaccc tcgccctcca ctttcccaac cttccccatc acatccgcaa
     7501 cggacacctg accggcacac ccacaccctc aatctgccac gacatcgcct cacgtcagct
     7561 tgcccgcccc gacacccaag ccgtcctaac catcctcagt ccctccggac acgaggatta
     7621 caaaaaaaca gcacgcgccc ttatccgcct gtccgaacag accgccaaac ccctgctcgt
     7681 cagcagcccc ttttcagacg gcataacaca tttcgacacc cccactcagg caatccgcac
     7741 cctttcctac cgcaacaccg ccgccgccct gaaacaggca cagctcgaca ttgcaccgcc
     7801 gcagccatgc cgtctgaaaa caccgcaacc ccaaaacatc aaaaaagccc ttgcagcggc
     7861 aaacccctcc ctgctcgccg aagccctgca cctccccccc taccggcaca ccacccataa
     7921 cgccgtacaa ttccaattcg acagccaccc cctctatggc gacatcctga ccgcacgctg
     7981 caacggacaa accactgccg tactcccgcc gtttaccacg ctcgacagcc gccaccttgc
     8041 ccgctttgcc gaactcgacg gcacacaaac cctcgaccag ttcctgcaca cactgaccgt
     8101 cattcccgaa taccgccaac acattctcgg catcaccctc aacctcaacg gcggacaata
     8161 cagcagcgat ttcctcttaa agacacccga aacacacgac acgcccaaac gcaagaacac
     8221 aggcaaagcc gcccaaaccc tcgaacatgc cgccgcaaaa atgcagagtg ccgccgcata
     8281 cctgaaacac aaaaacccga cagccgccga atttctccgc cacacaagcg aagccgccgc
     8341 agaactgctc ggcagcaaaa ccgaaaccgg agcagccgta cccaacgtac ttgcccccta
     8401 tcccgcagca cacctcaaaa cactgtccct aaaaaacaac acgaccgtta ccattacccc
     8461 ccttttgccc gaagacgcag aagccaaaca gcagttcgtc cgcagcctca gccccgaagc
     8521 acggtacaca cgcttcatga cccacaccaa cgaactgccc gcagccacgt tggcacgcct
     8581 gtgcaacccc gactaccact gtgaagccgc atggacggca agggatgccg acagcaacat
     8641 cgtcgccgtc gtccgccaca gccgcctgaa ccgcaacgaa tgcgaattcg gcatcacact
     8701 ggcggaacat atgcgcggca gcggactggc acagaaaatg atggaactca tcatccaaac
     8761 cgccgcacag caaggctacc ggactatgag tgccgacatt ctcaaaacca atactcccat
     8821 gatcaaactt gctgaaaaat caggatttac cctcaaggaa tcggacaccg aaaaaaacct
     8881 gtaccgcgca tatctgaacc ttgcggcaga caaaacaaca gaaaaaacaa ataaaaactt
     8941 gcacaccgac cacaaaataa cctaaaatcg gcagtttcca tatatccgat tttctcaaaa
     9001 ggactcaaaa tggtagttat ccgtttggca cgcggcggct cgaaacaccg ccctttctac
     9061 aacgtcatcg ttactgactc acgcagccgc cgcgacggcc gcttcatcga acgcgtaggc
     9121 ttctacaacc ccgtagccaa cgaaaaacaa gagcgcgtcc gcctcaacgc agaccgcctg
     9181 aaccactgga ttgcacaagg cgcgcaagtc agcgactccg ttgcaaaact gattaaagaa
     9241 caaaaagccg cctaatccac atttgccgcc atgacagaca ctcaaaaccg ggtagccatg
     9301 ggctacatca aaggcgtatt cggcataaaa ggctggttga aaattgccgc caacaccgaa
     9361 tattccgaca gccttttgga ctaccccgag tggcatttgg tcaaggacgg caaaaccatc
     9421 agcgttaccc ttgaagccgg aaaagtcgtc aacagcgaac tccaagtcaa attcgaaggc
     9481 ataaacgacc gcgacttggc attctcattg cgcggttaca ccatcgaaat accccgtgaa
     9541 gcattcgccc cgacagaaga agacgaatac tactggacag acttggtcgg catgaccgtc
     9601 gtcaacaaag accataccgt tttaggcaag gtaagcaacc tgatggaaac cggcgcaaac
     9661 gacgtattga tgattgacgg agaacacggg cagattctga ttccgttcgt ttcccaatat
     9721 atcgaaaccg tcgataccgg cagcaagacc attactgccg actggggttt ggactactga
     9781 tgcttatcca ggcagttacc attttccccg aaatgttcga cagcattacc cgctacggcg
     9841 taacgggacg cgcgaacaga cagggaatct ggcagtttga agcagtcaat ccccgaaagt
     9901 ttgccgacaa cagattgggc tacatcgacg accgcccgtt cggcggcggc ccgggaatga
     9961 ttatgatggc tccgccgctt catgcagcaa tagaacacgc caaagcacaa tcctcccaag
    10021 ctgcaaaagt catctacctc agcccccaag ggaaaccgct gacacaccaa aaagcggtag
    10081 aactggcaga acttccgcat ctgattctgc tgtgcggacg gtatgagggc atagacgaaa
    10141 ggcttctgca aagcagcgtc gatgaagaaa tcagcatcgg agacttcgtt gtttccggcg
    10201 gagagcttcc cgccatgatg ctgatggatg cggtattgag gctcgtaccc ggcgtattgg
    10261 gcgatatgca gtctgccgaa caagattcgt tctcaagcgg cattttggac tgcccccact
    10321 acaccaaacc cttagaattt caaggcatgg ctgttccgga agtattacgt tccggcaatc
    10381 atggcttgat agcggaatgg cggttggaac aatcgctgcg ccgcaccttg gagcgcagac
    10441 ccgatctttt ggaaaagcgc gttttaatcc caaaggaatc ccgcctctta gaaaccatcc
    10501 ggcaagagca acgggaaatc caatcataat ttaggaaaaa aacaatgaac ctgattcaac
    10561 agctcgagca agaagaaatt gcccgcctga acaaagaaat ccccgaattc gcaccgggcg
    10621 acaccgtagt cgtatccgta cgcgtcgtag agggtacccg tagccgtctg caagcctacg
    10681 aaggcgttgt cattgcacgc cgcaaccgtg gtctgaacag caacttcatc gtccgcaaaa
    10741 tctccagcgg cgaaggtgtt gaacgtactt tccaactgta ctctccgacc gtcgaaaaaa
    10801 tcgaagtcaa acgccgtggt gacgtacgcc gtgccaaact gtactacctg cgcggcctga
    10861 ccggcaaagc tgcacgcatc aaagaaaaac tgcctgcacg caaaggttga ttcaaacccg
    10921 ttttccccaa tgtcgtctga accttctgac ggcatttttt atcgccgcaa tatcaacaag
    10981 acaacgatat tcccaatccg cctcccctgc ttaacaagca ctcccgtcta ttcaaacaaa
    11041 atttacagac caaacgttat acagtatcat agccgccata taacgataac cgctttgcca
    11101 acttcccttt caaaaccgca aatcattatg acaccttccc tcttactgtc aggattgacc
    11161 ttccgactct tcctcgccct gattgccgta tcccttttat ggggcgttta cttttgggca
    11221 gtatccgcat gagcatcatt gtcgaaaacc tgaccgtcag ctaccgccgc cgacctgccg
    11281 tgcaccatgt ggacattact tttgaagaac atagtatgtg ggcggttttc ggtcccaacg
    11341 gcgcagggaa atctaccttt cttaaatcct tgatgggatt gcagcccatc gacactggca
    11401 gcatccggct ggacggattg acccgtcaga acattgccta ccttccccag caatccgaca
    11461 tcgaccgctc gcagcccatg accgttttcg acttggcggc aatggggcta tggtatgaaa
    11521 tcggtttttt cagaggaata aatgccgttc aaaaacaacg cgttcacgaa gccttggagc
    11581 gtgtaggaat gcggcagttt gcccgccgcc aaatcgccca cctctcaaac ggacaattcc
    11641 aacgcgtcct tttcgcccga atgctggttc aaaacgccaa attcctactt ctggacgaac
    11701 ctttcaacgc tgttgatgca cggacaacct acgagcttct cgacgtgttg caaaaatgcc
    11761 attacggcgg acacgctatc attgccgttt tgcacgatta cgaacaagtc cgtgcctact
    11821 ttcccaatac cctgctgctc gcccgcgaaa aaattgcggc aggtgcaacc gagaccattc
    11881 tgacagaacc ctacctcgct caagccaacg ccaaaatgca gcaacaggaa agccccgagt
    11941 ggtgcgcctc ataaatgccg tctgaaacca tgtccccgta ccaaacacta tgaaccccta
    12001 cgacctgctt atcacaccct ttgcagaatt cgactttatg cgctacgccc tcgcatccgt
    12061 cttctgcctg tccctcagtg ccgcacccgt cggcgtattc ctcgtcatgc gccgtatgag
    12121 cctgataggc gacgcattga gccacgccgt cctgcccggt gccgccgtcg gctacatgtt
    12181 tgccggctta agcctgcccg ccatgggttt gggcggcgta gccgcaggta tgctgatggc
    12241 actgcttgcc ggactcgtca gccgcttcac caccctgaaa gaagatgcca actttgccgc
    12301 cttttatctc agcagcctcg ccatcggtgt agtcctcgtc agcaaaaacg gcagcagcgt
    12361 cgatttgctc cacctccttt tcggctccgt acttgccgtc gatattcctg ccctgcaact
    12421 catcgccgcc gtatccaccc tcacactgct tacccttgcc gtcatctacc gcccgctcgt
    12481 actcgaaagc atcgaccccc tgtttctcaa atctgtcggc ggcaaaggcg ggctttggca
    12541 cgtcctcttt ctcgtcctgg tcgtcatgaa cctcgtatcc ggctttcaag ccctcggcac
    12601 actcatgtcc gtcggactta tgatgctgcc agccattacc gcccgcctat gggcgaagca
    12661 catgggcgca ctcatcctcc tatccgttct gacagccctg ctgtgcggct tgagcggact
    12721 gctcatttcc taccacatcg aaattccttc cggtcccgcc atcatcctct gttgcagcgt
    12781 cctttatctc ttttccgtca tactcggcaa agaaggcggc attctgacca aatggctcaa
    12841 aaaccaccgc caccacacca cctgaccccg caagccgctt gaacaaacag gcagcttgtt
    12901 ttaccataac agatgttacg ttatatcaca ggagaacagt tatgaaacac ctcaaactca
    12961 cccttattgc cgcattgcta accaccgccg caactgccgc ccccctgccc gtcgtaacca
    13021 gcttcagcat tttaggcgac gttgccaagc aaatcggcgg agagcgcgta tccatacaaa
    13081 gtttggtcgg agccaaccaa gatactcatg cctatcacat gaccagtagc gacattaaaa
    13141 aaatccgcag tgcaaaactc gtcctgctca acggcttagg acttgaagct gccgatgtac
    13201 aacgtgccgt caaacagagc aaagtatcct atgccgaagc gaccaaaggc atccaacccc
    13261 tcaaagccga agaagaaggc ggacaccatc atgaccacga ccatgaccac gaaggacacc
    13321 accatgacca cggcgaattt gacccgcacg tctggaacga ccccgtcctt atgtccgcct
    13381 atgcccaaaa cgtcgccgaa gccctgataa aggccgaccc cgaaggcaaa gtttattatc
    13441 aacaacgctt gggcaactac caaatgcagc ttaaaaaact gcacagcgac gcacaagccg
    13501 catttaacgc cgtccctgcc gccaaacgca aagtcctgac cgggcacgat gccttttcct
    13561 acatgggcaa acgttaccat atcgaattca tcgccccgca aggcgtgagc agcgaagccg
    13621 agccctcagc caaacaagtc gccgccatca tccgacaaat caaacgcgaa ggcatcaaag
    13681 ccgtctttac cgaaaacatc aaggacaccc gtatggttga ccgtatcgcc aaagaaaccg
    13741 gtgtcaacgt cagcggcaaa ctgtattccg acgcactcgg caacgcgccc gcagacacct
    13801 acatcggaat gtaccgccac aacatcaaag ccttgaccaa cgcgatgaag caataaaacc
    13861 gtttgattgg tttgaatttg aaaaggcaaa aggccgtctg aaaa
//
LOCUS       DAWHSK010000023        13755 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.023, whole genome shotgun sequence.
ACCESSION   DAWHSK010000023 DAWHSK010000000
VERSION     DAWHSK010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13755
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.023"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..495)
                     /locus_tag="WIC03_000495"
     CDS             complement(<1..495)
                     /locus_tag="WIC03_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEZ3883046.1"
                     /translation="MKKIQADVVVIGGGTAGMGAFRNARLHSDNVYLIENNVFGTTCA
                     RVGCMPSKLLIAAAEARHHALHTDPFGVHLDKDSIVVNGEEVMQRVKSERDRFVGFVV
                     ADVEEWPADKRIMGSAKFIDEHTVQIDEHTQITAKSFVIATGSRPVILPQWQSLGNRL
                     IINDD"
     gene            complement(744..1481)
                     /locus_tag="WIC03_000496"
     CDS             complement(744..1481)
                     /locus_tag="WIC03_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEZ3883047.1"
                     /translation="MALQDRTGQKVPSVVFRTRVGDTWKDVSTDDLFKGKKVVVFSLP
                     GAFTPTCSSSHLPRYNELFGAFKENGVDAIYCVSVNDTFVMNAWAAEEESDNIYMIPD
                     GNGEFTEGMGMLVGKEDLGFGKRSWRYSMLVNDGVVEKMFIEPEEPGDPFKVSDADTM
                     LQFVAPDWKAQESVAIFTKPGCQFCAKAKQALQDKGLSYEEIVLGKDATVTSVRAITG
                     KMTAPQVFIGGKYIGGSEDLEAYLAKN"
     gene            complement(1760..4036)
                     /gene="metE"
                     /locus_tag="WIC03_000497"
     CDS             complement(1760..4036)
                     /gene="metE"
                     /locus_tag="WIC03_000497"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEZ3883048.1"
                     /translation="MTTLHFSGFPRVGAFRELKFAQEKYWRKEISEQELLAVAKDLRE
                     KNWKHQVAANADFVAVGDFTFYDHILDLQVATGAIPARFGFDSQNLSLEQFFQLARGN
                     KDQFAIEMTKWFDTNYHYLVPEFHADTEFKANAKHYVQQLQEAQALGLKAKPTVVGPL
                     TFLWVGKEKGAVEFDRLSLLPKLLPVYVEILTALVEAGAEWIQIDEPALAVDLPKEWV
                     EAYKDVYATLSKVSAKILLSTYFGSVAEHAALLKALPVDGLHIDLVRAPEQLDAFADY
                     DKVLSAGVIDGRNIWRANLNKVLETVEPLQAKLGDRLWISSSCSLLHTPFDLSVEEKL
                     KANKPDLYSWLAFTLQKTQELRVLKAALNEGRDSVAEELAASQAAADSRANSSEIHRA
                     DVAKRLADLPANADQRKSPFADRIKAQQAWLNLPLLPTTNIGSFPQTTEIRQARSAFK
                     KGELSAADYEAAMKKEIALVVEEQEKLDLDVLVHGEAERNDMVEYFGELLSGFAFTQY
                     GWVQSYGSRCVKPPIIFGDVSRPEAMTVAWSTYAQSLTKRPMKGMLTGPVTILQWSFV
                     RNDIPRSTVCKQIALALNDEVLDLEKAGIKVIQIDEPAIREGLPLKRADWDAYLNWAG
                     ESFRLSSAGCEDSTQIHTHMCYSEFNDILPAIAAMDADVITIETSRSDMELLTAFGEF
                     QYPNDIGPGVYDIHSPRVPTEAEVEHLLRKAIEVVPVERLWVNPDCGLKTRGWKETLE
                     QLQVMMNVTRKLRAELAK"
     gene            complement(4174..5052)
                     /gene="metF"
                     /locus_tag="WIC03_000498"
     CDS             complement(4174..5052)
                     /gene="metF"
                     /locus_tag="WIC03_000498"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEZ3883049.1"
                     /translation="MNYAKEINALNNSLSDLKGDINVSFEFFPPKNEQMETMLWDSIH
                     RLQTLHPKFVSVTYGANSGERDRTHGIVKRIKQETGLEAAPHLTGIDASPDELRQIAK
                     DYWDSGIRRIVALRGDEPAGYEKKPFYAEDLVKLLRSVADFDISVAAYPEVHPEAKSA
                     QADLINLKRKIDAGANHVITQFFFDVERYLRFRDRCVMLGIDVEIVPGILPVTNFKQL
                     GKMAQVTNVKIPSWLSQMYEGLDDDQGTRNLVAASIAIDMVKVLSREGVKDFHFYTLN
                     RSELTYAICHILGVRP"
     gene            5252..5527
                     /locus_tag="WIC03_000499"
     CDS             5252..5527
                     /locus_tag="WIC03_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEZ3883050.1"
                     /translation="MKPNIHPDNYRTVLFFDSSANEGWLIRSCAETHGKTMVWTDGKE
                     YPLFSLDTSSASHPVYTGKQRNVNTEGRASKFNQRFQSVMSSFRKDK"
     gene            5527..5652
                     /gene="ykgO"
                     /locus_tag="WIC03_000500"
     CDS             5527..5652
                     /gene="ykgO"
                     /locus_tag="WIC03_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HEZ3883051.1"
                     /translation="MQVLSSLKTAKQRHRDCQIVRRRGKVYVICKSNPRFKARQR"
     gene            complement(5757..5957)
                     /locus_tag="WIC03_000501"
     CDS             complement(5757..5957)
                     /locus_tag="WIC03_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883052.1"
                     /translation="MIGRLFRIFFFFALAALIINRLFSRRQKRALHDIAKISAWVLLG
                     AAAATLFWYLVMLYFKHIPDSY"
     gene            6115..7254
                     /locus_tag="WIC03_000502"
     CDS             6115..7254
                     /locus_tag="WIC03_000502"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEZ3883053.1"
                     /translation="MSQNASVGIVTPQKIPFEIPLVLENGKTLPRFDLMIETYGELNA
                     EKNNAVLICHALSGNHHVAGRHSAEDKYAGWWDNMVGPGKPIDTERFFVVGLNNLGGC
                     DGSSGPLSINPETNREYGADFPVVTVKDWVKSQAALADYLGIGQWAAVVGGSLGGMQA
                     LQWAISYPERVRHALVIASAPKLSTQNIAFNDVARQAILTDPDFNEGHYRSRNTVPAR
                     GLRIARMMGHITYLAEDGLGKKFGRDLRSDGYQYGYGVEFEVESYLRYQGDKFVGRFD
                     ANTYLLMTKALDYFDPAADFGNSLTRAVQDVQAKFFVASFSTDWRFAPERSHELVKAL
                     IAAQKSVQYIEVKSAHGHDAFLMEDEAYMRAVAAYMNNVYKECQQ"
     gene            7251..7832
                     /gene="metW"
                     /locus_tag="WIC03_000503"
     CDS             7251..7832
                     /gene="metW"
                     /locus_tag="WIC03_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HEZ3883054.1"
                     /translation="MNLRDDLQLIYDRIPEGSRVLDLGCGDGELLAALVEHKKCSGYG
                     IEIDTNSVIAAMSRGVNVIQADLEEGLTAFNDQSFDVIVLSQTIQAMQNTEKILRCLM
                     RVAKQAIVSFPNFGYWRNRVQIALGGHMPVSERMPYHWYDTPNIHWCTLKDFDLLCAK
                     NNIRVLERAVMTGNRQVKHFPNLLGSLAFYRVG"
     gene            7949..8794
                     /locus_tag="WIC03_000504"
     CDS             7949..8794
                     /locus_tag="WIC03_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="d-alanine-poly(phosphoribitol) ligase"
                     /protein_id="HEZ3883055.1"
                     /translation="MKNKTSSLLLWLAAIMLTACSPSKEDKTKENGASAASSTASAAS
                     SSAPQTDLQPAASAPDNVKQAENVPPSNCTDLHPATGIDDLMQQIAEHIDSDCLFALS
                     HHELETRFGLPDSGYDNIQRLLFPDIRPEDPDYHQKIILAIEDLRYGKRTISRQAQDA
                     LMEQERRLREATLLLIQGSQETRGQGEEPKRTRYFEVSATPAYSSRHNNGLGGNFQYI
                     GQLPGYLKIHGEMLENQSLFRLSNRERNPDKPFLDIHFDENGKITRIVVYEKNIYFNP
                     NLGRR"
     gene            complement(8844..9404)
                     /gene="efp"
                     /locus_tag="WIC03_000505"
     CDS             complement(8844..9404)
                     /gene="efp"
                     /locus_tag="WIC03_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEZ3883056.1"
                     /translation="MKTAQELRAGNVFMVGNDPMVVQKTEYIKGGRSSAKVSMKLKNL
                     LTGAASETIYKADDKFDVVILSRKNCTYSYFADPMYVFMDEEFNQYEIEADNIGDALK
                     FIVDGMEDQCEVTFYEGNPISVELPTIIVREVEYTEPAVKGDTSGKVMKTARLVGGTE
                     IQVMSYIENGDKIEIDTRTGEFRKRA"
     gene            complement(9447..10595)
                     /gene="earP"
                     /locus_tag="WIC03_000506"
     CDS             complement(9447..10595)
                     /gene="earP"
                     /locus_tag="WIC03_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HEZ3883057.1"
                     /translation="MNTPPFVCWIFCKVIDNFGDIGVSWRLARVLHRELGWQVHLWTD
                     DVSALRALCPDLPDVPCVHQDIHVRTWHSDAADIDTAPVPDVVIETFACDLPENVLHI
                     IRRHKPLWLNWEYLSAEESNERLHLMPSPQEGVQKYFWFMGFSEKSGGLIRERDYCEA
                     VRFDSEALRQRLMLPEKNAPEWLLFGYRSDVWAKWLEMWRQAGSPMTLLLVGTQIIDS
                     LKQSGVIPQDALQNDGDVFQTASVRLVKIPFVPQQDFDKLLHLADCAVIRGEDSFVRA
                     QLAGKPFFWHIYPQDENVHLDKLHAFWDKAHGFYTPETARAHRRLSDDLNGGEALSAT
                     QRLECWQILQQHQNGWRQGAEDWSRYLFGQPSASEKLAAFVSKHQKIR"
     gene            complement(10614..10892)
                     /locus_tag="WIC03_000507"
     CDS             complement(10614..10892)
                     /locus_tag="WIC03_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883058.1"
                     /translation="MSRNLLVRWLVVCLIPLATLAVFAANPPEDKPQHLINGIILACE
                     ATFLFKFVLFDTIKHHLKQEFDLKRQTMLLFIPIVLLVVYLFHYFGAF"
     gene            11241..12182
                     /gene="miaA"
                     /locus_tag="WIC03_000508"
     CDS             11241..12182
                     /gene="miaA"
                     /locus_tag="WIC03_000508"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEZ3883059.1"
                     /translation="MPTPKAFALLGPTAGGKTALALKIAETLPVEIISLDSALVYRDM
                     DIGTAKPSASERAFVPHHLIDIITPTESYSAARFVEDCTRLVGEISLRGRFALIVGGT
                     MMYFRALTQGLNDLPEADACLRADLDEQKQMYGLDFLYRTLQKVDPETACRLKPNDSQ
                     RIERALEVYYLTGKPMSEHLGRQSPHTLPFDLHTAALIPENRARLHENIALRFHLMLE
                     QGFIGEVENLRRRYPGLTADSPAIRCVGYRQAWKYLDGKTDFPAFVEKGIAATRQLAK
                     RQLTWLRKTPLDCVADPFSDGTSGTRLIEAAKRFFGA"
     gene            12186..12905
                     /locus_tag="WIC03_000509"
     CDS             12186..12905
                     /locus_tag="WIC03_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEZ3883060.1"
                     /translation="MLTTPPLAPKTVAALHRLGIRTLEELRQNGSVKAFLLLKASGLT
                     LTKSTLWQLESLLDGTPPQEMSQAHKAYLLAELKNHPPVTAFPPQEEMEHFMREALRQ
                     AKQSAADGEIPVGAVIVSDGKIIASAHNTCIADCNVSRHAEINALAQAGSEMQNYRLD
                     GCDIYITLEPCAMCASALIQARIKRVIYGAAEPKTGAAGSIVNLFADKRLNTHTAIRG
                     GILQEECRAVLSRFFQNKRKG"
     gene            12910..13398
                     /locus_tag="WIC03_000510"
     CDS             12910..13398
                     /locus_tag="WIC03_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HEZ3883061.1"
                     /translation="MTVLTVILALALIAVGTAGIVYPALPGLALMFAGTWLLAYSGGY
                     QIYGAGILWTVGLISLAGILADYVAGIWGTKYTGAGRLAVRGALAGSIVGIFFSLPGL
                     VLGPFIGAAAGELIDRRNMLQAGKAGLGTLLGLIVGTAFKIGCAVSILFILLVKYIAY
                     LF"
     gene            13464..>13755
                     /locus_tag="WIC03_000511"
     CDS             13464..>13755
                     /locus_tag="WIC03_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEZ3883062.1"
                     /translation="MANQRPIYGFHAVNARLWQNPKSIVELYIQEGKSDARTREVLEK
                     AANENIRVYFADADRLNAISKGARHQGVVGFIDASKNHVHLEDVLENLSEPPL"
BASE COUNT         3380 a         3617 c         3516 g         3242 t
ORIGIN      
        1 gtcatcgttg ataatcaaac gattgcccaa agactgccat tgcggcagga tgacgggacg
       61 cgaaccggta gcaatcacga aactttttgc cgtaatttga gtatgctcgt cgatttggac
      121 ggtatgctcg tcgataaatt tagccgaacc catgatgcgc ttgtcggcag gccactcttc
      181 cacatcggca acgacaaagc cgacaaaacg gtcacgctcg gatttgacgc gctgcatgac
      241 ctcttcaccg ttgacgacga tgctgtcttt gtccaaatgc acgccgaacg ggtcggtatg
      301 caatgcgtga tgacgcgcct ctgcggcggc aatcaagagt ttggaaggca tacagcccac
      361 gcgcgcgcag gtcgtgccga acacattgtt ttcaatcagg taaacattat ccgaatgtaa
      421 acgggcattg cgaaacgcac ccataccggc agtaccgccg ccgattacga cgacatccgc
      481 ttgaattttt ttcataatct ttgtccttcc gttttcgtct gaaacattgc cgacacagaa
      541 catatcggtt tcagacggca tttaaatatt ttcccaatca aaaacatcat tcagataaat
      601 aaaaacaaaa tttcgattga gtgcaacaat atccaaattc tcttataaat atagagttat
      661 tcggagcggt agtgtagaac cgcccgaata actctatccg gtatatcaga cagtttaatt
      721 aaaccgcctt aagcaaacag ctatcagttt ttagccaagt aagcttccaa atcttcgctg
      781 ccgccgatgt atttaccgcc gatgaagact tgaggggcag tcatcttgcc ggtaatggcg
      841 cgaacggaag tgacggttgc atctttgccc aatacgattt cttcgtaaga caaacctttg
      901 tcttgcaaag cttgtttggc tttagcgcag aattggcaac ctggtttagt gaaaattgcc
      961 acagactctt gagccttcca atcgggagca acgaattgca gcatagtatc tgcatcggat
     1021 actttgaacg gatcgcccgg ttcttcaggt tcgatgaaca ttttttcaac cacgccgtcg
     1081 ttaaccagca tggagtaacg ccaagagcgt ttaccgaagc ccaagtcttc tttaccgacc
     1141 agcataccca taccttcggt aaattcgccg ttgccgtcag gaatcatgta gatgttgtcg
     1201 gattcttctt cggcagccca agcgttcatt acgaacgtat cgtttacaga tacgcagtag
     1261 attgcgtcaa cgccgttttc tttgaacgcg ccgaacaatt cgttgtaacg cggcaggtgt
     1321 gaagaagaac aagtcggggt aaatgcaccg ggcagggaga atacgactac ttttttgcct
     1381 ttgaacaaat catcggtaga cacatctttc caagtgtcgc cgacgcgggt gcggaatact
     1441 acggaaggta ctttttgacc ggtacgatct tgcaaagcca ttttaagctc cttaatagat
     1501 tggtttgaga aataattgtg aatcgctggg cattctaccc catgcaaaat gatttgtata
     1561 atttatagtt caaataatat aaattattta aaactatcaa acacaactcc gatttgcctg
     1621 tttcccacat ataaagccga attagaaaaa aacaagctgc aatgaaaaga ttttccggca
     1681 actgcgccct tgcgccgcaa atcaggacaa aataccgtct gaaaggcttt cagacggtat
     1741 ttattcatac ggtctcggct tatttcgcca attcggcacg cagttttcgg gttacgttca
     1801 tcattacttg gagttgttcc agagtttctt tccagccgcg tgttttcagg ccgcagtccg
     1861 ggttaaccca cagacgttca accggtacaa cctcgatggc tttgcgcaac aggtgctcca
     1921 cttcggcttc tgtcggtacg cgcgggctgt ggatgtcgta aacccccggg ccgatgtcgt
     1981 tcgggtattg gaattcgccg aacgcggtca agagttccat gtcggaacgt gaagtctcga
     2041 tggtgatcac gtccgcatcc attgcagcaa tcgcaggcag gatatcgttg aactcggagt
     2101 aacacatatg agtgtggatt tgggtgctgt cttcgcaacc ggcagaggac aggcggaagg
     2161 attcgcccgc ccagttcagg taggcatccc aatcggcgcg tttcagcggc aagccttcgc
     2221 ggatggcagg ttcgtcaatt tggatgactt tgatgccggc tttttccaga tccaatactt
     2281 cgtcgttcag agccagtgcg atttgtttgc acacggtaga gcgaggaatg tcgttgcgga
     2341 cgaaagacca ttgcagaatg gttacagggc cggtcaacat acctttcatc gggcgtttgg
     2401 tcaggctttg tgcgtaagta gaccaagcca cggtcatggc ttcaggacgg cttacgtcgc
     2461 caaagataat cggtggtttc acgcagcgtg agccgtagct ttgtacccag ccgtattgag
     2521 tgaatgcaaa accgctcaac aattcgccga agtattcaac catgtcgtta cgctcggctt
     2581 cgccgtgtac cagtacgtcc aagtccagtt tttcttgctc ttcaaccacc aaggcgattt
     2641 cttttttcat cgcggcttcg taatcggcgg cagacagttc gccttttttg aaggctgagc
     2701 gtgcctggcg gatttcggtg gtttgcggga aagaaccgat gttggtagtc ggtagcagag
     2761 gtaggttcaa ccatgcttgt tgcgctttga tacggtcggc aaatggagat ttgcgttggt
     2821 ctgcgttggc aggcaaatcg gccaggcgtt tggcaacgtc tgcacgatgg atttcgctgc
     2881 tgttggcacg ggagtcggca gcagcttggc tggcggcgag ttcttcggca acagaatcac
     2941 ggccttcgtt caatgcagct ttcagaacgc gcaattcttg ggttttttgc agggtgaatg
     3001 ccaaccaaga gtacaggtcg ggtttgttgg ctttcagttt ttcttcaact gacaagtcaa
     3061 atggagtgtg cagcagcgag caagagctgg aaatccacaa acggtcaccc agtttggctt
     3121 gcagaggctc gacagtttcc aaaactttgt tcaggttggc gcgccaaatg ttgcggccgt
     3181 caatcacgcc ggcagacagg actttgtcgt agtcggcgaa cgcgtccagt tgctcggggg
     3241 cgcgtaccaa gtcgatgtgc agaccgtcaa caggcagggc tttcaacaat gcggcgtgtt
     3301 cggcaacaga accgaagtaa gtgctcaaca ggattttggc acttactttg ctcaaagtag
     3361 cgtaaacgtc tttgtaggct tccacccatt ctttaggcaa atcgacagcc aaagcaggct
     3421 cgtcgatttg aatccactcg gcaccggctt caaccaaagc agtcaggatt tcaacgtaaa
     3481 caggcaacag tttaggcaac aggctcagac ggtcgaattc gacggcgcct ttttctttac
     3541 ccacccacag gaaagtcaac ggacctacaa cggtcggttt ggctttcaga ccgagggctt
     3601 gggcttcttg cagttgttga acatagtgtt tggcattggc tttgaattcg gtatcggcgt
     3661 ggaattcagg caccaagtag tggtagttgg tgtcgaacca tttggtcatt tcgatagcga
     3721 attggtcttt gttaccgcgc gccagttgga agaattgttc caaagacagg ttttggctgt
     3781 cgaagccgaa gcgggcggga atcgcgccgg tggcgacttg caggtcgagg atgtggtcgt
     3841 agaaagtgaa atcgcctacg gcaacgaaat cggcgttggc agcgacctgg tgtttccagt
     3901 ttttctcgcg caagtcttta gcaacagcca gcaattcttg ctcgctgatt tctttgcgcc
     3961 agtatttttc ttgtgcgaat ttcaattcgc ggaaggcgcc gacacgcggg aagcctgaga
     4021 aatgtaatgt tgtcatgttg aactctccag ttggattttg taaggaccgg ttcaatccga
     4081 atcggccggt attgtaaatc agaatggctc ggatggggcg gtgttttgct ttttagacac
     4141 cttagatgcc gtctgaaact gtttgatacg gctttaaggg cgcacgccta aaatatggca
     4201 aatggcgtaa gtcagctcgc tgcggttaag cgtatagaag tggaaatctt tcacgccttc
     4261 gcgggacagg actttgacca tatcgatggc gatacttgcc gccaccagat tgcgcgtacc
     4321 ttggtcgtcg tccaaacctt catacatttg cgacagccag cttgggattt tgacgttggt
     4381 tacttgagcc attttaccga gctgcttgaa gttggtaaca ggcaaaatac cggggacgat
     4441 ttccacatcg atacccaaca tcacgcagcg gtcgcggaag cgcaggtagc gttccacatc
     4501 gaagaagaat tgggtgatga cgtggttcgc gcccgcatcg attttgcgtt tcaaattaat
     4561 caggtcggct tgtgcggatt tcgcttcggg atgcacttcg ggatatgctg ctacagagat
     4621 gtcgaagtcg gcgacggagc gtaatagctt aaccaagtct tcggcgtaaa acggtttttt
     4681 ctcataaccg gccggctcgt ctccgcgcag ggcgacaatg cggcggatgc cgctgtccca
     4741 ataatctttg gcaatttggc gcaattcgtc gggagaagcg tcgataccgg tcaggtgagg
     4801 cgcggcttcc aagccggttt cctgtttgat gcgtttgacg atgccgtgtg tgcggtcgcg
     4861 ctcgcctgag tttgcaccgt aagttacgga aacaaatttc gggtgcaagg tttgcaggcg
     4921 atggatggaa tcccacagca tggtttccat ttgttcgttt ttcggcggga aaaattcgaa
     4981 tgaaacgttg atgtcgcctt tcaaatcgga aaggctgtta tttaacgcat tgatttcttt
     5041 tgcgtaattc atggctgcgt accttttatt atcttttata tgatgtttca ggcaacataa
     5101 taaagttgcg ccaaacatga gtcaatttca aaattttcag atttatcatg aaaatttcta
     5161 atatattaca aaaatagtta aatttccttg tacagccctt ctaaactatg ctagagtttg
     5221 ttatgttata acactgaaag caaggcaata tatgaaaccg aatattcatc ccgacaatta
     5281 ccgaaccgtc ctttttttcg atagcagcgc caatgaaggc tggttaatcc gctcctgtgc
     5341 cgaaacacac ggaaaaacga tggtttggac agacggcaaa gaatatccgc tgttttcgct
     5401 ggatacttct tccgcatccc atcccgtcta tactggcaaa cagcgcaacg tcaataccga
     5461 aggccgtgcc agcaaattca accaacgttt ccaatccgtc atgtcatcgt ttagaaagga
     5521 caaataatgc aggttctgtc ttcactcaaa actgccaaac aacgccaccg cgactgccaa
     5581 atcgtcagac gcaggggcaa agtttacgtc atctgcaaaa gcaatccgcg ttttaaagcg
     5641 cgccaacgct gaccccctac ccttcctact tcggttgtaa aacccctcct accgcaagcc
     5701 cttttcaaat atgccgtctg aaacagaaaa atgcgtttca gacggcatat tttccgtcaa
     5761 tacgaatcag gaatgtgttt gaaatacagc atgaccaaat accaaaacag cgtcgcggcg
     5821 gctgcgccaa gcagtaccca tgcgctgatt ttggcgatat cgtgcagggc acgtttttgc
     5881 ctgcggctga aaaggcggtt aataatcagc gcggcaagtg cgaaaaagaa aaaaatacgg
     5941 aaaagtctgc ctatcatata gggaagcggt ggaggtcgtc tgaaaaagta tggatcggtt
     6001 gcggcggcat ggggacggat gtttcagcgc gtcgggctgc ttgtccgacc gtgtcaaaaa
     6061 gtcatgccgt ttgctatata ataacgcttt tcgccgcatt tgttggaaca ggatatgagt
     6121 caaaatgcct cggtgggcat tgtaacgccc caaaaaattc cgtttgagat accgctggtt
     6181 ttggaaaacg gtaaaacttt gccgcgtttc gatctgatga ttgaaaccta cggcgagctg
     6241 aatgccgaaa aaaacaatgc ggttttaatc tgccacgcgc tgtcgggcaa ccatcacgtt
     6301 gcgggcaggc attcggcgga ggataaatat gcgggctggt gggacaatat ggtcggtccc
     6361 ggcaaaccga ttgatacgga acgatttttc gtggtcggtt tgaacaatct gggcggctgc
     6421 gacggcagca gtgggcctct gtcgattaac cctgaaacca accgagaata cggcgcggat
     6481 tttccggtgg ttacggtgaa ggattgggta aaatcccaag ccgcgcttgc cgattatctc
     6541 ggcattggac aatgggcggc ggttgtcggc ggcagtttgg gcggtatgca ggctttgcag
     6601 tgggctattt cctatcccga gcgcgtgcgc catgctttag tgattgcgtc tgcgccgaag
     6661 ctgtccacgc aaaatatcgc gtttaacgat gtggcgcgtc aggcgatttt gactgacccc
     6721 gatttcaacg aaggacatta ccgcagccgc aacaccgttc ctgcacgggg cttgcggatt
     6781 gcccgtatga tgggacacat tacctatctt gccgaagacg gtttgggcaa aaaattcgga
     6841 cgcgatttgc gttccgacgg ctatcaatac ggttatggcg ttgaatttga agtagaatcc
     6901 tatctccgct atcagggcga caaattcgtc gggcggtttg acgccaacac ctatttactg
     6961 atgaccaagg ctttggacta tttcgatccg gcggcggatt tcggcaacag cctgacccgc
     7021 gccgtgcagg atgttcaggc aaaattcttt gtcgccagct tcagcaccga ctggcgtttc
     7081 gcgcccgaac gttcgcacga actggtcaag gccctgattg ccgcccaaaa atccgtgcag
     7141 tatatcgaag tcaaatccgc acacgggcac gatgcctttt taatggaaga cgaagcctat
     7201 atgcgtgcgg tcgccgccta tatgaacaac gtttataagg aatgtcagca atgaacctgc
     7261 gcgatgattt gcaactgatt tacgaccgga tacccgaagg cagccgcgtc ttggacttgg
     7321 gctgcggcga cggcgaactg cttgccgcct tggtcgaaca caaaaaatgc agcggctacg
     7381 gcatcgaaat cgacacaaat agcgtgattg ccgcgatgtc gcgcggcgta aatgttatcc
     7441 aagccgattt ggaagaaggt ttaaccgcat tcaacgatca aagttttgat gtgattgtgt
     7501 tgagccagac catccaagcg atgcagaata ccgaaaaaat cctgcgctgc ctgatgcgcg
     7561 ttgccaaaca ggcaatcgtc agcttcccaa atttcggcta ctggcgtaac cgcgtccaaa
     7621 tcgcactcgg cggacatatg ccggtttccg aacgtatgcc ctaccattgg tatgacacgc
     7681 ccaatatcca ttggtgcacg ctcaaagatt ttgatttgtt gtgcgccaaa aacaacatcc
     7741 gcgtcctcga gcgcgccgtg atgacgggca acagacaggt caaacatttc cccaaccttt
     7801 tgggcagcct tgcgttttac cgcgtcgggt aagacaggtt gagaaccttg tcggcaataa
     7861 caggttcttc ggttctatta acaacaggcc gtctgaaaaa tgaaatggca ggttttcaga
     7921 cggcctcttt aatattggaa acaatagaat gaaaaacaaa acctcatcac ttctcttatg
     7981 gcttgccgca atcatgctga ccgcgtgttc cccgagcaaa gaagataaaa cgaaagaaaa
     8041 cggcgcatcc gccgcctcgt ccacggcatc cgccgcttcg tcttccgcgc cccaaaccga
     8101 tttgcaaccg gccgcatccg cccctgataa cgtcaagcag gcagaaaacg tgccgccgtc
     8161 aaattgcacc gacctgcacc ccgccaccgg cattgacgat ctcatgcagc aaatcgccga
     8221 acacatcgac tcggactgtc tgtttgccct ttcccatcac gaactggaaa cccgtttcgg
     8281 cttacccgac agtggctatg acaacataca gcggctgctg tttcccgaca tccgccctga
     8341 agatcccgac taccatcaga aaatcatact ggcaattgaa gacttgcgtt acggaaagcg
     8401 cacgatcagc cggcaggcac aagatgcctt gatggaacag gaacgccgcc tccgagaagc
     8461 gacgctgttg ctgatacagg gcagtcaaga aacccgcgga caaggcgagg agccgaaacg
     8521 cacgcgttat tttgaagttt cggcaacccc tgcctattcg agccggcaca acaacggact
     8581 tggcggcaat ttccaataca tcggccaatt gcccggctat ctgaaaatac acggagaaat
     8641 gcttgaaaac caatcactct tccggctgtc caaccgtgaa cgcaatcccg acaaaccgtt
     8701 tttagacatc cattttgacg aaaatggcaa aatcacgcgt attgtcgttt acgaaaaaaa
     8761 catctacttc aatccaaact tggggcgaag ataaaaaaat gccgtctgaa acgttttcag
     8821 acggcatttt tcaatgcggc aaatcaggcg cgtttgcgga attcgccggt gcgggtgtcg
     8881 atttcgattt tgtcgccgtt ttcgatgtag gacatcactt ggatttcggt gccgccgacc
     8941 agacgcgcgg ttttcatcac tttgccggaa gtatcgcctt taacggcagg ctcggtgtac
     9001 tcgacttcgc gcacgatgat ggtgggcagt tcgacagaaa tgggattgcc ttcgtagaag
     9061 gtaacttcgc attggtcttc cataccgtca acgatgaatt tcaacgcgtc gccgatgttg
     9121 tcggcttcga tttcgtattg gttgaattct tcatccataa agacgtacat cgggtcggca
     9181 aagtagctgt aagtacagtt tttgcgggac aggatgacca cgtcgaattt gtcgtcggct
     9241 ttgtaaatgg tttcggaagc cgcgccggtc agtaggtttt tcagtttcat gctgactttg
     9301 gcggaagagc ggccgccttt gatgtattcg gttttttgaa cgaccatagg atcgttgccg
     9361 accataaata cattgccggc gcgcagttct tgtgctgttt tcattgtgat tttccaatca
     9421 aatcggttgt cgtaaaacgc gctattctag cgtatttttt gatgctttga aacaaaggcg
     9481 gcgagttttt cggatgcgga aggctgcccg aaaagataac ggctccaatc ctccgcgcct
     9541 tgccgccagc cgttttgatg ttgttgcagg atttgccaac attcgaggcg ttgtgttgcg
     9601 gataaagcct ctccgccgtt gaggtcgtct gaaaggcggc ggtgcgcccg ggcggtttcg
     9661 ggcgtgtaga aaccgtgtgc cttatcccaa aaggcgtgga gtttgtcgag atggacattc
     9721 tcgtcttgcg gatagatgtg ccaaaagaag ggtttgcccg caagctgggc gcgcacgaaa
     9781 ctgtcttcgc cgcgtatcac ggcgcagtcg gcgaggtgca gcagtttgtc gaagtcctgt
     9841 tgcggcacga aagggatttt gacgaggcgg acggatgccg tctgaaaaac atcgccgtcg
     9901 ttttgcaggg catcttgcgg aataacgccg ctttgtttga ggctgtcgat gatttgcgtc
     9961 cccaccagca acagtgtcat cgggctgcct gcctgtcgcc acatttccag ccactttgcc
    10021 caaacatcgc tccgatagcc gaaaagcagc cattcggggg cgtttttttc gggaagcatc
    10081 agccgctgac gcagggcttc gctatcgaaa cggacggctt cgcagtaatc gcgttcgcgt
    10141 atcaacccgc cgcttttttc gctgaaaccc ataaaccaaa aatatttttg aacaccttcc
    10201 tgcggcgaag gcatcaggtg cagcctttca ttgctttcct ccgcgctcaa atattcccaa
    10261 ttcagccaaa gcggcttgtg tcggcggata atgtgcagca cattttcggg caggtcgcag
    10321 gcaaaagttt cgatgacgac atcgggaaca ggcgcggtat caatatctgc cgcatcggaa
    10381 tgccaagtgc ggacatgaat atcctgatga acgcagggaa catcgggcaa atcagggcaa
    10441 agcgcacgca aggcggacac atcgtccgtc cacaaatgca cctgccaacc gagttcgcgg
    10501 tgcaaaacac gggcgagccg ccacgaaacg ccgatgtcgc cgaaattgtc gatgaccttg
    10561 caaaaaatcc aacagacaaa aggaggagta ttcatagaaa taacggaaac gggctaaaac
    10621 gcgccgaaat agtggaacaa atacacaacc agcaaaacaa tcggaataaa cagcaacatg
    10681 gtttgacgtt tcaaatcaaa ctcttgtttc aaatgatgct tgatggtgtc gaaaaggacg
    10741 aatttgaaca aaaacgtcgc ttcgcaggca aggatgatgc cgttgatcag atgctggggt
    10801 ttgtcttcgg gcggattggc ggcgaaaacg gcaagcgtcg ccaaagggat gaggcagacg
    10861 acaagccagc ggacaagcag gtttcgggac attgcaaggc tttcaaaaaa gcaaaacgcc
    10921 attatcgccc caagcgcacg ccttgtctaa cggatgcgcc gacgatgccg tctgaagcct
    10981 tgccgcagga aaacaccgca ccgccgaacc tttaaatacg cccgagacgg gcgttccccg
    11041 aaaaatcgga cgctgccgcc gatgcctgag gacaaggctg catcgggcgc gggcatattg
    11101 aaaacccggc gcgtcggttt gaaattttat aaaggcatac ggtaaacacg cccccttgcc
    11161 gtcggcgtgc ttccgcaaac caaaatataa cgtcggcacg ctttccgcta taatccgtat
    11221 ttttccaaac ctgaaacatt atgccgaccc cgaaagcctt tgccctgctc gggccgaccg
    11281 ccggaggcaa aaccgcgctc gccctcaaaa ttgccgaaac cctgcctgtc gaaatcatca
    11341 gcctcgattc cgcgctggtc taccgcgaca tggacatcgg cacggcgaaa ccgtccgcct
    11401 ccgaacgcgc ctttgtcccg caccacctta tcgacatcat tacgcctacc gaatcataca
    11461 gcgccgcccg ctttgtcgaa gactgcacgc gcttggtcgg ggaaatttcc ttacgcggca
    11521 ggtttgccct tattgtcggc ggcacgatga tgtatttccg cgccctgacc caaggtttga
    11581 acgatttgcc cgaagccgac gcctgcctgc gtgccgactt ggacgaacaa aaacagatgt
    11641 acggcttgga tttcctctac cgcaccctgc aaaaagtcga ccctgaaacc gcatgccgtc
    11701 tgaaaccgaa cgacagccag cgtatcgaac gcgctttgga agtttattat ctgaccggca
    11761 aaccgatgag cgaacacctc ggcagacaaa gcccccatac gctccccttc gatttgcata
    11821 ccgccgccct gattcccgaa aaccgcgccc gcctgcatga aaacatcgcc ctgcgtttcc
    11881 acctgatgct tgaacagggc tttatcggcg aagtagaaaa cctgcgccgc cgctatcccg
    11941 gcctgaccgc cgactccccc gccatccgct gcgtcggcta ccgtcaggca tggaaatatc
    12001 tggatggaaa aaccgatttc ccggcatttg tcgagaaagg cattgccgcc acccgccaac
    12061 ttgccaaacg ccaactgact tggttgcgca aaacaccttt agactgcgtt gccgacccgt
    12121 tttcagacgg cacttcaggc acgcgcctga ttgaggcggc aaaacggttt ttcggcgcat
    12181 aaaccatgct gaccacgccg ccgctcgccc ccaaaaccgt cgccgccctg cacaggctcg
    12241 gtatccgtac gctcgaagaa ctgcggcaaa acggttcggt caaagccttt ctgctgctca
    12301 aagcatcggg tttgaccctc acgaaaagca cgctgtggca actcgagtcg ctgctggacg
    12361 gcacgccgcc gcaagaaatg tcccaagcgc ataaagccta cctgcttgcc gagttgaaaa
    12421 accatccgcc cgtcaccgcc tttccgccgc aggaagaaat ggaacacttc atgcgcgaag
    12481 cactgcgcca agcaaaacaa tccgccgccg acggcgaaat acccgtcggg gcagtcatcg
    12541 tttcagacgg caaaatcatc gcatcggcac acaacacctg cattgccgac tgcaatgtca
    12601 gccgccacgc cgaaatcaac gccttggcac aggcaggcag cgaaatgcaa aactaccgcc
    12661 ttgacggatg cgacatatat atcaccctcg aaccctgcgc catgtgcgcg tccgcactga
    12721 tacaggcacg aatcaagcgc gtgatatacg gtgcggcaga acccaaaacc ggcgcggcag
    12781 gcagcattgt caacctgttt gccgacaaac gcctcaatac gcacaccgcc atacggggcg
    12841 gaatcctgca agaagaatgc cgcgccgtat taagccgttt ttttcagaac aaaagaaaag
    12901 gttgaaccta tgaccgtact gaccgtcatc ctcgccctcg ccctgatagc cgtcggcacg
    12961 gcaggcatcg tctatcccgc cctgcccgga ttggcattga tgtttgccgg aacatggctg
    13021 ctcgcctact ccggcggcta ccaaatctac ggcgcaggca tcctgtggac ggtcggactc
    13081 atcagccttg ccggcatact ggcggactat gtggcaggca tatgggggac aaaatatacc
    13141 ggagcaggca ggctcgccgt tcgcggcgca ttggccggca gcatcgtcgg catatttttc
    13201 tcccttcccg gccttgtact cggccccttt atcggcgcgg cggcaggcga actcatcgac
    13261 agacgcaata tgcttcaggc aggtaaagcg ggcttgggta cgctgttggg gcttatcgtc
    13321 ggtacggcgt tcaaaatcgg ctgcgccgta tccatcttgt ttatcctgtt ggtgaaatac
    13381 atcgcctacc tgttttaacc ccttcagacg gcatacggat aaattgctat aatgccgtct
    13441 gaaatttccg acaaagcacc atcatggcaa accaaagacc catctacggc ttccacgccg
    13501 tcaatgcccg tttgtggcaa aaccccaaat ccatcgtcga actctacatc caagaaggca
    13561 aatcagacgc acgcacgcgc gaagtgttgg aaaaggcggc aaacgaaaac atccgcgtat
    13621 attttgccga tgccgaccgc ctcaacgcca tcagcaaagg cgcgcgccat cagggcgtag
    13681 tcggatttat cgatgcctcc aaaaaccacg tccacctcga agacgtattg gaaaacttga
    13741 gcgaaccgcc gctgc
//
LOCUS       DAWHSK010000024        13725 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.024, whole genome shotgun sequence.
ACCESSION   DAWHSK010000024 DAWHSK010000000
VERSION     DAWHSK010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13725
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.024"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(18..1313)
                     /gene="tyrS"
                     /locus_tag="WIC03_000512"
     CDS             complement(18..1313)
                     /gene="tyrS"
                     /locus_tag="WIC03_000512"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEZ3883063.1"
                     /translation="MSVIQDLQSRGLIAQTTDIEALDALLNEQKISLYCGFDPTADSL
                     HIGHLLPVLALRRFQQAGHTPIALVGGATGMIGDPSFKAAERSLNSAETVAGWVESIR
                     SQLTPFLSFEGGNAAIMANNADWFGKMNCLDFLRDIGKHFSVNAMLNKESVKQRIDRD
                     GAGISFTEFAYSLLQGYDFAELNKRHGAVLEIGGSDQWGNITAGIDLTRRLNQKQVFG
                     LTLPLVTKSDGTKFGKTEGGAVWLNAKKTSPYQFYQFWLKVADADVYKFLKYFTFLSI
                     EEIDAVEAKDQASGSKPEAQRILAEEMTRLIHGEEALAAAQRISESLFAEDQSSLTES
                     DFEQLALDGLPAFEVSDGINVVEALVKTGLASSNKEARGFVNSKAVLLNGKPAEANNP
                     NHAAERPDDACLLTDEHKRFGKYTILRRGKRNHALLVWK"
     gene            complement(1376..3253)
                     /locus_tag="WIC03_000513"
     CDS             complement(1376..3253)
                     /locus_tag="WIC03_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEZ3883064.1"
                     /translation="MSQALPYRPDIDTLRAAAVLSVIVFHIEKNWLPGGFLGVDIFFV
                     ISGFLMTEILLREMSGGGRFSLKAFYIRRIKRILPAFFAVLAATLIGGFFLFTKDDFF
                     LLWKSALTALGFASNLYFARGKDYFAPAQEEKPLLHIWSLSVEEQFYFVFPILLLLVA
                     RKSPRVQFGFLAALCALSLAASFMPSAFDKYYLPHLRACELLIGSLTAVWMRCRQQRQ
                     PAIGRRCAAAGVLFAVCILSACLFAYSEQTAYFPGPAALIPCLAVAALIYFNHYEHPL
                     KKFFQWKITVAAGLISYSLYLWHWPILAFMRYIGPDNLPPYSPAAAIVLTLAFSLISY
                     HCIEKPFKKWQGSFAQSVLWIYALPMLILGAGSFFAMRLPFMAQYDRLGLTRSNTSCH
                     NNTGKQCLWGDTEKQPELLVLGDSHADHYKTFFDAVGKKEKWAATMVSADACAYVEGY
                     ASRVFQNWAACRAVYRYAEEHLPRYSKVVLAMRWGSQMPENSRSLAYDAGFFKKFDRM
                     LHKLSSEKQAVYLMADNLASSYNVQRAYILSSRIPGYRQTLRPDDESTLKANARIREL
                     AAKYPNVYIIDAAAYIPANFQIGGLPVYSDKDHINPYGGTELAKRFSEKQRFLDTRHN
                     H"
     gene            complement(3356..3535)
                     /locus_tag="WIC03_000514"
                     /pseudo
     CDS             complement(3356..3535)
                     /locus_tag="WIC03_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356608.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3693..4784)
                     /gene="ychF"
                     /locus_tag="WIC03_000515"
     CDS             complement(3693..4784)
                     /gene="ychF"
                     /locus_tag="WIC03_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002642527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEZ3883065.1"
                     /translation="MSLKCGIVGLPNVGKSTLFNALTQSGIEAANYPFCTIEPNVGIV
                     EVPDPRMAELAKIVNPQKMQPAIVEFVDIAGLVAGASKGEGLGNQFLANIRETDAIVN
                     VVRCFDDDNIVHVAGRVDPIADIETIGTELALADLASVEKAIVREEKRARSGDKDAQK
                     LVDLCKKLLPHLDEGKPVRSFGLDAEELTMLKPLFLLTAKPAMYVGNVAEDGFENNPH
                     LDRLKELAEKENAPVVAVCAAMESEIAELEDDEKAEFLAEMGLEEPGLNRLIRAGYDL
                     LGLQTYFTAGVKEVRAWTIHKGDTAPQAAGVIHTDFERGFIRAQVISYDDFVSLGGEA
                     KAKEAGKMRVEGKEYVVQDGDVMHFLFNV"
     gene            complement(4897..6573)
                     /locus_tag="WIC03_000516"
     CDS             complement(4897..6573)
                     /locus_tag="WIC03_000516"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEZ3883066.1"
                     /translation="MSFKTDAEIAQSSTMRPIGEIAAKLGLNADNIEPYGHYKAKINP
                     AEAFKLPQKQGRLILVTAINPTPAGEGKTTVTIGLADALRHIGKDSVIALREPSLGPV
                     FGVKGGAAGGGYAQVLPMEDINLHFTGDFHAIGAANNLLAAMLDNHIYQGNELDIDPK
                     RVLWRRVVDMNDRQLRNIIDGMGKPVDGVMRPDGFDITVASEAMAVFCLAKDIGDLKE
                     RLGNILVAYAKDGSPVYAKDLKAHGAMAALLKDAIKPNLVQTIEGTPAFVHGGPFANI
                     AHGCNSVTATRLAKHLADYAVTEAGFGADLGAEKFCDIKCRLAGLKPDAAVVVATVRA
                     LKYNGGVERANLGEENLDALAKGLPNLLKHISNLKNVFGLPVVVALNRFVSDSDAELA
                     MIEKACAEHGVEVSLTEVWGKGGAGGADLARKVVNAIESQTNNFGFAYDVELGIKDKI
                     RAIAQKVYGAEDVDFSAEASAEIASLEKLGLDKMPICMAKTQYSLSDNAKLLGCPEGF
                     RIAVRGITVSAGAGFIVALCGNMMKMPGLPKVPAAEKIDVDEHGVIHGLF"
     gene            6748..7179
                     /locus_tag="WIC03_000517"
     CDS             6748..7179
                     /locus_tag="WIC03_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEZ3883067.1"
                     /translation="MGSNAWLFWALASAGFASLTAIFAKMGLQGIDSDFATFIRTLVI
                     LAALVLFLTYTGKWQGVNGFTGKNWTFLILSGLATGASWLAYFKALQLGNASQVAPVD
                     KFSLVLVALMAVVFLDERPSTQEWIGLGLVTAGVLVLALKR"
     gene            complement(7425..8120)
                     /locus_tag="WIC03_000518"
     CDS             complement(7425..8120)
                     /locus_tag="WIC03_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEZ3883068.1"
                     /translation="MKAMILAAGRGERMRPLTDTTPKPLLDVAGKPLIGWHLCRLKQA
                     GFTEIVINHAWLGRQIEDALGDGSAYGVNIAYSPEPAGGLETAGGIAQALPLLGGQPF
                     LVVNGDVLTDIDFTAAFQAASSLPEHISAHLWLVENPPHHPDGDFLLLPDGSVRPEVN
                     GGNGLTFSGVGIYRPEMFDGIEAGSVAKLAPVLRGEMRQNRVSGQKHTGLWLDVGTVC
                     RLKEAQALAGAWK"
     gene            complement(8138..8638)
                     /gene="hpaC"
                     /locus_tag="WIC03_000519"
     CDS             complement(8138..8638)
                     /gene="hpaC"
                     /locus_tag="WIC03_000519"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     reductase component"
                     /protein_id="HEZ3883069.1"
                     /translation="MADLQKTFQTSFRDAMASCAAGVHVITTDGAAGRYGITMTAVAP
                     VTDEPPTVMLCINRSARIIPILSENGSLCINMLADEHQDVAEHFAGLTGLSPEERFAY
                     HIWHRGKTGQLEIEGALAHLHGHIVGQHEIGTHFVFYVRLDEIKNCVGKRPALLYFRR
                     QFRSLD"
     gene            complement(8753..9193)
                     /gene="nadR"
                     /locus_tag="WIC03_000520"
     CDS             complement(8753..9193)
                     /gene="nadR"
                     /locus_tag="WIC03_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family adhesin repressor NadR"
                     /protein_id="HEZ3883070.1"
                     /translation="MPTQSKHASINISLIQAREALMTQFRPILNQANITDQQWRIIRL
                     LAENGTLDFQDLANQACILRPSLTGILTRLEKAGLVVRLKPSNDQRRVFLKLTAEGEK
                     LYEEIGEEVDERYDAIEEVLGREKMLLLKDLLAELAKIEDALNS"
     gene            complement(9536..10045)
                     /locus_tag="WIC03_000521"
     CDS             complement(9536..10045)
                     /locus_tag="WIC03_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein disulfide oxidoreductase"
                     /protein_id="HEZ3883071.1"
                     /translation="MKKKLLSGIKFAVQTALVFLLVSLFLDWVRKPEEPAGAAGRPLT
                     LLSGQRLTLGQFSRDRTVLVYFWGSWCGVCRYQSPIIDDLAADGVPVVGVAVRSGSAS
                     EVAAYMAKRGLGFPTVNDEDGGLARSWRIVATPAVVLVKNGKMVHYTTGISSYWGLRA
                     RIFQADVFG"
     gene            complement(10124..10199)
                     /locus_tag="WIC03_000522"
     tRNA            complement(10124..10199)
                     /locus_tag="WIC03_000522"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10164..10166),aa:Lys,seq:ttt)
     gene            10410..11489
                     /gene="apbC"
                     /locus_tag="WIC03_000523"
     CDS             10410..11489
                     /gene="apbC"
                     /locus_tag="WIC03_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HEZ3883072.1"
                     /translation="MNIQNIRTLLDTVAVPNTARTLGSEKAVRSVEQRSDGIHIALHF
                     GFPVAHIASETADRIQEILMPLTGDTHIHLSMDTEIGTHKVQPGVTTIKGVKNIIAVA
                     SGKGGVGKSTTTANLAAAMARMGARVGVLDADLYGPSQPTMLGVHDRKPDQKNQKLIP
                     VESSDGIQVMSIGFLVDTGQAVVWRGPMVSQALQQLMFQSEWDEVDYLFIDLPPGTGD
                     IQLTLSQRIPVTGSVIVTTPQDIALIDARKAVDMFRKVNIPILGVLENMSVHICSNCG
                     HSEALFGTDGGKDLAARLNVPLLGQLPLSLPVREAMDGGTPAQLFDEHPAIARIYTDA
                     AFQIALSIADKGKDFSSRFPKIVVE"
     gene            12291..>13725
                     /locus_tag="WIC03_000524"
     CDS             12291..>13725
                     /locus_tag="WIC03_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly/adherence protein PilC"
                     /protein_id="HEZ3883073.1"
                     /translation="MNKTLKRQVFRHTALYAAILMFSHTGGGGAQAQTSKYAIIMNEG
                     NQLEVKQNGQYSTIKDKDREREYTHHPSRGGGGGSVSFNNSDELVSRQNGTAVFGTAT
                     YLPPYGKVSDFDADGLKERNNAAGWIRTTRIALAGYSYANVVCRSNAGCPKLVYKTQF
                     SFDNPDLAKIGGGLDRHTEPSRDNSPIYKLKDHPWLGVSFNLSAKGTANDGKTINKFD
                     EKNSNNNLVYTTESGDISLGNRDRETTAMAYYLNAKLHLLDKKQIQNITNKTVQLGVL
                     RPSIDVRQGNTGISGLLTFWARWDIKDTGQIPVKLSLTQVKAGRCINKSNPNPKSKAP
                     SPALTAPALWFGPVKDGKAEMYSASVSTYPDSSSSRIFLQNLKRKTDHSKPGRHSLAT
                     LSENEIKSKEPSFTSRQTIIRLDGGVQQIKLDKSNEATGLNGNTNNNTFGIVKDLGVE
                     PDTSEWKKVLLPWTVRASNNDNQFKTFN"
BASE COUNT         3257 a         3749 c         3685 g         3034 t
ORIGIN      
        1 tttctgcggc aatcggatta tttccaaacc aaaagcgcgt gattgcgttt gccgcgccga
       61 aggatggtgt atttgccgaa acgtttgtgt tcgtcggtca gcaggcaggc atcgtcgggg
      121 cgttcggcgg cgtggttggg gttgttggct tcggcaggtt tgccgttgag caaaaccgct
      181 ttgctgttca caaagccgcg cgcttcttta ttggaggatg ccaaaccggt tttgaccaag
      241 gcttcgacga cattgatgcc gtctgaaact tcaaatgcag gcaggccgtc gagggcgagc
      301 tgctcgaagt cgctttcggt caggctgctt tggtcttcgg caaacaggct ttcggaaatg
      361 cgctgcgcgg cggcaagggc ttcttcgccg tgaatcaggc gggtcatttc ttcggcgagg
      421 atgcgttgcg cttcgggctt gctgccgctt gcctggtctt tggcttcgac ggcatcgatt
      481 tcttcgatgg acaggaaggt aaagtatttc aggaatttat acacatcggc atcggcgact
      541 ttcagccaga attggtagaa ctgatagggc gaggtttttt tcgcgttcag ccatactgcg
      601 ccgccttcgg ttttgccgaa tttggtgccg tctgatttgg taaccaaagg cagggtcagg
      661 ccgaacactt gtttttgatt caggcggcgg gtcaggtcga taccggcggt gatgttgccc
      721 cattggtcgg agccgccgat ttccaaaacc gcgccgtggc gtttgttcaa ctcggcgaag
      781 tcgtagcctt gcagcaggga gtaggcgaac tcggtgaagg aaatgcctgc gccgtcgcgg
      841 tcaatgcgct gtttgacgga ttctttgttc agcatggcgt tgacggagaa atgcttgccg
      901 atgtcgcgca ggaagtcgag gcagttcatt ttgccgaacc agtcggcgtt gttcgccata
      961 atggcggcgt ttccgccttc aaagctcaag aaaggggtta attggctgcg gatgctttcc
     1021 acccagccgg caacggtttc ggcggaattc aagctgcgtt cggcggcttt gaagctgggg
     1081 tcgccgatca taccggtcgc accgcccacc agtgcaatcg gcgtatgccc cgcctgttgg
     1141 aagcggcgca atgccaatac gggcagcagg tgtccgatgt gcaggctgtc ggcggtcggg
     1201 tcgaagccgc aataaaggga aattttttgt tcgttcaaca aagcgtctaa ggcttcgatg
     1261 tcggtggttt gcgcgataag gccgcgcgat tgcaggtctt ggatgacgct catcggtctc
     1321 tttcaaaaaa aattagcggt tttgcaaacc gccgattgta acaaatttaa gcgaatcaat
     1381 ggttatggcg cgtatcgaga aaccgttgtt tttcggaaaa acgcttcgcc aattccgtgc
     1441 cgccgtaagg gttgatgtgg tctttgtccg agtaaaccgg caatccgccg atttggaaat
     1501 ttgcggggat ataggcggcg gcatcaataa tatagacgtt ggggtatttg gctgccaatt
     1561 ccctgatgcg tgcattggct ttcagagtgc tttcgtcgtc cgggcgcagg gtttggcggt
     1621 aacccggtat gcgtgaagac aagatatagg cgcgctggac gttgtaagac gaggcaaggt
     1681 tgtccgccat caggtaaacg gcttgttttt cggacgagag tttatgcagc atacggtcga
     1741 attttttgaa aaaaccggca tcgtaggcaa gggagcggct gttttcgggc atttggctgc
     1801 cccaccgcat cgccaaaacc acttttgaat accggggcag gtgttcttcg gcatagcggt
     1861 aaacggcgcg gcaggcggcc cagttttgga atacgcggga cgcgtagcct tccacatagg
     1921 cgcaggcgtc ggcggaaacc atagtggcag cccatttttc ttttttgccc acggcatcga
     1981 agaatgtttt gtaatggtcg gcgtgggagt cgcccaaaac cagcagttcc ggctgttttt
     2041 ccgtatcccc ccataggcat tgtttgccgg tattgttgtg gcaggaggtg ttggaacgcg
     2101 tcagccccaa gcggtcgtat tgcgccataa acggcagcct catcgcaaaa aatgagcccg
     2161 cccccaaaat gagcataggc aaggcataaa tccataaaac ggattgtgcg aacgaacctt
     2221 gccatttttt aaacggcttt tcgatgcagt gataagaaat cagggaaaac gccagagtca
     2281 ggacgattgc cgccgccggc gaataaggcg gcaggttgtc cgggccgata tagcgcataa
     2341 aggccaatat cggccaatgc cacagataaa gcgaatagga aatcaaaccg gcggcaacgg
     2401 tgattttcca ttggaaaaat tttttaagcg ggtgttcgta atggttgaaa taaattagcg
     2461 cggcaacagc cagacaggga atcaaagcgg cgggccccgg gaaataggcg gtttgttccg
     2521 aataggcaaa caggcaggcg gacagtatgc acacggcaaa caatacgccg gcggcggcac
     2581 agcgtctgcc gatagcgggt tgccgctgtt gccggcagcg catccacacg gcggtcagcg
     2641 atcctatcag taattcgcag gcgcgcaggt ggggcaggta atatttatcg aacgcggaag
     2701 gcataaagga agcggcaagg cttaaggcgc acagtgcggc gaggaagccg aactgtacgc
     2761 gcgggctttt gcgggcgaca agcagcagca atatcggaaa gacaaagtaa aattgttctt
     2821 cgaccgacaa agaccaaatg tgcagcaggg gcttttcttc ctgcgcggga gcgaaatagt
     2881 ccttgcccct tgcaaaatac aggttggatg cgaaacccaa ggcggtcagc gcggatttcc
     2941 ataaaagaaa gaaatcatct ttggtgaata aaaagaagcc gcctatcagc gttgccgcca
     3001 atacggcgaa aaatgcgggc agaatccgct tgatgcggcg gatataaaat gccttcaggg
     3061 aaaacctccc cccccccgac atttcgcgaa ggaggatctc cgtcatcaaa aagcctgaaa
     3121 tcacaaagaa tatatcgaca ccgagaaatc cgcccggcag ccaatttttt tcgatatgga
     3181 acacgatgac ggacaaaacg gcggcggcgc gcaatgtgtc gatgtccggg cggtagggta
     3241 aggcttggct cataatgttt ttatagtgga ttaacaaaaa ccagtacggc gttgcctcgc
     3301 cttgccgtac tatctgtact gtctgcggtt tcgtcgcctt gtcctgattt aaagttaatc
     3361 cactatactc gaaacgcggc ggcgcaaatg ccgtctgaaa ggtcattttg tatcggggct
     3421 cgggatattc ggaatgccgg acggcttttt gcaacagcgg ggcaggcggt ttgttttgca
     3481 ggaatcgggg agggcaaatc ggaaatgcgg gtgggatttt atagtggatt aacaaaaacc
     3541 ggtacagcgt tgccccgcct tgtcctgatt tttgttaatc cgctatattt tgatacggct
     3601 gcattccggc ggtatggaaa cgccgaaaac caccaaaacc ggcttcagac ggcatttctg
     3661 gcaaagccgt ctgaaaacct gccgcatttg ggttacacgt taaacaaaaa gtgcatcaca
     3721 tccccgtcct gcacgacata ttccttgcct tccacgcgca ttttgccggc ttctttggct
     3781 ttggcttcgc cgccgagtga gacaaaatcg tcgtaggaaa tgacttgggc gcggatgaag
     3841 ccgcgttcga agtcggtgtg gatgacgccg gcggcttgcg gggcggtgtc gcctttgtgg
     3901 atggtccagg cgcggacttc tttgacgccg gcggtgaagt aggtttgcag gcccaagagg
     3961 tcgtagcccg cgcggatcag gcggttcagg ccgggttctt ccaagcccat ttcggcgagg
     4021 aactcggctt tttcgtcgtc ttccaactcg gcgatttcgc tctccatcgc ggcgcaaacg
     4081 gcaacgacgg gggcgttttc tttttcagcc aattctttca ggcggtcgag gtgcggattg
     4141 ttttcaaaac cgtcttcagc aacgttgccg acatacatgg cgggtttggc ggtgagcagg
     4201 aacagcggtt tgagcatggt gagttcttcc gcgtctaagc cgaaagaacg gacgggtttg
     4261 ccttcgtcca gatgcggcag cagttttttg cacaaatcga ccagcttttg cgcgtctttg
     4321 tcgcctgaac gggcgcgttt ttcttcgcgg acgatggctt tttccacact tgccaagtcg
     4381 gcaagcgcca actctgtacc gatggtttcg atgtcggcaa tcggatcgac gcggcctgca
     4441 acgtggacga tgttgtcgtc gtcaaagcag cgtacgacgt taacaatcgc gtcagtctcg
     4501 cggatgttgg caaggaactg gttgcccaag ccctcgcctt tgctcgcgcc tgcaaccaaa
     4561 ccggcaatat cgacaaattc gacgatggca ggctgcattt tttgcggatt gacgattttt
     4621 gccagctccg ccatacgcgg gtcggggact tcgacgatgc cgacgttggg ttcgatggta
     4681 cagaaaggat agtttgccgc ttcgataccc gattgggtca gcgcgttaaa aagggtggat
     4741 ttgccgacgt tgggcaaacc gacgatgccg catttcaaac tcatgttttt tcctgaaaat
     4801 agagaaattt aacggcggat tatagcatac cgccgcccgc gttccgaaaa atgccgtctg
     4861 aaacggcttc agacggcatc cggtttcaaa aaccgttcag aacaagccgt gaatcacgcc
     4921 gtgttcgtcc acatcgattt tctcggcggc cggaactttc ggcagacccg gcatcttcat
     4981 catattgccg cacagcgcga cgatgaagcc cgcgccggcg gaaacggtga tgccgcgcac
     5041 ggcgatgcgg aagccttcgg ggcagcccaa cagtttggcg ttgtcactca aagagtattg
     5101 ggttttcgcc atgcagatcg gcattttgtc caagcccagt ttttccagtg aagcgatttc
     5161 ggcagacgct tccgcgctga aatcaacatc ttccgcgccg tacacttttt gggcaatcgc
     5221 acggattttg tctttgatgc ccaactcgac atcgtaggcg aaaccgaagt tattggtttg
     5281 actttcaatg gcgttgacga ctttgcgcgc caaatccgcg ccgcccgcac cacctttgcc
     5341 ccacacttcg gtcagggaaa cttcaacgcc gtgttcggca caggcttttt caatcatcgc
     5401 caactcggca tcggagtcgg acacgaagcg gttgagcgca acgacgacgg gcaggccgaa
     5461 tacgtttttc aggttggaaa tgtgtttcag caggttgggc aggccttttg ccaaagcgtc
     5521 gaggttttct tcgccgaggt tggcacgttc cacgccgccg ttgtatttca aagcgcggac
     5581 ggtggcgacg acgacggctg cgtcgggttt caaaccggca aggcggcatt tgatgtcgca
     5641 gaatttttcc gcgcccaaat ccgcgccgaa gcctgcttcg gttacggcgt aatcggcaag
     5701 gtgtttcgcc agacgggttg cggttacgga gttgcagccg tgggcgatgt tggcgaacgg
     5761 gccgccgtgt acgaaggcgg gcgtgccttc gatggtttgc accaagttag gcttaatcgc
     5821 atctttgagc agggccgcca tcgcgccgtg tgccttcaaa tctttggcgt aaacggggct
     5881 gccgtctttg gcgtaggcga caaggatgtt gcccaaacgc tctttcaaat cgccgatgtc
     5941 tttggcgaga cagaataccg ccatcgcttc ggaggcaacg gtaatgtcga aaccgtcggg
     6001 gcgcatcacg ccgtcaacag gtttgcccat tccgtcgatg atgttgcgca actggcggtc
     6061 gttcatatcg accacgcgcc gccacagcac gcgtttgggg tcgatgtcca actcgttgcc
     6121 ctgatagata tggttgtcga gcatcgcggc aagcagatta tttgccgcac cgatggcatg
     6181 gaaatcgccg gtgaagtgca ggttgatgtc ttccatcggc aaaacttggg catagccgcc
     6241 gcctgccgca ccgcctttaa caccgaacac cggccccaaa gaaggttcgc gtagggcgat
     6301 aaccgagtct ttgccgatat ggcgcaacgc atccgccaaa ccgatggtta ctgtggtttt
     6361 accctcaccc gcaggagtcg gattaatggc agtaaccaaa atcagacggc cttgtttttg
     6421 cggcagcttg aatgcttcgg caggattgat tttggctttg tagtgaccgt aaggctcaat
     6481 gttgtcggcg ttcaggccca gcttggcggc aatttcgcca atcgggcgca tggtggaaga
     6541 ctgggcgatt tcggcatcgg ttttgaagct catgattttc ctttagaaat gaggagggac
     6601 ataccgtctg aaagcatcag gcgacaaaca ggtggattga aaataatatc aggcatatta
     6661 taacgttatc cgcaccaaac ccgcagtgaa atttttgacg ctgcaacaaa aataccgttc
     6721 atattgttca caatccaagg agaaaacatg ggcagcaacg catggctgtt ttgggcattg
     6781 gcatcggcag gcttcgcctc gctgaccgcc attttcgcca aaatgggttt gcagggcatc
     6841 gattcggatt tcgccacctt tatccgcacc ttggtgatcc tcgccgcttt ggtactgttt
     6901 ttgacctaca ccggcaaatg gcagggcgtg aacggattta caggaaaaaa ctggacattc
     6961 ttaattctgt cgggtttggc gaccggcgca tcctggctcg cctacttcaa agccctgcaa
     7021 ctgggtaacg cctcgcaagt cgcccccgtc gacaaattca gcctggtctt ggtcgcgctg
     7081 atggcggtgg ttttcttgga cgaacgcccc agcacgcagg aatggatagg cttggggctg
     7141 gtaacggcgg gcgtgttggt gctggcgttg aaacgttaaa ccgaatccgc cataccgcct
     7201 gaaaccgggt tttgacaatc tgacgggcgg cggtctgaac aaagttaccc aataaaatcc
     7261 gacaccgcgc cgacggcaaa ccgccgcatc cgacaggggt cggcttcaga tggcatttgt
     7321 tggacggaaa gccccgaacg ccctattttc acgcctccac gaaaatatgc cggacatccg
     7381 gtcggaaccg ttcggcagcc ggctcggcaa gccaccgttt tatttcactt ccaagcccct
     7441 gcaagggctt gagcctcttt cagacggcat accgtgccga catccagcca caagcccgta
     7501 tgcttctgac cgctcacgcg gttttgccgc atttcgccac gcaatacggg cgcgagtttc
     7561 gccacactgc ccgcttcgat tccgtcaaac atttcaggac ggtaaatacc cacgccgctg
     7621 aatgtcaatc cgttgccgcc atttacttcc ggccgcacgc tgccgtccgg caacagcaaa
     7681 aaatcgccgt caggatggtg cggcggattt tccaccagcc acagatgggc ggaaatatgt
     7741 tccggcaggg acgatgccgc ctgaaacgcg gcggtaaaat cgatgtcggt cagcacgtcg
     7801 ccgttgacca ccaaaaacgg ctgcccaccc aacagcggca atgcctgcgc gatgccgcct
     7861 gccgtttcca aaccgcctgc gggttcgggc gaataggcga tgttcacgcc ataagccgag
     7921 ccgtcgccca aagcatcttc tatctgccgg cccagccaag cgtggttgat gacgatttcg
     7981 gtaaaccccg cctgcttcag acggcatagg tgccaaccga ttagaggctt acccgccaca
     8041 tcgagcagcg gcttcggagt ggtatcggtc aaaggacgca tacgctcgcc gcgtcctgcc
     8101 gccagtatca tcgctttcat atatatctgt ccgaatatca gtctaaagat ctaaactgcc
     8161 gtctgaaata aagcagcgcg ggacgtttgc ccacgcaatt tttaatttcg tcgagcctga
     8221 cgtaaaacac aaaatgtgtg ccgatttcat gttggccgac aatatgcccg tgcagatgcg
     8281 ccaacgcgcc ttctatttca agttgtcccg ttttgccgcg atgccaaata tggtaggcaa
     8341 accgctcttc gggcgacagg ccggtcagcc cggcaaaatg ttcggcaaca tcctgatgtt
     8401 cgtccgccag catattgatg cagaggctgc cgttttccga caggatcgga atgattcgcg
     8461 cactccggtt gatgcacagc atcacggtcg gcggctcgtc ggtaaccggc gcgaccgccg
     8521 tcattgtaat gccgtaacgc cctgccgcac cgtctgtcgt gatgacatga acgcctgccg
     8581 cacaggatgc catcgcatca cggaacgaag tttgaaaagt tttctgcaaa tccgccattt
     8641 ttccccttta aactgtcccc tatataagaa tgctgcacac aaggcacccc ccatgtgcag
     8701 cagttctgat tcaaaaagcc gtcggtcgga catttccgcg cgttacggcg tattacgagt
     8761 tcaacgcatc ctcgattttg gcaagttctg ccaacaggtc tttaagcagc agcattttct
     8821 cgcggcccag cacttcctcg atagcgtcgt aacgctcgtc cacttcttcg ccgatttcct
     8881 catacagctt ctcgccctcg gcagtcagct tcagaaaaac gcggcgttgg tcgttggaag
     8941 gtttcaggcg gacgaccaaa cccgcttttt caaggcgggt caggataccg gtcaggctgg
     9001 ggcgcagaat acacgcctga ttcgccaaat cttgaaagtc cagcgtgccg ttttccgcca
     9061 aaagacggat aatccgccat tgctgatcgg taatattcgc ctgattcaga ataggcctga
     9121 attgggtcat cagggcttcc cttgcctgta tcagactgat attgatagac gcatgttttg
     9181 attgggtagg cattgttcaa gtctccaagt tatcgaaaat caaactttca aaccgtcggg
     9241 aaagcctgtg ggcgtaaatt ttgatgcaac cgttatataa caaaacgaac atatagcaac
     9301 aatacgctat aaaccgcatc ggacgactgg gtataaaaga ctttaattcc gataatccta
     9361 tctaaaaata ttttaatagt tatatcttag tctattttcc ctacaatctc aacaaagtat
     9421 tacatttact gtcgaaagaa ctttcttcca gaaaaaacaa aagccgccgc atcagacgag
     9481 caaggtatat gccgtttgat tctctacata gcgtaaagtt taacaaaaac aaaagttaac
     9541 cgaaaacatc cgcctgaaaa attcgtgcgc gcaagcccca ataactgctg attcccgtcg
     9601 tatagtgaac cattttccca tttttgacca aaacgacggc aggcgttgcg acaatccgcc
     9661 aagaccttgc caaacccccg tcctcatcgt tgaccgtcgg aaagcccaag ccccgctttg
     9721 ccatatacgc cgccacttcg gacgcgctgc cggaacgtac cgccacgccg acgaccggca
     9781 cgccgtccgc cgccaaatca tcgattatcg gcgactgata acggcacacg ccgcaccagc
     9841 ttccccaaaa atacaccagc accgtcctat cccggctaaa ctgtcccaaa gtcagccgct
     9901 gccccgacag cagggtcaaa ggccgccctg ccgcaccggc cggctcttcg ggcttgcgta
     9961 cccaatccaa aaacagcgac accaataaaa acaccaatgc cgtctgaacg gcaaatttga
    10021 tgcccgaaag cagtttcttt ttcatacgcc ccctcaaacg gtacgcccgc gcaaccggca
    10081 ggcaaacaaa aagccaagtc ttaaaacttg gcttccggtt atctggtggg tcgtgagcga
    10141 ttcgaacgct cgaccaacgg attaaaagtc cgctgctcta ccggctgagc taacgacccg
    10201 ataagccgtg cattatacag caccatcctg cctcgtcaag caaattttac aggcttaatt
    10261 gcagaccact gtttgcacgg gatattttgg caacagattt tcacaatccg ccgcataccg
    10321 tgtaaaagtt cgcacaagga aaagcaaacc gcccgaaatc aatgtacact ttccgcccgt
    10381 ttcccaaact gcacacagaa acacacatta tgaacataca aaacatccgc accctcctcg
    10441 acaccgtcgc cgttccgaat acggcacgca cgctcggcag cgaaaaggcc gtccgttcgg
    10501 tcgaacagcg ttcagacggc atccatatcg ccctgcattt cggcttcccc gtcgcgcaca
    10561 ttgcctcaga aacagccgac cgcatacagg aaatcctgat gccgctgaca ggcgacacac
    10621 acatccatct gtccatggac actgaaatcg gcacacacaa agtccagccc ggcgttacca
    10681 ccatcaaagg cgtgaaaaac atcatcgccg tcgcatcggg aaaaggcggc gtgggcaaat
    10741 caaccaccac tgccaacctt gccgccgcaa tggcgcgcat gggcgcgcgc gtcggcgtgc
    10801 tcgatgccga cctttacggt ccgagccagc ccacgatgct gggcgtacac gaccgcaaac
    10861 ccgatcagaa aaaccaaaaa ctcattcccg tcgaatcttc agacggcata caggttatgt
    10921 ccatcggctt tctcgtcgat accggccaag ccgtcgtctg gcgcgggccg atggtcagcc
    10981 aagccttgca gcagctgatg ttccaaagcg agtgggacga agtggactat cttttcatcg
    11041 acctgccccc gggtacgggc gacatccagc tcacactgtc ccaacgcatc cccgtaaccg
    11101 gatcggtcat cgtaaccacg ccgcaggaca tcgccctgat agacgcgcgc aaagccgtgg
    11161 atatgttccg caaggtcaac attcccattt taggtgtatt ggaaaacatg tccgtccaca
    11221 tctgctccaa ctgcgggcat agcgaagcac tgttcggcac ggacggcggc aaagatttag
    11281 ccgcacgcct caacgtcccc ctgctcggac agcttcccct aagcctgccc gtgcgcgaag
    11341 ccatggacgg cggcacaccg gcgcaactgt tcgacgaaca ccccgccatc gcccgaatct
    11401 acaccgatgc cgcattccaa atcgccctga gcattgccga caaaggcaaa gacttcagca
    11461 gccgcttccc caaaatcgtc gtcgaataaa gccccgcccc cgaaaccgca atgccgtctg
    11521 aaaccccgcg ccgccacaac ggcggcacac aggcggacgc gcaggatagg cgcagggtat
    11581 ttcgcaggtc aggcgcagcg taggtgcagg atgagcgcag ggtgcgccgt agtgtgggct
    11641 tcagcccacc gaatccacca acccccgccg ccgtcattcc cgcgcaggtg ggaatccagg
    11701 tctgtcggcg cagaaactta tcggataaaa cggtttcttg agattttacg ttccggattc
    11761 ccactttcgt gggaatgacg gaatgtaggt ttgtgggaat gacggtttag gtatttttat
    11821 agcaagccgt agggtgggat gccgccggag ggcatcatat tcttggaata gcgcattaaa
    11881 aagagtatga taatggaaac acaaaagatt ccggcaatgc ccgtgcgccg ttcccgtccg
    11941 tcattcccgc gcaggcggga atccagacct gtcggaacgg aaacttatcg gataaacggt
    12001 ttctctagat tccgcatcct agattcccgc ctgcgcggga atgacggttc gggtattttt
    12061 ataaggcaga ttcgcaaggt gcgccataga gtgggctaaa gcccgcccta cagcccgccc
    12121 tgcgaaaccc acgaaacacc ctgcgaaaca tatgttccaa cacacagggc ggcacataag
    12181 gcgccgccct gattcggaag ggcttgcacc cctcccggac aaagcctgat cctgccgccc
    12241 cgaaggacgg atgcccgaag ggcggggggt ttgaccgaaa aggaaatacg atgaataaaa
    12301 ctttgaaaag gcaggttttc cgccataccg cgctttatgc cgccatcttg atgttttccc
    12361 ataccggcgg ggggggggcg caggcgcaaa ccagtaaata cgctattatc atgaacgagg
    12421 gaaaccagct cgaggtaaag cagaatgggc aatattcaac aataaaggac aaagacaggg
    12481 agcgcgaata tactcatcat ccgtccagag gaggaggagg aggctctgtc tcattcaaca
    12541 atagcgatga gcttgtttct cgacaaaacg gtactgccgt ttttggcaca gccacctacc
    12601 tgccgcccta cggcaaggtt tccgattttg atgccgacgg gctgaaagag cgcaacaatg
    12661 ccgccggttg gattcgtacc acccgcatcg cgctggcagg ctacagctac gccaatgtcg
    12721 tatgcagaag caacgcaggc tgtcccaaac ttgtctataa aacccaattt tccttcgata
    12781 atcccgactt ggcaaaaata ggaggcgggc tggataggca cacagagcca agccgcgaca
    12841 attcgcccat ttacaaattg aaggatcatc catggttggg cgtgtctttc aatttgagcg
    12901 ccaagggtac cgccaacgat ggtaagacaa tcaacaaatt tgatgaaaag aatagtaata
    12961 acaacctcgt ctataccacg gaaagcggcg atatttcctt gggcaacagg gatcgcgaaa
    13021 cgaccgccat ggcctattat ctgaacgcca agctgcacct gctggataaa aaacagattc
    13081 aaaatatcac caacaaaaca gtgcagttgg gtgtcttgag gccgagcatc gatgtgcggc
    13141 aaggaaatac ggggatttcc ggcttgctaa ctttttgggc taggtgggac attaaagata
    13201 ccgggcagat tccagtcaag ctcagcttga cgcaagtcaa agcaggtcgc tgcatcaata
    13261 aatcgaaccc caatcccaaa tccaaagccc cttcgcccgc actgactgcc cccgcgctgt
    13321 ggttcggacc tgtgaaagat ggtaaggcgg agatgtattc cgcttcggtt tctacctacc
    13381 ccgacagttc gagcagccgc attttccttc aaaatctgaa aagaaaaacc gaccacagca
    13441 aacccggccg ccattccctc gcaaccttga gtgagaatga gattaaaagt aaagagccga
    13501 gtttcacaag tcggcaaaca atcatccgat tggatggcgg cgtacagcag atcaaactgg
    13561 ataaaagcaa tgaggccacc ggtttaaatg gaaataccaa caacaacact ttcggcattg
    13621 ttaaggactt gggcgtcgaa cctgatacca gcgagtggaa aaaagtattg ctgccttgga
    13681 cggttcgtgc ttccaataat gacaatcaat ttaaaacatt taacc
//
LOCUS       DAWHSK010000025        13457 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.025, whole genome shotgun sequence.
ACCESSION   DAWHSK010000025 DAWHSK010000000
VERSION     DAWHSK010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13457
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.025"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(662..1429)
                     /locus_tag="WIC03_000525"
     CDS             complement(662..1429)
                     /locus_tag="WIC03_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEZ3883074.1"
                     /translation="MQTRILSAVLLAFSTAAFAGGAFTLQFDNPSEDGGFTQNQLLSA
                     PYGFGCSGGNASPALSWKNPPAGTKSFVLTVYDKDAPTGLGWMHRVVADIPADVHRRN
                     ATSLQLSRCANIADRTGLDALGGRRHSRRCPPPQRGLAAIKPLRQHRRRPVRSHIGGN
                     QFADLPHQVDAFVHGKTDAVMLQPRQHAAKRDLRRIVRHFFIRQHRRRIIKRRAHPPG
                     VRQLHRIAHCLRRPVLCGEPFYGFLFESNPLRLIEAV"
     gene            1740..2492
                     /locus_tag="WIC03_000526"
     CDS             1740..2492
                     /locus_tag="WIC03_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEZ3883075.1"
                     /translation="MITLPSLNNLPSKWDEIRYWLETRVCECGVMPHPNLTECEAGQF
                     DRDFWDNIGCAPEEYVRIRRAIRLLEARYPDSLNELVCAAIATPLGEMLAVFGNKGLC
                     LLEFVGQKHLEQEIAAVQKALRGRFVFREDERTQLLRQELDLYFKGRLKTFATPLEQI
                     GTEFQKQAWDALLAIPYGETRSYKEQAQRLGNPNAVRAVAAANGQNKVSILIPCHRVI
                     GSDGKLTGYAGGLNRKQFLLALERGEVQTALF"
     gene            2683..2758
                     /locus_tag="WIC03_000527"
     tRNA            2683..2758
                     /locus_tag="WIC03_000527"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2716..2718,aa:Asn,seq:gtt)
     gene            2889..3899
                     /gene="waaF"
                     /locus_tag="WIC03_000528"
     CDS             2889..3899
                     /gene="waaF"
                     /locus_tag="WIC03_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HEZ3883076.1"
                     /translation="MSIKILIISPSWIGDCVMTQPLFRRLKELHPGCTIDVFAPKWSM
                     AVFERMPEVNEILENPFGHGALELKRRWRVGRDLGRRGYDQVIVLPGSLKSAIIALAT
                     GIGKRTGYVGESRYFLLNDIRRLDKERLPLMVDRYTALAHQSQEDFDGCSGFPEFSID
                     ERRREISVETFGLNLGKPVLAFCPGAEFGPAKRWPTRHFAELGKHYSAAGWQVWLFGS
                     QKDDEIAEEINRLSDGMCVNLCGKTDLSQAMDLLSLADTVVCNDSGLMHLAAALGRKV
                     VAVYGSSSPTHTPPLSDRAKIVSLHLECSPCFKRECPLGHTDCLNRLYPEKIVQAVEE
                     AV"
     gene            3954..4400
                     /gene="smpB"
                     /locus_tag="WIC03_000529"
     CDS             3954..4400
                     /gene="smpB"
                     /locus_tag="WIC03_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEZ3883077.1"
                     /translation="MAIANNKKAFHDFFIEDRIEAGLVLEGWEVKAIRAARVQLKESY
                     IYWKKDAFYLVGCHITALPTASTHIKPDAVRPRKLLLNQSEINKLIGKTERAGYTIVP
                     LDLHFSRGKIKMEIGLAKGKKQHDKRQSMKEADWKREKQRLIKHTR"
     gene            4766..6805
                     /gene="autB"
                     /locus_tag="WIC03_000530"
     CDS             4766..6805
                     /gene="autB"
                     /locus_tag="WIC03_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_180374913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phase-variable autotransporter AutB"
                     /protein_id="HEZ3883078.1"
                     /translation="MYYETRSKQASKQKFKKSFIISLFFSILYTSPLLAVDYVYDKTK
                     LTDDEITRLKKLRDRNSEYWKEETYLITGDNPKVPSVPALYPRTYQFENINNSKKISF
                     YDQKYTEDYLVGFARGLGVAKRNGDTEEQIRKYFKECFNSNTKIRDYSTCQAEKFGFH
                     PLIVKSHIFSLGPKIKNSHINSEILSVGNYTEWANQVIHHIENYVSFAAHLYSGLDPF
                     HYIEVTDNSHVIGQTISLDEFRLENSLWEPRWDSNVGKLKTTNADIRFNTKSESLLVK
                     EDYAGGARFRFAYDPKEAKNTALIFEKNVTGTSDIIFENPIDDLKSLDGHQIIKVNGT
                     ADKHAFRLSGKHQKGIYTLSLQQRPEGFFTKVQERDDIAIYAQQAQAANTLFNLRLNN
                     KNNDIFDRTLPRKGLWLRVIDGHSNQWVQGKTAPVESYRKGVQLGGEVFTWQNESNQL
                     SIGLMGGQAEQRSTFRNPDTDNLTTGNVKGFGAGVYATWHQLQDKQTGAYADSWVQYQ
                     RFRHSINTEDATERFTSKGITASIEAGYNALLAEHFTEKGNRVRFYLQPQAQLTYLGV
                     NGKFSDSENAQVNLLGFRQLQSRVGVQAKAQFAFTNGVTFQPFVAVNSIYQQKPFGLE
                     MDGERRVINNKTAIESQLGVAVKIKSHLTLQATFNRQTGKHHQAKQGALNLQWTF"
     gene            complement(6985..8352)
                     /locus_tag="WIC03_000531"
     CDS             complement(6985..8352)
                     /locus_tag="WIC03_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEZ3883079.1"
                     /translation="MPDLHTEFSRLLPADEIAELSPTLLKDQRNRFTSAPDIILQPRS
                     VESVQTIMRFCYEHRIPVTPQGGNTGLCGAAVSENGVLLNLSKLNRIRSINLSDNCIT
                     VEAGSVLQTVQQAAEASNRLFPLSLASEGSCQIGGNIACNAGGLNVLRYGTMRDLVIG
                     LEVVLPNGELVSHLHPLHKNTTGYDLRHLFIGSEGTLGIITAATLKLFARPSDKATAW
                     VGIPDIESAVRLLTETQAHFAERLCSFELIGRFAAELSSEFSKFPLPTHSEWHILLEL
                     TDSLPDSNLDDRLVEFLYKKGFTDSVLAQSEQERIHMWALRENISASQRKLGTSIKHD
                     IAIPIGRVADFVRRCAKDLEQNFKGIQIVCFGHLGDGSLHYNTFLPEILSNEVYRYEN
                     DINDAVYRNVLACNGTIAAEHGIGIIKKQWLDKVRTPAEIALMKSIKQHLDPYNIMNP
                     GKLLP"
     gene            complement(8462..8773)
                     /locus_tag="WIC03_000532"
     CDS             complement(8462..8773)
                     /locus_tag="WIC03_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883080.1"
                     /translation="MKKSLFAAALLSLVLAACGGEKAAEAPATEAPAAEAPATEAPAA
                     EAPATEAPAAEAPATEAPAAEAPAAEAAATEAPAAEAAATEAPAAEAPAAEAPAAEAA
                     K"
     gene            9246..9728
                     /locus_tag="WIC03_000533"
     CDS             9246..9728
                     /locus_tag="WIC03_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEZ3883081.1"
                     /translation="MAIVKNSVVSLHYEMYDANNQLLDKTEEPIAYLHGGYDGIFPLV
                     EEALHGKDAGDTVDVALSPDDAFGEQDPELVRIEDAGVFPVEVEVGMMFEADDPETGD
                     VVVYRVTDVADGKAVVDGNHPLAGMKIRFKATVESVRDASDEEIAHGHVHGPHGHHHH
                     "
     gene            9815..10627
                     /gene="hpnC"
                     /locus_tag="WIC03_000534"
     CDS             9815..10627
                     /gene="hpnC"
                     /locus_tag="WIC03_000534"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HEZ3883082.1"
                     /translation="MSVGHYENFPVGSLILPRRLRKPVHAVYAFARTADDMADEGSMP
                     SEARLAGLEGLRRELDVLASGGRSAHPLIARLDAEAVVPFGLDLQPFYDLLSAFSQDV
                     VKTRYGNFGELADYCRRSANPVGRIMLALYGKTDAVCVAQSDGICTALQLVNFWQDVA
                     VDWQKGRVYIPQDDLLKFGVSEGQIAEGRADAAFQRLMAYECRRAFRMLKAGSPLARE
                     LKGRIGLELRMIVLGAQLILQKLDACRYDVFAQRPVLDKKDWLIMLKRALWK"
     gene            complement(10698..11168)
                     /locus_tag="WIC03_000535"
     CDS             complement(10698..11168)
                     /locus_tag="WIC03_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HEZ3883083.1"
                     /translation="MYDVNTHDVRRFFARVWQQRLNPLQLSALEQKALRIVEAHPEYH
                     RYLERIEDHLDTDWLPENGESNPFLHMSLHLSVQEQAGIDQPHGIRAIHDTLCAKRGW
                     LEAEHEMMEALAETLWTAQRYGTGLDVNFYMTRLRKLIGLGAEDQARLNPHEIA"
     gene            11259..13100
                     /locus_tag="WIC03_000536"
     CDS             11259..13100
                     /locus_tag="WIC03_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HEZ3883084.1"
                     /translation="MQTNYLSETDSIMKKLICLFAVFLMLCGRAFALDANDLLPPEKA
                     FVPELAVADDGVNVRFKIADGYYMYQAKIVGKTDPADLLGQPSFSKGEEKEDEFFGRQ
                     TVYHHEAQVAFPYAKAVGEPYKLVLTYQGCAEAGVCYPPVDTEFDISGNGTYHPQTDE
                     PASAKDRFLQPSSQNGSGALPPPKGDEGGDSRFKLSWDTLNANLLAFFLAGLGLSFTA
                     CMYPLLPIVSSIVVGDKKAGKARAFVLSVVYVQGLALTYTLVGIVAGLTGALLTVWLQ
                     QAWVVLAASALMVVLALSMFGLFNIQLPNAVQSYFQNQSSRLSGGKIVSVFIMGILSA
                     LIVGPCVAPPLAFALGYIGQTGDAVLGGLALYTLALGTGVPLIAIGTFGGHILPKAGD
                     WMNAVKYAFGFILLAVAVYLATPHLPYYLVVALYTLLMLVPACMLLVNGRRQKRRPKA
                     VAFALGSILLIGGAWFGWQGANGKTTALHHFLTLNPPAEAGKSSEHGKMFADTAALKA
                     AMDTALKEHPDKPVVLDFYADWCISCKEMAAYTLNQPEVHQAVDMERFFQIDVTANTP
                     EHQALLKEYGLFGPPGVFVVRSDGSRSEPLLGFVKADKFIEWYEQNR"
BASE COUNT         3279 a         2957 c         3755 g         3466 t
ORIGIN      
        1 acgccgacgg aatttcgctt cgcattaact gccttatttt gaacgcacgt caaacttaaa
       61 tttgcaatgc cgtctgaaaa gaagatgggc tgtcggatac ggatgtccga ccattttatt
      121 agacctaacg tgggtttgag ataacacatt gattttttat gtagagcgat ttatttcaac
      181 ggcaatccat tccctctctc gtgggagagg gttaggggag ggcatcgggg cggtaacgga
      241 aatattgtct gtatctgccc cgttttaccc tctccccaac cctcccccac aggggaggga
      301 gtcaagttgc aggcacgccg acggaatatg gttgaaaccc ggcccttctg ggcgatggga
      361 ttttggttta ttttttggga agtaacccct tccaaatcag ggtaaacagg gctgtgcctt
      421 atgtgcggcc ctgtgtgttg aaacattgag gaatataacc gtgccgtcaa attactttat
      481 atacgcgcgg acgattgggt gctttgggcg gcatcgtgca acatcatacc gccttgtacc
      541 gcagcgtggc ggtatatgaa ccggctgtgc ttatcgtgcg gcgcggaagt aagaagctgc
      601 ggcgggtgag gcggtcgcgt tggcaggcgg acagacgttt gacgttatta atattcccga
      661 tctagacggc ctctatcagg cgcaatggat tgctttcgaa caggaaaccg tagaaaggtt
      721 cgccgcacaa tacgggacgg cgcaggcagt gtgcgatgcg gtgaagctgc cgcaccccgg
      781 gcggatgggc gcggcgtttg attatgcggc ggcggtgctg acggatgaag aagtgccgca
      841 caatgcggcg gaggtcgcgc tttgcggcgt gttggcgtgg ttgcagcatg acggcatcgg
      901 ttttgccgtg tacgaaggcg tcaacctgat gcggcaaatc cgcaaactga ttaccgccga
      961 tatggctgcg gactggtcgt cggcgatgtt ggcgcagcgg cttaattgca gcgaggccgc
     1021 gttgcggcgg cggacatcgg cgggaatgtc ggcgaccacc cagtgcatcc agcccagtcc
     1081 ggtcggcgat gttggcgcag cggcttaatt gcagcgaggt cgcgttgcgg cggtggacat
     1141 cggcgggaat gtcggcgacc acccggtgca tccagcccag tccggtcggc gcgtctttat
     1201 cgtaaacggt caggacgaaa ctttttgtcc cggcgggcgg atttttccac gacagcgcgg
     1261 gcgaagcatt gccgcccgaa cagccaaatc cgtaaggcgc gctcaaaagc tggttttgcg
     1321 tgaagccgcc gtcttcggac gggttgtcga attgcagcgt gaatgcgccc ccggcaaagg
     1381 cagcggttga aaaagccagc agtacggcgg agagaatgcg ggtttgcata gacggttcct
     1441 ttgcttgatg aataagtgga ttcattatag gctgtcaacg gcggcaggaa tgtgccgaaa
     1501 cggttggatg atgtgccgga atgcgcaaat gcaaaaatcg tggtttcaga cgagcctttg
     1561 caggtcgtct gaagtcttta attcctttcc ctgaggcttt agaaatatcg aatatttagg
     1621 tattgccgca acctaaccct caagatttac gtcatagccc gttcctgctc cggcgtgcgg
     1681 gaatggcaaa aattggggat tttgccgcca aatccatgat agagatacgg agcaaacgaa
     1741 tgattaccct gccttcctta aacaatctgc cgtcaaaatg ggatgaaatc cgctattggc
     1801 tcgaaacccg ggtttgtgag tgcggcgtta tgccgcatcc caatttgacg gaatgcgaag
     1861 ccgggcagtt cgatcgggat ttttgggaca atatcggctg cgcgcctgaa gaatatgtcc
     1921 gtatccgccg agccatccgt ctgttggaag cgcgttatcc cgacagtttg aatgagctgg
     1981 tgtgtgccgc cattgccacg cctttgggcg agatgctggc ggtgttcgga aataagggat
     2041 tgtgtctgct ggagtttgtc ggacagaagc atttggaaca ggaaattgcc gccgtccaaa
     2101 aagccttgcg cggacggttt gtgtttcggg aggatgagcg gacgcaactt ttgcggcagg
     2161 aattggactt atacttcaag ggtcgtctga aaacctttgc cacgcctttg gagcagattg
     2221 gcaccgagtt tcaaaagcag gcatgggacg cgctcttggc gattccttac ggcgaaacgc
     2281 gcagctacaa agagcaggcg cagcgtttgg gcaaccccaa cgccgtccgc gccgttgccg
     2341 ccgccaacgg gcagaacaag gtgtctatct tgattccctg ccaccgcgtc atcggcagcg
     2401 acggcaaact gaccggctac gcgggcgggt tgaaccgcaa acagttttta ctggctttgg
     2461 aacgcggcga agttcagacg gcattgtttt aagcggggga gggcagaacg gctcatcggg
     2521 cttaaatatg gtgtgatgac ggctgtggaa attcatacaa cggcaatcct tttaaggcat
     2581 cggtcttaat gtctttctga ttcatatgct tgtcgttttg tcgcccattt ttccaaaatt
     2641 aaggattgac acattttttc tggacgctat aatacgcccc attccccgat agctcagtcg
     2701 gtagagcgac ggactgttaa tccgcaggtc cctggttcga gcccaggtcg gggagccaga
     2761 attttgaagc ccgaacttga atgttcgggc tttttcatat ttgcacgtcc ggcgttcgga
     2821 atgcggtaag ataggttttc caacggtttc gcggtaaaat tctgcctgtc tgatttcggt
     2881 ttcttgttat gtccatcaaa atcctgatta tttctcccag ttggataggc gactgcgtga
     2941 tgacccagcc cttgttccgc cgtttgaagg aacttcaccc cggttgcacg attgatgtgt
     3001 tcgcaccgaa gtggtcgatg gcggtgtttg agcgtatgcc ggaagtgaat gagattcttg
     3061 aaaatccgtt cggacacggt gcgttggagt tgaaacgccg ttggcgggtc ggtagggatt
     3121 tggggcggcg cggatacgat caggttatcg tgttgcccgg ttctttgaaa tcggcaatca
     3181 tcgcgctggc gacaggtatc ggtaaaagga cgggttatgt cggtgaaagc cgttattttc
     3241 tgttgaacga tatacgcagg ctggataagg aacgtctgcc tttgatggtg gaccggtata
     3301 cggctctcgc gcatcagagt caggaagatt ttgacgggtg ttcaggattt cccgagtttt
     3361 ccattgatga acggcggcgg gaaatttctg tcgaaacttt cggtttgaat cttggaaagc
     3421 ctgttctggc tttttgtccg ggtgcggaat tcgggccggc aaagcgttgg ccgacaaggc
     3481 attttgctga gttgggcaaa cattattcgg cggcgggttg gcaggtttgg ctgttcggtt
     3541 cgcaaaaaga tgatgaaatc gccgaggaaa tcaaccgcct ttcagacggt atgtgtgtca
     3601 atttgtgcgg caaaaccgat ttgtcgcagg caatggattt gctgtcgttg gcagatacgg
     3661 ttgtgtgtaa cgacagcggg ctgatgcact tggcggcggc attgggcaga aaggtggtgg
     3721 cggtttacgg ttcttccagc ccgacgcata cgccaccttt gagcgacagg gcgaaaatcg
     3781 tcagcctgca cttggaatgt tcgccgtgtt ttaagcggga atgtccacta gggcatacag
     3841 actgcctcaa caggctgtat cccgagaaga ttgtgcaggc ggttgaagag gcggtatgat
     3901 gggttctgtt ttcgggttta tgccgtctga cggcggatat tgataggatg tttatggcta
     3961 ttgcgaacaa taaaaaagca tttcacgatt ttttcattga agaccggatt gaagccggtt
     4021 tggtcttgga aggctgggaa gtcaaagcaa tccgcgccgc gcgcgtacag cttaaagaga
     4081 gttatatcta ttggaaaaaa gacgcgtttt atctggtcgg ctgccatatt acggctttgc
     4141 ccacagcctc gacacacatt aaaccagatg ccgtacgtcc gcgcaagctc ttgttgaacc
     4201 agtcggaaat caacaaatta atcggcaaaa ccgaacgtgc cggctacacc atcgtgccgc
     4261 tggatttgca tttttcgcgc ggaaaaatca agatggaaat cggtttggca aaaggtaaga
     4321 aacagcacga caaacgtcag agtatgaaag aggcggattg gaaacgcgag aaacagcggt
     4381 tgattaagca tacgcgctga ttgtggaaac agtgcttcag acggtatggg caaggctgcc
     4441 gcctgatgtg taaacgttct acacctaaac cagcatccgc tttaatcagt acggcatgat
     4501 gccccaaacc ttcgtcaaag gtttgagcaa tttgatgata aattggcatt aaccaagggt
     4561 aaagtgcggt catttttcct gaaatttttg tgaataacga gggcaattat acacattttc
     4621 aaactggctt gacaaaatag caaaaagcaa acgtttacct ttaataaata agaaataaat
     4681 agagaaaaat gtgagctaaa tgacattttt tgttcagttt tatgctaaaa tccacgcccc
     4741 atttattttt acttgacaaa ggaaaattta ttatgaaact cgaagcaagc aagcaagcaa
     4801 gcagaagttt aaaaaatcat ttattataag tctatttttt tctattcttt atacctctcc
     4861 gcttttggct gttgattacg tttacgacaa aaccaagctc actgatgatg aaattacccg
     4921 cttaaaaaaa ctccgcgata gaaatagtga atattggaaa gaagaaactt atcttataac
     4981 cggggataat ccaaaagttc catccgtccc cgcactatac cctagaacat accaattcga
     5041 aaacatcaat aactcaaaaa agatttcttt ttatgatcaa aaatataccg aagattacct
     5101 agttggcttc gcccgaggtt taggggttgc aaaaagaaat ggggacacag aagaacaaat
     5161 acggaaatat tttaaggaat gttttaattc taacactaag atacgtgatt atagtacttg
     5221 ccaagctgaa aaatttggat tccatcctct tatagtaaaa agtcatatat tctccttagg
     5281 tccaaaaata aaaaatagtc atatcaacag cgaaatttta tctgtcggga attatacaga
     5341 atgggctaat caagtaattc atcatattga aaattatgtt tcttttgcag cacatctata
     5401 ttctggttta gatccattcc actatattga ggttacagac aactcgcacg ttatcgggca
     5461 aacgattagt ttggatgaat tccgcttaga aaattctctc tgggagcccc gttgggattc
     5521 taatgttggg aaattaaaaa caacgaatgc agacatccgt tttaatacta aaagtgaaag
     5581 tttactcgta aaagaagatt atgctggcgg agctcgtttt cgttttgctt acgatcctaa
     5641 agaagcgaaa aatactgcat taatttttga gaaaaatgtt actggcactt ccgacatcat
     5701 cttcgaaaat ccaatagacg atttaaaaag cctcgatggg catcaaatta tcaaagtaaa
     5761 tggcacagca gataaacacg cattccgtct ttctggcaaa caccaaaagg gaatttatac
     5821 gctttcttta caacaacgcc cagagggctt ttttaccaaa gtgcaagaac gcgatgatat
     5881 tgcgatttat gcacaacagg ctcaagccgc caatacttta ttcaacctac gtttgaataa
     5941 caaaaacaac gatattttcg accgcacttt accacgcaaa ggcttgtggt tgcgtgtgat
     6001 tgacggacat tccaaccaat gggtgcaagg caaaacagca cctgtagaaa gctatcgaaa
     6061 aggtgtgcaa ctcggcggcg aagtattcac atggcaaaac gaaagtaatc aactttctat
     6121 tggcttaatg ggcggacaag cagaacaacg cagtactttc cgcaacccag atacagacaa
     6181 tcttacaacg ggaaatgtga aaggctttgg tgcaggcgtt tacgccactt ggcatcaact
     6241 tcaggacaaa cagacaggtg cgtatgcgga tagctgggta caatatcaac gtttccgcca
     6301 tagcattaac actgaagatg ctacagaacg ctttacttca aaaggtatca ctgcctcaat
     6361 tgaagcgggt tacaatgctt tattggcaga acacttcact gaaaaaggca accgtgttcg
     6421 tttttacctg caaccacagg cacaattgac ttatttgggg gtaaacggaa aattcagcga
     6481 tagcgaaaat gcccaagtga atttgcttgg cttccgccaa ttacaaagcc gagtgggcgt
     6541 tcaagctaaa gctcaatttg ctttcactaa tggcgtcact ttccaaccat ttgttgctgt
     6601 caattcaatc taccaacaaa aacctttcgg tctggaaatg gacggagagc gtcgagtgat
     6661 aaacaataag acggcaattg agagccaatt aggtgtagca gtgaaaatca aatctcactt
     6721 aactttacaa gcaacattca accgccaaac aggcaaacat catcaggcta aacaaggtgc
     6781 gttgaattta cagtggacgt tttaagttaa gttgtaacaa aataaaatcg gcagagaaat
     6841 ttactgccga ttttttattt taaaaatgcg gtcatttttc gtgtcatttc caccaatttt
     6901 ttaccgcact cacgagctcg cgctaaaccg acaatcggat ctattgtccc tttctttgtt
     6961 gtgtatgcaa atcagaaatg ccggttacgg aagcagtttg cccggattca taatgttata
     7021 tggatcaagg tgttgtttga tgcttttcat cagggcgatt tcggcaggcg tgcgtacttt
     7081 gtccagccac tgttttttga tgatacctat gccgtgttcg gcggcaatcg tgccgttgca
     7141 ggcaagaacg ttgcgataaa ctgcgtcgtt gatgtcgttt tcgtaacgat agacttcatt
     7201 gctgaggatt tcgggcagga aagtattgta gtgcaggctg ccgtcgccaa gatgtccgaa
     7261 gcagacgatt tgtatgcctt taaaattctg ttccaaatct ttggcgcacc ggcggacaaa
     7321 gtcggcaacg cgtccgatag gaatggcaat atcgtgtttg atgctggtgc ccagtttgcg
     7381 ttgcgatgcg gagatgtttt cgcgcaatgc ccacatatgg atacgttctt gttcgctttg
     7441 tgccaatacg ctgtcggtaa atcctttttt ataaagaaat tcgacaagcc gatcatcaag
     7501 attgctgtcg ggtaatgagt cggtcaactc aagtaaaata tgccattctg aatgtgtcgg
     7561 cagggggaat ttgctgaatt cggaagacaa ctcggcggca aaacggccga tcagctcaaa
     7621 actgcatagg cgttcggcaa agtgagcttg ggtttcggtc agcaggcgga cggcggattc
     7681 gatgtcgggt atgccgaccc atgccgttgc cttgtccgag ggacgggcaa aaagcttgag
     7741 cgtggcggca gtgatgatgc ccagtgtgcc ttcgctgccg ataaacagat ggcgcaggtc
     7801 gtagccggtg gtgtttttat gcaggggatg gagatgggaa accagctcgc cgttggggag
     7861 gacgacttcc aaaccgataa ccaggtcgcg catcgtgccg taacgcaata cgttcaaacc
     7921 tccggcattg caggcgatgt tgccgccgat ttgacaggag ccttcgctgg cgagactgag
     7981 tgggaacagc ctgtttgagg cttcggctgc ctgttggacg gtttggagta cggaacctgc
     8041 ttcgacggtt atgcagttgt ctgacaaatt gatgctgcgg atgcggttga gtttggaaag
     8101 gttcagcaat acgccgtttt ccgatactgc cgcgccgcac aaaccggtat tgccgccttg
     8161 cggcgtaacc ggaatgcggt gttcgtaaca aaaacgcata atggtttgca cgctttcaac
     8221 gctgcgcggc tgcaaaatga tgtctggtgc agacgtaaag cggttgcgct ggtcttttaa
     8281 aagcgtcgga gaaagttcgg caatttcatc tgccggcagg agtcgggaaa attcggtatg
     8341 aaggtcaggc atgtttgtcg gaagggtagg gagaagattg aggaagggaa tctgatgccg
     8401 tctgaaaaat caaaaaagca ggatactgaa cgtatcctgc ttttttgcaa gcggaaaatg
     8461 cttattttgc agcttcggca gcaggagctt cggcagcagg agcttcagcg gcaggtgctt
     8521 cggtagcggc agcttcagcg gcaggtgctt cggtagcggc agcttcagca gcgggagctt
     8581 cggcggcagg tgcttcggta gcgggagctt cagcggcagg tgcttcggta gcgggagctt
     8641 cagcggcagg tgcttcagta gcgggagctt cagcggcagg tgcttcggta gcgggagctt
     8701 cagcggcttt ttcaccgccg caggctgcca ggaccaaaga caacaaagca gcggcaaaca
     8761 gggatttttt cattttagta acctttagaa ccaatttaaa ttattgaaac aaataggtct
     8821 ttatcgacca tactacaaac cgggttggaa attcatcgct gaatttgcgt aattatgcac
     8881 tatggaataa gggattgcaa tgtcgtacag atgggaaaaa ggtttgaatg aaaagttttc
     8941 tggcgggcgg attgataaat cctgtaaaac ggttttgctg ttaaaaattc gatagggata
     9001 aatattatag agattaatat aatttcagta tcttgtatga tttattaagg cggattggga
     9061 aagtcggcag acaggtcgga atggttttaa taatgttgta aaaaagttac atcgcaataa
     9121 aataacaaaa atttacattg aaatatgtgc tgtacagttt atgaacaggt taacggtaat
     9181 cggccggcgc ggcggttgtg ttagaatgcg cctgtaatca atctgctgtt cctgaaagga
     9241 caaatatggc tatcgtgaag aattccgtgg tttcgctgca ttatgagatg tatgatgcca
     9301 acaatcagct tttggacaaa accgaagaac cgattgcgta tctgcacggc ggttacgacg
     9361 gcattttccc tttggtggaa gaggcgttgc acggtaagga tgccggcgat acggtcgatg
     9421 tggcgctttc gcccgacgat gcgttcggcg agcaggatcc ggagttggtc cgtattgaag
     9481 atgcgggcgt gttccctgtt gaagtcgaag tcggcatgat gtttgaagcc gacgatcctg
     9541 aaaccggcga tgttgtcgtc tatcgtgtaa ccgatgttgc cgacggtaag gcggtggtgg
     9601 acggcaacca tcctttggca ggcatgaaaa tccgctttaa ggctacggtt gaaagtgtgc
     9661 gcgatgcatc cgatgaggaa atcgcacacg gtcatgtcca cggcccgcac ggtcatcacc
     9721 accactgatt gccggcagct tggaggtatc tgatgccgtc tgaaaagggt ttgttccatt
     9781 tcagacggca ttttgtttga tggatggaga aggtatgtcg gttgggcatt atgaaaattt
     9841 tcccgtcggt tcgctgattt tgccgcgcag gttgaggaag ccggttcacg cggtgtacgc
     9901 gtttgcacgg acggcggacg atatggcgga cgagggcagt atgccgtctg aagccaggtt
     9961 ggcggggttg gagggtttgc ggcgcgagtt ggacgtgttg gcatcgggcg gccggtcggc
    10021 gcatcctttg attgcacgat tggatgccga ggcggttgtg ccgttcggtt tggatttgca
    10081 gccgttttat gatttgcttt ctgcattttc tcaggatgtg gttaaaacaa ggtatggaaa
    10141 tttcggcgag ctggccgatt attgccgacg ttctgccaat cctgtcggac gcattatgct
    10201 ggctttgtac gggaaaacgg atgcggtgtg cgtggcgcaa agcgacggca tctgtacggc
    10261 tttgcaactg gtgaattttt ggcaggatgt ggctgtggat tggcaaaagg gcagggttta
    10321 tattccgcag gacgatttgt tgaaattcgg tgtttccgaa ggacagattg cggagggtag
    10381 ggcggatgcg gcgtttcagc ggctgatggc gtatgagtgc cggcgcgcat ttcgtatgct
    10441 gaaggcgggt tcgcctttag cgcgcgaatt gaaagggcgt atcggtttgg aactccgtat
    10501 gattgtgtta ggggcgcagt tgattttgca gaaactggac gcgtgccgat acgatgtgtt
    10561 tgcacaacgc cccgttttgg ataagaagga ttggttgatt atgctgaaac gcgcgttgtg
    10621 gaaatgatgg gggaaatgca tgccgtctga aaggggttgt tccgcttcag acggcatttt
    10681 gcaggcggtt ggtatggtca ggcgatttca tgtgggttca acctggcttg atcttctgca
    10741 cccaagccga tgagtttgcg cagtcgggtc atgtagaaat tgacatccaa accggtgccg
    10801 tagcgttgcg ccgtccacag tgtttccgcc agtgcctcca tcatttcgtg ttcggcttcc
    10861 agccagccgc gtttggcgca cagggtgtcg tggattgcgc gtatgccgtg cggctggtct
    10921 atgcccgcct gttcttggac ggacagatgc agcgacatat gcaggaaggg gttgctttcg
    10981 ccgttttcgg gcagccagtc ggtgtccaga tggtcttcga tgcgttcgag atagcggtgg
    11041 tattcgggat gggcttcgac aatgcggagg gctttctgtt ccagtgcgct cagttgcagc
    11101 ggattgagcc gctgctgcca cacgcgggcg aaaaagcggc ggacatcgtg ggtattgaca
    11161 tcgtacatgg cgggttcgat aagatggtta tggaaatagg ttcggataat ggcgggtttt
    11221 acataaatta aatcccaccg tcaaccttac catttataat gcagacaaat tatttgtctg
    11281 aaacggattc gattatgaaa aaactgattt gcctgttcgc cgtatttttg atgttgtgcg
    11341 gacgagcttt cgcgctggat gcgaacgatc tgctgccgcc ggaaaaggca ttcgtgccgg
    11401 agcttgccgt tgccgacgac ggtgtgaacg tccgtttcaa gattgccgac ggatactata
    11461 tgtatcaggc gaaaatcgtc ggcaagaccg atccggcgga tttgttggga cagccttctt
    11521 tcagcaaggg cgaagagaag gaagacgagt ttttcggcag gcagacggtt taccatcacg
    11581 aagcgcaggt tgcctttcct tatgcaaagg ctgtcggcga accgtataaa ttggttttga
    11641 cctatcaggg ctgtgccgaa gccggcgtgt gctatccgcc cgtggatacc gagtttgata
    11701 tttccggcaa cggcacttac catccgcaaa ccgacgaacc ggcatccgcc aaagaccgct
    11761 ttttgcagcc ttcctctcaa aacggcagcg gggcgctgcc gcccccgaag ggggatgagg
    11821 gcggcgacag ccgtttcaag ctgtcttggg atacgctcaa cgccaatctt ttggcgtttt
    11881 ttctcgccgg tttgggtctg agttttaccg cctgtatgta tcccctgttg ccgattgttt
    11941 ccagtattgt ggtcggcgac aaaaaggcgg gcaaggcgcg ggcgtttgtg ctgtccgtcg
    12001 tttatgttca gggtttggct ctgacttata cgctggtcgg cattgttgcc ggactgacgg
    12061 gcgcactgct gaccgtatgg ttgcagcagg cttgggtcgt gttggcggca tcggctttga
    12121 tggtcgtctt ggcactgtct atgttcgggc tgttcaacat ccagcttccc aacgccgtgc
    12181 agtcgtattt tcagaatcaa agcagcaggc tttcaggcgg taaaatcgtt tccgtcttta
    12241 ttatgggcat attgtccgcg ctgattgtcg ggccgtgcgt cgccccgccg ctggcatttg
    12301 ctttgggcta catcggtcag acgggcgatg cggttttagg cggtttggca ctttacactt
    12361 tggcgttggg caccggcgtt ccgctgattg ccatcggcac gttcggcggg catatcctgc
    12421 ctaaggcagg cgattggatg aatgccgtca aatacgcttt cggctttatc ctgcttgccg
    12481 tcgccgttta cctcgccacg ccgcacttgc cctattatct cgtcgtcgcg ctgtacacgc
    12541 tgctgatgct ggttcctgcc tgtatgctgc tggtcaacgg acgcaggcag aaacgccgtc
    12601 cgaaagctgt ggcattcgca ttgggcagca tattgctgat aggcggcgcg tggttcggct
    12661 ggcagggcgc aaacggcaaa acgaccgcgc tgcaccattt cctgaccctc aatccgccgg
    12721 ccgaagcagg caaatcttcg gaacacggca aaatgtttgc cgatactgcc gcgctgaagg
    12781 cagcgatgga tacggcgttg aaagaacatc ccgacaaacc cgtcgttttg gatttttatg
    12841 ccgactggtg catttcctgc aaagaaatgg cggcttacac gctcaatcag ccggaagtgc
    12901 atcaggcagt cgatatggaa cgctttttcc aaatcgacgt aaccgccaac acgcccgaac
    12961 atcaggcgtt gttgaaagaa tacggtctgt tcgggccgcc gggcgtgttt gtcgtccgct
    13021 ccgacggcag ccgcagcgag ccgctgctgg gttttgtcaa agcagacaag tttatcgagt
    13081 ggtatgaaca aaaccgctga ttccgatgtt tcaaatgccg tctgaagttc tcagacggca
    13141 tttttgtcag aaaggaaaac ggcaatatcc gctatacaca cggaagcagt ccaaagatgt
    13201 gaaaaccgtg tgtataagtt agtttgcgta ttgaaattaa aagataaaaa tacgatgatt
    13261 aaaaaatagg cataagaaaa gataaacaca gccggtgttt gccggtgtgt gtatcttttg
    13321 tggaaaaaca tcttaatgtc atgtatttta tggaaaataa taacatgatt aaaaaatagt
    13381 caatcattaa ggttcaggtg ggcggctgca accgtcaatg tctgaaaaga ggcagtgctg
    13441 tttaagtatg gaaagtc
//
LOCUS       DAWHSK010000026        13426 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.026, whole genome shotgun sequence.
ACCESSION   DAWHSK010000026 DAWHSK010000000
VERSION     DAWHSK010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13426
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.026"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..1186)
                     /locus_tag="WIC03_000537"
     CDS             complement(<1..1186)
                     /locus_tag="WIC03_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase-like protein"
                     /protein_id="HEZ3883085.1"
                     /translation="MSTFPNHTMHTISFPNRTRLTALPPLSLYIHIPWCIKKCPYCDF
                     NSHSLKNGLPEAAYIDALLTDLQLELPNIWGRPVETIFFGGGTPSLFQAESIDRLLSG
                     VRSLLRLQPEAEITLEANPGTFEIEKFQGFKDAGITRLSIGVQSFNNDMLSRLGRVHN
                     GKEALTAIDTALKLFDKVNIDLMYALPNQTVQTALDDVQTAIATGATHISAYHLTMEP
                     NTPFGHTPPKGLPQDEAALDIEDAVHGALEGAGFVHYETSAFAKPAMQCRHNLNYWQF
                     GDYLGIGAGAHGKISYPDRIERTVRRRHPNDYLALMQSQPSEAVERKTVAAEDLPFEF
                     MMNALRLTDGVPAAMLQERTGVPSAKIMAQIETARQKGLLETDPAVFRPTEKGRLFLN
                     DLL"
     gene            complement(1219..3744)
                     /gene="ligA"
                     /locus_tag="WIC03_000538"
     CDS             complement(1219..3744)
                     /gene="ligA"
                     /locus_tag="WIC03_000538"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEZ3883086.1"
                     /translation="MNPNSKHNTNLTNLLKPSDSDIKQFAAQHIRRLTDLLNRYAYEY
                     YTLDAPSVPDAEYDKLFRELEALELNHPELKLPDSPTQRVGGEPLAGFAEVRHEVPML
                     SLTNAFSPQDENGVFDHAEMYAFDQRVRDGLDGGNPEYVIEPKFDGLAISLLYRDGIL
                     VQAATRGDGTTGEDVTQNVKTVANIPLRLHGENPPELIEVRGEVLMLKADFAALNKRQ
                     AENGQKPFANPRNAAAGSLRQLDSRITAQRKLHFFPYSVARQQGGFVAEEHIQELAYF
                     QALGFSLPNGNFGCFKNIDEVLAFYEQMQQKRPELPYEIDGMVVKVNSLAQQRELGFI
                     SRAPRWAIAHKFPAEEALTIVEAIDVQIGRTGAVTPVARLQPVFVGGVTVTNATLHNQ
                     DEVSRKDVRVGDTVVVRRAGDVIPEVVRVIFERRPMQETAVAVSDGIGHRQDDLFAEA
                     PAANQTQSVPLYKPYRLPTHCPICRSEIEREEGEAVARCSGGMLCQAQRAQGLIHFAS
                     RKAMDIDGLGEKQIEQLVAQDLVRHFADLYRLDIPTLQKMKETADKTVAESDQMPSEG
                     SSVGASGKHKKQPTKWAENILAGIESGKTPELARFLFALGIRHVGERTAKTLAQAFGT
                     LERVRRAPEPVLACLPDIGTVVAHSIAHFFAQAEQQAMIDELLAAGVAPQTQAVTIPP
                     ARHAEPQRWIARLPDFKISENKAQALWELAGKNIEGLQNDKALPADWQAWRSEPQNAA
                     LLENLKTFFAQMPSEDEAAQGSDGINKAVAGKTFVLTGTLPTLKRDQAQALIEAAGGK
                     VSGSVSKKTDYVVAGEAAGSKLEKANALGVSVLSEAELLTLLG"
     gene            complement(3892..5178)
                     /locus_tag="WIC03_000539"
     CDS             complement(3892..5178)
                     /locus_tag="WIC03_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HEZ3883087.1"
                     /translation="MIYIVLFLAAVLAVVAYNMYQENQYRKKVRDQFGHSDKDALLNS
                     KTSHVRDGKPSGGSVMMPKPQPAVKKTAKLQDPAMRNLQEQDAVYIAKQKQAKASPFK
                     TEIETALEESGIIGNSAHTVSEPQTGHSAPKPADAPAKPVPVPQTPAKPLITLKELSK
                     VELPWFDVRFDFISYIALTEAKELHALPRLSNRCRYQIVGCTMDDHFQIAEPIPGIRY
                     QAFIVGIQAVSRNGLASQEELSAFNRQVDAFAQSMGGQTLHTDLAAFIEVASALDAFC
                     ARVDQTIAIHLVSPTSISGVELRSAVTGVGFVLEDDGAFHYTDTSGSTMFSICSLNNE
                     PFTNALLDNQSYKGFSMLLDIPHSPAGEKTFDDLFMDLAVRLSGQLNLNLVNDKMEEV
                     STQWLKDVRTYVLARQSEMLKVGIEPGGKTALRLFS"
     gene            complement(5372..5944)
                     /gene="ampD"
                     /locus_tag="WIC03_000540"
     CDS             complement(5372..5944)
                     /gene="ampD"
                     /locus_tag="WIC03_000540"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HEZ3883088.1"
                     /translation="MDNPATAPWQDGWLQSVRHIPSPNFGPRESKEEVSLVVLHNISL
                     PPFEYGTDAVEKLFANRLDPDGHPFFSLIHTLRVSSHFLIKRDGETVQFVSCDNMAYH
                     AGVSSFGGREKCNAFSIGIELEGCDFEPFTEAQYRSLETLLDAICRRYPITAVTGHQD
                     IAPGRKTDPGHFFDWRRIRGKGFPVNRNAV"
     gene            6029..7024
                     /gene="mltG"
                     /locus_tag="WIC03_000541"
     CDS             6029..7024
                     /gene="mltG"
                     /locus_tag="WIC03_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEZ3883089.1"
                     /translation="MLRKLLKWSAVFLTVSAAVFAALLFVPKDNGRAYRIKIAKNQGI
                     SSVGRKLAEDRIVFSRHVLTAAAYVLGVHNRLHTGTYRLPSEVSAWDILQKMRGGRPD
                     SVTVQIIEGSRFSHMRKVIDATPDIEHDTKGWSNEKLMAEVAPDAFSGNPEGQFFPDS
                     YEIDAGGSDLRIYQIAYKAMQRRLNEAWESRQDGLPYKNPYEMLIMASLIEKETGHEA
                     DRDHVASVFVNRLKIGMRLQTDPSVIYGMGAAYKGKIRKADLRRDTPYNTYTRGGLPP
                     TPIALPGKAALDAAAHPSGEKYLYFVSKMDGTGLSQFSHDLTEHNAAVRKYILKK"
     gene            7084..7704
                     /locus_tag="WIC03_000542"
     CDS             7084..7704
                     /locus_tag="WIC03_000542"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEZ3883090.1"
                     /translation="MKPQFITLDGIDGAGKSTNLAVIKAWFERRGLPVLFTREPGGTP
                     VGEALREILLNPETKAGLRAETLMMFAARMQHIEDVILPALSDGIHVVSDRFTDATFA
                     YQGGGRGMPSEDIEILEHWVQGGLRPDLTLLLDVPLEVSMARIGQSREKDRFEQEQAD
                     FFMRVRSVYLNRAAACPERYAIIDSNRSLDEVRNNIEKVLDGHFGC"
     gene            7926..9206
                     /locus_tag="WIC03_000543"
     CDS             7926..9206
                     /locus_tag="WIC03_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme"
                     /protein_id="HEZ3883091.1"
                     /translation="MENSLKEAALKFHELPVPGKISVTPTKSLATDKDLALAYSPGVA
                     APCMEIHADPQNAYKYTAKGNLVAVISNGTAVLGLGDIGALAGKPVMEGKGVLFKKFA
                     GVDVFDIEIDEKDPQKLVDIIAALEPTFGGINLEDIKAPECFYIERELRKRCKIPVFH
                     DDQHGTAIITAAAVLNALRFTGRKIEEATLVCSGAGAAAIACLNQLLDLGLKRENVTV
                     CDSKGVIYQTREDKDRMDESKKFYAVEDNGQRVLADAVKGKDIFLGLSGANLLTPEML
                     NTMNEKPIVFAMANPNPEILPPLAKETRPDVVIGTGRSDFPNQVNNVLCFPFIFRGAL
                     DVGATTINEEMKRACVYALADLAMEEVTEEVVAAYGKKFEFGAEYLIPTPFDSRLLPR
                     VATAAAKAAMESGVATRPIADFEAYAAKLGEWKL"
     gene            9289..9560
                     /locus_tag="WIC03_000544"
                     /pseudo
     CDS             9289..9560
                     /locus_tag="WIC03_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713445.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9600..10634
                     /locus_tag="WIC03_000545"
     CDS             9600..10634
                     /locus_tag="WIC03_000545"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025462052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEZ3883092.1"
                     /translation="MPNCFKFHTVIERHWQKPYPVLSFLLKPLSGLFAKIAAKRRTDF
                     LSGKRQSEKLPVPVVVVGNIHAGGTGKTPIVAALVSGLQEKGVKVGIISRGYGRKSKA
                     VHVLNAESRAEDAGDEPLLLFRKTGAPTAVGSSRAEAGRALLAAHPDIGLIVADDGLQ
                     HYALRRDVEIAVFPAADTGRTDLDLLPNGSLREPLLRLDSVDAVVVSGGKADALFRPS
                     ENMFHSRIEAGRIYRLNNPSEILDTGRLKNQTVVAVAGIAKPARFFDSLRNMGITVKR
                     TVALPDHADISAADLPDADAVIITEKDAVKFSDGICTDNVWVLPVCAIIEPDLAAFVL
                     ERLEDVPKAV"
     gene            10840..11421
                     /locus_tag="WIC03_000546"
     CDS             10840..11421
                     /locus_tag="WIC03_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HEZ3883093.1"
                     /translation="MKLKTLLLPFATLALCTNAFAAPPSDASLASWLDTQNFDRDIEK
                     NMIEGFNAGFKPYADKALAEMPEAKKDQAAKAFNRYRENVLKDLITPEVKQAVRNTLL
                     KNAREIYTQEEIDGMIAFYGSPVGQSVVAKNPRLIKKSMSEIAVSWTALSGKIARHHL
                     PAFTEELRRIICGGKNPDAGCKQAGQVGKRHQK"
     gene            11490..11672
                     /locus_tag="WIC03_000547"
     CDS             11490..11672
                     /locus_tag="WIC03_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEZ3883094.1"
                     /translation="MEKKFLDILVCPVTKGRLEYHQDKQELWSRQAKLAYPIKDGIPY
                     MLENEARTLSEEELKA"
     gene            11669..12430
                     /gene="kdsB"
                     /locus_tag="WIC03_000548"
     CDS             11669..12430
                     /gene="kdsB"
                     /locus_tag="WIC03_000548"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEZ3883095.1"
                     /translation="MTEFVVLIPARLDSSRLPRKALADIHGKPMVVRVAEQAAKSKAA
                     RVVVATDHPDIQTVCQAHGVEVVMTSNRHESGTTRLAEASAALKLPPHLVVVNVQGDE
                     PLIAPELIDRTAEVLVENNVQMATAAHELHDFDELMNPNAVKVVLDKNRNAIYFSRAP
                     IPYPRDAMRAGKREMPSETAVLRHIGIYAYRAGFLQRYAEMSVSPLETIESLEQLRVL
                     WHGYPIAVETAKEAPAAGVDTQEDLDRVRAVFQTV"
     gene            12453..12770
                     /locus_tag="WIC03_000549"
                     /pseudo
     CDS             12453..12770
                     /locus_tag="WIC03_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237659.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
     gene            12875..13072
                     /locus_tag="WIC03_000550"
     CDS             12875..13072
                     /locus_tag="WIC03_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883096.1"
                     /translation="MAHGHKARADGLAQQPDKAAEANEEEYLTKALSQNLLSTLDVAL
                     ARFPEDAWFQEIKQDAQKHFA"
     gene            13229..13321
                     /locus_tag="WIC03_000551"
     tRNA            13229..13321
                     /locus_tag="WIC03_000551"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13263..13265,aa:Ser,seq:gct)
BASE COUNT         2992 a         3346 c         3924 g         3164 t
ORIGIN      
        1 gcagcaaatc gtttaaaaac aagcgtcctt tttcggtcgg gcggaatacg gcggggtcgg
       61 tttccagcag gcctttttgc cttgccgttt cgatttgcgc catgattttg gcactcggta
      121 cgcccgtgcg ctcctgcaac atcgcggcgg gtacgccgtc ggtcaggcgc agggcgttca
      181 tcataaactc gaacggcaaa tcttcggcgg caacggtttt gcgttcgacg gcttcactcg
      241 gttggctttg cattaaggcg aggtagtcgt tggggtggcg gcggcggacg gtgcgctcga
      301 tgcggtcggg ataggaaatt ttgccgtgag cgcccgcgcc tatgcctaaa taatcgccga
      361 actgccagta gttcaaattg tggcggcact gcatggctgg tttcgcaaaa gccgatgttt
      421 cgtagtggac aaaacccgcg ccttccagcg caccgtgtac cgcgtcttcg atgtcgagcg
      481 ccgcttcgtc ttgcggcaaa cctttcggcg gcgtatgacc gaacggcgtg ttcggctcca
      541 tcgtcagatg atacgcgctg atgtgggtcg cacccgtagc gatggcggtt tgcacgtcgt
      601 ccaatgccgt ctgaacggtc tggttcggca gggcatacat caagtcgata ttgactttat
      661 caaataattt caaggcggtg tcgatggcgg ttaaggcttc tttgccgttg tggacgcgcc
      721 ccagccttga gagcatatcg ttgttgaaac tctgcacgcc aatagaaagc cgcgtaatac
      781 ctgcgtcttt aaatccttga aacttctcga tttcaaaggt gcctggattg gcttccaacg
      841 taatttccgc ctccggctgc aagcgcaaca gtgaacgcac gccgctcaac aaacggtcaa
      901 tcgattccgc ctgaaacagg ctgggcgtac cgcctccgaa aaagatcgtt tccaccggcc
      961 tgccccaaat attgggcaat tcaagctgca agtcggtcag cagcgcgtcg atataggcgg
     1021 cttcgggcaa tccgtttttc aggctgtggg aattaaagtc gcaatacggg cattttttga
     1081 tgcaccacgg gatgtggata tagagtgaca ggggcggcag ggcggtgagc cgggtgcggt
     1141 ttggaaagga aatggtgtgc atggtgtggt tcggaaaagt ggacaatgcc gtctgaaggc
     1201 ggttcagacg gcatgggttc agccgagcag ggtaagcagt tcggcttcgc tgaggacgga
     1261 aacgcccaag gcattggctt tttccagctt gctgcccgcg gcttctccag cgacgacgta
     1321 atcggttttt ttggaaacgc tgccggaaac tttgccgcct gcggcttcga ttagggcttg
     1381 ggcttggtcg cgtttgagcg tgggcagcgt gccggttaat acaaaggttt tgcctgctac
     1441 ggctttattg atgccgtctg aaccttgcgc cgcctcgtct tcagacggca tttgcgcgaa
     1501 gaaggttttt aggttttcga gcagggcggc gttttgtggt tcgctgcgcc atgcttgcca
     1561 gtcggcgggg agggctttgt cgttttgtag accttctatg tttttaccag cgagttccca
     1621 taaggcttgg gctttgtttt cgctaatttt gaagtcgggc aggcgggcga tccagcgttg
     1681 cggttcggcg tggcgggcgg gcgggatggt aacggcttgg gtttgcgggg caacgcctgc
     1741 ggcgagcagt tcgtctatca tcgcctgctg ttcggcttgg gcgaagaagt gggcaatgga
     1801 atgcgccact accgtaccga tgtcgggcag gcaggcgagg acgggttcgg gggcgcggcg
     1861 gacgcgttcc aatgtgccga atgcctgcgc cagcgttttg gcggtgcgtt cgccgacgtg
     1921 gcggatgccg agcgcgaaca ggaagcgcgc gagttcgggc gttttgccgg attctatgcc
     1981 tgcgaggatg ttttccgccc acttggtcgg ttgtttttta tgtttgcccg acgcgccgac
     2041 cgaactgcct tcagacggca tttgatccga ttcggcaacg gttttgtccg ccgtttcctt
     2101 cattttttgc agggtcggaa tatcgaggcg gtagaggtcg gcgaagtggc ggacgaggtc
     2161 ttgggcgaca agctgttcga tttgtttttc acccaagccg tcgatgtcca tcgctttgcg
     2221 cgaggcgaag tggattaagc cttgcgcgcg ttgtgcctga caaagcatgc cgccgctgca
     2281 tcgggcgacg gcttcacctt cttcgcgttc gatttcgctg cggcagatgg ggcaatgggt
     2341 cggcaggcgg tagggcttgt agagcggaac ggattgggtt tggtttgcag ccggtgcttc
     2401 ggcaaacaaa tcgtcctgcc gatgcccgat gccgtctgaa acagcaacgg cggtttcctg
     2461 catcgggcgg cgttcaaaaa tcacacgcac gacttcggga atcacgtctc cagcgcggcg
     2521 cacgacgacg gtatcgccga cgcgcacgtc tttacgcgat acttcgtcct gattgtgcag
     2581 ggtggcgttg gtaacggtta cgccgccgac gaatacgggt tgtaatcggg caaccggcgt
     2641 taccgcgccc gtcctgccga tttgcacgtc aatcgcttcg acaatggtca gggcttcttc
     2701 ggcagggaat ttgtgggcaa tcgcccagcg tggcgcgcgt gagatgaagc cgagttcgcg
     2761 ctgttgcgcc aagctgttga ctttgaccac cataccgtcg atttcgtagg gcagttcggg
     2821 gcgtttttgc tgcatttgtt cgtaaaacgc caatacttcg tcgatatttt tgaaacagcc
     2881 gaaattgcca tttggcagac tgaagccgag tgcttggaaa taggcgagtt cttggatgtg
     2941 ttcttccgcg acgaaaccgc cttgctggcg ggcgacggag taggggaaaa agtgcagttt
     3001 gcgttgcgca gtgatgcgcg aatcgagttg gcgcaggctg ccggcggcgg cgttgcgtgg
     3061 gttggcaaag ggtttttgcc cgttttcggc ttggcgttta ttgagcgcgg caaagtcggc
     3121 tttgagcatc agcacttcgc cgcgcacttc tatgagttcg ggtggatttt caccgtgcag
     3181 tcgaagagga atattggcga cggttttgac gttttgggta acgtcttcgc ccgtcgtgcc
     3241 gtcgccgcgt gtcgccgcct gcaccaatat gccgtcgcgg tagagcaggc tgatggcgag
     3301 gccgtcgaat ttgggttcga taacgtattc gggattgccg ccgtccaaac cgtcgcgcac
     3361 gcgttggtcg aaagcgtaca tttcggcatg gtcgaacacg ccgttttcat cttgcgggga
     3421 gaaggcgttg gtcagcgaca gcatcggcac ttcgtggcgc acttcggcaa atcccgccaa
     3481 aggctcgccg ccgacgcgct gagtcgggct gtcgggcagc ttgagctcgg gatggttcag
     3541 ctcgagggct tcgagttcgc ggaacaattt gtcgtattcg gcatcgggta cgctgggtgc
     3601 gtcgagggtg tagtattcgt aggcgtagcg gttgaggagg tcggtgagac ggcggatgtg
     3661 ttgtgcggca aattgtttta tatcactatc agacggttta agaagattgg taaggttagt
     3721 gttatgtttt gagtttggat tcatgagaga aggttttcag acgacctttg tctgatacgg
     3781 gatgaaacgg gcaaaggtcg tctgaaaaat gataggttga aaacagctga attttacccg
     3841 aaaaaaagcg gatatgccgt aacgacatat ccgctttgat tgcattcgat tttaggagaa
     3901 caggcgcaat gcggttttgc cgcccggttc gataccgact ttgagcatct cggactgacg
     3961 agccaataca taagtgcgca cgtctttgag ccattgggtc gaaacttctt ccattttgtc
     4021 gttgaccaga ttcaggttca actggccgga caggcgtacc gccaaatcca taaacaaatc
     4081 gtcgaaggtt ttttcgcctg ccggagagtg cgggatgtcg agcagcatac tgaagccttt
     4141 gtaggactgg ttgtccaaaa gggcattggt aaacggctcg ttgttgagcg agcagatgga
     4201 gaacatggtc gagcccgacg tgtcggtata gtggaacgcg ccgtcgtctt ccaggacgaa
     4261 acccacgccc gttacggcgg aacgcagttc tacgccgctg atgctggtcg gggaaaccaa
     4321 atggatggcg atggtctggt cgacgcgcgc gcagaatgcg tccagtgcgg aagccacttc
     4381 gataaaggcg gcaaggtcgg tgtgcagcgt ctgaccgccc atgctttgtg cgaatgcgtc
     4441 cacctggcgg ttgaatgcgg agagttcttc ctgcgaggca agtccgttgc ggctgactgc
     4501 ctgaataccc acgataaatg cctgatagcg gatgcccggg atgggttcgg caatctggaa
     4561 atggtcgtcc atggtgcagc cgacaatctg gtagcggcag cggttggaaa ggcgcggcag
     4621 tgcgtgcagt tctttggctt cggtcagcgc gatataggag atgaaatcga agcgcacgtc
     4681 aaaccacggc agttcgactt ttgacagctc tttgagcgta atcagcggtt ttgccggcgt
     4741 ttgcggaacg ggaacgggtt ttgccggcgc gtcggcaggt ttcggtgcgg aatgtccggt
     4801 ttggggttcg gaaacggtgt gggcggagtt gccgataatg ccgctttctt ccaaggcggt
     4861 ttcgatttcg gttttgaacg gggaggcttt tgcctgtttc tgcttggcga tgtagacggc
     4921 atcctgttct tgcaggttgc gcatagcagg gtcttggagt tttgccgttt ttttgaccgc
     4981 cggttggggt ttcggcatca tgactgaccc gccggacggt ttgccgtcgc ggacatggct
     5041 ggttttgctg ttgagcaggg catctttgtc ggagtgcccg aactggtcgc gcactttttt
     5101 gcggtattgg ttttcctgat acatattgta ggcgacaacg gcgaggacgg cggcgaggaa
     5161 cagtacgatg taaatcatgg caatcacttt aaatttcggg atgcaggatg cgcaaagtgc
     5221 gggtactgcg gttaaatcgg gcttgcgttt ccggcagtct gacggaacgg ccggttataa
     5281 cgtttgaatt ataacgaaaa ttgcagggtc tgacagcagt gtgtcgaaat aagcggaaat
     5341 tttccgaaat gccgtctgaa atctgtggtt ttcagacagc atttctgttt acgggaaacc
     5401 ctttcccccg tatccgccgc cagtcgaaaa aatggccggg gtcggttttg cggccgggtg
     5461 cgatgtcttg gtgtcctgtt accgccgtga tggggtagcg gcggcagatt gcgtccaaca
     5521 gcgtttcgag cgaacggtat tgcgcttcgg taaagggttc gaaatcgcag ccttccaatt
     5581 cgatgccgat ggaaaatgca ttgcattttt ccctgccgcc gaatgaagac acgccggcgt
     5641 ggtatgccat attgtcgcag gaaacgaact gtaccgtttc gccgtcgcgt ttgattaaaa
     5701 aatggctgga tacgcgcaaa gtgtgtatca ggctgaagaa cggatgtccg tcggggtcga
     5761 gccggttggc aaacagcttt tccaccgcat ccgtaccata ttcgaacggc ggcagcgaga
     5821 tattgtgcaa tacgacaagg gaaacctctt ctttggactc cctcgggccg aaattcggcg
     5881 acggaatgtg gcgcacgctt tgcagccaac cgtcctgcca gggtgccgta gcgggattgt
     5941 ccatgatgtc ctttctgtct tatggggtaa tttgggttat actgtcgccc gaattttaag
     6001 acgtattccg aatgctggga atcctaccat gttgagaaaa ttgttgaaat ggtctgccgt
     6061 ttttttgacc gtgtcggcag ccgttttcgc cgcgctgctt tttgttccta aggataatgg
     6121 cagggcatac cgaatcaaga ttgccaaaaa tcagggtatt tcgtcggtcg gcaggaaact
     6181 tgccgaagac cgcatcgtgt tcagcaggca tgttttgacg gcggcggcct acgttttggg
     6241 tgtgcacaac aggctgcata cggggacgta cagactgcct tcggaagtgt ctgcttggga
     6301 tatcttgcag aaaatgcgcg gcggcaggcc ggattccgtt accgtgcaga ttatcgaagg
     6361 ttcgcgtttt tcgcatatga ggaaagtcat cgacgcaacg cccgacatcg aacacgacac
     6421 caaaggctgg agcaatgaaa aactgatggc ggaagttgcc cctgatgcct tcagcggcaa
     6481 tcctgaaggg cagtttttcc ccgacagcta cgaaatcgat gcgggcggca gcgatttacg
     6541 gatttaccaa atcgcctaca aggcgatgca acgccgactg aatgaggcat gggaaagcag
     6601 gcaggacggg ctgccttata aaaaccctta tgaaatgctg attatggcga gcctgatcga
     6661 aaaggaaaca gggcatgaag ccgaccgcga ccatgtcgct tccgtcttcg tcaaccgcct
     6721 gaaaatcggt atgcgcctgc aaaccgaccc gtccgtgatt tacggcatgg gtgcggcata
     6781 caagggcaaa atccgtaaag ccgacctgcg ccgcgacacg ccgtacaaca cctacacgcg
     6841 cggcggtctg ccgccaaccc cgatcgcgct gcccggcaag gcggcactcg atgccgccgc
     6901 ccatccgtcc ggtgaaaaat acctgtattt cgtgtccaaa atggacggta cgggcttgag
     6961 ccagttcagc catgatttga ccgaacacaa cgccgccgtt cgcaaatata ttttgaaaaa
     7021 ataaaccatg ccgtctgaaa agtttgtgtt ttcggacggc atacccttac cggaactgca
     7081 agcatgaaac cgcaattcat cactttggac ggcatagacg gtgccggcaa atccaccaac
     7141 cttgccgtca tcaaggcatg gtttgaacgg agggggctgc ccgtgctgtt cacgcgcgaa
     7201 ccgggcggaa cgccggtcgg tgaggccttg cgcgaaatcc tgctcaaccc tgaaaccaaa
     7261 gccggtttgc gtgcggaaac actgatgatg ttcgccgcca gaatgcagca tattgaagac
     7321 gtgatattgc ctgcactttc agacggcatc catgtcgtgt ccgaccgttt taccgacgca
     7381 accttcgcct atcagggcgg cgggcggggg atgccgtctg aagacattga aattttggaa
     7441 cattgggtgc agggcggttt gcgccccgat ttgaccctgc tgttggatgt gccgctggaa
     7501 gtgtcgatgg cgcgtatcgg acagtcgcgc gagaaagacc ggttcgagca ggaacaggca
     7561 gatttcttca tgcgtgtgcg cagcgtttat ctgaaccgtg ccgccgcctg tcccgaacgg
     7621 tacgccatta tcgacagtaa ccgcagcttg gatgaagtca gaaacaacat agaaaaagtg
     7681 ttggacggac atttcggctg ttaatgcggc aaatattgaa acaagcgcat ccacccgcgc
     7741 cgaaaatcaa acggcagtgc cgcaggtgaa aatggcggta tgcgccaaac tttcggcatg
     7801 atagaattac gctcggttac aaggcaggat gcgtcggcaa tattaacgaa ccgcccgtaa
     7861 catgatgacc cgaaagcgtt tcggacagtc cgattcaaat ctttttctcg caacaggatt
     7921 gacacatgga aaactcattg aaagaagccg ccctcaagtt ccacgaatta cccgttccgg
     7981 gcaaaatttc cgttaccccg accaaatctt tggcgaccga caaagatttg gcgttggcgt
     8041 actctccggg cgtagccgct ccttgtatgg aaatccatgc cgatccgcaa aatgcctaca
     8101 aatacaccgc caaaggcaac ttggtcgccg tcatttccaa cggtacggcc gttttgggct
     8161 tgggcgacat cggcgcgctg gcgggcaaac ccgtgatgga aggcaaaggc gtattgttca
     8221 aaaaattcgc cggtgtggac gtgttcgaca tcgaaatcga tgaaaaagac ccgcaaaaac
     8281 tcgtggacat catcgccgct ttagagccga ccttcggcgg catcaacctc gaagacatca
     8341 aagcacccga gtgtttctac atcgaacgcg aattacgcaa acgctgcaaa atccccgtat
     8401 tccacgacga ccagcacggc acggccatca ttaccgccgc tgccgtattg aacgccctgc
     8461 gttttaccgg ccgtaaaatc gaagaagcga ctttggtgtg ttccggcgca ggtgcggccg
     8521 cgattgcctg cttgaaccaa ttgctggatt tgggcttgaa acgcgaaaac gtgaccgttt
     8581 gcgactccaa aggcgtgatt taccaaaccc gcgaagacaa agatcgcatg gacgaatcca
     8641 aaaaattcta cgccgttgaa gacaatggcc agcgcgtact tgccgatgcc gtcaaaggta
     8701 aagacatctt cttgggcctc tccggcgcga acctgctgac gcctgaaatg ctgaacacca
     8761 tgaacgaaaa acccatcgtg ttcgccatgg ccaacccgaa tccggaaatt ctgccgccgc
     8821 tggcaaaaga aacccgtccg gacgtggtta tcggtaccgg ccgttccgac ttcccgaacc
     8881 aagtcaacaa tgtattgtgc ttcccgttca tcttccgtgg cgcgttggat gtcggcgcga
     8941 ccaccatcaa cgaagaaatg aaacgcgcct gcgtgtatgc tttggcggat ttggcgatgg
     9001 aagaagtgac cgaagaagtg gttgccgctt acggtaagaa atttgaattc ggcgcggaat
     9061 acctgattcc gactccgttc gattcccgcc tgctgccgcg cgtcgctacg gctgccgcca
     9121 aagcagcgat ggaaagcggc gttgcaaccc gtccgattgc cgactttgaa gcctacgccg
     9181 ccaagctggg cgaatggaag ctgtaagccg tttgcggttt aaaatgccgt ctgaactgtt
     9241 ttcaggcggc attttgctgt cagattgata aatgaaagat actggaaaat gaaagagatg
     9301 aaacctgtcc gttatcatat tggcgatatg cccgagactt caaaacaaac cgcctcccgt
     9361 catgacgaca gggcagtggg tgttgacgat gattgttttc atgcttcctt tggtcaattt
     9421 ttgtttgggt gttcggcaga ggcaacccga accacgccaa tttctgtaaa gcgcagttgc
     9481 ttatttacct gattggttcg cttatcggtt tggtcttcgc gttgtttata ggtgggtctg
     9541 tatcgggtac gcatgattaa tgccccgggc tgattttgcc tcgaggattt gtatcgaata
     9601 tgccgaattg tttcaaattt cataccgtta tcgaacggca ttggcaaaaa ccttatccgg
     9661 ttttgtcttt tctgcttaag ccgctctccg ggctgtttgc caaaattgcg gcaaaacggc
     9721 ggacggattt tttatcggga aaacggcaaa gcgaaaagct gcccgtgcct gtggtcgtgg
     9781 tcggcaacat ccatgccggc ggtacaggaa aaacgccgat tgttgccgcg ctggtgtcgg
     9841 gtttgcagga aaaaggtgtc aaggtcggca tcatcagccg gggctacggg cgcaagagca
     9901 aggcggttca tgtattgaat gctgagagcc gagcggaaga tgcgggcgat gagcctttgc
     9961 tgctgttccg caaaaccggt gcgccgacgg cggtgggcag cagccgtgca gaggcaggca
    10021 gggcgttgct ggcggcgcat cccgacatcg gactgattgt ggcggacgac ggtttgcagc
    10081 attacgccct gcggcgagat gtggaaatcg cggtgtttcc ggcggcggat acggggcgca
    10141 cggatttgga tttactgccc aacggcagtt tgcgcgaacc tttgttgcgg ctggattcgg
    10201 tggatgcggt cgtcgtcagc ggcggcaagg cggatgcgct gtttaggccg tctgaaaata
    10261 tgtttcacag ccgtatcgaa gcgggacgga tttaccgttt gaacaatccg tccgaaatac
    10321 tggacacagg ccgtctgaaa aatcaaaccg tcgtcgccgt ggcaggtatt gccaagccgg
    10381 cgcgtttttt cgattcgttg cggaatatgg gcattaccgt gaagcgaacc gtcgcgctgc
    10441 ctgaccacgc cgacatttcg gcggcagatt tgcccgatgc ggacgcggtc attattacgg
    10501 agaaagatgc ggtcaaattt tcagacggca tttgcaccga taatgtttgg gtgttgcccg
    10561 tttgtgcgat aatcgaacct gatttggcgg cgtttgtgtt ggagcggttg gaagatgtac
    10621 cgaaggccgt ctgaaagcac ggtttgggcg gagtgattac ggatttgaat aagaacgcct
    10681 cgcgccgtca ttcccgcgca ggcgggaatc taagtctcga attttcagga atgcctagga
    10741 ggcttcagaa atcccaaatc tccggatttc cacttggaca ggaatgagaa aaccggtcgt
    10801 attttttatc tgcattaatc attcattaaa ggattgaata ttaaactgaa aaccttgtta
    10861 ttgcccttcg ccacgctggc attgtgcacc aatgcttttg ccgctccgcc cagcgacgcg
    10921 tcgttggcga gttggctgga tacgcagaat tttgaccggg atatagaaaa aaatatgatt
    10981 gagggcttta atgccggatt taaaccgtat gcggacaaag cccttgccga aatgccggaa
    11041 gcgaaaaaag atcaggcggc aaaagccttt aaccgctatc gtgagaatgt tttgaaagat
    11101 ttgattacgc ccgaagtgaa acaggctgtc cgtaatactt tattgaagaa tgcccgtgag
    11161 atatacacgc aagaagaaat tgacggcatg attgcctttt acggttcgcc tgtcggtcag
    11221 tccgtcgttg ccaaaaatcc gcgcttaatc aagaaatcga tgagtgaaat agcagtatct
    11281 tggactgcat tgtcagggaa aatcgcgcga catcatctgc ccgcgtttac ggaagagttg
    11341 cggcgcatca tctgcggtgg taaaaatccc gatgcgggct gtaaacaagc cggacaggtt
    11401 gggaaaaggc atcagaaata aatgatagcc gtctgaaata ttgaagaggg catccgattg
    11461 attgaaccat caaacccgaa agcaacccta tggaaaaaaa attcttagac atcctcgtct
    11521 gccccgttac caaaggcagg ctggaatatc atcaggacaa acaggaattg tggagccgtc
    11581 aggcgaagct tgcctatccg attaaagacg gcattcccta tatgctggaa aacgaagcgc
    11641 gaacgttgag cgaagaggaa ctcaaagcat gaccgaattc gtcgtattga ttccggcgcg
    11701 gctggattcg tcgcgcctgc ccagaaaagc cttggcggac atccacggca aaccgatggt
    11761 cgtgcgcgtt gccgaacagg cggcaaaaag taaagctgcg cgcgtcgttg ttgccaccga
    11821 ccatcccgat attcagacgg tctgtcaggc gcacggcgtc gaagtcgtca tgacttcaaa
    11881 ccggcacgaa agcggcacga cgcgccttgc cgaagcctct gccgcgctga agctgccgcc
    11941 gcatctggtg gttgtcaacg tacagggcga cgagccgctg attgcccccg aactcatcga
    12001 ccgcactgcc gaagtcttgg tggaaaacaa cgtacagatg gcaactgcgg cacacgaatt
    12061 gcacgatttc gacgaattga tgaatcccaa tgccgtcaaa gtcgtcctcg acaaaaaccg
    12121 caacgccatc tacttcagcc gcgccccgat tccctatccg cgcgatgcga tgcgtgccgg
    12181 aaaacgcgaa atgccgtctg aaaccgccgt cctgcgccat atcggcatct acgcttaccg
    12241 cgccggcttc ctgcaacgct atgccgaaat gagcgtttcg ccgctggaaa ccatcgaatc
    12301 gctggaacag ctgcgcgtcc tgtggcacgg ttatcccatt gccgtcgaaa ccgccaaaga
    12361 agcccccgcc gccggtgtgg atacgcagga agatttggat agggttcgcg ctgtatttca
    12421 gaccgtataa aacaggttca aagggaaaag atatgcagca acatattgaa aagtggcaac
    12481 acttgagccg ggaagaacag aaaatccttg ccgaagtatg gggtctcgtg caaaacgatg
    12541 atcaggaggt tcactatgaa atgcttaaat tgaacgcacc cgacgaagcc agtggcgaat
    12601 tttggttcag aatggcagaa acactcagca ccctgccgcc caaccgttcc ctcgacctta
    12661 gaatgaacgg cggcaggctg gcgaccgccg tatccatcct ttccgtcatg attgaagaca
    12721 atcccgacat accgcagctt tgggcgcaaa aaattaccgc gctcaattat agtggattaa
    12781 atttaaacca gtacggcgtt gcctcgcctt gtcgtactat ctgtactgtc tgcggcttcg
    12841 tcgccttgtc ctgatttttg ttaatccact atatttggca cacggacaca aagcccgtgc
    12901 cgacggtttg gcacaacagc ccgacaaagc ggcagaagcc aacgaggagg aatacctgac
    12961 caaagccctg tcgcaaaacc tgctgtcaac attggatgtc gcgcttgcac gttttcctga
    13021 agacgcgtgg tttcaggaaa tcaaacagga tgcacaaaag cattttgctt gaggatgtgg
    13081 cagtcaggaa tatttccatt caggaagaaa agaagtgcct gattgggtat aatcagggta
    13141 aatcttattt tatttcaaaa gattaatatt tgctttctgt ttttccttga cggtatcgga
    13201 aaagttgatt atagttacag cttccttagg agtaatggct gagaggctga aggcacttcc
    13261 ctgctaagga agcatgtggg gtcaacctgc atcgagggtt cgaatccctc ttactccgcc
    13321 agataaaaaa tagacgctgt gttttacagc gtctattttt tatgcaattt tatagcgggt
    13381 tggtgcaaaa ccagtatggt attgccgtgt cttgattctg aatttt
//
LOCUS       DAWHSK010000027        13394 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.027, whole genome shotgun sequence.
ACCESSION   DAWHSK010000027 DAWHSK010000000
VERSION     DAWHSK010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13394
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.027"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(110..538)
                     /locus_tag="WIC03_000552"
     CDS             complement(110..538)
                     /locus_tag="WIC03_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family phosphatase"
                     /protein_id="HEZ3883097.1"
                     /translation="MAILKLDEHLYISPQLTKADAEQIAQLGIKTVICNRPDREEESQ
                     PDFAQIKQWLEQAGVTGFHHQPVTARDIQKHDVETFRQLIGQAESPVLAYCRTGTRCS
                     LLWGFRRAAEGMPVDEIIRRAQAAGVNLENFRERLDNARV"
     gene            complement(603..2351)
                     /locus_tag="WIC03_000553"
     CDS             complement(603..2351)
                     /locus_tag="WIC03_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family metallopeptidase"
                     /protein_id="HEZ3883098.1"
                     /translation="MIHYKITPSPLDHEWHILLTFTQDDDLPIEISLPNWVPGSYLIR
                     DFSRHITSIHASCNGTSMPLEQIAKNRWHAAAVRGEWQIRYTVYAFDLSVRGSFLTTE
                     RGFFDGSCLFLKVEGTETLPHRLELTGIPSEWRIATTLPETGRFVFQAASYAELIDRP
                     VEMGLIEFLDFEAAGIPHTIALSGIYPDFDRNRLVSDIKKICETELAVFSSPAPFQKY
                     LFLLHVGDHIYGGLEHTDSTALLADRHSLPPYGMTDADDTYTTLLGLFSHEYFHAWNV
                     KSIKPAAFVPYDLDKENYTEQLWAFEGITSYYDDLFLARSRTISPESYLNLLAQGITR
                     VQQTRGRLRQTLAESSFTAWNKFYKPDENSPNAIVSYYQKGALAALCLDLIIRNRSNG
                     RHSLDTVMDKLYREWRDTHSGIPEKHWQIRCQEITGLDLEDFFQKTLYSTEDLPLAEC
                     LATAGVGLTFLPLPRQHGGGYAEHICPVPSAGDFGARFKQNTDHIVLTHVFNGGSAES
                     AALCPQDKIIALDGYACTDFTAQWARYHVNAKINIHFFRAGILRQTVLTVQAAAADTA
                     ILHITDRNLLDNWLFG"
     gene            2678..4111
                     /gene="ccoN"
                     /locus_tag="WIC03_000554"
     CDS             2678..4111
                     /gene="ccoN"
                     /locus_tag="WIC03_000554"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HEZ3883099.1"
                     /translation="MDTQTYNYKVVRQFAIMTVVWGIVGMLVGVIVAAQLFAPALDLS
                     NIGPWFHFGRLRPLHTNAVIFAFGGCGLIGTSYYVVQRTCNTRLFGGWLPAFTFWGWQ
                     AVIVAAVVSFPMGWTQGKEYAELEWPIDILITLVWVAYAIVFFGTIAKRKIKHIYVAN
                     WFYGGFILAVALLHIVNNISIPAGLMKSYPVYSGAIDAMVQWWYGHNAVGFFLTAGFL
                     GMMYYFVPKQAARPVYSYRLSVVHFWALIFTYMWAGPHHLHYTALPDWTQSLGMVLSL
                     ILFAPSWGGMINGIMTLSGAWDKLRTDPILKFLIVSLSFYGMSTFEGPMMSIKTVNAL
                     SHYTDWTVAHVHAGALGWVGFVTIGSVYYMIPRLFGKEQMHSTKLVEAHFWIATIGVV
                     LYIAAMWIAGVMQGLMWSSLNDDGTLTYSFVESVKRTMPYYVIRFAGGLLYLSGMCIM
                     AYNVYRTAIGGKAVDAEIPAVSQTQHH"
     gene            4138..4749
                     /gene="ccoO"
                     /locus_tag="WIC03_000555"
     CDS             4138..4749
                     /gene="ccoO"
                     /locus_tag="WIC03_000555"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HEZ3883100.1"
                     /translation="MKLQQLAEEKIGVLIVFTLLVVSVGLLIEVVPLAFTKAATQPAP
                     GVKPYNALQVAGRDIYIREGCYNCHSQMIRPFRAETERYGHYSVAGESVYDHPFQWGS
                     KRTGPDLARVGGRYSDEWHRIHLLNPRDVVPESNMPAFPWLARNKVDVDATVANMKAL
                     RKVGTPYSDEEIAKAPEALANKSELDAVVAYLQGLGLALKNVR"
     gene            4754..4924
                     /locus_tag="WIC03_000556"
     CDS             4754..4924
                     /locus_tag="WIC03_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcoQ/FixQ family Cbb3-type cytochrome c oxidase
                     assembly chaperone"
                     /protein_id="HEZ3883101.1"
                     /translation="MDINGIRALFTVWIFICFLLVLYIVFNRRNKKNYDNAANSIFDE
                     NQDAQDKKSENR"
     gene            4948..6045
                     /gene="ccoP"
                     /locus_tag="WIC03_000557"
     CDS             4948..6045
                     /gene="ccoP"
                     /locus_tag="WIC03_000557"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HEZ3883102.1"
                     /translation="MNTTSQFTSNFWNIYIAVIVLLSFIALAWLLLSQNVVKRPKKGE
                     EVQTTGHEWDGIAEYDNPLPRWWFWLCVLTWLFGIGYLVMYPGVGDYKGLLKWTSHNQ
                     YEKEVKKADEQYGKLYAKFADMPIEKVAKDPQAKQIAQNLFNTYCIQCHGSDAKGSKG
                     FPNLTDSDWLWGGDPDKIHETIEKGRVATMPAWGPALGEEGVKDVAHYVMSLSKPEGQ
                     YDEERAARGQALFSGPPANCFTCHGDKGQGIQGLGPNLTDDVWLWGGTQKSIIETITN
                     GRSSQMPAWGHFLDKDKLHIMTAYVWGLSDKDGKALVKKAEPAPTPAPAAEPAASAPA
                     EAAQAASEAKPAAAEPKAEEKAAPAAKADGK"
     gene            6158..6283
                     /locus_tag="WIC03_000558"
     CDS             6158..6283
                     /locus_tag="WIC03_000558"
                     /inference="COORDINATES: protein motif:HMM:NF024834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
                     /protein_id="HEZ3883103.1"
                     /translation="MHKHAIEGFNTMPAKGGRGDLSDDEVKAAVDYMVNQSGGKF"
     gene            6485..7894
                     /locus_tag="WIC03_000559"
     CDS             6485..7894
                     /locus_tag="WIC03_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEZ3883104.1"
                     /translation="MDTQIKTEADNQSNRRYLTVWRWHFYAGLLVMPFLTLLAVTGLG
                     MLLFANITGKEGERIHVVPQATVQPLSVQAEAARSAVNPETSSVVQYIAPRADDMVAV
                     FRVNNEGKATMVAVDPYTAKVVSTMPRNQGWYYAMDEIHSDMMLGSTGDYLLETAASL
                     TIIMIISGLYLWWVKRRGIKAMLLPPKGRARSWWRNLHGAFGTWVSLILLLFCLSGIA
                     WAGIWGGKFVQAWSQFPAGKWGVEPNPVSVVPTHGEVLNDGKVKEVPWVLELTPMPVS
                     GTTVGKDGINPDEPMTLETVDRFAREIGFKGRYQLNLPKGEDGVWTLSQDSMSYDMIS
                     PFADRTVHIDQYSGKILADIRFDDYNPFGKFMAASIALHMGTLGWWSVLANVLFCLAV
                     IFIGISGCVMWWKRRPTGAVGIVPPAQKVKLPVWWMMALPLLAIALLFPTALLAIAVI
                     WLLDTLLLSRIPVLRRWFK"
     gene            7996..11205
                     /gene="recC"
                     /locus_tag="WIC03_000560"
     CDS             7996..11205
                     /gene="recC"
                     /locus_tag="WIC03_000560"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HEZ3883105.1"
                     /translation="MFYLYQSNRLETLAALFARIQKVKPLKSVLQPEQIIVQSQGMRR
                     YLNTCLARDLGVAANLSFSLPAGLTWKLMKKLIPGIPELSPFAPEVMRWRLLDLFRSE
                     AFRNTAEFEDVRNVLQDYLGSGESADYQLAGQLADIFDQYLVYRPQWIDAWQQGRILS
                     LGDDEIWQSKLWRYLDDGRQSAPHRVALWEKLLESLSSDKLPERYFVFGISTMAPMYL
                     QLLHKLSEHCDVFVFALNPSGMYWGNVIEAAQILKGGGDPDLTQAGHPLLASLGKQGR
                     DFFDFLNEMEIEGETPVFEEGGRDTLLHALQTDIQNLKMPSENVGSVGTDDGSIRIVS
                     AHSPLRELQILKDKLLKILHEHPDWQPHDIAVLTPNIESYTPFIEAVFGQAQPGAQAL
                     PYSVSDVKISRRQPLFHALSCLFDLLESRFEVDKVLVLLETAPVLRRFGLTEDDLPLL
                     HDMVADLNVRWGLDGEMRGGTDQLFTWKQAVERMILGWMLPEGGNPMWQDVSAWYADV
                     NQTAMFGRFAAFLETLSDIVRIWRQPATVDEWVGRCRGLLETLFQAEADDQKAVQNLE
                     NEWVKWQAETELAQFSGQLPQQTVIRHIRRFLDSESEAGFLRGGITFCSMVPMRSLPF
                     KVICLLGLNDGDFPRNTKAAVFDLIAKHPAKGDRARRDDDRYLFLEALISAREILYLS
                     YIGRDIRKDEELAPSSLLGELIDTVAAMTGTNSRQLAQNWIEQHPLQAFSRRYFQEGG
                     RSDGIFGTRTDYATALGQTPEPPQPFFDQPVENAEPVAEIGQDEFIRFWRNPVKVWLQ
                     QQLAWSEPHIGEAWEPAEPFEPQHADQIAETYIEARREGRDFAQTAARIGAESLLPSG
                     ELGRLWQQNFQTAAKQIDTAVLNSPKLPPLSYAIPSDGQILKGSLGNLYRCGQVFYAY
                     GKPNAPQRIAFLLEHLIFCAVMPSEAETRQTFIVQSGETEILAEIAQDRALQLLSEWM
                     AFFNIGQNRPLPFFAKTSLAAAEAFAQKQDWEAALKKAQTAYYGNKVSKGQKDYTEVA
                     LVFGNASQNPLEQPLFENLARLLADTLAAAEKREGAGAA"
     gene            complement(11275..12843)
                     /gene="mtrF"
                     /locus_tag="WIC03_000561"
     CDS             complement(11275..12843)
                     /gene="mtrF"
                     /locus_tag="WIC03_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041421321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family antimetabolite efflux transporter
                     MtrF"
                     /protein_id="HEZ3883106.1"
                     /translation="MSQTDTQRDGRFLRTVEWLGNMLPHPVTLFIIFIVLLLIASAAG
                     AYFGLSVPDPRPVGAKGRADDGLIHVVSLLDADGLIKILTHTVKNFTGFAPLGTVLVS
                     LLGVGIAEKSGLISALMRLLLTKSPRKLTTFMVVFTGILSNTASELGYVVLIPLSAII
                     FHSLGRHPLAGLAAAFAGVSGGYSANLFLGTIDPLLAGITQQAAQIIHPDYVVGPEAN
                     WFFMVASTFVIALIGYFVTEKIVEPQLGPYQSDLSQEEKDIRHSNEITPLEYKGLIWA
                     GVVFVALSALLAWSIVPADGILRHPETGLVSGSPFLKSIVVFIFLLFALPGIVYGRVT
                     RSLRGEQEVVNAMAESMSTLGLYLVIIFFAAQFVAFFNWTNIGQYIAVKGATFLKEVG
                     LGGSVLFIGFILICAFINLMIGSASAQWAVTAPIFVPMLMLAGYAPEVIQAAYRIGDS
                     VTNIITPMMSYFGLIMATVIKYKKDAGVGTLISMMLPYSAFFLIAWIALFCIWVFVLG
                     LPVGPGAPTLYPAP"
     gene            13177..13389
                     /locus_tag="WIC03_000562"
     CDS             13177..13389
                     /locus_tag="WIC03_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883107.1"
                     /translation="MKNEIQKIMDKYDPWHEDDFESYEDIAKDVSLMTDKTFIEHYLL
                     EVYSEENGHFDQENVHAMIEEIKNAI"
BASE COUNT         3354 a         3195 c         3563 g         3282 t
ORIGIN      
        1 actgtaaaaa cggagaagga cagaggacgg cagtaaggtt tgccgtcctc tttttcaagt
       61 tgcggtggaa tgccgcttga aggtgtggtt taaacgtttc ggcttgtaat cagacgcggg
      121 cgttgtccag ccgctctctg aagttttcca aatttacgcc tgccgcttgg gcgcggcgga
      181 tgatttcgtc aaccggcata ccttctgccg cccggcggaa gccccacagg agggagcagc
      241 gcgtaccggt ccggcaatag gcaaggacgg gagattcggc ttgtccgatg agttggcgga
      301 aggtttcgac atcgtgtttt tggatgtcgc gtgcggtaac gggttggtga tggaatccag
      361 taacgcctgc ttgttccagc cactgtttga tttgggcgaa gtcgggttgc gattcttctt
      421 cgcggtcggg gcggttgcag atgacggttt tgatgcccag ttgcgcgatt tgttccgcat
      481 cggctttggt cagttgcgga gaaatataga ggtgttcgtc aagtttcaga atggccattt
      541 tgtttccttt actttgcggt tgtttttcag acggcatttt gtgtgcaatg ccgtctgaaa
      601 gtttaaccga acaaccagtt gtccaacagg tttcggtctg tgatatgtag gatggcagta
      661 tccgctgccg ctgcctgaac cgtcaagacg gtttgacgca atatgccggc acggaagaag
      721 tggatattga tttttgcatt gacgtggtat cgggcccatt gtgcggtaaa gtcggtgcag
      781 gcataaccgt ctaaagcaat gattttgtct tgcgggcaca gtgccgcaga ttccgcgctg
      841 ccgccgttga agacatgggt caggacgatg tggtcggtgt tttgtttgaa acgtgcgcca
      901 aaatcgcctg ccgacgggac ggggcagatg tgttctgcgt atccgccgcc gtgttgtcgg
      961 ggaagcggca ggaaggtcag tcccacgcct gcggttgcca ggcattcggc aagcggcaaa
     1021 tcttcggtac tgtataacgt tttttggaaa aaatcttcca aatccaagcc ggtaatttcc
     1081 tgacagcgga tttgccagtg tttttccgga atacccgagt gtgtgtccct ccactcccga
     1141 tagagtttgt ccattaccgt atcgagagaa tgtctgccgt tgcttcggtt gcgtattatc
     1201 agatcaaggc acaatgcggc aagcgcgcct ttctggtagt agctgacgat ggcgttgggg
     1261 ctgttttcat caggtttgta aaatttgttc cacgcggtaa aactcgattc cgccaaggtc
     1321 tgcctcaaac ggccgcgggt ttgttgtacg cgcgtaatgc cttgtgccag caggtttaaa
     1381 taagattcgg gcgagatggt gcggctgcgt gccaaaaaca aatcgtcgta ataggatgta
     1441 atgccttcga atgcccatag ttgttcggta tagttttctt tgtcgaggtc ataagggacg
     1501 aacgcggcag gtttgatgga tttgacgttc cacgcgtgaa aatattcgtg ggagaaaagt
     1561 ccgagcaatg tggtgtaggt atcgtcggca tcggtcatac cgtacggcgg aaggctgtgg
     1621 cggtcggcga gcagggcggt gctgtcggtg tgttccaaac cgccgtaaat atggtcgccg
     1681 acgtggagca ggaacaaata tttttgaaac ggggcagggg aggaaaacac cgccagttct
     1741 gtttcgcaga tttttttgat atccgaaacc agcctgttgc ggtcgaaatc gggatatatg
     1801 ccgcttaagg caattgtgtg cggaatgcct gccgcctcaa aatctaaaaa ttcaatcaag
     1861 cccatctcga caggtcggtc aatcaattcg gcataagatg ccgcctgaaa gacaaacctc
     1921 cctgtttccg gcagcgttgt ggcaatacgc cattcggacg gaatacccgt caattccaag
     1981 cggtgcggca gcgtttccgt tccttcgact ttcaaaaaca ggcacgatcc gtcaaaaaaa
     2041 ccgcgttctg tcgtcaggaa agaacctcga accgacaaat cgaatgcata tacggtgtag
     2101 cggatttgcc actcgccgcg tacggcggcg gcatgccagc ggtttttggc aatttgttcg
     2161 agcggcatgg acgtgccgtt acaggatgca tggatagaag tgatgtggcg ggaaaaatcc
     2221 cgaatcagat agctgcccgg aacccagttt ggcaggctta tttctatagg aagatcatca
     2281 tcttgtgtga atgtcagcag gatgtgccac tcgtggtcga gaggggacgg ggttatttta
     2341 taatgaatca taactatcaa atatatcgtt aaacgggatg gattaaatac ctttaaaatg
     2401 cagcttaacc tacatcaata aagtgccaca aaaagggaaa attggaaaca aaagcaatgg
     2461 cggtcgggtt gtaatttatt ataacaatat gatttatatg taataaaaaa cagattaagc
     2521 aaagggctcg ccggcggcgc gaatgtaatt ccgcttggcg taataaggca gatatgttaa
     2581 atttagaaaa aattacgctt gcccggattg caccttaaac aggctgcgga agcggcagga
     2641 taaaaatctt tattcccata aattcatgga gactctcatg gacacacaaa cttacaacta
     2701 caaagtggtg cgccagttcg ccatcatgac tgtagtttgg gggattgtgg gtatgttggt
     2761 cggcgttatc gtcgccgccc agctttttgc tcctgccctc gacttgtcta atatcggacc
     2821 ttggttccac ttcggccgcc tgcgtccgct gcacaccaat gcggttattt tcgcatttgg
     2881 cggttgcggc ctgatcggca catcatacta cgttgtccaa cgtacttgta atacccgtct
     2941 tttcggcggt tggctgccgg catttacctt ttggggctgg caggcggtaa tcgttgccgc
     3001 cgtcgtcagc ttccctatgg gttggaccca aggtaaggaa tatgccgaac tggaatggcc
     3061 gatcgatatt ctgattactt tggtttgggt ggcttacgcc atcgtattct tcggtacgat
     3121 tgccaaacgt aagattaaac atatttacgt tgccaactgg ttctacggcg gctttatttt
     3181 ggcggtcgca cttttacaca tcgtcaacaa tatcagcatc ccggccggtt tgatgaagtc
     3241 ataccccgtc tattccggtg cgattgatgc tatggttcaa tggtggtacg ggcataatgc
     3301 cgtgggcttc ttcctgactg ccggcttctt gggtatgatg tactatttcg tacccaaaca
     3361 agcagcccgc cccgtttact cctaccgcct gtccgtcgtt cacttctggg cgttgatttt
     3421 cacctatatg tgggcgggtc cgcaccatct tcactacact gcgctgcctg actggacgca
     3481 atctttgggt atggttctgt ctttgattct gttcgcaccc tcttggggcg gtatgattaa
     3541 cggcatcatg accttgtccg gcgcgtggga caaactgcgt acagacccga ttcttaaatt
     3601 cctgattgta tccttgtcct tctacggtat gtctaccttt gaaggcccga tgatgtcgat
     3661 taaaacggtc aatgcattga gccactatac ggactggacc gtcgcgcacg ttcatgcggg
     3721 tgcgttgggc tgggtaggct ttgtaaccat cggttccgtc tattacatga ttccccgtct
     3781 gttcggcaaa gaacagatgc acagcaccaa gctggtagaa gcacattttt ggattgcgac
     3841 catcggcgtg gttctgtata tcgctgccat gtggattgcc ggtgtgatgc agggtttgat
     3901 gtggagttct ttgaacgatg acggtacgct gacctactcg tttgtcgaat ccgtaaaacg
     3961 caccatgcct tactacgtga tccgttttgc aggtggttta ttgtatctga gtggtatgtg
     4021 cattatggcg tacaacgtgt accgcacagc catcggtggt aaagcagtcg atgccgaaat
     4081 ccctgcggtt tcccaaacac agcaccacta aaatactaag aaagataggc taccaaaatg
     4141 aaattacaac aattggctga agaaaaaatc ggcgttctga ttgtgttcac gctgcttgta
     4201 gtcagtgtcg gtctgttgat tgaagttgtg cccttggcct ttaccaaggc ggcaacacag
     4261 ccggcaccgg gcgtgaagcc ttacaatgcc ttgcaggttg ccggacgcga tatttacatc
     4321 cgtgagggct gttacaactg ccactctcaa atgattcgtc cgttccgtgc ggaaaccgag
     4381 cgttacggtc attactctgt tgccggagag tcggtttacg accatccttt ccaatggggt
     4441 tccaaacgta ccggtcctga tttggcacgt gtgggcggcc gctattccga cgaatggcac
     4501 cgtatccacc tgctgaatcc ccgtgatgtc gtgcctgagt ccaatatgcc ggcattccca
     4561 tggcttgcac gcaataaagt cgatgtcgat gcaaccgttg ccaacatgaa ggctttgcgt
     4621 aaagtaggta ctccttacag tgatgaggaa attgcgaaag cgcctgaggc tttggcaaac
     4681 aaatccgagc tggatgctgt agtcgcctat ctgcaaggat tgggtctggc tttgaaaaac
     4741 gtaaggtaac atcatggata ttaacggtat tcgcgctctt ttcacggtat ggatctttat
     4801 ctgtttcctg ttggtactct atatcgtctt caacaggcgg aataagaaaa actacgataa
     4861 tgccgcaaac agcatttttg atgaaaacca agatgcgcaa gataagaaaa gcgaaaaccg
     4921 ttaatattgt gataacggag caaaacaatg aacacaacat cccaatttac cagtaatttc
     4981 tggaatatat atattgcagt tattgtctta ctgagcttta tcgctttggc ttggctgctg
     5041 ctgtctcaaa atgttgtcaa acgtccgaag aagggcgaag aagtacaaac tacgggtcat
     5101 gagtgggacg gcattgccga atacgacaat ccgctgcccc gctggtggtt ttggctgtgt
     5161 gttttgacgt ggctgttcgg tatcggttat ttggttatgt atccgggtgt cggcgactac
     5221 aaaggtctgc tgaaatggac cagccataac caatatgaaa aagaggtcaa aaaagccgat
     5281 gagcaatacg gcaaactgta tgccaagttt gcggatatgc cgattgaaaa agtggcaaaa
     5341 gaccctcaag ccaagcaaat cgcccaaaac ctgtttaaca cttattgtat ccagtgccac
     5401 ggctctgatg ctaaaggctc taaaggtttt ccgaatctga ccgatagcga ttggttgtgg
     5461 ggcggtgatc ccgataaaat tcacgagacc atcgaaaaag gccgtgttgc gactatgcct
     5521 gcctggggcc ctgctttggg cgaagaaggc gtgaaagacg ttgcccatta tgtgatgtcc
     5581 ctttcaaaac ccgaaggtca gtatgatgag gaacgtgccg cgcgcggaca agccttgttc
     5641 agcggtccgc ctgccaactg tttcacttgc cacggcgata agggacaagg tatccaaggt
     5701 ttgggtccga acctgactga tgacgtgtgg ttgtggggcg gtacgcaaaa atccattatc
     5761 gaaaccatta ccaacggtcg cagcagccaa atgcccgctt ggggacattt cttggataaa
     5821 gacaaactgc atattatgac tgcttatgta tggggtcttt ccgataaaga cggtaaagct
     5881 ctggtgaaaa aagccgagcc tgcaccgact cccgcaccgg cggcagaacc cgcagcctct
     5941 gctcctgcag aagcagcaca agccgcgtcc gaagccaaac ctgccgcagc agaacctaaa
     6001 gccgaggaaa aagccgcacc tgctgccaaa gcggacggca aataggttta tgaaaccgtt
     6061 tgtgccgcct gccatggcaa tgcggttccg ggtattcccc atgtaggcat caaagccgat
     6121 tgggccgacc gcatcaaaaa aggcaaggac acgttcgttg cacaaacacg cgatcgaagg
     6181 tttcaatacg atgcccgcaa aaggcggtcg cggcgatttg agcgatgatg aagtgaaagc
     6241 tgcggttgac tatatggtca accagtcagg cggcaaattc taacttgact gagtttccga
     6301 tattatcggt tatgaaaatc cagattcagt attgaatctg gattttttgt tttaacgggc
     6361 atccggtttt aaacgcagac tgcctatttg ttgtaatgaa ttttgacttt agtcataaag
     6421 aattagtctt tattgtttat aatcgcgcat catcacgata gccctatttt caaaaggacg
     6481 attaatggat acacaaatca aaactgaagc cgacaatcag agcaaccggc gttatctgac
     6541 cgtttggcgg tggcattttt atgccggtct gctggttatg ccttttctta ccctgcttgc
     6601 cgttacgggt ttgggtatgc tgctgtttgc caatattacc ggtaaggagg gcgagcggat
     6661 tcatgttgtg ccgcaggcaa cggtacaacc tctgtctgtt caggcggaag cggcacgcag
     6721 tgccgttaat ccggaaactt cgtccgtcgt tcagtatatt gcgccgcgtg ccgatgatat
     6781 ggttgccgtg ttccgtgtca acaatgaggg caaggcaacg atggtcgcgg tcgatcctta
     6841 tacggcaaaa gtggtcagta ccatgccgcg caatcagggt tggtattacg cgatggatga
     6901 aatccacagc gatatgatgc tcggttcgac aggtgattat cttttggaaa cggctgcatc
     6961 gctgacgatt atcatgataa tcagcggttt gtacctttgg tgggtgaaac ggcgcggcat
     7021 caaggcgatg ctgctgccgc caaaaggcag ggcgcgttct tggtggcgga atctgcacgg
     7081 cgcgtttgga acttgggtgt cgttgatttt actgttgttc tgcctgtcgg gtattgcttg
     7141 ggcaggtatt tggggcggca agttcgtgca ggcttggagt cagttcccgg ccggcaaatg
     7201 gggtgtcgaa ccgaaccctg tttcagtcgt gccgacccac ggcgaggtat tgaatgacgg
     7261 caaggttaag gaagtgccgt gggttttgga gcttacgcct atgcctgttt cagggacgac
     7321 tgtgggcaaa gacggtatta accctgacga gccgatgaca ttggaaaccg tcgaccgttt
     7381 tgcgcgggaa atcggtttca aagggcgtta tcagttgaat ttgcccaaag gcgaggacgg
     7441 agtgtggact ttgtcgcagg attctatgag ttacgacatg atcagcccgt ttgccgaccg
     7501 cacggtgcat atcgaccagt acagcggcaa aatccttgcc gacatccgct ttgacgatta
     7561 caacccgttc ggcaaattta tggcggcaag cattgcgctg catatgggta ctttgggctg
     7621 gtggagcgtg ttggcgaacg ttttgttctg ccttgccgtc atttttatcg gtatcagcgg
     7681 ctgcgtgatg tggtggaaac gccgtccgac cggagcggtg ggcatcgttc cgccggcgca
     7741 gaaagtcaag ctgccggttt ggtggatgat ggcattgccg ctattggcaa tcgcactgct
     7801 cttcccgacc gcactgcttg ccattgccgt gatttggctg ttggatacct tgctgctgtc
     7861 gcggattcct gttttgagga gatggtttaa atgacccgat gccgtctgaa gcgttcagac
     7921 ggcattttgt ttgaaggcgg gcgggggaaa ggctatataa tcccgaatac ttgaccacag
     7981 cttctgtttg aaatcatgtt ttatctgtat caatccaacc gtcttgaaac gctggcggca
     8041 ctgtttgccc gcattcaaaa agtcaaaccg ctgaaatcgg ttttacagcc cgaacagatt
     8101 attgtgcaga gtcaggggat gcgccgctac ctcaatacct gcctcgcccg cgatttgggc
     8161 gtggcggcga atttgtcgtt cagcctgccc gccggtctga cgtggaagct gatgaaaaaa
     8221 ctgattcccg gtattccgga actcagcccg tttgcgcctg aagtgatgcg ctggcggctg
     8281 ttggatttgt tccgcagcga ggcatttcgg aatacggcag aatttgaaga tgtgaggaat
     8341 gtgctgcaag actatctggg cagcggcgaa tcggcggatt atcagcttgc tggccagctt
     8401 gcagacatat ttgaccaata cctcgtgtat cgtccgcagt ggatagacgc gtggcagcag
     8461 ggcaggatac tcagtttggg cgacgatgaa atctggcagt ccaaactgtg gcgttatctt
     8521 gatgacggca ggcagagcgc gccgcaccgt gttgcgttgt gggaaaagct gttggaatct
     8581 ttgagcagtg ataagctgcc cgagcgttat ttcgtgttcg gcatttccac gatggcgccg
     8641 atgtatttgc agcttttgca caagctgtcc gaacattgcg atgtgttcgt gtttgcactc
     8701 aatccgagtg ggatgtattg gggcaacgtc atcgaagcgg cgcaaatcct caaaggcggc
     8761 ggcgatcccg atttaactca ggcagggcat ccgctgctcg cctcattggg caagcagggg
     8821 cgcgactttt tcgacttttt gaacgaaatg gaaatagaag gagaaacgcc ggtatttgaa
     8881 gaaggcgggc gcgatacgct tttgcacgcc ctgcaaaccg atattcaaaa cctgaaaatg
     8941 ccgtctgaaa atgtgggaag cgtcggtacg gatgacggtt cgatacgtat cgtctccgcg
     9001 cacagccctt tgcgcgaatt gcagatactc aaagacaagc tgttgaaaat tctgcatgaa
     9061 catcccgatt ggcagccgca tgatatcgcc gtattaaccc cgaatatcga atcctatacg
     9121 ccttttatcg aggccgtgtt cggacaggcg cagcccggtg cgcaggcatt gccgtattcc
     9181 gtttcggacg tgaaaataag ccgccgacaa ccgctgtttc atgctttgtc atgcctgttc
     9241 gacttgttgg aaagccgatt tgaagtcgat aaagtgcttg tgcttttaga aaccgcaccc
     9301 gtgttgcgcc gtttcggact gactgaggac gatttgccgc ttttgcacga catggttgcc
     9361 gatttgaacg tccgctgggg tttggacgga gaaatgcgcg gcggcacgga tcagctgttt
     9421 acctggaagc aggcggtaga acgcatgata ttgggctgga tgctgcccga aggcggcaat
     9481 ccgatgtggc aggatgtcag cgcgtggtat gccgacgtga atcaaaccgc catgttcgga
     9541 cgttttgccg ccttcctcga aaccctgtcg gatattgtac ggatatggcg gcaacctgcg
     9601 acggttgatg aatgggtagg acgttgccga ggtttgcttg aaacattgtt ccaagccgaa
     9661 gccgatgacc aaaaggcagt ccaaaacctc gaaaacgaat gggtcaaatg gcaggcggaa
     9721 acagaattgg cgcaattttc cggacagttg ccgcaacaaa ccgtcatccg ccatatccga
     9781 cgtttcctcg acagcgaaag cgaggcaggc tttttacgcg gcggcatcac cttttgcagt
     9841 atggtgccga tgcggagcct gccgttcaaa gtcatctgcc tgttgggttt gaacgacgga
     9901 gattttcccc gcaataccaa agccgccgta ttcgacctga ttgccaaaca tcccgccaaa
     9961 ggcgaccgag cccgtcgcga tgacgaccgc tacctgttcc ttgaagctct catcagcgcg
    10021 cgtgaaatcc tgtatttgtc ctacatcggg cgcgatatcc gcaaagacga agagcttgcc
    10081 ccgtcttcgc tgttgggcga actcatcgac acagttgccg ccatgaccgg cacaaacagc
    10141 cgccaacttg cacaaaattg gatagaacag catccgctgc aagccttctc gcgccgatat
    10201 ttccaagaag gcggacgttc agacggcata ttcggcacgc gtaccgacta cgccactgcg
    10261 ctcggacaaa cgcccgaacc gccgcaaccc tttttcgacc aacccgtaga aaacgccgaa
    10321 cctgttgccg aaatcggaca ggacgaattt atccgtttct ggcgcaaccc cgtcaaagta
    10381 tggcttcagc agcagcttgc gtggagcgaa ccccatatcg gcgaagcctg ggagcctgcc
    10441 gaacccttcg agccgcaaca cgccgatcaa atcgccgaaa cctacatcga agcacggcgc
    10501 gaaggacggg attttgccca aaccgccgcc cgcatcgggg cggaaagcct cctgccgtcg
    10561 ggagagttgg gcagactttg gcagcagaac ttccaaactg ctgccaaaca aatcgacacg
    10621 gcggttttaa acagccccaa actgccaccg ctttcatatg ccataccgtc ggacgggcaa
    10681 atcctgaaag gcagcttggg caatctgtac cgctgcggac aagtgtttta cgcctacggc
    10741 aaacccaacg cgccgcaacg tatcgctttt ctgctggaac acctgatatt ttgcgccgtt
    10801 atgccgtctg aagccgaaac gcggcaaacc tttatcgtcc aatccggaga aaccgaaata
    10861 ttggcggaaa tcgcgcaaga cagggcattg cagctattgt cggaatggat ggcgtttttc
    10921 aatatcgggc aaaaccgccc gctgccgttt tttgccaaga cctcgcttgc cgctgccgaa
    10981 gcgtttgccc aaaaacaaga ttgggaagcc gccctgaaaa aagcccaaac cgcctattac
    11041 ggcaacaaag tcagcaaagg gcagaaagac tataccgaag tcgccctcgt gttcggcaac
    11101 gcaagccaaa acccactcga acaacccctg tttgaaaacc tcgcccgcct gcttgccgac
    11161 acgcttgccg cagcggaaaa aagggaaggg gccggagcag cctgacggga tggaaaggca
    11221 aatgccgtct gaaaacagca aaaaggtttt cagacggcat tttgtttatc gtgtttaagg
    11281 tgcgggatac aaagtgggtg cgccgggacc gacgggcagg cccaaaacaa atacccaaat
    11341 gcagaataag gcaatccatg caattaagaa gaaagcggaa tacggcaaca tcatagaaat
    11401 cagcgtaccc acgcccgcat cttttttgta tttgatcact gtcgccataa tcagcccgaa
    11461 ataactcatc atcggcgtaa taatattggt aacggaatca ccgatgcggt aagcggcttg
    11521 aatgacttcg ggcgcgtagc cggccaacat cagcataggg acgaaaatcg gcgcagttac
    11581 cgcccattgc gcggaggcgg agcctatcat cagattgata aaagcacaaa ttaaaataaa
    11641 accgataaac aacacgctgc cgcccaagcc gacttctttt aagaacgtcg cccctttaac
    11701 ggcaatatat tgcccaatat tcgtccaatt aaaaaatgcg acaaattgtg cggcaaaaaa
    11761 gatgatgacc aaataaagcc ccagagtact catcgattcg gccatcgcat taacgacttc
    11821 ctgttcgccg cgcaaacttc gggttacccg gccataaaca atgcccggca gcgcaaacaa
    11881 caagaaaata aaaacaacaa tcgattttaa aaacggcgaa ccggaaacca atcctgtttc
    11941 aggatgacgc aaaataccgt cggcagggac gatgctccaa gccaataggg cggataaggc
    12001 aacaaacacc acgccagccc aaattaatcc tttatattcc aaaggcgtga tttcattgga
    12061 atgccgaatg tctttttctt cttgtgacaa atctgattga taagggccca attgcggttc
    12121 gacgattttt tcagtaacaa aataaccaat caaagcaatc acaaacgtac tggctaccat
    12181 aaaaaaccag ttggcttcag ggcctacgac gtagtcggga tggatgattt gcgccgcctg
    12241 ttgggtgatg cctgccaaga gcggatcgat tgtgcctaag aacagattgg ccgaataacc
    12301 gcccgaaacg ccggcgaaag ccgcagccag accggcaagc ggatggcggc cgagggaatg
    12361 aaagatgatg gcggacaaag ggattaggac gacatagccc aattcagaag cggtattaga
    12421 taaaatccct gtaaaaacaa ccataaaagt agtgagtttg cgtggagatt ttgtgagcaa
    12481 taagcgcatt aatgcggaaa tcaagcccga tttttccgca atccccacgc ccaataaaga
    12541 aaccaacacc gttcccaacg gcgcgaaacc ggtgaaattt ttaacggtat gcgtcaggat
    12601 tttgatcaaa ccgtcagcat cgagcaggct gacaacgtga atcaaaccgt catcggcacg
    12661 tcctttcgca ccaacagggc gcggatcggg gacggatagt ccgaaatacg caccggcggc
    12721 agaggcaatc agcaataaca caatgaaaat aataaaaagc gtaaccgggt gcggcaacat
    12781 attgcccagc cattcgactg tgcgtaaaaa tcgtccgtcc cgttgcgtat cggtttgact
    12841 catctgcttc tccttaaaaa aaatatattg agtgcattaa atttttgaat gtaacatgaa
    12901 gtgttttgta agcaaactaa aataatcgta tatgtatata accgtaatag attaaaacag
    12961 aaaatgccca catcgttatt ctcataaagg cggtaatccg aaaacttgac gttaaagcct
    13021 tatcaggaat gactggaact caacaaagcg gtttcccgct ttcgcggaaa tggcgggatt
    13081 ttcggttttt gtttgcaaaa atgccggaga aaaatgcagt ttacattttg aatggtattg
    13141 ttattaattg taaataaata ttggaaggta aatgatatga aaaatgaaat tcaaaaaatt
    13201 atggacaaat atgatccatg gcatgaagat gattttgagt cgtatgaaga cattgccaaa
    13261 gatgtatcgt tgatgacaga taaaacgttc attgaacatt atttgttaga agtttattca
    13321 gaagaaaacg gacattttga ccaagagaat gtccatgcaa tgatagaaga aattaaaaat
    13381 gcaatttagt gaat
//
LOCUS       DAWHSK010000028        13356 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.028, whole genome shotgun sequence.
ACCESSION   DAWHSK010000028 DAWHSK010000000
VERSION     DAWHSK010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13356
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.028"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(56..565)
                     /locus_tag="WIC03_000563"
     CDS             complement(56..565)
                     /locus_tag="WIC03_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003662333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEZ3883108.1"
                     /translation="MTMILSILSLFFIIRLLFLAVSIKHEKALIAKGAKQYGKTNSTL
                     LAAVHTLYYLACFVWVWLSDTAFNGISLIGTLTVMASFVILSLIIKQLGEIWTVKIYI
                     LPNHQINRSWLFKTFRHPNYFLNIIPELIGIALLCQAWYVLLIGLPIYLLVLFKRIRQ
                     EEQAMATLF"
     gene            complement(896..1228)
                     /locus_tag="WIC03_000564"
     CDS             complement(896..1228)
                     /locus_tag="WIC03_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspG"
                     /protein_id="HEZ3883109.1"
                     /translation="MQILSFQPDIAERMLEGTEGESVNENAQFVRTDNGYWIAWHEGV
                     AALLAPDTPPGIPCFWVEGAESLEELCVMVERGEFDEVEEFDGDDDEWLETAQGCGHH
                     GDACACGH"
     gene            complement(1273..1380)
                     /locus_tag="WIC03_000565"
                     /pseudo
     CDS             complement(1273..1380)
                     /locus_tag="WIC03_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            1519..2355
                     /gene="cysT"
                     /locus_tag="WIC03_000566"
     CDS             1519..2355
                     /gene="cysT"
                     /locus_tag="WIC03_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HEZ3883110.1"
                     /translation="MLPLKTPGVLPGFKLSLGLTVLCLSLLVVLPFAMMAAKAAEIGW
                     GGFWNTIAEPNVLAAVWLSLRMSFYAMLTNVVFGTLVAWVLVRYEFPGKGLVNALVDL
                     PFALPTAVTGIALATLYAPNGWIGRFFEPLGIKIAFTPIGIWIALIVVSLPFIVRAVQ
                     PVLEELSGEYEEAAATLGASRWTTFRRVLLPEITPALLTGAGMMFARATGEYGSVIFI
                     AGNIPMVSEILPLIITGKLEQFDVQGASAVALFMLLVSFVILFALNVMQWALGRRSGA
                     KG"
     gene            2726..3586
                     /gene="cysW"
                     /locus_tag="WIC03_000567"
     CDS             2726..3586
                     /gene="cysW"
                     /locus_tag="WIC03_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HEZ3883111.1"
                     /translation="MKPYSANPNLTEPRRLRVLLIAAALGFLLLMLVVPLVAVFYEAL
                     KGGWDLYLKSLSDPEAWSAIKLTLITALIVVPVNAVLGVAMAWLLTRFDFRGKQLLTT
                     LLDLPFSVSPVVAGLMFVLLFGAHTALGGWLEAQGIQIIFAIPGIILATLFVTFPFVA
                     REIIPLMQAQGDSEEQAALILGASGWQMFWRVTLPNIKWALLYGIILTNARAMGEFGA
                     VSVVSGHIRGETNTVPLLVEIFYNEYNFTGAFALSGVLALLALATLAVQNIITKLQDK
                     KLAAAERNAV"
     gene            3583..4656
                     /locus_tag="WIC03_000568"
     CDS             3583..4656
                     /locus_tag="WIC03_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HEZ3883112.1"
                     /translation="MSITIQNLNKHFGNFHALKNINLNVPTGKLVSLLGPSGCGKTTL
                     LRIIAGLENADGGKILFDGQDVTAKHVRERKVGFVFQHYALFRHMNVFDNVAFGLTVL
                     PKSERPSKGQIRAKVEELLKLVQLSHLAKSYPHQLSGGQRQRIALARALAVEPKLLLL
                     DEPFGALDAKVRKELRTWLRDIHHNLGVTSILVTHDQEEALEVSDEIVVMNHGKIEQT
                     GSAEAIYRKPENAFVTEFLGETDAFEGRIEKGFWHYNGFAWKLDAQYKWQEQTATGYI
                     RPHEWQLAAENETPMICAEIEKIHAVGALTHILVKHGKQDVHITLAGSDAARYPIAEG
                     KELNLIPKQVYVFSQNELIEYSI"
     gene            complement(4713..6239)
                     /gene="ilvA"
                     /locus_tag="WIC03_000569"
     CDS             complement(4713..6239)
                     /gene="ilvA"
                     /locus_tag="WIC03_000569"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HEZ3883113.1"
                     /translation="MNTPLPYSNYLIRILTASVYDVAVETPLEPARSLSVRLKNNILL
                     KREDLQPVFSFKIRGAYNKMSKLPKDALACGVIAASAGNHAQGVALSAQRLGCRAVIV
                     MPETTPKIKVDAVKSRGGEVVLRGVSYNDAYDYAMELAEKEGLTYIAPFDDPDVIAGQ
                     GTVGMEIVSQHPDPIRAVFVPIGGGGLAAGVAAFIKQVRPEIKVIGVQTNDSCCMKQS
                     VEAGEIVHLKDVGLFSDGTAVKVVGNETFRLCKELLDEIITVDTDAVCGAVKDIFDDT
                     RSITEPAGALALAGLKAYIAREGAENQTLIAVTSGANMNFHRLRHVSERSELGEGNEG
                     IFAVTIPEERGSFLKFVNILGNRNITEFNYRYGDDEKAHIFVGLQAAGPQDLAVIGSR
                     LDEAGLPNVDLTDDEIAKIHIRYMVGGRTDKVENERLVSFEFPERPGALARFLNHMQG
                     GWNITLFHYRNHGADYGRILVGIDVPPHDAAAFDGFLESLGYSYHEETQNAAYKLFLA
                     "
     gene            6387..7556
                     /locus_tag="WIC03_000570"
     CDS             6387..7556
                     /locus_tag="WIC03_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEZ3883114.1"
                     /translation="MTAHKILPVLLSIILGVSHATAASPAPNKPAVHAAPTFQTPETL
                     TAAHIVIDLQSKQILSAKNINTPVEPAALTQLMTAYLVFKNMKSGNIRSEENLKIPES
                     AWASEGSRMFVRPGDTVSTDKLLKGMIVLSANDAALTLAGRLGNGSIENFVQQMNKEA
                     RRLGMKNTVFKNPTGLSREGQVSTAKDLAQLSEALMRDFPEYYPLFSIKSFKFKNIEQ
                     NNRNILLYRDNNVNGLKAGHTESGGYNLAVSYSGNGRHILVITLGSESAETRASDNSK
                     LLNWALQAFDTPKIYPKGKTVAQIQISGGSKKTVRAGFLKEAYITLPHKEAKMAEQIL
                     ETIQPIPAPVKKGQILGKIKIRQNGYTIAEKEIVALENVEKRSRWQRLWVRLTGQ"
     gene            complement(7681..8253)
                     /locus_tag="WIC03_000571"
     CDS             complement(7681..8253)
                     /locus_tag="WIC03_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEZ3883115.1"
                     /translation="MTYEIQASVREAQGTGASRRLRREGQIPGILYGEGQEPVAIAVD
                     HKTVFYALEKESFHTALIKLSLNGETKDVIVRDFQMHPFRREVQHIDFQAVKADQLVR
                     IRVPLHIVNAENSQAVKLQGGRVSLLNTSVEVVALPANIPAFLDLDCAEVVAGDILHL
                     SDIKLPEGVESVSLKRNENLAVATVTGKKR"
     gene            complement(8320..9303)
                     /locus_tag="WIC03_000572"
     CDS             complement(8320..9303)
                     /locus_tag="WIC03_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEZ3883116.1"
                     /translation="MAAYDSLMVFTGNANPELAQRVVRHLDISLGNASVSKFSDGEVA
                     VELLENVRGRDVFILQPTCAPTNDNLMEILTMADALKRASAGRITTAIPYFGYARQDR
                     RPRSVRVPISAKLVANMLYSAGIDRVLTVDLHADQIQGFFDIPVDNIYATPILLNDIK
                     QQRIENLTVVSPDIGGVVRARAVAKSLNADLAIIDKRRPKANVAEVMNIIGDIQGRTC
                     LIVDDMIDTANTLCKAAVALKERGAERVLAYASHAVFSGEAVSRIASSEIDQVVVTDT
                     IPLSEAAKNCDRIRQVTIAGLLAETVRRISNEESVSYLFNEEVMTGSMLLP"
     gene            complement(9470..9545)
                     /locus_tag="WIC03_000573"
     tRNA            complement(9470..9545)
                     /locus_tag="WIC03_000573"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9510..9512),aa:Gln,seq:ttg)
     gene            complement(9554..9629)
                     /locus_tag="WIC03_000574"
     tRNA            complement(9554..9629)
                     /locus_tag="WIC03_000574"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9594..9596),aa:Gln,seq:ttg)
     gene            complement(9642..9717)
                     /locus_tag="WIC03_000575"
     tRNA            complement(9642..9717)
                     /locus_tag="WIC03_000575"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9682..9684),aa:Gln,seq:ttg)
     gene            complement(9725..10570)
                     /gene="ispE"
                     /locus_tag="WIC03_000576"
     CDS             complement(9725..10570)
                     /gene="ispE"
                     /locus_tag="WIC03_000576"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEZ3883117.1"
                     /translation="MNIADGRQAFPAPAKLNLDLRITGKREDGYHNIESIFCLIDLQD
                     TVYLKLRDDGKIILHNPVDGMPQEVDLSYRAASLLQKYARNPAGVEIWLDKKIPTGAG
                     LGGGSSDAATVLLVLNRWWQCSRTTQQLIDLGATLGADVPFFIFGKNAFASGIGEKLI
                     GMDIPKQWYVIVKPPVHVSTAKIFTHEGLTRNSASSIMPTFQNLQPFRNDMQAVVFKE
                     YPEVWKAYSELSRYGFALMTGSGACVFTACQDRDSAYNIYRQVSDLYEAYLAEGLSKH
                     PWLSV"
     gene            complement(10580..11161)
                     /locus_tag="WIC03_000577"
     CDS             complement(10580..11161)
                     /locus_tag="WIC03_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein LolB"
                     /protein_id="HEZ3883118.1"
                     /translation="MKHTVSASVILLLTACAQLPQNNENLWQPSEHTRSFTAEGRLAV
                     KAEGKGSYANFDWTYQPPVETININTPLGSTLGQLCQDRDGALAVDGKGNVYQAESAE
                     ELSRQLVGFKLPIQYLHIWADGRRVAGAPYRILPDGILEQYGWTVGRTADSGGQVRTL
                     QLNNGNLNIRLVFTEIGMPSETETLERCAARTR"
     gene            complement(11301..13139)
                     /locus_tag="WIC03_000578"
     CDS             complement(11301..13139)
                     /locus_tag="WIC03_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883119.1"
                     /translation="MLPARFTILSVLAAALLAGQAYAAGAADAKPPKEVGKVFRKQQR
                     YSEEEIKNERARLAAVGERVNQIFTLLGGETALQKGQAGTALATYMLMLERTKSPEVA
                     ERALEMAVSLNAFEQAEMIYQKWRQIEPIPGKAQKRAGWLRNVLRERGNQHLDGLEEV
                     LAQADEGQNRRVFLLLAQAAVQQDGLAQKASKAVRRAALKYEHLPEAAVADVVFSVQG
                     REKEKAIGALQRLAKLDTEILPPTLMTLRLTARKYPEILDGFFEQTDTQNLSAVWQEM
                     EIMNLVSLHRLDDAYARLNVLLERNPNADLYIQAAILAANRKEGASVIDGYAEKAYGR
                     GTEEQRSRAALMAAMMYADRRDYTKVRQWLKKVSAPEYLFDKGVLAAAAAVELDGGRA
                     ALRQIGRVRKLPEQQGRYFTADNLSKIQMLALSKLPDKREALRGLDKIIEKPPAGSNT
                     ELQAEALVQRSVVYDRLGKRKKMISDLEKAFRLAPDNAQIMNNLGYSLLSDSKRLDEG
                     FALLQTAYQINPDDTAVNDSIGWAYYLKGDAESALPYLRYSFENDPEPEVAAHLGEVL
                     WALGERDQAVDVWTQAAHLTGDKKIWRETLKRHGIALPQPSRKPRK"
BASE COUNT         3260 a         3687 c         3148 g         3261 t
ORIGIN      
        1 aggcgaggca acgccgtact ggtttttgtt aatccgctat aattgatgaa acgggttaaa
       61 aaagtgttgc catcgcctgt tcttcttgtc ggatacgctt aaataagacc agcaaataaa
      121 tgggcaggcc aatcaataaa acataccacg cttgacataa taaggcgatg ccaatcagtt
      181 cgggtatgat gtttaaaaaa taattggggt ggcggaatgt tttaaacaac cacgaacgat
      241 taatttgatg atttggtaaa atatagattt taaccgtcca aatctccccc aactgcttaa
      301 taatcaatga caatatcaca aacgaagcca tcaccgtcag cgtaccaatc aaggatatgc
      361 cattaaaagc agtgtcagaa agccataccc aaacaaaaca cgccaaataa taaagcgtat
      421 gaactgccgc aagcagcgtg gaattggttt ttccgtattg tttcgcccct ttggcaatca
      481 aggctttttc atgtttaata gagacggcta aaaataacag tctgatgata aaaaacaggc
      541 ttaaaatgct taaaatcatt gtcattgatg tttttccatt gaaattgaaa taaatataaa
      601 tcggattaat ggtattttta attaatgatg tttcagacca tcatgctcta taaacaattc
      661 ccattaagtc cgcgccgcaa cctgctataa taagtctgca atcggcgcaa atcaatgctt
      721 tgcgtttatt gccatcccaa aataattgat gctgccttaa ttataatacc aagataagtt
      781 tttttattca ataaaataca aagggaagcg ttcagcccat tgcaaacaga tgcaatccac
      841 cgattattta aaaaacggca aagccttgcc cccttgcggc aagcctgcaa tgcctttaat
      901 gtccgcaggc gcaagcgtcg ccgtggtgcc cgcaaccctg tgccgtttcg agccattcgt
      961 cgtcatcgcc gtcaaactct tccacttcgt caaactcgcc gcgttccacc atgacgcaca
     1021 actcttcaag gctttccgcg ccttccaccc aaaaacaggg aatgcccggc ggcgtatcgg
     1081 gcgcaagcag tgccgctacg ccttcatgcc acgcaatcca atagccgttg tccgtacgga
     1141 cgaattgtgc gttttcgttg accgactcgc cttccgtacc ttccagcata cgttccgcaa
     1201 tgtccggctg aaaagataaa atctgcataa gtgttccttt atatgatggt tttccgtcaa
     1261 aacaaggcgt tatagtggat taaatttaaa ccagtacggc gttgcctcgc cttgccgtac
     1321 tatttgtact gtctgcggct tcgtcgcctt gtcctgattt aaatttaatc cactatattt
     1381 taaaacatcg cgcccgcttg agaaactgcc aaccgcttta taacaaattc gtctttgcac
     1441 caaactttcc attctttccg tttttcagac ggcatcgtta aagtagtcct tccttttcct
     1501 tattttcagc attgttttat gttaccgctc aaaacgcccg gcgtactgcc cggcttcaaa
     1561 ctcagccttg gtctgaccgt attgtgcctg tcgctgcttg tggtcttgcc gtttgcgatg
     1621 atggcggcga aggcggcgga aatcggctgg ggcggctttt ggaacacgat tgccgagccg
     1681 aacgtgttgg cggcggtgtg gctgagtttg cggatgtcgt tttatgcgat gctgaccaat
     1741 gtcgtgttcg gcacgttggt cgcttgggta ttggtgcgct atgaatttcc gggcaaaggg
     1801 ttggtaaacg ctttggtcga tttgccgttt gcgctgccga ctgcggtaac aggcattgcg
     1861 ttggcaaccc tctatgcgcc taatggctgg ataggccgtt ttttcgagcc tttgggcatc
     1921 aaaatcgcct ttacacccat cggcatttgg attgcgctga ttgtcgtcag tctacccttt
     1981 atcgtccgcg ccgtgcagcc ggtattggaa gaattgtcgg gagagtatga agaagcggcg
     2041 gcgactttgg gcgcaagccg ctggactacg ttccgccgtg tcctcttgcc tgaaatcaca
     2101 ccggcactct tgaccggcgc gggaatgatg tttgcgcggg caacggggga atacggttcg
     2161 gtgattttta tcgcgggcaa cattccgatg gtttccgaaa tcctgccgct gattattacg
     2221 ggcaagctgg aacagttcga cgtgcagggc gcgtcggcag tggcgttgtt tatgctgctg
     2281 gtttcgtttg tgattctgtt tgcgctgaac gtgatgcagt gggcgttggg caggcgttcg
     2341 ggcgcgaagg gttgaggtcg tctgaaatac ctgttaccgt cattcccgcg caggcgggaa
     2401 tccattggtg aatttcagct gccttattta ttttctgttt tcctgttgcc ctgtggtgga
     2461 ttcccgcctg cgcgggaatg acggcagcta gacgtttttt attcccttaa tcaataaaag
     2521 gttgtctgaa aacgaatccg ctccccaaaa cacagaattc gtaggtcgga tacttgtatc
     2581 cgacaaacat ttgcgattca gcaaccttgt cggatacaag tatccgacct actacttaat
     2641 gaacaaaaag gtcgtctgaa acccctcaat cccttttcag acgccccccc aaacatctta
     2701 aaaccaacca gagaacacca ccgccatgaa accctattcc gccaatccca acctgaccga
     2761 accgcgccgg ctgcgcgttt tgctgattgc cgccgcgctg ggctttctgc tgctgatgct
     2821 ggtcgtgccg ctcgtcgccg tgttttacga agccttaaaa ggcggttggg atttgtacct
     2881 gaaatcctta agcgatcccg aagcgtggtc tgccatcaaa ttgacgctga ttactgcgct
     2941 gattgtcgtt cccgtcaatg ctgtattggg tgtggcgatg gcgtggctgc tgacccgttt
     3001 tgacttccga ggcaagcagt tgctgactac cctgctcgat ttgccgtttt ccgtatcgcc
     3061 cgtggtggcc ggtttaatgt tcgtcttatt gttcggcgcg cacacggcat tgggtggctg
     3121 gctcgaagcg caaggcatac agattatctt cgccatcccc ggcattattc tggcgacgct
     3181 gttcgttacc ttcccctttg tcgcgcgcga aatcatcccg ctgatgcagg ctcagggcga
     3241 cagcgaagaa caggcggcat tgatactcgg cgcaagcggc tggcagatgt tttggcgcgt
     3301 taccctgccc aacatcaaat gggcgttact ctacggcatc atcctcacca acgcccgcgc
     3361 gatgggcgag ttcggcgcgg tcagcgtggt atcgggacac atacgcggcg aaaccaacac
     3421 cgtcccgctt ttggtcgaaa tcttctacaa cgaatacaac ttcaccggcg cattcgccct
     3481 ctccggcgta ttggcacttt tggcactggc gacgctggcg gtgcagaaca tcatcaccaa
     3541 attacaagac aaaaaactcg ccgccgccga aaggaacgca gtatgagtat caccatccaa
     3601 aacttaaaca aacacttcgg caattttcac gcgctgaaaa acatcaacct caacgtcccc
     3661 accggcaaac tcgtttccct gctcggcccg tccggctgcg gcaaaaccac gcttttacgc
     3721 attatcgccg gactggaaaa cgccgacggc ggcaagattc tgtttgatgg gcaagacgta
     3781 accgccaagc acgtgcgcga gcgcaaagtc ggcttcgtgt tccaacacta cgcccttttc
     3841 cgccatatga acgtgtttga caacgttgcc ttcggtttga ccgtattgcc caagtccgaa
     3901 cgcccgtcca aaggacaaat ccgcgccaaa gtcgaagaat tgctcaagct cgtgcagctc
     3961 tcgcatttgg caaaatccta tccgcaccaa ctctccggcg ggcaacgcca gcgcatcgcc
     4021 ctcgcccgtg cgcttgcggt cgaaccgaaa ctcctgcttt tggacgaacc cttcggcgcg
     4081 ttggatgcca aagtacgcaa agaattacgc acctggctgc gcgacattca tcacaacctc
     4141 ggtgtaacca gcattctggt tacgcacgac caagaagaag ccctcgaagt ttccgacgaa
     4201 atcgtcgtga tgaaccacgg caaaatcgaa caaaccggca gtgccgaagc catttaccgc
     4261 aaacccgaaa acgctttcgt taccgagttc ttaggcgaaa ccgacgcatt tgaaggacgc
     4321 atcgaaaaag gcttctggca ttacaacggt ttcgcgtgga aattggacgc gcaatacaaa
     4381 tggcaggagc aaaccgccac cggctatatc cgcccgcacg aatggcagct tgccgccgaa
     4441 aacgaaaccc cgatgatttg tgccgaaatc gaaaaaatcc acgccgtcgg cgcattgacg
     4501 catattttgg tgaaacacgg caaacaggac gtgcatatca cgctggctgg cagcgatgcc
     4561 gcgcgttacc caatcgccga aggcaaagaa ttaaacttaa ttccgaaaca ggtttatgtt
     4621 ttttctcaaa acgagctgat tgaatattcg atttaatccc gaaagcacaa tgccgtctga
     4681 aaggctttca gacggcattg tgctttcaag cgtcaggcaa gaaacagctt gtacgcagca
     4741 ttttgcgttt cctcgtgata gctgtatccc agactttcca agaaaccgtc aaatgcggcg
     4801 gcatcgtgag gcggcacatc gataccgacc aaaatccgcc cgtaatccgc accatggttg
     4861 cggtaatgga agagcgtaat attccaccct ccctgcatat ggttcaaaaa acgcgccaac
     4921 gcccccggac gctccggaaa ctcaaaactg accagacgct cattttctac tttgtccgtc
     4981 cgtcctccaa ccatataacg gatatggatt ttggcaatct catcatcggt caggtcgaca
     5041 ttgggcaatc ccgcctcatc caaccggctg ccgataaccg ccaaatcctg cgggcctgcc
     5101 gcttgaagtc cgacaaagat atgcgctttt tcatcgtccc cgtagcggta gttgaactcg
     5161 gtaatattcc tatttcccaa tatattgaca aacttaagga agctgccgcg ttcttcgggg
     5221 atggtaacgg caaaaatacc ttcgttgccc tcgcccaatt cactacgctc cgacacatgg
     5281 cgcaaacggt gaaaattcat attcgcaccg ctggtaacgg caatcagggt ttggttttcc
     5341 gcgccttctc gggcgatata ggctttcaga cccgccaacg ccaacgcgcc cgccggctcg
     5401 gtaatgctgc gcgtgtcatc gaaaatatcc ttgaccgcgc cgcaaaccgc atcggtatcg
     5461 actgtaatga tttcatccaa aagttctttg cagaggcgga aggtttcgtt tccgacgact
     5521 ttgaccgccg tgccgtctga aaacagcccg acatctttca aatggacgat ttcacccgct
     5581 tcgaccgact gcttcataca gcaggaatcg ttggtctgaa cgccgataac tttgatttcg
     5641 gggcggacct gcttgataaa tgccgccacg cccgccgcca aaccgccacc gcctatcggt
     5701 acgaatacgg cgcggattgg atcgggatgc tggctgacaa tttccatccc caccgtcccc
     5761 tgtcccgcaa tcacatcagg atcatcaaac ggcgcgatat aggttaaccc ttctttttcc
     5821 gccaactcca tcgcataatc gtaggcatcg ttgtatgaaa cgccccgcaa aaccacctcg
     5881 ccgccacggc ttttgaccgc atccactttg attttcggcg tagtttccgg cataacgata
     5941 acggcacggc agcccaaacg ctgtgcggac aatgccacgc cttgagcatg attgcccgcg
     6001 cttgccgcaa tcacgccgca agcgagcgca tctttcggca acttggacat tttgttgtac
     6061 gcgccgcgta ttttgaacga aaaaaccggc tgcaaatctt cgcgtttcaa aaggatgttg
     6121 tttttcaaac gtacagaaag gctgcgtgcc ggttccaaag gcgtttcgac cgccacatca
     6181 tagacagatg ccgtcaggat gcggatgagg taattggaat aaggaagggg cgtgttcata
     6241 attcaatatg ggataatcgg tttattaaaa tcgcaaaacc caaaaccata cgcccaagac
     6301 ggcgcgaaag caagaaaaat ccgcccgatc agacacccta agcgtataat cggcagactg
     6361 aaacacgcac acaattagaa tatttcatga cagcacataa aatcctgccc gtcctgcttt
     6421 ccatcatctt aggcgtttct cacgcaacgg ccgcatcgcc cgcgcccaac aaaccggcgg
     6481 tacacgccgc ccccacgttc caaacacccg aaaccctcac agcggcacac atcgttatcg
     6541 accttcaaag caaacagatt ttatccgcca aaaacatcaa tacccctgtc gaaccggcgg
     6601 cactaaccca actgatgacc gcatatctgg ttttcaaaaa catgaaatcg ggaaatatcc
     6661 gatctgaaga aaacttaaaa atacccgaat ccgcatgggc ttcagaagga agcagaatgt
     6721 tcgtacgtcc cggcgatacg gtcagcaccg acaaactctt aaaaggcatg attgtcctat
     6781 ccgcaaacga tgccgcccta acccttgccg gccgactggg caacggctcg attgaaaatt
     6841 ttgtgcaaca aatgaacaaa gaagcccgac gcttgggcat gaagaacact gtattcaaaa
     6901 atccgacagg cttgagtaga gaaggacagg tttccaccgc caaagacctc gcccagctgt
     6961 ctgaagcatt gatgcgcgac tttccggaat attacccgct gttttccatc aaatctttca
     7021 aattcaaaaa tatagaacaa aacaaccgca atatcctttt atatagggac aacaatgtaa
     7081 acggtctgaa agccggacac acagaaagcg gcggctacaa ccttgccgtg tcatactccg
     7141 gcaacggcag gcacatcctt gttatcacat taggttcgga atcggcggaa acacgcgcct
     7201 cagacaacag caagctgctg aactgggcat tgcaagcctt cgatacgccc aaaatatatc
     7261 cgaaaggcaa aaccgttgcc caaatccaaa tttccggagg cagcaaaaaa accgtccgcg
     7321 caggcttcct caaagaagcc tacatcactc tgccacataa ggaagcgaaa atggcagaac
     7381 agattttgga aaccatacag ccgattcccg ccccggtaaa aaaagggcaa attttaggaa
     7441 aaatcaaaat cagacaaaac ggatacacca ttgccgaaaa agaaatcgtc gcactggaaa
     7501 atgtagaaaa aagaagccgg tggcaaaggc tttgggtgcg tctgacaggg cagtaatccg
     7561 ccgtttcaaa tatcccgctt ttccaacaaa taaagaaatg ccgtctgaaa cacggttcag
     7621 acggcataaa acaacagggc ggtacgtatt gcatacgcgc cgccctgctg ctgaaatcaa
     7681 ttagcgtttt ttaccggtaa cagtggcaac agccagattt tcgttacgtt tcagggaaac
     7741 gctttctaca ccttcaggca gtttgatgtc tgacaagtgc agaatgtcgc cggcaaccac
     7801 ttcggcacaa tccaaatcca agaaagcagg gatgttggca ggcaaagcaa ctacttcaac
     7861 agaagtgttt aacagagata cgcggccgcc ttgcagtttg accgcttggg aattttcagc
     7921 gttaacgatg tgcaggggaa cacggatacg tacaagttga tcggctttca cagcttggaa
     7981 gtcgatgtgt tggacttcgc ggcggaacgg gtgcatttgg aaatcgcgga cgataacgtc
     8041 tttggtttcg ccgttcagag acaatttaat caacgcagta tggaaagatt ctttttccaa
     8101 tgcgtagaat acagttttgt gatccacagc gattgcaacg ggctcttggc cttcaccgta
     8161 caaaatgccg gggatttggc cttcgcgacg caggcggcgg ctcgcaccag tgccttgtgc
     8221 ttcacgaaca gaggcttgaa tttcataagt catgttaaat actccaattt aaataaaact
     8281 accgtcatcg gccgcgacca gcttaagacg gcttcgggct tatggcagca acatgctgcc
     8341 tgtcatcact tcttcattga aaagatatga gacggattct tcattgctaa tgcggcggac
     8401 ggtttcggcc aacagaccgg caatcgttac ctgacggata cggtcgcagt ttttagccgc
     8461 ttcagacaaa ggaatggtat cggttacgac cacctggtcg atttcggatg aggcgatacg
     8521 gctgaccgcc tctccggaga atacggcgtg gctggcatag gctagaacac gttcagcccc
     8581 ccgctctttc agggcgacgg cggctttgca cagcgtattt gcagtgtcaa tcatatcgtc
     8641 cacaatcaga caggttctac cttgaatatc gccgatgatg ttcatgactt ccgccacatt
     8701 ggctttcggg cggcgtttgt cgatgattgc caagtcggca ttcagggatt ttgccacggc
     8761 gcgggcgcgg acgacaccgc cgatgtccgg gctgacgacg gtcagatttt caatccgctg
     8821 ttgtttgatg tcgttcaaca gaatcggggt ggcataaata ttgtccaccg gaatatcgaa
     8881 gaaaccttga atctggtcgg catgcaaatc gacagtcaaa acacggtcga tccctgccga
     8941 atacagcata tttgccacca gtttggcaga aatcggaacg cggacggaac gcggacggcg
     9001 gtcttggcgc gcatagccga aatacggaat ggctgtggta atacgacctg ccgaagcacg
     9061 cttcagtgca tccgccatcg tcaggatttc catcaggttg tcattggtcg gcgcacaggt
     9121 cggctgaagg atgaaaacat cgcgcccgcg tacgttttcc aacagttcga cggcaacttc
     9181 gccgtctgaa aacttggata cggaagcatt gcccaaagaa atgtccaaat gcctgacaac
     9241 acgttgtgcc aattcgggat tggcattgcc tgtaaatacc atcaaactgt cgtacgcagc
     9301 catattctca cctgatttta tgtttaactt ccgctcagaa aacacaatgc ttccctctgc
     9361 ttgtaccaag cccgacatta tacctgcaac atccgacaca aacaaaggca tttcaatatt
     9421 tttatttcta tgaaataaaa gcgtgtaagc aggcttacac gcttttattt ggctggggag
     9481 gaaggattcg aaccttcgca tgctggaatc aaaatccagt gtcttaaccg cttgacgact
     9541 ccccaaaaag ggctggctgg ggaggaagga ttcgaacctt cgcatgctgg aatcaaaatc
     9601 cagtgtctta accgcttgac gactccccaa ctcgtttgac ttggctgggg aggaaggatt
     9661 cgaaccttcg catgctggaa tcaaaatcca gtgtcttaac cgcttgacga ctccccaaca
     9721 atgtttatac ggacaaccaa ggatgttttg aaagaccctc tgccaaatat gcctcgtaca
     9781 aatctgaaac ttgtcggtat atattgtatg cgctatccct atcttgacac gccgtgaata
     9841 cacacgcacc agaacctgtc attaaggcaa atccatatcg ggacaattcg gaataggctt
     9901 tccaaacttc agggtattct ttaaatacaa ctgcctgcat atcatttcta aacggttgca
     9961 ggttttggaa agtcggcatt atgcttgagg cggaatttcg tgtcaagcct tcgtgtgtga
    10021 aaatttttgc agtggaaacg tggacgggcg gtttgacgat gacataccac tgtttcggaa
    10081 tatccattcc tatcagtttt tcacctatcc cgcttgcaaa agcatttttt ccgaaaataa
    10141 aaaatggtac gtccgcaccc aaagttgcgc ccaagtcaat cagctgctgc gtcgttcggg
    10201 aacactgcca ccaacggttc aacaccagca aaacggttgc cgcatccgag cttccgccgc
    10261 ccaaacccgc ccctgtcggg atttttttgt ccagccatat ttccacgccg gcggggttgc
    10321 gcgcatattt ttgcagcaac gatgcggcac ggtagcttaa atctacttcc tgcggcattc
    10381 catcaaccgg attgtgcagg atgattttgc cgtcgtccct tagtttcaaa tatacggtat
    10441 cctgcaaatc tatcaggcag aatatgcttt cgatattgtg ataaccgtct tcccgctttc
    10501 cagtaatcct caaatcgaga ttcagttttg caggtgcggg aaacgcctgc cgtccgtccg
    10561 caatattcat ctgtccgcct tatctcgtgc gcgccgcaca gcgttccagg gtttcagttt
    10621 cagacggcat accgatttcg gtgaaaacca gcctgatgtt caaatttccg ttattcagtt
    10681 gcaacgttcg gacttgcccc ccactgtcgg cggttctgcc gacagtccaa ccgtattgtt
    10741 ccaatatgcc gtccggcagg atgcggtaag gcgcgcccgc cacacgcctg ccatctgccc
    10801 agatatgcag atattggatt ggtagtttga aaccgaccag ctgcctgctt aattcttccg
    10861 cactttccgc ctgatagaca tttcctttgc cgtccactgc caatgcgccg tccctgtctt
    10921 gacacaactg cccgagcgta ctgcccaaag gggtattgat attgatggtt tccacgggcg
    10981 gttggtatgt ccaatcgaaa tttgcatacg aacctttccc ttccgctttc actgccaacc
    11041 gcccttccgc cgtgaaactg cgggtgtgtt cggacggctg ccacaggttt tcgttatttt
    11101 gaggtaattg cgcgcaagcg gtcaaaagca ggatgaccga tgcggataca gtgtgtttca
    11161 tcagaatttc cttaacgggt gccatcctgc cggccggcgg attcggcgtg ccgtgcaaaa
    11221 cgcgtgcgaa ctgcgtgccg tttgcccgaa tgccgcagct tcccggcaaa ccttatgccg
    11281 tctgaaagga tggacctgca ttatttccgg ggtttgcggg aaggttgggg caatgcgatg
    11341 ccgtgacgtt tgagcgtttc ccgccatatt ttcttgtctc ccgtaaggtg tgccgcctgc
    11401 gtccatacgt caaccgcctg atcgcgttcg cccaatgccc acaacacttc gcccaaatgg
    11461 gcggcaactt cgggctcggg gtcgttttca aacgaatacc gcagatacgg cagcgcgctt
    11521 tccgcgtcgc ctttcaggta atacgcccag cctatgctgt cgttgacagc ggtatcgtcc
    11581 gggttgattt ggtatgccgt ctgaagcagg gcgaagcctt cgtccaaacg tttggaatcg
    11641 gaaagcaggc tgtagcccag attattcata atctgagcgt tatcgggtgc aagcctgaac
    11701 gccttttcaa gatctgaaat catttttttc cgcttgccaa gccgatcgta aacaactgac
    11761 cgctgtacca atgcctctgc ctgtaactct gtattactgc cggcaggcgg tttttcgata
    11821 atcttgtcca accccctcaa agcctcccgt ttatcgggca gcttcgacag ggcgagcatc
    11881 tgtattttgg acaaattgtc tgccgtaaaa taccgcccct gctgttcggg aagtttccgc
    11941 accctgccga tctgccgcaa agccgccctg ccgccgtcca actcgacagc cgccgcagcc
    12001 gccagcacac ctttgtcgaa caggtattcc ggcgcggaca cttttttcaa ccactgcctg
    12061 actttggtgt aatcccttcg gtcggcatac atcatcgccg ccattagcgc cgccctgctc
    12121 cgctgttcct ccgtccccct gccgtatgcc ttttcggcgt agccgtcgat aacggaagca
    12181 ccttcttttc ggtttgccgc caatatcgct gcctgaatat acaggtctgc attcggattg
    12241 cgttccaaca gcacgttcaa acgcgcatag gcatcatcca gcctgtgcag ggaaaccaga
    12301 ttcataattt ccatttcctg ccagacggcc gaaaggtttt gggtgtctgt ctgctcgaaa
    12361 aagccgtcga gtatttcggg atatttgcgt gcagtcagac gcaacgtcat taaagtgggg
    12421 ggcaatattt ccgtatcgag cttcgccaaa cgctgcaaag ctccgattgc cttttccttt
    12481 tcgcgtccct gtacgctgaa caccacatcg gcaaccgccg cttcgggcag atgttcatat
    12541 ttcaacgccg cgcggcgaac cgctttcgat gctttttgcg ccaacccgtc ctgttgcacg
    12601 gcggcttgtg ccaacaataa aaacaccctg cggttctgtc cttcgtccgc ctgagccagc
    12661 acttcttcca gtccgtccag atgctgattt cctctttccc tcagcacgtt ccgcagccac
    12721 cccgcccgtt tttgcgcctt acccggtata ggctcaatct gccgccattt ctgataaatc
    12781 atttccgcct gttcaaacgc gttcagcgac acggccattt ccaaggcgcg ttcggcgact
    12841 tcgggggatt ttgtgcgttc caacatcagc atataggttg ccagagccgt tcccgcctgc
    12901 cccttttgca aggcggtttc ccctcccagc aacgtaaata tctgattaac ccgctcgccc
    12961 actgccgcaa gccgtgcgcg ttcgtttttg atttcttcct cgctgtaacg ctgctgcttt
    13021 ctgaaaacct ttccgacttc cttcggcggc ttcgcatccg ccgcgccggc ggcatacgcc
    13081 tgcccggcaa gcagggctgc cgcgagcaca gataaaatgg tgaaacgggc gggtaacata
    13141 atcttccttt atccgacagc cgtccgaacc ttcagacggc atataatcag gcgtaacttt
    13201 atcacaagca gaatcgtttg tagatttgcg aaaccccgtt caaaaaaata aatgccgtct
    13261 gaaacgcaaa tccgcttcag acggcattcc cgacgcttgc ggtttaaggg agttttattt
    13321 ccaaattgtc aatcaggcgc gtcgtcccca gacggg
//
LOCUS       DAWHSK010000029        13222 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.029, whole genome shotgun sequence.
ACCESSION   DAWHSK010000029 DAWHSK010000000
VERSION     DAWHSK010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13222
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.029"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(76..1293)
                     /locus_tag="WIC03_000579"
     CDS             complement(76..1293)
                     /locus_tag="WIC03_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HEZ3883120.1"
                     /translation="MEIILGIVMFTVIVLALALMILFAKSKLVSEGDITIKVNGEKEL
                     TMPAGGKLLGALANEGIFIPSACGGGGSCGQCRVVVKSGGGDILPTELSHINKREARE
                     GCRLSCQVNVKTDMEIEVPEEVFGVKKWECTVISNDNKATFIKELKLAIPEGEEVPFR
                     AGGYIQIEAPPHTVAYKDFDIPEKYHEDWDKYNLWQYVSKVDEPILRAYSMASYPEEK
                     GIIMLNVRIATPPPRVPDAPPGQMSSYIWSLKPGDKVTISGPFGEFFAKDTDAEMVFI
                     GGGAGMAPMRSHIFDQLKRLNSKRKITFWYGARSKREMFYVEDFDRLAAEFPNFTWHV
                     ALSDPLPKDNWDGYTGFIHNVVYENHLKNHEAPEDCEFYMCGPPIMNQSVIKMLKDLG
                     VEDENILLDDFGG"
     gene            complement(1307..1900)
                     /gene="nqrE"
                     /locus_tag="WIC03_000580"
     CDS             complement(1307..1900)
                     /gene="nqrE"
                     /locus_tag="WIC03_000580"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HEZ3883121.1"
                     /translation="MEHYLSLFIKSVFIENMALSFFLGMCTFLAVSKKVSTAFGLGVA
                     VIFVLGLSVPANQLVYSLLKDGAIVEGVDLTFLKFITFIGVIAALVQILEMFLDKFVP
                     ALYNALGIYLPLITVNCAIFGAVSFMAQREYGFGESVVYGFGAGLGWMLAIVALAGIT
                     EKMKYSDAPKGLKGLGITFIAAGLMAMAFMSFSGIQL"
     gene            complement(1904..2530)
                     /locus_tag="WIC03_000581"
     CDS             complement(1904..2530)
                     /locus_tag="WIC03_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HEZ3883122.1"
                     /translation="MADMKRLKHLMFSPFIDNNPIALQVLGICSALAVTTKLQTAIVM
                     GISVALVTGFSSFFISLVRNYIPNSIRIIVQMAIIASLVTLVDQLLQAFAYELSKQLS
                     VFVGLIITNCIVMGRAEAFAMKEPPLESLIDGIGNGAGYGMMLLVVATVRELIGSGKL
                     LGYTVFQTVQDGGWYQTNGLFLLAPSAFFIIGFLIWGLRTWKPEQAEE"
     gene            complement(2530..3306)
                     /locus_tag="WIC03_000582"
     CDS             complement(2530..3306)
                     /locus_tag="WIC03_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HEZ3883123.1"
                     /translation="MAKKFDKDSFSGTLIVVLAVSLICSVIVAGAVVGLKPIQEKQKL
                     QDKQGYILSVAGLMDKDTDIGKTFAERIEQRVVDLATGEYVKDAPKDFSARIAGKDPA
                     QSIRIKPEDDLAGIKSRAKYTEVYLVKGEDGKIGQIILPMHGNGLWSVMYGFVAIQPD
                     GNTINGITYYEQGETPGLGGEIGNPLWQQKFVGKKLFDGQGKLALHVGKGAGSDKEHG
                     VDALSGASLTSKGVQGSFAYWFGENGYIPYLNKLKSVGAQ"
     gene            complement(3299..4531)
                     /locus_tag="WIC03_000583"
     CDS             complement(3299..4531)
                     /locus_tag="WIC03_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HEZ3883124.1"
                     /translation="MGLKHFLEKIEPHFLPGGKHEKWYALYEAAATIFYTSGAVTRKA
                     AHVRDALDSKRMMILVWLALFPAMFYGMYNVGAQAFGALTPDLLQQSIANDWHYTLAD
                     ALGINMSSEAGVSGKMLFGAIYFLPIYATVFVVGGFWEVLFATVRKHEINEGFFVTSI
                     LFALIVPPTLPLWQAALGITFGVVVAKEVFGGTGKNFMNPALAGRAFLFFAYPANLSG
                     DAVWTAVDGYSGATALAQWAAHGADGLKNAVTGQTITWMDAFIGKLPGSIGEVSTLAL
                     LIGGAFIVFARIASWRIIAGVMIGMIAMSSLFNFIGSDTNAMFAMPWYWHLVVGGFAI
                     GMLFMATDPVSASFTNVGKWWYGALIGVMCVLIRVVNPAYPEGMMLAILFANLFAPIF
                     DYFVAQANIKRRKARSNG"
     gene            complement(4534..5877)
                     /locus_tag="WIC03_000584"
     CDS             complement(4534..5877)
                     /locus_tag="WIC03_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HEZ3883125.1"
                     /translation="MIKIKKGLNLPIAGRPEQVIYDGPVITEVALLGEEYAGMRPSMK
                     VKEGDAVKKGQVLFEDKKNPGVVFTAPASGKIAAIHRGEKRVLQSVVIAVEGNDEIEF
                     ERYAPEALANLSGEEVRRNLIQSGLWTALRTRPFSKIPAVDAEPFAIFVNAMDTNPLA
                     ADPTVIIKEAAEDFKRGLLVLSRLTERKIHVCKAAGADVPSENAANIETHEFGGPHPA
                     GLSGTHIHFIEPVGANKTVWTINYQDVIAIGRLFATGRLNTGRVIALGGSQVNKPRLL
                     RTVLGAKVSQITAGELVDADNRVISGSVLNGAIAQGAHDYLGRYHNQISVIEEGRSKE
                     LFGWVAPQPDKYSITRTTLGHFLKNKLFKFNTAVNGGDRAMVPIGTYERVMPLDILPT
                     LLLRDLIVGDTDSAQALGCLELDEEDLALCSFVCPGKYEYGPLLRKVLETIEKEG"
     gene            complement(6275..7696)
                     /locus_tag="WIC03_000585"
     CDS             complement(6275..7696)
                     /locus_tag="WIC03_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEZ3883126.1"
                     /translation="MRHLPLFSLMLFPASVYAADLDGANLNLLWSLPFALILLSIALG
                     PLFFSHTWHHHYGKITAFWTLLFLIPFSLVFGASAGIHTVAHALVEEYIPFILLLLAL
                     YTISGGILVWGDLNGTPKLNTALLAVGTALASIMGTTGAAMLMIRPLLKANQNRTRRV
                     HIVIFFIFLVANIGGGLTPLGDPPLFLGFLKGVDFMWTVKHMFAPVLISTAVLLTAFY
                     FIDNRFFKQESIAQDTPAQQEKPEKIAIFGKWNFLLLSGVVGAVLMSGLWKPEHPGFE
                     ILGSRYALQNLVRDVILIALTAVSMAITPKQVRAGNEFNFEPIAEVGKLFLGIFITIF
                     PVLSILKAGEAGALGGVVSLVHDTAGHPINVMYFWMSGILSAFLDNAPTYLVFFNMAG
                     GDAQALMTGTLFHSLLAVSMGSVFMGALTYIGNAPNFMVKAIAEQRGVPMPTFFGYMM
                     WSVAFLTPVFIVHTLIFFVFKLL"
     gene            complement(7711..8001)
                     /locus_tag="WIC03_000586"
     CDS             complement(7711..8001)
                     /locus_tag="WIC03_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter"
                     /protein_id="HEZ3883127.1"
                     /translation="MQIYVKINSFFVFDRLFIKDIYLFVLNKIIYQKEHIFPSTAIRL
                     PAAFRFGKLVYPDYLLYNQSENFFQKQTGSTVRLCPGGTVPVSLPRAAQGSA"
     gene            complement(8055..8414)
                     /locus_tag="WIC03_000587"
     CDS             complement(8055..8414)
                     /locus_tag="WIC03_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEZ3883128.1"
                     /translation="MIRYLLIACGCISLLLGIIGIFLPLLPTTPFVLLSAACWAKASP
                     RFHRWLHRHRYFGPMVHNWEQNGAVPRKAKIFAISMMTASCLIMFWQFPQRWWVGAVS
                     SVFCSLVAIWMWRRPES"
     gene            complement(8411..9016)
                     /locus_tag="WIC03_000588"
     CDS             complement(8411..9016)
                     /locus_tag="WIC03_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 domain-containing protein"
                     /protein_id="HEZ3883129.1"
                     /translation="MTIMNINTSENKDAVAEHTGQWLEKAVIGLNLCPFAKAPHVKNL
                     VRIAISEAKHLDSFLEDLDEELQRLGNTPATELETTLLVHPTLFPDFDVFNDMLDIAD
                     AAVIENGLEGIVQIAPFHPQFRFQNTEPDDIGNYTNRSPYPTLHLIREDSIAKAAQAF
                     PDASAIFERNIALLEKMGHEGWAKLGITSCPYPHNKKNISK"
     gene            complement(9063..9545)
                     /locus_tag="WIC03_000589"
     CDS             complement(9063..9545)
                     /locus_tag="WIC03_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEZ3883130.1"
                     /translation="MPQLTLDKTDIKILQVLQENGRLTNVELSERVALSPSPCLRRLK
                     QLEDAGIVRQYAALLSPESVNLGLQAFIRVSIRKAKDAREDFAASVRKWPEVLSCFAL
                     TGETDYLLQAFFTDMNAFSHFVLDTLLSHHGVQDAQSSFVLKEIKHTTSLPLNHLLKE
                     "
     regulatory      9749..9846
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            10056..11156
                     /gene="gcvT"
                     /locus_tag="WIC03_000590"
     CDS             10056..11156
                     /gene="gcvT"
                     /locus_tag="WIC03_000590"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEZ3883131.1"
                     /translation="MTALKTTPFHQAHQDAGAKLVDFAGWELPIHYGSQIAEHEAVRT
                     DAGMFDVSHMLVTDVAGANAKAFFRKLIANDVAKLAFVGKALYSALLNDNGGVIDDLI
                     VYRTNEAETQYRIVSNGATREKDTAQFHKVGQEFGVAFNPRYDLGMLAVQGPKAIEKL
                     LTVKPEWADVIHGLKPFQGADLGNDWFVARTGYTGEDGVEVILPGTEAGVFFKALQAA
                     GVQPCGLGARDTLRMEAGMNLYGNDMDDNTSPLEAGMGWTVDLKDESRDFVGKAALVA
                     LKEKGVAVKQVGLLLEKGGILRAHMEVLTDKGQGETTSGVFSPSLKQSIAIARVPKDF
                     DGDTAKVLMRGKEVDVRVLKLPFVRNGQKQFD"
     gene            11318..11704
                     /gene="gcvH"
                     /locus_tag="WIC03_000591"
     CDS             11318..11704
                     /gene="gcvH"
                     /locus_tag="WIC03_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEZ3883132.1"
                     /translation="MSNNIPAELKYVASHEWLRLEEDGTITVGITHHAQELLGDIVFV
                     ELPEVGANLAAEEQAGVVESVKAASDVYAPIAGEVVAVNEDLPSAPETANSDPYGAGW
                     FFKLKPANVADYDSLLTAEQYAGEVD"
     gene            11856..13103
                     /locus_tag="WIC03_000592"
     CDS             11856..13103
                     /locus_tag="WIC03_000592"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEZ3883133.1"
                     /translation="MQLTAVGLNHQTAPLSIREKLAFAAACLPEAVRNLARSKAATEA
                     VILSTCNRTELYCVGDSEEIIRWLADYHSLPIEEISPYLYTLGMQETVRHAFRVACGL
                     DSMVLGEPQILGQIKDAVRVAQEQESMGKKLNALFQKTFSVAKEVRTDTAVGENSVSM
                     ASASVKLAEQIFPDIGDLNVLFIGAGEMIELVATYFAAKSPRLMTVANRTLARAQELC
                     DKLGVNAEPCLLSDLPAILHDYDVVVSSTASQLPIVGKGMVERALKQRQSMPLFMLDL
                     AVPRDIEAEVGDLNDAYLYTVDDMVNIVQSGKEARQKAAAAAETLVSEKVAEFVRQQQ
                     GRQSVPLIKALRDEGEKARKQVLENAMKQLAKGATAEEVLERLSVQLTNKLLHSPTQT
                     LNKAGEEDKDLVHAVAQIYHLDK"
BASE COUNT         3228 a         3302 c         3656 g         3036 t
ORIGIN      
        1 gaaaggtgtt cggacggtat cggatagaaa aaggaagaaa ccgaggtttc ttccttttgt
       61 atttgaggtt gggttttaac cgccgaaatc gtccaagagg atgttttcgt cttccacgcc
      121 caagtctttg agcattttga tgacggactg gttcataatc ggagggccgc acatataaaa
      181 ttcgcagtct tccggtgctt cgtggttttt caggtggttt tcgtaaacca cgttgtggat
      241 gaagcctgtg tagccgtccc agttgtcttt gggcaacggg tcggacaggg cgacgtgcca
      301 cgtgaagttc ggaaactctg ccgcgagtcg gtcgaagtct tcgacataga acatctcgcg
      361 tttggaacgc gcgccgtacc agaaggtgat tttgcgtttg gagttcaaac gtttcaactg
      421 gtcgaaaatg tgggaacgca tcggcgccat acctgcgccg ccgccgatga ataccatttc
      481 ggcatcggtg tctttggcga agaattcgcc gaacgggccg gaaatcgtaa ctttgtcgcc
      541 gggcttgagc gaccagatgt aggacgacat ttgtcccgga ggcgcatcgg gtacgcgcgg
      601 aggcggcgtg gcgatacgca cgttcagcat aatgatgcct ttttcctcag gatacgaagc
      661 catagagtag gcgcgcaaaa tcggttcgtc cactttggaa acgtattgcc acagattgta
      721 tttgtcccag tcttcgtggt atttctcggg aatgtcgaag tctttgtagg caacggtgtg
      781 cggcggggct tcgatttgga tgtagccgcc ggcgcggaag ggcacttctt cgccttcggg
      841 aatggcaagc ttgagttctt taatgaacgt ggccttgttg tcgttggaga tgacggtgca
      901 ttcccatttt ttcacaccga acacttcttc ggggacttca atctccatat cggttttgac
      961 gttgacttgg cacgacagcc ggcagccttc gcgcgcttcg cgtttgttga tgtgggacag
     1021 ctcggtcggc aggatgtcgc cgccgccgct ttttacgacg acgcggcact gaccgcacga
     1081 accgcccccg ccgcaggcgg aggggataaa gatgccttcg ttggcaagcg cgcccaagag
     1141 tttgccgccg gcgggcatcg tcagctcttt ttcgccgttg actttgatgg tgatgtcgcc
     1201 ttcgctgacc agcttggatt tggcaaacag aatcatcagt gccaaagcca aaacgatgac
     1261 ggtaaacatc acgataccta aaataatctc cataccggtc cctttcttat aactggatgc
     1321 cggagaacga cataaacgcc atcgccatta ggccggcggc gataaaggta atgcccaagc
     1381 ctttgaggcc tttgggagcg tccgaatatt tcattttttc ggtaatgcct gccaaagcga
     1441 caatcgccaa catccagccc aagcccgcgc cgaagccgta cacgacggat tcgccgaagc
     1501 cgtattcgcg ctgcgccata aacgatacgg caccgaaaat cgcgcagttc acggtaatca
     1561 gcggcaggta gatgcccagc gcgttataga gggcggggac gaatttgtcc aagaacattt
     1621 ccaaaatctg caccaaagcg gcaatcacgc cgatgaaggt aatgaatttc aaaaaggtca
     1681 aatccacgcc ttcgacaatc gcgccgtctt tgagcagcga gtaaacgagt tggttggcag
     1741 ggacggacag cccgagtacg aaaatgaccg ccacacccaa accgaatgcg gtggatactt
     1801 ttttggatac cgccaaaaac gtgcacatac ccaaaaagaa ggatagcgcc atattttcaa
     1861 tgaagacgga tttgatgaag aggctcaaat agtgttccat agcttattcc tccgcctgtt
     1921 cgggtttcca ggtacgcagt ccccaaatca aaaagccgat gatgaagaac gcgctggggg
     1981 cgagcaggaa caagccgttg gtctgatacc agccgccgtc ctgcacggtt tggaaaacgg
     2041 tgtagcccaa gagtttgccc gagccaatca gttcgcggac ggtggcgacg acaagcagca
     2101 tcatcccgta gcccgcgccg ttgccgatgc cgtcgatcag gctttccagc ggcggctctt
     2161 tcatcgcaaa tgcttcggcg cggcccatca cgatacagtt ggtaataatc agaccgacga
     2221 atacggaaag ctgtttggac aattcgtagg caaaggcctg taagagttgg tcgaccagcg
     2281 taaccagcga cgcgataatc gccatttgca cgataatacg gatgctgttg gggatgtagt
     2341 tgcgtaccag cgaaatgaag aagctggaaa aaccggttac caaagctacg gaaataccca
     2401 tcacgatggc cgtctgaagt ttggtggtaa ccgccaaagc cgaacaaata cccaaaacct
     2461 gcaaggcaat cgggttgttg tcgataaagg gtgaaaacat caagtgtttc aagcgtttca
     2521 tatcagccat tattgtgctc ctactgattt caatttgttc aggtagggga tatagccgtt
     2581 ttcgccaaac cagtaggcga acgaaccttg cacgcctttg gaagtcagcg acgcgccgga
     2641 gagggcatct acgccgtgtt ctttgtccga acccgcgcct ttgccgacgt gcagggcgag
     2701 tttgccttgt ccgtcaaaca gttttttgcc gacgaatttt tgctgccaca acggattgcc
     2761 gatttcgccg cccaagcccg gggtttcgcc ttgttcgtag taggtaatgc cgttgatggt
     2821 gttgccgtcc ggttgaatgg cgacaaagcc gtacatgacc gaccacaaac cgttaccgtg
     2881 catcggcagg atgatttggc cgattttgcc gtcttcgcct tttaccaaat aaacctcagt
     2941 gtatttggca cggcttttga tgcctgccaa atcgtcttcg ggtttgattc ggatgctttg
     3001 cgcggggtct ttgcctgcga tgcgcgcgct gaagtctttt ggcgcgtctt tcacatattc
     3061 gccggtcgcc aaatcgacca cgcgttgctc gatacgctcg gcaaaggttt tgccgatgtc
     3121 ggtgtcctta tccatcaaac cggccacgct caaaatatag ccttgtttgt cttggagttt
     3181 ttgtttttct tggatgggtt tcaagccgac gaccgcaccg gcaacgatga ccgagcaaat
     3241 caggctgacc gccaacacga caatcagcgt gccgctgaag ctgtctttat cgaatttctt
     3301 agccattgct gcgcgccttt ctgcgtttga tgttcgcttg tgcgacgaaa tagtcgaaaa
     3361 tcggggcaaa caggttggca aacagaatcg ccaacatcat gccttcgggg taagcggggt
     3421 tgaccacacg gatcaatacg cacatcacgc cgatcagcgc gccgtaccac catttgccga
     3481 cattggtaaa ggaggcggaa acagggtcgg tcgccataaa cagcataccg atggcgaagc
     3541 cgccgacgac cagatgccag taccaaggca tagcaaacat agcgttggtg tccgaaccga
     3601 tgaagttgaa cagcgaagac atggcaatca taccgatcat cacgccggca ataatgcgcc
     3661 aagaagcgat gcgggcaaac acgataaacg cgccgccgat taagagcgcc aaagtggaga
     3721 cttcgccgat ggagccgggc agtttgccga taaacgcgtc catccaagtg atggtttgac
     3781 cggttacggc gtttttcagg ccgtctgcac cgtgtgccgc ccattgcgcc agcgcggttg
     3841 caccggaata gccgtcaacc gccgtccaaa ccgcatcgcc gctcaagttg gctgggtagg
     3901 cgaagaacag gaaggcgcgg cctgccagcg cagggttcat aaagttttta cccgtaccgc
     3961 cgaatacttc ttttgcaacc accacgccga aggtaatacc caaggcagcc tgccacagcg
     4021 gcagcgtggg cggaacgatt aaggcaaaca gaatcgaagt cacgaagaaa ccttcgttga
     4081 tttcgtgttt gcgcacggtg gcgaacaaga cttcccaaaa accgcccacg acaaatacgg
     4141 tcgcgtaaat cggcagaaag taaatcgcgc cgaacagcat tttgcccgac acacccgctt
     4201 cagacgacat attgatgccc aaagcgtcgg caagggtgta atgccagtcg ttggcgatgc
     4261 tttgttgcag caaatcgggc gttaaggcac cgaatgcctg tgcgccaacg ttgtacatgc
     4321 cgtaaaacat ggcggggaac aaagccagcc acaccaaaat catcatgcgc ttggagtcga
     4381 gcgcgtcgcg gacgtgtgcc gctttgcgcg ttaccgcacc ggatgtgtag aaaatcgtcg
     4441 ccgccgcttc gtagagggca taccattttt catgtttgcc gcccggcagg aagtgcggtt
     4501 cgattttttc cagaaaatgt ttcaagccca taatcagcct tccttctcaa tggtttccag
     4561 cactttgcgc aacagcgggc cgtattcgta tttgcccggg cagacgaagc tgcacaaagc
     4621 gaggtcttct tcgtccaatt ccaagcaacc caatgcctgc gcgctgtcgg tatcgccgac
     4681 gattaaatcg cgcaaaagca gggtgggcag gatatccaag ggcatcacgc gctcgtaagt
     4741 accaatcggc accatggcgc ggtcgccgcc gttgacggct gtgttgaact tgaagagttt
     4801 gtttttcagg aaatggccga gggttgtacg cgtgatggag tatttgtccg gctgcggcgc
     4861 aacccagccg aacagctctt tgctgcggcc ttcttcgata acggaaatct gattgtggta
     4921 gcgtcccaaa taatcgtgcg cgccttgtgc aatcgcgccg ttcaataccg aaccggaaat
     4981 cacgcggttg tctgcgtcaa ccaattcgcc cgcagtaatt tgcgatactt tcgcacccaa
     5041 aacggtacgc aagaggcgtg gtttgttgac ttgagaacca cccaaagcaa tcacgcgccc
     5101 ggtgttcaga cggcctgttg caaacaaacg tccgatggca atcacgtctt gataattgat
     5161 ggtccaaacg gttttgttcg caccgaccgg ctcaatgaaa tgaatgtgcg tgccactcaa
     5221 accggccgga tgcgggccgc cgaattcatg tgtttcgatg ttggcagcat tttcagacgg
     5281 cacgtctgcg ccagctgcct tacaaacatg gattttgcgt tcggtcaaac ggctcaatac
     5341 caacaggccg cgtttgaaat cctcggcggc ttctttgata atgaccgtag ggtcggcagc
     5401 cagcggattg gtgtccatcg cattgacgaa gatggcgaac ggctcggcat cgacggcagg
     5461 aattttgctg aacggacggg tgcgcagcgc agtccacaaa ccggattgga tcaggttgcg
     5521 gcgcacttct tcgccgctta agtttgccag cgcttcaggt gcgtagcgtt caaactcgat
     5581 ttcgtcgttg ccttcaacgg caatcacgac tgactgaagt acgcgctttt cgccacggtg
     5641 aatcgcggcg attttgcctg aagccggcgc agtaaacacc acgcccggat tctttttgtc
     5701 ttcaaacagc acttggcctt ttttgacggc atcgccttcc ttgactttca tcgaggggcg
     5761 cataccggca tattcttcgc caagcaacgc gacttcggta atgacgggcc cgtcataaat
     5821 gacttgctcc ggtctgcccg cgatgggcag gtttagacct tttttgattt taatcatata
     5881 tttgcattac ttgtgatggt taaggtaaaa acggcgtgtt ttgataccgt gtcgcgtggc
     5941 atcaaaagca ttgaataaat taatgtagca aagtgttaga ttctatcagg aattgtacct
     6001 gtttgtcaga tttgctgctt ttttccttgc ggaagctgtt tttatagtgg attaaattta
     6061 aaccagtacg gcgttgcctc gccttagctc aaagagaacg attctctaag gtgctgaagc
     6121 accaagtgaa tcggttccgt actatttgta ctgtctgcgg cttcgtcgcc ttgtcctgat
     6181 ttttgttaat ccactataat tgtcggaagg ggggatattg atttgattat gccggaattt
     6241 aaaatgccgt ctgaatgttc agacggcata gcgtttacag cagtttgaaa acgaaaaaga
     6301 taagggtatg tacgatgaag acgggtgtca ggaaggcgac cgaccacatc atatagccga
     6361 agaaagtcgg catcggtacg ccgcgctgtt cggcaatggc cttgaccatg aagttcggtg
     6421 cgttgccgat gtaggtcagt gcgcccatga ataccgaacc catagaaacc gccagcagcg
     6481 aatgaaacag ggtacccgtc atcaaggctt gggcatcgcc gcccgccata ttgaaaaaaa
     6541 cgagataagt gggcgcgtta tccaagaatg ccgacaatat gccgctcatc caaaaataca
     6601 tcacattaat cggatgacct gccgtatcgt gaaccagcga taccaccccg cccagcgcgc
     6661 ctgcctcgcc tgctttcaga atgctcagga cgggaaagat ggtgatgaag atgccgagga
     6721 agagtttgcc cacttcggcg atgggttcaa agttgaattc gttgcctgcg cggacttgtt
     6781 tgggcgtgat tgccatagat acggcggtca atgcaatcag gatgacatcg cggacgaggt
     6841 tttgcagggc gtaacggctg ccgaggattt caaatcccgg gtgttcgggt ttccaaaggc
     6901 cggacattag aaccgcgccg accacgcccg aaagcaggag gaagttccat ttgccgaaga
     6961 tggcgatttt ttcgggtttt tcctgttgtg ccggcgtatc ttgtgcaatg ctttcctgtt
     7021 tgaagaaacg gttgtcgatg aaatagaagg cggtcaacag gacagcggtg ctgatcagga
     7081 cgggggcgaa catatgtttg accgtccaca tgaaatctac gcctttgagg aagccgagga
     7141 agagtggggg gtcgcccaaa ggggtcagac cgccgccgat gtttgcaact aggaaaatga
     7201 agaagatgac gatgtgcacg cggcgggtac ggttttggtt ggctttcagc agcggacgaa
     7261 tcatcagcat tgctgcgccg gtcgttccca tgatagaggc aagtgccgta ccgacggcaa
     7321 gcagggcggt gttgagcttg ggtgtgccgt tcaagtcgcc ccaaaccaaa atgccgcctg
     7381 aaatggtgta cagggcaagc agcagcagga tgaaggggat gtattcttca acgagtgcgt
     7441 gtgcgacggt atggataccg gcggacgcgc caaaaaccaa actgaacggg atgaggaaga
     7501 gcaatgtcca aaaggcggtg attttgccgt aatggtgatg ccaggtatgc gaaaaaaaca
     7561 agggacccaa tgcgatagac agcaaaatca gggcaaaggg caggctccac agcaggttta
     7621 ggtttgcgcc gtccaaatct gcggcgtaaa ccgatgcggg gaaaagcatt agtgaaaaca
     7681 ggggtaggtg gcgcatcgtg tttcctcgat tcaagcactg ccttgcgcgg cgcgtgggag
     7741 tgatacaggc accgtgccgc ccggacatag gcggactgtg ctgcctgttt gtttttgaaa
     7801 aaagttttcc gattgattgt aaagtaaata atcaggataa accagtttcc caaaccggaa
     7861 ggcggcggga aggcggattg ctgtgcttgg gaatatatgt tctttttgat aaataatttt
     7921 atttaaaaca aataaatata tgtctttaat aaataatcta tcgaaaacga aaaatgaatt
     7981 tattttaaca tatatttgca atgaaacagg tttgcccccc cgtttgtttg cccttatccc
     8041 tttcagtacg gcattcaaga ttcgggcctg cgccacatcc atatggcgac aagggaacaa
     8101 aaaaccgatg aaaccgcccc gacccaccag cgttggggaa actgccaaaa cattatcagg
     8161 caggatgcgg tcatcatact gatggcgaat attttggctt tgcgcggcac tgcgccgttt
     8221 tgttcccagt tatgaaccat cggaccgaaa tagcggtgcc ggtgcagcca gcggtgaaag
     8281 cgcggggatg cctttgccca gcaggcggcg gagagcagta cgaacggcgt ggtcggcaac
     8341 agcggcaaaa aaatgccgat gatacccaac agcagggaaa tgcagccgca ggcaattaaa
     8401 agataacgta tcattttgaa atatttttct tattgtgcgg ataagggcag gatgtgatac
     8461 cgagttttgc ccagccttca tgtcccattt tttccagcag ggcgatattg cgttcgaata
     8521 ttgccgaagc gtcgggaaag gcttgtgcgg ctttggcaat gctgtcttcg cggatgaggt
     8581 gcagcgtcgg atagggagaa cggttggtgt agttgccgat gtcgtcgggt tcggtattct
     8641 gaaagcggaa ttggggatgg aacggtgcga tttggacgat gccttccaag ccgttttcga
     8701 taacggcggc atcggcaatg tcgagcatat cgttgaatac gtcgaaatcg gggaataggg
     8761 tcgggtgaac cagcagggtg gtttccagtt cggtggcggg tgtattgccc agtcgctgca
     8821 gttcttcgtc caagtcttcc aaaaaactgt caaggtgttt ggcttcgctg attgcgatgc
     8881 ggacaaggtt tttaacgtgg ggggctttgg caaagggaca caggttcaga ccgatgacgg
     8941 ctttttccaa ccattgtccg gtgtgttcgg caacagcatc tttattttcg gaagtattga
     9001 tattcattat tgtcatgtaa atgtgtttgc agattgcacg tgcgggaaaa tcgggaaggg
     9061 cactattcct tcagcaggtg gttgagcggc agggaggtgg tgtgtttgat ttcttttaaa
     9121 acaaagctcg attgcgcatc ttgtacgccg tggtgggaca ggagcgtatc caaaacaaaa
     9181 tgggaaaacg cgttcatatc ggtaaaaaac gcctgaagca ggtagtcggt ttcccctgtc
     9241 agggcgaagc agctcaagac ttccggccat tttcggaccg atgcggcaaa gtcttcccgc
     9301 gcgtcttttg ctttgcggat ggaaacgcgg ataaatgcct gaagccccag gttgacggat
     9361 tccggagaca gcagcgcggc atattggcgg acgatgccgg catcttccaa ctgcttcaga
     9421 cggcgcaggc acggagaagg cgaaagtgcg acacgttcgg acagttcgac attggtcagc
     9481 ctgccgtttt cctggagaac ctgtaagatt ttaatatcgg ttttgtctaa agtgagttgg
     9541 ggcatatttg cgttccgttt taaggaattc ggattgtctg tccgtatgtt tgcggcaatc
     9601 cgcacagatg gagaccatat taacatataa aaagttatac cgtcatccgg gacaaatttt
     9661 gttttcggaa aatcatgtga aaacagaggc ggtcggtttg catctctttt aagacggctt
     9721 gcccaaaccg ccgattcaag acataatcgg gaaatgtgca ggagagtgtt acacccaact
     9781 acaatgtaac caccgaaggc gcagacaccc ttaaatcgct caggtatcag ggactgcaca
     9841 ttgaaacaaa caatctggag agcggcgttg gaataacgtc caccgaaggg gagaaggccg
     9901 tctgaaccac cattcagaca accgcgcaaa gcagtgagca gactggtttg ccatcatgcg
     9961 gatacggccg aaaatctcag gttcaaggac agatagggtc atccgcgcat tttgtgcgga
    10021 cggcatctga acaaaaaatc cggagaaact tgagaatgac cgctctgaaa accaccccgt
    10081 ttcaccaagc ccatcaggat gcaggcgcga agctagtcga ttttgccggc tgggagttgc
    10141 ccatccatta tggttcgcaa atcgccgaac acgaagccgt gcgcaccgac gccggtatgt
    10201 ttgacgtatc ccatatgctt gttaccgacg tagcaggcgc aaatgccaaa gcctttttcc
    10261 gcaaactgat tgccaacgat gtcgccaaac tcgcttttgt cggcaaagcc ctttattctg
    10321 ctttgctcaa cgacaacggc ggcgtgattg acgacttgat cgtttaccgc accaacgagg
    10381 ccgaaaccca ataccgcatc gtgtccaacg gtgcgacccg cgaaaaagac acggcgcaat
    10441 tccacaaagt cggacaagag ttcggcgttg ccttcaatcc gcgctacgac ctcggcatgc
    10501 tcgccgtaca aggcccgaaa gccatcgaaa aactcctgac cgtcaaacct gaatgggcgg
    10561 atgtgattca cggcctcaaa ccgttccaag gtgcagactt gggcaacgac tggtttgtcg
    10621 cccgcaccgg ctataccggc gaagacggcg tcgaagtgat tctgccgggt actgaagcag
    10681 gcgtattctt caaagctctg caagcagcag gcgtacagcc ttgcggcctc ggcgcacgcg
    10741 acaccctgcg catggaagcc ggtatgaacc tctacggcaa cgatatggac gacaatacca
    10801 gtccgctcga agcaggcatg ggttggaccg ttgacttgaa agacgaaagc cgcgacttcg
    10861 tcggtaaagc cgcattggtg gcattgaaag aaaaaggcgt tgccgtcaaa caggtcggcc
    10921 tgttgctcga aaaaggcggc atcctgcgcg cgcatatgga agtgttgacc gataaaggtc
    10981 aaggcgaaac caccagcggc gtattctcgc caagcctgaa acaatccatc gccatcgccc
    11041 gtgtgccgaa agattttgac ggcgataccg ccaaagtgct gatgcgcggc aaggaagtgg
    11101 acgtacgcgt actgaagctg ccgtttgtgc gcaacggaca gaaacagttt gattgatgcg
    11161 gtttcagacg gcattttcat ttcatatgcc gtctgaaagc aggttttaat tgttgtccga
    11221 tacggacgtt tgtagaaagc attgaacaag gcatctgtgg atattgattc atgcagatgc
    11281 cgtctgaaaa taacccctat caatggagta tcaaaccatg agcaacaaca tcccggccga
    11341 actgaaatac gttgccagcc atgaatggct gcgccttgaa gaagacggta cgattaccgt
    11401 cggcattacc caccacgcgc aagagctgtt gggcgacatc gtgttcgtcg agctgcccga
    11461 agtcggcgcg aaccttgccg ctgaagagca agccggtgtg gttgagtctg taaaagccgc
    11521 gtccgacgtg tacgcaccga ttgcaggcga agtcgttgcc gtcaacgaag atttgccaag
    11581 cgctccggaa actgccaaca gcgatcctta cggtgcaggc tggttcttca aactcaaacc
    11641 ggcaaacgtt gccgattacg acagtctgct gactgccgaa caatacgcgg gcgaagtgga
    11701 ttaaaccgcc cggctgcccg acggcaaccg ccggacaaac ggaaactgca ccttcagacg
    11761 gcatttttgc ggtcggaggt gcagtttttt gtccgtgttt taaggaagca gttaggctat
    11821 aataacggcc tatattcatc tttaccgatt ttttcatgca acttaccgct gtcggactca
    11881 atcatcaaac cgcaccttta agcatacggg aaaagctggc gtttgccgcg gcctgcctgc
    11941 ccgaagccgt ccgcaatctt gcccgaagca aggcggcaac ggaggcggta atcctttcta
    12001 cctgcaaccg caccgagctt tactgtgtag gcgattcgga agaaatcatc cgttggctcg
    12061 cagactatca cagccttccc atagaagaaa tcagccccta cctttatact ttggggatgc
    12121 aggagactgt gcgccatgcc ttccgcgttg cctgcggctt ggattcgatg gtgttgggcg
    12181 agccgcagat tttaggacag attaaggatg cggtcagggt tgctcaagag caggaaagta
    12241 tgggtaagaa actcaatgcc ctgttccaaa aaaccttttc tgttgctaaa gaggtccgta
    12301 ccgatactgc cgtcggcgaa aactcggttt ccatggcttc cgcttccgtc aaattggcgg
    12361 aacagatttt tcccgacatc ggcgatttga atgtcttgtt tatcggcgca ggcgaaatga
    12421 ttgagctggt tgccacttat tttgccgcca aaagtccccg gctgatgacg gttgccaacc
    12481 ggacgctggc gcgtgcacag gagttgtgcg acaagctcgg tgtcaacgcc gaaccgtgcc
    12541 tgctgtccga tctgcctgct attctgcacg attacgacgt ggtggtttct tcaacggcga
    12601 gccagcttcc gatagtcggc aaaggcatgg tcgaacgtgc attgaaacag cgtcagagta
    12661 tgccgttgtt tatgcttgat ttggccgtgc cgcgcgatat tgaagcggaa gtcggcgatt
    12721 tgaacgatgc ctatctttat acggtggacg atatggtcaa catcgtccaa agcggcaagg
    12781 aggcaaggca gaaggccgcc gccgccgccg aaacgctggt atccgagaag gttgccgaat
    12841 ttgtcaggca gcagcagggc aggcagagcg tccccttgat taaagccttg cgggacgagg
    12901 gcgagaaagc gcgcaagcag gtgttggaaa atgcgatgaa acagcttgcc aaaggcgcaa
    12961 cggcagaaga ggttttggaa cggctgtccg tccaactgac caacaagctg ctgcattcgc
    13021 cgacccaaac tttgaataag gcgggggaag aagataaaga tttggttcat gccgtcgcgc
    13081 agatttatca tttggacaaa taacggtgcg ccgggaaaaa tgccgtctga agaggtttca
    13141 gacggcattt ttttgtgccg cctgacaaca tcgtgaaatc ccacattata tcgatgtaat
    13201 cacaaagtat agtggattaa ca
//
LOCUS       DAWHSK010000030        13163 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.030, whole genome shotgun sequence.
ACCESSION   DAWHSK010000030 DAWHSK010000000
VERSION     DAWHSK010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13163
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.030"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..1707
                     /locus_tag="WIC03_000593"
     CDS             <1..1707
                     /locus_tag="WIC03_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEZ3883134.1"
                     /translation="LYTKLTRKRQEIFFNQTLAFDEIDRLFDAKAFSKFSRYTADGKQ
                     AVGEIKRHSDGTPAENLIIKGNNLIALHSLAKQFKGKVKLIYIDPPYYFVKKKQQDSF
                     GYNTNFKLSTWLTFMKNRLSIAKELLTDDGIIVISIDDDGNAYLKILLDEIFGFENFI
                     GNLPTIMNLKGNNDEYAFAGTHEYTLVFAKNKDKSTFYEFPIDEDNFLEKWEEDEIGF
                     YKKGAPMRATGTEDKREDRPEMFYPFLVKNNTVSTITDEEFSQIYNKDLEVFNDDFIQ
                     KLKEKYENLGYNFILPIADKQWGRWRWGYSIKNKARLQTDIIVSQSKNGISLYKKQRP
                     ELDDLPTKKPKTIFYKPEYSSGNGTEQMKNLFGEKAFKNPKPEELIQDFITITTNESD
                     IVLDYHLGSGTTAAVAHKMNRQYIGIEQMDYIETLAVERLKKVIDGEQGGISKAVNWQ
                     GGGEFVYAELAPFNETAKQQILACEDSDGIKTLFEGLCERYFLKYNVSVNEFSQIIQE
                     PEFQSLPLDEQKQMVLEMLDLNQMYVSLSEMDDEQFADCLNDDDKAVSRAFYQAEKKN
                     GE"
     gene            1697..4477
                     /locus_tag="WIC03_000594"
     CDS             1697..4477
                     /locus_tag="WIC03_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEZ3883135.1"
                     /translation="MANNKTLFEVIENRKAVYLEDGDDEKYRLPKFVSNNLKYPFFEW
                     QKSALENFVIFDRTSKLKDFPDIKNRPTHLLFNMATGAGKTMMMAALILYYFEKGYRH
                     FLFFVNQNNIVDKTENNFIDPTHAKFLFTEKILQGDTVIPIRKVETFSPHSDGIEIKF
                     TSIQKLYNDIHTERENQTTLADLHELNLVMLGDEAHHLNAQTKGKKQGELDLEVELKA
                     NAGNAEIERKGWEHMVLELLLNKNGNPSQNVLLEFTATLPENADVQQKYADKIITKFG
                     LKEFLQKGYTKEINLVSSTLGKKERVLHALLFAWYRHQIALKYGIANFKPVMLFRSKT
                     IDESKADYLAFLNWAENVQAVDFSFLTTFSTSLNDSDSDNANEQGKTRTEQALKFMQE
                     KGVEFAHLADWVKQNYQKHNVIITNSETNKTKTEKTDSETEKLLNNLEAADNPIRAIF
                     TVDRLTEGWDVLNLFDIVRLYEGQNGGGSNKKSGKTAAATVSEKQLIGRGVRYFPFAF
                     EGKQPNKRKFDNDMQHELRILEELFYYTHDEQSRYISELKNELRKDGYLPEKDDDKVL
                     ATFKLKSEFAANKDFRELLIWANKKIPNPNAKTNNANSLQANPQTLSFQVHGNQLLQE
                     TQFTADENDEKVKQLGTQNNFTQTIKMSEMERHIFNKALHIKGKNSQSLFHFDRLQSK
                     LNIQNRNELQNKLLKDWQIEFLGLGQDKQVCPDDKLAGCLKILEMVEKHLNESDKPFI
                     GTKEFTPKKLWEIFGTPKQKWVKKDDVKTTIATQNDWYVMNNFAGTGLEEALIQFISA
                     RLGDLKSQYDIHLIRNEEVFKLNNFANGEGFMPDFILLLKDKQKSSSNGVNDFLHYQI
                     FIEPKGEHLVETDGWKEEFLEAITVEYGMDKILQKDTPHYRLIGLPFFTDHQKNGQFT
                     ESFPLGAASLEK"
     gene            complement(4626..5798)
                     /locus_tag="WIC03_000595"
     CDS             complement(4626..5798)
                     /locus_tag="WIC03_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEZ3883136.1"
                     /translation="MKRDLSKMTCIEDLRRVAKRKMPRMFYDYIDSGSWTETTYRENT
                     SDFKDIRFRQKVLVNMEGRSLETKMIGQDVKMPVAIAPTGFTGMAHADGEILAARAAE
                     KFGIPFTLSTMSICSIEDVAENTSAPFWFQLYVMRDREFMENLIKRAKDAKCSALVLT
                     ADLQVLGQRHKDIKNGLSAPPKPTIANLINLATKPEWCMKMLNTERRTFRNIVGHAKN
                     VGDLSSLSSWTSEQFDPRLSWDDVARIKDLWGGKLIIKGIMEPEDAEKAAKSGADALI
                     VSNHGGRQLDDTVSAIKALPDIVSAVGSDIEVWMDSGIRSGQDILKAWALGAKGTMIG
                     RAFLYGLGAYGEEGVTRALEILYKEMDISMAFTGHRDIQDVDASILRGKDWGRETV"
     gene            6148..6594
                     /gene="iscR"
                     /locus_tag="WIC03_000596"
     CDS             6148..6594
                     /gene="iscR"
                     /locus_tag="WIC03_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HEZ3883137.1"
                     /translation="MRLTTKGRFAVTAMLDLAMNAQTGAVKLSAISERQNISLSYLEQ
                     LFGKLRRAGLVESLRGPGGGYILAAPAARINIAQIIAAAEDRLDATQCGSKANCHHGA
                     PCLTHDLWENLNKTINDYLGSVTLQSIIEQKNNGDGSRVVQFTHIH"
     gene            6623..7837
                     /locus_tag="WIC03_000597"
     CDS             6623..7837
                     /locus_tag="WIC03_000597"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HEZ3883138.1"
                     /translation="MTVKTPVYLDYAATTPVDKRVAEKMIPYLTETFGNPASNSHAFG
                     WEAEEAVEKARADIAALINADPKEIVFTSGATESDNLAIKGAANFYKTKGKHLITVKT
                     EHKAVLDTMRELERQGFEVTYLGVQENGLIDLEELKAAIRDDTILISVMWVNNEIGVV
                     QDIPAIGEICRERKIVFHVDAAQACGKVPVDVEAAKIDLLSMSAHKVYGPKGIGALYV
                     RRKPRVRLEAQMHGGGHERGFRSGTLPTHQIVGMGEAFRIAKEELEQDMAHYRKLRDI
                     FLKGIEGIEEVYINGDLGHRAPNNLNVSFNFVEGESLIMAVKELAVSSGSACTSASLE
                     PSYVLRALGRNDELAHSSLRITFGRMTTEEEVQFAAELIKSKIGKLRELSPLWEMFKD
                     GIDLNSIEWAAH"
     gene            7983..8828
                     /locus_tag="WIC03_000598"
     CDS             7983..8828
                     /locus_tag="WIC03_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEZ3883139.1"
                     /translation="MKIANALIHNTVRIECLSADGQSISTGTAFLFLFDFDKTGVIPV
                     LVTNKHVVFVESAKKIAITLTKDENGSPNHKENITFTIEDFIQNCLPHPDENIDLCIA
                     PVGHFFNQLINHNFSPFIKGIRESDIMQTEEMDQLSAFEEVFMIGYPNGLWDSKNNLP
                     IFRTGITATHPAIDFEGKKEFMIDVACFPGSSGSPVFKYIPAGLRKIENDGFAITADE
                     TFKLMGILYAGPQQTISGEIYITTVNKPISVSSIPINLGFVIKAEKLLDFKPILKNFL
                     EKNRE"
     gene            9171..9557
                     /gene="iscU"
                     /locus_tag="WIC03_000599"
     CDS             9171..9557
                     /gene="iscU"
                     /locus_tag="WIC03_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HEZ3883140.1"
                     /translation="MAYSDKVIDHYENPRNVGTFDKNDESVGTGMVGAPACGDVMRLQ
                     IKVNDEGIIEDAKFKTYGCGSAIASSSLITEWVKGKSLDDALAIKNSEIAEELELPPV
                     KIHCSILAEDAVKAAVADYRKRQENR"
     gene            9644..9964
                     /gene="iscA"
                     /locus_tag="WIC03_000600"
     CDS             9644..9964
                     /gene="iscA"
                     /locus_tag="WIC03_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HEZ3883141.1"
                     /translation="MITLTENAAKHINDYLAKRGKGLGVRLGVKTSGCSGMAYNLEFV
                     DEADGDDLIFEGHGARIYIDPKSLVYLDGTQVDYTKEGLQEGFKFENPNVKDSCGCGE
                     SFHV"
     gene            complement(9924..10145)
                     /locus_tag="WIC03_000601"
     CDS             complement(9924..10145)
                     /locus_tag="WIC03_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor A"
                     /protein_id="HEZ3883142.1"
                     /translation="MGGKVQHNKGKIRDNALKALVKSDLFRHKVERKRKGKGSYNRQE
                     AKKWRDGFDTVPPFLCLKRGSFRRSRRSL"
     gene            10227..10727
                     /gene="hscB"
                     /locus_tag="WIC03_000602"
     CDS             10227..10727
                     /gene="hscB"
                     /locus_tag="WIC03_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HEZ3883143.1"
                     /translation="MSQYFTLFRIEPAFDIDTENLEQTYRALAARFHPDKFASASAFE
                     QKQAVMMSSTINDAYRTLKNPIDRAAYLLKTSGIDADAPEHTAFAPEFLMQQMEWRET
                     LMEARAGNDLESLKNLDNEIRDEQEKLFCGLKQSFARQDYDTAAQQVRQGRFLDKLRN
                     EISSAL"
     gene            10825..>13163
                     /gene="gyrA"
                     /locus_tag="WIC03_000603"
     CDS             10825..>13163
                     /gene="gyrA"
                     /locus_tag="WIC03_000603"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEZ3883144.1"
                     /translation="MTDATIRHDHKFALETLPVSLEDEMRKSYLDYAMSVIVGRALPD
                     VRDGLKPVHRRVLYAMHELKNNWNAAYKKSARIVGDVIGKYHPHGDTAVYDTIVRMAQ
                     NFAMRYVLIDGQGNFGSVDGLAAAAMRYTEIRMAKISHEMLADIEEETVNFGPNYDGS
                     EHEPLVLPTRFPTLLVNGSSGIAVGMATNIPPHNLSDTVNACLRLLDAPDTEIDELID
                     IIQAPDFPTGATIYGLSGVREGYKTGRGRVVMRGKTHIEPIGRNGEREAIVIDEIPYQ
                     VNKAKLVEKIGDLVREKTLEGISELRDESDKSGMRVVIELKRNENAEVVLNQLYKLTP
                     LQDSFGINMVVLVDGQPRLLNLKQILSEFLRHRREVVTRRTLFRLKKARHEGHIAEGK
                     AVALSNIDEIIKLIKESPNAAEAKDKLLARPWRSSLVEEMLTRSGLDLEMMRPEGLAA
                     NIGLKEQGYYLSEIQADAILRMSLRNLTGLDQEEIVESYKNLMGKIIDFVDILSKPER
                     ITQIIRDELEEIKTNYGDERRSEINPFGGDIADEDLIPQREMVVTLTHGGYIKTQPTT
                     DYQAQRRGGRGKQAAATKDEDFIETLFVANTHDYLMCFTNLGKCHWIKVYKLPEGGRN
                     SRGRPINNVIQLEEGEKVSAILAVREFPEDQYVFFATAQGMVKKVQLSAFKNVRAQGI
                     KAIALKEGDYLVGAAQTGGADDIMLFSNLGKAIRFNEYWEKSGNDEAEDADIETEISD
                     GIEDETADSENALPSGKHGVRPSGRGSGGLRGMRLPADG"
BASE COUNT         4058 a         3180 c         2823 g         3102 t
ORIGIN      
        1 ttatacacta aattaacccg aaaaagacaa gaaatctttt ttaatcaaac gcttgctttt
       61 gatgaaatcg atcggctttt tgacgcaaaa gcattctcaa aattctctcg ctataccgca
      121 gacggcaaac aagcggttgg cgaaatcaaa cgtcattcag acggcacacc cgccgaaaat
      181 ctcattatca aaggcaataa tctgatagcc ctacattcgc ttgccaaaca gtttaaaggc
      241 aaagtgaagt tgatttatat tgatccgcct tattattttg ttaaaaagaa acaacaagat
      301 agttttggtt ataacactaa ctttaaatta tctacttggc ttacatttat gaaaaatcgt
      361 ttatcaattg ccaaagaatt attaactgat gatgggatta ttgttatttc cattgatgat
      421 gacggaaatg cttatttaaa gattttgtta gatgaaattt ttggatttga aaattttatc
      481 ggaaatttac ccactattat gaatttaaaa ggtaataatg atgaatatgc ttttgcaggc
      541 actcacgaat acacactagt ctttgctaaa aataaagata aatctacttt ttatgaattt
      601 ccaatagatg aagataattt tttagagaaa tgggaagaag atgaaattgg gttttataaa
      661 aaaggagctc caatgcgagc aacaggaacc gaagataaaa gagaagatag acctgaaatg
      721 ttctatcctt ttttggtaaa aaataacaca gttagtacaa ttacagatga agaattttct
      781 caaatctata ataaagattt agaagtattc aatgatgatt ttattcaaaa actaaaagag
      841 aaatacgaaa atcttggata caattttatt ttacctattg ctgataaaca atgggggcgt
      901 tggcgttggg ggtattcaat aaagaataag gcaaggctgc agacggatat tattgttagc
      961 caatctaaaa atggaatttc cttgtataaa aaacaaagac ctgaattaga tgatttacct
     1021 acaaaaaaac ctaaaactat tttttataaa cctgaataca gtagtggtaa tggaactgaa
     1081 cagatgaaaa atctttttgg tgaaaaggca tttaaaaatc ctaaaccaga agaacttatc
     1141 caagatttta ttaccatcac aaccaacgaa tccgacatcg tcttagacta ccatcttggt
     1201 agtggcacaa ccgccgccgt tgcccacaaa atgaaccgac aatatatcgg tattgaacaa
     1261 atggattata ttgaaacgct tgccgttgaa cgcttgaaaa aagtgattga tggcgagcaa
     1321 ggcggtattt ccaaagccgt gaattggcaa ggtggtggcg agtttgttta tgccgaactt
     1381 gccccattta acgaaaccgc aaaacaacaa attttggctt gcgaagattc agacggcatc
     1441 aaaacgctgt ttgaaggttt atgcgaacgc tatttcttga aatacaacgt cagcgtaaat
     1501 gaatttagtc aaatcattca agagcctgaa tttcaatctt tgccattaga cgaacaaaaa
     1561 caaatggtgc ttgaaatgtt agatttaaat caaatgtatg tttcattatc cgaaatggac
     1621 gacgagcaat ttgcagattg cctgaacgat gacgataaag cggtaagccg tgcgttctat
     1681 caagcggaga aaaaaaatgg cgaataataa aactttgttt gaagtaattg aaaaccgcaa
     1741 agctgtgtat ttggaagacg gagatgatga aaaatacagg ctgcctaaat ttgtttcaaa
     1801 taatttgaaa tatccgtttt ttgaatggca aaaatcggct ttagaaaact ttgtgatttt
     1861 tgaccgcact tcaaaattaa aggattttcc cgatatcaaa aaccgcccta ctcatttgct
     1921 atttaatatg gcaacaggtg caggcaaaac gatgatgatg gcggcattga ttttgtatta
     1981 ttttgaaaaa gggtatcggc attttctgtt ttttgtgaat caaaacaata tcgtggataa
     2041 aacggaaaat aattttattg acccgacaca cgcaaaattt ttatttaccg agaagatttt
     2101 gcagggcgat acggtaattc ccattcgcaa agtggaaacg ttcagcccac attcagacgg
     2161 cattgaaatt aaatttacca gcattcaaaa gctgtataac gatattcaca ccgaacggga
     2221 aaaccaaacc acattggcgg atttgcacga attgaatctt gtgatgctgg gcgatgaagc
     2281 acaccattta aacgcgcaaa ccaaaggcaa aaaacaaggc gagttggatt tggaagtgga
     2341 attaaaagcc aatgcaggca atgccgaaat tgaacgcaaa ggctgggagc atatggtttt
     2401 ggaattgtta ctcaataaaa atggcaatcc cagccaaaat gtgctgttgg aatttaccgc
     2461 cacgctgcct gaaaatgctg acgtgcaaca aaaatacgcg gataaaatca tcacaaaatt
     2521 tggcttaaaa gaatttttgc aaaaaggtta taccaaagaa atcaatttgg tatccagtac
     2581 gctgggcaag aaagagcggg tgttgcacgc tttattattt gcttggtatc gccatcaaat
     2641 cgctttgaaa tatggcattg ccaatttcaa gcctgtgatg ttgtttagaa gtaagacgat
     2701 tgatgaatca aaagcggatt atctggcatt tttaaattgg gcagaaaatg tgcaggcggt
     2761 tgatttttcg tttttaacta cattttcaac aagcttgaac gatagcgata gcgataacgc
     2821 caacgaacaa ggcaaaaccc gcactgaaca agccctaaaa tttatgcagg aaaaaggcgt
     2881 tgagtttgca catttggcag attgggtaaa acaaaattat caaaaacaca atgtgattat
     2941 taccaactcc gaaaccaaca aaaccaaaac cgaaaaaacc gacagcgaaa cagaaaaatt
     3001 gctgaataat ttggaagcgg ctgataatcc gattcgtgcc atttttacgg tggacagatt
     3061 aaccgaaggt tgggacgttc tgaatttgtt tgatattgtg cgtttgtatg aagggcaaaa
     3121 cggcggcggt tcaaataaaa aatcaggcaa aacggctgcc gccaccgtat cggaaaagca
     3181 gttgattggt cgtggcgtgc gttattttcc atttgcgttt gaaggtaaac agccgaataa
     3241 acgcaaattt gacaacgata tgcaacacga attgcgtatc ttggaagaat tgttttatta
     3301 cacgcacgat gagcaatccc gctatatttc agaactgaaa aacgagttgc gaaaagacgg
     3361 ttatttgcct gaaaaagacg atgataaggt attggcaaca tttaaactca aatctgaatt
     3421 tgccgctaat aaggatttta gagaattgtt aatttgggcg aataaaaaaa tccccaaccc
     3481 aaacgccaaa accaataatg caaacagcct gcaagccaat ccgcaaacgc tttcattcca
     3541 agttcacggc aatcaactgt tgcaggaaac gcaatttaca gccgatgaaa atgatgaaaa
     3601 agtcaagcaa cttggcacgc aaaataattt tactcaaacc ataaaaatga gtgaaatgga
     3661 gcggcacatt ttcaataaag ccctgcatat caaagggaaa aatagtcaat ctttattcca
     3721 ttttgaccgc ctgcaaagca aactcaatat tcaaaatcgc aatgaattgc aaaataaatt
     3781 gttgaaagac tggcaaattg aatttttagg attagggcaa gacaaacagg tttgcccgga
     3841 cgataaactt gcaggctgcc tgaaaatctt ggaaatggtt gaaaagcatt tgaatgaaag
     3901 tgataagcca tttatcggta caaaagaatt tacgcctaaa aaattatggg aaattttcgg
     3961 cacgccaaaa caaaaatggg tcaaaaaaga tgatgtaaaa acgactattg ccacgcaaaa
     4021 tgattggtat gtgatgaata attttgccgg aacgggtttg gaagaagcgt taattcaatt
     4081 tatttcagcg cgtttgggcg atttgaaatc tcaatatgac attcatttaa tccgtaacga
     4141 agaagtgttt aaattgaata actttgccaa tggcgaagga tttatgcccg actttatttt
     4201 attgctgaaa gataaacaaa aatcttcttc caatggcgtg aatgactttt tgcattacca
     4261 aattttcatt gagcccaaag gtgagcattt agtggaaact gatggatgga aagaagaatt
     4321 tttagaagcc atcacagtgg aatatgggat ggataagatc ctgcaaaaag atacaccgca
     4381 ttatcgtttg atcggtttac cgttttttac tgatcatcag aaaaatggac aatttacgga
     4441 gtcattccct ttaggtgcgg catcgcttga aaaatagagt agtgcattgc aggcaacccc
     4501 gtttgacaaa acttccttta caaaagggcg ttttgtcaga tatttaatca acacattatt
     4561 aaaatacagc caaattttaa tgccgtccga accctgtgtt cagacggcat cgtatttttc
     4621 agtatctaaa ccgtttccct accccaatct ttacccctta aaatcgaggc atcgacatct
     4681 tgaatatcgc ggtgtcccgt aaacgccata gatatatcca tttctttata tagaatttcc
     4741 agcgcacggg ttacgccttc ttcgccatac gcgcccaagc catacaggaa cgcccgacct
     4801 atcattgtac ctttcgcgcc caaagcccac gccttcaaaa tatcctgacc gctgcggatg
     4861 ccgctgtcca tccaaacttc gatgtcgctg cccactgcgc tgacgatgtc gggcaaggct
     4921 ttgatggcag acacggtatc gtcgagctgt cggccgccgt ggttggaaac aatcaatgcg
     4981 tccgcgccgc ttttcgctgc tttttccgcg tcttcgggtt ccataatgcc tttgataatc
     5041 agcttgccgc cccacaaatc tttaatgcgc gccacatcgt cccagctcag gcgcgggtcg
     5101 aattgttcgg aagtccatga agacagcgaa gacaaatcgc cgacgttctt cgcgtgtccg
     5161 acgatattgc ggaacgtgcg gcgttccgtg ttcagcattt tcatgcacca ttccggtttg
     5221 gtcgccagat tgattaaatt ggcgatggtc ggtttcggcg gcgcggacag gccgtttttg
     5281 atgtctttgt ggcgttgacc caaaacctgc aaatcggcgg tcaataccaa tgccgaacat
     5341 ttggcatcct tcgcgcgctt aatcaggttt tccataaact cgcggtcgcg catcacataa
     5401 agctgaaacc aaaacggcgc gctggtgttc tcggcaacgt cttcaatcga gcagatggac
     5461 atggtggaca acgtaaacgg aataccgaac ttctccgccg cccgcgccgc caaaatttcg
     5521 ccgtctgcgt gtgccatgcc ggtaaaaccc gtcggcgcaa tcgccaccgg cattttcaca
     5581 tcctgcccga tcattttagt ctcaaggctg cgtccctcca tattgaccaa tactttttga
     5641 cggaagcgga tgtctttgaa atccgaagta ttttcccgat aagtcgtttc cgtccacgaa
     5701 cccgaatcga tgtaatcgta aaacatacgc ggcattttgc gcttggcaac gcggcgcaag
     5761 tcttcgatgc aggtcatttt gctcaaatca cgtttcatct tgtttttccc tgtttgacag
     5821 cctgatggaa tatgccgtcc gaaaccgctt gagccttcaa acggcatttc aggcaatacc
     5881 caaataaatc actaagattt agataatcta tatcagtatc aaaaataaat caaatcgttt
     5941 tgaaaccgct tgccggcaaa ataccccgac cgccgctcct caaaaaacat aaccaactga
     6001 tttaaaaaac caaacaaata aagcaaaatc agactatacc gccatttaaa caaacaatcg
     6061 gcaacagctc ccccttactt gactgaaaca ctcagatatt ggacaattct ccccactata
     6121 aaaaagccga cacgggcaac caccaccatg agactgacca ccaaagggcg tttcgccgtt
     6181 accgctatgc tggatttggc gatgaacgcg caaaccggcg ccgtcaaact cagtgccatc
     6241 agcgaacgcc aaaacatatc cctctcctat ctcgagcaat tgttcggcaa actccgccgc
     6301 gccggactgg ttgaaagcct gcgcgggccc ggcggcggct acatcctcgc cgcaccggcg
     6361 gcacgcatca acatcgccca aatcatcgcc gccgccgaag accggctgga cgcgacccaa
     6421 tgcggcagca aagccaactg ccaccacggc gcgccctgcc tgacgcacga cctttgggag
     6481 aatttaaaca aaaccatcaa cgactacctc ggcagcgtta ccctgcaaag catcatcgaa
     6541 cagaaaaaca acggcgacgg cagccgcgtc gtccaattta cacacatcca ttaaataaca
     6601 cccgaaaaag aaagagcaaa ccatgaccgt caaaaccccc gtttacctcg actacgccgc
     6661 caccaccccc gttgacaaac gcgttgccga aaaaatgatt ccctatctga ccgaaacctt
     6721 cggcaacccc gcctccaaca gccacgcatt cggctgggaa gcagaagagg ccgtcgaaaa
     6781 agcccgcgcc gacatcgctg ccctgattaa tgccgacccc aaagaaatcg tcttcaccag
     6841 cggcgcgacc gagtccgaca acctcgccat caaaggcgcg gcaaacttct acaaaaccaa
     6901 aggcaaacac ctcattaccg tcaaaaccga acataaagcc gttttagata caatgcgcga
     6961 actcgaacgc caaggttttg aagtaaccta cctcggcgtt caagaaaacg gtttgattga
     7021 tttggaagaa ctcaaagccg ccatccgcga cgataccatc ctgatttccg taatgtgggt
     7081 aaacaacgaa atcggcgtgg tgcaagacat tcccgccatc ggcgaaatct gccgcgaacg
     7141 taaaatcgtc ttccacgtcg atgccgccca agcctgcggc aaagtgcctg tcgatgtcga
     7201 agccgccaaa atcgacctgc tctcgatgtc cgcgcacaaa gtgtacggcc ccaaaggcat
     7261 cggcgcgctg tacgtccgcc gcaaaccccg cgtccgcctc gaagcccaaa tgcacggcgg
     7321 cggccacgaa cgcggcttcc gcagcggcac attaccgacc caccaaatcg tcggtatggg
     7381 cgaagccttc cgcatcgcca aagaagaact cgaacaagat atggcgcact accgcaaact
     7441 gcgcgatatc ttcctcaaag gcatcgaagg catcgaagaa gtctacatca acggcgacct
     7501 cggacaccgc gccccgaaca acctgaacgt cagcttcaac ttcgtcgaag gcgaaagcct
     7561 gattatggcg gtgaaagaac tcgccgtatc cagcggctcc gcctgcacct ccgcctcgct
     7621 cgaacccagc tacgtcctgc gcgcgctcgg ccgcaacgac gaactggcgc actcatccct
     7681 gcgtatcacc ttcggtcgca tgaccaccga agaagaagtg caattcgccg ccgaactgat
     7741 taaatccaaa atcggcaaac tgcgcgaact gtcgccgctg tgggaaatgt tcaaagacgg
     7801 gattgatttg aactcgattg agtgggcggc gcattaattt atgaattagt acgatgatat
     7861 gagtatagta gataagattt tcaaatattc tcgccacctc gattattgag atatagtgaa
     7921 cttcttatct acggataaaa tttttataga tagtttaata tttaaataga acttaaaaaa
     7981 ttatgaaaat agccaatgca ttaattcaca atacagtccg tattgaatgc ctttcagcag
     8041 acggccaatc tatatcaacg ggaacggcat tcttattcct tttcgatttc gacaaaacag
     8101 gggtaattcc agttcttgta acaaataaac atgttgtttt tgtagagtcc gcaaaaaaaa
     8161 tagctattac tttgacaaaa gatgaaaatg gctctccaaa tcataaagaa aatataactt
     8221 ttacaattga agattttata caaaattgtt tacctcatcc tgatgaaaat attgatctat
     8281 gcattgctcc tgttggacat ttttttaatc agctgattaa tcataatttc tcgcctttta
     8341 taaaaggaat aagagaatct gatattatgc aaactgaaga aatggatcaa ttatcagctt
     8401 tcgaagaagt ttttatgata ggctatccta atggcttatg ggactcaaaa aacaacttac
     8461 caatatttag aacaggaatt actgctaccc atccagctat tgattttgaa gggaaaaaag
     8521 aatttatgat tgatgttgcc tgtttccctg gttcaagcgg ttctccggtg ttcaaatata
     8581 tacctgctgg gttaagaaaa atagaaaacg atgggtttgc aattacagca gatgaaacat
     8641 ttaaattaat gggaatccta tatgctggac ctcagcaaac aatatcaggg gaaatttata
     8701 taacaactgt taataaacca atttctgtat cttctattcc tattaattta ggttttgtca
     8761 taaaggcaga aaaactttta gactttaaac caattttaaa gaattttttg gaaaaaaata
     8821 gagaatgaaa ttatattaaa agattaataa atattagaga ctggcaatcc accttaccta
     8881 atctacatta aaaataaaaa agaccgtttg aaacatcaaa aagatttttt caaacgacct
     8941 aaaagccacc acatgaaatc aaggatacaa aaccatgaac gaacaagaac ttgacctcga
     9001 caatctcgac aacctgcttg acgattttga cggcgttacc gtcgaaggcg gcgtcgattc
     9061 ggaaaacgac gaaggctgcg aaggcggcgc gtgcaaaatc tgactcaggc cgtctgaaaa
     9121 ccggcaaaca aaccacttta aactatcaaa aacattaagg aaaccacatc atggcataca
     9181 gcgataaagt aatcgaccac tacgaaaatc cccgcaacgt cggtactttc gacaaaaacg
     9241 acgaatctgt cggcaccggc atggtcggcg cgcccgcctg cggcgacgtc atgcgcctgc
     9301 aaatcaaagt gaacgacgag ggcatcatcg aagatgcgaa atttaaaact tacggctgcg
     9361 gctcggccat cgcttcgtcc agcctgatta ccgagtgggt taaaggcaaa agcctggatg
     9421 acgcgctggc aatcaaaaac agcgaaatcg ccgaggagtt ggaattgccg ccggtaaaaa
     9481 tccactgctc catcttggct gaagatgcgg taaaagcggc cgttgccgac taccgcaaac
     9541 gtcaggaaaa cagataaagc ccttcagacg gcatcatccc gcaatgccgt ccgaaccacc
     9601 cgccgcttca ggtccgtccg gggcggtaca acaaggaaga aatatgatta cccttaccga
     9661 gaatgccgca aaacacatca atgactatct cgccaaacgc ggcaaaggct tgggcgtacg
     9721 cttgggtgtg aaaaccagcg gctgctcggg gatggcgtac aaccttgaat ttgtcgacga
     9781 agccgatggc gacgacctga ttttcgaagg acacggcgcg cgcatttata tcgatccgaa
     9841 aagcctggtt tatctggatg gcacgcaagt cgattacacc aaagaaggtt tgcaggaagg
     9901 tttcaaattt gaaaacccca atgtcaaaga ctcctgcggc tgcggcgaaa gcttccacgt
     9961 ttaaggcata aaaacggcgg gaccgtatca aaaccgtccc gccatttttt cgcttcctgc
    10021 ctgttgtagc tgcctttgcc tttccttttc cgttccacct tgtgccggaa caaatcggat
    10081 ttcactaagg cttttaaagc attgtcgcgt attttgcctt tattgtgctg cactttgccg
    10141 cccatattca gtcctttcgt ttaagaagcg gcagattata aggcaaaaac agttttctgc
    10201 caaaatctta catttatcat cctactatgt cccaatattt caccctcttc cggattgaac
    10261 ccgctttcga tatcgacacc gaaaacttgg aacaaaccta ccgcgccttg gccgcccgtt
    10321 tccatcccga taaattcgct tcagcttccg cctttgagca aaagcaggca gtgatgatgt
    10381 cttccaccat caacgatgcc taccgcacct tgaaaaaccc catcgaccgc gccgcctacc
    10441 tgctgaaaac atcgggcatc gatgccgacg cgccggagca taccgctttc gcccccgaat
    10501 tccttatgca gcaaatggaa tggcgcgaaa cgctgatgga ggcacgggca ggcaacgacc
    10561 ttgaatcctt gaaaaatctc gacaacgaaa tccgcgacga acaagaaaaa ctgttctgcg
    10621 gtctgaaaca gtcgtttgcc cgacaagatt acgacacagc cgcacaacaa gtccgccaag
    10681 gcaggtttct cgacaaactc cgcaacgaaa tttcctcggc attataatcc gcaccgtgtt
    10741 tcagacggcg taaccgccgc accgttccgc gtcaaaatat gctaaaataa gcaacaattt
    10801 tttgccatac gaaacattga aaccatgacc gacgcaacca tccgccacga ccacaaattc
    10861 gccctcgaaa ccctgccggt aagccttgaa gacgaaatgc gcaaaagcta tctcgactac
    10921 gccatgagcg tcattgtcgg gcgcgcgctg ccggacgttc gcgacggtct caagccggta
    10981 caccgccgcg tactgtacgc gatgcacgag ctgaaaaaca actggaatgc cgcctacaaa
    11041 aaatcggcgc gcattgtcgg cgacgtcatc ggtaaatacc acccccacgg cgataccgcc
    11101 gtatacgaca ccatcgtccg tatggcgcaa aatttcgcta tgcgttatgt gctgatagac
    11161 ggacagggca acttcggatc ggtggacggg cttgccgccg cagccatgcg ctacaccgaa
    11221 atccgcatgg cgaaaatttc ccacgaaatg ctggcagaca ttgaggaaga aaccgtcaat
    11281 ttcggcccga actacgacgg tagcgaacac gagccgcttg tactgccgac ccgtttcccc
    11341 acactgctcg tcaacggctc gtccggcatc gccgtcggca tggcgaccaa tatcccgccg
    11401 cacaaccttt ctgataccgt caatgcctgc ctgcgcctgc tcgatgcacc cgacaccgaa
    11461 atcgacgaac tgatcgacat tatccaagcc cccgacttcc cgaccggggc aaccatctac
    11521 ggcttgagcg gcgtgcgcga aggctataaa acaggccgcg gccgcgtcgt tatgcgcggt
    11581 aagacccata tcgaacccat aggcagaaac ggcgaacgcg aagccatcgt tatcgacgaa
    11641 atcccctatc aggtcaacaa agccaagctg gtcgagaaaa tcggcgattt ggttcgggaa
    11701 aaaacactgg aaggcatttc cgagctccgc gacgaatccg acaaatccgg tatgcgcgtc
    11761 gttatcgagc tgaaacgcaa cgaaaatgcc gaagtcgtct taaaccaact ctacaaactg
    11821 actccgctgc aagacagttt cggcatcaat atggtggttt tggtcgacgg acaaccgcgc
    11881 ctgttgaacc tgaaacagat tctctccgaa ttcctgcgcc accgccgcga agtcgttacc
    11941 cgacgtacgc ttttccggct gaagaaggca cgccatgaag ggcatatcgc cgaaggcaaa
    12001 gcggtcgcac tgtccaatat cgatgaaatc atcaagctca tcaaagaatc gcccaacgca
    12061 gccgaggcca aagacaaact gcttgcgcgt ccttggcgca gcagcctcgt tgaagaaatg
    12121 ctgacgcgtt ccggtctgga tttggaaatg atgcgtccgg aaggattggc tgcaaacatc
    12181 ggcttgaaag agcaaggtta ttacctgagc gagattcagg cagatgctat tttacgcatg
    12241 agcctgcgaa acctgaccgg cctcgatcaa gaagaaattg tcgaaagcta caaaaacctg
    12301 atgggtaaaa tcatcgactt tgtggatatc ctctccaaac ccgaacgcat tacccaaatc
    12361 atccgcgacg aactggaaga aatcaaaacc aactatggcg acgaacgccg cagcgaaatc
    12421 aacccgttcg gcggcgacat tgccgatgaa gacctgattc cgcaacgcga aatggtcgtt
    12481 accctgacac atggcggcta tatcaaaacc cagccgacca ccgactatca ggcgcagcgt
    12541 cgcggcgggc gcggcaaaca ggcggctgcc accaaagacg aagactttat cgaaaccctg
    12601 tttgttgcca acacgcatga ttatttgatg tgctttacca atttgggcaa gtgtcattgg
    12661 attaaggttt acaaactgcc cgaaggcgga cgcaacagcc gcggccgtcc gattaacaac
    12721 gtcatccagt tggaagaagg cgaaaaagtc agcgcgattc tggcagtacg cgagttcccc
    12781 gaagaccaat acgtcttctt cgccaccgcg cagggaatgg tgaaaaaagt ccaactttcc
    12841 gcctttaaaa acgtccgcgc ccaaggcatt aaagccatcg cgctcaaaga aggcgactac
    12901 ctcgtcggcg ctgcgcaaac aggcggtgcg gacgacatca tgctgttctc caacttaggt
    12961 aaagccatcc gcttcaacga atactgggaa aaatccggca acgacgaagc ggaagatgcc
    13021 gacatcgaaa ccgaaatttc agacggcatc gaagatgaaa ccgccgacag cgaaaacgca
    13081 ctgccgagcg gcaaacacgg tgttcgcccg tccggtcgcg gcagcggcgg tttgcgcggt
    13141 atgcgcctgc ctgccgacgg caa
//
LOCUS       DAWHSK010000031        12837 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.031, whole genome shotgun sequence.
ACCESSION   DAWHSK010000031 DAWHSK010000000
VERSION     DAWHSK010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12837
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.031"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..312)
                     /locus_tag="WIC03_000604"
     CDS             complement(<1..312)
                     /locus_tag="WIC03_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEZ3883145.1"
                     /translation="MYLPSMKHSLPLLATLVLAACSSTNTLPAGKTPADNIETADLSA
                     SVPTRPAEPEGKTLADYGGYPSALDAVKQKNDAAVAAYLENAGDSAMAENVRNEWLKS
                     LG"
     gene            843..1580
                     /locus_tag="WIC03_000605"
     CDS             843..1580
                     /locus_tag="WIC03_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEZ3883146.1"
                     /translation="MIILDKVSKHYQTRDKTRFAAVEPTSLEIRDGEIFGLMGYSGAG
                     KSTLLRLINLLERPDSGKVNVCGQELTALDAAALRQARQNIGMVFQQFNLLNNRTVAG
                     NVAFPLEIAGWPSEKIKARVAECLEIVGLTERAGHYPAQLSGGQKQRVGIARALAPKP
                     QVILADEPTSALDPATTRSVLECLEDINKRFNVTIVIVTHEMSVIRRLCDRAALLDKG
                     KVVEIVEVRGNQIHARSDIGRELIRED"
     gene            1583..2269
                     /locus_tag="WIC03_000606"
     CDS             1583..2269
                     /locus_tag="WIC03_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ3883147.1"
                     /translation="MAGLTFEQTVSNIIGMKGEIVRALGETFVMVGLSTTFAVVFGTL
                     LGVLLFVTSSRQLHYNKPVNFLLDNLVNLMRAFPFVILMIAMIPATRAIVGSTIGPVA
                     ASLVLSVSGLFYFARLVEQNLREVPKGVIEAAAAMGAPPVAIVCKVLLNEARAGMVSS
                     ITVLAIGLLSYSAAAGMIGGGGLGDLAIRYGYYRYQTEVIVFIVALLVLLVILIQSTG
                     NALARKLDKR"
     gene            2428..3291
                     /locus_tag="WIC03_000607"
     CDS             2428..3291
                     /locus_tag="WIC03_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883148.1"
                     /translation="MKTFFKTLSAAALALILAACGGQKDSAPAASASAAADNGAEKKE
                     IVFGTTVGDFGDMVKEQIQAELEKKGYTVKLVEFTDYVRPNLALAEGELDINVFQHKP
                     YLDDFKKEHNLDITEVFQVPTAPLGLYPGKLKSLEEVKDGSTVSAPNDPSNFARVLVM
                     LDELGWIKLKDGINPLTASKADIAENLKNIKIVELEAAQLPRSRADVDFAVVNGNYAI
                     SSGMKLTEALFQEPSFAYVNWSAVKTADKDSQWLKDVTEAYNSDAFKAYAHKRFEGYK
                     SPAAWNEGAAK"
     gene            complement(3353..3922)
                     /locus_tag="WIC03_000608"
     CDS             complement(3353..3922)
                     /locus_tag="WIC03_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEZ3883149.1"
                     /translation="MVRRLIIGISGASGFQYGVKALELLRAQDIETHLVVSKGAEMAR
                     ASETAYARDEVYALADFVHPIGNIGACIASGTFKTDGMLVAPCSMRTLASVAHGFGDN
                     LLTRAADVVLKERRRLVLMVRETPLNLAHLDNMKRVTEMGGVVFPPVPAMYRKPQTAD
                     DIVAHSVAHALSLFGIDTPDSAEWQGMAD"
     gene            4080..4940
                     /locus_tag="WIC03_000609"
     CDS             4080..4940
                     /locus_tag="WIC03_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEZ3883150.1"
                     /translation="MAKVKGGLGRGLDSLLANGADNSSGDRLTTVAVKDIRPGRYQAR
                     VQIDDEALQELADSIKAQGVIQPVIVREHGLSRYELIAGERRWRAAQIAGLTEIPAVI
                     KTISDETALAMGLIENLQRENLNPIEEAQGLKRLADEFGLTHETIAQAVGKSRSAISN
                     SLRLLSLPEPVQEMLYQRRLEMGHARALLTLPVVEQLELAQKAVKNGWSVREVERRSQ
                     AALQNKRPEPKKTAAADIGRLNDLLTEKLGVNAEIKTANHKKGRIILHFDTPETFDHI
                     LKQLGIDYRP"
     gene            5076..5414
                     /locus_tag="WIC03_000610"
     CDS             5076..5414
                     /locus_tag="WIC03_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883151.1"
                     /translation="MNTRIIVSAAFVALALAGCGSINNVTVSDQKLQERAAFALGVSP
                     NAVKISNRNNEGIRINFTATVGKRVSQCYVTSVISTIGVTTSDAICLGGGTHKGKSQC
                     NALLKAAGRC"
     gene            5782..6135
                     /locus_tag="WIC03_000611"
     CDS             5782..6135
                     /locus_tag="WIC03_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HEZ3883152.1"
                     /translation="MKQIIILQSAVLSICAAVAFAVWGFAGFLSAVGGGLSYLLPTFV
                     AVLLLKLFRGNPFLQSRMFVFGEILKVVLSLLSMLAVFAIWHQSLVFAPFLMGLLGVS
                     HLVFLVLLRVKDYGR"
     gene            6125..6991
                     /gene="atpB"
                     /locus_tag="WIC03_000612"
     CDS             6125..6991
                     /gene="atpB"
                     /locus_tag="WIC03_000612"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEZ3883153.1"
                     /translation="MAGETITAADYIKHHLQSLTSLSDVTQGQGLKNIADFSFINLDA
                     VFFAVLLGVIGSFLLWRGAKKATAGVPGRFQAAVEILFEFVDDMCKSIIHNEKSRKAV
                     APLGLTLFVWIFLMNAMDMLPVDLLPMVWQGITGNHHALLRVVPTADLNTTLALAVGV
                     LLICIYYNIKIKGLGGWFHELFSAPFGAKLAPANFLLNLVEFLSKTVSHGMRLFGNMY
                     AGELVFLLIALLGGAWAASGSVEVMDPILFVFHIIAGLAWAIFHILVITLQAFIFMAL
                     AFVYIGQAHDAH"
     gene            7048..7284
                     /gene="atpE"
                     /locus_tag="WIC03_000613"
     CDS             7048..7284
                     /gene="atpE"
                     /locus_tag="WIC03_000613"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEZ3883154.1"
                     /translation="MGLIAIACGLIVALGALGASIGIAMVGSKYLESSARQPELIGPL
                     QTKLFLIAGLIDAAFLIGVAIALLFAFVNPFAGA"
     gene            7355..7825
                     /locus_tag="WIC03_000614"
     CDS             7355..7825
                     /locus_tag="WIC03_000614"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEZ3883155.1"
                     /translation="MNINATLFAQIIVFFGLVWFTMKFVWPPIAKALDERAAKVAEGL
                     AAAERGKSDFEQAEKKVAELLAEGRNQVSEMVANAEKRAAKIVEEAKEQASSEAARIA
                     AQAKADVEQELFRARESLREQVAVLAVKGAESILRSEVDASKHAKLLDTLKQEL"
     gene            7830..8363
                     /locus_tag="WIC03_000615"
     CDS             7830..8363
                     /locus_tag="WIC03_000615"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEZ3883156.1"
                     /translation="MAEFATIARPYAKALFGLAQEKNQIESWLGGLEKLAAVVQEGKV
                     ASLIDRPETNASEKADILIDLVGLKDKELKNFVIVLAGQKRLSILPEVYAQYQDLTLS
                     FNHIKSAVIYSAYPLTDKQVGELAQMLNKRFDSELKISVEIEPELIGGIKVEVGDQVL
                     DLSVQGKLSALYTTMTN"
     gene            8374..9921
                     /locus_tag="WIC03_000616"
     CDS             8374..9921
                     /locus_tag="WIC03_000616"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEZ3883157.1"
                     /translation="MQLNPAEISDLIKAKIENLSVNAEVRTRGTVISVTDGIVRIHGL
                     SDAMQGEMLEFPGNTFGLAMNLERDSVGAVVLGEYEHIKEGDTVTCTGRILEVPVGRE
                     LVGRVVDALGRPIDGKGPINTTLTAPIEKIAPGVIARKSVDQPMQTGLKAIDSMVPVG
                     RGQRELIIGDRQTGKTAVALDAIVNQKGTGVICIYVAIGQKASSIANVVRKLEEHGAM
                     EHTIVVAATASEAAALQYIAPYSGCTMGEFFRDRGEDALIVYDDLSKQAVAYRQISLL
                     LRRPPGREAYPGDVFYLHSRLLERAARVNEHEVEKLTNGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETDLFNAGIRPAINAGISVSRVGGAAQTKVIKKLGGG
                     IRLALAQYRELAAFSQFASDLDEATRKQLEHGEVVTELMKQKQFSTLNTAEMALTLWA
                     INNGSYSDVPVAKALAFESEFLSFVRTQHPEVLEAVNASGAMSDESEKTLEAAMKSFK
                     SSYAYQA"
     gene            9946..10821
                     /gene="atpG"
                     /locus_tag="WIC03_000617"
     CDS             9946..10821
                     /gene="atpG"
                     /locus_tag="WIC03_000617"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEZ3883158.1"
                     /translation="MAVGKEILTKIRSVQNTQKITKAMQMVSTSKMRKTQERMRLARP
                     YAEKVRMVMSHLAQTNTDHGIPLLESHREIRRVGFILITSDKGLCGGLNANVLKKFLA
                     QVQEYRNQGIEEEIVCLGSKGLMACQSIGLNVVASAVNLGDTPKMEMLLGPLTELFQR
                     YEKHEIDRIHLVYSGFVNTMRQEPRMEVLLPIGENVIGDSAPKSPFSWEYRYEPTALA
                     VLEYLVRRYLESVVYQALSDNMASEQAARMVAMKAATDNAGNAIKELRLVYNKSRQAA
                     ITTELSEIVAGAAAV"
     gene            10859..12256
                     /gene="atpD"
                     /locus_tag="WIC03_000618"
     CDS             10859..12256
                     /gene="atpD"
                     /locus_tag="WIC03_000618"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEZ3883159.1"
                     /translation="MSQGKIVQIIGAVVDVEFPRDMIPRVYDALKLDENGLTLEVQQL
                     LGDGVVRAIAMGSSDGLKRGMTVSNTGAPITVPVGKGTLGRIVDVLGTPVDEAGPIDT
                     DKSRAIHQAAPKFDELSSTTELLETGIKVIDLLCPFAKGGKVGLFGGAGVGKTVNMME
                     LINNIAKAHSGLSVFAGVGERTREGNDFYHEMKDSNVLDKVAMVYGQMNEPPGNRLRV
                     ALTGLTMAEYFRDEKDENGKGRDVLFFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTL
                     AEEMGRLQERITSTQTGSITSIQAVYVPADDLTDPSPATTFAHLDATVVLSRDIASLG
                     IYPAVDPLDSTSRQLDPMVLGQEHYDVARGVQSTLQKYKELRDIIAILGMDELSDEDK
                     LTVMRARKIQRFLSQPFHVAEVFTGSPGKYVALRDTIAGFKAILNGEYDHLPEQAFYM
                     VGSIEEAVEKAKTLN"
     gene            12267..12689
                     /locus_tag="WIC03_000619"
     CDS             12267..12689
                     /locus_tag="WIC03_000619"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEZ3883160.1"
                     /translation="MSIMQVEVVSSEQNIYSGEASFVVVPTVQGELGIYPRHEPIMSL
                     VRPGALRLTVPGEDKEVLVAVSGGVLEVQPDKVTILADVAVRSAEMDQARAEEAKKAA
                     EAGISQAKDDKALAEAHKALAAAIAQLKTLDYIRSHKK"
BASE COUNT         3133 a         3278 c         3326 g         3100 t
ORIGIN      
        1 gcccaaagac ttcagccact cgttgcggac attttccgcc atcgcactgt cgccggcgtt
       61 ttccaaatag gcggcgacgg cggcatcgtt tttctgtttc actgcatcca gtgcggacgg
      121 gtagccgccg taatctgcca gcgtttttcc ttccggttcg gcagggcggg tgggaacgct
      181 tgccgaaagg tcggcagttt ctatattgtc tgccggggtc ttgccggctg gcagtgtgtt
      241 tgtcgaagaa cacgcggcaa gcactagggt cgccagcagc ggcagggaat gcttcataga
      301 gggtaggtac atcggatttc cttaagaatc ggaaccctga acggtcaggg ttggaaaaga
      361 caaaatgccg tctgaacagg cgtttgcccg aattatatgc cgaaactgca ccgcctttgg
      421 aatgtttccg acataattta tagtggatta aatttaaacc agtacagcgt tgcctcgcct
      481 tagctcaaag agaacgattc tctaaggtgc tgaagcacca agtgaatcgg ttccgtacta
      541 tttgtactgt ctgcggcttc gtcgccttgt cctgattttt gttaatccac tatatatttt
      601 tcaattattt gccgttttgg tgcgaaccgc tgcctttgcc cgtttcagac ggcattgtcc
      661 gaaatggttg cctgcttcct gccttattga caaaaaaacg ctttcccgat aatatcctac
      721 gaaaattaac ctgccgattt gacacagctt gcgggcataa cagctaaagc gttccgacaa
      781 tttcagcttt atcttccgcg cccgttgtgt ccgacatcgg gctttgttgt atgggaaaga
      841 caatgattat tttggacaag gtttccaagc attaccaaac gcgcgacaag acccgttttg
      901 ccgccgtcga gccgaccagc ctcgaaatcc gcgacggcga aatcttcggg ctgatgggtt
      961 attcgggtgc aggcaaatcc accctgttgc gcctgattaa cctgttggaa cgtcccgaca
     1021 gcggcaaggt caacgtctgc ggacaagagc tgaccgcgct cgatgccgcc gcattgcgtc
     1081 aggctcggca gaatatcggc atggtgtttc agcagttcaa cctgttgaac aaccgcaccg
     1141 tcgccggcaa cgtcgccttc ccattggaaa tcgccggatg gccgtctgaa aaaatcaaag
     1201 cgcgcgttgc cgaatgtctc gaaatcgtcg gactgaccga acgcgccggc cactatcccg
     1261 cccagctttc aggcgggcag aaacagcgtg tcggcatcgc ccgcgcactc gcgcccaaac
     1321 cccaagtcat cctcgccgac gaacccactt ccgccctcga ccccgccacc acgcgcagcg
     1381 tcttggaatg tttggaagac atcaacaaac gattcaacgt aaccatcgtc atcgtaaccc
     1441 acgaaatgag cgttatcaga cgactgtgcg accgcgccgc cctgttggat aaaggcaaag
     1501 tcgtcgaaat cgtcgaagta cgcggcaacc aaatccacgc ccgatccgac atcgggcgcg
     1561 aactgattcg ggaggactga atatggcagg cttaacattt gaacaaaccg tttccaacat
     1621 catcggtatg aaaggcgaaa tcgtccgcgc attgggcgaa accttcgtaa tggtcggttt
     1681 gtccaccaca ttcgccgtcg ttttcggcac gttgctgggc gtgttgctct tcgtaacttc
     1741 cagccgccaa ctgcattaca acaagccggt gaacttcctg ctcgacaacc tcgtcaacct
     1801 catgcgcgcc ttccccttcg tcattttgat gattgcgatg atacccgcca cacgcgccat
     1861 cgtcggcagc accatcggcc cggttgccgc ctcgctggtg ttgagcgtgt cgggattgtt
     1921 ttattttgcc cgactggtgg aacaaaacct gcgcgaagtc cccaaaggcg tgattgaagc
     1981 cgccgccgcg atgggtgcgc cgccggttgc catcgtctgc aaagtcctct tgaacgaagc
     2041 gcgcgcgggc atggtttcca gcattaccgt gcttgccatc gggcttttgt catacagcgc
     2101 ggcggcaggg atgataggcg gcggcggctt gggcgacctc gccatccgct acggctacta
     2161 ccgctaccaa accgaagtca tcgtcttcat cgtcgccctc ctcgtgctgc tggtcatcct
     2221 gattcaaagc accggcaacg cattggcgcg gaaattggat aaacgttaaa cccgaatgcc
     2281 gtctgaacgc caaaaccccc accgctatcc gaaaaatgct ataaaatccc cctgttcgcg
     2341 gcaaatgccg tctgaacgcc gaatctggac ggcaggactc cctgcccgtc atttttgttt
     2401 gaaactgcca caacatcagg agaaaatatg aaaaccttct tcaaaaccct ttccgccgcc
     2461 gcactcgcgc tcatcctcgc cgcctgcggc ggtcaaaaag acagcgcgcc cgccgcatcc
     2521 gcttctgccg ccgccgacaa cggcgcggag aaaaaagaaa tcgtcttcgg cacgaccgtc
     2581 ggcgacttcg gcgatatggt caaagaacaa atccaagccg agctggagaa aaaaggctac
     2641 accgtcaaac tggtcgagtt taccgactat gtgcgcccga atctggcatt ggctgagggc
     2701 gagttggaca tcaacgtctt ccaacacaaa ccctatcttg acgacttcaa aaaagaacac
     2761 aatctggaca tcaccgaagt cttccaagtg ccgaccgcgc ctttgggact gtacccgggc
     2821 aagctgaaat cgctggaaga agtcaaagac ggcagcaccg tatccgcgcc caacgacccg
     2881 tccaacttcg cccgcgtctt ggtgatgctc gacgaactgg gttggatcaa actcaaagac
     2941 ggcatcaatc cgctgaccgc atccaaagcg gacattgccg aaaacctgaa aaacatcaaa
     3001 atcgtcgagc ttgaagccgc gcaactgccg cgtagccgcg ccgacgtgga ttttgccgtc
     3061 gtcaacggca actacgccat aagcagcggc atgaagctga ccgaagccct gttccaagaa
     3121 ccgagctttg cctatgtcaa ctggtctgcc gtcaaaaccg ccgacaaaga cagccaatgg
     3181 cttaaagacg taaccgaggc ctataactcc gacgcgttca aagcctacgc gcacaaacgc
     3241 ttcgagggct acaaatcccc tgccgcatgg aatgaaggcg cagccaaata aggcagtcgt
     3301 ataaaatgat gccgtctgaa ccgtatccgt gttcagacgg catttttgtc ctttaatccg
     3361 ccattccctg ccattccgcc gaatccggcg tatcgattcc gaacagcgac aaagcgtgtg
     3421 caacactgtg cgccactatg tcgtccgccg tctgcggttt gcggtacatc gcaggaacag
     3481 ggggaaacac cacgccgccc atttccgtta cccgcttcat attgtccaaa tgggcaaggt
     3541 tcagcggcgt ttcgcgcacc atcagcacca gccgccgcct ttccttcaaa accacatccg
     3601 ccgcacgcgt caagaggttg tcgccgaagc cgtgcgcgac cgaggcaagc gtccgcatcg
     3661 aacagggggc gaccagcatc ccgtccgttt taaacgtacc gctggcaatg cacgccccga
     3721 tattgccgat cggatgcacg aagtccgcga gggcatatac ctcgtctctc gcataagccg
     3781 tttccgaagc gcgcgccatc tccgcacctt tcgataccac aaggtgcgtt tcgatatctt
     3841 gcgcgcgtaa aagttccaaa gccttcacgc cgtattggaa accgctcgcc ccactgatgc
     3901 cgattatcaa acgccgtacc atcatccgcc tttcccataa aaccgcctgc aacggcaaac
     3961 cggctattat agtgaaaaaa cagaaatccg ataaatacgg atacaaattg tcggcaacac
     4021 ccaatatccg ataaaatatc cgatttaaca tcctatctga ataggcacgg gagggcggta
     4081 tggcaaaagt aaaaggcgga ttggggcgcg gtttggattc gctgctcgcc aacggcgcag
     4141 acaacagcag cggcgaccga ttgaccacgg ttgcggttaa agatatccgg cccggccgct
     4201 atcaggcgcg tgttcaaatc gatgacgaag ccttgcagga gctggcagat tcgattaagg
     4261 cgcaaggcgt gatacagccc gtcatcgtgc gcgaacacgg actgtcccga tacgaactga
     4321 ttgcggggga gcggcgttgg cgtgccgcac agattgccgg cctgaccgaa atccccgccg
     4381 ttatcaaaac catcagcgac gaaaccgcgt tggcaatggg tttgatcgaa aacctccagc
     4441 gcgaaaacct caaccccatc gaagaagcac aaggcttgaa acgccttgcc gacgagttcg
     4501 ggctgaccca cgaaaccatc gcccaagccg tcggtaaaag ccgaagcgcg atttccaaca
     4561 gcctgcgcct tttaagcctg cccgaacccg tgcaggaaat gctttaccaa cgccgcctcg
     4621 aaatggggca cgcccgcgca ctgctgaccc tgcccgtcgt cgaacagctc gaattggcgc
     4681 aaaaggctgt caaaaacggc tggtcggtgc gcgaagtcga acgccgcagc caagccgccc
     4741 ttcaaaacaa acgtcccgag cccaaaaaaa ctgccgccgc cgacatcggc cgcctgaatg
     4801 atttgctgac tgaaaaactg ggtgtcaacg ccgaaatcaa aaccgccaac cataaaaaag
     4861 gcagaatcat cctgcatttc gacacgcccg aaaccttcga ccatatcttg aagcaattgg
     4921 gcatagatta ccgaccttaa ttttgcggga tataccgtct gaaatataga gaatagcttt
     4981 ccagatttta agtgggaaat ataattctat tgacattttt tctgcttcac gtaagaatcg
     5041 ttttcctgtt ttcattttta attttcgagg aaattatgaa cacacgcatc atcgtttcgg
     5101 ctgcgttcgt tgcgttggca ttagcaggtt gcggctcaat caataatgta accgtttccg
     5161 accagaaact tcaggaacgt gccgcgtttg ccttgggcgt cagcccaaat gccgtaaaaa
     5221 tcagcaaccg caacaatgaa ggcatacgca tcaactttac cgcaactgtg ggtaagcgcg
     5281 tgagccaatg ctatgttacc agtgtaatca gcacaatcgg cgttaccact tccgatgcaa
     5341 tttgtttggg aggcggaacg cacaaaggca aaagtcaatg caatgctttg cttaaagcgg
     5401 caggccgttg ctaatccttt attcggaaaa ggtcgtctga aaatattttc agacgacctt
     5461 ttatttattg agcaaattcc ccaaactgcc gacaatccga ccgataaact gctacaattt
     5521 cgcgttgatt ttttattatt tccacgtaaa gcaaacaaat cacgtaagaa cattaaaagt
     5581 gtaaagaaat tgcggacaaa ctccttaata tcctgtaatt atatgtaatg tgtgttagaa
     5641 tgacacctgt cgccgttatt caggcggcga gcgcggcgga ttgggatacg ttgtttcaca
     5701 aagattaact gtcgcttgac gtaataaggc ttctaaatta tattagccgc gccccaaatc
     5761 cgaggcggaa acattccctt tatgaagcag attatcatcc tacaatccgc cgtattgtcc
     5821 atatgcgccg cagttgcctt tgctgtatgg ggttttgccg gattcctttc ggcggtcggt
     5881 ggcggtttgt cctacctgct tcccacgttt gttgcagttt tacttttaaa acttttcagg
     5941 ggaaaccctt tcctgcaaag caggatgttc gtcttcggag agattttaaa agtagtgctg
     6001 tcgctgttgt ccatgctcgc cgtatttgca atatggcatc aatcgctggt gttcgccccg
     6061 tttctgatgg ggctgctcgg tgtcagccat ttggtttttt tagtattgtt gagagtgaaa
     6121 gattatggca ggtgaaacca ttaccgctgc cgactacatc aagcaccact tgcagagctt
     6181 gaccagtttg tcggatgtta ctcagggtca gggactgaaa aacattgctg atttttcgtt
     6241 tattaacctt gatgccgtct tttttgccgt cctgttgggc gtaatcggca gcttcctgtt
     6301 gtggcgcggt gcgaaaaaag caacggcagg cgttcccgga cgtttccagg ccgcagtgga
     6361 aatcttgttt gagtttgtgg acgatatgtg taagagcatc attcacaacg aaaagtctag
     6421 aaaagccgtc gcgccgttgg gtctgacgct gtttgtctgg atttttctga tgaacgcgat
     6481 ggatatgctg ccggtcgatt tgctgccgat ggtatggcag ggcattaccg gcaaccatca
     6541 cgccctgctg cgcgtcgtac cgactgccga tttgaacacc actttggcac tcgctgtcgg
     6601 cgtgttgctg atttgtatct attacaacat caaaatcaaa ggattgggtg gctggtttca
     6661 cgagttgttc agcgcgccgt tcggtgcaaa actcgcacct gcgaacttcc tgttgaattt
     6721 ggtcgagttt ctttctaaaa ccgtatccca cggtatgcgg ttgttcggca atatgtatgc
     6781 gggcgaattg gtattcttgc tgatagcctt gcttggcggt gcttgggcgg cttccggcag
     6841 cgtcgaagtc atggatccga ttctgtttgt attccacatt attgccggtt tggcatgggc
     6901 gattttccat attttggtga ttaccctgca ggcgtttatt ttcatggcgt tggcgttcgt
     6961 ctatatcgga caggcacatg atgcgcactg attttcctaa gtagtttgtt tttgtagttt
     7021 aacctttgtt tctttaagga gtttaaaatg ggtttgattg ctatcgcatg tggtttgatc
     7081 gttgcattgg gtgcattggg tgcctctatc ggtatcgcaa tggtcggttc taaatatttg
     7141 gagtcttctg cccgtcagcc cgaactgatc ggtccgctgc aaaccaaact gttcctgatt
     7201 gccggtttga ttgatgccgc attcttgatt ggtgtcgcca ttgcactgct gttcgccttc
     7261 gttaacccgt ttgcaggtgc ataatcagga cggattcgtc cgttccgtac aggcgcagcc
     7321 tgtttgatta accccagtac gaaggttaag taacgtgaat atcaatgcaa cattattcgc
     7381 tcaaatcatc gtctttttcg gtttggtatg gtttaccatg aaattcgtgt ggccgccgat
     7441 tgcaaaagct ttggatgagc gtgccgcaaa agtcgccgaa ggcttggctg ccgccgagcg
     7501 tggtaaaagc gatttcgagc aggctgaaaa aaaggttgca gaactcttgg cagaagggcg
     7561 taatcaggtt tccgaaatgg ttgccaacgc cgaaaaacgt gccgccaaaa ttgtcgaaga
     7621 agccaaagaa caggcttctt ccgaggcggc gcgcattgca gctcaggcaa aggccgatgt
     7681 ggagcaggaa ttgttccgcg cacgcgaatc cctgcgcgag caggttgccg tgttggctgt
     7741 caaaggtgcc gaatctattt tgcgcagcga agtcgatgct tccaaacacg caaaactgct
     7801 cgataccctg aaacaggagc tgtaatctta tggcagagtt cgcaacgatt gccagacctt
     7861 atgcaaaggc attgttcggt ctggctcagg aaaaaaacca aattgagtct tggttgggcg
     7921 gactggaaaa acttgcggcg gttgttcagg aagggaaggt ggcttcattg attgaccgtc
     7981 ctgaaacgaa tgcttcagaa aaagcagata tcctcatcga tttggtcggt ttgaaagaca
     8041 aggagttgaa aaactttgtt attgtcttgg ccgggcagaa acgtttgtcg atattgccgg
     8101 aagtatatgc tcaatatcaa gacttgacct tatcattcaa ccatatcaaa tctgccgtca
     8161 tttacagtgc ctatccgttg accgacaaac aggtcggcga gttggcgcaa atgctgaata
     8221 agcgtttcga cagcgagctg aaaatctctg tcgaaatcga accggagctg attggcggca
     8281 taaaagttga agtgggtgat caggttttgg atttgtctgt acaaggcaaa ttgagtgcct
     8341 tgtacacgac tatgacgaat taggagagtt ttcatgcagc ttaatcctgc tgaaattagc
     8401 gatttgatta aagccaagat cgaaaatctg tctgtaaatg ccgaagtgcg tacccgtggt
     8461 acggtgattt cggtaactga cggtatcgtt cgcatccatg gtttgtcaga tgcaatgcaa
     8521 ggtgagatgc tcgaattccc gggtaacact ttcggcttgg cgatgaacct ggagcgcgac
     8581 tccgtcggcg ccgtagtgtt gggcgagtac gaacatatta aagaaggcga cacggttact
     8641 tgtaccggtc gtatcttgga agtgccggtc ggacgcgaac tggtcggacg cgtcgttgat
     8701 gcattgggtc gtccgattga cggcaaaggc ccgattaata caactttgac cgcccctatc
     8761 gaaaaaatcg caccgggcgt gattgcgcgc aaatcggttg accagccgat gcaaaccggt
     8821 ctgaaggcga ttgactctat ggttcctgtc ggtcgcggtc agcgtgagtt gattattggc
     8881 gaccgtcaga caggtaaaac ggccgtagca ttggatgcca tcgtcaacca aaaaggtacg
     8941 ggcgttatct gtatctatgt cgctatcggt caaaaagcat cttctattgc caacgtggtc
     9001 cgcaaattgg aagagcatgg cgcgatggaa cacaccatcg tggttgctgc aactgcatct
     9061 gaagcggcgg cattgcaata tatcgcacct tactccggtt gtacgatggg tgaattcttc
     9121 cgcgaccgcg gcgaagatgc cttgattgtt tatgacgatt tgtccaaaca ggctgtggct
     9181 taccgtcaaa tttccctgct tttgcgccgt ccgcccggcc gcgaagccta tcccggcgat
     9241 gtgttctacc tgcattcccg tctgttggaa cgtgcagcac gtgtcaatga acacgaagtg
     9301 gagaaattga ccaacggcga agtaaaaggc aaaaccggtt ctctgaccgc gttgccgatt
     9361 atcgaaaccc aagcgggcga cgtatctgct ttcgttccga ctaacgtcat ttcgattacc
     9421 gacggtcaga ttttcttgga aaccgacctc ttcaacgccg gtatccgtcc tgcaatcaat
     9481 gccggtattt ccgtatcccg cgtaggcggt gctgcacaaa ccaaagtgat taaaaagctg
     9541 ggtggcggta tccgtttggc gttggcgcaa tatcgtgaat tggcggcttt ctcgcaattt
     9601 gcatccgatt tggatgaagc tacgcgcaaa caattggagc atggcgaagt cgtaaccgaa
     9661 ctgatgaaac agaaacagtt cagtacgttg aatacggctg aaatggcttt gaccctttgg
     9721 gcaatcaaca acggttcgta ttctgatgtt ccggttgcca aagctctggc ttttgaatct
     9781 gaatttttga gctttgtccg tacccaacat ccggaagttt tggaagccgt caatgcttca
     9841 ggtgcaatgt ccgacgagag cgagaaaacg cttgaagcag ccatgaaatc cttcaaatct
     9901 tcttacgcct atcaggcata aggcttgaag tgaaaggagt ctgaaatggc agtaggaaaa
     9961 gagattctca ccaaaatccg cagtgttcag aatacccaaa agatcactaa agcgatgcag
    10021 atggtgtcaa cctctaaaat gcggaagact caggaacgga tgcgtttggc gcgtccgtat
    10081 gccgaaaaag tgcgtatggt gatgagccat cttgcgcaaa ccaataccga tcatggtatt
    10141 ccgttactgg aatctcatcg ggaaatcaga cgtgtcggtt ttattttgat tacgtctgat
    10201 aagggtttgt gtggcggttt gaacgccaac gtgctgaaaa agtttttggc acaagttcaa
    10261 gagtatcgga atcaaggtat tgaagaggaa atcgtatgcc ttggcagtaa aggtctgatg
    10321 gcgtgtcaga gcattggtct gaatgtggtt gccagtgccg taaatttggg cgatacccca
    10381 aaaatggaaa tgctgctcgg acctttgaca gaactcttcc aacggtatga gaaacatgaa
    10441 attgacagaa tccatctagt gtattcgggt tttgtcaata ccatgcgtca agaaccgaga
    10501 atggaagtat tgctgcctat cggtgagaac gtgattggcg attcagctcc caaatcaccg
    10561 ttcagctggg aataccgcta cgaaccgact gcacttgcag tgttggaata tctggttcgc
    10621 cgctatttag agtctgtggt ttatcaggcg ttgagcgaca atatggcatc cgaacaggca
    10681 gcccgcatgg ttgccatgaa agctgcaaca gacaatgcag gcaatgccat caaagagttg
    10741 cgtttggtat ataacaaatc gcgtcaagct gcgattacca cggaattgtc agaaattgta
    10801 gcaggtgcgg cggctgtctg atgccgtctg aatcctgtac ggaaattagg atacgataat
    10861 gagccaaggc aaaatcgtac aaattatcgg tgcggttgtt gacgtggaat ttccacgcga
    10921 catgattccg cgcgtttacg acgctttgaa attagacgaa aacggtctga ctttggaagt
    10981 ccaacagctt ttgggcgacg gcgtagtccg tgccattgcg atgggcagct cggacggttt
    11041 gaaacgcggc atgactgtga gcaataccgg tgcgcccatt actgtgccgg taggtaaagg
    11101 tacgttggga cgcattgtcg atgtattggg aacgcctgtt gacgaagcag gtccgatcga
    11161 taccgacaag agccgtgcca tccaccaagc tgcccctaag tttgacgaac tgtcttccac
    11221 aaccgaattg ctcgaaacgg gcattaaagt gattgacttg ctgtgtccgt ttgccaaagg
    11281 cggtaaagta ggtctgttcg gcggtgccgg tgtgggtaaa accgtgaaca tgatggaatt
    11341 gatcaacaac atcgccaaag cgcacagcgg cttgtccgtg ttcgcaggcg tgggtgagcg
    11401 tacccgcgaa ggtaacgact tctaccacga gatgaaagat tccaacgtat tggataaagt
    11461 ggcaatggtt tacggtcaga tgaacgaacc tccgggcaac cgtttgcgcg tcgcattgac
    11521 cggtttgacc atggcggaat acttccgtga cgaaaaagac gaaaacggca aaggccgcga
    11581 cgtattgttc ttcgtggaca acatctaccg ttacaccctg gctggtaccg aagtatccgc
    11641 attgttgggt cgtatgccgt ctgcagtggg ctaccaaccg acattggcag aagaaatggg
    11701 tcgtttgcag gagcgtatta cctctaccca aaccggttct attacttcca tccaagccgt
    11761 atatgtacct gcggacgact tgaccgaccc gtctccggca acaacgttcg cccacttgga
    11821 cgcaaccgtc gtattgagcc gcgatattgc ctctttgggt atttacccgg cagtcgatcc
    11881 gcttgattct acttcgcgcc aactggatcc gatggtattg ggtcaagagc actacgacgt
    11941 agcgcgcggc gtacagtcca ccctgcagaa atacaaagaa ttgcgcgaca tcatcgccat
    12001 cttgggtatg gacgaattgt ctgacgaaga caaactgacc gtaatgcgtg cgcgtaaaat
    12061 ccaacgcttc ctgtcccaac cgttccacgt tgccgaagtg tttacaggtt ctccgggcaa
    12121 atatgtcgcc ctgcgcgata ccattgccgg cttcaaagcc atcttgaacg gcgaatacga
    12181 ccatctgccc gaacaggcat tctacatggt cggcagcatc gaagaagcgg ttgagaaagc
    12241 gaaaacctta aactgaggag gtcggcatga gcatcatgca agttgaggtg gtcagtagcg
    12301 agcaaaacat ctattcgggc gaagccagct ttgtagtggt tccgaccgtt caaggtgagc
    12361 ttggtattta cccgcgacac gagccgatta tgagtttggt gcggccgggg gctttgcgtt
    12421 tgaccgttcc gggcgaggat aaagaggttt tggttgctgt ttccggcggt gttttggaag
    12481 tacagcctga taaagtaact atcttggcgg atgttgccgt ccgcagtgcg gagatggatc
    12541 aggcacgtgc ggaagaggcg aaaaaagccg cagaagcggg catttcccaa gctaaagacg
    12601 ataaggcttt ggcggaggcg cataaagcat tggctgctgc gattgcacag ctcaaaactt
    12661 tggactatat ccgttcgcac aagaaataaa cttgtttggt ttggaaaagc acgaccgttt
    12721 ggtcgtgctt tttattttcc ggatttaaat taaagactgt gtttggatat ggcgttggca
    12781 ggcatagcct gctttaaggt attcaatagg gcagcctgcc gcctaaccga aacatat
//
LOCUS       DAWHSK010000032        12712 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.032, whole genome shotgun sequence.
ACCESSION   DAWHSK010000032 DAWHSK010000000
VERSION     DAWHSK010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12712
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.032"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..1173
                     /locus_tag="WIC03_000620"
     CDS             <1..1173
                     /locus_tag="WIC03_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEZ3883161.1"
                     /translation="TRKFAPFTAGSGIPQVIASLSLPYGAQKTRLIRLGQTLLKIPLT
                     FLGMLFGASIGREGPSVQVGAAVMGAWGAWCKKHGLAFKGMQENDLMAAGAAGGLAAA
                     FNAPLAGVIFAIEELGRGIMLRWERQILLGVLASGFIQVAIQGNNPYFSGFNGGVLEH
                     IFLWVALSGLVCGAAGGLFGRLLYRGAAAFAPRKIRGFIRNRPLLLAALMGLLLALLG
                     TFYQGKTYGTGYHEAAQALHGIYEAPFGLAAAKWLATVFSYWAGVPGGIFTPSLTIGA
                     VLGEHIAAIADISQGANIIVLICMAAFLAGATQSPITSAVVVMEMTGGQSLLFWMLIA
                     CIFASQVSRQFSPRPFYHASGMRFRQRVLQETTAQTGNAPARPQTANSKTGMPSEN"
     gene            1363..2754
                     /gene="hrpA"
                     /locus_tag="WIC03_000621"
     CDS             1363..2754
                     /gene="hrpA"
                     /locus_tag="WIC03_000621"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HEZ3883162.1"
                     /translation="MFPDFSQTLSKDRHFLRSAFKNPNKYGGLSKVEEKYRKSHEIFL
                     KRLAALPKPEFDNTLPVHEKLDEIKKTVAEHQVTIICGETGSGKTTQLPKICLELGRG
                     AAGLIGHTQPRRLAARSVAERIAEELKSEIGSAVGYKVRFTDHTSRDACVKLMTDGIL
                     LAETQTDRYLAAYDTIIIDEAHERSLNIDFLLGYLKQLLPRRPDLKVIITSATIDAER
                     FSQHFNGAPVLEVSGRTYPVEILYRPLIGKDEDDAEIELTDAIVDAADELARYGEGDI
                     LVFLPGEREIREAAEALRKSTLRRNNEILPLFARLSHAEQHKIFHPSGAKRRIVLATN
                     VAETSLTVPGIKYVIDTGLARVKRYSARAKVEQLHIEKISQAAARQRSGRCGRVSAGV
                     CIRLFSEENFNNRPEFTDPEIARSNLAAVILRMASLKLGDVAAFPFLEMPDSRYINDG
                     FQVLLELGAVEAA"
     gene            2855..3982
                     /locus_tag="WIC03_000622"
     CDS             2855..3982
                     /locus_tag="WIC03_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883163.1"
                     /translation="MKHLLIDFENVQPQNLDKLLTENTHIWLFIGVLHKMLPISLVQS
                     LLRFGERVHLVQLQKTGKNALDFYLSYYLGQITATDPNAQIGILSRDGGYDVLVEHIL
                     KNHQAKGIVRLANIDEVQHQKITTEPPSALLENTPQPETTLKPQQPLTSYFQAALTAL
                     RRPDAFRPCRLHNLRQNLRKHILSDLFKEKTDEECEITTANVINKLKAQNFISIDEQE
                     TVSYHLSDNDLLQRIQRHILSQRPKTYADFQAVVQNRADALHLTVGTNDIQSFARHLR
                     DQNLIRQNNGKIEYAPFTEPKPQPTPKQPKKTAWEPDEIIWKKVIAALSLKNRPNKTK
                     TLRNTIQALTKSNAQETDKLLQHLQDTQVLRIDGTKIVYIK"
     gene            4159..5736
                     /gene="lpt3"
                     /locus_tag="WIC03_000623"
     CDS             4159..5736
                     /gene="lpt3"
                     /locus_tag="WIC03_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipooligosaccharide phosphoethanolamine
                     transferase"
                     /protein_id="HEZ3883164.1"
                     /translation="MMKKSLFVLFLYSSLLTASEIAYRFVFGIETLPAAKMAETFALT
                     FVIAALYLFARYKTSRLLIALFFAFSIIANNVHYAVYQSWMTGINYWLMLKEVTEVGS
                     AGASMLDKLWLPALWGVAEVVLFCSLAKFRHKTHFSADILFAFLMLMIFVRSFDTKQE
                     HGISPKPTYSRIKANYFSFGYFVGRVLPYQLFDLSKIPVFKQPAPSRIGQGSIQNIVL
                     IMGESESAAHLKLFGYGRETSPFLTRLSQADFKPIVKQSYSAGFMTAVSLPSFFNVIP
                     HANGLEQISGGDTNMFRLAKEQGYETYFYSAQAENQMAILNLIGKKWIDHLIQPTQLG
                     YGNGDNMPDEKLLPLFDKINLQQGRHFIVLHQRGSHAPYGALLQPQDKVFGEADIVDK
                     YDNTIHKTDQMIQTVFEQLQKQPDGNWLFAYTSDHGQYVRQDIYNQGTVQPDSYLVPL
                     VLYSPDKAVQQAANQAFAPCEIAFHQQLSTFLIHTLGYDMPVSGCREGSVTGNLITGD
                     AGSLNIRDGKAEYVYPQ"
     gene            5838..9047
                     /locus_tag="WIC03_000624"
     CDS             5838..9047
                     /locus_tag="WIC03_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HEZ3883165.1"
                     /translation="MDARSNPANVSDGLQNSSSHIGTNTRYRLTKLGEQMARLPIDPK
                     IARILLAAKKHDCMAEILVIASALSIQDPRERPLEARDAASKAHERFTDKQSDFLAYL
                     NIWDSFQRERDKGLSNKQLVQWCRQYFLSHLRMREWRELHHQLAQTAIEMGLTTKEAA
                     FRRSPEVRQLTSSENAGDQDLSAKLKQKQLDKKQHRAQIRAAKEAGYEQIHRALLTGL
                     IANVGMKSPDGNDYTSTRGSRFHLFPASALFKAKPKWVMAAELVETTKLYARDVAAIQ
                     PEWIEQEAPHLVRYHYFEPHWEQKRGEVIASERVTLYGLTVLPRRPVSYGRIAPEEAR
                     EIFIRGALVAQECDLKADFFVHNKKLIKEITELEHKSRKQDVLVDDEALFAFYNERLP
                     EMAWKDAQGSVWGSEDSVRIIESDKAERSSENERNEFRKNKRNGSRQNENHGNTVGWV
                     ENPTSAATAKTVGFDNPTYATQQPTPSPEREGRGEGKTVAAQTNFSATAANPLPQERE
                     QSASASTFSDDLRPANLQQTAPSPVGEGWGESKTVATQTNFSATSTLSDDPKPKKQPA
                     PQKGRLKPLPLADIRTFQAWLKTAECDNPRLLFLSRDDLMQHAAAHITEEQFPKFWQT
                     ADGKFKLSYRFEPHHPLDGVTMTVPLTVLNRLHAPSLEWLVPGMLREKIQLLIKALPK
                     QIRRICVPVPDFITKFLESNPDRQATIIPQLAHFIAKSASDMRILEQIDQDAWAAQEL
                     PEHCYLNLRIIDDGGQELAGGRKLHELQQQLGQAAAVTFRDNTQEFERDNVTTWDIGT
                     LPESIKFARGKQQLTGYLGLQKEKDGRIALRLFDTTEAAEQAHRQGVIELMKLQLKEQ
                     VKDLNKGIQGFTQAAMLLKHINADTLRDDLTQAVCDRAFIGEDELPRNEKAFKEQIKR
                     ARSRLPAVKEALSRYLQETAAAYAELNGKLGKHPLTHLLRLRLQTLLAPGFATRTPWA
                     QWPRLPIYLKAMTLRLEKYSSNPARDAAREADIQELEQMWQEKNDGLVKQGLPISDGL
                     AAFKWMIEELRVSLFAQELKTPYPVSVKRLMKEWERLNK"
     gene            9106..10713
                     /locus_tag="WIC03_000625"
     CDS             9106..10713
                     /locus_tag="WIC03_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003758255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEZ3883166.1"
                     /translation="MKSENNYERIVQNSKKFQHSQKNEMQKRITDLLQQLTNGIHERD
                     QIMAVSLLGAIAGHNTFLFGPPGTAKSLISRRLACAFENPQYFEYLMNRFSTPEEVFG
                     PVSIKALKEDRYLRKIEGYLPTADFAFLDEIWKSSPAILNNLLTIINEHIFKNGDERF
                     NVPLKALIAASNEIPPENQGLEALYDRFIIRLLVPPIEQEENFNRLLDSKPSSDKPEI
                     SEDLRISYHELAKWREQLHKVRLSTDTLLIIKYIREELAAQFDKLGVYVSDRRWQRAA
                     VLLKASAFCNGRKETNHSDVILLKYCLWTTPENRESVEQIVMNTIKSCGFDTGIDLAE
                     LDREKESLDKEINKELYHSQDVYETVEMSGKKYFPVQAVFKNVHNSHANKELKIYVPY
                     SKFRSKEKFYPIDSTGNEIQEIECEFDGQGCCKLELKVRGGHWNEQYKPFKFKPNVLF
                     HKGDKKQSINDRLIWSLSRSVMEINKALLDVLKLVTKKHAEHQQFLQSPFITQDDIDI
                     AVSGILDQIDKLNLRIKDCERLESLCR"
     gene            10704..12164
                     /locus_tag="WIC03_000626"
     CDS             10704..12164
                     /locus_tag="WIC03_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003758253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEZ3883167.1"
                     /translation="MSLNSKLSEQIRWIKASLQIDYADLFKQHGSLNGQIQQRLSDWQ
                     YQTKLALLQKNPFVLHEEKLKKHQQTEIFNQQSVDDYRYFCKLAGVPFEKEFWQQELT
                     QSKNSKPDKQNLPLKLLTEKWQQQLDYAKSQWYLEQLNQLRQEFIDKLKQELDTIRQL
                     AEQLEQFGFEAGLWFDNSIGNLSQQNIEEMKRWLNYLTQDKNAQQIAELLGKMRQIEQ
                     SEKIEQVKQTVYIQNPQIDINSREEIIGLRLGKDLEYVLPSELALMADEETSILFDLK
                     FLESKLMCFELQGTTYCDAPTEIIVEQKSQEDEKLGPMILCVDTSGSMVGLPENIAKA
                     MALFLGAKAKSENRSCFVINFSTGIETFELTSKTGISNLIAFLRQSFHGGTDAAPALR
                     HALKMMEQESYQKADLLMISDFVMNSLPDDLLASIEIQRETGNQFNSLVIGDAFMSKR
                     LKTHFDREWIYNPNVQTIQELVQFKKDIFNKQVVNS"
     gene            complement(12518..12712)
                     /locus_tag="WIC03_000627"
                     /pseudo
     CDS             complement(12518..12712)
                     /locus_tag="WIC03_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3609 a         3366 c         2881 g         2856 t
ORIGIN      
        1 acacgcaaat tcgccccctt caccgccggc agcggcatcc cacaggtcat cgcctcactg
       61 tcgctgccct acggcgcaca gaaaacgcgg ctgatccgcc tcgggcagac gctgctgaag
      121 attccgctga cctttttggg tatgctgttc ggcgcgtcca tcggacgcga aggtccgtcc
      181 gtgcaggtcg gcgcggcagt gatgggcgcg tggggcgcgt ggtgcaagaa acacggcttg
      241 gcattcaaag ggatgcagga aaacgatttg atggcggcgg gcgcggcggg cggtttggca
      301 gccgcgttca acgcgccgct ggcgggcgtg attttcgcca ttgaggaact cgggcgcggc
      361 atcatgttgc gctgggagag gcaaattctt ttgggcgtgc tcgcctccgg tttcatacag
      421 gtcgccattc agggcaacaa cccgtatttt tccggcttca acggcggcgt attggaacat
      481 atctttctgt gggtcgcact gtccggcctg gtttgcggcg cggcgggcgg gctgttcgga
      541 cgtttgctct atcgcggtgc ggcggcattt gcaccacgca agatacgcgg cttcatccgc
      601 aaccgtccgc tgctgctggc ggcactgatg gggctgctgc tcgccctgct cggcacgttc
      661 taccaaggca aaacctacgg caccggctac cacgaagccg cccaagccct gcacggcatc
      721 tatgaagccc ccttcggact cgccgccgcc aaatggctcg ccaccgtatt cagctattgg
      781 gcaggcgttc cgggcggcat tttcactccc tcgctgacca taggcgcggt tttgggcgag
      841 catatcgccg ccatcgccga catatcgcag ggtgcaaaca tcatcgtcct catctgcatg
      901 gcggcatttc tggcgggcgc gacacaatcc ccgattactt ccgccgtcgt cgtcatggaa
      961 atgacgggcg gacaaagcct gctgttttgg atgctaattg cctgcatttt cgcctcgcag
     1021 gtttcgcgcc agttttcgcc gcgtccgttc taccacgcat cgggaatgcg cttccgccag
     1081 cgcgtgcttc aggaaaccac cgcccaaacc ggcaatgcgc ccgcaagacc gcaaacagca
     1141 aacagcaaaa cgggaatgcc gtctgaaaat taaaacgccc ccgatcaaac gccggcagcc
     1201 gccttgattt gaataccgtt ccgccgccgc ttgaaatttc agcaacaatg ccgtctgaac
     1261 gacagaatgc ggttttcaga cggcatttcc acatcccgat attgcctaaa caaaaccgaa
     1321 gcgtttgcta taattctatt ttttaccgca cacgcaccaa tcatgtttcc cgatttctcc
     1381 caaaccctct ccaaagaccg ccacttccta cgttccgcct tcaaaaatcc caacaaatac
     1441 ggcgggttgt ccaaagtcga agaaaaatac cgaaaatcgc acgaaatctt tttgaaacgt
     1501 ttggcagcct tgcccaaacc cgaattcgac aacaccctgc ccgttcacga aaaacttgac
     1561 gaaatcaaaa aaaccgttgc cgaacatcag gtaacgatta tttgcggcga aaccggttcg
     1621 ggcaagacca ctcagttgcc caagatttgt ctggaactcg ggcgcggggc ggcgggtttg
     1681 atcgggcata cccagccgcg ccgtttggcg gcgcgttccg tagcagagcg gattgccgaa
     1741 gagctgaaat ccgaaatcgg cagcgcggtc ggctataaag tgcgcttcac cgaccacacc
     1801 tcgcgcgatg cctgcgtcaa gctgatgacc gacggcatcc tgctggcgga aacccagacc
     1861 gaccgttatc tcgccgccta cgacacgatt atcatcgacg aagcgcacga gcgcagcctg
     1921 aacatcgact tccttttggg ctatttgaaa caactcctgc cgcgccgccc cgatttgaaa
     1981 gtcatcatca cctcggcaac gatagacgca gaacgctttt cccaacactt caacggcgcg
     2041 cccgtgctgg aagtgagcgg acgcacctat cccgtcgaaa tcctctaccg accgctgatc
     2101 ggcaaagacg aagacgacgc ggaaatagaa ttgaccgacg cgattgtcga tgcggcggac
     2161 gaattggcgc gatatggcga aggcgatatt ttggtattcc tgccgggcga acgcgaaatc
     2221 cgcgaagccg ccgaagccct gcgcaaatcc acgctacgcc gcaacaacga aatcctgccc
     2281 ctgttcgcac gcctgtcgca cgccgagcag cacaaaatct ttcacccctc cggagcgaaa
     2341 cgccgcatcg tgctggcaac caacgtcgcc gaaacctcgc ttaccgtgcc gggcatcaaa
     2401 tacgtcatcg acaccggcct cgcgcgcgtc aaacgctatt ccgcacgggc gaaagtggag
     2461 cagcttcata tcgaaaaaat ctcccaagcc gccgcccgcc aacgctccgg ccgctgcgga
     2521 cgcgtctccg caggcgtatg tatccgattg ttttcagaag aaaatttcaa caaccgcccc
     2581 gaatttaccg accccgaaat cgcccgcagc aacctcgccg ccgtcatcct gcgcatggca
     2641 tcgctgaaac tcggcgacgt agcggcattc ccatttttag aaatgcccga ttcgaggtat
     2701 attaatgatg ggtttcaggt gttgctggaa ttaggggcag tggaggccgc ctgaaaacaa
     2761 gtaggatata cacttctacc tgacaaagac caacaaacga taaaactacg gcacatctag
     2821 atgcccaacc tacaacattg cacacggtaa aaacatgaaa cacttactca tcgattttga
     2881 aaacgtccag ccgcaaaact tagacaaatt actaaccgaa aatacccata tttggctatt
     2941 tataggtgta ttacacaaaa tgttacctat tagtctggtg caatccctac tacgtttcgg
     3001 cgaacgtgtc catcttgtcc agttacaaaa aacggggaaa aacgcattgg atttttacct
     3061 gtcctattac ctcggacaaa ttaccgccac agaccccaat gcccaaatcg gcatactctc
     3121 gcgtgatgga ggatacgatg ttctggtcga acatattttg aaaaaccacc aggcgaaggg
     3181 tatcgtgcgc ctagccaata tagatgaagt acaacatcag aaaattacta ccgaaccgcc
     3241 gtcagcattg ctggaaaaca ctcctcagcc tgaaaccacc ctcaaaccac agcaaccatt
     3301 aacttcctat ttccaagcag ccctaactgc actgcgccgc cccgacgctt tccgcccctg
     3361 ccgcctgcat aacctgcgac aaaatctgcg taagcatatt ttgagtgatt tgtttaaaga
     3421 aaaaaccgat gaagaatgcg aaataaccac tgctaacgtt atcaataaac tcaaagcaca
     3481 aaacttcatc agcattgatg aacaggaaac cgtttcctac catctcagtg ataatgattt
     3541 gttacaaaga atccaacgcc atattttaag ccaacgtccc aaaacctacg ctgattttca
     3601 agccgtcgtg caaaaccgag cagatgcact tcacttaaca gtcggtacca acgacattca
     3661 atcctttgcg cgacatttgc gcgaccaaaa cctgatccgc caaaacaatg ggaaaattga
     3721 atatgcaccg tttactgaac ctaaaccaca gccaacgccc aagcagccta aaaaaaccgc
     3781 atgggaacct gatgaaatta tttggaaaaa agtgattgcc gcgttatcgt taaagaaccg
     3841 tcctaataaa accaaaactt tacgcaatac aatccaggca ctcacaaaat ccaatgcaca
     3901 agaaactgac aaactgctac aacatttaca agatacccaa gtcctacgta ttgacggtac
     3961 taaaattgtg tatatcaagt aggatgggca tttatgcctg acctacaaaa ccaaaaacaa
     4021 cctaacaccc ttaatcaact ccacaaaccc ttttcagaca acctcgtttt ttgaaaaaca
     4081 atctgtaaac agataactgc tgaagaatac cgttgccgag ccccaaaacc tgcctgcaac
     4141 ttttattgtg aactgcccat tatgaaaaaa tcccttttcg ttctctttct ctattcatct
     4201 ctacttaccg ccagcgaaat cgcctatcgc tttgtattcg gaattgaaac cttaccggct
     4261 gcaaaaatgg cagaaacgtt tgcgctgaca tttgtgattg ctgcgctgta tctgtttgcg
     4321 cgttataaaa cttcgcggct gctgattgcg ctgtttttcg cgttcagcat tattgccaac
     4381 aatgtacatt atgcggttta tcaaagttgg atgacgggta ttaactattg gctgatgctg
     4441 aaagaggtta ccgaagtcgg cagtgcgggc gcgtcgatgt tggataagtt gtggctgcct
     4501 gcgttgtggg gcgtggcgga agtcgtgctg ttttgtagcc ttgccaagtt ccgccataag
     4561 acgcattttt ctgccgatat attgtttgcc ttcctaatgc tgatgatttt cgtgcgttcg
     4621 ttcgacacga aacaagagca cggtatttcg cccaaaccga catacagccg catcaaagcc
     4681 aattatttca gcttcggtta ttttgtcgga cgcgtgttgc cgtatcagtt gtttgattta
     4741 agcaagattc ctgtgttcaa acagcctgct ccaagcagaa tcgggcaagg cagtattcaa
     4801 aatatcgtcc tgattatggg cgaaagcgaa agcgcggcgc atttgaaatt gtttggctac
     4861 gggcgcgaaa cttcgccgtt tttaacccgg ctttcgcaag ccgattttaa gccgattgtg
     4921 aaacaaagtt attccgcagg ctttatgacg gcagtatccc tgcccagttt ctttaacgtc
     4981 ataccgcatg ccaacggctt ggaacaaatc agcggcggcg ataccaatat gttccgcctc
     5041 gccaaagagc agggctatga aacgtatttt tacagcgctc aggctgaaaa ccaaatggca
     5101 attttgaact taatcggtaa gaaatggata gaccatctga ttcagccgac gcagcttggc
     5161 tacggcaacg gcgacaatat gcccgatgag aagctgctgc cgctgttcga caaaatcaat
     5221 ttgcagcagg gcaggcattt tatcgtgttg caccaacgcg gttcgcacgc cccatacggc
     5281 gcattgttgc agcctcaaga taaagtattc ggcgaagccg atattgtgga taagtacgac
     5341 aacaccatcc acaaaaccga ccaaatgatt caaaccgtat tcgagcagct gcaaaagcag
     5401 cctgacggca actggctgtt tgcctatacc tccgatcatg gccagtatgt tcgccaagat
     5461 atctacaatc aaggcacggt gcagcccgac agctatctcg tgccgctagt gttgtacagc
     5521 ccggataagg ccgtgcaaca ggctgccaac caggcttttg caccttgcga gattgccttc
     5581 catcagcagc tttcaacgtt cctgattcac acgttgggct acgatatgcc ggtttcaggt
     5641 tgtcgcgaag gctcggtaac gggcaacctg attacgggtg atgcaggcag cttgaacatt
     5701 cgcgacggca aggcggaata tgtttatccg caatgagtgg cgtaaaaacc aataaagaca
     5761 aattaagatg atgtcgggga agatgcccga ccgacaagac tatgcaaaat atgaaaaacc
     5821 aagtacgcgg atcaggcatg gatgcacgat ccaatccggc caatgtttca gacggcctgc
     5881 aaaacagttc gagccatatc ggtaccaaca cgcgctaccg cctgaccaag ctcggcgaac
     5941 aaatggcgcg cctgcccatc gacccgaaaa tcgcgcgcat tttgctggcg gcgaagaaac
     6001 acgactgtat ggcggaaata ttggtgattg cgtccgcgct gtcgattcaa gacccgcgcg
     6061 agcggccgtt ggaagcgcgc gatgctgcct ccaaggcaca cgaacgtttt accgacaagc
     6121 agtccgattt ccttgcctat ctgaacattt gggacagctt ccagcgtgag cgcgacaaag
     6181 gtttgtccaa caagcagctg gtgcagtggt gccgccaata tttcttgtcg cacctgcgga
     6241 tgcgcgaatg gcgcgagcta caccaccagc ttgcccaaac tgcgattgaa atgggtttga
     6301 ccaccaaaga agccgctttc agacgttctc ccgaagtcag gcagctcacg tcgtctgaaa
     6361 atgcaggcga ccaagaccta tccgccaaac tcaaacaaaa acaactggat aagaaacaac
     6421 accgcgccca aatccgcgct gccaaagaag cgggctacga acaaatccac cgcgccctgc
     6481 tcaccggcct catcgccaac gtcggcatga aatcacccga cggcaacgac tacaccagca
     6541 cgcgcggcag ccgcttccac cttttccccg cctccgccct gttcaaggcg aagcccaaat
     6601 gggtgatggc ggcagaactc gttgaaacga caaaacttta tgcccgcgac gtagccgcca
     6661 tccagcccga atggatcgag caggaagcgc cgcacctcgt ccgctaccac tatttcgagc
     6721 cgcattggga acaaaaacgc ggcgaagtca tcgccagcga acgggtaacg ctctacggcc
     6781 tgaccgtcct gccgcgccgc cccgtgtcct acggcagaat cgcccccgaa gaagcgcgcg
     6841 aaatctttat ccgcggcgca ttggtggcac aggaatgcga tttgaaagcg gacttttttg
     6901 tccacaacaa aaagctgatt aaagaaatta ccgaactcga acacaaatcg cgcaagcaag
     6961 acgtgctggt cgatgacgaa gccctgtttg cgttttataa cgaacgactg cccgaaatgg
     7021 cttggaaaga cgcgcaaggc agcgtttggg gaagtgaaga ttccgtacgg attattgaat
     7081 ctgacaaagc cgagaggtcg tctgaaaatg agcgcaacga gtttcgtaaa aacaagcgta
     7141 atgggtctcg ccaaaatgaa aatcacggca acaccgtagg ttgggttgaa aacccaacat
     7201 cagccgcaac tgcaaaaact gttgggtttg acaacccaac ctacgctact cagcaaccca
     7261 ccccctcccc cgagagggag ggtcggggag agggcaaaac agttgccgca caaaccaact
     7321 tttccgcaac cgcagcaaac cctctcccgc aggagaggga acagagtgcc tcagcttcaa
     7381 cgttttcaga cgacctgcgt cctgcaaatc tgcagcaaac cgccccctcc cccgtggggg
     7441 agggctgggg agagagcaaa acagttgcca cccaaaccaa cttttccgca acctcaacac
     7501 tttcagacga ccccaaaccc aaaaagcagc ctgcacctca aaaaggccgt ctgaaacccc
     7561 tacccctcgc cgacatccgc accttccaag cctggctcaa aaccgccgag tgcgacaatc
     7621 cgcgcctgct gttcctcagc cgcgacgatc tgatgcaaca cgccgccgca cacattaccg
     7681 aagaacagtt ccccaaattc tggcaaaccg cagacggcaa attcaaactt tcctaccgct
     7741 tcgagccgca ccatccgcta gacggcgtga ccatgaccgt gccgctgacc gtcctcaacc
     7801 gcctgcacgc gccatcgctc gaatggctgg tgcccggtat gttgcgcgaa aaaatccagc
     7861 tgctgattaa agcactgccg aagcaaatcc gccgcatttg tgtgcccgta cccgatttca
     7921 tcaccaaatt cctcgaaagc aatcccgacc gccaagcgac catcattccc cagttggcgc
     7981 actttatcgc caaaagcgcc agcgatatgc ggattctcga gcaaatcgac caagacgcat
     8041 gggcggcgca agaattgccc gaacactgct atctgaatct gcgcattatc gacgacggcg
     8101 gacaagagct tgccggcggc cgcaaactgc acgaattgca acaacagctc ggccaagccg
     8161 ccgccgtcac cttccgcgac aacacccaag aattcgagcg cgacaacgtt accacatggg
     8221 acatcggaac cctgcccgaa tccatcaaat tcgcccgcgg caaacaacag ctcaccggct
     8281 acctcggcct gcaaaaagaa aaagacggcc gcatcgccct gcgcctgttt gacaccaccg
     8341 aagccgccga acaggcacac cgtcaaggcg tcatcgaatt gatgaagctg caattaaaag
     8401 agcaggtaaa agacctgaac aaaggcatcc aaggcttcac ccaagccgcc atgctgctca
     8461 aacacatcaa cgccgacacc ctgcgcgacg acctcaccca agccgtctgc gaccgcgcct
     8521 ttatcggcga agacgagctg ccgcgcaacg aaaaagcctt caaagaacaa atcaaacgcg
     8581 cccgtagccg cctgcccgcc gtcaaagaag ccctcagccg ctacctgcag gaaaccgcag
     8641 ccgcctacgc cgaactcaac ggcaaactcg gcaaacatcc attgacccac ctcctaagac
     8701 tacgcctgca aaccctgctt gcccccggct tcgccacccg caccccgtgg gcacaatggc
     8761 cgcgcctccc catctacctc aaagccatga ccctgcgcct cgaaaaatac agcagcaacc
     8821 ccgcccgcga cgcagcccgc gaagccgata tacaggaatt ggaacaaatg tggcaggaaa
     8881 aaaacgacgg tttggtgaaa caaggtctcc ccatttcaga cggcctcgcc gcgtttaaat
     8941 ggatgattga agaattgagg gtgtcgctgt tcgcgcagga attgaagacg ccttatccgg
     9001 tgtcggtgaa gcggttgatg aaggagtggg agaggttgaa taagtagtga tattatccaa
     9061 tacatttaca ggaaaatttc taacttacat aaaaggtaca actcaatgaa aagcgaaaat
     9121 aattatgaac gtattgttca aaattctaaa aaattccaac actctcagaa gaatgagatg
     9181 cagaaacgca taacagattt gctgcaacaa ctcacaaatg gcattcatga acgcgaccaa
     9241 attatggcgg tttccctact tggtgcgatt gccggacata atactttcct atttggaccg
     9301 ccgggaaccg cgaaaagtct gatttcccgc cgtttggcat gtgcatttga aaatccgcaa
     9361 tactttgaat acttgatgaa ccgtttcagc acacctgaag aagtattcgg acctgtatct
     9421 attaaggcat tgaaagaaga ccgatatctc cgaaaaatcg aaggctattt gccaactgcc
     9481 gattttgcat ttttggatga aatttggaaa tccagtcctg caattttgaa taacctgctg
     9541 accattatca acgagcatat ttttaaaaac ggcgatgaga ggtttaatgt gccacttaaa
     9601 gccctgattg cggcttccaa cgaaattcca cctgaaaatc aaggtttaga ggcactgtat
     9661 gaccgcttta ttatccgtct gcttgtgccg ccgattgaac aagaagagaa cttcaaccgt
     9721 ttgctggata gcaagccaag cagcgacaaa cctgaaatca gtgaagattt acggattagt
     9781 tatcatgagc ttgccaaatg gcgtgaacaa ctccacaaag tcaggctaag tacagataca
     9841 ttactgatta ttaagtatat cagagaagaa ttagcagccc agtttgataa gttaggcgtt
     9901 tatgtatccg accgacgttg gcaacgtgca gccgtattat taaaggcttc tgctttttgc
     9961 aatggacgca aagaaaccaa tcatagcgat gtgatcttgc tgaaatattg tctgtggaca
    10021 acacctgaaa atcgagagtc agtggaacaa attgtgatga ataccattaa atcctgtggg
    10081 tttgatacag gtattgatct ggctgaatta gatagagaaa aagagagttt ggataaggaa
    10141 ataaataagg aactttatca ttcacaagat gtttatgaga cggttgaaat gagtggaaaa
    10201 aaatattttc cagtacaggc tgttttcaaa aacgtacata atagtcatgc taataaagaa
    10261 cttaagattt atgttccata tagcaaattt cgtagtaaag aaaaatttta tccaatagat
    10321 agtacaggaa atgaaattca agaaattgaa tgtgaatttg atgggcaagg ttgctgtaaa
    10381 ttggaattga aagtgcgggg tggtcactgg aatgagcaat ataagccatt caaatttaaa
    10441 cctaacgttt tattccataa gggagataaa aaacaaagca tcaatgatag gttgatttgg
    10501 tctttatcac gttctgttat ggaaatcaat aaagcattat tagatgtatt gaaattagtc
    10561 accaaaaaac atgcagaaca tcagcaattc ctacaaagcc cttttattac ccaagatgat
    10621 attgatattg cggtatcagg aattttagac caaatcgaca aactcaattt acggattaaa
    10681 gactgcgaac gtttggagtc tttatgtcgt taaatagcaa attatccgaa caaataagat
    10741 ggattaaagc ttctctacaa atagattatg cagatttatt taaacagcat ggttcgttaa
    10801 atggacagat tcagcaacgt ttatcagatt ggcaatatca gaccaaactg gctttgttgc
    10861 agaaaaaccc gtttgtatta catgaagaaa agctaaaaaa acaccaacaa acagaaattt
    10921 ttaatcaaca gtctgttgat gattatcggt atttttgcaa attagcaggc gtgccatttg
    10981 aaaaagaatt ctggcagcag gaattaacac aaagcaaaaa ttcaaaaccc gataaacaga
    11041 atttgccttt gaaattgcta acagaaaaat ggcagcagca attagactat gccaaatctc
    11101 aatggtattt agagcagctt aatcaattac gacaggaatt tatagacaag ttaaaacagg
    11161 aactcgacac tatccgacag cttgcagaac agttagagca atttggtttt gaagcagggc
    11221 tgtggtttga taacagtatc ggcaatttaa gccagcaaaa tattgaagaa atgaaacgtt
    11281 ggctgaatta cttgacccaa gataagaatg cccaacagat tgccgaatta ctaggcaaaa
    11341 tgcgccaaat cgaacaatcc gaaaaaatcg aacaggtaaa acagactgtt tacatccaaa
    11401 atccacaaat tgatataaat tcgcgtgaag aaattatcgg attacgttta ggtaaagatt
    11461 tagagtatgt attgccgtct gaattggctt taatggcaga tgaagaaacg tcgattttat
    11521 ttgatttgaa gtttttggaa tccaaattaa tgtgttttga attgcaagga acgacctatt
    11581 gcgatgcccc aactgaaatt atagttgaac aaaaaagtca agaggatgaa aagctcggac
    11641 caatgatttt atgtgtagat accagtggtt caatggttgg actacctgaa aatattgcca
    11701 aagcaatggc tttattttta ggagcaaaag ctaaaagcga aaaccgttct tgttttgtta
    11761 tcaatttttc cactggaata gaaacttttg aattaacaag taaaacaggg atttccaatt
    11821 tgattgcttt tttacgtcaa tcctttcatg gtggtacaga tgctgcccca gctttacgtc
    11881 atgcgctaaa aatgatggag caagaaagct atcaaaaggc agacttatta atgatttccg
    11941 attttgtgat gaacagcttg cctgatgatt tgttggcatc aatcgaaata caacgagaaa
    12001 ccggtaatca gtttaattct ttggttattg gtgatgcttt tatgagtaaa cgcctaaaaa
    12061 cacattttga tcgtgagtgg atatacaatc ctaatgttca gacaattcaa gaattagtac
    12121 agttcaagaa agatattttc aataaacaag ttgtcaactc ataacactat cctactcata
    12181 tgtatttttg gaagatacca ccctatacaa cttggaatcc atacgcagaa tatgtgtgga
    12241 ctttctaaac tactcccctt tccaccctaa aaaaggtcgt ctgaaaccca ttttcagacg
    12301 acctttttcc acttcgcaaa acaagaattc ccacccctct ccccaaacgt cgcccgaacc
    12361 agtaaactcc ccatatttca acaacttctt ggaagcaaac catgtccggt atctacctac
    12421 ctcgcctatt tccgccccac atcgtcgaac gcggcctttt gtattttcag cagggcaagg
    12481 ttcgcgatgt ccgaaaaact tccgccgggc gttatcgggc ctggcgccgt ttgctgccgg
    12541 cggtctgaat aatggtggcg tcaacgacgg cagcggatgc tttctctact tttaggtttt
    12601 tttcggtcag ttggcggtta atcagtttga gcaattcgga cagggtgtcg tcttgcgcca
    12661 gccagttgcg gtaacggcat aaggtgctgt aatcggggat gctcagttcg tc
//
LOCUS       DAWHSK010000033        12075 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.033, whole genome shotgun sequence.
ACCESSION   DAWHSK010000033 DAWHSK010000000
VERSION     DAWHSK010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12075
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.033"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(332..1108)
                     /gene="lpxA"
                     /locus_tag="WIC03_000628"
     CDS             complement(332..1108)
                     /gene="lpxA"
                     /locus_tag="WIC03_000628"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEZ3883168.1"
                     /translation="MTLIHPTAVIDPKAELDSGVKVGAYTVIGPNVQIGANTEIGPHA
                     VINGHTSIGENNRIFQFASLGEIPQDKKYRDEPTKLIIGNGNTIREFTTFNLGTVTGI
                     GETRIGDDNWIMAYCHLAHDCVIGNHTIFANNASLAGHVTIGDYVVLGGYTLVFQFCH
                     IGDYAMTAFAAGVHKDVPPYFMASGYRAEPAGLNSEGMRRNGFTAEQISAVKDVYKTL
                     YHRGIPFEEAKADILRRAETQAELAVFRDFFAQSARGIIR"
     gene            complement(1195..1644)
                     /gene="fabZ"
                     /locus_tag="WIC03_000629"
     CDS             complement(1195..1644)
                     /gene="fabZ"
                     /locus_tag="WIC03_000629"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEZ3883169.1"
                     /translation="MDVQLPIEAKDIQKLIPHRYPFLQLDRITAFEPMKTLTAIKNVT
                     INEPQFQGHFPDLPVMPGVLIIEAMAQACGTLAILSEGGRKENEFFFFAGIDEARFKR
                     QVIPGDQLVFEVELLTSRRGIGKFNAVAKVDGQVAVEAIIMCAKRVV"
     gene            complement(1678..2724)
                     /gene="lpxD"
                     /locus_tag="WIC03_000630"
     CDS             complement(1678..2724)
                     /gene="lpxD"
                     /locus_tag="WIC03_000630"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEZ3883170.1"
                     /translation="MIPATYTLSQITARLGGEWRGEDISVTAVRPLADAQAEHISFLA
                     NPKYKAEVHDSSAGAVIVSAKAADGFEGRNLIVADDPYLYFAKVARLFSPVVKARGGI
                     HPTAVVEPGATVPASCEIGANVYIGANTVLGEGCRILANAVVQHDCKLGDEVVLHPNA
                     VVYYGCTLGRRVEIHSGAVIGADGFGLAFAGDSWFKIPQTGAVTLGNDVEIGSNTNID
                     RGAMSDTTVGNGTKIDNQVQIGHNCKIGSHTVIAAKTGISGSVTIGSYCIIGGGVGTV
                     GHIEIADKTTIGGGTSVTHSITESGKHLAGIFPMSTHKEWARNAVYIHRLSEMNKRLK
                     TLEQQLSDAGQDSK"
     gene            complement(2757..3257)
                     /locus_tag="WIC03_000631"
     CDS             complement(2757..3257)
                     /locus_tag="WIC03_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEZ3883171.1"
                     /translation="MTRLTRAFAAALIGLCCTAGAHADTFQKIGFINTERIYLESKQA
                     RKIQKTLDSEFSARQDELQKLQREGLDLERQLAEGKLRNAKKAQAEEKWRGLVAAFRK
                     KQAQFEEDYNLRRNEEFASLQQNANRVIVKIAKQEGYDVILQNVIYVNTQYDVTDSVI
                     KEMNAR"
     gene            complement(3323..5716)
                     /gene="bamA"
                     /locus_tag="WIC03_000632"
     CDS             complement(3323..5716)
                     /gene="bamA"
                     /locus_tag="WIC03_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEZ3883172.1"
                     /translation="MKLKQIASALMVLGISPLALADFTIQDIRVEGLQRTEPSTVFNY
                     LPVKVGDTYNDTHGSAIIKSLYATGFFDDVRVETADGQLLLTVIERPTIGSLNITGAK
                     MLQNDAIKKNLESFGLAQSQYFNQATLNQAVAGLKEEYLGRGKLNIQITPKVTKLARN
                     RVDIDITIDEGKSAKITDIEFEGNQVYSDRKLMRQMSLTEGGIWTWLTRSNQFNEQKF
                     AQDMEKVTDFYQNNGYFDFRILDTDIQTNEDKTKQTIKITVHEGGRFRWGKVSIEGDT
                     NEVPKAELEKLLTMKPGKWYERQQMTAVLGEIQNRMGSAGYAYSEISVQPLPNAETKT
                     VDFVLHIEPGRKIYVNEIHITGNNKTRDEVVRRELRQMESAPYDTSKLQRSKERVELL
                     GYFDNVQFDAVPLAGTPDKVDLNMSLTERSTGSLDLSAGWVQDTGLVMSAGVSQDNLF
                     GTGKSAALRASRSKTTLNGSLSFTDPYFTADGVSLGYDVYGKAFDPRKASTSIKQYKT
                     TTAGAGIRMSVPVTEYDRVNFGLVAEHLTVNTYNKAPKHYADFIKKYGKTDGTDGSFK
                     GWLYKGTVGWGRNKTDSALWPTRGYLTGVNAEIALPGSKLQYYSATHNQTWFFPLSKT
                     FTLMFGGEVGIAGGYGKTKEIPFFENFYGGGLGSVRGYESGTLGPKVYDEYGEKISYG
                     GNKKANVSAELLFPMPGAKDARTVRLSLFADAGSVWDGKTYDDNSSSATGGRVQNIYG
                     AGNTHKSTFTNELRYSAGGAVTWLSPLGPMKFSYAYPLKKKPEDEIQRFQFQLGTTF"
     gene            complement(5773..7113)
                     /gene="rseP"
                     /locus_tag="WIC03_000633"
     CDS             complement(5773..7113)
                     /gene="rseP"
                     /locus_tag="WIC03_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEZ3883173.1"
                     /translation="MHTLLAFIFAILILVSLHEFGHYIVARLCGVKVVRFSVGFGKPF
                     FTRKRGDTEWCLAPIPLGGYVKMVDTREGEVSEADLPYAFDKQHPAKRIAIVAAGPLT
                     NLALAVLLYGLSFSFGVTELRPYVGTVEPDTIAARAGFQSGDKIQSVNGTPVADWGSA
                     QTEIVLNLEAGKVAVGVQTASGAQTVRTIDAAGTPEAGKIAKNQGYIGLMPFKITTVA
                     GGVEKGSPAEKAGLKPGDRLTAADGKPITSWQEWANLTRQSPGKKITLNYERAGQTHT
                     ADIRPDTVEQPDHTLIGRVGLRPQPDRAWDAQIRRSYRPSVVRAFGMGWEKTVSHSWT
                     TLKFFGKLISGNASVSHISGPLTIADIAGQSAELGLQSYLEFLALVSISLGVLNLLPV
                     PVLDGGHLVFYTAEWIRGKPLGERVQNIGLRFGLALMMLMMAVAFFNDVTRLLG"
     gene            complement(7148..8383)
                     /locus_tag="WIC03_000634"
     CDS             complement(7148..8383)
                     /locus_tag="WIC03_000634"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEZ3883174.1"
                     /translation="MPSENASDGIRYKVIPIMTPQVLTILGSTGSIGESTLDVVSRHP
                     EKFRVFALAGHKQVEKLAVQCRTFRPEYAVVADAEHAARLEALLKGDGTATQVLHGAQ
                     ALVDVASADEVSGVMCAIVGAVGLPSALAAAQKGKTIYLANKETLVVSGALFMETARA
                     NGAAVLPVDSEHNAIFQVLPRDYAGRLNEHGIASIILTASGGPFLTADLNTFDSITPA
                     QAVKHPNWSMGRKISVDSATMMNKGLELIEAHWLFNCPPDKLEVVIHPQSVIHSMVRY
                     RDGSVLAQLGNPDMRTPIAYCLGLPERIDSGVGDLDFDALSALTFQKPDFDRFPCLKL
                     AYEAMNAGGAAPCVLNAANEAAVAAFLDGQIKFTDIAKTVAHCLSQDFSDGIGDIGGL
                     LAQDARTRAQARAFIGTLR"
     gene            complement(8388..9185)
                     /locus_tag="WIC03_000635"
     CDS             complement(8388..9185)
                     /locus_tag="WIC03_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEZ3883175.1"
                     /translation="MLKQRVITAMWLLPLMLGMLFYAPQWLWAAFCGLIALIALWEYA
                     RMSGLCKIKTNHYLAATLVFGVVAYAGGWMLPNLVWYVVLAFWLAIMPLWLRFKWRLN
                     GGWQVYAVGWLLVMPFWFALVSLRPHPDDALPLLAVIGLVWVADVCAYFSGKAFGKHK
                     IAPAISPGKSWEGAIGGAVCVAVYMTAVRSAGWLAFDTGWFDTVLIGLVLTVVSVCGD
                     LLESWLKRAAGIKDSSNLLPGHGGVFDRTDSLIAVISVYAAMMSVLN"
     gene            complement(9188..9934)
                     /locus_tag="WIC03_000636"
     CDS             complement(9188..9934)
                     /locus_tag="WIC03_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEZ3883176.1"
                     /translation="MKSSTQAVLEHTAIPKHIAVIMDGNGRWAKKRFLPRIMGHKRGL
                     DALENMVKHCAKLGVQYLTVFAFSTENWRRPEDEVSFLMGLFLQALQKQVRRLHENNM
                     RLKILGSRERFNRQILQGIEEAEALTANNTGLTLSIAADYGGRWDILQAANKLIAEGV
                     SEITEDTLAKHLMLGDAPEPDLFIRTGGETRISNFLLWQMAYAELYFTDILWPDFDGK
                     ALDDAVASFQKRERRFGRTSEQLPIEQQRN"
     gene            complement(9990..10547)
                     /gene="frr"
                     /locus_tag="WIC03_000637"
     CDS             complement(9990..10547)
                     /gene="frr"
                     /locus_tag="WIC03_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEZ3883177.1"
                     /translation="MINDIQKTAEGKMQRSVEVLKENLAKVRTGRAHTGLLDQVEVEY
                     WGSMVPVSQVANVTLLDARTIGVKPFEGNMAVKVEKAIRDSNLGLNPAAVGDLIRVPM
                     PMLTEERRKDLIKVVRGEAEEGRVSIRNVRRDANDHIKKLLKDKEISEDEARRGEEAV
                     QKLTDKYITEADKLLTAKEEDLMAI"
     gene            complement(10727..11854)
                     /locus_tag="WIC03_000638"
     CDS             complement(10727..11854)
                     /locus_tag="WIC03_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEZ3883178.1"
                     /translation="MNTSQRNRLVSRWLNSYERYRYRRLIHAVRLGGAVLFATASARL
                     LHLQHGEWIGMTVFVVLGMLQFQGAIYSKAVERMLGTVIGLGAGLGVLWLNQHYFHGN
                     LLFYLTVGTASALAGWAAVGKNGYVPMLAGLTMCMLIGDNGSEWFDSGLMRAMNVLIG
                     AAIAIAAAKLLPLKSTLMWRFMLADNLTDCSKMIAEISNGRRMTRERLEENMAKMRQI
                     NARMVKSRSHLAATSGESRISPAMMEAMQHAHRKIVNTTELLLTTAAKLQSPKLNGSE
                     IRLLDRHFTLLQTDLQQTVALINGRHARRIRIDTAINPELEALAEHLHYQWQGFLWLS
                     TNMRQEISALVILLQRTRRKWLDAHERQHLRQSLLETREHG"
     gene            complement(11930..12005)
                     /locus_tag="WIC03_000639"
     tRNA            complement(11930..12005)
                     /locus_tag="WIC03_000639"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11970..11972),aa:Thr,seq:cgt)
BASE COUNT         2268 a         3081 c         3768 g         2958 t
ORIGIN      
        1 tttaaaacac cacttttgta gtgtccagta atttcagcac agaatattca ataagacaat
       61 atgttctttt gaaaaatact tttggttttt tcgccgaaag caggtcggtt caagctgcgg
      121 aaattgtttg caattcttta aaagcagcgg cggaggtcac aatgaaatgt ccgaatgggg
      181 atgtggtcgg cggcagaaat catcaatgct gccgactgcc atacttctga aatctacaaa
      241 atgatgcatc gatcaaacaa tataccgctt taaaaaaacc gatgccgtct gaaacgcttt
      301 cggggtttca gacggcatca aaagggtacg gtcagcggat gatgccgcgc gccgattgtg
      361 cgaaaaagtc tcggaatacg gcaagctcgg cttgggtttc ggcgcggcgg aggatgtctg
      421 ccttggcctc ttcaaacgga atgccgcgat ggtagagggt tttgtacacg tctttgacgg
      481 cggaaatctg ttctgcggta aaaccgttgc ggcgcatgcc ttcgctgttg agccccgccg
      541 gttcggcgcg gtagcccgat gccataaagt agggcggtac gtctttgtgt acgcctgcgg
      601 cgaaggcggt catggcgtag tcgccgatgt ggcagaattg gaaaaccagc gtgtagccgc
      661 ccaaaacgac gtagtcgccg atggtaacgt gtccggcaag cgaggcgttg ttggcgaaga
      721 tggtgtggtt gccgatgacg cagtcgtgcg cgaggtggca gtacgccata atccagttgt
      781 cgtcgccgat acgggtttcg ccgatgccgg ttaccgtgcc taaattaaag gtggtgaatt
      841 cgcggatggt gttgccgttg ccgataatca gcttggtcgg ctcgtcgcgg tattttttgt
      901 cctgcgggat ttcgccgagg ctggcaaatt ggaaaatgcg gttgttttcg ccgatgctgg
      961 tgtggccgtt gatgacggcg tgcggaccga tttcggtatt cgcgccgatt tggacgttgg
     1021 ggccgataac ggtgtacgcg ccgactttga cgccggagtc gagttcggct ttggggtcga
     1081 tgacggcggt cgggtggatg agggtcatgt ttttcctttc ctgtcgtgtt gccgcgaaga
     1141 tgcgcgacgg caacaggttg tctgaaaact ttcagacgac ctttttctga acactcaaac
     1201 tacgcgtttg gcacacatga tgatggcttc gacggcaact tgcccgtcca ctttggcgac
     1261 ggcgttgaat ttgccgatgc cgcgccggct ggtcagcagc tcgacttcaa agacgagttg
     1321 gtcgccgggg atgacttggc gtttgaaacg ggcttcgtct atgccggcga agaagaagaa
     1381 ttcgttttct ttgcgcccgc cttcgctcaa aatcgccaac gtgccgcacg cctgcgccat
     1441 cgcttcgatg atgagtacgc cgggcatcac gggcaggtcg ggaaaatggc cttggaactg
     1501 gggttcgttt atggtgacgt ttttaatcgc ggtcagggtt ttcatcggct cgaaggcggt
     1561 gatgcggtcg agctggagaa acggatagcg gtgggggatg agtttttgga tgtctttggc
     1621 ttcgatgggg agttgtacgt ccatgtctgt cgtattcctt gaataaagtc ggtttggtta
     1681 tttgctgtct tgaccggcat ctgaaagctg ctgctccagt gttttgagcc gtttgttcat
     1741 ttcgcttaag cggtggatgt aaacagcgtt gcgcgcccat tctttatggg tggacatcgg
     1801 gaagatgccg gcgaggtgtt tgccgctttc ggtaatgctg tgggtgacgg acgtgccgcc
     1861 gccgatggtg gttttgtcgg cgatttcgat gtgtccgacc gtaccgacgc cgccgccgat
     1921 gatgcagtag ctgcctatgg ttacgctgcc tgagatgccg gttttggcgg cgatgacggt
     1981 gtgcgaaccg attttgcagt tgtgtccgat ttggacttgg ttgtcgattt tggtgccgtt
     2041 gccgacggtg gtgtcgctca tcgcgccgcg gtcgatgttg gtgttcgagc cgatttctac
     2101 gtcgttgccc agcgttaccg cgccggtttg cgggatttta aaccatgaat cgccggcaaa
     2161 agcgagtccg aaaccgtccg cgccgatgac cgcgccgctg tggatttcga cgcgtctgcc
     2221 cagtgtgcag ccgtaataaa cgacggcgtt gggatgcagg acgacttcgt cgcccagttt
     2281 gcaatcgtgt tggacgacgg cgtttgccaa gatgcggcag ccttcgccga gcacggtgtt
     2341 tgcgccgatg tagacgttcg cgccgatttc gcagctggcg ggaacggtcg cgcccggttc
     2401 gacgacggcg gtcggatgga tgccgccgcg cgctttgacg acgggtgaaa acaggcgggc
     2461 gactttggcg aaatagagat aggggtcgtc ggcgacaatc aggttgcgcc cttcaaatcc
     2521 gtctgccgct ttggcggaaa cgatgaccgc gcccgcgctg ctgtcgtgga cttcggcttt
     2581 gtatttcgga ttggcaagga agctgatgtg ttccgcctgc gcgtctgcga gcgggcgcac
     2641 ggcggtaacg gaaatgtcct cgccgcgcca ttcgccgccg agccgcgcgg tgatttggga
     2701 cagggtgtag gtggccggaa tcatggtttt cctgttcggt atgccgtctg aaagggtcag
     2761 cgggcgttca tttctttaat gacgctgtcg gtaacgtcgt attgggtgtt gacgtaaatc
     2821 acgttctgca aaatgacatc gtaaccttcc tgtttggcga ttttgacgat gacgcggttg
     2881 gcgttttgct ggagggaggc aaactcttcg ttgcggcgga ggttgtagtc ttcttcaaac
     2941 tgcgcctgtt ttttgcggaa cgctgcgacc agcccgcgcc atttttcttc ggcttgcgcc
     3001 ttttttgcgt ttctgagttt gccttcggca agctgccttt ccaaatccag accttcgcgt
     3061 tgcagttttt gcaattcgtc ctgacgggcg gaaaattcgc tgtccagcgt tttttgaatc
     3121 ttgcgcgcct gcttggattc gaggtagatg cgctcggtgt tgataaagcc gattttttgg
     3181 aaggtgtcgg cgtgcgcgcc tgcggtgcag cacaaaccga tcagagccgc ggcaaacgcg
     3241 cgggtcaaac gggtcatggt aaaactcctt cgaatgttgc cgcgaaatgc cgtctgaagg
     3301 gcttcagacg gcatttgcgg gattagaacg tcgtgccgag ttggaattgg aagcgttgga
     3361 tttcgtcttc cggttttttc ttcagcgggt aggcgtagct gaatttcatc gggcctaaag
     3421 gcgagagcca ggtaaccgcg ccgccggcgg aatagcgcaa ttcgttggta aaggtggatt
     3481 tatgggtatt gccggcgccg taaatgtttt gaaccctgcc gccggtcgcg gaactgctgt
     3541 tgtcgtcgta ggttttgccg tcccacacgc tgcctgcgtc ggcaaacagg ctcaggcgga
     3601 cggtgcgcgc gtctttcgcg ccgggcatcg ggaagagcag ctcggcggag acgttggctt
     3661 ttttgttgcc gccgtagctg attttttcgc cgtattcgtc atacactttc ggaccgagcg
     3721 tgccgctttc gtatccgcgc accgaaccca ggccgccgcc gtagaagttt tcaaagaagg
     3781 ggatttcttt ggttttgccg tagccgcccg caatgccgac ttcgccgccg aacatcagcg
     3841 tgaaggtttt gcttaagggg aagaaccagg tttggttgtg ggtggcggag tagtattgca
     3901 gtttgctgcc gggcagggcg atttcagcgt tcacgcccgt caggtagccg cgcgtcggcc
     3961 ataacgcgct gtcggttttg ttgcgccccc agccgacggt acctttgtac agccagcctt
     4021 tgaagctgcc gtctgtgccg tcggttttgc cgtatttctt gataaagtcg gcatagtgtt
     4081 tgggcgcttt gttgtaggtg ttgacggtca ggtgttctgc caccaaaccg aaattcacgc
     4141 ggtcgtattc ggtaacaggc acgctcatgc ggatgcctgc gcctgccgtg gtggttttat
     4201 attgtttgat gctggtcgat gctttgcgcg ggtcgaaggc ttttccgtaa acatcgtagc
     4261 ctaggctgac cccgtctgcc gtgaagtacg ggtcggtaaa cgacagcgag ccgttgagcg
     4321 tggttttgct tcgtgaggcg cgcagggcgg ccgacttgcc cgtaccgaac aggttgtctt
     4381 gggaaacgcc tgcggacatg accaggccgg tatcctgtac ccagcccgcg ctcaagtcga
     4441 gcgagccggt ggaacgttcg gtcaggctca tgttcaaatc gactttgtcg ggtgtgccgg
     4501 caagcgggac ggcatcaaac tgtacgttgt cgaagtagcc caaaagctcg acgcgctctt
     4561 tggagcgttg cagcttggag gtgtcgtaag gcgcggattc catttggcgc aattcgcggc
     4621 gcacgacttc gtcgcgggtt ttgttgttgc cggtgatgtg gatttcgttg acgtagattt
     4681 tccggcccgg ttcgatgtgc aggacgaaat cgacggtttt ggtttcggcg tttggcagcg
     4741 gctgtacgct gatttcgctg tatgcgtagc ctgccgagcc catgcggttc tgaatctcac
     4801 ccaaaacggc ggtcatctgc tggcgttcgt accatttgcc gggcttcatg gtcagcagtt
     4861 tttccagttc ggctttgggg acttcgttgg tgtcgccttc gatggagact ttgccccaac
     4921 ggaaacgtcc gccttcgtgg acggtgattt tgatggtctg cttggttttg tcttcgttgg
     4981 tttggatgtc ggtatcgagg atgcggaaat cgaagtagcc gttgttctgg tagaagtcgg
     5041 ttactttttc catgtcttgg gcaaatttct gctcgttgaa ttggttgctt cgtgtcagcc
     5101 atgtccaaat gccgccttcg gtcagcgaca tctgccgcat cagtttgcgg tcggaataga
     5161 cttggttgcc ttcaaattcg atgtcggtga ttttggcgga tttgccctcg tcaatcgtga
     5221 tgtcgatgtc gacgcggttg cgggcgagtt tggttacttt gggcgtgatt tggatattga
     5281 gtttgccgcg cccgaggtat tcttctttca ggccggcgac tgcctgattg agtgtcgcct
     5341 gattaaagta ttgcgactgc gccagcccga acgattcgag gtttttctta atggcgtcgt
     5401 tttgcagcat ttttgcgccg gtgatgttga gcgagccgat ggtggggcgt tcgataacgg
     5461 tcagcaggag ctgcccgtcc gcagtttcga cgcgtacgtc gtcaaagaaa ccggtggcgt
     5521 acaggctttt gatgatggca ctgccgtgtg tgtcgttgta ggtgtcgccg actttgacgg
     5581 gcaggtagtt gaatacggta ctcggctcgg tacgctgcaa gccttcgacg cggatgtctt
     5641 ggatggtgaa gtcggcaagt gccaaaggcg atatgcccaa gaccatcagt gcggaagcaa
     5701 tctgtttcag tttcattgtc agttccttgt ggtgcggaat gcggtttcag acggcattcc
     5761 gaaacgtaaa atctaaccga gcagccgggt aacgtcgttg aagaaggcga ccgccatcat
     5821 cagcatcatg agggcaagcc cgaagcgcaa accgatgttt tggacgcgtt cgcccaaagg
     5881 tttgccgcgt atccattcgg cagtataaaa cacgaggtgg ccgccgtcca aaacggggac
     5941 gggcagtagg ttcagcacgc cgaggctgat gctgaccagt gccaaaaatt ccaaataact
     6001 ttgcaagccg agttcggcgg actgtccggc aatgtcggca atggtcagcg gcccggaaat
     6061 atggctgacg gaggcgttgc cgctgattag tttgccgaaa aatttgaggg ttgtccacga
     6121 gtgggaaacg gttttttccc agcccatgcc gaatgcgcgg acaacagacg gacggtagct
     6181 gcggcggatt tgcgcgtccc acgccctgtc cggctgcgga cggaggccga cgcgcccgat
     6241 cagggtgtgg tcgggctgtt cgacagtatc ggggcggatg tcggcggtat gggtttgtcc
     6301 ggcgcgttcg tagttcaggg tgattttttt gccggggctt tggcgggtca ggtttgccca
     6361 ttcttgccat gaggtaatgg gtttgccgtc ggcggcagtc agcctgtcgc ccggtttcag
     6421 gcctgctttt tcggcggggc tgcctttttc cacgccaccg gcaacggttg tgattttaaa
     6481 gggcatcagt ccgatgtagc cttggttttt tgcgatttta ccggcttccg gcgtgcctgc
     6541 ggcatcgatg gtgcggacgg tttgcgcgcc tgatgccgtc tgaacgccga cggcgacttt
     6601 gccggcttcg aggttgagga cgatttcggt ttgcgcgctg ccccaatctg caacgggtgt
     6661 gccgttgacg gattgtattt tgtcgccgct ttggaagccg gcgcgggcgg caatggtgtc
     6721 gggttcgact gtgccgacat aggggcgcag ttcggttacg ccgaaggaaa agctcagtcc
     6781 gtacagcaaa accgccagtg cgagattggt cagcgggccg gcggcgacga tggcgatgcg
     6841 tttggcgggg tgttgtttgt caaaagcgta gggtaaatcg gcttctgata cttcgccttc
     6901 gcgcgtatcg accattttga cgtaaccgcc caacggaatc ggggcgaggc accattcggt
     6961 gtcgccgcgc tttcgggtga aaaacggttt gccgaagccg acggaaaagc gtacgacttt
     7021 gacgccgcac aatctggcaa cgatgtagtg tccgaactcg tgcaggctga ccaaaatcag
     7081 gatggcgaag ataaaagcta gaagggtgtg caaatggttt tcctttgata acggtgttca
     7141 gacggcatca gcgcagtgtg ccgataaatg cccgcgcttg tgcgcgtgtc cgggcatctt
     7201 gcgccaagag cccccctatg tcgcctatgc cgtctgaaaa gtcttgtgaa agacaatggg
     7261 cgacggtttt ggcaatgtcg gtaaacttaa tctgtccgtc caaaaaggcg gcgacggcgg
     7321 cttcgttggc ggcgttcaat acgcagggcg cggctccgcc tgcgttcatg gcttcatagg
     7381 cgagcttcag gcaggggaag cggtcaaagt cgggcttttg gaaggtcagc gcggacaatg
     7441 cgtcgaaatc caggtcgccg acacccgaat cgatgcgctc gggcaaaccc aaacaataag
     7501 caatgggcgt acgcatatcg ggattgccca gttgcgccaa cacggagccg tcgcggtagc
     7561 gcaccatgct gtgtatcacg gattgcggat ggatgacgac ttcgagcttg tcaggaggac
     7621 agttgaacag ccaatgcgct tcaatcagct ccaaaccttt gttcatcatg gtggcggaat
     7681 cgacggagat tttgcgcccc atgctccaat tggggtgttt gaccgcttgg gcgggcgtaa
     7741 tgctgtcgaa cgtgtttaaa tcggcggtca gaaacgggcc gccggaagcg gtcaggataa
     7801 tcgaagcgat gccgtgttcg ttcagacggc cggcgtaatc gcgcggcaaa acttggaaaa
     7861 tggcgttgtg ttcgctgtcg acgggcagca ctgccgcgcc gtttgcacgg gcggtttcca
     7921 taaacaacgc gccggaaacc accagcgtct ctttgttcgc cagataaatg gttttgcctt
     7981 tttgcgccgc tgcgagcgcg gaaggcagcc ccaccgcccc gacgatggcg cacatgacac
     8041 cgctgacttc gtcggcagag gcaacgtcaa ccaatgcctg cgcgccgtgt aaaacctgcg
     8101 tcgccgtgcc gtcgcctttc aacaaagctt caagccgggc ggcgtgttcg gcatcggcaa
     8161 cgacggcata ttcggggcgg aacgtccggc attgcaccgc cagtttctcg acctgcttat
     8221 gccctgccag cgcgaatacg cggaattttt cggggtggcg ggagacaacg tccagcgtgc
     8281 tttcgcctat gctgccggta ctgcctaata tggtcaggac ttgtggtgtc ataatgggga
     8341 taactttata ccggatgccg tctgaagcgt tttcagacgg catagaatca atttaaaacc
     8401 gacatcatcg ccgcatagac gctgataacg gcaatcaggc tgtcggtacg gtcgaacacg
     8461 ccgccgtgtc cgggcagcag gttgctgctg tctttgatgc ctgccgcgcg cttgagccag
     8521 ctttccaaaa ggtcgccgca tacgctgaca acggtcaaca ccaaaccgat taacacggta
     8581 tcgaaccagc ctgtatcgaa tgccagccag ccggcacttc gtacggcggt catgtacacg
     8641 gccacgcaaa ccgcgccgcc gattgcgcct tcccagcttt tgccagggct gattgccggt
     8701 gcgattttgt gtttaccgaa cgccttgccg ctgaaatacg cgcaaacgtc ggcaacccag
     8761 accagaccta tcacggcgag cagcggcagg gcatcatcgg gatgcgggcg cagggatacg
     8821 agcgcgaacc aaaacggcat gaccagaagc caaccgacgg cataaacctg ccaaccgccg
     8881 ttgagcctcc atttgaatct caaccataaa ggcatgatgg cgagccaaaa tgccaaaaca
     8941 acataccaaa ccaaattagg cagcatccag ccgcccgcat aggcaaccac gccgaaaacc
     9001 aaggttgcgg cgaggtaatg gttggtttta attttgcaca aaccgctcat acgggcatat
     9061 tcccacaagg caatcagggc aatcagtccg caaaatgcag cccacaacca ttgcggcgcg
     9121 taaaacagca tgcccagcat cagcggcagc agccacatag cagttatcac ccgttgtttc
     9181 agcatattca gttcctttgc tgttcgatag gcagttgctc ggaggtgcgt ccgaaccgcc
     9241 gttcgcgttt ttggaacgaa gcgacggcat cgtccaaagc cttgccgtca aaatcgggcc
     9301 acaaaatatc ggtgaaatac agttctgcat atgccatctg ccagagcagg aaattgctga
     9361 tgcgcgtttc gccgccggtg cggatgaaca aatccggttc cggtgcatcg cccagcatca
     9421 agtgtttcgc cagcgtgtct tccgtaatct cggatacgcc ttcggcaatc agtttgtttg
     9481 ccgcctgcaa aatatcccaa cgtccgccgt aatcggcggc aatgctcagg gtcaggccgg
     9541 tattgtttgc cgtcaaggct tccgcctctt cgatgccttg cagaatctgc cgattaaagc
     9601 gttcgcggct gcccagtatt tttaggcgca tattgttttc gtgcagacgg cgtacctgtt
     9661 tttgcaaagc ctgtaaaaac agccccatca ggaacgaaac ttcgtcttcg gggcggcgcc
     9721 agttttcggt tgaaaaggca aacacggtca gatattgcac acccagtttg gcgcaatgct
     9781 tcaccatatt ttccaatgcg tccaaaccgc gtttgtgtcc cattatgcgc gggaggaaac
     9841 gttttttcgc ccaacggccg ttgccgtcca taatcacggc gatatgcttg ggaatggcgg
     9901 tgtgttccaa aacggcctgc gtgctgcttt tcatgtctgc ctttcgcggt tcggcattca
     9961 aatgccgtct gaacgccgaa ccgtgcaggt taaattgcca tcaaatcttc ttctttggca
    10021 gtcaggagtt tgtcggcttc ggtaatgtat ttgtcggtca gtttttgaac cgcttcttcg
    10081 ccgcgacgtg cctcgtcttc ggaaatttct ttgtctttga ggagtttttt gatgtggtcg
    10141 ttggcatcgc ggcgcacgtt gcggatggag acgcggcctt cttccgcttc gccgcgtacg
    10201 actttaatca ggtctttgcg gcgttcttcg gtcagcatgg gcatcggcac gcggatcagg
    10261 tcgccgacag ctgccgggtt cagccccaag tttgaatcgc ggatggcttt ctcgactttg
    10321 accgccatat tgccctcaaa cggtttcacg ccgatggtgc gcgcgtccag aagcgttacg
    10381 ttggcaactt ggctgacggg gaccatgctg ccccagtatt cgacttccac ttggtcgagc
    10441 aggccggtat gcgcgcggcc ggtacgcact ttcgccagat tttctttcag tacttcgacc
    10501 gaacgctgca ttttgccttc ggctgttttt tgaatatcgt tgatcatatt gttctttcgg
    10561 tgggataagg tgggcgggag accgtctgaa cgcgtttcaa gccgttcaga cggcataaag
    10621 accgttaacc gcgaatagta ccgttattcg ggcataacga caaggtaggc ggattgggga
    10681 tgccgtctga agcgacaggc gtttcagacg gcatcgtgtc cgaccgtcag ccgtgttccc
    10741 gtgtttcaag caggctttgg cgcaggtgtt ggcgttcgtg ggcatccagc catttgcggc
    10801 gggtgcgttg cagcaggatg acgagggcgg aaatttcctg acgcatattg gtgctgagcc
    10861 agaggaagcc ctgccattgg tagtggaggt gttcggcgag ggcttccagt tcggggttga
    10921 tagcggtgtc gatgcggatg cggcgggcgt gtctgccgtt gataagggcg acggtttgtt
    10981 gcaggtcggt ttggagcagt gtgaagtggc ggtcgagcag ccggatttcg ctgccgttga
    11041 gtttgggaga ttgcagcttg gcggcagtgg tcaggagcag ctcggtggtg ttgacaattt
    11101 tacggtgggc gtgctgcatg gcttccatca tggcggggct gatgcggctt tcgcccgatg
    11161 tggcggcgag gtggctgcgg cttttgacca tgcgtgcgtt gatttggcgc attttcgcca
    11221 tgttctcttc gaggcgttcg cgggtcatgc gcctgccgtt gctgatttcg gcaatcattt
    11281 tgctgcagtc ggtcaggttg tcggcaagca tgaaacgcca catcagtgtg gatttcagcg
    11341 gcagcagttt ggcggcggcg atggcgatgg ccgcgccgat gaggacgttc atcgcgcgca
    11401 tcaggccgct gtcgaaccat tcgctgccgt tgtcgccgat gagcatgcac atcgtcagcc
    11461 ccgccagcat agggacgtag ccgtttttgc cgaccgccgc ccagccggcc agtgcgcttg
    11521 ccgtgccgac ggtgaggtag aagaggaggt tgccgtggaa ataatgctgg ttcagccata
    11581 aaacgcccaa acccgcgccc agcccgatga ccgtgccgag catacgttcc accgccttgg
    11641 agtaaatcgc cccttgaaac tggagcatgc cgaggacgac gaagacggtc atccctatcc
    11701 actcgccgtg ttggaggtgg agcagccggg cggaggcggt ggcgaacagg acggccccgc
    11761 cgagccggac ggcgtggatg aggcggcggt agcggtagcg ttcgtaggag ttgagccagc
    11821 ggctgacgag gcggttgcgt tgcgaggtgt tcatatcggt tgtgccgtct gaagcggaaa
    11881 tgtgaaaaag cacaggcttc ccgaggaagg gagggtctgt gcttggtatt ggtgccggag
    11941 aagggaatcg aacccccgac cttcgcgtta cgaatgcgct gctctaccga ctgagctaca
    12001 ccggcgtttt ttcgtcatga tatatatgaa cggttgtttg tgcaactttt cgggcgggcg
    12061 gcaaggcagt gcgcg
//
LOCUS       DAWHSK010000034        11811 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.034, whole genome shotgun sequence.
ACCESSION   DAWHSK010000034 DAWHSK010000000
VERSION     DAWHSK010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11811
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.034"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(53..538)
                     /gene="purE"
                     /locus_tag="WIC03_000640"
     CDS             complement(53..538)
                     /gene="purE"
                     /locus_tag="WIC03_000640"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEZ3883179.1"
                     /translation="MIQIGIIMGSNSDWPVMRQAAQFLEEFGVEYEARVVSAHRTPDL
                     MFQYAETARERGIKAIIAGAGGAAHLPGMVAAKTTVPVLGVPVPSKYLRGEDSLLSIV
                     QMPKGVPVATFAIGEAGAANAALFAVSMLANETPELAQKLADFRAKQEQAVLSMELEQ
                     I"
     gene            complement(586..1302)
                     /locus_tag="WIC03_000641"
     CDS             complement(586..1302)
                     /locus_tag="WIC03_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system YbgF family protein"
                     /protein_id="HEZ3883180.1"
                     /translation="MKTKLPLFIIWLSVSAACSSPVSRNIQDMRPEPQAEAGSSDAIP
                     YPVPTLQDRLDYLEGKIVRLSNEVETLNGKVKALEHAKTHPSGRTYVQKLDDRKLKEH
                     YLNTEGGSASAHTVETAQNLYNQALKHYKSGRFSAAAALLKGADGGDGGSIAQRSMYL
                     LLQSRARMGNCESVIEIGGRYANRFKDSPTAPEAIFKIGECQYRLQQKDIARATWRSL
                     IQAYPGSPAAKRAASAIRKR"
     gene            complement(1302..1970)
                     /locus_tag="WIC03_000642"
     CDS             complement(1302..1970)
                     /locus_tag="WIC03_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEZ3883181.1"
                     /translation="MTTHLSNVAPDLQNYLNAIGEPEHPVLTRLREKTEHHRMGKMAI
                     AREQAAVLVWLAKLIRAEKYLEVGVFTGYSSTVLALALPKHGRITACDINVTFTDTAR
                     QVWNEAGVAHKISLHLQPALLTLDDLIAQGEAGSYDLALIDADKPPTPQYFERCLKLV
                     RQGGIIAIDNILLNGRVMREAASDAPPSVGILKDFNQNLPNDPRIVPITLPVGDGLTL
                     LLKK"
     gene            complement(1981..2166)
                     /locus_tag="WIC03_000643"
     CDS             complement(1981..2166)
                     /locus_tag="WIC03_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HEZ3883182.1"
                     /translation="MYHYQSEATQFLNRLIEEKPELEQQRLENRGLLWDVELNPEEQE
                     NFEAAKVAKKPYTYYQD"
     gene            2309..4285
                     /gene="mutL"
                     /locus_tag="WIC03_000644"
     CDS             2309..4285
                     /gene="mutL"
                     /locus_tag="WIC03_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEZ3883183.1"
                     /translation="MPRIAALPDHLVNQIAAGEVVERPANALKEIVENSIDAGATAID
                     VELAGGGIRLIRVSDNGSGIHPDDIGLALHRHATSKIKTLNDLEHVASMGFRGEGLAS
                     IASVSRLTLTSRQEDSSHATQVKAEDGKLSSPTAAAHPVGTTIEAAELFFNTPARRKF
                     LKSENTEYAHCTTMLERLALAHPHIAFSLKRDGKQVFKLPTQSLPERIAAIVGDDFQT
                     ASLEIDSGNGALRLYGAIAKPTFAKGKTDKQYCFVNHRFVRDKVMLHAVKQAYRDVLH
                     NALTPAFVLFLDLPPEAVDVNVHPTKTEIRFRDSQQVHQLVFHTLNKALADTRANLTE
                     SVGNAGEVLHDITGVVSTPMPSENDSENLFDSASDYPTGNKPDTRNAFGSSGKTAPMP
                     YQAARAPQQHSLSLRESRAAMNTYAELYKKTDDIDLELNRLEQARFGNMPSETPIPKT
                     DTPLSDGLPSQSELPPLGFAIAQLLGIYILAQAEDSLLLIDMHAAAERVNYEKMKRQR
                     RENGKLQSQRLLIPSTFAASHEECAALADHAEALAGFGLELSDMGGNTLAVRTVPAML
                     GKADVVSLAKDVLGELAQVGSSQTIEEHENRILATMSCHGSVRAGRRLTLPEMNALLR
                     DMENTPRSNQCNHGRPTWVKLTLKELDALFLRGQ"
     gene            4380..6509
                     /gene="dnaX"
                     /locus_tag="WIC03_000645"
     CDS             4380..6509
                     /gene="dnaX"
                     /locus_tag="WIC03_000645"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEZ3883184.1"
                     /translation="MAYQVLARKWRPKTFSDLVGQEHVVKALQNALDEGRLHHAYLLT
                     GTRGVGKTTIARILAKSLNCENAQHGEPCGVCESCTQIDAGRYVDLLEIDAASNTGID
                     NIREVLENAQYAPTAGKYKVYIIDEVHMLSKSAFNAMLKTLEEPPEHVKFILATTDPH
                     KVPVTVLSRCLQFVLHNMTSQQVADHLAHVLDSEKIAYEPPALQLLGRAAAGSMRDAL
                     SLLDQAIAMGSGSVAEQDVRQMIGAVDKQYLYELLTGIINQDGATLTAKAQEMAACAV
                     GFDNALGELAILLQHLALIQAVPSALAHDDPDSDILHRLAQTISGEQIQLYYQIAVHG
                     KRDLSLAPDEYAGFMMTLLRMLAFAPLAAASCDTNAVIENTELKSPSAQTAEKETAVK
                     KPQPRPEAETAQTPVQTASAAAMPSEGKTAGPVSNQENNDVPPWEDAPDEAQAAAGTA
                     QTSAKSIQTASEAETPPENQVSKNKAADNVTNAPLSETPSENPIQATPNNEAVETETF
                     AHEAPAEPFYGYGFPDNDCPVEDNAEIPPPDWEHAAPADTAGGGADEEAEAGGIGGNN
                     TPSAPPPEFSTENWAAIVRHFARKLGAAQMPAQHSAWTEYHPDTGLMVLAMTAEARAT
                     ADKKRLDKIRDTLAQAYGLQLTLQTQDWQDEAGRETPAMQDKRVQIEDRQKAQALLEA
                     DPAAQKILQAFGAQWQPESLELAANRP"
     misc_feature    5569..5608
                     /gene="dnaX"
                     /locus_tag="WIC03_000645"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01843"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="This ribosomal frameshift element is known only in
                     the genus Neisseria; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01843"
     gene            6590..6925
                     /locus_tag="WIC03_000646"
     CDS             6590..6925
                     /locus_tag="WIC03_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEZ3883185.1"
                     /translation="MFGKAGLGGLMKQAQQMQENMKKAQAKLAETEIEGEAGNGLVKI
                     TMTCAHEVRKIDISPDLIQEAADDKEMLEDLILAALKSARDKAEETANKTMGAFTQGL
                     PPGVGDFFR"
     gene            complement(7681..8727)
                     /gene="recA"
                     /locus_tag="WIC03_000647"
     CDS             complement(7681..8727)
                     /gene="recA"
                     /locus_tag="WIC03_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEZ3883186.1"
                     /translation="MSDDKSKALAAALAQIEKSFGKGAIMKMDGSQQEENLEVISTGS
                     LGLDLALGVGGLPRGRIVEIFGPESSGKTTLCLEAVAQCQKNGGVCAFVDAEHAFDPV
                     YARKLGVKVEELYLSQPDTGEQALEICDTLVRSGGIDMVVVDSVAALVPKAEIEGDMG
                     DSHVGLQARLMSQALRKLTGHIKKTNTLVVFINQIRMKIGVMFGSPETTTGGNALKFY
                     SSVRLDIRRTGSIKKGEEVLGNETRVKVIKNKVAPPFRQAEFDILYGEGISWEGELID
                     IGVKNDIINKSGAWYSYNGAKIGQGKDNVRVWLKENPEVANEIDAKIRALNGVEMHIT
                     EGTQDETDGERPEE"
     gene            8981..9745
                     /locus_tag="WIC03_000648"
     CDS             8981..9745
                     /locus_tag="WIC03_000648"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEZ3883187.1"
                     /translation="MCSCLVVKNTVIGSGRTKIAVPLVARDAAELSAVLEQIKNMPFD
                     IAEFRADFLECAGSIGEVLRHTQTVRDALPDKPLLFTFRRHGEGGSFPCSDDYYFELL
                     DALIESRLPDIIDIELFSGETAVRRAVANAQKNGIAALLCNHEFHRTPPQEEIVCRLK
                     QMEDCGADICKIAVMPQSAEDVLTLLSATLEAKRLAAKPIVTMSMGQTGTVSRLAGQV
                     FGSSITFGSGTQNSAPGQIGVSALRAALDCLESGAD"
     gene            9765..11780
                     /gene="rep"
                     /locus_tag="WIC03_000649"
     CDS             9765..11780
                     /gene="rep"
                     /locus_tag="WIC03_000649"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HEZ3883188.1"
                     /translation="MMKLNPQQLEAVRYLGGPLLVLAGAGSGKTGVITQKIKHLIVNV
                     GYLPHTVAAITFTNKAAAEMQERVAEMLPKSQTRGLTICTFHSLGMKILREEANHIGY
                     KKNFSILDSTDSAKIIGELLGGTGKEAVFKAQHQISLWKNDLKTPEDVVQTASNAWEQ
                     QTARVYASYQETLQSYQAVDFDDLIRLPAVLLQQNSEVRNKWQRRLRYLLVDECQDTN
                     TCQFALMKLLTGAEGMFTAVGDDDQSIYAWRGANMENLRKMQENYPQMKVVKLEQNYR
                     STARILKIANKVIENNPKLFTKKLWSQLGEGEPVKVVACQNEQHEADWVVSQIIKQKL
                     IGGDKTQYADFAVLYRGKHQARIFEEALRGVRIPYRLSGGQSFFDKAEIKDVLSYVRL
                     LANPNDDPAFLRAVTTPKRGIGDVTLGKLNAYAHEHECSLYEAAQTEEALATLNNTNR
                     QHLQAFMDMFGSYRAKAETGEAGEFINSLLEEIDYENHLLASEEGKAGEIKWRNVGDL
                     VSWFARKGGEDGKNIIELAQTVALMTLLEGKDEEETDAVSLSTLHAAKGLEYPYVFLV
                     GCEEGVLPHNDSIEEGNVEEERRLMYVGITRAKRQLTLTHCVKRKKQGIWQFPEPSRF
                     IDEMPQEDLKILGRKGGEPIVSKEEGRRNLADIIGRLDSMKKGGPED"
BASE COUNT         2902 a         3454 c         3069 g         2386 t
ORIGIN      
        1 aacgccgtac tggtttaaat ttaagccact atatattcgc ggacggcggg ttttaaatct
       61 gttccaattc catactcaaa acagcctgtt cctgtttggc tcggaagtct gccagttttt
      121 gcgccagttc gggggtttcg ttggcgagca tggaaacggc gaacaatgcg gcatttgccg
      181 cgcctgcctc gccgatggcg aatgtggcga cgggtacgcc tttgggcatt tgtacaatcg
      241 ataaaagcga atcttcgccg cgcaggtatt tgctggggac gggtacgccc aaaacgggga
      301 cggtggtctt ggcggcaacc atgccgggca ggtgcgccgc gccgcccgcg cccgcgataa
      361 tggctttgat gccgcgctcc cgtgcggttt cagcgtattg gaacattaaa tccggggtgc
      421 ggtgtgcgga aacgacgcgc gcctcatatt ctacgccgaa ctcttcaaga aactgcgctg
      481 cctgccgcat aacgggccaa tcgctgttgc tgcccatgat gatgccgatt tgaatcataa
      541 atcctccttg gtgcggatgg ggtaaaaagc ggaaaaatgg aaaaactatc gtttgcgtat
      601 ggctgaggcg gcgcgttttg ccgccgggct gcccgggtaa gcctgtatca gactgcgcca
      661 agtcgccctt gcaatgtctt tctgctgcaa cctgtattgg cattcgccga ttttgaatat
      721 ggcttcgggc gcggttgggc tgtctttgaa acggttggcg taacgccctc cgatttcgat
      781 gacggattcg cagttcccca tacgcgccct gctttgcagc aacaggtaca tactgcgttg
      841 cgcgatgctg ccgccgtcgc ctccgtccgc gcctttcaac agggcggcag cggcagaaaa
      901 cctgccgctt ttatagtgtt tgagcgcctg attgtagagg ttttgtgcgg tttcaacggt
      961 atgtgcggat gcgctgccgc cttcggtatt gaggtaatgc tctttcaact tgcggtcatc
     1021 gagtttttgg acgtatgtcc tgccggaagg gtgtgttttt gcgtgttcca gtgctttgac
     1081 tttgccgttt aaggtttcca cttcgttcga cagccggacg attttgcctt ccagatagtc
     1141 caaacggtct tgcagagtgg gaacgggata gggaatagcg tccgaactac ctgcctctgc
     1201 ctgcggttcg ggccgcatat cctgaatatt gcgggaaaca ggggaagaac aggcggcgga
     1261 cacagacagc caaatgataa aaagcggtaa tttggtcttc attatttttt cagaagcagg
     1321 gtcaaaccgt cgccgacggg cagggtgatg gggacgatgc gcgggtcgtt cggcaggttt
     1381 tgattaaaat ctttgaggat gccgacgctg ggcggcgcat cggaagccgc ttcgcgcatc
     1441 acccttccgt tcaacaaaat attgtcgatg gcaatgatgc cgccttgccg gacaagcttg
     1501 aggcaacgct cgaaatattg cggcgtgggc ggtttgtctg cgtctatcag tgccaaatcg
     1561 tagcttccgg cctcaccctg tgcaatcaaa tcatccaatg tcagcaatgc gggttgcaga
     1621 tgcaggctga ttttatgtgc cacgcctgcc tcgttccaaa cctgacgcgc cgtatcggta
     1681 aaggttacat tgatgtcgca ggcggtaatc cgcccgtgtt ttggcaacgc caatgcaagc
     1741 acggtgctgc tgtatccggt aaatacgccg acttccagat atttttccgc gcgaatcagc
     1801 tttgccagcc aaaccaaaac cgccgcctgt tcgcgcgcaa tcgccatttt gcccatacgg
     1861 tgatgctcgg tcttctcacg cagccgcgtc aaaacgggat gttcgggttc gccgatggcg
     1921 ttcaaatagt tttgcaggtc cggtgcgaca ttggacagat gggtcgtcat ttcggtgggt
     1981 tcagtcttgg taataggtat aaggtttttt cgctactttt gccgcctcga agttttcctg
     2041 ttcttcggga ttgagttcga catcccacaa aagcccccgg ttttccaaac gctgctgttc
     2101 caactcaggt ttttcttcaa tcagacggtt gaggaattgt gtggcttcgg attggtagtg
     2161 atacatcttt gcgctccaat tttacgggat acggcgtgat tatactggta ttttccaaac
     2221 gggataaacg gcttttatca agaatacagg cagaaaagta aggggtttta ttatagaata
     2281 aagcgttttt gcaacggaag cctaccttat gccacgcatt gccgccctgc ccgaccatct
     2341 tgtcaaccaa atcgccgccg gcgaagtggt cgaacgcccc gccaacgcct taaaagaaat
     2401 cgttgaaaac agcatagacg caggcgcgac ggcgattgat gtcgaactgg cgggcggcgg
     2461 catccgtctc atccgcgtca gcgacaacgg cagcggtatc catccagacg acatcgggct
     2521 tgcgctccac cgccacgcca ccagcaaaat caaaactcta aacgatttgg aacacgttgc
     2581 cagcatgggc tttcgcggcg aaggtttggc cagtatcgcc tcagtcagcc gcctaaccct
     2641 gaccagccgt caagaagaca gttcgcacgc gacccaagtc aaagcagaag acggaaaact
     2701 cagcagccct acggcagccg cccaccccgt cggcaccacc atcgaagccg ccgaactctt
     2761 cttcaatacc cctgcgcggc gcaagttcct caaatccgaa aacaccgaat acgcccactg
     2821 caccaccatg ctcgaacgcc ttgcactggc gcatcctcac attgccttct cactcaaacg
     2881 cgacggcaaa caagtattca aactcccaac ccaaagcttg cctgaacgga ttgccgccat
     2941 tgtcggcgat gattttcaga cggcttcatt ggaaattgac agcggcaatg gcgcattgcg
     3001 gctctatggc gcaattgcca agccgacttt tgccaaaggc aaaactgaca aacaatactg
     3061 cttcgtcaac catcgttttg tccgcgacaa agtgatgctc cacgccgtca aacaggcata
     3121 ccgcgacgta ttgcacaacg cccttacccc tgctttcgtc ctctttctcg acctgccgcc
     3181 cgaagccgtg gatgtcaacg tccacccgac caaaaccgaa atccgcttcc gcgacagtca
     3241 gcaggtgcac caacttgtgt tccacacgct caacaaagcc cttgccgaca cacgcgccaa
     3301 cctgaccgaa agcgtcggca acgcaggcga agtgttgcat gacattaccg gcgttgtctc
     3361 caccccaatg ccgtctgaaa acgacagcga aaatctgttt gatagcgcat ccgactaccc
     3421 gacaggcaac aaacccgata cacgcaatgc ctttggttca tcaggcaaaa ccgcgcccat
     3481 gccttaccaa gccgcgcgtg cgccgcaaca acacagcctg tccctgcgcg aaagccgcgc
     3541 ggcaatgaat acttacgccg agctttacaa aaaaaccgac gacatcgacc ttgagttgaa
     3601 ccgattagag caggcgcgtt tcggcaatat gccgtctgaa acgcctatcc caaaaacaga
     3661 tacgccgctt tcagacggtc ttccctccca atccgaactg ccgccgctcg gtttcgccat
     3721 tgcccaattg ctaggcatct acatccttgc ccaagccgaa gacagcctgt tgctcatcga
     3781 tatgcacgcc gccgccgaac gcgtcaacta cgaaaaaatg aaacgccaac gtcgggaaaa
     3841 cggcaaactg caaagccaac gcctgcttat tccctcaacc tttgccgcgt cccacgaaga
     3901 atgcgccgcc cttgccgacc acgccgaagc actggcaggc ttcgggctgg aactgtccga
     3961 tatgggcggc aatacccttg ccgtccgcac agttcccgcc atgctcggca aagccgacgt
     4021 cgtctcactc gccaaagacg tattgggcga actcgcccaa gtcggcagca gccaaaccat
     4081 cgaagaacac gaaaaccgca tcctcgccac tatgtcctgc cacggctccg tccgcgccgg
     4141 ccgccggctt accctgccgg aaatgaacgc gctgctgcgc gatatggaaa acacgccgcg
     4201 cagcaaccaa tgcaaccacg gcaggccgac ttgggtcaaa ctgactttga aagaattgga
     4261 cgcactgttc ttgcgcggac aataaaccga aagtgctaga atacgccgcc cgaagccgcc
     4321 gttcagacgg cattccgacg caccgacaga aacatcacga ccgaaaccaa gagaaaaaca
     4381 tggcctatca agttctcgcc cgaaaatggc ggcccaaaac cttttccgac ttagtcggtc
     4441 aggaacacgt cgtcaaagcc ttgcaaaacg ccttggacga aggcaggctg caccacgcct
     4501 acctgctgac cggtacgcgc ggcgtaggca aaaccaccat cgcccgcatc cttgccaaaa
     4561 gcctcaactg cgaaaacgcg caacacggcg aaccttgcgg cgtgtgcgaa agctgcaccc
     4621 aaatcgatgc cggacgctat gtcgacctgc tcgaaatcga cgccgcctcc aacacaggca
     4681 tcgacaacat ccgcgaagta ttggaaaacg cccaatatgc accgactgcc ggcaaataca
     4741 aagtctatat catcgacgaa gtgcatatgc tttccaaaag cgcgttcaac gctatgctca
     4801 aaacgctgga agagccgccc gaacacgtca aattcatcct tgccacgacc gatccgcata
     4861 aagtccccgt taccgtcttg agccgctgcc tgcaatttgt cttgcacaat atgacttcgc
     4921 agcaggttgc cgaccacctc gcccacgtcc tcgacagcga aaaaatcgcc tacgaacccc
     4981 ctgccctgca acttttggga cgcgccgccg ccggatcgat gcgcgatgcc ttgagcctgc
     5041 tcgaccaagc catcgccatg ggttcgggca gcgttgccga acaagacgtc cgccaaatga
     5101 tcggtgcggt tgacaaacaa tacctttacg aactcttgac aggcatcatc aaccaagacg
     5161 gcgcaaccct gaccgccaaa gcgcaggaaa tggcggcgtg tgccgtcggc tttgacaacg
     5221 ccttgggcga acttgccata ctgctgcaac acctcgccct gatacaggcg gtgccctccg
     5281 ccttggcgca cgacgacccc gattccgata ttttgcaccg cctcgcccaa accataagcg
     5341 gcgaacaaat ccagctttac taccaaatcg ccgtccacgg caaacgcgac ctgagcctcg
     5401 cccccgacga atacgccggc tttatgatga ccctgctgcg tatgctggcg tttgcgccct
     5461 tggcggcagc ttcgtgtgat acaaacgccg tgattgaaaa taccgaacta aaatccccat
     5521 cggcacaaac cgccgaaaag gaaaccgccg taaaaaagcc ccaaccgcgc cctgaagcag
     5581 aaaccgccca aacacccgtt cagacggcat ccgcagcggc aatgccgtct gaaggcaaaa
     5641 ctgccggacc cgtttccaat caagaaaaca acgatgttcc gccttgggaa gacgcgccag
     5701 acgaagccca agccgcagcc ggcacggcgc aaacgtcggc aaaaagcatt cagacggcat
     5761 ccgaagccga aacgccgccc gaaaaccaag tttccaagaa caaggcagcc gacaacgtaa
     5821 ccaatgcccc cttgtccgaa acgccgtctg aaaaccccat tcaggcaaca ccgaataatg
     5881 aagccgttga aacggaaaca tttgcacacg aagcccctgc agaacctttc tacggttacg
     5941 gctttccgga taatgactgc cccgtagaag acaacgcaga aatcccgccg cccgattggg
     6001 aacacgccgc ccctgccgat accgcaggcg gaggcgcgga cgaagaagca gaagcgggcg
     6061 gcatcggcgg aaacaacacg ccgtccgccc cgccgcccga attttccacc gaaaactggg
     6121 cagccatcgt ccggcatttc gcccgcaaac tcggcgcggc gcaaatgccg gcgcaacact
     6181 ccgcgtggac ggaataccat cccgacaccg gcctgatggt tttggcgatg accgccgaag
     6241 cacgcgccac cgccgacaaa aaacgcctcg acaaaatccg cgacaccctt gcccaagcct
     6301 acgggctgca actcaccctg caaacccaag actggcagga cgaagccggc agagaaaccc
     6361 ccgcaatgca ggacaagcgc gtccaaatcg aagacaggca aaaagcacaa gcattgctcg
     6421 aagccgaccc cgccgcacaa aaaatcctcc aagcattcgg cgcgcaatgg cagcccgaat
     6481 cactggaatt ggcggcaaac cggccataaa cagatataat gccgcccgaa cccttcggac
     6541 ggcattgccg tttccctcat tcattcaaac acaaacagga gtattcagta tgttcggaaa
     6601 agccggatta ggcggcctga tgaaacaggc gcagcaaatg caggaaaata tgaaaaaagc
     6661 gcaagccaaa cttgccgaaa ccgaaatcga aggcgaagca ggcaacggct tggtcaaaat
     6721 cacaatgacc tgcgcgcacg aagtacgcaa aatcgacatc agccccgatt tgattcaaga
     6781 agccgccgac gacaaagaaa tgctcgaaga cctcatcctc gccgccctca aatccgcccg
     6841 tgacaaagcc gaagaaaccg caaacaaaac aatgggcgca ttcacgcaag gtctaccccc
     6901 cggagtgggc gacttcttcc gctgataccc aaccgtcatt cccacgcagg cgggaatcta
     6961 gaacgtagaa tctaagaaac cgttttactc gataaatttc cgtgccgacg ggtctagatt
     7021 cccgccttcg cgggaatgac ggcatcagtt tgcaggattc ggcgtgaacg gtaaaaacag
     7081 tgagaatgat aagaacgcaa aaacggcaag aatagcggga atcggcaggc tgaagcccac
     7141 cctaccatta tttacacatc cgtaccgctt aaatgccgtc tgaaacttcg tcattcccgt
     7201 gaaagcggga atccaacccc gtcggagcag aaacttacac cccgtcattc ccgcgaacgc
     7261 gggaatccag taaccgaaaa accacaggaa tctatcggaa aaacagaaac cctcgaccgt
     7321 cattcccgcg aacgcgggaa tctagaacgt agaatctgag aaaccgtttt actcgataaa
     7381 tttccgtgcc gacaggtttg gattcccgcc ttcgcgggaa tgacggcata ttttttgcat
     7441 ttgatataaa ggggcgtttg aattttgttc agcaagtgca aagtgttgca cataaaaagg
     7501 cgcaggcttc atccgtaaag tcggcgcagg atagaggcaa agcgggcgta ggtcgggctg
     7561 tagcaactgt atttttcacc ccgtcgggca aaaataccaa aactcaaatc aagccgttcg
     7621 gaggcggctc aaaaaaacgg tacttcgcag cagaagtacc gtttatcggg atttcaggtt
     7681 ttattcttcg gggcgttcgc cgtcggtttc gtcctgcgtc ccttcggtga tgtgcatttc
     7741 tacgccgttg agggcgcgga tttttgcgtc gatttcattg gcgacttcgg gattttcctt
     7801 cagccagacg cggacgttgt ctttgccctg accgattttc gcgccgttgt agctgtacca
     7861 cgcgccggat ttgttgatga tgtcgttttt cacgccgatg tcgatcaatt cgccttccca
     7921 actgatgcct tctccgtaga ggatgtcaaa ctctgcctga cggaacgggg gggcgacttt
     7981 gtttttgatg actttgacgc gggtttcgtt gcccaatacc tcttcgcctt ttttgatgga
     8041 tccggtgcgg cggatgtcga ggcggacgga agaatagaat ttcagcgcgt tgccgccggt
     8101 ggtggtttcg gggctgccga acattacgcc gatcttcatc cggatttggt tgatgaacac
     8161 aaccagcgtg ttggtttttt tgatgtgtcc ggtcagtttg cgcaaagcct ggctcatcag
     8221 gcgcgcctgc agtccgacat ggctgtcccc catatcgcct tcgatttcgg ctttggggac
     8281 gagtgcggct acggaatcga cgactaccat atctatgccg cccgaacgga cgagtgtgtc
     8341 gcagatttcc aaagcctgtt cgccggtatc gggctgggac aggtaaagct cttcgacttt
     8401 tacgccgagt ttgcgggcgt aaacgggatc aaaggcgtgt tcggcatcga caaaggcgca
     8461 cacgccgccg tttttctggc attgggcgac ggcttcgagg cagagggtgg ttttgccgga
     8521 ggattcgggg ccgaagattt cgacgatgcg cccgcgcggc agaccgccga ctccgagggc
     8581 gaggtctaat ccgagcgatc cggtggaaat gacttcgagg ttttcttcct gctggctgcc
     8641 gtccattttc atgatggcgc ctttgccgaa acttttttcg atttgcgcca gtgcggcggc
     8701 aagggctttg cttttgtcgt ctgacattgg ggttactccg gaacaaatgc ggtatgtggg
     8761 atgcggcgca acacgggctg cggcgcggga tgtgtatcgt tttcccgatg tgcgggctat
     8821 cggtaatgct gcttcacggg gttgccatta tcgcatattt ccttgcttgc cgatatgcgg
     8881 caggacgcgg cggcttgtgc cggaatggaa tctggatgcc gtctaaaagg cggccggctt
     8941 tgttataatg gcggctgttt tttctgtgtg tgcctgtttt atgtgttcct gccttgttgt
     9001 caaaaatacc gttatcggaa gcggacgcac caaaatcgcc gtgccgcttg tcgcccgcga
     9061 tgctgccgaa ctttccgccg tacttgagca aatcaaaaat atgcccttcg atattgcgga
     9121 gttccgcgcc gactttttgg aatgcgcggg cagcatcggc gaagtattgc gccacacgca
     9181 gaccgtccgc gacgcgctgc ccgacaaacc gctgctgttt acgttcagac ggcatggcga
     9241 aggcggctcg ttcccgtgtt cggacgatta ttattttgaa ctgctcgacg cgctgatcga
     9301 aagccgcctg cccgacatca tcgacatcga gctgttttcc ggcgaaaccg ccgtccggcg
     9361 cgccgtggca aatgctcaaa aaaacggcat cgccgcccta ctctgcaatc atgagtttca
     9421 ccgcacgccg ccgcaagaag aaatcgtatg ccgtctgaaa cagatggagg actgcggcgc
     9481 ggacatctgc aaaattgcgg tgatgccgca aagcgcggaa gatgtgctga ctttgctttc
     9541 cgccacgctc gaagcgaaac ggcttgccgc caaaccgatt gttacaatgt cgatgggaca
     9601 gacggggacg gtcagccggc ttgccggaca ggtgttcggc tcaagcatca cgttcggttc
     9661 gggaacgcaa aactccgcgc cggggcaaat cggcgtatcc gccctccgtg cggcactcga
     9721 ctgtctcgaa agcggcgcag actgatttca gacggcatca aaacatgatg aaactcaatc
     9781 cccaacagct cgaagccgtc cgttacctcg gcggccccct gctcgtcctt gccggtgcag
     9841 gcagcggcaa aaccggcgta attactcaaa aaattaagca tttgattgtc aatgtcggct
     9901 atctgccgca taccgtcgcc gcaattacct ttaccaacaa agccgctgcg gaaatgcagg
     9961 agcgcgtcgc cgaaatgctg cccaagtcgc aaacgcgcgg gctgacaatt tgcacgttcc
    10021 attctttggg catgaagatt ttgcgcgaag aggcgaacca tatcggttac aaaaaaaact
    10081 tttccatcct cgattccacc gacagcgcga aaatcatcgg cgaacttttg ggcggtacgg
    10141 gcaaagaagc cgtattcaag gcgcagcacc agatttcctt gtggaaaaac gatttaaaaa
    10201 cgcctgaaga tgtcgttcag acggcctcta atgcgtggga acaacaaacc gcacgcgtgt
    10261 atgcgagcta tcaggaaacc ttacaaagct atcaggcagt ggacttcgat gacttaatcc
    10321 gcctgcctgc cgtgctgttg cagcaaaaca gcgaagtgcg caacaaatgg cagcggcggc
    10381 tgcgttatct gctggttgac gaatgccaag atacgaatac ctgccaattt gcgctgatga
    10441 aactcttgac cggtgcggaa ggtatgttta ccgccgtcgg cgacgacgac cagtccatct
    10501 acgcgtggcg cggtgcgaat atggaaaacc tgcgcaaaat gcaggaaaac tatccgcaga
    10561 tgaaggtcgt caaactggag caaaactacc gctccaccgc gcggattctt aaaatcgcca
    10621 acaaagtcat cgaaaacaac cccaagctgt ttaccaaaaa actttggtcg caattgggcg
    10681 aaggcgagcc ggtcaaagtc gttgcctgcc aaaacgagca acacgaagcc gactgggtcg
    10741 tcagccaaat catcaaacaa aaactcatcg gcggcgacaa aacccaatac gccgatttcg
    10801 ccgtgttata ccgcggaaag catcaggcga ggattttcga ggaagcattg cgcggcgtgc
    10861 gcatccccta ccggctctcc ggcggacaaa gctttttcga caaagccgaa atcaaagacg
    10921 tgttgtctta tgtgcggctg cttgccaacc ccaacgacga tcccgccttt ctgcgcgccg
    10981 taaccacgcc caaacgcggc atcggcgatg taacgctggg caagctcaac gcctacgcgc
    11041 acgaacacga atgcagcctg tatgaagccg cgcaaaccga agaagccctt gccacgctga
    11101 acaataccaa ccgccaacac ctgcaagcct ttatggatat gttcggcagc taccgcgcca
    11161 aagccgaaac aggcgaagca ggcgagttca tcaacagcct gctcgaagaa atcgactatg
    11221 aaaaccacct gcttgccagt gaagaaggca aagctggcga aatcaaatgg cgcaacgtcg
    11281 gcgatttggt atcatggttt gcgcgaaaag gcggggaaga cggcaaaaac atcatcgaac
    11341 tcgcccaaac cgtcgccttg atgacgcttt tggaaggaaa agacgaagaa gaaaccgatg
    11401 ccgtctcgct atccacgcta cacgccgcca aaggtttgga gtatccctat gttttccttg
    11461 tcggttgcga agaaggtgtt ttgccgcaca acgacagcat cgaagaaggc aacgtcgaag
    11521 aagaacgccg cctgatgtac gtcggcatca cccgcgccaa acgccagctt acgctgaccc
    11581 actgcgtcaa acgcaaaaaa caaggcatat ggcagttccc cgaacccagc cgattcatag
    11641 acgaaatgcc gcaggaagat ttgaaaatcc tggggcgcaa aggcggcgaa ccgattgtca
    11701 gcaaagaaga aggcagacgc aaccttgccg atataatcgg aaggctcgac agcatgaaaa
    11761 aaggcggtcc ggaggattaa accaagatag ggaaacgaag ccggagacaa t
//
LOCUS       DAWHSK010000035        11732 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.035, whole genome shotgun sequence.
ACCESSION   DAWHSK010000035 DAWHSK010000000
VERSION     DAWHSK010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11732
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.035"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(70..1074)
                     /gene="gap"
                     /locus_tag="WIC03_000650"
     CDS             complement(70..1074)
                     /gene="gap"
                     /locus_tag="WIC03_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003766473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEZ3883189.1"
                     /translation="MSIKVAINGFGRIGRLALRQIEKAHDIEVVAVNDLTPAEMLLHL
                     FKYDSTQGRFQGTAELKDDAIVVNGKEIKVFANPNPEELPWGELGVDVVLECTGFFTN
                     KTKAEAHIRAGARKVVISAPGGNDVKTVVYGVNQDILDGSETVISAASCTTNCLAPMA
                     AVLQKEFGVVEGLMTTIHAYTGDQNTLDAPHRKGDLRRARAAALNIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPVATGSLTELVSVLERPVTKEEINAAMKAAASESYGYNEDQ
                     IVSSDVVGIEYGSLFDATQTRVMTVGGKQLVKTVAWYDNEMSYTCQLVRTLEYFAGKI
                     "
     gene            1516..4110
                     /gene="mutS"
                     /locus_tag="WIC03_000651"
     CDS             1516..4110
                     /gene="mutS"
                     /locus_tag="WIC03_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEZ3883190.1"
                     /translation="MSKSAVSPMMQQYLGIKAQHTDKLVFYRMGDFYEMFFDDAVEAA
                     KLLDITLTTRRQVDGEPVKMAGVPFHAAEQYLARLVKLGKSVAICEQVGEVGAGKGPV
                     ERKVVRIVTPGTLTDSALLEDKETNRIVAVSPDKKYIGLAWASLQSGEFKTKLTTVDK
                     LDDELARLQAAEILLPDSKNAPQLQTASGVTRLNAWQFAADAGEKLLTEYFGCQDLRG
                     FGLDGKEHAVAIGAAGALLNYIRLTQNLMPQHLDGLSLETDSQYIGMDAATRRNLEIT
                     QTLSGKKSPTLMSTLDLCATHMGSRLLALWLHHPLRNRAHIRARQEAVAALESQYKPL
                     QCRLKNIADIERIAARIAVGNARPRDLASLRDSLFELAQIDLSANGSSLLETLKAVFP
                     ENLSTAEQLRQAILPEPSVWLKDGNVINHGFHPELDELRRIQNHGDEFLLDLEAKERE
                     RTGLSTLKVEFNRVHGFYIELSKTQAEQAPADYQRRQTLKNAERFITPELKAFEDKVL
                     TAQEQALALEKQLFDGVLKNLQTALPQLQKAAKAAAALDVLSTFSALAKERNFVRPEF
                     ADYPAIHIENGRHPVVEQQVRHFTANHTNLDHKHRLMLLTGPNMGGKSTYMRQVALIV
                     LLAHTGCFVPADAATIGPIDQIFTRIGASDDLASNRSTFMVEMSETAYILHHATEQSL
                     VLMDEVGRGTSTFDGLALAHAVAEHLLQKNKSFSLFATHYFELTYLPEAHAAAVNMHL
                     SALEQGQDIVFLHQIQPGPAGKSYGIAVAKLAGLPVRALKSAQKHLNELENQAAANRP
                     QLDIFSTMPSEKGDEPNVGNFVDKAEEKHFEGILAAALEKLDPDSLTPRKALSELYRL
                     KDLCKSVS"
     gene            complement(4233..4724)
                     /locus_tag="WIC03_000652"
     CDS             complement(4233..4724)
                     /locus_tag="WIC03_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ3883191.1"
                     /translation="MNSLFVDNTVFITRLKAGHIGRLVQALFEEWHGFEPWSSVDKIH
                     AYYGRCLKDDELPLAFAAVDDSGTLLGSATVKRHDMESFPQYEYWLGDVFVLPEYRGK
                     GIGRRLVAHCIGAARSLGIKFLYLYTPDVQIFYESFGWAVVGRHFHNGEWVTVMRLDM
                     DKV"
     gene            complement(4728..5123)
                     /locus_tag="WIC03_000653"
     CDS             complement(4728..5123)
                     /locus_tag="WIC03_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883192.1"
                     /translation="MPSDVGMRLQTAFKRKLNMKKILYFLMFVFSTSVWAGDAEDNLL
                     SIQSGYRALLQKQNNLDGKIIGMQSDLEDARRRLQTAQADIARLEAEIPAAMAQKARQ
                     AEELRQIGVRLDYAWNAVYGAGGTKASGN"
     gene            complement(5144..6538)
                     /locus_tag="WIC03_000654"
     CDS             complement(5144..6538)
                     /locus_tag="WIC03_000654"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEZ3883193.1"
                     /translation="MTVKTRFAPSPTGYLHIGGVRTALFSWAFARHHKGEFLLRIEDT
                     DLARSTAESVNIILDGMKWVGLDYDNADNVVYQTRRFERYKEVIAELLAKGDAYYCYC
                     SKEELEAMREKAEKEGTATYDRRWRPEAGKTLPEIPAGVQPVVRFKTPLDGVTKWTDL
                     VKGEISIPNEALDDLIIARADGTPTYNFCVVVDDYDMGVTHVIRGDDHVNNTPKQINI
                     LKAIGANLPEYGHLPMILNEQGKKISKRSGDTVAITDFGAMGILPEAMLNYLARLGWA
                     HGDDEFFTMEQFIEWFDLKDVSPSPSRMDLKKLYWINGEHIKITPNEKLAELVKPRLA
                     LRDIHETEKPALEDVLAQVKDRAQDLNALADECLYFYKKQVPAEADVAKHWDDEAAAR
                     MLRFAERLEGLEDWNAEAIHDLFKPFCDEEGIKMGKLGMPLRLAVCGTAKTPSVDAVL
                     ALIGKEEVLKRIRA"
     gene            complement(6742..7386)
                     /locus_tag="WIC03_000655"
     CDS             complement(6742..7386)
                     /locus_tag="WIC03_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEZ3883194.1"
                     /translation="MNKRLFCSRNGLRYYLLGGFCLSVFPFLLVFASSVWAVYRTGGQ
                     VLPPYVRADAALVLGAAAWDKRPSPVFRERINHAIALYQSRRVGKIVFTGGRTKKGYM
                     TEAEVGRRYALKQGVPARNILFENTSRNTYENLNNIRPVLRANGIASVVIVSDPYHLA
                     RAAEMAEDLGVRVYMSATPTTRFDAGNKKKIFMLQEGYALSLYRLEKWGRDFFN"
     gene            complement(7383..7736)
                     /gene="arsC"
                     /locus_tag="WIC03_000656"
     CDS             complement(7383..7736)
                     /gene="arsC"
                     /locus_tag="WIC03_000656"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271974.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEZ3883195.1"
                     /translation="MSEIKIFHNPRCSKSRAALSLLEERGIAAEVVKYLDTPPDLSEL
                     KDIFNKLGLASARGMMRVKDDLYKELGLDNPNLDNDALLRAIADHPALLERPIVLANG
                     KAAVGRPLENIEAVL"
     gene            7860..8342
                     /locus_tag="WIC03_000657"
     CDS             7860..8342
                     /locus_tag="WIC03_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEZ3883196.1"
                     /translation="MKYLNLAAITLAATFAAHTASADELAGWKDNTPQSLQSLKAPVR
                     IVNLWATWCGPCRKEMPAMSKWYKAQKKGSVDMVGIALDTSDNIGNFLKQTPVSYPIW
                     RYTGANSRNFMKSYGNNVGVLPFTVVEAPKCGYKQTITGEVNEKSLTEAVKLAHSKCR
                     "
     gene            8350..8541
                     /locus_tag="WIC03_000658"
     CDS             8350..8541
                     /locus_tag="WIC03_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883197.1"
                     /translation="MPSESASDGIFPARPSVSAKLIHYLKTGGIFIIGTVLPIVQTAY
                     PCGRNRPKRYAAHSLQDLL"
     gene            8538..9188
                     /gene="ftsE"
                     /locus_tag="WIC03_000659"
     CDS             8538..9188
                     /gene="ftsE"
                     /locus_tag="WIC03_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEZ3883198.1"
                     /translation="MIRFEQVSKTYPGGFEALKNVSFQINKGEMIFIAGHSGSGKSTV
                     LKLISGITKPSRGKILFNGQDLGTLSDNQIGFMRQHIGIVFQDHKILYDRNVLQNVIL
                     PLRIIGYPPRKAEERARIAIEKVGLKGRELDDPVTLSGGEQQRLCIARAVVHQPGLLI
                     ADEPSANLDRAYALDIMELFKTFHEAGTTVIVAAHDETLMADYGHRILRLSKGRLA"
     gene            9185..10102
                     /gene="ftsX"
                     /locus_tag="WIC03_000660"
     CDS             9185..10102
                     /gene="ftsX"
                     /locus_tag="WIC03_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HEZ3883199.1"
                     /translation="MSIIHYLSLHVESARTALKQLLRQPFGTLLTLIMLAVAMTLPLF
                     MYLGIQSGQSVLGKLNESPQITIYMETAAAQSDSDTVRSLLARDKRLDNIRFIGKEDG
                     LEELQSNLDQNLISMLDGNPLPDVFIVTPDPATTPAQMQAIYRDITKLPMVESANMDT
                     EWVQTLYQINEFIRKILWFLSLTLGMAFVLVAHNTIRLQILSRKEEIEITKLLGAPAS
                     FIRRPFLYQAMWQSILSAAVSLGLCGWLLSAVRPLVDAIFKPYGLNIGWRFFYPGEIA
                     LVFGFVIALGVFGAWLATTQHLLGFKAKK"
     gene            10193..10441
                     /locus_tag="WIC03_000661"
     CDS             10193..10441
                     /locus_tag="WIC03_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HEZ3883200.1"
                     /translation="MLTSEQVKAMIEGVAKCEHIEVEGDGHHFFAVIVSSEFEGKARL
                     ARHRLIKDGLKAQLESNELHALSISVAATPAEWAAKAQ"
     gene            complement(10508..11686)
                     /locus_tag="WIC03_000662"
     CDS             complement(10508..11686)
                     /locus_tag="WIC03_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEZ3883201.1"
                     /translation="MAFLKLTEQNVQGKTVLIRADMNVPFKDGKISDDTRIRASIASV
                     KYCVDNGASVIVMTHLGRPTEGEFHPEDDVAPVAAHLGGLLGKNVKVLNDWRENKPAL
                     NAGDVVMLQNVRINKGEKKNDLELGKAYASLCDVFVNDAFGTAHRAQASTEAVAQAAP
                     VACAGVLMAGELDALGKALKQPARPMVAIVAGSKVSTKLTILESLADKVDQLIVGGGI
                     ANTFLLAEGKAIGKSLAEHDLVEESKKIMEKMAAKGGSVPLPTDVVVAKAFAADAEAV
                     VKDIADVAEDEMILDIGPKSAAALAELLKAAGTVVWNGPVGVFEFDQFAGGTKALAEA
                     IAQSKAFSIAGGGDTLAAIAKFGVTDQIGYISTGGGAFLEFLEGKELPAVAALEKRGA
                     "
BASE COUNT         2809 a         3378 c         2871 g         2674 t
ORIGIN      
        1 agataaacaa tgccgtctga acgcaatgtg ttcagacggc atttacttat ccacaggttt
       61 gttcaagcct tagattttgc ctgcaaagta ttccaaagtg cggacgagtt ggcaggtgta
      121 ggacatttcg ttgtcgtacc aggcaacagt tttcaccaat tgtttgccgc ccacggtcat
      181 cacgcgggtt tgggtcgcat cgaagagcga accgtattcg atgccgacaa cgtcggaaga
      241 aacaatttga tcttcgttgt agccgtaaga ttcgctggcg gcggctttca tcgcggcgtt
      301 gatttcttct ttggttacag ggcgttcgag gacggaaacc aattcggtca gcgagccggt
      361 ggcaacaggg acgcgttggg cggagccgtc gagtttgccg ttcaattcag ggataaccag
      421 accgatggcc ttggcggcac cggtgctgtt gggcacgatg ttgagcgcgg cggcgcgggc
      481 gcggcgcaaa tcgcctttgc ggtgcggcgc gtcaagggtg ttttggtcgc cggtgtaggc
      541 gtggatggtg gtcatcaggc cttcgactac gccgaattcc ttttgcagga ctgccgccat
      601 cggggcaagg cagttggtgg tgcaggaagc agcggagata acggtttcgc tgccgtccaa
      661 aatgtcttgg tttacgccat atacgacggt tttcacatca ttgccgccgg gtgcggaaat
      721 cacgactttg cgcgcgccgg ccctgatgtg tgcttcggct ttggttttat tggtaaagaa
      781 gccggtacat tcgaggacga catccacacc caactcgccc caaggcaatt cttcgggatt
      841 cggattggca aaaactttga tttctttgcc gtttaccacg atggcatcgt cttttaattc
      901 ggcagtacct tggaaacggc cttgcgtgct gtcgtatttg aaaaggtgca gcagcatttc
      961 ggcaggggtc aggtcgttga cggcgacgac ttcgatgtcg tgggcttttt caatttgacg
     1021 caatgcgagg cggccgatgc ggccgaaacc gttaatcgct actttaatgc tcatattttg
     1081 tctccaggtt gtgaaataaa gtccgatgcc tgcattgtat tctgaaataa agttacattc
     1141 cactattaca tctaactact tgacgcttat ttgatataga tgaattttgc tgtttgcaca
     1201 gatttccaaa acctttacca tcaatatttg aatttaaatt ttaatgatga ttttgatgat
     1261 tgccaacctg cttgtgcgta agtagcaaat atccaatatt ttcattatct ttttgtcaaa
     1321 taagtttgag tttaagactt gctgtataag acagataagc gtggatgttt tttgacttaa
     1381 taatatttct gtggataact ttgctgtttt cctagttgtc tccacaacct tattgacagg
     1441 cttacggtca gtctcattcc gtcgaagaca aaaccttttg ctacaatacc gttttcctaa
     1501 tgataaggca gccccatgtc caaatccgcc gtttccccaa tgatgcagca atacctcggc
     1561 atcaaagcgc aacataccga caaactggtg ttttaccgta tgggcgattt ttacgagatg
     1621 tttttcgacg atgcggtaga agcggcaaaa cttttggata ttaccctgac cacgcgcaga
     1681 caggtggatg gcgagccggt caaaatggca ggcgtgccgt ttcacgccgc cgaacaatat
     1741 ctggcgcgcc tggtcaagtt gggcaaaagc gtggcgattt gcgaacaggt cggcgaagtc
     1801 ggcgcgggca aagggcctgt ggagcgcaaa gtcgtgcgca tcgtaacgcc cggcacgctg
     1861 accgattccg cattgctgga agacaaggaa accaaccgta tcgttgccgt gtcccccgac
     1921 aaaaaataca tcggtttggc gtgggcatcg ctgcaaagcg gcgaattcaa aaccaagctg
     1981 acaactgtgg ataaattgga cgacgaactg gcgcgcctgc aggcggcgga aattctgttg
     2041 ccggacagta aaaacgcacc gcaacttcag acggcatcgg gtgttacgcg cctgaacgcg
     2101 tggcagtttg ccgccgacgc gggggaaaaa ctgctgacgg aatatttcgg ctgccaggat
     2161 ttgcgcggct tcggtttgga cggcaaagaa cacgccgttg cgattggcgc ggcaggtgca
     2221 ctgttgaact atatccgtct gacgcaaaac ctgatgccgc aacatttgga cggcctgtcg
     2281 ctcgaaaccg acagccaata tatcggtatg gatgctgcca cgcgccgcaa tctcgaaatc
     2341 acgcaaaccc tctccggcaa aaaatcgccg accctgatgt ccacgctcga cctttgcgct
     2401 acccatatgg gcagccgcct cttggctctc tggctgcacc accctttacg caaccgcgcc
     2461 cacatccgag cgcgccaaga agccgttgcc gcgctggaaa gccaatacaa acccctccaa
     2521 tgccgtctga aaaacattgc ggacattgaa cgcattgctg cccgcatcgc cgtcggcaac
     2581 gcccgcccgc gcgatttggc atctttacgc gacagtctgt tcgagttggc acaaatcgac
     2641 ttgtccgcaa acggcagcag cctgttggaa accctcaaag ccgttttccc ggaaaaccta
     2701 tccacagccg aacagctccg ccaagccatt ttgcccgaac cttccgtctg gctgaaagac
     2761 ggcaatgtca tcaaccacgg ttttcatccc gaactggacg aattgcgccg cattcaaaac
     2821 catggcgacg aatttttgct ggatttggaa gccaaggaac gcgaacgtac cggtttgtcc
     2881 acactcaaag tcgagttcaa ccgcgttcac ggcttttaca ttgaattgtc caaaacccaa
     2941 gccgaacaag cacctgccga ctaccaacgc cggcaaaccc tcaaaaacgc cgaacgcttc
     3001 atcacgccgg agttgaaagc ctttgaagac aaagtgctga ctgctcaaga gcaagccctc
     3061 gccttagaaa aacaactctt tgacggcgta ttgaaaaacc ttcagacggc attgccgcag
     3121 cttcaaaaag ccgccaaagc cgccgccgcg ctggacgtat tgtccacatt ttcagccttg
     3181 gcaaaagagc ggaacttcgt ccgccccgag tttgccgact atccggctat ccacatcgaa
     3241 aacggccgcc atcccgttgt cgaacagcag gtacgccact tcaccgccaa ccacaccaac
     3301 cttgaccaca aacaccgcct catgctgctc accggcccca atatgggcgg caaatccacc
     3361 tacatgcgcc aagtcgcgct gattgtttta ttggcacaca ccggctgttt tgtgcctgcc
     3421 gatgccgcca caatcgggcc catcgatcaa atcttcaccc gcatcggcgc atcggacgac
     3481 ctcgcctcca accgctccac tttcatggtc gaaatgagcg aaaccgccta catcctgcat
     3541 cacgccaccg aacaaagcct tgttttaatg gacgaagtcg gacgtggcac ttccactttc
     3601 gacggcctcg ccctcgcgca cgccgttgcc gaacacctgc tgcaaaaaaa caaatccttc
     3661 agcctgtttg ccacccacta tttcgagctg acctacctgc ccgaagccca cgccgccgcc
     3721 gtcaatatgc acctttccgc gctcgaacag ggacaggaca tcgttttcct gcaccaaatc
     3781 caaccgggtc ccgccggtaa aagctacggc attgccgtcg ccaaactcgc cggcctgcct
     3841 gtacgcgcat tgaaatccgc ccaaaagcat ttgaacgaac tggaaaacca agccgccgcg
     3901 aaccgtcccc aactggatat tttcagtacc atgccgtctg aaaaaggaga tgaaccgaat
     3961 gtgggcaact ttgtggataa agcagaggaa aaacattttg aaggtatatt ggcagcagcc
     4021 ttggaaaaac tcgatcccga cagcctgacc ccgcgcaaag cattgtcaga actgtaccgt
     4081 ctgaaagatt tgtgcaaatc cgtatcttaa tttccgttgg cggaacagca tcaaaccata
     4141 tggaaaaatc tgtggataaa cattatctga caagaagttt ccaaacataa aaatgccgtc
     4201 cgaacagctc agacggcatt tgtccattcg gcttaaacct tatccatatc caaacgcata
     4261 accgtaaccc attcaccgtt atggaaatgt cgcccgacaa ccgcccagcc gaatgattca
     4321 taaaatattt gcacatcagg cgtataaaga tacaagaact ttatccccag cgaacgcgct
     4381 gcgcctatgc agtgggcgac cagcctcctg ccaatgcctt ttccgcgata ttcaggtaaa
     4441 acaaagacat cacccaacca atattcatac tgtggaaaac tttccatatc atgccgcttg
     4501 accgtagccg aacccaacag ggttccggaa tcatccacag ccgcaaaagc cagcggcagt
     4561 tcgtcatcct tcaaacacct gccgtaatag gcatgaatct tatccacaga agaccacggt
     4621 tcaaatccgt gccactcctc aaacaacgcc tgaaccaacc tgccgatatg cccggctttc
     4681 agccgtgtaa tgaaaacagt attgtccaca aagagggaat tcatcggtca attccccgac
     4741 gctttcgttc cccctgcgcc gtaaaccgca ttccaagcat agtccaaacg cactccgatt
     4801 tgcctcagct cttcagcctg ccgggctttt tgcgccattg ctgcaggaat ttccgcttcc
     4861 aaacgggcga tgtctgcctg agccgtctgc aaacgccggc gcgcatcttc caaatccgac
     4921 tgcatcccga tgatttttcc gtccagattg ttttgttttt gcagtaaggc acgatagccg
     4981 gattggatgc tgagcagatt gtcttccgca tcccccgccc atacgcttgt agaaaaaaca
     5041 aacatcagaa aatacaatat ttttttcatg ttcaacttcc gtttgaatgc tgtctgaagc
     5101 cgcattccga catcagacgg catcgcccac gcctgtggat aacttaagcg cggatgcgtt
     5161 tcaacacttc ttctttgccg attaatgcca acacagcatc gacgcttggg gttttcgccg
     5221 taccgcagac ggcgaggcgc aggggcatac cgagtttacc cattttgatg ccttcttcgt
     5281 cgcagaaagg tttgaagagg tcgtggatgg cttcggcatt ccagtcttcc aaaccctcga
     5341 ggcgttcggc aaagcgcagc atacgagcgg cggcttcgtc gtcccagtgt ttggcaacat
     5401 cggcttcggc agggacttgt tttttgtaga aatacagaca ttcgtctgcc aaggcgttca
     5461 aatcttgggc gcggtcttta acctgtgcca acacgtcttc caacgcaggt ttttcggttt
     5521 catggatatc gcgcagagcg agacgcggtt tgacgagttc ggcgagtttc tcgttgggcg
     5581 tgattttgat gtgttcgccg ttgatccaat agagcttttt caaatccata cggcttgggg
     5641 acggggaaac gtctttcaaa tcaaaccatt cgatgaattg ttccatcgta aagaactcgt
     5701 cgtcgccgtg cgcccagccc aagcgcgcca gatagttgag catggcttcg ggcaggatac
     5761 ccatcgcgcc gaaatcggta atggcaacag tatcgccgct gcgtttggag atttttttgc
     5821 cttgttcgtt aagaatcatg ggcagatgac cgtattcggg caggtttgcg ccgatggctt
     5881 ttaagatgtt gatttgtttc ggggtgttgt tcacatggtc gtcgccgcgg ataacgtggg
     5941 taacgcccat gtcgtagtcg tctacgacaa cgcagaagtt gtaggtcggt gtgccgtcgg
     6001 cgcgtgcaat aatcaggtcg tcgagggctt cgttggggat ggaaatttcg cctttgacca
     6061 ggtccgtcca cttggttacg ccatccaaag gcgttttgaa gcggacgacg ggttgcacgc
     6121 cggcagggat ttcgggcagg gttttgcctg cttccggccg ccagcggcgg tcgtaagtcg
     6181 ccgtgccttc tttttcggct ttctcgcgca tcgcttccag ctcttctttg ctgcaatagc
     6241 agtagtaggc atcgcctttt gccaagagtt cggcgatgac ttctttatag cgttcgaagc
     6301 ggcgggtttg gtacaccacg ttgtcggcgt tatcgtaatc gagaccgacc catttcatgc
     6361 cgtcgaggat gatgttgacg gattcggcgg tggaacgcgc caagtcggtg tcttcgatac
     6421 gcaataggaa ctcgcctttg tgatggcggg caaacgccca tgaaaacaag gcggtgcgaa
     6481 cgccgccaat gtgcaggtag ccggtggggc tgggggcgaa acgggttttg acggtcatga
     6541 tggctccaga atctttaaaa cggcttattt tactgttttt accgtgcttg ggcattaaaa
     6601 atgccgtccg aaccctgcct gcggataagt ttcagacggc attttccttg ttttcaatgc
     6661 ttcggcacgc ggaacagtgt atcacgcgcc gccgaccgga tttcttcggg attgcgtcca
     6721 aaaaaaagtt caatgaaaca gctaattgaa aaaatcccgc ccccattttt ccaaacggta
     6781 gagggataac gcatatccct cttgcagcat aaagattttt ttcttatttc ccgcatcaaa
     6841 ccgcgtggtc ggcgtggcag acatataaac gcggacaccc aaatcctccg ccatttccgc
     6901 cgcccgtgcc aaatggtagg gatcgctgac aatcaccacg ctggcaatac cgttggcacg
     6961 caaaaccgga cggatgttgt tcaggttttc ataagtgttg cgcgaagtgt tttcaaacag
     7021 gatgttgcgc gccggaaccc cctgtttgag cgcgtaccgc cgcccgacct cggcttcggt
     7081 catatagcct tttttggttc gccctcccgt aaacacgatt ttgcctaccc tgcggctctg
     7141 ataaagcgcg atggcgtggt tgatgcgttc ccgaaaaacc ggagaagggc gtttgtccca
     7201 cgcggcggca cccaacacca gcgcggcatc cgcccggaca tacggcggca aaacctgccc
     7261 gcccgtccga taaaccgccc aaacggatga ggcaaacacc agcaaaaacg gaaaaacact
     7321 caaacagaaa ccgcccaaca ggtaatagcg caagccgttg cggctgcaaa acagccgttt
     7381 gttcacaata ccgcttcgat attttccaac ggcctgccga cagccgcctt accgttcgcc
     7441 aaaacaatcg gacgctccaa cagggcggga tgatcggcga tggcacgcag cagcgcgtca
     7501 ttgtccaaat tggggttgtc caaacccaat tccttataca aatcatcttt cacgcgcatc
     7561 atcccgcgcg ccgatgccaa gcccaatttg ttgaaaatat ccttcaattc ggacaagtcg
     7621 ggcggcgtat ccaaatattt gaccacttcg gcagcaatgc cgcgttcttc caatagggac
     7681 aaggcggcac gcgatttgct gcaacggggg ttgtggaaaa ttttgatttc agacatgaca
     7741 tttccttact tctcgacaat ccccttatta tcggcttaca cagggtttta ctcaatatcc
     7801 cgcctacaac cgtaccaaac ggtttacaat acccgaatcg acatacaaag gacaaaacga
     7861 tgaaatactt gaatcttgcc gcaatcaccc ttgccgccac atttgccgca cataccgcct
     7921 cggcagacga actggccgga tggaaagaca acaccccgca aagcctgcaa tcactcaaag
     7981 cccccgtacg catcgtcaac ctttgggcaa cctggtgcgg cccgtgccga aaagagatgc
     8041 ctgccatgtc caaatggtac aaagcgcaga aaaaaggcag tgtcgatatg gtcggcatcg
     8101 cgctcgacac atccgacaat atcggcaact tcctcaagca aacccctgtt tcctacccga
     8161 tttggcgtta caccggggcg aacagccgaa actttatgaa atcctacgga aacaatgtcg
     8221 gcgtactgcc ctttaccgtc gtcgaagccc cgaaatgcgg atacaaacaa accattaccg
     8281 gggaagtgaa cgaaaaaagc ctgaccgaag ccgtcaaact cgcccattca aaatgccgtt
     8341 aaacgccgga tgccgtctga atccgcttca gacggcattt ttcccgcccg accttcggta
     8401 tccgccaaac ttatccacta tctaaaaaca ggcggaatct ttataatcgg cactgtctta
     8461 cctattgttc agacggcata tccctgcgga cgcaaccgcc cgaaacgata tgccgcccat
     8521 tccttacagg acctcctatg atccgtttcg aacaagtttc caaaacctat cccggcggtt
     8581 ttgaagccct gaaaaacgtc agcttccaaa tcaacaaagg cgaaatgata tttatcgcgg
     8641 gacactccgg ttcgggcaaa tccaccgtcc tcaagctgat ttcgggcatc accaagccga
     8701 gcaggggcaa aatcctgttt aacgggcagg acctcggcac attgtccgac aaccaaatcg
     8761 gctttatgcg ccaacacatc ggcatcgtgt tccaagacca caaaatcctc tacgaccgca
     8821 acgtcctgca aaacgtcatc ctgccgcttc ggattatcgg ctatccgccg cgcaaagccg
     8881 aagagcgtgc ccgcatcgcc atcgaaaaag tcggcctgaa aggacgagaa ttggacgatc
     8941 ccgtaaccct ctccggcggt gaacaacaac gcctgtgcat cgcccgcgcc gtcgttcacc
     9001 agcccggcct gctgattgcc gacgaaccct ccgccaacct cgaccgcgcc tacgcgctcg
     9061 atattatgga attgttcaaa accttccacg aagcgggaac aaccgtcatc gtcgccgcac
     9121 atgacgaaac cctgatggcg gactacggac accgcatcct gcgcctctcg aaaggacgac
     9181 tcgcatgagc atcatccact acctctcgct gcacgtcgaa tccgcgcgca ccgcgctcaa
     9241 acagcttctg cgccaacctt tcggcacact gcttaccctc ataatgctcg ccgtcgccat
     9301 gaccctgccg ctgtttatgt atttgggcat ccaaagcggg caaagcgtgt tgggcaaact
     9361 caatgagtcg ccgcaaatca caatctatat ggaaaccgcc gccgcacaaa gcgacagcga
     9421 taccgtccgc agcctgctgg cgcgcgacaa acggctcgac aacatccgct tcataggcaa
     9481 agaagacggt ctggaagaat tacagtccaa tctcgaccaa aatctgattt ccatgcttga
     9541 cggtaacccc ctgccggatg tctttatcgt tacccccgac ccggcaacca cgcccgccca
     9601 aatgcaggca atctaccggg acattaccaa actgcctatg gtcgaatccg ccaacatgga
     9661 tacggaatgg gtacagacac tgtaccaaat caacgaattc atccgtaaaa tcttatggtt
     9721 tctttccctg acgctgggga tggcgttcgt ccttgtcgcg cacaacacca tccgactgca
     9781 aatcctcagc cgcaaagaag aaatcgaaat caccaaactc ttgggcgcgc ccgcgtcgtt
     9841 tatccgccgc ccattccttt atcaagccat gtggcagagc atcctttccg ccgccgtcag
     9901 cttggggctt tgcggttggc tgctctctgc cgtgcgcccc ttggtcgatg ccatcttcaa
     9961 accctacgga cttaatatcg gctggcgttt cttctacccg ggagaaatcg cactggtgtt
    10021 cggcttcgtc atcgcgttgg gcgtattcgg cgcgtggctt gccaccaccc agcacctgct
    10081 cggcttcaaa gccaaaaaat aaaacaccgt caaaaatgcc gtccgaaccc gttttcagac
    10141 ggcatttcaa tttgccagta taatggcgca tttttccaac aaggaaccta ccatgctgac
    10201 ctcggaacaa gtaaaagcca tgattgaagg cgtggcaaaa tgcgaacata tcgaagtaga
    10261 aggcgacgga caccattttt tcgccgtcat cgtttcatca gaatttgaag gcaaggcacg
    10321 cctcgcgcgc caccgcctga ttaaagacgg actcaaagcc caactggaaa gtaacgaact
    10381 gcacgcactt tccatttcgg ttgccgccac tccggcggaa tgggcagcca aagcacaata
    10441 accgccacac aaaaatgccg tcagaaacag cacaacgttt cagacggcat tttttatatc
    10501 aaaccgctta cgcgccgcgt ttttccaaag cagctacggc aggcagctct ttgccttcca
    10561 agaactcaag gaacgcgcct ccgccggtag aaatatagcc gatttggtcg gtaacgccga
    10621 atttagcaat cgccgccagc gtgtcgccac cgcctgcaat cgagaatgct ttgctttggg
    10681 caatggcttc ggcaagggct ttcgtaccgc ctgcgaactg gtcaaactca aacacgccga
    10741 ccggcccgtt ccatacgacc gtgccggcgg ctttgagcaa ctccgcaagc gcggcggcgg
    10801 atttcgggcc gatgtccaaa atcatctcat cttcggcaac gtcggcaatg tctttcacga
    10861 ccgcttcggc atcggcagca aaggctttgg caacgacgac atcggtcggc agcggcacag
    10921 aaccgccttt ggccgccatt ttctccatga tttttttgga ttcttccacc aaatcgtgtt
    10981 ccgccaaaga tttgccgatg gctttacctt ccgccaacag gaaggtgttg gcgataccgc
    11041 cgccgacgat gagttggtcg actttgtccg ccagcgattc gaggatggtc agcttggtgg
    11101 acactttgct gccggcaacg atggcaacca tcgggcgcgc gggctgtttc aaggctttgc
    11161 ccaaagcgtc gagttcgccc gccatcaata cgccggcgca ggcaacggga gcggcttggg
    11221 cgacggcttc ggtcgaggct tgggcgcggt gcgcggtgcc gaacgcgtca ttgacgaaca
    11281 cgtcgcacaa agaagcgtag gctttgccca gttccaaatc gtttttcttc tcgcctttgt
    11341 tgatacgcac gttttgcagc atcacgacat cgcccgcgtt caaagccggt ttgttttcgc
    11401 gccagtcgtt caatactttc acgtttttgc ccaacaggcc gcccaaatgc gcggcaacgg
    11461 gcgcaacgtc gtcttcgggg tgaaactcgc cttcggtcgg gcggcccaag tgggtcatca
    11521 cgatgacgga cgcgccgttg tccacgcagt atttaacgga cgcaatcgag gcgcggatac
    11581 gggtgtcgtc gctgattttg ccgtctttga acggcacgtt catatcggcg cggatgagga
    11641 cggttttgcc ctgcacgttt tgttcggtca gttttaaaaa tgccataatc agtccttttc
    11701 aatcagtgtt tgcgatacag aaacaattaa tg
//
LOCUS       DAWHSK010000036        11169 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.036, whole genome shotgun sequence.
ACCESSION   DAWHSK010000036 DAWHSK010000000
VERSION     DAWHSK010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11169
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.036"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            188..622
                     /locus_tag="WIC03_000663"
     CDS             188..622
                     /locus_tag="WIC03_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEZ3883202.1"
                     /translation="MNFALSVITFTLASFLPVPPAEAAVFTWKDGGGNSYSDVPKQLH
                     PDQSQILNLRTRQTKPAIKPAQADAGKRTDGAAQENNPDTAEKNRQLEEEKKRIAETE
                     RQNKEENCRISKMNLKAVGNSNAKNKDDLIRKYNNAVNKYCR"
     gene            complement(671..2014)
                     /locus_tag="WIC03_000664"
     CDS             complement(671..2014)
                     /locus_tag="WIC03_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEZ3883203.1"
                     /translation="MFHSVNVFTGETLYRRPAQDYAEFERRLADLKMRGGAFAQLGVT
                     ERAARLQKFADRLEAEKERFAEMVCEEVGRCLHECRAEIGKSIELIRYYARLAPELLA
                     HKTIATQASLSQVRFEPLGVVFAVMPWNYPVWQVLRFAVPAMCAGNACAVKPAPSVAR
                     VSQALFDLVSDGIPLAGAWLDEAGTLKAVEDTDAMAFTGSTHTGRILAAHAGANLKKT
                     VLELGGSNAFIVMPDADLERAAAEACYSRFRDAGQSCNAAKRIIVTEAAADRFITLFL
                     AECAKLKMGDPKHPDTTLAPLHREDLRDRVHGQVEDAVSNGAVCLTGGKIPQGRGWFY
                     PATVLDRVNPACRVWREEVFGPAALILRAENEEHAIALANDSPFGLGACIYTADTERA
                     WRFAEKIQAGSVFINRHTSSDLRLPFGGVKDSGYGRELSEFGLYEFVNVKTYWQK"
     gene            complement(2019..2672)
                     /locus_tag="WIC03_000665"
     CDS             complement(2019..2672)
                     /locus_tag="WIC03_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEZ3883204.1"
                     /translation="MGLGMEIGKLIVAFLVLINPFSALSLYLDLTNGHSTKERRKVAR
                     TAAVAVFAVIAVFALIGGTLLKVLGISVGSFQVGGGILVLLIAISMMNGNDNPAKQNL
                     GAQPETGQARPARNAGAIAVVPIAIPITIGPGGISTVIIYASAAKTYSDIALIIVAGL
                     VVSAICYAILIVAGKVSRLLGATGLTILNRIMGMMLAAVSVEIIVSGLKTIFPQLAG"
     gene            2823..3113
                     /gene="gatC"
                     /locus_tag="WIC03_000666"
     CDS             2823..3113
                     /gene="gatC"
                     /locus_tag="WIC03_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEZ3883205.1"
                     /translation="MALTLADVDKIARLSRLHLTAEEKEKSLQELNDIFTMVEQMQTI
                     NTDGIEPMAHPHEAALRLREDEVTETDRAAEYQAVAPEVRDRLYIVPQVIEE"
     gene            3176..4621
                     /gene="gatA"
                     /locus_tag="WIC03_000667"
     CDS             3176..4621
                     /gene="gatA"
                     /locus_tag="WIC03_000667"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEZ3883206.1"
                     /translation="MTQYTLKQAGSLLQSKQISAVELASAYLAAIAEKNPALNGYITI
                     DQDKTLAEARAADERIAQGNASALTGVPVAYKDIFCQTGWRSACASKMLDNFISPYTA
                     TVVQNLLDEGMVTLGRTNMDEFAMGSTNENSFYGAAKNPWNPEHVPGGSSGGSAAVVA
                     ARLAPAALGSDTGGSIRQPASHCGITGIKPTYGTVSRFGMVAYASSFDQAGPMAQTAE
                     DCAILLNAMAGFDPKDSTSLEREKEDYTRDLNQPLKGVKIGLPKEYFGEGNSADVQTA
                     LQNTINLLKTQGAELVEVSLPQTKLSIPAYYVLASAEASTNLSRYDGVRYGHRAAQFG
                     DLEEMYGKTRAEGFGSEVKRRIMIGTYVLSHGYYDAYYLKAQKLRRLVADDFQTAFAR
                     CDLILAPTAPTAAPKIGADASPVETYLSDIYTIAVNLAGLPALTLPAGFSGGGLPVGV
                     QLVGNYFAEAKILGAAHQIQLNSDWHGKRPE"
     gene            4618..5550
                     /locus_tag="WIC03_000668"
     CDS             4618..5550
                     /locus_tag="WIC03_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HEZ3883207.1"
                     /translation="MKQNRTFTFPDFRTVYSYAPLYRLQHLKYTLRKFFGKKEIYAFE
                     QFVNASPIRQGLFLHCPQNAYPLLREFVDRRFNCKRRLDAMTADFLMAEKLFGTDILH
                     QMEDYRFHLVLAHLSDGISLWLNRNDNCVEEGAWSLSLRDEAGNRLYMATFAFVGTHL
                     LTASVQGPAGEEAKDTVRRITKQLHGLRPQQLMVTALQYFAAVLGLDGAMGIAQKHQV
                     KLRWKLKKRVKMNYDAFWQEYGASLERDGYWHLPQTPARKDLTDIESKKRSMYRKRYE
                     MLDNMVAEMKDGLKTEARGISDGIQTEKPLRRTA"
     gene            5593..7023
                     /gene="gatB"
                     /locus_tag="WIC03_000669"
     CDS             5593..7023
                     /gene="gatB"
                     /locus_tag="WIC03_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEZ3883208.1"
                     /translation="MTWQTVIGLEIHVQLNTKSKIFSGASTAFGAEPNAHAGVVECAL
                     PGVLPVMNREVVEKAIKLGLALDAKINRKNVFDRKNYFYPDLPKGYQISQLDLPIVEH
                     GKLEIVVGDDVKTINVTRAHMEEDAGKSVHEGLNGATGIDLNRAGTPLLEVVSEPEMR
                     SAAEAVAYAKALHSLVTWLNICDGNMAEGSFRVDANVSVRPKGQAEFGTRREIKNLNS
                     FRFLEQAINYEAEAQIEILEDGGKVQQATMLFDPEKGETRVMRLKEDAQDYRYFPDPD
                     LLPVIISDAQMQKAKAEMPELPKEMAARFVADYGVSEYDARLLTASRAQAAYFEEAAK
                     ESGQGKPTANWMNGELAAALNKEGMELADSPITAPRLAALVGKIADGTLSSKLAKKAF
                     EAMWAESEATIAEIIEKHGLQQMTDTGAIEAMVDEVLANNAKAVEQFKSGNEKALNAI
                     VGQVMKASKGKANPAQVQELIKAKLA"
     gene            7115..8125
                     /locus_tag="WIC03_000670"
     CDS             7115..8125
                     /locus_tag="WIC03_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HEZ3883209.1"
                     /translation="MNHTVTLPDQTTFAANDGETVLAAAARQNLNLPHSCKSGVCGQC
                     KAELVSGDIQMDGHSEQALSEAEKAQGKILMCRTTAQSDISINIPGYKADALPVRTLP
                     ARIESIIFKHDVALLKLALPKAPPFAFYAGQYIDLLLPGNVSRSYSIANPPDQEGILE
                     LHIRRRKNGVCSEMIFGSEPKVKEKGIVRVKGPLGSFTLQEDSGKPVILLATGTGYAP
                     IRSILLDLIRQNSSRAVHFYWGARHQDDLYALEEAQGLACRLKNACFTPVLSRPGEGW
                     QGRKGHVQDIAAQDHPDLSEYEVFACGSPAMTEQAKNLFVQQHKLPENLFFSDAFTPS
                     AS"
     gene            complement(8254..8886)
                     /gene="pdxH"
                     /locus_tag="WIC03_000671"
     CDS             complement(8254..8886)
                     /gene="pdxH"
                     /locus_tag="WIC03_000671"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEZ3883210.1"
                     /translation="MDLHNIREDYSKRELSEGDCADNPIEQFERWLDEAVRAQVNEPT
                     AVNVAAVDGRGRPNSRMVLLKEVNSEGFVFFTNYHSRKGRSLDAHPFAAMTFFWPELE
                     RQVRVEGRVERLAEKLSDEYFESRPYQSRLGAWASAQSEVIPNKAVLVAKAAAVGLKH
                     PLHVPRPPHWGGYIVIPDLIEFWQGRPSRLHDRIQYRLLDGGWIRERLSP"
     gene            9117..10166
                     /locus_tag="WIC03_000672"
     CDS             9117..10166
                     /locus_tag="WIC03_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEZ3883211.1"
                     /translation="MSKQPTSKRQWRDGVAPSAKKTAKPFKSKARPKDETDKTASQPY
                     GQKASDGIKPQNLPKQRAAKAKKLVVRNPNQKIMEHARDLKERRSDLSRMEPERLQKV
                     LAASGVGSRREMEEWINNGWVTVNGKTAQLGDKVTPDDHVTVKGSIIKLKWADRLPRI
                     ILYYKQEGEIVSRDDPQGRVSIFDRLPQAASSRWVAIGRLDINTSGLLILTTSGELVQ
                     RFAHPSFEVEREYAVRVLGGLTGEQMRVLTEEGVMLEDGLAKVERIREQGGEGANKWY
                     NVVIKEGRNREVRRIFESQGLTVSRLVRVGFGPIGLPNRLKRGQFYELNPAEVANILK
                     WAEMLLPGERRRKKA"
     gene            complement(10235..>11169)
                     /locus_tag="WIC03_000673"
     CDS             complement(10235..>11169)
                     /locus_tag="WIC03_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ3883212.1"
                     /translation="MFGAFTIRVPADGWKPEGYTAPKTQNKLVSSNHVNVSQAMKTPQ
                     FWLLFWVLCLNVTAGIGVLGQASVMIQELFSETSVGRQASVGAGAAAGFVSLLSLFNM
                     GGRFLWSSVSDKIGRKNTYTIFFVLGSLLYFAVPSIGEGGSKALFIIGFCVIISMYGG
                     GFAAIPAYLKDLFGTYQVGAIHGRILLAWSTAAVIGPVLVNYIRQSQIDSGIPAAQAY
                     SVTMYIMACLLIIGLLCNLAVKSVHEKHHEKDIKTAAHSGNPDDETAISDAYLVGEKV
                     SGGGISVWWRWALAVIPLAYGVVMVFVKALDLFS"
BASE COUNT         2876 a         3441 c         2740 g         2112 t
ORIGIN      
        1 tctgcggctt cgtcgccttg tcctgatttt tgttaatcca ctatattttt gggaatctgt
       61 tttaccccaa tatataaagc accatattaa ggcggagttt cttccccact ttgaaccgaa
      121 cccggaaaag acaccgccca agccaatcct gatgctgccc cggcagccaa ccatcaagga
      181 aaccctaatg aactttgctt tatccgtcat cacatttacc ctcgcctctt tcctgcccgt
      241 cccgcccgcc gaagccgccg tctttacttg gaaggacggc ggcggcaaca gctattcgga
      301 tgtgccgaaa cagcttcatc ccgaccagag ccaaatcctc aacctgcgga cgcgccaaac
      361 caaaccggcg atcaaacccg cccaagccga cgcagggaag cgcacagacg gcgcggcaca
      421 ggaaaacaat cccgacactg ccgagaaaaa ccggcagctt gaggaagaaa agaaaagaat
      481 tgccgaaacc gaacggcaga acaaagaaga aaactgccgg atttcaaaaa tgaacctgaa
      541 agcggtggga aattcaaatg caaaaaacaa ggatgatttg attcggaaat acaataacgc
      601 cgtaaacaaa tactgccgtt aatcggctct agcgcaaacc ccatgccgtc tgaaacggca
      661 cggggtttgt ttatttctgc cagtaggttt tgacgttgac gaactcgtac agcccgaatt
      721 cggacaattc gcgcccgtaa ccggaatctt tgactccgcc gaaaggcagg cgcaaatcgc
      781 tgctggtatg gcggttgata aacaccgatc ccgcctgtat tttttcggca aaccgccaag
      841 cgcgttcggt atcggcggta taaatgcagg cacctagccc gaacggggaa tcattggcaa
      901 gggcgatggc atgttcttcg ttttcggcgc gcaaaatcag ggcggccggc ccgaatactt
      961 cttctctcca gacgcggcag gcaggattta ccctgtctaa aaccgtcgcg ggataaaacc
     1021 agcctcgccc ttgtgggatt tttccgccgg tcaggcatac cgcgccgttt gaaacggcat
     1081 cttcaacctg cccgtgaacc ctgtcccgca aatcttcgcg gtgcagcggt gcaagcgtag
     1141 tatcgggatg tttggggtcg cccattttca atttggcgca ttcggcaaga aacagcgtga
     1201 taaaacgatc ggctgcggct tcggttacga tgatgcgttt ggcggcgttg cacgattgcc
     1261 ccgcatcgcg gaaacgggaa taacaggctt ctgcggcggc gcgttccaaa tcggcatcgg
     1321 gcatcacgat aaaggcgttg ctgccgccga gttccaagac ggttttcttg aggtttgcgc
     1381 ccgcatgtgc cgcaaggatg cgccccgtat gcgtcgagcc ggtaaacgcc atcgcatcgg
     1441 tatcttcaac cgccttgagc gtgcccgcct catccaacca cgcgcctgcc aaaggaatgc
     1501 cgtctgaaac caaatcaaac aacgcctgac tgacgcgtgc cacgctgggc gcgggtttga
     1561 cggcgcacgc gttgcccgcg cacatagcgg gaacggcgaa acgcaatacc tgccagacgg
     1621 gatagttcca aggcatgacg gcaaacacca cgcccaaagg ctcaaacctt acctgactca
     1681 aactcgcctg cgtcgcgatg gttttgtggg cgagcagttc gggagcgagg cgggcgtaat
     1741 agcgtatcag ttcgatagac ttgccgattt ccgcacggca ttcgtgcagg cagcgtccga
     1801 cttcttcgca caccatttcc gcaaaacgct ctttctccgc ctccaaacgg tcggcaaatt
     1861 tttgcagacg cgcggcacgt tcggttacgc ccagttgcgc gaacgccccg ccgcgcattt
     1921 tcaaatccgc cagccgccgt tcaaactccg cataatcttg agctggacgg cggtaaagcg
     1981 tttcgcccgt aaatacattg acactgtgaa acatcgaatc aacctgccag ttgcgggaat
     2041 atcgttttca gtcccgacac aataatctcc accgataccg ccgccagcat catacccatg
     2101 atacggttta aaatcgtcag ccccgtcgcg cccagcaggc ggctgacctt ccctgcaaca
     2161 attaaaatgg cataacaaat cgcactgacc accaaaccgg ccacgataat cagcgcgatg
     2221 tcgctgtacg ttttggctgc cgaagcgtaa ataatcacgg tcgaaatacc gcccgggccg
     2281 atggtgatcg gtatggcgat gggcacgacg gcaatcgctc cggcattgcg ggcggggcgc
     2341 gcctgccccg tttccggctg cgcgccgaga ttctgcttgg cgggattgtc gttgccgttc
     2401 atcatcgaaa tggcgatcag cagcaccaaa atcccgccgc cgacctgaaa cgaaccgacg
     2461 ctgatgccca aaaccttcag cagcgtaccg ccgatcagcg caaataccgc aatcacggca
     2521 aacacggcaa cggcggccgt ccgcgcgacc ttcctgcgct ccttcgtgct gtgcccgttg
     2581 gtcaggtcaa ggtaaagcga caacgcgcta aacggattaa tcagcaccaa aaaagccaca
     2641 atcagcttgc cgatttccat gcccaatccc attatttccc cctccttcaa acccgtgcgg
     2701 caggcatccg atgctgcaaa ttgccgccgc aacggatttt tccgttataa ttaaaaattc
     2761 aagcaatacg ccccatcata cccgaacgac ggtatcttta ccatcagaca aggatgcttt
     2821 tcatggcact gacacttgcc gacgtagaca aaatcgcccg actctcccga ctgcacctga
     2881 ctgcggaaga aaaagaaaaa tcgcttcaag aattaaacga cattttcact atggtcgaac
     2941 agatgcaaac cattaacaca gacggcatcg aaccgatggc gcacccgcac gaggccgccc
     3001 tgcgcctgcg cgaagacgaa gtaaccgaaa ccgaccgcgc cgccgaatat caggcggtcg
     3061 caccggaagt acgcgaccgc ctgtacatcg taccgcaagt tatcgaagaa taatccgaat
     3121 acgcttcaga cggcatcagc aataccgccc gaagcccttt aaggatggaa gatttatgac
     3181 ccaatacaca ttgaaacagg cgggcagcct gttgcagtcc aaacagattt ccgccgtcga
     3241 actggcaagc gcataccttg ccgccatcgc cgaaaaaaat cccgccctca acggctatat
     3301 caccatcgac caagataaaa cccttgcaga agcccgtgcc gccgacgaac gtatcgcgca
     3361 gggcaacgcc tccgcgctta ccggcgtacc cgtcgcctac aaggatattt tctgccaaac
     3421 cggctggcgc agcgcgtgcg cttccaaaat gctcgacaac ttcatctccc cctacaccgc
     3481 caccgtcgtc caaaacctgc tcgacgaagg tatggtaacg ctcggccgca ccaatatgga
     3541 tgagttcgct atgggttcga ccaatgaaaa ctcattctac ggcgcggcca aaaatccgtg
     3601 gaatccggaa cacgtccccg gcggttcgtc aggcggttcc gccgccgtcg ttgccgctcg
     3661 ccttgcccct gccgcgctcg gttcggatac cggcggctct atccgccagc ctgcatcgca
     3721 ctgcggcatt accggcatca aacccacata cggcacggtt tcccgcttcg gtatggtcgc
     3781 ctacgcctcc agcttcgacc aggccggtcc gatggcgcaa accgccgaag actgcgcgat
     3841 tctgttgaac gcgatggcag gtttcgaccc caaagactcc accagcctcg aacgcgaaaa
     3901 agaagactac acccgcgatt tgaaccaacc gctcaaaggc gtgaaaatcg gcctgcccaa
     3961 agaatatttc ggcgaaggca acagcgccga tgttcagacg gcattgcaaa atactatcaa
     4021 cctcctcaaa acccaaggcg cggaattggt cgaagtttcc ctgccgcaaa ccaagctgtc
     4081 catccccgcc tactacgtcc tcgcctccgc agaagccagt accaaccttt cacgttacga
     4141 cggcgtacgt tacggacacc gtgccgccca attcggcgat ttggaagaaa tgtacggcaa
     4201 aacccgcgcc gaaggtttcg gcagcgaagt taaacgccgc atcatgatcg gcacttatgt
     4261 actgtcacac ggctactacg atgcctacta tctcaaagcc caaaaactgc gccgcctcgt
     4321 tgccgatgat tttcagacgg catttgcacg gtgcgacctc atcctcgcgc cgaccgcacc
     4381 cactgcagcc ccaaaaatcg gagcggatgc ttcgccggtt gaaacctact tgagcgatat
     4441 ctacaccatc gccgtcaacc tcgccggact gcccgcattg accctgcccg caggcttcag
     4501 cggcggcgga ctgcccgtcg gcgttcagct tgtcggcaac tacttcgccg aagccaaaat
     4561 cctcggtgcg gcgcatcaaa tccaactcaa cagcgattgg cacggcaaac gacccgaatg
     4621 aagcagaacc gcacctttac cttccccgat tttcgcaccg tttacagcta tgcgccttta
     4681 tatcggctgc aacatttaaa atacacattg cgaaaatttt tcggaaaaaa agaaatttac
     4741 gccttcgagc agtttgtcaa cgcatcccct atccgtcagg ggctgttcct ccactgcccg
     4801 caaaatgcct atccgctgct gcgcgaattt gttgacaggc gttttaactg caaacgccgt
     4861 ttagatgcga tgacggcaga ttttctcatg gcggaaaaac tgttcggcac agacatcctg
     4921 caccaaatgg aagactaccg cttccatttg gtcttggcgc acctttcaga cggcatcagc
     4981 ttgtggctca accgcaacga caactgcgtc gaagaaggcg cgtggtcttt atctttgcgc
     5041 gacgaagcag gcaaccggct gtatatggcg accttcgcct ttgtcggcac acacctgctg
     5101 acagcctccg tacaagggcc ggcgggtgaa gaagccaaag acaccgtccg ccgcataacc
     5161 aaacaactcc acggcttgcg tccccaacaa ctgatggtaa ccgccctgca atatttcgcc
     5221 gccgtactcg gcttggacgg cgcaatgggc attgcacaaa aacatcaggt caaactgcgc
     5281 tggaaactta aaaagcgtgt caaaatgaat tacgacgcat tctggcagga atacggcgca
     5341 agtttggaac gggacggcta ctggcatctc ccccaaaccc ccgcccgcaa agaccttacc
     5401 gacatcgaaa gcaaaaaacg ctcgatgtac cgcaagcgtt atgaaatgct ggacaatatg
     5461 gttgcagaga tgaaggacgg tctgaaaaca gaagcacgcg gcatttcaga cggcatccaa
     5521 acggaaaaac cgctccgccg gacagcctga cgcgaagact atcgaattga tattttagag
     5581 aaagaagctc ttatgacctg gcaaaccgta atcggcttgg aaatccacgt ccaattgaac
     5641 accaaatcca aaatcttcag cggcgcatcg accgcattcg gcgcagaacc caacgcgcac
     5701 gccggcgtag tggaatgtgc tttgccgggc gtactgccgg taatgaaccg agaagtcgtt
     5761 gaaaaagcca tcaaattagg cttggctttg gatgcgaaaa tcaaccggaa aaacgtgttc
     5821 gaccgtaaaa actacttcta tcccgactta ccaaaaggct atcaaatcag ccagttggac
     5881 ttaccgattg tcgaacacgg caaattggaa atcgtagtcg gcgacgatgt gaaaaccatc
     5941 aacgtaaccc gcgcccatat ggaagaagac gcgggcaagt ccgtacatga aggcttgaac
     6001 ggcgcgaccg gtatcgattt gaaccgcgcc ggcacgcctt tattggaagt cgtatccgaa
     6061 cccgaaatgc gctccgccgc cgaagccgtt gcctacgcca aagccttgca cagcttggta
     6121 acctggctga acatttgcga cggcaatatg gcggaaggtt cgttccgcgt cgatgccaac
     6181 gtatccgttc gcccgaaagg tcaagcggaa ttcggcacgc gccgcgagat taaaaacctc
     6241 aattccttcc gtttcttgga gcaggcgatt aattacgaag cagaagcgca aatcgagatt
     6301 ttggaagacg gcggcaaagt acagcaggca acgatgctgt ttgatcccga aaaaggcgaa
     6361 acccgcgtaa tgcgcctgaa agaagacgcg caagactacc gctacttccc cgaccccgat
     6421 ttgctgcccg tcatcatttc agatgcccaa atgcaaaaag ccaaagcaga aatgcccgag
     6481 ctgccgaaag aaatggcagc gcgtttcgtg gcggactacg gcgtgtccga atacgacgcg
     6541 cgcctgctca ccgcaagccg cgcacaggcc gcctattttg aagaagccgc caaagagagc
     6601 ggacaaggca agccgactgc caactggatg aacggcgaac tcgccgccgc actgaacaaa
     6661 gaaggcatgg aacttgccga cagcccgatt accgctccgc gcctcgccgc gctggtaggc
     6721 aaaatcgccg acggcacatt aagcagcaaa ttagcgaaaa aagcctttga agccatgtgg
     6781 gcagaatccg aagccaccat tgccgaaatc attgaaaaac acggtttgca gcaaatgacc
     6841 gataccggcg cgattgaagc catggtggac gaagtgctgg caaacaacgc caaagccgtg
     6901 gaacagttca aatccggcaa cgaaaaagcc ctgaatgcga ttgtgggaca agtgatgaag
     6961 gccagcaaag gcaaagccaa ccccgcgcag gttcaagagc tgattaaagc caaacttgcc
     7021 tgacaagcaa gatgccgtct gaaacccaaa tccgacgttc agacggcatt ttcacagtct
     7081 gaccacatta aaacagcata aaaaacaata ttccatgaac cacaccgtta ccctgcccga
     7141 ccaaaccacc tttgccgcca acgacggcga aaccgttttg gccgctgccg cccgacaaaa
     7201 cctcaacctg ccccattcct gcaaaagcgg tgtctgcgga caatgcaaag ccgaactggt
     7261 cagcggcgat attcaaatgg acggacactc ggaacaggct ttatccgaag cagaaaaagc
     7321 gcaaggcaag attttgatgt gccgcaccac cgcgcaaagc gatatcagca tcaacatccc
     7381 cggctacaaa gccgatgccc tacccgtccg caccctgccc gcacgcatcg aaagtattat
     7441 tttcaaacac gatgtcgccc tcctgaaact tgccctgccc aaagccccgc cgtttgcctt
     7501 ctacgccggg caatacattg atttactgct gccgggcaac gtcagccgca gctactccat
     7561 cgccaatccg cccgaccaag aaggcatttt ggaactacac atccgcaggc gcaaaaacgg
     7621 tgtctgctcg gaaatgattt tcggcagcga acccaaagtc aaagaaaaag gcatcgtccg
     7681 cgttaaaggt ccgctcggtt cgtttaccct gcaggaagat agcggcaaac ccgtcatcct
     7741 gctggcaacc ggcacaggct acgcccccat ccgcagcatc ctgctcgacc ttatccgcca
     7801 aaacagcagc cgcgccgtcc atttctactg gggcgcgcgt catcaagatg atttgtatgc
     7861 cctcgaagaa gcacaagggt tggcatgccg tctgaaaaac gcctgcttca cccccgtatt
     7921 gtcccgcccc ggagagggct ggcagggaag aaaagggcac gtacaagaca tcgcggcaca
     7981 agaccacccc gacctgtcgg aatacgaagt atttgcctgc ggctctccgg ccatgaccga
     8041 acaagcaaaa aatctgtttg tgcaacagca taagctgccg gaaaacttgt ttttctccga
     8101 cgcattcacg ccgtccgcat cataattccc cggtataaaa aagattcagg ctttccgttc
     8161 ggaacacaaa aaacttcccg tccgtatttt ccccataaaa aatgccgtct gaagcccgat
     8221 tccggttttc agacggcata tgttttttcc tgttcaaggc gacagccgct cgcgtatcca
     8281 gccaccatcc agcaaacggt attggatgcg gtcgtgcagc ctgctcggcc tgccctgcca
     8341 aaactcaatc aaatcgggaa tcacaatata gccgccccaa tgcggcggac gcggcacgtg
     8401 caaaggatgt ttgagtccga ccgccgccgc ctttgccacc aataccgcct tgttcggaat
     8461 cacctcgctc tgcgcacttg cccacgcacc caaacggctc tgatacgggc ggctctcaaa
     8521 atattcgtcc gacaacttct ccgccagcct ttcaacacgc ccttccacgc gcacctgacg
     8581 ctccagctcc ggccaaaaaa acgtcatcgc cgcaaatgga tgagcatcca gcgaacgtcc
     8641 cttacggctg tgataattcg taaagaaaac aaaaccttca gaattaactt ccttcagcag
     8701 caccatacgg ctgttgggtc tgccgcgtcc gtcgaccgcc gccacattaa ccgccgtcgg
     8761 ctcgttgacc tgtgcgcgta ccgcctcgtc caaccaccgc tcgaactgct cgatcggatt
     8821 gtcggcgcaa tcgccttccg acaattcccg tttactgtaa tcttcccgaa tattgtgcaa
     8881 atccatttac cgctcctctt gtcatttgaa agattctacc cccgcacgca aaccgatttc
     8941 aaccgttgca caaactttgc ctcgcttctc caaacctcag gacacaacga tttcatccgc
     9001 aaaaccgccg cataaggtac aatatcgaac cgtccgaccg agggcggcat tttatcaacc
     9061 cgtcctgccg cacacgccgc agaagaaccg ccttatcagg cgagttagga aaaatgatgt
     9121 ccaaacagcc caccagcaaa cgccaatggc gcgacggcgt agccccgtct gccaagaaaa
     9181 ccgccaaacc tttcaaaagc aaagcccgtc ccaaagacga aacggacaaa accgcatccc
     9241 aaccttacgg acaaaaagct tcagacggca tcaaacctca aaacctcccc aaacagcgcg
     9301 ccgccaaagc caaaaaactc gtcgtccgca atcccaacca aaaaattatg gaacacgcgc
     9361 gcgatttaaa agaacgccgc agcgacctat cgcgcatgga acccgaacgc ctgcaaaaag
     9421 tgcttgccgc gtccggcgtc ggctcgcgcc gcgaaatgga agaatggatc aacaacggct
     9481 gggtaacggt caacggcaaa accgcgcaac tgggcgacaa agtcaccccc gacgaccacg
     9541 ttaccgtcaa aggcagcatc atcaaactca aatgggccga ccgcctgccg cgcatcatcc
     9601 tatattacaa acaagaaggc gaaatcgttt cccgcgacga cccgcaaggc cgcgtcagca
     9661 tattcgaccg cctgccgcag gccgccagca gccgctgggt cgccatcgga cgcttggaca
     9721 tcaacaccag cggacttctg attctcacca cctccggcga actcgtccaa cgtttcgccc
     9781 accccagctt cgaagtcgaa cgcgaatacg ccgtgcgcgt actgggcggg ctgaccggcg
     9841 aacaaatgcg cgtcctcacc gaagaaggcg tgatgctcga agacggcttg gcaaaagtcg
     9901 aacgcatccg cgaacaaggc ggcgaaggcg cgaacaaatg gtacaacgtc gtgattaaag
     9961 aaggccgcaa ccgcgaagtg cgccgcattt ttgaaagcca aggactcacc gtcagccgcc
    10021 tcgtgcgcgt cggcttcggc cccatcggac tgcccaaccg cctcaaacgc ggacagttct
    10081 acgaactcaa ccccgccgaa gtcgccaaca tcctcaaatg ggcggaaatg ctgctgccgg
    10141 gcgaacgccg ccgcaaaaaa gcctaaaccc gccaaaacac aaaaatgccg tctgaaacat
    10201 ctactgtttc agacggcatt ttattcgggc gttttcagga gaaaaggtcg agtgctttga
    10261 caaagaccat caccacgccg taggcgagcg gtatgacggc caatgcccaa cgccaccaga
    10321 ccgatatgcc gccgccggaa actttttcgc ccacaaggta agcgtcggat atggcggttt
    10381 cgtcatcggg gttgccgctg tgtgcggcgg ttttgatgtc tttttcgtgg tgtttttcgt
    10441 gtacggattt gacggcgagg ttgcacaaca aaccgataat cagcaggcac gccatgatgt
    10501 acatggttac gctgtatgcc tgtgccgccg gtatgccgct gtcgatttgg ctttggcgta
    10561 tgtaattgac cagtaccggg ccgatgacgg cggcggttga ccaggccagc aggatgcgcc
    10621 cgtgaatcgc gccgacctga taggtgccga acaggtcttt caggtaggcg ggaatggcgg
    10681 caaatccgcc gccgtacatg gaaataatca cgcaaaagcc gatgatgaac agggctttgc
    10741 tgccgccctc gccgatggag ggaacggcga aatacagcag cgaaccgagt acgaagaaaa
    10801 tggtgtaggt gtttttgcgt ccgattttgt cggaaacgct cgaccataaa aagcgtccgc
    10861 ccatgttaaa caggctcagg aggctgacga agcctgccgc cgcacctgcg ccgactgacg
    10921 cctgcctgcc tacggaggtt tcggaaaaga gttcctgaat catcacggat gcctgaccca
    10981 atacgccaat gccggcggtt acgttcaggc acaataccca gaacaacagc caaaattgcg
    11041 gcgttttcat ggcttgtgac acgttgacat gattgctgct gaccagcttg ttttgcgttt
    11101 tcggcgcggt atagccttca ggtttccagc cgtcggcagg tacgcggatg gtaaacgcgc
    11161 cgaacatca
//
LOCUS       DAWHSK010000037        11159 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.037, whole genome shotgun sequence.
ACCESSION   DAWHSK010000037 DAWHSK010000000
VERSION     DAWHSK010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11159
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.037"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(107..934)
                     /gene="fghA"
                     /locus_tag="WIC03_000674"
     CDS             complement(107..934)
                     /gene="fghA"
                     /locus_tag="WIC03_000674"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011961786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HEZ3883213.1"
                     /translation="MKLIEQHQIFGGSQQVWAHHAQTLQCEMKFAVYLPNNPENRPLG
                     VIYWLSGLTCTEQNFITKSGFQRYAAEHQVIVVASDTSPRGEQVPNDDAYDLGQGAGF
                     YLNATEQPWAANYQMYDYILNELPRLIEKHFPTNGKRSIMGHSMGGHGALVLALRNRE
                     HYQSVSAFSPILSPSLVPWGEKAFTAYLGKDREKWQQYDANSLIQQGYKVQGMRIDQG
                     LEDEFLPTQLRTEDFIETCRAANQPVDVRFHKGYDHSYYFIASFIGEHIAYHAAFLK"
     gene            complement(943..2052)
                     /locus_tag="WIC03_000675"
     CDS             complement(943..2052)
                     /locus_tag="WIC03_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HEZ3883214.1"
                     /translation="MKSRAAVAFAPNQPLQIVEIDVEMPRKGEVLIRNTHTGVCHTDA
                     FTLSGSDPEGVFPVVLGHEGAGVVVAVGEGVSSVKPGDHVIPLYTAECGECEFCCSGK
                     TNLCVSVRDTQGKGLMPDGTTRFSYQGQPIYHYMGCSTFSEYSVVAEVSLAKINPEAN
                     HEQVCLLGCGVTTGIGAVHNTAKVQEGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPAKFELAKQFGATDCLNPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRQALES
                     AHRGWGQSIIIGVAGAGQEISTRPFQLVTGRVWKGSAFGGVKGRSELPKMVEDSMKGD
                     IELEPFVTHTMTLDQINKAFELMHEGKSIRAVIHY"
     gene            2200..2607
                     /locus_tag="WIC03_000676"
     CDS             2200..2607
                     /locus_tag="WIC03_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEZ3883215.1"
                     /translation="MTYTTAKAAEKIGISAHTLRFYDKEGLLPNIGRDEYGNRCFTDN
                     DLQWLGLLQCLKNTGMSLKDIKRFAECTVIGDDTIEERLSLFENQIENVKCQIAELKR
                     YLDLLEYKLAFYQKAKALGSVKAVNLPQIPETA"
     gene            complement(2958..3272)
                     /locus_tag="WIC03_000677"
     CDS             complement(2958..3272)
                     /locus_tag="WIC03_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HEZ3883216.1"
                     /translation="MTKSELMVRLAEVFAAKNGTHLLAKDVEYSVKVLVDTMTRSLAR
                     GQRIEIRGFGSFDLNHRPARIGRNPKTGERVEVPEKHVPHFKPGKELRERVDLALKEN
                     AN"
     gene            complement(3283..4968)
                     /gene="rpsA"
                     /locus_tag="WIC03_000678"
     CDS             complement(3283..4968)
                     /gene="rpsA"
                     /locus_tag="WIC03_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEZ3883217.1"
                     /translation="MSMENFAQLLEESFTLQEMNPGEVITAEVVAIDQNFVTVNAGLK
                     SESLIDVAEFKNAQGEIEVKVGDFVTVTIESVENGFGETKLSREKAKRAADWIALEEA
                     MENGNILSGIINGKVKGGLTVMISSIRAFLPGSLVDVRPVKDTSHFEGKEIEFKVIKL
                     DKKRNNVVVSRRAVLEATLGEERKALLENLQEGSVIKGIVKNITDYGAFVDLGGIDGL
                     LHITDLAWRRVKHPSEVLEVGQEVEAKVLKFDQEKQRVSLGMKQLGEDPWSGLTRRYP
                     QGTRLFGKVSNLTDYGAFVEIEQGIEGLVHVSEMDWTNKNVHPSKVVQLGDEVEVMIL
                     EIDEGRRRISLGMKQCQANPWEEFAANHNKGDKISGAVKSITDFGVFVGLPGGIDGLV
                     HLSDLSWTESGEEAVRKYKKGEEVEAVVLAIDVEKERISLGIKQLEGDPFGNFISVND
                     KGSLVKGSVKSVDAKGAVIALSDEVEGYLPASEFAADRVEDLTTKLKEGDEVEAVIVT
                     VDRKNRSIKLSVKAKDAKESREALNSVNAAANANAGTTSLGDLLKAKLSGEQE"
     gene            complement(5123..5779)
                     /locus_tag="WIC03_000679"
     CDS             complement(5123..5779)
                     /locus_tag="WIC03_000679"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEZ3883218.1"
                     /translation="MNRQKVIAIDGPGASGKGTVAARVAAALGYDYLDTGALYRLTAL
                     YAQKQGVEWHDEENVSKLAKKLPAVFSGSRILLDGEDVSDGIRTEAIGMGASAVAQLP
                     KVRAALLQRQRDFLTEKGLVADGRDTGSVVFPQAELKIFLTAESKIRAERRAKQIGIP
                     CEGLAFERILSDIEARDEADRNRKVAPLKQQPDALLLDTSRLTIEETVKKVLDWYRKV
                     "
     gene            complement(6023..6831)
                     /locus_tag="WIC03_000680"
                     /pseudo
     CDS             complement(6023..6831)
                     /locus_tag="WIC03_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232575.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
     gene            complement(6891..7333)
                     /locus_tag="WIC03_000681"
                     /pseudo
     CDS             complement(6891..7333)
                     /locus_tag="WIC03_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250212.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
     gene            7659..7765
                     /locus_tag="WIC03_000682"
                     /pseudo
     CDS             7659..7765
                     /locus_tag="WIC03_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(7967..8659)
                     /locus_tag="WIC03_000683"
     CDS             complement(7967..8659)
                     /locus_tag="WIC03_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEZ3883219.1"
                     /translation="MKLIKYLQYQGIGSRKQCQWLIAGGYVFINGTCMDDTDADIDSS
                     SVETLDIDGEAVTVVPEPYFYIMLNKPEDYETSHKPKHYRSVFSLFPDNMRNIDMQAV
                     GRLDADTTGVLLITNDGKLNHNLTSPSRKIPKLYEVTLKHPTGETLCETLKNGVLLHD
                     ENETVCAADAVLKNPTTLLLTITEGKYHQVKRMIAAAGNRVQHLHRRRFAHLETENLK
                     PGEWKFIECPKF"
     gene            complement(8727..10703)
                     /locus_tag="WIC03_000684"
     CDS             complement(8727..10703)
                     /locus_tag="WIC03_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEZ3883220.1"
                     /translation="MSKLKTIALTASGLSVCPGFLYAQNTSSHQIGLAIMRLNSSILD
                     LPPTKQYFQSGSLWSELRQGFRMGEVNPELVRRHESKFIASHSYFNRVINRSRPYMYH
                     IANEVKKRNMPAEAALLPFIESAFVTKAKSHVGASGLWQFMPATGRHYGLEKTPVYDG
                     RHDIYAATDAALNYLQYLYGLFGDWPLAFAAYNWGEGNVGRAINRARAQGLEPTYENL
                     RMPNETRNYVPKLLAVRNIIATPQSFGMNISDIDNKPYFQAVEPDRPLDNEAIVRLAG
                     ITQSELLALNPAFNVPAFIPKSKRKLLLPVASVQTFQSNYLNAAPDSLFSWEVYTPAA
                     KTSLSDISTATGMSIADIKRLNNLNGNLVNAGRSILVAKNGKTLQTASESVVSIDIDN
                     TPDTYRSNMPAGTVNVGIARIRPAAAQTADITVAPLPQKTVRTEPDPLVRIAEPALAT
                     AAAQPQTEKQTAMPSETQTATLAQIIPQNDMQAADELMQLVARNNLRRQAEETISAVI
                     GTPDTVAEHNISSSPQHTVAADGKRRARLETRVAKAADGEAEISPLHASIHRVVEGDT
                     LFNIAKRYNVSVADLIVANNIKGNTIQKGQVLRLAQAAPAQTRIEKVSYTARKGDTFK
                     SIAARFNIHIDDIRRLNPNLNTINPGQRVKLIGS"
     gene            10881..>11159
                     /locus_tag="WIC03_000685"
     CDS             10881..>11159
                     /locus_tag="WIC03_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEZ3883221.1"
                     /translation="MDAWFEDTAMGRYVAKLEQDFFGRYLDSYRFSGMCAVQVGGPWL
                     SLSEDVVCVPRDMSMSAENMALADVSADMLLFPHTLEGGVPSQILSEAH"
BASE COUNT         2610 a         2544 c         2998 g         3007 t
ORIGIN      
        1 agagtcggct tcttccggct cagacggcat acgtttatgc cggcacttct taccgccctc
       61 gtgccggcta tcccaaaact gctttgaaca ctgaacgctc ttcggtttac ttcaaaaacg
      121 cggcgtgata agcaatatgc tcgccaataa aactggcgat gaagtaatag ctgtgatcgt
      181 agcctttatg gaaacgcaca tcgaccggct ggtttgccgc acggcaggtt tcgataaaat
      241 cttcggtacg caattgtgtc ggcaaaaact catcttccaa gccttgatcg atgcgcatac
      301 cttgcacttt atagccttgt tgaatgagtg agttcgcatc atattgctgc catttttcgc
      361 gatcttttcc taaataagca gtaaaggctt tttctcccca cggtacgagg cttggcgata
      421 aaataggcga aaaggcagaa acactttgat aatgttcccg attccgtagt gccaatacca
      481 atgcgccgtg tccgcccatc gaatgcccca taatggaacg tttgccgttg gtaggaaagt
      541 gtttctcaat cagacggggc agctcgttca aaatgtaatc atacatttga taattcgccg
      601 cccaaggctg ttcggtcgca ttcaaataaa agcctgcacc ctgtcctaaa tcgtaagcat
      661 catcgttcgg cacttgctct ccgcgagggc tggtatcgga ggccaccaca attacttgat
      721 gttctgccgc ataacgctga aagcctgatt tggtaatgaa attttgttcc gtacacgtca
      781 agccggaaag ccaataaatc acaccaagcg gtcgattttc tggattattt ggcaaataga
      841 cggcaaattt catttcgcat tgcagcgttt gggcatgatg cgcccaaact tgttgcgaac
      901 cgccaaaaat ttgatgttgt tcaatcagtt tcatcgcata ccttagtagt gaataacggc
      961 gcggatcgat ttaccttcgt gcattaactc aaaggcttta ttgatttgat cgagtgtcat
     1021 tgtgtgggtt acaaacggtt ctaactcaat gtcgcctttc attgaatctt ccaccatttt
     1081 cggaagctcg gagcggcctt tcacaccgcc aaatgctgaa cctttccaaa cacgacctgt
     1141 taccaactgg aacggacgcg ttgaaatttc ttgtcctgca cctgctacgc cgatgataat
     1201 ggattgaccc caaccacgat gtgcactttc taatgcttga cgcattacgt ttacattgcc
     1261 gatacattca aaggtatggt caatgcccca tttgttgatg tctaacaaca catctttaat
     1321 tggtttatcg taatcgttcg ggttcaaaca atccgttgca ccaaactgtt ttgccaactc
     1381 aaattttgcg ggattggtat caatggcgat gatgcggcct gctttggctt ggcgcgcacc
     1441 ttgcaccaca gccaaaccaa tcgcgcctaa gccaaacacg gcaacagagt cgccttcttg
     1501 cacttttgcc gtattatgca ccgcgccaat acctgtggta acgccgcagc cgagcaaaca
     1561 tacttgttca tggttggctt cagggttgat tttcgccagt gaaacttcgg caacaacgga
     1621 gtattcactg aaagtcgaac agcccatata gtgataaatc ggctgacctt gataagaaaa
     1681 acgcgtcgtg ccgtccggca ttaagccttt accttgtgta tcacgcactg agacgcacaa
     1741 gttggtttta cctgaacaac aaaactcaca ttcgccacat tcggcggtgt aaagcggaat
     1801 cacgtgatca cccggtttta cgcttgacac accttcgccc acagcaacga ccacacccgc
     1861 accttcgtgt ccaagcacca cagggaatac gccttcagga tcgcttcctg ataacgtaaa
     1921 cgcatcagta tggcacacgc cagtgtgggt attgcggatt aacacctcgc ctttacgcgg
     1981 catttctacg tcgatttcca caatttgtaa gggttggtta ggggcgaatg ccaccgccgc
     2041 acgagatttg atggttgaat cggtttgttt catttccatt gtctttcctc ttaattcatt
     2101 gacaaagttg ccttacctga gcaacatagc aacagtttac actttagagt taactctaaa
     2161 gcaagtgttt tttactgttt ttgtttagaa ggagggaaaa tgacttatac cactgccaaa
     2221 gccgccgaaa aaataggcat ctccgcccac accctacgtt tttacgacaa agaaggtttg
     2281 ttgcctaata tcggacgtga tgaatacggt aaccgctgtt ttaccgataa cgatttgcaa
     2341 tggttgggct tattgcaatg cttgaaaaat acggggatga gcttaaaaga catcaaacgc
     2401 tttgcggaat gtaccgtcat tggcgacgat accattgaag aacgcctttc cttgtttgaa
     2461 aatcaaatag aaaatgtgaa gtgtcaaatt gccgaattaa aacgctattt agatttgctt
     2521 gaatacaaat tggcgtttta ccaaaaagcg aaagcattag gttcggtaaa agccgtaaat
     2581 ttgccgcaaa ttcctgaaac ggcttagttt tagtgcaata agcaggattt ttgtcctgtt
     2641 cgtaatagaa atcaaaaatt tgttgtttaa aaagcggatt gtaagcaatg atatagtgga
     2701 ttaacaaaaa tcaggacaag gcgacgaagc cgcagacagt gcaaatagta cggaaccgat
     2761 tcacttggtg cttcagcacc ttagagaatt gttctctttg agctaaggcg aggcaacgcc
     2821 gtactggttt ttgttaatcc actataaaaa aatccacgct ctaatccgtt gcttgcttgg
     2881 ctcaaggtat tgaagcagcg gatgaagtta aaccacagaa aaaacgccgc gtaacagcgg
     2941 cgttttgatg ctaaggttta attggcattt tcttttaaag ccaagtcgac ccgctcgcgc
     3001 aattctttac cgggcttgaa gtggggtaca tgtttttcag gtacttccac acgctcgccg
     3061 gttttgggat tgcgaccgat gcgggcagga cgatggttca aatcgaagct gccgaaaccg
     3121 cggatttcga tgcgttgacc tcgggcaagc gatctagtca tggtgtcaac caagactttt
     3181 acgctgtact ctacgtcttt tgccagaaga tgcgtgccgt ttttggcggc aaacacttct
     3241 gccaaacgaa ccattaactc agactttgtc atgtctgcaa ccttattctt gttcgccgga
     3301 gagtttggct ttcagcaggt cgcccaagct ggtggtgccg gcattcgcat tggcggcggc
     3361 attgacggag ttcagtgctt cgcggctttc tttggcatct ttggctttaa cggaaagttt
     3421 gatgctgcgg tttttgcggt caacggtaac gatgacggct tcaacttcgt cgccttcttt
     3481 cagtttggtg gtcaaatctt caacgcggtc ggctgcaaat tcggaagcag gcaggtagcc
     3541 ttctacttcg tcagacaggg cgataacagc acctttggcg tcaacagatt tcacggaacc
     3601 tttaaccaaa gaacctttgt cgttcacgct gatgaagttg ccgaacggat cgccttccag
     3661 ttgtttgata cccaaggaga tgcgttcttt ttccacgtcg attgccaata cgacggcttc
     3721 gacttcttcg ccttttttgt atttgcgtac ggcttcttcg ccggattcgg tccaggacag
     3781 gtcggacagg tgaaccagac cgtcgatgcc gccgggcagg ccgacgaata cgccgaaatc
     3841 ggtaatggat ttaaccgcac cggagatttt gtcgcctttg ttgtggttgg cggcaaattc
     3901 ttcccaagga ttggcttggc attgtttcat acccaaagag atacggcggc ggccttcgtc
     3961 gatttccaaa atcatgactt cgacttcgtc gcccagttgt acgactttgc tcgggtgtac
     4021 gtttttgttg gtccagtcca tttcggagac gtgtaccaaa ccttcgatgc cttgttcgat
     4081 ttcgacgaat gcgccgtagt cggtcaggtt ggatactttg ccgaacaggc gggtgccttg
     4141 cggataacgg cgggtcagac cgctccaagg atcttcgccc agttgtttca tacccaagga
     4201 aacgcgttgt ttttcttggt cgaatttcaa tactttggct tcaacttcct gaccgacttc
     4261 caagacttcg ctcgggtgtt tcacacggcg ccatgccaag tcggtgatgt gcagcagacc
     4321 atcgatgccg cccaagtcaa cgaatgcacc gtaatcggta atgtttttaa cgatgccttt
     4381 gatgacggag ccttcttgca ggttttccag cagggctttg cgttcttcac ccaaggtagc
     4441 ttccaggacg gcgcggcggg acacaacaac gttgttgcgt tttttgtcca gtttaatcac
     4501 tttgaattcg atctctttgc cttcgaagtg agaagtgtct tttacaggac gtacgtcgac
     4561 caaagaaccc ggcaggaatg cgcggatgct gctaatcata acggtcaggc cgcctttgac
     4621 tttgccgttg atgatgccgg acaggatgtt gccgttttcc atggcttctt ccagggcaat
     4681 ccaatcggca gcgcgtttgg ctttttcgcg ggacagtttg gtttcgccga agccgttttc
     4741 gacggattcg atggtaacgg taacgaagtc gccgacttta acttcaattt cgccttgagc
     4801 gtttttgaat tcagctacat caatcaggga ttctgatttc agacctgcgt ttacggtaac
     4861 gaagttttgg tcgattgcca ctacttcagc ggtaatcacc tcacccgggt tcatttcttg
     4921 cagggtaaag ctttcttcca acagctgagc aaaattttcc atagacatat ataactcttt
     4981 tcggtacacc gccaaggggt gcggggtggg ttggtggatg tctgccgtcc ttggcagggc
     5041 agacgggtaa ataaggtttc agacggcata tggggaaatt catgctgtct gaaacgcaat
     5101 gcgggcgatt atacctgaaa atttaaactt tacgatacca atcaagcact ttttttacag
     5161 tttcttctat agtcaggcgg ctcgtatcca aaagcagggc atcgggttgt tgtttcaggg
     5221 gggcaacctt gcggtttcgg tctgcctcgt ctctggcttc gatgtcggac aggatgcgct
     5281 cgaatgccaa accttcgcag gggatgccga tttgtttggc gcggcgttcg gcacggattt
     5341 tggactctgc cgtcaggaag attttaagtt cggcttgggg gaagacgacc gatccggtgt
     5401 cccgtccgtc ggcgaccagt cctttttcgg tcagaaaatc gcgttggcgt tgcagcaggg
     5461 cggcgcggac tttaggcaac tgtgcgactg cggatgcgcc catgccgatg gcttctgtcc
     5521 ggatgccgtc tgaaacgtct tcgccgtcga gcaggatgcg gctgcctgaa aatacggcgg
     5581 gcagtttttt tgccagtttg gaaacgtttt cttcatcgtg ccattccacg ccttgttttt
     5641 gtgcatatag ggcggtcagg cggtagagtg cgccggtatc gagataatcg tatcccaatg
     5701 cggcggcaac gcgggcggca accgtgcctt tgcccgatgc gcccgggccg tcgatggcga
     5761 tgactttttg tctgttcata agggggattc ctgatggttt ggggtatggg ttttgccgtc
     5821 tgaaggatgt gtttcccgtt ggggcggatt ctacctgttt taaaggacga ttgtctaagc
     5881 agacggaacg ccgcctgccc gaaaacatcc gacagaagcc ggcaagccgg ttcggattcc
     5941 ggcgttgcgg gtattgtggc gggcatcaag gcaatgctgt ctgaaagagg tatgaccttc
     6001 agacggcatc gattgctgca gattagaaca ggttgctgac gaaaatcagc agaatcacca
     6061 gcggcacaag atatttcaca taagcaaacc aaatattgac cgtcgtatgg ttgcctttgt
     6121 aaagcaattc ttccttcgct tcgtccttca tcacaaaacc gacaaacagc gcgaaaccga
     6181 gcgcggtcag cataaacaag atgttgccgc tgatgtagtc gaaggcatcg aaaatatttt
     6241 tgccgaacac ggaacgtctt tccacgaacc atagctcaga atggacggga tgttgccgaa
     6301 aatgaagatg gcggctaata caatcgtaat cgcggcggta cggcggattt tggttttttc
     6361 ctgaatggtc gtaatcaaca cttcataaat ggtcagcgaa gtcgtcaatg cggcaatcag
     6421 gagcagcgag aagaaaatca cggcgaacac agatctcgcc cacatatgtg agaacacaat
     6481 cggcaagctt tggaacacca aagtcgggcc ggaatcgggg gcaacgccga agctgaagag
     6541 cgacgggaaa atcataaagc ccgcaagtat ggcgatgatg gtattggtaa ttgccgtgat
     6601 aactgccgtc tgaaccagat tttcgttttt atccaaatag ctggacaagg taatcatcac
     6661 gccgaaaccc aggctcaggg caaaaaatac ctgccccaaa acgaagacga acagttcggc
     6721 ggtaatcttg ctgaaatcag gtttcagata gaaagcaacc ccttccattg cgcccggaag
     6781 ggtaacgttg cggacgacca tcgcgattag gaacaaaaac agcagcggca tcagctattt
     6841 tgccgctttt tcaatgccgc cgataacgcc tttgaccaaa atccattggt tcacggcgac
     6901 aaaaagcagc gtataaaacg caatttccca agggctgttt caatgtgttc ggcaaagaag
     6961 ccttttgtaa ccacaccgtc gacggggctg gaaatattca aatttcctcc aataatatta
     7021 acgatatagc tgattaccca gccgccgagt accatgtaat aagccatgat gccgaacgcg
     7081 ccgagcagcc catccagccg accagtttcc aaattttggc aatgggtttg ccgttcatcg
     7141 ggccgccgaa cgcatccagc gcgttcacgc ctttgcgccg tccgatgaca ttttccacca
     7201 aaatcatcgg gatgccgata accagcatcg cgatacagaa taaaaacaca tacgcgccac
     7261 caccgttttc accgaccaaa tacgggaaac gccacgtcgc gccgaaaccg acagtcgcgc
     7321 cggcaatggt caggatatag gttaatcggc tggatcaggt ttgacgattg gtatttgaag
     7381 gggaagacat attgaaaccg tgtccgattg agataaagcg gaaattctac acgcgttttt
     7441 taacaggaac tgactgattg tttttcaact gtctagtgat tttccatgta aaaggcatat
     7501 atttcagtga gtatttctga tgagtataac ccgatgagga agaatcggag tttataaata
     7561 gattaatttg ttattcttct acatcggtgt atggaaatga aattttgtta attatattaa
     7621 tgatagcatt taaataatga tgaaagagag ggaatttgaa atatagtgga ttaacaaaaa
     7681 tcaggacaag gcgacgaagc cgcagacagt acaaatagta cggcaaggcg aggcaacgct
     7741 gtactggttt aaatttaatc cactataaaa aaaccatcta tacaagggga gaagtataga
     7801 tggcaaaaca cattacggtg aaaacgtctt actcataagc ctgcttgaac aggcgttact
     7861 cagacaaatg gattatatcg taaattgatt ttttgcgtta aattgggtta aaccatatat
     7921 ttaatgagta tgtggatgaa tataaatgga attcttggat gttatttcag aattttggac
     7981 attcgataaa tttccattcc ccgggtttga ggttttctgt ttccagatgt gcgaatcgcc
     8041 ggcgatgaag gtgttgcacg cggttgccgg cggcggcgat catgcgtttg acttggtggt
     8101 attttccttc ggtaatggtc agcagcaggg tggtcgggtt tttcaaaacg gcatcggcgg
     8161 cacaaacggt ttcgttttcg tcgtggagca gcacgccgtt tttcaaggtt tcgcagagcg
     8221 tttctcctgt ggggtgtttg agcgttactt cgtatagctt gggaattttt ctgctcggcg
     8281 aagtcaggtt gtggttcagt ttgccgtcgt tggtaatcag caatacgccg gtcgtatccg
     8341 catccagcct gccgaccgcc tgcatatcga tgttccgcat attgtcgggg aacaggctga
     8401 atacgctgcg gtagtgcttg ggtttgtgcg aagtttcgta atcttcaggc ttgttgagca
     8461 tgatgtagaa atagggttcg ggaacgacgg ttactgcttc cccgtcaata tccaacgttt
     8521 cgacggatga ggaatcgatg tctgcatcgg tgtcgtccat gcaggttccg ttgatgaaaa
     8581 cataaccgcc ggcaatcagc cattggcact gcttgcggct tcctatgcct tgatattgca
     8641 ggtatttgat aagtttcatg atggtatggg aaatgtggga tgaaaaagac aggactgaga
     8701 aagtcctgtc atgtgccgta tccgaatcag cttccaatca gtttgaccct ctgtcccgga
     8761 ttgatggtgt tcaggttggg attgagccgg cggatgtcgt cgatatggat attgaagcgc
     8821 gcggcgatac ttttgaaggt gtcgcctttg cgcgcggtgt aggatacttt ttcaatacgg
     8881 gtttgggcag gggctgcctg cgccaggcgg agtacctgtc ctttttggat ggtgttgcct
     8941 ttgatgttgt tggcgacaat caggtcggct acgctgacgt tgtagcgttt ggcaatgttg
     9001 aacagggtgt cgccttctac aacgcggtgg atgctggcat ggagcgggga gatttcggct
     9061 tcgccgtcgg cagctttggc tacgcgcgtt tccaaacgtg cccggcgttt gccgtcggcg
     9121 gcaacggtat gttgcggaga tgaggaaatg ttgtgttcgg caactgtgtc aggcgtgccg
     9181 atgacggcgg agatggtttc ttcagcctgt cggcgcaggt tgtttcgggc aacaagctgc
     9241 atgagttcgt ctgccgcctg catgtcgttt tgggggatga tctgcgcgag tgttgcggtt
     9301 tgggtttcag acggcatggc ggtctgtttt tcggtttgag gttgcgctgc ggctgtcgca
     9361 agggcaggtt cggcaatacg gacaagggga tcgggttccg tacggacggt tttctgcggc
     9421 aaaggtgcga cggtaatgtc cgctgtctgt gcggcggcgg gtcggattcg ggcaatgccg
     9481 acgttcaccg tgcctgccgg catattggaa cggtaggtgt cgggcgtatt gtcgatgtcg
     9541 atggaaacga cggattccga tgccgtctga agggttttgc cgttcttggc gacaaggatg
     9601 ctgcgtcctg cgttgacaag gttgccgttc aggttgttga ggcgtttgat gtcggcaatg
     9661 ctcatgccgg ttgccgtcga gatgtcggac aggctggttt tggcggcagg cgtatagact
     9721 tcccatgaaa acaggctgtc gggtgcggcg ttgaggtagt tgctttggaa ggtttggacg
     9781 gatgcgacag gaagcagcag tttgcgtttg cttttgggga taaacgcggg gacgttgaat
     9841 gcggggttta gggcgagcag ctcgctttgc gtgatgccgg caagccggac gatggcttcg
     9901 ttgtcgagcg gacgatccgg ttcgactgcc tgaaaatagg gtttgttgtc tatgtcgctg
     9961 atattcatac cgaaagattg gggagtggca ataatgttgc gcacggcgag cagcttgggg
    10021 acatagttgc gcgtttcgtt gggcatacgc aggttttcgt aggtcggttc gagcccttgg
    10081 gcgcgggcgc ggttgatggc gcgtccgacg ttgccttcac cccagttgta ggcggcaaag
    10141 gcgagcggcc agtcgccgaa cagtccatag aggtattgca gatagttgag tgcggcatcg
    10201 gtggcggcgt aaatgtcgtg cctgccgtcg taaaccggtg ttttttccag gccgtaatgc
    10261 ctgccggtag cgggcatgaa ctgccacagg cccgatgcgc cgacgtgtga tttggctttg
    10321 gtgacgaacg cgctttcgat gaagggaagc agggcggctt cggcgggcat attgcgtttt
    10381 ttgacttcgt tggcgatatg gtacatatag ggtctactcc ggttgatgac cctgttgaaa
    10441 tagctgtggc ttgcgatgaa tttgctttcg tggcggcgta ccagttcggg attgacttcg
    10501 cccatccgga agccttggcg cagctcgctc cacaggctgc cggattggaa atattgtttt
    10561 gtcgggggca ggtcgagtat tgaagagttt aagcgcataa tcgccaaacc gatttggtgt
    10621 gatgaggtgt tttgggcgta taggaaaccc ggacaaacgg acagacctga cgcggtcagg
    10681 gcgatggttt tgagtttgga catgatttat atcggcggaa taaacatatt ttatcgtccg
    10741 atggtactgt ttgactgaaa aagcgtcaag ttttacagga actttatgcg gattcggaag
    10801 tgggcggccg atgtgttttt tgacagaaat tctgtttgga atagaatatc ggcaggcaat
    10861 ctgtttgaaa cggacgcaat atggatgcat ggtttgaaga tacggcgatg gggcggtatg
    10921 ttgcaaaatt ggaacaggac ttcttcgggc ggtatctaga ttcataccgt ttttcgggaa
    10981 tgtgtgcggt tcaggtgggc ggtccgtggc tgagcctgtc tgaagatgtt gtctgtgtgc
    11041 cgcgagatat gtcgatgtcg gcggagaata tggctttggc ggatgtttct gcggatatgc
    11101 tgctttttcc gcatacgctt gaaggcggtg ttccttcgca aatcctgtcg gaagcgcac
//
LOCUS       DAWHSK010000038        11057 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.038, whole genome shotgun sequence.
ACCESSION   DAWHSK010000038 DAWHSK010000000
VERSION     DAWHSK010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11057
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.038"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(162..345)
                     /locus_tag="WIC03_000686"
                     /pseudo
     CDS             complement(162..345)
                     /locus_tag="WIC03_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(347..961)
                     /locus_tag="WIC03_000687"
     CDS             complement(347..961)
                     /locus_tag="WIC03_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEZ3883222.1"
                     /translation="MTIKSGGIADGYQCRLKDVVWKTLGEIAEYSKNRICSDKLNEHN
                     YVGVDNLLQNREGKKLSGYVPSEGKMTEYIANDILIGNIRPYLKKIWQADCTGGTNGD
                     VLVIRVTDEKVNPKYLYQVLADDKFFAFNMKHAKGAKMPRGSKAAIMQYKIPIPPLPE
                     QEKIVAILDKFDTLTHSVSEGLPYEIALRRKQYEYYREQLLAFR"
     gene            complement(933..1525)
                     /locus_tag="WIC03_000688"
                     /pseudo
     CDS             complement(933..1525)
                     /locus_tag="WIC03_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235659.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
     gene            complement(1647..1739)
                     /locus_tag="WIC03_000689"
     CDS             complement(1647..1739)
                     /locus_tag="WIC03_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-damage-inducible D domain protein"
                     /protein_id="HEZ3883223.1"
                     /translation="MTTENNAFENAKHIDETGNEYWSVCTLQTK"
     gene            complement(1856..3400)
                     /locus_tag="WIC03_000690"
     CDS             complement(1856..3400)
                     /locus_tag="WIC03_000690"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HEZ3883224.1"
                     /translation="MMTEMQQRAQLHRQIWKIADEVRGAVDGWDFKQYVLGTLFYRFI
                     SENFTDYMQAGDSSIDYAAMPDSIITPEIKDDAVKVKGYFIYPSQLFCNIAAEAHQNE
                     ELNTKLKEIFTAIESSASGYPSEQDIKGLFDDFDTTSSRLGSTVADKNKRLAAVLKGV
                     AELDFGSFEDHHIDLFGDAYEYLISNYAANAGKSGGEFFTPQSVSKLIARLAVHGQEK
                     VNKIYDPACGSGSLLLQAKKQFDEHIIEEGFFGQEINHTTYNLARMNMFLHNVNYNKF
                     HIELGDTLTNPKLKDSKPFDAIVSNPPYSINWIGSDDPTLINDDRFAPAGVLAPKSKA
                     DFAFILHALNYLSGRGRAAIVSFPGIFYRGGAEQKIRQYLVEGNYVETVIALAPNLFY
                     GTGIAVNILVLSKHKDNTDIQFIDAGSFFKKETNNNVLTEEHIADIVKLFADKADVPH
                     IAQNAAQQTVKDNGYNLAVSSYVEPEDTREIIDIKQLNAEIGETVAKIERLRREIDEV
                     IAEIEA"
     gene            complement(3473..4477)
                     /gene="rfaD"
                     /locus_tag="WIC03_000691"
     CDS             complement(3473..4477)
                     /gene="rfaD"
                     /locus_tag="WIC03_000691"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HEZ3883225.1"
                     /translation="MTIIVTGAAGFIGSNIVKALNQRGITDIVAVDNLSKGEKFKNLA
                     ECEIAHYLDKHEFIRQVREHILPYQNIEAVFHQGACSDTMNHDGLYMMDNNYQYTLDL
                     LDWCQDERIPFLYASSAAVYGKGEIFREERELEKPLNVYGYSKFLFDQVLRRRMKEGL
                     TAQVVGFRYFNVYGQHEQHKGRMASVTFHHFHQYREHGYVNLFGSNDGYGNGEQTRDF
                     VSVEDVAKVNLYFFDHPELSGIYNLGTGRSQQFNELAAATVNACRAAEGKPEMSLKEL
                     VEEELIRYIPFPDALKGKYQSFTQADITKLREAGYTEEFFDVKSGVERYVKWMLENLA
                     "
     gene            complement(4606..5637)
                     /locus_tag="WIC03_000692"
     CDS             complement(4606..5637)
                     /locus_tag="WIC03_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEZ3883226.1"
                     /translation="MPDFQRITIENIEYFIVDSIQNLRAEDSFIHRNNKLSVFGGNGE
                     ARKYVGSYIDDSGRKLSTFFEYENWGITETKNGRRVEYPFIQKNCFFNKKNLQRYLVD
                     AKVEYHKQEQKYHHDISRFYDDYVLSINNLPTENIFFSIHDVSDHLENSKGYRRGYIR
                     SEDDIWSIWRKIVLPKISYLSILKLLPVKDIEDSEPLFYFRIFLDYQFRSIVHPQLLS
                     REKLEIPASEFNQFVEQEIIKQKSRKGQQKYRKDVINHMSQCPFTLITDEILLRASHI
                     KPYMVCITEKNEKEALDYLNGLALTPTYDWLFDQGYITFLDDGRLICGTRLSRYTWEK
                     LNINPNAKN"
     gene            complement(5639..6874)
                     /gene="dcm"
                     /locus_tag="WIC03_000693"
     CDS             complement(5639..6874)
                     /gene="dcm"
                     /locus_tag="WIC03_000693"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEZ3883227.1"
                     /translation="MTISTMAQTHDTRLQKTLLFPSQQGWKSKTFLKPDSHIRLATVF
                     SGIGAVEQAFHRLNLNHTIVFSGDIDPYVKKSYLGNYKLNEDFWHNDITQFDARKFRN
                     QVDILVGGSPCQAFSMVGKRAGLEDTRGTLFYEFARVVDEVQPKIFIYENVKGLLNHD
                     NGKTWKVVKSVFYSLGYDLYFQIMNSKDYGIPQHRERIFVVGFHTPPINGFQFPEKIE
                     LEHTMQDFLEDYTDSKYFLREKGAKFVTSSKNRQKRYTQINGEIALCQKANQQFNWHG
                     DFIFQAARESEFDDFIFDVNNVEEKYYLSEKIKNYVLAGGTKNFKTSTETDLPVARPL
                     LQTMHKMHRAGVDNYVTHNRGRIRKLTPRECLRLMGFRDDFKILVSDTQMYRQAGNSI
                     VVDVLIAILKQMDITLYGE"
     gene            complement(6911..7882)
                     /gene="hldA"
                     /locus_tag="WIC03_000694"
     CDS             complement(6911..7882)
                     /gene="hldA"
                     /locus_tag="WIC03_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose synthase"
                     /protein_id="HEZ3883228.1"
                     /translation="MSAKFQQETLKSRFAQAKVLVVGDVMLDRYWFGDVSRISPEAPV
                     PVAKIGRIDQRAGGAANVARNIASLGGRAGLLSVTGNDEAADALDALMVQDGVASYLM
                     RDKQIATTVKLRVVARNQQLIRLDFEEHPNREVLEQIKRKYREILPEYDAIIFSDYGK
                     GGLSHISDMIDWAKHAGKTVLIDPKGDDYEKYAGATLITPNRAELKEVVGSWKNEGEL
                     TEKAQNLRRHLDLTAILLTRSEEGMTLFSEGEPIYQPTRAQEVYDVSGAGDTVIAGMG
                     LGLAAGCTMPEAMYLANTAAGVVVAKLGTAVCSFAELIKALSGQSTM"
     gene            complement(7937..8677)
                     /gene="pyrF"
                     /locus_tag="WIC03_000695"
     CDS             complement(7937..8677)
                     /gene="pyrF"
                     /locus_tag="WIC03_000695"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEZ3883229.1"
                     /translation="MNPLISDFQTPQQRTPVIVALDFSNEKDTLGFVRNLDPTLCQIK
                     IGKELFTATGRNLAESLINQGFKLFLDLKYHDIPHTVAQACKVAADMGVWMVDMHASG
                     GRRMMEAAAEAVAGYGTKPLLIGVTVLTSMEQSDLAEIGLNTAPEEQVIRLAKLAQSS
                     GLDGVVCSAQEAAPLRRELGQDFVLVTPGIRLDVAGNNDDQRRIMTPAEALAAASTYL
                     VMGRPVTQAADPVAVLREVNRVANLEAN"
     gene            complement(9203..9850)
                     /gene="adk"
                     /locus_tag="WIC03_000696"
     CDS             complement(9203..9850)
                     /gene="adk"
                     /locus_tag="WIC03_000696"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEZ3883230.1"
                     /translation="MKALFLGAPGAGKGTQAQFITAAFGIPQISTGDMLRAAIKAGTP
                     LGLEAKKIIDEGGLVRDDIIIGMVKERIAQDDCKNGFLFDGFPRTLAQAEAMVEAGVD
                     LDAVVEIDVPDSVIVDRMSGRRVHLASGRTYHVTYNPPKVEGKDDVTGEDLIQRDDDK
                     EETVKKRLAVYHEQTEVLVDFYSKLEGEHAPKYIKVDGTQPVEAVKAEVLGALGK"
     gene            10065..10904
                     /gene="htpX"
                     /locus_tag="WIC03_000697"
     CDS             10065..10904
                     /gene="htpX"
                     /locus_tag="WIC03_000697"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HEZ3883231.1"
                     /translation="MKRIFLFLATNIAVLVVINIVLAVLGINSRGGTGSLLAYSAVVG
                     FTGSIISLLMSKFIAKQSVGAEVIDTPRTEEEAWLLNTVEAQARQWNLKTPEVAIYHS
                     PEPNAFATGASRNSSLIAVSTGLLDHMTRDEVEAVLAHEMAHVGNGDMVTLTLIQGVV
                     NTFVVFLSRIIANLIARNNDGSQSQGTYFLVSMVFQILFGFLASLIVMWFSRQREYRA
                     DAGAAKLVGAPKMISALQRLKGNPVDLPEEMNAMGIAGDTRDSLLSTHPSLDNRIARL
                     KSL"
BASE COUNT         2624 a         2608 c         2591 g         3234 t
ORIGIN      
        1 gctgcggcgg aatgcgggtc ggttgtgtcc atcgtaggat gggcttcagc ccaccgcccc
       61 gcctgccgtt gttttactgt tgttgcagct tgctgctgtt tcggtgggct gaagcccacc
      121 ctactttgtc tgcgacttct accccaccga tgccgtctga atcatctcaa tcggtttttt
      181 ctctttgttt tgagcatccg ccgccatatt ttccggtttc cacacgctgt ggcggaatgc
      241 ggggcggttg ccgctcgccg tagggtgggc ttcagcccac cgccccgcct gccgttgttt
      301 tactgttgtt gcagcttgct gctgtttcgg tgggctgaag cccaccctac cgaaaggcaa
      361 gcagctgttc gcggtaatat tcgtattgtt tgcggcgcag ggcgatttcg tacggcaggc
      421 cttcgctgac ggagtgggtc agggtgtcga atttgtctag gatggcgacg attttttcct
      481 gttcggggag tggtgggatg gggattttat attgcataat cgctgctttg ctgccacgcg
      541 gcatttttgc acctttggcg tgcttcatat taaaggcaaa aaatttatca tcggccaata
      601 cctgatacag atattttgga ttaacctttt catctgttac ccggataacc aaaacatcac
      661 cgtttgttcc gcccgtgcag tctgcctgcc agattttttt caaatacgga cgaatatttc
      721 caatcaagat gtcattggca atatattctg tcatttttcc ctcacttgga acataaccgg
      781 ataacttttt accttctcta ttttgtaaga gattatccac gcccacgtaa ttatgttcgt
      841 tcagtttatc cgaacaaata cggttttttg aatattcggc aatctccccc aacgtcttcc
      901 aaaccacatc tttcagacgg cattgatagc catcagctat cccccccgat ttgattgtca
      961 aaatctaaaa gaaagtcgcg gtaataccgg tattggcgtt tgcgcagggc taattccgct
     1021 tccagcgtag cttccagctc ggtgaatttg tcaagtattt ttacaatttt ttgttgggtt
     1081 tctaggggcg ggatgggaat aagtaagttt tgcaattcac ttttagcaag tgctggtata
     1141 cctgctccgt attgacattg catgaatgta atttgcttca tttttaaaaa taatatgcaa
     1201 accgttgcaa tagtttgtct tcgttcgctt taatagtata tgcgtgagct cctgcaaaaa
     1261 aagggatatc catccaatta acaaaccccg cacttgctcc tccttggcta ataacaatcg
     1321 tgccagcttc gttattgtat tcatgccaat aacctgatgg attaataccg ccattgatta
     1381 cgggatactt atctttttct ctaagtagtg atttattaag ctgctttccc tttcctatac
     1441 ttgccacttc ccccaacggc ttccactcca ccggtgccgt ctgaatcatc tcaatcaatt
     1501 ttttcgcttt actttgtata tccatggttg ttttccgttt ctttcccgct gcggcgggat
     1561 gtggggcggt tatccgccgt agggtgggct ccagcccaac aatccgcctg ctgttttgcc
     1621 gttgtttcgg ggggctgaag cccaccctac tttgtctgca aggtgcacac cgaccaatat
     1681 tcattgcctg tttcgtcgat gtgtttggcg ttttcaaaag cgttgttttc tgttgtcatg
     1741 tatttttccc cgaaaatttc ttgatttgaa cttgtaaaga ttacttacag gttaccgccc
     1801 gtattttcag acggcctctt tcccattcag ttgttgcaaa atatgcaaca actgcctagg
     1861 cctcaatctc tgcaatcact tcgtcaattt cgcgccgcag ccgttcgatt ttggcgacgg
     1921 tttcgccgat ttcggcgtta agctgtttga tgtcgataat ttcgcgtgtg tcttcgggtt
     1981 caacatagct gctgacggcg aggttgtagc cgttgtcttt gacggtttgc tgggcagcgt
     2041 tttgggcgat atgcggcaca tcggctttgt cggcgaagag tttgacgatg tcggcaatgt
     2101 gttcttcggt taagacgttg ttgttggttt cttttttaaa gaagctgcct gcgtcgataa
     2161 attggatgtc ggtattgtct ttgtgtttgg acaaaaccag gatattgacg gcgatgccgg
     2221 tgccgtaaaa gagattgggc gcaagggcaa tcacggtttc cacgtagttg ccctccacca
     2281 gatattggcg gattttctgc tctgcgccgc cgcgatagaa aataccgggg aaagagacga
     2341 tggcggcgcg gcctctgccg gaaaggtagt tcagtgcgtg caggatgaag gcaaaatcgg
     2401 ctttggattt cggtgcgagt acgcctgcgg gggcaaagcg gtcgtcgttg attaaggtgg
     2461 ggtcgtcgct gcctatccag ttgatggaat aaggcggatt ggaaacgatg gcatcaaagg
     2521 gtttgctgtc tttgagcttg ggattagtca gtgtgtcgcc caattcgatg tggaatttgt
     2581 tgtaattgac gttgtgcaga aacatattca tgcgggcgag gttgtaggtg gtgtggttga
     2641 tttcctgccc gaagaagcct tcttcgatga tgtgctcgtc aaactgtttt ttcgcctgca
     2701 agagcaggct gcccgagccg caagctgggt cgtagatttt gtttactttc tcctgcccgt
     2761 gcaccgccag ccgcgcaatc agcttggata cgctttgcgg ggtgaaaaat tcgccgccgg
     2821 atttgcctgc gttggcggcg tagttggaaa tcaggtattc gtaggcatcg ccgaaaaggt
     2881 cgatgtggtg gtcttcaaaa ctgccgaaat caagttccgc cacgcctttg aggacggcgg
     2941 caaggcgttt gttcttgtcg gcaactgtgc tgccgagccg gctgctggtg gtatcgaagt
     3001 cgtcaaacag gcctttaatg tcttgttcgg acggatagcc ggaggcggag ctttcaatcg
     3061 cggtaaagat ttctttcagc ttggtgttga gctcttcgtt ttgatgggct tcggcggcaa
     3121 tattgcaaaa aagctggctg gggtagatga aatagccttt aactttgacg gcatcgtctt
     3181 tgatttcggg cgtgatgatg ctgtccggca tagcggcgta atcaatactg ctgtcgcctg
     3241 cctgcatata gtcggtgaag ttttcgctga taaagcggta gaaaagtgtg ccgagaacgt
     3301 attgtttaaa gtcccagcca tccaccgcgc cgcgtacttc gtcggcaatt ttccaaattt
     3361 ggcggtgcag ttgggcgcgt tgttgcattt cggtcatcat cgaaatccat ataaaagtta
     3421 aacaaatcaa aatcgcctga tattttcaga cgatttttta cgggcattca aattaagcca
     3481 aattttccag catccatttg acgtagcgct cgacgcctga tttgacatcg aaaaattctt
     3541 ccgtataacc tgcctcacgc aatttggtga tgtcggcttg agtgaagctt tggtatttgc
     3601 ctttgagcgc atcgggaaat gggatgtagc ggataagttc ttcttctacc agctctttca
     3661 agctcatttc gggtttgcct tcggcggcgc ggcaggcgtt gacggtggcg gcggcgagtt
     3721 cgttaaactg ttggctgcgg ccggtgccga ggttgtagat gccggaaagt tcgggatggt
     3781 cgaagaagta gaggttgact ttggcaacgt cttcgacgct gacaaagtcg cgggtttgtt
     3841 cgccgttgcc gtagccgtcg ttgctgccga acaggttgac gtaaccgtgt tcgcggtatt
     3901 ggtggaaatg gtggaaggtg acggatgcca tgcggccttt gtgttgttcg tgttgtccgt
     3961 aaacgttgaa gtagcggaag ccgacgactt gggcggtcaa gccttctttc atacggcggc
     4021 gcaatacttg gtcaaacagg aatttggagt agccgtagac gttgagcggt ttttcgagtt
     4081 cgcgctcttc gcggaagatt tcgcctttgc cgtaaaccgc cgcactggag gcataaagga
     4141 aagggatgcg ttcgtcctga caccagtcca gcaaatccag cgtgtactgg tagttgttgt
     4201 ccatcatata caaaccgtcg tggttcatcg tatcggaaca cgcgccttga tggaaaacgg
     4261 cttcgatgtt ttgataaggt aaaatgtgtt ccctcacttg gcggatgaat tcgtgtttat
     4321 cgagataatg ggcgatttca cactcggcaa ggtttttgaa tttttcgcct ttgctcaaat
     4381 tatcgacggc gacgatgtcg gtaataccgc gttgattaag tgctttgacg atgttgctgc
     4441 cgataaagcc ggccgcgcct gttacaatga tggtcatatt gggtttcctt tgttttgagt
     4501 aaaattaata aacttagtta caagaaatca tcgatattgt cctgaaacac aaatttgcga
     4561 tgatattcca aatatttttc tcttccttcg ggatatattc tcatcttaat tttttgcatt
     4621 tggattgata ttaagttttt cccatgtgta acggcttagt cgagtaccgc aaatcaaacg
     4681 accatcatcc aagaaagtaa tataaccttg atcgaacaac caatcataag tcggcgttaa
     4741 agctaaccca tttaaataat ctaatgcctc tttctcattt ttctcagtaa tacaaaccat
     4801 ataaggttta atatgactag cccttaacaa aatctcatct gtaattaatg taaatggaca
     4861 ttgcgacata tgatttataa catccttgcg atatttctgc tgcccttttc tacttttctg
     4921 tttaatgatt tcttgctcta cgaattgatt aaattctgaa gccggtattt ctaatttttc
     4981 tcttgataag agttgcgggt gcacgataga gcgaaattga taatccaaaa atattcggaa
     5041 ataaaataat ggttctgaat cttctatatc tttgacaggc aatagtttaa gaattgataa
     5101 ataactaatt ttgggcaata caatcttacg ccaaattgac catatatcat cctccgaacg
     5161 gatataaccc ctacggtaac ctttagaatt ttccaaatgg tctgatacat catggattga
     5221 aaagaaaata ttttctgtag gcagattatt aatacttaaa acgtaatcat cataaaacct
     5281 tgaaatatca tgatgatatt tctgttcttg tttgtgatat tcaacttttg catcaaccaa
     5341 ataacgctgt aaatttttct tattaaaaaa acaatttttc tgaatgaaag gatattctac
     5401 cctccttcca ttttttgtct cagtaatccc ccaattttca tactcaaaaa atgtacttag
     5461 ttttcgacca ctatcatcaa tataactacc tacatatttg cgtgcttccc cattaccacc
     5521 aaaaacagat aacttgttat tcctgtgaat aaaactatct tccgcacgta aattttgaat
     5581 actatcgaca ataaaatact ctatgttttc aatagtaatt ctttgaaaat ccggcatttt
     5641 actctccata aagcgtaata tccatttgtt ttaatatagc gattaataca tcaacaacaa
     5701 tactgtttcc cgcttggcga tacatttgag tatcactaac taaaatttta aaatcatctc
     5761 taaaacccat tagccgcaaa cattccctag gtgttaattt acgaatacgt ccacgattat
     5821 gagtaacata attatcaacc ccggcacgat gcattttatg catagtttgc aataatgggc
     5881 gagctacagg caaatcagtt tccgtactgg ttttaaaatt ttttgttcct cctgccaaaa
     5941 cataattttt gattttttca gaaagataat atttttcctc aacgttattt acatcaaaaa
     6001 taaagtcatc aaattcagat tcgcgggctg cctgaaaaat aaaatcacca tgccaattaa
     6061 attgttgatt tgctttttgg cataaggcaa tttctccatt aatctgtgta taacgttttt
     6121 gtctattttt agaactggta acaaatttcg cacccttttc acgtaaaaaa tatttgctat
     6181 cagtatagtc ctccaaaaaa tcttgcatag tatgttctaa ttcaatcttt tcaggaaact
     6241 gaaaaccatt tataggaggg gtatgaaagc caacaacaaa aatacgctca cgatgttgag
     6301 gaatcccata atccttacta ttcattattt ggaaatataa gtcataacca agtgaataaa
     6361 aaacactttt tacaactttc caagtttttc cattatcatg attaagcaag ccctttacat
     6421 tttcataaat aaatatcttt ggttggactt catccacaac gcgagcaaat tcatagaata
     6481 atgttcctcg tgtatcttct aatcctgcac gtttgccaac catggaaaat gcttggcaag
     6541 gactgcctcc aactaaaata tcaacttgat ttctaaactt tctcgcatca aattgagtaa
     6601 tgtcgttatg ccaaaaatct tcatttaatt tatagtttcc aagataactt tttttaacgt
     6661 atggatcaat atctcctgaa aaaacaatgg tatggtttaa atttaatcgg tggaatgcct
     6721 gttcaaccgc accaatcccg ctgaatacgg ttgctaatct aatatgtgaa tcaggtttaa
     6781 gaaaagtttt agatttccaa ccttgttgac tgggaaagag caaagttttt tgtaatcgag
     6841 tatcgtgtgt ctgtgccatt gtcgaaatag tcatacttat atcgttctgt ttatcttatc
     6901 aatatgaaaa ctacattgtt gattgccctg acaatgcctt gatcaattcg gcaaacgagc
     6961 aaaccgccgt accgagtttc gccacgacaa ccccggccgc agtattggca aggtacatgg
     7021 cttcgggcat ggtgcagcct gccgccaaac ccaagcccat tccggcaatg acggtgtcgc
     7081 ccgcaccgga tacgtcgtaa acttcttggg cgcgggtggg ctggtaaatc ggttcgcctt
     7141 cactgaacaa ggtcatgcct tcttcgcttc gggtcagcaa aatcgcggtc aaatcgaggt
     7201 ggcggcgcag gttttgcgct ttttcggtca actcgccttc gtttttccaa ctgccgacca
     7261 cttctttcaa ttcggcgcgg ttgggcgtaa tcagcgtggc gccggcgtat ttttcgtaat
     7321 cgtcgccttt ggggtcgatt aatacggttt tgccggcgtg tttcgcccaa tcgatcatat
     7381 cggagatgtg cgacaggccg cctttgccgt agtctgaaaa aatgattgcg tcgtattcgg
     7441 gcaagatttc gcggtatttc cgcttgattt gttccaacac ttcgcggtta ggatgttctt
     7501 caaaatcaag gcggataagc tgctggttgc gggcgacgac gcgcagtttg acggtggtgg
     7561 cgatttgttt gtcgcgcatc agataggagg cgacgccgtc ctgcaccatc agcgcatcga
     7621 gcgcgtcggc ggcttcgtcg ttgccggtta cggacaacag ccctgccctg ccgcccaacg
     7681 aagcgatgtt gcgcgcaacg ttcgccgctc cgcccgcgcg ttggtcgatt cgtccgattt
     7741 tcgccaccgg cacgggggct tcgggcgaaa tacgggacac atcgccgaac caatagcggt
     7801 cgagcatcac gtcgccgaca accaggactt tggcttgcgc gaaacgggat ttgagggttt
     7861 cttgttggaa cttggcggac atattgcctc cgttgtattt tttcagacgg cctcagcctg
     7921 taaggccgtc tgaaaatcag tttgcttcaa ggtttgccac gcggttcact tcgcgcaata
     7981 cggctaccgg atcggcagct tgggttacag gacgacccat taccaaataa gtcgaagcgg
     8041 cagccaaggc ttcggccggt gtcatgatgc ggcgctggtc atcattattg ccggcaacgt
     8101 ccaagcggat gccgggcgtg accaagacaa aatcttgtcc caactcgcgg cgcagcggtg
     8161 cggcttcttg ggcggaacag accacgccgt ccaagcccga actttgcgcc agtttcgcca
     8221 agcggatgac ttgttcttca ggggcggtgt tcaaaccgat ttccgccaaa tcgctttgtt
     8281 ccatgctggt caacacggtt acgccgatta agagcggctt cgtgccgtat ccggcaacgg
     8341 cttctgctgc ggcttccatc atacggcggc cgcccgatgc gtgcatatcg accatccaaa
     8401 cgcccatatc ggcagcgact ttgcaggctt gcgcgacggt gtggggaata tcgtggtatt
     8461 tcaaatcgag gaaaagtttg aaaccttgat tgattaagct ttctgccaaa ttgcgtcccg
     8521 tcgcggtaaa cagctctttg ccgattttga tttgacacaa tgtcgggtca aggttgcgga
     8581 cgaatccgag cgtgtctttt tcgttggaaa aatcaagggc gacgataacg ggggtgcgtt
     8641 gttgcggagt ttggaagtcg gagattaaag gattcatggt gttttcgttt cgggttttca
     8701 gacgaccttt atcgtctttt tcgcagtata gtggattaac tttaaaccgg tacggcgttg
     8761 tctcgcctta gttcaaagag aacgcttctc taaggtgctg aagcacaaag tgaatcggtt
     8821 ccgtactgtt tgtactgcct gcggcttcgt cgccttgtcc tgatttttgt taatccacta
     8881 taagaacgta aattcaaacc gtaggtcggg cattgatgtc cgacctacgc ggactcaagc
     8941 ctccgacaaa ctgtttcgga taaatttgaa ccggtacatc agccgtcatt cccgcgtagg
     9001 cgggaatcca ttgataaaac tcagttcatc agatttaaac agcgattgcc caaattcaga
     9061 aatggattcc cgcctgcgcg ggaatgacgc caaatgatta tttttcggtt gcaacacgct
     9121 gtttatcaaa aacaggtcgt ctgaaaacct gattttctgt tttcagagtg cgaagcctgc
     9181 ccgtgggtac gaccttttcg atttatttgc ccaatgcgcc caatacttcg gctttcacgg
     9241 cttctaccgg ctgagtgccg tcaactttga tgtatttagg cgcgtgttcg ccttccagtt
     9301 tgctgtaaaa atcgaccaaa acttcggttt gctcgtggta aacggcaagg cgttttttca
     9361 cggtttcttc tttgtcgtcg tcgcgctgaa tcaaatcttc gccggttacg tcgtctttgc
     9421 cttcaacttt gggcgggttg taggtaacgt ggtaagtacg gccggaagcc aaatgcacgc
     9481 ggcggccgct catgcggtcg acaatcacgc tgtcaggcac atcgatttca acgactgcat
     9541 ccaaatccac gcctgcttca accatcgctt cggcttgtgc caatgtgcgc gggaaaccgt
     9601 caaacaggaa accgtttttg cagtcgtctt gcgcgatgcg ttctttgacc atgccgataa
     9661 tgatgtcgtc gcgcaccaag ccgccttcat caatgatttt tttcgcttcc aaacccaagg
     9721 gcgtgcctgc cttaatcgcg gcgcggagca tgtcgccggt agagatttgc ggaatgccga
     9781 acgcggcggt gatgaattgc gcctgagtgc ctttgcccgc gcccggcgcg cctaaaaata
     9841 atgctttcat gaagtgatcc tttgaaatgt gtttattgga atcaaacaga tggtttacga
     9901 ttctacacgg cttggcacaa tagttcaaaa accaactgca caaactgcca tttgcaagca
     9961 aatgttttgc atacctttgc cgagtctgat attcacaagc cggcaacttt ttgggacaat
    10021 ccccaaaatt tcaaaacatc acaccctacc aaaaaggaac atccgtgaaa cgaatttttc
    10081 tgtttttggc taccaatatc gctgttttgg tcgtaatcaa cattgttttg gcggttttgg
    10141 gcatcaacag ccggggcggc acgggcagcc tgttggcgta ttccgccgtc gtcggcttca
    10201 ctggttcgat tatttcgctg ctgatgtcca aattcatcgc caaacaatcg gtcggcgcgg
    10261 aagttatcga cacgccgcgc accgaagaag aagcctggct tttgaacact gtcgaagccc
    10321 aagcgcggca atggaacctg aaaacgcccg aagtcgccat ctaccactcc cccgaaccca
    10381 atgcctttgc cacgggcgca tcgagaaaca gctccctgat cgccgtcagc accggtttgc
    10441 tcgaccatat gacgcgcgac gaagtggaag ccgtattggc gcacgaaatg gcacacgtcg
    10501 gcaacggcga tatggttacg ctgacgctga ttcaaggcgt ggtcaatacc ttcgtcgtgt
    10561 tcctgtcgcg cattattgcc aacctgattg cccgaaacaa cgacggcagc cagtcgcagg
    10621 gaacttattt cctagtcagc atggtattcc aaatcctgtt cggcttcctt gccagcttaa
    10681 ttgtcatgtg gttcagccga caacgcgaat accgcgccga cgcgggcgcg gcaaaactgg
    10741 tcggcgcgcc gaaaatgatt tccgccctgc aaaggcttaa aggcaacccg gtcgatttgc
    10801 ccgaagaaat gaacgcaatg ggcatcgccg gagatacgcg cgactccctg ctcagcaccc
    10861 acccttcgct ggacaaccga atcgcccgcc tcaaatcgct ttaaaccgat ttgaaacggc
    10921 aaaaaccgta cgcgcaagca gtacggtttt ttgtatatgc tgtctgaaaa catcaggttt
    10981 ccataccgac accaaccgta tccatttccg caccggatac gccgcattga tataccctga
    11041 taaaccgtat ccggaca
//
LOCUS       DAWHSK010000039        10829 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.039, whole genome shotgun sequence.
ACCESSION   DAWHSK010000039 DAWHSK010000000
VERSION     DAWHSK010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10829
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.039"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(73..1218)
                     /gene="dapE"
                     /locus_tag="WIC03_000698"
     CDS             complement(73..1218)
                     /gene="dapE"
                     /locus_tag="WIC03_000698"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HEZ3883232.1"
                     /translation="MTETQSLELAKELISRPSVTPDDRDCQKLLAERLHKIGFAAEEL
                     HFGDTKNIWLRRGTKAPVVCFAGHTDVVPTGPVEKWDSPPFEPAERDGRLYGRGAADM
                     KTSIACFVTACERFVAEHPDHQGSIALLITSDEEGDALDGTTKVVDVLKARDELIDYC
                     IVGEPTAVDKLGDMIKNGRRGSLSGNLTVKGKQGHIAYPHLAINPVHTFAPALLELTQ
                     EVWDEGNKYFPPTSFQISNINGGTGATNVIPGELSVKFNFRFSTESTEAGLKQRVHAI
                     LDKHGVQYDLQWSCSGQPFLTHAGKLTDVARAAIAEICGVETELSTTGGTSDGRFIKA
                     IAKELIELGPSNATIHQINENVRLNDIPKLSAVYEGILARLLAGNAV"
     gene            complement(1331..1951)
                     /locus_tag="WIC03_000699"
     CDS             complement(1331..1951)
                     /locus_tag="WIC03_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HEZ3883233.1"
                     /translation="MLTSKSCDLFNIPFFQFSQLKKYQPESIPQIKADYKENWQVWQQ
                     LIQQVAAELGAPFAPPHIERWCNGWQVRAHFFAYFKYEQYKNSAAILSILLNRRRLSV
                     SLDWHCYKADVSPIALPEYNRWLDDFDTDKYAAFDMWHGAESEYDDYRTVAQQSESDR
                     KLQNDEDFFCIGKHIERDDLGKQDVAKWIAETVEDLLPLYEACHGK"
     gene            complement(1976..2485)
                     /locus_tag="WIC03_000700"
     CDS             complement(1976..2485)
                     /locus_tag="WIC03_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEZ3883234.1"
                     /translation="MNPFETKSVTFAEPIEMLYACHGKVRRFCGQVAMLSDYIAENGC
                     NQIVLQTIRQITRYFNVAAPLHHEDEEEDFFPLLLQYAPQAREGVDELLRQHIGLHDN
                     WAAVSAEFAKLEADNAYVPDAEAFKRFVAGYDVHLAIEEPLFDMGNTFIPKEKLTEIG
                     EIMAARRRK"
     gene            complement(2580..3131)
                     /gene="azu"
                     /locus_tag="WIC03_000701"
     CDS             complement(2580..3131)
                     /gene="azu"
                     /locus_tag="WIC03_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="azurin"
                     /protein_id="HEZ3883235.1"
                     /translation="MKAYLALISAAVIGLAACSQEPAAPAAEATPAAEAPASEAPAAE
                     AAPADAAEAPAVGNCAATVESNDNMQFNTKDIQVSKACKEFTITLKHTGTQPKASMGH
                     NLVIAKAEDMDGVFKDGVGAADTDYVKPDDARVVAHTKLIGGGEEASLTLDPAKLADG
                     EYKFACTFPGHGALMNGKVTLVD"
     gene            complement(3412..3834)
                     /locus_tag="WIC03_000702"
     CDS             complement(3412..3834)
                     /locus_tag="WIC03_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HEZ3883236.1"
                     /translation="MNLEQLGRRDALLSGLLKQAGQWRRLDAAVKKLLPANLHPHFQT
                     VCIEDGRLVLLAANNMAASRLKMIAPSVLPQLAGLDASIRSVSVRLVPKPEKPPKTNT
                     LHLSKAALESFGSAAVKLEKRHPELAEALANLVRRHGA"
     gene            3965..6715
                     /gene="secA"
                     /locus_tag="WIC03_000703"
     CDS             3965..6715
                     /gene="secA"
                     /locus_tag="WIC03_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEZ3883237.1"
                     /translation="MLTNIAKKIFGSRNDRLLKQYRKSVARINALEEQMQALSDADLQ
                     AKTAEFKQRLADGQTLDGILPEAFAVCREASRRTLGMRHFDVQLIGGMVLHDGKIAEM
                     RTGEGKTLVATLAVYLNALAGKGVHVVTVNDYLASRDAGIMEPLYNFLGLTVGVIISD
                     MQPFDRQNAYAADITYGTNNEFGFDYLRDNMVTDQYDKVQRELNFAVVDEVDSILIDE
                     ARTPLIISGQADDNIQLYQIMNTVPPHLVRQETEEGEGDYWVDEKAHQVILSEAGHEH
                     AEQILTQMGLLAENDSLYSAANIALMHHLMAALRAHSLFHKDQHYVIQDGEIVIVDEF
                     TGRLMSGRRWSEGLHQAVEAKEGVEIKRENQTLASITFQNYFRLYTKLSGMTGTADTE
                     AFEFQSIYNLETVIIPTNRPVQRKDFNDQIFRSAEEKFEAVVKDIEECHKRGQPVLVG
                     TTSIENSELVSHLLQKAGLPHNVLNAKEHEREALIVAQAGKVGAITVATNMAGRGTDI
                     VLGGNLKHQTDAIRADETLSDEEKQAQIAALENGWQAEHDKVMEAGGLHIIGTERHES
                     RRIDNQLRGRSGRQGDPGSSRFYLSFEDPLLRLFALDRAAAILNRLAPERGVAIEHNL
                     LTRQIEGAQRKVEGRNFDMRKQVLEYDDVANEQRKVIYSQRNEILTSKDISDLMQEIR
                     SDVVSDLVDTYMPPDSMEEQWDIPTLENRLAAEFRLHEDIQSWLKADNAIDGQDIKER
                     LIERIENEYAAKTELVGKQAMADFERNVMLQVIDNQWREHLAAMDYLRQGIHLRSYAQ
                     KNPKQEYKREAFTMFQDLWNGIKFHIASLLTSVQIEQNPVAVVEEQPIGNIQSIHSES
                     PDMEELLGQSQTDLVTEAFNPDGTDFSPEALEARGQIVHRNDPCPCGSGLKYKQCHGK
                     LA"
     gene            6859..8631
                     /locus_tag="WIC03_000704"
     CDS             6859..8631
                     /locus_tag="WIC03_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEZ3883238.1"
                     /translation="MIPSDFIDELLAKTDIVDIIDEQVPLKKGGANYMACCPFHKEKT
                     PSFSVSPTKQFYHCFSCGAHGSAIGFVMEHQGLSFPEAVQFLADRVGMIVPKVRGQND
                     NPEVRAERKKKQQTLEETTAAAADFYAQQLKFNPTAKAYLDKRGLSAEVIAHYGLGYA
                     PDGWQPLAQVFQPYPNTALVDTGMVIDNEGRYYDRFRHRIMFPIRNPRGQVIGFGGRV
                     LDDSKPKYLNSPDTPLFDKGKNLYGLYEGRAAVKEVGRILVVEGYMDVVALAQFGVGY
                     GVAALGTATTAEHVKILMRQADSIYFCFDGDSAGRKAAWRALENALPQLKDDKSLHFL
                     FLPEEHDPDSYIRAYGKAQFEDALLNQSKPLSEYFWEHLSDGIHLNTQEGKAELVKTS
                     SPLLAQITAPALAYLLKQRLSELVGIDPDNLAQLLGQEAPKRHVKQKNYKLPPISVKQ
                     PVMPTLVQRQIRSLLINPDWAAYIDLPDYLALDGDFACLANLAETIKNHPSVPATAQV
                     LEHMRGSPYEETINRIFRSALQSEEMEGGGEEDCENFQIGIKKLLNELKYSQIETLKQ
                     KSLQSGLNESEKKLLLSLLTAKQN"
     gene            8818..10746
                     /gene="rpoD"
                     /locus_tag="WIC03_000705"
     CDS             8818..10746
                     /gene="rpoD"
                     /locus_tag="WIC03_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEZ3883239.1"
                     /translation="MSKNQNHEEYQDDARPLTIEEQRARLRQLIIMGKERGYITYSEI
                     NDALPDDMSDADQIDNIVSMISGLGIQVTEHAPDAEDILLSDNAAVTDDDAVEEAEAA
                     LSSADSEFGRTTDPVRMYMREMGQVDLLTREDEIIIAKKIENALKNMVQAISACPGSI
                     AEILELIEKIRKDEIRVDEVVEAIIDPNEVLLNELGLGHLETTAPEKPSNDNSDENED
                     DEESEEDADEISAANLAELKQKVIGHFAQIEKDYKKMIGRLEKHHSRHKDYLAYRDAI
                     ANKLLEVRFATRQIDSLSSSLRGKVENIRKLEREIRDICLDRVHMERDYFIQNFLPEI
                     TNLEWIEEEIAKGRVWSDALDRFRHAILEKQTELADMEKETRISIEELKEINKNMVSS
                     EKETAAAKQEMIQANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYK
                     FSTYATWWIRQAITRSIADQARTIRIPVHMIETINKMNRISRQHLQETGEEPDSAKLA
                     ELMQMPEDKIRKIMKIAKEPISMETPIGDDDDSHLGDFIEDANNVAPADAAMYTSLHE
                     VTKEILESLTPREAKVLRMRFGIDMNTDHTLEEVGRQFDVTRERIRQIEAKALRKLRH
                     PTRSDRLRSFLDSEDSKL"
BASE COUNT         2826 a         3023 c         2679 g         2301 t
ORIGIN      
        1 aatgtgttat ctcaaaccca cgttaggtct aagcaaatgg tcggacatcc gtatccggca
       61 agcaagctgc tttcagacgg catttccagc caacaaccgc gccaatatcc cctcatacac
      121 cgcagacagc ttcggaatgt cgtttagccg cacgttttcg ttgatttggt ggatggtcgc
      181 attggacggg cctaattcga tgagttcttt tgcaatggct ttgatgaagc gtccgtccga
      241 agtgccgccg gtggtggaca attcggtctc tacgccgcag atttcggcaa tggcggcgcg
      301 tgccacgtcg gtcagtttgc ccgcgtgggt caggaagggc tgccccgaac acgaccactg
      361 caaatcgtat tgcacaccgt gtttgtccaa aatggcgtgg acgcgttgtt tcagccctgc
      421 ttcggtggac tcggtggaga agcggaaatt gaatttgacg ctcagctcgc ccggaatgac
      481 attggtcgcg cctgtaccgc cgttgatatt ggaaatttga aagctggtcg gcgggaagta
      541 tttgttgcct tcgtcccaga cttcctgcgt cagctctaac aaggccgggg caaaagtatg
      601 cacgggattg attgccaaat gcggataggc aatatggcct tgcttgcctt tgacggtcag
      661 gttgcccgac agcgagccgc gccggccgtt tttaatcata tcgcccaatt tgtccacggc
      721 tgtcggttcg ccgacgatgc agtagtcgat aagctcgtcg cgcgctttca atacatcgac
      781 gactttggtc gtgccgtcca acgcgtcgcc ctcttcgtcg gaagtaatca gaagcgcaat
      841 gctgccttgg tggtcgggat gttcggcaac gaagcgttca caggcggtaa cgaagcaggc
      901 gatgctggtt ttcatgtctg ccgcgccgcg cccgtataat cttccgtcgc gctcggccgg
      961 ttcgaacggg ggcgaatccc atttttcgac aggacctgtc ggtacaacgt cggtatgccc
     1021 tgcaaaacag acgacgggag ctttcgtgcc gcgtcgcaac cagatgtttt tggtgtcgcc
     1081 gaaatggagt tcttcagccg caaaaccgat tttgtgcagg cgttcggcaa ggagtttttg
     1141 gcaatccctg tcgtcagggg taacggatgg tcgggaaatc agctctttgg caagctctag
     1201 ggattgagtt tcggtcatat ttgttcactt ttgaaattag accgtctgaa acgttctgaa
     1261 tgtgattttc agacggcatt taggttaggt tggcatacgg ggtgggtatt ttacccatca
     1321 gtcttctgaa tcatttgccg tggcaggctt cgtaaagcgg cagcaaatct tccaccgttt
     1381 ccgctatcca tttcgcgaca tcctgcttgc ccaaatcgtc gcgctcgatg tgtttgccga
     1441 tgcagaaaaa gtcttcgtcg ttttgcaact ttcggtcgga ttcgctttgt tgtgcgacgg
     1501 tgcggtagtc gtcatattcg ctttccgcgc cgtgccacat atcgaatgcg gcgtatttgt
     1561 cggtatcgaa atcatccaac cagcggttgt attcgggcag cgcaatcggc gacacgtcgg
     1621 ctttgtagca gtgccaatcc aagctgacgc tcaggcggcg gcggttcaat aaaatcgata
     1681 aaatcgcggc ggaattttta tattgttcgt atttgaagta ggcaaagaaa tgggcgcgca
     1741 cctgccagcc gttacaccag cgttcgatat gcggcggcgc gaacggcgca cccaattcgg
     1801 cggcaacctg ctggatcagc tgctgccata cctgccagtt ttctttatag tctgccttga
     1861 tttgcggaat gctttcaggc tggtattttt taagctggga aaattggaaa aacgggatat
     1921 tgaacaaatc gcaacttttc gaggtaagca tagtgtattc cttgagacga ttaccttatt
     1981 tgcgccgacg cgccgccatg atttcaccga tttcggtcag tttttcttta gggataaagg
     2041 tgttgcccat atcaaacagc ggctcttcaa tcgccaaatg aacatcatat cccgccacaa
     2101 aacgtttgaa cgcttccgca tcgggaacat aagcattgtc tgcttcgagc ttggcaaatt
     2161 cggcggaaac agccgcccag ttgtcgtgca gcccgatatg ttggcgtaaa agctcgtcca
     2221 caccttcacg tgcttgcggc gcgtattgca gcagcagcgg gaaaaagtct tcttcttcgt
     2281 cctcatgatg cagcggcgcg gcaacgttga aataccgggt gatttggcgg atggtttgca
     2341 aaacaatttg attgcagccg ttttcggcga tgtagtccga cagcatggcg acttgtccgc
     2401 aaaaacggcg cactttgccg tggcaggcat acagcatttc aatcggttcg gcaaaggtaa
     2461 cgcttttggt ttcaaacgga ttcatgtttt cgttctcaac gggcactttt caagcagtca
     2521 ttttataata aaacagcctg cacaaagcag gctgtccgtc ttttgagact ttaagcggat
     2581 taatcgacca aagtcacttt gccgttcatc aaagcaccgt gacctgggaa ggtacaagcg
     2641 aatttatatt cgccgtcggc caatttagca ggatccagag tcagggaagc ttcttcgccg
     2701 ccgccgatca gtttggtatg ggcaacaacg cgtgcatcat caggtttgac atagtcggta
     2761 tcggcagcac ctacgccgtc tttaaatacg ccgtccatgt cttcagcttt ggcaatcacg
     2821 agattgtgac ccatgctggc tttgggttgc gtaccggtat gtttcagagt gatggtaaac
     2881 tctttacatg ctttgctgac ttggatgtct ttggtgttga actgcatatt gtcgttggat
     2941 tcgacagttg ccgcacagtt gccgacagca ggggcttcgg cagcatctgc aggagcagct
     3001 tcggcggcag gtgcttcgga agcgggtgct tcagcagcag gagttgcctc ggcagcaggc
     3061 gcggcaggtt cttgagagca ggcagccaaa ccgataacgg cagcagaaat cagagccaga
     3121 tacgctttca taacaaatct ccaatcgata aaataatatt cggttttaca gaaatcaaag
     3181 tgcaaccgcc attaacaaaa ccttgaaaaa gattccgccg cgttgcacaa acagatgttt
     3241 cggagcggca ttttgctaca aatttcattt gaaatcaaag cctgtctgca agtttacaat
     3301 cgtttacttt aaaaggggca attttacccc gaacctattt ctttagtatt agacccatta
     3361 tcctttactt cttaatatta acggatgttt acacaaattc ccgtatacat tttatgcgcc
     3421 atgccttcta accaagtttg ccaatgcctc cgccaattcg ggatgccgtt tttccaactt
     3481 taccgccgcc gaaccgaaac tctccagcgc agccttactc aaatgcaggg tattggtttt
     3541 cggcggtttt tccggtttcg ggaccagcct gaccgaaaca gagcgtatcg aagcgtcaag
     3601 ccctgccaac tgcggcaata ccgacggtgc aatcattttc aagcgcgatg ccgccatatt
     3661 gtttgccgcc aaaaggacaa gcctgccgtc ttcgatacat accgtctgaa aatgcgggtg
     3721 caggttggca ggcagcagtt ttttcacggc ggcatccaac cgccgccact gtcccgcctg
     3781 tttcaaaagt ccggaaagca gcgcgtcccg cctgcccaac tgttccaaat tcataaaaca
     3841 tacacccaaa aagattgaaa taccgcaaac gcgcctttat ttcagacggc attagcactt
     3901 tgcacaaacg cttgtgttaa aatcgcgttt tcgcccacta ttatatcagg cgcaggaatt
     3961 attcatgctg acaaacattg ccaagaaaat cttcggcagc cgcaacgacc gcttgctgaa
     4021 acaataccgt aaatccgttg ccagaatcaa cgcgctcgaa gaacagatgc aagccctaag
     4081 cgatgctgat ctgcaagcca aaactgccga attcaaacaa cgcctcgccg acggtcagac
     4141 tttggacggc attttgcccg aagccttcgc cgtctgccgc gaagcgtccc gccgcaccct
     4201 cggtatgcgc cacttcgacg tgcagcttat cggcggtatg gtgctgcacg acggcaaaat
     4261 cgccgaaatg cgtaccggcg aaggcaaaac cctggtcgcc accctcgccg tctatctcaa
     4321 cgcgctggcc ggcaaaggcg tacacgtcgt taccgtcaac gactacctcg cctcacgcga
     4381 tgcgggcatt atggagccgc tctacaattt cctcggcctt accgtgggcg tgattatttc
     4441 agatatgcag ccgttcgacc gtcaaaacgc ctatgccgcc gatatcacct acggcaccaa
     4501 taatgaattc ggcttcgact acctgcgcga caatatggtt accgaccaat acgacaaagt
     4561 gcagcgcgaa ttgaattttg ccgttgtcga tgaagtggat tccatcttga ttgacgaagc
     4621 gcgcactccg ctgattatct ccggtcaggc ggatgacaac atccagttgt accaaatcat
     4681 gaacaccgtt ccgccccacc tcgtccgtca agagacagaa gaaggcgaag gcgactattg
     4741 ggtcgacgaa aaggcacatc aggtcatcct gagcgaagca ggtcacgaac acgccgagca
     4801 aatcctgacc caaatgggat tgctggcaga aaacgactcc ctctattccg ccgccaatat
     4861 cgccctgatg caccacctta tggcggcatt gcgcgcgcat tccctcttcc acaaagacca
     4921 acattacgtc atccaagacg gcgaaatcgt catcgtggac gaattcaccg gccggctgat
     4981 gtccggccgc cgctggtcgg agggtctgca tcaagccgtc gaagccaaag aaggcgtgga
     5041 aatcaaacgc gaaaaccaaa cccttgcctc catcaccttc caaaactatt tccgcctgta
     5101 caccaagctc tccggcatga ccggcacagc cgataccgaa gctttcgagt tccaaagcat
     5161 ctacaacctc gaaaccgtca tcatcccgac caaccgccct gtacagcgca aagacttcaa
     5221 cgaccagatt ttccgttccg ccgaagaaaa attcgaagcc gtcgtcaaag acatcgaaga
     5281 atgccacaaa cgcgggcagc ccgtcctcgt cggcaccacc agcatcgaaa actccgaact
     5341 ggtctcccac cttctgcaaa aagccggact gccgcacaac gtcctcaacg ccaaagaaca
     5401 cgaacgcgaa gccctgattg tcgcccaagc cggcaaagtc ggcgcgatta ccgttgccac
     5461 caatatggcg ggacgcggta cggacatcgt tttaggcggc aacctgaagc accaaaccga
     5521 tgccatccgt gccgacgaaa ccttgagcga cgaagagaaa caggcacaaa tcgccgcact
     5581 cgaaaacggc tggcaggcag aacacgataa agtaatggaa gcaggcggcc tgcacatcat
     5641 cggtacggaa cgccacgaaa gccgccgtat cgacaaccaa ttgcgcggac gttccggccg
     5701 tcagggcgac cccggatcca gccgtttcta cctttcgttt gaagacccgc tgctgcgctt
     5761 attcgcactc gaccgcgccg ccgccatcct caaccgtctc gcccccgaac gcggcgtcgc
     5821 catcgaacac aacctgctga cgcgccaaat cgaaggggcg caacgcaaag tcgaaggcag
     5881 aaacttcgat atgcgcaaac aggttttgga atacgacgac gttgccaacg aacagcgcaa
     5941 agtcatttac agccagcgca acgaaattct gaccagcaaa gacatcagcg acctgatgca
     6001 ggaaatccgt tctgatgtcg tcagcgacct cgtggatacc tatatgccgc ccgacagcat
     6061 ggaagaacaa tgggacatcc cgactttgga gaaccgtctg gctgccgaat tcagactgca
     6121 cgaagacatc caatcctggc tgaaggcgga caatgcgatt gacggtcaag acatcaaaga
     6181 acgcctgatc gaacgcatcg aaaacgaata tgccgccaaa accgaactgg tcggcaagca
     6241 ggcaatggcc gatttcgagc gcaacgtgat gttgcaggtc atcgacaacc aatggcgcga
     6301 acacctcgcc gctatggact acctgcgaca aggcatacac ctgcgcagct atgcccaaaa
     6361 aaatccgaag caggaatata aacgtgaagc ctttaccatg ttccaagacc tgtggaacgg
     6421 catcaaattc catattgcct ccctgcttac ctcggttcaa atcgaacaaa accctgtcgc
     6481 ggtggttgaa gagcaaccca tcggcaacat ccagtccatc cattccgaat cgcccgatat
     6541 ggaagaactt ttgggtcagt cgcaaaccga tttggttacc gaggccttta atcccgatgg
     6601 aacggatttc agccccgaag ccttggaagc gcggggacaa atcgtccacc gcaacgaccc
     6661 ctgcccctgc ggcagcggtt tgaaatacaa acaatgccac ggcaaactgg cttgagcgtt
     6721 tgaacgcaaa tgccgtctga acatccggtt tccggcttca gacggcattt tgcctgaacc
     6781 gccacatccg accgccattc cgaaaaatcc cgatttcgta ccgtccaaac taaaaacagg
     6841 catccgcccc gccgaactat gattccatcc gacttcattg acgaacttct ggccaaaacc
     6901 gatattgtcg acatcatcga cgaacaggtt ccgctgaaaa aaggcggggc gaactatatg
     6961 gcgtgttgcc cgttccacaa ggaaaaaacg ccgtcgtttt cggtcagtcc gaccaagcag
     7021 ttttaccact gtttcagttg cggggcgcac ggttcggcga ttggttttgt gatggaacat
     7081 caggggctgt cgtttccgga ggcagtgcag ttccttgccg accgcgtggg tatgattgtg
     7141 cctaaagtgc gcgggcagaa cgataatccc gaagtccgtg ccgaacgtaa gaaaaagcag
     7201 cagacgctgg aagaaacgac agcggcggcg gctgattttt atgcgcaaca gttgaaattc
     7261 aatccgacgg cgaaggctta tttggacaag cgcggcttga gcgcggaagt catcgcgcat
     7321 tatggtttgg gctacgcgcc cgacggctgg cagcctttgg cgcaagtatt ccaaccgtac
     7381 ccgaataccg cgttggtgga tacgggtatg gtgattgaca atgaggggcg gtattacgac
     7441 cgattccgtc atcggattat gttccccatc cgcaatccgc gcgggcaggt catcggtttc
     7501 ggcggcaggg tgctggacga ctccaaaccc aaatatctaa actctcccga tacgcctttg
     7561 tttgataagg ggaaaaacct ttacggcttg tatgaagggc gtgctgctgt caaggaagtg
     7621 gggcggattc tggtggtcga aggctatatg gacgtggtcg cgctggcaca gttcggcgtg
     7681 ggctacggcg tggcggcctt gggcacggca acaacagcgg aacacgtcaa aatcctgatg
     7741 cgtcaggcgg acagtattta tttctgtttc gacggcgaca gcgcggggcg caaagcggct
     7801 tggcgcgcgc tggaaaacgc gctgccgcag ttgaaggacg acaaatcgct gcattttttg
     7861 ttcctgccgg aagaacacga ccccgacagt tacatccgtg cctacggcaa agcgcaattt
     7921 gaagacgcgc ttctgaatca aagcaagcct ttgtcggagt atttctggga acacctttca
     7981 gacggcattc atctcaatac gcaggaaggc aaggcggaat tggtgaaaac cagttcgccg
     8041 cttttggcgc agattaccgc gccggcattg gcttatttgt taaaacaacg gcttagcgaa
     8101 ctggtcggca tcgaccccga caacctcgcg caactgctgg gacaggaagc gccgaagcgg
     8161 cacgtcaaac aaaaaaacta caaactgccc ccgatttccg tcaaacagcc cgttatgccg
     8221 acattggtgc aacggcaaat ccgcagcctc ttgataaatc cggattgggc tgcatatata
     8281 gacctgcccg attatctggc gttggacggc gatttcgcct gccttgccaa cctcgccgaa
     8341 accattaaaa accatccttc cgtgccggca accgcacagg ttttggaaca tatgcgcggt
     8401 tcgccctacg aagaaacaat caaccgcatc ttccggtcgg cccttcaatc ggaagaaatg
     8461 gaaggcggcg gcgaagaaga ttgcgaaaac ttccaaatcg gcatcaaaaa actgctcaat
     8521 gagttaaaat acagccaaat cgaaacatta aaacaaaaaa gcctgcaatc cggcttaaat
     8581 gaaagcgaga aaaaactttt gctgtcgctg ctgaccgcaa aacaaaattg accggcggat
     8641 tccgccatcc gtaaaccgtt atgccgtctg aaaagcattc accccggctg caacaacgac
     8701 acctgcagaa cacccatccc caaaagcctt cagacggcat cagagtaccc tactctgcca
     8761 cgccttcagg tgcgtccaaa cgcaaaccgt cggcatcttg ccaacagaaa gcagacaatg
     8821 tccaaaaacc aaaatcacga agaataccaa gacgatgccc gccccctgac gattgaggaa
     8881 caacgcgcgc gcctgcgcca actcatcatt atgggcaaag aacgcggcta catcacctac
     8941 tccgaaatca atgacgcact gcccgacgat atgtccgatg ccgatcaaat cgacaacatc
     9001 gtcagtatga tttccggttt gggtatccaa gttaccgaac acgcccccga tgcggaagac
     9061 atattgttaa gcgacaatgc cgccgttacc gacgatgatg ccgtcgaaga agccgaggcc
     9121 gccctttcca gcgccgactc cgagttcggc aggacgaccg accccgtccg tatgtatatg
     9181 cgcgaaatgg ggcaggtcga cctgctgacc cgcgaagacg aaatcatcat cgccaaaaaa
     9241 atcgaaaacg ccctgaaaaa tatggttcag gccatctccg cctgcccggg atccattgct
     9301 gaaatcttag aactcatcga aaaaatccgc aaagacgaaa tccgcgtcga cgaagtcgta
     9361 gaagccatta tcgacccgaa tgaagtattg ctcaacgaat tgggcttggg gcacttggaa
     9421 accacagcac ccgagaaacc ttccaacgac aattcggacg aaaacgaaga cgacgaagaa
     9481 tcggaagaag atgcggatga aatctcggca gccaatctcg ccgaattgaa acaaaaagtc
     9541 atcggccact ttgcccaaat cgaaaaagac tacaaaaaaa tgatcggccg tttggaaaaa
     9601 caccacagcc ggcacaaaga ctatctcgcc taccgcgacg cgattgccaa caaactgctg
     9661 gaagtccgtt tcgccacccg gcaaatcgac agcctcagca gcagcctgcg cggaaaagta
     9721 gaaaacatcc gcaaactcga acgcgaaatc cgcgacatct gcctcgaccg cgtccatatg
     9781 gagcgcgact acttcatcca aaacttcctg cccgaaatca ccaatctgga atggattgaa
     9841 gaagaaatcg ccaaaggcag ggtttggagc gacgcgctcg accgcttccg ccacgccatc
     9901 ctcgaaaaac aaaccgagtt ggcggatatg gaaaaagaaa cccgcatttc catcgaagag
     9961 ttgaaagaaa tcaacaaaaa tatggtgtcg agcgaaaaag aaaccgcagc cgccaaacag
    10021 gaaatgattc aggcaaactt gcgcctcgtg atttccatcg ccaaaaaata taccaaccgg
    10081 ggcttacaat tccttgatct gattcaggaa ggcaacatcg gtttgatgaa agcggtcgat
    10141 aagttcgaat accgcagagg ctataaattc tccacctacg caacctggtg gatccgccag
    10201 gcaatcaccc gctcgattgc cgatcaagcg cgtaccatcc gcattccggt acatatgatt
    10261 gaaaccatca acaagatgaa ccgcatctcg cgccaacacc ttcaagaaac cggcgaagaa
    10321 cccgattccg ccaaacttgc cgaactgatg cagatgcccg aagacaaaat ccgcaaaatc
    10381 atgaaaatcg ccaaagagcc gatttcgatg gaaaccccca tcggcgacga cgacgattcg
    10441 cacttgggcg acttcatcga agatgccaac aatgttgcgc cggccgatgc ggcaatgtac
    10501 accagcctgc acgaagtaac caaagaaatc ctcgaaagcc tgacaccgcg tgaggcaaaa
    10561 gtcctgcgta tgcgtttcgg catcgatatg aacaccgacc acacgctgga agaagtcggc
    10621 agacagtttg acgtaacgcg cgaacgcatc cgccaaattg aagcaaaggc actccgcaaa
    10681 ttacgccatc cgacaagaag cgaccggctg agaagtttct tggacagcga agacagcaaa
    10741 ctataaccct caaaaaaacc gcaggtttca aatacctgcg gttttttctt acacaataaa
    10801 caacgcttcc acatatccca cactcctat
//
LOCUS       DAWHSK010000040        10518 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.040, whole genome shotgun sequence.
ACCESSION   DAWHSK010000040 DAWHSK010000000
VERSION     DAWHSK010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10518
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.040"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(191..2599)
                     /locus_tag="WIC03_000706"
     CDS             complement(191..2599)
                     /locus_tag="WIC03_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HEZ3883240.1"
                     /translation="MARLFSLKPLVLALGLCFGTHCAAADAVAAEETDNPTAGGSVRS
                     VSEPIQPTSLSLGSTCLFCSNESGSPERTEAAVRGSGEASIPEDYTRIVADKVEGQSQ
                     VQVRAEGNVVVERNRTTLNTDWADYDQSGDTVTAGDRFALQQDGTLIRGETLTYNLEQ
                     QTGEAHNVRMEIEQGGRRLQSVSRTAEMLGEGHYKLTETQFNTCSAGDAGWYVKAASV
                     EADREKGIGVAKHAAFVFGGVPLFYTPWADFPLDGNRKSGLLVPSLSAGSDGVSLSVP
                     YYFNLAPNLDATFAPGVIGERGAVFDGQVRYLRPDYAGQSDLTWLPHDKKSGRNNRYQ
                     AKWQHRHDISDTLQAGVDFNQVSDSGYYRDFYGNKEIAGNVNLNRRVWLDYGGRAAGG
                     SLNAGLSVLKYQTLANQSGYKDKPYALMPRLSADWRKNTGRAQIGVSAQFTRFSHDSR
                     QDGSRLVVYPDIKWDFSNSWGYVRPKLGLHATYYSLNRFGSQEARRVSRTLPIVNIDS
                     GATFERNTRMFGGGVLQTLEPRLFYNYIPAKSQNDLPNFDSSESSFGYGQLFRENLYY
                     GNDRINTANSLSAAVQSRILDGATGEERFRAGIGQKFYFKDDAVMLDGSVGKKPRNRS
                     DWVAFASGSIGSRFILDSSIHYNQNDKRAENYAVGASYRPAQGKVLNARYKYGRNEKI
                     YLKSDGSYFYDKLSQLDLSAQWPLTRNLSAVVRYNYGFEAKKPIEVLAGAEYKSSCGC
                     WGAGVYAQRYVTGENTYKNAVFFSLQLKDLSSVGRNPADRMDVAVPGYIPAHSLSAGR
                     NKRP"
     gene            2669..3673
                     /locus_tag="WIC03_000707"
     CDS             2669..3673
                     /locus_tag="WIC03_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEZ3883241.1"
                     /translation="MQRQTELKNWLQTVYPERDFDLTFAAADADFRRYFRATFSDGSS
                     VVCMDAPPDKMSVAPYLKVQKLFDMVNVPQVLHADTDLGFVVLNDFGNTTFLTAMLQE
                     QGETAHKALLLEAIGELVELQKASREGVLPEYDRETMLREINLFPEWFVAKELGRELT
                     FKQRQLWQQTVDTLLPPLLAQPKVYVHRDFIVRNLMLTRGRPGVLDFQDALYGPISYD
                     LVSLLRDAFIEWEEEFVLDLVIRYWEKARAAGLPVPEAFDEFYRWFEWMGVQRHLKVA
                     GIFARLYYRDGKDKYRPEIPRFLNYLRRVSRRYAELAPLYALLVELVGDEELETGFTF
                     "
     gene            complement(3730..4428)
                     /locus_tag="WIC03_000708"
     CDS             complement(3730..4428)
                     /locus_tag="WIC03_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEZ3883242.1"
                     /translation="MKSRQLALGVAALFALAACDSKVQTSVPADSAPAASAAAAPAGL
                     VEGQNYTVLANPIPQQQAGKVEVLEFFGYFCPHCAHLEPVLSKHAKSFKDDMYLRTEH
                     VVWQKEMLPLARLAAAVDMAAADSKDVANSHIFDAMVNQKIKLQEPEVLKKWLGEQTA
                     FDGKKVLAAYESPESQARADKMQELTETFQIDGTPTVIVGGKYKVEFADWESGMNTID
                     LLADKVREEQKAAQ"
     gene            4455..4646
                     /locus_tag="WIC03_000709"
     CDS             4455..4646
                     /locus_tag="WIC03_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883243.1"
                     /translation="MLEINRNPAILLHFRVLIQFAAGKGRFRFGKPLQTASNPMPSEA
                     VSVVQYAPLPQTGTTVEEQ"
     gene            4646..6184
                     /gene="murJ"
                     /locus_tag="WIC03_000710"
     CDS             4646..6184
                     /gene="murJ"
                     /locus_tag="WIC03_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEZ3883244.1"
                     /translation="MNMLGALAKVGSLTMVSRVLGFVRDTVIARAFGAGMATDAFFVA
                     FKLPNLLRRVFAEGAFAQAFVPILAEYKETRSKEAAEAFIRHVAGMLSFVLVIVTALG
                     ILAAPWVIYVSAPGFAQDADKFQLSIDLLRITFPYILLISLSSFVGSVLNSYHKFGIP
                     AFTPTFLNVSFIVFALFFVPYFDPPVTALAWAVFVGGILQLGFQLPWLAKLGFLKLPK
                     LNFKDAAVNRVMKQMAPAILGVSVAQVSLVINTIFASYLQSGSVSWMYYADRMMELPG
                     GVLGAALGTILLPTLSKHSANQDTEQFSALLDWGLRLCMLLTLPAAVGLAVLSFPLVA
                     TLFMYREFTLFDAQMTQHALIAYSFGLIGLIMIKVLAPGFYARQNIKTPVKIAIFTLI
                     CTQLMNLAFIGPLKHVGLSLAIGLGACINAGLLFYLLRKHGIYRPGKGWAAFLAKMLL
                     ALAVMGGGLYAAQIWLPFDWTHTGGMQKAARLFILIVIGGCLYFASLAALGFRPRHFK
                     RVES"
     gene            6317..7273
                     /locus_tag="WIC03_000711"
     CDS             6317..7273
                     /locus_tag="WIC03_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheT family hydrolase"
                     /protein_id="HEZ3883245.1"
                     /translation="MILTPPDTPLFLRNGNADTIAAKFLQRPAPAYRRELLPDSTGKT
                     KVAYDFSDGISPDAPLVVLFHGLEGSSRSHYAVELMLAVRDRGWHGVVVHFRSCGGIA
                     NTAPVFYHLGDTAEIAFTLDTFAARYREIYAVGVSLGGNALAKYLGEQGKKALPQAAA
                     VISAPVDAEAAGRRFDSGITRLLYTRYFLRTLIPKAKSLQGFQTAFAAGCKTLGEFDD
                     RFTAPLHGFADRHDYYRQTSCKPLLKHVAKPLLLLNAVNDPFLPPEALPIPDEVSEAV
                     TLFQPAHGGHVGFVGSTGGRLHLQWLPQTVLSYFDSFRTNSR"
     gene            7323..8105
                     /gene="trpC"
                     /locus_tag="WIC03_000712"
     CDS             7323..8105
                     /gene="trpC"
                     /locus_tag="WIC03_000712"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEZ3883246.1"
                     /translation="MTDILNKILATKAQEVAAQKAAVNAEHIRALAAEAAPVRSFIDS
                     IRGKHRLNLPAVIAEIKKASPSKGLIRPDFRPAEIARAYENAGAACLSVLTDEPYFQG
                     SPEYLKQAREAVSLPVLRKDFIIDEYQVYQARAWGADTVLLIAAALEQEQLERFEAVA
                     HELGMTVLLELHDETELEKCRNLTTPLWGVNNRNLRTFEVSLDQTLSLLPALEGKTVV
                     TESGITGKADVEFMQSRGVHTFLIGETFMRAGDIEAEVGKLF"
     gene            8170..10470
                     /gene="recQ"
                     /locus_tag="WIC03_000713"
     CDS             8170..10470
                     /gene="recQ"
                     /locus_tag="WIC03_000713"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEZ3883247.1"
                     /translation="MHRPTAKQILHEVFGYPEFRGRQEAVINTLAGGGSLTVLMPTGG
                     GKSLCYQIPALMREGVAVVVSPLIALMNDQVANLHAAGIEAAAVNSSTSADGAREIAD
                     KLAQGRLKLLYVAPERLVTDRFLRFLDQQTVSLFAIDEAHCVSQWGHDFRPEYQQLGM
                     LAERYPNVPRIALTATADAATRADIKHYLHLDDAPEFISSFDRPNIYYQVIEKNNGKK
                     QLLEFIRKEMTGQSGIVYCLSRKKVEDVAQFLRENGLNAIPYHAGLSMDVREENQRRF
                     TREDNIIVVATVAFGMGIDKPDVRFVAHLDMPQSVEHFYQESGRAGRDGLPAVSWLCY
                     GLNDWVLLHERIAEGNSDEVQKQIEMQKLDAMLAVCETAACRRVLLLKHFGEASEPCG
                     HCDNCLHPPVRFDGTVLVQKLLSCVYRAGQRFAAGYITNLLRGKSDDWIRGNRHEQLS
                     TFGIGTELSNKEWRSVIRQCISLGYLTVNITRYQALQLTEAAKKVLKGETEVMLRPLK
                     RDKPAARTLKDNWLRTEREERLWQALRVWRMKQAEAEGIPAYMIFGDKTLRDLVEKMP
                     QDLNGLHDIYGLGEAKIDRFGHGILEVCRNAADFSHDAVIRPQTEREQQLRQKLEAWR
                     YEQARAENCALHAVLSDESLADMLAATPETETDLEGVHGLGSVRAAKYGRDILAVCRP
                     FSDGIDEAAKHKRRLMRALIQWCGETAKHEQSEPYRILSKAALRAIAAKQPEGLAELA
                     AVYGVGEEKAARYGAAVLAVLERNAV"
BASE COUNT         2158 a         2984 c         3012 g         2364 t
ORIGIN      
        1 gatgcggttt ttgccttttg cggtgcggcg tggacatcgg ctgctgccag caatgcggcg
       61 gcaatcatca gggctttgat tttcatcatt ctttccttat cgtttgaatc ctacctttga
      121 tggcgggttt ggggttgtcg gtggaatgcc gtcttgtgcg ggaacggtgc tcctgccggg
      181 tttccgacgg tcagggtcgt ttgttgcgtc cggcggaaag agagtgggcg gggatgtagc
      241 cgggaacggc gacatccatc ctgtctgcgg ggtttctgcc gacactgctg aggtctttca
      301 actgaagtga gaaaaagaca gcgtttttgt aggtgttttc gccggtaacg tagcgttggg
      361 cgtacacgcc cgcgccccag cagccgcaac tgcttttgta ttccgcaccc gccagcacct
      421 ctatcggttt tttggcttca aaaccgtagt tgtaacggac gacggccgac aggttgcgcg
      481 tcagcggcca ttgtgcggac aggtcgagct ggctgagttt gtcgtaaaaa taggaaccgt
      541 cggacttcag gtagattttt tcgttgcgcc cgtatttgta gcgggcgttc agcactttgc
      601 cctgtgcggg acggtagctt gcaccgacgg cgtagttctc ggcgcgtttg tcgttttggt
      661 tgtagtggat gctgctgtcg aggatgaagc ggctgccgat gctgccggag gcaaatgcca
      721 cccagtcgga acggttgcgc ggttttttgc cgacgctgcc gtcaagcatc accgcatcat
      781 ccttgaaata gaatttctga ccgatgccgg cgcggaaacg ctcttccccc gtcgcgccgt
      841 ccaaaatacg gctttgcacg gcggcggaaa ggctgtttgc ggtgttaatc ctgtcgttgc
      901 cgtaatagag gttttcgcga aagagctgcc cgtagccgaa gctgctttcc gacgaatcga
      961 aattgggcag gtcgttttgg gatttggcag gaatatagtt gtagaacagg cgcggctcga
     1021 gggtttgcag gactccgccg ccgaacatcc gcgtattccg ctcaaaagtt gcgccgctgt
     1081 cgatgttgac aatgggcaga gtgcggctga cgcgtcgggc ttcttggctg ccgaagcggt
     1141 tgaggctgta ataggtggcg tgcagtccga gtttgggacg gacgtaaccc cagctgttgc
     1201 tgaaatccca tttgatgtcg ggatagacga cgaggcggct gccgtcttgg cggctgtcgt
     1261 ggctgaagcg ggtaaattgt gcggacacgc cgatttgcgc cctgccggtg tttttgcgcc
     1321 aatcggcgga aaggcgcggc atcagggcat acggtttgtc tttgtagccg ctttggtttg
     1381 ccagcgtctg gtatttcaga accgaaaggc cggcattcag gctgccgccc gccgccctgc
     1441 cgccataatc cagccataca cggcggttga ggttgacgtt gccggcgatt tctttgttgc
     1501 cgtaaaagtc gcggtagtag ccgctgtcgg agacttggtt gaaatcgaca cccgcctgaa
     1561 gcgtgtcgga aatgtcgtgc cgatgctgcc atttcgcctg atagcggtta ttcctgccgc
     1621 tttttttgtc gtgcggcagc caggtcaggt cggactggcc ggcataatcc ggccgcaggt
     1681 agcgtacctg cccgtcaaag accgcgccgc gttcgccgat cacgccgggc gcgaacgtgg
     1741 catcgagatt gggggcaagg ttgaaataat agggaacgga aagggaaacg ccgtccgaac
     1801 cggcggacag tgagggaaca agcaggccgc ttttgcggtt gccgtcaagc gggaagtccg
     1861 cccaaggcgt atagaaaagg ggaacgccgc cgaacacgaa ggcggcgtgt ttggcaacgc
     1921 ctatgccttt ttcccgatcg gcttcgacag aggctgcctt gacataccag ccggcatcgc
     1981 cggcggaaca ggtgttgaat tgggtttccg tcagtttgta atgcccttcg cccaacattt
     2041 cggcggtgcg gctgacgctt tgcagccgcc gtccgccttg ttcgatttcc atgcggacgt
     2101 tgtgcgcttc gccggtctgc tgctcgagat tgtaggtcag ggtttcgccc cgaatcagcg
     2161 taccgtcctg ttggagggcg aaccggtcgc ctgcggtaac ggtgtcgccc gactggtcgt
     2221 aatccgccca atcggtattg agggtcgtcc ggttgcgttc gacgacgacg ttgccttcgg
     2281 cacgcacctg cacctgcgac tgtccttcca ccttgtcggc aacaatgcgc gtatagtctt
     2341 cggggatgga tgcttcgccg ctgcctcgga cggcggcttc ggttctctcg gggctgccgc
     2401 tttcgttact gcaaaacagg caggtcgaac cgaggctcag gctggtaggc tgtatgggtt
     2461 cggacacgct ccgaacgctt cctccggcgg tcggattgtc cgtttcctcc gccgcaacgg
     2521 catcggcggc ggcgcaatgc gtgccgaagc agaggcccaa tgccagcacc agtggtttga
     2581 gtgaaaataa acgagccaaa atcgcccctc aagtcggttt gccagttaga atagcgttta
     2641 ttgtaacctg aaatgcttta gtactgttat gcaacggcaa accgaactga aaaattggct
     2701 tcagaccgtt tatcccgaac gggacttcga tctgactttt gcggcggcgg atgctgattt
     2761 ccgccgctat ttccgtgcaa cgttttcaga cggcagcagt gtcgtctgca tggatgcacc
     2821 gcccgacaag atgagtgtcg caccttattt gaaagtgcag aaactgtttg acatggtcaa
     2881 tgtgccgcag gtattgcacg cggacacgga tctggggttt gtggtattga acgactttgg
     2941 caatacgacg tttttgaccg caatgcttca ggaacagggc gaaacggcgc acaaagccct
     3001 gcttttggag gcaatcggcg agttggtcga attgcagaag gcgagccgtg aaggggtttt
     3061 gcccgaatat gaccgtgaaa cgatgttgcg cgaaatcaac ctgttcccgg aatggtttgt
     3121 cgcaaaagaa ttggggcgcg aattaacatt caaacaacgc caactttggc agcaaaccgt
     3181 cgatacgctg ctgccgcccc tgttggcgca gcccaaagtc tatgtgcacc gcgactttat
     3241 cgtccgcaac ctgatgctga cgcgcggcag gccgggcgtt ttagacttcc aagacgcgct
     3301 ttacggcccg atttcctacg atttggtgtc gctgttgcgc gatgccttta tcgaatggga
     3361 agaagaattt gtcttggact tggttatccg ctactgggaa aaggcgcggg ctgccggcct
     3421 gcccgtcccc gaagcgtttg acgagtttta ccgctggttc gaatggatgg gcgtgcagcg
     3481 gcacttgaag gttgcaggca tcttcgcacg cctgtactac cgcgacggca aagacaaata
     3541 ccgtcccgaa atcccgcgtt tcctcaatta cctgcgccgc gtatcgcgcc gttatgccga
     3601 actcgccccg ctctacgcgc tcttggtcga actggtcggc gatgaagaac tggaaacggg
     3661 ctttacgttt taaacccaat caaaatgccg tctgaaaacc aagtttcaga cggcattttt
     3721 caaacgggct tactgcgcgg ctttttgttc ttcgcgtact ttgtccgcca aaaggtcgat
     3781 ggtgttcata ccggactccc agtcggcaaa ttcaacttta tatttaccgc cgacgataac
     3841 cgtgggcgta ccgtcgattt ggaaggtttc ggtcagctcc tgcattttgt cggcgcgcgc
     3901 ctggctttcg ggggactcgt aggcggcaag gacttttttg ccgtcaaagg cggtttgttc
     3961 gcccagccat tttttgagga cttccggctc ttgcagcttg attttttggt tgaccatcgc
     4021 atcgaaaata tggctgttcg ccacatcttt gctgtcggcg gcagccatat cgacggcggc
     4081 ggcgaggcgt gccagcggca gcatttcttt ttgccaaacg acatgctcgg tacgcaggta
     4141 catatcgtct ttaaaagact tggcgtgttt gctcaaaaca ggttcgaggt gggcgcagtg
     4201 cggacagaaa tagccgaaaa actcaaggac ttcgactttg cctgcctgct gttggggaat
     4261 cgggttggca aggacggtat agttttgccc ttcgaccagc cctgccgggg cggcggctgc
     4321 cgaagcggca ggcgcgctgt cggcggggac gctggtttgg actttgctgt cgcacgcggc
     4381 aagggcgaac agggcggcaa cgccgagggc gagttgtctg gatttcatac ggctctccgt
     4441 gatgttggaa acagatgttg gaaatcaatc ggaatccggc tattttattg catttccgcg
     4501 tgttgataca gtttgccgcc ggaaaaggac gttttcgttt cgggaaaccg cttcagacgg
     4561 catcaaaccc gatgccgtct gaagcggttt ctgtcgtaca atacgcgccg ttgccccaaa
     4621 cgggtacgac tgttgaggaa caatgatgaa tatgctggga gctttggcaa aagtcggcag
     4681 cctgacgatg gtgtcgcgcg ttttgggatt tgtgcgcgat acggtcattg cgcgggcatt
     4741 cggcgcgggt atggcgacgg atgcgttttt tgtcgcgttc aaactgccca acctgctgcg
     4801 ccgcgtgttt gcggaggggg cgtttgccca agcgtttgtg ccgattttgg cggaatataa
     4861 ggaaacgcgt tctaaagagg cggcggaggc ttttatccgc catgtggcgg ggatgctgtc
     4921 gtttgtgctg gtcatcgtta ccgcgctggg catactcgcc gcgccttggg tgatttatgt
     4981 ttccgcaccc ggctttgccc aagacgcgga caagttccaa ctttccatcg atttgctgcg
     5041 gattacgttt ccttatatcc tattgatttc actttcctct tttgtcggct cggtactcaa
     5101 ttcctatcat aagttcggca ttcccgcgtt tacgcccaca ttcctgaacg tgtcgtttat
     5161 cgtattcgcg ctgtttttcg tgccgtattt cgatccgccc gttaccgctc tggcatgggc
     5221 ggtttttgtc ggcggtattt tgcaactcgg cttccaactg ccctggctgg cgaaactggg
     5281 ctttttgaaa ctgcccaaac tgaatttcaa agatgcggcg gtcaaccgcg tgatgaaaca
     5341 gatggcgccg gcgattttgg gcgtgagcgt ggcgcaggtt tctttggtga tcaacacgat
     5401 tttcgcgtct tatctgcaat cgggcagcgt ttcatggatg tattacgccg accgcatgat
     5461 ggaactgccc ggcggcgtgc tgggggcggc actcggtacg attttgctgc cgactttgtc
     5521 caaacactcg gcaaaccaag atacggaaca gttttccgcc ctgctcgact ggggtttgcg
     5581 cctgtgtatg ctgctgacgc tgccggcggc ggtcggactg gcggtgttgt cgttcccgct
     5641 ggtggcgacg ctgtttatgt accgcgaatt cacgctgttt gacgcgcaaa tgacgcaaca
     5701 cgcgctgatt gcctattctt tcggtttaat cggtttaatc atgattaaag tgttggcgcc
     5761 cggcttttat gcgcggcaaa acatcaaaac gcccgtcaaa atcgccatct tcacgctcat
     5821 ttgcacgcag ttgatgaacc ttgcctttat cggcccactg aaacacgtcg gactttcgct
     5881 tgccatcggt ctgggcgcgt gtatcaatgc cggattgttg ttttacctgt tgcgcaaaca
     5941 tggtatttac cggcccggca agggttgggc ggcgttcttg gcaaaaatgc tgctcgcgct
     6001 cgccgtgatg ggaggcggcc tgtatgccgc ccaaatctgg ctgccgttcg actggacaca
     6061 caccggcgga atgcaaaagg ccgcccggct cttcatcctg attgtcatcg gaggctgcct
     6121 gtatttcgca tcactggctg cactgggctt ccgtccgcgc catttcaaac gggtggaaag
     6181 ctgaccgatg ccgatatcct ttttcggcaa acgctttttc cacgcctata ttcgggcgcg
     6241 catcccccgc gtccgcaccg gccggacggg ctgccgcatc atcaatgccg tctgaaaaca
     6301 agaaaaccga taccttatga ttttgacacc gccggacacg ccccttttcc tccgcaacgg
     6361 caatgccgac acgattgccg ccaaattcct gcaacgcccc gcacctgcat accgccgcga
     6421 gctgcttccc gacagcacgg gtaaaaccaa agtcgcctac gacttttcag acggcatttc
     6481 gcccgatgcg ccgctggtcg tgctgtttca cggtttggaa ggaagcagcc gcagccatta
     6541 cgcggtcgaa ctgatgcttg cggtacgcga tcggggttgg cacggcgtag tcgtccattt
     6601 ccgcagctgc ggcggcattg ccaacaccgc tccggtgttc taccacttgg gcgataccgc
     6661 cgaaatcgcc tttactttgg acacgttcgc cgcgcgttac cgtgaaatat acgccgtcgg
     6721 cgtatcgctg ggcggcaacg cgctggcaaa atatttgggc gaacagggca aaaaggcatt
     6781 gccgcaagcc gctgccgtca tctccgcccc cgtcgatgca gaggcggcag gcagacgctt
     6841 cgacagcggc atcacgcggc tgctctacac gcgctacttc ctccgcaccc tgatacccaa
     6901 agcaaaatcg ctccaaggtt ttcagacggc atttgccgca gggtgcaaaa cactgggcga
     6961 gtttgacgac cgcttcaccg caccgctgca cggctttgcc gaccggcacg actactaccg
     7021 ccaaacttcc tgcaaaccgc tgctcaaaca cgttgccaaa ccgctgctcc tgctcaatgc
     7081 cgtcaacgac cccttcctgc cgcccgaagc cctgccgata ccggacgaag tgtccgaagc
     7141 cgttaccctg ttccaacctg cacacggtgg tcatgtcggc tttgtcggca gcaccggcgg
     7201 caggctgcac ctgcaatggt tgccgcagac cgtcctgtcc tatttcgaca gcttccgcac
     7261 aaacagtcgt taacggtttg atgctaatat tccccctttc ccagacgaat acggaacccg
     7321 acatgaccga catcctcaat aaaatccttg ccaccaaagc acaggaagtt gccgctcaaa
     7381 aagccgccgt caacgccgaa cacatccgcg cacttgccgc agaagccgcg cccgtccgca
     7441 gcttcatcga ttcgatacgc ggcaaacacc gcctaaacct gcccgccgtc atagccgaaa
     7501 tcaaaaaggc aagcccgagc aaagggttaa tccgtccgga cttccgccct gccgagattg
     7561 cacgcgccta tgaaaacgcc ggagcggcgt gtttgtccgt actgaccgac gaaccctatt
     7621 tccaaggttc gcccgaatac ctcaaacagg cgcgcgaagc cgtatcgctg cccgtgctgc
     7681 gcaaagactt catcatcgac gaataccagg tttatcaggc gcgcgcatgg ggggcggata
     7741 ccgtcctgct gattgccgca gcactggaac aggaacaatt ggaacgcttt gaagcggtgg
     7801 cgcacgaatt gggcatgacc gtcctgctcg agctgcacga tgaaaccgaa ttggaaaaat
     7861 gccgcaacct gaccacgccg ttgtggggcg taaacaaccg caacctgagg acttttgaag
     7921 tctccctcga ccaaaccctg tcgctgctgc ccgcgctgga aggcaaaacc gtcgtaaccg
     7981 aaagcggcat tacaggcaag gcggatgtgg aatttatgca atcgcgcggt gtgcacacct
     8041 tcctgatcgg cgaaacgttc atgcgtgccg gcgatattga ggcggaagtg ggcaaactct
     8101 tctaaatccc gatttcagac ggcatattgc cgccaaccga ccaacaccct taccgagcac
     8161 ggaaccatca tgcaccgacc gaccgccaaa cagattctgc acgaagtatt tggttatccc
     8221 gaatttcgcg gcaggcagga ggctgtcatc aatactttgg caggcggcgg gagtttgacg
     8281 gtgctgatgc cgacgggcgg cggcaagtct ttgtgttacc agattcccgc gctgatgcgc
     8341 gaaggcgtgg cggttgtcgt atcgccgctg attgcgttga tgaacgacca agtggcaaac
     8401 ctgcacgccg ccggcatcga agcggcggca gtcaacagca gcacatcggc agatggggcg
     8461 cgcgagattg ccgacaagct tgcccaaggc cgtctgaaac tgctttatgt cgcgccggaa
     8521 cgcttggtta ccgaccgctt tttgcgtttt ctcgaccaac aaaccgtcag cctgttcgcc
     8581 attgatgagg cgcattgcgt cagccaatgg ggacacgatt tccgccccga gtaccaacag
     8641 ctcggcatgc ttgccgaacg ctatccgaac gtcccgcgca tcgccctgac cgccaccgcc
     8701 gatgccgcca cgcgcgccga catcaagcat tatctgcact tggacgatgc gcccgaattt
     8761 atctccagct ttgaccgccc gaatatttat tatcaggtta tcgaaaaaaa taatggcaaa
     8821 aaacaactgc ttgaattcat ccgcaaagaa atgacggggc aaagcggcat tgtgtattgc
     8881 ctaagccgca aaaaagtcga agatgtggcg cagtttttgc gtgaaaacgg attaaacgcg
     8941 attccgtatc atgccggttt gagcatggac gtgcgcgagg aaaaccaacg ccgctttacg
     9001 cgtgaagaca atattatcgt ggtggcgacc gtggcgttcg gtatgggcat agacaaaccc
     9061 gacgtgcgct ttgtcgccca tctcgatatg ccccagagtg tcgaacattt ctatcaggaa
     9121 tcggggcgcg ccggacggga cgggctgcct gccgtgagtt ggctgtgtta cggtttgaac
     9181 gattgggtgt tgctgcacga acggattgcc gaaggcaaca gcgacgaggt gcaaaagcaa
     9241 atcgaaatgc aaaaactcga tgccatgctt gccgtctgcg aaaccgccgc ctgccgccgc
     9301 gtactgctgc tcaaacattt cggcgaagca tccgaaccct gcggccattg cgacaactgc
     9361 ctgcatccgc ccgtacggtt tgacggcacg gtgttggtgc aaaaattact cagctgcgtg
     9421 taccgcgccg gacaacgttt tgccgccggt tacatcacca accttttgcg aggtaaaagc
     9481 gacgattgga tacgcggcaa ccggcacgaa caactgtcca cattcggcat cggtacggag
     9541 ttgtccaaca aagaatggcg cagcgtcatc cgccagtgta tcagcctcgg ctacctcacc
     9601 gtcaacatta cccgatatca ggcattgcaa ctgaccgaag ccgccaaaaa agtcctcaaa
     9661 ggcgaaaccg aagtgatgct gcgtccgctc aagcgcgaca agcccgccgc ccgcaccctc
     9721 aaagacaact ggctgcgtac cgaacgcgaa gaacgcctgt ggcaggcatt gcgcgtttgg
     9781 cgtatgaaac aggcagaagc cgaaggcatc cccgcctata tgattttcgg cgacaaaacc
     9841 ctgcgcgacc ttgtcgaaaa aatgccgcaa gacctcaacg gtctgcacga catctacggc
     9901 ttgggcgaag ccaaaatcga ccgtttcgga cacggtatcc tcgaagtctg ccgcaacgcc
     9961 gccgacttta gccacgatgc cgtcatccgt ccgcaaaccg aacgcgaaca acaactgcgt
    10021 caaaaactcg aagcctggcg gtatgaacag gcaagggcgg aaaactgcgc cctgcatgcc
    10081 gtcctctccg acgaaagcct tgccgatatg cttgccgcca cgcccgaaac cgaaaccgac
    10141 ctcgaaggcg tgcacggctt gggcagcgta cgcgccgcca aatacggacg ggacatcctc
    10201 gccgtctgcc gtccgttttc agacggcatc gatgaagccg ccaaacacaa acgccgcctg
    10261 atgcgcgccc tgattcaatg gtgcggcgaa acggcaaaac acgaacagtc cgaaccctac
    10321 cgcattctca gcaaagccgc cctgcgcgcc attgccgcca aacagccgga aggtttggcg
    10381 gagcttgccg ccgtatatgg cgtaggcgaa gaaaaagccg cacgttacgg tgcggcggtg
    10441 ttggcggtat tggaacgaaa tgccgtctga agcccgttac caagtttcag acggcattgc
    10501 ctctatttaa aaattcct
//
LOCUS       DAWHSK010000041        10496 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.041, whole genome shotgun sequence.
ACCESSION   DAWHSK010000041 DAWHSK010000000
VERSION     DAWHSK010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10496
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.041"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..2054)
                     /locus_tag="WIC03_000714"
     CDS             complement(<1..2054)
                     /locus_tag="WIC03_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEZ3883248.1"
                     /translation="MRLTHIKLSGFKSFTDPTTIHVPGQLVAVIGPNGCGKSNVIDAV
                     RWVLGEASAKQLRGESMQDVIFNGAATRRPAPRASVELVFDNSDHSLQGAWGQYAEVS
                     IKRQLTRQGESTYFINNQTVRRRDITDLFLGTGVGARGYAVIEQGMISRIIEARPEEL
                     RAYIEEAAGVSKYKERRKETEGRLKDTREHLQRLGDLQNELARQVEKLEKQAETAERY
                     KSLTAQLNRQQDLLDYAQWQQSLAAADKATEQHQSLQAQQDETAAQVQALNDEVHALQ
                     TAEQSQQQAVHELSNKRGVLREQIARLEEQIRHQQNLHQRIERDKQAAQAQMQRIHQE
                     QQQIRVQLEENELYVEEKQTELAEWAMQVAEHEERLPELEETQTALNAAFQTQQDEAN
                     RIRRELALKQQQLAHAEQTVAKHEERKGRLKQENQALNLPDEAETAAAQEAAALLQSR
                     QEHYEEQIIAAEEALHAAREAFQTASNRFQSLKQQHITLQAQQQALSQILSQQQEAAD
                     FWQATGHAAAPQLWQHITAPAEWQHALSVILAERLHARDVPQGFVPPEPLPQGQAAWL
                     SDGLSGGIKKSLPVQALLNQIQAQPPFQTALHHWLDGVLCAPDLSYALAHQSDLGAHQ
                     IWLTPEGHQVDKVSVLLYAKPAQESLIAQKARLDGIASELESLAPELSAAEAAFKQAE
                     AAV"
     gene            2056..2307
                     /locus_tag="WIC03_000715"
     CDS             2056..2307
                     /locus_tag="WIC03_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009345706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883249.1"
                     /translation="MVGKNKKEAYFNGNPAAPPHILPAKPYRIPHPRCRLKPSDGIFN
                     APEPRFRQCPMLGAAFAVLPRRRARYNPPRHPPDLETCL"
     gene            2304..3026
                     /gene="lpxH"
                     /locus_tag="WIC03_000716"
     CDS             2304..3026
                     /gene="lpxH"
                     /locus_tag="WIC03_000716"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEZ3883250.1"
                     /translation="MKPAYFISDLHLSEKHPELTALLLRFLRSSAAGQARAVYILGDL
                     FDFWVGDDEVSELNTSIVREIRKLSDKGVAVFFVRGNRDFLIGQDFCRQAGMTLLPDY
                     SVLDLFGCKTLICHGDTLCTDDRAYQRFRKIVHRKRLQKLFLMLPLKWRTRLAAKIRR
                     VSKMEKQVKPADIMDVNAAFTARQVRAFNAERLIHGHTHREHIHHENGFTRIVLGDWH
                     NDYASILRVDGDGAVFVPLEKY"
     gene            3295..4881
                     /locus_tag="WIC03_000717"
     CDS             3295..4881
                     /locus_tag="WIC03_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEZ3883251.1"
                     /translation="MALFLSIFPIVLLIWLMVKKNSMPSYVALPITAVLIYAIKLFYF
                     GDAGMLLNATAASGLVKTLTPITVIFGAIMFNRMMETTGCIDVIRKWLATISPNPVAQ
                     LMIIGWAFAFMIEGASGFGTPAAIAAPILMSLGFNPLKVAIFTLVMNSVPVSFGAVGT
                     PTWFGFAPLNLSAEDILAIGRQTGVMHFFAGFVIPVIGLGFIVPWSEIRKNLGFVAIA
                     VFSCTIPYVALAMVNEEFPSLVAGAIGLMVSVFAANQGWGLSKDHAKDPNAEKVPFAQ
                     VAKALAPLGMLIGMLVVTRIKQLGIKGILTSKEEWFSFQLPFDLSKITVSDSLTITFG
                     NIFGQDVSASYQTLYVPAWIPFVLTVWICILLYKTKFKDAWTIYSVTFNQTKKPLLAL
                     MGALIMVQLMLVGGDNSMVKIIGKEFAAMAGEHWVYFSPYLGAIGAFFSGSNTVSNLT
                     FGPIQQQIALDTGLSVTLILALQSVGGAMGNMVCLNNIIAVCTVLDVKNSEGAIIKKT
                     VIPMAIYGVIAVVAAMIFFL"
     gene            complement(5049..5252)
                     /locus_tag="WIC03_000718"
     CDS             complement(5049..5252)
                     /locus_tag="WIC03_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HEZ3883252.1"
                     /translation="MDNLNLQEISVLPENLPLYCSGPDNEQWNGHPRVFLPLCEGESG
                     SVACPYCGTRYRLDGKMPHHHYA"
     gene            5368..5865
                     /locus_tag="WIC03_000719"
     CDS             5368..5865
                     /locus_tag="WIC03_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-mimic protein DMP19"
                     /protein_id="HEZ3883253.1"
                     /translation="MTALTLPEDIRQQEPSALLYPLVSAYLEHTAQTGDESLSCLSDD
                     QHTLTAFCYLDSQVEEGGFVQLIASGYGEYIFRNPLADSLRRWKIKAVPKVLDKAKAL
                     YEQHGKTIETLADGGADIESLRKQFPDFEEWDGAYYEAAEQDLPLLAEHIQSNWETFA
                     HIGQA"
     gene            5947..7140
                     /locus_tag="WIC03_000720"
     CDS             5947..7140
                     /locus_tag="WIC03_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HEZ3883254.1"
                     /translation="MFFKHIEAAPADPILGLGEAFKAETRPEKVNLGIGVYKDASGAT
                     PIVKAVKEAEKRLLESETTKNYLTIDGVADYNEQTQILLFGKDHEIIASRRAKTAQSL
                     GGTGALRIATEFAKRQLNAQTIWISNPTWPNHNAIAKAVGIQDKPYRYYDAAKHGLDW
                     DGMIEDLSQAQKGDIVLLHGCCHNPTGIDPTPEQWETLAKLSAEKGWLPLFDFAYQGF
                     GNGLEEDAYGLRVFLKHNTELLIASSYSKNFGMYNERVGAFTLVAEDEETAARAHSQV
                     KTIIRTLYSNPASHGANTIALVLKNDDLKAQWIAELDEMRGRIKAMRQKFVELLKAKG
                     ASQDFDFIIEQNGMFSFSGLTPEQVDRLKNEFAIYAVRSGRINVAGITDDNIDYLCES
                     IVKVL"
     gene            complement(7543..8478)
                     /gene="hemC"
                     /locus_tag="WIC03_000721"
     CDS             complement(7543..8478)
                     /gene="hemC"
                     /locus_tag="WIC03_000721"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEZ3883255.1"
                     /translation="MNPKKLVIASRESLLAMWQAKHIQGRLKALYPDCEVEILGMTTR
                     GDRILDRTLSKVGGKGLFVKELEQALQDGRADLAVHSIKDVPMDLPEGFALAAIGERA
                     NPFDAFVSNRYARLEEMSKGAVVGTSSLRREAQLRASYPHLVIKPLRGNVQTRLSKLD
                     NGEYDAIILAAAGLQRLELDGRIRMILSESDSLPAAGQGALGIEIAAHREDLYEVLKP
                     LNHGVTNACVTAERALARALGGSCQVPLAAYCTEENGLLTLRGLVGHPDGSVVLRADA
                     QAPAEYADALGRAVAKKLADDGARELIGAVLNTEN"
     gene            8597..9112
                     /gene="ybeY"
                     /locus_tag="WIC03_000722"
     CDS             8597..9112
                     /gene="ybeY"
                     /locus_tag="WIC03_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEZ3883256.1"
                     /translation="MKRTKKYPFLTLQRQRFHLNFENASSAAGIPAERDFYRWAWSAL
                     KNEYRRADISLILLDEEEARAYNRDYRGKDYATNVLSFALNEGEILPCQVSEKLYGDL
                     IICPQVVLKEAAEQGKTPEQHFAHLTIHGTLHLMGYDHIEDDEAEIMEAEEIRLMLAA
                     GFPNPYQEDGH"
     gene            9114..9938
                     /locus_tag="WIC03_000723"
     CDS             9114..9938
                     /locus_tag="WIC03_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEZ3883257.1"
                     /translation="MDGAQPKTNFFERLIARLAREPDSAEDVLTLLRQAHEQEVFDAD
                     TLLRLEKVLDFSDLEVRDAMITRSRMNVLKENDSIERITAYVIDTAHSRFPVIGEDKD
                     EVLGILHAKDLLKYMFNPEQFHLKSILRPAVFVPEGKSLTALLKEFREQRNHMAIVID
                     EYGGTSGLVTFEDIIEQIVGDIEDEFDEDDSADNIHAVSSERWRIHAATEIEDINTFF
                     GTEYSSEEADTIGGLVIQELGHLPVRGEKVIIGSLQFTVARADNRRLHTLMATRVK"
     gene            complement(10068..>10496)
                     /locus_tag="WIC03_000724"
     CDS             complement(10068..>10496)
                     /locus_tag="WIC03_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEZ3883258.1"
                     /translation="QTIVILGMSLGGLLFALGAIPSLLDAVRSFLTNAGRATFSVAMT
                     SVGVNFLIGEQYLSILLSGETFKPVYDKLGLHSRNLSRTLEDAGTVINPLVPWSVCGV
                     FISHALGVPVWEYLPYAFFCYLSLALTLLFGWTGLTLSKK"
BASE COUNT         2458 a         3058 c         2640 g         2340 t
ORIGIN      
        1 acggcggctt ccgcctgttt gaacgcggct tcggcggcag aaagttcggg ggcgaggctt
       61 tccagttcgg aagcgatgcc gtcgaggcgc gctttttggg caatcaagct ttcctgcgcg
      121 ggcttggcat agagcagaac gctgacttta tcgacctgat ggccttcggg tgtgagccag
      181 atttggtgtg cgcccaaatc gctttgatgc gcgagggcgt agctcaaatc gggcgcgcac
      241 aatacgccgt cgagccagtg gtgcaatgcc gtctgaaacg gcggctgcgc ttggatttgg
      301 ttcagcaatg cctgtacggg cagggatttt ttgatgccgc cggagagacc gtctgaaagc
      361 cacgccgcct gcccctgcgg caaaggttcg ggcggtacga aaccttgcgg cacgtcgcgg
      421 gcgtgcaggc gttcggcaag gatgacggac agggcgtgct gccactcagc aggcgcggta
      481 atgtgttgcc acagttgcgg cgcggcggcg tggccggttg cctgccagaa atcggctgct
      541 tcctgctgtt gcgacaggat ttgcgacaac gcctgctgct gcgcctgcaa ggtgatatgt
      601 tgctgcttca ggctttggaa gcggtttgag gccgtctgaa acgcctcgcg ggcggcgtgt
      661 aaggcttctt cggcggcgat gatttgttct tcgtaatgct cttgccggct ttgcaacaag
      721 gcagcggcct cctgcgcggc ggcggtttcc gcttcgtcgg gcaggttcag ggcttggttc
      781 tcctgtttca gacggccttt gcgctcttcg tgcttggcaa cggtttgttc ggcatgggca
      841 agctgctgct gcttcaacgc cagttcgcgg cggatgcggt ttgcctcgtc ctgctgggtt
      901 tggaaggcgg cattgagcgc ggtttgggtt tcttccaatt cgggcagacg ctcttcgtgt
      961 tcggcaacct gcatcgccca ctccgccagc tcggtttgct tttcttcgac gtataactcg
     1021 ttttcttcaa gctgcacgcg gatttgctgc tgctcttgat gaatacgctg catctgcgcc
     1081 tgcgccgcct gcttgtcgcg ttcgatgcgt tggtgcaggt tttgctgatg gcggatttgt
     1141 tcttccaaac gggcaatctg ctcgcgcaac acgccgcgtt tgttgctcaa ttcatgcacc
     1201 gcctgttgct gcgactgttc ggcggtctgc aaggcgtgta cttcgtcgtt taacgcctga
     1261 acctgcgcgg cggtttcgtc ctgctgcgcc tgcaaagact gatgctgctc ggtcgccttg
     1321 tcggcggcgg caagcgattg ctgccattgg gcgtaatcga gcaaatcctg ttgccggttc
     1381 aactgcgcgg tcagggattt gtagcgttcg gcggtttccg cctgtttctc cagcttttcc
     1441 acctgacgcg ccaattcgtt ctgcaaatcg cccaaacgct gcaaatgctc gcgcgtgtct
     1501 ttcagacggc cttccgtctc cttgcggcgt tccttatatt tggacacacc tgccgcctcc
     1561 tcgatatagg cgcgcaattc ctccggccgc gcttcgatga tgcgcgaaat catcccctgc
     1621 tcgataacgg cataaccgcg cgcgcccacg cccgtaccca aaaataaatc ggtaatgtcg
     1681 cggcggcgca cggtctgatt attgatgaaa taagtcgatt cgccctgacg cgtcagctgc
     1741 cgcttgatac tcacctcggc atactgcccc cacgccccct gcaaactgtg gtcgctgttg
     1801 tcaaacacca gctccaccga agccctcggc gcaggacggc gcgtcgccgc accgttaaaa
     1861 atcacgtcct gcatactctc gccacgaagc tgcttcgccg aagcctcgcc caacacccag
     1921 cgcaccgcgt caatcacatt cgacttgccg cagccgttcg gcccgataac cgcaacaagc
     1981 tgccccggaa catgaatcgt ggtcgggtcg gtaaaagatt tgaagccgga gagtttgatg
     2041 tgggtcaggc gcataatggt cggaaaaaat aaaaaagaag cgtattttaa cggaaatccc
     2101 gccgcaccac cccatatctt gccggcaaaa ccttaccgca tcccgcaccc gcgatgccgt
     2161 ctgaagcctt cagacggcat ttttaacgcg cccgaacccc ggttccgcca atgcccgatg
     2221 cttggcgcgg catttgccgt attgccccgc cgccgggcgc gttataatcc gccacgacat
     2281 cctcccgatt tggaaacctg cctatgaaac ccgcctattt catctccgac ctgcatttga
     2341 gcgaaaagca ccccgaactg accgcgctgt tgctgcgttt tttacgttct tccgccgccg
     2401 ggcaggcgcg ggcggtttat attttgggcg atttgtttga tttttgggtg ggcgatgacg
     2461 aagtttccga gttgaatact tcgattgtgc gggaaatcag gaaattgtcc gacaaaggcg
     2521 ttgccgtgtt cttcgtcagg ggcaaccgcg atttcctaat cggtcaggat ttttgccgac
     2581 aggcgggtat gacgctgctg ccggattact cggttttgga cttgttcggc tgcaaaaccc
     2641 tcatctgcca cggcgacact ctgtgtacag atgacagggc ataccaacgt ttccgcaaaa
     2701 tcgtgcatcg gaagcggctg caaaaactgt tcctgatgct gcccctgaag tggcgcacgc
     2761 gccttgccgc caaaatcagg cgtgtcagca aaatggaaaa acaggtcaag cccgccgata
     2821 ttatggatgt caatgccgcc tttaccgcgc ggcaggttcg cgcctttaac gcggaaaggc
     2881 tgatacacgg gcatacccac cgcgagcata tccatcacga aaacggcttt acccgcatcg
     2941 ttttgggcga ctggcataac gactatgctt caatcctccg cgtagacggt gacggcgcgg
     3001 tattcgtgcc gctggaaaaa tactgaaaat gccgtctcaa gcctttcggg cggctttttt
     3061 tacacgcccc tttcccacac tccctccgcc gcatcatcac catccttaac ccgcattaat
     3121 gggcgttatc gataaaatgc ttgaccgatt acataagatt acgtaaagtg tgcaaacgca
     3181 taacaattgg tcttaccaat ttatctttta accaaaatat cgggtgtacg ggatttgaac
     3241 gtctttccaa acctcgacgc gccttatgat gcaaatgcct aaaaaggatt cgttatggca
     3301 cttttcctca gcatattccc catcgtcctg ctgatttggc tgatggtgaa aaaaaacagt
     3361 atgccctcct acgtcgcgct gccgattacc gcagtgctga tttacgccat caaacttttc
     3421 tacttcggcg atgcgggcat gctgctcaac gccaccgccg cttccggcct cgtcaaaacg
     3481 ctcacgccga ttaccgtgat tttcggcgcg attatgttca accgtatgat ggaaaccacg
     3541 ggctgcattg atgtcatccg caaatggctg gcgaccatca gccccaaccc cgtagcgcaa
     3601 ctgatgatta tcggctgggc ttttgccttt atgattgaag gcgcatccgg cttcggcacg
     3661 cctgccgcga ttgccgcgcc gattctgatg agcttgggct tcaacccgtt gaaagtggcg
     3721 attttcactt tggtgatgaa ctccgtcccc gtctctttcg gcgcggtcgg tacgccgacc
     3781 tggttcggtt tcgcaccgct gaacctgagt gccgaagaca tcctcgccat cggcaggcag
     3841 accggcgtaa tgcacttctt cgcaggtttc gtcatccccg tcatcggctt gggcttcatc
     3901 gtaccttggt ctgaaatccg caaaaacttg ggcttcgtcg ccattgccgt cttctcctgc
     3961 accattcctt atgtcgcatt ggcgatggtc aacgaagaat tcccgtcgct cgtcgccggc
     4021 gcaatcggcc tgatggtgtc cgtattcgcc gccaaccaag gctggggctt gagcaaagac
     4081 cacgccaaag acccgaatgc cgaaaaagtg ccgttcgccc aagtcgccaa agcactcgcc
     4141 cctttgggta tgctgatcgg catgctggtg gttacgcgca tcaaacagct cggcatcaaa
     4201 ggcattttga ccagcaaaga agaatggttc agcttccaac tgccgtttga tttgtccaaa
     4261 atcaccgtca gcgactccct gacgattacc ttcggcaata ttttcggaca agatgtcagc
     4321 gcgtcttacc aaacgctgta cgtcccggct tggattccgt ttgtgctgac cgtttggatt
     4381 tgcatcctgc tgtataaaac caaattcaaa gatgcctgga cgatttattc cgtaactttc
     4441 aatcaaacca aaaaaccgct gcttgccctg atgggcgcgc tgattatggt tcagctgatg
     4501 ctggtcggcg gcgacaattc gatggtgaaa atcatcggta aggaatttgc cgcaatggcg
     4561 ggcgaacact gggtttattt ctcgccgtat ctgggcgcga tcggtgcgtt cttctccggc
     4621 tccaacaccg tgtccaacct gactttcggc ccgattcagc agcaaatcgc cttggatacc
     4681 ggcctgtccg ttaccctgat tctggcgttg cagtccgtcg gcggcgcgat gggcaatatg
     4741 gtgtgcctca acaacatcat cgccgtatgt accgtattgg atgtgaaaaa ttccgaaggt
     4801 gcgattatca agaaaaccgt tatcccgatg gcgatttacg gcgtgattgc cgtcgtcgcg
     4861 gcaatgattt tcttcctcta ggcgaaacgc caccatccat agcggcagca cgcggggaac
     4921 ggaaaccgcc gttccccgtc tgggcggcgc gtcgggcaga atcccggtgc cggaacagcc
     4981 cgccccgcca aacaaatgcc gtctgaaacc ggaaaaggct tcagacggca tttttccgcc
     5041 gtttgcattc aggcgtaatg atgatgcggc atcttgccgt caaggcggta gcgcgtgccg
     5101 caatacgggc aggcaacgct gcccgattct ccttcgcaca aaggcaaaaa cactctcgga
     5161 tgcccgttcc actgctcgtt gtcgggtccc gagcaataca gcggcagatt ttccggcaac
     5221 acggaaattt cctgcagatt cagattgtcc atttgatttt cctttgcgtg gttggcgtgc
     5281 gcccatttta cgccatcggc aggctaaagg atattttcgg cgcaaagccg caatccgcta
     5341 taatccccac ttttcagacg gcataccatg actgcgctta cccttccgga agacatccgc
     5401 caacaagagc catccgccct gctctatccc ctcgtttccg cctacctcga acacaccgcc
     5461 caaaccggcg acgaatccct ctcctgcctg tccgacgacc agcatacgct gaccgcattc
     5521 tgctacctcg acagccaagt cgaagaaggc ggcttcgtgc aactcatcgc ctccggttac
     5581 ggcgaatata ttttccgcaa cccgcttgcc gacagcctgc gccgctggaa aatcaaagcc
     5641 gtgccgaaag tcttggacaa agccaaagcc ctctacgaac aacacggcaa aaccatcgaa
     5701 acgctcgccg acggaggcgc agacatcgaa tccctgcgca aacagttccc cgactttgaa
     5761 gaatgggacg gcgcatacta cgaagccgcc gaacaagacc tgcccctgct tgcagaacac
     5821 atacagtcaa actgggaaac cttcgcccat atcgggcagg cgtgattgcg tctgtttcca
     5881 gcccgtgtaa aacagcgtaa aatcggcaaa cccgaatgac cttccgtccc catcaaggag
     5941 caagctatgt tcttcaagca catcgaagcc gcccccgccg atccgattct cggtttgggc
     6001 gaagcgttca aagccgaaac ccgccccgaa aaagtcaacc tcggcatcgg cgtttataaa
     6061 gacgcatccg gcgcgacacc tattgtcaaa gccgtcaaag aggctgaaaa acgcctgttg
     6121 gaaagcgaaa ccaccaaaaa ctacctcacc atcgacggtg ttgccgacta caacgagcaa
     6181 acccaaatcc tgctgttcgg caaagaccac gaaatcatcg ccagccgtcg cgccaaaaca
     6241 gcgcaaagcc ttggcggtac aggcgcattg cgtattgcga ccgagtttgc caaacgtcag
     6301 ttgaacgcgc aaaccatctg gatttccaat ccgacttggc caaaccacaa cgccatcgcc
     6361 aaagcggtcg gtatccaaga caaaccttat cgttactatg atgccgccaa acacggtttg
     6421 gattgggacg gtatgattga agacttgagc caagcgcaaa aaggcgacat cgtcctgctg
     6481 cacggctgct gccacaaccc taccggcatc gaccctacgc ccgaacaatg ggaaactctg
     6541 gcaaaacttt ctgccgaaaa aggctggctg ccgctgtttg actttgccta ccaaggcttc
     6601 ggcaacggtt tggaagaaga tgcctacggc ttgcgcgtgt tcttgaaaca caatacagaa
     6661 ttgctgattg ccagctctta ttccaaaaac ttcggtatgt acaacgagcg cgtcggcgcg
     6721 ttcactttgg tggccgaaga tgaagaaaca gcagcccgcg cccacagcca agtcaaaacc
     6781 atcatccgta ccttgtattc caacccggct tcacacggtg cgaacaccat tgcgctggtg
     6841 ttgaaaaatg atgatttgaa agcacaatgg attgccgaac tcgatgaaat gcgcggccgc
     6901 atcaaagcca tgcgccaaaa atttgtcgag ttgctcaaag ccaaaggtgc aagccaagac
     6961 tttgatttca ttatcgaaca aaacggtatg ttctctttca gcggtctgac tcccgaacaa
     7021 gttgaccgtt tgaaaaacga gtttgccatt tatgccgtcc gctccggccg catcaacgtt
     7081 gccggcatta ccgacgacaa catcgattat ctgtgcgaaa gcatcgtgaa agtactgtaa
     7141 aaagcacagc catatgaaaa tgccgtctga accttaacca gttcagacgg catctgtttt
     7201 ttgttaaaaa acaataaact ataacaattt caaacaagct aaaaacatct ataaaatcaa
     7261 cccaatatga tgaactcaaa taaaaatacg aatatcatca ttctcaaatt caaaatttga
     7321 acgccacagt attatccgaa gccacaaaaa cctttccgcc gtcattcccg cgcaggcggg
     7381 aatccgggaa accaatgctg caagaattga ttagaaacaa ctgaaaccga gcaaactgga
     7441 ttcccacctg cgcgggaatg acgaaataag gcgagtaaaa ctgatgaaac aaattgatta
     7501 aaaacaataa cttatattat tttatttaaa ttttcgataa aatcaatttt ccgtattcaa
     7561 tactgctcca atcaattccc gcgcaccgtc gtccgccaat ttcttagcga cagcgcgtcc
     7621 gagcgcgtcg gcatattcgg caggggcttg cgcgtccgcc cgcaacacaa ccgaaccgtc
     7681 gggatgaccg accaagccgc gcaaggtcag caagccgttt tcttccgtgc aatatgcggc
     7741 cagcggcact tggcagcttc cgcccaaagc gcgtgcgagg gcgcgctcgg cggtaacgca
     7801 ggcattggta acgccgtggt tcaagggttt caaaacttca tacaaatctt cgcggtgcgc
     7861 ggcaatttcg atacccaacg cgccttgtcc ggcggcaggc aggctgtcgg attccgacaa
     7921 aatcatacgg atgcgtccgt ccaattctaa acgctgcaaa ccggcggcgg caaggataat
     7981 cgcgtcgtat tcgccattgt cgagtttgga caaacgggtt tgcacattgc cgcgcagggg
     8041 tttgatcacc aaatgcggat agctcgcacg caactgggct tcgcggcgca ggctggatgt
     8101 accgacgacg gcacctttgg acatttcctc caaacgcgcg tatcggttgg acacaaacgc
     8161 gtcaaacgga ttggcgcgtt cgccgatggc ggcaagggcg aaaccttcgg gcaaatccat
     8221 cggcacatcc ttaatcgaat gcaccgccaa atcggcgcgc ccgtcttgaa gggcttgttc
     8281 caactctttg acaaacaagc ctttgccgcc gacttttgac aaagttctgt ccaaaatccg
     8341 gtcgccgcgc gtggtcatac ccaaaatctc gacttcacaa tcgggataca gggctttcag
     8401 acggccttgg atatgctttg cctgccacat ggcaagcagg ctttcgcggc tggcgatgac
     8461 gagttttttc gggttcatgt tcaatgcgtt caaagaatgg aaacggcgtg aagtctatca
     8521 tattttgaac gtttcttcag acggcaccgt caggtctatg ttatatttac acccacaaaa
     8581 cacgaaaccc gccacaatga aacgcaccaa aaaataccct ttcttaacgt tgcagcggca
     8641 acgtttccat ttgaactttg aaaacgcctc ttccgccgcc ggcatcccag ccgaacgcga
     8701 tttctaccgc tgggcgtggt ctgccttgaa aaatgaatac cgccgcgccg acatcagcct
     8761 gattcttctg gacgaagaag aagcccgagc ctacaaccgc gactaccgcg gcaaagatta
     8821 cgccaccaat gtattgagtt tcgcgctcaa cgaaggcgaa atcctgccct gccaagtttc
     8881 ggaaaaactg tacggcgatt tgattatctg cccgcaagtg gttttgaaag aagccgccga
     8941 acaaggcaaa acacccgaac agcattttgc ccacctgacc atacacggta ctttacacct
     9001 gatgggctac gaccatatcg aagacgatga agccgaaata atggaagccg aagaaatccg
     9061 cctgatgctg gcggcaggct tccccaaccc ctaccaagag gacggacatt aaaatggacg
     9121 gcgcacaacc gaaaacaaat ttttttgaac gcctgattgc ccgactcgcc cgcgaacccg
     9181 attccgccga agacgtattg accctgttgc gccaagcgca cgaacaggaa gtatttgatg
     9241 cggatacgct tttaagattg gaaaaagtcc tcgatttttc tgatttggaa gtgcgcgacg
     9301 cgatgattac gcgcagccgt atgaacgttt taaaagaaaa cgacagcatc gaacgcatca
     9361 ccgcctacgt tatcgatacc gcccattcgc gcttccccgt catcggtgaa gacaaagacg
     9421 aagttttggg tattttgcac gccaaagacc tgctcaaata tatgttcaac cccgagcagt
     9481 tccacctcaa atcgatattg cgccctgccg tcttcgtccc cgaaggcaaa tcgctgaccg
     9541 cccttttaaa agagttccgc gaacagcgca accatatggc gatcgtcatc gacgaatacg
     9601 gcggcacgtc gggtttggta acttttgaag acatcatcga gcaaatcgtc ggcgacatcg
     9661 aagacgagtt tgacgaagac gacagcgcgg acaacatcca cgccgtttct tccgaacgct
     9721 ggcgcatcca cgcggctacc gaaatcgaag acatcaacac cttcttcggt acggaataca
     9781 gcagcgaaga agccgacacc atcggcggcc tggtcattca agagttaggg catctgcccg
     9841 tacgcggcga aaaagtcatc atcggcagtc tgcaatttac cgtcgcacgc gccgacaacc
     9901 gccgcctgca tacgctgatg gcgacccgcg tgaagtaagc accgccgttt ctgcacagtt
     9961 taggatgacg gtacgggcgt tttctgtttc aatccgcccc atccgccaaa cataaaaatg
    10021 ccgtctgaag ccggaaaaca ggttcagacg gcattttgct tatccgctta ttttttgctc
    10081 aaagtcagcc ccgtccagcc gaataacagg gtcaaagcca aactcaaata gcagaaaaag
    10141 gcatagggca gatattccca gaccggcaca cccagcgcat ggctgataaa tacgccgcac
    10201 acgctccacg gaacaagcgg gttgatgacc gtccccgcat cttcgagcgt ccgagacagg
    10261 ttgcgcgaat gcagaccgag cttatcgtaa acgggtttga acgtttctcc cgaaagcagg
    10321 atgctcaaat attgctcgcc aatcaggaaa ttaaccccga ccgaagtcat agcaacgctg
    10381 aatgtggcac gcccggcatt cgtcaaaaag ctgcggacgg catccagcag ggaaggaatc
    10441 gcgcccagtg caaacaacag cccgcccaaa ctcatcccga ggataacgat agtctg
//
LOCUS       DAWHSK010000042        10457 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.042, whole genome shotgun sequence.
ACCESSION   DAWHSK010000042 DAWHSK010000000
VERSION     DAWHSK010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10457
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.042"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..866
                     /locus_tag="WIC03_000725"
     CDS             <1..866
                     /locus_tag="WIC03_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEZ3883259.1"
                     /translation="PGVIGLELGQALHRLGVKVEIFGLGGIIGGISDPVVSDEAKAVF
                     GEELKLHLDAKTEVKLDADGNVEVHWEQDGEKGVFVAEYMLAAVGRRPNVDNIGLENI
                     NIEKDARGVPVADPLTMQTSIPHIFIAGDASNQLPLLHEAADQGKIAGDNAGRYPNIG
                     GGLRRSTIGVVFTSPQIGFVGLKYAQVAAQYQADEFVIGEVSFKNQGRSRVMLVNKGH
                     MRLYAEKATGRFIGAEIVGPAAEHLAHLLAWAHQMKMTVPQMLDMPFYHPVIEEGLRT
                     ALRDADAKLKA"
     gene            1145..1522
                     /gene="sdhC"
                     /locus_tag="WIC03_000726"
     CDS             1145..1522
                     /gene="sdhC"
                     /locus_tag="WIC03_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEZ3883260.1"
                     /translation="MSAKPRPVYLDLPNIRLPIPGIVSILHRISGVGLFIMLPFLLYF
                     LSGTLSQESAFETYRAIVSHPLVKLVLIGVLWAYLHHSLAGIRFLFLDAHKGLELNTA
                     RNTAKAVFASALVLTVVLGALLW"
     gene            1516..1857
                     /gene="sdhD"
                     /locus_tag="WIC03_000727"
     CDS             1516..1857
                     /gene="sdhD"
                     /locus_tag="WIC03_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HEZ3883261.1"
                     /translation="MVERKLTGAHYGLRDWAMQRATAVIMLIYTVALLVVLFSLPKEY
                     SAWQAFFSQTWVKVFTQVSFIAVFLHAWVGIRDLWMDYIKPFGVRLFLQVATIVWLVG
                     CLVYSIKVIWG"
     gene            1860..3623
                     /gene="sdhA"
                     /locus_tag="WIC03_000728"
     CDS             1860..3623
                     /gene="sdhA"
                     /locus_tag="WIC03_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HEZ3883262.1"
                     /translation="MGFPVRKFDAVIVGGGGAGLRAALQLSKSGLNCAVLSKVFPTRS
                     HTVAAQGGISASLGNVQEDRWDWHMYDTVKGSDWLGDQDAIEFMCRAAPEAVIELEHM
                     GMPFDRVESGKIYQRPFGGHTAEHGKRAVERACAVADRTGHAMLHTLYQQNVRANTQF
                     FVEWTAQDLIRDENGDVVGVTAMEMETGEVYIFHAKAVMFATGGGGRIYASSTNAYMN
                     TGDGLGICARAGIPLEDMEFWQFHPTGVAGAGVLITEGVRGEGGILLNADGERFMERY
                     APTVKDLASRDVVSRAMAMEIYEGRGCGKNKDHVLLKIDHIGAEKIMEKLPGIREISI
                     QFAGIDPIKDPIPVVPTTHYMMGGIPTNYHGEVVVPQGDEYEVPVKGLYAAGECACAS
                     VHGANRLGTNSLLDLVVFGKAAGDSMIKFIKEQSDWKPLPANAGELTRQRIERLDSQT
                     DGENVDALRRELQRSVQLHAGVFRTDEILSKGVREVMAIAERVKRTEIKDKSKVWNTA
                     RIEALELDNLIEVAKATLVSAEARKESRGAHASDDHPERDDENWMKHTLYHSDTNTLS
                     YKPVHTKPLSVEYIKPAKRVY"
     gene            3744..4451
                     /locus_tag="WIC03_000729"
     CDS             3744..4451
                     /locus_tag="WIC03_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEZ3883263.1"
                     /translation="MEKMSFEIYRYNPDVDAKPYMQRYELELEPTDVKLLDALVRLKA
                     QDDTLSFRRSCREGICGSDGMNINGKNGLACLTDLRGLKQPVKIRPLPGLPVIRDLIV
                     DMTQFFKQYHSVKPYVVNDNPIDADKERLQTQEERKELDGLYECILCACCSTACPSFW
                     WNPDKFVGPSGLLNAYRFIADSRDTITNERLDNLNDPYRLFRCHTIMNCVDVCPKHLN
                     PTRAIGKIKEIMLKRAV"
     gene            4455..4703
                     /locus_tag="WIC03_000730"
     CDS             4455..4703
                     /locus_tag="WIC03_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HEZ3883264.1"
                     /translation="MMVFDDIAKRKIRFQTRRGLLELDLIFGRFMEKEFEHLSDKELS
                     EFSEILEFQDQELLALINGHSETDKGHLIPMLEKIRRA"
     gene            4815..6098
                     /gene="gltA"
                     /locus_tag="WIC03_000731"
     CDS             4815..6098
                     /gene="gltA"
                     /locus_tag="WIC03_000731"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HEZ3883265.1"
                     /translation="MSKSIKLNVPGQAGLELPVLEASIGHDVVDIRGLTKNTGLFSFD
                     PGFVSTASCESKITYIDGDQGLLYYRGYPIEQLAEKSDYLEVCYLLIYGELPTPEQKA
                     EFDNTVRRHTMVHEQLTWFFRGFRRDAHPMAMMVGVVGALSAFYQDSLDISNPEHRKI
                     AIYRLISKIPTIAAMCYRYSNGLPFNYPKNNLSYSENFLHMMFATPCEDYKPNPVLAR
                     ALDRIFILHADHEQNASTSTVRLAGSSGANPFACIAAGIACLWGASHGGANEAVLKML
                     DEIGDVSNVAAYMEGVKQRKYRLMGFGHRVYRNMDPRASIMRETCYEVLKELGLEDSP
                     KFKLAMELEQIALKDPFFIERKLYPNVDFYSGIVLSALGIPTEMFTVIFALSRSVGWI
                     SHWHEMISDPSLKIGRPRQLYTGSERRDYVPPGER"
     gene            6303..9131
                     /locus_tag="WIC03_000732"
     CDS             6303..9131
                     /locus_tag="WIC03_000732"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEZ3883266.1"
                     /translation="MMDEKLNFSYLFGSNAPYIEELYEAFLENPDAVDEKWKQYFTDL
                     SKQPGTVAVDVAHTPIRESFVTLAKKKIASAVAGGADEAMLKKQVSVLRLISAYRIQG
                     VGAAQLDPLKRIPPRDIEALDPKFHGLSDADMTLQFNMGEGDFANRGKLPLSQIISNL
                     KQTYCGHIALEYIYIPNTEERRWVRNYFESVLSTPHYNADQKRRILKEMTAAETLERY
                     LHTKYVGQKRFGVEGGESAIAGLNYLIQNASKDGVEEVIIGMAHRGRLNVLVNILGKK
                     PGDLFAEFEGRAEIKLPSGDVKYHMGFSSDIATPHGPMHVSLAFNPSHLEIVNPVVEG
                     SARAKQKRLGENGRDKVLPVLIHGDSAFIGLGVNQATFNLSKTRGYTTGGTVHIVINN
                     QIGFTTSDIRDTRSTVHCTDIAKMVSAPVIHVNGDDPERVCFAIQAALDYRKKFHKDI
                     VIDVVCYRKWGHNEGDDPTLTQPMMYKKVSQHPGARALYTEQLIAEGVVTQAEADGYI
                     QAYRDALDKGEHVEQTTLSNFQRTQIDWSKYQGKDWREHIETGLPAADIERLTEKFTA
                     VPEGFALHPTAKRVIEARKAMASGKQAIDWGMAETLAYASLVTKGHGVRISGEDSGRG
                     TFSHRHAVLHDQKREKWDDGTYVPLRHMGEGMGEFLVIDSILNEEAVMAFEYGFACSA
                     PDKLTIWEAQFGDFANGAQVTIDQFLSSGETKWGRLCGLTTILPHGYDGQGPEHSSAR
                     VERWLQLCSENNMQVIMPSEASQMFHLLQRQVLGSYRKPLVIFMSKRLLRFKGAMSPL
                     ENFTEGSTFRPVIGDTAERASNGSVKRVVLCAGQVYYDLEAGRTERKLEDDVAIVRVE
                     QLYPFPYEEVKAELAKYPNAKSVVWAQEEPKNQGAFYQIRHRIEDVISEEQKLSYAGR
                     PSSASPAVGYSSKHIAQLKQLVEDALAL"
     gene            9231..10412
                     /gene="odhB"
                     /locus_tag="WIC03_000733"
     CDS             9231..10412
                     /gene="odhB"
                     /locus_tag="WIC03_000733"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HEZ3883267.1"
                     /translation="MIIDVKVPMLSESVSEGTLLEWKKKVGEAVARDEILIDIETDKV
                     VLEVPSPQAGVLVEIVAQDGETVVADQVLARIDTAATAAAEAPVAAPAEAAPAAAPAA
                     AQNNAAMPAAAKLAAETGVDVNALQGSGRDGRVLKEDVQNAAAKPAAAAAPAVALPAG
                     ARPEERVPMSRLRARVAERLLASQQENAILTTFNEVNMKPIMDLRAKYKEKFEKEHGV
                     KLGFMSFFVKAAVAALKKYPVVNASVDGKDIVYHGYFDIGIAIGSPRGLVVPILRDAD
                     QMSIADIEQAIVDYAKKAKDGKIAIEDLTGGTFSITNGGTFGSMMSTPIINPPQSAIL
                     GMHATKERAVVENGQVVVRPMMYLALSYDHRIIDGREAVLTLVAIKDALEDPARLLLD
                     L"
BASE COUNT         2598 a         2587 c         2714 g         2558 t
ORIGIN      
        1 ggccgggtgt tatcggtttg gaactgggtc aggcattgca ccgtttgggc gtgaaagttg
       61 aaattttcgg tttgggcgga atcatcggcg gcatttccga ccccgtcgtt tcagacgagg
      121 cgaaagccgt gttcggcgaa gaattgaaac tgcatttgga tgctaaaacc gaggtcaagc
      181 tcgatgcaga cggcaatgta gaagtccatt gggagcagga cggcgaaaaa ggcgtatttg
      241 ttgccgaata tatgctggca gccgtgggcc gccgtccgaa cgttgacaat atcggtttgg
      301 aaaatatcaa tatcgaaaaa gatgcgcgcg gcgtacccgt tgccgacccg ctgaccatgc
      361 agaccagcat tccgcatatc tttattgcgg gcgacgcgtc caaccaactg cccctgctgc
      421 acgaagctgc cgaccaaggc aagattgccg gcgacaatgc aggccgttac ccgaatatcg
      481 gcggcggttt gcggcgcagc accatcggcg tggtgtttac cagtccgcaa atcggctttg
      541 tcggtctgaa atacgcgcag gttgccgcgc aataccaagc cgacgaattt gtcatcggcg
      601 aagtatcgtt caaaaaccaa ggccgcagcc gcgtgatgct ggtgaacaaa ggccatatgc
      661 gcctgtatgc cgaaaaagcc accggccgct ttatcggcgc ggaaatcgta ggccctgccg
      721 ccgaacattt ggcgcacttg ttggcatggg cgcatcaaat gaagatgacc gttccgcaaa
      781 tgctggatat gccgttctac catcccgtta tcgaggaagg tctgcgtacc gcgttgcgcg
      841 atgccgatgc gaaattgaaa gcctgaccga tatggcaaaa caatgccgtc tgaaattttt
      901 tcagacggca ttttgttttt ggggatgggg tcggatgctg ataccgtgtc gggaaggggg
      961 cggcaaaact aaaaatcttt cttttaatct gctgtttcca cgcgtgtttg tcaaaatcta
     1021 tcagtttgtt tttaaaatac actgttcaaa atgggataaa acaggtaaat taacgtttat
     1081 gtaacccagt gtagcaatgg gtttacggtt tttgagtcga tatataacta cagaggaatt
     1141 gactatgtct gccaaaccgc gtcctgttta tctggatttg ccgaacatcc gtctgccgat
     1201 acccgggata gtttccatcc ttcaccgcat cagcggggtc gggctgttta ttatgctgcc
     1261 tttcctgctg tatttcctgt ccggtaccct gagtcaagag tctgcatttg aaacttaccg
     1321 tgccattgtt tcccatcctt tggtcaagct ggttttaatc ggtgtattgt gggcttatct
     1381 gcaccattct ctcgccggta tccgcttttt atttttggat gcacacaaag gccttgagct
     1441 gaatactgcg cgcaataccg ctaaagccgt atttgcttct gcattggttt tgactgtcgt
     1501 tttgggagcg ttgttatggt agaacgtaaa ttgaccggtg cccattacgg tttgcgcgat
     1561 tgggcgatgc aacgtgcgac tgcggttatt atgttgattt ataccgttgc acttttagtg
     1621 gttctatttt ccctgcctaa agaatattcg gcatggcagg cattttttag tcaaacttgg
     1681 gtaaaagtat ttacccaagt gagcttcatc gccgtattct tgcacgcttg ggtgggtatc
     1741 cgcgatttgt ggatggacta tatcaaaccc ttcggcgtgc gtttgttttt gcaggttgcc
     1801 accatcgtct ggctggtcgg ctgcttggtg tattcaatta aagtaatttg ggggtaagta
     1861 tgggttttcc tgttcgcaag tttgatgccg tgattgtcgg cggtggtggt gcaggtttac
     1921 gcgcagccct ccaattatcc aaatccggtc tgaattgtgc cgttttgtct aaagtgttcc
     1981 cgacccgttc gcataccgta gcggcgcagg gcggtatttc cgcctctctg ggtaatgtgc
     2041 aggaagaccg ttgggactgg cacatgtacg ataccgtgaa aggttccgac tggttgggcg
     2101 accaagatgc gattgagttt atgtgccgcg ccgcgcctga agccgtaatt gagttggaac
     2161 acatgggtat gccttttgac cgtgtggaaa gcggtaaaat ttatcagcgt cctttcggcg
     2221 gccatactgc cgaacacggt aaacgcgcgg tagaacgcgc ctgtgcggtt gccgaccgta
     2281 caggtcatgc gatgctgcat actttgtacc aacaaaacgt ccgtgccaat acgcaattct
     2341 ttgtggaatg gacggcacaa gatttgattc gtgatgaaaa cggcgatgtc gtcggcgtaa
     2401 ccgccatgga aatggaaacc ggcgaagttt atattttcca cgctaaagct gtgatgtttg
     2461 ctaccggcgg cggcggccgt atttatgcgt cttctaccaa tgcctatatg aataccggcg
     2521 atggtttggg tatttgtgcg cgtgcaggta tcccgttgga agacatggaa ttctggcaat
     2581 tccacccgac cggcgtggca ggtgcgggcg tgttgattac cgaaggcgta cgcggcgagg
     2641 gcggtattct gttgaatgcc gacggcgaac gctttatgga acgctatgcg ccgaccgtaa
     2701 aagacttggc ttctcgcgac gttgtttccc gcgcgatggc gatggaaatc tacgaaggtc
     2761 gcggttgcgg taaaaacaaa gaccatgtct tactgaaaat cgaccatatc ggcgcagaaa
     2821 aaattatgga aaaactgccg ggcatccgcg agatttccat tcagttcgct ggtatcgatc
     2881 cgattaaaga cccgattccc gttgtgccga ctacccacta tatgatgggc ggcattccga
     2941 ccaattacca cggcgaagtc gttgtgccgc aaggagacga atacgaagtg cctgtaaaag
     3001 gcctgtatgc cgcaggtgag tgcgcctgtg cttccgtaca cggtgcgaac cgtttgggta
     3061 cgaactccct gctggacttg gtggtattcg gtaaagctgc cggcgacagc atgattaaat
     3121 tcatcaaaga gcaaagcgac tggaaacctt tgcctgctaa tgctggtgag ttgacccgcc
     3181 aacgtatcga gcgtttggac agccaaaccg atggtgaaaa cgttgatgca ttgcgccgcg
     3241 aactgcaacg ctccgtacaa ttgcacgccg gcgtgttccg tactgatgag attctgagca
     3301 aaggcgttcg agaagtcatg gcgattgccg agcgtgtgaa acgtaccgaa atcaaagaca
     3361 agagcaaagt gtggaatacc gcgcgtatcg aggctttgga attggataac ctaattgaag
     3421 tggcgaaagc gactttggtg tctgccgaag cacgtaaaga atcacgcggt gcgcacgctt
     3481 cagacgacca tcctgagcgc gatgatgaaa actggatgaa acatacgctg taccattcag
     3541 ataccaatac tttgtcttac aaaccggtac ataccaagcc tttgagcgtg gaatacatca
     3601 aaccggccaa gcgcgtttat tgatgcgttt tcagacggtt ttcgcctcat aagctcgtct
     3661 gaaatctaac catacccaca ttgaactgct tgaatttata atacaaaatc attgggcagt
     3721 tgatgagaaa aggaacactt ctcatggaaa aaatgagttt tgaaatttac cgttacaacc
     3781 cggatgttga tgccaagcct tatatgcagc gttacgagtt ggaattggaa ccgaccgacg
     3841 tgaaactttt ggatgctttg gtacgcctga aagcacaaga cgataccttg tctttccgcc
     3901 gctcctgccg cgaaggcatt tgcggatcgg acggtatgaa catcaacggc aaaaacggct
     3961 tggcgtgttt gaccgatctg cgtggcttga aacagccagt taaaatccgt cctctgccag
     4021 gtctgcctgt tatccgcgac ctgattgtgg atatgaccca gttcttcaaa caataccatt
     4081 ccgtcaaacc ttatgttgtc aacgataatc cgattgatgc ggacaaagag cgtctgcaaa
     4141 ctcaggaaga gcgtaaagag ttggacggtt tgtacgagtg tattttgtgc gcctgctgtt
     4201 cgactgcctg cccgtcattt tggtggaacc ctgataaatt cgttggtccg tccggtttgc
     4261 tgaatgctta ccgtttcatt gcggacagcc gtgataccat cactaatgag cgtttggata
     4321 atctgaacga cccataccgt ttgttccgtt gccacaccat tatgaactgc gtagacgtat
     4381 gtcctaaaca cttgaatccg acccgagcca tcggtaagat taaagagatt atgttgaaac
     4441 gggccgttta agaaatgatg gtttttgacg atattgccaa acggaaaatc cgttttcaaa
     4501 cccgccgggg attgttggaa ttagatttaa tcttcggcag gtttatggaa aaagaattcg
     4561 agcatttgag cgataaagag ctgtccgagt tttccgaaat ccttgaattt caagatcaag
     4621 aattgcttgc cttgattaac gggcattcgg aaacggacaa agggcacctt atcccgatgc
     4681 ttgaaaaaat cagacgggca tgaggcatct gaaatctgcc tgcaggcaga tttcaaaatg
     4741 caaaagccgt ctgaaggcaa agaacgtgct gcggatgcag taacgtgggt tataacttgc
     4801 aaaggagcaa taatatgtcc aaatcaatca aactcaacgt accgggtcag gcaggtttgg
     4861 agctgccggt attggaagcc agcatcgggc acgatgtggt tgacattcgg gggctgacaa
     4921 aaaatacagg tttgttttcc ttcgaccccg gatttgtttc aaccgcaagc tgtgagtcta
     4981 aaattactta catcgacggc gatcaaggct tgctttatta tcgcggatac cccatcgagc
     5041 agctggccga aaagtccgat tatttggaag tctgctacct gttgatttac ggcgaactgc
     5101 cgactcccga gcaaaaggca gaatttgaca ataccgtccg ccgccacacg atggtgcatg
     5161 aacagctgac ttggttcttc cgggggttcc gccgcgacgc gcatccgatg gcgatgatgg
     5221 tcggcgtggt cggcgcactg tctgcgttct accaagacag cttggacatt agcaatcccg
     5281 aacaccgcaa aatcgcgatt taccgcctga tttctaaaat cccgaccatt gcggcaatgt
     5341 gctaccgcta ttcaaacggt ctgccgttca attatccgaa gaataatctt tcttattccg
     5401 aaaacttcct tcatatgatg ttcgccacgc cgtgtgaaga ctacaaaccc aatcccgttt
     5461 tggcacgcgc gctcgaccgc atctttattt tgcatgccga ccacgagcaa aacgcctcaa
     5521 cttcaaccgt ccgtctggca gggtcttcgg gtgcgaaccc gtttgcctgt attgctgccg
     5581 gtatcgcctg cctgtggggt gcttcgcacg gcggtgcgaa cgaagccgtg ttgaaaatgt
     5641 tggacgaaat cggcgatgtg tctaatgttg ccgcatacat ggaaggtgtg aaacaacgca
     5701 aataccgtct gatgggcttc ggtcaccgcg tgtaccgcaa tatggatccg cgtgccagca
     5761 ttatgcgcga aacctgctat gaagttttga aggaattggg cttggaagac agtccgaaat
     5821 tcaaactggc gatggaattg gaacagattg cgctgaaaga cccgttcttt atcgaacgca
     5881 aactgtatcc aaacgtcgat ttctattccg gcatcgtcct gtccgcgctg ggcatcccga
     5941 ccgaaatgtt taccgtcatc ttcgccctgt cgcgcagcgt gggctggatt tcgcactggc
     6001 acgagatgat tagcgatcct tcgctgaaaa tcggccgccc gcgccagctt tatactggtt
     6061 cggaacgccg cgattatgtg ccgccaggcg agaggtaaca tacattaata tattgtcaaa
     6121 caggcaatat cagagaaccg gattgtttcc cgaatccgtc tgattgtagt cggatgaaat
     6181 caagacaagc aatccggttt aaaatagggt agaataaaat gtcttttcag gcggcatcag
     6241 tttagccgtc aggacgcgga cttctaccct ttgtttatat tttaaagaaa agagcgcacg
     6301 ccatgatgga cgaaaaactc aatttctctt acctgttcgg ttcaaacgca ccttacattg
     6361 aggaattgta cgaggctttt ttggaaaacc ccgatgcggt tgatgaaaaa tggaagcagt
     6421 atttcaccga tttgagcaaa cagccgggga cggttgctgt cgatgtcgca cacacaccga
     6481 ttcgcgaatc atttgttact ttggcgaaaa agaaaattgc atctgccgtt gcgggcggtg
     6541 cggatgaggc aatgctgaaa aagcaagtca gcgttttacg gctgatttct gcctatcgta
     6601 tccaaggcgt gggtgcagcc caacttgatc cgctcaaacg tatccccccg cgcgatattg
     6661 aagccctcga tccgaaattc cacggtctgt cagatgccga tatgacgctt caattcaata
     6721 tgggcgaggg cgattttgcc aatcgcggca aactgccttt gtcccaaatc atcagcaacc
     6781 tcaaacaaac ctactgcggc cacatcgcat tggaatatat ctatattccc aataccgaag
     6841 agcgccgctg ggtacgcaat tattttgaaa gcgtattgtc cacaccgcat tacaatgccg
     6901 atcaaaaacg ccgtatcttg aaagagatga ctgctgccga aactttggaa cgttatctgc
     6961 ataccaaata tgtcggtcag aaacgtttcg gtgtcgaagg cggcgaaagc gcgattgccg
     7021 gtttgaacta cctgattcaa aacgccagta aagacggcgt ggaagaagtc atcatcggta
     7081 tggcgcaccg tggccgtctg aatgttttgg tgaacatttt gggcaaaaaa cccggcgatt
     7141 tgtttgccga atttgaaggt cgtgccgaaa tcaaactgcc cagcggcgac gtgaaatacc
     7201 atatgggctt cagctccgat attgctaccc cgcacggccc gatgcatgtt tctttggcgt
     7261 tcaacccgtc tcacttagaa atcgtcaacc cggtggtgga aggttctgcg cgcgccaaac
     7321 aaaaacgttt gggcgaaaac ggccgcgaca aagtcttgcc ggtattgatt cacggcgact
     7381 ccgcatttat cggtctggga gtcaaccaag cgacattcaa cctgtctaaa acgcgcggtt
     7441 ataccaccgg cggtacggtt catatcgtca tcaacaacca aatcggcttt accacttccg
     7501 atatccgcga tacccgttca accgtacact gtaccgatat cgcaaaaatg gtttccgctc
     7561 cggttatcca tgtgaacggc gatgatcctg agcgtgtttg ctttgccatc caagccgctt
     7621 tggattaccg caaaaaattc cataaagaca tcgtgattga cgttgtctgc taccgtaaat
     7681 ggggtcacaa cgagggcgat gatccgacct tgacccaacc gatgatgtac aaaaaagtat
     7741 cgcaacaccc cggtgcgcgt gctttgtaca ccgagcaact gattgccgaa ggcgtggtaa
     7801 cccaagccga ggcagacggt tacatccaag cctaccgtga tgctttggac aaaggcgaac
     7861 acgtcgagca aacaacgttg agcaactttc aacgcacgca aatcgactgg agcaaatacc
     7921 aaggcaaaga ttggcgcgaa cacatcgaaa ccggtttgcc tgccgcagat attgagcgtc
     7981 tcactgagaa gtttaccgcc gtaccggaag gctttgccct gcatccgact gcaaaacgtg
     8041 tgattgaagc gcgtaaagcc atggcatccg gcaaacaagc catcgactgg ggtatggcgg
     8101 aaaccctcgc atacgccagc ctcgtaacca aaggccatgg cgtgcgtatt tccggcgagg
     8161 actctgggcg cggtacattc tcacaccgtc atgcggtatt gcacgatcaa aaacgcgaaa
     8221 aatgggacga cggtacttat gtccctctgc gccatatggg cgaaggtatg ggcgagttcc
     8281 tggttatcga ctccattttg aacgaagaag ccgtgatggc gttcgagtac ggctttgcct
     8341 gctccgcgcc tgacaagctg accatttggg aggctcaatt cggtgacttc gccaacggcg
     8401 cgcaagtgac tattgaccaa ttcctgtctt caggcgaaac caaatggggt cgtttgtgcg
     8461 gtctgaccac catcctgccg cacggctacg acggtcaagg ccccgagcac tcctctgcac
     8521 gcgtagaacg ttggttgcaa ctgtgttccg agaacaatat gcaagtcatc atgccgtctg
     8581 aagcgtcgca aatgttccac ctcttgcaac gccaagtttt gggttcatac cgcaaaccgc
     8641 tggtgatttt catgtccaaa cgcctgttgc gcttcaaagg tgcaatgagc ccgctggaaa
     8701 acttcaccga aggttcgact ttccgtccgg ttatcggtga taccgccgaa cgcgcaagca
     8761 acggcagcgt gaaacgtgtg gtattgtgtg ccggtcaggt ttactatgac ttggaagcag
     8821 gccgtaccga gcgtaaactg gaagatgatg ttgcgattgt ccgcgttgag cagctgtatc
     8881 cgttcccata cgaggaggtt aaagccgaac tggcgaaata tccgaacgca aaatctgtgg
     8941 tttgggcaca agaagagccg aaaaaccaag gtgcgttcta ccaaatccgc caccgcatcg
     9001 aagacgttat cagcgaagag caaaaactgt cttatgccgg tcgtccaagc agcgcatcgc
     9061 ctgcagtggg ctactcaagc aaacatattg cccaattgaa acaattggtt gaagacgctt
     9121 tggcgttgta agccaagtgg cattccgtct gaggctgctc aggcggaatg cccatatgca
     9181 gaatgaaaaa cacacaacag gccgtctgaa agggccattg gagacacaaa atgattattg
     9241 atgtaaaagt acctatgctg tctgaaagcg tatctgaagg cacgctcttg gaatggaaga
     9301 aaaaagttgg cgaagcagtt gcccgtgacg aaatcctgat cgatatcgaa acggacaaag
     9361 tggttttgga agtcccttcc ccacaagccg gcgtattggt tgaaatcgta gcgcaagacg
     9421 gtgaaaccgt tgttgccgac caagttttgg cacgcatcga tacagctgct actgccgctg
     9481 ccgaagcacc cgtagccgct cctgccgaag ccgccccggc tgccgctcct gctgccgcac
     9541 aaaacaacgc cgctatgcct gccgccgcca aactggctgc ggagaccggt gttgacgtga
     9601 acgcattgca aggttccggc cgcgacggtc gcgtattgaa agaagacgta caaaatgccg
     9661 ctgccaaacc tgccgcagcc gctgctcctg ctgttgcact tcctgccggc gcacgtcctg
     9721 aagaacgcgt accaatgagc cgcctgcgtg cccgtgttgc agaacgcctc ttggcttctc
     9781 aacaagaaaa cgccattctg actacattca acgaagtcaa catgaaacct atcatggact
     9841 tgcgtgcgaa gtacaaagaa aaatttgaga aagaacacgg cgtgaaattg ggctttatgt
     9901 ccttcttcgt taaagccgct gttgccgccc tgaaaaaata cccggttgtg aatgcttctg
     9961 ttgacggcaa agacatcgtt taccacggct acttcgacat cggtatcgcg attggcagcc
    10021 cacgcggttt ggttgtgcca atcctgcgcg atgccgacca aatgagcatt gcagacatcg
    10081 aacaagcaat tgttgattac gcgaaaaaag ccaaagacgg caaaatcgct atcgaagatc
    10141 tgaccggcgg tacattcagt attaccaacg gcggtacttt cggttctatg atgtctaccc
    10201 cgatcatcaa cccgcctcaa tctgcaattt tgggtatgca cgccactaaa gagcgcgctg
    10261 tggttgaaaa cggtcaagtt gttgtccgtc caatgatgta tctggctctg tcttacgacc
    10321 accgtatcat tgacggccgc gaagctgtat tgaccttggt agccattaaa gacgcgttgg
    10381 aagacccagc ccgcctgttg ttggatctgt aatcgtttca gacggccttt tatttgttaa
    10441 tgaaaaggcc gtctgaa
//
LOCUS       DAWHSK010000043         9916 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.043, whole genome shotgun sequence.
ACCESSION   DAWHSK010000043 DAWHSK010000000
VERSION     DAWHSK010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9916
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.043"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            261..1562
                     /gene="bioA"
                     /locus_tag="WIC03_000734"
     CDS             261..1562
                     /gene="bioA"
                     /locus_tag="WIC03_000734"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HEZ3883268.1"
                     /translation="MPSEHQHTSSLLDFDRTHLLHPYTSMTDPLPVYPVKRAEGVFIE
                     LADGTRLIDGMSSWWCAIHGYNHPVLNQAVEAQIKQMSHVMFGGLTHDPAVELGKLLV
                     GILPQGLDRIFYADSGSVSVEVALKMAVQYQQARGLTAKQNIATVRRGYHGDTWNAMS
                     VCDPETGMHHIFGSALPQRYFVDNPKSRFDDEWDGADLQPVRALFEVHHADIAAFILE
                     PVVQGAGGMYFYHPQYLRGLRDLCDEFDIVLIFDEIATGFGRTGKMFACEHAEVVPDI
                     MCIGKGLSGGYMTLAAAITSQKVTETISRGEAGVFMHGPTFMANPLACAVACASVKLL
                     LSQDWQANIRRIESILKGRLKAAWDIRGVKDVRVLGAIGVIELDKGVDMARFQADCVA
                     QGIWVRPFGRLVYLMPPYIISDGVLTKLADKTVQILKEHSK"
     gene            1559..2206
                     /gene="bioD"
                     /locus_tag="WIC03_000735"
     CDS             1559..2206
                     /gene="bioD"
                     /locus_tag="WIC03_000735"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HEZ3883269.1"
                     /translation="MKGVYFVSGIDTDIGKTVATGVLAKQLLQQGKSVITQKPVQTGC
                     QNIADDIAVHRKIMGKPMQEADKQGLTMPEIFSYPASPHLAARLDGRALDLDKIRTAT
                     QELAAQYEVVLVEGAGGLMVPLTENLLTIDYIRQQGYPVILVTSGRLGSINHTLLSFA
                     ALKQYGIRLHSLVFNHIHDSRDAHIAQDSLSYLKCRLKADFSEAEWMELAKTDAV"
     gene            2221..2697
                     /locus_tag="WIC03_000736"
     CDS             2221..2697
                     /locus_tag="WIC03_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HEZ3883270.1"
                     /translation="MEHLFGKWLPDLPAAISDGISLPMVRLLHTRSLTAALRALPHTF
                     SVELLKLGELETECGGRLVREVLLKLDRIPVVEARSECRIGSAFWQNILDCGTRPLGE
                     RLFQADLEGARSVFEFAVAGEGCGRYFAARRSRFSHQGEEMLLTEYFLPELKRFIG"
     gene            2727..3617
                     /gene="ubiA"
                     /locus_tag="WIC03_000737"
     CDS             2727..3617
                     /gene="ubiA"
                     /locus_tag="WIC03_000737"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HEZ3883271.1"
                     /translation="MNPKSPLFLRLSDRLDVYLRLMRADKPIGTLLLLWPTYWALWLA
                     SDGIPDLAVLAAFTIGTFLMRSAGCVINDFADRDFDGAVERTKNRPFAQGRVKKKEAL
                     LLTAFLCLLAALCLIPLNHLTWLMSLPALFLALTYPFTKRFFPIPQLYLGLAFSFGIP
                     MAFAAVAGNVPPQAWILFAANVLWTLAYDTVYAMADKEDDLKIGIKTSAVTFGRYDIA
                     AVMLCHGGFTLLMAVLGAVIGAAWAYWTAIPIVLLLQYRQYAAIKSRVRQICFETFLA
                     NNRIGWVWFTAIFAHTFFAK"
     gene            3802..4251
                     /gene="ptsN"
                     /locus_tag="WIC03_000738"
     CDS             3802..4251
                     /gene="ptsN"
                     /locus_tag="WIC03_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HEZ3883272.1"
                     /translation="MSLIGEILPLSHIVLDMEVGSKKRLFEEAGLLLERESSLSHADV
                     FECLFAREKLGSTGLGQGVAIPHGRHAGVKQATGAFIRTREPVGFDAPDGKPVSLIFI
                     LLVPENATGEHLEVLSKLAGKFSQKSIRESLMTVSSAEEARAILTEE"
     gene            4255..5217
                     /locus_tag="WIC03_000739"
     CDS             4255..5217
                     /locus_tag="WIC03_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEZ3883273.1"
                     /translation="MPSISVRRLFDDNQYKLQLAWAAGNSGADNRIGVEADKPVLALV
                     GHLNFIHPNQIQVVGLAESEYLNRLESGETGYQFGDLFDISMSLVIVANGLPVSPGLR
                     DYCHKNDIPLLTSKLESPYLMDVLRIYLQRTLAASSVKHGVFLDVFEIGVLITGHSGL
                     GKSELALELISRGHSLIADDAVELFRIGPETLEGRCSPMLRDFLEVRGLGILNIRHIF
                     GETSIRPKKILQLIINLVEADDEYMKQLDRLSIRTETESILNVNVRSVTLPVAVGRNL
                     AVLVEAAVRNYILQLRGKDSTREFLERHQTQLKENEQNHENRPD"
     gene            5198..6052
                     /gene="rapZ"
                     /locus_tag="WIC03_000740"
     CDS             5198..6052
                     /gene="rapZ"
                     /locus_tag="WIC03_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEZ3883274.1"
                     /translation="MKIVLISGLSGSGKSVALRQMEDSGYFCVDNLPLEMLPALVSYH
                     IERADETELAVSVDVRSGIDIGQAREQIAYLRGLGHRVEVLFVEAEESVLVRRFSETR
                     RGHPLSNQDMTLLESLKKEREWLFPLKEIAYCIDTSKMNAQQLRHAVRQWLKVERTGL
                     LVILESFGFKYGVPNNADFMFDMRSLPNPYYDPELRPYTGMDKPVWDYLDGQPLVQEM
                     VDDIERFVTHWLPRLEDESRSYVTVAIGCTGGQHRSVYIVEKLARRLKGRYELLIRHR
                     QAQNLPSR"
     gene            6134..7075
                     /gene="ylqF"
                     /locus_tag="WIC03_000741"
     CDS             6134..7075
                     /gene="ylqF"
                     /locus_tag="WIC03_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEZ3883275.1"
                     /translation="MAIQWFPGHMNKAKKAIAERAKSVDMVIEMLDARMPASSENPLL
                     AQLSKGKPKLKILNKQDLADPERTKVWLDHYNSRPDTRAIALDSSETGAHGKITQACR
                     AMIPHRQGIDKPLRVLICGIPNVGKSTLINGMIGKKSAKTGNEPGITKAEQRLFLADD
                     FWLYDTPGMLWPKIIVEESGYNLAAGGSVGRNALDEEEVALELLDYLRRHYLPMLQER
                     YQADKDPSSHWDENVWLEWIAKKRGAVLSGGRINYQKAAENILTDFREGKIGRITLET
                     PNQWETWLKKARQKEAELKAIREARKAERKGQKPADE"
     gene            complement(7166..7286)
                     /locus_tag="WIC03_000742"
                     /pseudo
     CDS             complement(7166..7286)
                     /locus_tag="WIC03_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            7350..7952
                     /locus_tag="WIC03_000743"
     CDS             7350..7952
                     /locus_tag="WIC03_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEZ3883276.1"
                     /translation="MTEKLPPDALIEAALLTQAEPLNEKTMRELCVPPLSQDKLIDVL
                     AQLKTRWQDRALQLVHTQEGWRFQIVQTAFERLGSLQEQRAPRYSRAVMETLAIIAYQ
                     QPVTRGDIEGIRGVAVSQNVMQTLQDRGWIEVIGHRDTLGKPALWATTATFLSDLRLD
                     GLEELPPLTELGELVLPDLMEMPPTDEEEPEAVPSDTLPN"
     gene            8136..9809
                     /gene="recN"
                     /locus_tag="WIC03_000744"
     CDS             8136..9809
                     /gene="recN"
                     /locus_tag="WIC03_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEZ3883277.1"
                     /translation="MLLTLSLRDFVIVENLNLDFQSGFTVLTGETGAGKSITLDAIGL
                     LLGDKADYSQVRSGAKEAQLSALFDISHLPVLKAELYEQGLLNDGEEELSIRRIIDAK
                     GKSRSFINNQAATLAQLKSVGSQLIDIHGQNAHHSLNQEAAQRELLDAFAGSREQAET
                     VRQLYQNWANAKKALQEAQEHADAVIIERERLEWQFNELNQLDIKQGEWEALSQSHDS
                     LAHSAELLQAAEEVGSKIDGDNGIQRHIYQCQKLLANLQNIEPRFAESLNMLASIEAE
                     LGEISANMRDVAGRSDINPNELAAQEQRMGELMGMARKYRIEPEELPAKLAEIEERLQ
                     SLQAAADLDALEHNVAHNFAEYQEAAHILSAMRHQAAERLSGETTEHMQHLAMKGARF
                     DIVLLPSSPTAHGLEQVQFQVAANKGNPPRPLNKVASGGELARISLALQVVASQYTQV
                     PTLIFDEVDTGIGGGVAEMVGKALRALGRKHQVLAVTHLPQVASCGENHWRVRKHSEG
                     EQTVSEISILDEIQRIEEVARMLGGEVITDTTRQHAAELLQLASKNSLF"
BASE COUNT         2428 a         2492 c         2691 g         2305 t
ORIGIN      
        1 tccttgtcct gatttaaatt taatccacta tatctatgac tgattgaagc gttgggcgga
       61 ggctgcgtga aacggtattg ggcgttgggc cgtctgattc caatcgggct tgaggaatgc
      121 gaaacggtgt gcgcttatgt tgcggaacga tttgtttcgc ggttttgcgc ccgaaacgga
      181 tggaaaggtg tgggaagcgg tctgtcggag tagaatacgc gtttgcgttt gaatacagta
      241 agaagaaaag agagaaactt atgccgtctg aacatcaaca cacatcatca ttgcttgatt
      301 tcgaccgtac ccatctgctt catccctata cttccatgac cgatccgctt cccgtttatc
      361 ctgtcaaacg tgcagaaggg gtgtttatcg aattggcgga cggcacacgg ctgattgacg
      421 ggatgtcgtc ttggtggtgt gcgatacatg gttacaatca tcctgttttg aatcaggcgg
      481 ttgaggcgca gattaagcaa atgtcgcatg taatgtttgg cggtttgacg cacgatcctg
      541 cggtggaact gggcaagttg ttggtcggaa ttttgccgca ggggctggac cgtatttttt
      601 atgcggattc gggttcggtt tcggtggaag tcgcgttgaa aatggcggtg caataccagc
      661 aggcgcgggg gttgacggcg aagcagaata ttgcgacggt gcggcgcggg tatcacggcg
      721 atacttggaa cgcgatgtcc gtctgcgatc cggaaacggg gatgcaccat attttcggca
      781 gcgcgttgcc gcagcgttat tttgtcgata atccgaaaag ccgtttcgac gatgaatggg
      841 acggggcgga tttgcagcct gtccgcgcct tgtttgaagt gcatcatgcg gatattgccg
      901 cctttatttt agagccggtc gtgcagggcg cgggcggcat gtatttttat catccgcagt
      961 atcttcgcgg attgcgcgat ttgtgcgacg agtttgatat cgttttgatt tttgatgaaa
     1021 tcgccaccgg attcgggcgc acgggcaaga tgtttgcctg cgaacacgcg gaggtcgtgc
     1081 cggatattat gtgtatcggc aagggcttga gcggcggcta tatgacgctg gcggcagcaa
     1141 tcacttcgca aaaagttacc gaaacgattt cgcgcggcga agcgggcgtg tttatgcacg
     1201 gcccgacgtt tatggcaaac ccgctggcgt gtgccgttgc ctgcgcttcg gtcaaactgc
     1261 ttttgtctca agactggcag gcaaatatcc gccgcattga aagcatctta aaaggccgtc
     1321 tgaaagccgc gtgggacatt cgcggcgtga aagacgtacg cgttttgggt gccatcggcg
     1381 tgatcgagct ggataaaggc gtggatatgg cgcgttttca agcagattgc gtggcgcagg
     1441 gcatttgggt gcgcccgttc ggcaggctgg tgtatctgat gccgccgtat attatttcag
     1501 acggcgtttt gaccaaactt gccgacaaaa ccgtgcaaat cttgaaggaa cacagcaaat
     1561 gaaaggcgtt tacttcgtca gcggcataga cacggacatc ggcaaaaccg tcgccaccgg
     1621 cgtgttggca aaacaattgt tgcagcaggg caaaagcgtg attacgcaaa agcccgtgca
     1681 aaccggttgc caaaacattg ccgacgacat agccgtccat cgtaaaatca tgggtaaacc
     1741 catgcaggaa gccgacaaac aagggctgac catgcctgaa atcttcagtt atccagcttc
     1801 gcctcatctc gctgcccgtc ttgacggcag ggctttggac ttggacaaaa tccgcaccgc
     1861 cacacaagaa ttggcggcgc agtacgaagt cgttttggtc gaaggcgcgg gcggattgat
     1921 ggttccgctg acggaaaacc tgttaaccat tgattatatc cgtcagcaag gctatcccgt
     1981 catcctcgtt accagcggac ggctcggcag tatcaaccac actttactca gtttcgccgc
     2041 gctcaaacaa tacggcattc gcttgcacag cctcgtgttc aaccacatcc acgacagccg
     2101 cgacgcacac atcgcccaag acagcctgag ctacttgaaa tgccgtctga aagccgattt
     2161 ttccgaagcg gaatggatgg agttggcaaa aacagacgcg gtataaagat tgggaaaaat
     2221 atggaacacc tatttgggaa atggctgccc gacttgcccg ccgccatttc agacggcatc
     2281 agcctgccga tggtgcggct gctgcacacc cggtcgctga ccgccgcatt gcgcgccttg
     2341 ccgcatacat tttcggtgga actgctgaaa ctgggcgaat tggagacgga atgcggaggg
     2401 aggctggtgc gcgaagtttt gttgaagctg gaccgtatcc ctgttgttga ggcaaggagc
     2461 gaatgccgta tcggttcggc gttttggcaa aacattttgg actgcggcac gcgtcctttg
     2521 ggcgagcgtc tgtttcaagc cgatttggaa ggggcgcgtt cggtgtttga gtttgccgtt
     2581 gccggcgaag gatgcggacg gtactttgcc gcgcggcgtt ctcggttttc ccatcaaggc
     2641 gaggaaatgc tgctgaccga gtattttctg cccgaactga aacgctttat cggataaaat
     2701 accgtttttt caagctgtgc ggcaatatga atcctaaatc ccctttattt ttacgactgt
     2761 ccgaccgttt ggatgtgtac ctgcgcctga tgcgggcgga caagcccatt gggacgctgc
     2821 ttttgctgtg gccgacctac tgggcgttgt ggctggcttc agacggtatt cccgatttgg
     2881 cggtattggc ggcgtttaca atcggcacgt ttttaatgcg cagtgccggc tgcgtcatca
     2941 acgattttgc cgaccgcgat tttgacgggg ctgtcgagcg cactaaaaac cgtccgttcg
     3001 cacagggcag ggtcaagaaa aaagaagcgc tgctgctgac ggcatttttg tgcctgcttg
     3061 ccgcattgtg cctgattccg ctgaatcatc tgacatggct gatgagtctg cccgcgctgt
     3121 ttcttgcgct gacttaccca tttaccaaac gcttttttcc gattccccaa ctctatctcg
     3181 ggcttgcctt ttccttcggt atcccgatgg cgtttgccgc cgttgccgga aacgtgccgc
     3241 ctcaagcgtg gatactcttt gccgccaatg tgttatggac tctggcgtat gacacggttt
     3301 atgcaatggc ggacaaagaa gacgatttga aaatcggcat caaaacctcc gccgtcacgt
     3361 tcgggcgtta cgacatcgcc gctgttatgc tgtgtcatgg cggctttacc ctgctgatgg
     3421 cagtattggg ggcggttatc ggtgcggcat gggcatattg gacggcaatc cccatcgtcc
     3481 tgctgctgca ataccgccaa tatgccgcca tcaaaagccg cgtccggcaa atctgttttg
     3541 aaacgttttt ggcaaacaac agaattggtt gggtgtggtt taccgccatt tttgcccata
     3601 cgtttttcgc gaaataaggc agggcaatgc cgtctgaaga gccgtaaact gctttggacg
     3661 gcatttctat ctgtgccgaa aagcgttaaa atatgttttt aaaacgctgt gttatgtcag
     3721 cccgtaccgt atgcgggatt gagatttgcc ccggcagtcg gtacaatctt tctgttttgc
     3781 gatgtctgaa aagagaagct tatgagcctt atcggcgaaa ttttgccttt gtcccatatt
     3841 gttttggata tggaggtggg cagtaaaaaa aggctgtttg aggaagcagg cctgcttttg
     3901 gaacgcgaat cctcattgtc ccatgctgat gttttcgaat gtctttttgc ccgtgaaaaa
     3961 ctcggttcga ccggtttggg gcagggcgtt gccatcccgc acggccgtca tgccggcgtg
     4021 aagcaggcga cgggcgcgtt catccgcacg cgcgaacccg tcggatttga cgcaccggac
     4081 ggcaaaccgg tttccctgat ttttatcttg ctggttcctg agaatgcaac gggcgagcat
     4141 ttggaggtat tgtccaaact ggccggcaag ttttcccaaa aaagcatccg cgaatcgctg
     4201 atgacggttt cttctgcgga agaagcccgc gccatcctga ccgaagaata aaatatgccc
     4261 agtatctctg tccgccgact gtttgacgac aaccaataca agttgcaact tgcctgggcc
     4321 gccggcaatt cgggtgcgga caaccgtatc ggcgtagagg cggacaagcc cgtcctcgct
     4381 ttggtcggac acctgaattt catccacccc aaccaaatcc aagtggtcgg tttggcggag
     4441 tcggaatatc tgaaccgtct cgaatcgggg gaaacgggtt atcagttcgg cgacctgttc
     4501 gacatttcta tgtctttggt tattgtggca aacggcttgc cggtttcccc gggactgcgc
     4561 gactactgcc ataaaaacga tattccactg ctgacttcca aactcgaaag cccctatctg
     4621 atggacgtgt tgcggattta cctgcaacgc accctagcgg catcgtccgt caaacacggc
     4681 gtatttctcg atgtgtttga aatcggcgtg ctgattaccg gacattcggg tttgggtaag
     4741 agcgaattgg cgttggagct gatttcgcgc ggccacagcc tgattgccga cgatgcggtc
     4801 gagctgttcc gcatcggccc ggaaacgctg gaagggcggt gttcgcctat gctgcgcgat
     4861 tttttggaag tgcgcggctt ggggatactc aatatccgcc atattttcgg cgaaacttcc
     4921 atccgcccca aaaaaatcct gcaactcatt atcaatttgg tcgaggcaga cgacgagtat
     4981 atgaagcagc tcgaccggtt gagtatccgc accgaaaccg aatccatcct caacgtcaac
     5041 gtccgttcgg taacgctgcc tgtcgcggtc ggacgcaacc ttgccgtatt ggttgaagcg
     5101 gcggtacgca attacatttt gcagttgcgc ggtaaggaca gtacgcgcga atttttggaa
     5161 cgccatcaga cgcaacttaa agaaaacgaa cagaatcatg aaaatcgtcc tgattagcgg
     5221 gttgtccggt tcgggcaaat ccgtcgcact gcgccaaatg gaagattcgg gttatttctg
     5281 cgtggacaat ttgcctttgg aaatgttgcc cgcgctggtg tcgtatcata tcgagcgtgc
     5341 ggacgaaacc gaattggcgg tcagcgtcga tgtgcgttcc ggcattgaca tcggacaggc
     5401 gcgggaacag attgcctatc tgcgcggact ggggcacagg gttgaagttt tgtttgtcga
     5461 ggcggaagaa agcgtgttgg tccgccggtt ttccgagacc aggcgaggac atcctctgag
     5521 caatcaggat atgaccctgt tggaaagctt aaagaaagaa cgggaatggc tgtttccgct
     5581 taaggaaatc gcctactgta tcgacacttc caagatgaat gcccaacagc tccgccatgc
     5641 agtccggcag tggctgaagg tcgaacgtac cgggctgctg gtaattttgg agtccttcgg
     5701 gttcaaatac ggtgtgccga acaacgcgga ttttatgttc gatatgcgca gtctgcccaa
     5761 cccgtattac gatcccgagt tgaggcctta caccggtatg gacaagcccg tttgggatta
     5821 tttggacgga cagccgcttg tgcaggaaat ggttgacgac atcgaaaggt ttgttacgca
     5881 ttggttaccg cgtttggagg atgaaagcag gagctacgtt accgtcgcca tcggttgcac
     5941 gggaggacag caccgttcgg tctatattgt cgaaaaactc gcccgaaggt tgaaagggcg
     6001 ttatgaattg ctgatacggc acagacaggc gcaaaacctg ccgagccgtt aatcccatta
     6061 aacaattatg ccgtctgaaa ccccgctttt ctgtttcaga cggcatttca aatattgaga
     6121 agaaaggaaa gatatggcaa tccaatggtt tcccggtcat atgaacaagg cgaaaaaagc
     6181 catcgccgag cgtgcaaaaa gcgttgatat ggtgattgaa atgctggacg cgcgtatgcc
     6241 cgcctccagc gaaaaccccc tgcttgccca gctttccaaa ggcaaaccca aacttaaaat
     6301 cctaaacaaa caagatcttg ccgaccccga acgcacaaag gtctggcttg accactacaa
     6361 cagccgcccc gacacgcgcg ccattgccct cgattcctcc gaaaccggcg cacacggcaa
     6421 aatcacccaa gcctgccgcg cgatgattcc ccaccggcaa ggcatagaca aacccttgcg
     6481 cgtcttgatt tgcggcattc ccaacgtcgg caaatccacc ctcatcaacg gcatgatagg
     6541 caaaaaatcc gccaaaaccg gcaacgaacc cggcatcacc aaagccgaac aacgcctttt
     6601 ccttgccgac gacttctggc tgtacgacac ccccggaatg ctgtggccga aaatcattgt
     6661 cgaagaaagc ggctacaacc tcgccgccgg cggttcagtc ggacgcaacg cattggacga
     6721 agaagaagtc gccctcgaac ttttagacta cctccgccgc cactacctcc ctatgttgca
     6781 agaacgctac caagccgaca aagaccccag cagccactgg gacgaaaacg tttggctcga
     6841 atggatagcc aaaaaacgcg gcgcagtcct cagcggcgga cggatcaact accaaaaagc
     6901 cgccgaaaac atccttaccg acttccgtga aggcaaaatc ggcagaatca ccctcgaaac
     6961 gccgaaccaa tgggaaactt ggctcaaaaa agcccgtcag aaagaagccg aactcaaagc
     7021 catacgcgaa gccagaaaag cagagcgtaa agggcagaag cctgcggacg agtagggcgg
     7081 tatagtggaa tgccaaaaga ggtttcacta tcttcaaaca ggtcgtctga aacctcagaa
     7141 taaatttttc agacgacctg tttgtaaaat ttaaaaccaa catatatagt ggattaactt
     7201 taaaccagta cggcgttgcc ttgccgtact atctgtactg tctgcggctt cgtcgccttg
     7261 tcctgattta aagttaatcc actataaaac tgaaagaacc cttatccaag gctgtctgaa
     7321 aaatatttaa actattaaat ccggatgtta tgactgaaaa actccctcct gatgcgctga
     7381 ttgaagccgc gcttttgact caagccgaac ctttaaacga aaagaccatg cgcgaattgt
     7441 gtgtgccgcc gttgtcgcaa gacaaactga ttgatgtgtt ggcgcagttg aaaacgcgtt
     7501 ggcaggatag ggcgttgcaa ctggtgcata cgcaagaggg ctggcgtttt cagattgttc
     7561 agacggcatt cgagcggctg ggcagcctgc aagaacagcg tgcgccgcgc tactcccgcg
     7621 ccgtgatgga aacactggcg attatcgcct accagcagcc cgtaacgcgc ggcgacatcg
     7681 agggcatacg cggcgtggcg gtgtcgcaga acgtgatgca gaccttgcag gatcgggggt
     7741 ggattgaagt catcggacat cgggacacat tgggaaaacc cgcattgtgg gcaacaacgg
     7801 caacgtttct cagcgatttg cgcttggacg gtctggaaga acttccgccg ctgaccgaac
     7861 tgggcgaact ggttttaccc gatttaatgg aaatgccgcc tacggatgaa gaagagccgg
     7921 aagccgtacc gtccgatacc ctgcccaact gaaattccaa atgccgtctg aaacgcacat
     7981 tgcttcagac ggcattgcaa caaataagca gataaaaaca agtactaagc aaaattaagg
     8041 aaaacttatt tttaatttaa aaaaatctta gttataattc gtatatctaa agttgatatt
     8101 gcttttgtcg gtagaattgc taaggaatcc tcacaatgct tctaacactt tctttgcgtg
     8161 attttgtcat tgttgaaaat ctgaatctgg attttcaaag cggctttacc gtattgaccg
     8221 gagaaaccgg cgcgggcaag tccattactt tggatgcgat tggtctgctg ttgggcgaca
     8281 aagccgatta cagccaagtc cgcagcggtg caaaagaagc gcagttgtcg gcattgttcg
     8341 atatttccca tttgcctgtt ttaaaagcag aattgtatga acaggggctt ttaaacgacg
     8401 gagaagaaga actcagtatc cgccgcatta tcgatgccaa aggcaaaagc cgcagcttta
     8461 tcaacaatca ggccgctacc ttggcgcaac tcaaatccgt cggtagccag cttatcgaca
     8521 tccacgggca aaacgcccat cattcgctta atcaggaagc cgcccagcgc gaattgttgg
     8581 acgcatttgc gggtagcagg gagcaggcgg aaaccgtcag gcagctttat caaaattggg
     8641 ccaatgcgaa aaaagccctc caagaggcgc aggaacacgc cgatgccgtc attatcgagc
     8701 gggagcgtct ggaatggcag tttaacgaac tgaatcagtt ggacattaaa caaggcgaat
     8761 gggaagccct cagccaaagc cacgacagcc ttgcccattc tgccgagctg ttgcaggctg
     8821 ccgaagaagt cggaagcaag attgacggcg acaacggcat ccaacgccat atctatcaat
     8881 gtcaaaaact attggccaat ctgcaaaaca tcgagccgcg ctttgccgag agcctgaata
     8941 tgttggcaag catcgaagcc gaattgggcg aaatcagtgc caatatgcgc gatgtggcag
     9001 gtcgcagcga catcaatccc aatgaattgg cggcacagga acagcgcatg ggcgaactga
     9061 tggggatggc gcggaaatac cggatcgagc ctgaagagtt gcctgccaag ttggcagaaa
     9121 tcgaagaacg cctgcaaagc ctgcaagctg ccgccgattt ggacgcgctc gagcataatg
     9181 ttgcccacaa ttttgccgaa tatcaggaag ctgcccacat cctttctgcc atgcgccatc
     9241 aggcggcaga gcgtttgagc ggcgaaacga ccgagcatat gcaacacctt gccatgaaag
     9301 gtgcacgttt cgacatcgtc ctgttgccct cgtcgccgac ggcacacggt ttggagcagg
     9361 ttcaatttca agttgccgcc aacaaaggca atccgccccg tccgctgaat aaagttgcct
     9421 ccggcggcga attggcgcgt atcagccttg ccttacaggt tgttgccagc caatataccc
     9481 aagttcccac cctgattttt gatgaggtcg ataccggtat tggaggggga gtggctgaaa
     9541 tggtcggcaa ggcattacgt gcgttgggca gaaaacatca ggtgcttgcc gttacccacc
     9601 ttccccaagt cgcatcctgc ggagaaaacc actggcgggt gcgcaagcac agcgagggag
     9661 agcaaaccgt cagcgaaatc agtatattgg atgaaatcca acggatcgaa gaggttgccc
     9721 gtatgttggg cggagaagtc attaccgata cgacgcggca acatgcggca gaattgctgc
     9781 aacttgcgtc gaaaaatagt ttattttaaa atcaatcagt taaaaaataa ctaaaaataa
     9841 aagtctaaaa caatagacag aactcagata aatccgtatt atcacgcttt cttaatcact
     9901 tgaacaagtg attgtg
//
LOCUS       DAWHSK010000044         9794 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.044, whole genome shotgun sequence.
ACCESSION   DAWHSK010000044 DAWHSK010000000
VERSION     DAWHSK010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9794
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.044"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(10..1305)
                     /locus_tag="WIC03_000745"
     CDS             complement(10..1305)
                     /locus_tag="WIC03_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEZ3883278.1"
                     /translation="MIRPDFQEYLPSYYFSSVNPHTVYPKLQCRLKTDTCIIGGGLSG
                     LCTALPLAEQGHETVVLEAARIGFGASGRSGGQVISDYACGMGEIEKQVGLEQAQWFW
                     QQSLQAVELVDERVRKHAIDCDWQRGYATVAVRPQHWEELQQWHEHAQRHYGASHYQL
                     WDKAELKQQLDSDMYQGAQFDPLSGHLHPLNYTLGIARAAAEAGAQIFEQSPMTCIEP
                     HQNGWLVYTPEGSVECKNVVYAVNTYAGLNPIFKPLERKAIAVSTFIIATEPLGARAK
                     GLIRNNMAVCDNRHILDYYRLSADGRLLFGGKDNEFIDNPARMTELVRQDMLKVFPQL
                     ADVKIEYSWGGECDITANLVPHFGRLAPNVFYTQGYSGHGMAITGIAGLAVAEAILGD
                     ECRLKPFEQLRQPNIILQPFLRKLGSFLGSKYYQWKDSR"
     gene            complement(1431..2318)
                     /locus_tag="WIC03_000746"
     CDS             complement(1431..2318)
                     /locus_tag="WIC03_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEZ3883279.1"
                     /translation="MQKSKLSWFLKLMLALSLAFLYIPLVVLVIYSFNESKLVTVWGG
                     FSTKWYGALLENDTILEAAWLSLRIAVVSSLAAVVLGTLAGYAMARIKRFRGSTLFAG
                     MISAPMVMPDVITGLSMLLLIIQVQIFLQGSEWLQHLYFDRGFFTIFLGHTTLCMAYI
                     TVVIRSRLVELDQSLEEAAMDLGARPLKIFFVITLPLIAPAIASGFLLGITLSLDDLV
                     ITSFLSGPGSSTLPQVIFSKIKLGLDPQMNVLATILIGIIGTLVIIVNYWMMRQATRR
                     DREAAEAYRQEKLAAEKAH"
     gene            complement(2318..3283)
                     /locus_tag="WIC03_000747"
     CDS             complement(2318..3283)
                     /locus_tag="WIC03_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEZ3883280.1"
                     /translation="MNLNKLKNKLFRRPGQRVVIAVPYIWLLVLFLIPFAIVLKISFA
                     EQEIAIPPFTPLTSIDEDLGRLNITISYQNYADIFQNFWHTLNPFGDSENSNIYLMTY
                     WSSIKTALMTTVICLLVGYPTAYAISRANPSVRNGLLLAIMLPFWTSFLLRVYAWMGL
                     LGHNGIVNNLLIKMGIISEPLDLFYNAFSLNLVMVYAYLPFMILPLYTQLVKLDNRLL
                     EAASDLGAGPVKSFLTITLPLSKTGIIAGSMLVFVPAVGEFVIPELVGGSENLMIGKV
                     LWQAFFDQNNWPLASAVAVVMVALLVVPIALFQHYENRELEEGAK"
     gene            complement(3299..4423)
                     /gene="potA"
                     /locus_tag="WIC03_000748"
     CDS             complement(3299..4423)
                     /gene="potA"
                     /locus_tag="WIC03_000748"
                     /EC_number="7.6.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter ATP-binding protein"
                     /protein_id="HEZ3883281.1"
                     /translation="MTATTASSAKPYLKIQGLVKKFGDNYAVDNIDLDIYQHEIFALL
                     GSSGSGKSTLLRMLAGMESPNQGKIILDGQDITKLAPYDRPINMMFQSYALFPHMTVE
                     QNIAFGLKQDKMPKGEIAARVEEMLRLVQMTKFAKRKPHQLSGGQQQRIALARSLAKR
                     PKILLLDEPLGALDKKLRQQTQLELVNTLEQVGVTCIMVTHDQEEAMTMATRIAIMSD
                     GQLQQVGTPSDVYDYPNSRFTAEFIGETNIFDGVVIEDHADYAVIECEGLENHVRIDH
                     GLGGPSEQDLWVSIRPEDIDLYKEKPEYLGDYNWAKGTVKEIAYLGSFAIYHIKLANG
                     RVVKSQVPAPYWYVRNITPPTWDETVYISWPENQPTPLFR"
     gene            complement(4618..4725)
                     /locus_tag="WIC03_000749"
                     /pseudo
     CDS             complement(4618..4725)
                     /locus_tag="WIC03_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(4804..5073)
                     /gene="rpsO"
                     /locus_tag="WIC03_000750"
     CDS             complement(4804..5073)
                     /gene="rpsO"
                     /locus_tag="WIC03_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEZ3883282.1"
                     /translation="MALTVEQKAQIVKDFQRKEGDTGSSEVQVALLTFRINDLTPHFK
                     ANPKDHHSRRGLLKMVSQRRRLLAYLRRTQPDTYRALITRLGLRK"
     gene            complement(5296..6231)
                     /gene="secF"
                     /locus_tag="WIC03_000751"
     CDS             complement(5296..6231)
                     /gene="secF"
                     /locus_tag="WIC03_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEZ3883283.1"
                     /translation="MELFKIKRDIPFMSYGKLTTFISLVTFIAAVFFLVTKGLNFSVE
                     FTGGTVMEVQYQQGADVNKMRERLDTLKIGDVQVQALGTNKHIMIRLPNKEGVTSAQL
                     SNQVMDLLKKDSPDVTLRQVEFIGPQVGEELVSNGLMALGFVVIGIIIYLSMRFEWRF
                     AVSAIIANMHDIVIILGCFAFFQWEFSLTVLAGILAVLGYSVNESVVVFDRIRENFRK
                     PAMRGHAVPEVIDNAITATMSRTIITHGSTEAMVVSMLVFGGAALHGFSMALTIGIVF
                     GIYSSVLVASPLLLMFGLSRDNIGKEPKKKEEIVV"
     gene            complement(6235..8088)
                     /gene="secD"
                     /locus_tag="WIC03_000752"
     CDS             complement(6235..8088)
                     /gene="secD"
                     /locus_tag="WIC03_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEZ3883284.1"
                     /translation="MNRYPLWKYLLIVFTIAVAAVYSLPNLFGETPAVQVSTNRQAII
                     INEQTQFKVDAALKNAGIQTDGMFVVDNSLKVRFKDTETQLKARDVIENTLGEGYITA
                     LNLLADSPEWMAKIKANPMFLGLDLRGGVHFTMQVDMKAAMQKTFERYSGDIRRELRR
                     EKIRSGTVRQAGNSLTVPLQDAGDVQKALPQLRKLFPEATLNSDGSNIVLTLSEEAVN
                     KVRSDAVKQNITTLHNRVNELGVAEPVIQQSGADRIVVQLPGVQDTAKAKDIIGRTAT
                     LELRMVEDDPAKLREALEGNVPSGYELLSSGGDRPETLLISKQVELTGDNINDAQPSF
                     DQMGAPAVSLSLDSAGGSIFGELTAANVGKRMAMVLIDQGKSEVVTAPVIRTAITGGR
                     VEISGSMTTAEANDTSLLLRAGSLAAPMQIVEERTIGPSLGKENIEKGFHSTLWGFAI
                     VAAFMVVYYRLMGFFSTIALSANILFLIGILSAMQATLTLPGMAALALTLGMAIDSNV
                     LINERIREELRAGVPPQQAINLGFQHAWATIVDSNLTSLIAGIALLVFGSGPVRGFAV
                     VHCLGILTSMYSSVVVFRALVNLWYGRRRKLQNISIGSVWKPKAEMAGGKE"
     gene            complement(8300..8566)
                     /gene="yajC"
                     /locus_tag="WIC03_000753"
     CDS             complement(8300..8566)
                     /gene="yajC"
                     /locus_tag="WIC03_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEZ3883285.1"
                     /translation="MNQAVAQFAPLVLIMVVFYFLIMRPQQKKFKAHQAMLAALKVGD
                     KVVLAAGFKGKVTRVGEQFFTVDIGQSTKIEVEVERNAISAKVD"
     gene            8694..>9794
                     /locus_tag="WIC03_000754"
     CDS             8694..>9794
                     /locus_tag="WIC03_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEZ3883286.1"
                     /translation="MSLTRLILKFYALLRLFLGKNARTAWISHPACAGHEPGANHPDS
                     PDRILCIEQALRRAGIWQHLQTIEAEEISDTRLALVHSSKYLNRLESCQPQKGKIFRL
                     DDDTAISTGSLSAARFAAGSAVQAVDMVMNRKAWHAFCAARPPGHHAGSGKAGGFCLL
                     NNVAAGVMHAIAEYRLKRIAVIDFDVHYGDGTAEIFKDDPRILFFNLFETDLFPFPEN
                     NDMPDGGNMVHLPLPPGTGSRTFREAVRRQWLPRLAAFKPELVLLSAGFDAHRLDESG
                     RLNLHEADFAWLTHKIIQTASGCPGKIVSVLEGGYTLEPLAQSAAEHIRVLAGLGKSD
                     AATAYQKTLDPTKKRFAKPKTGQVRQPTQSDRY"
BASE COUNT         2507 a         2600 c         2427 g         2260 t
ORIGIN      
        1 ctgcgacgct taacggctgt ctttccactg ataatatttc gagccgagga aagaaccgag
       61 ctttctcaaa aacggttgga ggatgatatt cggctggcgc aactgctcaa acggcttcag
      121 acggcattcg tcccctaaaa ttgcttcggc aaccgccaga cctgcaatgc ctgttatcgc
      181 catcccgtgt ccggaataac cttgcgtata aaaaacattc ggggccaaac gtccgaaatg
      241 cgggacaagg ttggcggtaa tatcgcactc cccgccccac gaatattcga ttttgacatc
      301 ggcaagctgc ggaaaaactt taagcatatc ttggcggaca agctcggtca tacgcgcagg
      361 attgtcgata aactcgttat ccttaccgcc gaaaagcagt ctgccgtccg cgctgaggcg
      421 gtaataatcc aaaatatggc ggttgtcgca tacagccata ttgttgcgga taagcccttt
      481 tgcgcgcgcc cccaagggtt cggtggcaat aataaaggtg ctaacggcaa tcgccttacg
      541 ctccaaaggc ttgaatatcg gattcaaacc ggcataggta ttgacagcat agaccacatt
      601 tttgcactcg acgctgcctt cgggcgtgta aaccagccaa ccgttttgat gcggttcgat
      661 gcacgtcatc ggggattgct cgaaaatctg cgcaccggct tcggcagcgg cacgagcgat
      721 gcccaaagtg taattgagcg gatgcaggtg tccggataag gggtcgaatt gtgccccttg
      781 gtacatatcg ctgtcaagct gctgtttcaa ctcggcttta tcccaaagtt gataatgact
      841 cgcaccgtaa tgccgttggg cgtgttcatg ccactgctgc aactcttccc aatgctgcgg
      901 acggacggca accgtggcat aaccgcgctg ccaatcgcaa tcaatggcat gtttgcggac
      961 gcgttcgtcc accagttcga ccgcctgcaa agactgttgc caaaaccatt gcgcctgctc
     1021 caagccgacc tgtttttcaa tttcccccat accgcaggcg taatcgctga taacctgccc
     1081 gccactccgt cccgacgcgc cgaaaccgat acgcgcggct tccaacacaa ccgtttcatg
     1141 tccctgctcc gccaagggca atgcagtgca caaaccactc aatccgccgc cgatgataca
     1201 ggtatcggtt ttcagacggc attgaagttt cggataaaca gtatgaggat taaccgaact
     1261 aaaataataa gaaggcagat attcttgaaa atcaggacga atcattatgt ttactttatc
     1321 aggtgtattt tcagacggca tgatatggtt tgccgtacca tgccgtctga acgggaaatt
     1381 actttaagaa aacaggctga cctgataatg cggtcagcct gccctgtcgg tcaatgcgct
     1441 ttctcagcag ccagtttttc ctggcggtag gcttctgccg cttctcggtc acgcctggtc
     1501 gcctgcctca tcatccaata attgacgatg atgaccaatg ttccgatgat gccgatcagg
     1561 atggttgcca aaacgttcat ctgcggatca agacccagct tgattttgga gaaaatcacc
     1621 tgcggcaatg tggatgaacc ggggccggag aggaatgagg taattaccaa atcatccaaa
     1681 gacagggtaa tgccgagcag aaagcctgaa gcgatggcag gggcaatcaa aggcaaagtg
     1741 atgacaaaaa agattttcag cggacgcgcg cccaaatcca ttgcggcttc ttcgagcgat
     1801 tggtcaagct caaccagacg cgaacggata acaacggtaa tgtacgccat acacagcgtc
     1861 gtatgtccga ggaagatggt gaaaaagcca cgatcgaagt agagatgttg taaccattcg
     1921 ctgccctgca aaaatatctg tacctgaata atcagcagca gcatagacag accggtaatc
     1981 acgtcgggca tcaccatagg tgcggaaatc ataccggcga acaaggtact gccgcgaaaa
     2041 cgtttaatcc gcgccatcgc atagcctgcc agcgtgccca aaacgacggc agcaagcgaa
     2101 gacacaacgg caatccgcag cgacagccaa gcggcttcca agatggtgtc gttttccagc
     2161 aatgcgccgt accacttggt cgaaaagccg ccccaaacgg ttaccagctt ggattcgtta
     2221 aacgaataga tgaccaaaac aaccagcggg atatacagaa acgccagcga cagtgccaac
     2281 atcagtttca agaaccaaga taatttggat ttctgcatta tttggctcct tcttccaatt
     2341 cgcggttttc ataatgctga aacagggcaa tcggcacgac cagcagcgcg accatcacga
     2401 cggcgacggc ggaagccagc ggccagttgt tttgatcgaa gaacgcctgc cacaagactt
     2461 taccaatcat caggttttcc gaaccgccga ccagctcggg aatgacgaac tcgccgacag
     2521 cagggacgaa aaccagcatg gagcctgcaa taatgccggt tttcgacaaa ggcagggtaa
     2581 tcgtcaagaa cgatttgacc ggccccgcgc ccaaatcgga agccgcttca agcaggcggt
     2641 tgtcgagttt caccagttgc gtgtatagcg gcagaatcat aaacggcaga taggcgtaaa
     2701 ccatcaccaa attgagcgaa aaggcattgt agaacaaatc caaaggctcg ctgataatac
     2761 ccattttaat caacaggttg tttacaatac cgttatgccc gagcagaccc atccacgcat
     2821 agacgcgcaa caggaacgat gtccaaaagg gcagcataat ggcaagcagc aaaccattgc
     2881 ggacagaagg attggcacga gaaatcgcat aggcggtcgg ataaccgacc aacaggcaaa
     2941 tcactgtcgt catcaatgcg gttttgattg aagaccagta ggtcatcaga tagatattgc
     3001 tgttttcact gtcgccgaac gggttaaggg tatgccaaaa attttggaaa atatcggcat
     3061 agttttgata actgatggtg atattcagac gacctaaatc ttcgtcgatg ctggtcagcg
     3121 gcgtaaacgg cggaatggcg atttcttgtt cggcaaagct gattttcagc acgatggcga
     3181 acggaatcag aaacagcacc aaaagccaaa tatacggtac ggcaatcacc acacgctgcc
     3241 ccgggcggcg gaacagtttg tttttcagtt tattaaggtt cattgcattc cccttaaatc
     3301 aacggaacag tggcgtaggc tggttttccg gccagctgat atacacggtt tcgtcccaag
     3361 ttggcggtgt aatgttgcgt acataccagt aaggtgcagg aacttggctt ttgacgacgc
     3421 gcccgttggc gagtttgata tggtatatgg caaagctgcc caaataggcg atttctttta
     3481 ccgtgccttt cgcccagttg tagtcgccca aatattcggg tttttcttta tataaatcaa
     3541 tatcctctgg tcgaatacta acccaaaggt cctgctcgct cggaccaccc aaaccgtgat
     3601 cgatgcggac gtggttttcc aaaccttcgc attcgataac ggcatagtcg gcatgatctt
     3661 caatcaccac accgtcaaag atgttggttt cgccgataaa ctcggcagtg aagcggctgt
     3721 tgggatagtc gtacacgtcg ctgggtgtgc cgacttgctg caactgaccg tcagacataa
     3781 tggcgatgcg ggtcgccatc gtcatcgcct cttcttggtc gtgcgtaacc ataatacagg
     3841 ttacgccgac ttgttccagc gtattgacca actcaagctg ggtttgttgg cgcagttttt
     3901 tgtccaatgc accgaggggc tcatccagca gtagaatttt cggacgtttt gccagactgc
     3961 gtgccaaagc aatgcgctgc tgctgaccgc cggacaattg gtgcggtttg cgtttagcaa
     4021 atttggtcat ctgaaccagg cggagcattt cttcgacgcg cgcggcgatt tcgcctttag
     4081 gcattttgtc ctgtttcaga ccgaaggcaa tgttttgttc tacggtcata tgcggaaaaa
     4141 gcgcgtaact ttggaacatc atattgatgg ggcgatcata gggtgcaagt ttggtaatat
     4201 cctgaccatc aaggataatt tttccctgat tgggactttc catacccgcc agcatacgca
     4261 gcagtgtaga ttttccgctg ccggaactgc ccaaaagggc gaagatttcg tgttgataaa
     4321 tgtccaagtc gatgttatcg acagcgtaat tgtcaccaaa ctttttcacc aaaccttgga
     4381 ttttgagata aggtttggct gaagacgcag tggttgcggt cataatggca atactccaat
     4441 aaaaagacga gtaccggcaa aacggatttt cgaatgggtg ataaaaagct gtttgattgc
     4501 tggcgggagt taaacgtttg atgccgtctg aaactcttgt aaagcgcacg ggcagcatga
     4561 aatggaacaa gattccaaag aactttatat tatattagtt tatgcggttt tcgggcaata
     4621 tagtggatta aatttaaacc agtacagcgt tgccttgcct tgccgtacta tttgtactgt
     4681 ctgcggcttc gtcgccttgt cctgatttaa atttaatcca ctataaatat ggatttgagc
     4741 ttgtcggaaa gcaacagaaa agaaaacacc gcccattttt ctgggcggtg tcggaaagcg
     4801 taattattta cgcagaccca agcgggtaat caacgcgcga tacgtatcgg gctgggtacg
     4861 gcgcaagtag gccagcaggc ggcggcgttg gctgaccatt ttcaacaggc cgcgacggct
     4921 gtggtggtct ttggggttgg ctttgaagtg gggggtcagg tcgttgatgc ggaaagtcaa
     4981 cagagcgact tgtacttcgg aagagccggt gtcgccttct ttgcgttgga aatctttaac
     5041 gatttgtgct ttttgttcta cggtcagtgc cataatgaaa actccaaaaa tataagaatc
     5101 cccataggga ttcagacaag tttgccaagc ctgaagacaa cggcaaactc cctatgccca
     5161 agataggaca acgtggcatt atgacacaat tcccgcattt ctgcacaata ttttaagacc
     5221 gtggaagtaa ccgttaaaaa tgccgtctga agcattgtct gcttgagacg gcaatgttca
     5281 gacggcatat gcgcttcaaa ccacgatttc ttctttcttc ttcggttctt taccgatatt
     5341 gtcgcggctc aaaccgaaca ttagcagaag cgggctggca accaatacgg aagaataaat
     5401 gccgaacacg atgccaatgg tcaacgccat agaaaagccg tgcaaggccg caccgccgaa
     5461 caccagcata gaaacaacca tcgcctcggt cgaaccgtgg gtaatgatgg tgcggctcat
     5521 cgttgcggta atcgcgttgt cgatgacttc cggcacggca tgtccgcgca tcgccggctt
     5581 gcggaagttt tcacggatac ggtcgaagac gacgacggat tcgttcacag aatagcccaa
     5641 tacggcaagg atacccgcca agacggtcag cgaaaattcc cattggaaga aggcaaagca
     5701 gccgagaata atcacgatgt cgtgcatatt ggcgataatg gcagatacgg caaaacgcca
     5761 ttcaaaacgc atcgacaggt aaataatgat gccgataacg acaaaaccta aagccatcaa
     5821 tccattactt accaattcct caccgacttg cgggccgata aattcgactt ggcgcaaggt
     5881 aacgtcggga ctgtcttttt tcagcaaatc cataacctga ttggacaact gtgcggaagt
     5941 aacaccttct ttgttcggca ggcggatcat gatgtgtttg ttcgtaccca atgcctgaac
     6001 ctgtacatca cctattttca gcgtatcgag gcgttcgcgc atcttattga catccgcacc
     6061 ctgctgatat tggacttcca ttaccgtacc gccggtaaat tcgacagaga aattcaggcc
     6121 tttggtcacc aagaagaaca cggcagcgat aaacgtaacc aacgaaatga aggtcgtcag
     6181 tttgccgtag ctcataaacg gaatatcgcg tttgatttta aagagttcca tagcttactc
     6241 cttgcctcct gccatttcgg ctttcggctt ccacaccgaa ccgatggaaa tattctgcaa
     6301 tttgcgtctg cgtccatacc acagattgac caacgcacgg aatacgacga cggacgaata
     6361 catcgaagtc agaataccca aacagtgtac gaccgcaaaa ccgcgtaccg ggccggaacc
     6421 gaataccaaa agcgcgatac cggcaatcag cgaagtcagg ttggaatcga caatggtcgc
     6481 ccatgcgtgt tggaaaccga gattgattgc ctgctgcggc ggcacgccgg cacgcaattc
     6541 ttcgcggata cgttcgttaa tcaagacgtt ggagtcgatt gccataccca aagtcaacgc
     6601 cagcgcggcc atacccggta acgtcaacgt tgcctgcatg gcagacaaaa taccgattag
     6661 gaacagtatg ttggcactca atgcaatggt agaaaagaaa cccatcagac gatagtaaac
     6721 caccatgaat gcagcaacga tggcaaaacc ccataaagtc gaatggaagc ctttttcgat
     6781 gttctcctta cccaaagacg gaccgatggt acgttcttcg acaatctgca tcggcgcggc
     6841 aagagaaccg gcacgcaaca gcaaagacgt atcattggct tcggctgtcg tcatgcttcc
     6901 ggaaatttcc acgcgtccgc cggtaatggc agtacggata accggcgcgg ttacaacctc
     6961 ggattttcct tggtcgatca aaaccatcgc catgcgtttg ccgacatttg cggcagtcag
     7021 ttcgccaaaa atgctgccac ccgcgctgtc caagctcaga ctgacggcag gtgcgcccat
     7081 ttggtcgaaa ctcggttgcg catcgttgat gttgtcgccc gtcagctcga cctgtttgct
     7141 gattagcaga gtttcgggac gatctccgcc gcttgaaagc agctcataac cgctcggcac
     7201 gttgccttcc aatgcctcgc gcaacttggc aggatcgtcc tccaccatac gcaattccaa
     7261 agtcgcggta cggccgatga tgtcttttgc cttggcagta tcctgaacgc ccggaagctg
     7321 cacgacgata cggtcggcac cggactgctg gatgacgggt tcggccacgc ccaactcgtt
     7381 cacacggttg tgcagggtag tgatgttctg tttgaccgca tcggaacgca ctttattgac
     7441 cgcctcttcc gaaagcgtca agacgatatt gctgccgtct gaattcagcg ttgcttcagg
     7501 aaacagcttg cgcaactgcg gcagagcctt ttgcacatca cctgcatcct gcaaagggac
     7561 ggtcaggctg tttccagcct gacgcaccgt gccgctgcgg attttttcgc ggcgcagttc
     7621 gcggcggatg tcgcccgaat aacgttcaaa cgttttctgc atcgccgctt tcatatcgac
     7681 ctgcatggtg aaatgcacgc cgccgcgcaa gtccaaaccc aaaaacatcg gattggcttt
     7741 gattttcgcc atccattcgg ggctgtccgc caacaggttg agcgcggtaa tatacccttc
     7801 gcccaaagtg ttttcgatga cgtcgcgcgc tttaagctgc gtttctgtgt ctttgaaacg
     7861 cactttcagt gaattgtcca caacaaacat cccgtcggtc tgaatacctg cgtttttcag
     7921 cgcggcatcc actttgaatt gagtctgttc gttgatgatg atggcttgtc ggttggtcga
     7981 tacctgcacg gcgggtgttt cgccgaatag gttgggcagc gaatacactg cggcaaccgc
     8041 aatcgtgaac acaatcagca gatatttcca taaaggataa cggttcatca ttgttcctta
     8101 atggttggaa ccccaccctt tcggtggtgt cggaatcggg ctatttcaga agaggcaaaa
     8161 acccttccca gccaggcaag accggaaagc ggcatcctga atatgccgcc ctgcgtgtcg
     8221 gaacatggtc aagccttcgg ttggaattca aaacaaagtg ccgcattcgg gcttttccga
     8281 tgcggcttgt cggcacaaat caatcgactt ttgcagaaat cgcattgcgt tccacttcga
     8341 cctcgatttt tgtactctgt ccgatatcca cggtaaaaaa ctgttcgccg actctggtta
     8401 ccttaccctt gaaacctgcc gccaagacca ctttgtcgcc gactttcaag gcggcaagca
     8461 ttgcctgatg cgctttgaat ttcttttgct gcggacgcat gatcaggaag tagaacacca
     8521 ccataatcaa cactaaagga gcaaattgtg caacagcttg attcataatt tatccgttct
     8581 ttctaatatg gttgaaaatc gagaggggta tataataaca taagaccgta aacaatatat
     8641 cgggtttgcc gtccgtaccg accgtatacc gcagcctgcc cgtccacaaa cccatgtcct
     8701 tgacccgcct aatcttgaaa ttctatgcac tgttgcgcct ttttttgggc aaaaacgccc
     8761 gcaccgcatg gatttcgcat cccgcctgtg ccgggcacga acccggcgca aaccatcccg
     8821 attcgcccga ccgcatcctc tgcatcgagc aggcattgcg ccgcgccggt atttggcagc
     8881 acctccaaac catagaggcg gaagaaatca gcgatacgcg cctcgcactt gtccactcga
     8941 gcaaatatct gaaccgtttg gaatcttgcc agccccaaaa aggcaagatt ttccgcctgg
     9001 atgacgacac tgcaatcagc acaggctcgc tgtctgccgc acgctttgcc gccggttcgg
     9061 cagttcaggc agtcgacatg gtcatgaacc gcaaagcatg gcatgccttt tgcgccgccc
     9121 gcccgcccgg acaccatgcg ggcagcggca aagccggcgg attctgcctg ctgaacaacg
     9181 ttgccgccgg cgtcatgcat gccattgccg aataccgcct gaaacgcatt gccgtcatcg
     9241 atttcgatgt ccactacggc gacggtacgg cagaaatatt caaagacgat ccgcgcatcc
     9301 tgtttttcaa cctgtttgaa accgaccttt tccccttccc cgaaaacaac gatatgcccg
     9361 acggcggcaa tatggtgcac ctgcccttgc cgccgggaac gggcagccgc acattccgcg
     9421 aagccgtccg caggcagtgg ctgccccgac ttgccgcatt caaacccgaa ctggtgctgc
     9481 tgtcggcagg attcgacgca caccgtctag acgaatcggg caggctcaac ctgcacgagg
     9541 cggattttgc ctggctgaca cacaaaatca ttcagacggc atcgggctgc cccggcaaaa
     9601 tcgtatccgt actcgaaggc ggctacaccc ttgaacctct ggcacaatct gctgccgagc
     9661 atatccgcgt gctggcaggg ctgggcaaat ccgatgccgc aaccgcctat cagaaaacac
     9721 tggacccaac aaaaaaacgg tttgccaaac cgaaaaccgg acaggtgcgc caacctaccc
     9781 aatccgaccg ttac
//
LOCUS       DAWHSK010000045         9731 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.045, whole genome shotgun sequence.
ACCESSION   DAWHSK010000045 DAWHSK010000000
VERSION     DAWHSK010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9731
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.045"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(128..1174)
                     /gene="adhP"
                     /locus_tag="WIC03_000755"
     CDS             complement(128..1174)
                     /gene="adhP"
                     /locus_tag="WIC03_000755"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEZ3883287.1"
                     /translation="MKMQAVVVNKNVAGDVEIVEREVRPLEYGEALVEVEYCGVCHTD
                     LHVAAGDYGEKPGRVLGHEGIGLVKEVADGVKNLKVGDRVSIAWLFQSCGSCEYCNTG
                     RETLCRSVLNAGYTADGGMATYCIVSADYAVKVPEGLDPAQASSITCAGVTTYKAIKV
                     SSVRPGQWIAIYGAGGLGNLGVQYAKKVFGAHVVAIDINDDKLAFAKETGADLVVNAA
                     KEDAAKVIQEKTGGAHAAVVTAVSAAAFNSAVNCVRAGGRVVAIGLPPESMDLSIPRL
                     VLDGIEVVGSLVGTRKDLEEAFQFGAEGLVVPKVQLRALDEAPAIFQEMREGKITGRM
                     VIDMKKECGCGHHH"
     gene            complement(1457..1846)
                     /locus_tag="WIC03_000756"
     CDS             complement(1457..1846)
                     /locus_tag="WIC03_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HEZ3883288.1"
                     /translation="MKNVQKGFTLLELMIAVAILGILTLITYPSYKTYIRRVRLSEVR
                     TTLLHNAQTMERYYRQKGTFKTYDQTKLKQNKYFNVTLSKVSPDHFTLQADPNPTTND
                     GETCVVTLNDGGTIAASGTNQSCPGFD"
     gene            2072..3250
                     /locus_tag="WIC03_000757"
     CDS             2072..3250
                     /locus_tag="WIC03_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacA family efflux pump subunit"
                     /protein_id="HEZ3883289.1"
                     /translation="MAKMMKWAAVAAVAAAAVWGGWSYLKPEPQAAYITEAVRRGDIS
                     RTVSATGEISPSNLVSVGAQASGQIKKLYVKLGQQVKKGDLIAEINSTSQTNTLNTEK
                     SKLETYQAKLVSAQIALGSAEKKYKRQAALWKENATSKEDLESAQDALAAAKANVAEL
                     KALIRQSKISINTAESELGYTRITATMDGTVVAILVEEGQTVNAAQSTPTIVQLANLD
                     MMLNKMQIAEGDITKVKAGQDISFTILSEPDTPIKAKLDSVDPGLTTMSSGGYNSSTD
                     TASNAVYYYARSFVPNPDGKLATGMTTQNTVEIDGVKNVLIIPSLTVKNRGGKAFVRV
                     LGADGKAAEREIRTGMRDSMNTEVKSGLKEGDKVVISEITAAEQQESGERVLGGPPRR
                     "
     gene            3316..5250
                     /locus_tag="WIC03_000758"
     CDS             3316..5250
                     /locus_tag="WIC03_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacB family efflux pump subunit"
                     /protein_id="HEZ3883290.1"
                     /translation="MSLIECKNINRYFGSGENRVHILKDISLSIEKGDFVAIIGQSGS
                     GKSTLMNILGCLDTAGSGSYRIDDIETAKMQPDELAALRRERFGFIFQRYNLLSSLTA
                     RDNVALPAVYMGAGGKERSARAEKLLQDLGLASKEGNKPGELSGGQQQRVSIARALMN
                     GGEIIFADEPTGALDTASGKNVMEIIHKLHKAGHTVIMVTHDPGIAANANRVIEIRDG
                     EIISDTSKNPEIPASNVGRIREKASWSFYYDQFVEAFRMSVQAVLAHKMRSLLTMLGI
                     IIGIASVVSVVALGNGSQKKILEDISSIGTNTISIFPGRGFGDRRSGRIKTLTIDDAK
                     IIAKQSYVASATPMTSSGGTLTYRNTDLTASLYGVGEQYFDVRGLKLETGRLFDENDV
                     KEDAQVVVIDQNVKDKLFADSDPLGKTILFRKRPLTVIGVMKKDENAFGNSDVLMLWS
                     PYTTVMHQITGESHTNSITVKIKDNANTQVAEKGLTDLLKARHGTEDFFMNNSDSIRQ
                     IVESTTGTMKLLISSIALISLVVGGIGVMNIMLVSVTERTKEIGIRMAIGARRGNILQ
                     QFLIEAVLICVIGGLVGVGLSAAVSLVFNHFVTDFPMDISAMSVIGAVACSTGIGIAF
                     GFMPANKAAKLNPIDALAQD"
     gene            complement(5568..6359)
                     /locus_tag="WIC03_000759"
     CDS             complement(5568..6359)
                     /locus_tag="WIC03_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HEZ3883291.1"
                     /translation="MKTKLIKILTPFAVLPLLACGQPAVSNANAAPAPAAKAEAPADK
                     SVAASLKARLEKIYSAQDLKVLSVSETPVKGIYEVVVSGRQIIYTDAEGGYMFVGELI
                     NIDTRKNLTEERAADLNKIDFASLPLDKAIKEVRGNGKLKVAVFSDPDCPFCKRLEHE
                     FEKITDVTVYSFMMPIAGLHPDAARKAQILWCQPDRAKAWTDWMRKGKFPVSGSICDN
                     PVAETTSLGEQFGFNGTPTLVFPNGRSQSGYSPMPQLEEIIRKNQ"
     gene            6495..8684
                     /locus_tag="WIC03_000760"
     CDS             6495..8684
                     /locus_tag="WIC03_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEZ3883292.1"
                     /translation="MFYHRIAVNVPLSDGLLTYSYSEPLPPGTRVLVPFRNKTVVGIV
                     WETDISPDMDTARILSVQTAFSDEPPLPQSWRDLLAFTSRYYHYPTGQAVFAALPQGL
                     KETRAVEMPQPPLFYALNEAGRAQTPPPARFNKKAALWDALLSGEMTMAALKQVNAQA
                     AKLIENWAEQGWIETTEAAKPVLRSCHGQASHSEFVLNADQRQASDAIQTALGRFRPF
                     LLYGITGSGKTEVYFDAMAKVLAQGRQVLFLLPEINLTPQLLKRVENRFADVPTAVLH
                     SQMAAGRRTQDYLRAMLGQAKLVIGTRLAVFTPMDDVGLIVVDEEHDGSFKQDNELRY
                     HARDLAVWRAKQSGCPVVLGSATPSLESWHKVQSGAYRLLQLTERAHTAAQLPQVDIL
                     NVGRLKLDNGFSPQALQLLKQNFEAGGMSLVYLNRRGFAPALFCGDCGHTFGCPNCSA
                     KMVLHQRVRQLRCHHCDHREPIPFKCPDCGNQDLTAVGHGTQRVEETLRAFLPKAAVV
                     RVDRDSTAHKNDWADLYRRIADNEIDILVGTQMLAKGHDFARLNLVIVLNADGSLYSA
                     DFRAPERLFAELMQVSGRAGRADKPGKVLIQTQLPEHPVFAAVKAQDYAVFAENELNE
                     RQMFAMPPFGFQTAVRADAPRVADALEFLNAAKETLAPLLPASVSQFGAAPMLMVRLA
                     ERERAQIFLESPSRQDLHRAVSLWVQVLQQNRDGKIRWSVDVDPQEA"
     gene            complement(8731..9625)
                     /locus_tag="WIC03_000761"
                     /pseudo
     CDS             complement(8731..9625)
                     /locus_tag="WIC03_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703681.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
BASE COUNT         2252 a         2515 c         2839 g         2125 t
ORIGIN      
        1 gacgtgttgc gatacgggtt ttcagacggt atttcaaaca gaacagatta aaatcaaatc
       61 caaatccata aaaaatgccg tctgaacagc gttcagacgg catttcgatg tgtactgcca
      121 cgtcaaatca gtggtgatgg ccgcagccgc attctttttt catatcgatt accatacggc
      181 cggtgatttt gccttcgcgc atttcttgga aaatggcggg tgcttcatcc aaagcacgca
      241 gttggacttt cggcacaacc aaaccttccg cgccgaattg gaaggcttct tccaaatctt
      301 tgcgcgtgcc gaccaaagag ccgaccactt cgatgccgtc caaaaccaaa cgcgggatgg
      361 acaaatccat cgattccggc ggcagtccga tggcaaccac acgtccgccc gcgcggacgc
      421 aattcacggc agagttgaat gcggcagcag atacggcggt tacgaccgca gcgtgtgcgc
      481 cgcctgtttt ttcctgaatc actttggcag cgtcttcttt ggcggcgttg acaaccaaat
      541 ccgcgccggt ttctttggca aacgccagtt tgtcgtcgtt gatgtcgatg gcgacaacgt
      601 gtgcgccaaa tacttttttc gcgtattgga cccccaagtt gcccaaaccg cccgcgccgt
      661 agatggcaat ccactgtccc ggacgaacac tggaaacttt aatggcttta taagtggtca
      721 cgccggcaca agtaatgctg gaagcttgcg caggatccaa accttcaggg actttgaccg
      781 cgtaatcggc actcacgatg cagtaggtcg ccataccgcc gtcggcggtg tagcccgcgt
      841 tcaatacaga gcggcacaag gtttcgcggc cggtattgca gtattcgcaa gagccgcagc
      901 tttggaacag ccaggcgatg ctgacgcggt cgccgacttt cagatttttc acaccgtcgg
      961 caacttcttt aaccaaaccg atgccctcgt gtcccagcac gcggcccggt ttttcgccgt
     1021 agtcgcctgc cgcaacgtgc aggtcggtgt ggcacacgcc gcaatattcg acttcgacca
     1081 acgcctcgcc gtattccaac gggcgaacct cgcgttcgac tatttccaca tcgcccgcta
     1141 catttttatt cacaacaact gcctgcattt tcatgattgc gctccttagt tacggcaaaa
     1201 aaacctgtaa acggaatgtt gtccgatata agggtaagca tacgcttccg catctcacag
     1261 gtcaagtggt atgttgttga aaaatataga ttatatgtta tattataaca gcttggaaag
     1321 gcgcggcatc ggggcggttg ccggatgagg ggcggcaggt ttcaagtttg aaaaaccgga
     1381 cggcaaaccc gtaaagatac cgtctgaagc tgtgtccgga cggcatcttt acgggtttgc
     1441 gggcttcggc ggaggattag tcgaagccgg ggcaggattg gtttgtaccg gaagcggcaa
     1501 tggtaccgcc gtcgttgagc gtaacgacgc aggtttcgcc gtcgttggtg gtgggattgg
     1561 ggtcggcctg aagggtaaag tggtcggggc tgactttgct taaggtaacg ttgaaatatt
     1621 tgttttgttt cagtttggtt tggtcgtagg ttttaaacgt ccctttttgg cggtagtaac
     1681 gttccatggt ctgcgcgttg tgcagcaggg tcgtcctgac ttccgacagg cggacgcgcc
     1741 ggatgtaggt tttataggaa gggtaggtga tgagcgtcag gatgccgagg atggcgacgg
     1801 caatcatcag ctcgagcagc gtaaagcctt tttgaacgtt tttcatagca atgtgtttcc
     1861 atttgtttgt cggtcggcga cattataacg ccgtatcgga aatggcggaa tatgtaaacg
     1921 gattgaaatt ttcgggaaag cagattgtat aagccattta aaacaaatgg ttatttttat
     1981 tgtcggcagt ttgccgcctt ggatggggca gggacttgcg gtagaatccg cttccgattt
     2041 atgggattga cgcatacaga gaattgaaaa catggcaaaa atgatgaaat gggcggctgt
     2101 tgcggcggtc gcggcggcag cggtttgggg cggatggtct tatctgaagc ccgagccgca
     2161 ggctgcttat attacggaag cggtcaggcg cggcgacatc agccggacgg tttctgcaac
     2221 aggggagatt tcgccgtcca acctggtatc ggtcggcgcg caggcatcgg ggcagattaa
     2281 gaaactttat gtcaaactcg ggcaacaggt taaaaagggc gatttgattg cggaaatcaa
     2341 ttcgacctcg cagaccaata cgctcaatac ggaaaaatcc aaattggaaa cgtatcaggc
     2401 gaagctggtg tcggcacaga ttgcattggg cagcgcggag aagaaatata agcgtcaggc
     2461 ggcgttatgg aaggaaaacg cgacttccaa agaggatttg gaaagcgcgc aggatgcgct
     2521 tgccgccgcc aaagccaatg ttgccgagct gaaggctcta atcagacaga gcaaaatttc
     2581 catcaatacc gccgagtcgg aattgggcta cacgcgcatt accgcgacga tggacggcac
     2641 ggtggtggcg attctcgtgg aagaggggca gactgtgaac gcggcgcagt ctacgccgac
     2701 gattgtccaa ttggcgaatc tggatatgat gttgaacaaa atgcagattg ccgagggcga
     2761 tattaccaag gtgaaggcgg ggcaggatat ttcgtttacg attttgtccg aaccggatac
     2821 gccgattaag gcgaagctcg acagcgtcga ccccgggctg accacgatgt cgtcgggcgg
     2881 ctacaacagc agtacggata cggcttccaa tgcggtctac tattatgccc gttcgtttgt
     2941 gccgaatccg gacggcaaac tcgccacggg gatgacgacg cagaatacgg ttgaaatcga
     3001 cggcgtgaaa aatgtgctga ttattccgtc gctgaccgtg aaaaatcgcg gcggcaaggc
     3061 gtttgtgcgc gtgttgggtg cggacggcaa ggcggcggaa cgcgaaatcc ggaccggtat
     3121 gagagacagt atgaataccg aagtaaaaag cgggttgaaa gagggggaca aagtggtcat
     3181 ctccgaaata accgccgccg agcagcagga aagcggcgaa cgcgtcctag gcggcccgcc
     3241 gcgccgataa acgaatatgc cgtctgaaca cggaaacggt ttcagacggc atttgttatt
     3301 gatttacgga atattatgag cttgatcgaa tgtaaaaaca tcaaccgcta tttcggcagc
     3361 ggcgagaacc gcgtccatat tttgaaagac atcagcctgt cgatagagaa gggcgatttt
     3421 gtcgccatca tcgggcagtc cggttcgggc aagtccacgc tgatgaacat actcggctgt
     3481 ttggataccg ccggttccgg ttcgtaccga atcgacgaca tcgaaactgc caaaatgcag
     3541 cctgacgagc tggcggcatt gcgccgcgaa cgcttcggtt tcatcttcca acgctacaac
     3601 ctcttaagct cgctgaccgc aagggacaac gtcgcgctgc cagccgtcta tatgggcgcg
     3661 ggcggcaaag agcgttccgc gcgtgcggag aagttgttgc aggatttggg tttggcaagc
     3721 aaagagggca acaagcccgg cgaactctcg ggcggacagc agcagcgcgt ctccatcgcc
     3781 cgcgccctga tgaacggcgg agaaatcatc ttcgccgacg agccgaccgg tgcgctcgat
     3841 accgccagcg gcaaaaacgt gatggaaatc atccacaagc tgcacaaagc cgggcatacc
     3901 gtcattatgg tcacgcacga ccccggcatc gccgccaatg ccaaccgcgt catcgaaatc
     3961 cgggacggcg aaatcatttc cgacacctcg aaaaatcccg aaatccccgc aagcaatgtc
     4021 gggaggattc gggaaaaagc ctcgtggtcg ttttattacg accagtttgt cgaagccttc
     4081 agaatgtcgg tgcaagcagt attggcgcac aaaatgcgtt cgcttctgac gatgctcggc
     4141 atcatcatcg gcatcgcgtc ggtggtttcc gtcgtcgcat tgggcaatgg ttcgcagaaa
     4201 aaaatccttg aagacatcag ttcgataggg acgaacacca tcagcatctt cccggggcgc
     4261 ggcttcggcg acaggcgcag cggcaggatt aaaaccctga ccatagacga cgcaaaaatc
     4321 atcgccaaac aaagctacgt tgcttccgcc acgcccatga cttcgagcgg cggcacgctg
     4381 acttaccgca ataccgacct gaccgcttct ttgtatggcg tgggcgaaca atatttcgac
     4441 gtgcgcggac tgaagctgga aacggggcgg ctgtttgacg aaaacgatgt gaaagaagac
     4501 gcgcaggtcg tcgtcatcga ccaaaatgtc aaagacaaac tctttgcgga ctcggatccg
     4561 ttgggtaaaa ccattttgtt caggaaacgc cccttgaccg tcatcggcgt gatgaaaaaa
     4621 gacgaaaacg ctttcggcaa ttccgacgtg ctgatgcttt ggtcgcccta tacgacggtg
     4681 atgcaccaaa tcacaggcga gagccacacc aactccatca ccgtcaaaat caaagacaat
     4741 gccaataccc aggttgccga aaaagggctg accgatctgc tcaaagcgcg gcacggcacg
     4801 gaagatttct tcatgaacaa cagcgacagc atcaggcaga tagtcgaaag caccaccggt
     4861 acgatgaagc tgctgatttc ctccatcgcc ctgatttcat tggtagtcgg cggcatcggc
     4921 gtgatgaaca tcatgctggt gtccgttacc gagcgcacca aagaaatcgg catacggatg
     4981 gcaatcggcg cgcggcgcgg caatattttg cagcagtttt tgattgaggc ggtgttaatc
     5041 tgcgtcatcg gcggtttggt cggcgtgggt ttgtccgccg ccgtcagcct cgtgttcaat
     5101 cattttgtaa ccgacttccc gatggacatt tccgccatgt ccgtcatcgg cgcggtcgcc
     5161 tgttcgaccg gaatcggcat cgcgttcggc tttatgcctg ccaataaagc agccaaactc
     5221 aatccgatag acgcattggc acaggattga ggttggacaa agatgccgtc tgaagctgcc
     5281 ggaccggtca ttttggagca gaaacttatt ggataaaaac ggtttcttag attctacgtt
     5341 ccagattccc acttgcgtgg gaatgacggc ggtgtgtttc gtattgttca gtttattatt
     5401 ttcaatcatt caatgggtta ggatgtgttt gttggcttgc tgtactggtt taaatttaat
     5461 ccactataaa tttaatccac tataactttc agtgcggatt ggttttcagg cggcatttcc
     5521 cgcaaaaaag gcttggaatt ttccaagcct tttgttttgc ggacgggtta ttggtttttg
     5581 cggatgattt cctccagttg gggcatcggg ctgtagccgc tttggctgcg cccgttgggg
     5641 aagacgaggg tcggcgtgcc gttgaagccg aattgttcgc ccaaggaagt ggtttccgcg
     5701 acgggattgt cgcagatgct gccgctgacc gggaatttgc ctttacgcat ccaatccgtc
     5761 cacgctttgg cgcggtcggg ctgacaccat aagatttgcg ccttgcgcgc ggcatcaggg
     5821 tgcaggccgg caatgggcat cataaagctg taaaccgtca cgtcggttat tttttcaaac
     5881 tcgtgttcca agcgtttgca gaacggacaa tcggggtcgg aaaagacggc gactttcagc
     5941 ttgccgttgc cgcgcacttc tttgatggct ttgtccaaag gcagggaggc gaagtcgatt
     6001 ttgttcaaat cggcggcgcg ttcttcggtc aggtttttgc gcgtgtcgat gttgatgagt
     6061 tcgccgacga acatatagcc gccttcggca tcggtgtaga taatctgcct gccgctgacg
     6121 acgacttcgt aaatgccttt gaccggtgtt tcgctgacgc tcaacacttt caaatcttgg
     6181 gcggaataga ttttttccaa acgcgctttc aaagaagcgg caacggattt gtcggcaggc
     6241 gcttccgctt ttgcggccgg agcaggcgcc gcgttggcgt tggatacggc aggttgaccg
     6301 catgccaaca aaggaaggac ggcaaacggt gttaagattt tgattaactt ggttttcatg
     6361 cgagagactt gcgtgtgttg aaagtaaggc attgtatcaa acctctgtca tgtctgcatt
     6421 gtactcaggc tcaaattatc gtctgaaaat agctttcggc tgttaaaata cgaaaaaata
     6481 atttgattgt ttgtatgttc tatcaccgta ttgccgtaaa cgtgccgctt tcagacggcc
     6541 ttttgacgta ttcctattcc gagccgcttc ctccgggaac tcgggtgctt gtgccttttc
     6601 gcaataaaac cgttgtcggg attgtgtggg aaacggatat ttcgcccgat atggatacgg
     6661 cgcggatttt gagcgttcag acggcctttt cggacgaacc gccgttgcct caaagctggc
     6721 gtgatttgtt ggcatttacg tcgcgttatt accactatcc gactgggcag gcggtgtttg
     6781 ccgctctgcc acagggtttg aaggaaacgc gcgcggtgga aatgccgcag ccgccgttgt
     6841 tttatgcgtt gaacgaagca ggcagggcgc aaacgccgcc gccggcgcgg ttcaacaaaa
     6901 aagcggcttt gtgggacgcg ctgctgtcgg gcgaaatgac gatggcagcg ttgaagcagg
     6961 taaacgcgca ggcggcgaaa ttgatcgaaa attgggcgga acagggttgg attgaaacga
     7021 cggaagcggc gaaacccgtt ttaaggtcgt gccacgggca ggcttcgcac tctgaatttg
     7081 tgttgaatgc cgaccaacgg caggcttccg atgccattca gacggcattg ggacgcttcc
     7141 ggccgtttct gctgtacggc atcaccggca gcggcaagac cgaggtgtat ttcgatgcga
     7201 tggcgaaagt gttggcgcag gggcggcagg tgttgtttct gttgcccgaa atcaacctca
     7261 cgccgcagct tttgaagcgg gtagaaaacc gttttgccga cgtgccgacc gccgtgttgc
     7321 acagccagat ggcggcaggc aggcgcacgc aagattattt gcgcgcgatg ttggggcagg
     7381 cgaaattagt catcggcacg cggctggcgg tgttcacgcc gatggatgat gtcgggctga
     7441 ttgtggtcga tgaggaacac gacggctcgt tcaaacaaga caacgaatta cgctaccacg
     7501 cccgcgattt ggcggtgtgg cgggcgaagc agagcggctg ccccgtcgtg ctgggtagcg
     7561 ccacgcccag cttggagagc tggcacaagg tgcaaagcgg cgcgtaccgc ctgctgcaac
     7621 tgaccgagcg cgcccatacc gctgcgcaac tgccgcaagt ggacatcctc aacgtaggcc
     7681 gtctgaaact agacaatggc ttctcgccgc aagccttgca gcttttgaaa cagaattttg
     7741 aagcgggcgg tatgtcgctg gtgtacctca accgtcgcgg cttcgcgccc gcgctgtttt
     7801 gcggcgactg cggccatacc ttcggctgcc cgaactgctc cgccaaaatg gtgctgcacc
     7861 aacgcgtccg ccaactgcgc tgccaccact gcgaccaccg cgaacccata ccgttcaaat
     7921 gccccgactg cggaaatcaa gacctgaccg ccgtcggaca cggcacgcag cgcgtcgaag
     7981 aaaccctgcg cgccttcctg cccaaggcag ccgtcgtccg cgtggacagg gacagcaccg
     8041 cgcacaaaaa cgactgggcg gatttgtacc gccgcatcgc cgacaacgaa atcgacattc
     8101 tggtcggcac gcagatgctc gccaaaggcc atgactttgc gcggctcaac ctcgttatcg
     8161 tattgaacgc cgacggcagc ctttacagcg cggatttccg cgcgccggaa agactgttcg
     8221 ccgagctgat gcaggtgtcc ggcagggcgg ggcgcgccga caaacccggc aaggtgttga
     8281 tacagaccca actgcccgaa catcccgtct tcgccgccgt caaagcgcag gattatgccg
     8341 tgtttgccga aaacgaattg aacgaacggc aaatgttcgc catgccgccc ttcggttttc
     8401 agaccgccgt ccgcgcggac gcgccgcgtg ttgccgatgc gctggaattt ctcaatgccg
     8461 ccaaagaaac cctcgccccg ctgttgcccg caagcgtttc ccagtttggc gccgccccca
     8521 tgctgatggt gcgcctcgcc gaacgcgaac gcgcgcaaat cttcctcgaa tccccgtccc
     8581 gacaggattt gcaccgtgcc gtgagtttgt gggtgcaggt gttgcagcaa aaccgcgacg
     8641 gcaaaattag atggtcggtg gatgtcgatc cgcaggaggc ttgattatcg gcaatacgat
     8701 gccgtctgaa aaccgtttca gacggcattt ttattccgga tcgtctgtaa acgcgttcgc
     8761 ccgaaagatc ggtacgaatg tcagcaggta aagcacgaac acggcggcgg cgaaaaggat
     8821 ggggacggcg atgcggcaga gtttcgggta gtcgagcttg gtaaagccgc tgtgccacag
     8881 tccggcagtc agccacacca tcatcacgcc gcccatcatg ccgccgaggg taatcaggtg
     8941 caggggcgcg gaggcaggca ggttttgcaa tttcgccgcg cctgtccaca aatagcctgc
     9001 ggcggcaaaa agttggagca ggtaataagt gcggacgtag tgtttgcgta agagttcgtg
     9061 atggtgcagt tcgcgcagct tggcgagcaa gatgaagccg acggcgagcg cggtaaaacc
     9121 ggcggtttgc gcgggcagcc aaagttcggc ggcggcgtgc agcagcagga aggtgatggc
     9181 gatgttttta tagacaacgt tggggatgaa tacagggtct ttcagacggc attttttgag
     9241 ggcttccgcg cccagaagga cactgacgcg gacggatacg aacatgaccg ccgccatatt
     9301 caaatgcact tgcgcgcgca acaggttcaa atcgccggtt gcagcgtatg ccgtctgaaa
     9361 aacagtgaac gcggcaagca gcattaagag ggcgaagttg tcggtgtttc ggtctagcca
     9421 aatcagccgg gcgcagaaca gcagcaacac cagccaatag gcggcgacga aaaacgaggc
     9481 agtttgcggc gaaaagggca gtatagcgga tgcggcgagc aataatgccg ccatcaaagt
     9541 cgcgacaggt ttcaggttac ccgaaaaaac ccgtccagtc caacaaagcc gcagtcagga
     9601 aaccaccata tgctgcaggc agcatgagtt ctaagaagat ttggcggtgc aggacgacgg
     9661 caccggggct gatgaaaaac accaacgcgc cgagtatggc aagcactgtc gcgccgacga
     9721 aaaacgggcg c
//
LOCUS       DAWHSK010000046         9686 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.046, whole genome shotgun sequence.
ACCESSION   DAWHSK010000046 DAWHSK010000000
VERSION     DAWHSK010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9686
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.046"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(204..1646)
                     /gene="aldA"
                     /locus_tag="WIC03_000762"
     CDS             complement(204..1646)
                     /gene="aldA"
                     /locus_tag="WIC03_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEZ3883293.1"
                     /translation="MKQLAMYINGRFENDFNGEWRDVLNPSTEETIAREPKGGKADVD
                     RAVAAARAAQPAWERLPAVERGAYLRKIAQGIRGRADELTDTIVAEGGKTKDLARVEV
                     MFTADYLDYQAEWARRYEGEIIQSDRPRENILLFKRPLGVIAGILPWNFPFFLIARKM
                     GPALVTGNTIVVKPSSVTPINCHIFAEIVDAVGLPAGVFNVVNGPGAEIGNALSAHPQ
                     VDMVSLTGSVEAGRQVMEAASANITKVSLELGGKAPAIVLKDADLDLAVKSILASRVG
                     NTGQICNCAERVYVHSSLKDAFIEKMTAAMKGVRYGNPAEAEAGALEMGPLIEERAVK
                     AVAEKVERAVKQGAKLVCGGKRAEGRGYFFEPTLLTDTDNSMDIMKEETFGPVLPVSA
                     FDTLDQVIALANDCEFGLTSSVYTTNLNEAFYVTRRLQFGETYINRENFEAMQGFHAG
                     WKKSGIGGADGKHGLEEYLQTQVVYLETDI"
     gene            complement(1681..1881)
                     /locus_tag="WIC03_000763"
     CDS             complement(1681..1881)
                     /locus_tag="WIC03_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883294.1"
                     /translation="MDTVGSLAIGYATGGNAGAFTGANADWNNRQLHSSEYKLAEQYA
                     DIVASRLNISKETCTVIYLGIN"
     gene            complement(1890..2279)
                     /locus_tag="WIC03_000764"
     CDS             complement(1890..2279)
                     /locus_tag="WIC03_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883295.1"
                     /translation="MLYKCKIKKLDNTIEEAVLIEINGYELHCFISYCPFKIFENQFY
                     EVELSYEIFNDYILDKSTSNIGFRKIDDSFKYEITAELKNNHLTLQNIQFEEDEYLIN
                     YSYLDNCLVTLTVDRISVSFIREIQAA"
     gene            complement(2488..3405)
                     /locus_tag="WIC03_000765"
     CDS             complement(2488..3405)
                     /locus_tag="WIC03_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ3883296.1"
                     /translation="MEKAEHLNSSRFVNLVKSGGGSYVEGSYRFDTLSNGISIHGGTV
                     TARCDFCSSRLAEPYVSFVLLLEGSLDFGINRCRFQIDADGGKIVLIAVGEEVLFSRY
                     LYRGGKTVKMTIKGMEQWLLRPEYARFAPLLYREPVRIWDLPPNLRGLAASCLKAVPK
                     GHLGETLRREADVLRLLSDLWDTVSDGIGPAAGQTAEADAMPSEDFSRTLNAAFADGA
                     HQVNRLTDALNISERTLQRRMRDHFGITASEWLHHKQMQHALYLLQNEGKSIGETAYL
                     CGYRHVSSFTQAFRQYFGSTPAETKKENR"
     gene            3578..4378
                     /locus_tag="WIC03_000766"
     CDS             3578..4378
                     /locus_tag="WIC03_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ3883297.1"
                     /translation="MSPSPFIEMKDVAFAYGDRPILKNINFSIPQGNFAAVMGGSGSG
                     KTTLMRLITGQIRPQSGQVLIEGRDLAGFSADELYEHRRRMGVLFQHGALFTDLSVFD
                     NIAFPMRELTRLPEAVIRDLVLLKLNAVGLRGVENLMPSELSGGMSRRVALARTIALD
                     PEIMLYDEPFTGLDPISLGVIAHLISRVNKALRSTSIMVTHDIEKSLEIVDQVIFLAH
                     GEIMFSGSPQEMRELDSPWVRQFVGGLADGPVAYRYPAQTSLQQDLLG"
     gene            4426..5202
                     /gene="mlaE"
                     /locus_tag="WIC03_000767"
     CDS             4426..5202
                     /gene="mlaE"
                     /locus_tag="WIC03_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HEZ3883298.1"
                     /translation="MNFIRSVGAKTLGLIQSLGSITLFLLNILAKSGTAFVRPRLSVR
                     QVYFAGVLSVLIVAVSGLFVGMVLGLQGYTQLSKFKSADILGYMVAASLLRELGPVLA
                     AILFASSAGGAMTSEIGLMKTTEQLEAMNVMAVNPVARVVAPRFWAGVFSMPLLASIF
                     NVAGIFGAYLVGVSWLGLDSGIFWSQMQNNITIHYDVINGLIKSAAFGVAVTLIAVHQ
                     GFHCVPTSEGILRASTRTVVSSALTILAVDFILTAWMFTD"
     gene            5253..5747
                     /gene="mlaD"
                     /locus_tag="WIC03_000768"
     CDS             5253..5747
                     /gene="mlaD"
                     /locus_tag="WIC03_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HEZ3883299.1"
                     /translation="MKKNILEFWVGLFVLLGVAAVGFLAFRVAGGAAFGNSGKTYTVY
                     ADFSDIGGLKANAPIKSAGVLVGRVGSIELDPKSHQAKVRLDLDSKYQFSSDVSAQIL
                     TSGLLGEQYIGLQQGGDTENLAAGDTISVTSSAMVLENLIGKFMTSFAEKNADGGNAE
                     KAAE"
     gene            5784..6374
                     /locus_tag="WIC03_000769"
     CDS             5784..6374
                     /locus_tag="WIC03_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HEZ3883300.1"
                     /translation="MKKSSLISALGIGILSIGMAFAAPADAVSQIRQNATQVLSILKN
                     GDANTARQKAEAYAIPYFDFQRMTALAVGNPWRTASDTQKQALAKEFQTLLIRTYSGT
                     MLKLKNANVNVKDNPIVNKGGKEIIVRAEVGVPGQKPVNMDFTTYQSGGKYRTYNVAI
                     EGASLVTVYRNQFGEIIKAKGVDGLIAELKAKNGGK"
     gene            6425..6703
                     /locus_tag="WIC03_000770"
     CDS             6425..6703
                     /locus_tag="WIC03_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HEZ3883301.1"
                     /translation="MHTELKNGILHIGGDITVKTLTAAAFTRFRQQCRLKDTCAVDLS
                     GVGRADSACVSLLLEVLRGCKGSVRLTGIPESVRALSELYEIKDWLKS"
     gene            6700..7527
                     /locus_tag="WIC03_000771"
     CDS             6700..7527
                     /locus_tag="WIC03_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HEZ3883302.1"
                     /translation="MKKTAYAFLLLIGFASAPAFAETRPADPYEGYNRAVFKFNDQAD
                     RYIFAPAARGYRKVAPKPVRAGVSNFFNNLCDVVSFGSNILRLDIKRASEDLVRVGIN
                     TTFGLGGLIDIAGAGGIPDNKNTLGDTFASWGWKNSNYFVLPVLGPSTVRDALGTGIT
                     SVYSPKNIVFRTPVGRWGTTAVSAVSTREGLLDLTDSLDEAAIDKYSYTRDLYMKVRA
                     RQTGATPAEGTEDNIDIDELVESAETGAAETAVQEDSVSETQAEAAGEAETQPGTQP"
     gene            7652..8014
                     /locus_tag="WIC03_000772"
     CDS             7652..8014
                     /locus_tag="WIC03_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ"
                     /protein_id="HEZ3883303.1"
                     /translation="MYEVNRSVFVLIPLEPFWNWLQTLPGNHLDGLTLEDIQADANSY
                     LVRPCETADEVWDEIETRFEDIFAAELADWCEDEREWPALDADIFNEWFDIQLSTVIT
                     DLEHEPLAREAFQPINLN"
     gene            8011..8397
                     /locus_tag="WIC03_000773"
     CDS             8011..8397
                     /locus_tag="WIC03_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEZ3883304.1"
                     /translation="MMKLTVRNYHLDGYGHVNNARYLEFFEEARWAFFEERGLLHELA
                     GLILIVARIDIRYSRPAVEGDVLQFSCRLKTPGTRRIVLTQTITLPNGKTAAEADITL
                     MPVHAATQRTVSLPATLARALEALSE"
     gene            8394..8882
                     /locus_tag="WIC03_000774"
     CDS             8394..8882
                     /locus_tag="WIC03_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEZ3883305.1"
                     /translation="MKKILTAAVVALIGILLAIVLIPDSKTAPAFSLSDLHGKTVSNA
                     DLQGKVTLINFWFPSCPGCVSEMPKIIKTANDYKNKNFQVLAVAQPIDPIESVRQYVK
                     DYGLPFTVMYDADKAVGQAFGTQVYPTSVLIGKKGEILKTYVGEPDFGKLYQEIDTAL
                     AQ"
     gene            9391..9606
                     /gene="rpmE"
                     /locus_tag="WIC03_000775"
     CDS             9391..9606
                     /gene="rpmE"
                     /locus_tag="WIC03_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEZ3883306.1"
                     /translation="MKQGIHPNYHEVNVTCSCGNKFATKSAMEKENFNIEVCSLCHPF
                     YTGTQKIVDTTGRVDKFNNKFGNLFKR"
BASE COUNT         2237 a         2681 c         2425 g         2343 t
ORIGIN      
        1 acgattctct aaggtgctca agcaccgagt gaatcggttc cgtactatct gtactgtctg
       61 cggcttcgtt taaatttaat ccgctataca aagacaaaaa catcgacctc ggtcatgact
      121 gattgccgat gaagcaataa aaatgccgtc tgaacctgaa aacgggttca gacggcattt
      181 tctatcgggg ttttaaagcg gcattaaatg tcggtttcca aataaacgac ttgggtttgc
      241 agatattctt ccaaaccgtg tttgccgtcc gcgccgccga taccggattt tttccaaccg
      301 gcgtggaaac cctgcatggc ttcgaagttt tcgcggttga tgtaggtttc gccgaattgc
      361 aggcggcggg taacgtagaa ggcttcgttt aaattagtcg tataaacaga actggtcaga
      421 ccaaactcgc aatcgttagc cagtgcgatg acttggtcga gcgtgtcgaa agcggaaacg
      481 ggcagcacgg ggccgaaggt ttcttctttc ataatgtcca tactgttgtc ggtgtcggtc
      541 agcagggtcg gctcgaagaa atagccgcgc ccttcggcgc gtttgccgcc gcagaccagt
      601 ttcgcacctt gtttgactgc ccgttccact ttttcggcaa cggctttgac ggcgcgctct
      661 tcaatcagcg gacccatttc cagcgcgcct gcttcggctt cggcagggtt gccgtagcgc
      721 acgcctttca tggcggcggt cattttttca atgaacgcgt ctttcaggct gctgtggaca
      781 tagacgcgct cggcgcagtt gcagatttga ccggtgttgc cgacgcgcga agccaagatg
      841 gatttcaccg ccaaatccaa atccgcgtct ttcaaaacga tggcgggggc tttgccgccg
      901 agttccagcg aaactttggt gatgttggcg gaagcggctt ccatcacttg gcggcctgct
      961 tcgacggagc cggtcaggct gaccatatcg acttgcggat gggcagacaa ggcattgccg
     1021 atttccgcac ccggaccgtt caccacgttg aacacgcctg cgggcaatcc gaccgcatcg
     1081 acgatttcgg cgaagatgtg gcagttgatc ggggttacgc tgctgggttt gacgacgatg
     1141 gtgttgcccg ttaccaaagc ggggcccatt ttgcgggcaa tcaggaagaa ggggaagttc
     1201 cacggcaaaa tgccggcgat tacgcccagc ggacgtttga acaataaaat gttttcgcgc
     1261 gggcggtcgc tttggatgat ttcgccttcg taacggcgcg cccattcggc ctggtaatcg
     1321 agataatcgg cggtaaacat gacttccacg cgcgccaaat ctttggtttt gccaccttcg
     1381 gctacgatgg tgtcggtcag ctcgtcggcg cgtccgcgta tgccttgggc gattttgcgc
     1441 aaatacgcgc cgcgttcgac cgcaggcagg cgttcccaag ccggttgcgc cgcgcgtgcc
     1501 gccgccacag ctcggtcaac gtctgctttt ccgcctttgg gttcgcgggc gatggtttct
     1561 tcggtggaag ggttcaatac gtcgcgccat tcgccgttga agtcgttttc aaagcgtccg
     1621 ttgatgtaca tggccaattg tttcatttcg ggttctccag ttttgtagtc agatgtagtt
     1681 ttagtttatt cccaaataaa ttaccgtaca agtttctttt gaaatgttta gacgagaagc
     1741 tactatgtct gcatattgtt ctgctaattt atattcactc gaatgcaact gccgattatt
     1801 ccaatctgca ttcgcaccag taaaagcccc agcattgccg cctgtggcat aaccaatcgc
     1861 taaactgcca acggtatcca caacaggttt caggctgcct gaatttctct aataaatgaa
     1921 acacttattc tatcaaccgt taatgtaacc aaacaattat caagataaga gtagtttatt
     1981 aaatactcat cttcttcaaa ttgtatattt tgtagggtta gatgattatt ttttaattct
     2041 gccgttattt catacttgaa actatcatct atttttctaa atccaatatt tgaagttgat
     2101 ttatccagta tgtaatcatt aaagatttca taggataatt ccacttcata aaattgattt
     2161 tcaaatattt tgaatgggca gtatgatata aaacaatgta gttcataacc atttatttca
     2221 atcaatactg cctcttcaat ggtgttatct aactttttga ttttacattt atataacatt
     2281 ttatcttgcc tttagataaa ttgttttgtt ttttggattg ttattcaaag ttctccaaaa
     2341 gtcttttcct gattttgtat gtatcgtggt aacactacca ttttcattgg ttccaacaaa
     2401 aataaattta gattgacctt tggaatcagt acccaaaaac tgaacatatc cagacatgca
     2461 gatttcgggt tttaaatcaa atgcggctta ccggttttct tttttggttt ccgccggcgt
     2521 gctgccgaaa tattgcctga atgcctgagt aaagctggaa acgtggcggt agccgcataa
     2581 atatgcggtt tcgcctatgc ttttcccctc gttttgcaac aggtagagcg cgtgctgcat
     2641 ttgtttgtgg tgcagccact cgcttgcggt aataccgaaa tggtcgcgca tacggcgttg
     2701 cagcgtcctt tcactgatgt tcagcgcgtc tgtcagccgg ttgacttggt gtgcgccgtc
     2761 ggcaaacgcg gcatttaggg tgcggctgaa gtcttcagac ggcatagcgt ctgcttccgc
     2821 cgtttgcccc gccgccggcc cgatgccgtc tgaaaccgtg tcccacaagt ccgacagcag
     2881 ccgcaacacg tccgcctcgc ggcgcaatgt ttcgcccaaa tgcccctttg ggacggcttt
     2941 caggcaggat gccgccaagc cgcgcaggtt cgggggcaaa tcccatatcc tgaccggttc
     3001 gcggtaaagc aggggtgcga aacgcgcgta ttccggacgc agcagccatt gttccatacc
     3061 tttaattgtc attttgaccg ttttgccgcc tcggtaaaga tagcggctga acaggacttc
     3121 ttccccgaca gcaattagga caatcttgcc gccgtccgca tcgatttgga agcggcagcg
     3181 gttgatgccg aagtccaaac tgccttccag caagagcacg aacgacacat aaggttcggc
     3241 gaggcggctg ctgcaaaaat cacaccgtgc cgttactgtg ccgccgtgga tggaaatgcc
     3301 gttggacaaa gtatcgaaac ggtagctgcc ctccacatag ctgccgccgc cgcttttgac
     3361 tagattgacg aaccggctgc tgttcaaatg ttctgctttt tccatagttt tctgataaag
     3421 gttatcattt gaaagataac atttttcgcc acagcaaaca atccgccgcc gacaacggca
     3481 ttttcacaac atttaccgtc cgtatgccgt ctgaaaattg cccgtcttcc cgataagcct
     3541 taaaatacac gccgtcaaac ctaccgtccg tccgtacatg agcccatccc cctttatcga
     3601 aatgaaagac gtcgccttcg cgtatggcga ccgcccgatt ctgaagaaca tcaatttcag
     3661 cattccgcaa ggcaattttg ctgccgtaat gggcggttcg ggcagcggca aaaccacgct
     3721 gatgcgactg attacaggac agattcgtcc gcagtccggg caggttttga ttgaaggacg
     3781 ggatttggcg ggcttttcgg ctgacgagtt gtatgaacac cgccgccgta tgggcgtatt
     3841 gttccaacac ggcgcgctgt ttactgattt gtcggtattc gacaatatcg cttttccgat
     3901 gcgcgaactg acgcgcctgc cggaagcggt gattcgagat ttggttttgt tgaaactgaa
     3961 cgcggtcggg ctgcgcggcg tggaaaacct gatgccgtcc gagttgtccg gcgggatgtc
     4021 gcgccgcgtc gcgcttgccc gcacaatcgc gctcgatccc gaaattatgt tgtacgacga
     4081 gccgtttacc ggtctcgatc cgatttcctt gggcgtgatt gcccacttga tcagccgcgt
     4141 caacaaggct ttgcgttcga ccagtattat ggtcacgcac gacattgaaa aatctttgga
     4201 aatcgtcgat caggtgattt tcttggcgca cggcgaaatt atgttctccg gctcgccgca
     4261 ggaaatgcgc gaactggatt cgccttgggt gcgccagttt gtcggcggct tggcagacgg
     4321 ccccgtggca taccgttatc cggcgcaaac gtcgttgcag caggatttgc tcgggtaaat
     4381 gagccggtgc cgtctgaaac cgttgaactt tacatataga aatttatgaa ctttatccgt
     4441 tccgtcgggg cgaaaaccct cggccttatt caatccctcg gcagtattac gctgtttctg
     4501 ctgaacatct tggcgaaatc cggtacggct ttcgtccgtc cgcgcctgag cgtgcgccaa
     4561 gtgtatttcg ccggcgtgct gtcggtgctg attgttgccg tttcggggct gttcgtcggt
     4621 atggttttgg gtttgcaggg ttatacgcag ttgtcgaaat tcaaatccgc cgatattttg
     4681 ggctatatgg tcgcggcttc gctattgcgc gagctgggtc cggtattggc agcgattttg
     4741 tttgccagca gcgcgggcgg tgcgatgacc agcgaaatcg gtctgatgaa aacgaccgaa
     4801 cagctcgaag cgatgaatgt gatggcggta aaccctgtcg cccgcgtggt tgcgccgcgc
     4861 ttttgggcgg gcgtgttttc catgccgctt ttggcttcga ttttcaacgt ggcgggcatt
     4921 ttcggtgcgt atttggtcgg tgtaagctgg ctgggcttgg acagcggcat tttctggtcg
     4981 caaatgcaga acaacatcac gatacattac gatgtaatca acggtctgat taaatccgcc
     5041 gcgttcggcg tggcggtaac gctgattgcc gtgcatcagg gcttccattg cgtcccgact
     5101 tcggaaggca ttttgcgcgc cagcacgcgc acggtggttt cgtccgccct gacgattttg
     5161 gcggtcgatt ttatattgac cgcgtggatg tttacagatt gacaggccgt ctgaagacga
     5221 aacgcggaac atcggatatt caaggaactt taatgaaaaa gaacatattg gaattttggg
     5281 tcggactgtt tgtcctgctc ggcgtggcag cggtcggctt tctcgctttc cgcgtggcag
     5341 gcggcgcggc cttcggcaat tccggcaaga cttataccgt atatgccgat ttcagcgaca
     5401 tcggcggact gaaggccaac gcgccgatca aatccgcagg cgtattggtc ggacgcgttg
     5461 gttccatcga gcttgacccg aaatcccatc aggcgaaagt ccgtctcgat ttggacagca
     5521 aatatcagtt cagtagcgac gtttccgcac aaatcctgac ttcgggactt ttgggcgagc
     5581 agtacatcgg gctgcagcag ggcggcgaca cggaaaacct tgctgccggc gacaccatct
     5641 ccgtaaccag ttctgcaatg gttctggaaa accttatcgg caaattcatg acgagttttg
     5701 ccgagaaaaa tgccgacggc ggcaatgcgg aaaaagccgc cgaataaacg tatgccatca
     5761 tattcaaact aggacactta atcatgaaaa aatcctccct catcagcgca ttgggcatcg
     5821 gtattttgag catcggcatg gcatttgccg cccctgccga cgcggtaagc caaatccgtc
     5881 aaaacgccac tcaagtattg agcatcttaa aaaacggcga tgccaacacc gcccgccaaa
     5941 aagccgaagc ctatgcgatt ccctatttcg atttccaacg tatgaccgca ttggcggtcg
     6001 gcaacccttg gcgcaccgcg tccgacacgc aaaaacaagc gttggccaaa gaatttcaaa
     6061 ccctgctgat ccgcacctat tccggcacga tgctgaaatt aaaaaacgcc aacgtcaacg
     6121 tcaaagacaa tcccatcgtc aataaaggcg gcaaagaaat catcgtccgc gccgaagtcg
     6181 gcgtacccgg gcaaaaaccc gtcaacatgg acttcaccac ctaccaaagc ggcggtaaat
     6241 accgtaccta caacgtcgcc atcgaaggcg cgagcctggt taccgtgtac cgcaaccaat
     6301 tcggcgaaat tatcaaagcg aaaggcgtgg acggactgat tgccgagttg aaagccaaaa
     6361 acggcggcaa ataaccgaca aaccataccg tccgagggcg gtatgggttt tccgagcaaa
     6421 cgctatgcat acagaactca aaaacggaat attgcacatc ggcggcgaca tcaccgtcaa
     6481 aaccctgacc gccgccgctt ttacccgctt cagacagcaa tgtcgtctga aagacacctg
     6541 cgcggttgat ttgagcggcg tcggacgcgc cgattccgcc tgcgtgtcgc tgctgctcga
     6601 agtgctgcgc ggttgcaaag gcagcgtcag gctgaccggc attcccgaat ccgtgcgcgc
     6661 gctgtccgaa ctgtacgaaa tcaaagactg gctgaaatca tgaaaaaaac cgcctatgcc
     6721 ttcctcctgc tgatcgggtt cgcttccgcc cctgcatttg ccgaaacccg ccccgccgac
     6781 ccttatgaag gctacaaccg cgccgttttc aaattcaacg accaagccga ccgctacatt
     6841 ttcgcccctg ccgcgcgcgg ctaccgcaaa gttgcgccga aacccgtccg cgccggcgtg
     6901 tccaattttt ttaacaacct gtgcgacgtg gtcagcttcg gtagcaatat cttgcgcttg
     6961 gacatcaaac gcgcaagcga agaccttgtc cgcgtcggca tcaacaccac tttcggtttg
     7021 ggcgggctta tcgacatcgc cggcgcgggc ggcattcccg acaataaaaa caccttgggc
     7081 gacacgtttg cttcgtgggg atggaaaaac agcaattatt tcgtgttgcc cgtcttaggg
     7141 ccgtccaccg tccgcgacgc gctcggcacg ggtattacct ccgtttattc gcccaagaat
     7201 atcgtcttcc gcacccctgt cggacgctgg ggcacgactg ccgtatccgc cgtcagtacg
     7261 cgcgaaggcc tgctcgattt gaccgacagt ctggacgaag ccgccatcga caaatacagc
     7321 tacacgcgcg acctctatat gaaagtccgt gcgcggcaga ccggtgcaac acctgccgaa
     7381 ggtacggaag ataacatcga catcgacgaa ttggtcgaaa gtgccgaaac cggcgcggcg
     7441 gaaactgccg ttcaagaaga ttccgtatcc gaaacacagg cagaagcagc aggggaagcc
     7501 gaaacgcaac ctggaacaca accttaaagc catgccgtct gaaaatccgt gttcggacgg
     7561 cattcccttc tgtcggaacg gacagtccga aaccacagat tgggctataa tctctttttt
     7621 gcgcataccg cgcgacagca aaggaaaaac catgtacgaa gtcaaccgca gcgtattcgt
     7681 cctcatccct ttagaacctt tttggaactg gctgcaaacc ctgccgggca accaccttga
     7741 cggactgacc ctcgaagaca tccaagccga cgcgaattcc taccttgtcc gcccgtgcga
     7801 aaccgccgac gaagtatggg acgaaatcga aacccgtttt gaagacattt tcgccgccga
     7861 acttgccgac tggtgcgaag acgaacgcga gtggcctgcg ctcgatgccg acattttcaa
     7921 cgaatggttc gacatccagc tttctaccgt catcaccgac ctcgaacacg aaccgcttgc
     7981 ccgcgaagcc ttccaaccca tcaacctgaa ctgatgaagc tgaccgtccg caactaccat
     8041 ctcgacggct acggccatgt caacaatgcg cgctacctcg aatttttcga agaagcgcgc
     8101 tgggcgtttt ttgaagaacg cggactgctg cacgagttgg caggcctcat actgattgtc
     8161 gcccgcatcg acatccgata cagccgcccc gccgtcgaag gcgacgtatt gcagttttca
     8221 tgccgcctga aaacccccgg cacgcgccgt attgtgctga cccagacgat tacactgcca
     8281 aacggcaaaa ccgccgcaga agccgacatc accctgatgc ccgtccacgc cgccacacaa
     8341 cgcaccgtca gcctgcccgc cacccttgcc cgcgcattgg aagccttgtc cgaatgaaaa
     8401 aaatactcac cgccgccgtc gtcgcactga tcggcatcct ccttgccatc gtcctcatcc
     8461 ccgacagcaa aaccgcgccc gccttctccc tgtccgacct gcacggaaaa accgtttcca
     8521 acgccgacct gcaaggcaaa gttaccctga ttaatttttg gtttccctcc tgtccgggtt
     8581 gtgtaagcga aatgcccaaa atcattaaaa cggcaaatga ctataaaaac aaaaacttcc
     8641 aagtccttgc cgtcgcccag cccatcgatc cgatagaaag cgtccgccaa tatgtcaaag
     8701 actacggttt gccgtttacc gtcatgtatg atgcggacaa agctgtcgga caggcgttcg
     8761 gcacacaggt ttatccgact tccgtcctta tcggcaaaaa aggcgaaatc ctcaaaactt
     8821 atgtcggcga acccgatttc ggcaaactct accaagaaat cgataccgcg ctggcgcaat
     8881 aaacacattt gccctgccgt ccgaacgcaa aggaagggat gcgggcgcgt tcctgctttt
     8941 tgcatgttcg gcttcagacg gcatttcatc ttccggcggt tttttaccgt tgccggactg
     9001 cccgaagcca aagggcgggg gatgcctttt gcaggcgcac ccgaaccgcg tgccggtacg
     9061 atgcccctcc gtccctagac tgcctcccgg caaaacggcg gattccgtac cgcctttaaa
     9121 gcgtttggct gtcaaacttg ccattgctcg aataaaaaaa tattccttcc gccgttatct
     9181 ttcccggcag ccggcttcgg gcgcattccg tccggggcgg cactttatgc gccgtcttgt
     9241 gccgaaacgc cccataaagc cccctctggc acggactttt tacattttat cgaatatcct
     9301 cttgacgaaa caacagaaaa tagcgagaat gccgagcttg tctgaagcat aggtacatcc
     9361 cctatgcccg cttttttgaa aggaaatcaa atgaaacaag gtattcaccc gaactaccac
     9421 gaagtcaacg ttacctgctc ttgcggcaac aaattcgcaa ccaaatccgc gatggaaaaa
     9481 gaaaacttca atatcgaggt ttgctccctg tgccacccgt tctataccgg cacccaaaaa
     9541 atcgttgata ccaccggccg cgtggacaaa ttcaacaaca aattcggcaa cctgttcaaa
     9601 cgctgattgc cgttttgcaa gaattaagcc ctgccatcat ttcgatggca gggcttttta
     9661 atatttggca cactgacttc ttatct
//
LOCUS       DAWHSK010000047         9681 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.047, whole genome shotgun sequence.
ACCESSION   DAWHSK010000047 DAWHSK010000000
VERSION     DAWHSK010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9681
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.047"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            117..1292
                     /locus_tag="WIC03_000776"
     CDS             117..1292
                     /locus_tag="WIC03_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEZ3883307.1"
                     /translation="MLNTSLSPWPCFTQEEADAVSKVLLSNKVNYWTGSECRKFEKEF
                     AAFAGTRYAVALSNGTLALDVALKAIGIGAGNDVIVTSRTFLASASCIVNAGANPVFA
                     DVDLNSQNISAETIKAALTSNTKAIIVVHLAGMPAEMDGIMDLAKEHDLWVIEDCAQA
                     HGAKYKGKSVGSIGHVGAWSFCQDKIMTTGGEGGMVTTNDKTLWEKMWSYKDHGKSYD
                     AVYHREHAPGFRWLHESFGTNWRMMEMQAAIGRIQLKRLPEWTACRQAHAAKLAESLG
                     KFKSIRLVEVADYIGHAQYKFYAFVKPEHLKDGWTRDRIVSELNARKVPCYQGSCSEV
                     YLEKSFDDTPWRPKERLKNAVELGDTSLMFLVHPTLTDDEIAFCKEHIEAVLAEATR"
     gene            1340..3250
                     /gene="pglD"
                     /locus_tag="WIC03_000777"
     CDS             1340..3250
                     /gene="pglD"
                     /locus_tag="WIC03_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent dehydratase PglD"
                     /protein_id="HEZ3883308.1"
                     /translation="MTLETLIALPRNIKKICFLIHDFLMIFIAFWFTQSLKADYSDEW
                     FDPANWQSFLLTAFLTACLFFRMGLYRAVTRFISFRILSTALAGSLASAVLFFLNTLI
                     FEERLRLALPIVYFLLLFVSVTGSRMVLRGLLSEHPKKQMAPVIIYGAGRSGRQLLKA
                     VKQMREYSAAAFVDDDPKLWHTVIYDLAVYQPDAIAFLIERYGVEKILLAIPGATQEQ
                     RLRIINKLEAYPCEVLTIPGMKDLMDGKISIGTLKKISVSDLLGRDSVAPDDRLMNAD
                     IEGKTVMVTGAGGSIGSELCRQIIRRRPEKLLLFELSEFALYAIEKELRETCIQKRLD
                     TEILPFLGSVQNRTLLEHVMTAFSVATVYHAAAYKHVPMVEFNTVEGIRNNIFGTLEC
                     ALAATASGVRTFVLISTDKAVRPTNTMGAGKRMAELCLQALAAEPGQKTRFSMVRFGN
                     VLGSSGSVVPLFEKQIAEGGPLTLTHPEITRYFMTIPEAAQLVIQAGAMGTGGDVFVL
                     DMGESVKIIDLARQMITLSGLKPKTPEQPDGDIEILITGLRPGEKLYEELLIGDNVRK
                     TGHPRIMTADEAMLPWHELSVLLDRIRAACDRYDQQAIRTLLINAPTGFTPSDGICDL
                     LWVRETHRKNAV"
     gene            3306..4598
                     /locus_tag="WIC03_000778"
     CDS             3306..4598
                     /locus_tag="WIC03_000778"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HEZ3883309.1"
                     /translation="MQFSAFGEKFTQHSGILQLMDNLGDALKSDKPVNMLGGGNPARI
                     PEIDRAFADIFSKLAAEHAVENIGNYSNPQGDAVLIDALTAFLNREYSWNLTADNIAL
                     TNGSQNAFFYLFNLFGGKFKLSDGTSAEKAILLPLAPEYIGYADVHVEGQHFVSVKPK
                     IENVEHEGEAGFFKYRVDFDALENLPELKAGKIGAICCSRPTNPTGNVLTDGEMARLD
                     ALAREHGIPLIIDNAYGMPFPNIIYSDVTLNWHENIILCFSLSKVGLPGVRTGIIVAA
                     PEVVKAVSSLNAIVNLAPTRFGAAIAAPLLESGEMKRLADQVIRPFYRNQAQTAVSLL
                     KRELGAYPMKIHKPEGAIFLWLWFENLPVSSQTLYEMLKAEGTLIIPGEHFFVGIDTQ
                     DYPHAGECIRMSIAQDAQTLEKGIAAIGKTVRKLYDNV"
     gene            complement(4650..5486)
                     /locus_tag="WIC03_000779"
     CDS             complement(4650..5486)
                     /locus_tag="WIC03_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEZ3883310.1"
                     /translation="MIDLHCHSTVSDGMLSPAEIVRLAHQNGCTLLALTDHDHTGGIA
                     EARAEADRLGLRLINGVEISVTWRGRTIHVVGLDFDEQDENLQNLLAQVRQGRLKRLE
                     AIADKLEKKGIGGAYDGALELAANKEMVSRTHVAEFLIKNGYVKNKQQAFTKYLGDGK
                     SCAVRHEWATLEDCVSAVNGAGGLAVIAHPMRYDLSATAKRNLFEEFKNLGGAGIEVH
                     SGNCCKNDRLNYALLAERFGMLASAGSDFHRLNDFSGGILGACPELPENCRPVWAHFS
                     RH"
     gene            5495..5737
                     /locus_tag="WIC03_000780"
     CDS             5495..5737
                     /locus_tag="WIC03_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883311.1"
                     /translation="MCVVNGGQGVSILSGGVAAVISKNKCMANAKEKPLCRHSRAGGN
                     PDFDLLEMFKVNRYFKLPDSCFRGNGDMTVFNFNLL"
     gene            complement(5779..5988)
                     /locus_tag="WIC03_000781"
     CDS             complement(5779..5988)
                     /locus_tag="WIC03_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883312.1"
                     /translation="MDMTLKHKAEALLGEPLLDEPVRPESWECCGSDCGEACIQTIYW
                     ADKARYDAQRRKLKEAGWAEDENAV"
     gene            complement(5966..6199)
                     /locus_tag="WIC03_000782"
     CDS             complement(5966..6199)
                     /locus_tag="WIC03_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEZ3883313.1"
                     /translation="MEATVYLEDNEYIALCDLLKLAGLAESGGQAKALIAEGLVSRNG
                     ETETRKTAKIRGGEVIEFDGARLEIADGYDPEA"
     gene            6738..>9681
                     /gene="dnaE"
                     /locus_tag="WIC03_000783"
     CDS             6738..>9681
                     /gene="dnaE"
                     /locus_tag="WIC03_000783"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEZ3883314.1"
                     /translation="MTEPTYIPLRLHTEFSITDGMVRIKKLIAKAQEYGLPALGISDL
                     MNEFGLVKFYKACRGAGIKPIGAADVWIGNPNAPDKPFRAMLIIRNDAGYLRLSELLT
                     EAYVGKDRNIHHAELNPEWLENGDNSGLICLSGAHYGEVGMNLLNGNEDAARAAALKY
                     AAWFPDAFYLELQRLPERPEWEACVSGSVKLAEELGLPVVATHPTQFMSRDDFNAHEA
                     RVCIAGGWVLTDKKRPRDFTPGQFFIPPETMAERFADLPEALENTVEIAKRCNLHITL
                     GKNFLPLFPTPDGLSLDDYLIKLSNEGLQERMVQLYPDEAERAAKMPEYQKRLDFELN
                     IIIQMKFPGYFLIVQDFINWAKTHGCPVGPGRGSGAGSLVAYSLKITDLDPLKYALLF
                     ERFLNPERVSMPDFDVDFCQSNRGRVIEYVREKYGAEAVSQIVTFGTMSSKAVIRDVG
                     RVLELPFMLCDKLSKLIPLEANKPLSLEKAMETEPQIQELIEAEEADELITLAKKLED
                     LTRGLGMHAGGVLIAPGKISDYSPVYQADESASPVSMYDKGDVEDVGLVKFDFLGLRN
                     LTIIEMAQDNIKNTTGDIIDVGKIPLDDQVAYQIFRDANTTAVFQFESTGMKKMLKTA
                     HTTKFEELIAFVSLYRPGPMDNIPDFVARMKGQEFQYIHPLLEGILAPTYGIMVYQEQ
                     VMQAAQIIGGYSLGGADLLRRAMGKKKPEEMVKHREIFAEGAAKQGISREKSDEIFNY
                     MEKFAGYGFNKSHAAAYALISYQTAWLKAHYPAEFMAATMSSELDNTDQLKHFYDDCR
                     ANGIEFLPPDINESDYRFTPYPDMKIRYALGAIKGTGEAAVESIIAARQSGGKFTGLL
                     DFCERVGKEHMNRRTLEALIRGGAFDSIEPNRAMLLANIDLAMDNADQKAANANQGGL
                     FDMMEDAIEPVRLIDAPMWSESEKLAEEKTVIGFYLSGHPFGPYAQEVRQIAPQKLSK
                     LKPQDSVRL"
BASE COUNT         2299 a         2924 c         2472 g         1986 t
ORIGIN      
        1 ccccgtacag accgattttg acaacacctg cggcgcgcgc ccgattcttc ggaacacgcc
       61 ccccttcaga cggcataggg tcggaaatgc cgtctgaacc cgacggacca aacaccatgc
      121 tgaacacctc cctctccccg tggccctgct ttacccaaga agaagccgat gccgtttcca
      181 aagtcctgct gtccaacaaa gtcaactact ggacgggcag cgaatgccgc aaatttgaaa
      241 aagaatttgc cgcctttgcc ggcacgcggt acgccgtcgc cctttccaac ggcacgctgg
      301 cactcgatgt cgcgctcaaa gcaatcggca taggcgcggg caacgatgtg attgttacct
      361 cgcgcacttt cttggcttcc gcttcctgca ttgtgaacgc gggcgcaaat cccgtgtttg
      421 ccgatgtgga tttgaacagc caaaacatca gcgccgaaac tatcaaagcc gcgctgacgt
      481 cgaataccaa agccatcatc gtcgtccacc tcgccggtat gcccgccgaa atggacggca
      541 tcatggactt ggcaaaagaa catgatttgt gggtcatcga agactgcgcc caagcgcacg
      601 gcgcgaaata caaaggcaaa tccgtcggca gcatcggaca cgtcggcgca tggtcgttct
      661 gccaagacaa aatcatgacc acgggcggcg aaggcggcat ggttacgacc aacgacaaaa
      721 ccctgtggga aaaaatgtgg tcgtacaaag accacggcaa aagctacgat gccgtgtacc
      781 accgcgaaca cgcgcccggt ttccgctggc tgcacgaaag tttcggcaca aactggcgta
      841 tgatggaaat gcaggcggca atcgggcgca tccagctcaa acgcctgccc gaatggactg
      901 cctgccgtca agcgcacgcc gccaagctgg cggaaagcct aggcaaattc aaaagtatcc
      961 gcttggttga agtcgccgac tacatcggac acgcgcaata taagttctac gctttcgtca
     1021 aacccgaaca cctcaaagac ggctggacgc gcgaccgcat cgtcagcgaa ctgaacgcgc
     1081 gcaaagtccc ctgctatcaa ggcagctgct ccgaagtcta tttggaaaaa tctttcgacg
     1141 acacgccttg gcggccgaaa gagcgtttga aaaatgctgt cgaactgggc gacaccagcc
     1201 tgatgttctt ggtgcacccg acgctgaccg acgacgaaat cgcgttctgc aaagaacaca
     1261 tcgaagccgt attggcagaa gccacacgat aaccattcag acggcatatg ccgtctgaaa
     1321 aatacacgcc gccgacaaca tgactctgga aaccctgatc gccctgccgc gcaacatcaa
     1381 gaaaatctgt ttcctcatac acgattttct gatgattttc attgcctttt ggttcaccca
     1441 aagcctaaag gccgactact cggacgaatg gttcgacccc gccaactggc agtctttcct
     1501 attgaccgcg tttttgaccg cctgcctgtt cttcagaatg gggctttacc gcgccgtcac
     1561 acgcttcatc agcttccgca tcctctccac cgcgctggca ggcagcctcg cctccgccgt
     1621 attgtttttc ctcaatacgc tgatatttga agaacggctg cggctcgccc tgccgattgt
     1681 ctatttctta ctgctgtttg tttccgtgac cggctcgcgt atggttttgc gcggactgtt
     1741 gtccgaacac cccaaaaaac agatggcccc cgtcattatt tacggcgcgg gacggtcggg
     1801 cagacaactg cttaaggccg tcaaacaaat gcgcgaatat tccgccgccg cctttgtgga
     1861 cgacgacccc aaactgtggc acaccgtcat ctacgacctt gccgtttacc agcccgatgc
     1921 catcgccttc ctcatcgaac gctacggcgt ggaaaaaatc ctgctcgcca tccccggcgc
     1981 gactcaggaa caacgcctcc gaatcatcaa caaactggaa gcctatccct gcgaagtgtt
     2041 gaccattccc ggaatgaaag acctgatgga cggaaaaatc agcatcggca cgctcaaaaa
     2101 aatctctgtg tccgacctgc tcgggcgtga ttccgtcgca ccagacgacc gcctcatgaa
     2161 tgccgacatc gaaggcaaaa ccgtcatggt aaccggcgcg ggcggctcca tcggttcgga
     2221 actctgccgc cagattatcc gccgccgccc cgaaaagctg ctgctgttcg agttatccga
     2281 attcgccctg tacgccatcg aaaaagaatt gcgcgaaacc tgcatccaaa aacgcctcga
     2341 taccgaaatc ctgccctttc tcggttcggt gcaaaaccgc acgctgctcg aacacgtcat
     2401 gaccgccttt tccgttgcga ccgtctatca cgccgccgcc tacaaacacg tccccatggt
     2461 cgagttcaac accgtcgaag gcatacgcaa caacatcttc ggcacactcg agtgcgcgct
     2521 tgccgccacg gcatcgggcg taagaacttt cgtcctcatc tccaccgaca aagccgtccg
     2581 ccccaccaac accatgggtg ccggcaaacg catggcggaa ctctgccttc aagcgctcgc
     2641 cgccgaaccc ggacaaaaaa cccgcttcag catggtgcgt ttcggcaatg ttttaggttc
     2701 gtccggctcc gttgtcccgc tgtttgaaaa acagattgcc gaaggcggcc cgcttaccct
     2761 gacccaccct gaaatcacac gatatttcat gaccataccc gaagccgccc aactcgtcat
     2821 acaggcaggt gcgatgggta cgggcggcga cgtattcgtc ctcgacatgg gcgaatccgt
     2881 caaaatcatc gaccttgccc gccaaatgat taccttaagc ggcctcaaac ccaaaacacc
     2941 cgaacaaccc gacggcgaca tcgaaatcct cattaccgga ctgcgtcccg gagaaaaact
     3001 ctacgaagag ctgctcatcg gcgacaacgt ccgcaaaacc ggccatccgc gcattatgac
     3061 ggctgacgag gcgatgctgc cgtggcacga gctctctgtc ctgctcgacc gcatccgtgc
     3121 ggcctgcgac cgttacgacc agcaggcaat ccgcaccctg ctcataaacg ccccgaccgg
     3181 ctttacccca agcgacggta tctgcgacct gctttgggta cgagaaacac acagaaaaaa
     3241 tgccgtctga accttcagac ggcataacgt acaaaccaac ctaccttaca cacacggagt
     3301 ttgacatgca gttttcagca ttcggcgaaa aattcacgca acacagcggc atcctccaac
     3361 taatggacaa cctcggcgac gcgctcaaaa gcgacaagcc cgtcaacatg ctcggcggcg
     3421 gcaatccggc gcgcattccg gaaatcgatc gggcgtttgc cgacatattt tccaaactgg
     3481 cggcagaaca cgccgtcgaa aacatcggca actactccaa tccccaaggc gatgccgtgc
     3541 tgattgacgc gctgaccgcc ttcctcaacc gcgaatacag ctggaatctg accgccgaca
     3601 atatcgcgct gaccaacggt tcgcaaaacg cgtttttcta tcttttcaac ctcttcggcg
     3661 gcaaattcaa gctttcagac ggcacatccg cagaaaaagc cattttgttg ccgctcgcgc
     3721 ccgaatacat cggctatgcc gacgtgcatg tcgaagggca gcacttcgtt tccgtcaagc
     3781 ccaaaatcga aaacgtcgaa cacgaaggcg aagcaggctt cttcaaatac cgcgtggact
     3841 ttgacgcact ggaaaacctg cccgaactca aagcgggcaa aatcggcgcg atttgctgtt
     3901 cgcgcccgac caacccgacc ggcaatgtgt tgaccgacgg cgaaatggcg cgtttggacg
     3961 ctttggcgcg tgaacacggg attccgctga ttatcgacaa cgcctacgga atgccgttcc
     4021 ccaacatcat ttacagcgac gtaacgctga attggcatga aaacatcatc ctctgcttca
     4081 gcctttccaa agtcggcctg ccgggcgttc gcaccggcat catcgtcgcc gcgccggaag
     4141 tggtcaaagc cgtcagcagc ctgaacgcga ttgtgaacct tgcccccacg cgcttcggcg
     4201 cggccatcgc cgccccgcta ctggaaagcg gcgagatgaa acggcttgcc gaccaagtca
     4261 tccggccgtt ttaccgcaat caggcgcaaa ccgccgtctc gctgctcaag cgcgagctgg
     4321 gcgcgtaccc gatgaaaatc cacaaacccg aaggcgcgat tttcctgtgg ctctggtttg
     4381 aaaacctgcc cgtttcttcg caaaccctgt acgaaatgct caaagccgaa ggtacgctga
     4441 ttattccggg cgaacatttc ttcgtcggca tcgacacgca ggattacccg cacgcgggcg
     4501 agtgcatccg catgagcatc gcgcaggacg ctcaaacgct ggaaaaaggc attgccgcca
     4561 tcggcaaaac cgtccgaaaa ctgtacgaca acgtttaaac cgcaaaaaat gccgtctgaa
     4621 aggttttcag acggcatttt tatctgcatt caatgtcggg aaaaatgcgc ccacaccggc
     4681 ctgcagtttt ccggcagctc gggacacgcg ccgaggatgc cgccgctgaa gtcgtttaaa
     4741 cggtggaagt cgctgcccgc gctggcgagc ataccgaagc gttctgccaa aagcgcgtag
     4801 ttgaggcggt cgtttttgca acagtttccg ctgtggactt cgatgcctgc gccgccgagg
     4861 tttttaaatt cttcaaacag attgcgcttg gcggtggcgg acaaatcgta gcgcatcgga
     4921 tgggcaatca ccgccaaccc acccgcgccg ttgacggcgg agacgcagtc ttccagcgtc
     4981 gcccattcgt gtcggacggc gcaggatttg ccgtcgccca agtatttggt gaacgcctgc
     5041 tgcttgtttt tgacgtatcc gtttttgatg aggaactcgg cgacgtgggt gcggctgacc
     5101 atttctttgt ttgccgccaa ctccagcgcg ccgtcgtatg cgccgccgat gcctttcttt
     5161 tcgagcttgt cggcgatggc ttcaagacgt ttcagacggc cttgccgcac ttgcgccaac
     5221 aagttttgca ggttctcgtc ctgctcgtcg aaatccaaac cgacaacgtg tatggtacgc
     5281 ccgcgccacg ttacggagat ttccacgccg ttaatcaggc gcaaaccgag cctgtcggct
     5341 tcggcacgcg cttcggcgat gccgcccgtg tggtcgtggt cggtcaacgc cagcagcgtg
     5401 cagccatttt gatgcgcgag gcgcacgatt tcggcggggg agagcatacc gtcggaaacg
     5461 gtggaatggc agtgcaggtc tatcataagg cgttatgtgc gtggtaaatg gtgggcaggg
     5521 tgtttcaata ttgtcgggcg gtgtggcggc ggtgatttca aaaaacaagt gtatggcaaa
     5581 tgcaaaggaa aagccgctat gccgtcattc ccgcgcaggc gggaatccag actttgattt
     5641 attggaaatg tttaaggtta accgctattt caaacttccg gattcctgct ttcgagggaa
     5701 tggcgatatg accgttttca attttaatct actataaaag actgtctgaa aacgtggttt
     5761 tatcaagccg aactggtttc agacggcatt ttcgtcttcc gcccaacccg cttctttcaa
     5821 cttccgccgt tgcgcgtcgt agcgtgcctt atccgcccag taaatcgtct gaatgcacgc
     5881 ctcgccgcag tcgctgccgc agcattccca cgactcgggt cggacgggtt cgtctaaaag
     5941 cggctcgccc aaaagggctt cggctttatg cttcagggtc atatccatcg gcgatttcca
     6001 agcgcgcacc gtcaaactcg atgacttcgc cgccgcgtat tttggcggtt ttacgggttt
     6061 cggtttcgcc gttgcgcgac accaaccctt cggcaatcaa cgccttcgcc tgtccgccgc
     6121 tttcggcaag tccggccaat ttcaagaggt cgcacagggc gatgtattcg ttgtcttcga
     6181 gatagacggt ggcttccata atgttccctt gcagaaagag gccgttattg tagcacctgc
     6241 cgccgccgta cccaaaatta ccgcaaaacc ggcagcacat cccacaccag ctgttccgta
     6301 aaagtaaaaa tgccgtctga aaccccatat gccgcaatcc gttcaaagaa atcctgccca
     6361 acggcagact gcaaatcctg ttccccgacg aatccgcatt gacgctgatg cacatcctca
     6421 aacgcgaact gcccgataca ccggcaatcg gcatcaaaac gaaatcaaaa acctgtttga
     6481 acgtaaaatt aatttaactg cttgccatcc gtccgaataa ggtatatagt tctttataac
     6541 tagttttata gtcctttata tctatcaata ctccttggga agcctccacc acacggtagg
     6601 aggcattttt ttgccgtcgt aaaagctcaa aaacatttgc aggtcatgcc gtctgaatct
     6661 gaaacggcat gacctacacc gccatctaaa gacaaccctg ctacaatacg ccttttattg
     6721 ttcacgccga ttttgccatg accgagccga cctacatccc cctgcgcctg cataccgagt
     6781 tctcaattac cgacggtatg gtgcggatta aaaaactgat tgccaaagcg caggaatacg
     6841 gtttgcctgc tttgggcatc agcgatttga tgaacgaatt cggtttggtg aaattctata
     6901 aagcctgccg cggcgcaggg attaagccca tcggcgcggc ggacgtgtgg ataggcaatc
     6961 cgaatgcgcc cgacaagccg ttccgcgcta tgctgattat ccgcaacgat gcgggctatc
     7021 tgcgtttgag cgagcttttg acagaggcct atgtcggcaa agaccgcaat atccatcatg
     7081 cggaactcaa ccccgaatgg ctggaaaacg gcgacaacag cggcttgatt tgtttgagcg
     7141 gcgcgcatta cggcgaagtg ggcatgaatc tgttgaacgg caatgaagac gcggcgcgcg
     7201 cggcggcgtt gaaatatgcg gcatggtttc ccgatgcgtt ttatttagag ctgcaacgcc
     7261 tgcccgaacg ccccgaatgg gaggcttgcg tttccggcag cgtgaagctg gcggaggaat
     7321 tgggtttgcc ggtggtggcg acgcatccga cacagtttat gagccgcgac gatttcaacg
     7381 cgcacgaggc gcgagtgtgt atcgcaggcg gctgggtatt gacggacaag aaacgtccgc
     7441 gcgatttcac gccgggccag tttttcattc cgccggaaac catggccgaa cgtttcgccg
     7501 atttgcctga agccttggaa aacacggtag aaattgccaa acgctgcaac ctgcacatca
     7561 cattgggcaa aaacttcctg ccccttttcc ccacgcccga cggtttatca ctcgatgact
     7621 atctcatcaa actctccaac gagggtttgc aggaacgtat ggttcagctt tatcccgacg
     7681 aggcggagcg ggcggcgaaa atgccggaat atcaaaaacg gctggatttt gagctgaaca
     7741 tcattatcca gatgaaattc cccggctatt tccttatcgt acaagacttt atcaactggg
     7801 cgaaaacaca cggctgtccg gtcgggccgg gccgtggttc gggcgcgggt tcgctggtgg
     7861 cgtattcatt gaagattacc gaccttgatc cgctcaaata cgcgctgctg ttcgagcgtt
     7921 tcctaaaccc cgaacgcgtt tctatgcccg acttcgacgt ggacttttgc caaagcaacc
     7981 gcggccgcgt gattgaatat gtgcgcgaga aatacggcgc ggaggcggtc agccagattg
     8041 ttaccttcgg cacgatgtcg tccaaagcgg tcatccgcga cgtcgggcgc gtgttagagc
     8101 tgccgtttat gctgtgcgac aaactgtcca agctgattcc gttggaagcc aacaaacccc
     8161 tgagtttgga aaaagccatg gagaccgagc cgcagatcca ggaattgatc gaagcggaag
     8221 aagcggacga actgattacg ctggcgaaaa agctggaaga tttaacgcgc ggtttgggta
     8281 tgcacgcagg cggcgtgttg attgcgccgg gcaagatttc cgattacagc cccgtgtatc
     8341 aggcggacga atccgcctcg cccgtatcca tgtacgacaa gggcgacgtg gaagatgtgg
     8401 gtttggtgaa attcgacttt ttgggtctgc gcaacctgac cattatcgaa atggcgcagg
     8461 acaacatcaa aaacactacc ggcgacatca tcgatgtcgg caaaatcccg cttgacgacc
     8521 aggtcgccta ccaaatcttc cgcgatgcga acaccaccgc cgtcttccag ttcgagtcga
     8581 ccggcatgaa aaaaatgctg aaaacggcgc acacgaccaa gtttgaagaa ctcatcgcct
     8641 tcgtatcgct ctaccgcccc ggcccgatgg acaacattcc cgacttcgtc gcacgtatga
     8701 aggggcaaga attccaatac atccatccgc tactcgaagg catcctcgcg ccgacctacg
     8761 ggattatggt gtatcaggaa caagtgatgc aggcggcgca aattatcggc ggttactcgc
     8821 tcggcggcgc ggacttgttg cgccgcgcca tgggtaagaa aaagcccgaa gaaatggtga
     8881 aacaccgcga aatcttcgcc gaaggcgcgg caaaacaagg catttcgcgc gaaaaatccg
     8941 acgaaatctt caactacatg gaaaaattcg ccggctacgg tttcaacaaa tcccacgccg
     9001 ccgcctacgc cctgatttcc taccagaccg catggctcaa agcccactac cccgccgaat
     9061 ttatggcggc gaccatgtcg tccgaattgg acaacaccga ccagctcaag catttctacg
     9121 acgactgccg tgccaacggc atcgagttcc tgccgcccga catcaacgaa tccgactacc
     9181 gcttcacgcc gtatccagac atgaaaatcc gctacgcact cggcgcgatt aaaggcacgg
     9241 gcgaagccgc cgtcgaatcc atcatcgccg cgcggcaaag cggcggcaag tttaccggcc
     9301 tcttggactt ctgcgagcgc gtcggcaaag aacacatgaa ccgccgcacc ctcgaggccc
     9361 tgatacgcgg cggcgcgttc gacagcatcg aacccaaccg cgccatgctc ttggcgaaca
     9421 tcgacctcgc tatggacaac gccgaccaaa aagccgccaa cgccaatcag ggcgggcttt
     9481 tcgacatgat ggaagacgcc atcgaaccgg tgcggctcat cgacgcgccg atgtggagcg
     9541 aatcggaaaa actcgccgaa gaaaaaaccg tcatcggctt ttacctgtcc ggccacccgt
     9601 tcggcccgta tgcccaagaa gtccgccaaa tcgccccgca gaaactgtcg aaactcaaac
     9661 cgcaagacag cgtgcgcctc g
//
LOCUS       DAWHSK010000048         9521 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.048, whole genome shotgun sequence.
ACCESSION   DAWHSK010000048 DAWHSK010000000
VERSION     DAWHSK010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9521
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.048"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            336..1154
                     /gene="opc"
                     /locus_tag="WIC03_000784"
                     /gene_synonym="opcA"
     CDS             336..1154
                     /gene="opc"
                     /locus_tag="WIC03_000784"
                     /gene_synonym="opcA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Opc"
                     /protein_id="HEZ3883315.1"
                     /translation="MKKTVFTYAMIALTGTAAAAQELQTANEFTVHTDLSSISSTRAF
                     LKEKHKAAKHIGVRADIPFDASQGIRLEAGFGRSKKNIINLETDENKLGKTKNVKLPT
                     GVPENRIDLYTGYTYTQTLSDSLNFRVGAGLGFESSKDSIKTTKHTLHSSRQSWSAKV
                     HADLLSQLGNGWYINPWSEVKFDLKSRYKLNTGVTSLKKDINQKTNGWGFGLGANIGK
                     KLGESASIEAGPFYKQRTYKESGEFSVTTTSGDVSLTIPKTSIREYGLRVGIKF"
     gene            1317..1961
                     /locus_tag="WIC03_000785"
     CDS             1317..1961
                     /locus_tag="WIC03_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEZ3883316.1"
                     /translation="MLASAIDFILHIDQHLLALSAQYGVWIYAILFLIVFCETGLIVT
                     PLLPGDSLLFAAGGIAALGGMDIHLMVALLSLAAILGDALNFTVGKYFGGRLFANPDS
                     KIFRREYLDKTHRFYEKHGGKTIIIARFMPIVRTFAPFVAGMGKMHYAKFIRYNIIGG
                     LLWVILFSYAGYFFANFPVVKNNLGLVMGGIIIVSVLPGAVEIARAKLAVKSKR"
     gene            1980..3890
                     /locus_tag="WIC03_000786"
     CDS             1980..3890
                     /locus_tag="WIC03_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ3883317.1"
                     /translation="MNILSVENASFAIGHVALLDKTSFQLDSGEKVGLIGRNGAGKSS
                     FLKILAGLQKLDDGQIIVQNNLKIVYVPQESFFDKDATVFDTVAEGLGEIRDLLRRYH
                     HVSHDLEHNSDDVLLKELNELQLEIEAKDGWKLDAAVKQTLGELGLPENEKIGNLSGG
                     QKKRVALAQAWVQKPDVLLLDEPTNHLDIDAIIWLENLLKAFEGSLVVITHDRRFLDN
                     IATRIVELDRGILRSYPGSFSKYSEKKAQELVVEAEHNRLFDKFHAQEEAWIRKGIEA
                     RRTRNEGRVRRLEELRRQRAERRNGQGQVNFKLDSGEKSGKIIAELEHASFAYGDKVI
                     MDKFSAILQRGDKIGLIGPNGIGKTTFLKLILGELQPTYGRIRIGSKQEVAYFDQFRS
                     ALNENDTVFYTLGQGNDYVEVGGKKKHVMSYLEDFLFPPARAQSPVSSLSGGERNRLL
                     LAKLFTRPANILVLDEPTNDLDIDTQELLEDLLRDYQGTVFLVSHDRMFLDNVITQSL
                     VFEGNGRLKEYIGGYQDYIDAKSREAKIQTESAPKTADAEPVKEKPKANRTVKLSYKE
                     QRELDALPDEIAALEAEQAEINAQLSDPEIFKDYEKAGALQSRAEEIEMLLLEKLERW
                     ELLETKQNGNAV"
     gene            3911..4276
                     /locus_tag="WIC03_000787"
     CDS             3911..4276
                     /locus_tag="WIC03_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883318.1"
                     /translation="MFKRPEEIIVLILAVLWIAGTYFLAALFGADAYTVLKITALTLL
                     WSAASFLLWQKNLSRHIWPLLLGCLTACWWPYLNQSVAPGSLPWYASWTFKTILALIP
                     VTAGYLFKWKQYRQRKTKP"
     gene            4387..5862
                     /locus_tag="WIC03_000788"
     CDS             4387..5862
                     /locus_tag="WIC03_000788"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEZ3883319.1"
                     /translation="MISKQEYQAQAAQGYNRIPLVQELLADLDTPLSLYLKLANRPYT
                     YLLESVVGGERFGRYSFIGLPCSHYLKASGKHVDVYQNGEIVEQHDGNPLPFIEAFHN
                     RFKTPEIPSLPRFTGGLVGYFGYETIYNFEHFAHRLKNTTKADPLGTPDILLMLSQEL
                     AVIDNLSGKIHLVVYADPSQPDGYERARERLEDIRTQLRQSCAIPLSLGSKHTEAVSE
                     FGEEPFKACVNKIKDYIFAGDCMQVVPSQRMSMEFTDSPLALYRALRTLNPSPYLFYY
                     DFGDFHIVGSSPEILVRRERNDVIVRPIAGTRLRGKTPAEDLANEQDLLSDAKEIAEH
                     VMLIDLGRNDVGRISKTGEVKVTDKMVIEKYSHVMHIVSNVEGRLKDGMTNMDILAAT
                     FPAGTLSGAPKVRAMEIIEEVEPSKRGIYGGAVGVWGFNNDMDLAIAIRTAVVKNNTL
                     YVQSGAGVVADSDPASEWQETQNKARAVIHAAQMVQEGLDK"
     gene            5859..6405
                     /locus_tag="WIC03_000789"
                     /pseudo
     CDS             5859..6405
                     /locus_tag="WIC03_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242626.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
     gene            6402..7538
                     /locus_tag="WIC03_000790"
     CDS             6402..7538
                     /locus_tag="WIC03_000790"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEZ3883320.1"
                     /translation="MKNISLSPPATLGILGGGQLGRMFTVAAKTMGYKVTVLDPDPNA
                     PAAEFADRHLCAPFNDQTALDELAKCAAVTTEFENVNADAMRFLAKHTNVSPSGDCVA
                     IAQNRIQEKAWIRKAGLQTAPYQAVCKAEDITEASAQFLPGILKTATLGYDGKGQIRV
                     KTVDELKAAFAEHGGVDCVLEKMVDLRGEISVIVCRLNNDNVQTFDPAENIHENGILA
                     YSIVPARLSADIQQQARQMAQRLADELNYVGVLAVEMFVVGDTHELVVNEIAPRPHNS
                     GHHTVDACAADQFQQQVRLMCNLPPADTKLLSSCCMANILGDVWQEDGGEPDWFPLQS
                     RPDAHLHLYGKKTAHKGRKMGHFTVLTTDSDTAFQEAKKLHQSL"
     gene            7585..8067
                     /locus_tag="WIC03_000791"
     CDS             7585..8067
                     /locus_tag="WIC03_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ3883321.1"
                     /translation="MNLLTLLRPATVQDCKDIFKVHLHSVQYTCILSYNEHALKVWEG
                     LLNTESYLPTISDPDKALWVAEYKGNIQGFFQIDCQEAQLDALYVHPLFHNLGLGTAL
                     LHQAETIAHKSGLSFLKLYASLNSVPFYLLNRYESLGSAVLQLDPSIKIKCELMRKHL
                     "
     gene            8173..9228
                     /locus_tag="WIC03_000792"
     CDS             8173..9228
                     /locus_tag="WIC03_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ3883322.1"
                     /translation="MKTYAPALYTAALLTACSPAADSNHPSGQNAPANTESDGKNITL
                     LNASYDVARDFYKEYNPLFIKTYQSEHPGTSVSIQQSHGGSSKQALSVANGLQADVVT
                     MNQSSDIDLLEKKGLVEKGWQQALPDHAAPYTSTMVFLVRKNNPKQIRDWNDLAKDGV
                     NIVIANPKTSGNGRYAFLGAYGYGLKATNGNEQEAQKLVASILKNTPVFENGGRAATT
                     TFTQRNIGDVLITFENEANYVSKKLTQGQFEIVYPSYTISAESPVAVVNSVVAKKGTQ
                     KTARAYLEYLWSEPAQELAASLYLRPRNPEVLARHKADFPDLDTFSPEEKFGGWDNIM
                     KTYFADGGIFDRLTAQK"
     gene            9225..9422
                     /locus_tag="WIC03_000793"
     CDS             9225..9422
                     /locus_tag="WIC03_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883323.1"
                     /translation="MTAMPSEPPFRRHSTHQNRKTAPKIIITRHNGKPDTKPNTAAKM
                     PSEAVGATILIWGITFLNHPF"
BASE COUNT         2649 a         2704 c         2098 g         2070 t
ORIGIN      
        1 attcccgcct gcgcgggaat gacgatgaaa agattgttgt cgcttcggat aaatttttac
       61 cgtgttgggt tctagattcc cgcctgagcg ggaatgacgg cggcgggttt ctgtttttcc
      121 gataaataca cacaaactaa aatttcgtca ttcccataaa aaacagaaaa ccaagtgaga
      181 ataacaattc gttgtaaaca aacaactatt tgttaatttt tgttaatgta tgcaaaatct
      241 cgcccctgaa agtttaagaa tataattgta gcgtaacgat tatttacgtt atgttaatat
      301 atccgactac aattcaaatt ttagaggttt taactatgaa aaaaacagtt tttacatatg
      361 ccatgatcgc cctgaccggt actgccgccg ctgcacaaga gcttcaaacc gctaatgagt
      421 ttaccgtcca caccgacctc tcttccattt cttcaactcg tgctttcctg aaagaaaaac
      481 acaaagctgc caaacacatc ggcgtacgtg ctgatattcc ttttgatgcc agccaaggca
      541 tccgcttgga agccggtttc gggcgcagca aaaaaaatat tattaatttg gaaacagatg
      601 agaacaagct gggtaagact aaaaatgtaa aactgcccac cggcgttcct gaaaaccgta
      661 tcgatcttta cacaggctac acctataccc aaacgttaag tgattcttta aatttccgtg
      721 tgggtgccgg cttgggtttt gaatcttcaa aagacagcat taaaaccacc aagcatacgc
      781 ttcacagcag ccgtcagtcg tggtcagcca aagttcacgc ggatttgctt tcccaactgg
      841 gtaacggctg gtatatcaac ccttggtctg aagtgaaatt tgacctcaag tcccgctata
      901 aattaaacac cggcgttacc agtctcaaaa aagacatcaa tcaaaaaacc aacggctggg
      961 gctttggatt gggtgcaaat attggtaaaa aactgggcga atccgccagc atcgaggcgg
     1021 ggccgttcta caaacaacgc acttacaaag aatccggcga gtttagtgta acaaccacga
     1081 gtggcgacgt atcgctcacc atcccgaaaa ccagtattcg tgaatacggc ttgcgcgtcg
     1141 gcataaaatt ctgatgattt gaaatcatca ttgtcgcttt aaatgccaaa ccgcaaacta
     1201 ttttggtttg cggtttttac gtgaaatgaa tttgaatagc cgatgccgtc tgaaaatttc
     1261 agacggcatt tttccaatcg gttaaaatac gcggtttgtt tttttaggaa cgcgccgtgc
     1321 ttgcctccgc catcgacttc atcctccata tcgaccaaca cctgctcgcg ctgtcggcgc
     1381 aatacggtgt gtggatttat gcgattctgt ttttgattgt tttttgcgaa accggcctga
     1441 ttgttacgcc cttactgccc ggcgattcgc tgctgtttgc cgccggcggg attgccgcgc
     1501 tgggcggtat ggatattcat ttgatggttg cattattaag ccttgccgcc atactcggcg
     1561 acgcgctcaa ttttaccgtc ggcaaatatt tcggcggcag gctgttcgcc aaccccgatt
     1621 ctaaaatctt ccgacgtgaa tatctcgata aaacccaccg tttttacgaa aaacacggcg
     1681 gcaaaaccat catcattgcc cgttttatgc ccatcgtccg caccttcgcc cccttcgtcg
     1741 ccggtatggg gaaaatgcac tatgccaaat ttatccgcta caacatcatc ggtggcttgt
     1801 tgtgggttat cctgttttct tacgcgggct atttctttgc caacttcccc gtcgtaaaaa
     1861 acaatctggg cttggtgatg ggcggcatca ttattgtttc cgtcctgccg ggtgcggttg
     1921 aaattgcccg cgccaaactt gcggtaaaat ccaaacggta actgttgaga atttgtatca
     1981 tgaatatatt atctgtagaa aacgcttctt ttgccatcgg ccacgttgcc ttgctcgaca
     2041 aaacttcttt tcaactcgac agcggcgaaa aagtcggctt aatcggacgc aacggcgcag
     2101 gcaagtcttc gtttttaaaa atcctcgccg gtttgcaaaa gctcgacgac gggcagatta
     2161 ttgttcaaaa caacctcaaa atcgtttatg taccgcagga atcctttttt gataaggacg
     2221 caaccgtatt tgataccgtt gccgaaggtt tgggcgaaat tcgcgattta ttgcgccgtt
     2281 atcatcatgt cagccatgat ttggagcaca attcagacga cgttttatta aaagaactca
     2341 acgaattaca actcgaaatc gaagcgaagg acggctggaa gctggatgcg gcagtcaagc
     2401 agactttggg cgaactcggt ttgccggaaa acgaaaaaat cggcaacctt tccggcggtc
     2461 agaaaaagcg tgtcgccttg gcgcaggctt gggtgcagaa gcccgacgta ttgctgctgg
     2521 acgaaccgac caaccatttg gatattgacg cgattatctg gttggaaaac ctgctcaagg
     2581 cgtttgaagg cagcttggtc gtgattaccc acgatcgccg ttttttggac aatatcgcca
     2641 cgcggattgt cgaactcgat cgcggtattt tgcgttccta tcccggctcg ttctccaaat
     2701 atagcgagaa aaaagcgcaa gagttggtag tcgaagcgga acacaaccgc ctcttcgaca
     2761 aattccacgc gcaggaagaa gcgtggatac gcaaaggcat cgaagcgcgc cgtacccgca
     2821 acgaaggccg cgtgcgccgt ttggaagaac tgcgccgcca gcgtgccgaa cgccgcaacg
     2881 gacaagggca ggtcaatttc aagctcgaca gcggcgagaa aagcggcaaa atcatcgccg
     2941 agctggaaca cgcctcgttt gcttatggcg acaaagtcat tatggacaaa ttctccgcca
     3001 tcttgcagcg cggcgacaaa atcggcttaa tcggtccaaa cggcatcggc aaaaccacat
     3061 ttttaaaact gattttgggc gaattgcagc cgacctacgg cagaatccgt atcggcagca
     3121 agcaggaagt cgcctatttc gaccagttcc gcagcgcgtt gaatgaaaac gacaccgtgt
     3181 tttacacgct cggacagggc aatgattacg ttgaagtcgg cggtaagaaa aagcatgtga
     3241 tgagctattt ggaagatttc ctgttccccc ctgcccgcgc gcaaagtcct gtttcttcac
     3301 tctccggcgg cgaacgcaac cgccttttgc tggcaaaact ctttacccgt cccgccaata
     3361 ttttggtctt ggacgaaccg accaatgact tggacatcga cacccaagag ctgctcgaag
     3421 acctgttgcg cgattatcaa ggcacggtat tccttgtctc gcacgaccgt atgttcctcg
     3481 ataacgtgat tacccaaagc cttgtttttg aaggaaatgg aaggttaaaa gaatatatcg
     3541 gcggctatca ggactacatc gacgcaaaat cacgcgaagc gaagattcag acggaatccg
     3601 cacccaaaac agccgatgcc gaaccggtga aagaaaaacc caaagccaac cgcacggtca
     3661 aactgtccta caaagaacag cgagaactcg atgccctgcc cgacgaaatc gccgccttgg
     3721 aagccgaaca ggcagaaatc aacgcgcagc tttccgatcc cgaaatcttc aaagattacg
     3781 aaaaagccgg cgcactgcaa agccgcgccg aagaaatcga aatgctgctt ttggaaaagc
     3841 tggaacgctg ggagctgctg gaaaccaaac agaacggcaa tgccgtctga aatcaaacag
     3901 gaagaaaaaa atgttcaaac gacctgaaga aatcatcgtc ctgatacttg ccgtcctctg
     3961 gattgccggt acgtattttc tcgccgccct gtttggagcg gacgcttata ccgtgttaaa
     4021 aatcactgcc cttaccctgc tttggtctgc cgcatcattc ctcctttggc aaaaaaacct
     4081 cagccggcat atctggccgc tgctgctcgg ctgcctgact gcctgttggt ggccgtatct
     4141 gaatcaatcg gtcgcacccg gttctttacc ttggtatgca tcatggacgt tcaaaaccat
     4201 cttagccctg attcccgtaa ccgcaggtta tctgtttaaa tggaaacaat accggcagcg
     4261 caaaacaaag ccgtaagccc gtctttttca gacggcatct ccggtcagac tgtcaacaat
     4321 tccgataaaa tgctatactc gcgcccgttg ccgcgccgtc cgaaaccgcc ctttgggaaa
     4381 gcagaaatga tcagcaaaca agaataccaa gcccaagccg cccaaggcta caaccgcatc
     4441 ccgctcgtgc aagaactcct tgccgatttg gatacgccgc tttccctcta tctcaaactc
     4501 gccaaccgcc cctataccta cctgctcgaa tccgttgtcg gtggcgaacg tttcggccgc
     4561 tattctttta tcggcctgcc ttgcagccac tatctcaaag ccagcggcaa acacgtcgat
     4621 gtttatcaaa acggcgaaat tgtcgagcaa cacgacggca accccctgcc ctttatcgaa
     4681 gccttccaca accgcttcaa aacacccgaa atcccaagcc tgccgcgctt taccggcgga
     4741 ctggtcggct acttcggtta cgaaaccatc tacaattttg aacacttcgc ccaccgcctg
     4801 aaaaacacca ccaaagccga cccgctcggc acgcccgaca tcttgctgat gctgtcgcaa
     4861 gaattggcgg tgatcgacaa tttgagcggc aaaatccatc tcgtcgttta cgccgatccg
     4921 tcgcagcccg acggctacga acgcgcccgc gaacgcctcg aagacatccg cacccagttg
     4981 cgccaaagct gcgccatccc gctctcgctc ggcagcaaac acaccgaagc cgtcagcgaa
     5041 ttcggcgaag agccgttcaa agcctgcgtt aacaaaatca aagactacat cttcgcaggc
     5101 gactgcatgc aggttgtccc cagccagcgc atgagcatgg aattcaccga cagcccgctc
     5161 gccctctacc gcgccctgcg tacgctcaac ccttcgcctt atctctttta ctacgatttc
     5221 ggcgatttcc acatcgtcgg ctcctcgccc gaaatcctcg tccgacgcga acgcaacgac
     5281 gttatcgtcc gccccatcgc cggcacgcgc ctgcgcggca aaacccccgc cgaagacctt
     5341 gccaacgaac aagatttgtt aagcgatgcc aaagaaatcg ccgaacacgt catgttgatt
     5401 gacttaggac gcaacgacgt cggccgcatc agtaaaaccg gcgaagtcaa agttaccgac
     5461 aaaatggtga ttgaaaaata ctcccatgta atgcacatcg tttccaacgt cgaaggccgt
     5521 ctgaaagacg gcatgaccaa catggacatc ctcgccgcta ccttccctgc cggcacactc
     5581 tccggcgcac ccaaagtccg cgccatggaa atcatcgaag aagtcgaacc gagcaaacgc
     5641 ggcatctacg gcggtgccgt cggcgtatgg ggtttcaaca acgacatgga tttggcaatc
     5701 gccatccgca ccgccgtagt aaaaaacaac acgctatacg tccaaagcgg cgcgggcgtg
     5761 gttgccgact ccgatccggc ctccgaatgg caggaaacac aaaacaaagc ccgagccgtc
     5821 atacacgccg cgcaaatggt acaggaaggg ttggataaat gacttatgaa aaactttatg
     5881 agcttcgcca aacactcaga ccgactacca aagacggtct ctatacagat aatgaaaaaa
     5941 atcgagaaat cctaacggtt agatggagcg gcaacacaga aaatcctaaa aatttctcag
     6001 ctgtcgctat aggaattaat ccaagtaaag caaatgatga aagaagcgac aaaaccctta
     6061 cccaacttgc acgtttcttg gatatgtacg gttttaccaa cttcaaaatg ttaaatattt
     6121 tttccagtta ctctacacag caaacaggaa ttaggacaaa cacccaaacc gatttttcca
     6181 aatttaaagg atgtttggaa gatgctgata tgattattct tgcatggggt acagaccgcg
     6241 gtgcttataa ggatgaaaaa aaacagaata ctggaatttc taaaagcaga aaaatttatg
     6301 gaaaaagtat tctgtatttc tgaaactggt aattccagcg atacaagaca tccgtcaaga
     6361 attagttata gctatcaact tgtacaattt gaagagtcag catgaaaaac atatctcttt
     6421 ctccgcccgc cacgctcgga atcctcggcg gcggacaatt aggcagaatg tttaccgttg
     6481 ccgccaaaac catgggctac aaagtaaccg tactcgaccc cgatccgaac gctccggcgg
     6541 cggaatttgc cgaccgccat ttgtgcgcgc cgtttaacga ccagaccgcg ttggacgagt
     6601 tggcaaaatg cgcggcggtt acgaccgaat ttgaaaacgt caatgccgac gcgatgcgtt
     6661 ttctggcaaa acataccaac gtttccccca gcggcgactg cgttgccatc gcgcaaaacc
     6721 gcattcagga aaaggcatgg atacgcaaag caggtctgca aaccgcgccg tatcaagcag
     6781 tttgcaaagc cgaagacatc actgaagcaa gcgcgcaatt cctgcccggt attctgaaaa
     6841 cggccacttt gggctacgac ggcaaaggtc aaatccgcgt caaaacggtg gacgaactca
     6901 aagccgcgtt tgccgaacac ggcggcgtgg attgcgtttt ggaaaaaatg gtggatttgc
     6961 gcggcgaaat ttccgttatc gtatgccgtc tgaacaatga caacgtgcaa actttcgatc
     7021 ctgccgaaaa cattcacgaa aacggtatcc tcgcctactc catcgtccca gcccgactga
     7081 gtgccgacat tcagcaacag gcgcgacaaa tggcgcagcg tttggccgat gaattgaact
     7141 acgtcggcgt attggcggta gaaatgtttg ttgtcggcga cacgcatgaa ttggtcgtca
     7201 acgaaatcgc gccgcgtccg cacaattccg gccaccatac cgtcgacgcc tgcgcggcag
     7261 accaattcca gcaacaggtc cgcctgatgt gcaacctgcc acctgctgac accaaattgc
     7321 tgagttcttg ctgtatggcg aatattttgg gcgacgtttg gcaggaagac ggcggcgaac
     7381 cggattggtt tcccctgcaa agccggccgg acgcgcacct gcacctttac ggcaaaaaaa
     7441 ccgcgcacaa agggcggaaa atgggacact ttaccgtttt aaccaccgat tcggacaccg
     7501 catttcaaga agcaaaaaaa ctgcatcagt ccctataatc cgaccaacgg cacagatgcc
     7561 gtctgaagcc gccgacatac tgccatgaac ctgctgacac tgttacgccc cgccaccgtg
     7621 caagactgca aagacatctt caaagtccac ctgcactccg tccaatacac ctgcatcctc
     7681 agctacaacg aacacgccct aaaagtatgg gaaggcttgt taaacacaga aagctacctg
     7741 ccgaccattt ccgatcccga caaagcactt tgggttgccg aatacaaagg caacatacag
     7801 ggatttttcc aaatcgactg ccaagaagca caactcgatg cactttacgt ccaccccctg
     7861 ttccacaacc tcggactggg cactgccctg cttcatcagg cggaaaccat cgcccacaaa
     7921 tctggtttga gctttctgaa actctacgca tcgctcaact ccgtcccgtt ttaccttctc
     7981 aaccgctacg aatcgctcgg ttcggcagta ttgcagcttg accccagcat caaaatcaaa
     8041 tgcgaactga tgcggaaaca cctctgatcc gtccttataa ccaaaactga taatcttcaa
     8101 atcaaacatt atttccacaa gcgcagacag ccgtataaag ttgccccatc taccaaccga
     8161 caggacaagc atatgaaaac ctacgcaccg gcactctata ccgcagccct gctcaccgcc
     8221 tgcagccccg cagccgattc aaaccatccg tccggacaaa atgccccggc caataccgaa
     8281 tccgacggaa aaaacattac cctgctcaac gcctcatacg atgtggcacg ggatttttac
     8341 aaagaataca accccttatt tatcaaaaca taccaatccg aacaccccgg cacatccgtc
     8401 agcatccaac agtcccacgg cggctccagc aaacaggcat tatccgtagc caacggcctt
     8461 caagccgatg tcgtaaccat gaaccaatcc tccgacatcg acctgctcga aaaaaaagga
     8521 ctggtagaaa aaggctggca acaagccctc cccgaccacg ccgcgcccta caccagcact
     8581 atggttttcc ttgtccgaaa aaacaacccc aaacagatcc gcgattggaa cgaccttgcc
     8641 aaagacggcg ttaacatcgt catcgccaat cccaaaacct cgggcaacgg acgctacgcc
     8701 ttcctcggcg catacggtta cggtctgaaa gccaccaacg gcaacgaaca ggaagcccaa
     8761 aaactcgtcg catccatcct caaaaacacc cccgtttttg aaaacggcgg acgcgccgcc
     8821 accaccacct tcacacaacg caacatcggc gacgtactca ttacttttga aaacgaagcc
     8881 aactacgtca gcaaaaaact gacccaagga cagtttgaaa tcgtctatcc gagctacacc
     8941 atttccgccg aaagccccgt cgccgtcgtc aacagcgtcg tcgcgaaaaa aggcacgcaa
     9001 aaaaccgccc gcgcctatct cgaatacctt tggagcgaac cggcgcaaga actcgccgcc
     9061 tcactctacc tgcgcccccg caaccccgaa gtattggcaa gacacaaagc cgacttccca
     9121 gacttagaca ccttctctcc cgaagaaaaa ttcggcggat gggacaacat catgaaaacc
     9181 tacttcgctg acggcggcat attcgaccgc ctgaccgcac aaaaatgacc gctatgccgt
     9241 ctgaaccgcc tttcagacgg cattccacac atcaaaacag aaaaaccgcg cccaaaatca
     9301 tcatcacaag acacaacggc aaaccggata caaaacccaa caccgccgcc aaaatgccgt
     9361 ctgaagccgt cggcgcaacc atccttatct ggggcataac tttccttaac catccctttt
     9421 gacagccaaa taaacagtgc ttttgcctgt cagaaacaaa accaatattt tgcagttcgt
     9481 atcggaacat ttatagtgga ttaacaaaaa ccagtacagc g
//
LOCUS       DAWHSK010000049         9498 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.049, whole genome shotgun sequence.
ACCESSION   DAWHSK010000049 DAWHSK010000000
VERSION     DAWHSK010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9498
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.049"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            123..2150
                     /gene="uvrB"
                     /locus_tag="WIC03_000794"
     CDS             123..2150
                     /gene="uvrB"
                     /locus_tag="WIC03_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEZ3883324.1"
                     /translation="MEVIRYPDSPFKLHQPFPPAGDQPTAIADLLEGLSDGLAYQTLL
                     GVTGSGKTYTMANVIAQSGRPAIIMAHNKTLAAQLYAEMREFFPENAVEYFVSYYDYY
                     QPEAYVPSRDLFIEKDSAINEHIEQMRLSATKNLMTRDDVIIVATVSAIYGIGDPTEY
                     QQMVLSVKEGDTIEQRDIIATLVSMQYERGDLDFKRGSFRVRGDVIDVYPAESSENAL
                     RISLFDDEIDRLDMFDPLSGSLHQRVGRYTVFPSSHYVTPRDTVLRACESIKEELRER
                     IDFFTKENRPVEQQRIEQRTRFDLEMLYEMGFCKGIENYSRHFSGKKEGEPPPTLMDY
                     LPDNAIMFIDESHVTVTQIGGMYKGDASRKQNLVDYGFRLPSARDNRPLKFHEFEKVM
                     PQTVFVSATPAKYEEEHAGQVVEQVVRPTGLVDPQIIIRPVAPQVDDLMSEINDRIQK
                     GERVLVTTLTKRMAEQLTDYYSELGIKVRYLHSDIDTVERVEIIRDLRLGLFDVLVGI
                     NLLREGLDIPEVSLVAILDADKEGFLRSHRSLIQTIGRAARNVNGVAILYADKITDSM
                     KAAIDETERRREKQIKFNEEHGIVPQQIKKQVKDIIDGVYHEEDSGKGRRQGKNKVKV
                     GEIHNEEDAIKEIAKLEKAMQQAARDLQFEEAAVLRDRIRNIKESLLFGAE"
     gene            2186..3070
                     /locus_tag="WIC03_000795"
     CDS             2186..3070
                     /locus_tag="WIC03_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883325.1"
                     /translation="MTSIILELQSLAANPNSDVEELVSKTLMVARKLKINELTEWCNN
                     ELQGYSSKNLPKYRIFYGELKAFNPYRGDVPFMLPDELRERVTRVEYTSSISEIRNLL
                     KTKNNNFIRILSSKEKQFLMNLQDTPFPLEPKIIIYPINLMNIQSKVRQLILAWALRL
                     EEEGILGDGIQFTKSKQEKAMSNQFNIQNMQGFIGDIFGGNVQQNIQDGIHIEKENFN
                     KLANFLAKNGIPSAELEDLRNALIEDGQPTDKKFGSKVSAWIGNIITKASTGAIDVSV
                     GTIAGILTNAISKYYGLM"
     gene            3082..3300
                     /locus_tag="WIC03_000796"
     CDS             3082..3300
                     /locus_tag="WIC03_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883326.1"
                     /translation="MCGSTTHAVPNRPKPRACLRLTPYHPTNTASVGWILVSDKTFNI
                     PIIAIHCSNAQNVGFENPTYKIIPSIIL"
     gene            3297..3788
                     /locus_tag="WIC03_000797"
     CDS             3297..3788
                     /locus_tag="WIC03_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ3883327.1"
                     /translation="MKYRRFYRNGGTYFFTVVANKRQKILTDDAVRLALRQAVMAVRE
                     RYPFEILAWVLMPDHLHTIWRLPDNDSAYSERWRQIKRHSKYLIGGNLRLWQKRFWEH
                     TIRDEADFACHFDYLHFNPVKHGYVGQISDWRFSTFHRYVKQGIYPHNWGGGNADFSI
                     EYD"
     gene            3860..4021
                     /locus_tag="WIC03_000798"
     CDS             3860..4021
                     /locus_tag="WIC03_000798"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883328.1"
                     /translation="MRWLNLKNSTSVHNTQGIRTKEAVEQGLRGNNRPEKGIGNRWNY
                     DGFIKELAK"
     gene            4071..4355
                     /locus_tag="WIC03_000799"
     CDS             4071..4355
                     /locus_tag="WIC03_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883329.1"
                     /translation="MKVITITLFTSCILSGCYGLEYGMSGGIPHDDWARGSHVSQAKA
                     DAAWKECSDKYMRNGGSGLVEQCMNDKGLIRLSEIRRRQKRAETQRAVIK"
     gene            complement(4480..5196)
                     /gene="trmB"
                     /locus_tag="WIC03_000800"
     CDS             complement(4480..5196)
                     /gene="trmB"
                     /locus_tag="WIC03_000800"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEZ3883330.1"
                     /translation="MTDTAENQTQNNWQAEHPRSIRSFVLRQSHMTSAQQRAIDTLWG
                     SFGIDYQATPADLDARFGSSRPKILEIGFGMGTATAEIARRLPETDFLAIDVHGPGVG
                     NLLKLIEENHLENIRVMRHDAVEVVENMLQDGSLDGIHIFFPDPWHKKRHHKRRLIQA
                     PFIAKLLPKLKTGGYIHLATDWEEYAQQMLEVLSSFDSLQNTAADYAPTPDYRPETKF
                     EARGKRLGHGVWDLVFKRIG"
     gene            complement(5318..6721)
                     /locus_tag="WIC03_000801"
     CDS             complement(5318..6721)
                     /locus_tag="WIC03_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HEZ3883331.1"
                     /translation="MNNPDLLYHQALERLSQEQYDFTEVRRLLTEAASAGHPAAAFEL
                     AKHLMDADSPHQDREQGMEMLRIAAEQGHPYARYNLAYIQELEGARPETLIPLYRPLA
                     EEGLPEAQVRLMYLLYASRHFEEALKWAKTTAKNNNPHGQYLLAQYCQYGTPPDFETA
                     HLLYREAAAQGLAAAQWQLGLQYRFGQGTPTDTTQAINHLRAAAQQGYIPAYTPLAEL
                     ILPTAPDEAVYRFQQAAQENDPDAHAALADIYLQGKHLERNHKLALHHAEAAAAERHP
                     EGLRILGDICRYSLGIAPDTEKARHYYRQAAEAGSLSAYQKLISDSALNHPNQYGGIK
                     DSAIRRQRAERLYQKAQALHYGLQCAPEYAAALKLYTEAAELGHSKAQTNLGSMYYFG
                     QGMTADYNEARKWFEKAAAKKDSMAFYNLACIHYSGHGVGPDNEKACRYLQEAINSGH
                     EQKSFLQELLQQWQNAV"
     gene            complement(6840..8726)
                     /gene="uvrC"
                     /locus_tag="WIC03_000802"
     CDS             complement(6840..8726)
                     /gene="uvrC"
                     /locus_tag="WIC03_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEZ3883332.1"
                     /translation="MPSETVFQVIDMNKETRFPEHFDIPLFLKNLPNLPGVYRFFNES
                     GNVLYVGKAVNLKRRVSGYFQKNDHSPRIALMVKQVHHIETTITRSESEALILENNFI
                     KALSPKYNILFRDDKSYPYLMLSGHQYPQMAYYRGTLKKPNQYFGPYPNSNAVRDSIQ
                     VLQKVFMLRTCEDSVFEHRDRPCLLYQIKRCSAPCVGHISEEDYHDSVREASTFLNGK
                     TDELTRTLQHKMQTAAANLQFEEAARYRDQIQALGIMQSNQFIDSKNPNNPNDIDLLA
                     LAVSDGIVCVHWVSIRGGRHVGDKSFFPDTKNDPEPNGQDYAEAFVAQHYLGKSKPDI
                     IISNFPMPDALKEALESEHGKQMQFVTKTIGERKVWLKMAEQNAQMAIAQRRLQQSSQ
                     QHRIDELAKILGMDSDGLNRLECFDISHTQGEATIASCVVYDEQNIQPSQYRRYNITT
                     AKPGDDYAAMREVLMRRYGKMQEAEANGETVKWPDVVLIDGGKGQIGVAVSVWEELGL
                     HIPLVGIAKGPERKAGMEELILPFTGEVFRLPPNSPALHLLQTVRDESHRFAITGHRK
                     KRDKARVTSSLSDIPGVGSKRRQALLTRFGGLRGVVAASKEDLEQVEGISKALAETIY
                     EHLH"
     gene            complement(8790..>9498)
                     /locus_tag="WIC03_000803"
     CDS             complement(8790..>9498)
                     /locus_tag="WIC03_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEZ3883333.1"
                     /translation="VVGAGIAAEVEDVGLVKAGKAEFAELTLPEFSDGVWDIASIVAV
                     SVDNKPIGAFALADALKADTAEAIGRLKKHNIDVYIMSGDNQGTVEYVAKQLGIAHAF
                     GNMSPRDKAAEVQKLKAAGKTVAMVGDGINDAPALAAANVSFAMKGGADVAEHTASAT
                     LMQHSVNQLADALLVSQATLKNIKQNLFFAFFYNILGIPLAALGFLNPVIAGAAMAAS
                     SVSVLSNALRLKRVKID"
BASE COUNT         2278 a         2234 c         2533 g         2453 t
ORIGIN      
        1 tgggtagcga ttgggtttca gacggcatac attcaggatt tcaccgcctt atgccgtctg
       61 aaagacgttt ccgacccatc cctactacac gctttgtccg caaacgcatc acaggcaaga
      121 ttatggaagt cattcgatac cccgattccc ccttcaaact ccaccaaccc ttcccgcccg
      181 caggcgacca gcctaccgct attgccgact tgctcgaagg gctttcagac ggccttgcct
      241 accaaaccct gctcggcgta accggttcgg gcaaaaccta caccatggcg aacgtcatcg
      301 cccaaagcgg ccgacccgcc atcatcatgg cgcacaacaa aacccttgcc gcccagcttt
      361 atgccgaaat gcgcgagttt ttccctgaaa acgcggtgga atatttcgtc tcctactacg
      421 actattacca acccgaagcc tatgtgccaa gccgcgattt gttcatcgaa aaagacagtg
      481 cgatcaacga acacattgaa caaatgcgcc tttctgccac caaaaacctg atgacgcgcg
      541 atgacgtgat tatcgttgcc actgtgtccg ccatttacgg tatcggcgac ccgactgagt
      601 atcaacaaat ggtgttgtcc gtcaaagaag gcgacaccat cgagcagcgc gacatcatcg
      661 ccacgctcgt ttccatgcag tacgaacgcg gcgatttgga tttcaaacgc ggcagcttcc
      721 gcgtgcgcgg cgacgtgatt gacgtgtacc ccgccgaaag ctccgaaaac gccttgcgta
      781 tcagcctgtt tgacgacgaa atcgaccgcc tcgatatgtt tgacccgctt tcaggcagcc
      841 tgcaccaacg cgtcggccgc tacaccgtct tcccgtccag ccactacgtt accccgcgcg
      901 acaccgtatt gcgcgcctgc gagtccatca aagaagaatt gcgcgaacgc attgatttct
      961 ttaccaaaga aaaccgtccc gtcgagcaac aacgcatcga acagcgcacc cgtttcgacc
     1021 tcgaaatgct ctacgaaatg ggcttctgca aaggtatcga aaactactcc cgtcacttct
     1081 ccggcaaaaa agaaggcgaa ccgccgccca cgctgatgga ctacctgccc gacaacgcca
     1141 tcatgttcat cgacgaaagc cacgttaccg tcacacaaat cggcggcatg tacaaaggcg
     1201 acgcatcgcg caagcaaaac ctcgtcgatt acggcttccg cctaccttcc gcccgcgaca
     1261 accgcccgct caaattccac gaatttgaaa aagtcatgcc gcaaaccgtc ttcgtttccg
     1321 ccacccccgc caaatacgaa gaagaacacg ccggacaagt ggtcgaacaa gtcgtccgcc
     1381 ccacagggct ggtcgaccct caaatcatca tccgccccgt cgccccccaa gttgatgact
     1441 taatgagcga aatcaacgac cgcatccaaa aaggcgaacg cgtactcgtt accaccctta
     1501 ccaaacgcat ggcggagcaa ctcaccgact attacagcga actcggcatc aaagtgcgct
     1561 acctgcacag cgacatcgac accgtagagc gcgttgaaat tattagagat ttacggctcg
     1621 gattgtttga cgtactcgtc ggcatcaatc tcttgcgcga aggcctcgac atcccagaag
     1681 tctccctcgt cgccatcctc gacgcagaca aagaaggctt cctgcgctcc caccgcagcc
     1741 tgattcaaac cataggccgc gctgcgcgca acgtgaacgg cgtcgcgatt ctgtacgccg
     1801 acaaaatcac cgactctatg aaagccgcca tcgacgaaac cgaacgccgc cgtgaaaaac
     1861 aaattaaatt caacgaagaa cacggcatcg tgccgcaaca gattaaaaaa caggtcaaag
     1921 acatcattga cggcgtgtac cacgaagaag acagcggcaa aggccgtaga caaggcaaaa
     1981 acaaagttaa agtcggcgaa attcacaacg aagaagacgc gattaaagaa atcgccaaac
     2041 tcgaaaaagc catgcaacag gcggctaggg atttgcagtt tgaagaagcg gctgttcttc
     2101 gtgataggat acggaatatt aaagagagtt tgttgtttgg ggcggaataa ttttattatt
     2161 tttatttaac atggagatta attaaatgac ttctattatt ttagaactcc aaagcctagc
     2221 tgccaatcca aatagtgatg tagaagagtt agtgagtaaa acattaatgg ttgcaagaaa
     2281 attaaaaatt aatgaattaa ctgaatggtg taataatgaa ttacaaggtt attcatcaaa
     2341 aaatttacca aaatatagaa tattttatgg agagttaaaa gcttttaacc cttatcgtgg
     2401 cgatgttcca tttatgcttc ccgatgaatt gagagaacga gtaacaagag ttgaatatac
     2461 ctcatcaatt agcgaaataa gaaacttact aaaaacaaaa aataataatt ttatacgaat
     2521 tctatctagt aaagaaaaac agtttttaat gaatttacaa gatactccat ttccacttga
     2581 acctaaaata attatttatc caattaactt aatgaatatt caatcaaaag taagacaact
     2641 aattttagct tgggcattac gactggaaga ggaaggcatt ttaggagacg gtatccaatt
     2701 tacaaaatca aaacaggaga aagcaatgtc aaaccaattc aatattcaga atatgcaagg
     2761 gtttattggg gatatttttg gcggtaatgt tcagcaaaat attcaagacg gtattcatat
     2821 tgaaaaagaa aatttcaata aactggctaa ctttttagca aaaaacggga ttccatctgc
     2881 cgaattagaa gatttacgaa atgcactgat agaagatggt cagcccacag ataaaaaatt
     2941 cggctcaaaa gtaagtgcat ggattggcaa tattattaca aaggcttcta ccggtgcaat
     3001 tgatgtttca gtaggtacta ttgcaggaat attaactaat gcgatttcaa aatattatgg
     3061 cttaatgtaa gcaaatgcaa tatgtgcggc tccaccacac acgccgtccc caaccgccca
     3121 aaaccgcgtg cctgcctgcg gcttaccccc taccacccaa ccaacacagc aagcgtaggt
     3181 tggatacttg tatccgacaa aacctttaac attcccatca ttgcaatcca ttgtagcaat
     3241 gcccaaaatg tcggattcga gaatccgacc tacaaaatca ttccgagcat aatactatga
     3301 aataccgtcg tttttaccgc aatggcggca cttacttttt tacggttgta gccaataaac
     3361 ggcagaagat tttgaccgat gatgcggtgc gtttggcttt acggcaggcg gtaatggcgg
     3421 tgcgcgaacg gtatccgttt gaaattttgg catgggtgtt gatgcccgac catctgcata
     3481 ccatatggcg gctgccggac aatgattctg cttattcgga acgctggcgg cagatcaagc
     3541 ggcacagcaa atatttaatc ggcggcaatc tcaggctttg gcaaaaacgc ttttgggaac
     3601 atactatccg cgatgaggcc gattttgcct gccattttga ttatctgcat ttcaatccgg
     3661 tcaaacatgg ctatgtagga caaatttccg attggcggtt ttctacgttt caccgttatg
     3721 tcaaacaggg tatttatccg cataattggg gtggcggcaa tgcggacttt tctattgaat
     3781 acgattaaag taatgtcgga ttcaagaatc cgacctacgg aaaactaaag attttatacg
     3841 ggctacggct tgctggctta tgcgttggct taatttgaaa aacagcacat cggttcataa
     3901 tacacaaggt attagaacaa aagaagcagt tgaacaggga ttgcgtggta ataatagacc
     3961 tgaaaaagga ataggtaata gatggaatta tgatggattt ataaaggaat tagcgaagta
     4021 acaggtattc taaatatttc tgataaatat aaagaacaag gagtaaaaaa atgaaagtaa
     4081 tcactattac attgtttact agctgcatac tatctggatg ctatgggctt gaatatggaa
     4141 tgagtggcgg cattcctcat gatgactggg caagaggtag ccatgttagt caagccaaag
     4201 cagatgctgc gtggaaagaa tgttctgaca aatatatgag aaatggtggt tcaggattag
     4261 tagaacaatg tatgaatgat aaaggattaa tacgtttatc tgaaatacgt cgtaggcaaa
     4321 agagagcaga aacacaaaga gcagtaatta agtaacaata atgaaatact actgttcccg
     4381 aacaaagcgg catttacgca ggtgatgacg gctatatttt gaaaaatgcc gtctgtaagc
     4441 aaacgtgctt cagacggcat ttttttatcc ggcggtctgt tatccgatcc gtttgaatac
     4501 caagtcccaa acgccgtgtc cgaggcgttt gccgcgcgct tcgaatttgg tttcggggcg
     4561 gtagtccggg gtgggggcgt agtctgccgc cgtattttgc aggctgtcga agctgctgag
     4621 gacttcaagc atctgctgcg catattcttc ccagtctgtc gccaagtgga tatagccgcc
     4681 ggttttgagt ttgggcagca gtttggcgat gaagggggct tgtatcagac ggcgtttgtg
     4741 gtggcgtttt ttgtgccacg ggtcggggaa gaatatgtgg atgccgtcga gcgagccgtc
     4801 ttgcagcata ttttcgacaa cttctacggc atcgtgccgc atcacgcgga tgttttccaa
     4861 atggttttct tctatgagtt tgagcagatt gcctacgccg ggaccgtgta cgtcgatggc
     4921 aagaaagtcg gtttcgggca gcctgcgggc gatttctgcg gttgccgtcc ccataccgaa
     4981 gcctatttcg aggattttgg gtcggctgct tccgaaacgg gcatcaagat cggccggtgt
     5041 tgcttggtag tcgatgccga agctgcccca taaggtatcg atggcgcgtt gctgcgcgga
     5101 ggtcatgtgg ctttggcgga ggacgaaact gcggatgctg cgggggtgtt cggcttgcca
     5161 gttgttttgt gtttggtttt cggcggtgtc tgtcataatg ctttcgtgcg cttgtgcaat
     5221 caggatgccg tctgaatgtg aaatgcctgt tcggacggcg ggaaaggaaa ggggcgatta
     5281 tacctgtttc gtttcggcag ggtaggtgta acgctgttca gacggcattt tgccactgtt
     5341 gcaacagttc ttgcagaaag cttttttgtt cgtgtccgct gtttatggct tcttgcaggt
     5401 agcggcaggc tttttcgttg tccggcccga caccgtgtcc gctgtaatgg atgcaggcga
     5461 ggttgtagaa cgccatactg tctttttttg cggcggcttt ttcaaaccat ttgcgtgctt
     5521 cattgtagtc ggcggtcata ccctgtccga agtaatacat gctgcccaga ttggtttggg
     5581 ctttgctgtg gccgagttct gcggcttctg tgtagagttt gagtgcggct gcatattcgg
     5641 gcgcgcattg taatccgtaa tgcagggctt gggctttttg ataaagccgt tctgcccttt
     5701 gccgcctgat ggcggaatct ttaatgccgc cgtattggtt aggatggttt aacgcgctgt
     5761 cggatatgag tttctgatag gcggaaaggc tgccggcttc ggctgcctgc cgataataat
     5821 gccgggcttt ttccgtatcg ggggctatgc ccaaactgta gcggcagatg tcgcccagta
     5881 tccgcaaacc ttcgggatgg cgttcggcgg cggctgcttc ggcatgatgc agggcaagtt
     5941 tgtggtttct ttccagatgc ttgccttgta ggtagatgtc ggcaagtgcg gcatgggcat
     6001 cggggtcatt ttcctgtgcc gcctgttgga accggtaaac ggcttcatcg ggagccgtag
     6061 gtaggatgag ttcggcaagc ggggtgtagg cgggaatgta tccttgttgt gcggcggcgc
     6121 gcaaatgatt gatagcttgg gtcgtgtcgg ttggtgtacc ttgtccgaaa cggtattgaa
     6181 ggccgagctg ccattgtgcc gccgccaagc cttgtgctgc agcctcgcgg tagagaaggt
     6241 gtgccgtttc aaaatccggt ggcgtaccgt actggcagta ttgggcaagc agatattgtc
     6301 cgtgggggtt gttgtttttt gcggtcgttt ttgcccattt cagggcttct tcaaaatgtc
     6361 gggacgcata cagaaggtac atcaggcgga cttgtgcttc gggcagtcct tcctctgcca
     6421 acggtctgta aagcggtatc agggtttccg ggcgcgcgcc ttccaattct tggatatatg
     6481 ccagattgta acgcgcgtag ggatgcccct gttcggcggc gatgcggagc atttccatac
     6541 cttgttcgcg gtcttggtgc gggctgtccg cgtccatcag gtgttttgct aactcgaatg
     6601 cggcggcggg atgacctgcc gaggccgctt ctgtcagcag gcggcggact tcggtaaagt
     6661 catattgttc ttgagacagg cgttctaagg cttgatgata gagtaagtcg ggattgttca
     6721 tatcagatcg ttttccgttt tgtcaaatca tcgttattcg ttatgaagtt aggtttatat
     6781 cggcacgcct gcctactgta tcatattttt tacagacgga ttttgtcttt gaaagcatgc
     6841 taatgcagat gctcgtaaat ggtttccgcc aatgccttgc tgatgccttc gacttgttcc
     6901 aaatcttctt tgctggcggc aacgacaccg cgcaggccgc cgaagcgggt gagcagggcc
     6961 tggcggcgtt tgctgcctac gccggggatg tcgcttaagg aggaggtaac gcgtgctttg
     7021 tcgcgttttt tgcggtggcc tgtaatggca aagcggtgtg attcgtcgcg cacggtttgc
     7081 aatagatgca aggccgggct gttgggcggc aggcggaaga cttcgccggt aaaaggcagt
     7141 atgagttctt ccataccggc tttgcgctca gggcctttgg cgatgccgac caagggaatg
     7201 tgcagaccga gttcttccca tacggatacg gcgacgccga tttgtccttt gccgccgtca
     7261 atcaatacga catccggcca tttgacggtc tcgccgttgg cttcggcttc ctgcattttg
     7321 ccgtaacggc gcatcaatac ttcgcgcatg gcggcgtagt cgtcgccggg cttggcggtc
     7381 gtgatgttgt agcggcggta ttgcgaaggc tggatgtttt gctcatcgta cacgacgcag
     7441 gacgcaatag tggcctcgcc ttgtgtgtgg ctgatgtcga aacattcgag gcggttgagg
     7501 ccgtctgaat ccataccgag gattttggcg agttcgtcga tacggtgttg ctggctgctt
     7561 tgttgcaggc ggcgttgggc aattgccatt tgtgcgtttt gctccgccat tttcaaccag
     7621 actttgcgtt cgcctatggt tttggtaacg aactgcattt gtttgccgtg ttcgctctct
     7681 agggcttctt tcaatgcgtc gggcatgggg aagttgctga tgatgatgtc gggtttgctt
     7741 ttgcccagat agtgttgggc gacgaaggct tcggcgtagt cttgtccgtt tggttcggga
     7801 tcgtttttgg tgtcagggaa aaaactttta tcgccgacgt gccgtccgcc gcggatgctg
     7861 acccagtgta cgcagacgat gccgtctgaa accgccagcg cgagcaggtc gatatcgttg
     7921 ggattgttcg gatttttact gtcgataaac tgattactct gcatgatgcc gagtgcttgg
     7981 atttggtcgc ggtagcgggc ggcttcttca aactgcaaat tcgcggcggc ggtctgcatt
     8041 ttgtgttgca gggtgcgggt cagttcgtcg gtcttgccgt tgagaaaagt cgaggcctcg
     8101 cgcacgctgt cgtggtagtc ttcttcgctg atatggccga cacagggtgc gctgcagcgt
     8161 ttgatttgat acagcaggca agggcggtcg cggtgttcga acacgctgtc ttcgcaggtg
     8221 cgcagcatga agactttttg caggacttgg atgctgtcgc gcacggcgtt gctgttagga
     8281 tatgggccga aatattgatt aggctttttc agcgtgccgc ggtaatacgc catttgcgga
     8341 tattgatggc cgctgagcat caaataagga tagcttttgt catcgcggaa aagaatattg
     8401 tatttgggcg acagggcttt gatgaagttg ttttcgagaa tcagggcttc ggattcggag
     8461 cgggtgatgg tggtttcgat gtggtgaacc tgtttcacca tcaatgcgat gcgcggggaa
     8521 tggtcgtttt tctggaaata gccggacacg cgccgcttga ggttgacggc tttgccgacg
     8581 tataagacgt tgccgctttc gttgaaaaaa cggtatacgc ctggcaggtt gggcaggttt
     8641 ttgaggaaaa gtgggatgtc gaagtgttcc ggaaaacggg tttctttatt catatcaatt
     8701 acttgaaaaa cggtttcaga cggcatatta ccatatcgct ggagacagcc ttatccgttc
     8761 ggacaaggct gcagggcggt tacatgctgc taatcgattt ttacccgttt caggcgcaag
     8821 gcattgctca acacggaaac cgagcttgcc gccattgccg cgccagcgat gacgggattt
     8881 aaaaagccaa gcgcggcgag aggaatgccc aagatattgt agaagaaggc gaaaaacagg
     8941 ttttgcttga tgtttttcaa agtggcttgc gacaccagca gagcatcggc gagttggttg
     9001 accgaatgct gcatcagcgt ggcggatgcg gtatgttcgg caacgtccgc tccgcctttc
     9061 atggcgaagc tgacgttagc ggcggcaagc gcgggcgcgt cgttgatgcc gtcgccgacc
     9121 atcgccacgg ttttgccggc ggctttgagt ttttgcactt cggcagcttt atcgcgcgga
     9181 ctcatgttgc cgaaggcgtg tgcgatgccc agttgtttgg cgacgtattc gaccgtgcct
     9241 tggttgtcgc cgctcataat atagacatcg atattgtgtt ttttcagacg gcctatggct
     9301 tcggcggtat cggctttcaa cgcgtcggca agtgcgaatg cgccgatggg tttgttatcg
     9361 accgagactg caacaatgct tgcaatgtcc caaacgccgt ctgaaaactc gggcaaggta
     9421 agttcggcaa attcggcttt gcctgctttc accaaaccca cgtcttccac ttcggcggca
     9481 atgcctgcgc cgacaacg
//
LOCUS       DAWHSK010000050         9299 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.050, whole genome shotgun sequence.
ACCESSION   DAWHSK010000050 DAWHSK010000000
VERSION     DAWHSK010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9299
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.050"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..214
                     /locus_tag="WIC03_000804"
     CDS             <1..214
                     /locus_tag="WIC03_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ3883334.1"
                     /translation="PLTETQTKHNRYLSKIRYVVEQSFGTLHRKFRYARAAYFGLIKV
                     SAQSHLKAMCLNLLKAANRLSAPAAA"
     gene            complement(682..1635)
                     /locus_tag="WIC03_000805"
     CDS             complement(682..1635)
                     /locus_tag="WIC03_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883335.1"
                     /translation="MHQYYDAVILSVGNGLLKRFFKQNAQLNIASRPLIITLFPGVVF
                     GDQASILSRMGADIVLYNNKHDFRIAEEYKKQYKLSCQNILYGYPIFRHASKGRHGEK
                     IYFIDQVKIPFKKEERIYTLKKLIALAEKYPEKEFTILLRVADKDITVHQDKHSYIEL
                     AKQFQLPSNLTIERKSTAQAFQEMGYCLSYSSTMLFEAECKGIPVGVVADLGFSKSYA
                     NQHFLGSGVLVYFDQIDFTSPKIADPDWLDCYATKKVITTDEFNKLLKQVVPLQHDYQ
                     EYLSAVNSIESTKTIFLRKFKKLIRDPKKFFYDSKWLRKVI"
     gene            2018..3037
                     /gene="galE"
                     /locus_tag="WIC03_000806"
     CDS             2018..3037
                     /gene="galE"
                     /locus_tag="WIC03_000806"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEZ3883336.1"
                     /translation="MKKILVTGGTGFIGSHTVVSLLKSGHQVVILDNLCNSSINILPR
                     LKTITGQEIPFYQGDIRDREILRRIFAENRIDSVIHFAGLKAVGESVAEPMKYYDNNV
                     SGSLVLAEEMARAGVFSIVFSSSATVYGDPGKVPYTEDMPLGDTTSPYGTSKSMVERI
                     LTDIQKADPRWSMILLRYFNPIGAHESGLIGEQPNGIPNNLLPYICQVAAGKLPQLAV
                     FGDDYPTPDGTGMRDYIHVMDLAEGHVAAMQAKSNVAGTHLLNLGSGRASSVLEIIRA
                     FEAASGLTIPYEVKPRRAGDLACFYADPSYTKAQIGWQTQRDLAQMMEDSWRWVSNNP
                     NGYDD"
     gene            3153..4220
                     /gene="rfbB"
                     /locus_tag="WIC03_000807"
     CDS             3153..4220
                     /gene="rfbB"
                     /locus_tag="WIC03_000807"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEZ3883337.1"
                     /translation="MRKILVTGGAGFIGSAVVRHIIRNTQDSVVNLDKLTYAGNLESL
                     TDIADNPRYAFEQVDICDRAELDRVFAQHRPDAVMHLAAESHVDRSIGSAGEFIQTNI
                     VGTFNLLEAARAYRQQMPSEQHEAFRFHHISTDEVYGDLGGTDDLFTETAPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTIVTNCSNNYGPYHFPEKLIPLMILNALDGKPLPVYG
                     DGMQIRDWLFVEDHARALYQVVTEGVVGETYNIGGHNEKANIEVVKTICALLEELAPE
                     KPAGVARYEDLITFVQDRPGHDVRYAIDTAKISRELGWKPQETFESGLHKTVQWYLDN
                     KTWWQNVLNGSYRLERLGTGK"
     gene            4283..5149
                     /gene="rfbA"
                     /locus_tag="WIC03_000808"
     CDS             4283..5149
                     /gene="rfbA"
                     /locus_tag="WIC03_000808"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEZ3883338.1"
                     /translation="MKGIILAGGSGTRLYPITRGVSKQLLPVYDKPMIYYPLSVLMLA
                     GIRDILVITAPEDNASFKRLLGDGSDFGISISYAVQPTPDGLAQAFIIGEEFIGNDNV
                     CLVLGDNIFYGQSFTQTLKQAAAQMHGATVFAYQVKNPERFGVVEFNENFRAVSIEEK
                     PQRPKSDWAVTGLYFYDNRAVEFAKQLKPSARGELEISDLNQMYLEDGSLSVQILGRG
                     FAWLDTGTHESLHEAASFVQTVQNIQNLHIACLEEIAWRNGWLSDEKLEELARPMAKN
                     QYGQYLLRLLKK"
     gene            5203..5760
                     /gene="rfbC"
                     /locus_tag="WIC03_000809"
     CDS             5203..5760
                     /gene="rfbC"
                     /locus_tag="WIC03_000809"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEZ3883339.1"
                     /translation="MNIIDTAIPDVKLLEPQVFGDARGFFMETFRDGWFKENIADCSF
                     VQENHSKSGKGVLRGLHYQTENTQGKLVRVVVGEVFDVAVDMREGSPTFGKWAGATLS
                     AQNQCQLWIPEGFAHGFYVLSDTTELTYKCTDYYKPEAEQVLIWNDPAIGIGWPLQTA
                     PLLSPKDLAGKTWAQAEKLRLTLSR"
     gene            complement(5804..7822)
                     /locus_tag="WIC03_000810"
     CDS             complement(5804..7822)
                     /locus_tag="WIC03_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HEZ3883340.1"
                     /translation="MNKSLSGSVEEYRELTLRGMILGALITVIFTASNVYLGLKVGLT
                     FASSIPAAVISMAVLKFFKGSNILENNMVQTQASAAGTLSTIIFVLPGLLMAGYWSGF
                     PFWQTTLLCIAGGILGVIFTIPLRYAMVVKSDLPYPEGVAAAEILKVGGHEEGDNRQG
                     GSGIKELAAGGALAGLMSFCAGGLRVIADSASYWFKSGTAIFQLPMGFSLALLGAGYL
                     VGLTGGIAILLGISIAWGIAVPYFSSHIPQPSDIEMAAFAMKLWKEKVRFIGAGTIGI
                     AAVWTLLMLLKPMVEGMKMSFKSFGGGAPATERAEQDLSPKAMIFWVLSMMFILGVSF
                     YHFIGDSHITGGMAWLLVVVCTLLASVIGFLVAAACGYMAGLVGSSSSPISGVGIVSI
                     VVISLVLLLVGESGGLMADEANRKFLLALTLFCGSAVICVASISNDNLQDLKTGYLLK
                     ATPWRQQVALIIGCIVGALVISPVLELLYEAYGFTGAMPREGMDAAQALAAPQATLMT
                     TIASGIFAHNLEWVYIFTGIAIGAVLIAADLVLKKSSGGKLALPVLAVGMGIYLPPSV
                     NMPIVAGAVLAAVLKHIIGKKAENREGRLKNAERIGTLFSAGLIVGESLIGVIMAFII
                     AFSVTNGGSDAPLALNLQNWDAAASWLGLAFFVTGMFFFAQRVLKAGR"
     gene            complement(8018..9139)
                     /gene="dnaJ"
                     /locus_tag="WIC03_000811"
     CDS             complement(8018..9139)
                     /gene="dnaJ"
                     /locus_tag="WIC03_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEZ3883341.1"
                     /translation="MSNQDFYATLGVARTATDEEIKKAYRKLAMKYHPDRNPDNKEAE
                     EKFKEVQKAYETLSDKEKRAMYDQYGHAAFEGGGQGGFGGFGGFGGAQGFDFGDIFSQ
                     MFGGGSGRAQPDYQGEDVQVGIEITLEEAAKGVKKRINIPTYESCDVCNGSGAKPGTS
                     PETCPTCKGSGTVHIQQAIFRMQQTCPTCHGAGKHIKEPCVKCRGAGRNKAVKAVEVN
                     IPAGIDDGQRIRLSGEGGPGMHGAPAGDLYVTVRIRAHKIFQRDGLNLHCELPISFAT
                     AALGGELEVPTLDGKVKLTVPKETQTGRRMRVKGKGVKSLRSSATGDLYCHIIVETPV
                     NLTDRQKELLEEFERISTGLENQTPRKKSFLDKLRDLFD"
BASE COUNT         2509 a         2416 c         2080 g         2294 t
ORIGIN      
        1 tccgctgacg gaaacgcaaa ccaaacataa ccgatatttg tcgaagatcc gttatgtggt
       61 cgaacaaagc ttcggtacgc tgcaccgcaa attccgctat gcgcgggcag cctatttcgg
      121 gctgattaaa gtgagtgcgc aaagccatct gaaggcgatg tgtttgaacc tgttgaaagc
      181 ggccaacagg ctaagtgcgc ccgctgccgc ctaaaaggcg gcccggatgc ctgattatcg
      241 ggtatccggg gaggattaag ggggtatttg ggtaaaatta ggaggtattt ggggcgaaaa
      301 cagctgaaaa cctgtgtttg ggtttcggct gtcgggaggg aaaggaattt tgcaaaggtc
      361 tcaggctgca tttctcaatg taaaacgcga tattttcacc cattattagg gataagtagc
      421 cagtggttta cactggctac tttcatagtt atgaagtact gggtctgagt gaattcaatt
      481 atttccgtat aaaacacagt agacatttta ctaatcggca aaattatatt agaagaattg
      541 agaactactg aagtcaggta agataccatt ttgcacaaat ttaacggcat ccctaaaaag
      601 cattttgggt ggtatttaaa ggagtacaaa atggttttaa ctacagtatg cttagaaatc
      661 tatagcctta cgcgctttat attaaatcac ctttctcaac catttagaat cataaaaaaa
      721 ctttttagga tccctaatta actttttaaa ttttcttaaa aaaatagttt tagttgattc
      781 tatactattc accgcagata aatattcttg gtaatcatgt tgcaatggca caacctgctt
      841 taatagctta ttaaactcat cagttgtaat caccttttta gtagcatagc aatcaagcca
      901 atccggatct gctatttttg gggaagtgaa atctatttga tcaaaataaa ctaaaacccc
      961 actacctaaa aaatgctgat ttgcatagga tttagaaaag cctaagtctg caacaacacc
     1021 aacagggata cccttacatt cagcttcaaa aagcatagta gatgaataag ataaacaata
     1081 ccccatttct tggaaggctt gcgcggtact ttttcgctct attgtcaaat tactcggcaa
     1141 ctgaaactgc tttgccagct ctatatacga atgtttatcc tgatgcacag taatatcttt
     1201 atctgcaacc cttagcaaaa tagtaaattc tttctcaggg tatttttcag ccaaggcaat
     1261 caattttttt aatgtataaa ttctttcttc ttttttaaat gggattttaa cttggtcaat
     1321 aaagtaaatt ttctctccat gacgaccttt tgatgcgtgg cgaaaaattg gataaccata
     1381 aagtatattt tgacaactta atttatattg tttcttatat tcctctgcaa ttctaaaatc
     1441 atgcttatta ttatataaaa caatatcagc ccccatacga gatagaatac ttgcctgatc
     1501 accgaatact acacctggaa acaaggtaat aatcaatggc cttgaagcaa tatttaattg
     1561 cgcattctgc ttaaagaacc tttttaacaa cccatttcca accgataaaa ttactgcgtc
     1621 ataatattga tgcatattct taataaagta atcatcaata tgaaaaaaaa aattgcttgg
     1681 tctttatcta tccctgattc taataactgt cgatttgaaa gaatgttttc tcgacttttg
     1741 tggatataaa tatcaatttg agcatctttt atctcttttg caacagcata gcccgagtta
     1801 aggaacgagt cataactggc aattagtaat acttttttca ctattttccc ttaattctac
     1861 accatatgaa taaaattata gtattttacc ttagatatca aatatactta accaaaaaaa
     1921 tctgaagaaa tctactgcta cttcagggat aattatttca ttaagatttt acaaccatta
     1981 ttatcaatct atcaaagtat ttttagtatt ttattctatg aaaaaaattc tcgttaccgg
     2041 cggcaccggt tttatcggct cgcataccgt tgtttctttg ctgaaaagcg gccatcaagt
     2101 cgtgattttg gataacctat gcaattccag catcaatatc ctgccacgct tgaaaacgat
     2161 taccggccaa gagattccgt tttatcaagg cgatatccgc gatcgtgaga ttttgcgccg
     2221 tatttttgcg gaaaaccgca ttgattcggt gattcatttt gccggcttga aagcggtggg
     2281 tgaaagtgtg gccgagccga tgaaatatta tgataataat gtttccggca gcttggtgtt
     2341 ggcggaagaa atggcgcgtg cgggcgtgtt tagcattgtg ttcagttctt cggcgacggt
     2401 ttatggcgat ccgggcaaag tgccttatac cgaggatatg ccactgggcg ataccaccag
     2461 cccttacggc acatcgaaat caatggttga gcgcattctc actgacattc aaaaagccga
     2521 tccgcgctgg agcatgattt tgttgcgtta tttcaatcca attggcgcgc atgaaagcgg
     2581 cttgattggc gagcagccaa acggtatccc aaataatttg ttgccttata tctgccaagt
     2641 ggcggcaggc aaactgccgc aattagcggt attcggcgat gactacccta cccctgatgg
     2701 cacgggtatg cgcgactata ttcatgtgat ggacttggct gaaggccatg tcgccgccat
     2761 gcaggcgaaa agcaatgtag caggcacgca tttgttgaac ttaggttccg gtcgcgcttc
     2821 ttcggtattg gaaatcatcc gcgcatttga agcggcttcg ggtttgacga ttccgtatga
     2881 agtcaaaccg cgccgtgccg gtgatttggc gtgcttctat gccgaccctt cctatacaaa
     2941 ggcgcaaatc ggctggcaaa cccagcgtga tttggcgcag atgatggaag actcatggcg
     3001 ttgggtcagc aacaacccaa atggctacga cgattaagtt gacctgatac aggccgtctg
     3061 aaagagatgt tttcagacgg cctctttatc tgaaaaacac acattctgtc tgctataatc
     3121 tgtttatatt ttttggctat cctctgaaat ttatgagaaa aatccttgtt accggcggcg
     3181 cgggctttat cggttctgcc gttgtccgtc atattatccg aaacacccaa gattccgtcg
     3241 tcaacctcga caaactgacc tacgcgggca atctcgaatc gctgaccgac attgccgata
     3301 atccccgcta tgctttcgag caagtggaca tttgcgaccg cgccgaactc gaccgcgtat
     3361 tcgcacaaca ccggcccgat gccgtgatgc acttggcggc ggaaagccat gtcgaccgct
     3421 ctatcggttc ggcaggcgag tttatccaaa ccaatatcgt cggcacattc aatctgcttg
     3481 aagcagcccg cgcctaccgg caacaaatgc cgtctgaaca gcacgaagcc ttccgtttcc
     3541 accatatttc caccgatgaa gtctatggcg atttaggcgg cacggacgat ttgtttaccg
     3601 aaaccgcgcc ctacgcgccg tccagcccct actctgcctc taaagcgtcc agcgaccacc
     3661 tcgtccgcgc gtggttgcgt acttacggct tgccgaccat tgtaaccaac tgctccaaca
     3721 actacggtcc ttaccatttt ccggaaaaac tcattccttt gatgattctg aacgcgcttg
     3781 acggcaaacc gctgcctgtg tacggcgacg gtatgcaaat ccgcgactgg ctgtttgtcg
     3841 aagaccacgc gcgcgcactg tatcaggttg ttaccgaagg tgttgtcggc gaaacctaca
     3901 atatcggcgg ccacaatgaa aaagccaata ttgaagtcgt caaaaccatc tgcgccctgc
     3961 tggaagaact cgctcccgaa aaaccggccg gtgtggcgcg ttatgaagat ttgattactt
     4021 tcgtacaaga ccgccccggc catgacgtac gctacgccat tgatacagcc aaaatcagta
     4081 gggaactagg ctggaaaccg caggaaacct tcgagtccgg cctccacaaa accgtgcaat
     4141 ggtatctgga caacaaaacc tggtggcaaa acgtattgaa cggcagctat cgtttggaac
     4201 gcttaggtac aggcaaatag ttttcagacg gcatcccgac gcaatgccgt ctgaaaaccc
     4261 atcgcaaagg aagaaagaaa agatgaaagg catcatactg gcaggcggca gcggcacgcg
     4321 cctctacccc atcacgcgcg gcgtatccaa acagctcctg cccgtgtacg acaaaccgat
     4381 gatttattac cccttgtcgg ttttgatgct ggcaggaatc cgcgatattt tggtgattac
     4441 cgcgcctgaa gacaacgcct ctttcaaacg cctgcttggc gacggcagcg atttcggcat
     4501 ttccatcagt tatgccgtgc aacccactcc ggacggcttg gcacaggcat ttatcatcgg
     4561 cgaagaattt atcggcaacg acaatgtttg cttggttttg ggcgacaata ttttttacgg
     4621 tcagtcgttt acgcaaacat tgaaacaggc ggcagcgcaa atgcacggcg caaccgtgtt
     4681 tgcttatcag gtcaaaaacc ccgaacgttt cggcgtggtt gaatttaacg aaaacttccg
     4741 cgccgtttcc atcgaagaaa aaccgcaacg gcccaaatcc gattgggcgg taaccggctt
     4801 gtatttctac gacaaccgcg ccgtcgagtt cgccaaacag ctcaaaccgt ccgcacgcgg
     4861 cgaattggaa atttccgacc tcaaccaaat gtatttggaa gacggttcat tgtccgtcca
     4921 aatattagga cgcggtttcg catggttgga caccggcacg cacgaaagcc tgcacgaagc
     4981 cgcttcattc gtccaaaccg tgcaaaatat ccaaaacctg cacatcgcct gcctggaaga
     5041 aatcgcttgg cgcaacggtt ggctttccga tgaaaaactg gaagaattgg cgcgcccgat
     5101 ggcgaaaaac caatacggcc aatatttgct gcgcctgttg aaaaaataat gtttgaggcc
     5161 gtctgaaact tttcagacgg cctttagatg aaagataaaa agatgaacat cattgatacc
     5221 gccattcctg acgtaaaact gcttgagccc caagtcttcg gcgacgcgcg cggctttttt
     5281 atggaaacct tccgcgacgg gtggttcaaa gaaaatattg ccgactgttc ctttgttcag
     5341 gaaaaccact ccaaatccgg caaaggcgta ttgcgcggcc tgcactacca aaccgaaaac
     5401 acacaaggca aactcgtacg cgtagttgtc ggcgaagtgt tcgacgtggc cgtcgatatg
     5461 cgcgaaggtt cgccgacttt cggcaaatgg gcgggcgcaa ccctgtccgc gcaaaaccaa
     5521 tgccagcttt ggatacccga aggcttcgcg cacgggtttt acgttttgag cgacacgacc
     5581 gaacttacct acaaatgcac ggactattac aagcctgaag ccgaacaggt tttaatatgg
     5641 aacgacccgg caatcggcat aggctggccg cttcaaaccg cgccgctgct gtcgcccaaa
     5701 gaccttgccg gcaaaacgtg ggcgcaagcc gaaaagctcc gccttacact ttcccgataa
     5761 aaaatgccgt ctgaacgttt cagacggcat tttctccgac aggctaccta cccgccttca
     5821 gtacgcgctg tgcaaagaaa aacatcccgg taacgaagaa cgccaaaccc aaccaagaag
     5881 cggcggcatc ccagttttgc agattcaacg cgagcggcgc atccgagccg ccgttggtca
     5941 cggagaaggc aataataaac gccataatca caccgatcag gctttcaccg acaatcaggc
     6001 cggcggagaa caaggttccg atgcgctcgg cgtttttcag acggccttca cggttttccg
     6061 cttttttacc gatgatgtgt ttcaacaccg ccgccaacac cgcgcctgcc acgatgggca
     6121 tattgacgga cggcggcaga taaataccca taccgaccgc aaggacgggc agggcaagtt
     6181 tgccgcctga tgattttttc aacaccaaat cggcggcaat caacaccgcg ccaatcgcga
     6241 tgccggtaaa gatatagacc cattcgaggt tgtgggcgaa aatgcccgac gcgatggtcg
     6301 tcatcaaagt cgcttgaggg gctgccaaag cctgcgccgc gtccatacct tcgcgcggca
     6361 ttgcgccggt aaagccgtag gcttcgtaaa gcagttccaa cacgggtgaa ataaccagcg
     6421 caccgacgat acagccgata atcagggcga cttgctgccg ccaaggcgtg gctttgagca
     6481 ggtagccggt tttcaggtct tgcaggttgt cattggagat cgaagccacg cagattactg
     6541 ccgagccgca aaataaagtc agtgccagca aaaatttgcg gtttgcctca tccgccatca
     6601 aaccgccgga ttcgcccacc agcagcaaaa ccagcgaaat cacgacgatg gaaacgatgc
     6661 ccacgccgga aatcgggctg gaagacgagc cgaccaaacc cgccatataa ccgcaggcgg
     6721 cggcgaccaa aaagccgatg acggaagcca aaagcgtgca aacgaccacc aaaagccaag
     6781 ccatgccgcc cgtaatgtgc gaatcgccga taaagtggta aaacgacacg cctagaataa
     6841 acatcataga caatacccag aaaatcatgg ctttaggcga caaatcctgt tcagcgcgtt
     6901 ctgtagcggg cgcaccgccg ccaaaactct tgaacgacat cttcatgcct tccaccattg
     6961 gcttgagcag catcaaaagc gtccaaaccg ccgcgatacc tatggttccc gcgccgatga
     7021 agcgtacttt ttccttccac agcttcatcg caaacgccgc catttctata tcggaaggct
     7081 gcgggatgtg cgatgaaaaa tacggcacgg caatgcccca agcaatcgaa atgcccaaca
     7141 gaatggcgat accgcccgtc agtccgacca aatagcccgc gcccaacaat gccagtgaaa
     7201 agcccatcgg cagttggaaa attgccgtac cgcttttaaa ccaataactc gcgctatcgg
     7261 caatcacacg cagacctccg gcgcaaaagc tcatcaatcc cgccaacgca ccgccggccg
     7321 ccagctcttt gatgccgctg ccgccctgac ggttatcccc ttcttcatga ccgcccactt
     7381 tcaaaatttc agcagccgcc acaccttccg gataaggcaa atcgcttttc accaccatcg
     7441 cgtaacgcag cggaatggtg aaaatcaccc ccaaaatccc gccggcaata cataaaagcg
     7501 tcgtctgcca gaacgggaaa ccgctccagt agcccgccat cagcaaaccg ggcaggacga
     7561 agatgatggt tgaaagcgta cccgcagccg aggcttgggt ctgcaccatg ttgttttcca
     7621 aaatattgct gcctttgaaa aactttaaaa ccgccatcga aatcaccgcc gccggaatcg
     7681 acgaggcaaa ggtcagcccg actttcaaac cgaggtaaac gttggacgca gtaaaaatta
     7741 cagtgatcaa tgcaccgagt atcatgcctc ggagcgtcag ttcgcggtat tcttctaccg
     7801 aaccggataa agatttattc attattcttc ctttgacaac agattcccgc atattgttgg
     7861 caatatgtca taatgtcaag ttttaaaaag aacagttaaa aacagttatc caatcctatc
     7921 tcgtactccc ttgaaaataa aaactatttt caagcaaaca aaaacagtcg tatcagggag
     7981 attccccgat acggccgcct gtttacaagc ctcaaaatca atcaaacaaa tcgcgcagct
     8041 tgtctaaaaa cgatttctta cgcggtgttt gattttccaa cccggtagaa atccgttcaa
     8101 attcttctaa aagctctttt tgacggtcgg tcaaattaac aggtgtttcg acgataatat
     8161 ggcagtacaa atcgccggtc gcgctgctgc gtaaagattt gactccctta cccttcacgc
     8221 gcatcctcct gccggtttgg gtttctttgg ggacggtgag cttgactttg ccgtccaagg
     8281 tcggcacttc caactctccg cccagagccg ccgtggcaaa actgatcggc agttcgcaat
     8341 gcaaattcaa gccgtcgcgt tggaaaatct tatgcgcccg gatgcggacg gttacataca
     8401 aatcgcctgc gggcgcgccg tgcatacccg gcccgccttc gccgctcaaa cggatacgct
     8461 gcccgtcatc gataccggcg ggaatattga cttccaccgc cttgacggcc ttattccgcc
     8521 ccgcgccacg gcatttgacg caaggttctt taatgtgttt gccggcaccg tggcaggtcg
     8581 gacaagtctg ctgcatacgg aaaatcgcct gctggatatg caccgttccc gaacctttgc
     8641 aagtcgggca ggtttccggg gatgtccccg gtttcgcacc gctgccgttg cagacatcgc
     8701 acgattcata agtcggaatg ttgatgcgtt tcttcacgcc ttttgctgct tcttcaagcg
     8761 tgatttcgat accgacttga acgtcctcgc cctgataatc aggctgggcg cgccccgaac
     8821 cgcctccaaa catttggctg aaaatatccc caaagtcaaa accttgagca ccgccgaatc
     8881 ctccaaaccc tccgaagccc ccctgtccgc cgccttcaaa cgccgcatgg ccatactggt
     8941 cgtacatagc gcgcttttct ttatcggaca aagtttcata cgccttttgt acttctttaa
     9001 acttctcttc cgcctcttta ttgtcaggat tgcggtcggg atggtatttc atcgccaatt
     9061 tacggtaggc ttttttaatt tcttcatcgg tagctgttct tgccacacct agggtggcat
     9121 aaaaatcttg attgctcatc ttttcatctg attcaaaata aaatcgtcga taaaataagg
     9181 gcaacaggac aaaacacaag gtttcccgaa caaaaaccgc ctttcagacg gcatttcttc
     9241 tgttgttcct ttaacaatgc gccgggtatc ggcggcttta cccgtccgga aattgaaaa
//
LOCUS       DAWHSK010000051         9192 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.051, whole genome shotgun sequence.
ACCESSION   DAWHSK010000051 DAWHSK010000000
VERSION     DAWHSK010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9192
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.051"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..824)
                     /locus_tag="WIC03_000812"
     CDS             complement(<1..824)
                     /locus_tag="WIC03_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEZ3883342.1"
                     /translation="MQFSYSWLKTQADTELSSDKLEHLLTMSGLEVEEAETAAPAFAG
                     VVIAEVKSVEKHPDADRLNVTRVDAGTGGLVQIVCGAPNVKAGIKVPCSLPGAVLPGN
                     FKIKPTKMRGVPSNGMLCSTNELGLPDDGVDGLHILPEDAPVGINIREYLDLDDTLFT
                     LKITPNRADCLSVKGIAREVSALTGCAFRQPEIHTAPITGSRKQPVQINAPADCGRFI
                     SRVIENVNAKAATPDWMKQRLERSGIRSISVLVDIGNYVMLEIGQPMHVFDADKLSG"
     gene            complement(849..1499)
                     /locus_tag="WIC03_000813"
     CDS             complement(849..1499)
                     /locus_tag="WIC03_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEZ3883343.1"
                     /translation="MITISNEDNMILMSRYPDKYFDLAIVDPPYGILNKTKRGGDYKF
                     NMNEYSQWDIKPDQTYFNELFRVSKNQIIWGGNYFGELWLRSEYNKGFIIWDKNQPET
                     LNNFSMAEMAWSSFDRPSKIFRFSVRKNRNKTHPTQKPVELYQWLLKMYAKQGDKILD
                     THLGSGTLAIACCIAQFDLTACEINSDYYQQSIEKIKNNLPEARISFGHPGYCIIE"
     gene            complement(1496..2965)
                     /locus_tag="WIC03_000814"
     CDS             complement(1496..2965)
                     /locus_tag="WIC03_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEZ3883344.1"
                     /translation="MTTARWVIDKHLQNFHILCKFAGILKTNSFISVEDKAKLSEKLE
                     KLDLYHRRNTGKSLDATTHKIKELSFYMFGYRDVCGQVTQKFLFSPLGNLFLKHLDNN
                     EYIQKIFLTMLWAIPFPHPYIKTDESIQLYPMRLIFKLLSDERLDCKLFSYEYIYLIS
                     FVKSADQNSYEKLVQDILVLRTCAEVKIKHQLTAENSRSHAYVNAAHEWESYFSKTLT
                     DAGVLQKTDGKIICRLKHGKTETYRKVTSSEFSIPKQLQEFVKKLQSAYSFSEMPLNL
                     NDSDRLKIDVIKEIYSFYPKELLEEIGELKDEAAYELLHLPRLIEQYADNNNGTEAYL
                     FEDVLEMGFNMFYNVEAKKIGGPGNTDLECLYITQKRKFAVEAKSTKNKLSGINSGRL
                     EDHKNKIKAIYTIVVTPRYVPAVLSDIRNCPIVIIRANTFAEFLYNCLINRSSIPEID
                     YRYFDEIIIKNLGKDISSEISNLTMQQFASNTTMEAYST"
     gene            complement(2968..4026)
                     /locus_tag="WIC03_000815"
     CDS             complement(2968..4026)
                     /locus_tag="WIC03_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEZ3883345.1"
                     /translation="MMIGASLFSSAGIAETYLHNAGIKIIAANELVPERANLYKALYP
                     ESKMIIGDILHEEVFQNLIQSVPNRLDFLIASPPCQGMSVAGKNRNIQEMANDKRNHL
                     IFRVIEFILLKRPTFVLIENVPFFLKIKLPYKGTLQTVEVILQDLFGCEYYIQTHIFD
                     SADYGVAQHRKRAIIRMNKHSTIWGMPEKVTKTISVRDAISFLPSIESGQKSNVKWHF
                     ARTHAPEHIIWLKNTPTGRSAFDNIEHYPKKKNGEKIKSYNTTYRRMEWDAPAPTITI
                     RNDAISSQLNVHPGRSMPDGTYSDARVLTPLELMLLTSLPIDWNIPDDTSELLIRQCI
                     GESIPPLLIKKIVERIGK"
     gene            complement(4209..5201)
                     /gene="pheS"
                     /locus_tag="WIC03_000816"
     CDS             complement(4209..5201)
                     /gene="pheS"
                     /locus_tag="WIC03_000816"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEZ3883346.1"
                     /translation="MENVNRIVAEGIAAVEAAQDFNALEQIKARYLGKTGELTGLLKT
                     LGQMSPEERKTIGAHINECKNRFQTAFNAKRDALNEAKLQARLAAEALDITLPGRAQE
                     GGSLHPVTLTLQRVVELFHGMGFEVADGPEIEDDFHNFQALNIPANHPARAMQDTFYV
                     ENGDVLRTHTSPIQIRYMLDKKEPPIRIIAPGRVYRVDSDATHSPMFHQAEGLWVEEG
                     VTFADLKAVFTDFIRRFFERDDLQVRFRPSFFPFTEPSAEIDIMGENGKWLEVGGCGM
                     VHPNVLKNVNIDSEKYTGFAFGIGLDRFAMLRYNVNDLRLFFDNDLNFLKQFVK"
     gene            complement(5510..5869)
                     /gene="rplT"
                     /locus_tag="WIC03_000817"
     CDS             complement(5510..5869)
                     /gene="rplT"
                     /locus_tag="WIC03_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEZ3883347.1"
                     /translation="MPRVKRGVTARARHQKIFALAKGYRGRRKNVYRVAKQAVMKAGQ
                     YAYRDRRQRKRQFRQLWIVRINAGARENGLSYSKFMNGLKRASIEIDRKVLADLAVFD
                     KAAFAQLVEKAKAALAA"
     gene            complement(5882..6079)
                     /gene="rpmI"
                     /locus_tag="WIC03_000818"
     CDS             complement(5882..6079)
                     /gene="rpmI"
                     /locus_tag="WIC03_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEZ3883348.1"
                     /translation="MPKMKTKSSAKKRFKVLGNGGVKRAHAFKRHILTKKTTKNKRQL
                     RGTSMVNDRDLASVAKMLPYA"
     gene            complement(6226..6747)
                     /gene="infC"
                     /locus_tag="WIC03_000819"
     CDS             complement(6226..6747)
                     /gene="infC"
                     /locus_tag="WIC03_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEZ3883349.1"
                     /translation="MIAQEREARINGEITAKEVRLISESGEQLGVVSVREALAMAEGQ
                     DVDLVEISPTAKPPVCKLMDYGKYKYQQAKKRDEAKKNQKQVQIKEIKFRPGTDEGDY
                     QIKMRNINRFLADGDKVKVTLRFRGREMAHQQLGAQLLERVKEDLAEVAQIESFPKME
                     GRQMVMMIAPKKK"
     gene            complement(6765..8678)
                     /gene="thrS"
                     /locus_tag="WIC03_000820"
     CDS             complement(6765..8678)
                     /gene="thrS"
                     /locus_tag="WIC03_000820"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEZ3883350.1"
                     /translation="MLNITLPDGSVRQYESPVTVAQIAASIGAGLAKAAVAGRVNGKL
                     VDACDPIVEDSAVQIITPKDQEGIEIIRHSCAHLVGHAVKQLYPNAKMVIGPVIEEGF
                     YYDIATEKPFTPEDVAAIEARMKELIAQDYDVVKIMTPRAEAIKIFQERGEEYKLRLI
                     DDMPEVEAMGMYHHQEYVDMCRGPHVPNTRFLKNFKLTKLAGAYWRGDSNNEMLQRIY
                     GTAWATKDELKAYIQRIEEAEKRDHRKLGKQLDLFHLQDEAPGMVFWHPKGWALWQVI
                     EQHMRKELNAAGYKEVKTPQIMDKTFWEKSGHWDNYKDNMFVTSSEKREYAVKPMNCP
                     GHVQIFNNGLRSYRDLPMRLAEFGSCHRNEPSGALHGLMRVRGFVQDDAHIFCTEDQI
                     VSEARAFNELLVRIYKQFGFHDVSVKLSLRPEKRAGSDDVWDKAEQGLREALTACGVE
                     WGELPGEGAFYGPKIEYHVRDALGRSWQCGTLQLDFVLPERLNAEYVTENNDRARPVM
                     LHRAILGSLERFIGILIENHAGSFPLWLAPVQMVIMNITENQADYCREVAAKLQAAGF
                     RAELDLRNEKIGYKIRDNSQYRFPYQIVVGDKEKQENKVAVRRKAEDLGSLDLDDFIA
                     QLQQEITDALVNH"
     gene            complement(8732..8808)
                     /locus_tag="WIC03_000821"
     tRNA            complement(8732..8808)
                     /locus_tag="WIC03_000821"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8772..8774),aa:Val,seq:gac)
     gene            8987..>9192
                     /locus_tag="WIC03_000822"
     CDS             8987..>9192
                     /locus_tag="WIC03_000822"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-guanine transglycosylase"
                     /protein_id="HEZ3883351.1"
                     /translation="MLKFTLHKKDGYARRGTLELNHGKIETPVFMPVGTYGSVKAMNP
                     QNLHDIKAQIILGNTYHLWLRPGL"
BASE COUNT         2566 a         2104 c         1799 g         2723 t
ORIGIN      
        1 ccggaaagtt tgtcggcatc gaaaacgtgc atcggctggc cgatttccag catcacataa
       61 ttgccgatgt ccaccagcac ggaaatactg cggatgccgc tgcgctccaa acgttgtttc
      121 atccaatcgg gagtagccgc tttcgcattg acattttcaa taacgcggct gataaaacgg
      181 ccgcaatcgg caggcgcgtt aatctgcacg ggctgttttc gactgcccgt gatcggcgcg
      241 gtatggattt cgggctgcct gaacgcgcac cccgtcaatg cggacacttc gcgcgcaatg
      301 cccttaacac tcaagcaatc agcgcggtta ggcgtaattt tcaacgtaaa cagcgtatcg
      361 tccaaatcca agtattcacg gatattgata ccgacaggcg cgtcttcagg caaaatgtgc
      421 aggccgtcta caccatcatc aggcaaacct agttcattgg tcgaacacag cataccgttt
      481 gatggcacac cgcgcatttt ggtcggcttg attttgaaat tacccggcaa aacggcaccc
      541 ggcagcgaac acggcacttt gatgcccgct ttcacattcg gcgcaccgca cacaatctgc
      601 accaacccgc ccgtacccgc atcaactcgg gtaacgttca aacggtctgc atccggatgt
      661 ttttcaacgg atttcacttc ggcaatcacc acgcccgcaa acgcaggcgc ggcagtttca
      721 gcctcttcca cttccaagcc ggacatcgtt aacagatgtt ccagcttatc ggaggaaagt
      781 tcggtatcgg cttgggtttt cagccatgag taggagaatt gcatggttaa tttctctata
      841 tttttaagtt attcaataat acaataacct ggatgcccaa aactgattct agcttcaggt
      901 aaattatttt ttattttctc aatcgattgt tggtaataat cggaattgat ttcacaagct
      961 gtcaaatcaa actgtgcaat gcagcatgca atagcaagag ttccacttcc taaatgtgta
     1021 tctaaaatct tatcaccctg ctttgcatac atttttaaca accactgata taattcgact
     1081 ggtttttgtg ttgggtgagt tttattacga tttttccgca cactaaaccg gaaaatttta
     1141 gatggcctat cgaatgacga ccaagccatt tccgccatag aaaaattatt taatgtctct
     1201 ggttgattct tatcccaaat aataaatcct ttattatatt cacttctcaa ccataactcg
     1261 ccaaaataat tcccacccca aataatttga ttttttgaca cgcgaaataa ttcattaaag
     1321 taagtttggt ctggcttaat atcccattgt gagtattcat tcatattgaa tttataatca
     1381 ccaccacgtt tagttttatt caaaatccca taaggaggat ctacaattgc caaatcaaaa
     1441 tacttgtcag gataccgaga cattaagatc atgttatctt catttgaaat agttatcatg
     1501 tactatacgc ttccattgtg gtgttacttg caaactgttg catagtcaaa ttggaaattt
     1561 ctgaactaat atcttttcca agatttttaa taataatttc atcaaaatac cgataatcaa
     1621 tctctggaat actggagcga ttaatcaaac aattatataa aaattcagca aatgtattgg
     1681 cacgaataat tacaattggg caattacgaa tatcggataa tacggcaggg acataacgtg
     1741 gtgtgacaac aattgtgtaa atggccttaa ttttattttt atgatcttcc aatcttcctg
     1801 aattaatacc tgataactta tttttagttg attttgcctc cactgcaaat tttctctttt
     1861 gcgtaatata taagcactct aaatccgtat tacctggtcc accaattttt ttagcttcta
     1921 cgttataaaa catattgaac cccatttcta gaacatcttc aaatagatat gcctctgttc
     1981 cattattatt atctgcatat tgttcaatca acctaggtaa gtgcaataat tcatatgctg
     2041 cttcatcctt aagctcacca atttcctcca ataactcttt tggatagaag ctataaattt
     2101 ccttaatgac atcaattttc aaacgatcac tatcgttcag atttaatggc atttctgaaa
     2161 acgaataagc actttgcaat tttttcacaa attcctgaag ttgcttagga atcgaaaact
     2221 cacttgatgt tactttacga tatgtttcgg tcttaccatg ctttagacgg caaataattt
     2281 taccatctgt tttttgcaaa acacctgcat cagtcaatgt ttttgaaaaa taagattccc
     2341 actcatgtgc tgcatttaca taagcatgac tacgactatt ttccgcagtt aattgatgtt
     2401 taatttttac ttcagcacat gttcgtaaca ccaaaatgtc ttgtactaat ttttcatagc
     2461 tattctgatc agcagatttc acaaatgaaa ttaaatagat atattcataa gaaaatagtt
     2521 tacaatccaa tctttcatca gataacaact taaatattag tctcatggga tataattgaa
     2581 tactttcatc agtcttaatg tacggatgag gaaatggtat cgcccacaac atagtaagaa
     2641 aaattttttg aatatattca ttattatcca agtgtttcaa aaataaatta cccaatggac
     2701 tgaacaggaa tttctgtgta acttgcccac acacatcacg ataaccaaac atatagaatg
     2761 ataattcttt tattttatga gtagtagcat ccaatgattt acctgtattt cgtctatggt
     2821 ataaatctag tttttccaat ttttcagata acttagcttt atcctctaca gatataaaag
     2881 aatttgtttt caaaatacct gcaaatttac ataaaatatg aaaattctgt aaatgtttat
     2941 ctattaccca gcgcgcagtt gtcatatcta ttttcctatt ctctctacaa tttttttaat
     3001 taacaatgga ggaatagatt caccaataca ttgccgaatt aataattctg atgtatcgtc
     3061 gggaatattc caatcaatag gtaatgatgt taaaagcatt aactctaatg gtgttaaaac
     3121 tcttgcatct gaatatgttc catcaggcat agaccgtcca ggatgaacat ttaattgtga
     3181 actgatagcg tcattacgaa tagtaatagt tggggcagga gcatcccact ccatacggcg
     3241 ataagttgta ttataacttt taattttttc accatttttt ttctttggat aatgttctat
     3301 attatcaaaa gcagatcgtc ctgttggcgt attttttagc catataatgt gctccggagc
     3361 atgtgtacgt gcaaaatgcc atttcacatt agacttttgt ccagactcaa tactaggcaa
     3421 aaaactaata gcatcacgaa cagaaatggt ttttgtaact ttttccggca ttccccaaat
     3481 agttgaatgt ttattcatac gaataatagc tcgtttacga tgttgtgcaa caccataatc
     3541 ggcagaatca aaaatatgag tttgaatata atattcgcaa ccaaataaat cttgcaaaat
     3601 tacttctact gtttgtaatg tccccttata aggtaactta atttttaaaa aaaatggaac
     3661 attttcaatc aaaacaaaag ttgggcgttt taataaaata aattcgatta ctctgaatat
     3721 taaatgatta cgtttatcat tagccatctc ttgaatgtta cgatttttcc ctgcaacact
     3781 catgccttga caaggaggag aagcaattaa aaaatctaat cgattcggca cgctctgtat
     3841 taaattttga aacacttcct catgaagtat atcacctata atcattttac tttcgggata
     3901 tagagcttta tataaattag cacgttctgg caccaattca tttgcagcta taatcttaat
     3961 acccgcatta tgcaagtagg tttctgcaat ccctgcactc gaaaacaaag aagcccctat
     4021 catcatagaa ccaaacccaa attaactata taatctctac aaagcaataa attatgcctc
     4081 tttaaacaaa attaggaatg taatttcaca acaaaatatc aaatgtttta tatcttacat
     4141 ttgaattttc ctaagcagcc tgaaaaccaa gagtaggctg cttttccata ttcaggcagc
     4201 ctacacaatc attttacaaa ctgcttcaaa aaattcaaat cgttatcaaa aaacaagcgc
     4261 aaatcgttca cgttgtaacg cagcatcgcg aaacggtcga gaccgatacc gaaagcgaaa
     4321 ccggtgtatt tttcagaatc gatattcaca tttttcaaca cgttgggatg caccatgccg
     4381 cagccgccga cttccagcca tttgccgttt tcgcccataa tgtcgatttc ggcagacggt
     4441 tcggtgaacg ggaagaaaga cgggcggaaa cgtacttgca aatcatcgcg ttcaaagaag
     4501 cgacggataa aatccgtgaa cactgctttc aaatcggcaa aagttacgcc ctcttctacc
     4561 cacaaacctt ccgcctgatg gaacataggc gagtgcgtgg catcgctgtc cacacggtaa
     4621 acgcggccgg gggcgataat gcggatgggc ggctcttttt tatcgagcat atagcggatt
     4681 tgaatcgggg aagtgtgcgt acgcaaaaca tcaccgtttt caacgtaaaa cgtatcctgc
     4741 atcgcacggg caggatggtt tgcagggatg ttcagggctt ggaaattgtg aaaatcgtct
     4801 tcgatttcag gcccgtccgc cacttcgaaa cccattccgt gaaagagttc gaccacacgt
     4861 tgcaaggtca gggttacggg atgcaggctg ccgccttcct gagcgcgtcc gggcagggta
     4921 atgtcgaggg cttcggcggc aagtcgggct tgcagcttgg cttcgttgag ggcatcgcgt
     4981 ttggcattaa aagccgtctg aaaccggttt ttgcattcat tgatatgcgc acctatggtt
     5041 ttgcgctctt caggcgacat ttgccccaaa gttttcagaa gtccggtcaa ctcgccggtt
     5101 ttgccaagat aacgggcttt gatttgttct agagcgttga agtcttgcgc agcttctact
     5161 gcggcaatgc cttctgcaac gatgcggttt acattttcca taatatcaag tctgtcaatt
     5221 aatgatatgc cacctgaaac aacgaggccg cctgtatagc ctgacatatc agactcttca
     5281 gacgacctta ttttcttcac ggtcaatcca ttggataaat tttctgtata gtggattaaa
     5341 tttaaaccag tacggcgttg cctcgccttg tcctgattta aatttaatcc actatatttt
     5401 aacgcaaatt tatttaaaaa aatagacttt aaagaaagaa aatcaatcac atagaatttc
     5461 tgcaaacaaa gaaaaaagga agccgcagct tccttttcaa ttttttggat taagcagcca
     5521 aagcagcttt ggctttttca accaattgtg caaaagcggc tttatcgaac acggccaaat
     5581 cagccaatac tttgcggtcg atttcgatag aggcgcgttt cagaccattc ataaatttgc
     5641 tgtaagacaa cccgttttca cgcgcacctg cattgatacg gacaatccac aattgacgga
     5701 attggcgttt gcgttggcgg cggtcgcggt atgcgtattg gccggctttc attaccgcct
     5761 gcttggcaac gcggtaaacg tttttacgac ggccgcgata gcctttggct aacgcgaaga
     5821 ttttttggtg acgggcacga gcggtaacac cgcgttttac gcgtggcata ttctaaactc
     5881 cttaagcgta gggtaacatt ttagcaacag aagccaaatc gcgatcattt accatagagg
     5941 taccgcgcag ttggcgtttg tttttggtgg tctttttagt caagatgtgg cgtttgaacg
     6001 catgagcgcg tttcacaccg ccgttaccca gtactttaaa gcgttttttc gcgctagact
     6061 tggttttcat tttaggcatg ggaaaactcc attcgttatc ggataaggca ttagggggtt
     6121 ttaaaacatc ggtttcaaac acttgaaccc acaatgccac ggttttttgc cgcaattgaa
     6181 aacttactcc gaagcggcaa tcggagtaag cggagaatta tagctttatt ttttcttcgg
     6241 cgcaatcatc atcaccattt ggcgaccttc cattttggga aaggactcga tttgcgccac
     6301 ttcagccaaa tcttctttta cacgttccaa aagttgcgcg ccgagttgct ggtgagccat
     6361 ttcacggccg cgaaaacgca atgtcacttt gactttatcg ccgtcggcaa ggaagcggtt
     6421 aatgttgcgc atcttgattt gataatcgcc ctcatccgta cccggacgga atttgatttc
     6481 cttgatttgc acctgctttt ggtttttctt ggcttcgtcg cgcttcttgg cttgttggta
     6541 tttgtattta ccgtaatcca tcagtttgca cacaggcggt ttagcagttg gggaaatctc
     6601 taccaaatcg acatcctgcc cttcggccat agccaaagct tcacgaactg aaacgacacc
     6661 aagctgttcg cctgactcac tgattaaacg cacttctttg gcggtaattt cgccgttgat
     6721 tcgtgcttcg cgttcttgag cgatgatgaa tactcctata aaaattaatg attgacgagg
     6781 gcatcagtga tttcttgctg caattgcgca atgaaatcat ccaaatccaa agaacccaaa
     6841 tcttctgctt tgcggcgtac cgccactttg ttttcctgct tctccttatc gccgacaacg
     6901 atttgataag ggaaacggta ttggctgttg tcgcggattt tgtaaccgat tttttcgtta
     6961 cgcaaatcca actcggcgcg gaatcctgcc gcctgcaatt tggcagccac ttcacggcag
     7021 taatccgctt gattttcggt gatattcata atcaccattt gaaccggcgc caaccataac
     7081 gggaatgagc ctgcatggtt ctcaatcaga atgccgataa accgctccaa agaacccaaa
     7141 atggcgcgat gcaacataac aggacgcgca cggtcgttgt tttcagttac atattcggca
     7201 ttcaaacgct ccggcaatac gaaatccagt tgtaatgtac cgcattgcca agaacgaccc
     7261 aaggcatctc tgacatgata ttcgatttta ggcccgtaaa acgcaccctc gcccggcaat
     7321 tcgccccatt ccacgccgca ggcagtcaat gcctcgcgca aaccctgctc tgccttatcc
     7381 cacacgtcat ctgaacctgc gcgtttttca gggcgaagag aaagcttgac ggatacatca
     7441 tggaaaccga actgtttgta gatgcgaacc aacaattcat tgaacgcacg ggcctcgctg
     7501 acgatttggt cttcggtaca gaaaatatgc gcatcatcct gcacaaatcc acggacccgc
     7561 atcagaccat gcaatgcacc gcttggttca ttgcggtggc aagaaccgaa ctcggccaaa
     7621 cgcatcggca aatcgcgata tgagcgcaaa ccgttattaa aaatttgcac atgacccgga
     7681 cagttcatcg gtttcaccgc gtattcgcgt ttttctgaac tggtcacgaa catattgtct
     7741 ttgtagttgt cccaatggcc ggatttttcc caaaaggttt tatccataat ttgaggcgtt
     7801 ttgacctctt tataaccggc ggcgttcagc tctttacgca tatgctgttc aatcacttgc
     7861 cacaaagccc agcctttagg atgccaaaac accatgcccg gcgcttcgtc ttgcagatgg
     7921 aacagatcca attgcttgcc aagtttgcgg tggtcgcgct tttcggcttc ttcgatacgt
     7981 tgaatatagg cttttaattc gtcttttgtc gcccaagccg taccgtatat gcgttgcagc
     8041 atttcattat tgctgtcgcc gcgccagtat gcgcccgcca acttggtcag tttgaagttt
     8101 ttcaggaaac gggtattcgg aacgtgcggg ccgcggcaca tatcgacata ttcctgatga
     8161 tgatacatcc ccatcgcttc cacttcgggc atatcgtcaa tcaggcgcag tttgtattct
     8221 tcgccgcgct cttgaaaaat tttaatcgcc tccgcacgcg gagtcatgat tttgaccaca
     8281 tcatagtctt gggcaatcaa ttctttcata cgcgcttcaa tggcggcaac atcttccggt
     8341 gtaaacggtt tttccgtggc aatgtcgtaa taaaagccct cttcaatgac ggggccgata
     8401 accatttttg cattaggata gagttgcttg acggcatgcc cgacaagatg ggcgcaggaa
     8461 tggcggatga tttcgatgcc ttcctgatct ttcggagtaa tgatttgaac agcagaatct
     8521 tcaacaatcg ggtcgcacgc atcgaccaat ttgccgttta ccctgcctgc caccgccgcc
     8581 ttcgccaaac cggcaccgat agacgcagca atttgagcca cggtaacggg ggattcgtat
     8641 tgtcggactg agccgtccgg caaggtaatg ttcaacatca aaaagctcca aaacattaaa
     8701 aaataacggc ataaatgccg ttatgggaat ttggtaggca cgattggatt cgaaccaacg
     8761 acccccacca tgtcaaggtg gtgctctaac caactgagct acgtgccttc aaagaatttt
     8821 tgcattttat cgggtcgttt taatttttgc aagaggtatg tgcgtttctt tatgcaacat
     8881 tgcggcaaga tttggggatg tcgtctgaaa aaccgtggga aatcatttat aatgccgtct
     8941 tttaaaacat atttcgcccc ctgtcgccca actgacattc aatcttatgc ttaaatttac
     9001 cttacacaaa aaagacggat acgcccgacg cggcacgctg gagttgaacc acggcaaaat
     9061 cgaaacgccg gtatttatgc ctgtcggtac ttacggttcg gttaaggcga tgaacccgca
     9121 aaacctgcac gacatcaagg cgcaaattat tttgggcaac acttatcatt tgtggctgcg
     9181 tccgggcttg ga
//
LOCUS       DAWHSK010000052         9096 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.052, whole genome shotgun sequence.
ACCESSION   DAWHSK010000052 DAWHSK010000000
VERSION     DAWHSK010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9096
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.052"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..1715
                     /locus_tag="WIC03_000823"
     CDS             <1..1715
                     /locus_tag="WIC03_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEZ3883352.1"
                     /translation="RLSALSGKVMALGKRRPNTKTDGVDTQNTRRMVKEAKNITAKPV
                     ALPEGSSSNRKSVAVSVAPPPKIQVSLFEDDEPRQAGEYHKPTLNLLRIPDSEPVSIN
                     PAELERTAELIESKLAEFGIGVQVVSATSGPVITRYEIEPAQGVKGSQIVALSKDLAR
                     SMSLQSVRIVETIAGKNTMGIELPNDKRQDVMLSEILSSPVFAEAKSKLTVALGKDIA
                     GTPVVGDLAKMPHLLVAGMTGSGKSVGVNGMIMSMLFKATPDEVRFIMIDPKMLELSI
                     YDGIPHLLCPVVTDMREAGQALNWCVAEMEKRYRLLSHAGVRNLEGFNQKVEAAKAAG
                     KPLLNPFSLNPDEPEPLEKLPLIVVVIDELADLMMTERKAVEQQIARLAQKARAAGIH
                     MIVATQRPSVDVVTGLIKANIPTRMAFTVQSKIDSRTILDQMGADELLKYGDSLFLQP
                     GSAEPTRLQGAFVSDDEVHQVVNYVKSQAPADYIEGLLSGEAALETANIVNPNADSDE
                     LFDQAVAYVLESKKTSISSLQRQLRIGYNRAANLMEALENAGVVSPTDLNGSRKILAH
                     KDHL"
     gene            1923..3233
                     /locus_tag="WIC03_000824"
     CDS             1923..3233
                     /locus_tag="WIC03_000824"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003742150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEZ3883353.1"
                     /translation="MSVTVETLENLERKVVLSLPWSEINAETDKKLKQTQRRAKIDGF
                     RPGKAPLKMIAQMYGASAQNDVINELVQRRFYDVAVAQELKVAGFPRFEGVEEQDDKE
                     SFKVAAIFEVFPEVVIGDLSAQEVEKVTASVGDAEVDQTVEILRKQRTRFNHVEREAQ
                     NGDRVIIDFEGKIDGEPFAGGASKNYAFVLGASQMLPEFEAGVVGMKAGESKDVTVNF
                     PEDYHGKDVAGKTAMFTITLNNVSEATLPEVDADFAKALGIADGDVAKMREEVKKNVS
                     REVERRVNEQTKESVMNALLKAVELKAPVALVNEEAARLANEMKQNFVNQGMADAANL
                     DLPLDMFKEQAERRVSLGLILAKLVDENKLEPTEEQIKAVVANFAESYEDPQEVIDWY
                     YAEPSRLQAPTSLAIESNVVDFVLGKAKVNEKALSFDEVMGAQA"
     gene            3329..3943
                     /gene="clpP"
                     /locus_tag="WIC03_000825"
     CDS             3329..3943
                     /gene="clpP"
                     /locus_tag="WIC03_000825"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEZ3883354.1"
                     /translation="MSFDNYLVPTVIEQSGRGERAFDIYSRLLKERIVFLVGPVTDES
                     ANLVVAQLLFLESENPDKDIFFYINSPGGSVTAGMSIYDTMNFIKPDVSTLCLGQAAS
                     MGAFLLSAGEKGKRFALPNSRIMIHQPLISGGLGGQASDIEIHARELLKIKEKLNRLM
                     AKHCGRDLADLERDTDRDNFMSAEEAKEYGLIDQILENRASLQF"
     gene            complement(4000..5265)
                     /gene="ispG"
                     /locus_tag="WIC03_000826"
                     /gene_synonym="gcpE"
     CDS             complement(4000..5265)
                     /gene="ispG"
                     /locus_tag="WIC03_000826"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEZ3883355.1"
                     /translation="MNTLQRRKTHQVRIDHITVGSEAPVVIQSMTNTDTADAKATALQ
                     IKELSDAGSEMVRITVNSPEAASKVAEIRRRLDDMGYATPLIGDFHFNGERLLAEFPE
                     CGKALSKYRINPGNVGKGVKGDEKFAFMIRTAAENDKAVRIGVNWGSLDQSLAKRMMD
                     ANLAFSAPKPPEEVTKEALIVSALESAEKAVLLGLPEDKIILSCKVSAVQDLIQVYRE
                     LGSRCAYPLHLGLTEAGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPGSPRTQEV
                     VVGQEILQTMGLRSFTPMVTACPGCGRTTSTVFQELAQDVQNYLRQKMSIWRTLYPGV
                     ESLNVAVMGCVVNGPGESKLADIGISLPGTGETPVAPVYVDGERKVTLKGDNIATEFL
                     AIVEEYVKTNYGENGSKRNQNKIIPIQSL"
     gene            complement(5281..6042)
                     /gene="pilW"
                     /locus_tag="WIC03_000827"
     CDS             complement(5281..6042)
                     /gene="pilW"
                     /locus_tag="WIC03_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HEZ3883356.1"
                     /translation="MPFKPSKRISLLLVLALGACSTSYRPSRAEKANQVSNIKTQLAM
                     EYMRGQDYRQATASIEDALKSDPKNELAWLVRAEIYQYLKVNDKAQESFRQALSIKPD
                     SAEINNNYGWFLCGRLNRPAESMAYFDKALADPTYPTPYIANLNKGICSAKQGQFGLA
                     EAYLKRSLAAQPQFPPAFKELARTKMLAGQLGDADYYFKKYQSRVEVLQADDLLLGWK
                     IAKALGNAQAAYEYEAQLQANFPYSEELQTVLTGQ"
     gene            complement(6045..7139)
                     /gene="rlmN"
                     /locus_tag="WIC03_000828"
     CDS             complement(6045..7139)
                     /gene="rlmN"
                     /locus_tag="WIC03_000828"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEZ3883357.1"
                     /translation="MKTNLLNYDLQGLTRHFADMGEKPFRAKQVMRWMHQSGAQNFDE
                     MTDLAKSLRHKLNEQAGIEIPKLMMSQKSSDGTRKWLLDVGTGNGVETVFIPESDRGT
                     LCISSQVGCALECTFCSTGRQGFNRNLTAAEIIGQLWWANKAMGVTPKNERVISNVVM
                     MGMGEPMANFDNVVTALSIMLDDHGYGLSRRRVTVSTSGMVPQMDRLRDVMPVALAVS
                     LHASNDEVRNQIVPLNKKYPLKELMAACQRYLVKAPRDFITFEYVMLDGINDKAQHAR
                     ELIELVTDVPCKFNLIPFNPFPNSGYERSSNENIRVFRDILQQAGFVVTVRKTRGDDI
                     DAACGQLAGQVQDKTRRQQKWQQILIGQQG"
     gene            complement(7283..7708)
                     /gene="ndk"
                     /locus_tag="WIC03_000829"
     CDS             complement(7283..7708)
                     /gene="ndk"
                     /locus_tag="WIC03_000829"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEZ3883358.1"
                     /translation="MAIERTISIIKPDAVGKNVIGKIYSRFEENGLKIVAAKMKQLTL
                     KEAQEFYAVHKDRPFYAGLVEFMTGGPVMIQVLEGENAVLKNRELMGATNPSEAAEGT
                     IRADFATSVSINAVHGSDTPENAALEIAYFFSQTEICPR"
     gene            complement(7767..8918)
                     /locus_tag="WIC03_000830"
     CDS             complement(7767..8918)
                     /locus_tag="WIC03_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AFG1 family ATPase"
                     /protein_id="HEZ3883359.1"
                     /translation="MSSRDQLFKAPPFENHSPLTWYQAASQLPNFIRDDAQTAAIEHL
                     DRLWTELMMFKRKRNRFLGRSLRSPQVPKGLYFYGGVGRGKSFLMDAFFGCLPYRRKR
                     RVHFHAFMAEIHQRLKTLKSESNPLKSVAAEIAKETRVLCFDEFHVSDIADAMILGRL
                     LENLLNEGVVLVATSNYAPSELYPQGQNRSSFLPTIALIESSLTVLNVDGGEDYRLRT
                     LRPAEIFFTPANEENEAKLAKLFKEMTGITDLNPGISTIHGREIPHKAESGRAIWFDF
                     RALCFGPRSQSDYLYLAEHYEMVFISGLEQLSPQEKAEARRLTWLIDVLYDFRVKLCA
                     TGAVDVNHIYTEGDFAEEFTRTASRMVEMQSEVYLEQPHLTLSPKASGG"
BASE COUNT         2215 a         2237 c         2392 g         2252 t
ORIGIN      
        1 accgcctgag tgccttatcc ggcaaggtca tggctttagg aaaacgccgg ccgaatacca
       61 aaacagacgg tgtcgatacc caaaatacac ggcgcatggt aaaagaagcc aagaatatta
      121 cggccaaacc cgttgccttg cccgaaggca gcagcagcaa ccgcaaatcc gtcgcggttt
      181 ccgtcgcgcc gccgcccaaa attcaggttt ctctgtttga agatgacgaa cctcggcagg
      241 cgggcgaata ccacaagcct acattgaacc tattgcggat tcccgacagc gagcccgtca
      301 gcatcaatcc cgccgaattg gagcgcactg ccgaactaat cgaatccaag ctggcagaat
      361 tcggcatcgg cgtacaagtc gtatccgcca catccggccc cgtcatcacg cgctacgaaa
      421 tcgaacccgc gcaaggtgtc aaaggcagcc aaattgttgc cttgtcgaaa gatttggcac
      481 gctctatgtc gctgcaatcc gtgcgtatcg tcgaaaccat cgccgggaaa aacacgatgg
      541 gcatcgagtt gcccaacgac aaacgccaag acgtgatgtt gagcgaaatc ttgtcctcgc
      601 ccgtgtttgc cgaagccaaa tccaagctga ccgtcgcgct gggcaaagac attgccggta
      661 cccccgttgt cggcgacttg gcgaaaatgc cgcacctttt ggtcgccggt atgactggtt
      721 cgggcaagtc cgtcggcgtg aacggcatga ttatgtctat gcttttcaaa gctacgcccg
      781 acgaagtccg cttcattatg atagacccga aaatgctcga gttgagcatt tacgacggca
      841 ttccgcacct gctctgtccc gtcgtgaccg atatgcgcga agcagggcag gcgttgaact
      901 ggtgcgtcgc cgaaatggaa aaacgctacc gcctgctttc ccacgccggt gtacgcaatc
      961 tggaaggctt caaccaaaaa gtcgaagccg caaaagcggc aggcaagcct ttgcttaatc
     1021 cgttcagcct gaaccccgac gagcccgagc cgctggaaaa actgccgttg attgtggtcg
     1081 ttatcgacga acttgccgac ctgatgatga ccgaacgcaa agccgtcgaa cagcaaatcg
     1141 cccgtctcgc ccaaaaagcg cgcgccgccg gtatccatat gattgtcgcg acccaacgtc
     1201 ccagtgtcga tgtcgttacc ggcctgatta aagccaacat cccgacgcgt atggcgttta
     1261 ccgtgcaaag caaaatcgac agccgtacca tcctcgacca aatgggcgcg gacgaactgc
     1321 tcaaatatgg cgattcgctg ttccttcagc ccggcagtgc cgaaccgacc cgcctgcaag
     1381 gcgcgtttgt ttcagacgac gaagtacatc aagtcgtcaa ctacgtcaaa tcgcaagccc
     1441 cagccgacta tattgaaggt ctgctcagcg gcgaggccgc gctggaaact gccaatatcg
     1501 ttaatccgaa tgcagacagc gacgaattgt tcgatcaggc agtcgcctat gttttggaaa
     1561 gcaaaaaaac ctccatttcg tctttgcagc ggcagttgcg tatcggctat aaccgcgcgg
     1621 caaacctgat ggaggcactg gaaaatgcgg gtgtcgtttc tcccaccgac ctcaacggca
     1681 gccgtaaaat tttggcgcac aaggaccatt tgtagcccgt attgcaaaat gccgtctgaa
     1741 cggcggaatt ggcgtttcag acggcatatt atgtttcagg cgaaacattg tgatatactt
     1801 gccagctaaa atttcccctt tgcggcaatg cggttcaaat atcgtaccgt tgcgctgttt
     1861 gcttccccat gtagggaaga aagtttatca ttttatcaac acaacaaatt taagggctta
     1921 tgatgagcgt aactgttgaa actttagaaa atctggaacg caaagtagtg ttgtccctgc
     1981 cttggtccga aatcaacgca gaaaccgata aaaaactgaa acaaactcaa cgccgtgcaa
     2041 aaatcgacgg tttccgtccg ggtaaagcac ctttaaaaat gattgcccaa atgtacggtg
     2101 cgagcgcaca aaacgacgtg atcaacgagc tggtgcaacg ccgcttctac gatgttgccg
     2161 ttgcccaaga gttgaaagtg gcaggcttcc cccgttttga aggcgttgaa gaacaagacg
     2221 ataaagagtc tttcaaagtt gccgccattt ttgaagtgtt ccccgaagtc gttatcggcg
     2281 atttgtctgc acaagaagtt gaaaaagtaa ccgcttccgt cggtgatgcc gaagtggacc
     2341 aaaccgtaga aatcctgcgc aaacaacgta cccgcttcaa ccatgtcgaa cgcgaagctc
     2401 aaaacggcga ccgcgtcatc attgactttg aaggcaaaat cgacggcgaa ccttttgcag
     2461 gcggcgcatc caaaaactac gccttcgtat tgggcgcaag tcaaatgctg cctgaatttg
     2521 aagccggcgt agtcggcatg aaggctggcg aaagtaaaga cgttaccgtc aatttccctg
     2581 aagactacca cggtaaagac gttgccggta aaactgccat gttcaccatt acgctgaaca
     2641 acgtttccga agcgactctg cctgaagtcg atgcagattt tgcaaaagcc ttgggtattg
     2701 cggatggcga cgttgccaaa atgcgcgaag aagtgaagaa aaacgtaagc cgcgaagtgg
     2761 aacgccgcgt aaacgaacaa accaaagaat ccgtaatgaa cgcgctgctc aaagccgtag
     2821 agctgaaagc acctgttgct ttggtaaatg aagaagccgc acgcttggca aacgaaatga
     2881 aacaaaattt tgttaaccaa ggtatggctg atgctgccaa cttggatctg cctttggata
     2941 tgtttaaaga acaagccgaa cgccgcgtgt ctttgggtct gattttggcc aaactggttg
     3001 atgaaaacaa actggaaccg actgaggagc aaatcaaagc cgttgtcgcc aacttcgcag
     3061 aaagctacga agatcctcaa gaagtaatcg actggtacta cgcagagcct tcccgcctgc
     3121 aagccccgac ttctttggcg atagaaagca acgtcgttga tttcgttttg ggtaaagcca
     3181 aagtaaacga aaaagctttg tcttttgacg aagtgatggg cgcgcaagcc tgattatcct
     3241 gaaaatgccg tcttagggca gtttttgaaa gcaccgaagc cgcttttaaa gcccgctttg
     3301 gtgcttttac atcatgaaag gagacgaaat gtcttttgat aactatcttg tccctaccgt
     3361 tatcgagcag agcggtcgcg gtgagcgtgc attcgatatc tattctcggc ttttgaaaga
     3421 gcgcatcgta ttcttggtcg gcccggtaac cgacgagtcc gccaatctgg tggttgccca
     3481 actgttgttt ttggaaagtg agaatccgga taaggatatt ttcttctata ttaactcgcc
     3541 aggcggttcg gtaacggccg gtatgtcgat ttacgacacc atgaatttta tcaagcccga
     3601 tgtatcgact ttgtgcttgg ggcaggcagc aagtatgggc gcgttcttat tgtcggcagg
     3661 cgaaaaaggc aaacgtttcg ccctgcccaa cagccggatt atgattcacc agcctttaat
     3721 cagcggcggc ttgggcggtc aggcatccga tattgaaatt cacgcacgcg aactgttgaa
     3781 aatcaaagaa aaactcaacc gcctgatggc gaaacattgc ggccgcgatt tggcagattt
     3841 ggagcgcgac accgaccgtg ataatttcat gtccgccgaa gaggctaaag aatatggtct
     3901 gattgatcaa atcttggaga accgtgcttc tttacagttt taataagaaa tctgagagaa
     3961 acaaatgccg tctgaaaaca gttcaggacg gcatttgtct tatagggact gtatagggat
     4021 gattttgttc tggttgcgtt tggagccgtt ttcaccatag ttggttttga catattcctc
     4081 tacaatagct aggaattcag ttgcaatatt atcacctttc agtgtgactt tgcgctcccc
     4141 gtccacataa accggcgcga cgggtgtttc ccccgtaccg ggcaggctga tgccgatgtc
     4201 ggccaatttg ctttctccgg gcccattgac aacgcagccc attacggcaa cgttcaggga
     4261 ttcaaccccg ggataaaggg tacgccatat agacattttt tggcgcaggt aattttgaac
     4321 atcttgtgcc agctcttgaa atacggtact ggtggtacgc ccgcagccgg ggcaggcggt
     4381 aaccatcggc gtaaacgaac gcaatcccat agtctgtaaa atctcttgcc cgacgacgac
     4441 ctcctgagta cgcgggctgc caggttccgg agtcagtgaa atgcggatgg tgtcgccgat
     4501 tccttcttga agcaagacgg ataatgccgc cgttgatgcg acaatgcctt tgctgcccat
     4561 accggcttcg gtcaaaccca aatgcagcgg ataggcgcaa cggctgccca gttcgcggta
     4621 aacctgaatc aaatcctgaa ccgcgctgac tttgcacgac aggatgattt tgtcttcggg
     4681 cagtcccaat agaacggctt tttcggcaga ttccaaagcg gagacaatca gtgcttcctt
     4741 cgtcacttct tcgggcggtt tcggcgcgga aaaagcgagg ttggcatcca tcatacgttt
     4801 ggcgaggctt tgatccaaag aaccccagtt tacgccgatg cggacggctt tatcgttttc
     4861 agcagcagtc cgaatcataa aggcaaattt ttcatcgcct tttacgcctt tgccgacatt
     4921 gccgggattg atgcggtatt tggacaatgc tttgccgcat tcgggaaatt ccgccaacag
     4981 gcgttcgccg ttgaagtgga aatcgccgat aagcggtgtg gcatagccca tatcgtccaa
     5041 gcggcggcgg atttcggcaa ctttggacgc ggcttcgggg ctgttgacgg taatacgcac
     5101 catttcggat ccggcatcgc tcaattcctt aatctgcaat gcggtggctt ttgcatcggc
     5161 agtgtcggtg ttggtcatag attggataac gacgggtgct tctgaaccga cggtaatatg
     5221 atcgatgcgg acttgatgcg tcttgcggcg ttggagtgtg ttcatatggt ttgaatctgt
     5281 ttattgaccg gtgaggacgg tttgcaattc ttccgagtag gggaaattcg cctgcaattg
     5341 tgcttcatat tcgtatgccg cctgtgcgtt gccgagggct ttggcaattt tccagcctag
     5401 cagcaaatca tcggcctgaa ggacttctac cctgctttgg tattttttaa agtagtaatc
     5461 ggcatcgccc aactgcccgg ccagcatttt ggtgcgcgcc agttctttaa atgcgggtgg
     5521 gaactgcggc tgggcggcga gggaacgttt caaataggct tccgccaatc cgaattgccc
     5581 ctgttttgcg ctgcatatgc ctttattcag gttggcaata taaggggtcg ggtaggtggg
     5641 gtcggccagg gctttgtcga aatatgccat agattcggca gggcggttga gcctgccgca
     5701 caggaaccag ccgtagttgt tgttgatttc ggcactgtcg ggtttgatgg agagggcttg
     5761 ccggaaactt tcctgcgcct tgtcgttaac tttcaggtat tgatagattt cggcacggac
     5821 cagccaggca agctcgtttt tagggtctga tttcaaggcg tcttcaatac ttgccgtcgc
     5881 ctgacggtag tcctgaccgc gcatatattc cattgccaac tgggttttga tattggaaac
     5941 ctgattggct ttttctgccc gcgaggggcg gtaggaagtg ctgcacgcgc ccaaggcaag
     6001 gacaagtaat aaagagattc gtttggatgg cttaaaaggc ataattaccc ctgttgtccg
     6061 attaaaatct gctgccattt ttgttggcgg cgcgttttat cctgaacctg ccccgccaac
     6121 tgtccgcagg cggcatcgat gtcgtcgccg cgcgtttttc gtacggtaac gacaaatcct
     6181 gcctgctgca aaatatcgcg gaacacacgg atgttctcat tgctggagcg ttcgtatccg
     6241 gagtttggga agggattgaa cggaatcaga ttgaacttgc agggaacatc tgtgaccagt
     6301 tcgatcagtt cgcgcgcatg ttgcgcctta tcgtttattc cgtccaacat gacgtattcg
     6361 aaagtgatga aatccctggg tgctttgacc agatagcgtt ggcatgcggc catcaattct
     6421 ttcaagggat attttttgtt caacggtacg atttggttgc ggacttcgtc attggaagcg
     6481 tggagggaaa ccgccaaagc caccggcatg acatcgcgca acctgtccat ttggggaacc
     6541 atacccgaag tggaaacggt tacgcggcgg cggctcaaac cgtagccgtg gtcgtccagc
     6601 atgatgctta aggcggtaac gacattgtcg aagttcgcca tcggctcgcc catgcccatc
     6661 atgacgacgt tggaaataac gcgctcgttt ttcggtgtaa cgcccatcgc tttgtttgcc
     6721 caccacaatt gcccgatgat ttcggcagca gtcaaattgc ggttgaagcc ctgccgaccg
     6781 gtcgaacaaa atgtacattc caaagcgcag ccgacttgtg aggaaatgca gagcgtgccg
     6841 cgatccgatt cggggatgaa gacggtttcc accccgttgc ccgtaccgac atccaaaagc
     6901 cattttcgag tgccgtctga agatttttga gacatcatca gcttgggaat ttcgatgcct
     6961 gcctgttcgt tcagtttatg gcgcaacgat tttgccaaat cggtcatttc gtcaaaattt
     7021 tgcgcgccgg attggtgcat ccaacgcata acctgtttgg cacggaaagg tttttcgccc
     7081 atatcggcaa aatgtcgggt cagcccttgg aggtcgtagt tgagcagatt ggttttcatg
     7141 tatttgtgtt tttcttaaat taaggcttca aataaaaaat tgcagggcag gcaagtttga
     7201 tttgaagcct tttgcaacag agttttcaga cggcatggat atattttatg ccgtctgaag
     7261 aggagttgtg cggtgtattg tatcaacggg ggcagatttc ggtttggctg aaaaagtagg
     7321 caatttccaa agcggcattt tccggagtgt ccgagccgtg tacggcattg atgctgaccg
     7381 aagtggcaaa gtccgcgcgt atcgtgcctt cggcggcttc ggaaggatta gttgcaccca
     7441 tcagttcgcg gtttttcaga acggcgtttt caccctctaa tacctgaatc ataaccggac
     7501 cgccggtcat aaattcaacc aatccggcgt agaaggggcg gtctttatga accgcataaa
     7561 attcttgcgc ctctttgagg gtaagctgct tcattttggc ggcaacgatt ttcagaccgt
     7621 tctcctcaaa gcggctgtat attttgccga taacgttttt gccgacggca tcgggtttga
     7681 tgatggagat ggtacgttca atcgccatgc tatatcctta tttttactta agaagaatca
     7741 aatcggtatt ctatcaaaaa aataacttat ccgcctgaag ccttgggaga tagggtcagg
     7801 tgcggctgtt ccaaataaac ttcggactgc atttcgacca tccggctggc ggtgcgggta
     7861 aattcttcgg caaaatcgcc ttctgtatag atatggttga catctaccgc gccggtggca
     7921 cacagtttga cacggaaatc gtagagtacg tcaatcagcc aagtcagccg ccgtgcctcc
     7981 gccttttctt gcggtgagag ttgttccaaa cctgaaataa aaaccatttc ataatgttcg
     8041 gccaaataca ggtagtcgga ctgtgagcgg gggccgaagc acagtgcgcg gaaatcaaac
     8101 catatggcac ggccggactc ggctttgtgg ggaatctccc gaccgtggat ggtgctgatg
     8161 ccggggttca aatcggtaat gcctgtcatt tctttgaaca gttttgccag ttttgcctca
     8221 ttttcttcat tggcaggcgt aaagaaaatc tcggcggggc ggagggtacg cagtcggtag
     8281 tcttcaccgc cgtcaacgtt taagacggtc aggctggact cgatgagcgc gattgtggga
     8341 agaaaactgc tccggttttg accttgcggg tagagttcgg aaggcgcgta gtttgaagtc
     8401 gccaccaaaa caacgccctc gttaagcagg ttttccagca gacggcctaa aatcattgca
     8461 tccgcaatat cgctgacatg aaattcgtca aaacataata cgcgggtttc tttggcaatc
     8521 tcggcggcaa cggatttcaa cgggttgctt tcgcttttca gggttttcag cctctggtgg
     8581 atttctgcca taaaggcatg aaagtggacg cggcgtttgc ggcggtaagg gaggcagccg
     8641 aaaaaagcgt ccatcagaaa gcttttgccg cgtccgaccc cgccatagaa ataaagccct
     8701 ttggggactt gcggggagcg caaactcctg cctaaaaaac ggtttctttt gcgtttgaac
     8761 atcatcaatt cggtccaaag ccgatcgagg tgttcgatgg cggctgtctg cgcgtcgtcg
     8821 cggatgaagt tgggcagttg tgaggcagcc tgataccagg tcagcgggct gtggttttca
     8881 aacggcgggg ctttaaaaag ttggtctcta ctactcatat ttaacctttt tatacttctt
     8941 gcggctaaga aagaaaatgc ttactgtcgg tattataaat gatttccttc aaacttggca
     9001 ttttccgtgg aaaagaataa tgacgcgcac aagggggtat gagcaaaggc ggcgtttcaa
     9061 tatggtgcag atatgagctc aaatgccttt tatagt
//
LOCUS       DAWHSK010000053         9059 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.053, whole genome shotgun sequence.
ACCESSION   DAWHSK010000053 DAWHSK010000000
VERSION     DAWHSK010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9059
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.053"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(175..867)
                     /locus_tag="WIC03_000831"
     CDS             complement(175..867)
                     /locus_tag="WIC03_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEZ3883360.1"
                     /translation="MKRFTYTLSDGLCIEIELKRSAKKNLILRPVNMQTVSINVPPFF
                     QDHALANWLAANETILRNTLAKTPVHPVSHPNLPEWIWYRGIKTKLDTHSQSHIRITS
                     SEILLPRKETAAQIDHLRRLLNERAREYLLPRLEKHAAETGLTPTATDLSNAKTFWGV
                     CRPHTGIRLNWRLIGTPEYVADYVCIHELCHLRHPDHSPRFWHLVNTLTPHTDNAKSW
                     LKAHGRELFVLG"
     gene            complement(975..2168)
                     /locus_tag="WIC03_000832"
     CDS             complement(975..2168)
                     /locus_tag="WIC03_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEZ3883361.1"
                     /translation="MSDQKNRRNALIGAAFLMATSAIGPGFLTQTATFTQALAASFGF
                     VILLSILLDIGAQLNIWRIVAVSEKQAQDIANQVLPGAGYFLAVLIVMGGLAFNIGNV
                     GGAGLGLNLLTGLSPETGAVISGVIAIGVFLFKEAGKVMDKFAQVMGFVMIALTVYVA
                     WQANPPLADAAVHTFMPEKLDAMAIVTLVGGTVGGYITFAGAHRLLDAGIKGKSALPE
                     VSQSSVRAILIASIMRIVLFLAVLGVVSQGVQLNPDNPASTPFEYAAGYIGLLIFGVV
                     IWAASITSVIGAAYTSVSFFSGLSPSIERNKNKWIIAFIAVSTAVFSTIGKPAQVLVF
                     VGALNGLILPISLGLILIAAYKTKIVGDYKHPLWLTVSGVIVVGLMAVLSAITISKYI
                     GGLFG"
     gene            complement(2297..3034)
                     /gene="pxpA"
                     /locus_tag="WIC03_000833"
     CDS             complement(2297..3034)
                     /gene="pxpA"
                     /locus_tag="WIC03_000833"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HEZ3883362.1"
                     /translation="MKQVDLNADLAEGCGSDEALLQLITSANIACAQHAGSIADIRAA
                     LAYAQQNGVRIGAHPGYPDRENFGRTEMNLSEADLRACLNYQLGALQALCRDQGLEMA
                     YVKPHGAMYNQAAKNRALADTVARIVADFDPKLKLMALSGSLLLEAGKAAGLGVISEV
                     FADRRYMPDGTLVPRSRPDAQVDSDEEAIAQVLQMVRDGRVKAVNGSLVAVQADSICL
                     HGDGPHAVVFAEKIRQELLAAGIKVSA"
     gene            complement(3021..3950)
                     /locus_tag="WIC03_000834"
     CDS             complement(3021..3950)
                     /locus_tag="WIC03_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HEZ3883363.1"
                     /translation="MIHVSAVQAPAHIQDTGRYGHRRYGIGHAGAMDTVALAAGNILL
                     GNDEGTAAIEIALGGIMLVFERDTPFCLTGAVYQAELDGEPVYSYWRYTARKGQTLKM
                     VRAVQGMYGYVCVAGGFDVPEVMGSRSTDLKAGFGGHQGRMLQKGDCLPIGKGAQELS
                     KVGIAPIPFTDTVHLVPSSEYAAFSEKGRLNLERETWTLQSDSNRMGYRFDGQPLTLS
                     QPLEMLSHAVQAGTVQVPPGGKPIILLADAQTTGGYPKIATVAAADLGRLAQVRFGSK
                     VKFKIIGLKEATALRRKNQVYLNQIRRITHEAG"
     gene            complement(3947..4594)
                     /gene="pxpB"
                     /locus_tag="WIC03_000835"
     CDS             complement(3947..4594)
                     /gene="pxpB"
                     /locus_tag="WIC03_000835"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HEZ3883364.1"
                     /translation="MRIEITPISESALVCRLNAPSELGKQQKLWAFAAALGQHDRIEE
                     VVVGMNNLTVFTRFDTDLATLADELQYVWEHTAVTDHQGKLVEIPVCYGGEYGPDLAE
                     VAAFHQTVISEIVRRHTAQTYTVFMMGFQPGFPYLGGLPEALHTPRRAVPRTSVPAGS
                     VGIGGSQTGVYPFASPGGWQIIGRTELPLFRADLNPPTLLAAGDQVRFVAERIEP"
     gene            4778..6985
                     /gene="uvrD"
                     /locus_tag="WIC03_000836"
     CDS             4778..6985
                     /gene="uvrD"
                     /locus_tag="WIC03_000836"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HEZ3883365.1"
                     /translation="MFPDQSAPNLLQGLNPEQLSAVTWPPQSALVLAGAGSGKTRVLT
                     TRIAWLLQTGQASVHSIMAVTFTNKAAKEMQTRLGAMIPVNVRAMWLGTFHGLCHRFL
                     RLHHRDAGLPSSFQILDSGDQLSLIKRLLKSLNIAEEIIAPRSLQGFINAQKESGLRA
                     SVLSAPDPHTSRMIECYAEYDKICQREGVVDFAELMLRSYEMLQSNEILRQHYQNRFN
                     HILVDEFQDTNKLQYAWLKLMAGGNAAVFAVGDDDQSIYRFRGANVGNMTALMEEFHI
                     DAPVKLEQNYRSVGNILAAANAVIENNDERLGKNLRTDAEAGDKIRYYSAFTDLEEAR
                     FIVDETKALEREGWDLDEIAVLYRSNAQSRVIEQSLFRSGIPYKIYGGLRFYERQEIK
                     HALAYLRLAVNPDDDNALLRVINFPPRGIGARTVENLQTASNEQGITLWQAACNAGAK
                     AAKIAAFVRLIEALRNQVGQLPLSEIIVGILKDSGLTEHYRTQKGDNQDRLDNLDELV
                     NAAIEFKPEDSNFETLPENISDDPAFPILAFLSNAALESGENQAGAGEKAVQLMTVHA
                     AKGLEFNAVFLTGMEEGRFPSEMSLAERGGLEEERRLMYVAITRARKRLYITMAQQRM
                     LHGQTQFGIVSRFVEEIPPEVLHYLSVKKPAYDSYGNTRQTATFKDKIIDDYKQPQTY
                     AGFRIGQNVRHAKFGTGVIIDAADKGESARLTINFGKQGVKELDTKFAKLEAM"
     gene            7342..7881
                     /locus_tag="WIC03_000837"
     CDS             7342..7881
                     /locus_tag="WIC03_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEZ3883366.1"
                     /translation="MSQVFKDFDLSSVWESDSWADENYKEAPFTPEILAAVESELGYK
                     LPQSFIELMAVQNGGIFVKNCFPTTQRNSWAKDHVQICEVSGIGFEKEGSLCGETGQK
                     LWMEEWEYPPIGVYFANDPSGGHAMFALDYRECGKDGEPKVVFVEQESDFEIVELAPD
                     FETFIRSLRHEDEFIDEEI"
     gene            complement(8071..8286)
                     /locus_tag="WIC03_000838"
     CDS             complement(8071..8286)
                     /locus_tag="WIC03_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883367.1"
                     /translation="MNIINYTGDDIIISLTREELQLLRSLVIEIYAGVCIDAEEFEIV
                     SGIRNSKLVQELEQQLIEAYDLMDTKG"
     gene            complement(8294..8497)
                     /locus_tag="WIC03_000839"
     CDS             complement(8294..8497)
                     /locus_tag="WIC03_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883368.1"
                     /translation="MLKRNLGFDTFKLITIFFLGIRRLQSKHIRAIWDDKSGAIVIRD
                     PNSKDGGTAFRPTLGKTYFDKQK"
     gene            complement(8410..8770)
                     /locus_tag="WIC03_000840"
                     /pseudo
     CDS             complement(8410..8770)
                     /locus_tag="WIC03_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215099.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
     gene            8823..>9059
                     /locus_tag="WIC03_000841"
     CDS             8823..>9059
                     /locus_tag="WIC03_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ3883369.1"
                     /translation="MRKSRLSRYKQNKLIELFVAGVTARTATEPDSIVYTDCYRSYDV
                     LDVSEFSHFRINHSTHFAERQNHINGIENFWNRAK"
BASE COUNT         2291 a         2484 c         2234 g         2050 t
ORIGIN      
        1 cctctttcag ttacagcagc ttgatccctt tcccttatcc aacgggggaa ggctaggata
       61 gggtggcttg caaatataca gaacaaggga caagagccac cctctctcca accctctccc
      121 tccgtacggg agggggtgga ttctcgcggg cgaagcccac gctacggtta gcctttaccc
      181 cagcacaaac aattcccgcc cgtgcgcctt cagccaactt ttagcattgt cggtatgcgg
      241 cgtcagcgtg ttcaccaaat gccaaaagcg cggactgtgg tcggggtggc ggaggtggca
      301 gagttcgtgg atgcagacat agtcggcgac gtattcgggc gtgccgatca gccgccagtt
      361 gaggcggatg ccggtgtgcg ggcggcatac gccccaaaag gttttggcgt tgctcaggtc
      421 tgtggcggtg ggcgtcagtc ctgtttcggc tgcgtgtttt tcaaggcggg gcagcaggta
      481 ttcgcgggcg cgttcgttca acaggcggcg caggtggtcg atttgtgcgg cggtttcttt
      541 tcggggaagc aggatttcag acgacgtgat acggatatgg ctttggctgt gggtatccag
      601 cttggtcttt attccccgat accaaatcca ctcgggtaag tttgggtggg aaacaggatg
      661 cacgggcgtt ttggcaagcg tgttccgcaa aatcgtttcg tttgccgcca gccagtttgc
      721 taacgcgtgg tcttgaaaaa agggtgggac gttgatgctg accgtctgca tattgacggg
      781 gcgcagaatc agatttttct tggcactgcg tttgagttcg atttcgatgc acaagccgtc
      841 ggaaagagta taggtgaagc gtttcatagt tgtgaatagg tttcagaccg gatacatcgt
      901 ctgaaacagg aattttccat atcaggcggc aaacttcgga taatatacaa aatcaaacat
      961 ctgcgctaca aggttcagcc gaacaagccg ccgatatatt tgctgatggt aatggcgctg
     1021 agtactgcca tcaaaccgac cacaatcacg ccggaaacgg tgagccacag cgggtgtttg
     1081 tagtcgccga caattttggt tttgtaggcg gcaatcagaa tcagaccgag ggaaatcggt
     1141 aaaatcaggc cgtttaatgc gcctacgaac accagcacct gcgccggttt gccgatggtg
     1201 gaaaatacgg cggtggacac ggcgataaag gcaataatcc atttgttttt attgcgttcg
     1261 atagacgggc tgagaccgga gaagaacgac accgaagtat aagccgcacc aatcaccgaa
     1321 gtaatcgaag ccgcccaaat caccacgccg aaaatcagca ggccgatgta tcccgccgca
     1381 tattcaaacg gtgtggaagc agggttgtcg ggattgagct gtacgccttg gctgaccacg
     1441 cccaaaaccg ccaaaaacaa tacaatccgc ataatcgagg caatcagaat cgcccgcacc
     1501 gagctttggc tcacttccgg caacgccgat ttgcctttga tgcctgcgtc cagcagacgg
     1561 tgcgcaccgg cgaaggtgat gtagccgccg accgtgccgc ccaccagtgt aacaatcgcc
     1621 attgcatcga gtttttccgg cataaaggta tgcacggcgg catctgccag cggcggattc
     1681 gcctgccatg ccacataaac cgtcagcgca atcattacga aacccatcac ttgggcgaat
     1741 ttgtccatca ctttgcctgc ttctttaaac agaaacacac cgatggcaat cacgccgctg
     1801 atcacggcac cggtttccgg cgacagtccg gtcagcaggt tcagacccaa gcctgcgccg
     1861 ccgacgttgc caatattgaa cgccaaaccg cccatcacaa tcagcacagc caagaaatag
     1921 cctgcgccgg gcaagacctg attggcaata tcctgcgcct gtttttcgga aacggcgaca
     1981 atccgccaaa tattgagctg cgccccgatg tcgagcagaa tcgagagcag aatcacaaag
     2041 ccgaaacttg ccgccagtgc ttgggtgaag gtggcggttt gggtcagaaa gcccgggccg
     2101 atggcggaag tcgccatcag gaatgcagcg ccgattaagg catttctgcg gtttttttga
     2161 tcagacataa tcgcttatcc tctataaaat tggttgttgc tgtgtttggg cgaaacctgc
     2221 ggttttagct acgcagaaac tcgctttgct cgttttggcg aaacctgcgg ttttcagacg
     2281 gcctatgaac tgtttttcaa gcagaaactt tgatgcctgc cgccagtagt tcctgccgga
     2341 ttttttcggc aaacaccacg gcgtgcggcc cgtctccgtg cagacagatg ctgtcggctt
     2401 gcacggcaac caggctgccg ttcactgctt tgacccgccc gtcccgcacc atctgcaata
     2461 cttgggcgat ggcttcttcg tcgctgtcca cctgcgcatc ggggcggctg cggggaacca
     2521 gcgtaccgtc gggcatatag cggcggtcgg cgaatacttc ggaaatcaca cccaagcctg
     2581 cggcttttcc ggcttccaag agcaggctgc cggaaagtgc catcaatttc aatttcgggt
     2641 cgaaatccgc cacaattcgg gcaacggtat ccgccagcgc acggtttttc gccgcttggt
     2701 tgtacattgc gccgtgcggt ttgacataag ccatttccaa accctgatca cggcacaagg
     2761 cctgcaatgc gcccaactgg taattcagac acgcccgcaa atcggcttcg gacagattca
     2821 tttcggtacg gccgaagttt tcccgatcgg gatagccggg gtgtgctccg atgcgcacgc
     2881 cgttttgttg ggcatacgcc aatgccgccc gaatatcggc aatgctgccg gcgtgttggg
     2941 cgcaggcgat gttggccgaa gtaatcagct gcaacaaggc ttcgtcgctg ccgcagcctt
     3001 cggcgagatc ggcgtttaaa tcaacctgct tcatgggtga ttctccgtat ttggttcaga
     3061 tagacttggt ttttgcgccg cagggcggtg gcttctttca agccgattat tttgaatttg
     3121 actttgctgc cgaagcgcac ctgtgccagc ctgcccaaat cggcggcggc aacggtagcg
     3181 attttcggat aaccgccggt ggtttgcgca tcggccagca ggataatcgg tttgccgccg
     3241 ggcggcacct gcacggttcc tgcctgaaca gcgtgggaca gcatttccaa aggttgcgac
     3301 agggtcagcg gctgtccgtc gaagcggtag cccatgcggt tgctatcgct ttgcagcgtc
     3361 cacgtttccc gttccagatt cagacgccct ttttcactga aagcggcata ttccgacgaa
     3421 ggaacaaggt ggacggtatc ggtaaacggt atcggggcaa tgccgacttt ggacaattcc
     3481 tgcgcacctt tgccgatggg gagacaatca cctttttgca gcattctgcc ttgatggccg
     3541 ccgaaaccgg ctttcaggtc ggtgcttctc gaacccatca cttccggcac atcaaatccg
     3601 cccgccacgc acacatagcc gtacatgccc tgcacggcac gcaccatttt caaggtctgc
     3661 cctttgcggg cggtataacg ccaatacgaa tagaccggtt cgccgtccaa ttccgcctga
     3721 tacacggcac cggtgagaca aaacggcgta tcccgttcaa acaccagcat tatcccgccc
     3781 aaagcgattt cgattgcggc cgtgccttcg tcgttgccca ataaaatatt acccgccgcc
     3841 aaagcaaccg tgtccatcgc accggcatga ccgatgccgt aacgccggtg tccgtagcgt
     3901 ccggtatcct gaatatgcgc cggtgcctgc actgccgaaa cgtgaatcat ggctcaatcc
     3961 tttctgcaac aaagcggact tggtcacccg ccgccagcag ggtcggcgga ttcaaatcgg
     4021 ctcggaacaa gggtaattcg gttctgccga taatctgcca gccgccgggc gaagcgaacg
     4081 gatacacacc ggtctgactg ccgccgatac cgaccgaacc ggcaggaacg gacgttctcg
     4141 gcacggcacg gcggggcgtg tgcaatgctt cgggcaagcc gcccagataa gggaaaccag
     4201 gctggaagcc catcataaat acggtataag tttgcgccgt atggcggcgg acgatttcgg
     4261 aaataaccgt ctgatggaaa gcagcgactt ccgccaaatc cgggccgtat tcgccgccgt
     4321 agcagacggg aatttccacc agtttgccct gatggtctgt aacggcggtg tgttcccaca
     4381 catattgcaa ttcatcggca agcgtcgcca aatcggtatc gaaacgggtg aacacggtca
     4441 gattgttcat gccgaccacc acttcctcaa tcctgtcgtg ctgcccgagc gcagcggcaa
     4501 acgcccacaa cttttgctgt ttgcccagtt cggaaggtgc attcagtcgg cagaccaaag
     4561 cggattcgct gattggtgtg atctctattc tcatttgttg ttcattttgg ttatgtttta
     4621 atgaatctat atgcaggggc ggcggtttgt caatatcttc tgtgctgcat catcaaaccg
     4681 tcgattggaa aagtgctgcc ctgccgctgc actttttcag acgaccttaa accgtttcta
     4741 ttaaaatagc gcattccact tttcagacgg catctctatg tttcccgacc aatccgcccc
     4801 caacctgctg caaggcttga accctgaaca actttccgcc gtaacctggc cgccgcaatc
     4861 cgcccttgtg ctggcaggcg cgggcagcgg caaaacgcgc gtgctgacca cgcgtatcgc
     4921 atggctgttg caaaccggtc aggcaagcgt gcatagcatt atggcggtaa cgtttaccaa
     4981 taaagccgcc aaagaaatgc agacccgttt gggcgcaatg attcccgtca acgtccgcgc
     5041 catgtggctc ggcacgtttc acggtctctg ccaccgcttt ttgcgcctgc accaccgcga
     5101 cgcaggcctg ccgtcttcct ttcaaattct cgacagcggc gaccagcttt ccctcatcaa
     5161 acgcctgctc aaaagcctca acatcgccga agaaatcatc gcaccgcgtt cgctgcaagg
     5221 ctttatcaac gcgcaaaaag aatccggttt gcgcgcttcc gtgttgagcg cgcccgaccc
     5281 gcacacaagc cgcatgatcg aatgctacgc cgaatacgac aaaatctgcc aacgcgaagg
     5341 cgtggtcgat tttgccgaac tcatgctccg cagctacgaa atgctgcaaa gcaacgaaat
     5401 cctgcgccag cattaccaaa accgcttcaa ccacattctc gtcgacgagt tccaagacac
     5461 caacaaactg caatacgcct ggctcaaact catggcgggc ggaaacgcag cggtatttgc
     5521 cgtcggcgac gacgaccaaa gcatttaccg tttccgtggc gcaaacgttg gcaatatgac
     5581 cgcgctgatg gaagaattcc acatcgacgc gcccgtcaaa ctcgaacaaa actaccgctc
     5641 cgtcggcaac atccttgccg ccgccaacgc tgttatcgaa aacaacgacg aacgcctcgg
     5701 caaaaacctg cgcaccgacg ccgaagcagg cgacaaaatt cgctactact ccgcctttac
     5761 cgacctcgaa gaagcccggt tcatcgtgga cgaaaccaaa gccctcgaac gcgaaggctg
     5821 ggatttggac gaaatcgccg tcctctaccg cagcaacgcc caatcgcgcg tcatcgaaca
     5881 aagcctgttc cgcagcggca tcccctacaa aatctacggc ggcttgcgct tttacgaacg
     5941 ccaagaaatc aaacacgcgc tcgcctacct acgcctcgcc gtcaatcccg acgacgacaa
     6001 cgccctcttg cgtgtcatca acttcccacc gcgcggcatc ggcgcacgca ccgtcgagaa
     6061 ccttcagacg gcctcaaacg aacaaggcat caccctctgg caagccgcct gcaacgccgg
     6121 cgcgaaagcc gccaaaatcg ccgccttcgt ccgcctgatt gaagcactgc gcaaccaagt
     6181 cggacaactg cccctgtccg aaatcatcgt cggcatcctc aaagacagcg gcctgaccga
     6241 acactaccga acccaaaaag gcgacaacca agaccgcctc gacaaccttg acgaactcgt
     6301 caacgccgcc atcgagttca aacccgaaga cagcaacttt gaaaccctgc ccgaaaacat
     6361 ttcagacgac cccgccttcc ccatcctcgc cttcctcagc aacgccgccc tcgaatccgg
     6421 cgaaaaccaa gcaggcgcag gtgaaaaagc cgtccaactc atgaccgtcc acgccgccaa
     6481 aggcttggaa ttcaacgccg tcttcctcac cggcatggaa gaaggccgct tccccagcga
     6541 aatgagcctt gccgaacgcg gcggcctcga agaagaacgc cgcctcatgt acgtcgccat
     6601 cacccgcgcc cgcaaacgcc tctacatcac catggcacag caacgcatgc tgcacggaca
     6661 aacccaattc ggcatcgtct cccgcttcgt cgaagaaatc ccacccgaag tattgcacta
     6721 cctgtccgtc aaaaagcctg cctacgacag ttacggcaac acgcgtcaaa ctgccacgtt
     6781 caaagacaaa atcatcgacg actacaaaca gccgcaaacc tacgcaggct tccgtatcgg
     6841 acaaaacgtc cgccacgcca aattcggcac cggcgtgatt atcgatgccg cagataaagg
     6901 cgaatccgcc cgactgacca tcaacttcgg caaacagggc gtgaaagagt tggataccaa
     6961 gtttgcgaaa ttggaagcga tgtaaatttg aaatgcaggc tggatactcg tatccgacaa
     7021 aaatatttga cgcgtctatc gtttccgaaa aaccgctgtt ggaaaatgtc ggattcgaga
     7081 atccgaccta tggcaaaaaa ctgtagcaag gacaaggcaa aaggccgtct gaatccggta
     7141 acaacaaatt ttgccgaaac ctccgccgtc attcccgcgc aggcgggaat ccagaccttt
     7201 cggcacggga acttatcgga taaaaggttt ctttagattc cacgttctag attcccgcct
     7261 gcgcgggaat gacggacggt aaaagccggg tatagatacc taacccttgt ttatagctga
     7321 aaatcaataa ggaagaacat tatgtcccaa gtttttaaag attttgattt gtcctccgta
     7381 tgggaatccg acagttgggc agatgaaaac tacaaagaag ccccgtttac ccctgaaatt
     7441 ttggctgccg tagaaagtga actgggctat aaattgccgc aaagttttat tgaattgatg
     7501 gcggtacaaa acggcggaat atttgtcaaa aactgttttc cgaccacaca gagaaattcg
     7561 tgggcgaaag atcatgtgca aatttgcgag gtatcgggca tcggctttga aaaagaaggg
     7621 agtttgtgcg gcgaaacagg gcaaaaactt tggatggaag aatgggaata tccgcccatc
     7681 ggcgtgtatt ttgccaacga cccgtcaggc ggtcatgcca tgtttgcctt ggactatcgg
     7741 gagtgtggaa aagacggcga gccgaaagtg gtgtttgtcg agcaagaatc ggattttgaa
     7801 atcgtcgaac ttgcccccga ttttgaaacc tttatccgca gcttgcggca tgaagatgag
     7861 tttattgacg aggaaatata aaacggcggt tgaaaaaccg gaatcatcaa gggaaaacgg
     7921 gcgaaataac ggataatcgc ttgaatccgt aaggaaaacg gtttggtgga acgcgccatc
     7981 caagaccttt gcaaagaaaa actgtctccg acagcattga cattattaac agaacttatc
     8041 aagtttggag ctatctcaaa tataattcgg ttatcctttt gtatccatta aatcatatgc
     8101 ttcaattaat tgttgttcta gctcttgtac caattttgaa ttgcgaattc ctgacacaat
     8161 ctcaaattct tctgcatcta tgcaaacacc tgcataaatt tcaataacaa gggaacgcaa
     8221 taattgaagc tcttctcttg ttaaagaaat aataatgtca tcacctgtgt aattgattat
     8281 attcataata attttatttt tgtttgtcaa agtaagtttt gcctaaggtt ggtctaaatg
     8341 cagttccacc atcttttgaa tttgggtctc tgattacaat tgctccagac ttatcatccc
     8401 aaattgctct tatgtgtttg gattgtaatc ttcgaattcc caagaaaaaa atcgtaataa
     8461 gtttgaaagt gtcaaatccc aagtttcttt tgagcaatat tctaatattt tatcaatttc
     8521 actttataat aatcttatga tcaaactgtt ctaatattaa tgcattagac caaaaaaaac
     8581 cttctttatt acaatgatgg gaaatccatt taggagaaca aatgcaaagt gaaaaaatag
     8641 atgagccttg ttctccttcg attccgatat ccaaatctat ccatctatgg aaattatctg
     8701 gaatttcggg ggtaaatttt tcaaaatcaa tatcatataa atttatgctt tttaaatcca
     8761 atttaatcat tagggctgtc ctagataaat agggaaattc aaattaagtt agaattatcc
     8821 ctatgagaaa aagtcgtcta agccggtata aacaaaataa actcattgag ctatttgtcg
     8881 caggtgtaac tgcaagaaca gcaacagagc ctgacagcat tgtttatacg gattgttatc
     8941 gtagctatga tgtattagat gtgagcgaat ttagccattt ccgtatcaat cacagcacac
     9001 attttgctga acgacaaaac catatcaatg gaattgagaa tttttggaac cgggcaaaa
//
LOCUS       DAWHSK010000054         8978 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.054, whole genome shotgun sequence.
ACCESSION   DAWHSK010000054 DAWHSK010000000
VERSION     DAWHSK010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8978
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.054"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..319)
                     /locus_tag="WIC03_000842"
     CDS             complement(<1..319)
                     /locus_tag="WIC03_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ3883370.1"
                     /translation="MDIYAWHGMYFFPRFPFIDIDRIRWVRVGLKAFKHYIRENGLPD
                     LIHAHRMNYAGILAQKISQKYGIPYVVTKHSSTITRGLIRPHQWQPMKNAAAHAAARL
                     AISR"
     gene            664..1368
                     /locus_tag="WIC03_000843"
     CDS             664..1368
                     /locus_tag="WIC03_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248833.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEZ3883371.1"
                     /translation="MLRSILAASLLAVSFPAAAEALNYNIVEFSESAGVEVAQDTMSA
                     RFQVAAEGRDKNAVNAEFVKKFNSFNRKLKNGSFKTELVSRSAMPRYQYTNGRRIQTG
                     WEERAEFKVEGRDFDALNRFIADVQTDASLEYTDFSVSRERRNEVIDQVSKDAILRFK
                     TRAEKLAGILGASGYKIVKLNLGHIGSHIAGGGAAQAKMLRAMPMAASVNMEGADSAA
                     QGVEEISISVNGTVQF"
     gene            complement(1433..1999)
                     /locus_tag="WIC03_000844"
     CDS             complement(1433..1999)
                     /locus_tag="WIC03_000844"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEZ3883372.1"
                     /translation="MSIKSDKWIRRMSEEFGMIDPFEPNQIKEADGKRIISYGTSSYG
                     YDIRCANEFKIFTNINSTIVDPKNFDPKNFVTVEDDCCIIPPNSFALARTVEYFRIPR
                     NVLTVCLGKSTYARCGIIVNVTPFEPEWEGYVTLEFSNTTPLPAKIYAGEGVAQVLFF
                     ESDEICETSYKDRNGKYMGQTGVTLPKA"
     gene            complement(2065..2481)
                     /locus_tag="WIC03_000845"
     CDS             complement(2065..2481)
                     /locus_tag="WIC03_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883373.1"
                     /translation="MLKHLAFLLPAMMFALPTSAAVLTSYQEPGCTYEGGVGKDGLPS
                     GKGTWRCQDGRNYTGSFKNGKFDGQGVYTVAANREIFIEPFNSDSTKFRNMVLSGTFK
                     KGLAHGRFTVSQNGETLFIMKCENGMIKEVKLPKNK"
     gene            complement(2543..3442)
                     /gene="rdgC"
                     /locus_tag="WIC03_000846"
     CDS             complement(2543..3442)
                     /gene="rdgC"
                     /locus_tag="WIC03_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HEZ3883374.1"
                     /translation="MWFKQISFYPLNKEKLPEADILADKLAEAEFTHCQGLDWFSEGF
                     TAPVSFSPELVFPADFTLRVALKKEEKVLPAGVIRDILEEKVAEIQNNEARNVGRKEK
                     QELKEQITDDLLPRAFTRSSRTEAVFNTRHGYLLVNNAASAKAENILTKLREALGGLE
                     ASLPNTKQSPSSLMTGWLLQGHCEGGFELDSDCELKGTGDIVPVVKVSKQDLTADEVV
                     QHVKNGKTVTQLGLVWREQIAFILTQDFTLKRIQYLDVLQEEAESNGDDAAGLAFASQ
                     ILMAESVSTMLEELVSYLGGWQD"
     gene            complement(3473..4930)
                     /gene="der"
                     /locus_tag="WIC03_000847"
     CDS             complement(3473..4930)
                     /gene="der"
                     /locus_tag="WIC03_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEZ3883375.1"
                     /translation="MKPTIALVGRPNVGKSTLFNRLTRTKDALVHDLPGLTRDRHYGH
                     GKVGSKPYLVIDTGGFEPVVDSGILHEMAKQTLQAVDEADAVVFLVDGRTGLTPQDKI
                     IADRLRQSPRPVYLAVNKGEGGNRAVLAAEFYELALGDPYVISGAHGDGVYYLIEDIL
                     ETFPEPEAEEADAKHPVFAVIGRPNVGKSTLVNAILGEERVIAFDMAGTTRDSIHIDF
                     EREGKPFTIIDTAGVRRRGKVDEAVEKFSVIKAMQAVEAANVAVLVLDAQQDIADQDA
                     TIAGFALEAGRALVVAVNKWDGISEERREQVKRDINRKLYFLDFAKFHFISALKERGI
                     DGLFDSIQAAYNAAMIKMPTPKITRVLQSAIERQQPPRAGLVRPKMRYAHQGGMNPPV
                     IVVHGNSLHAISDSYTRYLTQTFRKAFNLQGTPLRIQYNVSENPYENAEDKPKKKPLR
                     RVSLSNRIEKREGRKEEKNRFKKKTKVSVKKQFSK"
     gene            complement(5084..5713)
                     /locus_tag="WIC03_000848"
     CDS             complement(5084..5713)
                     /locus_tag="WIC03_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HEZ3883376.1"
                     /translation="MAAHLEEQQELDNFKYFWKTTGKWLFALLILAALGYLGYTVYQN
                     RATSQNQEAAAVLANIVEKAQNKAPQSEINAELTKLQQSYPHSISTAQATLMAAATEF
                     DAQRYDVAEGHLKWVLSNQKDSLIQALAAQRLGVVLLQQKKYDAALAALDTPVEADFA
                     PLLMETKGDVYAAQGKSQEALKNYGQALEKMPQDSVGRELVQMKLDSLK"
     gene            complement(5714..7009)
                     /gene="hisS"
                     /locus_tag="WIC03_000849"
     CDS             complement(5714..7009)
                     /gene="hisS"
                     /locus_tag="WIC03_000849"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEZ3883377.1"
                     /translation="MAQKIQSVKGMNDLLPVEQKDFKLTAAFWQAFEDTVGRWTRAYG
                     YQQIRTPIVEQTGLFVRSIGEETDVVGKEMYTFSDSNDSLSLSLRPEGTASCLRAVVE
                     HNLLYNSPQKLWYMGPMFRRERPQKGRYRQFHQVGIEALGFEGPDIDAEIIAMSADLW
                     EKLGIREYLTLEINSLGNREERAAHRAALVEYLTRYEDKLDEDSKRRLKTNPLRVLDT
                     KNPDLQEICNAAPRLVDYLGEASQNHYARFKAMLDGLGIQYIENPRLVRGLDYYNQTV
                     FEWTTDKLGAQATVCGGGRYDGLIEELGGKPAPSIGFAMGIERLLLLVSEYGSLEVNA
                     APDVYAMHQGERADLQVMKYAQALRAQGFNVMQHSGYQSLKAQMKKADNSGARFALIV
                     AQDELANGTVTLKDMNGAHGQQTVAAADLTNTLQQWKNA"
     gene            complement(7136..7678)
                     /locus_tag="WIC03_000850"
     CDS             complement(7136..7678)
                     /locus_tag="WIC03_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEZ3883378.1"
                     /translation="MQSWKERRDFNIVKQDLDFSCGAASVATLLNNFYGQTLTEEEVL
                     KKLDKEQMRASFEDMRRIMPDLGFEAKGYALSFEQLAQLKIPVIVYLKYRKDDHFSVL
                     RGIDGNTVLLADPSLGHVSMSRAQFLDAWQTREGNLAGKILAVVPKKAETISNKLFFT
                     HHPKRQTEFAVGQIRQARAE"
     gene            complement(8073..8609)
                     /locus_tag="WIC03_000851"
     CDS             complement(8073..8609)
                     /locus_tag="WIC03_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883379.1"
                     /translation="MEKIIDSKVRETNFNQAWRVIRAMQIDNISDEQVVIVRMSCYLK
                     KDEIFKNIERYGYQSSDIKKISPPFPLNILPVPKPYYFYLYDTHLNQIYDLAWSCNDF
                     LMFEIFILDRESIKYIDKDKLFKSGETLYSNFKSIKNQCKNYFFCGFDFDAPYFKSSV
                     SQVADYNFVPDAFSLFFI"
     gene            complement(8777..8977)
                     /locus_tag="WIC03_000852"
     CDS             complement(8777..8977)
                     /locus_tag="WIC03_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883380.1"
                     /translation="MQLICADWTGIGNFMKTFEKTWSAQYRDMEISVRNFWNLERTGA
                     EVYINGRRVYHNEAEMASASLR"
BASE COUNT         2123 a         2148 c         2263 g         2444 t
ORIGIN      
        1 ggcggctgat ggcgagacgt gcggcggcgt gcgccgccgc atttttcata ggctgccatt
       61 ggtggggacg gatcagaccg cgcgtaatgg tgctgctgtg tttcgtgacg acatagggga
      121 tgccgtattt ttgggaaatt ttttgggcga gtatgccggc atagttcatg cggtgggcgt
      181 gaatcaggtc gggcagcccg ttttcgcgga tgtagtgttt gaaggctttc aaacccacgc
      241 gcacccagcg gatgcggtcg atgtcgatga acggaaagcg ggggaagaaa tacatgccgt
      301 gccatgcata gatgtccaaa ccgctttgcc gatattttgc aaaaccgtaa ggtcggtcag
      361 gatgcttgct gtttctttcc gcaagtagcg gaacatcggt gcaagcacgg cggttttgat
      421 gcctttcctc tgcaatgcca gtgcctgatt ttgaaaaaaa tcccgtccac atcctgttcg
      481 gattgcggat accatgaggg gatgacgagg acgtgcaagg gtttgggcat agtgggattc
      541 cgtatcggaa aggcggttat tataagacag acgcagaccg aatatttaaa ttgttgcctt
      601 acgctaatgc aatttagcgc gccgatatgc tagattggcg gttttatgag tgaggatacg
      661 gatatgttgc gttctatttt ggcggcttcc ctattggcgg tatcttttcc ggcagcggct
      721 gaagcattga attacaatat tgtcgaattt tccgaatcgg cgggtgtcga ggtggctcag
      781 gatacaatgt ccgcacgttt ccaagtggcg gcggaaggac gggacaaaaa tgccgtcaat
      841 gccgagtttg ttaaaaaatt caacagtttt aacagaaaat tgaaaaacgg cagctttaaa
      901 accgaattgg tatcgcgcag tgcgatgccg cgctatcaat ataccaacgg cagacgcatt
      961 caaacaggct gggaggagcg tgcggaattt aaggtcgaag gtagggattt tgatgcgttg
     1021 aaccgtttta ttgctgatgt tcagacggat gcttcgcttg aatataccga tttcagcgtg
     1081 tcgcgtgaac gccgaaacga ggtcatcgat caggtcagca aggatgctat tttgcgtttt
     1141 aagacgcgtg ccgaaaaact ggcgggcatt ttgggtgcgt ccggttataa aatcgtcaaa
     1201 ttgaatttgg gacacatcgg cagccatatc gcgggtgggg gagctgctca ggcaaaaatg
     1261 cttcgtgcca tgccgatggc ggcaagcgtc aatatggagg gtgcggattc tgccgcgcag
     1321 ggtgtggagg aaatcagcat cagcgtcaat gggacggttc agttctgatt tgaggtgaac
     1381 ggtaaatgcc gtctgaaacc cgacgataag ggttcagacg gcattttgta tttcaggctt
     1441 tgggcagggt tacgccggtt tgccccatgt atttgccgtt gcggtctttg taggaagttt
     1501 cgcagatttc gtcgctctcg aaaaacagga cttgcgccac gccttcgccg gcgtagattt
     1561 tggcgggcag gggggtggtg ttggaaaact cgagggtaac gtagccttcc cattccggct
     1621 cgaacggggt aacgttgacg atgatgccgc agcgtgcgta ggtggatttg cccaagcaga
     1681 cggtcaggac gttgcgcggg atgcggaaat attcgaccgt gcgcgccagt gcgaaggaat
     1741 tgggcgggat gatgcagcag tcgtcttcaa cggtaacgaa gtttttcggg tcgaagtttt
     1801 tgggatcgac gatggtgctg ttgatgttgg tgaagatttt aaattcgttt gcgcagcgga
     1861 tgtcgtagcc gtagctggac gtaccgtagg agatgatgcg tttgccgtcg gcttctttga
     1921 tttggttcgg ctcgaaaggg tcgatcatgc cgaattcttc gctcattcgg cgtatccatt
     1981 tgtcggactt gatgctcata atgttttcct tgtttcttgc agtgttcgga caaagcattg
     2041 ggggatgccg tctgaaaacg gggcttattt gtttttgggc agcttcactt ctttaatcat
     2101 gccgttttcg catttcataa tgaagagggt ttcgccgttt tgcgagacgg taaatctgcc
     2161 gtgtgccaag ccttttttga acgtgcccga gagtaccatg ttgcggaatt tggtactgtc
     2221 ggaattgaac ggttcgataa atatttcgcg gttggcggca acggtataaa cgccttgccc
     2281 gtcgaatttg ccgtttttaa acgaaccggt atagttgcgc ccgtcttggc agcgccatgt
     2341 gcctttgcct gaaggaagcc cgtcttttcc gactccgcct tcgtaggtgc agccgggttc
     2401 ttgataggaa gtcagaacgg cggccgaagt ggggagggcg aacatcatgg cgggcagtag
     2461 gaatgcgaga tgtttaagca taagggttat tccattggat tttggttgac ggtattttgt
     2521 cgtgaaaaag ccgtctgaaa aatcaatctt gccagccgcc caaataggaa accagttctt
     2581 ccaacatggt gctgacggat tccgccatca gaatttgcga ggcgaaggca aggccggcgg
     2641 catcgtcgcc gttgctttcg gcttcttcct gcaatacgtc gaggtattgg atgcgcttga
     2701 gtgtgaagtc ttgagtgagg ataaaggcaa tttgttcgcg ccacaccaag cccagttggg
     2761 taacggtttt accgtttttg acgtgttgaa ccacttcgtc ggcggttaaa tcttgtttgg
     2821 atactttgac gacgggaaca atatcgcccg tacctttgag ttcgcaatcg ctgtctaatt
     2881 caaaaccgcc ttcgcaatgc ccttgcaaca gccagccggt catcaaggaa gagggcgatt
     2941 gcttggtatt cggcagcgag gcttccaaac cgcccaaagc ttcgcgcagc ttggtcagga
     3001 tgttttctgc tttggcggaa gccgcgttat tgacgagcag gtagccgtgg cgggtgttaa
     3061 acaccgcttc tgtacggctg ctgcgggtaa acgctcgggg cagcaggtcg tctgtaattt
     3121 gctctttaag ctcttgtttt tctttgcgac cgacattgcg ggcttcattg ttttggattt
     3181 ccgctacctt ctcttccaaa atatcgcgga tgacgccggc aggcaggact ttttcttctt
     3241 ttttcagggc gacgcgcaag gtaaagtcgg cagggaaaac gagttcaggg gagaatgaaa
     3301 ccggtgcggt aaagccttcg ctgaaccagt ctaagccttg gcaatgggta aattcagctt
     3361 cagcaagttt gtcggcaagt atgtccgcct caggcagctt ttctttgttg agcggataaa
     3421 aactaatctg cttgaaccac ataatgtttc ctattgtttg aaatgtcggg aattatttgc
     3481 tgaattgttt tttcacactg actttggttt tcttcttgaa gcggtttttc tcttctttgc
     3541 ggccttcgcg tttctcaata cggttgctca ggctgacgcg gcgcagcggt tttttcttcg
     3601 gtttgtcttc cgcattttca tacgggtttt ccgaaacatt gtattgaatc cgcaacggcg
     3661 tgccttgcag gttgaaggct ttgcggaacg tttgggtcag atagcgcgta tagctgtcgg
     3721 aaatcgcgtg cagcgaattg ccgtgtacca caattacggg agggttcatg ccgccttggt
     3781 gggcataacg cattttcgga cgcaccaagc cggcacgcgg cggttgctga cgctcgatcg
     3841 cgctttgtaa tacgcgcgtg attttcggcg tcggcatctt aatcatcgcc gcgttgtagg
     3901 cagcctgaat gctgtcaaac aaaccgtcta taccgcgctc tttcaatgcg gaaataaagt
     3961 ggaacttggc aaaatcgagg aaatacagtt tgcggttgat atcgcgtttc acttgctcgc
     4021 gacgctcttc gctgatgccg tcccatttgt tgacggcaac caccaaggcg cggcctgctt
     4081 ccaaagcaaa accggcaatc gtcgcatctt ggtcggcgat gtcctgctgc gcgtccaata
     4141 ccaaaacagc gacgtttgcc gcttcaaccg cctgcatcgc tttgataacg gagaattttt
     4201 ctactgcttc atccactttg ccgcgacggc gcacgcctgc ggtatcgatg atggtaaacg
     4261 gtttgccttc gcgttcgaaa tcgatgtgga tgctgtcgcg tgtcgtgcct gccatatcga
     4321 aagcaatcac gcgctcttcg ccgaggatgg cgttaaccag tgtggatttg ccgacgttcg
     4381 gacggccgat gacggcaaag acgggatgtt ttgcatcggc ttcttcggct tccggctctg
     4441 ggaaggtttc caaaatatct tcaatcagat aatacacgcc atcgccgtgc gcacctgaaa
     4501 taacataagg gtcgcccaaa gcaagttcgt agaactcggc ggcaagcacg gccctgttgc
     4561 cgccttcgcc tttgtttacc gccagataaa cagggcgcgg gctttggcgc aggcggtcgg
     4621 caatgatttt gtcttgcggg gtcaggccgg tacggccgtc caccaaaaac acaaccgcat
     4681 cagcttcatc gacagcctgc aaggtttgtt ttgccatttc gtgcaaaatg ccgctgtcca
     4741 caaccggctc gaaacctccg gtatcgatga ccaaataagg tttgctgccg actttgccgt
     4801 gtccgtaatg gcggtcgcgg gtcaggccgg gcaggtcgtg tacgagtgcg tctttggtgc
     4861 gcgtcaaacg gttgaataaa gtagatttgc cgacgttagg gcggccgaca agcgcgatgg
     4921 tgggtttcat tattgggtct ttctgtgtca agtgccgttc gggagaactg aacacgagca
     4981 ggtgtccgtt ggacacggta aataggttct tcacggcaag aggccataaa gagggagggt
     5041 aaaatgccgt ctgataaata atggacagca tttcagacgg catttatttc agcgaatcaa
     5101 gtttcatttg aaccaattcg cgaccgacag aatcttgagg cattttttct aaagcctgtc
     5161 cgtagttttt taaggcttcc tggctttttc cctgtgcggc atagacatcg cctttggttt
     5221 ccatcagcag gggggcgaag tccgcttcaa ccggcgtatc gagcgcggca agcgcggcat
     5281 cgtatttttt ttgttgcaac aacacaacgc ccagacgctg cgccgccaac gcttgaatca
     5341 ggctgtcttt ttggttggac aacacccatt tcaaatggcc ttcggcaaca tcgtaacgct
     5401 gtgcgtcaaa ttcggttgcc gccgccatca gcgtggcttg ggcggtggaa atggaatgcg
     5461 ggtagctttg ctggagtttg gtcaattcgg cattgatttc gctttgcggg gctttgtttt
     5521 gcgccttttc cacgatgttt gccagcaccg ccgccgcttc ctgattttgg gaagtcgcac
     5581 ggttttggta aaccgtgtat cccaagtagc cgagcgccgc caaaatcagc agggcaaaca
     5641 gccatttgcc cgtggttttc caaaaatatt taaagttgtc caactcttgt tgttcttcca
     5701 aatgggctgc catttatgcg ttcttccatt gttgtaaagt gtttgtcaaa tcagcggcgg
     5761 cgacagtttg ctgaccgtgt gcgccgttca tgtctttgag cgtaaccgta ccgttcgcca
     5821 gttcgtcttg cgccacaatc agggcaaagc gcgcgccgct gttgtcggct tttttcattt
     5881 gtgctttcag gctttgatag ccggaatgct gcattacatt gaaaccttgc gcgcgcaagg
     5941 cttgcgcgta tttcatcacc tgcaagtccg ccctttcgcc ttggtgcatt gcatagacat
     6001 caggcgcagc gttcacttcc agcgagccgt attcgctcac caaaagcagc agccgttcga
     6061 tgcccatcgc aaagccgatg gacggcgcag gtttgccgcc aagttcctca atcaaaccat
     6121 cgtaacggcc gccgccgcac acagttgcct gcgcgccgag tttgtcggtc gtccactcaa
     6181 aaaccgtctg attgtaataa tccaaaccgc gaaccaagcg cggattttca atatattgga
     6241 tacccaaacc gtccaacatc gccttgaagc gtgcatagtg gttttgcgaa gcctcgccca
     6301 aataatccac caaacgcggc gcggcgttgc agatttcctg caaatcaggg tttttcgtat
     6361 ctaaaacacg caaaggattg gttttcagac ggcgtttgct gtcttcgtcc aatttatctt
     6421 catagcgtgt caaatattca accaacgccg cacggtgtgc cgctcgctcc tcacggttgc
     6481 ccaagctgtt gatttccaaa gtcaggtatt cgcggatacc caatttttcc cataagtcgg
     6541 cagacatcgc gatgatttcc gcatcaatat ccggaccttc aaaacccaaa gcttcgatac
     6601 cgacctgatg gaactgacga taacggcctt tttgcggacg ctcgcggcgg aacatcggcc
     6661 ccatatacca cagtttttgt gggctgttgt acaaaaggtt gtgttcgacc accgcacgca
     6721 gacaagacgc cgtaccttca ggacgcaggc tcaaactcaa agaatcgttt gaatcggaga
     6781 aggtgtacat ttccttaccg accacatcgg tttcctcgcc gatggagcgg acaaacaaac
     6841 cggtttgctc gacaatcggc gtacggattt gctgataacc gtaagcgcgt gtccagcggc
     6901 cgaccgtatc ttcaaacgcc tgccaaaacg cagccgtcag tttgaaatct ttttgctcga
     6961 caggcagaag gtcgttcata cctttgacgg attggatttt ttgtgccatt tcaagtaaaa
     7021 atgcttaaat caaattgcgg gcgattatag cggattttta agggcttgtg aggatggaga
     7081 tggtttgcag acgggtaaaa atgccgtctg aaacagtttt cagacggcat ttgacttact
     7141 ctgcacgtgc ttgcctgatt tgtccgactg caaactctgt ctgccgcttg ggatgatgtg
     7201 tgaaaaacaa tttatttgaa attgtctcgg cttttttcgg cacgaccgcc aaaatcttac
     7261 ctgccaaatt tccctcacgg gtttgccaag catccaaaaa ctgcgccctg ctcattgaaa
     7321 catgccccag cgacgggtcg gcaagcaaaa ccgtattgcc gtctataccg cgcaataccg
     7381 agaaatgatc atccttgcgg tatttcagat acacgatgac ggggattttc aactgtgcaa
     7441 gctgctcgaa agacagggca tagcctttcg cctcaaaacc caaatcaggc atgatgcgcc
     7501 gcatatcctc aaacgacgcg cgcatctgct ccttatccag ctttttcaac acttcttctt
     7561 ccgtcagcgt ttgcccgtaa aaattgttca aaagcgtcgc caccgaagcc gccccgcagg
     7621 aaaaatccaa atcctgcttt acaatattga aatcgcgcct ctctttccaa ctctgcactt
     7681 tgatttttcc gtagacaaca ggattatcgt taaacatcgg cgcagcattc aaacgataag
     7741 ataaagaaac ggcaacacac gccaacagaa aaacatattt gaacttcatc atattttcca
     7801 cataaagggc agcctgaaaa tctttcaggc tgcccttgtc aaattattcc tagctttcgg
     7861 cttttttggc aaaccaaaca atccgattac ccgcataata ctttccattt attgaaatcc
     7921 aacaagccgc gcccaaaaat gccatgcact gccgatttcc gcagtaatct ttgtaccgtt
     7981 ttcttcaaat tccaaatatt cacccaataa taaacttgaa acagaacgcg tgggcagcaa
     8041 gtgcccaccc tacgcctatt caaataattt gattaaataa agaataaaga aaatgcatca
     8101 ggaacaaaat tataatctgc cacctgactc acactgcttt taaagtaagg ggcatcaaaa
     8161 tcaaaaccgc aaaaaaaata atttttgcat tgatttttaa tagatttaaa attcgaatat
     8221 agtgtttctc cagatttaaa aagtttatct ttatctatat atttgatgct ttccctatcc
     8281 aaaataaata tttcaaacat taaaaaatca ttacatgacc aagccaaatc ataaatttga
     8341 ttcaaatggg tatcataaag ataaaaataa taaggtttgg gaacaggtaa aatatttaat
     8401 ggaaaaggag gactaatttt cttaatatct gatgactgat atccatatct ttctatattc
     8461 ttaaaaattt catctttctt aagataacaa gacattctga caatcacaac ctgttcatcg
     8521 gatatattat ctatttgcat ggcgcgtata acacgccatg cctgattaaa attagtctcc
     8581 cttaccttag aatctattat cttttccaaa gatgaaaatg ccccttatct tgaactctcc
     8641 agttagaacc tggaagtttc gtacctctta aatgtgtatt aatatttctt atagtttcat
     8701 taaaatgcca cgcgctgccg atttcaacgg taattttcgt accgctttct tcaaattcca
     8761 ggtattcccc cattagctaa cgcaaagaag cagacgccat ttcggcttcg ttatgataaa
     8821 cccgccttcc gttgatatag acttccgccc ctgtccgctc caaattccaa aaattccgta
     8881 ctgaaatttc catatcccga tattgtgcag accatgtttt ttcgaaggtt ttcataaaat
     8941 ttcctatacc tgtccaatcg gcacatatca attgcatt
//
LOCUS       DAWHSK010000055         8944 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.055, whole genome shotgun sequence.
ACCESSION   DAWHSK010000055 DAWHSK010000000
VERSION     DAWHSK010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8944
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.055"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(245..2908)
                     /gene="aceE"
                     /locus_tag="WIC03_000853"
     CDS             complement(245..2908)
                     /gene="aceE"
                     /locus_tag="WIC03_000853"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEZ3883381.1"
                     /translation="MSTQLHDVDPIETQEWLDALSSVLEYEGGERAQYLLENLVKYCR
                     DKGVRMPHGTTTPYLNTVSVENEKGIPGDQNIEHRIRAFVRWNAAAIVLRAGKKDLEL
                     GGHIASFQSAATMYEVGFNHFWKAKGEGEEGDLVFFQGHVAPGIYARAFVEGRLTEDQ
                     LNNFRQEVDGHGLPSYPHPHLLPDFWQFPTVSMGLGPIMAIYQARFLKYLESRGLAKT
                     KGRKVWCFCGDGEMDEPESQGAIALAAREGLDNLIFVINCNLQRLDGPVRGNGKIIQE
                     LEGNFAGAGWNVVKVIWGRRWDRLLAKDKDGILRQRMEECLDGDYQTYKSKDGAYVRE
                     HFFNTPELKALVADMTDEQLWALNRGGHDPQKVYNAYDRAANHADGKPTVILAKTIKG
                     YGMGASGEGQNVAHQAKKMDKASLKQFRDRFDIPVTDEQIESGDLPYLTFAPDTEEYK
                     YLHARRDALGGYLPQRKPTQEVLEVPELSAFDAQLKSSGEREFSTTMAFVRILSTLLK
                     DKKIGKRVVPIVPDESRTFGMEGMFRQYGIWNPKGQQYTPQDKDQLMFYKESVDGQIL
                     QEGINEPGAMADWIAAATSYANSNFAMIPFYIYYSMFGFQRIGDLAWAAGDMHARGFL
                     LGGTAGRTTLNGEGLQHEDGHSHIQADLIPNCVSYDPTFQYEVAVIVQDGLRRMYANN
                     EDVFYYITLMNENYTHPDMPEGAEQDILKGMYLLKAGGKGDKKVQLMGSGTILQEVIA
                     GAELLKADFGVEADIWSCPSFNLLHRDAVEVERFNRLHPLEAEKVPFVTSKLQGHDGP
                     VIAATDYIRSYADRIRAYIPNDYHVLGTDGFGRSDSRANLRRFFEVDRYNVAVAALAA
                     LAEQGKVSKETVQQAIEKYGIKADSAPSWKR"
     gene            3602..5314
                     /locus_tag="WIC03_000854"
     CDS             3602..5314
                     /locus_tag="WIC03_000854"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEZ3883382.1"
                     /translation="MKASQFFISTLKEAPAEAALASHKLMIRAGLIKANASGLYTWMP
                     MGLRVLRKVENVVREEMARAGSVELLMPVVQPAELWQESGRWEFYGKELLRLKDRHDR
                     DFCMGPTCEEVIADIVRKEINSYKQLPKNFYHIQTKFRDEVRPRFGVMRAREFVMKDA
                     YSFHADYASLQTTYDAMYDAYCRIFTRLGLAFRPVAADTGSIGGTGSHEFQVLAESGE
                     DVIAYSDTSDYAANIELAPTLPLKGERTAAQAELTKVHTPNVKTIESLVEFLNIPVEQ
                     TLKSIVVEGENEGEIVLLLLRGDHEFNDIKAEKLAGVRSPLTMASPAAIVEQFGANGG
                     SLGPVSFAGKVYADFATEKGADWVIGANEDGYHYTSFNFGRDAAEPEFVDLRNVVEGD
                     ESPDGQGRLKLARGIEVGHVFQLRDKYTQAMNVSFLDNNGKSQIMEMGCYGIGITRVV
                     AAAIEQNNDEKGIIWTKAMAPFEVVIVPMNYKKSDTVREAADKIYAELLAAGADVLLD
                     DRDERAGVLLNDSELLGIPHRIVIGDRALKEGNVEYAERRNNEAQAVAIGEIVARVTA
                     LLNG"
     gene            5377..6033
                     /locus_tag="WIC03_000855"
     CDS             5377..6033
                     /locus_tag="WIC03_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEZ3883383.1"
                     /translation="MASVFLEGEAVEVGNIFCIGRNYAAHIEELKNEIPSEPVVFMKP
                     SGSILNSGGTILLPEFSRDVQFECELVLLVGKDSDGTGEGKDILGCVAGYGVGLDLTA
                     RDIQCRLKEKGLPWLKAKGFRHSACVSDFAAAGRIGNPENVLFSLKQNGVLKQRGDTG
                     LMIYPIREILHKLAADYGLGKGDLVFTGTPSGVGAIGAGDNLALELDGLVRASFTVGC
                     "
     gene            complement(6043..6498)
                     /gene="ruvX"
                     /locus_tag="WIC03_000856"
     CDS             complement(6043..6498)
                     /gene="ruvX"
                     /locus_tag="WIC03_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEZ3883384.1"
                     /translation="MHKIPKGTALAFDFGEARIGVAQGDAELGLSHPLSTVTGGSNDE
                     KFAAIAKLVQEWQPRYFVVGLPVHTDGTKHEMTHLSRKFGRRLNGRFNLPVYWVDERL
                     SSVYAESLLSEAQVFGKKRKSVLDQVAAQAILHGFFEGGPAECFNGREG"
     gene            complement(6491..7039)
                     /locus_tag="WIC03_000857"
     CDS             complement(6491..7039)
                     /locus_tag="WIC03_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEZ3883385.1"
                     /translation="MNLSNHFLVAMPDMEDAFFSQSVVYICKHDEDGALGIAINKPSP
                     ITMDMIFSATGKNIPMRMQHDSVMMGGPVQVERGYVVHTPIGNWQSSIGVSDNIALTS
                     SRDVIENISREGAVDKALISIGYSSWGKGQLERELADNAWLTVPADEHILFDIPYEHR
                     YAAAFAKLGIDPLALFSGAGHA"
     gene            complement(7041..7601)
                     /locus_tag="WIC03_000858"
     CDS             complement(7041..7601)
                     /locus_tag="WIC03_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HEZ3883386.1"
                     /translation="MNRLLLLSAAVLLTACGSGETDKIGRASTVFNILGKNDRIEVEG
                     FDDPDVQGVACYISYAKKGGLKEMVNLEEDASDASVSCVQTASSISFDETAVRKPKEV
                     FKHGASFAFKSRQIVRYYDPKRKTFAYLVYSDKIIQGSPKNSLSAVSCFGGGIPQTDG
                     VQADTSGNLLAGACMISNPIENPDKR"
     gene            7929..>8944
                     /locus_tag="WIC03_000859"
     CDS             7929..>8944
                     /locus_tag="WIC03_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883387.1"
                     /translation="MRYKPLLLALMLVFSTPAVAAHDAAHNRSAEVKKQTKNKKEQPE
                     AAEGKKEKGKNGAVKDKKTGGKEAAKEGKESKKTAKNRKEAEKEATSRQSVRKGHEGD
                     KKSKAEHKKAHGKPVSGSKEKNAKTQPENKQGKKEAKGQGNPRKGGKAEKDTVSANKK
                     VRSDKNGKAVKQDKKYREEKNAKTDSDELKAAVAAATNDVENKKALLKQSEGMLLHVS
                     NSLKQLQEERIRQERIRQERIRQARGNLASVNRKQREAWDKFQKLNTELNRLKTEVAA
                     TKAQISRFVSGNYKNSQPNAVALFLKNAEPGQKNRFLRYTRYVNASNREVVKDLEKQQ
                     KALAV"
BASE COUNT         2162 a         2201 c         2455 g         2126 t
ORIGIN      
        1 acggcgatgg tgtcgcccgc tttaacttct acggcgatga tgtcgacgtt ttcgtgaccg
       61 ccgatatcgg ggactttgat ttctacgata ctcatttgag ttcctttaat cttgttcagt
      121 tggatgccgt ctgaaagctg cttcaggcgg catcggtacg gtttgtctgc tcaaaccatt
      181 cgggcattcg gttttcagac ggcctgatgt cggaatgggg caaacaggcc gtctgaaaca
      241 tcaatcagcg tttccagcta ggagctgaat cggctttgat gccgtatttc tcaatggctt
      301 gttgaacggt ttctttgctg actttgcctt gttccgccaa tgcggccaat gcggccacgg
      361 caacgttgta gcgatccact tcaaagaagc ggcgcaggtt ggcgcggctg tcggagcggc
      421 cgaagccgtc agtacccaag acgtggtagt cgttcgggat gtaggcgcgg atacggtcag
      481 catagctgcg gatatagtcg gtagcggcaa taaccggacc gtcatgacct tgcaatttgg
      541 aagtaacgaa aggtactttt tcggcttcca gcggatgcag gcggttgaag cgttctacct
      601 cgacagcgtc gcggtgcagc aggttgaagg atgggcaaga ccaaatgtct gcttccacgc
      661 cgaagtcggc tttcagcagt tcggcaccgg caatcacttc ttgcaaaatc gtaccggaac
      721 ccatcagttg gactttcttg tcgcctttgc cgccggcttt cagcaggtac atacctttca
      781 agatgtcttg ttccgcacct tcgggcatat ccggatgggt gtagttctcg ttcatcaggg
      841 tgatgtagta gaacacgtct tcattattgg catacatacg gcgcagaccg tcttgtacga
      901 tgacggcgac ttcgtattgg aaagtcgggt cataagatac gcagttcgga atcaggtcgg
      961 cctggatgtg gctgtggccg tcttcgtgtt gcaggccttc gccgttcagc gtcgtacggc
     1021 cggcagtacc gcccagcagg aagccgcgcg cgtgcatatc gcccgccgcc caagccaagt
     1081 cgccgatacg ttggaaaccg aacatagaat agtaaatgta gaacggaatc atggcgaagt
     1141 tgctgttggc gtagctggtt gcagccgcaa tccagtcggc catcgcgccc ggttcgttaa
     1201 taccttcttg caagatttga ccgtcaacgg attctttata gaacatcagt tggtctttgt
     1261 cttgaggggt atattgctga cctttcggat tccaaatacc gtattggcgg aacatacctt
     1321 ccatgccgaa agtacggctt tcgtcgggaa cgataggtac gacgcgtttg ccgatttttt
     1381 tgtctttcag caaagtggac aggatgcgga cgaatgccat cgtggtcgag aactcgcgtt
     1441 caccgctgga tttgagttgt gcgtcgaatg ctgacagctc gggcacttcc aatacttcct
     1501 gcgtcggttt gcgttgcggc aggtagccgc ccaaagcatc gcggcgtgcg tgcaggtatt
     1561 tgtattcttc cgtatcgggg gcaaaagtca ggtaaggcag atcgccgctt tcgatttgtt
     1621 cgtcggtaac cggaatgtca aagcggtcgc ggaattgttt cagggacgct ttgtccattt
     1681 ttttggcttg gtgggcaacg ttctgacctt cgccggatgc gcccataccg taacctttaa
     1741 tggttttcgc caagatgacg gtaggtttgc cgtcggcatg gttcgctgcg cggtcgtagg
     1801 cgttgtacac tttttgcggg tcgtggccgc cgcggttcaa tgcccagagt tgctcatcgg
     1861 tcatatcggc aaccaatgct ttcagttcgg gcgtattgaa gaagtgttcg cgcacatacg
     1921 cgccgtcttt ggatttgtaa gtttggtagt cgccgtccaa acattcttcc atacgttggc
     1981 gcaggatacc gtctttgtct ttcgccaaga ggcggtccca gcggcggccc caaatgactt
     2041 tgacgacatt ccagccggcg ccggcaaagt tgccttccaa ttcttggatg attttgccgt
     2101 tgccgcgtac cggaccgtcc aagcgttgca gattgcagtt gatgacgaaa atcaggttgt
     2161 ccaagccttc gcgtgcagcc agtgcgattg caccttgaga ttcgggttcg tccatttcgc
     2221 cgtcgccgca gaaacaccat actttacggc ctttggtttt tgccaaacca cgcgattcca
     2281 agtatttcag gaaacgcgcc tgataaatcg ccatgatggg ccccaagccc atggatacgg
     2341 tcgggaactg ccaaaagtcg ggcaagaggt gggggtgcgg ataggaaggc agaccgtgtc
     2401 cgtccacttc ttggcggaag ttgttcagct ggtcttcggt cagacggccc tcgacgaatg
     2461 cgcgtgcata gatgcccggg gcgacgtgac cttggaagaa gaccaaatcg ccttcttcgc
     2521 cttcgccttt ggctttccaa aagtggttga aaccgacttc gtacatggtg gcggcagatt
     2581 ggaaagatgc gatgtgccca cccagttcca aatctttctt gccggcgcgc aatacgatgg
     2641 cggcggcgtt ccagcgcacg aatgcgcgga tgcggtgttc gatgttttgg tcgcccggaa
     2701 tgcctttttc gttttcaacc gaaacggtat tcaaatacgg ggtggtcgtg ccgtgtggca
     2761 tacgtacgcc cttgtcgcgg cagtatttga ccaggttttc caagaggtat tgcgcgcgtt
     2821 cgccgccttc atattcgagg acggagctta acgcgtccag ccactcttgg gtttcgatag
     2881 ggtcaacatc gtgtaattgg gtggacatag tatctatcct ttatgttgag tgtttaatcg
     2941 gcagggaggg tgtagccaac ccctttattt tcgtttgtga aaaataataa gcaaaaacga
     3001 aaatttctaa atcaaaccga aaaaaataat ttaagtttaa tgttttcaaa aaattataaa
     3061 agttttggaa acaagggaaa acttgaaaaa aatgttcgat ctgctccaca cgaaatccta
     3121 tcagcaatcg ggtctgctgg caaataacca tgcctgtaaa agcaggtttt atactgaagt
     3181 tggcgtttgg taaggtcaag tctttttgat ttcgaaatat tctgtttgat tttggcgata
     3241 ttttttcgga attgtggtat atcgaatcat tatgtctctg tcggcaattt tcctattgta
     3301 gaaaatctat acacaagctt tgtggataac cgtgttgata agccacggct tgtgttaaaa
     3361 aagtcttacg caacaaatgt acgcttattt tgattaaaaa ataggcacat ttaaatctat
     3421 aaaaatcatg tgcttatgtt caatatgatg tttctggtca tgtttttgcc gtctgttatg
     3481 ggatttgtcc cgcgcttggg gcagggggtg aattttggag gttggtgcaa aacctgcttt
     3541 tttcgcgtta caatctgcac ccgatttact tttgaatttg cctgtttgga aaaatacttt
     3601 tatgaaagcc agccaattct ttatctctac tttaaaagaa gcccctgccg aagccgcgct
     3661 tgccagccac aagctgatga ttcgcgccgg tctgattaaa gccaacgcgt ccggccttta
     3721 tacttggatg ccgatggggc tgcgcgtgtt acgcaaagtc gaaaacgtcg tgcgcgagga
     3781 aatggcgcgt gcgggcagcg tggagttgct gatgccggtg gtgcagcctg ccgagctgtg
     3841 gcaggaatcc ggccgctggg agttttacgg taaagagttg ctgcgcctga aagaccgcca
     3901 cgaccgcgat ttctgcatgg gtccgacctg tgaagaagtc atcgccgaca tcgtgcgcaa
     3961 agaaatcaac agctacaaac agctgccgaa aaacttttac cacatccaaa ccaaattccg
     4021 cgacgaagtg cgcccgcgtt tcggcgtgat gcgcgcgcgc gagttcgtca tgaaagacgc
     4081 ttattccttc catgccgact acgcctcgct tcagacgacc tatgatgcca tgtatgacgc
     4141 ttactgccgc atcttcaccc gtctgggctt ggcattccgc cccgtcgccg cagacaccgg
     4201 cagcatcggc ggtacgggtt cgcacgagtt tcaagtgttg gcggaaagcg gcgaagatgt
     4261 gattgcgtac agcgacactt ccgattacgc cgccaatatc gagttggcac cgaccttgcc
     4321 gcttaaaggc gaacgcaccg ctgctcaggc cgagttgacc aaagtacata caccaaacgt
     4381 caaaaccatt gagtctttgg ttgaattcct gaatattccg gttgaacaaa ccctcaaatc
     4441 catcgtggtt gaaggcgaaa acgaaggcga aatcgtctta ttactgttgc gcggcgacca
     4501 tgagttcaac gacatcaagg cagaaaaact cgcaggcgtg agatcgccat tgacgatggc
     4561 aagtcctgcc gcgattgtcg aacagttcgg cgcaaacggc ggctcgctgg gtccggtcag
     4621 ctttgcaggt aaagtttatg ccgattttgc tactgaaaaa ggagcggact gggtcatcgg
     4681 tgcgaacgaa gacggctacc actacaccag cttcaacttc gggcgcgatg ccgccgagcc
     4741 tgaattcgtc gatttgcgta atgtggtcga aggcgacgaa agccccgacg ggcaaggccg
     4801 tctgaaactc gcgcgcggca ttgaagtcgg acacgtcttc cagttgcgcg acaaatacac
     4861 ccaagccatg aacgtaagct tccttgacaa caacggcaaa tcgcaaatta tggaaatggg
     4921 ctgctacggc atcggcatca cccgcgtcgt tgccgccgcc atcgagcaga ataacgacga
     4981 aaaaggcatc atctggacca aagcaatggc gccgtttgaa gtcgttatcg tgccgatgaa
     5041 ctacaaaaaa tcagacaccg tacgcgaagc cgccgacaaa atctatgccg aattgctggc
     5101 ggcaggcgcg gatgtgctgc tggatgaccg cgacgaacgc gcaggcgtat tgctgaacga
     5161 ttccgagctg ttgggtattc cgcaccgcat cgtcatcggc gaccgcgctt tgaaagaagg
     5221 caatgtcgaa tacgccgaac gccgcaacaa cgaagcgcag gcagttgcaa tcggagaaat
     5281 tgttgcgcgt gtaacagctt tattaaatgg gtaagcttgg tcaaatgccg tctgaaaata
     5341 atttcagacg gcatttttat ttggggagaa agtgaagtgg cttcagtatt tttagaagga
     5401 gaagcagtcg aggtcggcaa tattttctgt atcggcagga actatgccgc acatattgaa
     5461 gagttaaaaa acgaaatacc ttccgaacct gtggtgttta tgaagccgtc aggcagcatt
     5521 ttgaacagcg gcggtacaat cttgctgccc gagtttagta gggatgtgca gtttgagtgc
     5581 gaactcgtgc tgctggtcgg taaggattca gacggcacgg gcgagggtaa agatattttg
     5641 ggatgtgtcg caggatatgg tgtagggctg gaccttacgg cacgggatat ccaatgccgt
     5701 ctgaaggaaa aagggctgcc ctggctgaag gcaaaagggt tcaggcattc cgcgtgcgtg
     5761 tcggattttg cggcggcagg cagaatcggc aatcccgaga atgtgttgtt ttcattaaaa
     5821 caaaacggcg tattgaaaca gcgcggggat accggtttga tgatttaccc gattcgggaa
     5881 attttacaca aactggcggc agattacggt ttggggaaag gggatttggt gtttaccgga
     5941 acgccctccg gcgtgggcgc aatcggtgcg ggggacaatt tggcattgga gctggacggc
     6001 ttggtgcgcg cctctttcac ggtagggtgt taaccgcgcc gcttaaccct cacgcccgtt
     6061 gaaacattcc gccggaccgc cctcgaaaaa accgtgcaag atggcttgcg ccgccacttg
     6121 gtcgagcacc gatttgcgtt ttttgccgaa gacctgtgct tccgaaagca ggctttcggc
     6181 atagacggac gacagccgtt cgtcaaccca atagacgggg agattgaacc tgccgttcag
     6241 cctgcgtccg aacttgcgcg acaggtgcgt catttcatgt ttcgtgccgt cggtatgcac
     6301 gggcagtccg acgacaaaat aacgcggctg ccattcttga accagcttgg cgattgccgc
     6361 gaacttttca tcgttgctgc cgccggtaac ggtgctcaaa ggatgggata gccctaattc
     6421 cgcgtctcct tgtgccacgc cgatacgcgc ttcgccgaag tcgaatgcca gtgccgttcc
     6481 ttttggaatt ttatgcatgg ccggctcccg aaaacagggc gagcgggtcg atgccgagtt
     6541 tggcgaatgc ggcggcgtaa cggtgttcgt aggggatgtc gaacaggatg tgttcgtcgg
     6601 cgggaacagt cagccacgca ttgtcggcaa gttcgcgttc gagctggcct tttccccagc
     6661 ttgaatagcc tatgctgatc aaggctttgt caaccgcacc ttcgcgtgaa atattttcaa
     6721 tcacgtctcg ggaagaagtt agcgcgatat tgtctgaaac gccgatactg ctttgccagt
     6781 tgccgatcgg ggtatgcacg acataacctc gctcgacttg caccggcccg cccatcatca
     6841 cgctgtcgtg ctgcatccgc atggggatgt ttttgccggt ggcggaaaaa atcatgtcca
     6901 tcgtaatcgg agagggtttg ttgatggcga tgccgagtgc gccgtcttca tcgtgtttgc
     6961 agatatagac gaccgattgt gaaaaaaacg cgtcttccat atcgggcatg gcgacgagaa
     7021 agtggttgga gaggttcata tcagcgtttg tcgggatttt ctatcgggtt ggaaatcatg
     7081 caggcgccgg caagcaggtt gccggaagta tcggcttgca ccccatcggt ttgcggtatg
     7141 ccgccgccga aacaggaaac cgcgcttaag gaatttttcg gcgagccttg gatgatttta
     7201 tcgctgtaca ccaaataggc gaaggttttg cgtttggggt cgtaataacg gacaatctgc
     7261 cggctcttga acgcgaagct cgcaccgtgt ttgaaaactt ctttcggttt gcgcacggcg
     7321 gtttcgtcaa aagaaatcga agatgccgtc tgaacgcacg aaaccgatgc gtcggacgcg
     7381 tcctcttcca aattgaccat ttccttcaag ccgccttttt ttgcatacga aatataacag
     7441 gcaacccctt gaacgtcggg atcgtcgaat ccttccactt cgatacggtc gtttttgccc
     7501 agtatgttga aaacggtact tgcccgtccg attttatcgg tttcgccgct gccgcaggca
     7561 gtcagcagga cggcggcaga cagcagtagc agtctgttca tcaaaatttc cttatcggtt
     7621 gaatccccgt tttccgtgaa ggcggcaggg cttgctttgt ttggaggggc gcacgttccc
     7681 aaaatcgggc aacatacggt atgcggctta atgtgctacc ctgtgtgtcg gaacacaata
     7741 tccgttgcgg cattgaaaat atgatgcaca atataaggct gtttgagccg attgcaagta
     7801 tccgcgcaaa gaaggcgcaa gattgggcaa acggcatttt cagacggcat aactgacagt
     7861 ataatccaaa cgtccggctg ctttcgtgca gccttcagta ttatccaact ttttccgaag
     7921 gtcgaaccat gcgctacaaa ccccttctgc ttgccctgat gctcgttttt tccacgcccg
     7981 ccgttgccgc ccacgacgcg gcacacaacc gttccgccga agtgaaaaaa cagacgaaga
     8041 acaaaaaaga acagcccgaa gcggcggaag gcaaaaaaga aaaaggcaaa aatggcgcag
     8101 tgaaagataa aaaaacaggc ggcaaagagg cggcaaaaga gggcaaagag tccaaaaaaa
     8161 ccgccaaaaa ccgcaaagaa gcagagaagg aggcgacatc caggcagtct gtgcgcaaag
     8221 gacacgaagg ggataagaaa tcgaaggcgg aacacaaaaa ggcacatggc aagcccgtgt
     8281 ccggatccaa agaaaaaaac gcaaaaacac agcctgaaaa caagcaaggc aaaaaagagg
     8341 caaaaggaca gggcaatccg cgcaagggcg gcaaggcgga aaaagacact gtttctgcaa
     8401 ataaaaaagt ccgttccgac aagaacggca aagcagtgaa acaggacaaa aaatacaggg
     8461 aagagaaaaa tgccaaaacc gattccgacg aattgaaagc cgccgttgcc gctgccacca
     8521 atgatgtcga aaacaaaaaa gccctgctca aacaaagcga aggaatgctg cttcatgtca
     8581 gcaattccct caaacagctt caggaagagc gtatccgcca agaacgtatc cgccaagagc
     8641 gtatccgtca ggcgcgcggc aaccttgctt ccgtcaaccg caaacagcgc gaggcttggg
     8701 acaagttcca aaaactcaat accgagctga accgtttgaa aacggaagtc gccgctacga
     8761 aagcgcagat ttcccgtttc gtatcgggga actataaaaa cagccagccg aatgcggttg
     8821 ccctgttcct gaaaaacgcc gaaccgggtc agaaaaaccg ctttttgcgt tatacgcgtt
     8881 atgtaaacgc ctccaatcgg gaagttgtca aggatttgga aaaacagcag aaggctttgg
     8941 cggt
//
LOCUS       DAWHSK010000056         8843 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.056, whole genome shotgun sequence.
ACCESSION   DAWHSK010000056 DAWHSK010000000
VERSION     DAWHSK010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8843
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.056"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..320
                     /locus_tag="WIC03_000860"
     CDS             <1..320
                     /locus_tag="WIC03_000860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883388.1"
                     /translation="LKNIPLKDFDEVCEKWEKSFKENGKEIEYNQKYSRIYGEVRGAG
                     FNGDIGEKLHRCAVMEWAENETVKLAQKWEQEQKKQQIQQKKETEKSPKHKASRDDWE
                     MER"
     gene            complement(402..2489)
                     /locus_tag="WIC03_000861"
     CDS             complement(402..2489)
                     /locus_tag="WIC03_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003700725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HEZ3883389.1"
                     /translation="MDYLSRLSFGFNKKLPVILQTEVAECGLACLTSILSYYGFHTDL
                     RTLRQKYTLSLKGANLADIMRFGNEMNLTPRALRLELDELSNLQLPCILHWNLNHFVV
                     LCSISKDSIVIMDPAVGMRKIKMDEVSQKFTGIALELFPNTHFEEKKETKKIKILSLL
                     RGISGLKRSLIQMLILAISLEVFALVSPFFMQWVIDHVIVTADKNLLLTLTLGFGLLA
                     ILQQLISLLQAWVGMHLSTTLNLQWKANIFKRLLDLPNDYFSKRHLGDVISRFGSIDH
                     IQETLTSTFFVLVLNSLMAVFTFVLMTIYSTQLSLIVLLTLVLYILIRWLAYYPLRNA
                     TEENIVHEAKQNSYFMETIRGIQSVKLFDKHYQRHGTWMSLFVNTVNTKLTTDKLSAL
                     FEFSNKLLFSMENIIIIYLGASAILDGSFTVGVLMAFLAYKGQFESRTASLVDQYIQI
                     KMLGLHAERLADITLNETETEIIKYNHIPKLDNEQLVLKVENVSFRYADNEPYLFENI
                     NLEFKDNEAVVLTGQSGRGKSTLLNILTGSLKPETGTVSINGHDIYQVSPSFIRGLSG
                     IVRQDDVLFAGSIGENISFFDESPNMELIEQCAKMAQIHDDILKMPMGYETLIGDMGN
                     ILSGGQKQRVILARALYKRPKILFLDEASSHLDVENEQKINHNLKSLGIMKIMVAHRQ
                     ETIQSADKILNLG"
     gene            complement(2492..3757)
                     /locus_tag="WIC03_000862"
     CDS             complement(2492..3757)
                     /locus_tag="WIC03_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEZ3883390.1"
                     /translation="MSHLFRKEVFVAQQNKWTGQVILTRPFSFLFLTFCAFLIALCII
                     IFLIFGSYTNKTTVEGQLLPTMGVVRVYSSDIGTITHKFVEDGNFVKAGEPLFKLSTS
                     RFGEKGNVQAKLAAEANLKKTLALQELERLKRIHQNEQKNVHNNIHRLNNQLENIKQQ
                     ITGQNRQIRLAEKTLNKNKFLASQGAVSQQDKMTAESHLLEQRSRLESLKLEQNNAIR
                     ELDEQKITLSSLPERHKTELSQLNRAITEMNQEILDFDLKSEQTIRASKSGYISTINV
                     DIGQQVEPSKLLLSIVPEQTELVANLYIPSKAVGFIKPKDKVVLRYQAYPYQKFGHAT
                     GEIISVARTALGKQELSGLGIIFTNPTLLNEPAYLVKVKLEKQTIKAYGENKPLQIGM
                     ILEADILHERKKLYEWVLDPLYSISGKIN"
     gene            complement(3724..3938)
                     /locus_tag="WIC03_000863"
                     /pseudo
     CDS             complement(3724..3938)
                     /locus_tag="WIC03_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235355.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4978..5214)
                     /locus_tag="WIC03_000864"
     CDS             complement(4978..5214)
                     /locus_tag="WIC03_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin transporter"
                     /protein_id="HEZ3883391.1"
                     /translation="MKELHTSELVEVSGGKFHIFAQGGGNLGKKDMVAVGKIGASYSP
                     NNSGVEFSVSKQFGYVQGLGVQFSKPTFGISKKW"
     gene            complement(6268..6939)
                     /locus_tag="WIC03_000865"
                     /pseudo
     CDS             complement(6268..6939)
                     /locus_tag="WIC03_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557955.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            7200..8672
                     /gene="pyk"
                     /locus_tag="WIC03_000866"
     CDS             7200..8672
                     /gene="pyk"
                     /locus_tag="WIC03_000866"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEZ3883392.1"
                     /translation="MNQTSRDLTRISHNTKIVATLGPGSNNVELLEDMIRVGGLNVVR
                     FNFSHGTPEFHQENARIVREAAKRAGQEIAIIADLQGPKIRVGKIAGGGIELNKGETL
                     VLDAALEGEGTREAVGLDYRDLPDDVTAGDVLWLDDGLLTLTVESVEGSRIITRVENS
                     HVLKSNKGINKRGGGLSAGALTEKDFRDLKTAIAIGCDYLAISFVKSAEDLHIARAKV
                     EEEMKGSTAVRPGLVSKIERVEAIENLDEIILAGDGIMVARGDLAVEVGHAAVPALQK
                     RMIRRARELRRFSITATQMMESMITNPVPTRAEVSDVANAVLDGTDAVMCSAETAVGA
                     YPFETVSQMAIICAAAEKEQDSLNGVAEQAEYPEAVSTNLAVAGGAVSVARAVHAKAI
                     VALTESGSTAFEISRHNITLPIFALTPSVSAQRRMAMYRGVRPLILATSTDHDTALNE
                     VETMLVEHNILHSGDQYIITSGSQMRESGSTNTLEVLRVK"
BASE COUNT         2699 a         1784 c         1716 g         2644 t
ORIGIN      
        1 ccttaaaaaa tatccctttg aaagattttg atgaagtttg tgaaaagtgg gagaaatcct
       61 ttaaagaaaa tggaaaagaa attgaatata atcaaaaata ttcaagaatt tatggtgaag
      121 ttcgtggagc tggctttaat ggcgatatcg gagaaaagct acatcgttgt gccgtgatgg
      181 aatgggctga aaatgagacc gtgaaattag cccaaaaatg ggaacaagag cagaaaaaac
      241 aacaaattca acagaaaaag gaaactgaaa aatcgccaaa acacaaagcc agtcgtgatg
      301 attgggaaat ggaacgttaa accgctattt tcatgtattc tattaaatta tttgaaaaaa
      361 gacttcactt tttgttaaag aaaaatgaag tcttgttctg ttcaacctaa attcagaatt
      421 ttatctgccg attgaattgt ttcttggcgg tgtgcaacca ttattttcat aataccaaga
      481 ctttttaggt tatggttaat tttttgttca ttttctacat ctaaatggct acttgcttcg
      541 tctaaaaata gaattttggg tcgtttatac aatgcacgag ccaagataac tctctgcttt
      601 tgtccacctg ataagatatt tcccatatcg ccaatcaagg tctcatagcc cattggcatt
      661 ttaagtatat cgtcatgtat ttgtgccatt tttgcacatt gttcaatgag ctccatattt
      721 gggctttcat caaaaaatga aatattttcc ccaatagaac ctgcaaaaag gacatcatct
      781 tggcgaacaa tcccgctcaa tcccctaata aaggatggag aaacttgata tatatcatgc
      841 ccattaatac taactgtacc agtttcaggt tttaggctac ctgttaaaat gtttaacaaa
      901 gtggacttcc cccgaccaga ttgtcctgtt aaaacaactg cttcattatc tttaaattcc
      961 aaattaatgt tttcaaaaag atatggctca ttatcagcat atctgaatga gacgttttca
     1021 actttaagaa ccagttgttc attatctaat ttaggtatat gattatactt aataatttca
     1081 gtttctgttt catttaaagt aatgtcagcc aaacgttcag catgaagccc taacattttg
     1141 atttggatgt attggtcaac gagagaagct gttctgcttt caaattgccc tttataagcc
     1201 aaaaaagcca tcagaacacc gactgtaaat gaaccatcta aaattgcgct tgcaccaaga
     1261 taaattatga taatattttc catgctaaac aacagtttat ttgaaaattc aaataaagca
     1321 gagagtttat ctgttgtcag cttggtattg actgtattca caaataggct catccaagtg
     1381 ccatgtcttt gataatgttt atcaaataat ttaactgatt ggataccacg aatggtttcc
     1441 atgaaatatg agttttgttt ggcttcatga acaatatttt cttctgttgc atttcttaat
     1501 gggtaatatg caagccaacg aattagtatg tacaaaacaa gtgttaaaag aacaatcagc
     1561 gataattgag tgctgtaaat tgtcattaac acgaaagtaa aaacagccat taagctattt
     1621 aaaactaaaa caaaaaaagt agaagttagt gtttcttgga tatgatctat tgaaccaaat
     1681 cttgaaatca catctcctaa atgtcgttta ctgaaatagt cattaggtaa gtcaagtaac
     1741 cttttaaata tattggcttt ccattgtaaa ttaagagttg tagataggtg catacctacc
     1801 catgcttgta acaggctaat taactgttgc aggatagcca gtaaaccaaa tcccaaagta
     1861 agggtcagta ataaattttt atcagcagtt acaatgacat ggtctattac ccattgcata
     1921 aagaatggac taaccaatgc aaagacttcc aaagaaatag ctaatataag catttgaatt
     1981 aaagagcgtt ttaagcctga aatccccctt aatagagata atattttgat tttctttgtt
     2041 tcttttttct cttcaaaatg ggtattgggg aataattcta gggcaatccc tgtgaatttt
     2101 tgtgaaactt cgtccatttt gatttttcgc ataccgacag cagggtccat aatgacgata
     2161 ctgtctttgg aaatggaaca aagtacaaca aaatggttta agttccaatg gagaatgcag
     2221 ggtagttgta aatttgacag ctcatctaac tctaaacgca aagctcgtgg cgttaaattc
     2281 atttcattgc caaatctcat gatgtctgca agatttgcgc cctttaatga cagggtgtat
     2341 ttttggcgta acgttcttaa atcagtgtga aagccataat aggacaagat ggatgtcagg
     2401 catgctaaac cacattcagc aacttctgtt tgcagaatga caggtagctt tttgttaaat
     2461 ccaaaggaca gtcttgataa ataatccatt tttaattgat ttttcctgaa atgctgtaaa
     2521 gtgggtcaag tacccattcg tacaattttt ttcgttcatg gagaatatct gcttctaaaa
     2581 tcatgccaat ttgaagcggc ttgttttctc cgtatgcttt aatcgtttgt ttttccaatt
     2641 taactttcac aagataggca ggttcattta ataaggttgg gttagtgaaa atgataccta
     2701 aacctgatag ctcttgttta ccgagagcag ttctggcaac tgaaataatt tctcctgtgg
     2761 catgtccaaa tttttggtaa gggtacgctt ggtaacgtaa aacaacttta tctttcggtt
     2821 taataaaacc aacagcttta ctgggtatgt aaagattggc gaccaattca gtttgttcag
     2881 ggacaatgct taacagcaat ttagacggtt caacttgttg ccctatatca acattaattg
     2941 ttgatatata acctgattta ctagctcgta tggtttgttc ggatttcaaa tcaaaatcca
     3001 aaatttcttg gttcatttcc gtaatcgcac ggttgagttg gctcaattcg gttttatggc
     3061 gttcaggcag gctgcttaat gtaattttct gttcatcaag ttccctgatt gcattatttt
     3121 gttcaagttt taggctctcc aaacgtgagc gttgttccaa taaatggctt tcggcggtca
     3181 tcttatcttg ttgggatact gcgccttgac tggctaaaaa cttgttcttg ttaagggttt
     3241 tttccgctaa acgaatttga cgattttgcc ctgtaatttg ctgtttaata ttctctaatt
     3301 gattgtttaa acgatgaatg ttgttatgaa catttttttg ctcattttga tgaatgcgct
     3361 ttaaacgttc caattcttgt aatgccaaag tttttttaag gttggcttct gctgccaatt
     3421 tggcttgtac gtttcctttt tcgccaaaac gcgatgtgga aagtttgaac aatggttcgc
     3481 cagctttgac aaagttacca tcttcaacaa atttatgcgt aatcgtgccg atatcggaag
     3541 agtaaacacg aaccaccccc atagttggaa gtaattgacc ttcaacggtt gttttattgg
     3601 tatagctacc aaaaatcaaa aagataatga tacacagagc aatgagaaaa gcgcaaaaag
     3661 tcagaaataa aaaagagaat ggacgggtca agataacctg acctgtccac ttattttgtt
     3721 gggctacaaa gacttctttt cggaataagt gggacattta ttattatttc ttaaaaaaat
     3781 ggatagaaaa acaccaataa ttaaatactt atgcctaata acataaattt gctattgatt
     3841 gatgtttcta ttttaattac attaaattta accaataatc cagaaatggt tgcagctaaa
     3901 atgcaaaaga cagtggcgta aatggtttca gtattcataa tttttctctt tcaaaattgt
     3961 tcaatctaaa tagaaaaagg taacttcaat acatattgct gtaactgaaa ttaccttttc
     4021 cttaatgatg taattatctt atggcttcat catgccacga ccgatattgt atccgccagc
     4081 agcagagaaa ataccagctc caattgcgcc aacccagcct actggtcctg tccacttgtt
     4141 ccgttgagct acaaagactt ctggtctgaa aaatgagtga ttgttagaca tgtgtactta
     4201 tttatgtttc tttgattggg ataaatttgt aaaaacaaaa aaaaggcaag ttgttattat
     4261 agaagaactt aaaatgctta acatactcat tgaggtttgt accacataat gagatacgag
     4321 ataatttgtt gggagtaaaa tcaaaaaaag attaaatatt taaaataatc actatatttt
     4381 ttcattattt gttccttttt gtatttaagt tcttatgaaa aactagggta ttttgaatag
     4441 aaaatatcaa aataccctag ttttattaag gaatattacc accaacaagt gcttccatat
     4501 tgtacagcac cagtgattgc accaccaata gctccgaatt tcgcacttgg aatagcacca
     4561 ataccacctg caaatgaacc aacaattgcc ccaccagctg ctccacctaa tgcactacca
     4621 atggtatttt ttgagaaatc acgccagtta caagcagcac cagaaacttg ttaaatttca
     4681 ttcaaagtca aaacttgcat aatattcacc tttgttaagt gtggttgaga aaaaagtaaa
     4741 ttttcatcgt tactcattat ttgcaatcat gcatatactg taaccaagca ctatattcag
     4801 ttggatcata gtttttccta ctgggttgtc tgatatgact attggcacta ttggcacaat
     4861 tgggattagc aactttattg tgagtaccta atggattgat atttcctgta aagtggctat
     4921 tgcatgcgct taatgagcat agatttatta atgtcagaaa ggataaaaca aaaaatctta
     4981 ccatttttta ctaataccaa aagtaggttt cgaaaactgt acaccaagac cttgtacata
     5041 tccaaattgc ttgctaacag aaaactctac tccactattg ttaggggaat aggaagcacc
     5101 aattttacca acagcaacca tatctttttt acctaggttg ccgccaccct gtgcaaagat
     5161 atggaatttg ccacctgaca cttcaactaa ttcagaggtg tgtaactctt tcatgatttt
     5221 ctcctgtaaa gaagtatggt tggaaatgta aatcagaatt aacctttctt aagtttaatt
     5281 taatactttt tttgcaagta ttttatttta aattaaatca atattttaaa aatatttact
     5341 tgtatactga atatttactt gtatactgaa aaagaaactc ctaatggtgg agtggggctt
     5401 ttgtttaagt aagcattgaa aaaatttaaa tttcccatta tgattaactt aatgcaagag
     5461 ctttttcagg acgtacaaac cagcgaacgg gcgttctgat gtgggcaggt tggttgtcgc
     5521 taaccaattt cagtagctca atcgtcagat tggtatccgg ccctatcaag ctaaggagag
     5581 gctgcggcta ctacacaaag cacctgaaat tattgggtgc ttttgtcttt ttatggcatt
     5641 gtaccaattt gtgtaccacg agggcttgtt cgaccaaacg cggtaacaac tgaatacaca
     5701 ctttccccct caacttcttt gcgttctaaa acgactgtcc caatccgaga atagacaatc
     5761 ataggacgat gattgcccct tacatcacta aattcactaa aaatttttgc gcctgattga
     5821 aaaatcattt ccacataaaa gggttacacc tgtttcatcg tggcttaatc caaaccgcgt
     5881 taaatctgct ttgtgttctg ccaaaatgtg aatcacacca aagccgtgat taaccttttt
     5941 gtgttcgccg tgttttaaaa caatttgacc tgccgggaag tctgctctat tgttcggaca
     6001 atagggggaa tataccaatt actttaacta catcattttt tgacattttt cttgcctttc
     6061 aatttaaaat ccaaaatcat actgccataa tttagcaatc caaaaaaatt taggcagcag
     6121 tcatggtttc gtattttaga aaacgaaact tggtttggtg ttttgaagca tttcagtaca
     6181 caaacatgga attttaaatt gtcttgtgtt gccttgattt aggatgttcc agttttttaa
     6241 taaatagttt ggggctgtcc tagataacta ggacaaactt gattttacta attgttttaa
     6301 aatggaacaa gaacttttat ctcactgttg ttaaaacgtc gttcgcactc ctttaaatac
     6361 agctcaaaat gcgctttggg aatgccgtta aacttgcgta aatgacgttt tgcctggttc
     6421 caaaagttct caattccatt aatatggttt tgtcgttcgg caaaatgtgt gctgtgattg
     6481 atacgaaaac gaagtttcag cgaagctaaa atggctaaat tcgctcacat ctaattacat
     6541 catagctacg ataacaatcc gtataaacaa tactgtcagg tttcacttgt tcacggataa
     6601 taggaaataa agtagcggtt tgagtattcg gtactgtaac cgtataaacc ttaccatttc
     6661 gcttcaaaag accgaatacg gcgacttacc ggcagcaccg cgaccgcgtt tgcctttgcg
     6721 ttgtccgcca aaataacttt catctgcttc tacttcgcca tcaaacattt ccaaatgcgg
     6781 actgttttga taaataagta atcgtaaacg atgaaaataa taggctgcgg tatttttatt
     6841 aacgcctact aactctgctg ccgttcttgc agttacacct gcgacaaaca gttcaatgag
     6901 tttattttgt ttataccggc ttagacgact ttttctcata gggataattc taacttaatt
     6961 tgaatttccc tagttatcta ggacagctcc aaaaataaat ttagcctaag tcataaaacc
     7021 gtgtaatttt gcaccaaaaa agacggtttg caggtaaaat atcaaactat taatttaaat
     7081 catattttta cagaatattc cgccgcattc atcaaatgga catcaaaccg gttccaaatt
     7141 tctgtaattt tgcaacaaaa taccgcaaaa cacccgattg agaccgaaag gactttcata
     7201 tgaaccagac aagccgcgat ctgacccgca tcagccacaa caccaaaatc gtcgccacct
     7261 tggggccggg cagcaacaac gtcgaactgt tggaagacat gatccgcgtc ggcggtctga
     7321 acgtcgtccg cttcaacttc agccacggca cgcccgaatt ccatcaggaa aacgcccgca
     7381 ttgtgcgcga ggcggcaaaa cgcgccggac aggaaatcgc catcattgcc gacctgcaag
     7441 ggccgaaaat ccgcgtgggc aaaatcgccg gcggcggcat cgaattgaac aaaggcgaaa
     7501 cgctggtact ggatgccgcg ctcgaaggcg aaggcacgcg cgaggcggtc ggtttggact
     7561 accgcgacct gcccgacgac gttaccgcag gcgacgtatt gtggctggac gacggcctgc
     7621 tgaccctgac cgtggaatcc gtcgaaggca gcaggattat cacaagggtg gaaaacagcc
     7681 atgtcctgaa aagcaacaaa ggcatcaaca aacgcggcgg cgggctgtcc gcaggcgcgt
     7741 tgaccgaaaa agacttccgc gacctgaaaa ccgcgattgc catcggttgc gactacctcg
     7801 ccatcagctt tgtgaaatct gccgaagatt tgcacatcgc gcgcgccaaa gtcgaagaag
     7861 aaatgaaggg cagcactgcc gtgcgccccg gtttggtttc caaaatcgaa cgcgtagaag
     7921 cgattgaaaa ccttgatgaa atcatcctcg ccggcgacgg cattatggtt gcgcgcggcg
     7981 acttggcggt cgaagtcgga cacgccgccg tccccgccct gcaaaaacgg atgatccgcc
     8041 gcgcccgcga gttgcgccgc ttcagcatta cggcgacgca aatgatggaa tcgatgatta
     8101 ccaaccccgt gccgacccgc gcggaagtca gcgatgtggc aaacgcggta ttggacggta
     8161 ccgatgcggt gatgtgttcc gccgaaaccg ccgtcggcgc gtatccgttt gaaaccgtca
     8221 gccaaatggc aattatctgc gcggctgcgg aaaaagagca ggattcgctc aacggcgttg
     8281 ccgaacaggc ggagtatccc gaagcggtca gcaccaacct ggcggttgcc ggcggcgcgg
     8341 tcagcgtggc gcgcgcggtt cacgccaaag cgattgtcgc cctgaccgaa agcggttcga
     8401 ccgcctttga aatcagccgc cacaacatca ccctgccgat tttcgcgctg accccgagcg
     8461 tttccgccca acgccgtatg gcgatgtacc gcggcgtgcg cccgctgatt ttggcaacca
     8521 gtaccgacca cgacacggcg ttgaacgaag tcgaaaccat gctggtggaa cacaacatcc
     8581 tgcattccgg cgaccaatac atcatcacca gcggctcgca aatgcgcgaa tccggttcga
     8641 ccaatacgct ggaagtgctg cgcgtcaaat aatccgccct gagtggaaaa tgccgtctga
     8701 agtcgatgcc cgaggcttca gacggcattt tttgcggcgg cgcggcggtt caggcaaact
     8761 gaaaaaattg tgcaaatccg gcaacatcag ataaaattga gtacctatac taaagcgaaa
     8821 caaggcattt ccgactgcct ttt
//
LOCUS       DAWHSK010000057         8806 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.057, whole genome shotgun sequence.
ACCESSION   DAWHSK010000057 DAWHSK010000000
VERSION     DAWHSK010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8806
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.057"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..297)
                     /locus_tag="WIC03_000867"
     CDS             complement(<1..297)
                     /locus_tag="WIC03_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ3883393.1"
                     /translation="MNILLLDGGKAFGHSHGGLNRTLHKKAKEVLTALGHNVQETVID
                     AGYDVEAEIEKFVWMDAVIWQMPGWWMHEPWTVKKYIDEVLTAGHGKLYQSDGRH"
     gene            536..1435
                     /gene="crgA"
                     /locus_tag="WIC03_000868"
     CDS             536..1435
                     /gene="crgA"
                     /locus_tag="WIC03_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151940473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator CrgA"
                     /protein_id="HEZ3883394.1"
                     /translation="MKTNSEELTVFVQVVESGSFSRAAEQLAMANSAVSRIVKRLEEK
                     LGVNLLNRTTRQLSLTEEGAQYFRRAQRILQEMAAAETEMLAVHEIPQGVLRVDSAMP
                     MVLHLLAPLAAKFNERYPYIRLSLVSSEGYINLIERKVDIALRAGELDDSGLRARHLF
                     DSRFRVIASPEYLAKHGTPQSAEELAGHQCLGFTEPGSLNTWAVLDAQGNPYKISPHF
                     TASSGEILRSLCLSGCGIACLSDFLVDNDIAEGKLIPLLAEQTSNKTHPFNAVYYSDK
                     AVNLRLRVFLDFLVEELGNNLCG"
     gene            complement(1662..4877)
                     /gene="carB"
                     /locus_tag="WIC03_000869"
     CDS             complement(1662..4877)
                     /gene="carB"
                     /locus_tag="WIC03_000869"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEZ3883395.1"
                     /translation="MPKRTDLKSILIIGAGPIVIGQACEFDYSGAQACKALREEGYKV
                     ILVNSNPATIMTDPEMADVTYIEPIMWQTVEKIIAKERPDAILPTMGGQTALNCALDL
                     ARNGVLAKYNVELIGATEDAIDKAEDRGRFKEAMEKIGLSCPKSFVCHTMNEALAAQE
                     QVGFPTLIRPSFTMGGSGGGIAYNKDEFLAICERGFDASPTHELLIEQSVLGWKEYEM
                     EVVRDKNDNCIIICSIENFDPMGVHTGDSITVAPAQTLTDKEYQIMRNASLAVLREIG
                     VDTGGSNVQFAVNPENGEMIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGFTLDE
                     LRNDITGGKTPASFEPSIDYVVTKIPRFAFEKFPAADDRLTTQMKSVGEVMAMGRTIQ
                     ESFQKALRGLETGLCGFNPRSSDKAEIRRELANPGPERMLFVADAFRAGFTPEEIHEI
                     CAIDPWFLAQIEDLVKEEKSVSDGILSDLDFAALRRLKRKGFSDKRIAQLLGVKEKEV
                     REHRYALKLHPVYKRVDTCAAEFATETAYLYSTYEEECESRPSDRKKVMILGGGPNRI
                     GQGIEFDYCCVHAALALRESGFETIMVNCNPETVSTDFDTSDRLYFEPLTLEDVLEIV
                     RTENPWGVIVHYGGQTPLKLANALVENGVNIIGTSADSIDAAEDRERFQKVLNDLGLR
                     QPPNRIAHNEEEALVKAEEIGYPLVVRPSYVLGGRAMQIVHSAEALQKYMREAVQVSE
                     DSPVLLDFFLNNAIEVDVDCVSDGKDVVIGGIMQHVEQAGIHSGDSGCSLPPYSLSEE
                     IQDEIRRQTKAMAYALGVVGLMNVQFAVQDGVVFVLEVNPRASRTVPFVSKATGVPLA
                     KVGARCMAGISLKEQGVEKEVVPDFYAVKEAVFPFIKFPGVDTILGPEMRSTGEVMGV
                     GASFGEAYYKAQLGAGERLNPTGKIFLSVREEDKERVIKTAKNFQALGYGICATRGTA
                     QYLTEHGLIVQTINKVPEGRPHIGDALKNGEIALVVNTVSSDPQSVSDSHIIRQSALQ
                     QRVPQYTTTAGGEAMSEGAKSRDHLGVYSVQELHGRLKNRS"
     gene            complement(4906..5547)
                     /locus_tag="WIC03_000870"
     CDS             complement(4906..5547)
                     /locus_tag="WIC03_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883396.1"
                     /translation="MSIPINFNNLKYLLNDMRNKNRIIEAFPFNYNQRQYAVILTRYK
                     PDEPRPDDYAQAKLEFFNLNSENSIFAYADFYEVHFKSATDFINFFKINVQAGAAKIR
                     EIFQSFSNLFADFIPTQTKKDLDIIYKKIVATRLEPNSPNTIYCYDVRRNGKDKAGKP
                     NRRSVENSEKAKILRPELYEKFKADSNYSFFFSDNPSDEKTDAEIIREVTNRQ"
     gene            5709..6107
                     /locus_tag="WIC03_000871"
     CDS             5709..6107
                     /locus_tag="WIC03_000871"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883397.1"
                     /translation="MRYVCTRSPFSLQPQPTACVPCGTLSADEFKGRLKKRSQRLFHL
                     PCDKGCLKHSRCRCFGHSFSDDLQRLNLLQPAPSPVGEGWGEGIPRISATFPNTLTAK
                     TQALWLVALSLTLSHGREDWAVVGAKVSVN"
     gene            complement(6215..6604)
                     /locus_tag="WIC03_000872"
     CDS             complement(6215..6604)
                     /locus_tag="WIC03_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEZ3883398.1"
                     /translation="MNPHEKLLTTDNPILRQRAKAMRQEMSGAEAKLWQHLRAGRLNG
                     YKFRRQQPMGNYIVDFMCVTPKLIVEADGGQHTEQAAYDHARTAYLNSLGFTVLRFWN
                     HEILQQTNDVLTEILLVLQELEKQAAR"
     gene            complement(7177..7302)
                     /locus_tag="WIC03_000873"
                     /pseudo
     CDS             complement(7177..7302)
                     /locus_tag="WIC03_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687282.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(7348..8481)
                     /gene="carA"
                     /locus_tag="WIC03_000874"
     CDS             complement(7348..8481)
                     /gene="carA"
                     /locus_tag="WIC03_000874"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEZ3883399.1"
                     /translation="MSTPALLVLADGSVFHGTSIGYEGSTSGEVVFNTSMTGYQEILT
                     DPSYCKQIVTLTYPHIGNTGTNAEDEESRSVYAAGLIIRDLPLLHSNFRASESLHDYL
                     VRNKTVAIADIDTRRLTTLLREKGAQGGAILTGADATVEKAQELIAAFGSMVGKDLAK
                     EVSCTETYEWTEGEWELGKGFVTPDEQPYHVVAYDFGVKTNILRMLASRGCRLTVVPA
                     QTSAQDVLALNPDGVFLSNGPGDPEPCTYAIEAVQKLMESGKPIFGICLGHQLISLAI
                     GAKTLKMRFSHHGANHPVQDLDSGKVVITSQNHGFAVDADTLPANARITHKSLFDNTL
                     QGIELTDKPVFCFQGHPEASPGPQDVGYLFDKFIGNMKAAKRA"
BASE COUNT         1953 a         2248 c         2305 g         2300 t
ORIGIN      
        1 gtgtctgccg tcgctttggt agagtttgcc gtgtccagcg gttaatactt cgtctatgta
       61 ttttttcact gtccaaggct cgtgcatcca ccagcccggc atctgccaaa tcacagcatc
      121 catccaaacg aatttttcga tttctgcctc aacatcatag ccggcatcaa tcacggtttc
      181 ttgaacattg tgtccgagcg cggtcaaaac ttctttcgct tttttgtgaa gcgtgcggtt
      241 taacccgccg tgagaatgtc cgaacgcttt gccgccgtct aataataaaa tattcatttg
      301 ttacctcgtt tgtgaattga tgaatgtatt gtgccgtttt actttataat ttaaaagtgc
      361 aaaaataaga aaacactttt gcgtcaaatg aaataatcag atgttccaac acacgggatg
      421 gcacataaag caccgccctg tgtgccgtcc tgattcggaa ggggttacac ccctcccaaa
      481 taaagtctga tcctgccgcc ctaaagggcg gggttccaac cgaaaaggaa acacgatgaa
      541 aaccaattca gaagaactga ccgtatttgt tcaagtggtg gaaagcggca gcttcagccg
      601 tgcggcggag cagttggcga tggcaaattc tgccgtaagc cgcatcgtca aacggctgga
      661 ggaaaagttg ggtgtgaacc tgctcaaccg caccacgcgg caactcagtc tgacggaaga
      721 aggcgcacaa tatttccgcc gcgcgcagag aatcctgcaa gaaatggcag cggcggaaac
      781 cgaaatgctg gcagtgcacg aaataccgca aggcgtgttg cgcgtggatt ccgcgatgcc
      841 gatggtgctg catctgctgg cgccgctggc agcaaaattc aacgaacgct atccgtatat
      901 ccgactttcg ctcgtttctt ccgaaggcta tatcaatctg attgaacgca aagtcgatat
      961 tgccttacgg gccggagaat tggacgattc cgggctgcgt gcacgccatc tgtttgacag
     1021 ccgcttccgc gtaatcgcca gtcctgaata cctggcaaaa cacggcacgc cgcaatctgc
     1081 agaagagctt gccggtcacc aatgtttagg cttcaccgaa cccggttctc taaatacatg
     1141 ggcggtttta gatgcgcagg gaaatcccta taagatttca ccgcacttta ccgccagcag
     1201 cggtgaaatc ttacgctcgt tgtgcctttc aggttgcggt attgcttgct tatcagattt
     1261 tttggttgac aacgacatcg ctgaaggaaa gttaattccc ctgctcgccg aacaaacctc
     1321 caataaaacg caccccttta atgctgttta ttacagcgat aaagccgtca acctccgctt
     1381 gcgcgtattt ttggattttt tagtggagga actgggaaac aatctctgtg gataattcta
     1441 taaaaacctg ccataaaatt gtttttactc acttaataac tttgagaaaa aataaataag
     1501 atgatgtata gtcggcggca atggcagtct atatggggag cgtgataggg ctgttcctta
     1561 tgtacggctt gtgtgtggca acatcggctt tccaaatgac aaaatgccgt ctgacaccgt
     1621 aaaaaaaggt ttcagacggc attttcaacc cgattcaggc atcagctgcg gtttttcaaa
     1681 cgcccgtgca gttcttgaac gctgtacacg cccaagtggt cgcggctttt cgcgccttcg
     1741 ctcatcgctt cgccgcccgc agtagtggtg tattgcggca cacgttgctg caatgcgctt
     1801 tggcggatga tgtggctgtc ggacacggat tgcggatcgc tggaaacggt attcacgacc
     1861 agtgcgattt cgccgttttt cagcgcgtcg ccgatgtgcg ggcggccttc ggggacttta
     1921 ttgatggttt gtacaatcag cccgtgttcg gtcaggtatt gcgccgtgcc gcgcgtggcg
     1981 cagatgccgt agcctaaggc ttggaagttt ttggcggttt taatgacgcg ttctttgtct
     2041 tcttcgcgca cggagaggaa gattttgccg gtcgggttga ggcgttcgcc cgcgccgagt
     2101 tgggctttgt agtaggcttc gccaaagctt gcgcccacgc ccatgacttc gccggtggag
     2161 cgcatttccg gtcccaaaat cgtatccacg cccgggaatt tgatgaatgg gaacacggct
     2221 tctttaacgg catagaaatc ggggacaact tctttttcca cgccttgttc tttcagggaa
     2281 atgcctgcca tgcagcgcgc gccgactttg gcgagcggca cgccggtggc tttggagacg
     2341 aagggcacgg tgcggctggc gcgcgggttc acttccaata cgaacaccac gccgtcttgt
     2401 acggcaaact gcacgttcat cagtccgacc acgcccagcg cgtacgccat cgctttagtt
     2461 tggcggcgga tttcgtcttg gatttcttcg cttaaggagt agggcggcag cgagcagccg
     2521 gagtcgccgg agtggatgcc cgcctgttcg acgtgctgca tgatgccgcc gataaccacg
     2581 tctttgccgt ctgaaacgca gtccacatcc acttcaatcg cgttgttgag gaagaaatcg
     2641 agcaacacgg ggctgtcttc ggaaacctgc acggcttcgc gcatgtattt ttgcaaggct
     2701 tcggcagagt ggacaatctg catcgcgcgt ccgcccaaaa cataagacgg gcgcacgacc
     2761 agcggatagc cgatttcttc ggctttgacg agtgcttctt cttcgttgtg ggcgatgcgg
     2821 ttgggcggtt ggcgcagacc taagtcgttc aacacttttt ggaagcgttc gcggtcttcg
     2881 gcggcgtcga tgctgtcggc ggacgtgccg atgatgttta cgccgttttc aaccagcgcg
     2941 ttggcgagtt tgagcggggt ttgaccgccg taatgcacaa tcacgcccca cgggttttcg
     3001 gtgcggacga tttccaacac gtcttccaat gtcagcggct cgaaatagag gcggtcgctg
     3061 gtgtcgaagt cggtggacac ggtttctggg ttgcagttga ccataatcgt ttcaaaaccg
     3121 gattcgcgca gggcgagcgc ggcgtgaacg cagcagtagt caaactcgat gccctgaccg
     3181 atgcggttcg ggccgccgcc gagaatcatc acttttttac ggtcggaagg acgggattcg
     3241 cattcttctt cgtaagtgga gtaaagatag gcggtttcgg tggcgaactc ggcggcgcag
     3301 gtgtcaacgc gtttgtaaac cggatgcagc ttcagcgcgt agcggtgttc gcgcacttct
     3361 ttttctttta cgcccaaaag ctgcgcgatg cgtttgtcgg agaagccttt gcgtttcaga
     3421 cggcgtaggg cggcgaaatc caaatcactc aaaatgccgt ctgaaaccga tttttcttct
     3481 ttcaccaagt cttcgatttg cgccaagaac caagggtcga tggcgcagat ttcgtggatt
     3541 tcttccggcg tgaagcccgc gcggaacgcg tctgccacaa acagcatgcg ctcggggccg
     3601 gggtttgcca gttcgcggcg gatttccgct ttgtcggagc ttctcggatt gaagccgcac
     3661 aagcctgttt ccaagccgcg cagggctttt tggaaacttt cctgaatcgt gcgtcccatc
     3721 gccatcactt cgcccaccga tttcatctgc gtggtcaggc ggtcgtctgc ggcagggaat
     3781 ttttcaaacg caaaacgcgg gattttggtt accacatagt cgatggacgg ctcgaatgac
     3841 gcgggggttt tgccgccggt gatgtcgttg cgcaactcgt ccagcgtaaa gccgaccgcc
     3901 agtttcgccg ccaccttcgc aatcgggaag cccgtcgctt tggaagccag cgcggatgaa
     3961 cggctcacgc gcgggttcat ctcaatcaca atcatctcgc cgttttcagg gttcaccgca
     4021 aactgcacgt ttgagccacc cgtgtccacg ccgatttcgc gcaatactgc caacgaagca
     4081 ttacgcatga tttggtattc tttgtctgtc aatgtttgcg ccggcgcaac cgtaatcgag
     4141 tcgcccgtat gcacgcccat cgggtcgaag ttttcaatcg agcaaatgat gatgcagtta
     4201 tcgttcttat cgcgcaccac ctccatctcg tactctttcc agccgaggac ggactgctca
     4261 atcagcagct cgtgcgtggg cgacgcatcg aaaccgcgtt cgcaaatcgc caaaaactcg
     4321 tctttattgt aggcaatgcc gccgcccgaa ccgcccatgg tgaaagaagg acgaatcagg
     4381 gtagggaagc cgacctgttc ttgcgccgcc aaagcttcgt tcatcgtgtg gcagacaaaa
     4441 gatttcgggc aagacaaacc gattttttcc atcgcctctt taaagcggcc gcggtcttct
     4501 gctttgtcga tggcgtcttc cgtcgcgccg attaattcaa cattgtattt cgccagcacg
     4561 ccgttgcgcg ccaaatccag cgcacagttc agcgcggtct gaccgcccat cgttggcaga
     4621 atcgcatcgg gccgctcttt ggcaataatt ttttccaccg tctgccacat aatcggctcg
     4681 atgtaggtaa catccgccat ttcggggtcg gtcataatcg tggcggggtt ggaattcacc
     4741 aaaatgactt tatagccttc ttcacgcaag gctttgcagg cctgtgcgcc cgaatagtca
     4801 aattcgcagg cctgaccgat aacgataggg ccggcgccga tgataaggat ggattttagg
     4861 tcggtacgtt tgggcatggt gggtctttct tttgaagaat ttggattatt gacgattggt
     4921 aacttctcta attatttctg catctgtttt ttcatcgctt ggattatctg aaaagaaaaa
     4981 actgtaatta ctatcggctt taaatttttc gtatagctct gggcgcaaaa tttttgcttt
     5041 ttcactattt tccacgctcc tgcgattagg cttgccagcc ttatctttcc catttctacg
     5101 gacatcatag caataaatag tgttaggaga attaggttct aaacgagtag ctacaatctt
     5161 tttataaatt atgtctaaat cttttttagt ttgtgttgga atgaaatctg caaaaagatt
     5221 actaaaactc tgaaaaattt ctctgatttt cgcagcacca gcctgaacat taattttaaa
     5281 aaaattaata aaatcagtag cacttttaaa atgaacttca taaaaatcag catacgcaaa
     5341 tattgaattt tcactattca aattaaaaaa ctctaatttt gcttgtgcat aatcatctgg
     5401 tctaggttca tcaggtttat acctagtcaa aataacggcg tattgccttt gattataatt
     5461 aaaaggaaat gcttcaatta ttctattttt gtttctcata tcattaagca aatactttaa
     5521 gttattgaaa ttaataggta ttgacatatt taaactcctg cgaatatgtt gcaataaacg
     5581 tagcaatcgt aggttgggtt gaaaacccaa cacaaaccgc ttaattgtct aaaatgttgg
     5641 gtttgctaac ctaacttgta tatcgaaacc cccgtatcgt agcaagccgt agcccgtatg
     5701 tagggtatgt gcggtacgta tgcacgcgtt ctccattttc cctgcaaccc caacccaccg
     5761 cgtgcgtgcc ttgcggcaca ctctctgccg atgagttcaa aggtcgtctg aaaaaacgtt
     5821 cgcaacgttt gttccacctg ccttgcgata aaggctgcct gaaacattcc cgctgtcgat
     5881 gtttcgggca ttctttttca gacgacctgc aacgcctgaa tctgctgcaa cctgccccct
     5941 cccccgtggg ggagggttgg ggagagggca ttccccgaat ttcggcaacc tttcctaaca
     6001 ctctaaccgc caagacacaa gccttgtggc ttgttgccct ctctctaact ctctcccacg
     6061 ggagagagga ctgggcggtt gttggggcta aggtttctgt aaactaatca ggttatgcag
     6121 gctgcttttt gtttttcaga cggcctttat aaatgctcaa ttcagtcggc tttatcctat
     6181 tattttcaat aatccaatca aaatcagcca tctgctatcg tgcagcttgc ttttccaatt
     6241 cttgcaacac gagcaggatt tctgtcagca catcgtttgt ctgctgtaaa atctcgtgat
     6301 tccaaaaccg cagtacggta aagcccaagc tgttgagata tgccgtccgc gcgtggtcgt
     6361 atgcggcttg ttctgtgtgc tgccctccgt ctgcttcgac aatcaacttg ggcgttacgc
     6421 acataaaatc aacaatatag tttcccatcg gctgctggcg gcggaattta tagccgttca
     6481 gacgacctgc ccgcaggtgc tgccacaatt ttgcttccgc cccgctcatt tcttggcgca
     6541 tggctttggc gcgttggcgc aagatggggt tgtcggtggt caagagtttc tcgtgcgggt
     6601 tcattttgtt gtcgtgctgt ttgctgccct ctctctaact ctctcccacg gggagagagg
     6661 acggggcggt tgttggggtt aaaggggctg taaggaaaac aagtcgttca ggccactttt
     6721 tacttttcag acgacctgca atcaacggtt gtaaatccaa gttgcttttc aaaccgcctg
     6781 taaccgcaaa acttgctgca acctaccccc tcccccgtgg gggagggctg gggagagggc
     6841 attctccgaa tcgaggcaat ctttccctaa ttccctaact gcctcaatac aagcctttcg
     6901 gcttgttgcc ctctctctaa ctctctccca cgggagagag gacggtgcgg ctgtagtgat
     6961 taagggtttt gcaaggaaaa caaggaaaac aagttgtaca ggctgttttt tatttttcaa
     7021 gcgacctgaa atcaacgctt gtaaatccaa gtcgcttttc aaactgcctg caaccgcaaa
     7081 atctgctgca acctaccccc tcccccgtgg gggagggctg gggagagggc attttccaag
     7141 ttgcggcaat ctttcccaaa tcccttagtc ttctaaaata caagccttac ggttgttgcc
     7201 aaatgaaact tcttccatac ccaaaacttg cgtgtaaaac gcgatggttc ggtcaatgtc
     7261 ggcaacggtc agtaccagat ggtcgagtgc gctaatcttc atttcccgct ttctttttca
     7321 ggcggcggca gtgccgtctg aaagccatta tgcccgtttt gccgctttca tattgccaat
     7381 gaatttgtca aacaaatagc cgacatcttg cggaccgggg ctggcttcgg ggtggccttg
     7441 gaagcagaac acgggtttgt cggtcagctc gatgccttgc aaagtgttgt caaacaagga
     7501 tttgtgggta atgcgtgcgt tagcgggcag ggtgtcggca tcaacggcaa aaccgtggtt
     7561 ttggctggtg atgacgactt tgccgctgtc caaatcttgc acagggtggt tcgcaccgtg
     7621 gtggctgaag cgcattttca gggttttcgc gccgatagcg aggctgatga gctggtgtcc
     7681 caagcagatg ccgaaaatcg gtttgccgct ttccatcagt ttttgcacgg cttcgatggc
     7741 gtaggtgcaa ggctcggggt cgccggggcc gttggacagg aacacgccgt cgggattgag
     7801 tgccaacacg tcttgtgcgc tcgtttgggc ggggacgacg gtcaggcggc agccgcgcga
     7861 ggcgagcata cgcaggatgt tggttttcac gccgaaatcg taggcgacga cgtggtaagg
     7921 ctgttcgtca ggggtaacga aacccttgcc caattcccat tcgccttccg tccattcgta
     7981 agtttccgtg caggaaactt cttttgccaa gtctttgccg accatactgc cgaacgcggc
     8041 gatgagttct tgcgcttttt caaccgtagc atccgcgccg gtcaagatcg cgccgccttg
     8101 cgcgcctttt tcacgcaaca gcgtggtcag gcggcgggtg tcgatgtcgg cgatggcgac
     8161 ggttttgttg cgtaccaaat agtcgtgcag gctttcggag gcacggaagt tgctgtgcaa
     8221 gagcggcagg tcgcggataa tcaggccggc ggcgtaaacg ctgcggcttt cttcatcttc
     8281 ggcgttggtg ccggtgttgc cgatgtgggg gtaggtgagg gtaacgattt gtttgcagta
     8341 ggacgggtcg gtcaggattt cctgatagcc ggtcatcgaa gtattgaaca cgacttcgcc
     8401 ggaagtcgaa ccttcgtaac cgattgatgt gccgtggaat acgctgccgt cagcgaggac
     8461 gaggagggcg ggggtgctca tgatgggaat cctgttttta ataaaattct tgacaatgcc
     8521 gtctgaaggg acttcggcag ggggcatccg gtcggaaaaa tccggtcaaa aaaaaacacg
     8581 ccgcgcaaga tagacgcgta acgtgctttt cgggcaagtg cctataccct gaaagcagca
     8641 tattttaaga cgaaacatac ccgatttcaa gcgcaaacgc ggcaggcggg ctgatatgtt
     8701 cgcgcgaaaa cggaaatccg gggcataaaa ccgcccgctc cgcgcccact atgcccgtta
     8761 cgcattttcc ccacgtccgc ccggcgaaac tatgggaata cccgaa
//
LOCUS       DAWHSK010000058         8740 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.058, whole genome shotgun sequence.
ACCESSION   DAWHSK010000058 DAWHSK010000000
VERSION     DAWHSK010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8740
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.058"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(83..774)
                     /locus_tag="WIC03_000875"
                     /pseudo
     CDS             complement(83..774)
                     /locus_tag="WIC03_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
     gene            complement(1080..1637)
                     /locus_tag="WIC03_000876"
     CDS             complement(1080..1637)
                     /locus_tag="WIC03_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883400.1"
                     /translation="MMQKHKCLIDFILTVASQIAYADLPLSLEELLTDKGKFKLESSI
                     SYINTERNQSEFANPIYVQTSATKIATVMAMMITPVMAQNLDSQVFDSQNVKAIQLSQ
                     AEMKETQGEFVPIIAAAAFGGALGAWEYHGANLYNHGKLGTAQGAATAAGIGAATGVA
                     AKQGWLLLPEADWLGIWHGDRVFMH"
     gene            complement(1881..2039)
                     /locus_tag="WIC03_000877"
     CDS             complement(1881..2039)
                     /locus_tag="WIC03_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883401.1"
                     /translation="MKTPLPICTLSALAACALSGQGGSRVYGEIKARDLCVTAPILLS
                     AFCLACSR"
     gene            complement(2036..2878)
                     /locus_tag="WIC03_000878"
     CDS             complement(2036..2878)
                     /locus_tag="WIC03_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ3883402.1"
                     /translation="MLVCNPYEVVIHGTTSSGKIFRPSDWAERLCGILSSFTKDHRLS
                     YSKWVRPMLVDNIRCVAVDKKLETDNPQMFRFLMDFAADNDLRVIDCKALLEEREQGG
                     QNNPADEHVMLAQAIEEKHAAEKAQEQTASGASYVLREIGADDTATAFAALSVLRSAL
                     TDINRFTEQINKVQRPQGYRLLGIFEEGKHNAVAVCGFREACTLASGRHIHIDDIVTL
                     PQSRRKGYASRLLEEVRKIGAETGVTKIHLNVHVNHDRADAHRLYFKNGFEICAYHFR
                     CDPK"
     gene            3113..4033
                     /gene="pyrB"
                     /locus_tag="WIC03_000879"
     CDS             3113..4033
                     /gene="pyrB"
                     /locus_tag="WIC03_000879"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HEZ3883403.1"
                     /translation="MPNPLYRQHIISISDLSREQLECLLQTALKLKAHPRGDLLEGKL
                     IGSCFFEPSTRTRLSFETAVQRLGGKVIGFSDGANTSAKKGETLADTARIISGYTDAI
                     IQRHPKDGAARVAAEFSRVPVINAGDGTNQHPSQTLLDLVTIYETQGRLDKLKIAMAG
                     DLKYGRTVHSLCQALKRWGCEFAFVSPPSLAMPDYITEELDEAGCRYRILGSLEEAAE
                     WADILYMTRVQRERFDEQEFAKIQGKFNLEASMLAHAKPNLRVLHPLPRVDEIHPDVD
                     ATPHAYYFEQATNGVYARMAILSLVLNEEV"
     gene            4043..4501
                     /locus_tag="WIC03_000880"
     CDS             4043..4501
                     /locus_tag="WIC03_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HEZ3883404.1"
                     /translation="METPKLSVEAIEKGTVIDHIPAGRGLTILRQFKLLHYGNAVTVG
                     FNLPSKTQGSKDIIKIKGVCLDDKAADRLALFAPEAVVNTIDNFKVVQKRHLNLPDEI
                     AEVFRCPNTNCAGHGEPVKSRFYVKKHNGQTRLKCHYCEKTYSRDSVAEA"
     gene            4566..5231
                     /locus_tag="WIC03_000881"
     CDS             4566..5231
                     /locus_tag="WIC03_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883405.1"
                     /translation="MMMHASVQSRFAPILYVLIFFAGFLTAQIWFNQKAYTEELPPLL
                     SALSAVALVWLAWAFVSARSKAKAEKFYREKMIQNESIHPVLHASLQHLEHKPQMLAL
                     LVKNHGKGMAEQVRFKAEVLPDDEDARTIAAELAKMDMFALGTDAVASGETYGRVFAD
                     IFELSAALEGRAFKGMLKLTAEYKNIFGDACRSETALELGALNQALQEISKTSEKSKR
                     IFY"
     gene            complement(5294..6511)
                     /locus_tag="WIC03_000882"
     CDS             complement(5294..6511)
                     /locus_tag="WIC03_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEZ3883406.1"
                     /translation="MQRRIITLLCAAGMAFSTQTLAANLEVRPNAPERYTVKQGDTLW
                     GISGKYLYSPWQWSRLWDANRDQIHNPDLIYPDQVLVLRHVDGEPRLGLEQTDGIPVV
                     KMSPDKEVSGYGIPAIDVNFYRIFMRHPQIVSRKETAAAPRLLSGPEGRLLYTKGTRV
                     YTKGLKEPGRYLTYRINKNITDPDTGKFLGQEVAFSGIVRSLDYTDSALEQRSKQAGE
                     RLKDNEYHTRTHPLITPLRTPSIQPLVVETAISEIQQGDYLMKMPEDTDRFNMMPHEP
                     SRPVQAKIVSVFEGTRIAGQFQTITIDKGEADGLDKGTVLSLYKRKKTMQVDLSNNFK
                     SRDTVELISTPAEEVGLAMVYRTSEHLSSAIILENISDISVGDTAANPGRDLDNIPDQ
                     GRSRVKFGFNRSE"
     gene            6653..7198
                     /gene="def"
                     /locus_tag="WIC03_000883"
     CDS             6653..7198
                     /gene="def"
                     /locus_tag="WIC03_000883"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEZ3883407.1"
                     /translation="MKNRRRIRRRKEKIMALLNILQYPDERLHTVAKPVEQVDERIRK
                     LIADMFETMYESRGIGLAATQVDVHERVVVMDLTEDRSEPRVFINPVIVEKDGETTYE
                     EGCLSVPGIYDTVTRAERVKVEALNEKGEKFTLEADGLLAICVQHELDHLMGIVFVER
                     LSQLKQGRIKTKLKKRQKHTI"
     gene            7285..8211
                     /locus_tag="WIC03_000884"
     CDS             7285..8211
                     /locus_tag="WIC03_000884"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEZ3883408.1"
                     /translation="MKVIFAGTPDFAAAALKAVAAAGFEIPLVLTQPDRPKGRGMQLT
                     APPVKQAALELGLRVAQPEKLRNNAEALQMLKEVEADVMVVAAYGLILPQEVLDTPKH
                     GCLNIHASLLPRWRGAAPIQRAIEAGDAETGVCIMQMDIGLDTGDVVSEHRYAIQPTD
                     TANEVHDALMEIGAAAVVADLQQLQSKGRLNAVKQPEEGVTYAQKLSKEEARIDWSES
                     AAVIERKIRAFNPVPAAWVEYQGKPMKIRRAEVVAQQGAAGEVLSCSADGLVVACGEN
                     ALKITELQPAGGRRMNIAAFAAGRHIEAGTKL"
     gene            8290..>8740
                     /locus_tag="WIC03_000885"
     CDS             8290..>8740
                     /locus_tag="WIC03_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEZ3883409.1"
                     /translation="MSMALAQKLAADSIAAVAEGRNLQDVLAQIRTAHPDLTAQENGA
                     LQDIAYGCQRYLGSLKHMLAQMLKKPIDNPQLESLLLAALYQLHYTRNAPHAVVNEAV
                     ESIAKIGRGQYRSFANAVLRRFLRERDKLAASCKKDDVAKHNLPLWWV"
BASE COUNT         2120 a         2163 c         2390 g         2067 t
ORIGIN      
        1 ggtttcgtac tatttgtact gtctgcggct tcgtcgcctt gtcctgatta ctatataaaa
       61 taaatgggca aaaatcggtt tattatcgtt tttgccgcat ttggatttgt tctaccgtaa
      121 aacgtgtttg acgaacggga ttcttattaa aaaacatctg atttctaaca aaatcagtat
      181 tttttggcac gatggctaaa atttttcctt ccatttcgcc atcacgtgtt ttccatgcgc
      241 tcaagaattg tgatttgctc attgagacgt gccccagcga tggatcagcc agcaaaacag
      301 tttctccgtt aataccgttc aataccgaaa aatggttgtt tttacggtat tttaaataca
      361 caattacagg aatttttagt tgtaccaact gttcaaatgg caaagcataa ccttgtgctt
      421 caaaacccag ttcgggcatt atgcgttgca tatcatcaaa agaagcacgc atttgggttt
      481 tatccatttt gtccaagatt tccgcttcag aataatgtct gccataaaaa ttattcagta
      541 acgtggcaat cgaagccgcg ccgcaagaaa aatccaaatc ttgttttact atgccggaat
      601 ctcgccgtgc tttccaactt cgtacatgga tgttttggta agaagcgggg ggtcaacaaa
      661 cataggccaa gcaaaaacta tatttggggc gaaaccaatc aaagccgcat aatttatcaa
      721 tttataaaga ttttttatca taatatgtat acgcggaata aacgcatgaa acataaaaaa
      781 ataaaatcat atgcaatttt attgcatgaa taaatatgaa taaaatagat aatgatggga
      841 gtaaatacgc catgtatttt ggaagtttaa atttattaat aataaaataa tttatcgtag
      901 cgcaaataaa tcccaaaatt ggaacgatta gaaaaaaaat taatacaccc atcatcatgc
      961 aaactctata ttaataaata gctaactaat tctattgtgg aaattagtta gctaactaaa
     1021 agttattaat gattatttcc gagaattgac tgcattgttg gcagcattgg ccccaaaacc
     1081 tagtgcatga atacccggtc tccatgccaa attcccagcc aatccgcctc cggcagcagc
     1141 agccagccct gttttgccgc tactcctgtt gccgcaccga ttcctgccgc agtagccgcg
     1201 ccttgcgcag ttcctaattt accatgatta tacaaattag caccatgata ctcccatgca
     1261 cctaatgcac cgccaaaagc agcggctgca ataatgggaa caaattcacc ttgtgtttct
     1321 ttcatttcag cctgtgataa ttgaattgct ttcacatttt ggctgtcaaa aacttggctg
     1381 tctaaatttt gcgccattac aggtgtaatc atcatagcca ttacagttgc aattttcgtt
     1441 gcgctggttt gcacataaat aggattagca aattcgcttt gattgcgttc agtgttgatg
     1501 tagctaatac tgctttctag tttgaattta cccttgtcag tcaataattc ttccaaactt
     1561 aaaggtaagt cagcataagc aatttggctg gcaacagtca aaataaaatc tattagacat
     1621 ttgtgttttt gcatcatttc gtttgatttt ctagtttttg agaatgatac aaagtttttt
     1681 acaaagtaaa gagtcactct gaaaaacatt ttttcattat aaatcaaaat attgatagaa
     1741 taaatagcga gcatcgattc acggtgtact ttagtgcaaa gcgtacaacg cgagcctgaa
     1801 ccaccagccg caacaggaaa agaaagccga tagagtgcaa tgcttgccaa cgtgcaagcg
     1861 agcttgcaag aacgcttggc tcaacgagag caggcaagac agaaagcaga aagcaggata
     1921 ggagcggtaa cgcaaaggtc tcgggctttg atttcgccgt aaaccctgct gccgccttgt
     1981 ccggaaaggg cgcaggcggc gagtgccgac agggtgcaga tggggagggg ggttttcatt
     2041 tggggtcgca acggaagtgg tatgcgcaga tttcaaaacc gtttttgaaa tacaggcggt
     2101 gcgcgtcggc acggtcgtgg ttgacgtgga cgttgaggtg gattttggtt acccctgttt
     2161 ccgcgccgat tttgcggact tcttccaaaa ggcgcgaggc gtagcctttg cggcggcttt
     2221 gcggcagggt aacgatgtca tcgatgtgga tgtggcggcc gctggcgagg gtgcaggctt
     2281 cgcggaagcc gcagacggcg acggcattgt gtttgccttc ttcaaaaata cccagcaggc
     2341 ggtagccttg ggggcgttgg actttgttga tctgttcggt aaagcggttg atgtcggtca
     2401 gggcggaacg caaaacgctc aaggctgcaa aggcggtggc ggtgtcgtcc gcgccgattt
     2461 cgcgcaaaac gtaggatgcg cccgaggcgg tctgttcctg tgctttctcg gcggcgtgtt
     2521 tttcttcgat tgcctgtgcc agcatgacgt gttcgtcggc agggttgttt tgtccgccct
     2581 gttcgcgttc ttcgagcagg gctttgcagt cgatgacgcg caggtcgttg tcggcggcaa
     2641 agtccatcag gaagcggaac atttgggggt tgtcggtttc cagtttttta tcgacggcga
     2701 cgcagcggat gttgtccacc agcatggggc gcacccattt cgaataggaa agcctgtggt
     2761 ctttggtgaa cgaggacaga atgccgcaca ggcgttccgc ccagtcgctg ggacggaaaa
     2821 tcttgccgga actcgttgtg ccgtggatga cgacttcgta ggggttgcag actaacatgg
     2881 cggcttcctg aaaagaaatg tctagcgcga ttatacctta tgcttatgcg ggcgtgtttg
     2941 gatatgccgt ctgaaaagta caggattcgt gcagtaaaac tttgcggcgg caaatgtgcg
     3001 ataatacgcg gcgtattgcc gcttttgcga agctgttccg caaacatacg ggcggcgtgg
     3061 acgtataacc ggatacccgc ctgacgcggg ttttttacgg aagggggcaa aaatgcctaa
     3121 tccgctttac agacagcaca tcatctcgat ttcggatttg tcgcgcgaac agttggaatg
     3181 cctgcttcag acggcattga agctgaaggc gcatccgcgc ggcgatttgt tggaaggcaa
     3241 acttatcggt tcgtgcttct ttgaaccgtc cacgcgcacg aggctgtcgt ttgaaacggc
     3301 ggtgcagcgg ctgggcggca aggtcatcgg cttttccgac ggcgcgaata ccagtgccaa
     3361 aaaaggcgag acgcttgccg ataccgcccg catcatttcc ggctacaccg acgcaatcat
     3421 ccaacgccac cccaaagacg gcgcggcgcg cgtggcagcg gagttttcgc gcgtccccgt
     3481 catcaacgcc ggcgacggca ccaaccagca ccccagccag accttgttgg atctggtaac
     3541 gatttatgaa acgcaggggc gtttggacaa gctcaaaatc gccatggcgg gcgacttgaa
     3601 atacgggcgc accgtgcatt cgctttgtca ggcgttgaaa cgctggggct gcgaatttgc
     3661 ctttgtttcg ccgcccagcc tcgcgatgcc cgactacatt accgaagagt tggacgaagc
     3721 cggctgccga taccgtatcc tcggcagttt ggaagaggcg gcggaatggg cggacatcct
     3781 gtatatgacc cgcgtccagc gcgaacgttt cgatgaacag gaatttgcca aaatccaagg
     3841 caaattcaac ctcgaagcgt ctatgctcgc ccacgccaaa ccgaacctgc gcgtgctgca
     3901 ccctttgccg cgcgtggacg aaatccatcc cgatgtcgat gccacgccgc atgcctatta
     3961 tttcgagcag gcaaccaacg gcgtttatgc gcgtatggcg atattgtcgc tggtgttgaa
     4021 cgaagaagtg tgaggaaccg atatggaaac cccgaaactc agtgtcgaag ccattgaaaa
     4081 aggtacggtt atcgaccata ttcccgctgg cagggggctg accatcctgc gccagttcaa
     4141 acttttgcac tacggcaacg cagtaaccgt gggcttcaac ctgcccagca aaacccaagg
     4201 cagcaaagac atcatcaaaa tcaaaggcgt gtgcttggac gacaaagccg ccgaccgcct
     4261 tgccctgttt gcacccgaag cagtggtcaa caccatcgac aatttcaagg ttgtgcagaa
     4321 acggcatttg aacctgcccg acgaaatcgc cgaagtgttc cgctgtccga acacgaattg
     4381 cgccggccac ggcgagccgg tcaaaagccg gttttatgtt aaaaaacaca acgggcagac
     4441 gcggctgaaa tgccattact gcgaaaaaac ctacagccgg gattcggtgg cggaagcctg
     4501 acggattccc ttaaaccgag cgggcggcat ttcgtctgcc gcttgttttg ccaatctgaa
     4561 atggaatgat gatgcacgct tctgtccaaa gccgtttcgc accgatactt tatgttttga
     4621 ttttctttgc cggttttttg accgcgcaaa tctggttcaa tcagaaagcc tatactgaag
     4681 agctgcctcc gcttctgtcc gcattgtccg ctgtcgcgct ggtgtggctg gcgtgggcgt
     4741 tcgtgtcggc gcgttcaaag gctaaggcgg aaaagttcta ccgcgaaaaa atgatacaga
     4801 acgaaagcat acaccccgtc ctgcacgctt ctttgcaaca cttggaacac aagccgcaaa
     4861 tgctcgccct gctggtcaaa aaccacggca aagggatggc ggaacaggtc aggttcaagg
     4921 cggaagtgct gcccgacgac gaagacgcgc gcacgattgc cgccgagttg gcaaaaatgg
     4981 atatgtttgc attggggacg gacgcggtcg cctcgggcga aacctatgga cgcgtgttcg
     5041 ccgatatttt cgagttgtcg gcggctttgg aagggcgcgc gttcaaagga atgttgaaac
     5101 tgacggcgga atataaaaac atcttcggcg atgcctgccg ttcggaaacg gcgttggagt
     5161 tgggcgcgct caatcaggcg ttgcaggaga tttcaaaaac atcggaaaag tccaaacgga
     5221 tattttattg aagatggaaa aatgccgtct gaaacggaag gtgtttcaga cggcattttt
     5281 gtcggatgat taattattcg gagcggttga agccaaactt cacgcggctg cggccctgat
     5341 ccggtatatt gtccaaatcg cgtcccggat tggcggcggt gtcgcctacg gaaatatcgg
     5401 agatgttttc caaaatgatg gcggacgaca ggtgttcgga ggtgcggtaa accattgcca
     5461 agcccacttc ttcggcagga gtggaaatca gctcgacggt atccctgctt ttgaaattgt
     5521 tggagaggtc gacctgcatc gttttcttgc gtttgtagag gctcaaaacc gtgcctttgt
     5581 ccaaaccgtc cgcctcgcct ttgtcgatgg tgatggtttg aaactggccg gcaatccttg
     5641 tgccttcaaa cacggaaacg attttagcct gaaccgggcg ggacggttcg tgcggcatca
     5701 tgttgaagcg gtcggtgtct tccggcattt tcatcaggta gtcgccctgc tgtatttcgg
     5761 aaatggcggt ttcgaccacc agcggctgta tcgaaggggt gcgcagcggg gtaatcaaag
     5821 gatgggtgcg ggtatggtat tcgttgtctt tcagccgttc tccagcctgt ttcgagcgtt
     5881 gttcgagggc ggagtcggta tagtcgaggg agcgcacgat gccgctgaat gcgacttcct
     5941 gcccgaggaa tttacccgta tccggatcgg tgatgttttt attgattcgg taggtcaggt
     6001 agcggcccgg ctctttcagg cctttggtgt aaaccctggt gcctttggtg tacagcagcc
     6061 tgccttccgg gcccgagagc aggcgcggcg cggcagcggt ttctttgcgg gaaacgattt
     6121 gcggatgccg cataaagatg cggtagaagt tgacatcgat ggcgggaata ccgtatccgg
     6181 acacttcctt atccggactc attttgacga cggggatgcc gtctgtctgt tccaagccga
     6241 ggcgcggttc gccgtcaacg tggcgcaaca ccaatacctg gtccggataa atcaggtcgg
     6301 gattgtggat ttgatcccgg ttcgcgtccc acaggcggct ccattgccac gggctgtaca
     6361 ggtatttgcc cgaaatgccc cacagggtgt cgccctgttt gaccgtgtag cgttccggcg
     6421 cgttcgggcg cacctccaaa tttgccgcca aagtttgtgt tgagaatgcc atacctgccg
     6481 cgcagagcag ggttataata cgacgttgca taaccgttcc ccttatctga taaatttcgg
     6541 tttgtcttgc ttgattgggt tggaaaaagc ggcggcagcc cctcgggatg tgccgcgtga
     6601 taaaaaatgt tccgcatttt aacatcgaat tatccgcacc atcacggtaa ttatgaaaaa
     6661 caggcggcgt atccgccgaa ggaaagagaa aattatggct ttattgaata tcttgcaata
     6721 tcccgacgag cgtctgcaca cggtggcaaa gcctgtcgaa caagtcgacg agcgcatccg
     6781 gaagctgatt gccgatatgt ttgaaacgat gtacgaatcg cgcggcatcg ggctggcggc
     6841 gacgcaggtc gatgtgcacg agcgcgtggt cgtgatggat ttgaccgaag accgcagcga
     6901 accgcgcgtg ttcatcaacc ccgtcatcgt tgaaaaagac ggcgaaacca cttacgaaga
     6961 gggctgcctg tccgtgccgg gcatttacga caccgtaacc cgcgccgaac gcgtcaaggt
     7021 cgaggctttg aacgaaaaag gcgaaaagtt cacgctggag gcggacggct tgctggcgat
     7081 ttgcgtgcag cacgagttgg accacctgat gggcatcgtg tttgtcgaac gcctgtcgca
     7141 gctcaagcag ggacgcatca aaaccaagct gaaaaaacgt cagaaacata cgatttgacc
     7201 cttttgccgt gccgtctgaa cgctgcaaag ttttcagacg gcacggtctt gtccgacaac
     7261 cttacgcata cgcaggaata tgctatgaaa gtcatctttg ccggcacgcc cgattttgcc
     7321 gccgccgcct taaaagccgt tgccgccgcc ggttttgaaa tcccgctggt gctgacccag
     7381 cccgaccgtc cgaaagggcg cggtatgcaa ctgaccgccc cgcccgtcaa acaagccgcg
     7441 ctggaactcg gtttgcgcgt ggcacagccc gaaaaactgc gtaacaacgc cgaagccctg
     7501 caaatgctca aagaggtcga ggcagacgta atggtggttg ccgcctacgg tttgattctg
     7561 ccgcaggaag tgttggatac gccgaaacac ggctgcctca acatccacgc ttcgctgtta
     7621 ccccgttggc gtggcgcggc gccgattcaa cgcgcgattg aagccggcga tgccgagaca
     7681 ggcgtgtgta ttatgcagat ggacatcggt ttggacaccg gcgatgtggt cagcgaacac
     7741 cgctacgcca tccaaccgac cgataccgcc aacgaagtcc acgacgcgct gatggaaatc
     7801 ggtgcggcgg cggttgttgc cgatttgcaa cagcttcaaa gcaaaggccg tctgaacgcg
     7861 gtcaaacagc ccgaagaagg tgttacttac gcccaaaaac taagcaaaga agaggcgcgt
     7921 atcgattgga gcgaaagcgc ggcggttatc gaacgcaaaa tccgcgcctt caaccccgtg
     7981 cctgccgcgt gggttgagta tcagggcaag ccgatgaaaa tccggcgggc ggaagtggtg
     8041 gcgcaacaag gcgcggcagg cgaagtgttg tcctgttcgg cggacggttt ggtcgttgcc
     8101 tgcggcgaaa acgcgctgaa gattaccgaa ttgcagcctg ccggcggcag gcgtatgaat
     8161 atcgcggcgt ttgcagcagg acggcatatc gaagcaggga cgaagctgta aatcccttca
     8221 gacggcattc cgatccgcaa atgggaatgc cgtctgaagt catcagttga agaaagcgaa
     8281 tcacataata tgagtatggc acttgcccaa aaacttgccg ccgacagcat tgcggcggtt
     8341 gccgaaggac gcaaccttca ggacgtgttg gcgcaaatcc gcaccgcgca tcccgacctt
     8401 acggcgcagg aaaacggcgc gttgcaggac atcgcctacg gttgccagcg ttatttgggc
     8461 agtttgaaac atatgctcgc gcagatgctg aaaaaaccga ttgacaatcc gcagctcgaa
     8521 agcctgcttt tggcggcgtt gtaccaactg cattacacgc gcaacgcgcc gcacgccgtg
     8581 gtcaatgagg cggtggaaag catagccaaa atcgggcgcg ggcagtaccg ttcgtttgcc
     8641 aacgcggttt tgcgccgctt tttgcgcgaa cgcgacaagc tcgcggcttc ctgtaaaaaa
     8701 gacgatgtgg cgaaacacaa cctgccgctg tggtgggtgg
//
LOCUS       DAWHSK010000059         8691 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.059, whole genome shotgun sequence.
ACCESSION   DAWHSK010000059 DAWHSK010000000
VERSION     DAWHSK010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8691
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.059"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            191..1726
                     /locus_tag="WIC03_000886"
     CDS             191..1726
                     /locus_tag="WIC03_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEZ3883410.1"
                     /translation="MSDSKTKERATFGTRRAFMIAAIGSAVGLGNIWRFPYIAFENGG
                     GAFILPYLVALLTAGIPLLLLDYAIGHRYRGSAPLAFRRLGRWFEPVGWWNVMTNIVI
                     CIYYAVIIGWAASYTYYSVNAAWGADPQGFFFKDFLQMAGPEALGLDFVGKVAGPLAG
                     VWVFTAAIMALGVQKGVARASSFFMPLLLVMFLIMVGISLTLPGAAKGLDALFTPDWS
                     KLADSKVWVAAYGQIFFSLSICFGIMVTYSSYLKKKTDLGGTGLVVGFANSSFELLAG
                     IGVFAALGFMAQAGGKAVNEVASGGIGLAFIAFPTIINQAPMGWLIGILFFGSLVFAG
                     VTSMISILEVIVAAIQDKLNIGRVNATLLVCIPMGIVSTLLFGTATGLPVLDVMDKFV
                     NTYGIVAAGFVYVAAIIISGRLPELRKHLNALSSIRIGGLWTVCVVVTVVMLGYMLFK
                     DTSGLMEKNYEGYPDGFLSIFGWGMSAALVVFGLLLSLLPWKHGQDFNVKDEHEHEQG
                     EEK"
     gene            1723..1815
                     /locus_tag="WIC03_000887"
     CDS             1723..1815
                     /locus_tag="WIC03_000887"
                     /inference="COORDINATES: protein motif:HMM:NF033493.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine/alanine import family NSS transporter
                     small subunit"
                     /protein_id="HEZ3883411.1"
                     /translation="MSTSAIVMMIVSIVIIWGGLLLSLLRLPNE"
     gene            complement(1875..3095)
                     /gene="lysA"
                     /locus_tag="WIC03_000888"
     CDS             complement(1875..3095)
                     /gene="lysA"
                     /locus_tag="WIC03_000888"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEZ3883412.1"
                     /translation="MTLFCEQVPYPRLAEEFGTPLYVYSKSALTEAFENYQTAFATLN
                     PLVCYAVKANGNLSIIKHFASLGSGFDIVSDGELARVLAAGGDAAKTIFSGVGKSEAE
                     IEFALNAGVKCFNMESIPEIDRIQKVAARLGKVAPVSLRVNPDVDAKTHPYISTGLKA
                     NKFGIAYADALEAYRHASRQSHLKIVGIDCHIGSQLTDLSPLVEACERILVLVDQLAD
                     EGIVLEHLDLGGGVGIVYQDENVPDLGAYAQAVQKLIGTRRLKLILEPGRSLVGNAGS
                     LLTRVEFVKYGEEKNFVMVDAAMNDLMRPALYDAYHHIEAVETKDIEPLTANIVGPIC
                     ETGDFLGKDRTIACEEGDLLLIRSAGAYGASMASNYNARNRAAEVLVDGNEYRLIRRR
                     ETLEQQMANELACL"
     gene            complement(3106..3276)
                     /locus_tag="WIC03_000889"
     CDS             complement(3106..3276)
                     /locus_tag="WIC03_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEZ3883413.1"
                     /translation="MKYGVFFAAATALLLSACGYKGDLYLPKEGDTARFGAIQTGLQL
                     QNKPQSAPQTRK"
     gene            3347..3670
                     /gene="cyaY"
                     /locus_tag="WIC03_000890"
     CDS             3347..3670
                     /gene="cyaY"
                     /locus_tag="WIC03_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HEZ3883414.1"
                     /translation="MMTESEFIRASEALFEHIEDQIDENGWDFDCRFAGNVLTIEAEN
                     GTQIIVNRHTPNQELWIAAKSGGYHFAEQNGKWLATRDGRDFYDVLNEALSAASGEAV
                     EIEEL"
     gene            3697..4713
                     /locus_tag="WIC03_000891"
     CDS             3697..4713
                     /locus_tag="WIC03_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HEZ3883415.1"
                     /translation="MNTRPFYFGLIFIAIIAILANYLGNTDFSHHYHISALIIAILLG
                     MAIGNTIYPQFSTQVEKGVLFAKGALLRTGIVLYGFRLTFGDIADVGLNAVVTDAIML
                     ISTFFFTALLGIRYLKMDKQLVYLTGAGCSICGAAAVMAAESVTKAESHKVSVAIAIV
                     VIFGTLAIFTYPLFYTWSQDLINAHQFGIYVGSSVHEVAQVYAIGENINPIVANTAVI
                     SKMIRVMMLAPFLLMLSWLLTRSDGVSENTSHKITIPWFAVLFIGVAIFNSFDLLPKE
                     LVKLFVEIDSFLLISSMAALGLTTQASAIKKAGLKPFVLGILTYLWLVVGGFLVNYGI
                     SKLI"
     gene            4739..5158
                     /locus_tag="WIC03_000892"
     CDS             4739..5158
                     /locus_tag="WIC03_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="HEZ3883416.1"
                     /translation="MAQLPLYRTAEIENFTVGTPEVLESFSKHIPYGVVFEDDGDTGY
                     FYAASQDGILDALHIYNVEDVSDKHIPNHVLILWDDACTIAALCINDYIHAVYDFVEQ
                     AGYCRNGFPEAQGEWPKVTDRVLDDELQDKILSRKPT"
     gene            5180..5686
                     /gene="luxS"
                     /locus_tag="WIC03_000893"
     CDS             5180..5686
                     /gene="luxS"
                     /locus_tag="WIC03_000893"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEZ3883417.1"
                     /translation="MPLLDSFKVDHTRMHAPAVRVAKTMTTPKGDTITVFDLRFCVPN
                     KEILPEKGIHTLEHLFAGFMRDHLNGNGVEIIDISPMGCRTGFYMSLIGTPSEQQVAD
                     AWLASMQDVGNVKDQSKIPELNEYQCGTYQMHSLAEAQQIAQNVLARKVAVNKNEELT
                     LDEGLLNA"
     gene            5831..8620
                     /gene="polA"
                     /locus_tag="WIC03_000894"
     CDS             5831..8620
                     /gene="polA"
                     /locus_tag="WIC03_000894"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEZ3883418.1"
                     /translation="MSNRPTLLLVDGSSYLYRAYHAMAQLTAPDGAPTGAMYGVLNML
                     RRLRADYVHDYCAVVFDAKGKNFRHEMFPDYKATRPPMPDDLRPQAEALPDLVRLIGW
                     PVLVIPQVEADDVIGTLAAMAGEAGWNVVVSTGDKDMAQLVNERVTLVNTMSGETLDI
                     EGVKAKFGVRPDQIRDYLALMGDKVDNVPGVEKCGPKTAVKWLEAYGSLAGVMEHAAE
                     IKGKVGENLRAALQRLPLSYDLVTIKTDVDLHTELSDGIESLRRTAPKWAQLVVDFKR
                     WGFRTWLKEAESRMHEAADGDLFGSDSIGEQAALNAEVPSEKQPELAPTPEKLDYQAV
                     TTEAQLAALLDKLSQADTIGIDTETTSLDAMNASLVGISIAFQAGEAIYIPVGHSLTA
                     APEQIDLQDVLGRLKPHLENPALKKIGQNLKYDQHVFANYGIALNGIAGDAMLASYII
                     ESHLGHGLDELSERWLGLETITYESLCGKGAKQISFADVAIGQATEYAAQDADFALRL
                     EAHLRAQMDAKQLEMYEKMELPVAQVLFEMERNGVQIDRAELSRQSAELGAELMKLEQ
                     EAYAVAGQPFNLNSPKQLQEILFDKMGIPTKGLKKTAKGGISTNEAVLEQLAPDYPLP
                     KIILQNRSLAKLKSTYTDKLPEMISPKDGRVHTTYAQAVAITGRLASNNPNLQNIPIR
                     TAEGRRVRRAFTAPQGSVIVSADYSQIELRIMAHLSGDKTLITAFQNGEDVHRRTAAE
                     VFGIAPENVSSEQRRYAKTINFGLIYGMGQYGLAKSLGIDNISAKTFIDRYFARYPGV
                     AEYMQRTKEQAAAQGFVETLFGRRLYLPDIRNKNANARAGAERAAINAPMQGTASDLI
                     KRAMIDVRNCLSDGIGSKLVMQVHDELVLEVVETELDFVKEKLPQIMAKVDGGLLDVP
                     LVAEVGVGENWEEAH"
BASE COUNT         2052 a         2281 c         2267 g         2091 t
ORIGIN      
        1 aaatttttcg ttttcaagcc ttcaccgctt gccatcagcg ttaaattttt ttacgataag
       61 cacatagatt gtaaacaatc ggccacaagc cggtttgttt tttcagaaga cattatccct
      121 gtcagacgct atttctatat atttcgccta taacggcttg tttttaataa ataattcaag
      181 aggtatcaac gtgtctgatt ccaaaacgaa agaacgcgcc acattcggca cgcgccgcgc
      241 gtttatgatt gccgccatcg gatccgccgt cggcttgggc aatatttggc gtttccccta
      301 tattgctttt gaaaacggcg gcggcgcgtt catcctgccc tatctggtcg cgcttctgac
      361 ggcgggcatt ccgctgctgc tgctcgatta tgccatcggc caccgttacc gtggttctgc
      421 gcccttggct ttccgccgcc tcggacgctg gtttgagccg gtcggctggt ggaacgtgat
      481 gaccaatatc gtcatctgca tctattacgc ggtaattatc ggttgggcgg caagctatac
      541 ctattattcg gtcaacgccg cctggggtgc ggatccgcag ggttttttct ttaaagactt
      601 cctgcaaatg gcgggcccgg aagccttggg tttggatttt gtcggcaaag tggccggtcc
      661 tttggcgggc gtgtgggttt ttaccgccgc cattatggct ttgggcgtgc aaaagggcgt
      721 ggcgcgcgcc tcgtcgttct ttatgccgct gcttttggtg atgtttttga ttatggtcgg
      781 catttcgctg acgttgccgg gcgcggcaaa gggcttggac gcattgttta cgcccgactg
      841 gtcgaaactc gccgattcca aggtctgggt ggcggcatac gggcagattt tcttttcgct
      901 ttccatctgc ttcggcatta tggttaccta ttcttcttat ttgaagaaaa aaaccgactt
      961 gggcggaacg gggctggtgg tcggttttgc caacagcagc tttgaactgc tcgcgggcat
     1021 cggcgtgttt gccgcattgg gctttatggc gcaggcgggc ggtaaggcgg tcaacgaggt
     1081 tgcctcaggc ggcatcggtt tggcgtttat cgcctttccg accattatca accaggcacc
     1141 gatgggctgg ctgatcggca tattgttttt cggttcgctg gtattcgccg gcgttacgtc
     1201 gatgatttcc atccttgaag tgattgtggc ggcgattcag gacaagctga acatcgggcg
     1261 cgtcaacgcc acgctgctgg tctgcattcc gatgggcatt gtttccacgc tgctgttcgg
     1321 tacggcgacg gggctgccgg ttttggacgt gatggacaaa ttcgtcaaca cctacggcat
     1381 tgttgccgcc ggctttgttt atgttgccgc catcatcatt agcggcaggc tgccggaatt
     1441 acgcaagcac ctgaacgctt tgtcctccat ccgcatcggc ggcttgtgga cggtctgcgt
     1501 cgtggttacc gtcgtgatgc tcggctatat gctgtttaaa gataccagcg gcctgatgga
     1561 gaaaaattac gaaggttatc cggatggttt cctcagtatt ttcggctggg ggatgtcggc
     1621 ggcgttggtc gtgttcgggc tgctgctgtc gttgctgcct tggaaacacg gtcaggattt
     1681 caacgtcaaa gacgaacacg aacatgaaca aggagaagaa aaatgagtac ttccgccatt
     1741 gtgatgatga ttgtctcaat cgtgataatc tggggagggc tgctgctttc cctgttaagg
     1801 ctgccgaacg agtaagcctt tagagcgtta aaaatgccgt ctgaactgtt tcagacggca
     1861 ttttgatgtt cggcttacag gcaggcgagt tcgtttgcca tttgctgttc caaggtttcg
     1921 cgccggcgga tgagtcggta ttcgttgccg tccaccaaca cctctgccgc acggttgcgc
     1981 gcgttgtaat tgctcgccat actggccccg tatgcgcccg cgctgcggat aagcagcaaa
     2041 tccccttctt cgcaggcgat ggtgcggtct ttgccgagga agtcgccggt ttcgcaaatc
     2101 ggaccgacga tgttggcggt cagaggctca atgtctttgg tttcgaccgc ctcgatgtga
     2161 tgataggcat cataaagcgc cgggcgcatc aaatcgttca tcgccgcatc gaccatcaca
     2221 aagtttttct cttcgccgta tttgacgaat tcgacgcgtg tcagcagcga acctgcgttg
     2281 ccgaccaagc tgcggccggg ctcaagaatg agtttcagac ggcgtgtgcc gatcagtttt
     2341 tgaacggctt gggcatacgc gcccaaatca ggcacatttt catcttggta aacaatgccg
     2401 acaccgccgc ccaagtccaa gtgttccaac acaatgcctt cgtctgccag ttgatcgacc
     2461 aagaccaaga tacgttcaca ggcttcgaca agcgggctga ggtcggtcag ttgcgaaccg
     2521 atatggcagt cgatgccgac aattttcaaa tggctttgtc gggaagcatg acgataggcc
     2581 tcaagcgcat cggcataggc aataccgaat ttattggctt tcaggccggt ggagatataa
     2641 ggatgggttt tagcgtcgac atcggggttg acgcgcaggg aaaccggggc gactttaccc
     2701 aaacgcgcgg caactttctg aatgcggtcg atttcgggaa tgctctccat attgaagcat
     2761 ttcacgcctg cattcagcgc aaactcaatt tctgcttcac tcttgcccac gccggaaaaa
     2821 atcgttttcg ccgcatcgcc gcctgccgct aaaacacgcg ccaactcgcc gtcggacaca
     2881 atgtcaaaac cgctccccaa agaagcaaag tgcttgatga tgctcaggtt gccattggct
     2941 ttcactgcgt agcagacaag cggatttaaa gtggcaaacg cggtttggta gttttcaaat
     3001 gcctcggtca gcgcagattt gctgtacaca taaagcggcg taccaaactc ttcggcaagg
     3061 cgggggtagg ggacttgttc gcaaaacagg gtcatatctt cgttttcatt ttcgggtttg
     3121 tggggcggat tgcggtttgt tttgaagctg caaaccggtt tggattgcgc cgaaacgcgc
     3181 cgtatcgcct tctttgggca ggtagaggtc gcctttgtaa ccgcaggcgg aaagcaggag
     3241 ggcggttgcc gccgcaaaaa atacgccgta tttcatcggt aaacttcctt cataagcgcg
     3301 aatgtggcaa gattcggtat cttaaacaaa aaccacacaa aaagccatga tgaccgaaag
     3361 cgagtttatc cgcgcgagcg aagcattatt tgaacacatc gaagaccaaa tcgacgaaaa
     3421 cggctgggat ttcgactgcc ggtttgccgg aaacgtcctg accatcgaag ccgaaaacgg
     3481 cacgcaaatc atcgtcaacc gccacacgcc caaccaagaa ttatggattg ccgccaaaag
     3541 cggtggctac catttcgccg aacaaaacgg caaatggctg gcaacgcgcg acggacggga
     3601 tttttacgac gttttaaacg aagccctgag cgcggcttcg ggcgaagcgg tagagattga
     3661 ggaattgtga tttgggtgtt atcacggaaa gaaaaaatga acacacgtcc cttttatttc
     3721 ggacttatat ttatcgcgat tattgctata cttgctaact atttaggaaa cactgatttt
     3781 tcccatcatt atcatatcag tgctttaatt attgctatct tgctgggaat ggcaatcggc
     3841 aataccattt atccgcaatt ttcaacacaa gtggaaaaag gtgttttgtt tgccaaaggc
     3901 gcgcttcttc gcactggcat tgtgttgtat ggttttcgcc tcacttttgg cgatattgcc
     3961 gatgtaggat taaatgcggt tgtcactgat gcaatcatgc taatttcaac cttctttttt
     4021 accgcacttt taggcattcg ttatctaaaa atggataaac aattggttta tctcactggg
     4081 gcaggttgca gcatttgcgg tgcggcagcg gttatggcgg cagagtctgt taccaaagca
     4141 gaatcccata aagtttcagt ggcgattgcc atagtggtca ttttcgggac gcttgctatt
     4201 tttacttacc ccttgttcta cacgtggtca caagatttaa ttaacgccca tcaattcggt
     4261 atttatgttg gttctagtgt acacgaagtg gctcaagtgt atgcgattgg ggaaaatatt
     4321 aatcctatcg tggcgaatac tgccgtcatt tccaaaatga tccgagtgat gatgctcgcc
     4381 ccctttttat taatgctttc ttggttatta acacgtagtg atggggtatc agaaaatacc
     4441 tcacacaaaa ttacaattcc ttggtttgct gtacttttta ttggtgttgc catttttaat
     4501 tcttttgatt tattaccaaa agaactcgtg aaattattcg ttgaaatcga ttctttctta
     4561 ttaatttcat caatggctgc gcttggctta acgacgcaag caagcgcaat caaaaaggca
     4621 ggattaaaac cgtttgtttt aggaatacta acttatttat ggctagtggt tggtggattt
     4681 ttagtgaact atggaatatc aaaattaata taaaattcac taaagagagc gttacccaat
     4741 ggcacaatta cccctgtatc ggactgccga aatcgaaaat tttactgtcg gcacgcccga
     4801 agttttagaa tcattttcca aacatatccc ttatggtgtt gtctttgaag acgacggcga
     4861 cacaggctac ttctatgccg cttcgcaaga cgggatttta gatgccttgc acatttacaa
     4921 tgtcgaagat gtatccgata aacatatccc caatcatgtc ttgattttat gggatgatgc
     4981 ctgcaccata gccgcattgt gtatcaacga ctacattcat gccgtctatg attttgtcga
     5041 acaggcagga tattgccgca acggcttccc tgaagcacaa ggcgaatggc cgaaagtcac
     5101 agaccgcgta ttggacgatg aattgcagga caaaatccta tcccgaaaac ctacataacc
     5161 tactcaaaag gataacccaa tgcccctact agacagtttc aaagtcgatc acacccgtat
     5221 gcacgccccc gccgtacgcg tggcgaaaac catgaccacg cccaaaggcg acaccattac
     5281 cgtattcgac ctgcgtttct gcgtgcccaa caaagaaatc ctgcccgaaa aaggcatcca
     5341 cacgctggag catttgttcg ccggttttat gcgcgaccac ttgaacggca acggcgtgga
     5401 aattatcgac atttccccga tgggctgccg caccggtttc tacatgagcc ttatcggcac
     5461 gccttccgaa cagcaggtcg ccgatgcatg gctcgcctcg atgcaggatg tgggcaatgt
     5521 caaagaccaa agcaaaatcc ccgagttgaa cgaataccaa tgcggcactt atcaaatgca
     5581 ctcgctcgcc gaagcgcagc aaatcgcgca aaacgtgttg gcgcgcaaag tggcggtgaa
     5641 caaaaacgaa gagctgacgc tggatgaagg gctgctgaac gcctaatccg ccaaaaatgc
     5701 cgtctgaaca agggtttcag acggcatttg ccttttccgt tataatccgg ggttgtgcgg
     5761 ggtcgggttt taagccggca tcgtccttcc ctattttttc tgtcccttat cggttttaag
     5821 cgggtttttt atgtccaaca gacctacact cctcctcgtt gacggctcgt cctacctcta
     5881 ccgcgcgtat cacgcgatgg cgcagttgac cgcgcccgat ggtgcgccga cgggcgcgat
     5941 gtatggcgtg ttgaacatgc tgcgccggtt gcgggcggat tatgtgcatg attattgcgc
     6001 ggtggtgttt gatgcgaagg gcaagaattt ccgccacgag atgttccccg actacaaggc
     6061 gacgcgcccg ccaatgccgg acgatttgcg tcctcaagcg gaagccttgc cggatttggt
     6121 gcggttgata ggctggccgg tgctggtgat tccgcaagtc gaagcggatg acgtgattgg
     6181 cacgctggcg gcaatggcag gcgaagcagg ttggaatgtg gtggtgtcca ccggcgataa
     6241 ggacatggcg cagttggtga acgaacgcgt aacgctggtg aacacgatga gcggcgaaac
     6301 gctggacatt gaaggtgtga aggcgaaatt cggcgtgcgc cccgaccaaa tccgcgatta
     6361 tctcgcgctg atgggcgaca aggtggacaa cgtgccgggc gtggaaaagt gcggcccgaa
     6421 aacggcagtg aaatggctgg aagcctacgg ctcgctggcg ggcgtgatgg aacacgcggc
     6481 ggaaatcaag ggcaaagtgg gcgagaatct gcgtgccgcc ctgcagcgcc tgccgctgtc
     6541 gtatgatttg gtgacgatta aaaccgatgt ggacttgcac accgaacttt cagacggcat
     6601 cgaaagcctg cgccgtaccg cgccgaaatg ggcgcaactg gttgtcgatt tcaaacgctg
     6661 gggcttccgc acctggctga aagaagcgga aagtcgtatg cacgaagcgg cggacggtga
     6721 tttgttcggc agcgacagca tcggcgagca ggcggctttg aatgcggagg tgccgtctga
     6781 aaaacaaccc gaacttgccc cgacccccga aaaactggat tatcaagccg ttaccaccga
     6841 agcgcagctt gccgccttgt tggacaaact gtcgcaggcg gacacaatcg gcatcgatac
     6901 ggaaaccacg tcattagacg cgatgaacgc ctcgttagtc ggcatcagca tcgcgttcca
     6961 agcaggcgaa gcgatttaca tccccgtagg gcacagcctg accgctgcac ccgaacagat
     7021 tgatttgcaa gacgtattag gccgtctgaa accgcatttg gaaaaccccg ccctgaaaaa
     7081 aatcgggcaa aacctcaaat acgaccaaca cgttttcgcc aactacggca ttgccttaaa
     7141 cggcattgcc ggcgacgcga tgcttgcttc ctacatcatc gaaagccacc tcggacacgg
     7201 cttggacgaa ttgtccgaac gctggctggg cttggaaacc attacctacg aatcgctgtg
     7261 cggcaaaggc gcgaagcaaa tcagttttgc cgatgtcgcc atcggacagg cgaccgaata
     7321 cgccgcccaa gacgccgatt tcgccctgcg cctcgaagcg catctgcgtg cgcaaatgga
     7381 cgccaaacag cttgaaatgt atgaaaaaat ggagctgccc gtcgcgcagg tattgtttga
     7441 aatggaacgc aacggcgtgc aaattgatcg cgccgaactc tcccgccaaa gcgcggaact
     7501 cggcgcagag ctgatgaagc tcgaacaaga agcctatgcc gtcgcaggcc agccgtttaa
     7561 cctcaactcg cccaaacagc tccaagaaat cctgttcgac aaaatgggca ttcccaccaa
     7621 aggcctgaaa aaaaccgcca aaggcggcat ttccaccaac gaagccgtgc tcgaacagct
     7681 cgcacccgac taccccctgc ccaaaatcat cctgcaaaac cgcagcctgg cgaagctcaa
     7741 atccacctac accgacaaac tgcccgaaat gatttccccc aaagacggcc gcgtgcatac
     7801 cacctacgcc caagccgtcg ccattaccgg ccgtctcgcc agcaacaacc ccaacctgca
     7861 aaacatcccc atccgcaccg ccgaaggccg ccgcgtgcgc cgcgccttta ccgcaccgca
     7921 aggcagcgtc atcgtttccg ccgactattc ccaaatcgag ctgcgcatca tggcgcacct
     7981 ctccggcgac aaaaccctga ttaccgcgtt ccaaaacggc gaagacgtac accgccgcac
     8041 cgccgccgaa gtgttcggca ttgcccccga aaacgtctcg tccgaacaac gccgctacgc
     8101 caaaaccatc aacttcggtc tcatttacgg catgggccaa tacggcctcg ccaaatcatt
     8161 gggtatcgac aacatctccg ccaaaacctt catcgaccgc tacttcgccc gctatcccgg
     8221 cgtcgccgaa tacatgcagc gcaccaaaga acaagcagca gcgcaaggct tcgtcgaaac
     8281 tctgttcggc cgccgcctct acctgcccga catccgcaac aaaaacgcca acgcccgcgc
     8341 cggagccgaa cgcgccgcca tcaacgcccc catgcaaggc accgcctccg acctcatcaa
     8401 acgcgccatg atagacgtgc gaaactgtct ttcagacggc atcggcagca aactggtcat
     8461 gcaggtgcat gacgaactgg tgctggaagt cgttgaaacc gaactggatt ttgtcaaaga
     8521 aaaactgccg cagattatgg cgaaagtgga cggcggatta ttggatgtac cgctggtggc
     8581 tgaggttggc gtaggggaga attgggaaga ggcacattga ttgaaaggtg ttatatgcta
     8641 tctttattta aataaaattt aatttttggt atattttttc taaatgttcc t
//
LOCUS       DAWHSK010000060         8628 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.060, whole genome shotgun sequence.
ACCESSION   DAWHSK010000060 DAWHSK010000000
VERSION     DAWHSK010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8628
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.060"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..1337
                     /gene="uvrA"
                     /locus_tag="WIC03_000895"
     CDS             <1..1337
                     /gene="uvrA"
                     /locus_tag="WIC03_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEZ3883419.1"
                     /translation="RLASQIGSGLTGVMYVLDEPSIGLHQRDNDRLLATLKRLRDLGN
                     SVIVVEHDEDAIREADFVVDMGPGAGEHGGNVLIADTPENVAKCENSVTGQYLSGKKS
                     IAVPSERTPVNPDRMLVLKGARGNNLKNVTLELPLGLITCITGVSGSGKSTLINDTLA
                     KITARELNRAQEEPAPYDDIRGLEHLDKVINVDQSPIGRTPRSNPATYTGLFTPIREL
                     FAGVPLSRERGYNVGRFSFNVKGGRCEACQGDGVIKVEMHFLPDVYVPCEVCHGKRYN
                     RETLEIQYKGKNISQVLDMTVEEAREFFDAVPTVSRKLQTLMDVGLGYIRLGQSATTL
                     SGGEAQRVKLALELSKRDTGRTLYILDEPTTGLHFADIALLLEVIDRLKGKGNSIVII
                     EHNLDVIKTADWIVDLGPEGGDGGGRIIASGSPEEVAKVKGSYTGKYLKNIL"
     gene            complement(1369..1539)
                     /locus_tag="WIC03_000896"
     CDS             complement(1369..1539)
                     /locus_tag="WIC03_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883420.1"
                     /translation="MFLGIFIALSGILTLLEMFNVISSDMKWGMPLMFTCLGFSIIFD
                     SFKAKQNRKQKE"
     gene            complement(1554..1751)
                     /locus_tag="WIC03_000897"
     CDS             complement(1554..1751)
                     /locus_tag="WIC03_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883421.1"
                     /translation="MEMIMTNKIKPGQNTGNNGGIYQEIGSRGGRKDNYATIKDNQKA
                     PPTQNPGSSWIQIKRTPDSSR"
     gene            complement(1755..2024)
                     /locus_tag="WIC03_000898"
     CDS             complement(1755..2024)
                     /locus_tag="WIC03_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883422.1"
                     /translation="MKIKFAHLRHPAVGGGYINFAVFDAKSTNGNHSVVLKELTTEAR
                     RNGLKIDQSALIFEQHGRTQFYGDKNLVDFLSANWYPQWTHTLTI"
     gene            2162..3103
                     /locus_tag="WIC03_000899"
     CDS             2162..3103
                     /locus_tag="WIC03_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HEZ3883423.1"
                     /translation="MEYNAIMKTIAPSHIKPLMPELNQSQRNRIIFIDFYLCFLGKIN
                     RKDLMQAFQISPAVATRDFALYCSSFSKNLRFDTKSKFYLSTEDFEPQFPHSFENAIK
                     ILNGSLIFDIDSQHQPLLPFDLPPVLINPDVLIFSKISKALSLKKPLKISYHSISSGL
                     SEKVIVPLALVNDGFRWHLRAFDRTKNRFADFVLTRIIQANILLAENIEENETINNDI
                     DWNRIVKIELVPHPNHDEAKKVIEMDFGMTNGKLELALRASNIGYVLQQWRVDCSSDH
                     HIDDPAFRLWLLDNLVLYGINNAEHLAPAFDKKRSKP"
     gene            complement(3330..3596)
                     /locus_tag="WIC03_000900"
     CDS             complement(3330..3596)
                     /locus_tag="WIC03_000900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883424.1"
                     /translation="MKRQTVNQATACVRTAHTLHVDFEVPCNSQVGCVAQPRTRCWVC
                     RLRLAAAETPEEKAKDAAEEAKEEAKDAAEDAKEAAEDAKEEAK"
     gene            3845..4174
                     /locus_tag="WIC03_000901"
     CDS             3845..4174
                     /locus_tag="WIC03_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ3883425.1"
                     /translation="MDVQKQYSFETVAVCVLPNHIHAIWTLPPDDADYSLLRRLIKTK
                     FSAYSPYTKNLSAGKQRQHERGIWQRRFYGHTVRDETDLQRCADYIHSNPIKQKMSRA
                     KTESCKK"
     gene            complement(4240..4380)
                     /locus_tag="WIC03_000902"
     CDS             complement(4240..4380)
                     /locus_tag="WIC03_000902"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883426.1"
                     /translation="MPSETGNRVSDGILFVISKRGSLNIPTLFLPFEAGRIFIGQTGA
                     NP"
     gene            complement(4409..5299)
                     /gene="sucD"
                     /locus_tag="WIC03_000903"
     CDS             complement(4409..5299)
                     /gene="sucD"
                     /locus_tag="WIC03_000903"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016655272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEZ3883427.1"
                     /translation="MSVLINKDTKVLVQGFTGKNGTFHSEQALAYGTKVVGGVTPGKG
                     GQTHLNLPVFNTMKEAVKETGADASVIYVPAPFVLDSIVEAVDSGVGLVVVITEGVPT
                     LDMLKAKRYLETNGNGTRLVGPNCPGVITPGECKIGIMPGHIHTPGRIGIISRSGTLT
                     YEAVAQTTKLGLGQSTCIGIGGDPIPGMNQIDALKLFQEDPDTDAIIMIGEIGGTAEE
                     EAAEYIQSNVSKPVVGYIAGVTAPKGKRMGHAGAIISGGKGTAEEKFAAFEKAGIAYT
                     RSPAELGTTMLEVLKTKGLA"
     gene            complement(5310..6476)
                     /gene="sucC"
                     /locus_tag="WIC03_000904"
     CDS             complement(5310..6476)
                     /gene="sucC"
                     /locus_tag="WIC03_000904"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEZ3883428.1"
                     /translation="MNLHEYQAKELLASYGLPVQGGILAHNGEEAAAAYDKLGGKFAV
                     VKAQVHAGGRGKAGGVKVVKSREEAKEVAESLIGTNLVTYQTDANGQPVNSVLVCEDM
                     YPVQTELYLGAVVDRSTRRITFMASTEGGVEIEKVAAETPEKIFKVTVDPLVGLQPCQ
                     AREVAFQLGLKDKQINEFVKLMTGAYKAFVENDFALFEVNPLAVRENGALACVDGKIG
                     IDSNALYRLPKIAELRDKSQENERELKASEFDLNYVALEGNIGCMVNGAGLAMATMDI
                     IKLKGGQPANFLDVGGGATKDRVVEAFKLILEDKSVQGVLINIFGGIVRCDMIAEAIV
                     AAVKEINVDVPVVVRLEGNNAELGAKILNESGLKLTSADGLNDAAEKIVAAVNA"
     gene            complement(6575..6862)
                     /locus_tag="WIC03_000905"
     CDS             complement(6575..6862)
                     /locus_tag="WIC03_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883429.1"
                     /translation="MNKEIVGIFFIPMGIISMCMAALWQMYVMMTETYTLNRFKDKEL
                     VWRVALLFISFSLAVYLLCPNSRKKGIVFFILGGGGAAMYLLARMWLPFSK"
     gene            complement(6987..8420)
                     /gene="lpdA"
                     /locus_tag="WIC03_000906"
     CDS             complement(6987..8420)
                     /gene="lpdA"
                     /locus_tag="WIC03_000906"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEZ3883430.1"
                     /translation="MSQYDVVVIGAGPGGYVAAIRAAQLGFKTACVDAGVNKAGNAPA
                     LGGTCLNVGCIPSKALLQSSEHFHAAQHDFAEHGITVGDVKFDAAKMIERKDAIVTKL
                     TGGVKFLFQKNKVTSLFGTASFAGKNGDAYQIEVDNKGEKTVIEAKHVIVATGSVPRP
                     LPQVAIDNVNVLDNEGALNLTEVPAKLGVIGSGVIGLEMGSVWNRVGAEVTILEAAPT
                     FLAAADQQIAKEAFKYFTKEQGLSIELGVKIGDIKSEGKGVSVAYETAAGEAKTEVFD
                     KLIVAIGRIPNTKGLNAEAVGLEKDERGFIKVDGGCRTNLPNVWAIGDVVRGPMLAHK
                     ASDEGVAVAERIAGQKPHIDFNNVPFVIYTDPEIAWVGKTEEQLKAEGVEYKKGTSGF
                     GANGRALAMGKAKGTVKVLADAKTDRILGVHMIGPVVSELVTEGVTALEFFASSEDIA
                     RIIHAHPTLSEVVHEAALAADKRALHG"
BASE COUNT         2184 a         2206 c         1911 g         2327 t
ORIGIN      
        1 tccgcctcgc cagccaaatc ggcagcggcc tgaccggcgt gatgtacgtt ttggacgaac
       61 cctccatcgg cctgcaccag cgcgacaacg accgcctgct tgccaccctc aaacgcctgc
      121 gcgatttggg caacagcgtc atcgtggtcg aacacgacga agacgccatc cgcgaagccg
      181 atttcgtcgt cgatatgggc ccgggcgcag gcgaacacgg cggcaacgta ttgattgccg
      241 acacgcccga aaacgtcgcc aaatgcgaaa actccgttac cggacaatac ctcagcggca
      301 aaaaatccat tgccgtgccg tctgaacgca cgccagtcaa tcccgaccga atgctcgtcc
      361 tcaaaggcgc gcgcggcaac aacctcaaaa acgttaccct tgagctgccg ctcggtttga
      421 ttacctgcat caccggcgta tccggcagcg gcaaatccac cctgattaac gacaccctcg
      481 ccaaaatcac cgcccgcgaa ctcaaccgcg cccaagaaga acccgcccca tacgacgaca
      541 tccgcggcct cgaacacctc gacaaagtca tcaacgtcga ccaatccccc atcggccgca
      601 ccccgcgctc caaccccgcc acctacaccg gcctgttcac ccccatccgc gaactcttcg
      661 ccggtgtacc cctctcgcgc gaacgcggct acaacgtcgg cagattctcc ttcaacgtca
      721 aaggcggccg ctgcgaagcc tgccaaggcg acggcgtcat caaagtcgaa atgcacttcc
      781 tgcccgacgt gtacgtcccc tgcgaagtct gccacggcaa acgctacaac cgcgaaacgc
      841 tcgaaatcca atacaaaggc aaaaacatca gccaagtcct cgacatgacc gtcgaagaag
      901 cccgtgaatt tttcgacgcc gtccccaccg tatcgcgcaa actgcaaacc ttgatggacg
      961 taggcttagg ctacatccgt ctcggccaat ccgccaccac cctctccggc ggcgaagccc
     1021 agcgcgtcaa actcgccttg gaactctcca aacgcgacac cggcagaacg ctctacatcc
     1081 tcgacgaacc caccaccggc ctacacttcg ccgacatcgc cctgctgctg gaagtcatag
     1141 accgtctgaa aggcaaaggc aactcgatag tgattatcga gcataatctg gatgtgatta
     1201 aaaccgccga ttggattgtg gacttagggc cagaaggcgg cgacggcgga gggaggatta
     1261 ttgcttcagg tagcccggag gaagtggcga aggttaaggg gagttatact gggaagtatt
     1321 tgaagaatat actttaaaaa acgggatggt ttaaaccatc ccgttttttt attccttctg
     1381 ttttcgattt tgtttggctt tgaagctatc aaaaataatg ctaaatccca aacaggtgaa
     1441 cattaaaggc atcccccatt tcatatccga agaaataaca ttaaacattt ctaataatgt
     1501 aagaatgcct gataaagcta taaaaatacc taaaaacatt agaactatcc ttattatctg
     1561 gagctatctg gagttctttt aatttgaatc catgagcttc ctggattttg agtgggtggt
     1621 gctttttgat tatctttaat ggttgcgtag ttatcttttc gaccacctcg tgaaccaatt
     1681 tcttgataaa ttccaccgtt atttcctgta ttttgaccag gtttgatttt gttcgtcatt
     1741 atcatctcca taatttaaat agttaaagta tgagtccatt gcggatacca atttgcagat
     1801 aaaaaatcta ctagattttt gtctccatag aattgtgtac gcccatgttg ttcaaatatt
     1861 aacgcagatt ggtcaatttt taatccattt ctacgagctt cagtggtcaa ttccttcagt
     1921 acgacactat ggttaccatt agttgattta gcatcaaaaa cagcaaagtt aatatatcct
     1981 ccacctacgg ctggatgtct taagtgagca aattttattt tcataaataa atcctataaa
     2041 aaattacata acagaaattt taagtcagag agctactatg aagtagcttt ttgcattata
     2101 tgcactcttt ttgattacgt caagcaattt ttttgaattc atattgccat gaaaaagatt
     2161 gatggaatat aatgcaatta tgaaaacaat cgctccttcc catataaagc cccttatgcc
     2221 agagttgaac caatctcaac gcaataggat tatatttatt gatttctatc tatgctttct
     2281 aggaaagata aatagaaaag atttgatgca ggcatttcaa atttccccag ctgttgctac
     2341 tagggatttt gcgctatatt gctctagttt ttcgaaaaat ctccgttttg ataccaaaag
     2401 taaattttac ctctctactg aagactttga acctcaattc ccccattcat ttgaaaatgc
     2461 aattaaaatt cttaatggca gtttaatatt tgatattgat tcccaacatc aacctcttct
     2521 tccgtttgat ttacctcctg ttttaataaa tcctgatgtc ttaatctttt ctaaaatatc
     2581 gaaagcatta tccttaaaaa aacctctaaa aatctcatat cattcaattt catctggatt
     2641 atctgagaaa gttattgtgc ctcttgctct agttaatgac ggatttcgat ggcatttacg
     2701 ggcattcgat agaacaaaaa accgatttgc agattttgtc ctaacacgaa taattcaagc
     2761 aaatatttta ttagctgaaa atattgaaga aaatgaaacg attaacaatg atattgattg
     2821 gaatagaatt gttaaaatcg agttggttcc ccatcctaat catgatgaag ctaaaaaagt
     2881 gattgaaatg gactttggaa tgacaaatgg aaaattagaa ttggcgctta gagcttcaaa
     2941 tatcggatat gttttacagc aatggcgagt cgattgttcg tcagatcatc atatcgatga
     3001 tcctgctttt agattatggc tgttagataa tttagtttta tacggcatca acaatgctga
     3061 acacttagcg cctgcctttg ataaaaaacg tagcaagccg tagcctacat atccaaccga
     3121 ccgcgtgcgt ggctgcgcca cacacgcggt tgtttgattg atggctttct gttgtaaatt
     3181 gaacttagtt aaaactaaca ctttcagacg gcatgaaaaa tggccgtctg aaaactgaaa
     3241 aagctttatc taaaacaact accctaactc acaaaacaaa aaacggattc ccaatcattg
     3301 gaatccgttt ttctgtaaag ctatccgaat tatttagctt cttctttagc gtcttcagca
     3361 gcttctttgg catcttccgc tgcgtctttt gcttcttctt tagcctcttc agcggcgtct
     3421 ttggcttttt cttcaggagt ttctgcagca gcaagccgta gcctgcatac ccaacaccgc
     3481 gtgcgtggct gcgccacaca ccctacctgt gaattacacg gaacttcaaa atcaacgtgt
     3541 agggtgtgtg cggtacgcac gcacgcggtt gcttgattga cggtttgccg tttcaacttt
     3601 taacttcgtt gaagctagcg ctttcagacg gcctccaatg tcgtctgaaa gcgctagctt
     3661 caacgaagtt aaaaacctta aacaacaagc cattccttct ccaacgctca aaccaaaaac
     3721 aaaaatccta tggcgcgtta tcgcagaaac ttcattgccg gcggcacatt ctttttcacc
     3781 gtcaaactcg ccgacccgaa atcgcgcctg cttgtcgaac atatcgactt cgcgggcggc
     3841 ttatatggat gtgcaaaaac aatattcctt tgaaaccgtc gccgtatgcg tgctgccgaa
     3901 ccatattcac gccatttgga cgctgccgcc cgacgatgcg gattattccc tactccggcg
     3961 gctgattaaa accaaattct ccgcatattc cccttatact aaaaacttat ccgccggcaa
     4021 acagcggcag cacgaacgcg gtatttggca acggcgtttt tacggacaca ccgtgcgcga
     4081 cgaaacggat ttgcagcgtt gcgcggacta catccattcc aatcctatca aacaaaaaat
     4141 gtcgcgtgct aaaacagaat cctgcaaaaa ataatatcag tcttttcaaa tgcgatagtt
     4201 atcattcccc aacttcaaac aaggcgtgtg caacctgact tacgggtttg ctcctgtttg
     4261 accgataaat attctgcctg cctcaaaggg caaaaataat gtgggtatgt tgaggctgcc
     4321 tctttttgaa atgacaaaca aaatgccgtc tgaaacccga tttccggttt cagacggcat
     4381 ttgatgttca atcagttgtc aaacctgatt atgccaaacc ttttgttttc aacacttcca
     4441 gcatggtagt gcccaactcg gcagggctgc gggtgtaagc gataccggct ttttcaaacg
     4501 cggcgaattt ttcctcggca gtacctttgc cgccggaaat aatcgcaccg gcgtggccca
     4561 tgcgtttgcc tttaggagcg gtaacaccgg cgatgtagcc gacaacaggt ttgcttacgt
     4621 tggattggat gtattcggct gcttcttctt ccgcagtacc gccgatttca ccgatcatga
     4681 tgatggcgtc ggtatctggg tcttcttgga aaagtttcag tgcgtcgatt tggttcatac
     4741 cgggaatcgg gtcgccgccg ataccgatac aggttgattg acccaagccc agtttggtgg
     4801 tttgtgccac ggcttcgtaa gtcaatgtac cggaacggga aataataccg atgcggcccg
     4861 gagtgtggat gtggcccggc ataataccga ttttgcactc gcctggggta atcacgcccg
     4921 ggcagttagg gccgaccaag cgggtaccgt tgccgttggt ttccaagtaa cgtttggctt
     4981 tgagcatgtc cagagtcggc acgccttcgg taatcacaac caccaagcct acgccggaat
     5041 caacggcttc aacgatagaa tccaacacaa acggagcggg aacgtaaatt acagatgcat
     5101 ccgcgccggt ttctttaacg gcttctttca tggtgttgaa cacgggcagg ttcaggtggg
     5161 tttgaccgcc tttgcccggg gtcacgccgc cgacaacttt agtgccgtaa gccagagctt
     5221 gttcggagtg gaaagtaccg tttttaccgg tgaaaccttg aaccaatact ttggtgtctt
     5281 tattaatcaa tacgctcatt cttttctcct taggcgttta cggctgcgac aattttttcg
     5341 gctgcgtcat tcaggccgtc tgcagaagtc agtttcagac ctgattcgtt caggattttc
     5401 gcgccgagtt cggcgttgtt gccttccaaa cgaacaacga caggaacgtc gacgttgatt
     5461 tctttaacgg ctgccacgat ggcttccgca atcatgtcgc aacgtacgat accgccgaag
     5521 atgttgatca atacgccttg aacggatttg tcttccagaa tcagtttgaa cgcttcaacc
     5581 acgcggtctt tggttgcgcc gccgccaacg tccaagaagt tggcaggttg gccgcctttc
     5641 agtttgatga tgtccatagt ggccatcgcc aaaccggcac cgttcaccat acagccgatg
     5701 ttgccttcca gggcaacata gttcaggtca aattcagaag ctttcaactc gcgttcgttt
     5761 tcttgagatt tgtcgcgcaa ttcggcgatt ttcggcaggc ggtagagcgc gttgctgtcg
     5821 atgccgattt tgccgtccac gcaggcgagc gcgccgtttt cgcgaactgc cagcgggttt
     5881 acttcaaaca gggcgaagtc attttcgaca aacgctttgt acgcaccggt catcagtttg
     5941 acgaactcgt tgatttgttt gtctttcaag cccagttgga acgcaacttc gcgagcttgg
     6001 caaggttgca ggccgaccag cggatcaacg gttactttga agattttttc aggagtttcg
     6061 gcagcaactt tttcgatttc cacgccgcct tcggtagagg ccatgaatgt aatgcggcgg
     6121 gtagaacggt caaccactgc gcccaagtac agctcggttt gaaccggata catgtcttcg
     6181 caaaccaaaa cactgttgac aggttggccg ttggcatcgg tttggtaagt taccaagttg
     6241 gtgccaatca ggctttcagc cacttcttta gcttcttcgc ggcttttaac gacttttacg
     6301 ccgcccgctt taccgcggcc gccggcgtgt acttgtgctt tgacaacagc gaatttgccg
     6361 cccaatttgt cgtaagctgc agcggcttct tcgccgttgt gtgccaaaat accgccttgt
     6421 acgggcaaac cgtagctagc cagcagttct ttagcctgat actcgtgtaa attcatggat
     6481 ttctccttta gattagtgga atgccccgag tctgcctcca aatggcaagc ccgtgcgttt
     6541 cagactacct ttcggcggtc ggaaaatcgt cgtttcactt gctgaaaggc aaccacatcc
     6601 gcgccagcag atacatggct gcaccgcctc ccccgagaat aaaaaagacg atgccttttt
     6661 tacgcgaatt cggacagagc agataaacgg caaggctgaa actgataaac aacaatgcca
     6721 cgcgccaaac caattcttta tctttgaaac ggttgagcgt ataagtttcg gtcatcatca
     6781 catacatctg ccacaatgcg gccatacaca tgctgatgat gcccatcggt ataaagaaaa
     6841 taccgactat ttctttgttc ataacgaatg taaatgctgt ttgtttgtct gacggaatta
     6901 taaatcagaa aacggagctc tcggaacatt caatttgtcg aaggcattaa ggtcgtctga
     6961 aaaatttcag acgaccttaa tgtctatcaa ccgtgcaaag cgcgtttgtc ggccgccaat
     7021 gcagcttcgt gaaccacttc ggacaaggtt gggtgggcat ggataatgcg ggcgatgtct
     7081 tcgctgctgg caaagaattc gagcgcagtc acgccttcgg taaccaattc gctgacaacc
     7141 gggccaatca tgtgtacgcc caagatgcgg tcggttttgg catctgccaa tactttaacc
     7201 gtacctttgg ctttgcccat tgccaatgcg cgaccattcg caccaaaacc tgaagtacct
     7261 tttttgtact ccacgccttc ggctttgagc tgctcttcgg ttttacccac ccaagcgatt
     7321 tcaggatcgg tgtaaatcac gaacggtacg ttgttgaagt cgatatgcgg tttttgaccg
     7381 gcaatgcgtt cggcaacggc aacgccttcg tcgctggctt tgtgtgccaa catcgggccg
     7441 cgaaccacgt cgccgattgc ccatacgtta ggcaggttgg tacggcatcc gccatctact
     7501 ttgataaagc cgcgctcgtc tttttccaag cctacggctt ccgcgttcag gcctttggtg
     7561 tttggaatac ggccgatggc aacgatcagt ttgtcgaata cttcggtttt ggcttcgccg
     7621 gcagcagttt cgtaagcaac ggaaacacct ttgccttcag acttgatgtc gccgattttc
     7681 acgcccaatt cgatgctcag accttgctct ttggtgaagt atttgaaggc ttctttggcg
     7741 atttgttggt cggcggcagc caggaaggtc ggcgcggctt caagaatggt aacttccgca
     7801 cccacgcggt tccatacgga acccatttcc aaaccaatca caccggaacc gatcacgccg
     7861 agtttggcag gtacttcggt caggttcaat gcgccttcgt tgtccaatac gttcacattg
     7921 tcgatagcga cttgtggcag cggacgcggt acggaaccgg tcgctacgat gacgtgtttg
     7981 gcttcgataa cggttttctc tcctttgtta tcgacttcga tttggtaagc atcgccattt
     8041 ttaccggcaa aggaagccgt accgaacaga ctggttactt tatttttttg gaacaggaat
     8101 ttcacgccgc cggtcagttt ggtcacgatg gcatctttgc gctcaatcat tttggccgcg
     8161 tcgaatttta cgtcaccgac agtgataccg tgttcggcaa aatcgtgttg cgcagcgtgg
     8221 aaatgttcgc tggattgcaa cagggcttta gaagggatac aacctacgtt caagcaagta
     8281 ccgcccaatg caggggcatt gcctgctttg ttaacgcctg catcgacaca ggcagttttg
     8341 aaacccagtt gtgcggcacg gatggcggca acgtatccgc ccgggcctgc accaatcact
     8401 actacatcat attgagacat cgtgtcatcc tttgctttta gcagtatctc aaaataaaaa
     8461 cgatactttt cgtttctttt ttattttgaa tgactgcgtt ttgatggttt taagaaaaag
     8521 ccgtctgaaa gtccatatct gccgattcat tcgaatcaaa tgtttcagac gacctcaatc
     8581 gttcgttgag cctattgtag cacgatacta cattacgcta catctaaa
//
LOCUS       DAWHSK010000061         8580 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.061, whole genome shotgun sequence.
ACCESSION   DAWHSK010000061 DAWHSK010000000
VERSION     DAWHSK010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8580
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.061"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(236..3088)
                     /gene="gcvP"
                     /locus_tag="WIC03_000907"
     CDS             complement(236..3088)
                     /gene="gcvP"
                     /locus_tag="WIC03_000907"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HEZ3883431.1"
                     /translation="MKLSELFNPNEFAARHLSFGDEAALLAAVDEKSMDEFVGNTVPQ
                     SIRMPSELDLPEALTEADALAKLKGIASKNMINKSYIGLGYYPTRVPNVILRNVLENP
                     GWYTAYTPYQAEIAQGRLEALLNFQQVCIDLTGFPVAGASLLDEATAAAEAMAMAHRV
                     GKVKSERFFVDERVYPQTLDVMKTRAKYFGFELVVGDFAQADEGEYFGALFQYVGKDG
                     DVQDLQDVIGRLKAKGTIVAVAADIMSLVLLKSPAELGADIALGNTQRFGVPMGFGGP
                     HAAYFAFKDEFKRSAPGRIIGVSKDASGKPALRMALSTREQHIRREKATSNICTAQAL
                     LANLAGMYAVYHGPEGVKRIANRIHALASAFADALVSDGLNVVHKVFFDTVTVDFGSK
                     EQADQVFAAALESGYNLRRVSDTQVAAAFHETSTREDLVDLYRAFTGKDTFAFADDVK
                     GRLNAELLRQDDILQHPVFNRYHTEHEMLRYLKKLEDRDLAMNRSMISLGSCTMKLNA
                     TAEMLPITWAEFSDIHPYAPEAQTVGYRELLADMENSLKAITGFDAISFQPNSGAQGE
                     YSGMLAIRRYQEAQGEAQRNICLIPKSAHGTNPATAAMLGLKVVVVDTDEHGNVNIDD
                     LKAKAEQHRDALSAIMITYPSTHGVYEEGIRDICRIIHENGGQVYMDGANLNAQIGIM
                     QPAEVGADVLHMNLHKTFCIPHGGGGPGMGPIGLKAHLAPFAPGHALTDTHSASADQT
                     AVAAAAFGSASILPITWMYLTMMGKQGMEQATRWALLNANYVAKRLGEDYPILYTGKN
                     GRVAHECIVDLRPLKAESGITETDIAKRLMDYGFHAPTVSFPVAGTLMIEPTESESKA
                     ELDRFIAALKQIKQEVLKVGRGEWPKDDNPLVNAPHTAADITGNWAHPYSREEAVFPL
                     PFVREHKFWPFVNRVDDVYGDRNLVCSCPPMENYED"
     gene            complement(3119..3478)
                     /locus_tag="WIC03_000908"
     CDS             complement(3119..3478)
                     /locus_tag="WIC03_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883432.1"
                     /translation="MTDRPNLLWIKPGQAEIMEYCEPEEASDPYATYRRANLMAGLPL
                     FVVILVLLNIVFPLPAYPLAWLVPAGFMVLGGGFPLSLPLAVLLVLTCCILARCPPLS
                     RLLCYPCPNHPMSKNSA"
     gene            3426..3656
                     /locus_tag="WIC03_000909"
     CDS             3426..3656
                     /locus_tag="WIC03_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HEZ3883433.1"
                     /translation="MISACPGFIHSRFGRSVMPSEPAGRLFQKTPYRRNFMRLYGVGF
                     ALQTAYASRSSLVFQSVHDYINNLGADKPLFI"
     gene            complement(3649..4290)
                     /locus_tag="WIC03_000910"
     CDS             complement(3649..4290)
                     /locus_tag="WIC03_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HEZ3883434.1"
                     /translation="MPSEKPMNRRTFLLGAGALLLTACGRKSARTHAKIPEGSTVLAL
                     GDSLTFGYGANPGESYPAQLQKLTGWNIVNGGVSGDTSAQALSRLPALLARKPKLVIV
                     GIGGNDFLRKVPEEQTRANIAKIIETVQKENIPAVLVGMPHITLGALFGHLSDHPLYE
                     DLSEEYGIPLFGGAWAEILGDNNLKSDQIHANGKGYRKFAEDLNQFLRKQGFR"
     gene            complement(4280..4831)
                     /locus_tag="WIC03_000911"
     CDS             complement(4280..4831)
                     /locus_tag="WIC03_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEZ3883435.1"
                     /translation="MNYCEFAASLPENTDNPNKHYHDTQYGFPIDSDNELFERLVLEI
                     NQAGLSWTLILKKRQAFQTAFKGFDIDTVAAFDEADIERLLADAGIVRNRLKINAAIY
                     NARQIKQIQQEYGSFKNWLDAHHPRSKDEWVKLFKKHFKFVGGEIVGEFLMSTGYLKG
                     AHAESCPVYRETLKYHPKWLDAV"
     gene            complement(4835..5353)
                     /locus_tag="WIC03_000912"
     CDS             complement(4835..5353)
                     /locus_tag="WIC03_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HEZ3883436.1"
                     /translation="MRLFPIAAALTLAACGTVQSTQYFVLPDSRYIRPATQGGETAVE
                     VRLAEPLKRGGLVYQTDPYRLNTAQNHVWADTLDDMLEAALSNAFNRLDSARIFVPAS
                     RSGSTEKWTVYIDAFQGSYTGKTLISGYAVLPDGTNRPFHIETEQQGDGYAAMTAALE
                     QGLKQAAQQMVE"
     gene            complement(5353..7014)
                     /gene="pqiB"
                     /locus_tag="WIC03_000913"
     CDS             complement(5353..7014)
                     /gene="pqiB"
                     /locus_tag="WIC03_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HEZ3883437.1"
                     /translation="MTDNSPPSNGHAQARVRKNNTFLSAVWLVPLIALIAGGWLWIKE
                     IRNRGPVVTLLMDSAEGIEVNNTVIKVLSIDVGRVTRIKLRDDQKGVEVTAQLNADVS
                     GLIRSDTQFWVVKPRIDQSGVTGLGTLLSGSYIAFTPGKSDEAKDVFQVQDIPPVTAI
                     GQSGLRLNLIGKNDRILNVNSPVLYENFMVGQVESAHFDPSDQSVHYTIFIQSPNDKL
                     IHSASRFWLESGINIETTGSGIKLNSAPLPALLSGAISFDSPKTKNSKNVKSEDSFTL
                     YDSRSEVANLPDDRSLYYTAFFKQSVRGLSAGSPVEYKGLNVGVVSDVPYFDRNDSLH
                     LFENGWIPVRIRIEPSRLEINADEQSKEHWKQQFQTALNKGLTATISSNNLLTGSKMI
                     ELNDQPSSSPKLRPHTVYAGDTVIATRGGGLDDLQAKVVDLLDKFDKLPLDKTVAELN
                     GSLAELKSALKSANAALSSIDKLVGKPQTQNIPNELNQTLKELRQTLQGVSPQSPIYG
                     DVQNTLQSLDKTLKDVQPVINTLKEKPNALIFNSSSKDPIPKGSR"
     gene            complement(7007..8467)
                     /locus_tag="WIC03_000914"
     CDS             complement(7007..8467)
                     /locus_tag="WIC03_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HEZ3883438.1"
                     /translation="MGSAVGGQPLEKIFLPDTKGLFILPNLPVLHPDWVYRLFSFIMP
                     PLIFATFPMKPFAENIPHSLRGNCRDEALPPHTVDCPECGCRADVPQLDRGEAAFCPR
                     CGHKLFRVGRHPFSAPPAYAAASLILMAFAYGMTYIEVGIPGAASVLSLPEMMRLMVF
                     QDYGFLAEVMFVLTFGAPVLFLLLCLYVYAALIRKRVYPALRLATRVMVRLRQAMMVD
                     VFFVSTLVAYIKLSSVAEVRFGPAFYLMFALSVMLIRTSVSVPQHWVYFQIGRLTGDN
                     AVQTASEGKTCCSRCLYFRDSAESPCGVCGAELYRRRPKSLSISSAFLTAAVILYFPA
                     NILPIMISSNPAATEVNTILNGIAYMWDEGDRLIAAVIFSASILVPVLKIAAMSVLIA
                     SARFALPTGAKKLSHLYRITEAVGRWSMIDIFVIIILMCSFHTYAARVIPGSAAVYFC
                     LVVILTMLSAYYFDPRLLWDKRASDGIAFNETEKYD"
BASE COUNT         1918 a         2202 c         2430 g         2030 t
ORIGIN      
        1 tttgtccgtg tggaaatgac atcaaagggt gtgaataggc ttttcatctt tgaaatctgt
       61 ttgcacgatc ggatgaataa aaagtaggat gataaaaaag ctgatgtatg gatactggtt
      121 taactcattt gttttaaagt tatagtcgag atacgtgtat tggtgcaact ggtttgccgt
      181 tgatgaaaat gccgtctgaa aatgtttcag acggcatttt ttaagatatc aacagtcagt
      241 cttcataatt ttccatcggt gggcagctgc acacgagatt gcggtcgccg tacacgtcgt
      301 ccacgcggtt gacgaagggc caaaacttgt gttcgcggac gaacggcaac gggaagacgg
      361 cttcttcgcg ggaatacgga tgcgcccagt tgccggttat atctgcggcg gtgtgcggcg
      421 cgttgaccag tgggttgtcg tctttcggcc attcgccgcg cccgactttc agcacttcct
      481 gtttgatttg tttcagggcg gcgatgaagc ggtcgagttc ggctttgctt tcgctctcgg
      541 tcggctcgat catcagcgta ccggcaacgg ggaaggaaac cgtcggggcg tggaagccgt
      601 agtccatcag gcgtttggcg atgtcggttt cggtaatgcc gctttcggct ttgagcggac
      661 gcaaatcgac gatacattcg tgcgcgacgc ggccgttttt gcctgtatac aggatcggat
      721 agtcttcgcc cagccgtttg gcaacgtagt tggcgttgag caatgcccag cgtgtcgcct
      781 gttccatgcc ttgtttgccc atcatggtca gatacatcca agtaatcggc agtatggacg
      841 cagaaccgaa ggccgcagca gcaacggcgg tttggtcggc actggcgctg tgggtgtcgg
      901 tcaaagcgtg tcccggtgcg aacggagcca gatgggcttt caagccaatc ggacccatgc
      961 ccggaccgcc gccgccgtga gggatacaga aggttttgtg caggttcatg tgcaacacgt
     1021 ccgcgccgac ttcggcaggc tgcatgatgc cgatttgggc gttgaggttg gcgccgtcca
     1081 tataaacctg tccgccgttt tcatggatga tgcggcagat gtcgcggatg ccttcttcgt
     1141 acacgccgtg ggtggacgga taggtaatca tgatggcgga caaagcgtcg cggtgttgct
     1201 cggctttggc tttcaaatcg tcgatgttga cgttgccgtg ttcgtcggta tcgacgacga
     1261 cgactttcaa accgagcatg gcggcggtgg cggggttggt gccgtgggcg gatttgggaa
     1321 tcaggcagat gttgcgctgc gcttcgcctt gcgcttcctg atagcggcgg atggcaagca
     1381 taccgctgta ttcgccctgc gcgccggagt tgggctggaa ggaaattgcg tcaaagccgg
     1441 tgatggcttt caggctgttt tccatatcgg caagcaattc gcggtagccg acggtttgcg
     1501 cttcgggagc gtaagggtgg atgtcggaga actccgccca agtaatcggc aacatttccg
     1561 cagtggcatt gagtttcatg gtgcaactgc ccagcgaaat catactgcgg ttcatcgcca
     1621 agtcgcggtc ttcgagtttt ttcagatagc gcaacatttc gtgttcggtg tggtaacggt
     1681 tgaacacggg atgttgcaga atgtcgtctt gacgcagtaa ttcggcattc agacggcctt
     1741 tgacatcatc ggcaaatgcg aacgtatcct tgccggtaaa cgcgcggtac aaatcgacca
     1801 aatcttcgcg tgtcgaagtt tcatggaagg ctgccgcaac ttgagtatcg ctgacgcggc
     1861 gcaggttgta gcccgattcc aaagcagcgg caaacacttg gtctgcttgc tctttgctgc
     1921 cgaaatcgac ggtaacggta tcgaagaaga ccttatgaac cacattcagg ccgtctgaaa
     1981 ccagcgcatc ggcaaaggca gaagccagcg cgtgaatgcg gttggcgatg cgtttcacac
     2041 cttcagggcc gtggtaaacg gcgtacatac ccgccaagtt cgccagcaat gcctgcgcgg
     2101 tacaaatatt ggatgtcgct ttttcgcggc ggatgtgttg ctcgcgggtg gacaacgcca
     2161 tgcgcaaggc aggtttgcct gatgcgtctt tggatacgcc gatgatgcgg cccggggcgg
     2221 aacgtttgaa ctcgtcttta aacgcgaaat aagcggcgtg cggaccaccg aagcccatcg
     2281 gcacaccgaa gcgttgagtg ttgcccaacg caatgtccgc gcccaattca gccggagact
     2341 tcagcaaaac caaactcatg atgtcggcgg caacggccac gatcgtgcct tttgctttca
     2401 gacggccgat aacgtcctgc aagtcttgca catcgccgtc tttgccgacg tattggaaca
     2461 gcgcgccgaa gtattcgcct tcgtcggctt gggcaaaatc gccgaccacc agttcaaagc
     2521 cgaaatattt ggcgcgggtt ttcatcacgt ccaaagtctg cggatacacg cgctcgtcca
     2581 cgaagaaacg ctcggatttt accttgccca cgcggtgcgc catcgccatc gcttcggcgg
     2641 cggcagttgc ctcgtccagc aaagacgcgc ccgccacggg aaaaccggtc aaatcgatgc
     2701 acacttgttg gaagttcaac aaagcttcca aacgaccctg ggcaatttcc gcctgatacg
     2761 gcgtgtaggc ggtgtaccaa cccggatttt ccaatacgtt acgcaaaatc acgttcggca
     2821 cgcgggtcgg gtaatagcct aaaccgatat aggatttgtt gatcatgttt ttcgacgcaa
     2881 tgcctttcaa ttttgccaac gcgtccgctt cggtcaaggc ctcgggcaaa tcgagttcgg
     2941 acggcatacg gatgctttgc ggcacggtat tgccgacaaa ttcgtccatg ctcttctcgt
     3001 cgacagcggc aagcaacgcc gcttcgtcgc cgaaactcaa atggcgcgcg gcaaattcgt
     3061 tggggttgaa cagttcggac agtttcattt ctactccttc gggtttgtag ggctgccatc
     3121 aggcagaatt tttagacatc ggatgattcg ggcaagggta gcacaaaaga cgggataatg
     3181 gcggacaacg cgccagaatg cagcaggtca ggacaagcag caccgcaagc ggcagcgata
     3241 agggaaagcc gccgcccaaa accatgaaac ctgcaggcac cagccaagcc aatggatacg
     3301 ccggaagcgg aaaaacaata ttgagcagaa ccaaaatcac gacaaacagc ggcagacccg
     3361 ccatcaggtt ggcacgcctg tatgttgcgt acgggtcgga tgcttcttca ggttcgcaat
     3421 attccatgat ttcagcctgt ccgggcttta tccacagcag gttcggacgg tcggtcatgc
     3481 cgtctgaacc ggcaggccgt ctttttcaaa aaacgccata ccgccgcaac ttcatgcggc
     3541 tgtatggcgt aggttttgca cttcagacgg catatgcgtc ccgaagcagt ttggtgttcc
     3601 agtcggttca tgattatata aacaacttgg gtgcggataa acctttgttt atctaaaccc
     3661 ctgttttctc aaaaattgat tcaaatcttc ggcaaatttc cgatagcctt tgccgttggc
     3721 gtggatttgg tcggatttca gattattatc gcccaaaatt tccgcccacg cgccgccgaa
     3781 cagcggaatg ccgtattcct cggacaaatc ctcatacagc ggatgatcgc tcaaatgccc
     3841 gaacaacgcg cccagggtga tgtgaggcat gccgacgagg acggcgggga tgttttcctt
     3901 ttgcacggtt tcgatgattt tcgcgatatt ggcgcgggtc tgctcctcgg gaactttgcg
     3961 cagaaagtcg ttgccgccta tgccgacaat cacaagcttg ggtttgcgtg ccaacagcgc
     4021 gggcaggcgc gacagggctt gcgcggacgt atcgcccgat acgccgccgt tgacaatatt
     4081 ccaacccgtc agtttttgca gttgcgcggg gtaggattcg ccggggtttg cgccatagcc
     4141 gaaggtgagc gaatcgccca aggcaagtac ggtgcttcct tcgggaattt tggcgtgggt
     4201 tcgggcggat tttctgccgc aggcggtaag gagcaacgcg cctgcgccga ggaggaaggt
     4261 tcttctgttc attggttttt cagacggcat cgagccattt cgggtggtat ttcagggttt
     4321 cacggtaaac cggacagctt tcggcgtgcg cgcctttgag gtagccggta ctcatcagaa
     4381 attcgccgac gatttcgccg ccgacgaatt tgaaatgttt tttaaagagt ttgacccatt
     4441 cgtctttgct tcgcggatgg tgcgcgtcga gccagttctt gaacgagccg tattcttgct
     4501 gtatttgttt gatttgtcgc gcgttataaa tggctgcatt aattttcagg cggttgcgga
     4561 cgatgcccgc gtcggcaagc agccgctcaa tatcggcttc gtcaaatgcg gcaaccgtat
     4621 cgatgtcgaa acctttaaat gccgtctgaa acgcctgccg cttcttcaat atcagtgtcc
     4681 agctcaatcc tgcctgattg atttccaaca ccagccgctc aaacaattca ttgtcgctgt
     4741 caatcggaaa accgtattgc gtgtcgtggt aatgtttgtt cgggttatcg gtgttttcgg
     4801 gaagtgaggc ggcaaattcg caatagttca cggtttactc gaccatctgt tgcgccgcct
     4861 gtttcagtcc ctgttcgagt gcggcggtca tggcggcgta gccgtcaccc tgctgttcgg
     4921 tttcgatatg gaagggtctg ttcgtaccgt cgggtaggac ggcgtagccg ctgatgaggg
     4981 ttttgcccgt gtagctgcct tggaatgcgt cgatatagac cgtccatttt tcggtactgc
     5041 cgctgcgtga ggcaggaaca aagatgcgtg cgctgtccaa acggttgaat gcattgctca
     5101 acgccgcttc gagcatatcg tccaaggtgt ctgcccaaac gtggttttgt gcggtgttga
     5161 ggcggtaggg gtcggtttga tagaccagtc cgccgcgttt gagcggttcg gcaagacgga
     5221 cttcgacggc ggtttcgccg ccttgcgttg caggacggat gtagcggctg tcgggcaaca
     5281 cgaaatattg tgtgctttgc acagtaccgc aggcggcaag cgtcagggcg gcggcaatcg
     5341 ggaaaaggcg cattatcggc ttcctttcgg gatagggtct ttgctgctgc tgttgaaaat
     5401 cagcgcgttg ggtttttctt tcaaagtatt aattacgggt tgaacgtctt ttaaggtttt
     5461 gtccaaactt tgcagcgtat tttgtacgtc gccgtagata ggcgattgag gcgatacgcc
     5521 ttgcagggtt tgacgcaatt ctttcagggt ttggttcaac tcgttaggga tgttttgtgt
     5581 ctgcggtttg ccgaccagtt tgtcgatgga gcttagggcg gcattggcag atttaagtgc
     5641 ggacttgagc tcggcaagcg aaccgttcaa ttcggcaacc gtcttatcca aaggcagttt
     5701 gtcgaacttg tccagcaaat ccaccacttt cgcctgcaaa tcgtccaaac cgccgccccg
     5761 tgtggcaatg acggtatcgc ctgcataaac ggtatgcggt cgcagcttgg gcgaagacga
     5821 aggctgatcg ttcaactcaa tcattttgct gccggtcagc aggttgttgc tggagatggt
     5881 ggcggtcagg cctttgttta aggccgtctg aaattgttgt ttccagtgct ctttgctttg
     5941 ctcgtcggca ttgatttcca aacgggaagg ctcgatgcgg atgcgtacgg gaatccaacc
     6001 gttttcaaac aaatgcaggc tgtcgttgcg gtcgaaataa gggacatcgg aaaccacgcc
     6061 gacattcagg cctttgtatt cgacaggcga accggcagac aagccgcgta cggattgttt
     6121 gaaaaacgcg gtgtagtaca gtgagcggtc gtcaggcagg ttggcaactt cgctgcggct
     6181 gtcgtaaagc gtgaagctgt cttcgctttt aacgttttta ctgtttttgg ttttcggcga
     6241 atcaaatgaa atcgcgcccg acagcagggc aggcagaggg gcggaattga gtttgatgcc
     6301 gctgcctgtg gtttcgatat tgatgccgct ttccagccaa aaacggctgg cggaatgaat
     6361 cagtttgtcg ttggggcttt ggatgaagat ggtgtaatgc acgctttggt cggacgggtc
     6421 gaaatgcgcg ctttcgactt gcccgaccat aaagttttca tacaaaacag ggctgttgac
     6481 gttgaggatg cggtcgtttt taccaatcaa attcaagcgc agcccgcttt gcccgatggc
     6541 ggtaacgggc ggaatgtcct gcacttggaa cacgtctttt gcctcgtcgc ttttgccggg
     6601 tgtaaaggcg atgtacgaac ccgaaagcag cgtacccaaa ccggttacgc cgctttggtc
     6661 gatacgcggc ttgaccaccc aaaactgggt atcgctgcgg atgaggccgg atacgtccgc
     6721 attgagttgg gcggttactt ccacgccttt ttggtcgtcg cgcagtttga ttcgggtaac
     6781 gcgtccgaca tcgatgctca atactttgat gaccgtattg ttgacctcaa tgccttccgc
     6841 gctgtccatc aagagcgtaa ccacaggccc cctgttgcgg atttccttaa tccacagcca
     6901 gccaccggca atcagcgcaa tcagcgggac cagccagacg gcagagagga aggtgttgtt
     6961 tttgcggacg cgtgcttgag cgtgtccgtt tgaaggaggg ctgttgtcag tcatattttt
     7021 ccgtttcgtt gaaagcaatg ccgtctgaag cgcgtttgtc ccaaagcagg cgcgggtcga
     7081 aataataggc ggacagcatc gtcagaatca cgaccaggca gaaatagact gccgcactgc
     7141 ccggaatgac gcgcgcggca taagtgtgga acgaacacat caaaataata atcacaaaaa
     7201 tatcaatcat cgaccagcgg ccgaccgctt cggtgatgcg gtagaggtgc gacaatttct
     7261 ttgcgcccgt tggcaaagcg aagcgggcgg acgcaatcaa aaccgacatt gccgcaatct
     7321 tcagtaccgg caccaaaata ctcgcgctga aaataaccgc cgcaatcagc ctgtcgccct
     7381 cgtcccacat ataagcgatg ccgttaagga tggtattgac ctccgtggcg gcaggattgg
     7441 acgaaatcat aatcggcagg atattggcag gaaaatacaa aataaccgcc gccgtcagaa
     7501 acgccgacga aatactcaga cttttcggcc gtcggcggta cagttccgca ccgcacacgc
     7561 cgcaggggga ttcggcactg tcgcggaaat acaggcagcg gctgcaacag gttttgcctt
     7621 ccgatgccgt ctgaaccgca ttatcccccg tcagccgccc gatttgaaaa tacacccaat
     7681 gctggggaac cgataccgaa gtccgaatca gcataaccga cagcgcgaac atcagataaa
     7741 acgccggccc gaagcgaacc tctgccacag acgagagctt gatatacgcc accaaagtgg
     7801 aaacaaaaaa cacatccacc atcatcgcct gtctcaagcg caccatcaca cgcgttgcca
     7861 aacgcagcgc aggatacacc cgtttccgta tcagcgcggc atagacatac aggcacagca
     7921 gcagaaacag aaccggcgcg ccgaaagtca gcacaaacat cacttcggcc aaaaaaccat
     7981 aatcctgaaa caccatcagg cgcatcatct cgggcagcga aaggacggat gccgcacccg
     8041 gtatcccgac ctcgatatac gtcataccgt aagcaaacgc catcaaaatc agcgaagccg
     8101 ccgcataggc gggcggggcg gaaaaaggat gcctgcccac cctgaaaagt ttgtgtccgc
     8161 aacggggaca gaacgccgcc tcccccctgt ccaattgcgg cacatccgcg cggcagccgc
     8221 attccgggca atctaccgta tgcggcggca gggcttcgtc gcggcagttg ccgcgaaggc
     8281 tgtgggggat attttcggca aacggcttca tcgggaaagt ggcaaatata agtggcggca
     8341 ttataaagga aaaaaggcga tacacccaat caggatgcaa aaccggcagg ttcggcagta
     8401 tgaacaaacc cttggtgtcc ggcaaaaata ttttttcgag cggctgtccg ccgactgccg
     8461 aacccatttg ataaaatccc gattctgtaa agaatagtaa aatacctgct taaaaacatc
     8521 acgcaaatga tattttttcc aaaaatatgt gataataaat ttcgtttatg attctttatt
//
LOCUS       DAWHSK010000062         8462 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.062, whole genome shotgun sequence.
ACCESSION   DAWHSK010000062 DAWHSK010000000
VERSION     DAWHSK010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8462
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.062"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..188
                     /locus_tag="WIC03_000915"
     CDS             <1..188
                     /locus_tag="WIC03_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEZ3883439.1"
                     /translation="MLWGGYAQQKGRLIDSQNHLILTAPHPSPLSAYRGFFGCRHFSQ
                     ANSYLSQHGIEPINWKL"
     gene            229..747
                     /locus_tag="WIC03_000916"
     CDS             229..747
                     /locus_tag="WIC03_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEZ3883440.1"
                     /translation="MELHAGTTFDDIVKLKNTADIGDRLNKIIAQIAEANDLKGVIDV
                     TDFNDEDKLGKGKEMIDRLSRLVGIFKKLNLSSNQAEDDDLLGDAYEYLMRHFATESG
                     KSKGQFYTPAEVSRIMAKIIGISADCRPAPAFMTRPAARVRCCSKPPPKPAAKSAFTV
                     RKKMWQPRPLPV"
     gene            820..1293
                     /locus_tag="WIC03_000917"
     CDS             820..1293
                     /locus_tag="WIC03_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HEZ3883441.1"
                     /translation="MKTTGLRPSILPLPIRLIPPEKNGDYAFLLHLLKSLKPSGKGAI
                     ILPHGVLFRGNAEARIRTELLNLDLIKGIIGLPANLFYGTGIPACIIVIDKEHAQTAQ
                     FAEEGTNQVISGGSVFMIDASRGFIKDGNKNRLREQDIHKIIDTFTNLVTAVWCI"
     gene            1641..2585
                     /locus_tag="WIC03_000918"
     CDS             1641..2585
                     /locus_tag="WIC03_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEZ3883442.1"
                     /translation="MAHPDYAAFKAGHLAKFAAWHTQNDLAAIQPGRLIRKWSESLLD
                     AFKPGSLIEEYDFYQILTDYWAETLQDDVYLIAQDGWKAVKNLAEITKESDEAANLTV
                     VFEETETDKKGKAKTKRISKKYRSEVIAPELVARRYFSDGIAKLEEKQSELERLSQEL
                     ENHIEEHGGEEGALNDVLDAKGKLSAKLLKTALEESGIEEGERAVLQTTQTLMTQEKA
                     AKDAVKTQIEALNLAVFKQFGRLSEAEIKQLAVQDKWLADLQSRIENRLENSIQQLIS
                     RLNTLEDRYRSPMAELAREVEKWQSKVNAHLENMGFGG"
     gene            2727..2861
                     /locus_tag="WIC03_000919"
     CDS             2727..2861
                     /locus_tag="WIC03_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF45 domain-containing protein"
                     /protein_id="HEZ3883443.1"
                     /translation="MTHLLERHRNARFMAHMDNFLPNWQSIKQQLNALELFAQIYNLT
                     "
     gene            complement(3151..3666)
                     /locus_tag="WIC03_000920"
     CDS             complement(3151..3666)
                     /locus_tag="WIC03_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883444.1"
                     /translation="MKSKLPLILINFSLISSPLGANAAKIYTCTINGETVYTTKPSKS
                     CHSTDLPPIGNYSSERYIPPQTPEPVSSPSNGGQVVKYKAPVKTVSKPAKSNTPPPQQ
                     APSNNSRRSILEAELSNERKALTEAQKMLSQARLAKGGNINHQEINALQSNVLDRQQN
                     IQALQRELGRM"
     gene            complement(4132..6054)
                     /locus_tag="WIC03_000921"
     CDS             complement(4132..6054)
                     /locus_tag="WIC03_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ3883445.1"
                     /translation="MIEIKNLTLQRGLKVLLDKANATVNPGQRVGLIGKNGTGKSSLF
                     ALIKGEITQDGGDVSIPKNWRLASVSQETPDLDISALDYVLQGDAELQTLQTALRQAE
                     AQNDGMKQAEYHAKLEEIDAYTAPARAAKLLNGLGFSQEEHSRPVKSFSGGWRMRLNL
                     AQALICRADLLLLDEPTNHLDLETVLWLENHLASLPCTQIIISHDRDFLNAATTQTIE
                     LSQQKLTQYGGNYDFYQNERAQRLAQQQAAYVKQQAQIKHLQSFIDRFKAKATKAVQA
                     QSRMKALAKLERIAPAHLDSEFSFEFYNPEHLPNPLLKLEHADLGYEGKTVLHDITLS
                     LESGARYGLLGVNGSGKSTFIKALAGKIDLLSGSIVRSEKLNIGYFAQHQLDTIRADQ
                     NPVWHIQQLSPEVREQEIRNFLGGFNFVGDMALQKTEPFSGGEKARLALAMIIWQKPN
                     LLLLDEPTNHLDLDMRHALTLALQSFQGALIAVSHDRSLLEATTDSFLLIDKGRLKNF
                     DGDLNDYRQWRLAQENAAAAPAASAQSQSRKDTKRIEAQIRQEKARRGKPIQQKIDRA
                     EKEIARLTDIQTACEAFLAQEDAYLEANKEKLQNTLSQLAKVKTQLAQIEEVWLACQE
                     ELERIETEIEKQFAER"
     gene            complement(6131..6526)
                     /locus_tag="WIC03_000922"
     CDS             complement(6131..6526)
                     /locus_tag="WIC03_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HEZ3883446.1"
                     /translation="MMRQRGKKMLFEENPIDGQFAEYECGAGGIRLAGQSFHKPVLVH
                     KDSVCLSQCRTLSDLTPENLLSDIKPVDYLEILIIGTGAAQEFIHPKIMADFSRIGIG
                     VECMNTDSAFRTLVFLHSEGRRAWAWLQP"
     gene            6699..8006
                     /locus_tag="WIC03_000923"
     CDS             6699..8006
                     /locus_tag="WIC03_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEZ3883447.1"
                     /translation="MKPVNIGLLGLGTVGGGTAAVLRDNAEEISRRLGREIRISAVCD
                     LSEEKARQTCPSAAFVKDPFELVAREDVDVVVELFGGTGIAKDAVLKAIENGKHIVTA
                     NKKLLAEYGNEIFPLAEKQNVIVQFEAAVAGGIPIIKALREGLAANRIKSIAGIINGT
                     SNFILSEMREKGSAFADVLKEAQALGYAEADPTFDIEGNDAGHKITIMSALAFGTPMN
                     FSACYLEGISKLDSRDIKYAEELGYRIKLLGITRKTGKGIELRVHPTLIPESRLLANV
                     NGVMNAVRVNADMVGETLYYGAGAGALPTASAVVADIIDIARLVEADTAHRVPHLAFQ
                     PAQVQAQTILPMDEITSSYYLRVQAKDEPGTLGQIAALLAQENVSIEALIQKGVIDQT
                     TAEIVILTHSTVEKHIKSAIAAIEALDCVEKPITMIRMESLHD"
     gene            7999..8433
                     /locus_tag="WIC03_000924"
     CDS             7999..8433
                     /locus_tag="WIC03_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883448.1"
                     /translation="MTEPKHEMLTKEQVAARKKAKAKIRTIRIWAWVILALLALTALL
                     SQCAMSKPQAKQKIVESCVKNIPFAEKWQNDLRARGLDSNNTRLAVDYCKCMWEQPLD
                     RLSEKQIRSFGKLGAQEQLDLLGGANAFEARDKQCVADLKSE"
BASE COUNT         2127 a         2145 c         2140 g         2050 t
ORIGIN      
        1 tcatgttgtg gggtgggtat gcacaacaaa aagggaggct gatagacagt caaaatcatt
       61 tgatattgac cgcaccgcat ccgtctcctc tgtcggcata tcgcggtttt ttcggctgcc
      121 gccatttttc acaggcaaac agctatttga gtcaacacgg tatcgaaccg ataaactgga
      181 agctatgaat gccgatatag ccgttgccgc cggcgtgtta aaatcgcgat tgagctgcac
      241 gccggtacga cttttgacga catcgtcaaa ctcaaaaaca ccgccgacat cggcgaccgc
      301 ctgaataaga ttatcgccca aattgccgaa gccaacgact taaaaggcgt gatcgacgtt
      361 accgacttca acgacgaaga caaactgggt aaaggtaagg agatgatcga ccgtttgagc
      421 aggcttgtcg gcatttttaa aaagctcaac ctttcttcca accaagccga agacgacgat
      481 ttgttaggtg atgcctacga atacctgatg cgccattttg cgaccgagtc aggcaaatcc
      541 aaagggcagt tttacacgcc tgccgaagtc tcccgcatta tggcgaagat tatcggaatc
      601 agcgcagatt gccgtccagc accagcgttt atgacccgac ctgcggctcg ggttcgctgt
      661 tgctcaaagc cgccgcccaa gccggcagcc aaatcagcct ttacggtcag gaaaaagatg
      721 tggcaaccgc gtcccttgcc cgtatgaata tgattttgca caacaacgaa accgccgaaa
      781 tcaacaccgg gaacaccttg tccgattcgt ctttccgtga tgaaaacgac gggcttaaga
      841 ccttcgattt tgccgttgcc aatccgcctt atcccgcccg aaaaaaacgg cgattacgcc
      901 tttttgctgc atctgctcaa aagcctgaaa ccaagcggca aaggtgcgat tattcttccg
      961 cacggtgtgc tgtttcgcgg caatgccgaa gcgcgtattc gcacggaatt gcttaacctt
     1021 gaccttatta aaggcattat cgggctgcct gccaacctgt tttacggcac gggcattcct
     1081 gcctgcatca tcgtcatcga caaagaacac gcccaaaccg cccaatttgc cgaagaggga
     1141 acaaaccaag ttatcagcgg cggcagcgtg tttatgattg acgcatcgcg cggcttcatt
     1201 aaagacggca acaaaaaccg tctgcgtgag caagacattc acaaaatcat cgacactttc
     1261 acaaacctcg ttacagccgt atggtgcatt taagcgaaat cgcagcacaa gattacaacc
     1321 ttaatctgcc tcgctatatc gacagcggag aagtcgaaga cctgcaaaat ctcgccgcct
     1381 atctatcttt atggcggcat acctgcgcac gatatggacg cattggaagc ctattggcaa
     1441 gttttaggcc gtatgaaaaa cgagttgttt gccgaacacg atggccactt taccactata
     1501 caacggaatc gattgcaaat ctttcccact ctcaacagct taaaatcctg cgggattggt
     1561 gtggaattta gggctaatct agtacagccc caaatttaat ccactataaa atcgaaagca
     1621 gccaaatcaa agcccatata ttggcgcacc ccgattacgc cgccttcaaa gccggacacc
     1681 tggcaaagtt tgccgcgtgg cacactcaaa acgaccttgc cgccatccaa ccgggcaggc
     1741 ttatccggaa atggagcgaa agcctgctgg acgcgttcaa acccggcagc ctgattgaag
     1801 aatacgattt ctaccaaatc ctgacggact actgggcgga aaccctgcaa gacgatgttt
     1861 atctcatcgc ccaagatggc tggaaggcgg ttaaaaacct ggccgaaatc accaaagaaa
     1921 gcgatgaagc cgcgaacctg accgtcgtct ttgaggaaac cgaaaccgac aaaaaaggca
     1981 aagccaaaac caagcgcatc agcaaaaaat accgcagcga agtcatcgcc cccgagctgg
     2041 ttgcccgccg ctacttttca gacggcatcg ccaagctgga agaaaaacaa agcgagctgg
     2101 aacgcctaag ccaagaattg gaaaaccaca tagaagaaca cggcggcgaa gagggtgcgc
     2161 tgaacgacgt attggatgca aaaggcaaac tttccgccaa acttctgaaa accgcattgg
     2221 aagaaagcgg catagaagaa ggcgaacggg ctgttttaca aaccacccaa acactgatga
     2281 cgcaggaaaa agccgcgaaa gacgcagtca aaacccaaat cgaagccctg aaccttgccg
     2341 tattcaaaca attcggccga ctttccgaag ccgaaatcaa gcagcttgcc gttcaagaca
     2401 aatggcttgc cgatttacaa agtcgaatcg aaaatcgctt ggaaaacagt attcagcagc
     2461 ttatcagccg cttgaacacg ctggaagacc gctaccgcag cccgatggcc gagcttgccc
     2521 gagaagtgga aaagtggcaa agcaaagtca atgcccacct tgaaaatatg ggttttggag
     2581 gctgaaatgg cggcacagac aggctataag gcgagcgggt tttgagacct ttgcaaaatt
     2641 ccccaaaatc ccctaaattc ccaccaagac atttagggga tcgcggttcg ggtgtccgca
     2701 ccgcttaata cgtcgtcgtc cacgaactga cccatttgct cgaacgccat cgcaacgccc
     2761 gttttatggc gcatatggac aactttctcc caaactggca aagcatcaaa caacagctta
     2821 atgccttgga gttatttgca caaatatata atttaacata atatacatta tgcgaactat
     2881 cggaaacagt tgtacgtgtc cctgtggtct ttccaagtag gaaaattaaa gtatggccaa
     2941 tgcggctgaa tgtatagccc ggagcatccg cgtatccgaa ggtactgaac gacatgccct
     3001 gcatcccttt cgacgcttcc atccagccgg tattgggaaa tacgttgacc ttcaatataa
     3061 tatacaggca gccgacacac aggatatgcc gctgcttttt catcatttct tctgtcaaat
     3121 ccttggaacg gtcgatttga aaacacggct ttacatacgc cccagttccc tttgcagggc
     3181 ttgaatattt tgctgcctgt ccaatacatt gctttgtaat gcatttattt cttgatggtt
     3241 gatgttgccg ccctttgcca gacgtgcttg tgataacatt ttttgggctt cagtcagggc
     3301 tttgcgttcg ttgctcaatt ctgcttcgag aatggagcgt ctgctgttgt ttgagggtgc
     3361 ttgttgcggc ggcggcgtat tggattttgc cggcttggat actgttttga ccggggcttt
     3421 atatttgaca acctgtccgc cgtttgacgg tgatgatacc ggttcgggcg tttggggcgg
     3481 gatatagcgt tcgctgctgt agttgccgat tgggggcaaa tcggttgagt ggcagctttt
     3541 ggacggcttg gtggtgtaaa cggtttctcc gttgattgtg caggtgtaga ttttggccgc
     3601 attcgcaccc aatgggcttg aaatcaggga aaagttgatt aggattaagg ggagttttga
     3661 tttcataatg tgcttgatgc tcggataatt atttgatttt ttggtatttt ttataatgct
     3721 ttcttggcgt tttacgggct tttcttttcg gttgtttgct tttgattttc tttcggggct
     3781 ttggcagcct gcttgttctt tttcccggca ggttttttgt ttttcttttt actttctatg
     3841 tgtattttgg cttcttaact gagtttttta attttcaggc ggtatccgcc tccctgatgg
     3901 ctgctgattt taggtaaatc cgcatcggcg cacgatcctg ctggggctga tgtatatacg
     3961 cttttgccat ttgagttgca atggtatacg gaggcttgcg ccgccgcgcc ggaaagggac
     4021 aggagaccca aggcggcaaa aagtctgatg tttataccgg taaaaggcgg taataacgcc
     4081 cgaattatac cgttattggc aggcaaagat aagcaccctg cccgcgcttc tttatcgctc
     4141 ggcaaactgt ttctcgattt cagtttcaat ccgttccaat tcttcttgac aagccagcca
     4201 aacctcttcg atttgggcaa gttgtgtttt gacttttgcc agctgggata aggtattttg
     4261 caatttttct ttgtttgctt caagataagc gtcttcttgt gctaaaaatg cttcacaggc
     4321 cgtctgaata tcagtcaatc gtgcgatttc tttttcggca cggtctattt tctgctgtat
     4381 cggcttgccg cgtcgggctt tttcctgacg gatttgcgct tcgatgcgct tggtgtcttt
     4441 gcggctttgg ctttgtgcgg atgctgcggg tgcggcggcg gcgttttcct gtgccaaacg
     4501 ccattggcgg taatcgttca aatcgccgtc gaagttcttc agacggcctt tgtcgatcag
     4561 gaggaagctg tcggtagtcg cttcaagcag gctgcggtcg tgcgatacgg cgattaatgc
     4621 gccttggaaa ctttgcaacg cgagcgttaa ggcgtggcgc atatccaagt ccaaatggtt
     4681 ggtcggttcg tcaagcagca acaggttcgg cttttgccag ataatcatgg caagggcaag
     4741 gcgggctttt tctccgccgg aaaaaggttc ggttttctgt aacgccatat cgccaacaaa
     4801 attgaagccg ccgaggaaat ttcggatttc ttgttcgcgt acttcgggag aaagctgctg
     4861 aatatgccaa acagggtttt ggtcggcgcg gatggtatcg agttggtgtt gggcaaaata
     4921 gccgatattg agtttttcgg aacggacgat gctgccagag agcaaatcga ttttgcccgc
     4981 caaggctttg ataaatgtag atttaccgct gccgttgaca cccaataaac catagcgcgc
     5041 gccgctttcc agcgacaggg taatgtcgtg caaaacagtt ttgccttcgt aacccaaatc
     5101 tgcgtgttcc agcttcagca aaggattggg cagatgctcg ggattgtaaa actcaaagga
     5161 aaactcgctg tccaaatgcg cgggagcgat gcgttcgagc tttgccaaag ccttcatgcg
     5221 gctttgcgcc tgaacggctt tggtggcttt ggctttgaag cggtcgataa aggattgcag
     5281 atgtttgatt tgcgcctgct gtttgacata ggcggcttgt tgctgcgcga gacgctgcgc
     5341 gcgttcgttt tggtaaaaat cgtaattgcc gccgtattgc gtgagctttt gctgggacaa
     5401 ttcgatggtt tgggtggttg ccgcattgag gaaatcgcgg tcgtgggaaa tgatgatttg
     5461 cgtgcagggt aaagaggcaa ggtggttttc cagccacaag acggtttcca aatccaagtg
     5521 gttggtcggt tcgtccaaaa gcagcaaatc ggcgcggcaa atcagggctt gcgcaagatt
     5581 caagcgcata cgccagccgc cggaaaagga tttgacgggg cggctgtgtt cttcttgcga
     5641 aaaacccagc ccgttcaaca attttgccgc gcgggccggc gcggtataag cgtcgatttc
     5701 ttccaatttg gcatgatatt ccgcctgttt cataccgtcg ttttgcgctt ccgcctgcct
     5761 caatgccgtc tgaagggtct gtaactcggc atcgccctgc aaaacgtaat ctaaagcgga
     5821 aatatccaaa tcgggcgttt cttgggaaac ggaagcgagc cgccagtttt tcggaatcga
     5881 gacatcgccg ccgtcctgag tgatttcacc cttgattaag gcaaacaggc tcgatttgcc
     5941 cgtcccgttt ttgccgatca aaccgacgcg ctgaccggga ttgacggtag cgttggcttt
     6001 gtcgagcagg actttcaaac cgcgttgcag ggtgaggttt ttgatttcaa tcataacgga
     6061 aacatcgtcg ggcgggaaaa gcccgtattt tacctgaaag tcagtgccga tgccgtctga
     6121 aacgggaaat ttacggctga agccaagccc aagccctgcg cccttccgag tgcaggaaaa
     6181 ccaatgtcct gaatgccgaa tcggtattca tacattccac gccgattccg attcgagaaa
     6241 aatccgccat gattttggga tggataaact cctgagccgc gcccgtcccg ataatcaata
     6301 tttccagata gtcaacaggt ttgatgtcgg acaacaggtt ttccggagtc agatcggaca
     6361 aggttcggca ttgcgacagg cagaccgaat ccttatgtac aagcacgggt ttatggaaac
     6421 tttgccccgc cagccggatt ccgcccgcac cgcattcata ttccgcaaac tgtccgtcta
     6481 tcggattttc ttcaaacaac atttttttac cccgttgccg catcatctac accgaaaggg
     6541 gtgcaaaatc aggcgaattc atgtaggatt ggcagatttc atctgacccg cctgccgatt
     6601 tcagacggca tttgattcaa agtgcggcac aattatatcg gcagcggata ttttcgtctt
     6661 tcaatattta catttcagtc ggcttacaag gagacacaat gaagccagta aacatcggtc
     6721 ttttaggttt gggtacggtc ggcggcggta cggctgccgt gttgcgggac aacgcggagg
     6781 aaatttcccg tcgcttgggg cgcgaaatcc gtatttctgc cgtgtgcgat ttgagtgaag
     6841 aaaaagcccg acaaacctgc ccgtccgcag cctttgtcaa agatccgttc gaactggtcg
     6901 cacgtgaaga cgtcgatgtc gtcgtcgaat tgttcggcgg taccggcatt gccaaagatg
     6961 cggtgttgaa agccattgaa aacggcaaac acatcgttac cgccaacaaa aaactgctcg
     7021 ccgaatacgg caacgaaatc ttcccgctgg cggaaaaaca aaacgtcatc gtccaatttg
     7081 aagcggcagt agcgggcggt atcccaatca tcaaagccct gcgcgaaggt ttggcggcaa
     7141 acaggattaa atccatcgcc ggcattatta acggcaccag caacttcatc ctctccgaaa
     7201 tgcgcgaaaa aggcagcgcg tttgccgatg tactgaaaga agcgcaggca ttgggttatg
     7261 ccgaagccga tccgaccttc gacatcgaag gcaacgatgc gggccataaa atcaccatca
     7321 tgagcgcact ggcattcggc acgccgatga acttttccgc ctgctacctc gaaggcatca
     7381 gcaaactcga cagccgcgac atcaaatacg ccgaagaact tggctatcgc atcaaactgt
     7441 tgggcattac ccgcaaaacc ggcaaaggca tcgagctgcg cgtccaccct accctgattc
     7501 ccgaaagccg cctcttggca aacgtcaacg gcgtgatgaa cgccgtgcgc gtcaacgccg
     7561 atatggttgg cgaaacctta tattacggcg cgggcgcggg cgcattgccg accgcttccg
     7621 ccgtggttgc cgatatcatc gacatcgccc gcctggttga agccgatacc gcccaccgcg
     7681 taccgcatct ggcgttccaa cccgcgcaag tccaagcgca aaccatcctg cctatggacg
     7741 aaattaccag cagctactac ctgcgcgtcc aagccaaaga cgaaccgggc acgctggggc
     7801 aaatcgccgc gctgttggca caagaaaacg tgtccatcga agcactgatt caaaaaggcg
     7861 tgattgatca gaccactgcc gaaatcgtga ttctgaccca cagcacggtc gaaaaacaca
     7921 tcaagtcggc aatcgcagcc atcgaagcac tggattgcgt ggaaaaaccg attaccatga
     7981 tccgcatgga aagcctgcat gactgagccg aaacacgaaa tgctgacgaa agagcaggtt
     8041 gccgcgcgca aaaaagcaaa agccaaaatc cgcaccatcc gcatttgggc gtgggtcatt
     8101 ttggcgttgc tcgctttaac cgccctgctc tcccaatgcg cgatgtccaa accgcaggca
     8161 aaacagaaaa ttgtcgagtc ttgcgtgaag aatattccgt ttgccgaaaa atggcaaaac
     8221 gatttgcggg cccgcggttt agattcaaac aatacccgcc tcgccgtcga ctactgcaaa
     8281 tgtatgtggg agcagccttt ggacagattg agcgagaaac agattagatc cttcggcaaa
     8341 ctcggcgcac aagaacagct tgacctgctc ggcggcgcaa atgcctttga agcacgtgac
     8401 aagcagtgtg ttgccgattt gaaatcagaa taatgtggac cgataaaaaa gccgattctt
     8461 ta
//
LOCUS       DAWHSK010000063         8409 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.063, whole genome shotgun sequence.
ACCESSION   DAWHSK010000063 DAWHSK010000000
VERSION     DAWHSK010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8409
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.063"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(308..1240)
                     /gene="cysK"
                     /locus_tag="WIC03_000925"
     CDS             complement(308..1240)
                     /gene="cysK"
                     /locus_tag="WIC03_000925"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEZ3883449.1"
                     /translation="MKIANSITELIGNTPLVKLNRLTEGLKAEVAVKLEFFNPGSSVK
                     DRIAEAMIEGAEKAGKINKNTVIVEATSGNTGVGLAMVCAARGYKLAITMPESMSKER
                     KMLLRAFGAELILTPAAEGMAGAIAKAKSLVDAHPDTYFMPRQFDNEANPEVHRKTTA
                     EEIWRDTDGKVDVFVAGVGTGGTITGVGEVLKKYKPEVKVVAVEPEASPVLSGGEKGP
                     HPIQGIGAGFIPTVLNTKIYDSITKVPNEAAFETARAIAEKEGILVGISSGAAVWSAL
                     QLAKQPENEGKLIVVLLPSYGERYLSTPLFADLA"
     gene            complement(1434..2039)
                     /locus_tag="WIC03_000926"
     CDS             complement(1434..2039)
                     /locus_tag="WIC03_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883450.1"
                     /translation="MILLHLDFLSALLYAAVFLFLIFRAGMLQWFWASIMLWLGISVL
                     GAKLMPGIWGMTRAAPLFIPHFYLTLGSIFFFIGHWNRKTDGNGWQADPEHPLLGLFA
                     VSNVSMTLAFVGICALVHYCFSGTVQVFVFAALLKLYALKPVYWFVLQFVLMAVAYVH
                     RCGIDRQPPSTFGGSQLRLGGLTAALMQVSVLVLLLSEIGR"
     gene            complement(2036..2887)
                     /gene="dapF"
                     /locus_tag="WIC03_000927"
     CDS             complement(2036..2887)
                     /gene="dapF"
                     /locus_tag="WIC03_000927"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEZ3883451.1"
                     /translation="MNTLKFTKMHGLGNDFMVIDAVSQDFTPEDAPIAAWADRFRGVG
                     FDQLLVVGRSETEGVDFRYRIFNADGSEVEQCGNGARCFARFVADKGLTDKKEICVET
                     ANGVIFPKLSDNGMVTVNMGKPRFMPSEIPFVPESGEGDDACIYGVHLESGIQPVSCV
                     NMGNPHAVIVVDDVECAPVRETGSLIEPHRQFPERVNVGFMQIVSRTAIRLRVFERGV
                     GETQACGTGACAAVVAGIRLGLLDEGKTVEVILPGGTLYIEWACGGDVMMTGPAEAVF
                     EGELAYS"
     gene            3024..3320
                     /locus_tag="WIC03_000928"
     CDS             3024..3320
                     /locus_tag="WIC03_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883452.1"
                     /translation="MFRILLLLFPCAILAACARNIPASPAVSNCAHLQNRTILYFDAH
                     GKPADGAVKGGFYKIIEIQGANCILIQDFNSTGNALGGSYVPDCRKSSLFPKNI"
     gene            3334..4002
                     /locus_tag="WIC03_000929"
     CDS             3334..4002
                     /locus_tag="WIC03_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEZ3883453.1"
                     /translation="MNHDITFLTLFLLGFFGGTHCIGMCGGLSSAFALQLPPHINRFW
                     LILLLNTGRVSSYTAIGLILGLIGQVGVSLDQTRVLQNILYTAANLLLLFLGLYLSGI
                     SSLAAKIEKIGKPIWRNLNPILNRLLPIKSIPACLAVGILWGWLPCGLVYSASLYALG
                     SGSAATGGLYMLAFALGTLPNLLAIGIFSLQLKKIMQNRYIRLCTGLSVSLWALWKLA
                     VLWL"
     gene            complement(4075..6195)
                     /gene="pnp"
                     /locus_tag="WIC03_000930"
     CDS             complement(4075..6195)
                     /gene="pnp"
                     /locus_tag="WIC03_000930"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEZ3883454.1"
                     /translation="MFDKHVKTFQYGNHTVTLETGEIARQAAAAVKVSMGDTVVLVAV
                     TTNKEVKEGQDFFPLTVDYLERTYAAGKIPGGFFKREGKQSEKEILTSRLIDRPIRPL
                     FPEGFYHDIQIVAMVVSVDPEIDSDIPAMLGASAALVLSGVPFAGPIGAARVGYVNGV
                     YVLNPTKAELAKSQLDLVVAGTSKAVLMVESEAKILPEDVMLGAVVYGHDQMQVAINA
                     INEFADEVNPELWDWKAPETNEELVAKVRGIAGETIKEAFKIRQKQARSAKLDEAWSA
                     VKEALITEETDTLAANEIKGIFKHLEADVVRSQILDGQPRIDGRDTRTVRPLNIQTGV
                     LPRTHGSALFTRGETQALAVATLGTSRDEQIIDALSGEYTDRFMLHYNFPPYSTGEVG
                     RMGAPKRREIGHGRLAKRALLAVLPKPEDFSYTMRVVSEITESNGSSSMASVCGGCLS
                     LLSAGVPLKAHVAGIAMGLILEGNKFAVLTDILGDEDHLGDMDFKVAGTTEGVTALQM
                     DIKIQGITKEIMQIALAQAKEARLHILDQMKAAVAGPQELSAHAPRLFTMKINQDKIR
                     EVIGKGGETIRAITAETGTEINIAEDGTITIAATTQEAGDAAKKRIEQITAEVEVGKV
                     YEGTVVKILDNNVGAIVSVMPCKDGLVHISQIAHERVRNVGDYLQVGQVVNVKALEVD
                     DRGRVRLSIKALLDAPAREENAAE"
     gene            complement(6432..7295)
                     /locus_tag="WIC03_000931"
     CDS             complement(6432..7295)
                     /locus_tag="WIC03_000931"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEZ3883455.1"
                     /translation="MSEIGLVKIYFGKVRDLYEIDDKRMLMVASDRLSAFDVILDDPI
                     PSKGEILTQISNFWFKKLVHIMPNHFTGQTVYDVLPENEAKALEKRAVVAKKLTPVKV
                     EAIVRGYLAGSGWKDYQKTGSVCGIQLPEGMQEAQQLPEVIFTPSTKAAVGDHDENIS
                     FEECERIIGKELAAEVRAKAIQLYTEAAEYAKSRGIIICDTKFEFGLDTNGTLTLMDE
                     VLTPDSSRFWPADQYKVGTNPPSFDKQFVRDWLEQSGWNKKAPAPKVPADVIQKTVEK
                     YREALTLLTQD"
     gene            7495..8358
                     /gene="rfbD"
                     /locus_tag="WIC03_000932"
     CDS             7495..8358
                     /gene="rfbD"
                     /locus_tag="WIC03_000932"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEZ3883456.1"
                     /translation="MRILLTGSKSQLARCLRDRLPEDWETIATDSASLDITDADAVCN
                     MVKSFQPDAIVNTAAYTAVDKAEGNAAAAFAVNASAVYNLALAAHRAHARFIHISTDY
                     IFDGKGKRPYQESDFTNPSNVYGQSKTAGELLALSANPDSLILRTSWLFSEYGDNFVR
                     TMLNLARERSPLSAVHNQIGCPTYAGDLAAAIIRLLQQTSPVRGIYHYAGGKSVSWYE
                     FAQHIFQAASQQQTSFPVPELTAVSDKEYPTAAPRPAYSILDCRKIENDFGIKPSDWQ
                     KALAQVVSKLL"
BASE COUNT         2007 a         2419 c         1948 g         2035 t
ORIGIN      
        1 gtggattaac aaaaaccagt acagcgttgc ctcgccttgc cgtactatct gtactgtctg
       61 cggcttcatt gccttgtcct gatttttgtt aatccactat atttgtactg tctgcggctt
      121 cgttgccttg tcctgatttt tgttaatcca ctataaaaaa tatttccctc aagcgatgca
      181 gcgacaccaa cacctgatta aaacacaacc ggcgttttcg gcatatacgc aaaaacgcat
      241 tttccggcac ggcgttacac cgataccgaa aaatgcgtct gaatgtttcg acaatccgat
      301 taaagcatta tgccaaatct gcaaaaagtg gcgtagagag atagcgttcg ccataagaag
      361 gcagcagcac gactatcagc ttgccttcgt tttcaggctg tttggcaagc tgcaacgcac
      421 tccaaaccgc cgcaccggaa gaaataccca ccaaaatgcc ttctttttcc gctattgcgc
      481 gggcggtttc aaaagccgct tcgttcggca ctttggtaat gctgtcgtaa attttggtat
      541 tcaaaacggt cggaataaag cctgcgccga tgccttgaat cgggtgcggg cctttttcgc
      601 cgccgctcaa tacgggtgaa gcttcaggct cgacggcaac cactttaact tcgggtttgt
      661 attttttcaa cacttcgccc acgccggtaa tcgtaccgcc cgtgccgacg ccggcaacga
      721 agacatcgac tttgccgtcc gtatcccgcc aaatttcctc ggcggttgtt ttgcggtgga
      781 cttcggggtt tgcctcattg tcgaactggc gcggcataaa ataagtgtcg ggatgcgcgt
      841 ccaccaagga tttcgctttg gcaatcgcgc ccgccatacc ttcggcggca ggggtcagaa
      901 tcagctccgc accaaacgcg cgcaacagca ttttgcgctc cttactcatg ctttccggca
      961 tggtaatcgc cagcttgtag ccgcgtgcgg cacataccat tgccaaaccg acacccgtat
     1021 tgccgctggt tgcttcgaca atgacggtgt ttttgttgat tttgcccgct ttttcggcac
     1081 cctcgatcat tgcttcggca atgcggtctt tgacgctgct gcctggattg aaaaattcca
     1141 gtttcacggc aacctccgcc ttcaaacctt cggtcagacg gttcagtttg accaaaggcg
     1201 tgttgccgat tagttcggtg atgctgtttg caattttcat cttctgctcc tgaataataa
     1261 ggtttcgaca gtaaggcgca atccccatag gcatcctgtc cgattgcagg ggatttgcgc
     1321 cgaaacataa acaaatttcg gatatggctt tatcctaaac ctatgccgcc taaaaaacca
     1381 atatccaaaa tttctttctt tataactttt tgttccgata cggccgcacc gtattatctt
     1441 ccaatttctg aaagcagcag caccagtacc gagacctgca tcaacgctgc cgtcaacccg
     1501 ccgagtcgca gctgcgagcc gccgaacgtt gacggcggct gccggtctat accgcagcgg
     1561 tggacatagg caaccgccat cagcacaaac tgcaacacga accaataaac cggcttcagc
     1621 gcataaagtt tgagcagtgc cgcaaacaca aacacttgaa ccgttcccga aaagcaataa
     1681 tgcaccaacg cacatattcc gacaaaagca agcgtcatcg atacattact gacggcaaac
     1741 agcccgagca gaggatgttc ggggtctgcc tgccatccgt ttccatccgt tttccggttc
     1801 caatgcccga tgaaaaaaaa tatgctgccc aaagtcaggt aaaaatgggg gatgaacaag
     1861 ggcgcggcgc gggtcattcc ccatatgccg ggcatcagct ttgcccccaa aaccgatatg
     1921 cccagccaca gcataatact cgcccaaaac cattgcaaca ttcctgcgcg gaatatcaga
     1981 aacagaaaaa ccgccgcata cagtaaggca gacaaaaaat ccaaatgcag caaaatcatg
     2041 aatacgccaa ctcgccttca aacaccgctt ccgcagggcc ggtcatcatc acatcgccgc
     2101 cgcaggccca ttcgatatat aaagtcccgc ccggcaaaat cacctctacc gttttccctt
     2161 catccaacaa ccccagacgg atacccgcca ccacagccgc acacgcgccc gtaccgcacg
     2221 cttgggtttc gcccacgccg cgctcgaaca cgcgcaaacg aatcgcggtt cggctgacaa
     2281 tctgcataaa gccgacgttg acgcgttcgg gaaactgcct gtgcggttcg ataagcgaac
     2341 cggtttcgcg caccggcgcg cattctacgt catcgaccac aatcaccgca tgggggttgc
     2401 ccatattgac gcagctgaca ggctgaatgc cggattcgag atgcaccccg taaatacagg
     2461 catcatcccc ctcgcccgat tcggggacaa acggtatttc agacggcata aacctcggtt
     2521 tgcccatatt gaccgtaacc atgccgttat cggacaattt cggaaaaata acgccatttg
     2581 ccgtttcaac acaaatttct ttcttatcgg tcaaaccctt gtctgcaaca aaacgggcaa
     2641 aacaacgcgc tccgttgccg cattgctcga cctcgctacc gtcggcattg aaaatacggt
     2701 aacggaaatc cacgccttcg gtttccgaac gcccgaccac caaaagctgg tcgaagccca
     2761 cgccccggaa gcggtccgcc catgccgcaa tcggcgcgtc ctcgggggta aaatcctgac
     2821 tgaccgcgtc aatcaccata aaatcgttgc ccaaaccgtg cattttggta aatttcaaag
     2881 tgttcatatt gttccgaaat cccgtatcgt ctgttgcttc cgaatccgaa atcccccgca
     2941 ccgaagggaa atgcttccat ctcccgccgc ctgaggatta aaatcagcca ttatactgtt
     3001 tgttacccct tacaagaaac accatgttcc gcatattact gttgcttttc ccttgtgcaa
     3061 tccttgccgc ctgcgcccga aacataccgg caagccccgc cgtttcaaat tgcgcgcatc
     3121 ttcaaaaccg caccatcctg tattttgacg cacacggcaa acccgccgac ggcgcagtaa
     3181 agggcggatt ttacaaaatt atcgaaatac agggtgcaaa ctgcatcctt attcaagact
     3241 ttaacagcac gggcaatgcc ctcggcggat cctatgtgcc ggactgccgc aaatcatccc
     3301 ttttcccgaa aaatatttga cttcaaactg cctatgaacc acgacatcac tttcctcacc
     3361 ctgttcctac tcggtttctt cggcggaacg cactgcatcg gtatgtgcgg cggattaagc
     3421 agcgcgtttg cgctccaact ccccccgcat atcaaccgat tttggctgat cctgctgctt
     3481 aacacaggac gggtaagcag ctatacggca atcggcctga tactcggatt aatcggacag
     3541 gtcggcgttt cactcgacca aacccgcgtc ctgcagaata ttttatacac ggccgccaac
     3601 ctcctgctgc tctttttggg cttatacttg agcggtattt cttccttggc ggcaaaaatc
     3661 gagaaaatcg gcaaaccgat atggcggaac ctgaacccga tactcaaccg gctgttaccc
     3721 ataaaatcca tacccgcctg ccttgcggtc ggaatattat ggggctggct gccgtgcgga
     3781 ctggtttaca gcgcgtcgct ttacgcgctg ggaagcggta gtgcggcaac gggcgggtta
     3841 tatatgcttg cctttgcact gggtacgctg cccaatcttt tggcaatcgg cattttttcc
     3901 ctgcaactga aaaaaatcat gcaaaaccga tatatccgcc tgtgtacggg attatccgta
     3961 tcattatggg cattatggaa acttgccgtc ctgtggctgt aagccccatt cattcccgat
     4021 acccgtaaaa aataccgtct gaaacctgtt cagacggcac tttcacccta agcgttactc
     4081 ggcggcattt tcctcacggg caggcgcgtc cagcagggct ttgatggaca gacggacacg
     4141 gcctctgtcg tccacttcca atgctttcac gttcaccacc tgaccgactt gcaggtagtc
     4201 gccgacattg cgtacgcgct cgtgggcgat ttggctgatg tgtaccaaac cgtctttgca
     4261 cggcatcacg ctgacaatcg cgccgacgtt gttgtcgagg attttcacca cagtgccttc
     4321 atacactttg cccacttcca cttcggcagt aatctgctcg atgcgttttt tcgccgcatc
     4381 gccggcttct tgagtggtcg cggcaatggt aatcgtaccg tcttcggcaa tattgatttc
     4441 cgtaccggtt tcagcggtaa tcgcacggat ggtttcaccg cccttaccga taacttcgcg
     4501 gattttgtct tggttgattt tcatcgtaaa caaacgcggc gcgtgtgcgg acaactcttg
     4561 cgggcccgca acggcggctt tcatctgatc caagatgtgc agacgcgctt ctttggcctg
     4621 tgccaaagcg atttgcataa tttctttggt aatgccttgg attttgatgt ccatttgcag
     4681 cgcggtaacg ccttcggtcg taccggccac tttaaagtcc atatcgccca agtggtcttc
     4741 gtcgcccaaa atgtcggtca ggacggcaaa tttgttgcct tccagaatca gacccatcgc
     4801 gataccggca acgtgtgctt tcaaaggcac gccggcagac agcaggctca aacagccgcc
     4861 gcagacggaa gccatagagg aagagccgtt agattcggta atttcggaga ccacgcgcat
     4921 ggtgtagctg aaatcttcag gtttcggcaa tacggccaac aatgcacgtt tagccaaacg
     4981 gccgtgaccg atttcacggc gtttcggcgc gcccatgcgg cccacttcgc cggtagagta
     5041 cggcggaaag ttgtagtgca gcataaagcg gtcggtgtat tcgccggaca gcgcgtcgat
     5101 gatttgctcg tcgcgcgaag tacccaaagt cgctacggca agggcttggg tttcgccacg
     5161 ggtaaacaat gcggaaccgt gcgtgcgcgg caatacgccg gtttggatgt tcagcggacg
     5221 gacggtgcgg gtgtcgcggc cgtcgatgcg cggttggccg tccaaaattt ggctgcggac
     5281 gacatcggct tccaagtgtt tgaaaatgcc tttgatttcg ttggctgcca gcgtgtcggt
     5341 ttcttcggta atcaaggctt cttttaccgc actccaagct tcgtccaatt tggcagaacg
     5401 cgcttgtttt tgacggattt tgaacgcttc tttaatggtt tcgccggcaa tcccgcggac
     5461 tttggcaacc agttcctcat tggtttcagg tgctttccaa tcccaaagtt ccggattgac
     5521 ttcgtcggca aattcattga ttgcattgat ggcaacctgc atttgatcgt ggccgtaaac
     5581 caccgcgccc agcatcacgt cttcgggcag gattttggct tcggattcca ccatcaacac
     5641 ggctttggaa gtaccggcga ccaccaagtc caattgcgat ttcgccaatt cggctttagt
     5701 cgggttcaaa acgtacacgc cgtttacata accgacgcgt gccgcgccga tcgggccggc
     5761 aaacggtacg ccgctcaaca ccagcgcggc agacgcgccc aacattgcag gaatatcaga
     5821 atcgatttca ggatcgacgg acacgaccat cgctacgatt tggatgtcgt ggtagaaacc
     5881 ttcagggaac agcggacgaa tcggacggtc gatcagacgg ctggtcagga tttctttttc
     5941 gctttgtttg ccttcgcgtt tgaagaaacc gccgggaatt ttgcctgcgg cgtaagtgcg
     6001 ctcgaggtaa tcgacagtca gagggaagaa gtcttggcct tctttcactt ctttgttggt
     6061 ggtaacggca accaaaacca cggtgtcgcc catagagact ttaacggcag cggcggcttg
     6121 gcgggcaatt tcgccggttt ccaaagtaac ggtgtggttg ccgtattgga aagttttaac
     6181 gtgtttgtcg aacattattg ttcctttcaa aataccgcac tgctaaaaca ctaataatgc
     6241 acactaaaat ccgaatgtgc atagttaggg tttcagaccg tgcggcaggt tgtaaacaag
     6301 ctttcagacg acatttcgga cgctgaaagc aattccaaat tataaaggca accatcatta
     6361 aaatccagta ttgatacaat aaaaaggccg tctgaaccat atttctttca gacggccttc
     6421 aaacttaaaa atcaatcctg agtcagcaaa gtcaatgctt cccgatactt ctcgacagtt
     6481 ttctgaatca catcggcagg cactttcggc gcaggggctt ttttgttcca accgctttgt
     6541 tccagccagt cgcggacgaa ttgtttgtca aaagacggcg gattggtgcc gactttgtat
     6601 tggtcggcag gccaaaaacg gctcgaatcg ggagtcaata cctcatccat cagcgtcagc
     6661 gtaccgtttg tatccaaacc gaattcaaat ttggtatcgc aaatgatgat gccgcgcgat
     6721 ttggcatatt ccgccgcttc tgtataaagc tgaatcgctt tggcgcgcac ttccgccgcc
     6781 aattcttttc cgataatgcg ttcgcattct tcaaagctga tgttttcatc gtgatcgccg
     6841 actgcggctt tggttgaggg cgtaaaaata acttcaggca gttgttgcgc ttcctgcata
     6901 ccttcaggca gttgaatacc gcaaaccgag ccggtttttt gataatcttt ccaaccgctg
     6961 cctgccagat aaccacgcac aatcgcctct actttcaccg gagtgagctt tttagccacg
     7021 acggcgcgtt tctctaaagc tttggcttcg ttttcaggca aaacatcgta aaccgtttga
     7081 ccggtaaagt ggttgggcat aatatgcacc agttttttaa accaaaaatt ggaaatctgc
     7141 gtcagaatct cccctttgct cggaatcggg tcgtccaaaa tcacatcgaa tgcagacagg
     7201 cggtcggaag cgaccatcag catacgttta tcgtcgattt catataaatc gcgcactttt
     7261 ccaaaataga tctttaccaa accaatctca ctcatttcgc ccccccctga aaatatcttg
     7321 aaaataccga cccgacaccc gacaggtttg aatcacaaac caatattcta gccgaagtcg
     7381 gcgcaaaaca atacccatgg cacaaaaaac caacccgtca accgtcggca aagttttggc
     7441 actataatac cgacagcaag tcctacaata cacttttacc aaaggaaata cctcatgaga
     7501 atcctattga caggctcgaa aagccaactg gcacgctgcc tgcgcgaccg tcttccggaa
     7561 gactgggaaa ccattgcgac ggattccgca tccctagaca ttaccgatgc cgatgccgtc
     7621 tgcaacatgg tcaaaagttt ccaacccgac gccattgtca acacggctgc ctatactgcc
     7681 gtcgacaaag cggaaggcaa tgcggcagcg gcatttgccg tcaatgcttc cgccgtttac
     7741 aaccttgcct tggcagcaca tcgcgcccat gcccgattca tccacatctc aaccgactat
     7801 atctttgacg gtaaagggaa aaggccctat caggaaagcg actttaccaa tccttccaat
     7861 gtatacggac aatccaaaac cgcaggcgag ctgctcgcac tgtctgccaa tcccgacagc
     7921 cttatcctgc ggacttcttg gctgtttagc gaatacgggg acaactttgt ccgcacgatg
     7981 ctgaaccttg cgcgggaacg ttccccgctg tccgccgtcc acaaccaaat cggctgcccg
     8041 acctatgccg gcgatttagc tgccgccatc atccgccttt tgcagcaaac gtcgcccgtg
     8101 cgcggcattt accactacgc cggcggcaaa tccgtatcct ggtatgaatt tgcccaacat
     8161 attttccaag cggcatcgca acagcagaca tccttccccg ttcccgaatt gactgccgtt
     8221 tcagacaagg aatatccgac cgccgccccc aggcccgcat acagcatttt ggactgccgc
     8281 aaaatcgaaa acgacttcgg catcaaaccg tcagactggc aaaaagccct tgcgcaggtc
     8341 gtttccaagc tgctctgatg ccgcccgcct ctccgtttcc gccgtcaagc accgccttgg
     8401 cggttttct
//
LOCUS       DAWHSK010000064         8377 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.064, whole genome shotgun sequence.
ACCESSION   DAWHSK010000064 DAWHSK010000000
VERSION     DAWHSK010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8377
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.064"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            270..5753
                     /gene="iga"
                     /locus_tag="WIC03_000933"
     CDS             270..5753
                     /gene="iga"
                     /locus_tag="WIC03_000933"
                     /EC_number="3.4.21.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgA-specific serine endopeptidase
                     autotransporter"
                     /protein_id="HEZ3883457.1"
                     /translation="MKTKRFKINAISLSIFLAYALTPYSEAALVRDDVDYQIFRDFAE
                     NKGKFFVGATDLSVKNKQGQNIGNALSNVPMIDFSVADVNKRIATVVDPQYAVSVKHA
                     KAEVHTFYYGQYNGHNDVADKENEYRVVEQNNYEPHKAWGASNLGRLEDYNMARFNKF
                     VTEVAPIAPTDAGGGLDTYKDKNRFSSFVRVGAGRQLVYEKGAYHQEGNEKGYDLRDL
                     SQAYRYAIAGTPYKDINIDQTMNTEGLIGFGNHNTHYSAEELKQALSQDALTNYGVLG
                     DSGSPLFAFDKQKNQWVFLGTYDYWAGYGKKSWQEWNIYKKEFADKIKQRDNAGTIKG
                     NREHHWNITFGTNSKIGSTAVRLANNERDANNGQNVTFEDNGTLVLDQNINQGAGGLF
                     FKGDYTVKGANNDITWLGAGIDVADGKKVVWQVKNPNGDRLAKIGKGTLEINGTGVNQ
                     GQLKVGDGTVILNQQADADKKVQAFSQVGIVSGRGTLVLNSSNQINPDNLYFGFRGGR
                     LDANGNDLTFEHIRNVDEGARIVNHNTDRASTITLTGKSLITAPQNLSVYEIRNDYDD
                     DDYYGYYSYRKPIPQGKDLYYKNYRYYALKSGGSVNAPMPENGQTENNDWVFMGYKQE
                     EAQKNAMNHKNNQRISGFSGFFGEENGKGHNGALNLNFNGKSAQNRFLLTGGTNLNGK
                     ISVTQGNVLLSGRPTPHARDFVNKSSARKDAHFSKNNEVVFEDDWINRTFKATEIAVN
                     QSASFSSGRNVSNITANITATDNAKVNLGYKNGDEVCVRSDYTGYVTCNTDNLSDKAL
                     NSFGATQINGNVNLSQNAALVLGKAALWGQIQGQGNSSVSLNQHSKWHLTGDSQVHNL
                     SLADSHIHLNNASDAQSANKYHTLKINHLSGNGHFHYLTHLAKNLGDKVLVKESASGH
                     YQLHVQDKTGEPNQEGLNLFDASSVQDRSHLSVSLANNHVDLGALRYTIKTENGITRL
                     YNPYAENRRRVKPAPSPATNTASQAQKATQTDGAQIAKPQNIVVAPPSPQANQAEEAK
                     RQQAKAEQVKRQQAEAERKSAELAKQKAEAEREARELATRQKAEQERSSAELARRHEK
                     EREAAELSAKQKVEAEREAQALAVRRKAEAEEAKRQAAELARRHEKEREAAELSAKQR
                     VGEEERRQTAQSQPQRRKRRAAPQDYMAASQDRPKRRGHRSVQQNNVEIAQAQAELVR
                     RQQEERKAAELLAKQRAEAEREAQALAARRKAEAEEAKRQAAELAHRQEAERKAAELS
                     ANQKAAAEAQALAARQQKALARQQEEARKAAELAVKQKAETERKTAELAKQRAAAEAA
                     KRQQEARQTAELARRQEAERQAAELSAKQKAETDREAAESAKRKAEEEEHRQAAQSQP
                     QRRKRRAAPQDYMAASQNRPKRRGRRSTLPAPPSPSFDSSAYAAPRALHNPDWYENDY
                     EEIPLDALEDENVSESVDTSDKQPQDNTELHEKYENDYEEIPLDALEDEDVSESVDTS
                     DKQPQDNTELHEKVETVSLQPRAAQPRAQAATQPQAQAAAQADAVSTNTNSALSDAMA
                     STQSILLDTGASLTRHIAQKSRADAEKNSVWMSNIGYGRDYASAQYRRFSSKRTQTQI
                     GIDRSLSENMQIGGVLTYSDSQHTFDQASGKNTFVQANLYGKYYLNDAWYVAGDIGAG
                     SLRSRLQTQQKANFNRTSIQTGLTLGNTLKINQFEIVPSAGIRYSRLSSADYKLGNDS
                     VKVSSMSVKTLTAGLDFAYRFKVGNLTVKPLLSAAYFANYGKGGVNVGGNSFAYKADN
                     QQKYSAGAALLYRNVTLNVNGSITKGKQLEKQKSGQIKIQIRF"
     gene            complement(5931..7133)
                     /gene="trpB"
                     /locus_tag="WIC03_000934"
     CDS             complement(5931..7133)
                     /gene="trpB"
                     /locus_tag="WIC03_000934"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEZ3883458.1"
                     /translation="MKNYHAPDEKGVFGEHGGLYVSETLIPALQELADAYKAAKNDPS
                     FWEEFHRDLKHYVGRPSPVYHAARLSEHLGGAQIWLKREDLNHTGAHKVNNTIGQALL
                     AKRMGKKRVIAETGAGQHGVASATVAARFGMTCDVYMGADDIQRQMPNVFRMKLLGAN
                     VVGVDSGSRTLKDAMNEAMREWVARVDDTFYIIGTAAGPAPYPEMVRDFQCVIGNEAK
                     EQMLEAIGRQPDVAVACVGGGSNAIGLFHPYIGEENVRLVGVEAGGLGVNTPDHAAPI
                     TSGSPIGVLHGFRSYLMQDENGQVLGTHSVSAGLDYPGIGPEHSHLHDIKRVEYTVAK
                     DDEALEAFDLLCRFEGIIPALESSHAVAWAVKNAPKMGKDQVILVNLSGRGDKDINTV
                     AKLKGIKL"
     gene            complement(7318..8007)
                     /locus_tag="WIC03_000935"
     CDS             complement(7318..8007)
                     /locus_tag="WIC03_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HEZ3883459.1"
                     /translation="MLFEGLPCLAVWCKNRLSAVLPVGGLNVSENLLEIETHPFDSVL
                     PPKAAVMMMGTFPPKEDKRAMQFHYPNFQNDMWRVYGLVFFNDAAHFQRLSEKAFDAE
                     KIKAFLRERGIASCPTVLKAAREHGNASDKFLKVVETVDLAAVLAKIPECRHICTTGG
                     KATEILLDIQGGGIKMPKTGETVPFPFAGRDLTLTRLPSTSRAYPLSLAKKAAAYRAF
                     FEMAGLCEKQL"
     gene            8204..>8377
                     /locus_tag="WIC03_000936"
     CDS             8204..>8377
                     /locus_tag="WIC03_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase"
                     /protein_id="HEZ3883460.1"
                     /translation="MKEHKARKRFGQNFLQDTRIISDIVNAVRPQADDVVIEIGPGLA
                     AITEPLAKKLNRLH"
BASE COUNT         2552 a         2103 c         1957 g         1765 t
ORIGIN      
        1 agaaacctaa aatcccgtca ttcccgcaca agcgggaatc caaaaacaaa aagccgcaga
       61 aatttaacgg aagaaccgaa ccccgaaaat cggcttccca ccttcgcctg aataacagaa
      121 caaaggaagc ggaaataaaa acatcgggat tcttttttta aattggcaat aaaacaccaa
      181 aatgaatgat ttacacaaca aaaaatagtc aacaccacca aaccggcgta aaatacaaaa
      241 cattatcacc cttaaaacgg taaaacctta tgaaaaccaa acgttttaaa attaacgcca
      301 tatccttatc catctttctt gcctatgccc ttacgccata ctcagaagcg gcattggtca
      361 gagacgatgt cgattatcaa atattccgtg actttgcaga aaacaaaggc aaattttttg
      421 tcggcgcaac cgatttatca gtgaaaaaca aacaaggtca aaacatcggc aacgcattat
      481 ccaacgtacc gatgattgat tttagcgttg cagatgtcaa caaacgcata gcaacggtag
      541 tcgatcccca atatgctgtc agcgtcaaac acgccaaagc agaagtccat actttttatt
      601 acggtcaata caacggtcat aatgatgtag ccgacaaaga aaatgaatac cgcgttgtcg
      661 aacaaaataa ctatgaaccc cataaagctt ggggtgcgag caacttaggt cgcctcgaag
      721 attataatat ggcgcgtttt aacaagtttg taactgaagt tgcgccgatt gcaccaactg
      781 atgccggcgg cggattggat acctacaaag ataaaaaccg tttttcctct tttgtcagag
      841 ttggcgcagg caggcaatta gtttatgaaa aaggggctta tcatcaagaa ggaaatgaaa
      901 aaggctacga tttgcgcgat ctttcacaag cctatcgtta tgccattgca ggtacacctt
      961 ataaagatat taatattgac caaacaatga ataccgaagg tttgattggc tttggtaatc
     1021 acaatacaca ctattcagca gaagaactta aacaagcact ctcgcaagat gcgttaacaa
     1081 attacggcgt gttgggcgat agcggctctc cgttatttgc ttttgacaaa caaaaaaatc
     1141 aatgggtctt tttgggaact tatgattatt gggcaggtta cggaaaaaaa tcttggcaag
     1201 aatggaatat ctataaaaaa gaatttgcag ataaaatcaa acaacgcgat aacgccggca
     1261 ccatcaaagg taatagagaa catcattgga acattacttt tggcacaaac agcaagattg
     1321 gttctacagc ggtaaggctt gccaacaatg aaagggatgc aaacaacgga cagaatgtta
     1381 cctttgaaga caacggcact ttggtattgg atcaaaacat caaccaaggc gcgggcggtc
     1441 tgtttttcaa aggcgattac acagtcaaag gtgcaaataa tgacatcact tggctaggtg
     1501 cggggattga tgttgccgac ggcaaaaaag tcgtttggca agtcaaaaat ccgaatggcg
     1561 acagattggc aaaaatcggc aaaggcacat tagaaataaa cggcacaggc gttaaccaag
     1621 ggcaattaaa agtcggcgac ggtacggtta ttctgaatca acaagccgat gccgacaaaa
     1681 aagtccaggc tttctcccaa gtcggcattg tcagcggacg cggtacattg gtattaaata
     1741 gttcaaatca gattaatccc gataacttat atttcggttt ccgtggcggt cgtttggatg
     1801 ccaatggcaa tgacttgact tttgaacaca tccgcaacgt ggatgaaggc gcgcgcattg
     1861 tcaaccacaa cacagaccgc gcctccacaa tcacattaac cggcaaatca ttaattaccg
     1921 ctcctcaaaa tctttctgtt tatgaaatcc gaaatgatta cgatgatgac gattattatg
     1981 gctattacag ctatagaaaa cccattccgc aaggcaaaga tctttattac aaaaactatc
     2041 gttattacgc cctaaaatcc ggcggcagcg tgaacgcacc gatgcccgaa aacggacaaa
     2101 cggaaaataa cgactgggtc tttatgggat ataagcaaga agaagcccag aaaaacgcga
     2161 tgaaccacaa aaacaatcag cgtattagcg gttttagcgg tttctttggc gaagaaaacg
     2221 gaaaaggaca taacggcgca ttaaacctta atttcaacgg caaaagcgcg caaaaccgtt
     2281 tcttgttaac aggcggcacg aatttaaacg gaaaaataag cgtaactcaa ggcaatgtct
     2341 tgttatcagg tcgtccaaca ccacacgcaa gagattttgt gaacaaatct tcagcccgaa
     2401 aagacgcaca tttctccaaa aacaatgaag tcgtatttga agacgactgg ataaaccgca
     2461 cattcaaagc cacagaaatt gcggttaatc aatccgcatc attctcttcc ggcagaaatg
     2521 tatccaacat taccgccaac atcaccgcaa cagacaacgc caaagtaaat ttgggttaca
     2581 aaaacggcga tgaagtttgc gtgcgctcgg actataccgg ttacgttacc tgcaacacag
     2641 acaacttatc cgataaggct ttaaacagct ttggagcgac acagattaac ggcaatgtga
     2701 atttgagtca gaatgcggca ttggtcttag gcaaggctgc attatggggg caaattcaag
     2761 ggcaaggaaa cagcagcgtc agcctaaacc aacatagcaa atggcatttg accggcgaca
     2821 gccaagtaca caatctgtca ttggcggata gccatattca tttgaacaac gcttccgacg
     2881 cgcaaagtgc aaataaatac cacacgctca aaatcaatca tttatccggt aacgggcatt
     2941 ttcattatct gacgcacctg gcgaaaaatc ttggggataa ggtgcttgtg aaggaatccg
     3001 catccggtca ttatcagctc catgttcaag ataaaacagg cgaacccaat caggaagggc
     3061 tgaatctctt tgatgcatca tccgtacaag accgttcgca cctttctgtt tccttggcga
     3121 ataaccatgt agatttaggc gcattgcgtt atacaatcaa aacagaaaac ggtattaccc
     3181 gattgtacaa tccttatgcc gagaaccgcc gccgagtcaa accggccccg tctcctgcca
     3241 caaacacggc ttctcaagca caaaaggcaa cacaaacgga cggtgcacaa attgccaagc
     3301 ctcaaaatat cgtcgtcgca ccacctagcc cgcaggcaaa tcaagccgaa gaagcaaaac
     3361 gccaacaagc aaaagcggag caagtgaagc gtcagcaagc agaagcagag cgtaaatcgg
     3421 cagagttagc aaaacaaaaa gcagaagccg aacgcgaagc aagagagctg gcgacccgcc
     3481 aaaaagcaga acaagaacgc agcagcgccg aacttgcacg tcgacatgaa aaagaacgcg
     3541 aagcggcaga gttgtcggca aaacaaaaag tcgaagccga acgtgaggct caagcattgg
     3601 cggtacgccg aaaagccgaa gccgaagagg caaaacgcca agctgcagag cttgcacgcc
     3661 ggcatgaaaa agaacgcgaa gcggcagagt tgtcggcaaa acaaagggtc ggggaagagg
     3721 aacgccgtca aacagcccaa tcccagccgc aacgccgcaa acgccgtgcc gcaccgcagg
     3781 attatatggc agcttcccaa gaccgtccta aacgccgcgg acaccgttcg gtacagcaaa
     3841 acaatgtgga aattgcccaa gcccaagcag aacttgtgcg ccgacagcag gaagaacgca
     3901 aagcggcaga gttgttggca aaacaaaggg cagaagccga acgtgaggct caagcattgg
     3961 ctgcgcgccg aaaagcagaa gccgaagagg caaaacgcca agctgccgaa cttgcacatc
     4021 gccaggaagc agaacgcaaa gcggcagaat tgtcggcaaa ccaaaaagcc gcagctgaag
     4081 cccaagcatt ggcggcacgc caacaaaaag cccttgcgcg tcaacaggaa gaagcacgaa
     4141 aagctgcaga gttagccgtc aaacaaaaag cggaaaccga acgcaaaacc gccgaacttg
     4201 ccaagcaaag ggcagcagcc gaagcagcaa aacgccagca agaagctcgc cagaccgccg
     4261 aacttgcacg tcgtcaagaa gcggaacgcc aagcagcaga gttgtcggca aaacaaaaag
     4321 ccgaaacaga tcgagaagcg gcagaatcgg cgaaacgaaa agctgaggaa gaagagcacc
     4381 gtcaagcagc ccaatcccag ccgcaacgcc gaaaacgccg tgccgcacct caggattata
     4441 tggcagcttc ccaaaatcgt ccgaaacgtc gcggacgcag atctactctg ccggcaccgc
     4501 cctcgccatc atttgattcg tcagcttacg cagcacccag ggccttgcat aatccggact
     4561 ggtatgaaaa tgactatgaa gaaatcccct tggatgcgtt ggaagatgaa aatgtatctg
     4621 aatcagttga cacatcagac aaacagcctc aagacaatac ggaacttcat gaaaaatatg
     4681 aaaatgatta tgaagaaatc cccttggatg cgttggaaga tgaagatgta tccgaatcgg
     4741 ttgacacatc agacaaacag cctcaagaca atacggaact tcatgaaaaa gttgagacag
     4801 tgagtttgca accaagagcc gcgcagccgc gagcccaagc cgccactcag ccgcaagccc
     4861 aagccgccgc gcaagccgat gcagtcagca ccaataccaa ctcggcttta tctgacgcaa
     4921 tggcaagcac gcaatctatc ttgttggata caggtgcttc attaacacgg cacattgcac
     4981 aaaaatcacg cgctgatgcc gaaaaaaaca gcgtttggat gtcgaacatc ggttatggcc
     5041 gtgattatgc ttccgcacaa tatcgccggt ttagttcgaa acgcacgcaa acacaaatcg
     5101 gcattgaccg cagcttgtcc gaaaatatgc agataggcgg cgtattgact tactctgaca
     5161 gtcagcatac ttttgatcag gcgagcggca aaaatacttt tgtgcaagcc aacctttatg
     5221 gtaagtatta tttaaatgat gcttggtatg tggccggcga tattggtgca ggcagcttga
     5281 gaagccggtt acaaacacag caaaaagcaa actttaaccg aacaagtatc caaaccggcc
     5341 ttactttggg caatacgctg aaaatcaatc aattcgagat tgtccccagt gcgggaatcc
     5401 gttacagccg cctgtcatct gcagattaca agttgggtaa cgacagtgtt aaagtaagtt
     5461 ctatgtcagt gaaaacactg acggccggac tggattttgc ttatcggttt aaagtcggca
     5521 accttaccgt aaaacccttg ttatctgcag cttactttgc caattatggc aaaggcggcg
     5581 tgaatgtggg cggtaattcc ttcgcctata aagcagataa tcaacagaaa tattcagcag
     5641 gtgccgcgtt actgtaccgt aatgttacat taaacgtaaa tggcagtatt acaaaaggca
     5701 aacaattgga aaaacaaaaa tccggacaaa ttaaaataca gattcgtttc taaaatacta
     5761 aattcatagc aaaataaaat gccgtctgaa ctcaggcttc agacggcatt tttatagtgg
     5821 attaacaaaa atcaggacaa ggcgacgaag ccgcagacag tacagataga cggcaaggcg
     5881 aggcaacgcc gtactggttt ttgttaatcc actatatcag acggacgagg ttacagttta
     5941 atccctttga gcttcgcgac ggtattgatg tctttgtcgc cacgacctga gaggttgacc
     6001 agaatcactt ggtctttacc cattttcggc gcatttttca ccgcccaagc aacggcgtgg
     6061 ctggattcga gcgcggggat gatgccctcg aagcggcaga gcaagtcaaa ggcttcgagt
     6121 gcttcgtcgt ctttggcaac ggtgtattcg acgcgcttga tgtcgtgcag atggctgtgt
     6181 tccgggccga tgccggggta atccaagcct gcggaaacag agtgtgtacc caagacttga
     6241 ccgttttcgt cctgcatcag atagctgcgg aaaccgtgca atacgccaat cggtgagccc
     6301 gaagtaatcg gcgcggcgtg atcgggggtg ttcacgccca aaccgccagc ctccacgccg
     6361 acgaggcgca cgttttcttc gccgatatac gggtggaaca aaccaatggc gttcgatccg
     6421 ccgcccacgc aggcaacggc aacgtcgggc tgtctgccga tggcttccaa catttgttct
     6481 ttggcttcat tgccgatcac gcattgaaag tcgcgcacca tttcgggata cggcgcggga
     6541 ccggcggcgg taccgatgat gtagaatgtg tcatccacgc gggcgaccca ttcgcgcatg
     6601 gcttcgttca tcgcgtcttt caaagtacgg ctgccgctgt caacgccgac gacattcgcg
     6661 cccaataatt tcatacggaa cacgttgggc atttggcgtt ggatgtcgtc cgcacccatg
     6721 tacacgtcgc aagtcatgcc gaagcgggcg gcaacagtgg cactcgccac gccgtgctga
     6781 cccgcgccgg tttcggcgat gacgcgtttt ttacccatac gttttgccaa cagtgcctga
     6841 ccgatggtgt tgttgacttt gtgcgcgccg gtgtggttca agtcttcgcg cttcaaccaa
     6901 atttgcgcgc cacccaaatg ctcggacaat cgcgcggcat ggtaaacggg gctgggcctg
     6961 ccgacgtagt gtttcaaatc gcggtggaat tcttcccaaa acgacgggtc gtttttcgct
     7021 gccttatagg catccgccag ctcttgcaag gcgggaatca gggtctcgga aacataaagc
     7081 ccaccgtgtt cgccgaaaac gcccttctcg tcgggtgcgt ggtaattctt catcagattt
     7141 cgtccttgtg cttcaaattt tcagacggca atttatagtg gattaacaaa aaccggtacg
     7201 gcgttgcccc gccttgccgt actatttgta ctgtctgcgg cttcgccgcc ttgtcctgat
     7261 ttttgttaat ccactatatt tttcaaacat gccgtccgaa cgggaaattg tcggcaatta
     7321 taactgcttt tcacacaagc ccgccatttc aaaaaacgcc cgatacgccg ccgctttttt
     7381 cgccaaactc aaaggatagg cacgcgaagt cgaaggcagg cgcgtcagcg tcaaatcccg
     7441 tccggcaaac ggaaacggca cggtttcgcc cgttttcggc attttgatgc cgccaccctg
     7501 aatatcgagc aggatttccg tcgccttgcc gcctgtcgta caaatatggc ggcactcggg
     7561 tatttttgcc aacaccgccg ccaaatcgac ggtttcaact acctttaaaa acttgtcgga
     7621 cgcattgccg tgttcccttg ccgccttcaa cacggtcgga caggacgcaa ttccccgttc
     7681 gcgcaaaaac gccttgattt tctcggcatc aaacgctttt tcagacaacc tttggaaatg
     7741 cgccgcatca ttaaaaaaca ccagcccgta aacgcgccac atatcgtttt ggaaattcgg
     7801 ataatgaaac tgcatcgcgc gtttgtcttc cttgggcgga aacgtcccca tcatcatgac
     7861 cgcagccttc ggcggcaaca cggaatcgaa gggatgggtt tcgatttcaa gcagattttc
     7921 agacacgttc aatcctccaa caggcaaaac ggcagacagc cggtttttgc accaaaccgc
     7981 caaacacggc aagccttcaa acagcattat cacgccctgc cgttttcggt tacaatgccg
     8041 acatcaaacg gatactgtaa acacatttat gttccaacac acaggacgac acttaaagca
     8101 ccgccctatg tgccatcctg atttggaagg ggttacgccc ctcccaaata gagtctgatt
     8161 ctaccgccct aaagggcggg gtttcaaccg aaaaggaaac acgatgaaag aacacaaagc
     8221 ccgcaagcgt ttcgggcaga attttttgca ggacacgcgg attatcagcg atattgtcaa
     8281 cgccgtgcgc ccgcaggcgg atgatgtcgt gattgaaatc ggtccgggtt tggcggcgat
     8341 taccgaacct ttggcgaaaa agctgaaccg cctgcac
//
LOCUS       DAWHSK010000065         8314 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.065, whole genome shotgun sequence.
ACCESSION   DAWHSK010000065 DAWHSK010000000
VERSION     DAWHSK010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8314
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.065"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..495)
                     /locus_tag="WIC03_000937"
     CDS             complement(<1..495)
                     /locus_tag="WIC03_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HEZ3883461.1"
                     /translation="MLTDLEKNAIRDHYQNIGKNLPGFRPRASQREMIAAVANAFSRT
                     LAREEGGEPPKREGESIAVIEGPTGVGKSLAYLLAGGIMAQTRGKRLIVSSATVALQE
                     QLVDRDLPFLVEKSGLELTFALAKGRGRYLCPYKLYQLTQSNAQQNLLGFEAPAVLWD
                     SKPKP"
     gene            598..822
                     /locus_tag="WIC03_000938"
     CDS             598..822
                     /locus_tag="WIC03_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883462.1"
                     /translation="MVFWLQRLKYPLLLWIADMLLYRLLGGAEIECGRCPVPPMTDWQ
                     HFLPAMGTVSAWVAVIWAYLIIESEKNGRY"
     gene            complement(890..2191)
                     /locus_tag="WIC03_000939"
     CDS             complement(890..2191)
                     /locus_tag="WIC03_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEZ3883463.1"
                     /translation="MPSETARPLYADTRAAHTLVWFRQNLRIRDNAALCAAVAEGLPV
                     IGIWIDDAETDNPRRAAFYRQSAAELAQGLAGRGIPLYTAASPAELVRLAVRLNIRAV
                     IADESHTFADKLADNALWHELDKHGIALTFVNDRAVFGKTDLIPDGGTAHTDFDRYRE
                     VWLDRFSKQPPAGPDLFAAYRQPFPENLSAPPPAALSDGIFLPQNSGETAAWRQWRRF
                     LEQADSYSVLKDFPSRKHTSLMGAYLSAGCISPRLLARESLERRLNAWADNIIRRDFF
                     LQLALQHTDDDPSDGNPEHTLRLTLWQQGRTGIPIIDAAMRCLHKTGSLHPALRRLSA
                     DFFCHVLNLPRREGEIWFARQLTDFDAAINQGNWWLAASRHTCPDIAAASYRTDPDGT
                     FIKRHIPELAHLSADTVHTPWRFACSVDTHGYPAHPVAGIP"
     gene            complement(2181..3092)
                     /locus_tag="WIC03_000940"
     CDS             complement(2181..3092)
                     /locus_tag="WIC03_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEZ3883464.1"
                     /translation="MDTLFSLKVFRQVVQSGGFTRAADALSISTAMASKHVSHLENTV
                     QAKLLHRNSRNLSLTEAGEEYYRQCSYALDTLDDAAQKAAGGTEKPQGLLRVTMPLWF
                     AGSQICNWLAEYRERYPEVTLELILDNRHVDLIAEGVDLALRVSQTLSPSLIARPLAE
                     IEFALLASPDFLRRNGVPETPEEVAGLPAVLPTYTNQQKLDLTRKSDGKKYRLELTPV
                     IRTDNTLMMREMIKAGACIGYQPLWAAEHDLRCGTLVRLLPGYAVPTDRLNAVYADRA
                     FLSAKVRSFIDFLNEKIASRKGCRNAV"
     gene            3200..3598
                     /locus_tag="WIC03_000941"
     CDS             3200..3598
                     /locus_tag="WIC03_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEZ3883465.1"
                     /translation="MSDCCNRIQPVLLSVLRIVTAYLFLLHGTSKIFAFPIEMGSGSP
                     GGLLLLAGILEIVGGILLVLGLFARPAAFVLSGQMAVAYFMAHASGNALFPIANGGES
                     AVLFCFVFLYIAAAGGGAWSLDRLFFKRKA"
     gene            3647..4252
                     /locus_tag="WIC03_000942"
     CDS             3647..4252
                     /locus_tag="WIC03_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEZ3883466.1"
                     /translation="MTRQSLQQAAESRRSIYSLNKNLPVGKDEVVQIVEHAVLHTPSS
                     FNSQSARVVVLFGEEHDKVWQFVEDALRAVVPADSFEPTAQKLNLFKAGAATILFYED
                     QNVVKGLQEQFPAYAANFPVWADQANAMVQYAVWTTLAAVGVGANLQHYNPLPDAAIA
                     KAWNIPENWLLRAQMVIGGIEGAAGEKTFEPVAERLKVFGA"
     gene            4438..6549
                     /locus_tag="WIC03_000943"
                     /pseudo
     CDS             4438..6549
                     /locus_tag="WIC03_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226965.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            6752..7411
                     /locus_tag="WIC03_000944"
     CDS             6752..7411
                     /locus_tag="WIC03_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEZ3883467.1"
                     /translation="MKTAILIDGAFFIKRVRSLGLSQNPHDAKRIADLAFRYSMLHLK
                     DKINGTDQMDDLYRIFFYDCVPFEKKMHNPVSHRAIDFSKTPEAIFRRNLHTELKKKR
                     KLALRLGKLSDNTAWILKPQVMKNLLKNPSTQITENDVVLDVKQKGVDMRIGLDISSI
                     TLKKQADKIILFSGDSDFVPAAKLARREGIDFILDPMWNHVPPDLMEHIDGLKTKCPK
                     P"
     gene            7582..8277
                     /locus_tag="WIC03_000945"
     CDS             7582..8277
                     /locus_tag="WIC03_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEZ3883468.1"
                     /translation="MKLKTLALTSLTLLALAACSKQAETSVPADSVQSSSSAPAAPAP
                     LTEGVNYTVLSTPIPQQQAGKVEVLEFFGYFCPHCAHLEPVLSEHIKTFKDDTYMRRE
                     HVVWGDEMKPLARLAAAVEMAGESDKANSHIFDAMVNQKINLADTDTLKKWLSEQTAF
                     DGKKVLAAFEASESQARAAQMEELTNKFQISGTPTVIVGGKYQVEFKDWQSGMTTIDQ
                     LVDKVREEQKKPQ"
BASE COUNT         1984 a         2138 c         2275 g         1917 t
ORIGIN      
        1 gggcttgggt ttgctgtccc acaagacggc gggggcttca aagccgagca ggttttgctg
       61 ggcattgctt tgcgtcagtt ggtagagttt gtaggggcag agatagcggc cgcgcccttt
      121 ggcaagtgcg aaggtcagtt ccaaaccgct tttttcgacc agaaacggca ggtcgcggtc
      181 taccaactgc tcctgcaagg caaccgtcgc gctgctcaca atcagccgct tgccgcgtgt
      241 ttgcgccatg atgccgcccg ccagcaggta ggcaagcgat ttgcccacgc cggtcggccc
      301 ttcgattacg gcgatgctct cgccctcgcg cttgggcggc tcgccgcctt cttcacgcgc
      361 caacgtccgc gaaaaagcgt tggcaaccgc cgcaatcatt tcccgctgcg aagcacgcgg
      421 acggaaaccg ggcaggtttt tgccgatgtt ttggtaatgg tcgcggatgg cgtttttttc
      481 taaatcggtg agcatggcgt tttgtacggc ggtagaagtg ggcttatttt aacattgcac
      541 ggaagcggta caatatcgtt gtcggaatgg ggggtgaggt gaatcgtgcg gacgtggttg
      601 gttttttggt tgcagcgttt gaaatacccg ttgttgcttt ggattgcgga tatgctgctg
      661 taccggttgt tgggcggcgc ggaaatcgaa tgcggccgtt gccctgtgcc gccgatgacg
      721 gattggcagc attttttgcc ggcgatggga acggtgtcgg cttgggtggc ggtgatttgg
      781 gcatatctga ttattgaaag tgaaaaaaac ggaagatatt gagtcatccg gacgcaatgc
      841 cgtctgaaac ggaagttcag acggcatttg ttttaggttg ccgtaccgct tagggaatac
      901 cggcgacagg atgggcggga tagccgtggg tatcgaccga acaggcaaac cgccaaggcg
      961 tgtggacggt gtcggcggac aggtgggcga gttcgggaat gtgccgtttg ataaaagtac
     1021 cgtccgggtc ggtacggtat gaggcggcgg caatgtcggg gcaggtgtgc cgtgaggcgg
     1081 caagccacca gttgccttgg ttgattgccg catcgaaatc ggtcagctgc cgggcaaacc
     1141 atatctcgcc ttcgcggcgg gggaggttta aaacgtggca gaaaaaatcc gcgctcaagc
     1201 gtctcagggc ggggtggagg ctgccggttt tgtgcaaaca gcgcatcgcg gcatcgataa
     1261 tcggaatgcc ggtccggccc tgctgccaaa gcgtcaggcg cagggtgtgt tcaggattgc
     1321 cgtctgaagg gtcgtcatcc gtgtgctgca aggcaagttg aaggaaaaaa tcgcggcgga
     1381 tgatgttgtc cgcccacgcg ttcagacggc gttcgaggct ttcccgcgcg agcaggcgcg
     1441 gcgagatgca gccggcactc aaatacgcgc ccatcagcga agtgtgtttg cgcgagggga
     1501 aatcctttaa aacggagtag gaatccgcct gttcgagaaa ccgccgccac tgccgccaag
     1561 ccgccgtttc gccgctgttt tgcggcagga agatgccgtc tgaaagggcg gcaggcggcg
     1621 gggcggaaag gttttcgggg aagggttggc ggtatgccgc gaataggtcc ggaccggcgg
     1681 ggggctgctt ggaaaagcgg tcgagccata cttcgcggta gcggtcgaaa tcggtgtgtg
     1741 ccgtgccgcc gtcgggtatc aggtcggttt tgccgaaaac ggcgcggtca ttgacgaagg
     1801 ttaacgcgat gccgtgtttg tccaattcat gccaaagggc gttgtcggcg agtttgtcgg
     1861 caaaagtatg ggattcgtcg gcgatgacgg cgcggatatt gaggcggacg gcgagccgga
     1921 cgagctcggc aggcgatgcc gccgtgtaga gcgggatgcc gcgccctgca agcccttggg
     1981 cgagttcggc ggcggattgg cggtagaacg cggcgcggcg agggttgtct gtttcggcat
     2041 cgtcaatcca aataccgata acgggcaaac cttcggcaac ggcggcgcat aaggcggcgt
     2101 tgtcgcggat gcggaggttt tggcggaacc agacgagcgt gtgtgcggcg cgcgtgtccg
     2161 cataaagggg gcgggcggtt tcagacggca tttcggcagc ctttcctgct ggcgattttt
     2221 tcgttcagaa aatcgatgaa gctgcggact ttcgcgctta agaatgccct gtccgcataa
     2281 acggcgttca gccggtcggt cgggacggcg tatccgggca gcagcctcac cagcgtgccg
     2341 cagcgcaaat cgtgttccgc agcccaaagc ggctgataac cgatgcacgc gcccgcctta
     2401 atcatttcgc gcatcatcag cgtgttgtcg gtacggatga cgggggtcag ttcaagccgg
     2461 tattttttgc cgtccgattt gcgggtgagg tcgagtttct gctggttggt gtaggtcggc
     2521 aggacggcgg gcagccccgc cacttcttcc ggcgtttccg gtacgccgtt gcgcctcagg
     2581 aaatcgggcg aggcgagcag ggcaaattcg atttccgcca gcgggcgcgc aatcagcgac
     2641 ggggacaggg tttgggaaac gcgcaacgcc aaatccacgc cttcggcaat caaatcgacg
     2701 tggcggttgt ccaaaatcag ttctaatgtc acttcgggat aacgttcgcg gtattccgcc
     2761 agccagttgc atatctggct gccggcaaac cacagtggca tcgttacgcg cagcagcccc
     2821 tgcggttttt ccgtcccccc ggcggctttt tgcgcggcat cgtcgagcgt gtcgagcgcg
     2881 taactgcatt gccggtagta ttcttccccg gcttcggtca ggctgaggtt gcggctgttg
     2941 cggtgcagga gtttggcttg gacggtgttt tccaagtggc tgacgtgttt gcttgccatt
     3001 gcggtggaga tgctgagcgc gtcggcggcg cgggtgaagc cgccgctttg gacgacttgg
     3061 cggaaaacct tgaggctgaa cagggtgtcc atattttctt gtgtggaaaa gttgtatcaa
     3121 taaaagcagt atatatttga aaaggggaaa catctatact ctaccgcctg aaatgaagac
     3181 aaatatcaaa ggagctttta tgtccgattg ctgcaaccgt atccaaccgg ttttgctttc
     3241 tgttttgcgt atcgtaaccg cttacctgtt tttgttgcac ggtacgtcga aaatcttcgc
     3301 cttccccatt gaaatgggca gcggttcgcc cggcgggctg ttgctgcttg ccggtatttt
     3361 agaaattgtc ggcggcattt tgctggtgtt gggcctgttt gcgcgccctg ccgcgtttgt
     3421 tttgtccggc cagatggcgg ttgcctattt tatggcgcac gcttccggaa atgctttgtt
     3481 cccgattgcc aacggcggcg agtccgcagt gctgttctgc ttcgtattcc tctatatcgc
     3541 ggcggcgggc ggcggagcat ggtcgctgga caggctgttt ttcaagcgta aagcctgaat
     3601 cggactgcct aaagtgtatt ttgttgaatg tttttgagga aaagaaatga cccgtcaatc
     3661 tctgcaacag gctgccgaaa gccgccgttc catttattcg ttaaataaaa atctgcccgt
     3721 cggcaaagat gaagttgtcc aaatcgtcga acacgccgtt ttgcacacac cttcttcgtt
     3781 caattcccaa tctgcccgcg tggtcgtgct gtttggcgaa gagcatgata aggtgtggca
     3841 atttgtcgaa gacgcgctgc gtgccgtcgt gcctgccgac agttttgaac cgaccgcgca
     3901 aaaattgaac ctgtttaagg cgggtgcggc aaccattttg ttttatgaag atcaaaatgt
     3961 cgtcaaaggt ttgcaggagc agttccctgc ttatgccgct aacttccccg tttgggcgga
     4021 ccaggcaaac gcgatggtgc agtatgccgt ttggacgaca cttgccgcgg tcggcgtagg
     4081 tgcaaacctg caacattaca atcccttgcc cgatgcggcg attgccaaag cgtggaatat
     4141 ccccgaaaac tggttgttgc gcgcacaaat ggttatcggc ggtattgaag gggcggcagg
     4201 tgaaaagacc tttgaaccag ttgcagaacg tttgaaagtg ttcggcgcat aatttcgcgg
     4261 tcaaaaaaat gccgtctgaa ccctgttcag acggcatttt tcagtatcag gcggcgagtt
     4321 ttccgcattc tgagaccttt gtttacaaat atcatgttca atatagttaa aagaaattat
     4381 tctcatttcc tccgtgaggc aatataattc ggttgttttg ttaaattgag tataaaaatg
     4441 aaaatatcat ttcatttagc tttattaccc acgctgatta ttgcttcctt ccctgttgct
     4501 gccgccgata cgcaggacaa tggtgaacat tacaccgcca cgctacctac cgtttccgtg
     4561 gtcggatagt ccgacaccag cgtactcaaa ggctacatca actacgacga agccgccgtt
     4621 acccgcaacg gacagctcat caaagaaacg ccgcaaacca tcgatacgct caatatccag
     4681 aaaaacaaaa attacggcac gaacgatttg agttccatcc tcgaaggcaa tgccggcatc
     4741 gacgctgcct acgatatgcg cggtgaaagc attttcctgc gcggttttca agccgacgca
     4801 tccgatattt accgcgacgg cgtgcgcgaa agcggacaag tgcgccgcag tactgccaac
     4861 atcgagcgcg tggaaatcct gaaaggcccg tcttccgtgc tttacggccg caccaacggc
     4921 ggcggcgtca tcaacatggt cagcaaatac gccaacttca aacaaagccg caacatcggt
     4981 gcggtttacg gttcgtgggc aaaccgcagc ctgaatatgg acattaacga agtgctgaac
     5041 aaaaacgtcg ccatccgtct taccggcgaa gtcgggcgcg ccaattcgtt ccgcagcggc
     5101 atagacagca aaaatgtcat ggtttcgccc agtattaccg tcaaactcga caacggcttg
     5161 aagtggacgg ggcaatacac ctacgacaat gtggagcgca cgcccgaccg cagtccgacc
     5221 aagtccgtgt acgaccgctt cggactgcct taccgcatgg ggttcgccca ccggaacgat
     5281 tttgtcaaag acaagctgca agtttggcgt tccgaccttg aatacgcctt caacgacaaa
     5341 tggcgtgccc aatggcagct cgcccaccgc acggcggcgc aggattttga tcatttctat
     5401 gcaggcagcg aaaacggcag cctaattaaa cgcaactacg cttggcagca gaccgacaac
     5461 aaaaccctgt cgtccaactt cacgctcaac ggcgactaca ccatcggccg ttttgaaaac
     5521 catctgaccg taggcctgga ttacagccgc gaacaccgca acccgacatt aggttacaac
     5581 cgccccttta ccgcctccat caacccctac gaccgcgcaa gctggccggc ttcgggcagg
     5641 ctgcaacccg tcttcaccca aaaccgccac aaagccgact cctacggcat tttcgtgcaa
     5701 aacatcttct ccgccacgcc cgacttgaaa ttcgtcctcg gcggtcgtta cgacaaatac
     5761 acctttaatt ccgaaaacaa actcaccggc aacagccgcc agtacagcgg ccactcgttc
     5821 agccccaaca tcggcgcagt gtggaacatc aaccccgttc acacacttta cgcctcgtat
     5881 aacaaaggtt tcgcgcctta tggcggacgc ggcggctatt tgagcatcga tacgtcatct
     5941 tctgccgtgt ttaacgccga ccccgagtac acccgccaat acgaaaccgg cgtcaaaagc
     6001 agttggctgg acaatcgttt ggacaccaca ttgtccgcct accaaatcga acgcttcaat
     6061 atccgctacc gccccgacgc ggaaaataat ccctacactt gggcagtcgg cggcaaacac
     6121 cgttcgcgtg gcgtggaatt gtccgccatc gggcaaatca tccccaaaaa actctatctg
     6181 cgcggttcgt tgggcgtgat gcaggcgaaa gtcgttgaag acaaagaaaa tcccgaccga
     6241 gtgggcatcc atttgaataa caccagcaac gttaccggca acctgttttt ccgttatacc
     6301 ccgaccgaaa acctctacgg cgaaatcggc gtaaccggta caggcaaacg ctacggttac
     6361 aactcaagaa ataaagaagt gactacgctt ccaggctttg cccgagttga tgccatgctt
     6421 ggctggaacc ataaaaatgt taacattacc tttgccgcag ccaatctgct caatcaaaaa
     6481 tattggcgtt cggatgccat gcccggcgcg ccgcgcactt atacggcgcg ggttaattac
     6541 agtttctaat cccgttcgtg caacagaatg ccgtctgaaa atcctgataa tccgtaaatt
     6601 cagcacacga actttttgac aggctttatc tacatcagta taatccaaaa agaaatagcg
     6661 cccttggcag tacgcattac ccattaaatc atagcgggta tgccgatccc aagggctttt
     6721 ttatatcttt gacaaatata ggggaagcaa tatgaaaacc gccattttga ttgacggtgc
     6781 attttttatt aagcgggtac ggtcattagg cctgtctcaa aatccacacg atgcaaaaag
     6841 aattgctgat ttggctttcc gatattcgat gctgcatctg aaagataaaa tcaacggcac
     6901 agatcaaatg gatgatttat atagaatttt cttttacgac tgcgtgccgt ttgaaaagaa
     6961 aatgcacaat cccgtatctc atcgtgccat agatttttca aagactccgg aagccatatt
     7021 tcgtcgcaat ctgcataccg aattgaagaa gaagcgtaaa ttagcgttac gtttaggcaa
     7081 gctgtcggac aatacagcat ggatattaaa accccaagtc atgaaaaatc ttctgaaaaa
     7141 cccgtcaact caaattacgg aaaacgatgt cgtgctcgat gttaaacaaa aaggtgtaga
     7201 tatgcgtata ggcttggata tttcatctat taccttaaaa aaacaagccg ataaaatcat
     7261 cttgttttct ggtgattccg attttgtccc agcagccaaa ttagccagac gggaaggtat
     7321 cgattttatt cttgatccta tgtggaatca tgtcccacct gacttaatgg aacacatcga
     7381 cggattgaag acaaaatgtc cgaaaccgta aattactaga acaaaggccg tctgaaaata
     7441 aggctaatta tctaagccat atttcagacg gcctttataa aggctgaaat aaaactaaac
     7501 acattttttc actcaaatcg aacacgcctt caataaaatg ccataaaatc cgcacattaa
     7561 tctgacacac aagagatacc tatgaaactg aaaaccttag ctttgacttc attgaccctg
     7621 ttggcattgg ccgcttgtag caaacaggct gaaaccagcg ttccggcaga cagcgtccaa
     7681 agcagctcat ctgctccggc agccccagcc ccattgaccg aaggcgtgaa ctacactgta
     7741 ttgtccacgc ctatcccgca acagcaggcc ggcaaagtcg aagtcttgga atttttcggc
     7801 tacttctgcc cgcattgcgc ccatcttgag ccggtcttga gcgagcacat caaaacgttt
     7861 aaagacgata cctatatgcg ccgggagcat gtcgtgtggg gtgatgaaat gaaacctttg
     7921 gcacgtttgg cggccgcagt ggaaatggcc ggtgaatcag ataaagccaa cagccatatt
     7981 ttcgatgcga tggttaatca aaaaatcaat ctggccgata ccgataccct gaaaaaatgg
     8041 ctgtccgagc aaacagcgtt tgacggcaaa aaagtattgg ctgcatttga ggcttctgaa
     8101 agccaagcgc gtgcggctca aatggaagag ttgaccaata aattccaaat cagcggcaca
     8161 ccgactgtga tcgtcggcgg caaataccaa gttgaattta aagactggca gtccggtatg
     8221 accacgattg accagttggt ggataaagta cgcgaagagc agaaaaagcc gcaataagtt
     8281 gaggattgaa tgagtaaagg ccgtttgaaa atag
//
LOCUS       DAWHSK010000066         8215 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.066, whole genome shotgun sequence.
ACCESSION   DAWHSK010000066 DAWHSK010000000
VERSION     DAWHSK010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8215
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.066"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(264..2954)
                     /gene="glnE"
                     /locus_tag="WIC03_000946"
     CDS             complement(264..2954)
                     /gene="glnE"
                     /locus_tag="WIC03_000946"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HEZ3883469.1"
                     /translation="MSDNRLDTARRHSLFLARQLDNGKLKPEIFLPMLDKVLTEADFQ
                     AFADWDKIRAEENEEELARQLRGLRRYVVSQIIVRDINRISDLNEVTRTITLFADFAV
                     NTALDFAYAYYRDMYGTPIGRYTKSPQHLSVVAMGKAGGYELNVSSDIDLIFVYPESG
                     DTDGRRERGNQEFFTKVGQKLIALLNDITADGQVFRVDMRLRPDGDSGALVLSETALE
                     QYLITQGREWERYAWCKGRVVTPYPNDIKALVRPFVFRKYLDYGAYEAMRKLHRQIRS
                     EVSKKGMADNIKLGAGGIREVEFIAQIFQMIRGGQMRALQLKGTQETLKKLAETGIMP
                     SENVETLLAAYRFLRDVEHRLQYWDDQQTQTLPTSPEQRQLLAESMGFDSYSAFSDGL
                     NVHRNKVNQLFNEILSEPEEQTQDNSEWQWAWQDKPDEEGRRCRLKAHGFDAETVAAR
                     LDQIRHGHKYRHLSAHAQPRFDAVVPLFVQAAAAQSNPTDTLMRLLDFLENISRRSAY
                     LAFLNEHPQTLAQLAQIMGQSSWVAAYLNKYPILLDELISAQLLDTAFDWQALAAALS
                     DDLKACGGDTEAQMDTLRRFQHAQVFRLAVQDLAGLWTVESLSDQLSALADTILAAAL
                     LCAWADMPKKHRDTPQFAVVGYGKLGGKELGYASDLDLVYLYDDPHPDAGDVYSRLAR
                     RLTNWLSAATGAGSLYETDLRLRPNGDAGFLAHSIAAFEKYQRENAWTWEHQSLTRAR
                     FICGTSEIQTAFDRIRTEILTAERDQTALAGEIIEMREKMFPTHPPADSNVKYARGGV
                     VDVEFIVQYLILAHARQYPQLLDNYGNIALLNISADCGLIDKTLAGQSRTAYRFYRRQ
                     QHNTKLRDAAKTEVTGELLAHYGNVRKLWREVFGEEAATA"
     gene            complement(3134..3469)
                     /locus_tag="WIC03_000947"
     CDS             complement(3134..3469)
                     /locus_tag="WIC03_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883470.1"
                     /translation="MTVDKEQVEKMIYSENKQSVIDGMLGMTFSSEEDEIPWISEKLT
                     ELSKHKDLDIARLSLTCFGHLARMHENIGDCDKVIALLLSKQGDPDFQGFAEDALDEI
                     SLFIFKKRP"
     gene            complement(3723..4196)
                     /locus_tag="WIC03_000948"
     CDS             complement(3723..4196)
                     /locus_tag="WIC03_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2247 family protein"
                     /protein_id="HEZ3883471.1"
                     /translation="MNLDLTAQKVRLSWKDILWGYGNKYLGWADVAAYARKMTLSDHD
                     ERVFKLSLINKSNILELKPVLEDLASEMRDYSPKNWLYVLLSDVFHRKEEFEDPLGEV
                     EKIYADFDYPEEIESFVRYMPPKDGYIPSAHTYEENIARLYSHWEHYLNNGGGQG"
     gene            complement(4249..4530)
                     /locus_tag="WIC03_000949"
     CDS             complement(4249..4530)
                     /locus_tag="WIC03_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883472.1"
                     /translation="MENGQRNNRFPLEKRIFYLEHSGRYLMICALSDYSQNKHTVVMA
                     NFIYPDEKTDWRNLDDLFNELVLEELQASFMDWHPTVEEAISRHLEDFS"
     gene            complement(4617..4940)
                     /locus_tag="WIC03_000950"
     CDS             complement(4617..4940)
                     /locus_tag="WIC03_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883473.1"
                     /translation="MFKDELNEFIRLISDPESELDEWYLSDFKDEHIWEMQSYEAFSC
                     LREAVPYLFAYPRYGYELLEIISALKETSDTTELFYEPGIVPLLIDLYKEDSYLVNMV
                     KRIFK"
     gene            complement(5078..5257)
                     /locus_tag="WIC03_000951"
     CDS             complement(5078..5257)
                     /locus_tag="WIC03_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883474.1"
                     /translation="MPSERTFCRSDGIFISFFRLPPLENPLRAALFETKQTPAANRTI
                     LIRSQSKSGGKLLNI"
     gene            complement(5273..6280)
                     /locus_tag="WIC03_000952"
     CDS             complement(5273..6280)
                     /locus_tag="WIC03_000952"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEZ3883475.1"
                     /translation="MYPLARRILFALDAEKAHHFTLDALYTVYKLGLIPVTGNRTKPV
                     KLMGMDLPNPVGLAAGLDKNGEYIDALGALGFGFIEIGTVTPKPQPGNPQPRLFRVPE
                     HQGIINRMGFNNHGIDTMIRNIEKSKFSGVLGINIGKNAVTPIENAADDYLICLEKAY
                     AHASYITVNISSPNTKNLRALQGGDELSALLEALKNKQAQLASVYGKYVPLAVKIAPD
                     LDEAQIEDIAHVVKSVEMDGIIATNTTIDKSSLGSHPLAGEQGGLSGRPVHEKSNRVL
                     KLLADHIDGKLPIIGVGGIMEGRDAADKIRLGATAVQVYSGLIYKGPALVKECLKALA
                     R"
     gene            6512..6748
                     /gene="acpP"
                     /locus_tag="WIC03_000953"
     CDS             6512..6748
                     /gene="acpP"
                     /locus_tag="WIC03_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEZ3883476.1"
                     /translation="MSNIEQQVKKIVAEQLGVNEADVKNESSFQDDLGADSLDTVELV
                     MALEEAFGCEIPDEDAEKITTVQLAIDYINAHNG"
     gene            6904..8151
                     /gene="fabF"
                     /locus_tag="WIC03_000954"
     CDS             6904..8151
                     /gene="fabF"
                     /locus_tag="WIC03_000954"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEZ3883477.1"
                     /translation="MSQRRVVITGLGQVSPVGNTVAEAWDTLLTGKSGIGAITRFDAS
                     DINSRVAGEVRGFDIGQYISAKEARRMDVFIHYGIAAALQAIADSGLDDVENLDKDRI
                     GVNIGSGIGGLPGIEVTGKAVIEGGARKINPFFIPGSLINLISGHVTILKGYRGPSYG
                     MVSACTTGAHAIGDSLRMIKYGDADIMVAGGAEGAISTLGVGGFAAMKALSTRNDDPA
                     TASRPWDKGRDGFVIGEGAGILVLEELEHAKKRGAKIYAEIVGFGMSSDAYHITAPNE
                     EGPALAVTRALKDAGINPEDVDYVNAHGTSTPLGDANETKALKRAFGEHAYKTVVSST
                     KSMTGHLLGAAGGVEAVYSILAIHDGKIPPTINIFEQDVEAGCDLDYCANEARDAEID
                     VAISNSFGFGGTNGTLVFKRFKG"
BASE COUNT         1900 a         2161 c         2116 g         2038 t
ORIGIN      
        1 gtagaatgcg ctattttaat ggaaacggcg gggttttaaa cggttcttat ttgtttgttt
       61 tgatttttgc ttgggtaaaa aatcccatca ttcccacaaa aacagaagcc tgaaatcccg
      121 tcattcccgc gaaagaggaa tccggttttt gggtttcagc cattcccgat aaatcgcttt
      181 agctctgccg caaccttaaa acggcacggt acgaaaatac cgtctgaaac ccagattgtc
      241 aggcttcaga cggcattttt tgttcaggcg gttgccgctt cttcgccgaa cacttcccgc
      301 cacaatttcc tgacattgcc gtaatgtgcc aacagttcgc cggttacttc ggtttttgcc
      361 gcgtcgcgca gtttggtgtt gtgctgctgc cggcggtaga agcgataggc ggtgcggctt
      421 tgtccggcga gggttttgtc aatcaaaccg cagtcggcgg agatgtttaa gagggcgatg
      481 ttgccgtagt tgtccaagag ttgcggatac tggcgggcat gggcaagtat cagatattgg
      541 acgataaatt cgacatcgac cacgccaccg cgcgcgtatt tgacgttgct gtcggcaggc
      601 gggtgggtgg ggaacatttt ttcgcgcatt tcgatgattt cgcctgccaa ggcggtttgg
      661 tcgcgttcgg cggtgaggat ttcggtgcgg atgcggtcga aggccgtctg aatttcggac
      721 gtgccgcaga tgaagcgggc gcgggtaagg gattggtgtt cccacgtcca ggcgttttcg
      781 cgctggtatt tttcaaaggc ggcgatgctg tgggcgagga aaccggcgtc gccattaggg
      841 cgcaggcgca ggtcggtttc gtagaggctg cctgcgccag tggcggcgga aagccagttg
      901 gtcaggcgac gggcgaggcg gctgtacacg tcgcctgcgt cggggtgggg atcgtcgtag
      961 aggtagacga ggtcgaggtc ggaggcgtag ccgagttctt taccgccgag tttgccgtag
     1021 ccgacgacgg cgaattgcgg tgtgtcgcgg tgttttttgg gcatgtccgc ccatgcgcac
     1081 agcagggcgg cggcgaggat ggtgtcggcg agggcggaga gttggtcgga gagggattct
     1141 accgtccaca gtccggcgag gtcttggacg gcgagacgga agacttgggc gtgctggaag
     1201 cggcgcaggg tgtccatttg cgcttcagta tcgccgccgc aggctttgag gtcgtctgaa
     1261 agggcggcgg cgagcgcctg ccaatcaaac gcggtatcca aaagctgcgc gctgatgagt
     1321 tcgtccaaca aaatcggata tttgttcaga tacgccgcca cccaagaact ttggcccata
     1381 atctgcgcca gttgcgccaa ggtttgcgga tgttcgttga ggaaggcgag ataggcggat
     1441 cggcggctga tgttttcgag aaaatccaac agccgcatca atgtatcggt cgggttgctt
     1501 tgcgctgccg ccgcctgtac gaacagcggc acaaccgcat cgaaacgcgg ctgggcgtgt
     1561 gcggaaagat ggcggtattt atggccgtgg cggatttggt cgagccttgc ggcgacggtt
     1621 tcggcatcga acccgtgcgc cttcagacgg catcgccgcc cttcttcgtc gggtttgtcc
     1681 tgccatgccc attgccattc gctgttgtct tgcgtttgct cttcgggttc gctcaaaatt
     1741 tcgttgaaca actgattgac tttgttccga tgaacattga gaccgtctga aaaagcggaa
     1801 taactgtcga aacccatgct ttcagcgagc agttgccgct gttcaggcga agtgggcagg
     1861 gtttgggttt gttggtcgtc ccagtattgc aggcggtgtt cgacgtcgcg caggaagcgg
     1921 taggcggcaa gcagggtttc gacgttttca gacggcatga tcccggtttc ggcaagtttc
     1981 ttcagcgttt cctgcgtgcc tttcagttgc agcgcacgca tttggccgcc gcgtatcatc
     2041 tggaaaatct gggcgataaa ttcgacttcg cggatgccgc ccgcgccgag tttgatgttg
     2101 tccgccatgc cttttttgct gacttcgctg cggatttggc ggtgcagctt acgcatcgcc
     2161 tcatacgcgc cgtaatccag atatttgcgg aacacaaagg ggcgcaccag tgctttgatg
     2221 tcgttcggat acggcgtaac cacgcgacct ttgcaccacg cgtagcgttc ccattctcgc
     2281 ccctgtgtaa tcaaatattg ctccagcgcg gtttcgctca ataccaacgc gcccgaatca
     2341 ccgtccggcc gcagccgcat atcgacgcgg aacacttgcc cgtcggcggt aatgtcgttc
     2401 agcaacgcaa tcagtttctg cccgactttg gtgaaaaact cctgattgcc gcgttcgcgc
     2461 ctgccgtcgg tgtcgcccga ttcgggatag acgaaaatca aatcgatgtc ggaagacacg
     2521 ttcaactcat agccgcccgc cttgcccatc gccaccacgc tcaaatgctg cggcgatttg
     2581 gtataacgcc cgatcggcgt gccgtacatg tcccgataat aggcgtaggc aaaatccagc
     2641 gcggtattga cggcaaaatc ggcaaacagc gtaatcgtgc gggttacttc gttcaaatcg
     2701 ctgatgcggt ttatatcgcg cacgataatc tgcgacacca cataacggcg caacccgcgc
     2761 aactgccgcg ccaattcttc ctcgttttct tccgcacgga ttttgtccca gtcggcaaag
     2821 gcttggaaat ccgcttcggt caaaaccttg tccagcatag gcaggaaaat ttcgggcttg
     2881 agtttgccgt tgtcgagctg gcgggcgagg aagagggaat ggcggcgggc ggtgtcgagg
     2941 cggttgtcgg acatttcgga ttccgtttga aaaaatggca ggaagatgat ggcggattca
     3001 cttcaaatca ggacagggcg aggggatgcc gtaccggttt aaagtcaagc cactatatca
     3061 tagcaacctt cgatgccgtc tgaaagccgg ataacgcttc agacggcatc ggattgacag
     3121 gtttaatccg cgcttacgga cgctttttaa atataaataa agaaatctcg tctaacgcat
     3181 cttcggcaaa accttgaaaa tccggatcac cttgttttga gagcaataaa gcaatcactt
     3241 tatcgcaatc gccgatattt tcatgcatcc tggcgagatg tccgaaacag gttaatgaca
     3301 atctggcaat atccaagtct ttatgtttcg acaactccgt caacttctcg gaaatccagg
     3361 ggatttcatc ctcctccgag ctgaaggtca taccgagcat accgtcaata acactctgtt
     3421 tattttcaga ataaatcatc ttttctactt gttccttatc aaccgtcatt ttttaatttt
     3481 tccttttaaa ttaaccaatc ctgctttctt taaagcattt ttgccgggcg gcaggattgt
     3541 tatcaaacat ttgcacccgg tccggcaaaa cgatttcctt atcggtttat cggcaacgat
     3601 gcgggcggtt cgggttttgc cgcttccgga atgcagcccg cccccgcttt caaatcttcc
     3661 gctctaatcc gtttgccgtc tgaaggccgg ataatgcttc agacggcatc ggattgccgg
     3721 ttttaaccct gccctccgcc gttgttcaaa tagtgttccc agtgagaata taaccgggca
     3781 atattttctt cataggtgtg ggcagaagga atataaccgt ctttgggcgg catatacctg
     3841 acaaatgatt ctatttcttc cggataatca aaatctgcat aaattttttc aacttccccc
     3901 aaaggatcct caaattcttc ttttctatgg aatacatcgc ttaagaggac gtacagccaa
     3961 tttttagggg aataatccct catttccgaa gccaaatctt ccagaacagg ctttaattca
     4021 agaatattgg atttgttgat taaagatagt ttgaacacac gttcatcatg atctgaaagc
     4081 gtcatttttc gggcataagc tgccacatca gcccaaccca agtatttatt cccatacccc
     4141 cacagaatat ccttccaaga aagacggact ttttgcgcgg ttaaatcaag gttcattttt
     4201 tactccgatt gcctgccccg tcattccgcc ccttccgatt gacgcctgtt acgaaaagtc
     4261 ttccaaatga cggctgattg cctcttcaac agtcggatgc caatccataa atgaagcctg
     4321 caattcctct aaaaccaact cattgaataa atcatccaaa ttccgccaat ccgttttttc
     4381 atccggatag atgaaatttg ccataacgac agtatgtttg ttttgggaat aatccgataa
     4441 ggcgcaaatc atcagatacc gccccgaatg ctccagataa aaaattcgtt tttccaaagg
     4501 aaaccggtta ttccgttgcc cgttttccat ttttcatcct ttttaacgta tcggcttccg
     4561 cgtccttcaa attgagttac ctgccttccg ccaagcaaac gcccgcgccc ctttcatcat
     4621 ttgaatatcc gtttgaccat atttaccaga taactatctt ctttatataa atcaatcagc
     4681 aaaggcacga tacccggctc ataaaataat tctgtcgtat cagatgtttc ttttaacgcg
     4741 gaaattatct ccaacagttc atacccgtat cggggataag caaacaaata ggggactgct
     4801 tcccgcaaac atgaaaatgc ttcgtaactt tgcatttccc atatatgttc atctttaaaa
     4861 tcactcaaat accattcatc caattcactt tccggatcgg atatcaatcg gataaattca
     4921 tttagttcgt ccttaaacat atcaaatcct taaatataaa atcttttgat tatccgccgt
     4981 atccttattt tccacatcat gaatttcaac gccgttcata ttttgcgttt tctcaaaccg
     5041 tccaccacgg cataaaatta ttttttgtat caattgctta tatatttaac aacttaccgc
     5101 cggacttgct ttggctgcgt atcaagattg tccggttcgc ggcgggcgtt tgctttgttt
     5161 caaacagggc ggcgcgtaag ggattttcaa ggggcggcag gcggaaaaag gaaatgaaaa
     5221 tgccgtctga acggcaaaac gtgcgttcag acggcatttt gggcggatcg cgtcatcgcg
     5281 ccaaagcctt caaacattct ttgaccaatg ccggaccttt gtatatcaat ccgctgtaca
     5341 cctggacggc agttgcgccc aagcggattt tatctgccgc gtccctgcct tccataatgc
     5401 cgcctacgcc gataatcggc agcttgccgt ctatgtggtc tgccaacagc ttcaacaccc
     5461 gattactttt ttcatgaacg ggccgcccgc tcaaaccgcc ctgctcgcct gcgagcggat
     5521 ggctgccgag acttgatttg tcgatggtgg tattggtagc gatgatgccg tccatttcga
     5581 cggatttgac aacgtgggcg atgtcttcga tttgtgcttc atccaaatcg ggggcgattt
     5641 tgacggcgag cggaacgtat ttcccgtata cagaggcaag ctgtgcctgt ttgtttttca
     5701 aagcctcaag caatgcgctc aactcgtcgc caccttgcag cgcgcggagg tttttggtgt
     5761 tgggcgagga aatgttgacg gtaatgtaac ttgcgtgtgc gtaggctttt tcaaggcaga
     5821 ttaaataatc atcggcagcg ttttcgatgg gtgtaaccgc gtttttaccg atgttgatgc
     5881 ccaatacgcc actgaattta cttttttcga tgttgcgtat catggtgtcg ataccgtggt
     5941 tgttgaaacc catgcggttg atgatgcctt ggtgttcggg aacgcggaag aggcgcggct
     6001 gcgggttgcc gggctgcggt ttgggcgtta ccgtgccgat ttcgatgaaa ccaaagccga
     6061 gcgcgcccaa tgcgtcgatg tattcgccgt ttttgtcgag tccagcggca agtccgacag
     6121 ggttgggcaa atccataccc atcaatttta caggtttggt acggttgccg gttacgggaa
     6181 tcagacccaa tttataaacc gtgtagagcg cgtcgagcgt gaagtggtgg gctttctcgg
     6241 catcgagtgc aaacaggatg cggcgggcaa gtggatacat gacagggctt tctgcttcag
     6301 atggaaatgc cgtcatttta accgaaatcc cgtcaaacat acggcacaca ggtcaaactt
     6361 gaacgacaaa acccgccgca cccaaaaatg tacaaatcgg caaatattga tataatagtc
     6421 attagataga aaactctatt aaaagtcggt acaataccac cgatttattt cccttaaaca
     6481 acctgccgca aggcataaag gaacgactga tatgtcaaac atcgaacaac aagttaaaaa
     6541 aattgttgct gaacaactgg gcgtaaacga agccgacgtg aaaaacgaat cttctttcca
     6601 agacgacttg ggcgcggatt ctttggacac cgtggagctg gttatggctt tggaagaagc
     6661 attcggctgc gaaatccccg acgaagatgc cgaaaaaatc accaccgtcc aactggctat
     6721 cgactacatc aatgcccaca acggctaacc ggtcgtcgcc cgacacaaca gcctctgctg
     6781 caccgcgcaa tagaggcttt tcccttatat ggcaaactgt ttgaacccct gccgcaaatt
     6841 acagacggca tctgcacgac agccgcatat gcccccattc cgacaaccaa cagcgaggtt
     6901 atcatgagtc agagaagagt agtcattaca ggcttaggtc aggtttcccc tgtcggcaac
     6961 actgtcgcag aggcttggga caccctgctc accggcaaaa gcggcatcgg cgcgattacc
     7021 cgctttgacg catccgacat caacagccgt gtcgccggcg aggtgcgcgg tttcgacatc
     7081 ggacaataca tcagcgcgaa agaagcgcgc cggatggacg tattcatcca ctacggcatt
     7141 gccgccgcat tgcaggcaat cgccgattcg ggtttggacg atgtggaaaa cctcgacaaa
     7201 gaccgcatcg gcgtgaacat cggttccggc atcggcggac tgcccggcat cgaggtcacc
     7261 ggcaaagccg taatcgaagg cggcgcgcgc aaaatcaacc ctttctttat ccccggttct
     7321 ctgattaatc tgatttccgg acacgttacc atcctcaaag gctaccgcgg cccgagctac
     7381 gggatggttt ccgcctgcac caccggcgcg cacgccatcg gcgattccct ccgtatgatt
     7441 aaatacggcg acgcggacat aatggttgcc ggcggcgcgg aaggcgcaat cagcactttg
     7501 ggcgtgggcg gttttgccgc gatgaaagcc ctctccaccc gcaacgacga ccccgccacc
     7561 gcttcccgtc cgtgggacaa aggccgcgac ggcttcgtta tcggcgaagg cgcgggcata
     7621 ttggtgttgg aagaattgga acacgccaaa aaacgcggcg cgaaaatcta cgccgaaatc
     7681 gtcggcttcg gcatgagttc cgatgcttac catatcaccg cgccgaacga agaaggcccc
     7741 gcccttgccg ttacccgcgc gctgaaagat gccggcatca atcccgaaga cgtggattac
     7801 gtcaacgcgc acggcacgtc cacccccttg ggcgatgcca acgaaaccaa agccctcaaa
     7861 cgcgcgttcg gcgaacacgc ctacaaaacc gtcgtcagct cgaccaaatc catgaccggc
     7921 cacctgctcg gcgcggcggg cggcgtggag gccgtgtaca gcattttggc gatacacgac
     7981 ggcaaaatcc cgccgaccat caacattttt gaacaagacg ttgaagccgg ctgcgatttg
     8041 gactactgcg ccaacgaagc gcgcgacgcg gaaatcgacg ttgccatttc caactccttc
     8101 ggcttcggcg gcaccaacgg cacgctggtc ttcaaacgct tcaaaggctg attccgcaaa
     8161 gccgccgccg acatcgaaat gccgtctgaa accgtttcag acggcatttt tatag
//
LOCUS       DAWHSK010000067         8151 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.067, whole genome shotgun sequence.
ACCESSION   DAWHSK010000067 DAWHSK010000000
VERSION     DAWHSK010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8151
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.067"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..139)
                     /locus_tag="WIC03_000955"
     CDS             complement(<1..139)
                     /locus_tag="WIC03_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEZ3883478.1"
                     /translation="MSVQTVTYNKFFMNTNQPAVYDPLTRALHWLTVAGFIGILTTIV
                     LW"
     gene            381..1535
                     /gene="obgE"
                     /locus_tag="WIC03_000956"
     CDS             381..1535
                     /gene="obgE"
                     /locus_tag="WIC03_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEZ3883479.1"
                     /translation="MKFIDEAKIEVAAGKGGNGATSFRREKFVPRGGPDGGDGGKGGS
                     VWAEADENTNTLVEYRFVKRYQAKNGEKGHGSDRYGAGADDIVLKMPVGTLIRDLDTG
                     ETVADLTYHGQRVCLAKGGKGGLGNIHFKSSVNRAPKQSTPGEEGEARSLQLELKVLA
                     DVGLLGMPNAGKSTLITAVSAARPKIANYPFTTLHPNLGVVRIDENHSFVMADIPGLI
                     EGAAEGAGLGHRFLKHLSRTGLLLHVVDLAPFDETVNPAEEALAIINELRKYDEELYG
                     KPRWLVLNKLDMLDEEEARARTAAFLEAVGWDYPKPDDRFQFDMETPRLFQISALTHQ
                     GTQELVHQINQYLIEKKRIEAEKAEAERAAANVEIAEQQPKTDTGVFKPE"
     gene            1618..2667
                     /locus_tag="WIC03_000957"
     CDS             1618..2667
                     /locus_tag="WIC03_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEZ3883480.1"
                     /translation="MSSNQYIQSLELTNFTVLPNDTFEFSENLNVIVAENGCGKTHLL
                     KILYSLLEVTSNTKNRLLKTELQKSFADKLLNVFRPDSLGRLSKRLQGRGRTEIVLKL
                     QNGTTHSSLNFSSNSSSQVNVQSIGLKENEYTPTPIFLPSRELITLCPWFTSLYQNQS
                     IPFEETWFDTCMQLNHPLAKGPRETKIRELLEPIENAMGGKVSEEQGRFYLSLTNTGG
                     KIEAPLVAEGLRKFVMIARLIATGALLDKGYLFWDEPEANLNPKLIKVAARIIWSLSQ
                     QGIQVFIATHSLFLLRELELLKLENNTDLPVRFFSLIASDDGTKVEQGNSINDLNTLI
                     LLDENIMQADRYLNI"
     gene            3355..4776
                     /locus_tag="WIC03_000958"
     CDS             3355..4776
                     /locus_tag="WIC03_000958"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEZ3883481.1"
                     /translation="MTTIYNTLTRQKEPFAPIDPENVRMYVCGMTVYDYCHLGHARVM
                     VVFDMIARWLRECGYPLTYVRNITDIDDKIIARAAENGETIGKLTARFIQAMHEDADA
                     LGVLRPDIEPKATENIPQMIAMIETLIQNGKAYPAANGDVYYAVREFAAYGQLSGKSL
                     DDLRAGERVEVDGFKRDPLDFVLWKAAKAGEPAWESPWGRGRPGWHIECSAMSENLFG
                     DTFDIHGGGADLQFPHHENEIAQSVGASGHTCGHDHAQTHHGQSIASHVKYWLHNGFI
                     RVDGEKMSKSLGNFFTIREVLKQYDPEVVRFFILRAHYRSPLNYSDAHLDDAKGALTR
                     LYTTLKNTPAAAFDLSENANDYTRRFYAAMNDDFGTVEAVAVLFELAGEVNKTNDVHL
                     AGCLKALGGIIGLLQRDPIEFLQGGAVSDGLSNEEIDDLIARRKQARADKNWAESDHI
                     RDLLNEHKIILEDNAGGTTWRRG"
     gene            5197..5976
                     /gene="xth"
                     /locus_tag="WIC03_000959"
     CDS             5197..5976
                     /gene="xth"
                     /locus_tag="WIC03_000959"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEZ3883482.1"
                     /translation="MLKIISANVNGIRSAYKKGFYEYIAASGADIVCVQELKAQEADL
                     SADMKNPHGMHGYWHCAEKRGYSGVAVYSKRKPDNVQIGMDIEEFDREGRFVRCDFGR
                     LSVISLYLPSGSSAEERQQVKYRFLDAFYPMLEAMKNEGRDIVVCGDWNIAHQNIDLK
                     NWKGNQKNSGFLPEEREWIGKVIHKLGWTDMWRTLYPDVPGYTWWSNRGQAYAKDVGW
                     RIDYQMVTPELAAKAVSAHVYKDEKFSDHAPLVVEYDYAAE"
     gene            5963..6307
                     /locus_tag="WIC03_000960"
     CDS             5963..6307
                     /locus_tag="WIC03_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883483.1"
                     /translation="MLPNKVLGKYDWNVDGKTGIGAAWVVAAFILPILVWAIFMLSRM
                     QGWLAPTKANPIWALVWLLICMPCLLIASKCLGWKGWRLLANVLACLAVCALLSVPAA
                     LLIAFTLQDLLK"
     gene            6317..6847
                     /locus_tag="WIC03_000961"
     CDS             6317..6847
                     /locus_tag="WIC03_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEZ3883484.1"
                     /translation="MESFELEDLTLWLVRDADEAEMWIDRWAISYPVVQMSEASAGQS
                     IGEWQAGLQTAFERIRGKYVAVVAHGAGAAAFLAWLYQADILTRKKIANIILVPQRPD
                     IFSDDAEHTFQRVRCPCRAALVVPEHGGVPHGWAKKQADLWNARLLVSPHSGSLNGML
                     GGWQWGMKLMQEMLLA"
     gene            complement(7075..>8151)
                     /gene="asd"
                     /locus_tag="WIC03_000962"
     CDS             complement(7075..>8151)
                     /gene="asd"
                     /locus_tag="WIC03_000962"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEZ3883485.1"
                     /translation="SVLMQRMKEENDFAHIPEAFFFTTSNVGGAAPDFGQAAKTLLDA
                     NNVAELAKMDIIVTCQGGDYTKSVFQALRDSGWNGYWVDAASSLRMKDDAIIVLDPVN
                     RNVIDNGLKNGVKNYIGGNCTVSLMLMALGGLFQNDLVEWATSMTYQAASGAGAKNMR
                     ELISGMGAVHAQVADELADPAGSILDIDRKVSDFLRSEDYPKANFGVPLAGSLIPWID
                     VDLGNGQSKEEWKGGVETNKILGRSDNPTVIDGLCVRVGAMRCHSQAITLKLKKDLPV
                     SEIETILAGANDWVKVIPNEKEASIHELTPAKVTGTLSVPVGRIRKLGMGGEYISAFT
                     VGDQLLWGAAEPLRRVLRIVLGSL"
BASE COUNT         2174 a         1987 c         2095 g         1895 t
ORIGIN      
        1 tccacaggac aatggtggtc agaatgccga tgaagccggc aacggtcagc cagtgcagcg
       61 cacgtgtcaa cgggtcgtaa acggcaggtt ggtttgtgtt cataaaaaac ttattatatg
      121 taacggtttg aacggacatt ccgtcgggtc ggaatgtcaa aaaggcgcga ttgtaccaaa
      181 gaagttggac ataattttgt ttgcaggctg aagatttgct taaaaattca ttaagatggg
      241 cggaacaaat agtttgggta cggcgtgttg aaataccgct tatcccttat aatagcgtcc
      301 gaaattctgt tcggacggca tcaaaacaca cggtaatccg tctgaacccc ccatttgaaa
      361 ccaacaaaca agagcattga atgaaattca tcgacgaagc aaaaatcgaa gtcgccgcag
      421 gcaaaggcgg taatggcgca accagtttcc gccgcgaaaa attcgtaccg cgcggcggcc
      481 cggacggcgg cgacggcggc aaaggcggca gcgtctgggc agaagccgac gaaaacacca
      541 acaccctcgt cgaataccgc ttcgttaaac gctaccaagc caaaaacggc gaaaaaggcc
      601 acggttccga ccgttacggc gcaggtgcgg acgacatcgt cctcaaaatg cccgtcggca
      661 cccttatccg cgacctcgac accggcgaaa ccgttgccga cctcacttat cacggacagc
      721 gcgtctgcct cgccaaaggc ggcaaaggcg gcttgggcaa catccacttc aaatcgtccg
      781 tcaaccgcgc cccgaaacaa tccacccccg gcgaagaagg cgaagcccgt tccctgcaac
      841 tcgaactcaa agtcctcgcc gatgtcggct tattgggtat gcccaacgcc ggcaaatcca
      901 ccctgattac cgccgtatcc gccgcacgcc ccaaaatcgc caactacccc ttcaccaccc
      961 tgcatccaaa cttaggcgtg gtacgcatcg acgaaaacca cagcttcgtg atggccgaca
     1021 tccccggcct gattgaaggc gcggcagaag gcgcaggcct cggccatcgt ttcctcaaac
     1081 acttatcacg taccggcctg ctgctgcacg tcgtcgattt ggccccgttt gacgaaaccg
     1141 tcaaccccgc cgaagaagcc ctcgccatca tcaacgaatt gcgcaaatac gacgaagaac
     1201 tctacggcaa accacgctgg ctggtgctga acaaactcga catgctcgat gaagaagaag
     1261 cccgggcgcg aacagccgcc ttcctcgaag ccgtcggctg ggactacccg aaaccagacg
     1321 accgcttcca attcgacatg gaaaccccgc gcctcttcca aatcagcgcg ctgacccacc
     1381 aaggcacgca ggaattggta caccaaatca accaatacct gatcgagaaa aaacgtatcg
     1441 aagctgaaaa agcggaggcg gagcgggcag cggcaaatgt tgagattgcc gagcaacagc
     1501 ctaaaacgga tacgggcgtg tttaagccgg agtgaaaaaa gaaagggttt cagacgatat
     1561 ttgttctaca aaaggttgtt tgaaacaatt ttaaagcaag attgaaatgg attaaaaatg
     1621 tcatctaacc aatatatcca gtcgcttgag ttgacaaact ttaccgttct tccaaacgat
     1681 acttttgaat tttctgaaaa tctcaatgtt attgttgccg aaaacggttg tggcaaaaca
     1741 catttgttaa aaattttata cagcctgcta gaagtaacat ctaacaccaa aaacagatta
     1801 ctgaaaacag aattgcaaaa aagttttgcc gataaattac tgaatgtttt ccgtcccgat
     1861 tccttgggca ggttgagcaa acgtttgcag gggcgcgggc gtacggaaat cgttttaaaa
     1921 ctgcaaaacg gcactacgca tagtagtctg aatttttcca gtaactcatc atcgcaagtc
     1981 aatgttcaaa gcatcggatt gaaagaaaat gaatatactc cgacacctat cttcttaccc
     2041 agccgcgaat taattacact ttgcccttgg ttcacttctt tgtatcaaaa ccaaagcatc
     2101 cctttcgaag aaacttggtt tgatacctgt atgcaattaa atcacccgct tgccaaaggc
     2161 cccagagaaa caaaaattcg agaattgctc gaacctattg agaacgcgat gggaggaaaa
     2221 gtatctgaag aacaaggaag gttttatcta tcgctgacaa atacgggcgg taagattgaa
     2281 gcacctttgg tagcagaggg tttgcggaaa tttgtcatga ttgcccggtt aatcgcaaca
     2341 ggggcattgc tggataaagg ctatctattt tgggatgaac cggaagctaa tctgaatcct
     2401 aaattgatca aagtggctgc ccgtattatt tggtctttat ctcaacaggg tattcaggtt
     2461 tttattgcaa cacactctct attcttattg agagaattgg aacttttgaa gcttgaaaat
     2521 aacacggatt tacctgtacg ttttttctcg ttgattgctt ccgatgatgg aacaaaagtg
     2581 gagcaaggga acagtattaa tgatttgaac acattgatat tgttagatga aaacattatg
     2641 caggcagaca ggtatctgaa tatttgaggt taagctatga ctacaatcac agaaaagaga
     2701 ttaacctttg cttttcctga agattattac gttaccaagt atgacgaatg ggaacattat
     2761 aaaatttttc agaacagttg taatttacgt aataaaattg atacaaatga aaagggaaag
     2821 aacgggataa atcaaagtgt agatgacgat aatggcagct caggtgtaga tataatagcc
     2881 cttcatgagt cgacactttg gctaatcgaa attaaagatt attaccgact tgggcttgag
     2941 ccaaatgccc aatcaataga tgaaaaactt tctgacctac catatttaat cgcaagaaaa
     3001 attcgagatt cattggcagg attggtttct gctaaattta aagcagaaaa acaggaagaa
     3061 aaagattttt ctcgcttggc tttagattgt aacgaaataa aaatagtcct tcatattgaa
     3121 atgcctagta taagaagtaa actttatccc tcgtcatcag atttagcaaa cttattaaaa
     3181 gataagttta aattatcaga atttacaaaa aattttgcaa actgttatgc agaaccaatt
     3241 ttcacaaata tcagccatat taataatccg caactacgta atgttccttg gtccgtatct
     3301 acaggaacag aacagaaatt atcatccgag caacaaagat taatccataa tcccatgacc
     3361 accatctaca acacccttac ccgccaaaaa gaaccctttg cccccatcga ccctgaaaac
     3421 gtgcgtatgt acgtttgcgg catgactgtt tacgactatt gccacttagg ccatgcccgt
     3481 gtgatggtgg tgttcgacat gattgcccgt tggttgcgcg agtgcggcta tccgctcact
     3541 tatgtacgca acatcaccga catcgacgat aaaatcattg cccgcgcggc tgaaaacggc
     3601 gagaccatcg gcaaactgac tgcgcgtttc atccaagcca tgcacgaaga tgccgatgct
     3661 ttgggcgtgt tgcgtccgga tattgagccg aaggcaacgg aaaacattcc gcaaatgatt
     3721 gccatgattg aaaccctgat tcaaaacggc aaggcatatc ctgccgcaaa tggcgacgtt
     3781 tactacgccg tgcgcgagtt tgccgcttac ggacaactgt cgggcaaatc gttggacgac
     3841 ctgcgcgcgg gcgaacgcgt ggaagtggac ggtttcaaac gcgatccgct tgattttgta
     3901 ttgtggaaag cggcgaaagc aggcgaaccg gcgtgggaaa gcccgtgggg caggggccgg
     3961 ccgggttggc acattgaatg ctctgccatg agtgaaaacc tgttcggcga taccttcgac
     4021 attcacggcg gcggcgcgga tttgcaattc ccgcatcatg aaaacgaaat cgcccaaagc
     4081 gtcggtgcca gcggtcatac ctgcggccac gatcacgcgc aaacccacca cggtcaaagc
     4141 attgccagcc acgtcaaata ctggctgcac aacggcttta tccgtgtgga cggcgaaaaa
     4201 atgtccaaat cgttgggcaa cttcttcact atccgcgaag tgttgaaaca atacgacccc
     4261 gaagtcgtgc gtttcttcat cctgcgcgcc cactaccgca gcccgttgaa ctattccgac
     4321 gcgcatttgg acgacgcaaa aggcgcactg actcgcctgt acactacatt gaaaaacacg
     4381 cctgccgccg cgtttgattt gtccgaaaac gccaacgact acacccgccg cttctacgcc
     4441 gccatgaacg acgatttcgg cacggttgaa gccgttgccg tgttgttcga actggcagga
     4501 gaagtgaaca aaaccaatga tgtacacctc gccggctgcc tgaaagcctt gggtggcatc
     4561 atcggcctgc tgcaacgcga tccgattgag ttcctgcaag gcggcgcggt ttcagacggc
     4621 ctctccaacg aagaaatcga cgatttgatt gcccgccgca aacaggcacg cgcggataaa
     4681 aactgggcgg aatccgacca tatccgcgac cttttgaacg aacacaaaat cattttggaa
     4741 gacaacgccg gcggcacgac ttggcggcgc ggttgaactg tatcggaaag ggcaaaagta
     4801 ataaacatgg aagatattat taataatatt aaaatcagat taaaagatct tggatttaac
     4861 ccggaagact ttctggaaat aaaaaaatat ctaatattat caataataag caaaataatt
     4921 ttaataggtg gatttatagt ttgcatagag tactctatga aattggatta aatgataaag
     4981 aattggatga cttattaaat aaattactag attatggaga ataagataag aggtgttttt
     5041 gtgaaaagta ccgccttttg ccctttttcc gttttcagac ggcatttccc gcttgaagcc
     5101 ccgccgtttt cagcaggcag ggccgaatgg gaaatgccgg gcggcgcacc gagtgccgct
     5161 ttgtgtatgg gaacattgtg agaacttatt tttattatgc ttaaaatcat ttccgccaac
     5221 gtcaacggca tccgctctgc ctacaaaaaa ggtttttacg aatatatcgc cgcatcgggc
     5281 gcggacattg tctgcgtgca ggaactcaaa gcgcaggaag cagatttgtc tgccgatatg
     5341 aaaaatccgc acgggatgca cggctattgg cattgcgccg agaagcgtgg ttacagtggc
     5401 gtggcggtgt acagcaaacg caaacccgac aatgtgcaaa tcggtatgga cattgaagaa
     5461 ttcgacaggg aagggcggtt tgtgcgttgc gatttcggca ggttgagcgt tatttcgctt
     5521 tatttgccca gcggcagcag cgcagaagaa cgccagcagg tgaaataccg tttcctcgat
     5581 gcgttttatc ctatgctcga agcgatgaaa aatgaagggc gcgacattgt cgtctgcggc
     5641 gactggaaca tcgcccacca aaacatcgac ctgaaaaact ggaaaggcaa tcagaaaaat
     5701 tcgggtttcc tgcccgaaga gcgcgagtgg ataggcaagg ttatccacaa actcggttgg
     5761 acggatatgt ggcgcacgct ttatcccgat gtgccgggct acacgtggtg gagcaaccgc
     5821 gggcaggcgt atgcgaaaga tgtcgggtgg cgcatcgatt atcagatggt tacgcccgaa
     5881 cttgccgcca aagccgtgtc cgcacacgtt tataaagatg aaaaattttc cgaccatgcg
     5941 ccgctggtcg tggagtatga ctatgctgcc gaataaagtt ttgggtaaat atgattggaa
     6001 tgtggacgga aaaacaggaa taggcgcggc atgggttgtc gctgcgttta tcctgcccat
     6061 attggtgtgg gcgatattta tgttgtcgcg gatgcaaggc tggcttgcgc cgaccaaggc
     6121 aaacccgata tgggcgttgg tgtggctgct gatctgcatg ccctgcctgc tgattgcgtc
     6181 caaatgtttg ggttggaaag gctggcggct gttggcaaac gttcttgcct gcctcgccgt
     6241 gtgcgctctg ttgagtgtgc ctgccgcgct gctgattgcg ttcacgctgc aagacctgct
     6301 caaataggcg cggacaatgg aaagtttcga gcttgaggat ttgacgctgt ggctggtacg
     6361 cgatgcggat gaagcggaaa tgtggataga ccgctgggcg attagttatc ccgtcgtgca
     6421 gatgtccgaa gcgtcggccg gtcaaagcat aggggaatgg caggcagggc ttcagacggc
     6481 atttgaacgc atacgcggca aatacgtcgc cgttgtcgca cacggtgcgg gcgcggccgc
     6541 attcttggca tggctgtatc aggcagacat cctgacacgg aagaaaattg ccaatatcat
     6601 ccttgtaccg cagcgtcccg atattttctc tgacgatgcg gaacacactt tccaacgcgt
     6661 ccgctgtcct tgccgtgccg cattggttgt acccgaacac ggcggcgtgc cgcacggttg
     6721 ggcgaaaaaa caggcggatt tgtggaacgc ccgcctgttg gtttccccgc attcgggcag
     6781 tttgaacggt atgctcggcg gctggcagtg gggtatgaag ctgatgcagg aaatgttgct
     6841 ggcgtgaacg cttgccttat cggaatttcg gctgtagcag gaagtccgca atcgggctgt
     6901 tttcattgac gtgcaggcgc atcaggttcg gataaagtcg ggctgccgca taccgccgga
     6961 aatggaatcc tttatttttt acgtcggcag tgccggcagg gcgttatccg gcagggttgc
     7021 aaggaaaatg ccgtctgaaa caggcttcag acggcatttc cattcaaaca gggcttacag
     7081 gctgcccaac acgatacgca atacgcggcg cagcggctcg gccgcgcccc acaagagctg
     7141 gtcgccgacg gtgaacgcgc tgatgtattc gccgcccatg cccagtttgc ggatgcgtcc
     7201 gacagggacg gacagcgtgc cggtaacttt ggcaggagtc agctcgtgga tgctggcttc
     7261 tttttcattg gggatgactt tcacccagtc attcgcgcct gccaaaatcg tttcgatttc
     7321 ggaaacaggc aggtcttttt tcaacttcag agtgatggct tggctgtggc agcgcatcgc
     7381 gccgacgcgg acgcacaggc cgtcaatcac ggttggattg tcgctgcggc cgaggatttt
     7441 gttggtttcc acgccgcctt tccattcttc tttggactgg ccgttgccca aatccacgtc
     7501 aatccacgga atcaggctgc cggcgagcgg tacgccgaag ttggctttcg gatagtcttc
     7561 gctgcgcagg aaatcggata ctttgcggtc gatgtcgaga atcgagccgg caggatcggc
     7621 aagttcgtcc gccacttggg cgtgaaccgc gcccataccg ctgatgagtt cgcgcatgtt
     7681 tttcgcacct gcgccggaag cggcttggta ggtcatgctg gttgcccatt cgaccaaatc
     7741 gttttggaac aggccgccca aagccatcag catcagggag acggtgcagt tgccgccgat
     7801 gtagtttttc acgccgtttt tgaggccgtt gtcgataacg ttgcggttga cagggtcgag
     7861 gacgataatc gcgtcgtctt tcatgcgcag agaagatgcc gcgtcaaccc agtagccgtt
     7921 ccagccgctg tcgcgcaggg cttggaagac ggatttggtg taatcgccgc cttggcaggt
     7981 aacgatgatg tccattttgg cgagttcggc aacattgttg gcatctaata atgttttagc
     8041 cgcctgaccg aaatcagggg ctgcgccgcc gacgttggaa gtggtaaaga aaaacgcttc
     8101 aggaatgtgg gcgaagtcgt tttcttcttt catacgctgc atcaaaaccg a
//
LOCUS       DAWHSK010000068         8099 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.068, whole genome shotgun sequence.
ACCESSION   DAWHSK010000068 DAWHSK010000000
VERSION     DAWHSK010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8099
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.068"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(220..2256)
                     /locus_tag="WIC03_000963"
     CDS             complement(220..2256)
                     /locus_tag="WIC03_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEZ3883486.1"
                     /translation="MTDNALLHLGEEPRFDQIKTEDIKPALQTAIAEAREQIAAIKAQ
                     THTGWANTVEPLTGITERVGRIWGVVSHLNSVADTPELRAVYNELMPEITVFFTEIGQ
                     DIELYNRFKTIKNSPEFDTLSPAQKTKLNHDLRDFVLSGAELPPEQQAELAKLQTEGA
                     QLSAKFSQNVLDATDAFGIYFDDAAPLAGIPEDALAMFAAAAQSESKTGYKIGLQIPH
                     YLAVIQYADNRELREQIYRAYVTRASELSDDGKFDNTANIDRTLANALQTAKLLGFKN
                     YAELSLATKMADTPEQVLNFLHDLARRAKPYAEKDLAEVKAFARESLNLADLQPWDLG
                     YASEKLREAKYAFSETEVKKYFPVGKVLNGLFAQIKKLYGIGFTEKTVPVWHKDVRYF
                     ELQQNGETIGGVYMDLYAREGKRGGAWMNDYKGRRRFSDGTLQLPTAYLVCNFAPPVG
                     GREARLSHDEILILFHETGHGLHHLLTQVDELGVSGINGVEWDAVELPSQFMENFVWE
                     YNVLAQMSTHEETGEPLPKELFDKMLAAKNFQRGMFLVRQMEFALFDMMIYSEDDEGR
                     LKNWQQVLDSVRKEVAVIQPPEYNRFALSFGHIFAGGYSAGYYSYAWAEVLSADAYAA
                     FEESDDVAATGKRFWQEILAVGGSRSAAESFKAFRGREPSIDALLRHSGFDNAA"
     gene            complement(2344..3216)
                     /locus_tag="WIC03_000964"
     CDS             complement(2344..3216)
                     /locus_tag="WIC03_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEZ3883487.1"
                     /translation="MEIWNMLDTWLGAVPIRAEAVESVAAVAALLLARALLLNIHFKR
                     HPDFGIESKRRFLVASRNITLLLVLFSLAFIWSAQIQTLALSMFAVAAAVVVATKELI
                     MCLSGSILRSATQQYSVGDYIEINGLRGRVVDINLLNTLMMQVGPNPLVGQLAGTTVS
                     FPNSLLLSHPVRRDNILGDYVIHTVEIPVPIHLDSDEAVCRLKAVLEPLCAPYIPAIQ
                     RHLENVQAEKLFITPAARPRVTRVPYDDKAYRIIVRFASPVSKRLEIQQAVMDEFLRV
                     QYRLLNYPAGSETL"
     gene            3486..5876
                     /gene="gyrB"
                     /locus_tag="WIC03_000965"
     CDS             3486..5876
                     /gene="gyrB"
                     /locus_tag="WIC03_000965"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004283954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEZ3883488.1"
                     /translation="MTEQKHEEYGADSIQVLEGLEAVRKRPGMYIGDTQDGSGLHHMV
                     FEVLDNAIDEALAGHCDKITVTIHADHSVSVADNGRGMPTGIHPKEGRSAAEVIMTVL
                     HAGGKFDNNSYKISGGLHGVGVSVVNALSDWVTLTIYRDGKEHFVRFVRGETEEPLNV
                     VGDSDKKGTTVRFLASTETFGNVEYSFDILAKRIRELSFLNNGVDIELTDERDGKHES
                     FALSGGVAGFVQYMNRKKTPLHEKIFYAFGEKDGMSVECAMQWNDSYQESVQCFTNNI
                     PQRDGGTHLTALRQVMTRTINSYIETNEVAKKAKVETAGDDMREGLTCVLSVKLPDPK
                     FSSQTKDKLVSGEIGPVVNEVISQALTDFLEENPAEAKIITGKIVDAARAREAARKAR
                     EITRRKGVMDGLGLPGKLADCQEKDPALSELYLVEGDSAGGSAMQGRDRKFQAILPLK
                     GKILNVEKARFEKMLASQEVAALITALGAGIGKEEFNAEKLRYHRIIIMTDADVDGAH
                     IRTLLLTFFYRQMPELVERGYIYIAQPPLYKAKYGKQERYLKDELEKDQWLLGLALEK
                     AKIVSDGRTIEGAELADTAKQFLLAKTVIEQESRFVDELVLRAMLHASPIDLTSSENA
                     DKAVAELSGLLDEKEVALERIEGHEGHRFIKITRKLHGNVMVSYIEPKFLNSKAYQTL
                     TQTAAALKGMVGEGAKLYKGENGYDADSFETALDILMSVAQKGMSIQRYKGLGEMNPE
                     QLWETTMDPAVRRLLKVRIEDAIAADEVFVTLMGDEVEPRRAFIENNALIAQNIDA"
     gene            5975..7360
                     /locus_tag="WIC03_000966"
     CDS             5975..7360
                     /locus_tag="WIC03_000966"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HEZ3883489.1"
                     /translation="MVSIFDIFKIGIGPSSSHTVGPMKAAAAFAAGLDAQAVRIVIDI
                     YGSLALTGYGHGTFDALMLGLEGSLPHDIPLVGIPERLERIRTQHILRLNGQEIRFIP
                     DRDLNILGNQVLPKHPNGLRFTAYASDGTVLNEQVYYSVGGGFVVTEEDFDRQAETEK
                     AVPYPYTSCAELLARCRLNRLDISEVVLANEAALAGCGEAEIRRRAAAVAEVMEGCIK
                     RGLGADGELPGGLNVRRRAPQLAAKLKVLRETEIVNTQLWPMVYAMAVNEENAAGGRV
                     VTAPTNGAAGIIPAVLHYFRKFNPHATQERVENFLLTAGAIGILYKTNASISGADVGC
                     QGEVGVACSMAAGAYAEVIGGTPKQVENAAEMAMEHHLGLTCDPVGGLVQIPCIERNG
                     IAAEKALKLGTLALLEDGTDKKVSLDEVIRTMLQTGRDMKATYKETSLAGLAATLRKK
                     AVPVSVRVVEC"
     gene            complement(7431..7529)
                     /locus_tag="WIC03_000967"
     CDS             complement(7431..7529)
                     /locus_tag="WIC03_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="eamA-like transporter family protein"
                     /protein_id="HEZ3883490.1"
                     /translation="MAVVFLDERPNTQEWIGLGLVTAGVLTLALKR"
BASE COUNT         1884 a         2245 c         2237 g         1733 t
ORIGIN      
        1 ccgtctgaag cgttgtttga ccgtttcaga cggcattttt atcaaattca aaaatattcc
       61 gtcattcccg cgaaagcggg aatctagaac gtaaaatcta aagaaaccgt tttatccgat
      121 aagtttccgt gccgaccggt ctggattccc gcctacgcgg gaatgacggt gcggatgttt
      181 ttatatcgaa tcctcctatt ttttacttca cccttgccgt caggccgcgt tgtcgaaacc
      241 gctgtggcgc aagagtgcgt ctatgctcgg ttcgcggccg cggaaggctt tgaaggattc
      301 tgccgcgctg cgcgatccgc cgacggcgag gatttcctgc caaaagcgtt tgcctgtggc
      361 ggcgacatcg tcgctttctt caaaggcggc gtatgcgtcc gcgctcaata cttccgccca
      421 cgcgtagctg taatagcctg cggaatagcc gcctgcgaag atgtggccga agctcaaggc
      481 gaagcggttg tattcgggcg gttggatgac ggcgacttct ttgcgcacgc tgtctaaaac
      541 ctgttgccag tttttcagac ggccttcgtc gtcttcgctg taaatcatca tatcgaagag
      601 ggcgaactcc atctggcgga cgaggaacat tccgcgttgg aagtttttgg cggcgagcat
      661 tttgtcgaag agttctttcg gcaggggctc gccggtttct tcgtgggtgg acatttgcgc
      721 caatacattg tattcccaaa cgaaattttc cataaactgg ctgggcagtt cgaccgcgtc
      781 ccattctacg ccgttgatac cggacacgcc cagttcgtcc acttgggtaa gcaggtggtg
      841 cagcccgtgt ccggtttcgt ggaagaggat gaggatttcg tcgtggctca aacgcgcttc
      901 tctgccgccg acgggcgggg cgaagttgca gacgaggtag gcggtgggca gttgcagcgt
      961 gccgtctgaa aaacggcggc gacctttgta gtcgttcatc cacgcgccgc cgcgtttgcc
     1021 ttcgcgtgcg tacaaatcca tataaacgcc gcctatggtt tcgccgtttt gttgcaattc
     1081 aaaatagcgc acgtctttgt gccagacggg gacggttttt tcggtaaatc cgatgccgta
     1141 gagttttttg atttgggcga acagtccgtt taatactttg ccgacgggga agtatttttt
     1201 gacttcggtt tcgctgaacg cgtatttggc ttcgcgcagt ttttcgctgg cgtagcccaa
     1261 gtcccacggt tgcaaatcgg cgaggttcag gctttcgcgg gcgaaggctt tgacttcggc
     1321 gaggtctttt tcggcgtagg gtttggcgcg gcgggcgagg tcgtgcagga agtttaaaac
     1381 ttgttcgggc gtgtccgcca ttttggttgc cagcgacaat tcggcgtagt ttttgaagcc
     1441 gagcagtttg gcggtttgca gggcgtttgc gagcgtgcgg tcgatgttgg cggtgttgtc
     1501 gaatttgccg tcgtctgaaa gttcgctggc gcgggtaacg taggcgcggt agatttgttc
     1561 gcgcagttcg cggttgtcgg cgtattggat gacggcgagg tagtgtggaa tctgcaagcc
     1621 gattttgtag cctgttttgc tttcgctttg cgcggcggcg gcaaacatgg cgagcgcgtc
     1681 ttcgggaatg ccggcaagcg gtgcggcatc gtcaaagtaa atgccgaacg cgtcggtcgc
     1741 gtctaggacg ttttgggaga atttggcgga aagttgcgcg ccttcggttt gcagttttgc
     1801 cagttctgcc tgctgttcgg gcggcagttc cgcgccgctg aggacgaaat cgcgcagatc
     1861 gtggttgagt ttggtttttt gcgcggggga gagggtgtcg aactcggggg agtttttgat
     1921 ggttttgaag cggttgtaca gctcgatgtc ttgtccgatt tcggtgaaga agacggtgat
     1981 ttcgggcatc agttcgttat agacggcgcg cagttcgggc gtgtcggcga cggagttgag
     2041 gtgcgacacc acgccccaaa tcctgccgac gcgttcggtg atgccggtca ggggttcgac
     2101 agtgtttgcc cagccggtgt gcgtttgggc tttgatggcg gcgatttgtt cgcgcgcttc
     2161 ggcaatggcg gtttgcaggg cgggtttgat gtcttcggtt ttgatttgat caaaacgggg
     2221 ttcttcgccc aaatggagca gtgcgttgtc agtcataaga tgggtttcct ttgcgtgttg
     2281 tgcttcagac agcatatcgg caatatgccg tctgaagccg gaaatggggt cggtcaggga
     2341 aagttaaagt gtttcggagc cggcggggta atttaacagg cggtattgta cgcgcaaaaa
     2401 ttcgtccata accgcctgtt ggatttccag ccgctttgaa acgggggagg cgaagcggac
     2461 gatgatgcgg tatgccttgt catcgtacgg cacgcgggta acgcgcggtc tggcggcggg
     2521 cgtgataaac agtttttccg cctgcacgtt ttccaaatgc cgttggatgg cggggatgta
     2581 gggcgcgcac aagggctcga gtacggcttt cagacggcat acggcttcat ccgaatccaa
     2641 atggatggga acggggattt cgaccgtatg gatgacatag tcgcccaaaa tattgtcgcg
     2701 gcgcacgggg tggctcaaca acaggctgtt ggggaaagaa acggtggttc ccgcaagctg
     2761 tccgaccaag gggttcggac cgacctgcat catcagcgtg ttcaacaggt tgatgtcgac
     2821 cacgcgcccg cgcaggccgt tgatttcgat atagtcgccg accgagtatt gctgggtggc
     2881 agaccttaaa atactgcccg acagacacat aatcagttcc ttcgtcgcca cgacgaccgc
     2941 cgccgccacc gcaaacatcg acaaagccag cgtttggatt tgcgccgacc agataaatgc
     3001 cagcgaaaac agcaccaaaa gcagcgttat attgcggctg gcaaccaaaa accgccgctt
     3061 gctttcgatg ccgaaatccg gatgccgttt gaagtggata ttcaacagaa gggcgcgcgc
     3121 cagcagcaaa gccgcaaccg ccgccacgga ttcgaccgcc tccgcacgta tcgggacggc
     3181 accgagccaa gtgtccaaca tattccatat ttccatttcc gcgcccctgt ccgaaaagta
     3241 tagggtgtag attttagtgg caaaaaaacg cttttgccac tttacgggaa aatccccgac
     3301 ctgatgccgg aggcaaacgt accgtttcat cttttcccgg cggcacactg atgcggataa
     3361 cgctgaatta ggcggcaaaa tcggctataa tacacaatta tatatgccgt cccgcccgct
     3421 ttttccacaa ggtgcggact tgttttggcg gcatcgcaaa atcttttcaa aatccggcaa
     3481 aaaatatgac tgaacaaaaa cacgaagaat acggcgccga cagcattcag gtgctcgaag
     3541 gcttggaagc ggtacgcaaa cgccccggca tgtacatcgg cgacacgcag gacggcagcg
     3601 gtctgcacca tatggtgttt gaagtattgg acaacgccat tgacgaagca ctcgccggac
     3661 attgcgacaa aatcacggta acgatacacg ccgaccattc cgtcagcgtc gccgacaacg
     3721 ggcgcggtat gcccaccggc atccacccga aagaaggacg ctccgccgcc gaagtcatca
     3781 tgaccgtatt gcacgcgggc ggtaaattcg acaacaacag ctacaaaatc tccggcggcc
     3841 tgcacggcgt gggcgtgtcc gtcgtcaacg cgctgtccga ctgggtaacg ctgaccatct
     3901 accgcgacgg caaagaacac ttcgtccgct tcgtgcgcgg cgaaaccgaa gagccgctga
     3961 acgttgtcgg cgattccgat aaaaaaggca cgaccgtgcg cttcctcgcc agtacggaaa
     4021 ccttcggcaa cgtcgaatac agcttcgaca tccttgccaa acgcatccgc gaactttcct
     4081 tcctgaacaa cggcgtggac atcgaattga ccgacgagcg cgacggcaaa cacgaaagct
     4141 tcgccctttc cggcggcgtg gctggattcg tgcaatacat gaaccgcaaa aaaacgccct
     4201 tgcacgaaaa aatcttctac gcgttcggcg agaaagacgg catgagcgtc gaatgcgcga
     4261 tgcaatggaa cgacagctat caggaaagcg tgcagtgctt caccaacaac atccctcagc
     4321 gcgacggcgg tacgcacctg accgcgttgc gccaagtgat gacgcgcacc atcaacagct
     4381 acatcgaaac caacgaagtc gccaaaaaag ccaaagtgga aaccgccggc gacgatatgc
     4441 gcgaaggttt gacctgcgtg ttgtccgtca aactgcccga ccccaaattt tcgtcccaaa
     4501 ccaaagacaa actggtttcc ggcgaaatcg gtcccgtcgt caacgaagtc atcagccaag
     4561 ccctgaccga cttcctcgaa gaaaaccctg ccgaagccaa aatcatcacc ggcaaaatcg
     4621 tcgatgccgc ccgcgcgcgc gaagccgccc gcaaagcccg cgaaatcacc cgccgcaaag
     4681 gcgtgatgga cggtttgggc ctgccgggca aactcgccga ctgccaagaa aaagaccccg
     4741 cattgtcaga actttatctg gtcgagggcg attctgcagg cggttccgcc atgcaggggc
     4801 gcgaccgcaa attccaagcg attttgccgc ttaagggcaa gattttgaac gtcgaaaaag
     4861 cgcgctttga aaaaatgctc gccagtcagg aagtagccgc tttgattacc gcgttgggcg
     4921 cgggcatcgg caaagaagaa ttcaatgccg agaagctgcg ctaccaccgc atcatcatca
     4981 tgaccgatgc cgacgtggac ggcgcgcaca tccgcaccct gctcctgacc ttcttctacc
     5041 gccaaatgcc cgaactggtc gagcgcggct acatctatat cgcccagccg cctttgtata
     5101 aagcgaaata cggcaaacag gaacgttacc tcaaagacga actggaaaaa gaccaatggc
     5161 tgctcggtct tgccttggaa aaagccaaaa tcgtttcaga cggccgcacc atcgaaggcg
     5221 cagaacttgc cgacaccgcc aaacaattcc tgttggcaaa aaccgtcatc gaacaggaaa
     5281 gccgcttcgt agacgaactc gtcctgcgcg ccatgctaca cgcgtcgccc attgatttga
     5341 cgtcgtctga aaacgccgat aaagccgttg ccgaactttc cggtctgctt gacgaaaaag
     5401 aagtcgccct cgaacgcatc gaaggtcatg aaggacaccg gttcatcaaa atcacgcgca
     5461 agctgcacgg caacgtcatg gtcagctaca tcgaacccaa gttcctcaac agcaaagcct
     5521 accaaaccct cacccaaacc gccgccgcgc tcaaaggcat ggtcggcgag ggtgccaagc
     5581 tttacaaagg cgaaaacggg tacgacgcgg acagctttga aaccgctttg gacatcttga
     5641 tgagcgttgc ccaaaaaggt atgtccatcc aacgatacaa aggcttgggc gagatgaacc
     5701 ccgagcagct gtgggaaacc acgatggatc ccgccgtgcg ccgtctgttg aaagtgcgca
     5761 tcgaagacgc cattgccgcc gacgaagtat tcgttaccct gatgggcgac gaggtcgaac
     5821 cgcgccgcgc ctttatcgaa aacaacgcgc tgattgccca aaatatcgac gcataagtgc
     5881 cgttttaaaa aaggagacgg gcatcgtgcc gcgtctcctt ttggtttgtc aaacggaagc
     5941 cataccgtct gaaaccgcct ttcggagcaa aaccatggtc agcatttttg atattttcaa
     6001 aatcggtatc gggccttcca gttcgcatac ggtcggcccg atgaaggcag ccgccgcctt
     6061 tgcggcaggt ttggatgcac aggctgttcg catcgtcatc gacatttacg gctcgctcgc
     6121 cctgaccgga tacggacacg gtacatttga cgcactgatg ctcggtttgg aaggcagcct
     6181 gccgcacgac atcccgcttg tcggcattcc cgaacgcctc gaacgcatcc gcacgcagca
     6241 catcctccgg ctcaacgggc aagaaatccg cttcatcccc gaccgcgacc tgaacatact
     6301 cggcaatcaa gtgctgccca aacaccccaa cggcctgcgt tttaccgcct atgcttcaga
     6361 cggcacggta ttgaatgaac aggtttatta ttcggtcggc ggcggctttg tcgttaccga
     6421 agaagatttt gaccggcagg cggaaacgga aaaagccgtt ccctatccct ataccagttg
     6481 cgccgaactg cttgcccgat gccgtctgaa ccggctcgac atctccgaag tcgtgttggc
     6541 aaacgaagcc gcgcttgccg gatgcggcga agccgaaatc cgccgccgcg ccgctgccgt
     6601 tgccgaggtt atggaaggct gcatcaaacg cggcttgggt gcggacggcg aactgcccgg
     6661 cggattgaac gtccgccgcc gcgccccgca gcttgccgcc aagctcaaag tcctgcgcga
     6721 aaccgaaatc gtcaacaccc agctctggcc gatggtgtac gccatggcgg tcaacgaaga
     6781 aaacgccgcc ggcggacgcg tcgttaccgc accgaccaac ggcgcggcag gcatcattcc
     6841 cgccgtattg cactatttcc gcaagttcaa tccgcacgcc acacaggaac gcgtcgaaaa
     6901 cttcctgctc accgcaggcg caatcggcat cctctacaag accaacgcct ccatttccgg
     6961 tgcggatgtc ggctgtcagg gcgaagtcgg cgtagcgtgt tcgatggcgg cgggcgcata
     7021 cgccgaagtc atcggcggca cgcccaaaca agtggaaaac gccgccgaaa tggcgatgga
     7081 acaccatttg gggctgactt gcgaccccgt cggcggactg gtgcaaatcc cctgcatcga
     7141 gcgcaacggc atcgccgccg aaaaagccct caaactcggc acgctcgcgc ttttggaaga
     7201 cggcacggac aaaaaagtct cgctcgacga agtcatccga accatgctgc aaaccggccg
     7261 ggatatgaag gcgacctaca aagaaacctc gcttgccgga ctcgccgcca cgctccggaa
     7321 aaaagccgtc cccgtatccg tgcgcgtggt cgagtgctga ggatggacag aaacgaaaat
     7381 gccgtctgaa aacagttttt tagacggcat ttcatttatt tcttgcggct ttaccgtttc
     7441 agtgccagcg tcaacacgcc cgccgtaacc agccccaagc ctatccattc ctgcgtgttc
     7501 gggcgttcgt ctaagaaaac caccgccatc aggaatgcaa accggcgttg tttgcagcat
     7561 ccggcaagaa tttttatagt ggattaacaa aaatcaggac aaggcgacga agccgcagac
     7621 agtacaaata gtacggaacc gattcacttg gtgcttcagc accttagaga atcgttctct
     7681 ttgagctaag gcgaggcaac gctgtactgg tttttgttaa tccactataa aaggtctttt
     7741 ctaaaatatg cagatattcg aatatcgaat ttgccttatg attacggttt tctgtcttat
     7801 ctgccttaaa acgccggtat tccgtttgaa ccaaattata tttgccgaaa cactcaaaaa
     7861 tctcccttac ctgcccgaca gacatcaagc cttcgttgtt gtagctgaga aaaatatatt
     7921 gaaagtccgc atccctaatc aaagcctcaa ataccggaac cacatccgat tttgagcaag
     7981 tccgcgctaa aacacatcgt acagcgcaaa accggatggg aatggcaaaa tccggaaaca
     8041 aacatttttc aaagcataca aacaaaaaaa taccaaccgg aggttggtat tccttaact
//
LOCUS       DAWHSK010000069         8089 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.069, whole genome shotgun sequence.
ACCESSION   DAWHSK010000069 DAWHSK010000000
VERSION     DAWHSK010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8089
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.069"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(63..623)
                     /locus_tag="WIC03_000968"
     CDS             complement(63..623)
                     /locus_tag="WIC03_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HEZ3883491.1"
                     /translation="MNMKTLLALAVSAVCSVGVAQAHEHNTIPKGASIEVKVQQLDPV
                     NGNKDVGTVTITESNYGLVFTPDLQGLSEGLHGFHIHENPSCEPKEKEGKLTAGLGAG
                     GHWDPKGAKQHGYPWQDDAHLGDLPALTVLHDGTATNPVLAPRLKHLDDVRGHSIMIH
                     TGGDNHSDHPAPLGGGGPRMACGVIK"
     gene            complement(1040..2080)
                     /gene="mutY"
                     /locus_tag="WIC03_000969"
     CDS             complement(1040..2080)
                     /gene="mutY"
                     /locus_tag="WIC03_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEZ3883492.1"
                     /translation="MNTPIPFSERLIRWQKQHGRHHLPWQVKNPYCVWLSEIMLQQTQ
                     VATVLDYYPRFLEKFPTVQTLAAAPQDEVLSLWAGLGYYSRARNLHKAAQQVVRQFGG
                     TFPSERKDLETLCGVGRSTAAAICAFSFNRRETILDGNVKRVLCRVFARDGNPQDKKF
                     ENSLWTLAESLLPSENADMPAYTQGLMDLGATVCKRTKPLCHQCPMADICEAKKQNRT
                     AELPRKKTAAEVPTLPLYWLIIRNRNGAILLEKRPAKGIWGGLYCVPCFESLNGLSDF
                     AAKFSLTMADMDEQTALTHRLTHRLLMITPFEGQMPSESPSDGIWIKPTHLKDYGLPK
                     PLEIYLNGNRLE"
     gene            2243..3284
                     /locus_tag="WIC03_000970"
                     /pseudo
     CDS             2243..3284
                     /locus_tag="WIC03_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225132.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
     gene            complement(3307..3525)
                     /locus_tag="WIC03_000971"
     CDS             complement(3307..3525)
                     /locus_tag="WIC03_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883493.1"
                     /translation="MPSEALSSLRRHFAAWRRSRHTRQHHSDILPLKCPSAFRIIKIT
                     FSSFDSSRTAHTTRLKKCRLKRIQTASA"
     gene            complement(3540..4178)
                     /locus_tag="WIC03_000972"
     CDS             complement(3540..4178)
                     /locus_tag="WIC03_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEZ3883494.1"
                     /translation="MSKLTPREILTAGAVVPVMAIDDLSTAIDLSHALVEGGIPTLEI
                     TLRTPVGLDAIRLIAKEVPNAIVGAGTVTNPEQLKAVEDAGAVFAISPGLHESLAKAG
                     RNSGIPLIPGVATPGEIQLALEHGIDTLKLFPAEVVGGKAMLKALYGPYADVRFCPTG
                     GISLATAPDYLALPNVLCVGGSWLTPKEAVKNKDWDTITRLAKEAAALKPKA"
     gene            complement(4359..6194)
                     /gene="edd"
                     /locus_tag="WIC03_000973"
     CDS             complement(4359..6194)
                     /gene="edd"
                     /locus_tag="WIC03_000973"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HEZ3883495.1"
                     /translation="MNTTPIHSKLAEITGRIIERSRPTREKYLAKIRSAKQMGRLERN
                     QLGCSNLAHGYAAMPKSIKIEMLQETVPNLGIITAYNDMVSAHQPFKDFPDQIKDEAQ
                     KNGATAQVAGGTPAMCDGITQGYAGMELSLFSRDVIAMSTAVGLSHQMFDGSLFMGVC
                     DKIVPGLMIGALSFGHIPGIFVPAGPMSSGIGNKEKARTRQLFAEGKVGRNELLKSEM
                     GSYHSPGTCTFYGTANSNQMMMEMMGVHLPAAAFVHPYTDLREALTRYAAGHLARGIK
                     NGTIKPLGEMLTEKSFINALIGLMATGGSTNHTMHLVAMARAAGVILNWDDFDEISSI
                     IPLLIRVYPNGKADVNHFTAAGGLPFVIRELLNAGLLHDDVDTVVGHGMRHYTKEPFL
                     IDGKLEWREAPETSGNDDILRKADNPFSPDGGLRLMKGNIGRGVIKVSAVREGCRIIE
                     APAIVFNDQREVLAAFERGELERDFVCVVRYQGPRANGMPELHKLTPPLGILQDRGFK
                     VALLTDGRMSGASGKVPASIHMTPEALMGGNIAKIRTGDLIRFDSVSGELNVLINETE
                     WNAREVESIDLGANQQGCGRELFANFRSMTSSAETGAMSFGGEFA"
     gene            6710..>8089
                     /gene="zwf"
                     /locus_tag="WIC03_000974"
     CDS             6710..>8089
                     /gene="zwf"
                     /locus_tag="WIC03_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEZ3883496.1"
                     /translation="MSTQTNFDLVLFGATGDLAMRKLLPCLYQAHVAGLLHPEGRILG
                     VSRSELDTEGFLAKVETSSKIHVKENFSDEAWASFVERFAYLKVDVTQPDDFAALGDL
                     VKARKETDNVVIYLSTAPKFFAQTCENLAKIGLNADNVRVVLEKPLGTDLASSQQINT
                     DVARYFKEGQIYRIDHYLGKESLQNLLALRFANVMFEPLWNNKYIESVQLTIAEQLGV
                     EERGEFYDITGALRDMVQNHLMQMLCMTAMEAPASLDADAVRDEKVKVIKSLKPLTVE
                     SVNENVVRGQYTAAKGMNGYLEEINVPQDSFTETYVAIKAEIENERWKGVPFYLRTGK
                     RMAGKVAEIVLNFKDLNSHIFEGSRTAPNRLVIELQPYESVRLYTQMKTPGAGNKVET
                     VPLATDLGKALESRRAEAYERLLLDVINGKLALFNRRDELEAAWEYVMPILENWTNNT
                     TPPHGYGAHS"
BASE COUNT         1880 a         1939 c         2221 g         2049 t
ORIGIN      
        1 ctaaatatct agcaaaaaag tgcggtcaaa attcaccgca cttttcgttt cgaacaatcg
       61 aattatttaa tcacgccaca tgccatacgt gggccgccac cgccaagtgg agctggatga
      121 tcggagtgat tatcaccacc cgtgtggatc ataatagagt gaccgcgaac atcatctaaa
      181 tgtttaagac gtggtgctaa aacaggattt gttgctgtgc catcatgcaa tacagttaat
      241 gcaggtaaat cacctaagtg tgcatcatct tgccatgggt aaccatgttg ttttgcacct
      301 ttaggatccc agtgaccgcc tgcgcctaaa ccagctgtca atttaccttc tttttctttt
      361 ggctcacagc ttgggttttc atggatgtgg aaaccatgta agccttcgct taatccttgt
      421 aaatcagggg taaacacaag accatagtta gattcagtaa tagtcactgt acccacatct
      481 ttgttaccgt ttactggatc aagttgttgc actttcactt caatagaagc acctttaggt
      541 atcgtattat gctcgtgtgc ttgcgcaaca ccaactgaac atactgcact aaccgctagt
      601 gctaataagg ttttcatatt cattgcaatc tcctttattg cagtaaaaac tcgcgactat
      661 tttagtcaat tttgttttaa aagccgataa atttttacga aaattttaca ttaattgtta
      721 tttgcagaaa attagaaccc gttatcacgt cttggataaa tgccaaaacc attgtgattc
      781 agagaattaa aaagaaaaat ttcggaatcc tcatcctttt ctgaaacctg attgcggtat
      841 ccatggcttt gtctttgtca tttaagtttg tgttcatgtt tttgaccttc tttgcttttt
      901 aaatcctttt taaattgccg acttcttccg ttccaaatgt cccatttgaa cattatcccg
      961 ccgcctaaaa tacgacccaa attcatcggt tgttcttgct gcgatacctg caaacaacag
     1021 ttcaatgggt ttattttgtt tattctaacc tattaccgtt taaataaatt tccaaaggct
     1081 tgggcaggcc gtaatctttc aaatgcgtcg gctttatcca aatgccgtct gaagggcttt
     1141 cagacggcat ttgtccttca aacggcgtaa tcatcagcaa ccggtgcgtc agccggtggg
     1201 tcagggcggt ttgttcgtcc atatctgcca tggtcaggga gaatttggcg gcaaagtcgg
     1261 aaagcccgtt caaactttca aaacacggca cgcaatacag cccgccccaa atgcctttgg
     1321 cggggcgttt ttccagcaaa atcgcgccgt tccggttgcg gataatcagc cagtaaagcg
     1381 gcagggtcgg tacttcggcg gcggtttttt tgcgcggcag ctcggcggtg cggttttgct
     1441 tttttgcttc gcagatgtcc gccatcgggc attggtggca caagggtttc gtccgtttgc
     1501 acacggttgc gcccaaatcc atcaaacctt gtgtataggc aggcatatcg gcgttttcag
     1561 acggcagcag gctttcggca agtgtccaga gcgagttttc aaattttttg tcctgcggat
     1621 tgccgtcgcg ggcgaacacg cggcagagta cgcgtttgac gttgccgtcc aaaatggttt
     1681 cgcggcggtt gaaggagaag gcgcaaatgg cggcggcggt gcttctgcct acgccgcaga
     1741 gcgtttccaa gtctttgcgt tccgatggaa acgtgccgcc gaattgcctg acgacttgtt
     1801 gcgcggcttt gtgcaggttg cgcgcgcggc tgtaatagcc caagcccgcc cacaacgaca
     1861 acacttcgtc ttgcggcgcg gcggcaagcg tctgaacggt cgggaatttt tctaagaaac
     1921 gcggatagta gtccaacacg gtggcgacct gcgtttgctg gagcatgatt tcggaaagcc
     1981 agacgcaata agggtttttg acctgccaag ggaggtggtg gcgaccgtgt tgtttttgcc
     2041 agcggatgag ccgttcggag aaggggatgg gtgtgttcat taatatcaat cggtggtttt
     2101 atttatattt aaaacagtat gttattgtat aacattgtga aaataattct tattgactta
     2161 ttttttgtag gggtatataa ctcatataaa gaaactttat tgcggtattg aaattattta
     2221 tcaacaagca aggagtatca gcatgaaagc aatggtttat cacggcgcaa acgacatccg
     2281 ttttgaagaa aaaccccgtc cgcagattat cgatccgacc gatgcggtgg tgaaaatcgt
     2341 caaaaccacg atttgcggta ccgatttggg tatttggaaa ggcaaaaaaa cccgaagtcg
     2401 ccgacggtcg tattctcggt catgaaggca tcggtattgt agaagaagtc ggcgatgctg
     2461 taaaaaacat caaagtcggc gataaagtca ttatttcatg cgtcagcaaa tgctgtactt
     2521 gcgacaactg caaaatccaa ctttattcgc attgccgcaa cggcggctgg attttgggct
     2581 acatgattga cggcacgcag gcagaatacg tccgcacgcc ttatgccgac aacagcctcg
     2641 tgccgctgcc cgacaacgtc aacgaagaag tcgccctgtt gttgagcgac gctttgccga
     2701 ccgcacacga aatcggcgtg caatacggcg atgtcaaacc cggcgacacc gtattcatcg
     2761 caggtgccgc tcctgtcggt atgtccgcct tgttgaccgc ccaactgtat agccccgccg
     2821 ccattatcgt gtgcgatatg gacgaaaacc gtttgaaact ggcgaaagag ttgggcgcga
     2881 cccataccat cagccccgct tccggcgacg tctccaaaca agttttcgcc atcgtcggcg
     2941 aagacggcgt agattgtgcc atcgaagcgg tcggtatccc tgcaacttgg aatatgtgcc
     3001 aagacatcgt gaaacccggc ggccatatcg ccgttgtcgg cgtacacggt caatccgttg
     3061 atttcaaact ggaaaaactg tggattaaaa agcttgccat cactaccggc ctggtaaacg
     3121 ccaataccac cgaaatgctg atgaaggcaa tttccagcag ctccgtcgat tacaccaaaa
     3181 tgttgaccca ccgtttcaaa ttcagcgaat tggaaaaagc ctacgacgtg ttcaaacacg
     3241 ccgccgaaaa tcaagcgatg aaagtggttt tggaagcgga ttaatcggcg ttgaatcagt
     3301 tgttttttaa gcggatgccg tctgaatgcg tttcagacgg cattttttca gccgcgtagt
     3361 atgggctgtc cggctgctgt cgaatgaact gaacgttatt tttattatgc ggaaggcgga
     3421 tgggcatttt aggggcagta tgtcggagtg gtgctgccgt gtatgccgac tgcgccgcca
     3481 agcggcaaaa tgccgtctga gactggaaag ggcttcagac ggcattttta cgatgcggtt
     3541 caggctttgg gtttcaatgc cgccgcttct ttggcgagac gggtgatggt gtcccagtct
     3601 ttgtttttca cggcttcttt cggcgtcagc catgagccgc cgacgcacag gacgttgggc
     3661 agtgccaagt aatcgggcgc ggtggcgagg ctgatgccgc ctgtcgggca gaagcgaaca
     3721 tcggcgtaag ggccgtacag ggctttgagc atggctttgc cgccaacgac ttcggcaggg
     3781 aagagtttga gggtgtcgat gccgtgttcc aaagccagtt ggatttcgcc cggggtggca
     3841 acaccgggaa tcagggggat gccgctgttg cggccggctt tggcgaggga ttcatgcagc
     3901 cccgggctga tggcgaaaac cgcgcctgcg tcttcgacgg ctttaagctg ttcgggattg
     3961 gttaccgtac ctgcgccgac gatggcgttg ggcacttctt tggcaatcag gcggatggca
     4021 tcgaggccga caggggtgcg cagggtgatt tcgagggtag ggatgccgcc ttcgacaagg
     4081 gcgtgggaca aatcgatggc ggtgcttaag tcgtcaatcg ccattaccgg cacaactgcg
     4141 ccggcggtca aaatttcgcg gggggtcagt ttggacattt cggttctccg ggtggaatgg
     4201 ggtattttat taagatggga caggtcgtag gtcggattca agaaaccgac atcttggacg
     4261 gctgtgtcgg attcgagaat ccaacctacg tctatcttaa aacacttgaa taatttttca
     4321 gacggccttc ggtctgaaaa ggccgtctga aacgcgcatc aggcaaattc gccgccgaag
     4381 ctcatcgcgc cggtttcggc gctgctggtc atgctgcgga agttggcgaa aagttcgcgg
     4441 ccgcagcctt gttggttcgc gcccaagtcg atgctttcga cttcgcgggc attccattcg
     4501 gtttcgttaa tcaggacgtt gagttcgccg ctaacggagt cgaagcggat caggtcgccg
     4561 gtacggattt tggcgatgtt gccgcccatc agggcttcgg gcgtcatgtg gatggaggcc
     4621 ggaactttgc cggatgcgcc ggacatacga ccgtcggtca gcagtgccac tttgaagccg
     4681 cggtcttgca ggatgcccaa aggcggggtc agtttgtgca attcgggcat accgttggca
     4741 cgcgggcctt ggtagcggac gacgcacaca aaatcgcgtt ccaactcgcc gcgttcaaac
     4801 gcagccaaca cttcgcgttg gtcgttgaac acgatggcag gcgcttcaat aatgcggcag
     4861 ccttcgcgca cggcggacac tttaatcacg ccacgtccga tgttgccttt catcaggcgc
     4921 agaccgccgt cgggggagaa cgggttgtca gctttacgca ggatgtcgtc gttgccgctg
     4981 gtttcggggg cttcgcgcca ttcgagtttg ccgtcgatga ggaaaggctc tttggtgtag
     5041 tggcgcatac cgtgtccgac gacggtatcg acatcgtcgt gcaacaggcc tgcattcagc
     5101 aattcgcgga taacgaaagg cagtccgccc gctgcggtaa agtgattcac gtcggccttg
     5161 ccgttcggat aaacgcggat gagtagtggg atgatggagg aaatttcgtc gaagtcgtcc
     5221 cagttcaaaa tcacgccggc cgcacgcgcc atagcgacga ggtgcatggt gtggttggtc
     5281 gaaccgccgg ttgccatcag gccaatcaag gcgttgataa aggatttttc ggtcaacatt
     5341 tcgcccaaag gtttaatcgt gccgtttttg atgccgcgcg cgaggtgtcc ggcggcgtag
     5401 cgagtcagcg cttcgcgcag gtcggtgtaa gggtggacga aggcggcggc aggcaggtgc
     5461 acgcccatca tttccatcat catttggttg gagtttgccg tgccgtagaa agtgcaggtg
     5521 cccgggctgt ggtaagaacc catttcgctt ttcaaaagtt cgttgcgtcc gaccttgcct
     5581 tcggcgaaaa gctggcgggt gcgggctttt tctttgttgc cgataccgct ggacatcggg
     5641 cctgcgggga cgaagatacc cggaatatga ccgaacgaaa gcgcgcctat catcaaaccc
     5701 ggaacgattt tgtcgcatac gcccataaac aggctgccgt caaacatttg atgcgacagc
     5761 ccgacggcgg tactcatggc aatcacgtcg cgggagaaca gcgacagttc catgccggcg
     5821 tagccttgcg tgatgccgtc gcacatggcg ggcgtgccgc cggcgacttg ggcggtcgcg
     5881 ccgtttttct gcgcttcgtc tttgatttgg tcagggaagt ctttaaacgg ctggtgtgcg
     5941 gaaaccatgt cgttgtaggc ggtgatgatg cctaagttgg ggacggtttc ctgaagcatt
     6001 tcgattttga tacttttagg catggcagca tagccgtgtg ccaagttgct gcagccgagc
     6061 tggttgcgct ctaagcgtcc catttgtttg gcactgcgga tcttcgccag atatttttca
     6121 cgcgtcggac ggctgcgttc aataatgcgc ccggtgattt cggcgagttt ggagtggata
     6181 ggagtagtgt tcacggtcgg tctcctgtcg gaacgggggg tattggggat ttgtttgtgc
     6241 gtttctgatt tttgtatcgg gtattcagac ggtaagtata gtggattaac aaaaaccagt
     6301 acggcgttgc ctcgccttgc cgtattatct gtactgtctg cggcttcgtc gccttgtcct
     6361 gatttttgtt aatccactat attttaaaag gtatgccgtc tgaaagtggg aagcgggtgt
     6421 tattatagta caaaatttgt aattttgtta caaaaaaatc agacatcgaa aagaaatttg
     6481 atttaaatcg cagaatcttt cgattgttgt aacggaatag gggaaacctg cccgattttt
     6541 ggactaggtc aaataaattg taaaaatggg aatagacaaa aagtgtagcg gtgtgtagta
     6601 ttactactca ttatgccgcc tgcctttgtt tccgatattg cgatgcagcg tgatggggaa
     6661 aacaggtttc aaacggcatc gaaaaattct ttcctgacga gataatacga tgagtacaca
     6721 aacaaatttt gatttggtgt tgttcggtgc gaccggcgat ttggcgatgc gcaagctgtt
     6781 gccctgtctg taccaggcgc atgttgccgg tctgcttcat cccgaagggc gtattttggg
     6841 ggtcagccgc agcgagttgg ataccgaagg ttttctggcg aaggtggaaa ccagttccaa
     6901 aatccatgtg aaagaaaatt tttccgatga ggcttgggca tcgtttgtcg aacgtttcgc
     6961 atatctcaaa gtcgatgtta cgcagccgga cgattttgcc gctttgggcg atttggtcaa
     7021 agcgcgcaaa gaaaccgaca atgtcgtcat ctatctttcc accgcgccga aattcttcgc
     7081 gcaaacatgt gaaaaccttg ccaaaatcgg tttgaacgcc gacaacgtgc gtgtggtttt
     7141 ggaaaaaccg ttgggtacgg atttggcttc ttcccagcaa atcaataccg atgtcgcgcg
     7201 ttatttcaaa gaagggcaaa tttaccgcat cgaccactac cttggcaaag aaagcctgca
     7261 aaacctgctt gcgctgcgtt ttgccaatgt aatgttcgag ccgctgtgga acaataaata
     7321 catcgaaagc gtgcagttga ccatcgccga gcagttgggc gtggaagaac gcggcgagtt
     7381 ttacgacatt accggcgcgt tgcgcgatat ggtgcaaaat cacttgatgc aaatgctgtg
     7441 catgactgcg atggaagccc ccgccagctt ggatgccgac gcggtgcgcg atgaaaaagt
     7501 caaagtcatc aagtcattga agccgctgac cgtcgaatct gtcaatgaaa atgtcgtgcg
     7561 cggacaatat accgccgcca aaggcatgaa cggctatctt gaagaaatca acgttccgca
     7621 agacagcttt accgaaacct acgtcgccat taaagccgaa atcgaaaacg aacgctggaa
     7681 gggcgttccc ttctacctgc gtaccggcaa gcgcatggcg ggcaaagtgg cggaaatcgt
     7741 tttgaacttc aaagatttga acagccatat ttttgaaggc agccgcaccg cgcccaaccg
     7801 gctcgttatc gagttgcaac catatgaatc cgtgcgcctc tatacgcaga tgaaaacccc
     7861 gggggcagga aataaggtcg aaaccgtgcc gctggcaacc gatttgggca aagcattgga
     7921 aagccgccgc gcggaagctt acgagcgcct gctgctggat gtgattaacg gcaaactcgc
     7981 tttattcaac cgccgcgacg aactcgaagc cgcgtgggaa tatgtgatgc cgattttgga
     8041 aaactggaca aataacacca cgccgccgca cggctacggc gcacactcg
//
LOCUS       DAWHSK010000070         8017 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.070, whole genome shotgun sequence.
ACCESSION   DAWHSK010000070 DAWHSK010000000
VERSION     DAWHSK010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8017
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.070"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(127..1044)
                     /locus_tag="WIC03_000975"
     CDS             complement(127..1044)
                     /locus_tag="WIC03_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HEZ3883497.1"
                     /translation="MNVSVLNFGNTPVSFRQDGFLNATAIASHFGKLPKDYLKSEQTQ
                     QYISALAENLSVRRKILTEANQIVIVKRGGSEQGTWLHPKLAIHFARWLNPKFAVWCD
                     EQIEILLNGKISDGIKTVTPKPTRALPDGLTGEQIEAVKKLHNALTKSAPKEAQARIA
                     ITLWSAVKSKFGCSYKEVPAEQFPEVLSVMGRVAVENGVLYGEVLDREPLPAPQPALP
                     ISGNALYDLAVAVRYGAWAIQMGRDVSLPLKQLGCKQAVTMWTVWAETRSRLKAAANA
                     LEALNAHADAEHAAKIRPMLPEIRNLSSV"
     gene            complement(1913..2269)
                     /locus_tag="WIC03_000976"
     CDS             complement(1913..2269)
                     /locus_tag="WIC03_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4760 domain-containing protein"
                     /protein_id="HEZ3883498.1"
                     /translation="MAERNNAALQEAITIVNGLAKTDGCILATYTSDTPDKKKDREAI
                     LTVLNQREFVCAGVLGGALHEKMYKDFEYSMLLRDWDNLSSFIFEIRRIRSAPTAFQE
                     FEAVARKWKKKPLKTK"
     gene            complement(2817..3197)
                     /locus_tag="WIC03_000977"
     CDS             complement(2817..3197)
                     /locus_tag="WIC03_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEZ3883499.1"
                     /translation="MPLKFQPRERSVIMCDFRGYEEPEMVKKRPVVVIARNRHNGKLV
                     TVVPLSSTEPVPLADYHHKMSGNPLPDKPHIQCWAKCDMTATVGLARLDRYKPKGRDR
                     CIPIISEEDFQAIKTAVAKAFKLY"
     gene            complement(3354..4550)
                     /locus_tag="WIC03_000978"
     CDS             complement(3354..4550)
                     /locus_tag="WIC03_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HEZ3883500.1"
                     /translation="MAKIITPLSANQVKNAKPRDKLYKLSDGGGLALWVYPTGGRSWK
                     LSFVQDGRQQTISLGRYPDFSLADAREWREEVRRKRAHGENVVNKKVRADFAFEKVTR
                     DWFVRWSKGRSEKYAGQVMRNFERWVFPAIGNLDIRQVRTADVVGCLRVMEARGIVDT
                     LRKTKNSLKMVFAFAVGSGMMEINPVAQIGSGVFERAKTGNMAALSPSELPRLIDFLE
                     QRNEFAVYAGRVRIHPVTRLCIYWLLLTMTRIQEAALMEWSELDGEVWRIPAERKKER
                     RGHDVPLSRAMQWVLDQARALNVNGRFVFESVNFQGHINKESPRVAMQRAGLDTTAHG
                     LRSLARTYLREVLKVDNDVAEKLLAHSLGTRTQTAYNRSELWEERKDALERWGNDVLR
                     LAKNGK"
     gene            complement(4741..4830)
                     /locus_tag="WIC03_000979"
     tRNA            complement(4741..4830)
                     /locus_tag="WIC03_000979"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4794..4796),aa:Ser,seq:cga)
     gene            complement(4934..5024)
                     /locus_tag="WIC03_000980"
     tRNA            complement(4934..5024)
                     /locus_tag="WIC03_000980"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4988..4990),aa:Ser,seq:tga)
     gene            complement(5107..5886)
                     /gene="yaaA"
                     /locus_tag="WIC03_000981"
     CDS             complement(5107..5886)
                     /gene="yaaA"
                     /locus_tag="WIC03_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEZ3883501.1"
                     /translation="MFFVLSPAKNLNEKDPAPVSEFTQPDLLAESDILMQQLRELAPQ
                     QIAELMHVSDKIALLNAQRNAEWHTPFTPENAKQAVFMFNGDVYEGMDANTLDIGQIR
                     YLQNHVRLLSGLYGLLRPLDLIQPYRLEMGTAFANLRGKNLYEFWGDIITNLLNDTLA
                     QAGSNTLVNLASQEYFKSVNTKKLRARLITPIFKDEKNGKYKIISFYAKRARGLMVRY
                     AVEHHITDPEMLKNFNYEGYAFNDAASNESEWVFMRSEQIK"
     gene            complement(6149..7336)
                     /gene="dapC"
                     /locus_tag="WIC03_000982"
     CDS             complement(6149..7336)
                     /gene="dapC"
                     /locus_tag="WIC03_000982"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HEZ3883502.1"
                     /translation="MNTLLKQLKPYPFARLHEAMQGISAPEGMEAVPLHIGEPKHPTP
                     KVITDALTASLHELEKYPLTAGLPELCQACANWLKRRYDGLTLNSDTEILPVLGSREA
                     LFSFVQTVLNPVSDGLKPVIVSPNPFYQIYEGATLLGGGEIHFANCPAPSFNPDWRSI
                     SEEVWKRTKLVFVCSPNNPSGSVLDLDGWKEVFDLQDKYGFIIASDECYSEIYFDGNK
                     PLGCLQAAAQLGRSRQKLLMFTSLSKRSNVPGLRSGFVAGDAELLKNFLLYRTYHGSA
                     MSIPVQRASIAAWNDEQHVIDNRRLYQEKFEWVIPILQQVFDVKLPDASFYIWLKVPD
                     GDDLAFARNLWQKAAIQVLPGRFLARDTEQGNPGEGYVRIALVTDVATCVKAAETIVS
                     LYR"
     gene            complement(7413..7865)
                     /gene="dut"
                     /locus_tag="WIC03_000983"
     CDS             complement(7413..7865)
                     /gene="dut"
                     /locus_tag="WIC03_000983"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEZ3883503.1"
                     /translation="MNIEVEMKVLDERMADFIPAYATEGSAGLDLRACLDEEVVLQPG
                     ETFLVPTGLAIYLANPAYAAVLLPRSGLGHKHGIVLGNLVGLIDSDYQGELKVSLWNR
                     SSEPFTVKPFERIAQMVIVPIVQARFKRVEEFVGSSRGEGGFGSTGLH"
BASE COUNT         2016 a         2074 c         1874 g         2053 t
ORIGIN      
        1 aaaaccccac tcacatcccc cgtatgggac acccgcgaag ttttggaagc cctggggtta
       61 cagcaataaa aaaacgcccg attagggcgt tttcacattc agacggcttt tgtattccct
      121 actgcatcaa accgacgaca ggttgcggat ttcgggcagc atcgggcgga tttttgccgc
      181 gtgttccgcg tcggcgtgtg cgtttaaggc ttcgagggcg tttgcggcgg ctttgaggcg
      241 gctgcgtgtt tccgcccaga ccgtccacat cgttaccgcc tgtttgcagc cgagctgctt
      301 cagcggcagg gaaacgtctc tgcccatttg gatggcccac gcgccgtatc tgacggcaac
      361 ggcgaggtcg tacagggcgt tgccgctgat gggcagggca ggttgcggtg cgggcaatgg
      421 ttcgcggtcg aggacttcgc cgtacagcac gccgttttca actgccacgc ggcccatcac
      481 gcttaaaact tcggggaact gttcggcagg tacttctttg tagctgcatc cgaacttgct
      541 tttgacggca gaccaaaggg taatggcgat acgcgcctgc gcttctttgg gtgcggattt
      601 ggtcagggcg ttgtgcagtt ttttgacggc ttcgatttgt tcgccggtca agccgtccgg
      661 aagggcgcgg gtgggtttgg gggtaactgt ttttatgccg tctgaaattt tgccgttaag
      721 taaaatttca atctgctcat cgcaccaaac cgcaaatttc ggattaagcc aacgggcaaa
      781 gtgaatagcg agtttgggat gcagccatgt gccttgctca ctgccaccac gcttcacgat
      841 aactatttga tttgcttccg ttaggatttt tctcctaacg cttaaattct cagcaagtgc
      901 agagatatat tgttgagttt gttcactttt taggtagtct ttaggtaact tgccaaagtg
      961 agatgcaatg gcggttgcat ttaaaaaacc gtcttgacgg aaagatacag gggtgttacc
     1021 aaaattgaga acagatacgt tcatgatagt ttcctagttt agtttcgaaa gacccaaatg
     1081 ggtggtcggg aggttcgaaa acctactaga aaaggccggc gttattcccc ttgcgggtat
     1141 tgtattccgc accctcccga ccataggaaa ctagttcgga tatatcgaaa caaacaacca
     1201 aacaagaaaa cttatggacg taaaaaattc acgctgacgg ggtaaatgcc gttctagtag
     1261 aggttttcga cgcctcgtgt ttcgtaatat atccaatctt tctattttct gcaagcataa
     1321 aaaagccgcc atgtggcgga tagggtcgcg gtcttcaaca gcatgtagga tttaggaatg
     1381 cccaaattgg gcgcaggggt gttgaaaaca cgcaaacagg cgcgccgcca gccttacggg
     1441 taggcgcacc cccacatagg agtaaatcga tttgatacgt atagacgtaa aaaattccac
     1501 tctatcgtgt tggatatacg ctgtttgtca ggtgttttca agcaccgtgg gaaatattat
     1561 atagagttat ggaaatgtgt caagagaatt agcccaaatg ggcggacgcg cggttaagaa
     1621 cccacatagg agggcggact tattcccctt tcgggtcttg tattcgtcgc ccacgcggtc
     1681 atagaaactt cctgctatcg aaacaaacaa caaggaaaga aaactataga catgaaaaaa
     1741 tcacattgac ggagtgattg ccgctgtagt gtggtttctg acgccacgaa caggaatata
     1801 aaacaaaacc ccctgcacat gcaaggggtt tcccaaaaaa ccgtaggcgg caaactgaaa
     1861 ggccgtctga atttcagacg gcctatgttg cggcggatgt taagctatta agctatttgg
     1921 ttttcagagg ctttttcttc cattttcggg ctacggcttc aaattcttga aaggccgtcg
     1981 gtgcgctcct gatacggcgt atttcaaaaa taaagctgct taggttgtcc cagtcacgta
     2041 acagcatgga gtattcgaaa tctttataca ttttctcgtg cagtgctccg cctaatacgc
     2101 ccgcacagac aaattcgcgc tggtttaaaa ctgtcagtat ggcttcacgg tctttcttct
     2161 tgtccggggt atccgatgta taggtggcga gtatgcatcc gtctgttttt gccagcccgt
     2221 ttactatggt tatggcttct tgaagggcgg cattgttacg ttcggccatg atcagcgcgc
     2281 tttttggcgt tttctgtatt cctcatgatg ccgtaagcgg caacaaaaac gccgataaca
     2341 gtcaatatcg gcgttgctat ctgtattagg ttgtcagtca ttgccgtccc agccgtctga
     2401 aaggcggaaa tcacggcttt ccgaataaat atgtcccatt gtcatcccct taagctgatg
     2461 ttgaaaataa atccttgcgt cggaatatac ggcattgctt gactattttc aagattctgt
     2521 acctcgtgaa ccggactata caaaaaaacc gccttcgtgt aaagcggttc ttaaatcgcc
     2581 cgttaaggcg gacacaacga tagccaaact ggaacaggag gcggcaaagg cgggtttgtc
     2641 caaaacagcc ttgattgaaa ggctgattgg ggatttgtag gcgtttgcag atacgacaag
     2701 gggcgcattc cgcgccccat cacacgcacg gacggcttgt catcgcctgt cgcccgtgcg
     2761 cggctgccca tatttcagaa cagtcttgca agccccttta agggaacggt tttattctag
     2821 tacagtttga atgccttggc aacggctgtt ttaatcgcct gaaaatcctc ttcactgatt
     2881 attggaatgc agcggtcgcg ccctttgggt ttgtatcggt ctaatcgtgc caatccgact
     2941 gttgccgtca tgtcgcattt tgcccaacat tggatgtgcg gcttgtccgg taaggggttt
     3001 ccactcattt tgtggtggta gtccgccaaa gggacaggtt ctgtgctgct taagggtacg
     3061 accgttacca gtttgccgtt gtgcctgttt cgcgctatga cgacgacagg gcgtttcttg
     3121 accatttccg gttcttcata accgcgaaag tcgcacatga taaccgaacg ttcccttggt
     3181 tggaatttta aaggcattag ccgctcctga cgatgacagg ctgctattat agcagttctt
     3241 acacaaaaaa ccgccttcgt gtaaagcggt tgcaaaaaag ccttccaata aaaatgcgga
     3301 aacggttttt tatttgcgtt tccgcataat acaaacacac acacaaaaaa aaatcatttt
     3361 ccgtttttgg caagcctcaa aacatcattc ccccaccgtt ccagcgcatc cttacgctcc
     3421 tcccaaagct ccgagcggtt gtaagccgtt tgcgtcctcg tccccaacga atgggcaagc
     3481 agcttttccg ccacatcatt atccaccttc agaacctcgc gcaaataagt acgcgcaagc
     3541 gagcgcaaac cgtgcgccgt cgtatccagc cccgcccgct gcatcgccac gcgcggactt
     3601 tccttattga tatgcccttg aaaattcaca ctttcaaaca caaaccgccc gttcacattc
     3661 aacgcccgcg cctgatccaa cacccactgc atcgcccgcg acagcggcac atcatgcccc
     3721 cgccgctcct ttttccgttc ggcggggata cgccaaacct ccccgtccaa ctccgaccac
     3781 tccatcaacg ccgcctcctg aatccgcgtc attgtcaaca gcagccaata gatacaaagc
     3841 cgcgttacag gatggatacg caccctgccc gcataaaccg caaattcatt gcgctgctcc
     3901 aaaaaatcaa tcaggcgcgg caattcagac ggactcaacg ctgccatatt ccccgttttc
     3961 gcccgttcaa acacacccga accgatttgc gcgacagggt tgatttccat cattcccgaa
     4021 ccgaccgcaa acgcaaacac catcttcaga ctgtttttcg ttttgcgcaa cgtatcaacg
     4081 ataccgcgcg cctccatcac acggaggcag ccgaccacat ccgccgtcct gacctggcga
     4141 atatcaagat tgccgatagc cggaaaaacc caccgttcaa aattccgcat aacctgtccg
     4201 gcatactttt cagaccgccc cttcgaccaa cgcacaaacc aatcacgcgt caccttctca
     4261 aaagcaaaat ccgcccgcac cttcttattg acgacatttt ccccgtgcgc ccgttttcgg
     4321 cgcacctcct cccgccattc ccgcgcatcg gccagcgaaa aatcaggata ccgccccagc
     4381 gaaattgtct gctgccttcc atcctgcaca aacgacagct tccaactccg cccgcccgtc
     4441 gggtagaccc acaaagccag cccgcccccg tctgacaact tatacagctt atcgcgcggc
     4501 ttcgcatttt taacctgatt tgccgacagc ggcgtaatga ttttcgccat tatggtaatt
     4561 tcccacttat ccaaaaatta ccatatacat taccataaaa aaatgcaata agaagatacc
     4621 gcaagattgc aaatgattta ctttgacgcg ttttgatagg gcaaaccgat gatttttata
     4681 tggtaattga cacaacttga acaaaaaaga accgccctga atcagggcgg ttttgttttc
     4741 tggcggaaac ggtgggattc gaacccacgg aggatttgca ccctcagcgg atttcgagtc
     4801 cgctgcattc agcctctctg ccacgtttcc gaaagatgtt tcaacctaaa aggaaacacg
     4861 atgaagtaaa accaaataaa aatgccgatg ctttatgcta cttcattttt atttggtttt
     4921 tgacctcaag gattggcgga agcggtgaga ttcgaactca cggagggcta tcaaccctcg
     4981 acggttttca agaccgttgc attaaaccgc tctgccacgc ttccgtcttc ttgaagagta
     5041 ggaataataa tgaagtttat acattttgcc aagcactttt ttcggaaaat acttaattta
     5101 ttgttttcat tttatttgtt ccgaacgcat aaaaacccat tcgctttcat ttgaagccgc
     5161 gtcattgaat gcgtagcctt cgtaattaaa atttttcagc atttcagggt cggtaatatg
     5221 atgttctacc gcatagcgca ccattaatcc gcgcgcgcgc ttggcataga aactgatgat
     5281 tttatattta ccgttttttt cgtctttaaa tatcggggta atcagccgcg cccgaagttt
     5341 tttcgtgttg acggacttga aatattcctg tgaggcaagg ttgacaagcg tattgctgcc
     5401 tgcttgggca agcgtgtcat ttaaaaggtt ggtaatgatg tcgccccaaa actcatacaa
     5461 attcttgccg cgcaaattgg caaatgccgt ccccatttcc aaacggtagg gctgtatcag
     5521 gtctaacggg cgaagaagac cgtacagacc ggacagcagg cggacatggt tttgcagata
     5581 gcgtatctgt ccaatatcca atgtgtttgc atccatacct tcgtaaacat cgccgttgaa
     5641 cataaagacc gcctgtttgg cgttttccgg cgtaaacggc gtgtgccatt ctgcattgcg
     5701 ctgcgcgttt aagagggcaa ttttgtcgga aacgtgcatc agttcggcaa tctgttgcgg
     5761 cgcaagctcg cgcaactgct gcattagaat gtcggactct gccagcaggt cgggttgggt
     5821 aaactcgctg acaggggcag ggtctttttc attaaggttc ttcgcagggg aaaggacaaa
     5881 aaacataatc tgctcggtat tgtgtcaaac acggggattt taagtttaaa cgtgtttgcg
     5941 ggcaagccga ttaaaagtga agtatagtgg attaaattta aaccagtaca gcgttgcctc
     6001 gccttgccgt actatctgta ctgtcttcgg cttcgtcgcc ttgtcctgat ttttgttaat
     6061 ccactataaa taccgttata gcaaattcaa ccgtaaaata ttgaaaaatg ccgtctgaac
     6121 aaaagttcag acggcatttt tttattcttt accgatatag ggaaacaatg gtttccgcag
     6181 ctttgacaca agttgcgaca tcggtgacca aagcgatacg cacataacct tccccgggat
     6241 tgccctgttc ggtatcccgt gccaaaaagc gtccgggcaa tacttggata gcggcttttt
     6301 gccataaatt gcgtgcaaat gccaaatcgt cgccatcggg gactttcaac cagatgtaaa
     6361 acgaggcatc cggtaatttg acgtcaaata cctgttgcag gataggaata acccattcaa
     6421 acttttcctg atacaggcgg cggttgtcaa taacgtgctg ttcgtcattc caggcggcaa
     6481 tactggcacg ctgcacggga atgctcattg cgctgccgtg ataagttcgg taaagcagaa
     6541 agtttttaag cagttcggca tcgccggcga caaaaccgga acgcaggccc ggaacattgg
     6601 accgcttgga caaactggtg aacataagca gtttttgcct gcttcgaccc aactgtgcag
     6661 cggcttgcag gcagcccaaa ggtttgttgc cgtcgaaata gatttcggaa tagcattcat
     6721 ccgaggcaat aatgaaacca tatttgtctt gcaaatcaaa aacttctttc cagccatcca
     6781 aatccagcac gctgccactg gggttgttgg gcgagcagac gaacaccagt ttggtgcgtt
     6841 tccaaacctc ttcggaaata ctgcgccaat cggggttgaa agacggcgcg gggcaattag
     6901 caaaatggat ttcaccgccg cccaaaagtg tcgcaccttc ataaatctga taaaagggat
     6961 tcgggctgac gattacgggt ttgaggccgt ctgaaacagg gttcaacacg gtttggacaa
     7021 aagaaaacaa cgcctcccta cttcccaaaa ccggcagaat ttcggtgtcg gaattcagcg
     7081 tcaacccatc ataacggcgt tttaaccagt tcgcacacgc ctgacacagt tcaggcaaac
     7141 cggccgtcag cggatatttt tccaactcgt gcaatgaggc ggtcagcgca tccgtaatga
     7201 ctttcggtgt cggatgtttc ggttcgccga tatgcagtgg gacggcttcc atgccttcag
     7261 gcgcggaaat gccctgcatc gcctcgtgca gtcgggcaaa gggatagggt ttgagctgct
     7321 tgagtaaggt attcatgatt ttctctggtt atcggtatat cggaagatat gccgtctgaa
     7381 cctgacggca cccatcagac ggcattttat attcaatgca aacccgtact gccgaagccg
     7441 ccctcacccc ggctgcttcc gacaaattcc tcgacacgtt tgaaacgcgc ctgcacgatt
     7501 ggcacgataa ccatctgcgc gatacgctca aacggtttga cagtaaacgg ctcgctgctt
     7561 ctgttccata acgaaacctt caattcccct tgataatcgg agtcaatcaa gccgaccaaa
     7621 ttgcccaaaa caatgccgtg tttatgcccc aagccggaac ggggcagcaa aacggcggca
     7681 tatgcgggat tcgccaaata aattgccaaa cctgtcggta caagaaacgt ttcacccggc
     7741 tgcaagacga cttcctcatc caaacaggcg cgcaaatcta aacctgcaga accctccgtt
     7801 gcataggcag ggataaaatc cgccatcctc tcgtccaata ctttcatttc tacttcaata
     7861 ttcatcgtct tgctcctgca tacggccgat ttcaaagatg ggagattata cacaagccgt
     7921 gtttacgctg aaacacggca aatcggtttt gttttgaaat ccgttgccag tgtataatgc
     7981 ccgcccttcc cgttatgaag acgaacatta tgcacac
//
LOCUS       DAWHSK010000071         7954 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.071, whole genome shotgun sequence.
ACCESSION   DAWHSK010000071 DAWHSK010000000
VERSION     DAWHSK010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7954
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.071"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(1..192)
                     /locus_tag="WIC03_000984"
                     /pseudo
     CDS             complement(1..192)
                     /locus_tag="WIC03_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611451.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            248..817
                     /locus_tag="WIC03_000985"
                     /pseudo
     CDS             248..817
                     /locus_tag="WIC03_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691695.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
     gene            1049..1549
                     /locus_tag="WIC03_000986"
     CDS             1049..1549
                     /locus_tag="WIC03_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003798052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883504.1"
                     /translation="MVFVNPLYYGREAGWFVWLAAAMCGLWLLRALTAKLPQQRYAAL
                     FITLFFAAVLVFRRHDSMYWYPVLVNLMMLAVFGGSLFAGQTVIERLARLQQPDLPEK
                     AVRYTRRVTQVWCVFFIANGTLAALLARLGRYDWWAVYTGVIAYVLMGMPFAGEWLYR
                     KLVLKV"
     gene            1556..2071
                     /locus_tag="WIC03_000987"
     CDS             1556..2071
                     /locus_tag="WIC03_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2199 domain-containing protein"
                     /protein_id="HEZ3883505.1"
                     /translation="MYTCTSCGEKHEDMPAIGFTAPDPYNQLSEEERIAYQAECDSDF
                     CIIRYPDQTDRFIRAVLPIPIIGHEETLEYGVWVSVSEKSFNDYQSHFHDNPENAVYF
                     GMICNRLPPYETDTFGLHCNVVTQPDGQRPLLQLHQGSEHPLVRDFYHGIEYAEAQAR
                     IGAVSESDCSG"
     gene            2091..3569
                     /gene="ubiD"
                     /locus_tag="WIC03_000988"
     CDS             2091..3569
                     /gene="ubiD"
                     /locus_tag="WIC03_000988"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HEZ3883506.1"
                     /translation="MKYKDLRDFIAMLEQQGKLKRIAHPVSPHLEMTEIADRVLRAEG
                     PALLFEHPVKPDGTRYDYPVLANLFGTPERVAMGMGADSVSKLREIGQTLAYLKEPEP
                     PKGIKDAFSKLPLLKDIWSMAPNVVKNAPCQEIVWEGEDVDLYQLPIQHCWPEDVAPL
                     VTWGLTVTRGPHKKRQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEFRKLNPDTPYP
                     VAVVLGCDPATILGAVTPVPDTLSEYQFAGLLRGSRTELVKCIGNDLQVPARAEIVLE
                     GVIHPNETALEGPYGDHTGYYNEQDYFPVFTVERITMRENPIYHSTYTGKPPDEPAVL
                     GVALNEVFVPLLQKQFPEITDFYLPPEGCSYRMAVVSMKKQYAGHAKRVMMGCWSFLR
                     QFMYTKFIIVVDDDVNVRDWKEVIWAVTTRMDPVRDTVLVENTPIDYLDFASPVSGLG
                     GKMGLDATNKWPGETDREWGRVIKKDPAVTAKIDGIWEELGL"
     gene            complement(3633..5753)
                     /locus_tag="WIC03_000989"
     CDS             complement(3633..5753)
                     /locus_tag="WIC03_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEZ3883507.1"
                     /translation="MDLLSVFHKYRLKYAVAVLTILLLAAIGLHASVYRIFTPENIRS
                     RLQQSIAHTHRKISFDADIQRRLLPRPTVILKNLTITEPGGDRTAVSIKETKIGLSWK
                     NLWSDRIQVEKWVVSGAELALTRDGKGVWNIQDLIDNPKRKASVNRIIIENSTVRLNF
                     LQERFTLKEVRLNLQSPVSSGQQFESSGTLTGENLSASWKSKGLFISDGINPPEISPF
                     HFEAETALNGHGITVSTTGNPTIRFNAGGADAANLGLRADTAFHNLHLTAQIPTLALR
                     NNSIKIETVNGTFTATADDGNFQWGGSFKLDKANLHSGIGNIGNAEISGNVKTPRLQT
                     NFSLGSPLVWSRNNGLEAPRLHVSTLQDTVNRLPQPRFISRLDGSMSIKNLHQWHAEF
                     NGSFDRQTVAAKFRYTHEDAPHLEAAVALQKLNLTPYLDDVRQQNGKIFPDTLGRLSG
                     DIEAHLKIGNVQLPGLQLDDMETYLHADKGHIALSRFKSGLYGGHTEGGISIANTRPA
                     TYRLQQNASNIQIQPLLQDLFGFHSFSGNGDAVIDLTASGETRKELIRSLQGSLSLNI
                     SNGAWHGINMDNILKNGISGKTADNAVPSTPFHRFTLNSEISDGISRHIDTELFSDSL
                     YVTSNGYTNLDTQELSEDVLIHNAVHPKNKPIPLKITGTVDKPSITVDYGRLTGGINS
                     RKEKQKILEDTLLEQWQWLKPKEP"
     gene            6028..6879
                     /gene="folP"
                     /locus_tag="WIC03_000990"
     CDS             6028..6879
                     /gene="folP"
                     /locus_tag="WIC03_000990"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEZ3883508.1"
                     /translation="MVGCVWQAGRFEIGLDKPKIMGIVNLTPDSLSDGGAYSQNAKTA
                     LAHAERLLKEGADILDIGGESTRPGAGYVSPEEEWARVEPVLAEVAGWGVPVSLDTRR
                     TVVMEKALALGGIDIINDVAALTDEGAVELLARQTDTGICLMHMQGLPENMQINPKYQ
                     DVVGEVTRYLKARAAECIAAGITPQRITLDPGFGFGKTLQHNIALMRHLPELMAETGL
                     PLLIGVSRKSMIGELTGEADAAARVHGSVAAALASVARGAQIVRVHDVKATADALKAW
                     EALGINL"
     gene            7009..>7954
                     /gene="glmM"
                     /locus_tag="WIC03_000991"
     CDS             7009..>7954
                     /gene="glmM"
                     /locus_tag="WIC03_000991"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEZ3883509.1"
                     /translation="MAKKYFGTDGVRGEVGQFPITPDFVLKLGYAAGQVLVQHDTDQK
                     PTVLIGKDTRISGYMLEAALVAGFTAAGVNVVQTGPLPTPGVAYLTRALRLSAGVMIS
                     ASHNAYSDNGIKFFAEGGVKLSDEIELEIEAKIDEEMKTQPSARLGRARRISGADDRY
                     IEFCKSTFPSHSDLRGLKLVIDTANGAAYSVAPKVFHELGAQVISIGDEPDGYNINEK
                     CGATHTKTLQAAVLQNEADYGIALDGDGDRLMMVDKNGQVYDGDSLIYVIAKARAREG
                     INIGGVVGTVMTNMAMEIALKEQGVDFCRAKVGDRYVLE"
BASE COUNT         1740 a         1941 c         2392 g         1881 t
ORIGIN      
        1 ctcaaaaacc tcatcggcag ccaaggccaa atgatggttg ataaccatgc ggattttacg
       61 gtagaacagt gctgccgaat tgggatggat acccaaaata tcggcggcag aacgggcggt
      121 aacttcgagt acaaaaaaac ggagcagttc tttctgtact tttttctttc atttgcagtg
      181 cgttatcttc atatttcgag ggtaacatat ctgctaatct agtacagccc ctaaaattta
      241 atccactata gtttatgcct cgcacgacgg ggaaatggcg cgcagtttcg atttgtggtt
      301 ggaactgttg aaatcgcaaa tcgtgtcgcc gacttcgttc ggtttgtcgg tgcacaatgc
      361 aacggcgggg taatggtcga tattgaggcg ggatatgagc gagcagacgg cattggccgt
      421 gtgtgccgac ggtgtggaaa cggcattggc ggaagcggca tcactgttgg aagagggatg
      481 cgggtcggtt ttggttttgg cggcggacga tccgctgccg gaaggatatg ccgtatcggc
      541 aacgcgcgcg ccgtttgctt atgctttggc gatggttctg accaaaggga cgcgttacag
      601 cctgacttta tctgcttcgg acgatatgcc gtctgaagcc ggtatgctgc ccgaggcata
      661 ttggagcggt ttggaatggg tgcgctttct gctgaacggc agtcgggaat gccgccgggt
      721 atatcgcaat cgtgaatggc tgtggcagcc cgcaagctgc aggctgaaga tttccgcaat
      781 gtccgcaccg tcaacgacgt ggtacaggcg gtattgaaga ttcaggcggg ctaatcgggt
      841 atttctaatt atttttcaga cggcatgttt ttatcggatg ccgtctgaaa acatttgtgc
      901 ggattcaaaa tgaaacccgg ttttttcgga catatttttc tgaccgtatt aagcattgcc
      961 tatcccgctt tgtggtatag tggattaaca aaaatcagga caaggcgacg aagccgcaga
     1021 cagtacggca aggcgaggca acgctgtact ggtttttgtt aatccactat attacggcag
     1081 ggaggcgggc tggtttgtct ggctggcggc ggcgatgtgc gggttgtggc tgctgcgcgc
     1141 cctgactgcc aaactgcccc aacagcgtta tgcggctttg tttattacac tgttttttgc
     1201 cgcagtattg gtgttcagac ggcatgattc gatgtattgg tatcccgtcc tcgtcaatct
     1261 gatgatgttg gcggtgttcg gcggcagcct gtttgccgga cagacggtca tagaaaggct
     1321 cgcgcggctg caacagcccg acctgcctga aaaagccgtg cgctataccc gccgggtaac
     1381 gcaggtttgg tgcgtattct ttattgccaa cgggacgctt gccgctttgt tggcacggtt
     1441 ggggcgatat gattggtggg cggtttatac cggcgtgatt gcctatgtat tgatggggat
     1501 gccgtttgcc ggcgaatggc tttaccgcaa actcgttttg aaagtctgaa accttatgta
     1561 cacttgcact tcctgcggcg aaaaacatga agatatgccc gcaatcggct ttaccgcccc
     1621 cgatccctat aatcaactca gtgaagaaga acgcattgct tatcaagcag aatgcgattc
     1681 tgatttctgc atcatccgct atcccgacca aaccgaccgc tttatccgcg cggtgctgcc
     1741 gattccgatt atcgggcatg aggaaacgct ggaatatggc gtgtgggtgt ccgtcagcga
     1801 aaagagtttt aacgactatc aaagccactt tcacgacaat ccggaaaacg ctgtttattt
     1861 cggcatgatc tgcaatcggc tgccccctta tgaaaccgac accttcggtt tgcattgcaa
     1921 tgtcgttacc caacccgatg gtcaacgtcc tttgttgcaa ctccatcaag gcagcgaaca
     1981 tccgctggta cgcgactttt atcacggcat agaatatgcc gaagcgcagg caaggatagg
     2041 ggcggtatcc gaatcggatt gctcaggata aaaggacaaa aaggatgaat atgaaataca
     2101 aagacctgcg cgacttcatc gccatgctcg agcagcaggg caaactcaaa cgcatcgcgc
     2161 accccgtttc cccgcatttg gaaatgaccg aaatcgccga ccgcgtgctg cgcgccgaag
     2221 ggccggcgtt gttgtttgaa cacccagtta agcccgacgg tacgcgctat gattatcccg
     2281 tgttggcaaa cctgttcggc acgcccgaac gtgtggcgat gggcatgggc gcggacagcg
     2341 tgtccaagct gcgcgaaatc gggcagacgc tggcgtattt gaaagaaccc gaaccgccca
     2401 aaggcattaa agacgcgttt tccaaactgc cgctcttgaa agacatttgg agcatggcgc
     2461 cgaacgtggt gaaaaatgcg ccgtgtcagg aaatcgtatg ggaaggcgaa gacgttgatt
     2521 tgtatcaact tccgattcag cattgctggc cggaagacgt tgcgccgctg gtaacgtggg
     2581 gcttgaccgt cacgcgcggg ccgcacaaaa aacgccaaaa tctcggcatt taccgccaac
     2641 aattaatcgg caaaaacaag ctgattatgc gctggctgtc gcatcgcggc ggcgcgttgg
     2701 attatcagga attccgcaaa ctcaatcccg atacgccgta tcccgtcgcc gtcgtgctcg
     2761 gctgcgaccc tgccaccatt ttgggcgcgg taacgcccgt tcccgatacc ttgagcgaat
     2821 accagtttgc cggactgctg cgcggttcgc ggacggagct ggtgaaatgt atcggcaacg
     2881 atttgcaagt gcctgcacgc gcagaaatcg tgctggaagg tgtgattcat ccgaacgaaa
     2941 ccgcgttgga aggcccgtac ggcgaccaca cgggctatta caacgagcag gattatttcc
     3001 ctgtgtttac ggtcgaacgc atcaccatgc gcgaaaaccc gatttaccat tcgacctaca
     3061 caggcaaacc gcccgatgag cctgccgttt tgggcgtggc gttgaacgaa gtgttcgtac
     3121 cgctcttgca aaagcagttt cccgaaatca ccgatttcta cctgccgccc gaaggctgct
     3181 cctaccgcat ggcggtggtg agcatgaaaa aacagtacgc cggacacgcc aaacgcgtga
     3241 tgatgggctg ctggtcgttc ctgcgccagt ttatgtatac caaattcatt atcgtggtgg
     3301 atgacgatgt gaacgtgcgc gactggaaag aagtcatctg ggcggtcacc acgcgcatgg
     3361 accccgtgcg cgacactgtt ttggtagaaa acacgcccat cgattatctc gacttcgcca
     3421 gccccgtcag cggactcggc ggcaaaatgg gtttggatgc gaccaacaaa tggccgggag
     3481 aaaccgaccg cgaatgggga cgcgtcatca aaaaagaccc tgcggttacg gctaagattg
     3541 atgggatttg ggaggaattg gggttgtagg ttttacggtc tgccgtctga agcgcggggg
     3601 cgtttcagac ggcatttttg tacgcagggt atttacggtt ctttaggttt gagccactgc
     3661 cattgttcca gcagggtgtc ttcgaggatt ttctgtttct ctttgcgcga attgatgccg
     3721 ccggtcagcc tgccgtaatc gacggtaatg gacggtttgt ccaccgtacc ggtgattttc
     3781 aggggaatcg gtttgttttt cggatggacg gcgttgtgga taaggacatc ttcagacaat
     3841 tcctgcgtat ccagattggt atagccgttg ctggtaacat agaggctgtc ggagaagagt
     3901 tcggtatcga tgtggcggct gatgccgtct gaaatttcgc tgttgagcgt gaatcggtgg
     3961 aagggggtgc tgggtacggc attgtcggca gttttgcccg aaatgccgtt tttcaggata
     4021 ttgtccatgt tgataccgtg ccatgcgccg ttggaaatat tcagcgacag gctgccctga
     4081 agcgagcgga taagctcttt tcgggtttcg ccactcgcgg tcaggtcgat gaccgcatcg
     4141 ccgttgccgc tgaagctgtg gaagccgaac aggtcttgca gcagaggttg gatttggatg
     4201 ttgcttgcat tctgttgcag gcggtaagtg gcgggacggg tgttggcgat gctgatgcca
     4261 ccttcggtat ggccgccgta aagccctgac ttgaaacggc tgagcgcaat atggcctttg
     4321 tcggcgtgga ggtaggtttc catatcgtcc agttgcaagc cgggaagttg gacattgccg
     4381 attttaaggt gggcttcgat atctccggac agcctgccga gggtgtcggg aaatattttg
     4441 ccgttttgtt gccgcacgtc gtcaagatag ggggtcaggt tcaatttttg cagtgcgacg
     4501 gcggcttcca ggtgcggtgc gtcttcatgc gtgtatctga atttcgcggc aacggtttgg
     4561 cggtcgaacg aaccgttgaa ttcggcatgc cactgatgaa ggttttttat ggacatcgaa
     4621 ccgtcgagcc ggctgatgaa acggggttgc ggcaggcggt tgacggtatc ctgaagggtt
     4681 gatacatgca ggcgcggcgc ctccagaccg ttgttccggc tccaaaccaa tggcgagccg
     4741 agggaaaagt tggtttgaag gcggggtgtt ttgacgttgc cggagatttc ggcgttgccg
     4801 atgttgccga tgccggagtg caggttggcc ttatcgagtt tgaacgatcc gccccattgg
     4861 aaattcccgt cgtcggcggt agcggtaaat gtcccgttga cggtttcaat tttaatgctg
     4921 ttgttcctga gtgccagcgt agggatttgg gcggtcaggt ggaggttgtg gaaggcggtg
     4981 tcggcacgca ggccgaggtt ggccgcatcc gctccgccgg cgttaaaacg gatagtgggg
     5041 ttgccggtgg tggaaacggt aatgccatgc ccgtttaggg cggtctcggc ttcgaaatgg
     5101 aacggtgaga tttcgggcgg attgatgccg tctgaaatga aaagcccttt gcttttccat
     5161 gaggcagaca ggttttcgcc ggtcagtgtg cccgaacttt caaactgttg tcccgatgaa
     5221 acgggggatt gcaggttgag gcggacttcc ttcagggtaa accgctcttg caggaagttg
     5281 aggcggacgg tgctgttttc gataatgatg cggtttaccg aggctttgcg ttttgggttg
     5341 tcgatcaggt cttggatgtt ccaaacgcct ttcccgtcgc gcgtcagggc aagttccgca
     5401 cccgaaacca cccatttttc aacctgtatc cgatccgacc acagattttt ccagctcaat
     5461 ccgattttgg tttctttgat ggaaacggca gtccggtcac cgccgggttc ggtaatggtc
     5521 aggtttttca ggatgacggt cggccggggc agaagcctgc gctgtatatc cgcatcaaac
     5581 gagattttcc ggtgcgtatg ggcaatgctt tgttggaggc ggcttcggat gttttcaggt
     5641 gtgaagatgc gatatacgga agcgtgcagc ccgattgccg ccaaaagcag tatcgtcagc
     5701 acggctaccg catatttcag acggtatttg tggaagaccg ataataaatc catagcctga
     5761 gaagtctgta aaacacgcca cagtataaca cggcttgtag aaatcctatt ttgtgcgggc
     5821 ggattatctt gtataaaggg gtaataccca taagcaggca taccgccgga tcaggtatcg
     5881 agggtgttgc cgcgtttgca ggggcagtgt atgtttcata cggcggcgga ttctgcaaga
     5941 tacttttttg aaataccgat accgtctgaa acggacaagt tttgttcaga cggtatataa
     6001 tttgcttttt attccggagg cacggatatg gtcggatgcg tttggcaggc aggacggttt
     6061 gaaatcggtt tggacaaacc gaaaatcatg ggtatcgtga atctcacgcc cgattctctt
     6121 tccgacggcg gcgcgtattc gcaaaacgcc aaaaccgctt tggcgcatgc cgaacggctt
     6181 ttgaaagagg gtgcggacat tctcgacatc ggcggcgaat cgacgcggcc gggtgcgggt
     6241 tatgtttcgc ctgaagaaga atgggcgcgg gttgagcctg tattggcgga agtggctggg
     6301 tggggcgttc ccgtcagttt ggacacgcgc cgcacggtgg ttatggaaaa agcattggca
     6361 ctcggcggca tcgatattat taacgatgtg gcggcgttaa ccgacgaagg cgcggtcgaa
     6421 ttgctggcgc gccagacgga cacgggcatc tgcctgatgc acatgcaggg tttgcctgaa
     6481 aacatgcaga ttaatccgaa atatcaggat gtcgtcggtg aagtgacgcg gtatttgaaa
     6541 gcgcgggcgg cggaatgcat cgcggcaggc atcaccccac aacgcatcac acttgacccc
     6601 ggttttggct tcggcaaaac cctgcaacac aatatcgcac tgatgcggca tttacccgaa
     6661 ttgatggcag aaaccggttt gccgctgctt atcggcgtgt cgcgcaagag catgataggc
     6721 gagctgaccg gcgaggcaga cgcggcggcg cgcgtacacg gcagcgtggc ggcggcattg
     6781 gcgtccgtag cgcgcggcgc gcaaatcgtg cgggtgcatg atgtgaaggc gacggcggat
     6841 gctttgaagg cgtgggaagc gttgggcatc aatctgtaaa cagcggttca catacttcat
     6901 gccgtctgaa aagggcgggg cggcttcaga cggcatcaac ggtgcagaca gcaaggcaca
     6961 aactgactgt ataatctcgg acaacggttc acatgctcgg agcaaatgat ggcaaaaaaa
     7021 tatttcggca cggacggcgt gcgcggcgaa gtcgggcagt ttccgattac gcccgatttt
     7081 gtattgaaac tcggctatgc ggcggggcag gttttggttc agcacgacac cgaccagaaa
     7141 ccgaccgttt taatcggcaa agacacgcgt atttccggtt atatgctgga agccgctttg
     7201 gtggcaggtt ttacggcggc gggtgtgaat gtggtacaga ccggaccgct gcccacgccc
     7261 ggcgtggctt atctgacccg tgcgctgcgt ttgtccgccg gtgtgatgat ttccgcgtcg
     7321 cacaatgcct attccgacaa cggcatcaaa ttctttgccg agggtggtgt gaagctttcc
     7381 gatgaaatcg agttggaaat cgaagccaaa atcgacgaag aaatgaaaac ccagccttct
     7441 gcacgcctcg gtcgcgcacg ccgcatcagc ggtgcggacg accgctatat cgaattttgc
     7501 aaatccacct tccccagcca ttccgacttg cgcgggctga agctggttat cgataccgcc
     7561 aacggcgcgg cttacagcgt cgcacccaaa gtgtttcacg aactgggcgc gcaggtcatc
     7621 agcatcggcg acgaacccga cggctacaat atcaatgaaa aatgcggcgc gacccatacc
     7681 aaaaccctcc aggccgccgt gttgcaaaac gaggcagact acggcatcgc gctggacggc
     7741 gacggcgacc gcctgatgat ggtcgataaa aacgggcagg tttacgacgg cgacagcctg
     7801 atttacgtca ttgctaaagc ccgagcccga gaaggtatca acatcggcgg cgtggtcggc
     7861 acggtcatga ccaatatggc gatggaaatc gccctgaaag agcagggcgt cgatttctgc
     7921 cgcgccaaag tcggcgaccg ctatgtgttg gaac
//
LOCUS       DAWHSK010000072         7863 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.072, whole genome shotgun sequence.
ACCESSION   DAWHSK010000072 DAWHSK010000000
VERSION     DAWHSK010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7863
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.072"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..1337)
                     /locus_tag="WIC03_000992"
     CDS             complement(<1..1337)
                     /locus_tag="WIC03_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase/DUF3482 domain-containing protein"
                     /protein_id="HEZ3883510.1"
                     /translation="MNKQPLSLAVVGHTNTGKTSLLRTLLRDSGFGEVKNAPSTTRHV
                     EEAAISDGADMLVFLYDTPGLEDAGGVLEWLETHTDTRSDGIERLQQFLGSHGAHHDF
                     NQEAKVLRQVLQSDMAMYVIDAREPVLDKYRDELTILSWCAKPVMPVFNFTGGQPPES
                     WTTMLARRNLHVFAGFDTVAFDFEGELRLWENLATMLPERSTLDRLTAMRRREWQRLD
                     GEARREIADFLIDAAAFRQEVDENEDTATVLQTMQAEIRQLERQMQQRLFALYRFYHS
                     EIDGGDWMPQAFRQDPFDSELLKQYGIRTGTGAATGALIGLGLDIATLGGSLGLGTAI
                     GGFLGGILSNTRTISDKLAGRQTLHTDPETLTLLAARALDLLHVLQTRGHAAQSDIEL
                     HSRKAPWDAARLPPELNKARSHWKWSSLNTHRPETSRAERAGYVEKLQIRLSG"
     gene            complement(1399..2748)
                     /locus_tag="WIC03_000993"
     CDS             complement(1399..2748)
                     /locus_tag="WIC03_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2868 domain-containing protein"
                     /protein_id="HEZ3883511.1"
                     /translation="MLNPSRKLVELVRILEEGGFIFSGDPVQATEALRRVDGSTEEKI
                     IRRAEMIDRNRMLRDTLERVRVGSFWLWVVAASMMFTAGFSGTYLLMDNQGLNFFLIL
                     AGVLGMNTLMLAVWLAMLFLRVKVGRFFSSPATWFRGKDPVNQAVLQLYADEWRQPSV
                     RWKIGATSHSLWLCTLLGMLVSVLLLLLVRQYTFNWESTLLGDSSSVRLVEMLAWLPA
                     KLGFPVPDARAVIEGRLNGNIADARAWSGLLVGSIACYGILPRLLAWVVCKILLKTSE
                     NGLDLEKPYYQAVIRRWQNKITDADTRREIVSAVSPKIILNDAPKWAVMLETEWQDGE
                     WFEGRLAQEWLDKGVAANREQVAALETELKQKPAQLLIGVRAQTVPDRGVLRQIVRLS
                     EAAQGGAVVQLLAEQGLSDDLSEKLEHWRNALTECGAAWLEPDRAAQEGRLKTNDRT"
     gene            2891..3517
                     /locus_tag="WIC03_000994"
     CDS             2891..3517
                     /locus_tag="WIC03_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEZ3883512.1"
                     /translation="MYTGERFNTYSHLSGLILAAAGLVLMLLKTIGHGDGYRIFSVSV
                     YGISLLLLYLSSSLYHGIAAGKLKSILKKTDHCMIYVLIAGSYTPFALVSLRNGPGWT
                     VFSLSWLLAAAGIAQELTIGRKSEKRLLSIVIYVVMGWMVLAVMKSLTASLPSAGLAW
                     LAAGGMLYSVGIYWFVNDEKIRHGHGIWHLFVLGGSITQFVSVYGYVI"
     gene            complement(3581..4999)
                     /locus_tag="WIC03_000995"
     CDS             complement(3581..4999)
                     /locus_tag="WIC03_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEZ3883513.1"
                     /translation="MENILSVLVGTVNRFLWDYLLIYALLGIGLFFTLYLGAPQITKL
                     GAGFKSVFGGLFAKGDKDDKSLSQFQALAVAISAQIGTGNVAGVATAITAGGPGAIFW
                     MWVSAVLGMSTIFAEALLAQKYRVVSHGKYIGGPAFYITHGLTPKIGRGAARFLSGFF
                     SIALIIALGFIGNATQANSIASAVTIAFDVPSLAVGIVLAVLAGMVVIGGVNRIANIA
                     QFVVPFMAVVYILCAVVILFEFSDHIVPMFNHIFTAAFNPEAVLGGAAGIGMREAIRF
                     GVARGLFSNEAGMGSTPHAHATADVKHPVQQGMAAFVGVFIDTILVCTATALIILLTD
                     ANLSGEQGAAVTQFAFNKAFPGFGSQLLAVCLTFFALTTIIGWYYFGESNIRFLFRGR
                     HLGIYRALVLLAIVLGTLGKVDLVWSLSDMFNGFMVIPNLIALFLLRKEIRAIYDDYL
                     AQKKAGRDLSYPYEFHEFHDKG"
     gene            5326..6054
                     /locus_tag="WIC03_000996"
     CDS             5326..6054
                     /locus_tag="WIC03_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEZ3883514.1"
                     /translation="MAGHSKWANIQHKKARQDAKRGKIFTRLIKEITVAARMGGGDPG
                     ANPRLRLALEKAAENNMPKDNVQRAIDKGTGNLEGVEYIELRYEGYGIGGAALMVDCL
                     TDNKTRTVADVRHAFTKNGGNLGTDGCVAFNFVHQGYLVFEPGVDEDALMEAALEAGA
                     EDVVTNDDGSIEVITAPNDWAGVKSALEAAGYKSVDGDVTMRAQNETELSGDDAVKMQ
                     KLIDALEDLDDVQDVYTSAVLNLD"
     gene            complement(6113..6601)
                     /locus_tag="WIC03_000997"
     CDS             complement(6113..6601)
                     /locus_tag="WIC03_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HEZ3883515.1"
                     /translation="MTPLFRKAVWLLFAVSVCAFAGSLAAQYVLGMEPCVLCISQRLC
                     VLATALCTAIVLMCRPRRRAGGLFGAVFISIPAVTGISVAAYQLWLQSLPPGTAPSCG
                     APWTFRLKGWSLFDWFEPVVRGFGNCAEPDYLLGVALPVWSAAYFLAVVLTVWWAWAR
                     AK"
     gene            complement(7359..7823)
                     /locus_tag="WIC03_000998"
     CDS             complement(7359..7823)
                     /locus_tag="WIC03_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEZ3883516.1"
                     /translation="MKELDKIDFRILKILQQNARIPMTELAEKVGLSTTPVTERVRRL
                     EREHYISGYHAHLNPHLLGKPLLVFVELKLQSKSGNIFEDFKKEVLKIPQIMECHLVS
                     GEYDYLIKVRLPDMSAYRDMLGNILLQLPAASESRSYVVMEEVKENPVLDLD"
BASE COUNT         2023 a         2123 c         1979 g         1738 t
ORIGIN      
        1 tttcccgaca ggcggatttg aagtttctcc acatatccgg cgcgttcggc gcggctggtt
       61 tcggggcggt gcgtattgag cgacgaccat ttccaatgac tgcgggcttt gttgagttcg
      121 ggcgggagtc tggcggcgtc ccacggggct ttgcggctgt gcagctcaat atccgactgt
      181 gccgcgtgtc cgcgcgtttg caggacgtgg agcagatcga gggcgcgggc ggcgagcagg
      241 gtcagggttt cggggtcggt gtgcaaggtt tggcggccgg cgagtttgtc ggaaatggtg
      301 cgggtattgg acaggatgcc gcccaaaaag ccgccgattg ccgtacccaa tccgagcgag
      361 ccgccgagtg tggcgatgtc cagccccaag ccgatgagcg cgccggttgc cgcgcccgtg
      421 ccggtgcgga tgccgtattg tttgagcaat tcgctgtcga acgggtcttg gcggaaggct
      481 tgcggcatcc agtcgccgcc gtcgatttcg ctgtggtaga aacggtagag ggcaaacagc
      541 cgctgctgca tctgccgttc gagttggcgt atttccgcct gcatggtttg cagcacggtg
      601 gcggtatcct cgttttcgtc cacttcctgc ctgaaggcgg cggcatcaat taaaaagtcg
      661 gcgatttcgc ggcgcgcttc gccgtccagc cgctgccatt cgcgccggcg catggctgtc
      721 aggcggtcaa gtgtgctgcg ttcgggcaac atggtggcga ggttttccca caggcgtagt
      781 tcgccttcaa aatcaaaggc gacggtgtcg aaccctgcga aaacgtgcag gtttctcctc
      841 gccagcatgg ttgtccacga ttcgggaggc tgtccgccgg taaagttgaa cacgggcata
      901 accggtttgg cacaccatga aaggatggtc aattcatccc gatatttgtc gaggacgggt
      961 tcgcgcgcgt cgatgacgta cattgccata tcgctttgca agacttgccg taagactttg
     1021 gcttcctgat tgaaatcatg gtgcgcaccg tggctgccga gaaactgttg cagccgctcg
     1081 atgccgtctg aacgcgtatc cgtatgggtt tccagccatt ccaaaacacc gcccgcgtct
     1141 tcgagtccgg gcgtgtcata caggaaaacc agcatgtccg cgccgtcgct gatggcggct
     1201 tcttcgacat ggcgcgtagt ggacggggca tttttgactt cgccgaagcc gctgtcgcgc
     1261 aatagggtgc gcaggagcga ggttttgccg gtattggtat gcccgacgac ggcgagggaa
     1321 aggggttgtt tgttcatgat gtttttgaag aatggatttt cagacgacct tttaaggatg
     1381 gcggctttat ggggcgtttc aagtgcggtc gttggttttc agacggcctt cctgcgccgc
     1441 cctgtcaggc tcaagccacg ccgcgccgca ttcggtcagc gcgttacgcc aatgttccag
     1501 cttttccgaa aggtcgtctg aaagcccctg ttccgccaaa agctgcacca ccgcgccgcc
     1561 ctgcgccgct tccgaaagtc ggacgatctg ccgcaacacg ccgcggtcgg gcacagtttg
     1621 ggcgcgcacg ccgataagca gttgcgccgg tttctgcttc agctctgtct ccagcgcggc
     1681 aacctgttcc cgattggcgg caacgccctt atccagccat tcctgcgcca gcctgccctc
     1741 gaaccattcg ccgtcctgcc actcggtctc cagcatgacc gcccatttcg gtgcatcgtt
     1801 caagatgatt ttcggtgaaa cggcggacac gatttcccga cgcgtatccg catcggtgat
     1861 tttgttctgc cagcggcgga tgaccgcctg ataatagggc ttttccaaat ccaagccgtt
     1921 ctcgcttgtt ttcaaaagga ttttacacac tacccaagcc agcaggcgcg gcaggatgcc
     1981 gtagcaggcg atactgccga ccagcagccc cgaccaagcc cgcgcatcgg caatattgcc
     2041 gttcagacga ccttcgatga ccgctcgcgc atcaggcacg ggaaaaccca gtttcgcagg
     2101 cagccatgcc aacatttcca ccagccgtac cgaagacgaa tcgcccaaca gcgtgctttc
     2161 ccagttgaac gtatattgcc gcaccaaaag cagcaacaat accgacacca gcattccgag
     2221 cagcgtgcag agccacaggc tgtgcgacgt tgcgcctatt ttccaacgta ccgaaggttg
     2281 ccgccactcg tccgcataca gctgcaacac cgcctgattg acagggtctt tgccccgaaa
     2341 ccacgtcgcc ggactgctga aaaaacgccc cactttcacg cgcaggaaca acattgccaa
     2401 ccatactgct aacatcagcg tattcatgcc caacacgccc gccaaaatca ggaagaaatt
     2461 cagcccctga ttgtccatca gaagataagt gcctgaaaat ccggcggtaa acatcatcga
     2521 tgccgccacc acccacaacc agaacgaccc cacacgcaca cgttccaacg tgtcccgcag
     2581 catacggttc ctgtcgatca tctccgcccg acggatgatt ttttcctccg tactgccgtc
     2641 cacgcggcgc aaagcctccg tcgcctgcac gggatcgccg ctgaaaataa agccgccttc
     2701 ttccaaaata cggaccagct caaccagttt tcgggatgga ttcaacataa aatgccgtct
     2761 gaaaataaaa aacagatttt aacacacgaa ttttcaagaa tattcacagt gtaggcaaag
     2821 agtaaatccc acacagaggc aaaagtatcg gcgtaaactg actgcctcta ctttcccgaa
     2881 agattgtgcg atgtatacag gcgaacgctt caatacttac agccatttga gcggtttgat
     2941 tctggcggcg gcaggtttgg tgctgatgct gctgaaaacc ataggacacg gggacggcta
     3001 ccgtatcttc agcgtatcgg tttacggcat cagccttctt ctgctctatt tgagttcctc
     3061 gctgtaccac ggaattgcag ccggaaaact gaaaagcatt ttgaaaaaaa ccgaccactg
     3121 catgatttat gtcctaattg ccggaagcta cacaccgttt gcactggttt ctttgagaaa
     3181 cgggccgggc tggacggtat tttcactgtc ctggctgctg gcggctgcag gaatcgcaca
     3241 agaactcacc atcggacgga aaagcgaaaa acgtctgctg tctattgtga tttatgtcgt
     3301 catgggttgg atggtcttgg cggtaatgaa atccctgaca gcctcactcc cgtcggcagg
     3361 actggcttgg ctggcggcag gcggtatgct gtacagcgtc ggcatttact ggtttgtaaa
     3421 cgatgaaaaa atccgacacg ggcacggaat ctggcatctg ttcgtattgg gcggcagcat
     3481 cacccaattt gtcagtgtgt acggttatgt aatctgaatg ccgtctgaaa aacaaaacct
     3541 cccgatcctg aagattggga ggttttctgt ttgccggaca tcagcccttg tcgtggaact
     3601 cgtggaattc atacggataa gacaaatccc gacccgcttt tttctgtgcc aaataatcat
     3661 cataaatggc acggatttcc ttacgcaaca aaaacagggc tatcaagttc gggataacca
     3721 taaagccgtt gaacatatcg gacaggctcc aaaccaaatc gactttgccg agcgtaccta
     3781 aaacgatggc aagcagaacc aatgcgcgat agatgcccaa gtgtcttccc ctgaaaagaa
     3841 aacggatgtt ggactcaccg aaataatacc agccgataat ggtcgtcaag gcgaagaagg
     3901 tcagacacac ggcaagcaat tgcgaaccga agcccggaaa tgccttgtta aaggcaaatt
     3961 gagtaaccgc cgcgccctgt tcgcccgaaa ggttggcatc ggtcagcagg ataatcagcg
     4021 cagttgccgt acataccaaa atcgtatcga taaacacacc gacaaatgcc gccatacctt
     4081 gctgcacagg atgcttcaca tccgcagtcg cgtgggcgtg cggagtcgaa cccatccccg
     4141 cctcattgga aaacagaccg cgcgccacgc cgaaacgtat cgcttcgcgc ataccgatac
     4201 ccgccgcacc gcccaaaacg gcttcgggat tgaaggcggc ggtaaagatg tggttgaaca
     4261 tcggcacaat atggtcggaa aattcaaaca ggataacgac ggcgcacaaa atataaacaa
     4321 ccgccataaa cggcacgaca aattgggcaa tattggcaat acggttcacg ccgccaatca
     4381 caaccatgcc cgcaaggacg gcaagcacaa taccgactgc caaagaaggc acatcaaatg
     4441 caatggtaac ggcagaagca atggagtttg cctgtgtcgc attaccgata aagcccaatg
     4501 cgataatcaa cgcaatggaa aagaaaccgg acaaaaaacg cgccgctccc ctgccgattt
     4561 tcggagtcag accgtgggtg atgtagaacg ccggcccgcc gatgtatttg ccgtggctga
     4621 cgacgcggta tttctgcgcc agcagtgcct ccgcaaaaat cgtggacatc cccaaaacgg
     4681 cagaaaccca catccaaaaa atcgcgcccg gcccgcctgc ggtgatggcg gtcgccacgc
     4741 cggcgacgtt gcccgtaccg atttgcgcgg atatggcaac cgccaacgcc tgaaactgcg
     4801 ataaagactt gtcgtcttta tcgcctttgg caaacaagcc gccgaatacg gatttgaatc
     4861 ccgcgcccag cttggtaatc tgcggcgcac caagatacag cgtaaaaaac aggccgatac
     4921 ccaaaagcgc gtaaatcagc aggtagtccc aaaggaaccg attgactgta cccaccagaa
     4981 cagacaatat attttccata aaataaacct tatcttacaa ttaaaatgac tgccttccaa
     5041 aagacattcc aataaggaaa cacggcgagc agaccgtatt tgccgcaaca gatgccttaa
     5101 attgtcaaca atcggggaga agctgcgcca tcataccgta aaatatcgtt aattaaaaca
     5161 tttctttatt tttaagcgga aagcggagga aatcgctttc agacagcata gacaacggca
     5221 cggcataaaa caggatattt tgggtacttg caacttatgt taaaatgccg accgtaaaaa
     5281 atctgacaaa aacagattaa ttatttgaaa taagaaagga aatttatggc aggccatagc
     5341 aagtgggcga atatccagca taaaaaagcc cgtcaggatg ccaaacgcgg caaaatcttc
     5401 acccgtttaa tcaaagaaat caccgttgcg gcgcgtatgg gcggcggcga ccccggtgcc
     5461 aacccgcgcc tgcgcctggc tttggaaaaa gcagccgaaa acaatatgcc caaagacaat
     5521 gtgcaacgcg ccatcgataa aggtacgggc aacttggaag gcgtggaata catcgagttg
     5581 cgctacgaag gctacggcat cggcggcgcg gctttgatgg tggactgcct gaccgacaac
     5641 aaaacccgca ccgttgcgga cgtacgccac gcgtttacca aaaacggcgg caacttgggt
     5701 accgacggct gcgtggcgtt caacttcgtg catcagggct atttggtatt cgaacccggc
     5761 gttgacgaag acgcgctgat ggaagcggct ttggaagccg gtgcggaaga cgtggttacc
     5821 aacgacgacg gttccatcga agtcattacc gcgccaaacg attgggcggg cgtaaaatcc
     5881 gctttggagg cggcaggtta caaatccgtt gacggcgacg ttacgatgcg cgcccaaaac
     5941 gaaaccgaac tctccggcga cgatgccgtc aaaatgcaaa aactgattga cgcgctggaa
     6001 gacttggacg acgtgcaaga cgtttacact tccgccgtat tgaatctgga ctgatacgca
     6061 gcacagcaga catacaataa aatgccgtct gaacctttca gacggcattg atttatttag
     6121 cccttgccca cgcccaccaa accgtcagga caaccgccag aaaatacgcc gcgctccaaa
     6181 caggcaaagc aacccctaac agataatccg gttcggcaca atttccgaac ccgcgcacga
     6241 caggctcgaa ccaatcaaac aaagaccagc ccttcaagcg gaacgtccac ggcgcaccgc
     6301 acgaaggagc cgtacccggc ggcagcgact gcaaccacaa ctgatatgcc gcaacagaaa
     6361 tacccgtaac ggccggaatg ctgataaaga cagcaccgaa caaaccgccc gcccttcttc
     6421 ttggtctgca catcaggaca attgccgtac acaatgcggt tgccaaaacg cataaccgct
     6481 gactgataca caaaacgcaa ggctccatac ccaaaacata ctgtgccgcc aaagaaccgg
     6541 caaatgcaca gaccgaaacg gcaaacagca gccaaacggc ttttctaaat aacggggtca
     6601 ttttctcaac acaccaatca aaataccgat atgccgattt tgctggatat atcccgagat
     6661 ggcaagggac gaatcgacac cggcgcaggt gcggacgatt tcggttttcc cgatatacac
     6721 aagacctgac aagggacaaa acggcgattt gcccgcacaa tgcccgcaaa aaaatacatc
     6781 cggaggattt gaatttcggc aaatttagcg aatcaaaagt ttatttcaat gaaattatat
     6841 gatttttttg aataagcgga ttgacaggtt tttgaaggaa tttgttaccg gatagccatc
     6901 gggcaagttt tttgcaaaat ttgaatcggt cgggtaaatt ttcaaaaaat aattgacagc
     6961 ggataagaaa cggcggataa ttctcgccgt cgagttgctt gatgcagctt gatttttctc
     7021 ctctatttct cctttgtaga cttggcacac attcaaccgg atgtgtgcat ttttttatct
     7081 ccgctttttt tgaaatttaa ttacttttat tgtttgaaat tccattcttt aagaaattta
     7141 acaagaaaaa cgcttgcttt ttgaccggaa aagctgcata attcgttccg ttagctggtt
     7201 cgagtagtca gttaatagtt tctcctctat ttctcctttg tagacttggc acacattcta
     7261 ttgaatgtgt gcattttttt atctgaagca acaagcctct gtgcgtgatg ttgttatgtt
     7321 tcatttaggt gtcaaaccgc atatccggtc tgaaatattc aatccaaatc caaaaccgga
     7381 ttttctttga cctcctccat cacaacataa ctcctactct ccgaagcggc aggcagttgc
     7441 aataggatat tgcctagcat atcccgatag gcagacatat cgggcaaacg tactttaatc
     7501 agatagtcgt attcgcccga caccaagtgg cattccataa tttgcggaat tttcagcact
     7561 tcttttttga aatcttcgaa aatattgccc gatttggatt gcagcttcag ctcgacaaaa
     7621 accaatagtg gtttgcccaa cagatgggga ttgaggtggg cgtgatagcc ggaaatataa
     7681 tgttcccgct ccaaacggcg caccctctct gtaacgggcg tggtggacaa gcctaccttc
     7741 tcggcaagct ccgtcatcgg gatgcgggca ttctgttgaa ggatcttaag aatgcggaaa
     7801 tcgattttat ctagttcttt catttggatt gccttgtatt tattattgat tttaacaaat
     7861 aga
//
LOCUS       DAWHSK010000073         7819 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.073, whole genome shotgun sequence.
ACCESSION   DAWHSK010000073 DAWHSK010000000
VERSION     DAWHSK010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7819
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.073"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(26..2605)
                     /gene="clpB"
                     /locus_tag="WIC03_000999"
     CDS             complement(26..2605)
                     /gene="clpB"
                     /locus_tag="WIC03_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEZ3883517.1"
                     /translation="MRYDKLTAKFQQALAEAQSLALAADGSYLEAGFVLKALLDDQNS
                     GAAALLAHAGVNVPQVKQRLQQHLNSLPKVSGQGGDIMPSRELQAVLNLMDKAATKRG
                     DAYIASELFLLALVQQNDATGKILKEAGATEQNINAAIDAVRGGQNVNDANAEDQRDA
                     LKKYTLDLTQRARDGKLDPVIGRDDEIRRAIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVNGEVPESLRNKRLLVLDLAALIAGAKYRGEFEERLKGVLNDLAKDDGNTLIF
                     IDEIHTLVGAGKTDGAMDAGNMLKPALARGELHCIGATTLDEYRQYIEKDAALERRFQ
                     KVLVGEPSVEDTIAILRGLQERYEIHHGIDITDPAIVAAAELSDRYITDRFLPDKAID
                     LIDEAASRVKMEKETKPEAMDKIDRRLIQLRMEKAHVEKEKDDASKKRLELIDEEING
                     LQKEYADLDEIWKAEKAISDGAANIKKQIDEVKIKIEQAKRQGDLALASKLMYEDLEH
                     LEKQRAAAERADTDSTKPANKLLRNNVGAEEIAEVVSRMTGIPVSKMMEGERDKLLKM
                     EEVLHRRVVGQDEAVRAVSDAIRRSRSGLADPNKPYGSFLFLGPTGVGKTELCKALAG
                     FLFDSEDHLIRIDMSEYMEKHAVARLIGAPPGYVGYEEGGYLTEQVRRKPYSVILLDE
                     VEKAHPDVFNILLQVLDDGRLTDGQGRTVDFKNTVIVMTSNIGSQHIQQMGTQDYEAV
                     KEVVMEDVKEHFRPEMINRIDEVVVFHGLDQANIRSIAKIQLKGLEKRLEKQNLRLTV
                     SDAALDIIAKAGFDPIYGARPLKRAIQSEIENPLAKALLAGNYAPESEIRVEADGDRL
                     KFA"
     gene            2866..3876
                     /gene="trpS"
                     /locus_tag="WIC03_001000"
     CDS             2866..3876
                     /gene="trpS"
                     /locus_tag="WIC03_001000"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEZ3883518.1"
                     /translation="MSKKRVLTGVTTTGIPHLGNYVGAIRPAVRAAQNPDTESFLFLA
                     DYHGIIKCHEPEMIHQSTQAVAATWLACGLDPERTTFYRQSDIPEVMELNWILTCITA
                     KGLMNRAHAYKAAVQANAENNQEDPDFGVEMGLFSYPILMTADILMFNANEVPVGRDQ
                     IQHVEMARDIAGRFNHRFKEIFTLPEVKIDENVELLVGLDGRKMSKSYGNTIPLWEND
                     KKTQKSVNKIITNMKEPGEPKQPDESPLFEIYKAFSTPSETVEFTKMLADGLAWGEAK
                     KLLAAKINAELAEPRERYNELTADPSQIEEILQAGAQKARKEARELLDKVRDAVGIRP
                     LK"
     gene            3957..4502
                     /locus_tag="WIC03_001001"
     CDS             3957..4502
                     /locus_tag="WIC03_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883519.1"
                     /translation="MLKTSFAVLGGCLLLAACGKSENMAEQPQNAVQSAPKPVFKVKY
                     IDNTAIAGLALGQSSEGKTNDGKKQISYPIKGLPEQNVVWLTGKHTNDLEAVVGKCME
                     TDGKGAPSGWAANGVCHTLFAKLVGNIAEDGGKLTDYLISHSALQPYQAGKSGYAAVQ
                     NGRYVLEIDSEGAFYFRRRHY"
     gene            4821..5144
                     /locus_tag="WIC03_001002"
     CDS             4821..5144
                     /locus_tag="WIC03_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883520.1"
                     /translation="MKKLLIAAMMAAALAACSQEAKQEVKEAVQAVESDVKDTAASAA
                     ESAASAVEEAKDQVKDAAADAKASAEEAVTEAKEAVTEAAKDTLNKAADATQEAADKM
                     KDAAK"
     gene            complement(5353..6861)
                     /gene="ppx"
                     /locus_tag="WIC03_001003"
     CDS             complement(5353..6861)
                     /gene="ppx"
                     /locus_tag="WIC03_001003"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HEZ3883521.1"
                     /translation="MTTTPANVLASVDLGSNSFRLQICENNNGQLKVIDSFKQMVRFA
                     AGLDEQKNLSAASQEQALDCLAKFGERLRGFRPEQVRAVATNTFRVAKNIADFLPKAE
                     AALGFPIEIIAGREEARLIYTGVIHTLPPGGGKMLVIDIGGGSTEFVIGSTLNPDITE
                     SLPLGCVTYSLRFFQNKITAKDFQAAISAARNEIQRISKNMRREGWDFAVGTSGSAKS
                     IRDVLAAEMPQEADITYKGMRALSERIIEAGSVKKAKFENLKPERIEVFAGGLAVMMA
                     AFEEMKLDRMTVTEAALRDGVFYDLIGRGLNEDMRGQTVAEFQHRYHVSLNQAKRTAE
                     TAQTFMDSLCHAKNVTVQELALWQQYLGRAAALHEIGLDIAHTGYHKHSAYILENADM
                     PGFSRKEQTILAQLVIGHRGDMKKMSGIIGTNEMLWYAVLSLRLAALFCRSRQDLSFP
                     KNMQLRTDTESCGFILRIDREWLERHPLIADALEYESVQWQKINMPFKVEVV"
     gene            complement(6977..7709)
                     /locus_tag="WIC03_001004"
                     /pseudo
     CDS             complement(6977..7709)
                     /locus_tag="WIC03_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629272.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
BASE COUNT         1753 a         2040 c         2072 g         1954 t
ORIGIN      
        1 cggcatttcc aacagcagga acgaatcagg caaatttcag tctgtcgccg tcggcttcca
       61 ccctgatttc gctttcgggc gcatagtttc cggcaagcag ggcttttgcc agcgggtttt
      121 cgatttccga ctggatggcg cgtttgagcg gacgtgcgcc gtaaatcggg tcgaaaccgg
      181 ctttggcgat gatgtccagt gcggcatcgg aaacagtcag gcgcaggttt tgtttttcca
      241 aacgtttttc caaacctttg agctggattt tcgcaatgct gcggatgttt gcctgatcca
      301 aaccgtggaa cacgaccact tcgtcaatac ggttgatcat ttcaggacgg aaatgttctt
      361 tcacatcctc catcacaact tctttcaccg cttcgtagtc ctgcgtgccc atttgttgga
      421 tgtgctggct gccgatgttg gaagtcatga cgataacagt gtttttgaag tccacggtgc
      481 gaccttgccc gtcggtcagg cggccgtcat ccaatacttg caacaggata ttgaacacgt
      541 cgggatgggc tttttccact tcgtccaaca gaatcacgct gtacggtttg cggcggactt
      601 gttcggtcag gtagccgccc tcttcgtagc cgacatagcc cggaggcgcg ccgattaagc
      661 gggcaacggc gtgtttttcc atatattcgg acatatcgat acgaatcaga tgatcttcac
      721 tgtcaaacag gaagcctgcc aatgccttac acaattcggt cttacccacg ccggtcgggc
      781 ctaagaacag gaagctgccg taaggtttgt tcggatcggc aagaccggaa cggctgcggc
      841 ggatagcgtc ggacacggca cgcacggctt catcttgacc gaccacgcgg cggtgcaata
      901 cttcttccat cttcagcagt ttgtcgcgtt cgccttccat cattttggac acgggaatac
      961 cggtcatacg ggaaacaact tctgcgattt cctccgcgcc gacattatta cgcaagagtt
     1021 tgtttgccgg ttttgtgctg tccgtatctg cccgttcggc ggctgcacgc tgtttttcca
     1081 aatgctccaa atcttcatac atcaattttg aagccagtgc caaatcgcct tgccgttttg
     1141 cctgttcgat tttaattttg acttcgtcaa tttgtttctt aatattagca gcaccgtctg
     1201 aaattgcttt ttcggctttc cagatttcgt ctaaatcggc gtattctttt tgcagaccgt
     1261 tgatttcctc gtctatcagt tccaaacgtt ttttgctggc atcgtctttt tctttttcaa
     1321 cgtgtgcctt ttccatccga agctgaatca gacggcggtc gattttgtcc attgcttccg
     1381 gcttggtttc tttttccatc ttgacacggc tggcggcttc atcaatcaaa tcaatcgctt
     1441 tgtcgggcag gaagcggtcg gtgatgtagc ggtcgctcaa ctccgctgcg gcaacgatag
     1501 cagggtcggt aatatcgata ccgtggtgga tttcataacg ctcctgcaaa ccgcgcaaga
     1561 tggcgatggt gtcttccacg ctgggctcgc cgaccaatac tttttggaag cggcgttcga
     1621 gtgccgcgtc tttttcgatg tattggcggt attcgtccaa ggtggtcgcg ccgatacagt
     1681 gcagttcgcc ccgcgccaag gccggtttca gcatattgcc cgcgtccatc gcgccgtcgg
     1741 ttttgcccgc gccgaccaaa gtatggattt catcaatgaa aatcagagtg ttgccgtcgt
     1801 ctttcgccaa atcgttcaac acgcctttca agcgttcttc aaattcgcca cggtatttcg
     1861 cgccggcaat caatgccgcc aagtccagaa ccaaaaggcg tttgttgcgc agggattcgg
     1921 gtacttcgcc gttgacgatg cgttgcgcca agccttcgac aatggcagtt ttacccacgc
     1981 ccggttcgcc aatcagcacg gggttgtttt tggtgcggcg ttgcaatacc tgaattgcac
     2041 ggcggatttc gtcgtcacga ccgataacgg ggtcgagctt accgtcgcgg gcgcgttggg
     2101 tcaaatcgag cgtatatttt ttcagcgcat cgcgttggtc ttcggcattg gcatcgttca
     2161 cgttttgtcc tcctcgtacc gcatcgattg cggcattaat gttttgttcg gtcgcgccgg
     2221 cttctttcaa aattttgccg gttgcatcgt tctgctgcac caaggcgagc aggaaaagtt
     2281 cgctggcgat ataggcatcg ccgcgtttgg ttgctgcttt gtccatcagg ttcaacaccg
     2341 cctgcaattc gcggctgggc ataatgtcgc cgccctgtcc ggatactttc ggcaggctgt
     2401 ttaaatgctg ctgcaaacgc tgtttcacct gcggcacgtt cacgcccgca tgagccaaga
     2461 gcgcggcggc tccgctgttt tggtcgtcaa gcagggcttt taacacaaag cccgcttcca
     2521 gatagctgcc gtccgcagcc aacgccaaac tctgagcttc tgcaagggct tgttggaatt
     2581 tggcggttaa tttgtcgtat cgcatttttg tttccttttc aaaatgtccg ctgtcgaagc
     2641 ctatatgtgc ataattgtgg ataactcaag ttctgttttc tgtttttcta tatttaattc
     2701 gatatatcat tgaatttaaa gtatataaaa atgtataata atgtgtataa ctatatcttc
     2761 ttaatatgga aaagtctgtt gtcggctgga tgtaggtggc aaatcgggta taatcggcgc
     2821 atctttttcc ctttcagacg gcattgatgc cgcaaggaca tttttatgag caaaaaacga
     2881 gttctgaccg gcgtaaccac caccggcatc ccgcatctgg gcaactacgt cggcgccatc
     2941 cgccccgccg tccgcgcggc gcaaaaccct gataccgaat ccttcctctt cctcgccgat
     3001 taccacggta tcatcaaatg ccacgagccg gagatgattc accaatccac ccaagccgtt
     3061 gccgccactt ggcttgcctg cggactcgac cccgagcgca ccaccttcta ccgccaaagc
     3121 gacattcccg aagtgatgga attaaactgg attctgacct gcatcaccgc caagggtttg
     3181 atgaaccgcg cccacgccta caaagccgct gtgcaggcaa atgcagaaaa caatcaggaa
     3241 gatcccgatt tcggcgtgga aatgggtttg ttcagttatc cgattctgat gactgccgac
     3301 atcctcatgt tcaacgccaa cgaagtgccc gtcgggcgcg accaaatcca acacgtcgaa
     3361 atggcgcgcg acatcgccgg ccgcttcaac caccgcttta aagaaatctt taccctgccc
     3421 gaagtgaaaa tcgacgaaaa cgtcgaactc ttggtcggtt tggacggacg caaaatgtcc
     3481 aaatcctacg gcaacaccat tccgctttgg gaaaacgaca aaaaaaccca aaaatcggtc
     3541 aacaaaatca tcaccaatat gaaagagccg ggcgagccga aacagcccga cgaaagccca
     3601 ttgtttgaaa tctacaaagc cttctccacg ccgtctgaaa cggtggaatt tacgaaaatg
     3661 cttgccgacg gcttggcttg gggcgaggca aaaaaacttt tggcggcgaa aatcaacgcc
     3721 gaactcgccg aaccgcgcga acgctacaac gagctgaccg ccgacccttc gcaaatcgaa
     3781 gagattttac aggcaggcgc gcaaaaagcg cgtaaagaag cacgcgaatt gttggacaaa
     3841 gtacgcgatg cggtcggcat ccgcccgttg aaataaaccc aatgccgtct gaacatccat
     3901 tgtccgtgtt tcagacggca tttcagacgg cattttgaaa ccatcaggag tgtggaatgc
     3961 ttaaaacatc ttttgccgtg ttgggcggct gcctgctgct tgccgcctgc ggcaaatccg
     4021 aaaatatggc ggaacagccg caaaacgcgg tacaaagcgc gccgaaaccg gtttttaaag
     4081 tcaaatatat cgacaatacg gcgattgccg gtttggcttt gggacaaagc agcgaaggca
     4141 aaaccaacga cggcaaaaaa caaatcagtt atccgattaa aggcttgccg gaacaaaacg
     4201 tcgtctggct gaccggaaag cataccaacg acttggaagc cgtcgtcggc aaatgtatgg
     4261 aaaccgacgg aaagggcgca ccttcgggct gggcggcaaa cggcgtgtgc cataccttgt
     4321 ttgccaaact ggtgggcaat atcgccgaag acggcggcaa actgacggat tacctgattt
     4381 cgcattccgc cctgcaaccc tatcaggcag gcaaaagcgg ctatgccgcc gtgcagaacg
     4441 gacgctatgt gctggaaatc gacagcgagg gggcgtttta tttccgccgc cgccattatt
     4501 gaggtattcg gacatcccgg aatatatttg ggtttttcaa accctgcagg aaaaagtccg
     4561 caccgtcggg aaactcaaaa ggagggggat atgtgttaca attttccgaa ctgttttcat
     4621 aaaatagttt tcggacgtgt ttcaatatgg cattgatgcc gccttattgt tcgagaaaaa
     4681 acctttatat ttaaatataa tgggttttaa ctaaacggga aaccgttttc tctccggccg
     4741 atgggcaaaa tcagccgatt gatggaacac ggtccgattt ttaagcaaaa ccttaatcaa
     4801 caactcaaga aggataagac atgaaaaaat tattgattgc cgcaatgatg gcggctgcct
     4861 tggcagcttg ttcgcaagaa gccaaacagg aggttaagga agcggttcaa gccgttgagt
     4921 ccgatgttaa agacactgcg gcttctgccg ccgagtctgc cgcttctgcc gtcgaagaag
     4981 cgaaagacca agtcaaagat gctgcggctg atgcaaaggc aagtgccgag gaagctgtaa
     5041 ctgaagccaa agaagctgta actgaagcag ctaaagatac tttgaacaaa gctgccgacg
     5101 cgactcagga agcggcagac aaaatgaaag atgccgccaa ataatttgtt gccttggcaa
     5161 aaatgatggg atgccgcctg ccggcaaccc aaacgaaacc gcctgaagat tttcaggcgg
     5221 tttttgagtt gtatgcccct tgttttttac gcctgcatga ccgttccgag cagatgtgcc
     5281 gcgcatatcg ggatgttgcg gacaggcaaa tgccgtctgg acagggtttg gacggcattt
     5341 gttccgtaag gttcagacga cctcgacttt aaacggcata ttgatttttt gccattggac
     5401 gctttcatat tccaatgcgt cggcaatcag gggatggcgt tccagccatt ccctgtcaat
     5461 acgcaggatg aagccgcagc tttccgtatc cgtgcgcaac tgcatatttt tcgggaaaga
     5521 caggtcttgg cgcgaacggc agaacagggc agcaaggcgc agggacaaaa cggcatacca
     5581 caacatttcg ttggtgccga tgatgccgct catttttttc atatcgccgc gatgaccgat
     5641 gaccagttgg gcaagtatgg tctgttcttt gcgtgagaaa cccggcatat cggcgttttc
     5701 gaggatgtag gcggaatgct tgtgatagcc ggtgtgggcg atgtccaaac cgatttcgtg
     5761 cagcgcggcg gcgcgtccga gatactgttg ccacaaggca agctcttgaa ctgtaacgtt
     5821 tttagcgtgg cagaggctgt ccataaaggt ttgcgcggtc tcggcggtgc gtttcgcctg
     5881 attgaggctg acgtggtagc ggtgttggaa ctcggcaacc gtttgtccgc gcatatcttc
     5941 gtttaaaccg cgcccgatca aatcgtaaaa cacgccgtcg cgcagggcgg cttcggttac
     6001 ggtcatcctg tcgagtttca tttcctcaaa cgccgccatc atcacggcaa gtccgccggc
     6061 aaaaacttcg atgcgttccg gtttcaggtt ttcaaatttg gcttttttga ccgaaccggc
     6121 ttcgatgatg cgttcggaga gggcgcgcat acctttgtag gtaatgtccg cctcttgggg
     6181 catttcggcg gcaagcacgt cgcggatgga ttttgccgaa cccgatgtgc cgacggcgaa
     6241 atcccagcct tcgcgcctca tatttttgct gatgcgctgg atttcgttgc gggcggcgga
     6301 aatggcggct tggaagtctt tggcggtgat tttgttttgg aagaagcgca ggctgtaggt
     6361 aacgcagccc aagggcaggc tttcggtaat gtcgggattc agcgtcgaac cgatgacaaa
     6421 ttctgtcgaa ccgcccccga tgtcgataac cagcattttg ccgccgcccg gggggagggt
     6481 gtggatcacg ccggtataaa tcagccgtgc ctcttcgcgc ccggcgatga tttcgatggg
     6541 gaaacccagt gccgcttcgg ctttgggaag gaaatctgcg atgtttttgg cgacacggaa
     6601 tgtattggtt gccacggcgc gtacctgttc agggcggaag ccgcgcaggc gttcgccgaa
     6661 ttttgccaga cagtccaaag cctgttcttg ggaagcggca ctcaggtttt tctgttcgtc
     6721 cagtccggcg gcgaagcgca ccatctgttt gaacgaatcg atgactttca actgtccgtt
     6781 gttgttttcg caaatctgga ggcggaaact gttagaaccc aaatcgacgg aggcaaggac
     6841 gtttgcgggg gtggtggtca tggcggatac cggtggggga aaaacgcaat gttactctga
     6901 cggcgcgggc gttgacaata aatgatgcgg cggtttttga ttctgcccac ggatgttgcc
     6961 gacggcattt ttgcgcttat ttgaaatcct tttccacgct catgaaaatc tgcgtgtttt
     7021 tgcgtgtgta aaaacttttc atattgctgt cgattttcag atagcggaaa ttgagttgcg
     7081 gcgtaaagcc cttccaagag attttgtcgt gccacaacga caggtttgcc tgatattcgt
     7141 ggtctttgcg cgggaagcgg tacacaatgg tcccgggtgc gtcaaacatc ctgcgggtat
     7201 agcgcaggtt tgcccgcaga cccaagcccg ccgtcgaacg ttttgactgc gccgacacgc
     7261 aaacccttgc ggacggaagc ctgttccgcc tctttcgtca tgttgtgcga ccagtccgca
     7321 ccgccgtaaa gcagccagtc tttcggcgcg gaatacatca gcgtcgcgcc cgccagcggc
     7381 atatggctgt cgtatcgggc ggcggtgcgg tcttcctgat aacgcttcca catattgccc
     7441 acgttcagcg tcagccgcca gcgttcgctc aagcgtcggg agaaatcggc attgaagccg
     7501 ccgacgaaat tgtatcggct gccgcctaag aggttttgcc cgataaacgg cacgatgccg
     7561 aacgagcgcg ttaccgaacg atttttatag ccgagcgaca ggcgcaggca ctgttcgctg
     7621 aaatctttgt tatcccaata atgcacgccg ccgccgctga tgccgccgta gaggaaatga
     7681 tgcccgcccg cattgatttc gcgcgacacg cctaagccct agcgcaaacc gtgtgccttt
     7741 tgcggcaggc tgtcggcggt tttcgtccag cttctgcccg caaattcaat cgtttttttg
     7801 gacgaagcgt tgtttatag
//
LOCUS       DAWHSK010000074         7798 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.074, whole genome shotgun sequence.
ACCESSION   DAWHSK010000074 DAWHSK010000000
VERSION     DAWHSK010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7798
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.074"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(32..451)
                     /locus_tag="WIC03_001005"
                     /pseudo
     CDS             complement(32..451)
                     /locus_tag="WIC03_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690617.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(509..874)
                     /locus_tag="WIC03_001006"
     CDS             complement(509..874)
                     /locus_tag="WIC03_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883522.1"
                     /translation="MEKPLLTPPFFLFEDVSLDIFQGLSELEKKIEPQDLMDDVYRAF
                     DSVGNILNFRIVEKEQKGFWVSTKIKTVVFDSADMSSDDLFLKCLQSSYKAYFETEPA
                     GLDKRQLMKTLIQKCGFSC"
     gene            complement(856..1401)
                     /locus_tag="WIC03_001007"
     CDS             complement(856..1401)
                     /locus_tag="WIC03_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883523.1"
                     /translation="MKKQITAAVMMLSMIAPTMANGLDNQAFEDQVFHTRADAPMQLA
                     ELSQKEMKETEGAFLPLAILGGAAIGMWTQHGFSYATTGRPASVRDVAIAGGLGAIPG
                     GVGAAGKVVSFAKYGREIKIGNNMRIAPFGNRTGHPIGKFPHYHRRVTDNTGKTLPGQ
                     GIGRHRPWESKSTDRSWKNRF"
     gene            complement(1489..1704)
                     /locus_tag="WIC03_001008"
     CDS             complement(1489..1704)
                     /locus_tag="WIC03_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883524.1"
                     /translation="MVNKYIKTAISITAISIFYLLTQESSGKTEEPSYFLMFMFLNSL
                     WFEENKTVMAAVTAMIAAHLIFVTLSD"
     gene            complement(1694..2089)
                     /locus_tag="WIC03_001009"
     CDS             complement(1694..2089)
                     /locus_tag="WIC03_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883525.1"
                     /translation="MKKQITAAVIMLSMIAPAMANGLDNQAFEDQVFHTRADAPMQLA
                     ELSQKEMKETEGAWGPVGAFIGGVGGGIGYMMNQQLSGSRFSPTAFTLAVGAGAFGGA
                     TAGPAGVVWGFNRSLATGMVTGVAQRYGK"
     gene            complement(2132..3001)
                     /locus_tag="WIC03_001010"
     CDS             complement(2132..3001)
                     /locus_tag="WIC03_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="meta-pathway of phenol degradation family
                     protein"
                     /protein_id="HEZ3883526.1"
                     /translation="MKRIFLSALPAILPLSAYADLPLTIEDIITDKGKWKLETSLTYL
                     NSENSRAALAAPVYIQTGATSFIPIPTEIQENGSNTDMLAGTLGLRYGLTGNTDIYGS
                     GSYLWHEERKLDGNGKTRNKRMSDVSLGISHTFLKDDKNPALISFLESTVYEKSRNKA
                     SSGKSWLIGATTYKAIDPIVLSLTAAYRINGSKTLSDGIRYKSGNYLLLNPNISFAAN
                     DRISLTGGIQWLGRQPDRTDGKRESSRNTSTYAHFGAGFGFTKTTALNASARFNVSGQ
                     SSSELKFGVQHTF"
     gene            3166..4089
                     /locus_tag="WIC03_001011"
     CDS             3166..4089
                     /locus_tag="WIC03_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEZ3883527.1"
                     /translation="MKKRNNPLPLLNGVKPSYLVLPHEKQFYGLPLLHFLCIRFPFVG
                     ADDWRRRLNSGFVVGSDGAALDEHTLFEPGKVMFYYRETSSEDEPRIPFEEKILHIDE
                     HLIVVDKPHFLPVIPSGRFLRETLLTRLRLRPELQHLNVEDITPLHRLDKDTAGVMLL
                     SHNPATRGAYQTMFQNKTVWKTYEALAPTRTDLPYPLDVVSRLVRGEKFFTTQEAEGE
                     PNAHTTVELIENRGEFSLYRLTPHTGKKHQLRVHMMGLGMPLLNDALYPVPSEAGSED
                     YRKPLKLLAKKIAFADPLSGRERVFCSGFCL"
     gene            complement(4167..4808)
                     /locus_tag="WIC03_001012"
     CDS             complement(4167..4808)
                     /locus_tag="WIC03_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEZ3883528.1"
                     /translation="MLKPETIHISGPAGILETIHIPSEQVLARGVAVINHPNPLQGGT
                     NTNKVIQTAAKALSKLGFHCYLPNLRGVGNSEGTHDYGRGETQDCLAVIDYARAQHPE
                     APEFALSGFSFGGYVATFAAQARIPDLLLLIGAAVCHYTGRPEPSAVPDVAKTLMIHG
                     AEDEVVEIGKALKWAEPQDLPVITIAGSTHFFHGKLIVLRDTILRFAPVCLNG"
     gene            complement(4863..5654)
                     /locus_tag="WIC03_001013"
     CDS             complement(4863..5654)
                     /locus_tag="WIC03_001013"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEZ3883529.1"
                     /translation="MARHPYRRLRPAKSSFPEVGISEEGNIRSLHLGSDTIQSSMNLD
                     HPSELVLSYSRAMMGWLLFTDALPQHITQIGLGGGSFARWIDTYLPDTRQTAVDINPQ
                     VIAIARNLFELPFEGEKFEIIEADGAEYIKVFRHNTDVILVDGFDGEQIIDALVEEPF
                     FRDCRNALSSDGIFVTNWWSGDKRYQRFIERLLSVFEGRVLELPAESHGNVAVMAFQS
                     SPKEQNIDKLKKRADKLSNAYGLDFHRMLADLKASNPNNGKHFYL"
     gene            5820..5895
                     /locus_tag="WIC03_001014"
     tRNA            5820..5895
                     /locus_tag="WIC03_001014"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5853..5855,aa:Thr,seq:tgt)
     gene            5930..6005
                     /locus_tag="WIC03_001015"
     tRNA            5930..6005
                     /locus_tag="WIC03_001015"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5963..5965,aa:Thr,seq:tgt)
     gene            6147..6938
                     /gene="panB"
                     /locus_tag="WIC03_001016"
     CDS             6147..6938
                     /gene="panB"
                     /locus_tag="WIC03_001016"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEZ3883530.1"
                     /translation="MITVNTLQKMKAVGEKIAMLTAYESSFAALMDDAGVEMLLVGDS
                     LGMAVQGRKSTLPVSLRDMCYHTECVARGAKNAMIVSDLPFGAYQQSKEQAFAAAAEL
                     MAAGAHMVKLEGGVWMAETTEFLQMRGIPVCAHIGLTPQSVFAFGGYKVQGRGGKAQA
                     LLNDAKAHDDAGAAVVLMECVPAELAKKVTETVSCPTIGIGAGVDCDGQVLVMHDMLG
                     IFPGKTAKFVKNFMQGHNSVQAAVRAYVAEVKAKTFPAAEHIFAD"
     gene            7061..>7798
                     /locus_tag="WIC03_001017"
     CDS             7061..>7798
                     /locus_tag="WIC03_001017"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEZ3883531.1"
                     /translation="MQIIHTIRELRAWRKNAGKVAFVPTMGNLHEGHLALVREAKKRA
                     DSVVVSIFVNRLQFGQGEDFDKYPRTLQQDADKLEAEGVAVVFAPDEKELYPNVEQRY
                     NVEPPNLQNELCGKFRPGHFRGVATVVSKLFNIVAPDVACFGKKDYQQLAVIKGLTED
                     LNFDVEIVPVDTGRAEDGLALSSRNQYLSAAERDEAPRLYRELKAVAESLAQGSLDYA
                     GLERQAADHLTAAGWVVDYVEIRRADTL"
BASE COUNT         1830 a         1767 c         2102 g         2099 t
ORIGIN      
        1 ttcagcacct tagagaatcg ttctctttga gctaaggcga ggcaacgccg tactggttta
       61 aatttaatcc gctatacatc tcaatacgat aaatatttct taagtcaaaa tgcaagcctg
      121 accgtacctt aactgtcaaa attttattat tttttattta ttttaaaaca atttctgcaa
      181 aattctcttc gctttctctc ttttttagaa gcacataaga aaaaataaaa cttccccgat
      241 taaattcata aatatgtttc aaccatttgc ctcctctttc tgtaagacaa gattcagttt
      301 cattcttcct tattgtataa atatttcctt cacaaaatct gaaataaatc cataaatcca
      361 tcttatccat aattaaagaa aaagtttcac ctcgagattt tgtcaacaat tcgcaaggtt
      421 gcgatgttgc aatcaaatag ccgaaagaca ttttttacct catacatggt cgaaatcagt
      481 ttctgttagt tcagaatcca ttttttcgtc aacaactgaa tccgcatttt tgaattaacg
      541 ttttcatcag ctgccgttta tctaaaccgg caggttcagt ttcaaaataa gccttatatg
      601 aagactgtaa gcatttcaga aaaagatcat cagaagacat atctgccgaa tcaaatacaa
      661 ctgttttgat tttggtactt acccaaaacc ctttttgctc tttttctact atacggaaat
      721 tcagaatatt tccaaccgaa tcaaaagcac ggtaaacatc atccatcaaa tcctgcggct
      781 ctattttctt ttccaattcc gacaatcctt gaaatatatc caaagacaca tcttcaaata
      841 gaaaaaaagg aggagttaga agcggttttt ccatgatctg tccgtagatt ttgattccca
      901 agggcgatga cgaccaattc cctgtccagg caaagtcttg cccgtattat ccgtaactcg
      961 acgatgataa tggggaaatt ttccaatagg atgacctgtt ctattaccga aaggggctat
     1021 ccgcatatta ttgccgattt taatctcacg tccatattta gcaaaggaaa caacctttcc
     1081 tgcggcacct acaccaccag gaattgcgcc taatccgcca gcaatagcaa catctctaac
     1141 agaagctggt ctgcctgtcg ttgcataact aaaaccatgc tgtgtccaca taccaatggc
     1201 agcaccaccc aagatagcca atggaagaaa cgccccctca gtctccttca tctccttttg
     1261 agaaagctcc gccaactgca tcggtgcatc tgcccgcgtg tggaacactt ggtcttcaaa
     1321 tgcctgattg tccaatccgt ttgccattgt gggggcaatc atagacagca tcatcacggc
     1381 tgcggtgatt tgttttttca taacaaattc ttagggagga gataatgttt catcaaagtc
     1441 ctcctgaaag gctgcctgaa atttccaagc agcctttttg tttgcttatt aatcggatag
     1501 tgtgacaaat ataaggtggg cggcaatcat tgccgtaacg gcagccataa ctgttttatt
     1561 ttcttcaaac caaagcgagt tgagaaacat gaacatcaag aaatatgatg gttcttcagt
     1621 tttgccgctg ctttcttgag ttagaagata aaatatagat atggcggtta tgctgattgc
     1681 tgtttttata tatttattta ccatatcgtt gggcaacacc cgtaaccata cctgttgcca
     1741 aagatcggtt aaatccccat acaacccctg ccggacctgc tgtcgcccct ccaaaagctc
     1801 ccgcccctac ggcaagagtg aatgctgtgg gagaaaatct gcttccgcta agttgctggt
     1861 tcatcatata tccgattccg cctccgactc ctccgataaa tgcacctacc ggtccccaag
     1921 ccccctcagt ctccttcatc tccttttgag aaagttccgc caactgcatc ggcgcatctg
     1981 cccgcgtgtg gaacacttgg tcttcaaatg cctgattgtc caagccgttt gccattgcgg
     2041 gggcaatcat agacagcatt atcacggctg cggtgatttg ttttttcata ataactcctt
     2101 tggattacaa ggttggaaaa tcaaaacctg cttaaaatgt atgctgtacg ccaaatttca
     2161 gttcggaact gctttgccct gaaacgttga aacgtgcgga tgcgtttaaa gccgtggttt
     2221 tggtgaaacc gaaacctgcg ccgaaatggg cataggtgga tgtgtttctg gaggattccc
     2281 gtttgccgtc cgtccggtcg ggctgcctgc ccaaccattg gatgcctccg gtcaggctga
     2341 ttctgtcgtt ggcggcaaat gagatgttgg ggttgagcag caggtagttg cccgatttgt
     2401 agcggatgcc gtctgaaagg gttttgctgc cgttgatgcg gtaggcggcg gtgagggaaa
     2461 ggacaatcgg atctatggct ttgtaggtgg tggcgccgat gagccacgat tttcccgacg
     2521 aggctttgtt gcgcgatttt tcgtaaaccg tgctttcaag aaagctgatt agggcagggt
     2581 ttttgtcgtc tttaaggaag gtgtggctga tgccgaggga tacgtcggac atccgtttgt
     2641 tgcgggtttt gccgttgccg tcgagtttgc gttcttcgtg ccacagatag ctgccgctgc
     2701 cgtaaatgtc ggtattccca gtcagtccgt agcgcaaacc gagcgtgccg gcgagcatat
     2761 cggtattgct gccgttttct tggatttcgg tcggaatggg gataaacgag gttgcgccgg
     2821 tttgaatgta aaccggtgcg gcaagtgcgg cgcggctgtt ttcgctattc aggtaggtaa
     2881 gggaagtttc cagtttccat ttgcccttgt cggttattat gtcttcaatc gtcaagggca
     2941 ggtcggcata agcggataaa ggcaggatgg cgggcaaggc ggacaaaaag atacgtttca
     3001 tatttcctct tattgatatt aattcttatt taagaatagt aatgaatatc cgataattct
     3061 gtctaaataa atttttaaat caaaaaaaac gatatatttg tttttatcaa ttatgttttg
     3121 atatgccgtc tgaaaagttt gtgaaaaacg gttacaatcc gccgcatgaa aaaacgcaat
     3181 aatcctcttc cgttgttgaa cggtgtcaaa cccagttatt tggtgctgcc gcatgaaaag
     3241 cagttttacg ggctgccgct gctgcatttt ctgtgcatcc gctttccttt tgtgggcgcg
     3301 gacgattggc gcaggcggtt gaacagcggt tttgtggtcg gttcggatgg tgcggcgttg
     3361 gacgaacata ctttgttcga gccgggtaag gtgatgtttt attatcggga aaccagcagt
     3421 gaggatgagc cgcgtattcc gtttgaagaa aagattttgc atattgatga gcatttgatt
     3481 gtggtggaca agccgcattt tctgcccgtc atccccagcg ggcgtttttt aagggaaacg
     3541 ctgctcacac gtctgcgcct gcgtcccgaa ttgcagcatt tgaatgttga agacattacg
     3601 ccgctgcacc gcttggacaa ggatacggca ggcgtgatgt tgttgtcgca caatcctgcc
     3661 acgcgcgggg cttatcagac gatgtttcaa aacaaaacgg tatggaaaac gtatgaggcg
     3721 cttgcgccga caaggacgga tttgccgtat ccgctcgatg tggtttcgcg tttggtgagg
     3781 ggtgagaaat tttttacgac gcaagaggcg gaaggcgaac caaacgcaca tacgacggtc
     3841 gaactgattg aaaacagggg ggaattcagc ctttaccgcc ttacgccgca taccggcaag
     3901 aaacaccagt tgcgcgtgca tatgatgggt ttgggtatgc cgctgttgaa tgacgcgctc
     3961 tatcccgtgc cgtctgaagc gggcagcgag gattatcgga aacctttgaa gctattggca
     4021 aaaaagattg cgtttgcaga tcctttgtcg ggtcgggaaa gggtgttttg cagcggattt
     4081 tgcctataat gagagggaac gcaaacccgt atcggcggat gccgccgata cgggttttac
     4141 attatcgatg ccgtctgaaa ggggttttat ccgttcaggc atacgggcgc gaagcggagg
     4201 atggtgtcgc gcaggacgat gagtttgccg tgaaagaaat gcgtggaacc ggcgatggta
     4261 atgacgggca aatcttgcgg ttccgcccat ttcagtgctt tcccgatttc gacgacttcg
     4321 tcttccgcgc cgtgtatcat cagcgttttg gcaacatcgg gaacggcgga cggctcgggg
     4381 cggccggtat agtggcagac tgccgcgccg atgagcagca ataaatcggg tatgcgcgct
     4441 tgtgcggcaa atgtggcgac ataaccgccg aaggagaagc cggataaggc aaattcgggg
     4501 gcttcgggat gttgggcgcg ggcatagtcg atgacggcga ggcagtcttg cgtttcgccg
     4561 cgtccgtaat cgtgtgtgcc ttcgctgttg cctacgccgc gaaggttggg caggtagcag
     4621 tggaagccga gtttgcttaa ggctttggcg gcggtttgga tgactttgtt ggtgtttgtt
     4681 ccgccctgga gggggttggg atgattgatg acggcaacac cgcgtgccag tacttgttcg
     4741 gacgggatat ggatggtttc taaaatgccg gcaggaccgg atatgtgtat ggtttcgggt
     4801 ttcagcataa tgatttcctc ttgattttcc gcatgttccg atgcagccgt gaagatagtt
     4861 gcttaaaggt agaaatgctt gccgttgttg gggttggatg ctttcaggtc ggcgagcatg
     4921 cggtggaagt ccaatccgta tgcgttgctc agtttgtcgg cgcgtttctt gagtttgtct
     4981 atgttttgct ctttggggct gctttggaag gccattaccg cgacattgcc gtggctttcg
     5041 gcaggaagtt ccaagacgcg cccttcaaaa acgctcaaca accgttcgat gaagcgttgg
     5101 tagcgtttgt cgccgctcca ccagttggtt acgaatatgc cgtctgaaga gagtgcgttt
     5161 cggcagtctc ggaagaacgg ttcttcaacc agcgcatcga tgatttgttc gccgtcgaac
     5221 ccgtccacca aaatcacatc ggtgttgtgg cggaagactt tgatatattc tgcaccgtct
     5281 gcttcaataa tttcaaattt ctcgccctcg aaaggcaact caaacaggtt gcgggcaatg
     5341 gcgatgacct gcgggttgat gtccacggcg gtttggcgcg tgtcgggcag gtaggtgtct
     5401 atccagcgtg caaacgaacc gccgcccaag ccgatttggg tgatatgctg cggaagagcg
     5461 tcggtaaaca gcagccagcc catcatcgcg cggctgtaag agagcaccag ctcggacggg
     5521 tggtcgaggt tcatcgagct ttggatggtg tcgctgccca agtgaagcga acggatattg
     5581 ccttcttcgg aaatgccgac ttcgggaaag ctggattttg caggacgcag gcggcggtag
     5641 ggatgtcttg ccatcggtgt gaacggtcgg tttgaaaggc agatatttta tcagaatgcc
     5701 gatggttgtc ctgcttcaaa ttaatttctt ttcaaataaa ttacttattc ggatttgccg
     5761 gggctttcgg ataaattcct tgccaaggtg cggcattgcc tgcataattc gctttctttg
     5821 ccggtatagc tcagttggta gagcacctga cttgtaatca gggggtcccg agttcgactc
     5881 ttggtgccgg caccatcctt gcttatggtg cggtaataca atccaatatg ccggtatagc
     5941 tcagttggta gagcacctga cttgtaatca gggggtcccg agttcgactc ttggtgccgg
     6001 caccagattt gacagcccga ttgtgtaaaa cagtcgggct gttttgcatt tgcggcacgg
     6061 tcggtggcag ggctgcgggc atttcactat aatagcccgt ttcaaactga aaaggccgtc
     6121 tgaaaagggc ggggtaacaa tatctgatga ttactgtgaa cacactgcaa aaaatgaagg
     6181 cggtaggtga gaaaatcgcg atgctgaccg cttacgaatc cagttttgcc gcgctgatgg
     6241 acgatgccgg cgtggaaatg ctgctggtcg gcgactcttt gggtatggcg gttcaagggc
     6301 ggaaatcgac gctgcccgtc agcctgcgcg atatgtgcta tcacaccgaa tgtgtagcac
     6361 gcggtgcaaa aaatgcgatg attgtcagcg atttgccgtt tggtgcatat cagcagagta
     6421 aggagcaggc gtttgccgcc gccgccgaac tgatggctgc cggcgcgcat atggtcaaac
     6481 tcgaaggcgg tgtgtggatg gcggaaacga ctgaatttct gcaaatgcgc ggtattccgg
     6541 tttgcgcgca catcggtctg acaccgcaat ccgtgtttgc ttttggcggc tacaaagttc
     6601 aggggcgcgg cggcaaggcg caggctttgc ttaacgatgc caaggcgcac gatgatgcgg
     6661 gggcggcggt tgtattgatg gagtgtgttc ctgcggaact ggcaaaaaag gtaactgaaa
     6721 ctgtttcctg tccgactatc ggcatcggcg cgggggtcga ttgcgacggg caggttttgg
     6781 tgatgcacga tatgctcggt atttttccgg gcaagacggc gaaattcgtc aaaaacttca
     6841 tgcaggggca taatagcgtt caagcggcgg ttcgtgcgta tgttgccgaa gtcaaagcca
     6901 aaaccttccc tgctgcggaa catatttttg cagactgaac ggtttacatg cagcatgccg
     6961 tctgaaagcc gtttcagacg gcattttgtt ttgccgtgcg cggcgcgtat aatcggcgcg
     7021 ttttgtcggg caggaagccc gaaggataag gattaccgtt atgcaaatca tacataccat
     7081 tcgagaactg cgcgcgtggc gtaaaaatgc gggaaaggtg gcatttgtgc cgaccatggg
     7141 caatctgcat gaagggcatc ttgcgcttgt gcgtgaggcg aaaaaacgcg cggacagtgt
     7201 cgtggtcagc attttcgtca accgcctgca attcggtcag ggcgaggatt tcgacaaata
     7261 tccgcgtact ttgcaacagg atgcggacaa gctggaagcc gagggtgttg ccgttgtttt
     7321 cgcgcccgat gaaaaagaac tctatccgaa cgtggagcaa cggtataacg tcgaaccgcc
     7381 caacctgcaa aatgagttgt gcggcaagtt tcgtccggga catttccgcg gggtggcgac
     7441 ggtggtgtcc aagctgttca atattgtcgc gccggatgtt gcctgtttcg gcaaaaaaga
     7501 ctatcagcag cttgccgtga ttaaaggttt gaccgaggat ttgaattttg atgttgaaat
     7561 agtgcctgtc gatacggggc gcgcggaaga cggtttggca ctctccagcc gcaaccagta
     7621 tttgagtgcg gcggaacgcg acgaagcacc gcgcctgtac cgcgaattaa aggctgttgc
     7681 cgaatccttg gcgcagggca gtttggatta tgcaggtttg gagcggcagg ctgccgacca
     7741 tttgaccgct gcgggctggg tggtcgatta tgtcgaaatc cgccgcgccg atacgctc
//
LOCUS       DAWHSK010000075         7748 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.075, whole genome shotgun sequence.
ACCESSION   DAWHSK010000075 DAWHSK010000000
VERSION     DAWHSK010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7748
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.075"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..3686)
                     /gene="hrpA"
                     /locus_tag="WIC03_001018"
     CDS             complement(<1..3686)
                     /gene="hrpA"
                     /locus_tag="WIC03_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-partner secretion exoprotein HrpA"
                     /protein_id="HEZ3883532.1"
                     /translation="MNKGLHRIIFSKKHSTMVAVAETANSQGKGKQAGSSVSVSLKTS
                     GDLCGKLKTTLKTLVCSLVSLSMVLPAHAQITTDKSAPKNQQVVILKTNTGAPLVNIQ
                     TPNGRGLSHNRYTQFDVDNKGAVLNNDRNNNPFVVKGSAQLILNEVRGTASKLNGIVT
                     VGGQKADVIIANPNGITVNGGGFKNVGRGILTTGAPQIGKDGALTGFDVRQGTLTVGA
                     AGWNDKGEADYTGVLARAVALQGKLQGKNLAVSTGPQKVDYASGEISAGTAAGTKPTI
                     ALDTAALGGMYADSITLIANEKGVGVKNAGTLEAAKQLIVTSSGRIENSGRIATTADG
                     TEASPTYLSIETTEKGAAGTFISNDGRIESKGLLVIDTGEDIGLRNGAVVQNNGNRPA
                     ATVLNAGRNLVIESKANVNNAKGSTNLSTGGRTVIKEASIQTGTTVYSSSKGNAELGN
                     NTRITGADVTVLSNGTISSSAVIDAKDTAHIEAGKPLSLEASTVTSDIRLNGGSIKGG
                     KQLALLADDNITAKTTNLNTPGNLYVHTGKDLNLNVDKDLSAASIHLKSDNAAHITGT
                     SKTLTASKDMGVEAGSLNVTNTNLRTNSGNLHIQAAKGNIQLRNTKLNAAKALETTAL
                     QGNIVSDGLHAVSADGHVSLLANGNADFTGHNTLTAKADVNAGSVGKGRLKADNTNIT
                     SSSGDITLVAGNGIQLGDGKQRNSINGKHISIKNNGGNADLKNLNVHAKSGALNIHSD
                     RALSIENTKLESTHNTHLNAQHERVTLNQVDAYAHRHLSITGSQIWQNDKLPSANKLV
                     ANGVLALNARYSQIADNTTLRAGAINLTAGTALVKRGNINWSTVSTKTLEDNAELKPL
                     AGRLNIEAGSGTLTIEPANRISAHTDLSIKTGGKLLLSVKGGNAGAPSAQVSSLEAKG
                     NIRLVTGETDLRGSKITAGKNLVVATTKGKLNIEAVNNSFSNYFPTQKAAELNQKSKE
                     LEQQIAQLKKSSPKSKLIPTLQEERDRLAFYIQAINKEVKGKKPKGKEYLQAKLSAQN
                     IDLISAQGIEISGSDITASKKLNLHAAGVLPKAADSEAAAILIDGITDQYEIGKPTYK
                     SHYDKAALNKPSRLTGRTGVSIHAAAALDDARIIIGASEIKAPSGSIDIKAHSDIVLE
                     AGQNDAYTFLKTKGKSGKIIRKTKFTSTRDHLIMPAPVELTANGITLQAGGNIEANTT
                     RFNAPAGKVTLVAGEELQLLAEEG"
     gene            complement(3795..5474)
                     /gene="hrpB"
                     /locus_tag="WIC03_001019"
     CDS             complement(3795..5474)
                     /gene="hrpB"
                     /locus_tag="WIC03_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-partner secretion system transporter HrpB"
                     /protein_id="HEZ3883533.1"
                     /translation="MEIINDAELIRSMQRQQHIDAELLTDANVRFEQPLEKNNYVLSE
                     DETPCTRVNYISLDDKTVRKFSFLPSVLMKETAFKTGMCLGSNNLSRLQKAAQQILIV
                     RGYLTSQAIIQPQNMDSGILKLRVSAGEIGDIRYEEKRDGKSAEGSISAFNNKFPLYR
                     NKILNLRDVEQGLENLRRLSSVKTDIQIIPSEEEGKSDLQIKWQQNKPIRFSIGIDDA
                     GGKTTGKYQGNVALSFDNPLGLSDLFYVSYGRGLAHKTDLTDATGTETASGSRSYSVH
                     YSVPVKKWLFSFNHNGHRYHEATEGYSVNYDYNGKQYQSSLAAERMLWRNRLHKTSVG
                     MKLWTRQTYKYIDDAEIEVQRRRSAGWEAELRHRAYLNRWQLDGKLSYKRGTGMRQSM
                     PAPEENGGGTIPGTSRMKIITAGLDAAAPFMLGKQQFFYATAIQAQWNKTPLVAQDKL
                     SIGSRYTVRGFDGEQSLFGERGFYWQNTLTWYFHPNHQFYLGADYGRVSGESAQYVSG
                     KQLMGAVVGFRGGHKVGGMFAYDLFAGKPLHKPKGFQTTNTVYGFNLNYSF"
     gene            5421..6404
                     /locus_tag="WIC03_001020"
     CDS             5421..6404
                     /locus_tag="WIC03_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HEZ3883534.1"
                     /translation="MLLTLHGTDKFCIVNNFHTNRNTAYKALKDACNNLFERQFSFIE
                     KTPKGEKVVRTRWVSQVAYIEQQATVELVFAPNVAPLITMLEKNFTSYELDQVSSLSS
                     KYAVRLYEIIISWRAAGKTPMFSTMELRERLGMMPDEYQKMELFKRKVLDFAVKQIND
                     KTDISITYEQHKEGRKIVGFTFSILHKIGSKDIPLENQSELFAGMTDLEAGTIRVRAE
                     AYIASLIAKGQNVTKAHRLNILKKAVEERWGFEDVAKDNGAKNPENKNAKVVLNEWEK
                     ISNGTRFKDKDGTIWVKDSGMLRTEGTNRWIADSQIAKLFPMLTVMVEEGI"
     gene            6501..6917
                     /locus_tag="WIC03_001021"
                     /pseudo
     CDS             6501..6917
                     /locus_tag="WIC03_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219448.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
     gene            complement(6923..7021)
                     /locus_tag="WIC03_001022"
                     /pseudo
     CDS             complement(6923..7021)
                     /locus_tag="WIC03_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611454.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            7114..>7748
                     /locus_tag="WIC03_001023"
     CDS             7114..>7748
                     /locus_tag="WIC03_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HEZ3883535.1"
                     /translation="MNRTLYKVVFNKHRNCMIVVAENAKREGKNTADTQAVGILPNDI
                     AGFAGFIHSISVISFSLSLLLGSALILTSSSANAQGIVADKSAPAQQQPTILQTGNGI
                     PQVNIQTPTSAGVSVNQYAQFDVGNRGAILNNSRSNTQTQLGGWIQGNPWLARGEARV
                     VVNQINSSHSSQMNGYIEVGGRRAEVVIANPAGIAVNGGGFINASRATLTT"
BASE COUNT         1882 a         1769 c         1891 g         2206 t
ORIGIN      
        1 atgccttctt ctgccagcag ttgcagctct tcacccgcaa ccagggtaac tttacctgca
       61 ggggcattga agcgggtggt attagcttcg atgttgccgc ctgcctgaag cgtgataccg
      121 ttggcggtca gctcgacggg ggctggcata atcaggtggt cgcgggtgct ggtaaacttg
      181 gtttttctga tgattttgcc gcttttacct ttggttttta agaaggtata ggcatcgttt
      241 tgtccagcct ccagtacaat atcactatgg gctttgatgt ctatgctgcc tgagggagct
      301 ttgatttcgg atgcaccgat aataatacgt gcatcatcga gtgccgcagc tgcatgaata
      361 cttacccccg tacgtccggt caaacgtgaa ggcttgttca gagcagcttt gtcgtagtga
      421 ctcttgtagg tgggcttgcc aatttcatat tggtcggtta tgccgtcaat cagaatagca
      481 gccgcctctg aatctgctgc ctttggcaat acgcctgcgg cgtgaaggtt cagttttttg
      541 gaagcggtaa tatcggaacc gctgatttcg atgccttgtg cggaaatcaa gtcaatattt
      601 tgtgcagaaa gcttggcttg caggtattct ttgcctttgg gttttttacc tttaacttcc
      661 ttgttgatgg cttgaatata gaaagcgaga cggtcgcgtt cttcttgcag ggttggaatc
      721 agcttgcttt taggcgagct ttttttcaac tgcgcaatct gctgttccaa ttctttggat
      781 ttttggttga gttcagccgc tttttgtgta ggaaaataat tgctgaatga gttgtttacg
      841 gcttcgatat tcaacttgcc tttggtggtg gcgacaacca agtttttacc ggctgtaatt
      901 ttagaacctc ttaaatctgt ttctcctgta accagacgga tattgccttt tgcttccaat
      961 gaggaaactt gagcactagg cgcacctgca tttcctcctt ttacagacaa cagcaatttt
     1021 ccgcctgttt tgatgctcag gtcggtatgc gcactgatgc ggttggcagg ttcgatggtt
     1081 aatgtgccgc tacctgcttc aatattcagc cgtccggcca atggttttaa ttcggcatta
     1141 tcttccaaag ttttggtcga aacggtactc caattgatgt tgccgcgctt gactagggcg
     1201 gtaccggcag taaggttgat tgcacccgct ctcagcgtgg tgttgtcggc aatttgggaa
     1261 tagcgcgcat tgagtgccaa tacaccgtta gccaccagct tgttggcaga aggcagtttg
     1321 tcgttttgcc aaatctggct gccggtaatg cttagatgac ggtgtgcgta ggcatctact
     1381 tggttgagcg ttacccgctc gtgttgtgca ttaagatgcg tattatgggt agactccagc
     1441 ttggtatttt ctatgctcaa tgcccggtcg gaatgaatgt tcaatgcccc gcttttggca
     1501 tggacgttaa ggttttttaa gtcggcatta ccaccgttgt ttttgatgct gatgtgtttt
     1561 ccgttgattg aattgcgttg ttttccgtca ccaagctgaa taccgttgcc ggcaaccaac
     1621 gtaatatctc ctgaagatga agtgatattg gtattgtctg ctttcagacg gcctttacca
     1681 accgatcctg cattgacatc ggccttggct gtcagggtat tgtgaccggt aaagtcggca
     1741 ttaccgttgg ccaataagga tacatgaccg tctgcagaaa cagcatgaag gccgtctgaa
     1801 acgatattgc cctgcaatgc ggtggtttcg agagccttgg ctgcgttcag cttggtattg
     1861 cgaagctgaa tattgccttt ggctgcctga atgtgcagat tacccgagtt ggtacgcaga
     1921 ttggtattgg taacattcag cgagcctgcc tccacaccca tgtcttttga ggcagtgagg
     1981 gttttactgg tgccggtaat atgggcagcg ttatccgatt tcaaatggat gctggcggca
     2041 gacaaatctt tatcaacatt caaattcaga tctttacctg tatgaacata cagattgccg
     2101 ggagtattca gattggtagt tttggcagta atgttatcgt ctgccagtaa agcaagctgc
     2161 ttgccgccct tgatactgcc tccgtttaag cggatatcgg aggtaactgt tgaagcttcc
     2221 aaagaaagcg gtttgcctgc ttcgatgtgt gcggtgtctt tggcatctat tacggcggaa
     2281 ctgctgatgg tgccgttgga taatacggta acatctgccc cggtaatgcg tgtgttattg
     2341 cctaattcgg cgttgccttt gctggaactg tatacggtag tgccagtctg aatactggcc
     2401 tccttgatga cggtacggcc gccggtcgac agattggtcg aacctttagc attgttcaca
     2461 tttgctttgc tttcgataac caaattacgg ccggcattta ataccgtagc agcagggcgg
     2521 ttgccgttgt tttgaacgac cgctccgtta cgcaaaccga tgtcctcccc ggtatcaata
     2581 accaataagc ctttgctctc gatccgacca tcattggaga taaatgtgcc tgccgctcct
     2641 ttttcggtgg tttcgatgga gagataagtc ggtgaagctt cggtgccgtc ggcagtggtg
     2701 gcgatgcggc cgctgttttc aatgcggcct gacgaagtca caatcaattg cttggccgct
     2761 tcgagtgtgc cggcattttt gacgcctacg cctttttcat tggcaatcag tgtgatgctg
     2821 tcggcgtaca taccgcccag tgcggcagta tcaagggcaa tagtcggttt cgtacccgct
     2881 gccgtacctg cactgatttc gccgctggcg taatctactt tctgaggacc ggtagaaacc
     2941 gccaggtttt taccctgtaa tttcccctgc aaagcaactg cacgagcaag taccccggtg
     3001 tagtcggctt cgcctttatc attccaacct gctgctccta cggtcaatgt gccttgacgc
     3061 acatcaaatc ctgtcagtgc accgtctttg ccgatttggg gcgcaccggt agttaagatg
     3121 ccccgaccga catttttaaa gccgccgcca ttaacggtaa tgccgttggg gttggcaata
     3181 atcacgtcgg ccttttgacc gcctacggta acgatgccgt tgagtttgct agccgtaccg
     3241 cgtacctcgt tcaaaatcaa ttgcgcactg cctttgacca caaacggatt attgttacgg
     3301 tcgttgttta acactgcccc tttgttgtca acatcaaact gcgtatagcg gttgtggctc
     3361 aatccgcgtc cattcggagt ttggatattc accaaggggg caccagtgtt ggttttaagg
     3421 ataacgacct gctggttttt aggtgctgat ttgtcggtgg taatttgggc atgggcaggc
     3481 aataccatac tcagggaaac caaagagcag accaaagttt taagggtggt tttgagtttg
     3541 ccgcaaaggt cgcctgaagt tttcagtgaa acagaaaccg aactgcctgc ctgtttacct
     3601 ttgccctggc tgttggcagt ttcggctact gcaaccatgg tgctgtgctt tttactaaag
     3661 ataatgcgat gtaaaccttt attcatgtct attccatttt gaagatgaac gtactgcgcg
     3721 ccaagtacgt aggtaaagtt tgacggtctg aggataagga aagaccgtct aaatatcagt
     3781 aaaaaattca gaggttagaa actgtaattc aagttgaagc cgtaaacggt gttggtcgtc
     3841 tgaaagcctt tgggtttatg aagcggcttg ccggcaaaca gatcataagc aaacataccg
     3901 cctactttat gccctcctct gaagccgacc actgcaccca tcagctgctt gcccgataca
     3961 tattgtgcac tttcgccaga tacgcggcca tagtccgcac cgagatagaa ctgatggttc
     4021 ggatgaaaat accaagttaa agtattctgc cagtagaaac ctcgctctcc gaaaagactc
     4081 tgctccccat caaatccgcg aacggtgtag cggctgccga ttgacaattt atcttgggca
     4141 accaacggcg ttttgttcca ttgagcttga atggcggttg cgtagaaaaa ctgctgtttg
     4201 cccaacataa acggggccgc tgcatccaat ccggcggtta tgattttcat acgggatgtg
     4261 cctggaatag taccgccgcc gttttcttca ggtgcgggca tactttggcg cataccggtc
     4321 ccgcgtttgt aagacaactt gccgtcaagc tgccaacggt tgaggtaagc acggtggcgc
     4381 aattcggctt cccagcctgc agagcggcgg cgttgcactt cgatttcggc atcgtcgatg
     4441 tatttatagg tttggcgtgt ccataatttc attccgactg aagttttatg aagtctgtta
     4501 cgccaaagca tgcgctcggc ggccaagctg ctctgatatt gtttgccgtt gtaatcgtaa
     4561 ttgacggaat agccttcggt tgcttcgtgg taacgatgtc cattgtgatt aaaagaaaac
     4621 agccattttt ttacgggcac cgaataatgc acgctgtaac ttctggatcc gcttgcagtt
     4681 tccgtaccgg tggcatcagt caagtccgtt ttgtgcgcca aaccgcgtcc atatgaaaca
     4741 taaaacaaat cgcttaagcc caaagggtta tcgaacgata aagcgacatt tccttgatat
     4801 ttgccggtcg ttttgccgcc cgcatcatct ataccgatac tgaaccgtat gggtttattc
     4861 tgctgccatt tgatctgtaa atcgcttttg ccttcttctt cggacggtat aatctgaata
     4921 tctgttttaa cactcgacaa acgacgcagg ttttccaagc cctgctctac atcgcgaaga
     4981 ttgagaattt tgttcctata taagggaaat ttgttattga atgcactaat actgccttcg
     5041 gcagacttcc catcccgttt ttcttcatag cggatatccc ctatttcgcc tgctgatacc
     5101 cgtaatttca gaattcccga atccatattc tgtggttgga taatagcttg ggaagtgagg
     5161 tagccacgca caatcagtat ctgttgcgcg gctttttgta gcctgctcaa attattggaa
     5221 cctaaacaca tcccagtttt aaaagctgtt tctttcatga gcacagaagg aagaaaagaa
     5281 aatttgcgca ccgtcttatc atctaaacta atgtaattta cccgagtaca cggtgtttca
     5341 tcttcactca ggacataatt gttcttctcc aatggttgct cgaaacggac atttgcatca
     5401 gttaacaatt cagcatctat gtgctgctga cgctgcatgg aacggataag ttctgcatcg
     5461 ttaataattt ccataccaac cgtaatacgg cgtataaggc tttgaaagat gcctgtaaca
     5521 acctttttga acgccaattc agttttatcg aaaaaacacc aaaaggggaa aaggtagtac
     5581 ggacaaggtg ggtatctcaa gtcgcctata ttgaacaaca ggcaacggta gagttagttt
     5641 ttgcaccaaa tgttgcccct ttgattacga tgctagaaaa aaacttcaca agctacgagc
     5701 ttgatcaggt ctcatcgttg agcagtaaat acgcggtgcg gctctacgaa attattattt
     5761 catggcgtgc agccggtaag acaccgatgt tcagtacaat ggagttgcgc gaacgtttgg
     5821 gtatgatgcc tgacgagtat caaaaaatgg agctattcaa acgtaaggtt ttggatttcg
     5881 ccgtcaagca gatcaatgat aaaacggata tttccattac ctacgagcag cataaagaag
     5941 gacgaaaaat tgtaggtttt acattctcaa tcctacataa aatagggtcg aaagacatcc
     6001 ctcttgaaaa tcaatcggaa ctttttgccg gaatgactga tttggaagcc ggaacgatac
     6061 gggtcagggc ggaagcatat atcgcttcgc tcattgcaaa aggtcagaac gtgactaaag
     6121 cccataggct gaatattctg aaaaaagccg tagaggaacg ttggggattt gaagatgtgg
     6181 ctaaagataa tggggccaaa aatcctgaaa acaaaaatgc aaaagtcgtt ttaaacgaat
     6241 gggaaaagat ttctaatggc acacgtttta aggacaagga tggaacaatt tgggttaaag
     6301 attcaggtat gcttaggact gaagggacaa acaggtggat agccgattct cagattgcca
     6361 aattatttcc tatgttaact gtgatggttg aggaaggtat ttaacccggc aactcgcgta
     6421 ttctacctgt tttgcggtac ggaaaccaat tgaacctgct ttacgctaca atagaagatt
     6481 gcaattttgt cggatgtacc atgaacgatt acacagccat gccgtctgaa gacggaggaa
     6541 tcggctcatt atcgcttccg ccgcactcaa tggaggcgga acaatccgtt ttgggcgggt
     6601 tgatgctgga aaatccggct tgggacagga ttgccgatgt ggtttcggga gaggatttct
     6661 accgccacga acaccgcctg attttccgat ccattgccaa actgatcaat gagagccgtc
     6721 ctgccgatgt catcacggtt caggaagatt tgcagcggaa cgaagagctg gaagcggcgg
     6781 ggggattcga atatctgatt acgctggcgc aaaatactcc gtctgccgcc aacatccggc
     6841 gccatgccga aatcgtgcgc gagcgttcca ttatgcgcca actcgccgaa gtgggaacgg
     6901 aaattgcccg cagcgccata cccaaaatat cggcagcgga acgggcggta gcttggagta
     6961 cgaaaaattc aagcagtttc ttttgaactc tattgtttaa tttacaattg gttatcttta
     7021 ttttcgtagc ctggcataac tgttaatcta cgtcagcccc taaaaattaa tctaatttac
     7081 gattataaaa atattattaa gaaagccctt actatgaacc gcaccctgta caaagttgta
     7141 tttaacaaac atcgaaactg catgatagtc gttgctgaaa atgccaaacg cgagggcaaa
     7201 aacacagccg acacccaagc tgtaggtatt ttgccaaatg atattgcggg ctttgcgggt
     7261 tttatccatt ctatctctgt tatctcattc tccctttcat tactgctcgg ttctgccctt
     7321 atcctgactt cttcttctgc taatgcccaa ggtatcgttg ccgacaaatc cgcacctgca
     7381 cagcaacagc ctaccatcct gcaaacaggt aacggcatac cgcaagtcaa tattcaaacc
     7441 cctacttcgg caggggtttc tgttaatcaa tacgcccagt ttgatgtggg taatcgcggg
     7501 gcgattttaa acaacagccg cagcaacacc caaacacagc taggcggttg gattcaaggt
     7561 aatccttggt tggcaagggg cgaagcacgt gtggttgtaa accaaatcaa cagcagccat
     7621 tcttcacaaa tgaatggcta tattgaagtg ggcggacgac gtgcagaagt cgttattgcc
     7681 aatccggcag ggattgcagt caatggtggt ggttttatca atgcttcccg tgctactttg
     7741 acgacaag
//
LOCUS       DAWHSK010000076         7734 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.076, whole genome shotgun sequence.
ACCESSION   DAWHSK010000076 DAWHSK010000000
VERSION     DAWHSK010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7734
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.076"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..325
                     /locus_tag="WIC03_001024"
     CDS             <1..325
                     /locus_tag="WIC03_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883536.1"
                     /translation="AEITLETPDSNTSEADALPDFLKDGEEETVDWSIYLSEENIPNN
                     ADTSFPSESVGSDAPSEAKYDLAEMYLEIGDRDAAAETVQKLLEEAEGDVLKRAQALA
                     QELGI"
     gene            complement(498..914)
                     /gene="gloA"
                     /locus_tag="WIC03_001025"
     CDS             complement(498..914)
                     /gene="gloA"
                     /locus_tag="WIC03_001025"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEZ3883537.1"
                     /translation="MRLLHTMLRVGNLEKSLDFYQNVLGMKLLRRKDYPEGRFTLAFV
                     GYGDETDSTVLELTHNWDTERYDLGNAYGHIAVEVDDAYEACERVKRQGGNVVREAGP
                     MKHGTTVIAFVEDPDGYKIEFIQKKSGDDSVAYQTA"
     gene            complement(939..2108)
                     /gene="lapB"
                     /locus_tag="WIC03_001026"
     CDS             complement(939..2108)
                     /gene="lapB"
                     /locus_tag="WIC03_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HEZ3883538.1"
                     /translation="MDNELWIILLPIILLPVFFAMGWFAARVDMKTVLKQAKSIPSGF
                     YKSLDALVDRNSGRAARELAEVIDQQPQSYDLNLTLGKLYRQRGENDKAINIHRTMLD
                     SPDTVGEKRARVLFELAQNYQSAGLVDRAEQIFLGLQDGKMAREARQHLLNIYQQDRD
                     WEKAVETARLLSHDDQTYQFEIAQFYCELAQAALFKSNFDVARFNVGKALEANKKCTR
                     ANMILGDIEHRQGNFPAAVEAYAAIEQQNHAYLSMVGEKLYEAYAAQGKPEEGLNRLT
                     GYMQTFPELDLINVVYEKSLLLKCEKEAAQTAVELVRRKPDLNGVYRLLGLKLSDLDP
                     AWKADADMMRSVIGRQLQRSVMYRCRNCHFKSQVFFWHCPACNKWQTFTPNKIEV"
     gene            complement(2119..2433)
                     /locus_tag="WIC03_001027"
     CDS             complement(2119..2433)
                     /locus_tag="WIC03_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HEZ3883539.1"
                     /translation="MKLIYTVIKIIILLLFLLLAVINTDAVTFSYLPGQKVDLPLIVV
                     LFGAFVVGIVFGMFALFGRLLSLRGENGRLRAEVKKNARLTGKELTAPPAQNAPESAK
                     QP"
     gene            2743..3741
                     /gene="ilvE"
                     /locus_tag="WIC03_001028"
     CDS             2743..3741
                     /gene="ilvE"
                     /locus_tag="WIC03_001028"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HEZ3883540.1"
                     /translation="MSRPVPAVFGSVFHSQMPVLAYREGKWQPTEWQSSQDLSLAPGA
                     HALHYSSECFEGLKAFRQADGKIVLFRPTANIARMRQSADILHLPRPETQAYLDALIE
                     LVKRAADEIPDAPAALYLRPTLIGTDPVIGKAGSPSETALLYILASPVGDYFKVGSPV
                     KILVETEHIRCAPHMGRVKCGGNYASAMHWVLKAKAEYGANQVLFCPNGDVQETGASN
                     FILINGDEIITKPLTDEFLHGVTRDSVLTVAKDLGYTVSERNFTVDELKAAVENGAEA
                     ILTGTAAVISPVTSFVIGGKEIEVKSQERGYAIRKAITDIQYGLAEDKYGWLVEVC"
     gene            complement(3985..4770)
                     /gene="fabI"
                     /locus_tag="WIC03_001029"
     CDS             complement(3985..4770)
                     /gene="fabI"
                     /locus_tag="WIC03_001029"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEZ3883541.1"
                     /translation="MGFLQGKKILITGMISERSIAYGIAKACREQGAELAFTYVVDKL
                     EERVRKMAAELGSELVFRCDVASDDEINQVFADLGKHWDGLDGLVHSIGFAPKEALSG
                     DFLDSISREAFNTAHEISAYSLPALAKAARPMMRGRNSAIVALSYLGAVRAIPNYNVM
                     GMAKASLEAGIRFTAACLGKEGIRCNGISAGPIKTLAASGIADFGKLLGHVAAHNPLR
                     RNVTIEEVGNTAAFLLSDLSSGITGEITYVDGGYSINALSTEG"
     gene            complement(4925..5746)
                     /gene="dapD"
                     /locus_tag="WIC03_001030"
     CDS             complement(4925..5746)
                     /gene="dapD"
                     /locus_tag="WIC03_001030"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEZ3883542.1"
                     /translation="MSLQNIIETAFENRADINPTTVTPEVKEAVLETIRQLDSGKLRV
                     AERLGVGEWKVNEWAKKAVLLSFRIQDNEILNDGVNKYFDKVPTKFADWSEDEFKNAG
                     FRAVPGAVARRGSFVAKNVVLMPSYVNIGAYVDEGAMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQASPTIIEDNCFIGARSEIVEGVIVEEGSVISMGVFIGQSTKIFDRT
                     TGEIYQGRVPAGSVVVSGSMPSKDGSHSLYCAVIVKRVDAQTRAKTSVNELLRGI"
     gene            complement(5756..5947)
                     /locus_tag="WIC03_001031"
     CDS             complement(5756..5947)
                     /locus_tag="WIC03_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883543.1"
                     /translation="MPSERRRRGIKAIPAACPIPKRQCSATARVLLTNMRLRGTIAHS
                     FQMNILPENTGKRPTKHLT"
     gene            complement(5917..7560)
                     /gene="pgi"
                     /locus_tag="WIC03_001032"
     CDS             complement(5917..7560)
                     /gene="pgi"
                     /locus_tag="WIC03_001032"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEZ3883544.1"
                     /translation="MNAFTRAWYALERHNQDTRHVLLRDRFACEPDRFERMHERLDGM
                     LFDYSKNRLGEDTLQLLCRLAETADLEGKMRALRTGAKVNGSEGRAALHTALRLPDGA
                     DAVYADGRDVLPEIRRELNRALKFAHSLDDGSYQGTTGKRITDFVHIGIGGSDLGPAM
                     CVQALEPFRRHIAVHFAANADPACLDAVLCRLNPETTVFCVASKSFKTPETLLNAQAV
                     KAWYRGAGFSESETACHFCAVSADTAAAAAFGIAAERVFAMYDWVGGRYSVWSPVGLP
                     VMVAVGGARFRELLAGAHAMDRHFFSTPTRHNIPVLMALIAVWYNNFQQADGQTAVPY
                     SHNLRLLPAWLNQLDMESLGKSRASDGSPAACKTGGIVFGGEGVNCQHAYFQLLHQGT
                     RLIPCDFIVPMTAQGVEDGRSRFTVANAFAQAEALMKGKTLDEARAELADLPEAERER
                     LAPHKEFPGNRPSNSILLDRLTPYNLGMLMAAYEHKTFVQGVIWDINPFDQWGVEYGK
                     QLAKTIIGELEGGTSVHDASTEGLMAFYRECRLKGGGAA"
BASE COUNT         1727 a         2153 c         2084 g         1770 t
ORIGIN      
        1 agcggaaatc actttggaaa cgcctgattc caacacctct gaggcagacg ctttgcccga
       61 cttcctgaaa gacggcgagg aggaaacggt agattggagc atctacctct cggaagaaaa
      121 tatcccaaat aatgcagata ccagtttccc ttcggaatct gtaggttctg acgcgccttc
      181 cgaagcgaaa tacgaccttg ccgaaatgta tctcgaaatc ggcgaccgcg atgccgctgc
      241 cgagacagtg cagaaattgc tggaagaagc ggaaggcgac gtactcaaac gtgcccaagc
      301 attggcgcag gaattgggta tttgattccc aactgccctt tcgcagatca aggatgccgt
      361 ttcagacggc atcttttttt gccttatcgg tgtaacggat aaagtttgaa ccggcacagg
      421 ctcaaacagc aggtcgacgg caacaaaatg ccgtctgaac cccctaaagg cttcagacgg
      481 cattggcggc ggcggtatca ggcagtttga taggcaaccg aatcgtcgcc gcttttcttt
      541 tgaatgaact cgattttgta tccgtcgggg tcttcgacga aggctatcac ggttgtgccg
      601 tgtttcatcg ggccggcttc gcggacgacg tttccgccct gccgcttcac acgttcgcag
      661 gcttcgtagg catcgtccac ttcaaccgcg atgtgtccgt aggcgttgcc caagtcgtat
      721 cgttccgtat cccagttgtg cgtcagttcc aaaaccgtgc tgtcggtttc atcgccgtaa
      781 ccgacgaagg caagggtaaa tctgccttcg ggataatctt ttcggcggag cagtttcata
      841 cccaaaacgt tttggtagaa atcgagggat ttttcgagat tgcccacgcg gagcatagta
      901 tggagtaagc gcattttttg tgttcctttc ggtggtggtt aaacttcgat tttattcggg
      961 gtaaacgtct gccatttgtt gcaggcgggg cagtgccaga aaaagacttg tgatttgaag
     1021 tggcagtttc ggcaccggta catcacgctg cgctgtagct gccgtccgat aaccgaacgc
     1081 atcatatcgg catcggcttt ccaagccgga tccaaatcgc tgagtttcaa accaagcagg
     1141 cggtacacgc cgttgaggtc gggcttgcgg cggacaagct cgacggcggt ttgcgcggct
     1201 tctttctcgc acttaagcag cagggatttc tcgtacacga cattgatcag gtcaagttcg
     1261 ggaaacgtct gcatatatcc tgtcagacgg ttcaagcctt cttcaggttt tccctgcgcg
     1321 gcataggctt cgtaaagctt ctcgccgacc atgctcaagt atgcatggtt ttgctgctcg
     1381 atggcggcat aggcttcgac ggcggcaggg aaattgcctt gtcggtgttc gatgtcgccc
     1441 aaaatcatgt tggcacgggt gcattttttg ttggcttcga gtgccttgcc gacattgaaa
     1501 cgcgcgacat cgaaattgga cttgaacagc gcggcttggg caagttcgca ataaaactgg
     1561 gcgatttcaa actgataggt ctgatcgtca tggctgagca gccgggcggt ttcaaccgct
     1621 ttttcccaat ccctgtcctg ttggtagata ttgagcaggt gctgtctggc ttcacgcgcc
     1681 attttaccgt cttgcagccc caaaaaaatc tgttcggcac gatcgaccaa ccccgcactt
     1741 tggtagtttt gcgccaattc aaacaggacg cgcgcgcgct tttcgccgac cgtatcggga
     1801 gaatcgagca ttgtccggtg tatgttgatg gctttgtcgt tttcgccacg ctgacggtaa
     1861 agcttgccta gggtgaggtt taggtcgtat gactgaggct gctgatcaat gacctctgcc
     1921 aattccctgg cggcacgtcc gctgttgcgg tcaaccaagg catccagact tttataaaat
     1981 cccgacggta tgctttttgc ctgctttaat acagtcttca tatccacacg ggcggcaaac
     2041 cagcccatcg cgaagaaaac gggcaaaagg ataatcggca gcaggataat ccacaattca
     2101 ttgtccatat cggctttctc aaggctgttt ggcagattcg ggcgcatttt gcgccggtgg
     2161 tgcggtcagc tctttccccg tcaaacgcgc atttttcttt acttcggcac gcaacctgcc
     2221 gttctcgcca cgtaacgaca acaaccgtcc gaacaaggca aacattccga acacgatgcc
     2281 gacgacaaac gcgccgaaca atacgacaat cagcggcaaa tcgactttct gtccgggcag
     2341 gtaggagaag gtaacggcat ccgtattaat gacggcaagc agcaggaaga gcagcaggat
     2401 aatgattttg atgacggtat agataagttt catgtttgct ccgtaaataa aaacgccgcc
     2461 cggacagctt cgcactctga accaagattg cgctagttta aacgatttgc acggtttcgg
     2521 ataggatgcg gcagcgtgtt cggacgacat acggagtatg cgattgccga caattttacc
     2581 aaatacaccg ttcctttcca tttgaaaaat aacggattgg acaccgcatc gacagaaaaa
     2641 cccgccgcgc acttgtcaaa accctgtttg caggcgtatc tttacaatct tcaaattcaa
     2701 accgttcatt gaaacatatc agaataagaa aggctttaca tcatgagcag acccgtaccc
     2761 gccgtattcg gcagcgtttt tcacagtcaa atgcccgtcc tcgcctaccg cgaaggcaaa
     2821 tggcagccga ccgaatggca atcttcccaa gacctctccc tcgcaccggg tgcgcacgcc
     2881 ctgcactaca gcagcgaatg tttcgaggga ctgaaagcct tccgtcaggc agacggcaaa
     2941 atcgtactgt tccgtccgac tgccaacatc gcgcgtatgc ggcaaagtgc ggacattttg
     3001 cacctgccgc gccccgaaac ccaagcctat ctcgacgcgc taatcgaatt ggtcaaacgc
     3061 gccgccgatg aaattcccga tgcgcctgcc gccctgtacc tgcgtccgac cttaatcggt
     3121 accgatcccg tcatcggcaa ggccggttct ccttccgaaa ccgccctgct gtatattttg
     3181 gcttcccccg tcggcgacta tttcaaagtc ggctcgcccg tcaaaatttt agtggaaacc
     3241 gaacacatcc gctgcgcccc gcatatgggc cgcgtcaaat gcggcggcaa ctacgcttcc
     3301 gccatgcact gggtgctgaa ggcgaaagcc gaatacggcg caaaccaagt cctcttctgc
     3361 ccgaacggcg acgtgcagga aaccggcgcg tccaacttta tcctgattaa cggcgatgaa
     3421 atcattacca aaccgctgac cgacgaattt ttgcacggcg taacccgcga ttccgtactg
     3481 acggttgcca aagatttggg ctataccgtc agcgaacgca atttcacggt tgacgaactc
     3541 aaagctgcgg tggaaaacgg tgcggaggcg attctgaccg gtacggcagc cgtcatctcg
     3601 cccgttactt ccttcgtcat cggcggcaaa gaaatcgaag tgaaaagcca agaacgcggc
     3661 tatgccatcc gtaaggcgat taccgacatc cagtatggtt tggcggaaga caaatacggc
     3721 tggctggttg aagtgtgctg atgctttaaa taaaaaatgc cgtctgaaac ccgtttggcg
     3781 tttcagacgg cattttcgca tccgaaccgt ttccgccgca cctgcagcaa gtcggcacaa
     3841 aggcaatcgg ttaaaacaag cgtccgcatt tcccatcccg cctgccgtaa gtcgggcatt
     3901 tccctagaaa tacgcttcag acggcaaacc gccgcccgaa accgatacgc ggtacggacg
     3961 gcgcacggat ttgaaaacgg cggattatcc ctcggtgctc aaggcattaa tgctgtaacc
     4021 gccgtcaacg taagtgattt cgccggtaat gccggacgac aggtcggaca gcaggaaggc
     4081 ggcggtattg ccgacttctt caatggtaac gttgcggcgc agcggattgt gggcggcgac
     4141 gtgtcccaaa agtttgccga aatcggcgat accggaggcg gcaagcgttt taatcgggcc
     4201 ggcggaaatg ccgttgcagc ggatgccttc tttgcccaga caggcggcgg taaaacggat
     4261 gcccgcctca aggctggctt ttgccatacc catcacgttg taatttggaa tcgcgcgcac
     4321 cgcgcccaag tagctcaggg caacgatggc ggagtttctg ccgcgcatca tcggacgggc
     4381 ggcttttgcc aatgcgggca ggctgtatgc ggaaatttcg tgtgcggtgt tgaacgcttc
     4441 gcggctgatg ctgtcgagga agtcgccgct caaggcttct ttcggcgcga agccgatgga
     4501 atgcaccaaa ccgtccaagc cgtcccaatg tttgcccaag tcggcgaaca cttggttgat
     4561 ttcgtcgtcg ctggcgacat cgcagcggaa tacgagttcg gaacccaatt ccgccgccat
     4621 tttgcggacg cgctcttcca gtttgtccac aacgtaggta aacgccagtt ccgcgccttg
     4681 ttcgcggcag gctttggcga tgccataagc gatggaacgc tcggaaatca tgccggtaat
     4741 cagaattttt ttgccttgca gaaaacccat tttgtatcct tcaaatagtg ttcggttcat
     4801 tctaaaccgt tgattatagc aaattgtccc tgtttctgtg ttttcacgtt gcagcatgca
     4861 aacggcaatg ccgtccgaag cggatttcag acggcatcgg acgtttcaaa tacggtttaa
     4921 ggcatcagat gccgcgcaac aattcgttca cgctggtttt cgcacgggtt tgcgcgtcca
     4981 cgcgtttgac gatgacggcg cagtaaaggc tgtggctgcc gtctttggaa ggcatactgc
     5041 cggacacgac gaccgaaccg gccggtacgc ggccttggta gatttcgccg gtggtgcggt
     5101 cgaagatttt ggtggattga ccgatgaaca cgcccataga aatcacgctg ccttcttcga
     5161 caatcacgcc ctcaacgatt tcggaacgcg caccgatgaa gcaattgtct tcaataatgg
     5221 tcgggctggc ttgcaggggt tcgagtacgc caccgatgcc gacaccgccg ctcaagtgga
     5281 cgtttttgcc gatttgcgcg caagagccga cggtcgccca agtatcgacc atcgcacctt
     5341 cgtcaacgta tgcgccgatg ttgacgtaag acggcatcag cacgacattt ttcgccacaa
     5401 agctgccgcg tcgggcaacc gcacccggaa cggcgcggaa gcctgcgttt ttgaactcgt
     5461 cttcagacca gtcggcaaac ttggtcggca ctttgtcgaa gtatttgttc acaccgtcgt
     5521 tgaggatttc gttgtcttgg atgcggaagg acagcaacac ggcttttttc gcccattcgt
     5581 taactttcca ttcgcccacg cccaagcgtt cggcgacgcg cagtttgccg gaatcgagtt
     5641 ggcggatggt ttccaacacg gcttctttaa cttcgggagt aacggtggtc ggattaatgt
     5701 ccgcgcggtt ttcaaaggcg gtttcgataa tgttttgcaa agacataatg tttccttatg
     5761 tgagatgttt cgtgggacgc ttgccggtat tttcaggcag tatgttcatt tgaaatgaat
     5821 gcgctattgt gccacgcaaa cgcatattcg tcagcaatac gcgggcggtt gccgaacatt
     5881 ggcgtttcgg aatcgggcag gcggcaggta ttgcctttat gccgcgccgc cgcctttcag
     5941 acggcattcg cggtaaaacg ccatcagccc ttcggtcgag gcatcgtgta cggacgtgcc
     6001 gccttccagt tcgccgatga tggttttcgc caactgtttg ccgtattcca ccccccactg
     6061 atcgaagggg ttgatgtccc aaattacacc ctgcacaaag gttttgtgtt cgtaagccgc
     6121 catcagcata cccagattgt agggcgtgag gcggtcgagc aggatgctgt tgctggggcg
     6181 gttgccgggg aactctttgt gcggcgcgag gcgttcgcgt tccgcttcgg gcaaatctgc
     6241 cagttcggcg cgtgcttcgt ccaaggtttt gcccttcatc aaggcttccg cttgggcaaa
     6301 ggcgttggca acggtaaaac ggctgcgtcc gtcctccacg ccctgcgccg tcatcgggac
     6361 gataaagtcg cagggaatca ggcgtgtgcc ttggtggagc agttggaaat aggcgtgctg
     6421 gcagttgacc ccttcaccgc cgaacacgat gccgcccgtt ttgcacgcgg cgggactgcc
     6481 gtctgaagcg cggcttttgc ccaaactctc catatcgagc tggttcagcc acgccggcag
     6541 caggcgcagg ttgtggctgt acggaacggc ggtctgcccg tccgcctgct ggaaattgtt
     6601 ataccacacg gcaatcagtg ccattaaaac ggggatatta tgacgcgtcg gcgtactgaa
     6661 aaaatgcctg tccatcgcgt gcgcccccgc caacaactcg cggaaacgcg ccccgccgac
     6721 cgcaaccatc acgggcaaac cgacgggcga ccagacggaa tagcgtccgc ccacccagtc
     6781 gtacatcgca aacacgcgtt ccgccgcgat accaaaagcc gcagctgccg cagtgtcggc
     6841 agacaccgcg caaaaatggc acgccgtttc ggattccgag aaccccgcac cgcgatacca
     6901 cgccttgact gcctgtgcat tgagcagggt ttccggtgtt ttgaaggact tgctggcaac
     6961 gcaaaacact gtcgtttcgg ggttcagacg gcataagacc gcatccaggc aggcagggtc
     7021 ggcgttggcg gcaaaatgga cggcgatatg ccgtctgaac ggctcgagtg cctgcacgca
     7081 cattgccggc ccgaggtcgg atccgcctat gccgatgtgg acaaaatccg taatccgttt
     7141 tccggttgtc ccctgatacg aaccgtcgtc caaactgtgt gcaaacttca acgcacgatt
     7201 taactcgcgg cggatttcgg gcaacacgtc cctgccgtcc gcataaacgg catccgcacc
     7261 gtcgggtagg cgcaaagccg tatgcagcgc ggcacgcccc tcgctgccgt tgactttcgc
     7321 acccgtccgc aaagcacgca ttttcccttc caaatccgcc gtctccgcaa gacggcagag
     7381 cagttgcagc gtatcttcgc ccaaacggtt tttgctgtaa tcgaacaaca tcccgtccaa
     7441 acgctcatgc atacgctcaa aacggtcggg ttcgcaggca aagcggtcgc gcaaaaggac
     7501 atgacgcgta tcctgattgt ggcgttcgag cgcataccat gcacgggtaa aagcattcat
     7561 ctgttttcct tgatttttca gaaccggatt aaaatgtagc agaatgtagt ttaacaaacg
     7621 gcagcggctt tggcgaatct ccggaacacc gcccgccgcg tccaatagca ccttatttcc
     7681 atatggcgaa aatatagtga attaacaaaa atcaggacaa ggcggcgagc cgca
//
LOCUS       DAWHSK010000077         7683 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.077, whole genome shotgun sequence.
ACCESSION   DAWHSK010000077 DAWHSK010000000
VERSION     DAWHSK010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7683
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.077"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            66..326
                     /locus_tag="WIC03_001033"
     CDS             66..326
                     /locus_tag="WIC03_001033"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883545.1"
                     /translation="MENAAASPAGHFRRHSRRRKCPTLKAQTHTQNRMPAPATIRQIP
                     LFFPNRLQVSPIRRVMPPPPFKGNARVNGFAVGKAAGYRYVS"
     gene            433..957
                     /gene="nspA"
                     /locus_tag="WIC03_001034"
     CDS             433..957
                     /gene="nspA"
                     /locus_tag="WIC03_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein NspA"
                     /protein_id="HEZ3883546.1"
                     /translation="MKKALAALIALALPAAALAEGASGFYVQADAAHAKASSSLGSAK
                     GFSPRISAGYRINDLRFAVDYTRYKNYKAPSTDFKLYSIGASAIYDFDTQSPVKPYLG
                     ARLSLNRASVDLGGSDSFSQTSTGLGVLAGVSYAVTPNVDLDAGYRYNYIGKVNTVKN
                     VRSGELSAGVRVKF"
     gene            1093..1482
                     /locus_tag="WIC03_001035"
     CDS             1093..1482
                     /locus_tag="WIC03_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEZ3883547.1"
                     /translation="MSKTIIHTDKAPAAIGAYSQAVRAGDTVYMSGQIPLDPATMTVV
                     GNGDFRAEARQVFQNLQAVAEAAGGTLADIVKLNAYLTDLGNFAVFNEVMAEFIAEPF
                     PARAAVGVASLPKGVQVEAEAVLVLNA"
     gene            1560..2390
                     /locus_tag="WIC03_001036"
     CDS             1560..2390
                     /locus_tag="WIC03_001036"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HEZ3883548.1"
                     /translation="MAHYAIGDIQGCFDELTALLGKIGFNHGTDTLWLTGDIVNRGPK
                     SLETLQFCIRHENSVQIVLGNHDLHLLAVGCGEGAPKRSDTIEPILKHPDGKKMLDWL
                     RAQPLLIREGSRVMVHAGILPQWRIAKAESLAREAEAELRGKKYVKFFSKMYGNKPAA
                     WDEGLEGYARLRFIVNAFTRMRALTFKNELDFDYKSTVKKMPPYLRPWFKAPDRQNLD
                     RTIIFGHWSSLGYTNADNVISLDTGALWGGQLTAVNLETEEITQVQAAGGIDWKSFAK
                     "
     gene            2405..3862
                     /locus_tag="WIC03_001037"
     CDS             2405..3862
                     /locus_tag="WIC03_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEZ3883549.1"
                     /translation="MHKILPIAHVLSRLGMLFSFILLIPAALSYAFSDGAYTAFATTA
                     TVTLSGSCIVRLATLRFRRELRPRDGFTLVLMLWLAFAAMAAMPMYLYFPNMGFTDAF
                     FESMSGLTTTGATVIPHVDGLAPSVNFWRHMLNWLGGMGIIVLAVAILPMLGVGGTQL
                     FKAEIPGIDKESKMSPRISQVAKKLWFGYTLITILAAACLHFAGMGWFDAVCHAMATL
                     SLGGFSTHDASIAYYNSPLIEAVIIVFTIVGGINFANHFAALNSRSLKTYWKDEECRT
                     MLLLLSGSILAAALYLWHTGYYAGFTESLRYTAFNFVSIGLANGLANTDFAQWPLLIS
                     LWMFFLANILANSGSTGGGIKTIRALVLFKFSLREMMVLLHPKAVRTVKISGKAIPDR
                     LALTVMSFIFIYFMTVVLFSFLLMASGMEFTTAFTAVIACITNAGPGLGEVGPAGNYA
                     GLDVMQKWICVTAMLLGRLEIFTVFILFTPAYWKK"
     gene            complement(3903..4292)
                     /locus_tag="WIC03_001038"
     CDS             complement(3903..4292)
                     /locus_tag="WIC03_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883550.1"
                     /translation="MWKISKENCEDLGFAIVCMFYDAINLSEFKLWLDIVVRDTPIDT
                     IPLYIFDLIDFDKSIGEIYDVIGVVNYGYISNDQKNALTGIAFLRGIDVYDPPISKEK
                     ALKALEKYPEIYQRFQHFFPFVELPLF"
     gene            complement(4474..4875)
                     /locus_tag="WIC03_001039"
     CDS             complement(4474..4875)
                     /locus_tag="WIC03_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883551.1"
                     /translation="MKIKPLQFSNNSRRFYVDKICVTEQDVNILASDRNYELNIEIFI
                     DNIIHFQITDESYKVKFSEYLFENKEKNDWDRNPAINYFFEIIDDSYMDWLKEESFDF
                     FEKKYYKAYIFFFSDSVIEVISSTEPVFYSK"
     gene            complement(4983..5162)
                     /locus_tag="WIC03_001040"
     CDS             complement(4983..5162)
                     /locus_tag="WIC03_001040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883552.1"
                     /translation="MPSETVFNFQATFPLFETGYWELSSSKILHLLLPRQHLGQNGRR
                     LKSKHCHQPFEDRME"
     gene            complement(5181..5558)
                     /locus_tag="WIC03_001041"
     CDS             complement(5181..5558)
                     /locus_tag="WIC03_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 41 family protein"
                     /protein_id="HEZ3883553.1"
                     /translation="MCEFKDFRRNIPCFKEYDENSFIGKWYDDGVWDDEEYWKLENAL
                     IEVRRKYPYPMDIPRDIVIGIGSIIEFLMVPNWKLFTIKSSPWLPKSIKINERYERFR
                     VMLRYIFTEKDIVNVRFDYYNKK"
     gene            complement(5589..5987)
                     /locus_tag="WIC03_001042"
     CDS             complement(5589..5987)
                     /locus_tag="WIC03_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 41 family protein"
                     /protein_id="HEZ3883554.1"
                     /translation="MCEFKDFRRNIPCFKEYDENSFIGKWYDDGVWDDEEYWKLENDL
                     IEVRRKYPYPMDIPRDIVIGIGSIIDFLMVQNWKLFEIKASPWLPKSVKINERYERFR
                     VMLRYIFTDLDVDDWKFFYFPIQHSKGRLR"
     gene            complement(5993..6669)
                     /locus_tag="WIC03_001043"
                     /pseudo
     CDS             complement(5993..6669)
                     /locus_tag="WIC03_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hint domain-/BECR-fold containing toxin"
     gene            complement(6803..7120)
                     /locus_tag="WIC03_001044"
     CDS             complement(6803..7120)
                     /locus_tag="WIC03_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883555.1"
                     /translation="MNILPSWLRVGMNIAMLGMIHSDIRLITVDYEEGRRFLKIKNYL
                     SREAITEDHEDMEYLITELWSMCGEYFDEADFECIYSNHSSMELNQINGAVFRRKELI
                     SQA"
     gene            complement(7123..>7683)
                     /gene="mafB"
                     /locus_tag="WIC03_001045"
     CDS             complement(7123..>7683)
                     /gene="mafB"
                     /locus_tag="WIC03_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HEZ3883556.1"
                     /translation="NPFISAGEAVDQWMQENPNAAETVEALVNVLPFAKVKNLTKAAK
                     PGKAAVSGDFSDSYKHNTASRLSQSVDGEMFQTRNVDFKAKSIGTKIHDGAQGKHISG
                     HRNYIEGKSTLNQNINPQELLNGIHSGAYPVISKGARGNPVVDFGYPIGSDGKSGLST
                     NFGTIHSGKNGVHIVPANPKTIKKVQ"
BASE COUNT         1940 a         2109 c         1541 g         2093 t
ORIGIN      
        1 cgcaaacaaa tcggcggccg cccgggaaaa cccgccccga acgcgtccgg aaaatatgct
       61 tatcgatgga aaacgcagcc gcatcccccg ccgggcattt cagacggcac agccgccgcc
      121 ggaaatgtcc gacgcttaag gcacagacgc acacacaaaa ccgtatgcct gcacctgcaa
      181 caatccgaca gataccgctg ttttttccaa accgtttgca agtttcaccc atccgccgcg
      241 tgatgccgcc gccaccattt aaaggcaacg cgcgggttaa cggctttgcc gtcggcaaag
      301 cagccggata ccgctacgta tcttgaagta ttgaaaatat tacgatgcaa aaagaaaatt
      361 taagtataat aaagcagaat tctttaacga attcttaaca atttttctaa ctgaccataa
      421 aggaaccaaa atatgaaaaa agcacttgcc gcactgattg ccctcgctct cccggccgcc
      481 gcactggcgg aaggcgcatc cggcttttac gtccaagccg atgccgcaca cgcaaaagcc
      541 tcaagctctt taggttctgc caaaggcttc agcccgcgca tctccgcagg ctaccgcatc
      601 aacgacctcc gcttcgccgt cgattacacg cgctacaaaa actataaagc cccatccacc
      661 gatttcaaac tttacagcat cggcgcgtcc gccatttacg acttcgacac ccaatcgccc
      721 gtcaaaccgt atctcggcgc gcgcttgagc ctcaaccgcg cctccgtcga cttgggcggc
      781 agcgacagct tcagccaaac ctccaccggc ctcggcgtat tggcgggcgt aagctatgcc
      841 gttaccccga atgtcgattt ggatgccggc taccgctaca actacatcgg caaagtcaac
      901 actgtcaaaa acgtccgttc cggcgaactg tccgccggtg tgcgcgtcaa attctgatat
      961 gcgccttatt ctgcaaaccg ccgagccttc ggcggttttg ttttctgcca ccgcaactac
     1021 acaagccggc ggttttgtac gataatcccg aatgctgcgg cttctgccgc cctatttttt
     1081 gaggaatccg aaatgtccaa aaccatcatc cacaccgaca aagcccccgc cgccatcggc
     1141 gcgtacagcc aagccgtccg agcaggcgac accgtttaca tgagcggtca aatccccctc
     1201 gatcccgcca cgatgaccgt cgtcggcaac ggcgatttcc gcgccgaagc gcgccaagtg
     1261 ttccaaaacc tgcaagccgt cgccgaagcg gcaggcggca cgctggccga catcgtcaaa
     1321 ctcaacgcct acctgaccga cttgggcaat tttgccgtct tcaacgaagt gatggcggaa
     1381 tttattgccg agcccttccc cgcccgcgcc gccgtcggcg ttgcctccct gccaaaaggc
     1441 gtgcaggtcg aagccgaagc cgtcctcgtt ttgaacgcat aattccacgt tcggactgca
     1501 tgatatttca aaaccatgcc gtccgaaccg caacactttc ccacttcaca acatctgcaa
     1561 tggcacatta cgcaatcggc gacatacaag gctgtttcga cgaactgacc gcgctgctcg
     1621 gcaaaatcgg tttcaaccac ggcacggaca ccctctggct gacgggcgac atcgtcaacc
     1681 gcggcccgaa atccctcgaa acgctgcaat tctgcatccg gcacgaaaac agcgtgcaaa
     1741 tcgtcctcgg caaccacgac ctgcacctgc tcgctgtcgg ctgcggcgaa ggcgcgccca
     1801 aacgcagcga cacaatcgaa cccatactca aacaccccga cggaaaaaaa atgctcgact
     1861 ggctgcgtgc gcaaccgctg ttgatacgcg agggcagccg cgtgatggta cacgccggca
     1921 tcctgccgca atggcgcatc gccaaagccg aatcgctcgc tagagaagcc gaagccgaac
     1981 tgcgcggcaa aaaatacgtc aaattcttct ccaaaatgta cggcaacaaa ccggccgcgt
     2041 gggacgaagg tttggaaggc tatgcccgcc tgcgcttcat cgtcaacgcc ttcacgcgga
     2101 tgcgcgccct gacctttaaa aacgaactgg atttcgacta caaatccaca gtgaaaaaaa
     2161 tgccgcctta cctgcgcccg tggttcaaag cccccgaccg gcaaaacctc gaccgcacca
     2221 tcatcttcgg acactggtcc tcgctgggct acacgaatgc cgacaacgtc atctcgctgg
     2281 acaccggcgc gctgtggggc ggacagctga ccgccgtcaa cctcgaaacg gaagaaatta
     2341 cccaagtcca agccgccggc ggcatagact ggaaaagctt cgcaaaataa cggaccgccc
     2401 cactatgcac aaaatcctgc ccatcgccca cgtcctctcc cgactgggta tgctgttttc
     2461 ctttatcctg ctgatacccg ccgccctctc ctacgccttt tcggacggcg cgtacaccgc
     2521 cttcgccacc accgcgaccg ttaccctttc cggctcgtgc atcgtccggc tcgccaccct
     2581 ccggttcagg cgcgaactgc gcccgcgcga cggcttcacc ctcgtcctga tgttgtggct
     2641 ggcgtttgcc gctatggcgg cgatgccgat gtacctgtat ttcccgaata tgggctttac
     2701 cgacgcattt tttgaatcga tgtcgggact gaccaccacc ggcgcgaccg tcatccccca
     2761 cgtcgacggg ctcgccccct ccgtcaactt ttggcggcat atgctcaact ggctgggcgg
     2821 gatgggcatc atcgtccttg ccgtcgccat cctgcctatg ctcggcgtag gcggcacgca
     2881 gttattcaaa gccgaaatcc ccggcattga caaagaaagc aaaatgtcgc cgcgcatttc
     2941 ccaagtggcg aaaaaacttt ggttcggcta caccctgatc accatcctcg cggcagcctg
     3001 cctgcatttt gccgggatgg gctggttcga tgccgtctgt cacgcgatgg cgaccctctc
     3061 gctgggcggg ttttccaccc acgatgccag catagcttat tacaactccc ccctcatcga
     3121 agcggtcatc atcgttttca ccattgtcgg cggcatcaat tttgccaacc atttcgccgc
     3181 cctcaacagc cgcagcctca aaacctattg gaaagacgaa gaatgccgga cgatgctgct
     3241 gctgctctcc ggcagcatcc ttgccgccgc cctgtacctg tggcacaccg gctattacgc
     3301 cggttttacc gaatccctgc gctacaccgc cttcaacttc gtctccatcg gactggcaaa
     3361 cgggctggcc aacaccgact tcgcacaatg gccgctcctg atttccctgt ggatgttttt
     3421 cctcgccaac atcctcgcca actccggctc gaccggcggc ggcatcaaaa ccatacgcgc
     3481 cctcgtcctg ttcaaattca gcctgcgcga aatgatggtg ctgctgcacc ccaaagccgt
     3541 ccgcaccgtc aaaatcagcg gcaaagccat acccgaccgc ctcgcgctga ccgttatgtc
     3601 cttcatcttc atctacttta tgaccgtcgt cctcttcagc ttcctgctga tggcgagcgg
     3661 tatggaattt accaccgcct tcaccgccgt catcgcctgc atcaccaacg ccggccccgg
     3721 cctgggcgaa gtcgggcccg ccggcaatta cgccggtttg gacgtgatgc agaaatggat
     3781 ctgcgttacc gccatgctct tgggcaggct ggaaatcttc accgtcttca tcctcttcac
     3841 ccccgcctac tggaagaaat aaaaaatgcc gtctgaaaac ttttcagacg gcatattgtc
     3901 ttttaaaaaa gcggaagctc tacaaaagga aagaaatgct gaaacctctg ataaatttca
     3961 ggatatttct ctaaggcttt taatgctttt tcttttgaaa taggcggatc atagacatct
     4021 atccccctta agaaggcaat gcccgttaat gcattttttt gatcatttga aatgtaacca
     4081 taattaacga ctccaattac atcataaatt tcccctatac tcttatcaaa atcaatcaaa
     4141 tcaaaaatat acaatggaat tgtatcaata ggagtatctc tgacaactat atccaaccat
     4201 aatttaaatt cagaaagatt aatagcatca tagaacatac agactattgc aaatcctaaa
     4261 tcttcacaat tttctttact aattttccac ataattattt acctttatcc attgcttccc
     4321 aaccgataca tccggtattt tagattctcc tttgccgtca aacgctcttt tggttacctg
     4381 ctttgtatca aaaatgccgt ctgaaagccg aataccgctt cagacggtat tttgattgtt
     4441 gggtttttaa tcagagcttt gtttgataat ttgttatttt gaataaaata caggttctgt
     4501 cgagctgata acttctatta cagaatcgct aaaaaagaaa atataagcct tataatattt
     4561 cttttcaaaa aaatcaaaac tttcttcttt caaccagtcc atataactgt catctattat
     4621 ctcgaaaaaa taatttatag caggatttct atcccaatca tttttttctt tattttcaaa
     4681 taaatattct gaaaatttta ctttataaga ttcatctgtt atttgaaaat gaattatatt
     4741 gtcaataaat atttcaatat ttaattcata atttcggtcg gatgccaaaa tgttcacatc
     4801 ttgttcggta acacaaattt tgtctacgta aaatctgcga ctattgttag aaaattgtaa
     4861 tggtttaatc ttcactatcg ttttccatat ctgatttttg ttttggtaac acgacgatcg
     4921 gggtttcagc gacaccgagg tattttaccg tctaatcgtg gagattttac cgattttctt
     4981 tcctattcca tccggtcttc aaatggttga tgacagtgtt tgcttttcag ccgtctgccg
     5041 ttctgaccaa ggtgctgccg tggaaggagc aggtgtagga tttttgaaga acttagttcc
     5101 caatatcctg tttcaaataa ggggaaggtc gcctgaaaat taaacacggt ttcagacggc
     5161 attttgattg ccggggtttg ctattttttg ttgtaataat caaatcgtac gttgactatg
     5221 tctttctcgg taaaaatata acggagcatt actctgaacc tttcataacg ctcattaatt
     5281 ttgatacttt taggcaacca aggggaagat ttaattgtaa aaagtttcca atttggaacc
     5341 attaaaaact caataatgct accgattcca atcacgatat cccttggtat atccatcgga
     5401 taaggatatt ttctcctaac ctcgattaaa gcattctcca atttccaata ttcttcatca
     5461 tcccacaccc cgtcatcata ccatttgcca ataaatgaat tttcgtcata ctccttaaaa
     5521 caagggatgt ttcttctaaa atccttgaac tcgcacataa taatgtatct ccaatataat
     5581 taaacttttc atctcaatct acctttacta tgttgtattg gaaagtaaaa aaatttccag
     5641 tcatctacat ctagatcagt aaaaatataa cggagcatta ctctgaacct ttcataacgc
     5701 tcattaattt tgacactttt aggcaaccaa ggggaagctt taatttcaaa aagtttccaa
     5761 ttttgaacca ttaaaaaatc aataatgcta cctattccaa tcacgatgtc ccttggtata
     5821 tccatcggat aaggatattt tctcctaacc tcgattaaat cattctccaa tttccaatat
     5881 tcttcatcat cccacacccc gtcatcatac catttgccaa taaatgaatt ttcgtcatac
     5941 tccttaaaac aagggatgtt tcttctaaaa tccttgaact cgcacatcat aattaatctc
     6001 caatacgatt taggttttta tcaaatgtac cgtttcttgt ttcttttctg ttcagcttct
     6061 tgggtgaaga tgcttctttc caagcccctc cattatgtga atctacatcg cgtgatatat
     6121 aactctttcc ttttttaaaa atagcagcat catttctcgt tctttctttt atttttctat
     6181 atcccaattt ctttgctgct gcatatgctt ctgaatcatt cccatatacg ggggtagatg
     6241 gtgtttttct tggcggacac gcattatgaa cccaaacccc ttccgttccg cctgattacc
     6301 cttgacgaag taggtatgcc aatcggcgac ggtcagattg taggctttga gcggtgttgg
     6361 tttgacaacg atgttgcgga cggtttgggt tttgccgctt tcggataaca gcctgcttcc
     6421 cgctttcaaa tcttccgctt taatccattt gctgtccgaa taaaacgggt ggatgcggtt
     6481 ggaaatcagg gtttggctgt taccgatgcc gtctgaaatt tcaatgtaaa cggtttcttg
     6541 atacggattg ccgtatcggg cggtaacggg tttgtatccc gtttttccgc ttgcctcgtc
     6601 cttggcgaag acgcggtcgc cggttcggat acgggcaatg gctttgtagc cgtctgccgt
     6661 tttgaccaag gtgctgccgt ggaaggagca ggtgtaggat ttttgaagaa ctcagttctc
     6721 aatatcctat ttcaaatgaa ggaaatgccg tctgaaaatt aaacacggtt tcagaagaca
     6781 ttgtgattgt tggtttttta acctacgctt gcgaaattaa ttcctttctc ctgaatactg
     6841 caccatttat ttggtttaac tccatagaag aatgattaga ataaatacat tcaaagtcag
     6901 cttcatcaaa atattctcca cacatagacc ataactctgt aatcaaatat tccatatctt
     6961 catggtcttc tgtgatggct tctcttgata aataattttt gatttttaaa aaccttcttc
     7021 cttcctcgta atctacggta attaacctga tatctgagtg tatcatgccc agcattgcta
     7081 tattcatacc gactcgcagc cagcttggta atatattcat aactattgca cctttttaat
     7141 ggttttaggg ttagccggaa caatgtgaac tccattttta cctgaatgaa tcgtaccaaa
     7201 attggtactt aatcctgatt tcccatcgct gcctatagga tacccaaaat caacaacagg
     7261 atttcctctt gctcctttag aaataactgg ataagcacct gaatgtattc cgttcaacaa
     7321 ttcttgagga ttaatgtttt gatttaaagt actcttacct tcaatgtagt ttctatgtcc
     7381 tgaaatatgt ttcccttgag ctccatcatg aattttagtc ccaatagatt ttgctttaaa
     7441 atcaacattg cgggtttgaa acatttctcc atctacagac tgagataatc ttgaagcagt
     7501 gttatgcttg taggagtctg agaaatcccc actaaccgca gccttccccg gttttgccgc
     7561 ctttgtcagg tttttgactt tggcaaacgg caggacgttg accagggctt cgacggtttc
     7621 ggcggcattg gggttttcct gcatccactg gtcaacggct tcgcccgcgc tgataaaggg
     7681 gtt
//
LOCUS       DAWHSK010000078         7679 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.078, whole genome shotgun sequence.
ACCESSION   DAWHSK010000078 DAWHSK010000000
VERSION     DAWHSK010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7679
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.078"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..207)
                     /locus_tag="WIC03_001046"
     CDS             complement(<1..207)
                     /locus_tag="WIC03_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HEZ3883557.1"
                     /translation="MIERLKAVENQAEAMERGARSAYLKQQAQELGISLATLYRKLEA
                     VSVKPTRKRRSDAGKTELKPEEAKL"
     gene            complement(200..1075)
                     /locus_tag="WIC03_001047"
     CDS             complement(200..1075)
                     /locus_tag="WIC03_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3102 domain-containing protein"
                     /protein_id="HEZ3883558.1"
                     /translation="MSNEVEVMDAVSVQNYQVAHSVMVMEQWGNGEVYNEATWIERGR
                     LAVRQTMEGMFELGRALIVLKEHTEHGRFMEIVKGQFGIGHNETARLMSATQRFATPQ
                     MQKAAPKLMDLGKSKLLELLVEEDVTLVGLAEGEEVNGMTFDDVDRMTVRELRVALRE
                     SRENLAAKDEVMKTKTAKIDELTEKLAKKQTVVREPKAEDVGSELAMQLTSLEIGIRS
                     QVSRLKDLFDQLNAHSEAHEISHQAKMVGTLNQIILDCEQLRESYALPTEAPTDNVPE
                     WLGGETGEGDESGND"
     gene            complement(1121..1411)
                     /locus_tag="WIC03_001048"
     CDS             complement(1121..1411)
                     /locus_tag="WIC03_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEZ3883559.1"
                     /translation="MAESKRVQRLLRVFIALDEHPIIGLSNKDLSVGLGLTPSQVSRD
                     IDDLVASGLVIKLENGNYAYGIKTLQIAERFRRQHERLQSKIAEIGKRVNAD"
     gene            complement(1423..1668)
                     /locus_tag="WIC03_001049"
     CDS             complement(1423..1668)
                     /locus_tag="WIC03_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEZ3883560.1"
                     /translation="MINHECINQPYGRILKFSRVQAKEMLTADELKENFAQNGQTLAQ
                     WARENGFKPRDVYLVIGGQRKGNYGKGYEIAKKLGLK"
     gene            complement(1661..1885)
                     /locus_tag="WIC03_001050"
     CDS             complement(1661..1885)
                     /locus_tag="WIC03_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEZ3883561.1"
                     /translation="MEKPLNFKPIPYPQTRESAAGWFKRNGVCKAHWAKYFNLERTTV
                     EHLLRGKLKGNFGKSHEAAVLLGMKEKSDD"
     gene            2157..2528
                     /locus_tag="WIC03_001051"
     CDS             2157..2528
                     /locus_tag="WIC03_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ3883562.1"
                     /translation="MNNSSFFGNRLKEERKKLKMTQAEIAEKCGVSGRMWGDYERGIS
                     QPKAELFFQFEKVGIDVQYVMHGRRGETAVMPSETLNAEEQELLALFREAAAADREMI
                     LMVARRAEKKAQTALGKVSNG"
     gene            2530..3153
                     /locus_tag="WIC03_001052"
     CDS             2530..3153
                     /locus_tag="WIC03_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883563.1"
                     /translation="MNQFELCQKEHVNPFALSKQYLLVVTFVKSSSKNFQAALLWARS
                     AKLFENLEIGKETIYCCAFDKTAEQAGMAGVFLNYIENWNGKQIYINGRIHSGSIYDL
                     LGVLDCYQKSQSCPNPKSHCCFVSDDIFLWHGSRPTFEISLDLTGKKKETSSAKKFVM
                     PCINFRHHRIEKETYLGNWNEQIAALAVKQNIDWCPSFDIENFRQYE"
     gene            3146..3340
                     /locus_tag="WIC03_001053"
     CDS             3146..3340
                     /locus_tag="WIC03_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ3883564.1"
                     /translation="MNNYYLYRNCSSDVLWVKRIQRQIDGSLLLISDNSTYPPMPLAL
                     AEHPDIQIIGQVVQVSKDLN"
     gene            3364..3924
                     /locus_tag="WIC03_001054"
     CDS             3364..3924
                     /locus_tag="WIC03_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883565.1"
                     /translation="MKILALLIAATCALSACGGQAEEQPASAQPQEQAQSELKTMPVS
                     YTDYQSVANKGLADQKTGLTLPEHVVPADNAEGKNLLHDFSDGLTLTVDTDKADKITA
                     VRVVWNTDAMPQKAEKLSKAAAALIAATAPGDRTLLRDTGDQIKMAIDSHNAQKEPTR
                     EWARGGIAYKVTVTNLPSVVLTAKAE"
     gene            4065..5123
                     /locus_tag="WIC03_001055"
     CDS             4065..5123
                     /locus_tag="WIC03_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883566.1"
                     /translation="MKNKLTLRYFKITRCGYWSGSSKEPNHYEDLLKTLTHLEIYVKG
                     KTIEETGISRDDKEPSTFLLDICKKNGLWLLAFWNPAAESSSDEIVSLNMKSKLGQVD
                     SSPVTAGNGKTFGFVSYFLIWPEHNFYSTILSPNATLAGRTEFEEYMLNFLWIDPNFI
                     EKTTYEETSTDGIIHTMERTNFKLPLKINGTPVPRFAGKLAKSPSEKEYVISNYDQVT
                     QVHNQCTVKVTSGQSSSGILKKMLPSFFGSTELFAPLKTIRVNHSVSAAFENKSNLKD
                     WIEDWEKNSFDLDKDDCGFKLKNDDKIYWIRGNVIKCELDVNLHKENGIYPAAKILDI
                     IDQRKDYVLNEFKLSDDS"
     gene            5113..5619
                     /locus_tag="WIC03_001056"
     CDS             5113..5619
                     /locus_tag="WIC03_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883567.1"
                     /translation="MILRNIFLICSAFMIGVAWYLGANIRFEEQWVLYEALRTTASII
                     FAVVGVWLAIVYPERLKSPFSQTFSSPVYRRGFKQLFSPVISSIFILSIVLLVGLLAP
                     LLKHIEAIIQYIPLCRKISFSLLASLTMWQIYTVLSVLVPLLNILSKNENDCHASNII
                     NKLKGEQN"
     gene            5723..6226
                     /locus_tag="WIC03_001057"
     CDS             5723..6226
                     /locus_tag="WIC03_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 108 protein"
                     /protein_id="HEZ3883568.1"
                     /translation="MSTKFNQFINRVLSHEGGYVSHPKDPGGETNWGITKRTAQANGY
                     NGSMRAMTREQAIGIYRKAFWERYRADQMPEAVAFQFFDACVNHGYGNAVRMLQRAAG
                     VADDGIIGEISLKAINSLPENDLLLRFNAERLVFYTKLSTFTSFGKGWVRRVAQNLIH
                     ASADNTD"
     gene            6338..6670
                     /locus_tag="WIC03_001058"
                     /pseudo
     CDS             6338..6670
                     /locus_tag="WIC03_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229389.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6667..7077
                     /locus_tag="WIC03_001059"
     CDS             6667..7077
                     /locus_tag="WIC03_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase"
                     /protein_id="HEZ3883569.1"
                     /translation="MIEFVRAKKRLLWAFVLLLVWTCGYRYAADKAEAKQTALIATYR
                     HSSMVAAEQYALQLKKAQDERQRWYDFSQKQGRKPVKKQYPPQTKKAGYLKTKEELLA
                     ELACLKAEMAALKKLDALIYGKEVRQKERNSSQG"
     gene            7082..>7679
                     /locus_tag="WIC03_001060"
     CDS             7082..>7679
                     /locus_tag="WIC03_001060"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEZ3883570.1"
                     /translation="MPSVELLLVIVGLPRGTFYYQLAVQSAEDKYADLKRHIHDIYRR
                     HKGRYGYRRIAAAIRHAGTPVNHKKVSRLMAKTGLKAVIRRRKYRSFKGEVGKIAPNI
                     LRRCFHAEKPNEKWVTDVTEFNVGGEKIYLSPIMDLFNGEIVSYRIQTRPTFDLADEI
                     LKGAPEKPGSSEKPILHSDQGWQYQMFFYSGLNLNQDKA"
BASE COUNT         2106 a         1778 c         1722 g         2073 t
ORIGIN      
        1 caatttggct tcttccggtt taagctccgt cttgcccgca tcgctgcgcc gtttgcgcgt
       61 cggcttgacg ctgaccgcct ccagcttgcg gtatagcgtg gcaaggctga tgcccaattc
      121 ctgcgcctgc tgcttaagat atgcagagcg tgcgccgcgt tccattgctt ccgcctgatt
      181 ctcgactgcc ttaagacgct caatcattgc cggattcatc gccttctccc gtttcaccgc
      241 ccaaccattc cggcacattg tctgtcggtg cttcagtcgg cagggcatag ctttcgcgca
      301 gttgctcgca gtccaaaata atttgattga gcgtgccgac catctttgcc tgatggctga
      361 tctcgtgtgc ctcactgtgc gcattaagtt ggtcgaacaa atctttcaaa cggctcactt
      421 gactgcggat accgatctca aggcttgtta actgcatcgc caactcactg cccacgtctt
      481 ccgccttcgg ctctctgaca acggtttgct tcttagccag cttctcggtc agctcatcaa
      541 ttttggctgt tttggttttc atcacttcgt ctttggcggc gaggttttca cggctttcgc
      601 gcagggcgac gcgcagctcg cgcaccgtca ttcggtccac atcgtcaaag gtcatgccgt
      661 tgacttcttc tccttcggca agtcccacca acgtaacgtc ttcttcgacc aagagttcca
      721 gcagctttga cttgcccaaa tccatcagct tcggcgcggc tttctgcatt tgcggcgtgg
      781 cgaaacgttg ggtagcagac atcaatcggg cggtttcgtt atggccgata ccaaattgac
      841 cttttacaat ttccataaat cgcccgtgtt ctgtatgctc tttcagtaca ataagtgcgc
      901 gccccaattc aaacatgcct tccatcgtct gccgtaccgc caagcggccg cgttcgatcc
      961 aagtggcttc gttgtaaacc tcgccgttac cccattgctc catcaccatc acgctgtgcg
     1021 cgacttggta gttttgcact gaaaccgcat ccatcacttc aacttcattg ctcatttttc
     1081 actctccaaa tttccttacg tcaggaaatt tcaaaattcg ttaatctgca ttcacccgct
     1141 tgccgatttc ggcaatcttg ctttgcagcc gttcatgctg ccgcctgaac cgctcggcga
     1201 tttgcagggt tttgataccg taggcgtagt tgccgttttc aagcttgatg accaatcccg
     1261 atgcaaccaa atcatcaata tccctgctga cttgtgatgg cgtcagcccc agtccgaccg
     1321 ataaatcctt attgctcaga ccgataatcg gatgttcgtc aagcgcgata aagaccctca
     1381 atagccgttg taccctttta ctttctgcca tccgcatcct ccttatttca gtcccagctt
     1441 cttggcaatt tcgtacccct tgccgtaatt gcctttgcgc tgtccgccga tcactagata
     1501 cacatcgcgc ggcttaaagc cgttctctct tgcccactgc gccagcgtct ggccgttttg
     1561 ggcaaaattt tctttcaatt cgtctgccgt aagcatttct ttggcctgta cgcgggaaaa
     1621 tttcagtatc cgtccgtacg gttggttaat acactcatga ttaatcatcg cttttctcct
     1681 tcatccccag caaaaccgcc gcttcgtggc ttttgccaaa attgcctttc agcttgccgc
     1741 gcaagaggtg ctccaccgtg gtgcgctcta gattgaaata tttcgcccaa tgcgccttgc
     1801 acaccccgtt gcgcttaaac cacccggccg cgctctcgcg cgtttgcgga tagggaatag
     1861 gcttgaaatt caaaggtttt tccatattat ttatgccttt cgtgtgatat aatgtgtttg
     1921 tttttaggtc tctttgcacg ataaacatcc ggtctgttgt gcagcagcag ggtcttggct
     1981 tgttttaaag taaacaagct ttttctcgta gctgggtgta tgtgatccat catgagctgt
     2041 cgcgcatgag ctttcaaagt gttcattttt tcgtcctttc tcgtgatgat ttagggtgtt
     2101 tgtgtttcga tgtggaaatt ataggaagaa ttcttcctat tttgcaagga atatttatga
     2161 ataactcttc cttttttggc aaccgattga aagaagaaag aaaaaaatta aaaatgactc
     2221 aagctgaaat cgctgaaaaa tgtggggttt caggaagaat gtggggggat tatgaacgtg
     2281 gcatcagcca gccaaaagcg gaacttttct tccaatttga aaaggtgggt atagacgttc
     2341 aatacgtcat gcacggcaga cgcggcgaaa cagcggtcat gccgtctgaa accctgaacg
     2401 ccgaagaaca agaactgctt gccctgttcc gcgaggcggc agctgccgac cgtgaaatga
     2461 ttctgatggt tgcgcgcagg gcagagaaaa aagcccaaac tgcgcttggt aaagtgagta
     2521 atggataaaa tgaatcaatt cgaattgtgc caaaaagaac atgtcaatcc atttgccttg
     2581 tcaaagcaat atctattggt tgtaacattc gtcaaatcca gcagtaaaaa ttttcaggca
     2641 gcattacttt gggcaagaag tgccaaatta tttgagaatc ttgagattgg aaaagaaacc
     2701 atctattgct gcgctttcga taaaacagca gaacaggctg ggatggccgg ggtatttttg
     2761 aattatattg aaaattggaa tggcaaacag atttacatca atggccgaat ccatagtggc
     2821 agtatttatg atttgttagg ggttttagac tgctatcaaa aatcacagtc ctgtcccaac
     2881 cctaaaagcc actgttgctt tgtttcagac gacatttttc tatggcatgg atcaagacca
     2941 acgtttgaaa tcagtcttga tctaactgga aagaaaaaag aaacatcctc tgcaaagaaa
     3001 tttgtgatgc cttgtattaa tttccgtcac cataggattg aaaaagaaac ctacttagga
     3061 aattggaatg aacaaattgc cgcattggca gtaaaacaaa atatagattg gtgtccaagt
     3121 tttgatattg agaattttag acagtatgaa taattactat ctatatagga attgcagcag
     3181 cgatgtgtta tgggtcaaac gtatccaacg ccaaatcgac ggcagcctac tcttgatttc
     3241 tgacaattca acctatccac ccatgccctt ggcactggcg gaacaccccg atattcaaat
     3301 catcgggcag gtagtgcagg tatcaaaaga cttgaactag acacaatcaa aaagggaaat
     3361 agaatgaaaa tactcgcttt attaattgcc gctacctgtg ctttatctgc gtgtggcggc
     3421 caagctgaag aacaaccggc atctgcccaa cctcaagagc aggcacaatc cgaattaaaa
     3481 accatgccgg taagctatac cgactatcaa tcagtagcca ataaagggct tgccgaccaa
     3541 aaaactggtc tgacccttcc agaacatgtt gttcctgccg acaatgcgga agggaagaat
     3601 ctgctgcatg acttttcaga cggcctcaca ttaaccgttg ataccgataa agccgacaaa
     3661 attactgctg tccgagtagt ctggaataca gatgcaatgc ctcaaaaagc ggaaaaactg
     3721 tccaaagctg ccgcagcctt gattgcggca accgccccgg gagaccgcac actgctgcgt
     3781 gataccggcg accaaatcaa aatggcaatt gacagccata atgcgcaaaa agagccaacc
     3841 cgagaatggg cgcgtggtgg gattgcttat aaagtcactg ttactaattt gccgagcgtg
     3901 gttttgacag caaaagctga ataacctgca ttacagccac aattaggagc agacggagat
     3961 gatgtttata gagcctgctg agaataaata actgataaaa aagctgtctg aaaaggtttc
     4021 ggatagttag gatagctatc tatttttaat atattagaga gaatatgaaa aataaactga
     4081 ctttacgtta tttcaaaata actcgttgcg gttattggtc tggttcttca aaagaaccta
     4141 atcattatga ggacttatta aaaactttaa ctcatttaga gatatatgtt aaaggtaaaa
     4201 ctattgaaga gactggaatt agtcgtgatg ataaagagcc aagcactttc ttgctcgata
     4261 tctgcaaaaa aaatggtctg tggttgcttg ctttttggaa tcctgccgca gaaagctctt
     4321 cagatgaaat tgtttcttta aacatgaaaa gtaaactagg tcaagttgat tctagcccag
     4381 taacagcagg aaatgggaaa acttttggct tcgttagtta ttttcttatt tggccagaac
     4441 ataattttta ctcaacgatt ctttctccta atgcaacttt agctggacga acagagtttg
     4501 aagagtacat gcttaatttc ttgtggattg atcctaattt tatagaaaag actacctacg
     4561 aagaaacttc aacagacgga ataattcata ccatggagcg aactaatttt aaattgcctc
     4621 ttaaaattaa tggaactcct gtgccacgtt ttgcggggaa actagctaaa tctccttcag
     4681 aaaaagagta tgtcattagt aattatgacc aagtaaccca agtgcataat caatgcacgg
     4741 taaaggttac ctctggacaa agttcaagtg gaattttgaa gaaaatgtta ccaagctttt
     4801 ttggttcaac agaattgttt gctccattaa aaaccattcg tgtaaatcat tctgtttctg
     4861 ctgcatttga aaacaaatca aatcttaagg attggattga agactgggaa aaaaattctt
     4921 ttgatttaga taaagatgat tgtggcttta agttaaaaaa cgatgacaag atttattgga
     4981 ttcgaggaaa tgtcatcaaa tgtgaattag acgtcaacct tcataaggaa aatgggattt
     5041 atccagctgc taaaattcta gatataatag accaaaggaa agattacgtt ttaaacgaat
     5101 tcaaattaag tgatgattct tagaaatatt ttcctaatat gctctgcatt catgattggt
     5161 gtagcctggt atttaggtgc taatatccga tttgaagagc aatgggtatt gtatgaagcc
     5221 ttgagaacaa ctgcatcaat tatttttgca gttgttgggg tttggttggc tattgtctat
     5281 cccgaacgat taaaaagccc ttttagtcaa actttctcct ctccggtata tcggcgtgga
     5341 ttcaaacagc ttttttcccc tgttataagt tccattttta ttttaagtat tgtgttactt
     5401 gttggtttgt tggctccttt gctcaaacat attgaggcaa taattcaata tatcccactt
     5461 tgtcggaaaa tctcatttag cctgttagca agcttgacga tgtggcaaat ttatacggtt
     5521 ttatctgtat tagtgccttt gttaaatatt ttaagtaaaa atgaaaatga ctgtcatgca
     5581 tccaacatta ttaacaagct aaagggtgaa cagaattagc tatgtgattt ttagtgtaaa
     5641 ctgcttttaa ctcgtattaa aaggccactc ttttaatacc tcctaaaatc cctgtaccta
     5701 tcaatagata cagggatttt ttatgtccac caaattcaac caattcatca accgcgttct
     5761 ctcccacgag ggcggttacg tcagccatcc caaagacccc ggcggcgaaa ccaattgggg
     5821 catcactaag cgcaccgccc aggcaaacgg ctacaacggc tccatgcgtg ccatgacgcg
     5881 tgaacaggct atcggcattt accgaaaggc gttttgggag cgttaccgcg ccgaccaaat
     5941 gccggaagcg gtcgcgttcc aattttttga tgcctgcgtc aaccacggtt acggcaatgc
     6001 cgtccgtatg ctgcaacgcg ccgcaggcgt ggcggacgat ggaatcatcg gcgaaatcag
     6061 cctcaaagcc atcaattccc tccccgaaaa cgacctttta ttgcggttca acgccgagcg
     6121 tctggtcttt tataccaagc tcagtacgtt cacctctttc ggcaagggct gggtacgccg
     6181 tgtggcgcaa aacctgattc acgcgtctgc agataacact gattaaaggg agataaacca
     6241 tgtcaaaaaa gtcactcatc gccctaatga ccgcagccat gcagcccgat ttcagccaca
     6301 gcgacctagg cattcgctac gccatgccga ctcagggatg ttggacgcaa gcccaccgca
     6361 agagcggggt agccgccgcg aaacgcgcag ccaaaaaaaa gcgtcacaaa taaccgcctt
     6421 tttccgatgg ctgggcggct tggtctctaa tccggccaca ggaaaaatca gccataccaa
     6481 actatgggca aacgtcgccg cagccgccat gacttggaag ttcgtgcagg cggcggacgc
     6541 gcccgaatgg ctctagtggg cttatggcgc attggtcggc gggtatgcat taatcaaacg
     6601 cggcatcgcg gcgattccgc agttggcaga aatcaaaaaa tccgcaaatc aggaaggggg
     6661 gcggcaatga ttgaatttgt ccgagccaaa aaacggctgc tttgggcatt tgtgcttttg
     6721 cttgtgtgga cgtgcggtta ccgatacgcc gccgacaagg ccgaagcgaa acaaaccgcc
     6781 ctgattgcca cctatcggca ttcttctatg gttgcggcgg aacaatacgc cttgcagctt
     6841 aaaaaagcgc aggacgaaag gcagcggtgg tacgactttt cccaaaaaca aggaagaaag
     6901 cccgtgaaaa aacagtatcc gccgcaaacg aaaaaagccg gctatctgaa aaccaaggaa
     6961 gaactgcttg cggaattggc ttgccttaaa gcggaaatgg ctgccctaaa aaagctcgat
     7021 gccttaatct atgggaaaga agtgcggcag aaagaacgca actcgtcgca gggttaaggc
     7081 aatgccatcc gttgaactgc tgttggtgat tgtcggactg ccacgcggca ccttctatta
     7141 ccaattggcc gtccaatcgg cagaagacaa atatgccgat ttgaaacggc atatccatga
     7201 tatttatcga cgacataagg gaagatacgg ctaccggagg attgcggcag ccatccgtca
     7261 cgcaggaaca ccggtcaatc acaagaaagt cagccgtctg atggcgaaga cggggctgaa
     7321 ggcagtgata cggcggcgca aataccgctc gttcaaagga gaagtcggca aaattgcgcc
     7381 gaatatcctg cgacgctgtt tccatgcaga aaagccgaat gagaaatggg taacggacgt
     7441 taccgagttc aatgtaggcg gagaaaagat atacctttct ccgattatgg atttgtttaa
     7501 cggggaaatc gtcagttacc gtattcaaac ccgcccgact ttcgatttgg ccgacgagat
     7561 actgaaaggt gcgccggaga aaccgggatc gtctgaaaag ccgatactgc attcggatca
     7621 aggttggcaa tatcagatgt ttttttatag tggattaaat ttaaatcagg acaaggcga
//
LOCUS       DAWHSK010000079         7648 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.079, whole genome shotgun sequence.
ACCESSION   DAWHSK010000079 DAWHSK010000000
VERSION     DAWHSK010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7648
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.079"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..748)
                     /gene="gshA"
                     /locus_tag="WIC03_001061"
     CDS             complement(<1..748)
                     /gene="gshA"
                     /locus_tag="WIC03_001061"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEZ3883571.1"
                     /translation="MKLPVMSPEHSAQLQAFEAKILSNHAKIEAWFRTQWSVHRPPFY
                     GSVDIRNAGYKISSIDMNLFPGGFNNLNPNFIPLAAVAAQDAVQRACETAKSVLIIPE
                     NHTRNTFYLQNVYALGEILRSAGYEVRLGSLNPEVTEPTEFETALGDKILLEPLLRTR
                     DRVHLADGFSPCVVLLNNDLSAGIPDILKGISQTVLPPLHGGWTTRRKTNHFGAYNQV
                     TAEFAKLIGIDEWQINPYFEKIGGLDFQGRE"
     gene            1008..2417
                     /gene="leuC"
                     /locus_tag="WIC03_001062"
     CDS             1008..2417
                     /gene="leuC"
                     /locus_tag="WIC03_001062"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEZ3883572.1"
                     /translation="MTAQTLYDKLWNSHVVREEEDGTVLLYIDRHLVHEVTSPQAFEG
                     LKMAGRKLWRIDSVVSTADHNTPTGDWDKGIQDPISKLQVDTLDKNIKEFGALAYFPF
                     MDKGQGIVHVMGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHTMATQCI
                     TAKKSKSMLISVDGKLKAGVTAKDVALYIIGQIGTAGGTGYAIEFGGEAIRSLSMESR
                     MTLCNMAIEAGARSGMVAVDQTTIDYVKDKPFAPEGEAWDKAVEYWRTLVSDEGAVFD
                     KEYRFNAEDIEPQVTWGTSPEMVLDISSKVPNPAEETDPVKRSGMERALEYMGLEAGT
                     PLNEIPVDIVFIGSCTNSRIEDLREAAAIAKDRKKAANVQRVLIVPGSGLVKEQAEKE
                     GLDKIFIEAGFEWREPGCSMCLAMNADRLTPGQRCASTSNRNFEGRQGNGGRTHLVSP
                     AMAAAAAVTGRFTDIRMMA"
     gene            2514..2768
                     /locus_tag="WIC03_001063"
     CDS             2514..2768
                     /locus_tag="WIC03_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883573.1"
                     /translation="MNKLFITALSALALSACAGTWEGAKQDTARNLDKTQAAAERAAE
                     QTGNAVEKGWDKTKEAVKKGGNAVGRGISHLGGKIENATE"
     gene            2830..3471
                     /gene="leuD"
                     /locus_tag="WIC03_001064"
     CDS             2830..3471
                     /gene="leuD"
                     /locus_tag="WIC03_001064"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEZ3883574.1"
                     /translation="MKAFTKITAIVAPLDRSNVDTDAIIPKQFLKSIKRSGFGPNAFD
                     EWRYLDHGEPGMDNSKRPLNPDFSLNQPRYQGAQILLTRKNFGCGSSREHAPWALDDY
                     GFRAVIAPSFADIFFNNCYKNGLLPIVLTEEQVDRLFKEVEANEGYRLSIDLAGQTLT
                     TPGGETLAFDITEHRKHCLLNGLDEIGLTLQHADEIHAFEEKRRQSQPWLFNG"
     gene            3640..4911
                     /locus_tag="WIC03_001065"
     CDS             3640..4911
                     /locus_tag="WIC03_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEZ3883575.1"
                     /translation="MQQIKFIDLFSGMSGIRKGFEQACRKQSVACECVFTSEIKPAAL
                     EVLKQNYPDEVPYGDITKIETGDIPDFDILLAGFPCQAFSFAGKRLGFEDTRGTLFFD
                     VARILKAKKPKGFILENVEGLVTHDRKDPTQKIGRTLTVILETLETLGYYVSWKVLNA
                     KDFGIPQNRKRIYLTGSLKSKPDLSFETTLSPKLKNILESGLPTESSPFIKKLLKKFP
                     PSELYGKSVKDKRGGKNNIHSWDIELKGTVTEEEKQLLNMLLKERRKKKWASEIGIDW
                     MDGMPLTKAQISTFYKHPNLQNILDSLTDKGYLVLEHPKQKIGGRRIKDESLPKGYNI
                     VSGKKSFEINKILDPNDVAPTLVAMDMEHLFVVDNGGLRTLTGKEGLRLFGYPDDYSF
                     DIPKKDKYDLLGNTVAVPVIKAVSERLLHTL"
     gene            4927..5658
                     /locus_tag="WIC03_001066"
     CDS             4927..5658
                     /locus_tag="WIC03_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NgoBV family restriction endonuclease"
                     /protein_id="HEZ3883576.1"
                     /translation="MVKLTAQQIFDKLLNEEKILSVNGQIRFFLGDVDIIVKQKDVVG
                     NIIQEWLGGWLRKREIEFDVSTNTQMPPDFFLNKKDRSRELLEVKAFNRNASPGFDIA
                     DFKMYSDEIIHKPYMLDVDYLIFGYDMDDNGNVTIKDLWLKKVWQITRSMDGWAINLQ
                     VKKGVVHKIRPGVWYSINKKNMPMFECLEDFVSAIEETVYQNPATRHNASLWKKKFEE
                     AYKKHYNRSISIPRWHEIAHKYKKK"
     gene            5692..6762
                     /gene="leuB"
                     /locus_tag="WIC03_001067"
     CDS             5692..6762
                     /gene="leuB"
                     /locus_tag="WIC03_001067"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEZ3883577.1"
                     /translation="MTKHIAILRGDGIGPEIVAETVRVLDKLIAQGLDAGYEYAPLGG
                     EAYDEYGHPYPEFTQNLCRKADAVLLGAVGSPQYDNLDRPLRPERGLLAIRKDLNLFA
                     NLRPAVLYPELANASTLKPEIVAGLDILIVRELTGDIYFGEPRGIRVLENGEREGYNT
                     MKYSESEIRRIAHVAFQSAQKRSKKVCSVGKANVLETTELWREIFEEIGKEYPDVELS
                     HMYVDNAAMQLVRAPKQFDVIATGNIFGDILSDEASMLTGSIGMLPSASLDENGKGLY
                     EPSHGSAPDIAGQNKANPLATILSLAMLLRYSLNDEARAQQVENAVQKVLQQGLRTSD
                     IYEEGTKLVSCSEMGDAVLAAL"
     gene            6925..7488
                     /locus_tag="WIC03_001068"
     CDS             6925..7488
                     /locus_tag="WIC03_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenoid-binding protein"
                     /protein_id="HEZ3883578.1"
                     /translation="MKKIIFAALAAAAISTASAATYKVDEYHANARFAIDHFNTSTNV
                     GGFYGLTGSVEFDQAKRDGKIDITIPIANLQSGSQHFTDHLKSADIFDAAQYPDIRFV
                     STKFNFNGKKLVSVDGNLTMHGKTAPVKLKAEKFNCYQSPMEKTEVCGGDFSTTIDRT
                     KWGMDYLVNVGMTKSVRIDIQIEAAKQ"
BASE COUNT         2190 a         2033 c         1743 g         1682 t
ORIGIN      
        1 cttcacgccc ttggaagtcc aaaccgccga ttttttcaaa ataagggtta atttgccatt
       61 cgtcgatgcc gattaacttg gcaaattcgg cggtaacttg gttgtacgcg ccgaaatgat
      121 ttgttttgcg gcgcgtcgtc caaccgccgt gcaacggcgg caaaacggtt tggctgatgc
      181 ctttgaggat gtcgggaatg ccggcggaca aatcgttgtt caacaaaacc acgcaaggcg
      241 aaaagccgtc tgcaagatgg acgcgatcgc gggtacgcaa taaaggttcc aacaggattt
      301 tgtcgcccaa tgcggtttca aattcggtcg gttcggttac ttccggattc aggctgccca
      361 agcgcacttc ataccctgcc gaacgcaaaa tctcgccgag ggcgtaaacg ttttgcaggt
      421 aaaacgtatt gcgcgtgtgg ttttcaggaa taatcaatac ggatttcgcc gtttcgcagg
      481 cgcgttgcac cgcatcttgc gcggcaaccg ccgccagcgg gataaagttg ggattcagat
      541 tattgaagcc gccggggaac aaattcatat cgatagacga aattttgtaa ccggcattgc
      601 gtatatcgac cgaaccgtaa aacggcgggc ggtgtacgct ccactgcgtg cggaaccacg
      661 cctcgatttt ggcgtgattg gacaggattt ttgcctcaaa cgcctgaagt tgcgccgaat
      721 gttcgggcga catcaccggt aatttcatct ttcgtctgcc tctgtgtgtt ttttgacaag
      781 atgcagatga taatgccttt gaaagaatga cgacaagtat tctttcgtat cgaatcatgc
      841 tttagatata actgcttact gaaatggaca atatgtctaa tttttttgtt aaaattgttt
      901 acaaaataac accgactcaa aaattagaca aaatctgttg cgcggtataa agaatacgtc
      961 taaaatccgc ccaatccgca ttaccattta tccaaaagaa caacatcatg acagcacaaa
     1021 ccctctacga caaactttgg aacagccacg tcgtccgcga agaagaagac ggcaccgtcc
     1081 tgctctacat cgaccgccat ttggtgcacg aagttaccag ccctcaggca tttgaaggct
     1141 tgaaaatggc ggggcgcaag ctgtggcgca tcgacagcgt cgtctccacc gccgaccaca
     1201 acaccccgac cggcgattgg gacaaaggca tccaagaccc gatttccaag ctgcaagtcg
     1261 atactttgga caaaaacatt aaagagtttg gcgcactcgc ctattttccg tttatggaca
     1321 aaggtcaggg catcgtacac gttatgggcc ccgaacaagg cgcgaccctg cccggtatga
     1381 ccgtcgtctg cggcgactcg cacacttcca cccacggcgc attcggcgca ctggcgcacg
     1441 gcatcggcac ttccgaagtc gagcacacca tggcgaccca atgtattacc gcgaaaaaat
     1501 ccaaatccat gctgatttcc gttgacggca aattaaaagc gggcgttacc gccaaagacg
     1561 tggcgctcta catcatcggg caaatcggca cggcaggcgg tacaggctac gccatcgagt
     1621 ttggcggcga agccatccgc agcctttcta tggaaagccg catgacttta tgcaatatgg
     1681 cgattgaggc aggcgcgcgc tcaggcatgg ttgccgtcga ccaaaccacc atcgactacg
     1741 taaaagataa acccttcgca cccgaaggcg aagcgtggga caaagccgtc gagtactggc
     1801 gtacgctggt gtctgacgaa ggtgcggtat tcgacaaaga ataccgtttc aacgccgaag
     1861 acatcgaacc gcaagtcact tggggtacct cgcctgaaat ggttttagac atcagcagca
     1921 aagtgccgaa tcctgccgaa gaaaccgatc cggtcaaacg cagcggtatg gaacgcgccc
     1981 ttgaatacat gggcttggaa gccggtacgc cattaaacga aatccccgtc gacatcgtat
     2041 tcatcggctc ttgcaccaac agccgcatcg aagacttgcg cgaagccgcc gccatcgcca
     2101 aagaccgcaa aaaagccgcc aacgtacagc gcgtgttaat cgtccccggc tccggtttgg
     2161 ttaaagaaca agccgaaaaa gaaggcttgg acaaaatttt catcgaagcc ggttttgaat
     2221 ggcgcgaacc gggctgttcg atgtgtctcg ccatgaacgc cgaccgcctg accccggggc
     2281 aacgctgcgc ctccacctcc aaccgtaact ttgaaggccg tcaaggcaac ggcggacgta
     2341 cccacctcgt cagccccgct atggcagcag cagccgccgt taccggccgc tttaccgaca
     2401 tccgcatgat ggcgtaagta ttttcaggcg gtctttaaga atttgacgcg gccgtctgaa
     2461 atatgtcccg taaggcagaa acacctttaa tattcaaacc aaggagttta accatgaaca
     2521 aacttttcat taccgccctg tccgccctcg ccttgtccgc ctgcgccggc acttgggagg
     2581 gcgcgaaaca agacaccgcc cgcaaccttg acaaaacaca ggccgccgcc gaacgcgccg
     2641 ccgaacaaac aggcaacgcc gtcgaaaaag gctgggacaa aaccaaagaa gccgtcaaaa
     2701 aaggcggcaa tgccgtcgga cgcggcattt cccatctcgg cggaaaaatc gaaaacgcca
     2761 ccgaataacc gattcgcgat gccgtctgaa accgcttcag acggcatcgt cagcacaaga
     2821 cgcaaaccca tgaaagcctt taccaaaatt accgccatcg tcgccccgct cgaccgcagc
     2881 aacgtcgata ccgacgccat catccccaaa caatttttaa aatccatcaa acgcagcggc
     2941 ttcggcccca atgcctttga cgaatggcgt tacctcgacc acggcgaacc aggcatggac
     3001 aacagcaaac gcccgttgaa ccctgatttc tcgctgaacc agccgcgtta ccaaggcgcg
     3061 caaatcctgt tgacgcgtaa aaacttcggt tgcggctctt cacgcgaaca cgccccttgg
     3121 gcattggacg actacggctt ccgcgccgtc atcgccccca gcttcgccga catcttcttt
     3181 aacaactgct acaaaaacgg ccttttgcct atcgtgttga ccgaagaaca ggttgaccgg
     3241 cttttcaaag aagttgaagc caacgaaggc taccggctct ccatcgacct tgccgggcaa
     3301 accctgacca cccccggcgg cgaaacatta gcattcgaca ttaccgaaca ccgcaaacac
     3361 tgcctcttaa acggattgga cgaaatcgga ctgaccctgc aacacgccga tgaaattcac
     3421 gcctttgaag aaaaacgccg ccaaagccag ccttggctgt ttaacggtta aacctatgcc
     3481 gtctgaagac cgttcagacg gcattcctga taacatataa ttgctgcaac taatgtaaac
     3541 agaggggcta taaattgctt tcatctttca agcgggctta gcaacgctct aaattaatgc
     3601 aatacagctc tatttcaaat aaatttttag atttttaata tgcaacagat taaatttatt
     3661 gacttatttt ccgggatgag cggtatcagg aaaggatttg aacaagcctg tcggaaacag
     3721 tcggttgctt gcgaatgtgt ttttacctca gaaatcaaac cggcagcttt ggaagtattg
     3781 aagcaaaact accctgatga agtgccgtat ggagatataa cgaaaattga aacgggagat
     3841 attcccgatt ttgacatctt gttggcaggc ttcccttgcc aggctttttc tttcgccgga
     3901 aaaagattgg gctttgaaga tacgcgggga acgcttttct ttgatgtggc aaggatttta
     3961 aaggcaaaaa aaccaaaagg ttttattttg gaaaatgtgg aaggattggt gacgcacgat
     4021 agaaaagatc cgacacaaaa aataggacgc accctgaccg ttattttgga aaccttggaa
     4081 acattgggct actatgtttc ttggaaagtt ttaaatgcaa aagatttcgg cattccccaa
     4141 aacaggaagc gcatttatct gacaggcagt ttgaaatcca aacccgactt atcttttgaa
     4201 acaaccctaa gtccgaaatt aaaaaatatt ttggaatcgg gactgcctac cgaaagcagt
     4261 cctttcatca aaaaattgct aaagaaattt cccccgtccg aactgtacgg aaaatcagta
     4321 aaggacaaac ggggagggaa aaacaatatc cacagttggg atattgaatt aaaaggcaca
     4381 gtaaccgaag aagagaagca attattaaat atgcttctaa aagaacggag gaagaaaaaa
     4441 tgggcttcag aaatcggcat cgattggatg gatgggatgc ctttgacaaa agcgcaaatt
     4501 tcaactttct ataaacaccc caatcttcaa aatattttgg acagcctgac agataaaggc
     4561 tatttggttt tggagcatcc gaaacaaaaa attggcgggc gaagaatcaa agatgaatcc
     4621 ctgcccaaag gctacaatat tgtttcagga aaaaaatcat ttgaaatcaa taaaatatta
     4681 gacccaaatg atgttgcgcc aaccttagtt gcaatggata tggagcacct tttcgtcgtt
     4741 gacaacggcg gtttgcgcac acttaccgga aaagaaggtt tacgcttatt cggctatccg
     4801 gacgattatt cgtttgatat tcctaaaaaa gacaaatacg atttattggg caataccgtt
     4861 gccgttcctg tgattaaggc ggtatctgaa aggcttctgc atactttata aaccgatagg
     4921 gataaaatgg taaaacttac tgcacaacaa atatttgata agttgctgaa tgaggaaaaa
     4981 atcttatcgg tcaatggtca aattagattt ttcttgggcg atgtggatat tatcgtcaaa
     5041 caaaaagatg tcgtcggcaa catcattcag gaatggctcg gcggatggtt gagaaaaagg
     5101 gaaattgaat ttgatgtttc aaccaatacc caaatgcccc ccgatttctt tttaaataaa
     5161 aaagaccgca gtagagaatt gttggaagta aaggcgttca accgaaatgc cagtccgggt
     5221 tttgatattg cagattttaa aatgtattct gatgaaatca ttcataaacc ctatatgctg
     5281 gatgtagatt atttaatatt cggttacgat atggacgaca acggcaatgt aaccatcaag
     5341 gatttatggc ttaaaaaagt atggcaaatt acgagaagca tggatgggtg ggcaattaat
     5401 cttcaagtca aaaaaggcgt ggtgcataaa atccgcccgg gtgtttggta cagcataaat
     5461 aaaaagaata tgcctatgtt tgaatgcttg gaagattttg tttccgcaat tgaagaaacc
     5521 gtttatcaaa acccggcaac acggcataac gcatccttat ggaaaaagaa atttgaggaa
     5581 gcctataaaa aacactacaa ccgatcgatt tccatacctc gttggcacga aattgcacac
     5641 aaatacaaaa agaaataacc cttcaaccat caacccataa ggaaacttga aatgaccaaa
     5701 catatcgcca tcctccgagg tgacggcatc ggtcccgaaa tcgtcgccga aaccgtccgc
     5761 gtactcgaca aactcatcgc ccaaggtttg gatgccggct acgaatacgc cccattgggc
     5821 ggcgaagcct atgacgaata cggtcatcct tatcccgaat ttacgcaaaa cctctgccgc
     5881 aaagccgatg ccgtcctgct tggtgcagtc ggttctcctc aatacgacaa cctcgaccgt
     5941 ccgttgcgcc ctgagcgcgg cctgttggca atccgtaagg atttgaacct gtttgccaat
     6001 ttgcgtcctg ctgttttata tcccgaatta gccaacgcct ccacgctgaa gcctgaaatc
     6061 gttgccggtt tggatatttt gattgtgcgc gaactcaccg gcgacatcta tttcggcgaa
     6121 ccgcgcggca tccgcgttct ggaaaacggc gaacgcgaag gctacaacac catgaaatac
     6181 agcgaaagcg aaatccgccg tattgcccat gttgccttcc aatccgccca aaaacgcagc
     6241 aaaaaagtct gttccgtagg caaagccaac gttttggaaa ccaccgaatt gtggcgcgaa
     6301 atctttgaag aaatcggcaa agagtaccct gatgtcgagc tttcccatat gtacgtcgac
     6361 aacgccgcca tgcagctcgt gcgtgcgccc aaacaattcg acgtcattgc caccggcaac
     6421 atcttcggcg acattctctc cgacgaagcc tccatgctga ccggctccat cggtatgctg
     6481 ccttccgcct cgctggacga aaacggcaaa ggcctgtacg aaccgtctca cggctccgcc
     6541 cccgacatcg ccggacaaaa caaagccaac ccgctggcca ccatcctctc gctcgcaatg
     6601 ttgctgcgtt acagcctgaa cgacgaagcg cgcgcgcaac aagttgaaaa cgccgtccaa
     6661 aaagtgctgc aacaaggctt gcgtaccagc gacatttacg aagaaggcac aaaactcgtt
     6721 tcctgctccg aaatgggcga tgcggtactc gccgccttgt aaaaaaacaa agcgcggatc
     6781 acaccgcgct ttttcatccg cccttttccg cccgccccgt tcgtcaaccg cctgcaaacc
     6841 caccgtttac cggcaggaaa tgccataaat ccctgcctat gttaggataa acgcctttac
     6901 cggcccacac aaaggattat catcatgaaa aaaatcatct tcgccgcact cgcagccgcc
     6961 gccatcagta ctgcctccgc cgccacctac aaagtggacg aatatcacgc caacgcccgt
     7021 ttcgccatcg accatttcaa caccagcacc aacgtcggcg gtttttacgg tctgaccggt
     7081 tccgtcgagt tcgaccaagc aaaacgcgac ggtaaaatcg acatcaccat ccccattgcc
     7141 aacctgcaaa gcggttcgca acactttacc gaccacctga aatcagccga catcttcgat
     7201 gccgcccaat atccggacat ccgctttgtt tccaccaaat tcaacttcaa cggcaaaaaa
     7261 ctggtttccg ttgacggcaa cctgaccatg cacggcaaaa ccgcccctgt caaactcaaa
     7321 gccgaaaaat tcaactgcta ccaaagcccg atggagaaaa ccgaagtttg tggcggcgac
     7381 ttcagcacca ccatcgaccg caccaaatgg ggcatggact acctcgttaa cgttggtatg
     7441 accaaaagcg tccgcatcga catccaaatc gaggcagcca aacaataaat cttgatgcga
     7501 atcagcacaa atgccgtctg aacgtccttc aaacggcatt tgtgccgtct tcatcaaacc
     7561 gccccgccaa cggtaagccg atatggcgga gcacaatccg ccgaaagata tttcagcggc
     7621 aggcagggct tgccggaaag tccggaaa
//
LOCUS       DAWHSK010000080         7624 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.080, whole genome shotgun sequence.
ACCESSION   DAWHSK010000080 DAWHSK010000000
VERSION     DAWHSK010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7624
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.080"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(104..784)
                     /gene="tsaA"
                     /locus_tag="WIC03_001069"
     CDS             complement(104..784)
                     /gene="tsaA"
                     /locus_tag="WIC03_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HEZ3883579.1"
                     /translation="MTYTIVPIATARSPYKQKFGIARQPGLVSAAEVCIELNPEFTAD
                     SVRGLEHFDYVWISFIFHGVLDEGWAQMVRPPRLGGKQKMGVFATRSPHRPNHLGLSL
                     LKLERIETGKPVRLYCSGADLLDGTPIVDIKPYIPFVESKPDAASGFVSGKPVELEVV
                     WQENIGAENLSANTKNLISQSIAQDPRPAYQNIPERIYVMNIADYEVRFQIEENRATV
                     IDLSPTPL"
     gene            899..1534
                     /locus_tag="WIC03_001070"
     CDS             899..1534
                     /locus_tag="WIC03_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamidase"
                     /protein_id="HEZ3883580.1"
                     /translation="MIVSIDVDAQKTFTPLCPKELPVNEGHLIVEELNAQAALADLRV
                     MTKDAHHAAAKWLVDNPVDMLKPTGLPDADLTWVAHAMVGTRGYELLDGLPCAKEYDY
                     CVWKGIDPELHPYGACFHDIEEKLSTGLIEWLRCQNTDTVIIGGLATDYCVKTTVLQL
                     LKGGRWQVVVNEAACRGIAPETIETAWQEMHSAGAIILKNADEINKYINNQ"
     gene            1571..2539
                     /gene="waaC"
                     /locus_tag="WIC03_001071"
     CDS             1571..2539
                     /gene="waaC"
                     /locus_tag="WIC03_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HEZ3883581.1"
                     /translation="MKILLVRLSSMGDLIHTLPAIEDLARQCPDVELHWLCEAGFADI
                     ARLHPFVKKIHVMKWRQWRKHLFRAETWREMGHLKQTLRQEVFDFVLDSQGLIKSACF
                     AKMAKSPIYGLDKNSAREGWAALAYVKTYAVPKGKNAVWRNRELFAQVFGYVMPETQV
                     FGLTVPEAGRLKNLAQPYYAALHATSRDSKLWPVENWRSLLQKLNEEQQCNIYLPWGN
                     EDEKTRAKQIADGLPFAIVCDKINLLQAAYLLKYAVGIVGVDTGLLHLANALEKPVVG
                     IYTDTDPIKTGVQVSPIAKNLGNIGQIPTADLVYQALMDCVAADKG"
     gene            2766..3515
                     /locus_tag="WIC03_001072"
     CDS             2766..3515
                     /locus_tag="WIC03_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEZ3883582.1"
                     /translation="MKALVAVKRVVDYNVKVRVKADGSDVDIGNVKMSMNPFDEIAVE
                     EAVRLKEAGKVSEIVAVSLGEKKCEETLRTALAMGADRAIHVETDAKLEPLAVAKLLK
                     AVADKENPQIFFLGKQAIDDDANQVAQMLAALLNAAQGTFASKVQIEGDEVQIVREID
                     GGEETIALKLPAVISADLRLNEPRFVKLPNIMAAKKKPLEKLAPADLATDILPRLKTV
                     KFAEPKARQAGVKVASVAELVEKLKNEAKVI"
     gene            3526..4461
                     /locus_tag="WIC03_001073"
     CDS             3526..4461
                     /locus_tag="WIC03_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEZ3883583.1"
                     /translation="MSVLIIVEHDNKQLNPTTLHAVTAAAKLGKVDLLVAGSGASAVV
                     ESAKQVAGVEKVLVADAAHYAEGLAEELAPLVVKLAADYRYVAATATTFGKNLLPRVA
                     ALLDVPQISDLTEIVDNTTFVRPIYAGNAFETVQANAEKLVLTFRATAFDAAAVQGGN
                     AEVINIETTPAQNLSRFVNRQLSHSDRPELTQAKVIVSGGRALGSAEKFNEVLTPLAD
                     VLGAAIGASRAAVDAEYAPNDAQVGQTGKVVAPQLYFAIGISGAIQHVAGMQDSKVIV
                     AINKDADAPIFNVADYGLVGDLFEIVPQLTEALKN"
     gene            4524..5135
                     /locus_tag="WIC03_001074"
     CDS             4524..5135
                     /locus_tag="WIC03_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEZ3883584.1"
                     /translation="MITTYKTITSPTQAGFKDKGSRFIAFAYPIRTLADVKKYLDPLK
                     EEHHKARHWCYAYRLGVDGMQFRANDDGEPSGSAGRPILGQIDSVGITDVLVVVVRYF
                     GGTLLGVSGLIHAYKEATAQALAIAEVIEKNIEKTVWLKCEYPFLNEAIRIAKQYQAD
                     ILEQDLQLDCRLTVSLSLANYEVCVAAWKNTRQIEVNTEKPFE"
     gene            complement(5187..5504)
                     /locus_tag="WIC03_001075"
     CDS             complement(5187..5504)
                     /locus_tag="WIC03_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HEZ3883585.1"
                     /translation="MQKRIDEIQSKYREWCHLLPQLEEDIRRWKHAVALIRDMDNFYT
                     HEYQACHRAIEDGAEVDLRTEGEYSIMSEDALWNALGEFHQLAWLYLRSSVDALDKYT
                     QED"
     gene            5662..6933
                     /gene="purD"
                     /locus_tag="WIC03_001076"
     CDS             5662..6933
                     /gene="purD"
                     /locus_tag="WIC03_001076"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEZ3883586.1"
                     /translation="MKLLVIGNGGREHALAWKLAQSPKVETVFVAPGNAGTAIEPKLQ
                     NIDLTAHQDLIEFCRKENIVFTVVGPEAPLAAGVVDDFRAAGLKIFGPTQYAAQLESS
                     KDFAKAFMAKYNIPTAQYQTFENADAAHDYVNQKGAPIVIKADGLAAGKGVIVAMTLD
                     EAHAAIDDMLLDNKMGNAGARVVIEDFLQGEEASFIVMVDGNNVLPMATSQDHKRLLD
                     GDKGLNTGGMGAYSPAPVVTPVVYERAMNEIILPTVAGMKAEGHEFTGFLYAGLMIDQ
                     SGAPYTIEFNCRFGDPETQPIMSRLNSDLADLVEAAIDGKLDSVAAEWNPQTAVGVVL
                     AAQNYPETPKKGDVISGLDAANQVGKVFHAGTTANEKGDVLTNGGRVLCVVGLGDNVA
                     QAKAKAYGALEKISFDGMQYRKDIADKAINR"
     gene            6967..7569
                     /locus_tag="WIC03_001077"
     CDS             6967..7569
                     /locus_tag="WIC03_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEZ3883587.1"
                     /translation="MTDMLQTAFYIMLFPRIIAASAQRKLSVYLKKGGLVAYPTESCY
                     GLGCLPTLAKALGKLAHLKKRPQHKGMIVIGNQFEQLQPLLQMPSENLQDMLRKEWPA
                     PKTFLLSAKSCVLPELRGKQRSKLAVRVPAHVGARRLCQALQTPLVSTSCNRAGKRAC
                     RTEREVRRQFGRDVWIVGGRIGRQKSPSQIIDGETGKRLR"
BASE COUNT         2008 a         1516 c         2055 g         2045 t
ORIGIN      
        1 tgatttaaat ttaatccgct ataacttgat attgcggctt atggcgaaca aacaacagcc
       61 gtttgttcaa ctgctatgcg gcaaaaccgg atttttgccc gatttaaagc ggggttgggg
      121 aaagatcaat aacggttgca cggttttcct cgatttgaaa tctgacttcg taatctgcaa
      181 tattcatcac ataaatccgt tcgggaatat tctgataggc ggggcgcgga tcttgggcaa
      241 tgctttggct gataaggttt ttggtgtttg cagataaatt ttccgcgccg atgttttcct
      301 gccaaacgac ttccaactct acgggtttgc cgctgacgaa accagatgcg gcatcgggtt
      361 tggattcgac aaaggggata taaggcttga tgtccacaat cggcgtgccg tccagcaggt
      421 ctgcgccgct gcaatagagg cggacgggtt tgccggtttc gatgcgttcg agtttcagga
      481 gcgagagtcc gagatggttg gggcggtggg ggctgcgcgt ggcgaatacg cccatttttt
      541 gtttgccgcc gagccgtggc gggcgcacca tttgtgccca gccttcatcc aatacgccgt
      601 gaaaaataaa acttatccac acatagtcga agtgttccag tccgcgtacg ctgtctgcgg
      661 taaattcggg attcagctcg atgcatactt ctgcggcaga gaccaaaccg ggctggcggg
      721 cgatgccgaa tttctgtttg tagggcgatc gggcggtggc gatagggacg atggtgtaag
      781 tcatgaggat tgtggataag tttttcggaa aaaccttatc ttatcacagc ctgttttacc
      841 gcttttgggc agtaatttgt tataatacag ggcattttaa ttgcgttgaa atgatgagat
      901 gattgtttcc attgatgttg atgcacaaaa aacttttacg cctttgtgtc ctaaagagct
      961 gcctgtaaac gaggggcatt tgattgtcga agaattgaat gcccaagccg ctttggcgga
     1021 tttgcgcgtg atgacgaaag atgcgcatca tgcggcagca aaatggcttg tggataaccc
     1081 tgttgatatg ttgaagccga caggtttgcc cgatgcggat ttgacttggg tggctcatgc
     1141 gatggttggt acgcgcggct atgaattgtt ggacgggctg ccttgtgcca aagaatacga
     1201 ttattgcgtt tggaaaggta ttgatcctga attgcatcct tacggcgcgt gttttcacga
     1261 tattgaggaa aaactaagca cagggctgat tgaatggctg cgttgtcaaa atacggatac
     1321 ggttattatc ggtgggttag ctacggatta ttgtgttaaa acaacggttt tgcagttact
     1381 caaaggcggt cgttggcagg ttgtcgtcaa tgaagcggct tgtcggggta ttgcgccgga
     1441 aaccatcgaa acagcatggc aggaaatgca ttctgccggg gcaatcatct taaaaaacgc
     1501 tgatgaaata aataaatata ttaataatca ataagttaaa attgtttcac gtgaaacccc
     1561 cttcctaaat atgaaaattt tgcttgtccg cttgtctagt atgggcgatt taattcacac
     1621 tttgcccgca atcgaagatt tggcgcgaca atgtcctgat gtagaactgc attggctatg
     1681 tgaggctgga tttgcagata ttgcgcgcct gcatccgttt gtaaaaaaaa tccatgtgat
     1741 gaaatggcgg caatggcgca aacatctctt tcgggctgaa acttggcggg aaatgggtca
     1801 tctgaaacag actttgcggc aggaagtatt tgatttcgta ttggacagtc aaggtctgat
     1861 taaaagcgcg tgtttcgcta aaatggcgaa atcccctatt tatggtttgg ataaaaacag
     1921 tgcgcgcgag ggatgggctg ctttggcgta tgtaaaaaca tacgctgtac cgaagggaaa
     1981 aaatgccgtt tggcgcaacc gtgaactgtt tgcccaagta tttgggtatg taatgccgga
     2041 aacgcaggta tttggcttga ctgttcctga agcaggccgt ctgaaaaatt tagcgcagcc
     2101 gtattatgcg gctttgcatg cgaccagtcg ggacagtaag ttatggcctg tggaaaactg
     2161 gcggtcgttg ctgcaaaagt tgaatgaaga acagcaatgc aatatttacc tgccttgggg
     2221 aaatgaagat gaaaaaacgc gtgccaaaca aattgcagac ggactgccgt ttgctattgt
     2281 gtgcgacaaa ataaatttat tgcaggcagc gtatctgctg aaatacgcgg tcggaattgt
     2341 cggcgtggat accggtttgc tgcatttggc aaatgccttg gaaaaacctg tggtcggcat
     2401 ttataccgat accgatccga ttaaaacagg cgttcaggtt tcgccaattg caaaaaattt
     2461 gggcaatatc gggcagattc cgaccgcaga tttggtttat caagcgctga tggattgtgt
     2521 tgcagcagac aaaggctaaa gggttgtctg aaactgatat ggcggcatga tattttccaa
     2581 tttattaaat acaggaatgt gtatccgcta gtctgagtaa atgctttaaa aatggtataa
     2641 tcggcaagca ttatccgaaa gatttttgaa tgaattcgtt tttaaggaaa acggatttgg
     2701 ttttataggg aagaaggttt ggtttcgctg gatggtgaaa gcagcgttct ttttaggagg
     2761 gttcaatgaa agcactggtc gcagtaaagc gcgtagtgga ctacaacgtc aaagttcgtg
     2821 taaaagccga tggttcggat gtggatatcg gcaatgtcaa aatgtcgatg aatccgtttg
     2881 acgaaatcgc tgtggaagaa gccgtccgtt tgaaagaagc cggaaaagta agcgagatcg
     2941 tagcggtttc tttgggcgaa aagaaatgcg aagaaacgtt gcgtaccgct ttggcgatgg
     3001 gtgccgaccg tgcaattcat gttgaaaccg atgcgaaatt ggagccgctg gcagttgcca
     3061 agttgctgaa agccgttgcg gacaaagaaa atccgcagat tttctttttg ggcaaacagg
     3121 cgattgatga tgatgccaac caagtggcgc aaatgctggc tgctttgctg aatgcggcgc
     3181 aaggtacgtt cgcgtccaaa gtacaaattg aaggcgacga agtgcagatt gtgcgggaaa
     3241 tcgatggcgg cgaagaaacc atcgcattga aactgcctgc tgttatcagt gctgatttgc
     3301 gtttgaacga gccgcgcttt gtcaaactcc ccaatattat ggcggcaaag aaaaaacctc
     3361 tggaaaaact ggcgcctgcc gatttggcta ccgatatttt acctcgtctg aaaacggtga
     3421 aatttgccga acctaaggcg cgtcaggcag gcgtaaaagt agcaagcgtt gccgaattgg
     3481 ttgaaaaatt gaaaaacgaa gccaaagtga tttgaggagg ctgaaatgag cgtattgatt
     3541 attgtcgaac acgacaacaa acagttgaat cctaccactt tgcatgctgt taccgctgcc
     3601 gccaagctgg gtaaagtcga tttattggtt gccggaagcg gtgcatctgc cgtagtagag
     3661 tccgcaaaac aagtggcggg tgtggaaaaa gttttggttg cagatgctgc ccattatgcc
     3721 gaaggtttgg ctgaagagtt ggctccgctg gttgttaaat tggcagcgga ttaccgctat
     3781 gttgcagcaa cggcaaccac attcggtaaa aaccttttgc cccgcgtagc cgccttatta
     3841 gacgtaccgc aaatttctga tttgaccgag attgtagata acacgacttt tgtacgtccg
     3901 atttatgcag gtaatgcatt tgaaaccgtg caagccaatg cggaaaaatt ggtgctgacc
     3961 ttccgtgcga cggcttttga cgcagcggcg gtgcaaggtg gtaatgctga ggtaattaat
     4021 attgaaacaa cccctgccca aaacctgagt cgttttgtta accgtcagct ttcccattcc
     4081 gatcgtcccg aactgactca ggcaaaagtg attgtttctg gtggtcgtgc gttgggtagt
     4141 gcggaaaaat ttaatgaagt gctgacaccg ttggcggatg ttttaggtgc ggcaatcggt
     4201 gcatcccgtg cggcagttga tgccgaatat gcgccaaacg atgctcaagt cggacaaacc
     4261 ggtaaagtag ttgcgccgca actctatttt gcaatcggta tttcaggtgc gattcaacac
     4321 gttgccggta tgcaagacag taaggtgatt gttgcaatca ataaagatgc tgatgcgccg
     4381 attttcaatg tagccgatta cggattggtt ggcgatttat ttgaaatcgt tccacaatta
     4441 acagaagcat tgaaaaatta atgtttcacg tgaaactttc agacgacttt gttattaatg
     4501 aggttgtctg aaaacttttc aacatgatta cgacttataa aaccattact tccccgacgc
     4561 aggctgggtt taaagataaa ggcagccgtt ttatcgcatt tgcctatccg attcggacat
     4621 tggctgatgt gaaaaaatac ctcgatccgt taaaggaaga gcaccataaa gcacgacact
     4681 ggtgttatgc ctatcgtttg ggcgtggatg gtatgcaatt tcgtgccaac gatgatggag
     4741 agccatcagg aagtgctgga cgaccaattt tgggacagat tgattcggtg ggcatcaccg
     4801 atgttttggt cgtggtcgtc cgctatttcg gtggtacttt attgggcgtt tcaggtctga
     4861 tacatgcgta caaagaggcg acggctcagg cgttggcgat tgcggaagtg attgaaaaga
     4921 atattgaaaa aaccgtttgg ctgaaatgtg aatatccatt tttgaatgaa gcgatacgca
     4981 tcgccaaaca atatcaggcg gatatattgg agcaggattt gcagttggat tgccgattga
     5041 cggtaagttt gtcgttggca aattatgaag tctgtgttgc ggcttggaag aatacacggc
     5101 agattgaagt aaatacggaa aagccttttg aatgaaaggt cgtctgaaat gctttgtgat
     5161 ggtatttcag acgacctctt cgctgactaa tcttcttgtg tatatttgtc taaggcatcg
     5221 acgctggagc gcaaatataa ccaagccagt tgatggaatt cgcccagcgc gttccatagc
     5281 gcatcttcac tcataatgct gtattcgcct tctgtgcgca aatccacctc cgctccatct
     5341 tcaatagccc gatgacaagc ctgatattcg tgggtataaa aattgtccat atcgcgaatc
     5401 aaagcgacgg catgtttcca acggcggatg tcttcttcca attgcggtag taaatgacac
     5461 cattcgcggt atttgctttg gatttcatca atacgttttt gcatggttgc ttgtcctttt
     5521 ttaatttgtg aataagttga ataggttgca gagtggaatg aagcggtctt ttgtttgatt
     5581 ctactaaaat ttcttcagat gggataaaag tccctttgta tcgagttttc gttaaaagta
     5641 catatttgtc aaggagatgg catgaaactg ctggttatcg gtaatggcgg tcgcgaacac
     5701 gcgctggctt ggaaattggc gcagtcgcct aaagtggaaa cagtatttgt tgcgcctggt
     5761 aatgccggta cagcgattga acccaagttg caaaacatcg acttgactgc gcatcaggat
     5821 ttgattgaat tctgtcgtaa agaaaatatt gtttttactg tcgtcggccc tgaagcgcct
     5881 ttggcggcag gtgttgtgga tgatttccgt gccgcaggac taaaaatatt cggtccgaca
     5941 caatatgcgg cgcagttgga aagttccaaa gatttcgcca aagcatttat ggcgaaatac
     6001 aatattccaa ccgcgcaata tcaaaccttt gaaaacgccg atgccgcaca tgattacgtc
     6061 aatcagaaag gtgcgcctat cgttatcaaa gccgatggtt tggcggcagg taaaggcgtg
     6121 attgtggcga tgactttgga tgaagcgcat gctgcgattg atgatatgct gctggacaac
     6181 aaaatgggca atgccggcgc gcgtgttgtg attgaagatt tcctgcaagg cgaagaagcg
     6241 agttttatcg tcatggttga tggcaataat gtactgccta tggcaaccag ccaagaccac
     6301 aagcgtcttt tagacggcga caaaggtctg aatacgggcg gtatgggtgc gtacagccct
     6361 gcgcctgtgg taacgcctgt tgtgtacgaa cgcgcgatga atgaaattat tttgccgacc
     6421 gtagcaggga tgaaagcgga ggggcatgag ttcaccggtt tcctgtacgc aggtttgatg
     6481 attgatcaaa gcggtgcacc ctatacgatt gagtttaact gccgtttcgg cgatcctgaa
     6541 acccagccga ttatgagtcg tctgaacagc gacttggcgg atttggttga agcggcaata
     6601 gacggtaaac ttgatagtgt tgctgccgaa tggaatccac aaactgccgt aggtgtagtg
     6661 ttggcggcgc aaaactatcc tgaaacgcct aaaaaaggcg atgttatttc cggtctggat
     6721 gctgccaacc aggtaggcaa agttttccat gcaggtacaa cggcaaacga gaaaggtgac
     6781 gtattgacca acggcggacg cgtattgtgt gttgtgggat tgggcgacaa tgtggcgcaa
     6841 gcgaaagcta aggcatatgg cgcgttggaa aaaatcagct tcgacgggat gcaataccgt
     6901 aaagacattg ccgataaagc gattaaccgt taattagaaa gaggatgatg ccgtctgaag
     6961 ccaagtatga ctgatatgct tcagacggca ttttatatta tgttatttcc gagaattatc
     7021 gctgcatcag cgcaacgtaa gttaagcgtt tatttaaaaa aaggcggttt ggtcgcttat
     7081 ccgaccgaat cttgttatgg cttggggtgt cttccgacat tagccaaagc actcggtaag
     7141 ttggcgcatt tgaaaaaaag accgcaacac aagggtatga ttgtcatcgg gaatcaattt
     7201 gaacaattgc agcctttact tcaaatgccg tctgaaaatc ttcaggatat gcttagaaaa
     7261 gaatggcccg cccctaaaac gttcctatta tccgccaaat cctgtgtctt acctgagttg
     7321 cgcgggaaac agcgcagcaa gttggcggtc agggttcccg cccatgtagg tgcgcgccgc
     7381 ttgtgtcagg ctttgcaaac gcctttggtt tcgacttcgt gcaaccgcgc aggcaaacgc
     7441 gcgtgtcgga cggagaggga agtcagaagg caattcggac gcgatgtgtg gattgttggt
     7501 gggagaatcg gcaggcagaa atcgccgagt cagattattg acggggaaac aggtaaacgt
     7561 ttgcgttgag acctttgcaa aattcctttc cctcccgaca gccgaaactc aaacacaggt
     7621 tttc
//
LOCUS       DAWHSK010000081         7553 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.081, whole genome shotgun sequence.
ACCESSION   DAWHSK010000081 DAWHSK010000000
VERSION     DAWHSK010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7553
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.081"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            24..419
                     /locus_tag="WIC03_001078"
     CDS             24..419
                     /locus_tag="WIC03_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEZ3883588.1"
                     /translation="MFGLHKSFGFLTLTVITLRIVWAVANRAKRPQSDSKAAAAGHGI
                     LYLLMLAVPVIGMIRQYGSGRGPLKVFGVEVMQGSPEKIEWMANLGNTFHGNLGWLLF
                     AAVVGHVAMVVVHRVQGKDVLYRMTGRVR"
     gene            complement(454..1329)
                     /gene="rsmI"
                     /locus_tag="WIC03_001079"
     CDS             complement(454..1329)
                     /gene="rsmI"
                     /locus_tag="WIC03_001079"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEZ3883589.1"
                     /translation="MFQKHLQKASDSVVGGTLYVVATPIGNLADITLRALAVLQKADI
                     ICAEDTRVTAQLLSAYGIQGKLVSVREHNERQMADKIVGYLSDGMVVAQVSDAGTPAV
                     CDPGAKLAGRVREAGFKVVPVVGASAVMAALSVAGVEGSDFYFNGFVPPKSGERRKLF
                     AKWVRAAFPIVMFETPHRIGATLADMAELFSERRLMLAREITKTFETFLSGTVGEIQT
                     ALSADGNQSRGEMVLVLYPAQDEKHEGLSESAQNIMKILTAELPTKQAAELAAKITGE
                     GKKALYNLALSWKNK"
     gene            1376..1723
                     /locus_tag="WIC03_001080"
     CDS             1376..1723
                     /locus_tag="WIC03_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEZ3883590.1"
                     /translation="MRLNHKQGEAGEDAALAFLQSQGCTLLARNWHCAYGEIDLIVKN
                     GGMILFVEVKYRKNRQFGGAAYSISPSKLLKLQRSVEYYLQQNRLTNVPCRLDAVLIE
                     GNRPPEWIQNITG"
     gene            1728..2321
                     /locus_tag="WIC03_001081"
     CDS             1728..2321
                     /locus_tag="WIC03_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HEZ3883591.1"
                     /translation="MTTLQERVAAHFAESIRAKQEAEKVLVEPTVQAAELMLQCLMND
                     GKILACGNGGSAADAQHFAAEMTGRFEKERMELAAVALTTDTSALTAIGNDYGFDHVF
                     SKQVRALGRAGDVLVGISTSGNSANVIEAVKAAHERDMHVIALTGRDGGKIAAILKDT
                     DVLLNVPHPRTARIQENHILLIHAMCDCIDSVLLEGM"
     gene            2385..2993
                     /locus_tag="WIC03_001082"
     CDS             2385..2993
                     /locus_tag="WIC03_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEZ3883592.1"
                     /translation="MKLKLHTVRILTAAIFSLALSGCVSAVIGGAAVGAKSAVDRRTT
                     GAQTDDNVMALRIETTARSYLRQNNQTKGYTPQISVVGYNRHLLLLGQVATEGEKQFV
                     GQIARSEQAAEGVYNYITVASLPRTAGDIAGDTWNTSKVRATLLGISPATQARVKIVT
                     YGNVTYVMGILTPEEQAQITQKVSTTVGVQKVITLYQNYVQR"
     gene            3001..3651
                     /locus_tag="WIC03_001083"
     CDS             3001..3651
                     /locus_tag="WIC03_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883593.1"
                     /translation="MPSEPPSDGIARHPKSTIKMAKKPNKPFRLTPKLLIRVVLLICI
                     TAIGALAIGIVSTFNPNGGKTLQTEPQHTDSPRETEFWLPNGVVGQDAAQPEHHHAAS
                     SEPAQPDGTDESGSGLPSPAAPKKNRVKPQPADTAQTDRQPDDAGTQAENTLKETPVL
                     PTNVPRPEPRKETPEKQAQPKETPKEKETPKENHTKPDTPKNTPPKPHKEILDNLF"
     gene            3691..4470
                     /gene="map"
                     /locus_tag="WIC03_001084"
     CDS             3691..4470
                     /gene="map"
                     /locus_tag="WIC03_001084"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEZ3883594.1"
                     /translation="MNGIIIKTPEEIEKMRELGKLVAEALDYIGQFVKPGVTTDEIDK
                     LVYDYHVNVQGGYPAPLHYGNPPYPKSCCTSVNHVICHGIPDDKPLKEGDIINIDLTI
                     KKDGFHGDSSRMFTVGKVSPIAQRLIDVTHASMMAGIEAVKPGATLGDVGYACQQVAE
                     NAGYSVVQEFCGHGIGRGFHEAPQVLHYGKKGQGPVLKPGMIFTVEPMINQGKRHLRI
                     LNDGWTVVTKDRSLSAQWEHEVLVTETGYEILTVSPASGKP"
     gene            4625..4924
                     /locus_tag="WIC03_001085"
     CDS             4625..4924
                     /locus_tag="WIC03_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEZ3883595.1"
                     /translation="MKIFENIEDVKAIRKKTGLNQIDFWGKVGVTQSGGSRYETGRKM
                     PKPVRELLRLVHIECIDLAKVNKKDMEIAALLKKHHPDLYAELSKQTKSERKKQS"
     gene            5162..5536
                     /locus_tag="WIC03_001086"
     CDS             5162..5536
                     /locus_tag="WIC03_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HEZ3883596.1"
                     /translation="MKLLTTAILSSAIALSSMAAAAGTDNPTVAKKTVSYVCQQGKKV
                     KVTYGFNKQGLTTYASAVINGKRVQMPVNLDKSDNVETFYGKEGGYVLGTGVMDGKSY
                     RKQPIMITAPDNQIVFKDCSPR"
     gene            complement(5724..7190)
                     /locus_tag="WIC03_001087"
     CDS             complement(5724..7190)
                     /locus_tag="WIC03_001087"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HEZ3883597.1"
                     /translation="MAEATDVVLVGGGIMSATLGVLLKELEPSWEITLIERLEDVALE
                     SSNAWNNAGTGHSALCELNYAPLGANGIIDPARALNIAEQFHVSRQFWATLVAEGKLE
                     DNSFINAVPHMSLVMNEDHCSYLQKRYDAFKTQKLFENMEFSTDRNKISDWAPLMMRG
                     RDENQPVAANYSAEGTDVDFGRLTRQMVKYLQGKGVKTEFNRHVEDIKRESDGAWVLK
                     TADTRNPDGQLTLRTRFLFLGAGGGALTLLQKSGIPEGKGYGGFPVSGLFFRNSNPET
                     AEQHNAKVYGQASVGAPPMSVPHLDTRNVDGKRHLMFGPYAGFRSNFLKQGSLMDLPL
                     SIHMDNLYPMLCAGWANMPLTKYLLGELRKTKEERFASLLEYYPEANPDDWELITAGQ
                     RVQIIKKDSEKGGVLQFGTEIVAHADGSLAALLGASPGASTAVPLMIRLMHQCFPERA
                     PSWEDRLKELVPGYGIKLNENPERADEIIAYTAKVLDI"
BASE COUNT         1949 a         2274 c         1847 g         1483 t
ORIGIN      
        1 aagaggcgga atgggcgggc agcctgttcg gcctgcacaa atctttcggc ttcctcacgc
       61 tgacggtgat tacattgcgc atcgtgtggg cagttgccaa ccgcgccaag cgtccgcaaa
      121 gcgacagcaa ggctgcggcg gcaggacacg gcattctgta tctgctcatg cttgccgttc
      181 ccgttatcgg catgatccgc caatacggca gcggccgcgg cccgttgaaa gtgttcggcg
      241 ttgaggtgat gcagggttcg ccggaaaaaa tcgagtggat ggcaaacttg ggcaacacgt
      301 tccacggcaa tttgggctgg ctgctgtttg ccgccgtcgt cggacacgtc gccatggtcg
      361 tcgtccaccg tgttcaaggc aaagacgtgc tgtaccgcat gacggggcgt gtccgttgat
      421 tccgttcgca ctatggtgcc ggtccgtccg gcatcatttg tttttccaag acagtgccag
      481 attgtacaaa gctttttttc cctcgcccgt gattttggcg gcaagctccg ccgcctgttt
      541 ggtcggcagc tcggctgtga ggattttcat gatgttttgc gcggactcgg acaagccttc
      601 gtgtttttca tcctgcgccg gataaagcac caacaccatc tcaccgcgcg attggttgcc
      661 gtcggcagac aatgccgtct gaatttcccc aaccgtgccg cttaagaacg tttcaaacgt
      721 tttcgtaatt tcgcgcgcca gcattaatcg gcgttcggag aacagttccg ccatatcggc
      781 aagcgtcgca ccgatgcggt gcggcgtttc aaacatgacg ataggaaacg ccgcccgcac
      841 ccatttggca aacagtttcc tgcgttctcc cgatttcggc ggtacaaaac cgttgaaata
      901 aaaatcggat ccttccacac cggccacgct caaagccgcc atcaccgcgc ttgcgcccac
      961 gacgggaacg actttaaacc cggcctcacg cacgcggccg gcgagtttgg cacccgggtc
     1021 gcacacggcc ggcgtacccg catcggaaac ctgtgccaca accatgccgt ctgaaagata
     1081 gccgacaatc ttgtccgcca tctgccgttc gttgtgttcg cgcacactga caagtttgcc
     1141 ctgaatgccg tacgcgctca aaagctgcgc ggtaacgcgc gtgtcttcgg cacagatgat
     1201 gtccgccttt tgcaataccg ccaaagcgcg cagggtaatg tccgccaaat tgccgatggg
     1261 cgtggcaacc acgtataatg tccctccgac gacgctgtcg gaggctttct gcaagtgttt
     1321 ctgaaacata aaaaatgccg tctgaaaaac aaacattata aaggttaaac tgattatgcg
     1381 cctaaaccac aaacagggcg aggcaggcga agatgccgca cttgccttcc tccaatccca
     1441 aggctgcacg ctgcttgccc gcaactggca ctgcgcctac ggcgaaatcg acctgattgt
     1501 caaaaacggc ggcatgattc tgtttgttga agtaaaatac cgcaaaaatc ggcaattcgg
     1561 cggtgccgca tacagcattt cgccgtccaa attattgaaa ctgcaacgaa gtgtagagta
     1621 ttatctgcaa cagaacaggt tgacaaacgt accgtgccgc ctcgatgcgg tacttatcga
     1681 aggaaaccgc ccgcccgagt ggatacagaa tattacaggt tgacgatatg acgacattac
     1741 aagaacgcgt tgccgcccat tttgccgaaa gcatccgtgc caagcaagaa gccgaaaaag
     1801 tactggtcga gccgaccgta caggctgccg agctgatgct gcaatgcctg atgaatgacg
     1861 gcaaaatcct ggcgtgcggc aacggcggtt cggctgccga cgcacagcac ttcgccgccg
     1921 aaatgaccgg gcgttttgaa aaagaacgta tggaactcgc cgccgtcgcg ctgaccaccg
     1981 acacctccgc gctgacagcc atcggcaatg actacggctt cgaccacgtc ttcagcaaac
     2041 aagtacgcgc cctcggacgc gccggcgacg ttctcgtcgg catttccact tccggcaatt
     2101 ccgccaacgt catcgaagcc gtcaaagccg cacacgaacg cgatatgcac gtcatcgccc
     2161 tgaccggccg cgacggcggc aaaatcgccg ccatactcaa agacaccgac gttttactca
     2221 acgttcccca tcctcgaact gcccgtattc aggaaaacca catcctgctg atacacgcca
     2281 tgtgcgactg tatcgactcc gtactgctgg aaggaatgta acccttttca gacggcatgg
     2341 cgcaaagcaa tgccgtctga aacgcccaag aaaggaagcc cccgatgaaa ctcaaactgc
     2401 acaccgtccg catcctgacc gccgccattt tcagccttgc ccttagcggc tgcgtcagcg
     2461 cagtaatcgg cggtgcggcg gtcggcgcga aatccgccgt cgaccgccgc accaccggcg
     2521 cgcaaaccga cgacaacgta atggcgttgc gtattgaaac caccgcccgc tcctacctac
     2581 gccaaaacaa ccaaaccaag ggctacacgc cccaaatctc cgtcgtcggc tacaaccgcc
     2641 acctgctgct gctcggacaa gtcgccaccg aaggcgaaaa acagttcgtc ggtcagattg
     2701 cacgttccga acaggccgcc gaaggcgtgt acaactacat taccgtcgcc tccctgccgc
     2761 gcactgccgg cgacatcgcc ggcgacactt ggaacacatc caaagtccgc gccacgctgt
     2821 tgggcatcag ccccgccaca caggcgcgcg tcaaaatcgt tacctacggc aacgtaacct
     2881 acgttatggg catcctcacc cccgaagaac aggcgcagat tacccaaaaa gtcagcacca
     2941 ccgtcggcgt acaaaaagtc atcaccctct accaaaacta cgtccaacgc tgactcggca
     3001 atgccgtctg aaccgccttc agacggcatt gcccgacacc ccaaaagcac aatcaaaatg
     3061 gcaaaaaaac cgaacaaacc cttcaggctg acccccaaac tcctgatacg cgtcgtattg
     3121 ctcatctgta tcaccgccat cggcgcattg gcaataggca tcgtcagcac attcaacccg
     3181 aacggcggca aaaccctcca aaccgaaccg caacacaccg acagcccccg cgaaaccgaa
     3241 ttctggctgc caaacggcgt agtcggacaa gatgccgccc aacccgaaca ccaccacgcc
     3301 gcctcatccg aacccgcaca gccggacggc acagacgaaa gcggcagcgg actgccgtcc
     3361 cctgccgcac ccaagaaaaa ccgggtcaaa ccgcaacctg ccgacacagc tcaaaccgac
     3421 aggcagccgg acgacgccgg aacacaagct gaaaacacac tcaaagaaac ccccgtactg
     3481 cccacaaacg tcccccgtcc cgaaccccga aaagaaacac ccgaaaaaca ggcgcagccc
     3541 aaagaaacac ccaaagaaaa agaaacgccc aaagaaaacc ataccaaacc ggacaccccg
     3601 aaaaacacgc cgcccaaacc ccataaagaa attctcgaca acctcttctg acccggcacg
     3661 gcaggcacac ccgcaatcca aggaagcatt atgaacggca tcatcatcaa aacccccgaa
     3721 gaaatcgaaa aaatgcgcga gctgggcaaa ctcgtcgccg aagccctcga ctacatcgga
     3781 caattcgtca aacccggcgt aaccaccgac gaaatcgaca aactcgttta cgactaccac
     3841 gtcaacgtcc aaggcggcta tcccgccccc ctgcactacg gcaacccgcc ctaccccaaa
     3901 tcctgctgca cctccgtcaa ccacgtcatc tgccacggca ttcccgacga caagccgctc
     3961 aaagaaggcg acattatcaa catcgacctc accatcaaaa aagacggctt ccacggcgac
     4021 tccagccgta tgtttaccgt cggcaaagtc tcccccatcg cccaacgcct gatcgacgta
     4081 acccacgcct ccatgatggc gggcatagaa gccgtcaaac ccggcgcgac actgggcgac
     4141 gtaggttacg cctgccaaca ggttgccgaa aacgccggct attccgtcgt acaggaattc
     4201 tgcggacacg gcatcgggcg cggtttccac gaagccccgc aagtgttgca ctacggaaaa
     4261 aaaggacagg gccccgttct aaaaccgggt atgattttta ccgtcgaacc gatgatcaac
     4321 caaggcaaac gccacctgcg tatcctcaac gacggctgga cggtggttac caaagaccgc
     4381 tccctctccg cccaatggga acacgaagtc ttggtgaccg aaaccggcta cgaaatcctc
     4441 accgtcagcc ccgcctccgg caaaccctga aaccggacgt atccgcccca taaaaacaaa
     4501 caatgccgtc tgaaagaaac ggcagatatg atatataata taaaaacagg cttgacccgg
     4561 cacattacga aaacaaagca aatcggaatt tgccccgcaa ccagacaaac ttaaaggaag
     4621 ttttatgaaa atatttgaaa atatagaaga tgttaaagcc atccgtaaaa agaccgggct
     4681 gaaccagata gacttctggg gcaaggtcgg cgttacccag tccggaggat cgcgctacga
     4741 aaccggccgc aaaatgccca aacccgtacg cgaactgctc cgcctcgtcc atatcgaatg
     4801 catcgatttg gcgaaagtca acaaaaaaga tatggaaatc gccgccctgt tgaaaaaaca
     4861 ccatcccgac ctgtatgccg agttgtccaa acagaccaag tccgaaagaa aaaaacaaag
     4921 ttaaaccgca acctccggat gcccgaaggt ttttcatttc cgaaaaacgc aaacaatgcc
     4981 gtctgaaaca ccggacaggc cgccgtatcc cgcctgccgc ccctgcctca aaccgccgaa
     5041 ccgcccgaac ccgccttttt acaaacttta tccaatttcc tgtttatttc aggatacgcc
     5101 gacattagaa tgtcaaacag ctcgaaacgg gcaaactcca catccatcca aaggaataaa
     5161 aatgaaactt ctgaccaccg caatcctgtc ttccgcaatc gcgctcagca gtatggctgc
     5221 cgccgctggc acggacaacc ccactgttgc aaaaaaaacc gtcagctacg tctgccagca
     5281 aggtaaaaaa gtcaaagtaa cctacggctt caacaaacag ggtctgacca catacgcttc
     5341 cgccgtcatc aacggcaaac gcgtgcaaat gcctgtcaat ttggacaaat ccgacaatgt
     5401 ggaaacattc tacggcaaag aaggcggtta tgttttgggt accggcgtga tggatggcaa
     5461 atcctaccgc aaacagccca ttatgattac cgcacctgac aaccaaatcg tcttcaaaga
     5521 ctgttcccca cgttaatcag gcaacaaaaa acagcgtttt cagaaatgaa aacgctgttt
     5581 ttttgaccgt tccattattc acaaaaggga aaaaacgatt acctgccccg tgtatcaaaa
     5641 cctgccctgc cggatgaagg gcataactgg cagggacggc gtcaacacca tatgggggta
     5701 cggcttttct tgaaagattc ggcttaaata tccaatactt tcgcggtata ggcgataatt
     5761 tcatccgccc tttcagggtt ttcgttcaac ttgatgccgt aacccggtac cagctctttc
     5821 agacggtctt cccaagacgg ggcgcgctcg gggaagcatt ggtgcatcag ccggatcatc
     5881 agcggcacag cggtcgatgc gcccggcgac gcgcccagca atgcggcgag tgagccgtcg
     5941 gcgtgggcga caatctccgt accaaactgg agcacgccgc ctttttcgga gtctttttta
     6001 atgatttgga cgcgttgccc tgcggtgatg agttcccagt cgtcggggtt tgcctcgggg
     6061 tagtattcca gcagggaggc gaagcgttct tctttggttt tacgcaattc gcccagcagg
     6121 tatttggtca gcggcatatt cgcccagccg gcgcacagca taggatagag gttgtccata
     6181 tggatggaca gcggcaaatc cataagcgag ccttgcttga ggaagttgga acggaagcct
     6241 gcgtaagggc cgaacataag gtggcgtttg ccgtccacgt tgcgtgtgtc gaggtgcggg
     6301 acggacatcg gcggcgcgcc gacggaagcc tgcccgtaca ctttggcgtt gtgttgttcg
     6361 gcggtttcgg ggttgctgtt gcggaagaac aggccggaca cggggaagcc gccgtagcct
     6421 ttgccttcgg ggatgccgga tttttgcagc agggtcagcg cgccgccgcc cgcgccgagg
     6481 aagaggaagc gggtacggag ggtgagctgc ccgtcggggt tgcgggtatc ggcggttttg
     6541 agcacccacg cgccgtcgga ttcgcgtttg atgtcttcga cgtggcggtt gaactcggtt
     6601 tttacgccct tgccctgcaa atatttcacc atttggcgcg tcagccgtcc gaaatcgaca
     6661 tccgtacctt cggcggagta gttggcggcg acgggttggt tttcgtcccg gccgcgcatc
     6721 atcagcggag cccaatcgga aattttgttc cgatcggtgg aaaattccat attttcaaaa
     6781 agtttttggg ttttaaacgc gtcataacgt ttttgaagat aagaacaatg gtcttcattc
     6841 atcaccaaag acatatgcgg cacggcattg atgaaggaat tgtcttccaa cttgccttcc
     6901 gcgaccagcg tcgcccaaaa ctggcggctg acatgaaact gttcggcaat attgagggcg
     6961 cgcgccggat cgataatccc atttgcaccc aacggcgcat agttcaattc gcacagcgcg
     7021 gaatgccccg tgccggcgtt gttccacgcg tttgacgatt ccaacgccac atcttccaag
     7081 cgttcaatca gggtgatttc ccaagacggt tcgagttctt tgagcaaaac gcccaaagtc
     7141 gcgctcataa tgccgccgcc caccaagaca acgtctgtcg cttcagccat ggtttactcc
     7201 taaaaaacag gcatcttctg cccttatggt tatttgccgt actacaaacg cctgaatcgc
     7261 aaaagcaggg aaaaccgaca atggtgtgtg tccgagtatg ctgtttcggg gttggaatgc
     7321 gttgcaagta tggcttccga caccgcttca ggggcttgta atatgttatc gtgaatgtag
     7381 tggattttac tgggaaatgc aaagtttttc tgtcgcccgc caagtcggga aactgcgaaa
     7441 tgaaaaataa aaatagttat ttatctatat atatcaaatt tttaatagat aaaaaatcaa
     7501 aattgtttat atattaattt ttaaaagatt gtcagcatat tgcgttaagt ttt
//
LOCUS       DAWHSK010000082         7512 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.082, whole genome shotgun sequence.
ACCESSION   DAWHSK010000082 DAWHSK010000000
VERSION     DAWHSK010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7512
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.082"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            156..329
                     /locus_tag="WIC03_001088"
     CDS             156..329
                     /locus_tag="WIC03_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HEZ3883598.1"
                     /translation="MKSERRHWEWCVDYDGEGVVRDYRFTHKVEKDERSVNRDAVGVI
                     RKEAGKSLSQPEK"
     gene            393..1676
                     /gene="hemL"
                     /locus_tag="WIC03_001089"
     CDS             393..1676
                     /gene="hemL"
                     /locus_tag="WIC03_001089"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEZ3883599.1"
                     /translation="MNRNEILFDRAKAVIPGGVNSPVRAFGSVGGVPRFIKKAEGAYV
                     WDENGTRYTDYVGSWGPAIVGHAHPEVIEAVREAALGGLSFGAPTEGEIVIAEEIAKI
                     MPSVERLRLVSSGTEATMTAIRLARGFTGRDKIIKFEGCYHGHSDSLLVKAGSGLLTF
                     GNPSSAGVPADFTKHTLVLEYNNIAQLEEAFAQSGNDIACVILEPFVGNMNLVRPTEA
                     FVKALRELTEKHGAVLIYDEVMTGFRVALGGAQSLHGIMPDLTTMGKVIGGGMPLAAF
                     GGRKDIMECISPLGGVYQAGTLSGNPIAVAAGLKTLEIIRREGFYENLTARTEQLVQG
                     FQTAADAAGIEFAADSVGGMFGLYFAAHAPRNYADMARSNIDAFKRFFHGMLDRGIAF
                     GPSAYEAGFVSAAHTPELIDETVAVAVEVFKAMAA"
     gene            complement(1892..2164)
                     /locus_tag="WIC03_001090"
     CDS             complement(1892..2164)
                     /locus_tag="WIC03_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taxon MazF"
                     /protein_id="HEZ3883600.1"
                     /translation="MPSEAQILHFRRHFYSRLKSKIYTPVIPAQAGIRKVGLVGHEVV
                     LKMKRYRKSDELKEKYSDIDETFEFIKGFIYVIALAFTAYVAIRFF"
     gene            complement(2175..2738)
                     /gene="orn"
                     /locus_tag="WIC03_001091"
     CDS             complement(2175..2738)
                     /gene="orn"
                     /locus_tag="WIC03_001091"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HEZ3883601.1"
                     /translation="MQDKNNLCWLDMEMTGLNPETDRIIEVAMIITDSDLNVLAQSEV
                     YAVHQSDELLDNMDEWNTATHGRTGLTQRVRESSHTEAEVEQKLLDFMSEWVPGRATP
                     MCGNSIHQDRRFMVKYMPKLENYFHYRNLDVSTLKELAKRWNPPVAKSVVKRGSHKAL
                     DDILESIEEMRHYREHFLISAPRAEAQ"
     gene            complement(2756..3643)
                     /gene="prmA"
                     /locus_tag="WIC03_001092"
     CDS             complement(2756..3643)
                     /gene="prmA"
                     /locus_tag="WIC03_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEZ3883602.1"
                     /translation="MPYQQITVNVNDAVAERLADALMEHGALSAAIEDAYAGTQNEQA
                     IFGEPGMPAEQIWQQSKVIALFGEHDEAAAIIQTAAQECGLKDLAYTGETLEDQDWVR
                     LTQAQFDPIRISERLWITPSWHEAPEGTAVNLRLDPGLAFGTGSHPTTRLCLKWLDTQ
                     LKNGESVLDYGCGSGILTIAALKLGAGSAVGVDIDEQAVRAGKDNAEQNNVDAQFFLP
                     DGLPQGQFDVVVANILANPLRMLGEMLAARTKQGGRIVLSGLLDEQAEELGGIYSQWF
                     DLDPAETEESWARLSGTKR"
     gene            complement(3761..5122)
                     /gene="accC"
                     /locus_tag="WIC03_001093"
     CDS             complement(3761..5122)
                     /gene="accC"
                     /locus_tag="WIC03_001093"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEZ3883603.1"
                     /translation="MLKKVLIANRGEIALRVLRACREMGIATVAVHSEADKDSLHVKL
                     ADESVCIGPAASAQSYLNVPAIIAAAEVSCADAVHPGYGFLAENADFAEQVEQSGFTF
                     IGPKPDTIRLMGDKVSAKHAMIAAGVPCVPGSDGALPDDGEEILKIADKVGYPVIIKA
                     SGGGGGRGMRVVEKKEDLLQSVEMTKAEAGAAFGNPMVYMERYLQRPRHVEIQVIADE
                     HGNAVYLAERDCSLQRRHQKVIEEAPAPFITEKERAKIGNACADACKRIGYRGAGTFE
                     FLYEDGEFFFIEMNTRVQVEHPVTELITGVDIVQEQLRIASGLPLQYKQKDIKIEGHA
                     FECRINAEDPYNFIPSPGPIESCHLPGGFGIRVDSHIYQGYRIPPYYDSLIGKICVHG
                     KTREQAMAKMRVALAELAVTGIKTNTPLHRDLFADAGFQKGGVSIHYLEHWLEDRKAK
                     QDK"
     gene            complement(5235..5690)
                     /locus_tag="WIC03_001094"
     CDS             complement(5235..5690)
                     /locus_tag="WIC03_001094"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEZ3883604.1"
                     /translation="MDLRKLKKLIDLVEESGIAEIEVTEGEEKVRITRTIAAAPVYAA
                     PVPAAAPAVTPAAAPVAASAPAAAPAARDLSDAQKSPMVGTFYRAPGPNAAPFVEVGQ
                     QVKAGDTLCIIEAMKLMNEIEAEKSGTVKEILVENGTPVEFGEPLFIIG"
     gene            complement(5672..5929)
                     /locus_tag="WIC03_001095"
     CDS             complement(5672..5929)
                     /locus_tag="WIC03_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase"
                     /protein_id="HEZ3883605.1"
                     /translation="MRKIKLPAHFPQKPPFFDILLDIYRQLRENKHILTVVFHHRKTV
                     SEHHSARFAPLPQAGCFLKNQRNNPPEHRQPLKEQKWICAN"
     gene            6046..7086
                     /gene="queA"
                     /locus_tag="WIC03_001096"
     CDS             6046..7086
                     /gene="queA"
                     /locus_tag="WIC03_001096"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEZ3883606.1"
                     /translation="MDISDFDFTLPEKLIAQHPPEVRGSSRLLVALPDMPLQDRVFGD
                     LPDYVEAGDVLVFNNTKVMKARLFGQKDSGGRIEALIERVLDNHTALAHIRSSKSPKP
                     GMGLVFEGGIRAVMVGREGELFCLRFEGGQTVYELLEQNGHLPLPPYIERAADADDDS
                     RYQTVYAKYQGAVAAPTAGLHFTEELLRRLKDKGAVTAEVTLHVGAGTFQPVRVEKIE
                     EHKMHSEWFEVPSETAAAVEAAKARGNKVWAVGTTSMRALESAARATGRLKAGQGDTD
                     IFITPGYRFNVVDRLVTNFHLPKSTLLMLVSAFSGMGHIRAAYRYAVEREYRFFSYGD
                     AMVLGRNEGVVR"
BASE COUNT         1557 a         1882 c         2240 g         1833 t
ORIGIN      
        1 agacggaaga gggaatcgca caaacggttg ttaaaggcaa aacgacccgg gcggaggtag
       61 aggcacgttt cgggaagcgc aaccctttcg gttgttatgc ctatcatgaa gtcagcctgc
      121 cgatttataa ttttttgccg accaatttca tttatatgaa atcagaacgc cggcattggg
      181 agtggtgtgt ggattacgac ggggagggag ttgtccggga ctaccgcttt acacataaag
      241 tggaaaaaga cgaacgttcc gtcaaccggg atgcggttgg cgtgatccgc aaagaagcgg
      301 gcaaatcctt gtcgcaacct gaaaaatgat aaaatggggc tttctgcttc caagcccgaa
      361 acctgccgtt caggcggcat ttgaggataa atatgaaccg taacgaaatt ttattcgacc
      421 gcgccaaggc ggtcatcccc ggcggtgtga attcgccagt ccgcgcattc ggcagcgtcg
      481 gcggcgtacc gcgcttcatc aaaaaagccg aaggcgcgta tgtttgggat gaaaacggca
      541 cgcgctacac cgactatgtc ggctcgtggg ggcctgcgat tgtcggccac gcgcaccccg
      601 aagtcatcga agccgtgcgc gaagccgcgt tgggcggttt gtcgttcggc gcgcctaccg
      661 agggcgaaat cgtcatcgcc gaagaaatcg ccaaaatcat gccgtccgtc gaacggctgc
      721 gcctcgtcag ctccggtacc gaagcgacga tgaccgccat ccgtttggca cgcggtttta
      781 ccggtcgcga caaaatcatc aagtttgaag gctgctacca cgggcattcc gacagcctgc
      841 tggtgaaagc cggcagcggc ctgctgacgt tcggcaatcc ttcttccgcc ggtgtgcctg
      901 ccgactttac caaacacact ttagtccttg agtacaacaa catcgcccaa ttggaagaag
      961 cctttgctca aagcggcaat gacattgcct gtgtgatttt ggagcctttt gtcggcaata
     1021 tgaaccttgt ccgcccgacc gaagccttcg tcaaagccct gcgcgaattg accgaaaaac
     1081 acggcgcggt gttgatttac gacgaagtga tgaccggttt ccgcgtcgcg ctcggcggcg
     1141 cacaatcgct gcacggcatc atgcccgacc tgaccacgat gggcaaagtc atcggcggcg
     1201 gtatgccgct tgccgcgttc ggcgggcgca aagacattat ggaatgtatt tccccactgg
     1261 gcggcgtgta tcaggcaggt acattatcag gcaacccgat tgccgtcgcc gccggtttga
     1321 aaacgctgga aatcatccgc cgcgaagggt tttatgaaaa cctgacggcg cgtaccgaac
     1381 agttggttca aggttttcag acggcagcgg atgcggcggg catcgagttt gccgccgaca
     1441 gcgtgggcgg tatgttcggt ctgtatttcg ccgcacacgc gccgcgaaac tatgccgata
     1501 tggcgcgttc caatattgac gctttcaaac gcttcttcca cggtatgctc gaccgcggca
     1561 ttgccttcgg cccgtccgct tatgaagcag gtttcgtttc tgccgcgcat acgcccgagc
     1621 tgattgacga aacggttgcg gttgcggttg aagtgttcaa ggcgatggct gcatgatgtt
     1681 ttgacggaca gagtttctct gttcgatttg tttggcagat tgaagtaaga atgcacaccg
     1741 ccgtcattcc cgcgcaggcg ggaatccaga cctttcagtt tctgtaatga ttgaaaataa
     1801 cgggccgaaa tcagccgcgc ccggcaggaa gccgccgcca cctgaaacaa tgccgtctga
     1861 agccttcaga cggcatttta tcggaagcgc atcagaaaaa acggatagcg acatatgcgg
     1921 taaaagcaag ggcaattaca tagataaacc cctttataaa ttcaaaagtc tcatctatat
     1981 cgctatattt ttctttcagt tcgtcagatt ttcgataacg tttcattttc aagaccactt
     2041 catgaccaac aagcccgacc ttccggattc ccgcctgcgc gggaatgacg ggcgtgtata
     2101 tttttgattt caatctgctg taaaaatgcc gtctgaaatg caggatttgc gcttcagacg
     2161 gcattttttg tttcttattg cgcttcggct ctcggggcgg aaatcagaaa gtgttcgcgg
     2221 tagtggcgca tttcttcgat gctctccaaa atgtcgtcca atgccttgtg cgaaccgcgt
     2281 ttgacgacgc ttttggcaac gggcggattc cagcgtttgg caagctcttt cagtgtggaa
     2341 acgtcaaggt tgcggtagtg gaagtagttt tccagtttcg gcatatattt gaccataaaa
     2401 cgccggtctt ggtggatgga attgccgcac atcggcgtgg cgcgtccggg tacccattcc
     2461 gacataaagt ccagcagttt ctgttcgact tcggcttcgg tatgcgacga ttcgcgtacg
     2521 cgctgtgtca gccccgtcct gccgtgtgtg gcggtgttcc actcgtccat attgtcgagc
     2581 agctcgtcac tttggtggac ggcgtaaact tcggattgcg ccaatacatt caaatccgag
     2641 tcggtaataa tcatcgcgac ttcgataatg cggtcggttt cgggattaag ccccgtcatt
     2701 tccatatcga gccagcagag gttgtttttg tcctgcacgg tgtttccttt ccgtttcagc
     2761 gttttgtccc gctcaagcgc gcccagcttt cttcggtttc cgccgggtcg agatcgaacc
     2821 attggctgta aatgccgccg agttcttcgg cttgttcgtc caacaaaccg gataacacga
     2881 tgcggccgcc ttgtttggtg cgggcggcga gcatttcgcc gagcatacgt aaagggttgg
     2941 cgaggatgtt ggcgacaact acgtcgaatt gcccttgagg cagaccgtcg ggcaggaaga
     3001 attgtgcatc gacgttgttc tgttcggcgt tgtccttgcc ggcgcggacg gcttgttcgt
     3061 caatatccac gccgacggca gaacctgcgc cgagtttgag ggcggcgatg gtcaggatgc
     3121 ccgaaccgca gccgtagtcg aggacgcttt cgccgttttt gagttgcgta tccagccatt
     3181 tgaggcagag gcgcgtggtc gggtggctgc cggtgccgaa ggcgaggccg ggatcgaggc
     3241 gtaggttgac ggcagtgcct tcgggggctt cgtgccaaga gggggtaatc cacaggcgtt
     3301 cggaaattcg gatggggtcg aattgcgctt gggtcaggcg tacccagtct tggtcttcga
     3361 gggtttcgcc ggtgtatgcc aagtctttta acccgcattc ttgtgcggcg gtttggatga
     3421 tggcggcggc ttcgtcgtgt tcgccgaaca gggcgatgac tttgctctgc tgccagattt
     3481 gttcggcggg cataccgggt tcgccgaaaa tcgcctgttc gttttgcgtg ccggcgtagg
     3541 catcttcgat ggcggcggag agtgcgccgt gttccatcag cgcgtcggcg aggcgttcgg
     3601 cgacggcatc gttgacgttg acggtgattt gttggtaggg cataacgggc tttcttaaca
     3661 gaccttcgga atagggtcgt actgtataag gggatgggtg tgtaacggaa atgccgtctg
     3721 aaaacggaca aactgtttca gacggcatat cggcggcggt ttacttgtcc tgtttggctt
     3781 tgcgatcttc cagccagtgt tccaaatagt ggatgctgac gccgcctttt tggaaacccg
     3841 catcggcgaa caggtcgcgg tgaagcggcg tattggtttt gatgccggtt accgccagct
     3901 cggcgagtgc gacgcgcatt ttcgccattg cctgttcacg cgttttgccg tgtacgcaga
     3961 ttttgccgat caggctgtcg tagtacggtg ggatgcggta gccttggtaa atgtggctgt
     4021 ccacgcggat accgaagcca ccgggcaggt ggcagctttc aatcgggcct gggctgggga
     4081 tgaagttgta cgggtcttcg gcgttgatac ggcactcgaa agcgtggcct tcgattttga
     4141 tgtctttctg tttgtattgc agcggcaggc cggatgcgat gcggagttgc tcctgcacga
     4201 tgtccacgcc ggtgatgagt tcggtaaccg gatgctcaac ctgaacgcgc gtattcatct
     4261 cgataaagaa aaattcgccg tcttcgtaca ggaattcaaa cgtacctgcg ccacgatagc
     4321 cgatgcgttt gcaggcgtcg gcacaggcgt tgccgatttt ggcgcgttct ttttcagtga
     4381 tgaacggagc cggtgcttcc tcgatgactt tttggtggcg gcgttgcagc gaacagtcgc
     4441 gctcggcaag atatacggcg ttgccgtgtt cgtcggcaat cacttggatt tcgacgtggc
     4501 gcggacgttg cagatagcgt tccatgtaaa ccatcgggtt gccgaatgcc gcgcctgctt
     4561 ctgccttggt catttcgaca gattggagga ggtcttcttt tttctcgacc acgcgcatac
     4621 cgcgaccgcc gccgccgcca gaggctttga taatgacggg ataaccgact ttatcggcga
     4681 ttttgaggat ttcttcgccg tcgtcgggca atgcgccgtc agaaccgggg acgcagggta
     4741 cgcctgccgc tatcatcgcg tgtttggcgg agactttgtc gcccatcagg cggatggtgt
     4801 cgggtttcgg gccgataaag gtaaagccgg actgctcgac ctgttcggcg aaatcggcgt
     4861 tttcggcaag gaaaccgtag cccggatgga cagcgtccgc gcagcttact tcggcggcgg
     4921 cgataatggc ggggacgtta aggtagcttt gcgcggaagc ggcagggccg atgcacacgg
     4981 attcgtcggc gagtttgacg tgcaggctgt ctttgtcggc ctcggaatgc acggcgacgg
     5041 tggcaatgcc catttcgcgg caggcacgga gtacgcgtaa tgcgatttcg cctcggttgg
     5101 cgattaaaac ttttttcagc atgatgactg ttcctgtggt tccggcaacc cgtccgtaaa
     5161 aaaaaggtgc gggaacgctg aagggggaaa gcatttcaga cggcatcgga agtttatgcc
     5221 gtctgaaaac aggattatcc gatgatgaag agcggttcgc cgaattcgac gggcgtaccg
     5281 ttttcgacca aaatttcttt gaccgtgccg gatttttcgg cttcgatttc gttcatcagc
     5341 ttcatcgctt cgatgatgca cagcgtgtcg ccggctttaa cctgttggcc gacttcgaca
     5401 aaaggcgcgg cattcgggcc gggtgcgcgg tagaacgtgc cgaccatagg tgatttttgc
     5461 gcgtcggaca aatcgcgggc ggcaggtgcg gcggcaggcg cggatgccgc aacgggtgcg
     5521 gcggcaggcg ttacggccgg cgcggcggca ggtacgggcg cggcgtaaac gggtgcggcg
     5581 gcgatggttc gggtgatgcg gactttttcc tcgccttcgg ttacttcgat ttcggcgata
     5641 cccgattctt caaccaaatc aatcagtttt tttaatttgc gcaaatccat ttctgttcct
     5701 ttaaaggctg ccggtgttcc ggcgggttgt tgcgttggtt tttcagaaaa cagccggctt
     5761 gcggcaacgg cgcaaaccgg gcggaatggt gttcggatac ggttttcctg tggtggaaaa
     5821 caaccgtgag aatgtgttta ttttcccgaa gttgtcggta aatgtccagt aaaatatcaa
     5881 aaaacggcgg tttttggggg aaatgtgcag gaagtttgat tttgcgcaca agatgttcca
     5941 ggttaagagt gtttattcta atctgttggt ttttcggaca aagatgccgt ctgaaaaagg
     6001 gctaaagtgc gtataatggc ggcttgcccg aacgagagtg taaaaatgga tatttcagat
     6061 tttgatttta ccctgcccga aaagctgatt gcccagcatc cgcccgaagt gcgcggcagc
     6121 agccgccttt tggttgcgtt gcccgatatg ccgctgcaag accgggtgtt cggcgatttg
     6181 ccggattatg tcgaggcggg cgacgttttg gtgttcaaca acaccaaagt catgaaggcg
     6241 cggctgtttg ggcagaaaga cagcggcggc aggatcgaag ccctgattga gcgcgtgttg
     6301 gacaaccata ccgcactggc gcacatccgt tcgtccaagt cccccaagcc cggtatgggg
     6361 ctggtgtttg aaggcggcat ccgtgccgtg atggtcgggc gtgagggcga actgttctgc
     6421 ctgcgttttg aaggcggtca aaccgtttac gaacttttgg aacagaacgg acacctgccc
     6481 ctgccgcctt atatcgaacg cgccgccgac gcggacgacg acagccgtta tcaaaccgtt
     6541 tatgccaaat atcagggtgc ggtcgccgcg ccgacggcgg gtctgcattt tacggaagaa
     6601 cttttgcgcc gcctgaaaga caaaggcgca gtaaccgcag aagtaaccct gcacgtcggt
     6661 gcgggaacgt tccagcccgt gcgtgtggaa aaaatcgaag aacacaaaat gcacagcgaa
     6721 tggtttgaag tgccgtctga aaccgccgcc gccgttgagg cggcaaaagc acgggggaac
     6781 aaagtctggg cggtcggcac gacttccatg cgcgccctcg aatctgccgc gcgtgcgacg
     6841 ggtcgtctga aagcggggca gggcgacacc gatattttca tcacgccggg ctaccgtttt
     6901 aatgttgtcg acaggctggt taccaatttt catttgccga aatcgacgct gctgatgctt
     6961 gtcagcgcgt tttcgggtat gggtcatatc cgcgccgcgt atcgttatgc ggtcgaacgc
     7021 gaataccgtt tcttcagcta cggcgatgcg atggttttgg ggcggaacga aggggtggta
     7081 cgttagaaca gccccaagtc tattggggga agacaagtgc cgtctgaacg gacgttcgga
     7141 cggcattctt ttgttgattt gaatgaaagg aactgtatga catagattca ggcgcgagag
     7201 gcgagaaact cagtcattgt tgatgacgcg caatacgtta aaattcattc cgttttggtc
     7261 gtataaacat attacatacg ccattttttt gtattcaagg ctgcctgaaa acagtgggca
     7321 agaatatcta acccaacgaa aagctgaaaa agcaacaaaa aataatctgc accttagtcg
     7381 gttggttagt ccaaccttag gggtgcagac cattcaatct ttcaggctgc ctttgtttgt
     7441 ttataagata aatttttagc cgaacacttt ttccaagtgt gcctgataat ctgccaagta
     7501 tttttccact tg
//
LOCUS       DAWHSK010000083         7507 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.083, whole genome shotgun sequence.
ACCESSION   DAWHSK010000083 DAWHSK010000000
VERSION     DAWHSK010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7507
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.083"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(8..1396)
                     /gene="fumC"
                     /locus_tag="WIC03_001097"
     CDS             complement(8..1396)
                     /gene="fumC"
                     /locus_tag="WIC03_001097"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEZ3883607.1"
                     /translation="MSTRTEHDTMGNVEVPSEAYWGAQTQRSRNNFKIGGETLPQPLI
                     YALALVKKAAAATNVSLGRIKPEQADLITQAADDVLNGKLDGQFPLVVWQTGSGTQSN
                     MNMNEVLANRANEIAGTGLAAYQPVHPNDHVNHAQSTNDAFPTAIHVAAAIEINRHLI
                     PAVKALRDTLDKKAQAFAPIVKIGRTHLQDATPLTLGQEFSGYVSQLDHGLGRLNDAL
                     KDLYELALGGTAVGTGLNSHPEYAEKAAAKLAELSGLPFVSAPNKFEALGGRDAAVAA
                     SGALKTLAASLNKIANDIRWLASGPRCGLGEIKIPENEPGSSIMPGKVNPTQCEAMTM
                     VCCQVFGNDVTIGMAGASGNFELNVYMPVIAYNLLQSIRLLGDACNSFNEHCAIGIEP
                     VPEKIDYFLHHSLMLVTALNRKIGYENAAKVAKTAYKNNKSLRETAVELGLLTGEEFD
                     ELVVPADMVHPR"
     gene            complement(1540..3519)
                     /gene="tkt"
                     /locus_tag="WIC03_001098"
     CDS             complement(1540..3519)
                     /gene="tkt"
                     /locus_tag="WIC03_001098"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEZ3883608.1"
                     /translation="MSQLANAIRFLSADAVQKANSGHPGAPMGMAEMAETLWTKFLNH
                     NPANPKFYNRDRFVLSNGHASMLLYSLLHLTGYNLSIEDLKNFRQLHSKTPGHPEYGY
                     TDGVETTTGPLGQGIANAVGMALAEKILAAEFNKDGLNIVDHYTYVFMGDGCLMEGVS
                     HEACSLAGTLGLGKLIVLYDDNNISIDGKVDGWFTENIPQRFESYGWHVVPNVNGHDT
                     AAIQAAIEAARAETGKPSIICCKTLIGKGSANKESSHKTHGAPLGADEIEATRKHLGW
                     TYPAFEIPQEIYDAWNAKEKGAKLEAEWNELFAQYQAKYPAEAAEFVRRMDKKLPDNF
                     DEYVQAALKEVCAKAETIATRKASQNSIEILAKELPELVGGSADLTPSNLTDWSNSVS
                     VTRDKGGNYIHYGVREFGMGAIMNGLVLHGGVKPFGATFLMFSEYERNALRMAALMKI
                     NPVFVFTHDSIGLGEDGPTHQPIEQTATLRLIPNMDVWRPCDTAESLVAWAEAVKAAD
                     HPSCLIFSRQNLKFQARSEQQLDDIKRGGYIISEAQGNAQAVIIATGSEVELALEAQK
                     ALAAQNIAVRVVSMPSTNVFDRQDAAYQAAVLPESLPRIAVEAGHADGWYKYVGLNGA
                     VVGINRFGESAPADLLFKAFGFTVDNVVDTVKSVL"
     gene            4047..5543
                     /gene="ccoG"
                     /locus_tag="WIC03_001099"
     CDS             4047..5543
                     /gene="ccoG"
                     /locus_tag="WIC03_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HEZ3883609.1"
                     /translation="MTTENQAGSPASGIGTSEQTKAAPKAKKTFDPRASVIQIHPEGE
                     RIHPKKAEGRFAKLRIAAVLATQFVFYVIPWFNWSGRQAVVFNIPERHFFIFGLSLGV
                     GDLIYLALLLMICAFGLFWWTTIAGRLWCGYSCPQTVYTEIMLWIDNLVEGDRNKRLK
                     LEKSPWNFTKIRIKATKYLLIFLVCAWTGITFAGWFVPIRQFVPDLFTGAAGGGAMFA
                     TAFYGFMTFFFAHIMREKVCLHMCPYARFQSAMFDKDTLIVSYDAERGEPRGARKKTV
                     NKEEAGLGDCINCAMCVQVCPVGIDIRNGLQYQCIGCAACIDACDEIMDKMGYPRGLI
                     RYTTESALEHEYSEKDIKKRLLRPRVAGYGAVLALVIIAFLAGLSTRKMVEVDILKDR
                     GVLVRENAKGWLENAYSLRIINKSEKEQLITASVKGFDEIALTGLPEGGIKVAPRETV
                     TLPVQVSTIPEYADKGSHPIEFTFQYRESGAPDGKPVVLEEDATFIGE"
     gene            5546..6094
                     /locus_tag="WIC03_001100"
     CDS             5546..6094
                     /locus_tag="WIC03_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HEZ3883610.1"
                     /translation="MSQNTPIKPWYKHVWPWILMAGPIFVVIASVAMFFVAQQHATDL
                     VTDDYYKDGKHIDIQLHRDEEAVRRHIGVQVLISPDMNAAKVFVGGEFDGKQPLNLLL
                     MHPTRKADDQTVALKPVGSAQNGRAEYEAVFKTLSPTNHWYVRVEDAAGVWRVENKWI
                     TSQGNAVDLTPMDKLFNNTESK"
     gene            complement(6154..7329)
                     /locus_tag="WIC03_001101"
     CDS             complement(6154..7329)
                     /locus_tag="WIC03_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEZ3883611.1"
                     /translation="MQNIFDPLVIRGKSLTPIVQGGMGVGVSASGLSSAVARENGIGT
                     IASVDLRHLHEDLLAESQINPSEEKYTSLNCTALDREIQKAKSASEGKGLIAVNVMKA
                     VKDHAAYVRQACESGADAVVMGAGLPLDLPEMTEGYHKDVALLPILSESRGINIVLKR
                     WMKKGILPDAIVVEHPAHAAGHLGASTVEGVNDAKFDFKRVIEETFEVFKSLGLESEK
                     IPLILAGGMANFEKVKTALKNWGASAVQIGTAFAVTEEGDAHLNFKKTLAGAETEKVV
                     EFMSVAGLPARGVRTKFLDSYIKRESKLQTNAKADPRRCTQGLNCLTSCGLRDGLSKA
                     GQFCIDIQLAAAFRGEVDKGLFFRGKDPLPFGNAIRTVRETIQYLLTGSEPVATVGR"
BASE COUNT         1647 a         1865 c         2139 g         1856 t
ORIGIN      
        1 ggaaagatta gcgcggatga accatatcgg caggaacgac cagttcgtca aattcttcgc
       61 ccgtcagcaa gcccaactca acggcggttt cgcgcaacga tttgttgttt ttgtaggcgg
      121 ttttggcgac tttggcggcg ttttcgtaac cgattttacg gtttaacgcg gtaacgagca
      181 tcagggaatg gtgcaggaaa tagtcgattt tttccggtac gggttcgatg ccgatggcgc
      241 agtgttcgtt gaagctgttg cacgcgtcgc ccaagaggcg gatggattgc aagaggttgt
      301 aggcgataac gggcatatag acgttcagct cgaaattgcc cgacgcgccc gccatgccga
      361 tggtaacgtc gttgccgaac acttggcagc acaccatggt catcgcttcg cattgggtcg
      421 ggttgacttt gcccggcatg atggacgaac ccggctcgtt ttcggggatt ttgatttcgc
      481 ccaaaccgca gcgcgggccg cttgccagcc agcggatgtc gttggcgatt ttattcaggc
      541 ttgccgccag cgttttcaat gcgcccgaag cggcaacggc ggcatcgcgt ccgcccaagg
      601 cttcaaattt gttcggcgcg ctgacaaacg gcaagccgga caattcggcg agtttggcgg
      661 cggctttttc ggcgtattcg ggatggctgt tcaaacccgt gccgaccgcc gtaccgccca
      721 aagcaagttc atacaagtct ttaagcgcgt cgttcagacg gcctaagccg tggtcgagct
      781 gggaaacgta gccggaaaat tcttggccta aagtcagcgg cgtcgcgtcc tgcaagtggg
      841 tgcggccgat tttgacgata ggggcgaagg cttgggcttt tttgtccaag gtgtcgcgca
      901 gggcttttac ggcggggatg aggtggcggt tgatttcaat cgcggcggca acgtggatgg
      961 cggtcgggaa tgcgtcgttg gtcgattgcg cgtggttcac atggtcgttg ggatggacgg
     1021 gctgatacgc cgccaaaccc gtaccggcga tttcgttggc gcggttcgcc agcacttcgt
     1081 tcatgttcat attggactgc gtgccggagc cggtctgcca aaccactaaa ggaaactgtc
     1141 cgtcgagctt gccgttcaac acatcatccg ccgcctgcgt aatcaaatcc gcctgttccg
     1201 gcttaatcct gccgagggaa acattggtcg cggcggcggc ttttttcacc agcgccaaag
     1261 cataaatcaa cggctgcggc agggtttcgc cgccgatttt gaaattgttg cggctgcgct
     1321 gggtctgcgc gccccaatag gcttcggacg ggacttcgac attgcccatg gtgtcgtgtt
     1381 cggtacgggt gctcatgcgt ttctcctttt gaaatgtgaa taagagtgat tcgcaaatat
     1441 tataatggag attggcggaa tgaggaagca ttaaggctgg agggcaaaaa aatgccgtct
     1501 gaaaagcgtt cagacggcat ttgatgcggc ggctgcggtt tacagcacgg atttcactgt
     1561 atcgaccaca ttgtccacgg taaagccgaa tgctttgaag agtaaatcgg caggggcgga
     1621 ttcgccgaag cggttgatgc cgacgactgc gccgttcagt ccgacatatt tgtaccagcc
     1681 gtcggcgtgg ccggcttcta cggcgatgcg cggcaggctt tcgggcagga cggcggcttg
     1741 ataggcggcg tcttggcggt cgaatacgtt ggtggacggc atggaaacga cgcgcacggc
     1801 gatgttttgc gcggcgaggg ctttttgcgc ttccaaagcc agctcgactt ctgagccggt
     1861 ggcaatgatg acggcttggg cgttgccttg ggcttcgctg atgatgtagc cgccgcgttt
     1921 gatgtcgtcc agttgttgtt cgctgcgcgc ttggaatttc aggttttgac ggctgaaaat
     1981 caggcaggac gggtgatcgg cggctttgac ggcttctgcc caagcgacca aggattcggc
     2041 ggtgtcgcac ggccgccata cgtccatatt cggaatcagg cgcagggtgg cggtttgctc
     2101 aatcggctgg tgggtcgggc cgtcttcgcc caaaccgatg gaatcgtggg tgaacacaaa
     2161 tacagggttg attttcatca gtgccgccat acgcagggcg ttgcgctcgt attcgctgaa
     2221 catcaggaaa gtcgcgccga agggttttac gccgccgtgc aataccaaac cgttcataat
     2281 cgcgcccata ccgaactcgc gcacgccgta gtggatgtag ttgccgcctt tgtcgcgggt
     2341 aacggaaacg ctgtttgacc agtcggtcag gttggacggg gtcaggtcgg cagagccgcc
     2401 caccagttcg ggcagctctt ttgccaagat ttcgatgctg ttttggctgg ctttgcgggt
     2461 ggcgatggtt tcggctttgg cgcacacttc tttcaatgcg gcttgaacgt attcatcgaa
     2521 attgtccggc agttttttat ccatacggcg cacgaattct gcggcttcgg caggatattt
     2581 ggcttgatat tgcgcgaaga gttcgttcca ttcggcttcc agtttcgctc ccttttcttt
     2641 ggcattccac gcatcgtaaa tttcttgcgg gatttcaaag gcagggtaag tccagcccaa
     2701 atgtttgcgc gtggcttcga tttcgtccgc gcccaaaggt gcgccgtggg ttttgtggct
     2761 gctttcttta ttggcactgc ctttgccgat taaggtttta cagcagatga tggacggttt
     2821 gccggtttcg gcacgtgcgg cttcgatggc ggcttgaatg gcggcggtgt catgaccgtt
     2881 tacgttagga acgacgtgcc agccgtagct ttcaaagcgt tgcgggatgt tttcggtaaa
     2941 ccagccgtcc actttaccat caatggaaat attgttgtca tcatataaaa caatcagttt
     3001 gcccaagccc aaagtgccgg cgagtgagca ggcttcgtgc gatacgcctt ccatcagaca
     3061 gccgtcgccc ataaagacgt aggtgtaatg atcgacgatg ttcaaaccgt ctttattaaa
     3121 ttcggcggca aggatttttt ctgccaatgc catacccacc gcgttggcaa tcccttgccc
     3181 caacgggccg gtcgtggttt ccacgccgtc ggtgtagccg tattcgggat ggccgggggt
     3241 tttgctgtgc agttggcgga agtttttcaa gtcttcaatg cttaggttgt agccggtcag
     3301 gtgcagcagg ctgtacaaca gcatagacgc gtggccgttg gagaggacga agcggtcgcg
     3361 gttgtagaat ttggggttgg cggggttgtg attgaggaat ttcgtccaca atgtttccgc
     3421 catttccgcc atacccatag gcgcgccggg gtggccggaa ttggcttttt gaacggcatc
     3481 ggccgagagg aagcggattg cgtttgccag ttgagacatt ttgtattttc cttgctggtg
     3541 tttcagataa gtggataatc ggaaagcgtt gattatcgcc cgattcgctt atgctttcaa
     3601 gaaaagggcg gacgcggggg aaggcggcgg caggagaccg gtggggaagg attttcgatt
     3661 tgcgggcgaa gcctgccatt attccttttg aaataaaaag ttatagattg tgtgccggat
     3721 tgtcgatagc gtttgtttat gagcttgcgc cgtcggttct gccgatatgg gggtgtcggt
     3781 tttttttagt ctttttttta accgtattcg gattttgttc gggcggtaag gtaaaatcca
     3841 ggcgttttga tgcggatggg aaatgtatcc gccccataca tccggacggc gcataaagtt
     3901 gtacaatagc ggaaatatat tttgggaaaa acggattttc tcaaaacttg aaacacaacg
     3961 ccttaaaaaa caagaaaaat gacgtctgaa aagcaaacgg cggcggtaac gagcaaacca
     4021 tcaatcaaaa atctaaggaa tgcagaatga ccacggaaaa ccaagccggc agtccggcat
     4081 ccggaatcgg cacgtccgaa caaacaaaag ccgcgccgaa agcaaaaaaa acattcgatc
     4141 cgcgagccag cgtgattcaa atccatcccg aaggcgaacg catccatccg aaaaaggcgg
     4201 aaggacggtt cgccaaactc cgtatcgccg ccgtattggc gacgcagttt gtgttttacg
     4261 tcattccgtg gttcaactgg agcggcaggc aggccgtcgt tttcaatatc cccgaacggc
     4321 atttcttcat tttcggattg tcgttggggg tgggcgattt gatttacctt gccttgctgc
     4381 tgatgatttg cgccttcggg ctgttttggt ggacgacgat tgctgggcga ctgtggtgcg
     4441 gctattcctg cccgcaaacg gtttacaccg aaattatgct gtggatcgac aacctggtcg
     4501 aaggcgatag aaacaaacgg ctgaaactgg aaaaatcgcc gtggaatttc actaaaatcc
     4561 gcatcaaagc caccaaatac ctgttgattt tccttgtctg tgcgtggacg ggcatcacgt
     4621 ttgcaggctg gtttgtccct atccgccagt tcgttcccga tttattcact ggagcagcag
     4681 gcggcggcgc gatgtttgcc acagcgtttt acggctttat gaccttcttc ttcgcccaca
     4741 ttatgcgcga aaaagtatgc ctgcatatgt gtccgtatgc acgtttccaa agcgcgatgt
     4801 tcgacaagga cacgctgatt gtttcttatg acgcggaacg cggcgaaccg cgcggcgcgc
     4861 gcaagaaaac ggtcaataag gaagaggcgg gtttgggcga ctgcatcaac tgtgcgatgt
     4921 gcgtccaagt ctgccccgtc ggcatcgaca tccgcaacgg tctgcaatac caatgtatcg
     4981 gctgcgccgc ctgtatcgac gcgtgcgatg agattatgga caaaatgggc tatccgcgcg
     5041 gattaatccg ttatacgacc gaaagcgcgc tggaacacga atattctgaa aaagacatta
     5101 aaaaacggct gctcagaccg cgcgtggcag gttacggcgc ggtgttggcg ttagttatca
     5161 ttgccttcct ggccggtttg tccacgcgca aaatggtcga ggtcgatatt ttgaaagacc
     5221 gtggcgtact ggtgcgcgaa aacgccaaag gctggctgga aaacgcatac agcctgcgta
     5281 tcatcaacaa aagtgaaaaa gaacagctca ttaccgcaag tgtcaaaggc tttgacgaaa
     5341 tcgccctgac cgggctgccc gaaggcggta taaaggttgc cccgcgcgaa acggtaaccc
     5401 ttcccgtcca agtgtccacc attccggaat acgcggacaa aggcagccac cctatcgaat
     5461 ttaccttcca ataccgcgaa agcggcgcgc ccgacggcaa gccggtcgtc ttggaagaag
     5521 atgcaacctt tatcggagaa taaccgtgtc tcaaaacact ccaatcaaac cttggtacaa
     5581 acacgtctgg ccgtggatct tgatggcggg gccgattttt gtcgtcatcg ccagcgtcgc
     5641 tatgtttttt gtcgcacagc agcacgcgac agatttggtt acggacgatt attataaaga
     5701 cggcaaacat atcgacatcc agcttcatcg ggatgaagag gctgtcagac ggcatatcgg
     5761 ggtgcaggtt ctcatttccc ccgatatgaa tgcggcaaaa gtgtttgtcg gcggcgagtt
     5821 tgacggcaaa cagcctttga acctgctgct gatgcacccg acccgcaagg cggacgatca
     5881 aaccgtcgcc ctcaagcccg tcggcagcgc gcagaacggc agggcggaat atgaggcggt
     5941 gttcaaaacc ctttcgccga ccaaccactg gtatgtgcgc gtggaggacg cggcaggcgt
     6001 gtggcgcgtc gagaacaaat ggattaccag ccaaggcaat gcggtggatc tgaccccgat
     6061 ggataaactt ttcaataata ctgaaagcaa ataaagagat gccgtctgaa aattttcaga
     6121 cggcattttg atacagtatc ggctgttttg acatcagcgt cctactgttg caacaggttc
     6181 actccccgtc agcagatatt gtatcgtctc gcggacggtg cggatggcat tgccgaaggg
     6241 cagcgggtct ttacctctga agaacaggcc tttatctact tctccacgga atgcggcggc
     6301 aagctggata tcaatgcaga attgtccggc tttggaaagc ccgtcgcgca gaccgcaact
     6361 ggttaggcag tttaaacctt gggtacagcg gcgcgggtcg gctttggcgt ttgtctgaag
     6421 tttgctttca cgcttgatgt agctgtctag gaatttggtg cggacaccgc gcgccggcaa
     6481 accggcaaca gacataaatt cgactacttt ttcagtttcc gcaccggcga gcgttttttt
     6541 gaagttaagg tgtgcatctc cttcttcggt aacggcaaaa gccgtaccga tttgaacggc
     6601 agaggctccc cagttcttta gggcggtttt gactttttca aaatttgcca tgcctcccgc
     6661 aagaataagc gggatttttt cgctttccag ccctaaactt ttgaaaactt caaacgtttc
     6721 ctcaatcacg cgtttgaagt cgaacttggc atcgtttacg ccttcaacgg ttgatgcacc
     6781 caaatgtccg gccgcgtggg caggatgttc gactacaatc gcatcgggca atatgccttt
     6841 tttcatccaa cgtttcaaga cgatattaat accgcgcgat tcggacagaa tcggcagcag
     6901 cgcgacatct ttatgatagc cctcggtcat ttccggcagg tctaaaggca gacctgcgcc
     6961 catcacgacc gcatccgccc ctgattcgca agcctggcgg acatacgcgg cgtggtcttt
     7021 gactgccttc atcacgttga ccgcaatcag tccttttccc tctgaagcgc ttttggcttt
     7081 ttggatttcc ctgtctaatg cggtacagtt caaagatgta tatttctctt cactcggatt
     7141 gatttgtgat tcggcgagta ggtcttcgtg aaggtggcgc aaatccacac tggcaatcgt
     7201 tccgataccg ttttcacgtg ccaccgcgct ggataaaccc gatgcggaaa caccgacccc
     7261 cataccgcct tgcacgatgg gggtaaggga ttttccacga ataaccaaag ggtcaaaaat
     7321 attttgcatc agtttctccg agtatacgaa tcagtcaggc tcatgaaaaa tggttctaac
     7381 tgttttagaa ctattgctca aatttggtat tatactctaa atatcgcggc ggtaaacaag
     7441 aaagcagccg gagcttcggg catattctgt tacgcctgtt tcaatcggga gagaaaatat
     7501 tatagtg
//
LOCUS       DAWHSK010000084         7418 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.084, whole genome shotgun sequence.
ACCESSION   DAWHSK010000084 DAWHSK010000000
VERSION     DAWHSK010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7418
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.084"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(35..1414)
                     /gene="norM"
                     /locus_tag="WIC03_001102"
     CDS             complement(35..1414)
                     /gene="norM"
                     /locus_tag="WIC03_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter NorM"
                     /protein_id="HEZ3883612.1"
                     /translation="MLLDLNRFSFSVFLKEVRLLTALALPMLLAQVAQVGIGFVDTVM
                     AGGAGKEDLAAVALGSSAFATVYITFMGIMAALNPMIAQLYGAGKTDEVGETGRQGIW
                     FGLFLGVFGMVLMWAAITPFRNWLTLSDYVEGTMAQYMLFTSLAMPAAMVHRALHAYA
                     SSLNRPRLIMLVSFAAFVLNVPLNYIFVYGKFGMPALGGAGCGLATMAVFWFSALALW
                     IYIAKENFFRPFGLTAKFGKPDWAVFKQIWKIGAPIGLSYFLEASAFSFIVFLIAPFG
                     EDYVAAQQVGISLSGILYMIPQSVGSAGTVRIGFSLGRREFSRARYISGVSLVLGWML
                     AVITVLSLVLFRSPLVSMYNNDPAVLSIAATVLLFAGLFQPADFTQCIASYALRGYKV
                     TKVPMFIHAAAFWGCGLLPGYLLAYRFDMGIYGFWTALIASLTIAAIALVWCLELCSR
                     EMVRSHKAV"
     gene            1749..2900
                     /locus_tag="WIC03_001103"
     CDS             1749..2900
                     /locus_tag="WIC03_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEZ3883613.1"
                     /translation="MQTWQLPEHIADVLPTNARQLESAREQLLALFRVHGYELVQPPL
                     MEYAHSLLTHIDAGLSLKTILVTDRLSGRQLGIRADITPQVARIDAHLLSANQGINRL
                     CYAGPVLHAQPDGLLNMREPLQAGAEMYGFADIRGDIELIDLMLKSMKIADMGKVLLS
                     LGHIGIFRALSDAAHLDAGQSATLLALMQDKDTGTVEAQVKAWKLDGMWAKAFSLLPR
                     LYGGREVLSDARGRLPDLSAVGGALDELQAVCDAFPDCEIHIDLSELRVDNYHTGLLY
                     AAYAADFHDAVARGGRYDGLGGYFGRARPATGFSFDLRSFIGRLPTSERQPAVLVDAE
                     DAEAAREAVEALREQGQCVVIDYGIGHNVSEELAGRLKKTDGVWQVVKR"
     gene            3004..4302
                     /locus_tag="WIC03_001104"
     CDS             3004..4302
                     /locus_tag="WIC03_001104"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEZ3883614.1"
                     /translation="MAKNVVVIGAQWGDEGKGKIVDWLAEEAGGVVRFQGGHNAGHTL
                     VVGGKKTILRLIPSGILHESLDCFIGSGVVVSPEALLGEIDELNAAGVKNVEGRLKIA
                     PTCPLILPYHIALDQAREASRGKGKIGTTGRGIGPAYEDKVARRAIRAADLLHPEKLR
                     EKLDAILAYYKVQLQHLHNAEPVKAEDVMAVIEKVALRIAPMITDVSRVLNEKNKNGE
                     KLLFEGAQGALLDIDYGTYPFVTSSNCLAGAASAGAGVGPQMLDYVLGIVKAYTTRVG
                     SGPFPTELFDEVGAGLAERGHEFGSVTGRARRCGWFDAAALKRSIQINGISGMCITKL
                     DVMDGVETINICVGYELPDGGKTDILPCGSDAVETCKPIYETMEGWKESTFGIKNYEE
                     LPDNAKAYLKRIEEVCGAPVAIVSTGPDREETIVLHHPFA"
     gene            4713..5150
                     /locus_tag="WIC03_001105"
     CDS             4713..5150
                     /locus_tag="WIC03_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ3883615.1"
                     /translation="MKISIFWLYNAFILERSIMPKSLSLQDVQLRFSSKFPDKTVLKF
                     TKNYEPVTIQCPLHGSVVYGNLQAAMKSSTGCPECTRNPEKPSPNAVAIRLEDTETGE
                     IYEFASTLAASKFIGCSNSTLAVRLSGRTPFDRLIRYRYRLVK"
     gene            5164..5574
                     /locus_tag="WIC03_001106"
     CDS             5164..5574
                     /locus_tag="WIC03_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ3883616.1"
                     /translation="MALTFTQAVSKLTSKFPHLNLVEFNGVRYPTVIVCPIHGRVTCS
                     TFKSMLDSKSGCPKCASYGVNSHKIPEDTIDKLSKNTVLEDTVTGETLTFPSRASAAR
                     SLGINPAAITDRIKGRVHTETLLAGRYKVHICTK"
     gene            5691..6083
                     /locus_tag="WIC03_001107"
     CDS             5691..6083
                     /locus_tag="WIC03_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ3883617.1"
                     /translation="MAKSFNQAASELTDIFPNISLTGFDGVNYPVTVNCPMHGNVRYS
                     TFNALIKSKYGCPECAKMSKTQTPPNVGKPLLILDTTTNETLTFPSVTAAGAALGVHF
                     QQINHRLKGRTSPDNLISNRYKVLGYDR"
     gene            6291..6683
                     /locus_tag="WIC03_001108"
     CDS             6291..6683
                     /locus_tag="WIC03_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ3883618.1"
                     /translation="MGKRMTFDTAKSRFQEKFPHLELLEFSGIYKPSSVRCPTHGVVQ
                     LLYYDTAIKSKYGCPECGKLKMKENTPPQNQKPVSILDTATGETLTFPSVQAAAKALN
                     TPYGSIRTKLDGRSNPDNLVCNRYKVLL"
     gene            6691..6878
                     /locus_tag="WIC03_001109"
                     /pseudo
     CDS             6691..6878
                     /locus_tag="WIC03_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229271.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6796..7356
                     /locus_tag="WIC03_001110"
     CDS             6796..7356
                     /locus_tag="WIC03_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883619.1"
                     /translation="MTANFAQTLVEIQDSLYRVVSTVQYGDDNLKRLTADKRKQYELN
                     FKISEGSTRVESDFKETLVRFGRDMLQDMPPKIRSATLVALTTLLVGGALGYGYLEYL
                     KQVASEGYQTERLYNAVDRLAESQERITSAILKGARGADFVQIGRRSYSREDISEANR
                     RAERVPYGAELVSDGNFTAVLSDIGD"
BASE COUNT         1939 a         1869 c         1926 g         1684 t
ORIGIN      
        1 tgttaatcca ctatattttt aatcggcgga gttttcagac agccttatgc gatctgacca
       61 tctccctact gcacaattcc aagcaccaca ccaaggcgat ggcggcgatg gtgagcgagg
      121 caatcaatgc cgtccagaag ccgtaaatgc ccatatcgaa acggtaggcg agcaggtagc
      181 ccggcagcag accgcagccc caaaaggcgg cggcgtggat gaacatcggc acctttgtaa
      241 ctttgtagcc gcgcaaggcg taggaggcga tacattgggt gaagtctgcc ggttggaaca
      301 agccggcgaa cagtaagacg gtggcggcga tgcttaaaac cgccggatca ttgttgtaca
      361 tacttaccag cggcgaacgg aataatacca aggaaagcac ggtaatcacg gcgagcatcc
      421 atcctaacac cagtgacacg cccgaaatat aacgcgcccg cgaaaattcg cgccgcccaa
      481 gcgaaaagcc gatgcgcacc gtccccgccg agccgacgct ttgcggaatc atatagagaa
      541 tccccgacaa actgatgccg acctgctgcg ccgccacata atcctcgccg aaaggcgcaa
      601 tcaaaaacac gataaacgaa aacgcgctgg cttccaaaaa ataagacagc ccgatgggtg
      661 cgccgatttt ccaaatctgt ttgaacaccg cccaatccgg tttgccgaat ttcgccgtca
      721 gtccgaatgg gcggaagaaa ttttccttgg cgatataaat ccacaatgcc agcgcgctga
      781 accaaaacac cgccatcgtc gccagtccgc agcctgcgcc gcccaaagcg ggcataccga
      841 atttgccgta aacgaaaata tagttcagcg gcacgttcaa cacaaacgcc gcaaagctga
      901 ccaacataat caggcgcggg cggttcaggc tggaggcgta ggcgtgcagt gcgcggtgta
      961 ccattgccgc cggcatcgcc aagctggtga acagcatata ctgcgccatt gtgccttcca
     1021 cataatcgct caaggtcagc cagttgcgga acggcgtaat cgccgcccac atcaagacca
     1081 tgccgaacac gcccaaaaac agcccgaacc aaatcccctg ccgtcccgtt tcgcccactt
     1141 cgtcggtttt acccgcgccg taaagctggg caatcatcgg gttcagcgcc gccataatgc
     1201 ccataaaggt aatataaacc gtggcaaacg cgctgctgcc caaagccacc gccgccaagt
     1261 cttccttgcc cgcaccgccc gccatcacgg tatcgacaaa accgatgccc acctgcgcga
     1321 cctgcgccaa cagcatgggc agggcaagag cggtcagcag gcggacttct ttcaggaaga
     1381 cggaaaagga aaagcggttg aggtcgagca gcataagtgt tcaatcaaca aaaatgccgt
     1441 ctgaagcaga aaacggcagc aggaagtgat gagaaataat ggtgcacatt atatcgtaaa
     1501 aaaatgctgt gccgtcagac ggcggataca gggtataaaa gtatattcag attgtgtgta
     1561 ttttatggta aagtttggtt ttaacgactt gacggcattg agccgtcgga caggggctgt
     1621 tcggattctg aatcggaagg aagcatcgcc gttttgacag cggcgtgatg cgttgcggca
     1681 aagatgccgt ctgattgcgg atcgggcagc cttttgtatt tacatgataa aatagaaggc
     1741 agattctcat gcagacatgg cagcttccgg aacatatcgc cgacgtactg cccacgaacg
     1801 cgcggcagct tgaaagcgcg agggagcagt tgttggcact gttccgcgta cacggttatg
     1861 aactggtaca gcctccgctg atggagtacg cacattccct gctgacgcat atcgatgcgg
     1921 ggctttccct gaaaaccatt ttggtaacgg acaggctcag cggcaggcag ttgggcatac
     1981 gcgccgacat cacgccgcag gtggcgcgta tcgatgccca tcttttatcc gccaaccaag
     2041 ggattaatcg gttgtgttat gccggtccgg tgttgcacgc gcagcccgac ggtctgctga
     2101 atatgcgcga acccttgcag gcaggggcgg aaatgtacgg ttttgccgac atccgtggcg
     2161 acatcgagct gatagacctg atgctgaaaa gcatgaaaat tgccgatatg ggcaaagtgc
     2221 tgctttcgct ggggcatatc ggcatatttc gcgccttgtc cgatgcggcg catttggatg
     2281 cggggcagtc tgcaacgctg cttgccttga tgcaggataa agataccggg acggtcgaag
     2341 cgcaggtcaa ggcttggaag ctggacggca tgtgggcaaa agcattctcg ctgctgccgc
     2401 gcctgtacgg cgggcgtgaa gtgttgtccg acgcgcgcgg acggttgccg gatttgtcgg
     2461 cggtcggcgg cgcgttagac gaattgcagg cggtgtgcga cgcattcccc gattgtgaaa
     2521 tccatatcga cttgtccgag ctgcgtgtcg acaattacca cacgggcttg ctgtatgccg
     2581 cctatgccgc cgatttccac gacgcggtcg cgcgcggcgg gcgttatgac ggattgggcg
     2641 gatatttcgg cagggcgcgc ccggcaacgg gattcagttt cgacttgcgc agctttatcg
     2701 ggcgtttgcc caccagcgaa cggcagcccg ccgtgttggt cgatgcggaa gatgccgaag
     2761 cggcgcgcga agcagtcgaa gccttgcgtg aacaagggca gtgtgtcgtg attgactacg
     2821 gtatcggaca caatgtttcg gaagagcttg caggccgtct gaaaaagacg gacggcgttt
     2881 ggcaggtcgt gaaacgctaa atacccgttc atggcggatg aaaggcaaat cgtggtgggg
     2941 cgcaaagccg caccggtttg gggatttccg caataatttt taatatcgat aggttatatg
     3001 gctatggcta aaaatgttgt agtaatcggc gcacagtggg gcgacgaggg taaaggtaaa
     3061 atcgttgact ggctggcgga agaagccggc ggcgtggtgc gcttccaagg cggccacaat
     3121 gcgggtcata ctttggtcgt cggcggcaaa aaaaccattt tgcgcctgat tccgagcggc
     3181 atcctgcatg aaagtctgga ctgcttcatc ggttcgggcg ttgtcgtctc ccccgaagcc
     3241 ctgttgggcg aaatcgacga gttaaacgct gcaggcgtga aaaacgtcga aggccgtctg
     3301 aaaatcgcgc cgacctgccc gctgatcctg ccttaccaca tcgcgctcga ccaagcccgc
     3361 gaagcatcgc gcggcaaagg caaaatcggc acgaccggcc gcggcatcgg ccctgcctac
     3421 gaagacaaag tggcacgccg cgccattcgc gccgccgatt tgctgcatcc tgaaaaactg
     3481 cgtgaaaaac tggatgccat ccttgcctat tacaaagtcc aactgcaaca tctgcacaat
     3541 gccgagccgg ttaaagcgga agacgtgatg gcggttatcg aaaaagtcgc gctgcgcatt
     3601 gcgccgatga ttaccgacgt gtcccgcgtg ttgaacgaga aaaacaaaaa cggcgaaaaa
     3661 ctgctgtttg aaggcgcgca aggtgcgttg ttggacatcg actacggcac ttatcccttc
     3721 gttacctcgt ccaactgtct ggcgggagcc gcttccgcag gcgcgggcgt aggccctcaa
     3781 atgctggatt atgttttggg catcgtcaaa gcctatacca cgcgcgtcgg ttcaggcccg
     3841 ttcccgaccg aattgttcga cgaagtaggc gcaggtttgg cggaacgcgg acacgaattc
     3901 ggctcggtaa ccggccgcgc acgccgctgc ggctggtttg atgccgccgc cctgaaacgc
     3961 tccatccaaa tcaacggcat ttccggtatg tgcattacca aactcgatgt aatggacggc
     4021 gttgaaacca tcaatatctg cgtcggctac gaattgcccg acggcggcaa aaccgatatc
     4081 ctgccttgcg gttcggatgc ggtggaaacc tgcaaaccga tttacgaaac aatggagggt
     4141 tggaaagaat cgactttcgg catcaaaaat tatgaggaac tgcccgataa tgccaaagca
     4201 tatttgaaac ggattgaaga agtctgcggc gcgccggtcg ccatcgtctc caccggcccc
     4261 gaccgagaag aaacgattgt gctgcatcat ccgttcgcat aaggttttgc agtaaaattg
     4321 ccgtctgaag ccctaatccg ccgttggtca taaatagtag ggtatcaaca tttcgggcta
     4381 caatggtacg tcagccaatg ccaagacgtg ccagcctgat ttgttgatgt gtgtactatt
     4441 gcataggcgg ttaagccatg caggagatga aagtgtatat ttcgcgcaaa gcccattagc
     4501 cgcaagcgag gggcggggtg cgcgagagag aggggggcag gtgctaagtc attggttatc
     4561 tccttactat ctatctaact acgggtgcat tatgcgccta tgcctgttgc ttgtcaaaca
     4621 ctatacagtt aaaatgtgta aagatttagt aaggacatat acccttttct ttacatttag
     4681 ctccatcgga taccagtgcg ttcagagaaa atttgaaaat ttctatattt tggttgtata
     4741 atgctttcat tttagaaagg tctataatgc caaaatcact ctcgctacaa gatgttcagt
     4801 taaggttttc cagtaaattt cctgataaga cggttttgaa gttcactaaa aactacgaac
     4861 cggtaacaat ccaatgccct ttgcatgggt cggttgttta cgggaatcta caagctgcca
     4921 tgaaatcctc aacaggatgt cctgaatgta ctcgaaatcc cgaaaagccc tctccaaacg
     4981 ccgttgccat taggttggag gacacagaga caggggaaat ctacgagttt gcaagtacac
     5041 tggctgccag taaatttatc ggttgctcca acagcacttt agctgtacgt ttaagcggac
     5101 gcactccgtt tgaccgattg attaggtatc gttataggtt ggtaaagtaa gttcacaacc
     5161 actatggcac ttacatttac tcaagcagtt tctaagctaa cctctaaatt tccacatttg
     5221 aatcttgtgg agttcaatgg cgttcgttac ccgacggtaa tcgtctgtcc tatacacggg
     5281 agggttactt gctctacatt caaaagtatg ttggactcta aaagtgggtg tccaaaatgt
     5341 gcatcttatg gtgtcaattc ccacaaaatt ccagaagata caatagataa attatctaaa
     5401 aatacagtat tggaggatac tgtaactggc gaaacactta cattcccctc aagagcatct
     5461 gctgcaaggt cattgggtat aaacccagca gctatcactg accgtataaa aggtcgggtt
     5521 cacacagaga ctttacttgc agggaggtat aaggttcaca tctgcactaa atgacgtata
     5581 cactttttaa cagtgtatac ccctcgccac tatcaggtgc ttctcgaaaa atttgacatt
     5641 tttataaatt tgctattaaa tccatttgac aatcaatttt ggagtctcaa atggccaaat
     5701 ctttcaacca agcagcttct gaacttactg atatattccc taatatctct ctaaccggct
     5761 ttgacggtgt gaattaccca gtaaccgtta attgtcctat gcacgggaac gttcgttatt
     5821 cgacatttaa tgcccttata aagtcaaagt acgggtgtcc tgagtgtgct aagatgtcaa
     5881 aaacccaaac acctccaaat gtagggaagc ccctcctcat cctcgacaca acgaccaacg
     5941 aaacactcac gttcccaagc gttactgccg caggtgctgc tttaggtgta catttccaac
     6001 aaatcaatca caggctcaag ggtcgaacgt cgcccgacaa ccttatttca aacaggtaca
     6061 aagtgcttgg gtacgatagg taaggttcac aacaccctaa atcctacgaa ccaagctcga
     6121 cggacgttca aatcccgatt agagttaatt tatgtaaaga tttagtaaag acgtatacca
     6181 tttttcttta cattgtgctt gcgcaggatt ccaggtaggt ctcaaaaaat ttgaaaattt
     6241 ctatattttg gttgtgtaat ccatttgcac ataacctatg gagacaaatt atgggtaagc
     6301 gaatgacttt cgataccgcc aaatcacgct ttcaagagaa atttccacat ttagaattgt
     6361 tggagttcag tggcatttat aaaccttcca gtgttagatg tcctacgcac ggggttgtcc
     6421 aacttttgta ttacgacaca gctataaagt caaagtatgg gtgtccggag tgcgggaaac
     6481 ttaaaatgaa ggaaaatacg cctccccaaa accaaaaacc tgtctccatc ctcgacaccg
     6541 ccacaggcga aacactcacg ttccccagcg tacaagctgc cgccaaagcc ctaaacaccc
     6601 cctacggctc tatacgaacc aagctcgacg gacgttcaaa tcccgacaac cttgtctgta
     6661 acaggtataa ggttctgcta taatcaacct atggaaaata ttgaagaata cgcactgctg
     6721 tctcccgaag ccctgctgga acgcctggat accgttttga gtatcagaat cggcggcaag
     6781 ggttgggaat ccagtatgac cgccaacttt gcacagacgc tggtcgaaat acaggacagt
     6841 ctgtacaggg ttgtgtcaac cgtccaatac ggggatgaca acctcaagcg gttgacagcg
     6901 gacaaacgga agcagtatga gttgaacttc aagatttccg agggttctac gcgtgtagag
     6961 tccgacttta aagagacttt ggttcggttc ggtagagata tgcttcaaga tatgccccct
     7021 aaaatccgtt cggcaacgct ggtagcgttg acgaccctgc ttgtcggagg ggcgttgggt
     7081 tacggttatt tggaatacct gaagcaggtt gcttcggaag ggtatcagac cgagcgtctg
     7141 tataatgccg tcgaccgtct tgcagaatcc caagaacgga taacgtccgc catcctgaag
     7201 ggtgctagag gtgccgattt cgtgcaaatc ggcagacgtt cctacagtag ggaggatata
     7261 tcggaggcaa atagacgtgc agagcgtgtc ccgtatggcg cagagttggt ttcagacggc
     7321 aattttaccg ctgttttatc tgatataggg gattaacaaa aatcaggaca aggcgacgaa
     7381 gccgcagaca gtataaatag tacggaaccg attcactt
//
LOCUS       DAWHSK010000085         7348 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.085, whole genome shotgun sequence.
ACCESSION   DAWHSK010000085 DAWHSK010000000
VERSION     DAWHSK010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7348
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.085"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(144..234)
                     /locus_tag="WIC03_001111"
     tRNA            complement(144..234)
                     /locus_tag="WIC03_001111"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(198..200),aa:Ser,seq:gga)
     gene            complement(273..359)
                     /locus_tag="WIC03_001112"
     tRNA            complement(273..359)
                     /locus_tag="WIC03_001112"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(323..325),aa:Leu,seq:cag)
     gene            complement(395..481)
                     /locus_tag="WIC03_001113"
     tRNA            complement(395..481)
                     /locus_tag="WIC03_001113"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Leu,seq:cag)
     gene            570..2945
                     /gene="rnr"
                     /locus_tag="WIC03_001114"
     CDS             570..2945
                     /gene="rnr"
                     /locus_tag="WIC03_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEZ3883620.1"
                     /translation="MNKNIKSLNLREKDPFLSREKQRYEHPLPSREWIIELLERKGVP
                     SKIEALVRELSIKEEEYEFFERRLKAMARDGQVLINRRGAVCAADKLDLVKCRVEAHK
                     DGFGFAVPLTPAKDGDFVLYERQMRGIMHGDIVTVRPAGMDRRGRREGTVLDIVERAQ
                     SKVVGRFYMDRGVAILEPEDKRLNQSIVLEPDGVARFKPESGQVIVGEIEVYPEQNRP
                     AVAKIIEVLGDYADSGMEIEIAVRKHHLPHQFSEACAKAAKKIPDHVRKSDLKGRVDL
                     RDLPLVTIDGETARDFDDAVFAEKIGRNYRLVVAIADVSHYVRPDDAIDTDAQERSTS
                     VYFPRRVIPMLPENLSNGICSLNPNVERLCMVCDMVITYAGNIKEYRFYPAVMRSHAR
                     LTYNQVWKWLSDGIGHPFKAQIDTLYKLFKILQKKRFERGAVEFDSIETQMLFDDNGK
                     IEKIVPVVRNDAHKLIEECMLAANVCAADFLLKNKHTALFRNHLGPTPEKLAALREQL
                     GLLGLQLGGGDNPSPKDYAALAGQFKGRPDAELLQVMMLRSMQQAVYEPHCDGHFGLA
                     YEAYAHFTSPIRRYPDLTVHRAIKAVLNQQTYTPKKSWQALGVHTSFCERRADDASRD
                     VENWLKTYYMRDKVGEVFEGKISGMTSFGIFVTLDGIHIDGLVHISDLGEDYFNFRPE
                     IMAIEGERSGIRFNMGDRVAVRVARADLDDGKIDFVLIAEGSGRGRKVKSSASAKPAG
                     TAGKGKPKTAAEKKTARGGKVRGRGASAAAESGKKAKKPVPIKVKKRKGKS"
     gene            complement(3284..4747)
                     /gene="guaB"
                     /locus_tag="WIC03_001115"
     CDS             complement(3284..4747)
                     /gene="guaB"
                     /locus_tag="WIC03_001115"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003767939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEZ3883621.1"
                     /translation="MRIVEKAYTFDDVLLVPAHSTVLPRDVKLQTKLTREITLNLPLL
                     SAAMDTVTEARLAISMAQEGGIGIIHKNMPPEMQARAVSKVKRHESGVVKDPVTVAPT
                     TLIREVLEMRAQRKRKMSGLPVVENGKVVGIVTNRDLRFENRVDLPVSAIMTPRERLV
                     TVPEGTSIDEARELMHTHKVERVLVLNEKDELKGLITVKDILKTTEFPNANKDSEGRL
                     RVGAAVGTGGDTEERVKALVEAGVDVIVVDTAHGHSQGVIDRVKWVKETYPHIQVIGG
                     NIATAKAALDLVAAGADAVKVGIGPGSICTTRIVAGVGVPQLTAIHNVAEALKGTGVP
                     LIADGGIRFSGDIAKALAAGAYSVMLGGMFAGTEEAPGEIELYQGRSYKSYRGMGSLG
                     AMSQGSADRYFQDKTDSTDKYVPEGIEGRVPYKGPIVNIIHQLTGGLRSSMGYLGCAN
                     IAEMHEKAEFVEITSAGMSESHVHDVQITKEAPNYHR"
     gene            complement(4948..5028)
                     /locus_tag="WIC03_001116"
                     /pseudo
     CDS             complement(4948..5028)
                     /locus_tag="WIC03_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798803.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(5074..5535)
                     /locus_tag="WIC03_001117"
     CDS             complement(5074..5535)
                     /locus_tag="WIC03_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883622.1"
                     /translation="MNIIMDSTRKFGILWEENSECNGFIYGKIQIIIGENIYPKICPY
                     GYFTLNAVFNSLKSSFEEKYYAGGNNGLDFGEQLFDIDKYNSLELCNIFSIDTTYMSG
                     GGNCEIDCLVLEMGYSGEEERLFYSFDNGKNFKEIRYKKGTVESVIFQLNL"
     gene            complement(5626..>7348)
                     /locus_tag="WIC03_001118"
     CDS             complement(5626..>7348)
                     /locus_tag="WIC03_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEZ3883623.1"
                     /translation="ELMRVFYRAIKTVKQLGGILTPMLQSRVSSTPLRVTLRIDDDYI
                     QVNNQIAARHTDIFFRRPEHIFKIVEIMQQRNDITALEPQTLRAWWGATRKINRSFYQ
                     NPENRRRFAGFFRNGNGLTQTLRFLNLYGVLGRYLPAWEKIIGLLQHDLFHIYPVDDH
                     ILTVVRNVRRLALDMHSHELPYASALMQSFEKQDILYLAAFFHDIAKGRGGDHAIQGI
                     ADARQFAADHFLTGEESDLLAWLVENHLLMSAVAQKEDIQDPDVLDAFCKRVQTHERL
                     SALYLLTISDIRGTNPKLWNAWRASLLESLFHAAGRYLTGNGGNPHTLFGRRRQEAAD
                     LLTRAAVPEKQQKKLWNALGSAYFARHQSREILWHAANLVHDFETPIVRSRILPQSDS
                     FQVMVFMPNGPRLFARLCRIFSRHGFDILAARAFITEHDYILDTFIVQIPSQHAPEDY
                     PDIQSALEAELNSFIHGHTVAEISSHSRRISRRSRYMPIAPSITITPEEDYPDWYSVE
                     ITAVNRPFLLADMAEVFFAHNVSLRYAKISTLDERAEDSFTVFSLDLKNPKIQSSLKQ
                     TLLEQLS"
BASE COUNT         1698 a         1766 c         2086 g         1798 t
ORIGIN      
        1 ataccacaaa acataccata aaaaaacggt aacggatagt tgtaagcggt tgatgacgat
       61 tatagacaat aacgggtttt cccaatgaaa ttattgtttg aataataaaa aatcccaaac
      121 cgtaaaagtt tgggatttgt atttggcaga gaggaaggga ttcgaaccct cgatacgcta
      181 ttcacgtata cacgctttcc aggcgtgcga cttaaaccac tcatccacct ctctaatggc
      241 ggaaattatc ccaatcggga taatttatta tttggtgccc gggagaagac tcgaacttcc
      301 acacccgtga ggataccagc acctgaagct ggcgcgtcta ccaattccgc cacccgggca
      361 atctaaatat tgaaataaag caaagcattt gatttggtgc ccgggagaag actcgaactt
      421 ccacacccgt gaggatacta gcacctgaag ctagcgcgtc taccaattcc gccacccggg
      481 ctttgcttta tttgctgttt tgcggtacag tgtcctgccg caaagaagtc gctattatat
      541 agttcataaa gagaatgtca acagtccaaa tgaataaaaa tattaaatct ttaaatttac
      601 gggaaaaaga cccgttttta agtcgtgaaa aacagcgtta tgaacatcct ttgcccagtc
      661 gggaatggat aatcgagctg cttgaacgta aaggcgtacc atccaagatt gaagctttgg
      721 tacgcgaatt gtcgattaag gaagaagagt acgaattttt cgaacgtcgt ctgaaggcga
      781 tggcgcggga cggtcaggtt ttaatcaacc gtcggggcgc ggtttgcgcg gcggacaaat
      841 tggatttggt caaatgccgt gtcgaggcgc acaaagacgg cttcggtttc gccgtgccgc
      901 tcacgcccgc caaagacggt gattttgttt tgtacgaacg ccagatgcgc ggcattatgc
      961 acggcgatat tgtcactgtt cgtcctgccg gcatggaccg taggggccgc cgcgaaggga
     1021 cggttctgga tattgtcgaa cgcgcgcaaa gcaaagttgt cggccgtttc tatatggata
     1081 ggggcgtggc gattttggag ccggaagaca agcgtctgaa ccaaagcatc gtattggaac
     1141 cggacggcgt ggcgcgtttc aaacctgaat ccggtcaggt catcgtcggc gaaattgagg
     1201 tttatcctga gcaaaaccgg ccggcagtgg caaaaatcat cgaagttttg ggcgattatg
     1261 ccgacagcgg catggagatt gaaattgccg tgcgcaagca tcatttgccg caccaattca
     1321 gtgaagcgtg tgccaaagcc gcgaaaaaaa ttcccgacca tgtacgcaaa agcgatttga
     1381 aaggccgcgt cgatttgcgc gatttgcctt tggtaacgat agacggcgaa acggcgcgcg
     1441 attttgacga tgccgtgttt gccgaaaaaa tcggacgcaa ttaccgtctg gtcgtggcga
     1501 ttgccgatgt cagccattat gtccgccccg atgacgctat cgacacggac gctcaggaac
     1561 gcagcaccag tgtttacttc ccgcgccgcg tgattcccat gttgccggaa aacctgtcca
     1621 acggcatctg ctcgctcaat cctaatgtcg agcgtttgtg tatggtgtgc gatatggtca
     1681 tcacttacgc gggcaatatt aaagaatacc gcttctaccc cgccgtgatg cgctctcatg
     1741 cccgcctgac ctacaaccaa gtttggaaat ggctttcaga cggcatcggg catccgttca
     1801 aagcacaaat cgacacgctt tacaaactct tcaaaatcct tcagaaaaag cgtttcgaac
     1861 gcggggcggt ggagtttgac agcatcgaaa cccaaatgct tttcgacgac aacggtaaaa
     1921 ttgaaaaaat cgtccccgtt gtccgcaacg atgcccacaa gctgattgaa gaatgtatgt
     1981 tggcggcaaa cgtttgcgca gcggattttc tgttgaaaaa caagcatacc gcattgttcc
     2041 gcaaccattt ggggcccacg cccgaaaaac tcgccgcctt gcgcgagcag ctcggtctgt
     2101 tggggcttca acttggcggc ggcgacaacc cgtcgccgaa agactatgcc gcgcttgccg
     2161 gacagttcaa aggcaggccg gatgccgaat tgctgcaagt catgatgttg cgctccatgc
     2221 aacaggcggt ttacgaaccg cattgcgacg gacactttgg tcttgcctac gaagcatacg
     2281 cccacttcac ctcgcccatc cgccgctatc ccgacctgac cgtacaccgc gccatcaaag
     2341 ccgtgttgaa tcagcaaacc tacacgccaa aaaaaagctg gcaggctttg ggcgtgcata
     2401 cctcgttctg tgagcgccgt gccgacgacg ccagccgcga cgtggaaaac tggctgaaaa
     2461 cctattatat gcgcgataag gtcggcgaag tattcgaagg taaaatctcc ggcatgacca
     2521 gttttggtat ctttgtaaca ctggacggca tccacattga cggcttggtg catatcagcg
     2581 atttgggcga agactatttc aacttccgcc ccgaaatcat ggcaatcgaa ggcgaacgca
     2641 gcggcatccg tttcaacatg ggggacaggg ttgccgtccg ggtcgcccgt gccgatttgg
     2701 atgatggaaa aatcgatttt gtcctgattg ccgaggggag cggcaggggg cggaaagtta
     2761 aatcatccgc gtctgccaaa ccggcaggga cggcggggaa agggaaaccg aaaaccgccg
     2821 ccgagaaaaa aacagcccga ggcggcaaag taaggggaag gggcgcgtct gccgccgcag
     2881 aatcggggaa aaaggcaaag aaaccggttc cgattaaggt aaaaaaacgg aaaggcaaat
     2941 cataatgctg acggggtggc ttggggaggg cggggcatac tttcccgaag ccgccgcccg
     3001 attttttgat atggcggccg gcgcatcatt tcaggccaat cgaaacgccc gtattgaaag
     3061 attgcgttta tttccaccgc cgttttaaag gccggcggta ttcggcagac ggggcgcaaa
     3121 cggcgttcag acggcatttt cattctttcg gcgtgtccgt ccgaatggct ttgcccgtcc
     3181 gcgcaatcag cccgtgcggc ttgccttgaa cggacaaaaa atgccgtctg aaacccgaaa
     3241 atcaggtttc agacggcatt ttgccttgaa aaggctgttc aaatcagcgg tggtagttcg
     3301 gtgcttcttt agtgatttga acgtcgtgaa cgtgcgattc gctcatacct gcggaagtga
     3361 tttccacaaa ttcggctttt tcgtgcattt cggcaatatt ggcgcaaccc aagtaaccca
     3421 tactggaacg cagaccgccg gtcagctggt ggatgatgtt cacaatcggg cctttgtaag
     3481 gaacgcggcc ttcgatgcct tcggggacgt atttgtcggt gctgtcggtt ttgtcttgga
     3541 agtagcggtc ggcagaacct tggctcatcg cgcccaatga acccataccg cgataggatt
     3601 tgtacgagcg gccttgatag agttcgattt cgcccggcgc ttcttccgtg ccggcaaaca
     3661 taccgccgag catgacgctg tacgcgcctg cggcgagggc tttggcgatg tcgccggaga
     3721 agcggatgcc gccgtcggca atcaggggaa cgcctgtgcc tttgagggct tcggcaacgt
     3781 tgtggatggc ggtcagttgc ggcacgccca cgcctgccac gatacgggtg gtgcaaatcg
     3841 atcccgggcc gataccgact ttgacggcat ccgcaccggc ggcgaccaaa tccaatgcgg
     3901 ctttggcagt ggcgatgttg ccgccgatga cttggatgtg cggataggtt tctttcaccc
     3961 atttcacgcg gtcaatcacg ccttggctgt gcccgtgggc agtatcgacg acaatcacgt
     4021 ccacgccggc ttcgaccaag gctttgacgc gctcttcggt atcgccgccg gtgccgactg
     4081 ccgcaccgac gcgcagacgg ccttcggagt ctttgttggc gttgggaaac tcggtggttt
     4141 ttaaaatatc tttaacggta atcagacctt tgagttcgtc tttttcgttc agaaccaaaa
     4201 cgcgctcgac tttgtgcgtg tgcatcagtt cgcgcgcttc gtctatgctt gtgccttcgg
     4261 ggacggtaac caaacgttcg cgcggggtca taatggcgga aacgggcaaa tcgacgcggt
     4321 tttcaaaacg caggtcgcgg ttggttacga tgccgacgac tttgccgttt tcaacgacgg
     4381 gcaggccgga cattttgcgt ttgcgctgcg cacgcatttc caagacttcg cggatgagcg
     4441 tcgtcggtgc aacggttacg gggtctttga ccacgccgct ttcgtggcgt ttcactttgg
     4501 aaacggcgcg cgcctgcatt tcgggcggca tgtttttatg gatgatgccg atgccgcctt
     4561 cctgtgccat cgaaatggcg aggcgcgcct cggtaacagt gtccatcgca gcggaaagca
     4621 gggggaggtt gagtgtgatt tcgcgggtga gcttggtttg aagtttaacg tctcgcggca
     4681 gcacggtcga atgtgcggga accaacaaaa catcgtcgaa agtataggct ttttctacga
     4741 tacgcataat gctcggtctt tcagtttgtg caagatgcac ggcattatag cacgttaccg
     4801 gcggcttgac agtttatcag gtttaatttt ggtccccttt gaatagcccg gttttccttt
     4861 gccgaccact gttgctcccg ttctttcaat ttcaggaaaa gcttttttct aatttttggt
     4921 aagtggctca gttattgaag ccctatatcg ggcggtaact tccagcacaa aaaaacggag
     4981 tagttcttta tttatttttt cctttaattt tcagtatatt atcttaatat ttcgagggta
     5041 acatatctgc taatctagtt acagccccat atattataga ttcaattgaa aaataacaga
     5101 ttcaactgta cctttcttat acctgatttc tttaaagttt ttcccattgt caaaactata
     5161 aaaaagtctc tcttcctcac cagaatatcc catttctaat actaaacaat ctatttcaca
     5221 attaccccct ccactcatat atgtagtatc aatagaaaat atattgcata attccagaga
     5281 attatattta tctatatcaa atagttgctc tccaaaatct aaaccattgt ttccacctgc
     5341 ataatacttc tcttcaaagg atgacttcaa actattaaaa actgcattta gcgtaaaata
     5401 tccatatggg catatcttag gatatatatt ctctcctatt attatctgaa tttttccata
     5461 aataaaacca ttacattcag agttttcttc ccataaaatc ccaaattttc ttgttgagtc
     5521 cataataata ttcatataaa tccttatatt aataaattat ttacaatatc ccccgctttc
     5581 agacggcata cggcgtggcg gcggaatgcc gtctgaaggc gggcgttatg ataattgttc
     5641 cagcagcgtc tgcttcaatg aggactggat ttttgggttt ttcaggtcta ggctaaaaac
     5701 ggtaaaactg tcttcggcgc gttcatccag tgtggagatt ttggcatagc gcaggctgac
     5761 gttgtgggcg aaaaagactt ccgccatatc ggcgagcagg aaggggcggt tgacggcggt
     5821 gatttcgacg gagtaccaat cggggtagtc ttcttcgggg gtgatggtga tgcttggcgc
     5881 gatcggcata tagcggctgc ggcggctgat gcggcggcta tggctggaga tttcggcaac
     5941 ggtgtgtccg tggataaagc tgttgagttc ggcttcgagc gcgctttgaa tgtcggggta
     6001 gtcttcggga gcgtgctgcg aggggatttg cacgatgaag gtgtcgagga tgtagtcgtg
     6061 ttcggtgatg aaggcgcggg cggcgagaat gtcgaagccg tggcggctga agatgcggca
     6121 gaggcgggcg aacaggcgcg ggccgttggg cataaagacc atcacttgga agctgtcgct
     6181 ttggggcagg atgcggctgc ggacgatggg ggtttcaaaa tcgtgtacga ggttggcggc
     6241 gtgccacagg atttcgcggg actggtggcg ggcgaagtag gcggaaccga gcgcgttcca
     6301 tagttttttc tgctgttttt cggggacggc ggcgcgggtg agtaagtcgg cggcttcctg
     6361 ccggcggcgg ccgaagaggg tgtgcgggtt gccgccgttg cctgtaaggt agcgtccggc
     6421 ggcatggaag aggctttcca gcaggctggc gcgccatgcg ttccacagct tgggattggt
     6481 accgcgtatg tcggaaatgg tcagaaggta gagcgcgctg aggcgttcgt gggtttggac
     6541 gcgtttgcag aaagcatcga gtacgtcggg gtcttggatg tcttcttttt gggcgacggc
     6601 agacatcagc aggtggtttt cgaccagcca agcgagcagg tcgctttctt ctccggtcag
     6661 gaagtggtca gcggcaaatt ggcgcgcgtc tgcgatgcct tgtatggcat ggtcgccgcc
     6721 gcgtcctttg gcgatgtcat ggaaaaaggc ggcaaggtag aggatgtctt gtttttcaaa
     6781 ggactgcatc agtgcagagg cgtagggcag ctcgtggctg tgcatatcca aggcaaggcg
     6841 gcggacgttg cggacgacgg tgaggatgtg gtcgtccacg ggatagatgt ggaacaggtc
     6901 gtgttggagc aggccgatga ttttttccca cgcgggcagg tagcggccca acacgccgta
     6961 gaggttgaga aagcgcaggg tctgggtcag cccgttgccg ttgcggaaaa aaccggcgaa
     7021 gcggcggcgg ttttcgggat tttggtagaa gctgcggttg attttgcgcg tcgcgcccca
     7081 ccaggcgcgc agggtttgcg gttcgagcgc ggtaatgtcg ttgcgctgct gcatgatttc
     7141 gacgattttg aaaatgtgtt cgggccgtct gaaaaaaata tcggtgtgcc gcgcggcgat
     7201 ttggttgttg acttggatgt agtcgtcgtc aatccgcagg gtaacgcgca acggggtgga
     7261 ggaaacgcgg ctttgcagca taggcgtgag gatgccgccc agttgtttga cggttttaat
     7321 cgcgcggtaa aacacgcgca tcagttct
//
LOCUS       DAWHSK010000086         7286 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.086, whole genome shotgun sequence.
ACCESSION   DAWHSK010000086 DAWHSK010000000
VERSION     DAWHSK010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7286
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.086"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(68..814)
                     /locus_tag="WIC03_001119"
     CDS             complement(68..814)
                     /locus_tag="WIC03_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEZ3883624.1"
                     /translation="MDFRFDIIYEYRWMFLYGALTTLGLTVVATAGGSVLGLLLALAR
                     LIHLEKAGAPMRVLAWALRKVSLLYVTLFRGTPLFVQIVIWAYVWFPFFVHPSDGILV
                     SGEAAIALRRGYGPLIAGSLALIANSGAYICEIFRAGIQSIDKGQMEAARSLGLTYPQ
                     AMRYVILPQALRRMLPPLASEFITLLKDSSLLSVIAVAELAYVQNTITGRYSVYEEPL
                     YTVALIYLLMTTFLGWIFLRLEKRYNPQHR"
     gene            complement(993..1664)
                     /locus_tag="WIC03_001120"
     CDS             complement(993..1664)
                     /locus_tag="WIC03_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEZ3883625.1"
                     /translation="MQIKKIMKWLPVALSLLGALGYTGYGSEAVRTAVAVLDVLGAAG
                     DAGSDAPARRRASAKSGHRYTGTVSKVYDGDTLHVIDGDGAKHKIRMAYIDAPEMKQA
                     YGTRSRDNLRAAVEGRKVSVRVFDTDRYQREVAQVSVGKTDLNLMQVQDGAAWHYKSY
                     AKEQQDKADFADYADAQIQAERERKGLWKAKNPQAPWAYRRAGRSGGGNKDWMDAVGE
                     WLGIW"
     gene            complement(1733..2404)
                     /gene="rpiA"
                     /locus_tag="WIC03_001121"
     CDS             complement(1733..2404)
                     /gene="rpiA"
                     /locus_tag="WIC03_001121"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEZ3883626.1"
                     /translation="MTTQDELKRIAAEKAVEFVPENEYIGIGTGSTINFFIEALGKSG
                     KKIKGAVSTSKKSSELLAQYDIPVVSLNEVSGLAVYIDGADEVNHALQMIKGGGGAHL
                     NEKIVASASEKFVCIADESKYVSRLGKFPLPVEVVESARSLVSRKLLAMGGQPELRIG
                     YTTFYGNQIVDVHGLNIDQPLTMEDEINKITGVLENGIFARDAADVLILGTEEGAKVI
                     YPCQG"
     gene            complement(2481..2963)
                     /locus_tag="WIC03_001122"
     CDS             complement(2481..2963)
                     /locus_tag="WIC03_001122"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEZ3883627.1"
                     /translation="MTNIRIGQGYDVHQLTEGRKLILGGVEIPFEKGLLGHSDADALL
                     HAVTDALLGAAGLGDIGSHFPDTAAEFKDADSRVLLRAAYQSVQAQGWQAVNVDTTVI
                     AQKPKLAPHIPQMRANIAADLGIDISCVNIKGKTNEKLGYLGRMEGIEAQAAVLLVRI
                     "
     gene            complement(2995..3684)
                     /locus_tag="WIC03_001123"
     CDS             complement(2995..3684)
                     /locus_tag="WIC03_001123"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEZ3883628.1"
                     /translation="MKRKNIALIPAAGIGARFGADKPKQYVEIGSKTVLEHTIGIFER
                     HEAVDLTVVVVSPEDTFADKVQTAFPQVRVWKNGGQTRAETVRNGVAKLLEIGLASAD
                     DNILVHDAARCCLPSEALTRLIEQAGNAAEGGILAISIADTLKRAEGGQISATVERTS
                     LWQAQTPQLFRAGLLHRALAAENLDGITDEASAVEKLGIRPLLVQGDARNLKLTQPQD
                     AYIVRLLLNAV"
     gene            complement(3681..4415)
                     /gene="dnaQ"
                     /locus_tag="WIC03_001124"
     CDS             complement(3681..4415)
                     /gene="dnaQ"
                     /locus_tag="WIC03_001124"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HEZ3883629.1"
                     /translation="MTTRQIILDTETTGLYADGGDRLVEFAGLEMVNRQMTDKNLHLY
                     VHPERDMPEEAARVHGLTIQVLEGKNAPPFAEVGRQIADFLRGAELIIHNAKFDVGFL
                     NMEFRRMGLPTVEELGCTVTDTLAMAREMFPGQKASLDALCNRFSVDRSKRVLHGALI
                     DCELLGEVYLAMTRRQFDLMGAAAEEKMETKPVVHTETKRSGKLKVIRADENELAAHG
                     QYLDGLGEACIWRKEAVPSENGGTDA"
     gene            complement(4571..5881)
                     /locus_tag="WIC03_001125"
     CDS             complement(4571..5881)
                     /locus_tag="WIC03_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ3883630.1"
                     /translation="MYAKKGGLGLVKSRRFAPLFATQFLGAFNDNVFKTALFVMIGFY
                     GLGQNGFLPAGQMLNLGALLFILPYFLFSSLSGQLGNKFDKAVLARWVKVLEMIIMAV
                     AAYGFYIRSAPLLLACLFCMGAQSTLFGPLKYAILPDYLDDKELMMGNSLIESGTFVA
                     ILFGQILGTAVAGVPPYIVGILVLLVAVGGTVGSLFMPSVPAKAADTQIEWNIVRGTK
                     SLLRETVRHKPVFTAIIGISWFWFVGAVYTTQLPTFTQIHLGGNDNVFNLMLALFSIG
                     IAAGSVLCAKFSRERLMLAWVTVGALGLTVCGLVLVWLTHGHRFEGLNGIFWFLSQGW
                     AYPVMAVMTLIGFFGGFFSVPLYTWLQTASSETFRARAVAANNIVNGIFMVSAAVLSA
                     VLLFLFDSISLLYLIVALGNIPLSVFLIKRERRFLGAAAIRKKP"
     gene            complement(5871..6056)
                     /gene="ccoS"
                     /locus_tag="WIC03_001126"
     CDS             complement(5871..6056)
                     /gene="ccoS"
                     /locus_tag="WIC03_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HEZ3883631.1"
                     /translation="MESMFILVPISIILAFVIGWFFWWSGKNGQFDDLEGPAHRILMD
                     DDSTSKLIEKERENDVR"
     gene            complement(6056..6355)
                     /locus_tag="WIC03_001127"
     CDS             complement(6056..6355)
                     /locus_tag="WIC03_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883632.1"
                     /translation="MHYFSIHDQSGRHIGFFILLADDESEARPQSGRFAVKLEGGMEN
                     HVLSPFGQTEIPQYWRVVKDRIELFFDEAPVGTLRNEYLTVSGQTFVLNDLTGNM"
     gene            6706..>7286
                     /locus_tag="WIC03_001128"
     CDS             6706..>7286
                     /locus_tag="WIC03_001128"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222237.1"
                     /note="AckA utilizes acetate and can acetylate CheY which
                     increases signal strength during flagellar rotation;
                     utilizes magnesium and ATP; also involved in conversion of
                     acetate to aceyl-CoA; also known to act on propionate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEZ3883633.1"
                     /translation="MSQKLILVLNCGSSSLKGAVLDNGSGEVLLSCLAEKLNLPDAYI
                     TFKVNGEKHKVDLSAHPDHTGAVEALMEELKAHGLDSRIGAIGHRVVSGGELYSESIL
                     VDDEVIAGIEKCIPLAPLHNPAHLLGLRAAQSIFKGLPNVVVFDTSFHQTMPEVAYKY
                     AVPQELYEKYGLRRYGAHGTSYRFVADETARFLG"
BASE COUNT         1719 a         2180 c         1771 g         1616 t
ORIGIN      
        1 ataaatttgc cacaaaaaag ctgcctcaaa tgaatacccg ggcagctttt tgttgatatg
       61 actccaatca gcggtgttgc ggattgtaac gtttttccaa acgcaggaat atccagccta
      121 agaaagtcgt catcaacaga taaatcaggg cgacggtgta aagcggttct tcataaaccg
      181 aataccggcc cgtaatcgta ttctgaacat acgccaactc cgccacagca atgaccgaca
      241 gcagcgagct gtctttcaag agcgtgatga actcgctcgc caaaggcggc agcatgcggc
      301 gcaatgcctg cggcagaatc acatagcgca tcgcctgcgg ataggtcagc cccaaagaac
      361 gcgccgcctc catctgtcct ttgtctatag actggatgcc cgcgcggaaa atctcacaga
      421 tatacgcccc cgagttggcg atcagtgcca aagaaccggc aatcagcggc ccgtatccgc
      481 gacgcagcgc gattgccgcc tcgccgctga ccaaaatgcc gtctgaagga tggacgaaaa
      541 acggaaacca cacatacgcc caaatcacaa tctgcacaaa cagcggcgta ccccggaaca
      601 gcgtaacata cagcagcgaa actttacgca acgcccacgc cagcacgcgc atcggcgcac
      661 cggctttttc caagtgaatc aggcgcgcca acgccaacaa cagacccaat accgaaccgc
      721 ccgccgtcgc cacgaccgtc agccccaagg tcgtcagtgc gccgtaaagg aacatccagc
      781 ggtattcgta aataatgtca aaacgaaaat ccatataccg tccgtatcaa aaaccggcgg
      841 aactgccgcc gttgcaaaat aatccgccat tttaccgtaa aaaccgccgc ctgaactttt
      901 ttatcgcggc agacggcggt tgcgcgtctc cgcaaaaatg cagggcgcgc ggttttcaga
      961 cggcatttgc cgttcaaagc cgtgcgctgt ctttaccaaa tgcccaacca ttcgcccacg
     1021 gcatccatcc aatccttatt gcccccgccg ctcctgcctg cccggcggta cgcccacggc
     1081 gcttgcggat ttttagcttt ccacaatcct ttgcgttccc tttccgcctg aatttgagcg
     1141 tcggcataat cggcaaaatc cgccttatcc tgctgttctt tagcataact tttataatgc
     1201 cacgccgccc cgtcctgcac ctgcatcagg ttcaaatcgg ttttgccgac agaaacctgc
     1261 gccacttcgc gctggtagcg gtcggtgtcg aacacgcgca cgctgacttt cctgccttcc
     1321 accgccgcac gcaggttgtc gcgcgaacgc gtgccgtaag cctgtttcat ctccggcgcg
     1381 tcgatatacg ccatccggat tttgtgtttc gcgccgtcgc cgtcgataac gtgaagggtg
     1441 tcgccgtcat agactttgga caccgtgcct gtgtagcggt ggccggattt cgccgatgct
     1501 cggcggcggg cgggcgcgtc ggaacccgcg tcccctgccg cgccgagtac gtcgagtacg
     1561 gcaaccgccg tccgcaccgc ctcgctgccg taccccgtat aacccaacgc acccaaaagc
     1621 gacaaggcga cgggaagcca tttcatgatt tttttaatct gcatattttt caaatgccga
     1681 tgccgtctga acatatcgga atcggatttc agacggcatc ttaacgtcag gattaccctt
     1741 ggcagggata gatgactttc gcaccctctt ccgtccccaa aatcaacaca tcggcggcat
     1801 cgcgggcgaa tatgccgttt tcgagcacgc cggtgatttt gttgatttcg tcttccatcg
     1861 tcagcggctg atcgatattc aagccgtgga catcgacgat ttggttgccg taaaacgtgg
     1921 tgtagccgat acgcagttcg ggctgtccgc ccatagcgag cagtttgcgc gaaacaagag
     1981 agcgcgcgct ttcgacgact tccacaggca gagggaattt gcccaaacgt gaaacatatt
     2041 tgctttcatc cgcaatgcag acgaattttt cggatgcgct ggcgacgatt ttttcgttga
     2101 ggtgtgcgcc gccgccgcct ttaatcattt gcagggcgtg gttcacttca tccgcaccgt
     2161 caatatagac cgccaacccc gatacttcgt tcagggaaac gacgggaata tcatattggg
     2221 caagcagttc actggatttt ttggaagtag ataccgcgcc tttgattttt ttgccgctct
     2281 tacccaaggc ttcgatgaaa aagttgatgg tcgaaccggt accgatgccg atgtattcgt
     2341 tttcgggtac gaattcgact gctttttcgg cggcgatgcg cttgagttcg tcttgtgtcg
     2401 tcatattttt gtcctttggg aaaccgtatc aacaaacagc cgccatctta acattttttt
     2461 gcacgtcctg cccgccgcgt tcaaatcctt accagcaata ccgccgcctg cgcctctatg
     2521 ccttccatgc gcccgagata accgagtttt tcgttggttt tgcctttgat gttgacgcac
     2581 gaaatgtcta tgcccaaatc ggcggcgatg ttggcacgca tttgcggaat atgcggcgcg
     2641 agtttgggtt tctgtgcaat cacggtcgta tcgacattga ccgcctgcca accctgcgcc
     2701 tgaacgcttt gatacgccgc acgcaaaagg acgcggctgt ccgcatcttt gaactctgcg
     2761 gcggtgtcgg ggaaatggct gccgatatcg cccaaacctg ccgcaccgag cagcgcgtcg
     2821 gtaacagcgt gcagcagcgc atcggcatcg gagtgtccga gcagcccttt ttcaaatggg
     2881 atttcaactc cgccaagtat cagctttctg ccttcggtca gttggtggac atcgtagccc
     2941 tgtccgatac ggatgttcgt catcgtttgt gttcctgatg ttttgaattg aagttcagac
     3001 ggcattgagc agcagcctga cgatgtatgc gtcctgcggc tgcgtcagtt tcaaattgcg
     3061 cgcgtcgccc tgcaccagca aagggcggat gcccaatttt tccacggcgg acgcttcatc
     3121 ggtaatgccg tccaagtttt ccgcagccaa tgcgcggtgc agcagccccg cgcggaaaag
     3181 ctgcggcgtt tgtgcctgcc aaaggctcgt ccgctcgaca gtggcactga tttgtccgcc
     3241 ttctgcgcgt ttgagcgtat cggcaatgga aattgccaaa atcccgcctt cggcggcgtt
     3301 gcccgcctgt tctatcaacc gcgtcaaagc ttcagacggc aggcagcagc gcgcggcatc
     3361 gtgtaccaga atattgtcat ccgccgatgc caagccgatt tccaacagtt ttgccacacc
     3421 gtttcggacg gtttcggcgc gggtctgtcc gccgtttttc cacacccgaa cctgtggaaa
     3481 tgccgtctga accttatcgg caaacgtgtc ttcgggcgag acgacaacga cggtcaaatc
     3541 gacggcctca tgccgttcaa aaatcccaat cgtatgttct aaaacggttt tgcttccgat
     3601 ttcgacatat tgcttgggtt tgtccgcacc gaaacgcgcc ccgatgccgg cggcgggaat
     3661 cagcgcgata tttttgcgct tcatgcgtcc gtcccgccgt tttcagacgg cacggcttcc
     3721 ttgcgccaga tacaggcttc gcccaagccg tccaaatatt gcccgtgcgc cgccaactcg
     3781 ttttcgtccg ccctgatgac tttcagtttg ccgctgcgtt tggtttcggt atgcaccacg
     3841 ggtttggttt ccattttttc ctctgcggcc gcacccatca ggtcgaactg ccgccgcgtc
     3901 atagcaagat agacttcgcc caaaagttcg cagtcgatca atgcgccgtg caggacgcgc
     3961 ttgctgcggt cgacggaaaa acggttgcac aaggcatcca ggctggcttt ctgcccgggg
     4021 aacatttcgc gcgccatcgc cagggtatcg gtaacggtac agccgagttc ctcaacggtc
     4081 ggcaacccca tccggcggaa ctccatattg aggaagccca cgtcgaattt ggcattgtgg
     4141 ataatcagtt ccgcaccgcg caggaaatcg gcaatctgcc tgccgacctc tgcaaacggc
     4201 ggcgcgtttt tcccttccaa aacctgtatc gtcaagccgt ggacgcgtgc cgcctcttcg
     4261 ggcatatcgc gctcggggtg gacatagagg tgcaggtttt tgtcggtcat ttggcggttg
     4321 accatttcca aaccggcaaa ctcgaccaag cggtcgccgc cgtcggcata cagaccggtg
     4381 gtttcggtat cgaggatgat ttggcgtgtc gtcatatcgg tgtctttctt ctatcttcgt
     4441 aaattgctta ttttttaagc aatgtatttt tctgttttca tttcaatgca caaacccact
     4501 tattcacagt gtgttcacaa cattgggcag gcggattgtg tattttgggg acaatttttt
     4561 cagacggcat tcaaggtttt ttcctgattg ccgccgcgcc taaaaaccgc ctttcgcgct
     4621 taatcaaaaa taccgacaac ggaatattgc ccaaagcgac aatcagatac aacaaggaaa
     4681 tgctgtcaaa caaaaacagc aacaccgcgc tcaaaacggc agcggaaacc ataaaaatac
     4741 cgttaacgat attgttggcg gcaacggcgc gggcgcggaa agtctcgcta ctggcggttt
     4801 gcagccaggt atagagcgga acggagaaaa atccgccgaa aaagccgatc agcgtcatca
     4861 ccgccatcac gggatatgcc catccttgcg ataaaaacca aaaaatgccg ttcagccctt
     4921 caaaacggtg tccgtgcgtc agccacacca aaaccaggcc gcaaaccgtc aaacccaacg
     4981 caccaaccgt tacccaagcc aacatcaggc gttccctgct gaacttggca cacagtaccg
     5041 aaccggcggc aataccgatg gaaaacagag caagcatcag gttgaaaaca ttgtcgttgc
     5101 cgcccagatg gatttgggta aaggtcggca gttgcgtggt ataaaccgcg ccgacaaacc
     5161 aaaaccacga aataccgata atggcggtaa aaacgggctt gtgccgcacc gtttcacgca
     5221 gcagggattt tgtgccacgg acaatattcc actcaatttg tgtatcggca gccttggcgg
     5281 gtacggacgg cataaacagg ctgccgaccg tgcctccgac ggcgaccagc aaaaccagta
     5341 tcccgacaat ataaggcggt acacctgcca ccgccgttcc caaaatctga ccgaacagga
     5401 tggcgacaaa cgtacccgat tcaatcaggc tgttgcccat catcaactct ttgtcgtcga
     5461 gataatcggg caggatggcg tatttcagcg gcccgaacag cgtcgattgc gcgcccatgc
     5521 aaaacagaca cgccaaaagc agcggggcag accggatata aaacccgtat gccgccaccg
     5581 ccataatgat catttccagc accttgaccc aacgcgccaa aacggccttg tcgaatttgt
     5641 tacccaactg ccccgacagc gaggaaaaca ggaaatacgg caaaataaac agcaacgcgc
     5701 ccaagttcaa catctgtccg gcaggcagga agccgttttg ccccaaaccg taaaacccaa
     5761 tcatcacaaa cagcgcggtt ttgaacacat tgtcgttgaa cgcgccgaga aactgcgtag
     5821 cgaaaagagg tgcgaaacgg cggcttttaa ccagtcccaa accgcctttt ttagcgtaca
     5881 tcgttttccc tctctttttc aatcagttta cttgtcgaat catcatccat caggatgcgg
     5941 tgcgccggcc cttccaagtc gtcaaactgc ccgtttttgc ccgaccacca aaaaaaccag
     6001 ccgatgacaa acgccaaaat aatgctgatg ggcaccaata taaacatgct ttccatcaca
     6061 tattccctgt caaatcgttc aaaacaaaag tctgccccga cacggtcaga tattcgttac
     6121 gcaaagttcc gacgggagct tcgtcaaaaa acagctcgat acggtctttg accacgcgcc
     6181 aatattgggg gatttccgtc tgaccgaacg gcgacaggac atgattttcc attccgcctt
     6241 caagtttgac ggcaaaacgc ccgctttgcg gccgtgcttc cgattcgtcg tcggcaagca
     6301 ggatgaaaaa gcctatatgc cgtcccgatt ggtcatgaat actgaaataa tgcataaatt
     6361 tcccacccgc cttttttcag acgacaccaa ctaaaaacag ggcgaatgta ccagtttgga
     6421 cgggaagaat gcaaagaaat tctccctccc ccagccgaaa acaccggcaa accgcatatc
     6481 cccctttttt ccgtcaaaat gcccgacttc cgccattttc acgcaaacgc ccgattaagc
     6541 caagcaattg caaagatttt ttgctagaat agcctgcttc ttttatcaac cttttcagac
     6601 ggccccacta ctttcccgcc caggaaggca aaacggattc ggcacgaatc cggttagtat
     6661 ccgtgtccga ttccaatgcc gtctgaaact ttccggagta agaaaatgtc ccaaaaattg
     6721 atcttggttt tgaactgcgg cagctcgtcc ctcaaaggcg cggtcctgga taacggcagc
     6781 ggcgaagtcc tgctcagctg ccttgccgaa aaactcaacc tgcccgatgc ctacatcaca
     6841 ttcaaagtaa acggcgaaaa acacaaagtc gatctgtccg cacatcccga ccacaccggc
     6901 gcggtcgaag ccctgatgga agaactcaaa gcccacggcc tcgacagccg catcggcgcc
     6961 atcggccacc gcgtcgtcag cggcggcgaa ctgtacagcg aatccatcct cgttgacgac
     7021 gaagtcattg ccggcatcga aaaatgcatc ccgctcgccc ccctgcacaa ccccgcccac
     7081 ctcttgggcc tgcgtgccgc gcaaagcatt ttcaaaggcc tgcccaacgt cgtcgtattc
     7141 gatacctcct tccaccaaac catgcccgaa gtcgcctaca aatacgccgt tccgcaggag
     7201 ttgtatgaaa aatacggcct gcgccgttac ggcgcgcacg gtaccagcta ccgcttcgtc
     7261 gccgacgaaa ccgcgcgctt cctcgg
//
LOCUS       DAWHSK010000087         7272 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.087, whole genome shotgun sequence.
ACCESSION   DAWHSK010000087 DAWHSK010000000
VERSION     DAWHSK010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7272
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.087"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..174)
                     /locus_tag="WIC03_001129"
     CDS             complement(<1..174)
                     /locus_tag="WIC03_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883634.1"
                     /translation="MPSERLSDGIFPCVLNRAHETPDAFGGNVGCRAMFGGDQRTKYA
                     GKGLMPSEGSDGIG"
     gene            complement(192..749)
                     /locus_tag="WIC03_001130"
     CDS             complement(192..749)
                     /locus_tag="WIC03_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEZ3883635.1"
                     /translation="MDALKLLTNRRSSKKLKPPAPDAAELEQIFQAATQVPDHGNMRP
                     FRFTVIQGEVGLQRFRDVLKQTVAELNFGDDAMKKAEKVGNMAPMVIGVTFAPNQKVA
                     KPKPEWEQMLTAGCAAYALQLAATAQGFDNVWITGMWVNSPLLREAFGCADKDKIIGL
                     MMIGTPTEEAHKPKNTDLEAFVSHW"
     gene            complement(825..2459)
                     /locus_tag="WIC03_001131"
     CDS             complement(825..2459)
                     /locus_tag="WIC03_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipooligosaccharide phosphoethanolamine
                     transferase LptA"
                     /protein_id="HEZ3883636.1"
                     /translation="MIKPNLRPKLGSSALIAFLSLYSSLVLNYAFFAKVVELHPFNGT
                     GADIFLYTMPVVLFFLSNFVFHVIALPFVHKVLIPLILVISAAVSYQEIFFNIYFNKS
                     MLNNVLQTTAAESARLITPGYVLWIVCLGVLPALAYIAVKVKYRVWYKELLTRLVLAA
                     VSFLCALGIAMLQYQDYASFFRNNKSVTHLIVPSNFIGAGVSKYKDWKRSNIPYTQLD
                     MAVVQNRPVGSLRRFVVLVVGETTRAANWGLNGYSRQTTPLLAARGDEIVNFPQVRSC
                     GTSTAHSLPCMFSTFDRTDYDEIKAEHQDNLLDIVQRAGVEVTWLENDSGCKGVCGKV
                     PNTDVTSLNLPEYCRNGECLDNILLTKFDEVLNKNDKDAVLILHTIGSHGPTYYERYT
                     EAERKFTPTCDTNEINKCTRATLVNTYDNTVLYVDQFIDKVIRKLENRDDLESVVHYV
                     SDHGESLGENGMYLHAAPYAIAPSGQTHIPMVMWFSKAFRQHGGIDFQCLKQKAAENE
                     YSHDHYFSTVLGLMDISNSQTYRKEMDILAACRRPR"
     gene            complement(2861..3967)
                     /gene="serC"
                     /locus_tag="WIC03_001132"
     CDS             complement(2861..3967)
                     /gene="serC"
                     /locus_tag="WIC03_001132"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HEZ3883637.1"
                     /translation="MSLYPIYNFSAGPAVLPEAVLETARQEMLDYNGTGFPVMAMSHR
                     SEMFLSILHHAEQDLRQLLKVPDNYKILFLQGGATTQFNMAAMNLAHGFRTADAVVTG
                     NWSRIAYEQMSRLTDTEIRLAAHGGEQFDYLDLPPVETWDVAPDSAFVHFAVNETVNG
                     LQYREVPRLSEGMPPLVCDMSSEILSREFDVADYGLIYAGAQKNIGPAGVTVVIVRED
                     LLERCPNDIPDVFNYRSHLNREGMYNTPSTYAIYMSGLVFRWLQAQGGVKKIEAVNRL
                     KAQTLYETIDGSGGFYINRIRPNARSKMNVVFQTGDEELDRRFVLEAELQGLCLLKGY
                     KSVGGMRASIYNAMPLEGVRALADFMRDFQRRYG"
     gene            4332..4763
                     /gene="rimP"
                     /locus_tag="WIC03_001133"
     CDS             4332..4763
                     /gene="rimP"
                     /locus_tag="WIC03_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003685973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEZ3883638.1"
                     /translation="MDIQTILEKTLPGLGYELVDFELTAQGTLRVFIDKEGGITVEDC
                     ATVSNHLSRVFMVEDIDYKNLEISSPGLDRPLKKAADFVRFAGQNAKIKTRLPIDGQK
                     NFIGKIEGCENDTVTISFDGKTVQIELGNIDKARLRPEFKF"
     gene            4791..6293
                     /gene="nusA"
                     /locus_tag="WIC03_001134"
     CDS             4791..6293
                     /gene="nusA"
                     /locus_tag="WIC03_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEZ3883639.1"
                     /translation="MSREMLQLAEALASEKNVDAEVVFQALEFALSTAAKKKADREHM
                     DVRVQINRDTGEYQTFRRWLIVADEDYTYPDVEKTIEEIQEEIPGTAIQIGEYYEEQL
                     PNEGFGRQAAQTAKQIILQRIRDAEREQNLNEFLAVKEDIVSGTVKRVERHGIIVEVV
                     AGKLDALIPRDQMIPRENFRSGDRIRALFLRVEEIGNIGRKQVILSRTSGDFLVKLYA
                     NEVPEIADGMLEIRAVARDPGQRAKVAVKANDQRIDPQGTCIGVRGSRVNAVSNELSG
                     ERIDVVLWSPEPAQFVMSALSPAEVSRIVIDEDKHAVDVIVAEDQLALAIGRGGQNVR
                     LASDLTGWQLNIMTSAEADERNAAEDAAIRRLFMDYLNVDEETADVLVQEGFATLEEV
                     AYVPAAELLAIEGFDEEIVDMLRNRARDAILTMAIAAEEKLGEVSDDMRNLEGIDADM
                     LRSLAEAGITTRDDLAELAVDELIEITGVNEETAKAVILTAREHWFTEDK"
     gene            6305..>7272
                     /locus_tag="WIC03_001135"
     CDS             6305..>7272
                     /locus_tag="WIC03_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HEZ3883640.1"
                     /translation="MSNTTVEQFAAELKRPVEDLLKQLKEAGVSKNSGSDSLTLDDKQ
                     LLNAYLTKKNGSNGGTISIRRTKTEVSTVDGVKVETRKRGRTVNIPSAEELAAQVKAA
                     QTQAAPVRPEQTAEDAAKARAEAATRAEARAKAEAEAAKLKAAKAGNKAKPAAQKPTE
                     AKAETAPVAAETKPAEESKAGKAQADKMPSKKPAEPKEKAAKPKHERNGKGKDAKKPA
                     KPAAPAVPQPVVSAEEQAQRDEEARRAAALRAHQEALLKEKQERQARREAMKQQAEQQ
                     AKAAQEAKTGRQRPAKPAEKPQAAAPAVENKPVNPAKAKKEDRRNR"
BASE COUNT         1962 a         2100 c         1800 g         1410 t
ORIGIN      
        1 accgatgccg tctgaacctt cagacggcat gagacctttg cctgcgtact tggtacgctg
       61 gtcgcctccg aacatggcgc gacacccgac atttccgccg aacgcatcgg gcgtttcatg
      121 agcccggttt aaaacgcatg gaaaaatgcc gtctgaaagc ctttcagacg gcattttgtt
      181 tgagattccg tttaccaatg gctgacaaac gcttccaaat cggtattttt gggcttatgc
      241 gcttcctctg tcggcgtgcc gatcatcatc agcccgatga ttttatcctt atccgcacat
      301 ccgaaagcct cacgcaacaa ggggctgttg acccacattc ccgttatcca cacattatcg
      361 aaaccttgcg ccgttgccgc cagctgcaag gcatacgccg cacagcctgc cgtcagcatc
      421 tgctcccatt ccggcttcgg cttggcgact ttctggttgg gcgcaaacgt taccccgatg
      481 accatcggcg ccatattgcc cactttttca gcctttttca tcgcatcgtc gccgaaattc
      541 aattcggcaa ccgtttgctt caacacatcg cgaaaacgtt gcaatcctac ctcgccttga
      601 atcacggtaa aacggaaggg gcgcatattg ccgtgatcgg gaacttgggt tgccgcctga
      661 aatatttgtt ccaactccgc cgcatcgggg gcggggggct tcagcttttt ggaagatcgg
      721 cggttcgtca ataattttaa agcatccata tcgttatact ccggtcatcg gtcgggtgtt
      781 cggacacata tgccgtccga aggcttcaga cggcatatcc ggcatcagcg cggacggcgg
      841 caggctgcca atatatccat ttccttccga taggtttgac tgttggaaat gtccatcagc
      901 cccaataccg tgctgaaata gtggtcgtgc gaatattcgt tttccgccgc tttttgtttg
      961 aggcattgga aatctatgcc cccgtgttgg cggaaggctt tggaaaacca cataaccatc
     1021 gggatatgcg tctgcccaga aggcgcgatg gcgtaaggcg cggcgtgcag gtacatcccg
     1081 ttttcgccca aactttcgcc gtggtcggaa acataatgca ccacgctttc caaatcgtcg
     1141 cggttttcaa gtttgcggat aaccttgtcg ataaactggt ccacatacaa aaccgtattg
     1201 tcgtaagtgt tgaccagcgt ggcgcgggtg catttgttga tttcgttggt gtcgcaggtc
     1261 ggcgtgaatt tgcgttcggc ttcggtatag cgttcgtaat acgtcggccc gtggctgccg
     1321 atggtatgca ggattaaaac cgcgtcttta tcgtttttgt tgaggacttc gtcgaactta
     1381 gtcagcagga tattgtcgag acactcgccg ttgcggcagt attcgggcag gttgagcgag
     1441 gtaacgtcgg tattcggcac tttgccgcac acgcccttgc agccggaatc gttttccaac
     1501 caagtaactt ccacgccggc gcgctgcacg atgtccagca ggttgtcttg gtgttcggct
     1561 ttgatttcgt cataatccgt gcggtcgaag gttgagaaca tacacggcag ggagtgcgcg
     1621 gtcgatgtgc cgcagcttct gacctgcggg aaattgacaa tttcatcgcc gcgcgcggca
     1681 agcagcggcg tagtttggcg gctgtaaccg ttcaaacccc agttggcggc acgcgtggtc
     1741 tcgcccacga ccagcaccac gaaacggcgc aggctgccga ccggccggtt ttgcacgacc
     1801 gccatatcca attgcgtata aggaatattg gaacgcttcc aatctttgta tttcgacacg
     1861 cccgcgccga tgaaattaga cggcacaatc agatgggtta ctgatttatt gttgcggaaa
     1921 aacgaggcgt aatcctgata ttgcaacatt gcgatgccca acgcgcacaa aaaggaaacg
     1981 gcggcaagca caaggcgtgt caaaagctcc ttataccaaa cgcggtattt aaccttgacg
     2041 gcgatatacg ccagcgcggg caatacgccc aaacatacaa tccacagcac atagcccggc
     2101 gtaatcaggc gcgcgctttc ggcagccgta gtttgcaaga cattattcaa catcgacttg
     2161 ttgaaataga tattgaaaaa tatttcttgg taagacaccg ccgcactgat aaccaatatc
     2221 aacggaatca ataccttatg cacgaaaggc agggcaatga cgtgaaaaac gaaattactt
     2281 aaaaaaaaca gcaccaccgg catcgtatag aggaagatat ccgccccggt gccgttaaaa
     2341 ggatgaagct cgacaacttt ggcaaaaaag gcgtaattca ataccagcga ggaatacagg
     2401 gaaaggaagg caatcagcgc ggaagagccg agcttcggcc tcaggttcgg ttttatcatt
     2461 tggaatgtgt cggataaggg ttggaaaagg catccggcat ttggaatccg gattattgaa
     2521 aaagattctt aattataagg caacggagca aagcagggca agaaaacggc ggctgtgcgg
     2581 gggttccgcc tgccattcaa acgtccggca gacataaaaa catcgtaagc aagattcgaa
     2641 ccggtctgca accgcccctg ccagaaaaac gggcaaagca tttcatattg gaaaaacccg
     2701 accgcgccgc cgacgggaca gtccggcaca aacagcatca cgctcggatt gaaaaggacg
     2761 gatagccgcc ggcagcacaa tcttaccacc tccaaaacgc cgccggagaa cgccgaaaca
     2821 gccgatgccg tctgaagccg cttcagacaa catcgggaca tcaaccgtaa cgccgttgga
     2881 aatcgcgcat aaaatctgcc aaagcccgca cgccttcaag cggcatcgca ttataaatgc
     2941 tggcgcgcat cccaccgaca gacttatagc ccttcaacag gcacaagccc tgcaattcgg
     3001 cttccagcac aaaacggcgg tcaagctcct catcccccgt ttggaacacg acattcattt
     3061 tagaacgcgc attcggacgg atacggttga tatagaaacc gccgctgcca tctatcgtct
     3121 catacaaggt ttgcgccttc agccgattga ccgcttcaat ttttttcaca ccgccctgcg
     3181 cctgcagcca gcggaacacc agccccgaca tataaatcgc gtaagttgac ggcgtgttgt
     3241 acataccttc gcggttcaaa tgcgaacggt agttgaacac atcgggaata tcgttcggac
     3301 aacgctcgag caaatcctca cgcacaatca ccaccgtaac ccctgccggg ccgatgtttt
     3361 tctgtgcgcc tgcgtaaatc agtccgtagt cggcaacatc aaactcgcgc gacaaaatct
     3421 cgctggacat atcgcacacc agcggcggca tgccttctga aaggcgcggc acttcacggt
     3481 attgcagccc gttgaccgtt tcattgacgg caaaatggac aaacgccgaa tcgggtgcaa
     3541 catcccacgt ttccacaggc ggcaggtcga gatagtcgaa ctgctcgccg ccatgcgccg
     3601 ccaaacggat ttccgtatcg gtcaaacggc tcatctgttc ataagcgata cggctccagt
     3661 tgcccgttac caccgcgtcg gcagtgcgga aaccgtgtgc cagattcatg gctgccatat
     3721 taaattgggt tgttgctccg ccctgcagaa acaatatctt atagttgtca ggcactttca
     3781 aaagctgcct caaatcctgt tccgcatgat gcaggatgct caaaaacatt tccgaacggt
     3841 ggctcatcgc catcacggga aaacccgtac cgttgtagtc caacatttcc tgccgcgccg
     3901 tttccaacac ggcttcgggc aatacggcag ggccggcgga aaaattgtaa atcggataaa
     3961 gagacatgat gcagccttga ttctgaacaa taacccgccc gattttaggc ttgcagcagg
     4021 ctttgtgcaa ggatggaaaa tacctgtcct ccgccccgct tcatgccgcc cgaacacaga
     4081 aaacaacatc aatatattga tttacaaaca taaaaatcat gcacgcgaca aatagataca
     4141 tttgttttgt caacaatatt cacgatttcc cattacaaac ctcccttaca tccgcttttt
     4201 tccgtcccaa aaacacaaaa taaatcaaca ctttcatttc tccgcaaaag cagttataat
     4261 tacgccgatt tttcaacttt gacgaaaaat ggccacgggg ccattttttt attatgtaca
     4321 tagggagcag catggatatc caaaccatcc tcgaaaaaac cctgcccggc ctgggctacg
     4381 aactggtcga tttcgaactg accgcgcaag gaacattgcg cgtgttcatc gacaaagaag
     4441 gcggcattac cgtcgaagac tgcgcaaccg tcagcaacca tttgagccgc gtcttcatgg
     4501 ttgaagacat cgactacaaa aacctggaaa tttccagccc cggactcgac cgtcccttga
     4561 aaaaagccgc cgacttcgtg cgctttgccg gtcagaatgc caaaatcaaa acccgcctgc
     4621 cgatagacgg tcagaaaaac tttatcggta aaatcgaagg ctgcgaaaat gataccgtta
     4681 ccatatcctt cgacggcaaa accgtacaaa tcgaattggg caacatcgac aaagcccgtc
     4741 tgcgccccga attcaaattc taaaacacag caatattgga gatgttcaaa atgagtcgtg
     4801 aaatgttaca gctggcagaa gcactggcaa gcgaaaaaaa cgttgatgcg gaagtcgtct
     4861 tccaagcact ggaattcgcc ctgtctaccg ccgccaagaa aaaggcagac cgcgaacaca
     4921 tggacgtgcg cgtccaaatc aaccgcgaca ccggcgaata ccaaaccttc cgccgctggc
     4981 tgattgtcgc cgatgaagac tatacctatc ccgatgtcga aaaaaccatc gaggaaatcc
     5041 aagaggaaat tcccggcacc gccatccaaa tcggcgaata ctatgaagag cagctaccca
     5101 acgaaggctt cggccgccaa gccgcacaaa ccgccaaaca aatcatcctg caacgcatcc
     5161 gcgatgcgga gcgcgagcaa aatctgaacg agtttctcgc cgtcaaagaa gacatcgtgt
     5221 ccggtacggt caaacgcgtc gaacgccacg gcatcatcgt cgaagtcgtt gccggcaaac
     5281 tggacgcgct gattccgcgc gaccaaatga ttccgcgcga aaacttccgc agcggcgacc
     5341 gcatccgcgc cctcttcctg cgcgtcgaag aaatcggcaa catcggccgc aaacaagtca
     5401 tcctgagccg tacttccggc gatttcctcg tcaaactgta cgccaatgaa gtacctgaaa
     5461 ttgcagacgg catgcttgaa atccgcgctg tcgcccgcga cccgggacaa cgtgccaaag
     5521 tcgccgtcaa agccaacgac cagcgcatcg atccgcaagg cacctgtatc ggcgttcgcg
     5581 gttcgcgcgt caacgcggtc agcaacgaat tgtccggcga acgcatcgac gttgtccttt
     5641 ggtcgcccga gcctgcccaa ttcgtcatga gcgcgctctc acccgccgag gtcagccgca
     5701 tcgtcatcga cgaagacaaa cacgccgtcg atgtcatcgt tgccgaagac cagctcgcgc
     5761 tcgccatcgg acgcggcggt caaaacgtgc gccttgcttc cgacctgact ggctggcagc
     5821 tcaacatcat gacctctgca gaagcagacg agcgcaatgc ggcagaagat gccgccatcc
     5881 gccgcctgtt tatggattac ttgaacgtgg acgaagaaac cgccgacgta ctggttcagg
     5941 aaggttttgc aaccttggaa gaagtcgcct atgttcctgc cgccgaactg cttgccattg
     6001 aaggatttga cgaagaaatc gtcgatatgc tccgcaaccg cgcccgcgat gccatcctga
     6061 ccatggcgat tgccgccgaa gaaaaactgg gcgaagtatc cgacgatatg cgcaacctcg
     6121 aaggcataga tgccgatatg ctccgcagcc ttgccgaagc aggcattacc acccgcgacg
     6181 acttggcaga gcttgctgtg gacgaactga ttgaaatcac cggtgtaaac gaagaaaccg
     6241 caaaagccgt catcctgacc gcacgcgaac actggtttac cgaagacaaa taaagggggt
     6301 acagatgagt aacacaaccg tagaacaatt tgccgccgag ctgaaacgcc ccgtcgaaga
     6361 cctgttgaaa cagttgaaag aagccggcgt cagcaaaaac agcggcagcg attccctgac
     6421 gctggacgac aaacagcttc tgaacgccta cctgaccaag aaaaacggca gcaacggcgg
     6481 caccatcagc atccgccgca ccaaaaccga agtcagcacc gttgacggcg taaaagtcga
     6541 aacacgcaaa cgcggacgca ctgtcaacat tccttctgcc gaagaattgg cagcacaggt
     6601 aaaagccgcc caaacccaag ccgcacctgt ccggccggag cagacggcag aagacgcggc
     6661 aaaagcccga gccgaagctg ccacacgtgc agaagcccgt gccaaggcag aagcggaagc
     6721 ggcaaaactg aaagcggcaa aagcaggcaa caaagccaaa cctgccgcgc agaaacccac
     6781 cgaagcaaaa gccgaaaccg cacccgttgc ggcggaaacc aaacccgccg aagaaagcaa
     6841 agcgggaaaa gcccaagccg acaaaatgcc gtctaaaaaa cccgccgagc ccaaagaaaa
     6901 agccgccaag ccgaaacacg agcgaaacgg caaaggcaaa gatgccaaaa aaccggcgaa
     6961 acctgccgca cctgccgtgc cgcaacccgt ggtcagcgcg gaagaacagg cgcaacgcga
     7021 tgaagaagca cgccgtgccg ccgcacttcg cgcccaccag gaagccctgt tgaaagagaa
     7081 acaggaacgc caggcacgcc gcgaagccat gaaacaacag gcagaacaac aggcaaaagc
     7141 cgcacaggaa gccaaaaccg gcagacagcg tcccgccaaa cctgccgaga aaccgcaggc
     7201 agccgcgccg gccgtcgaaa acaaacctgt caatccggca aaagcgaaaa aagaagaccg
     7261 ccgcaaccgc ga
//
LOCUS       DAWHSK010000088         7217 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.088, whole genome shotgun sequence.
ACCESSION   DAWHSK010000088 DAWHSK010000000
VERSION     DAWHSK010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7217
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.088"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(35..760)
                     /locus_tag="WIC03_001136"
     CDS             complement(35..760)
                     /locus_tag="WIC03_001136"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEZ3883641.1"
                     /translation="MRIPLLAPDNYAFPDPAYALARCDGLVGVSRDLDAGRLLEAYQN
                     GVFPWFLRDGWFFWYAVGPRAVIVPERLHVPCSLAKTLRNGSYRVAVNGCFAEVVAHC
                     AAAARPNQDGTWIAPEFQTAYLKLHEMGYAHSFECHYPDENGKTRLAGGFYGVQIGRV
                     FYGESMFALQPDASKIAFACAVPFLADLGVELIDCQQDTEHMRRFGSELLPFADFAER
                     LRMLNAVPLKEEIGRREVACKGL"
     gene            complement(830..1264)
                     /gene="fur"
                     /locus_tag="WIC03_001137"
     CDS             complement(830..1264)
                     /gene="fur"
                     /locus_tag="WIC03_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HEZ3883642.1"
                     /translation="MEKFNNIAQLKDSGLKVTGPRLKILDLFETHAEEHLSAEDVYRI
                     LLEEGVEIGVATIYRVLTQFEQAGILQRHHFETGKAVYELDKGDHHDHIVCVKCGEVT
                     EFHNPEIEALQDKIAEENGYRIVDHALYMYGVCSDCQAKGKR"
     gene            1483..1860
                     /locus_tag="WIC03_001138"
     CDS             1483..1860
                     /locus_tag="WIC03_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEZ3883643.1"
                     /translation="MNKTLILALSALLGLAACSAERVSLFPSYKLKIIQGNELEPRAV
                     AALRPGMTKDQVLLLLGSPILRDAFHTDRWDYTFNTSRNGIIKERSNLTVYFENGVLV
                     RTEGDVLQNAAEALKDRQNTDKP"
     gene            1870..2679
                     /gene="dapB"
                     /locus_tag="WIC03_001139"
     CDS             1870..2679
                     /gene="dapB"
                     /locus_tag="WIC03_001139"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEZ3883644.1"
                     /translation="MTPLKIAIAGVNGRMGRVLVEAVNNHPDTVLSGALEHSGSEALG
                     LDAGYAVGLKTGIAISDDVDAVLAQSDVLIDFTRPEPTLKHLQKCVEKQVNIIIGTTG
                     FDDAGKAAIHTAAEQTGIVFAANFSVGVNLTFHILDTVARVLNEGYDIEIIEGHHRHK
                     VDAPSGTALRMGEVIAGALGRDLKQCAVYGREGHTGPRDPSTIGFATVRAGDIVGDHT
                     ALFATDGERVEITHKASSRMTFAAGAVRAAVWVNGKTGLYDMQDVLGLNNR"
     gene            complement(2793..3203)
                     /locus_tag="WIC03_001140"
     CDS             complement(2793..3203)
                     /locus_tag="WIC03_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883645.1"
                     /translation="MTTYTIPKKDYQFLYIYEGTLLNYTLKNDEFHIIVQNVDYPDFP
                     QEIPTPNYTDWVKIKFKQFSYLKFVYGYATKSQDINIDNTLELGELKQDDEILDYGGA
                     LELIGGRYDFPTAFRIDIVCREIELEFLDQEGFN"
     gene            complement(3205..3451)
                     /locus_tag="WIC03_001141"
                     /pseudo
     CDS             complement(3205..3451)
                     /locus_tag="WIC03_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448256.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3459..4613)
                     /gene="lpxB"
                     /locus_tag="WIC03_001142"
     CDS             complement(3459..4613)
                     /gene="lpxB"
                     /locus_tag="WIC03_001142"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEZ3883646.1"
                     /translation="MVDKKSPLIAVSVGEASGDLLGAHLIRAIRERCPQARFTGIGGE
                     LMKAEGFESLYDQERLAVRGFVEVVRRLPEILRIRRGLVRDLLSLKPDVFVGIDAPDF
                     NLGVAEKLKRTGIPTVHYVSPSVWAWRRERVGKIVHQVNRVLCLFPMEPQLYLDAGGR
                     AEFVGHPMAQLMPLEDDRETARKTLGVDAGIPVFALLPGSRVSEIDYMAPVFFQTALL
                     LLERYPAARFLLPAATAATKRRLAEVLQRPEFAGLPLTVIDRQSETVCRAADAVLVTS
                     GTATLEVALCKRPMVISYKISPLTYAYVKRKIKVPHVGLPNILLGKEAVPELLQHDAV
                     PEKLAAALADWYEHPDKVAALQQDFRALHLLLKKDTADLAARAVLEEAGC"
     gene            complement(4679..5671)
                     /locus_tag="WIC03_001143"
     CDS             complement(4679..5671)
                     /locus_tag="WIC03_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEZ3883647.1"
                     /translation="MKTHEISKDSVSLIGVAEHEAGQRLDNYLIKILKGVPKSHIHRI
                     IRAGEVRLNKKRCKPDSRIAEGDTVRIPPVRVAEKEMPSERRAAVPARAFDVVYEDDA
                     LLVIDKPSGVAVHGGSGVSFGVIEQLRRARPEAKYLELVHRLDKDTSGLLMVAKKRSA
                     LVKLHEAIRNDHPKKIYLALGVGKLPDDNFHVKLPLFKYTGAQGEKMVRVSADGQSAH
                     TVFRVLSRFSDGILHGVGLSHLTLVRATLKTGRTHQIRVHLQSQGCPIAGDERYGDYQ
                     ANRRLQKLGLKRMFLHASELHLNHPLTGEPLVLKVGLPPDLAQFAVMLENGTKM"
     gene            6164..>7217
                     /locus_tag="WIC03_001144"
     CDS             6164..>7217
                     /locus_tag="WIC03_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEZ3883648.1"
                     /translation="MKRMLFNATQAEELRVAIVDGQNLLDLDIETLGKEQRKGNIYKG
                     IITRIEPSLEACFVDYGTDRHGFLPFKEVSRSYFQDYEGGRARIQDVLKEGMEVIVQV
                     EKDERGNKGAALTTFISLAGRYLVLMPNNPRGGGVSRRIEGEERQELKAAMAELDIPN
                     GMSIIARTAGIGRSAEELEWDLNYLKQLWQAIEEAGKAHHDPYLLFMESSLLIRAIRD
                     YFRPDIGEILVDNQEVYDQVAEFMSYVMPGNIGRLKLYEDHTPLFSRFQIEHQIESAF
                     SRSVSLPSGGAIVIDHTEALVSIDVNSARATRGADIEDTAFKTNMEAAEEVARQMRLR
                     DLGGLVVIDFIDMENPK"
BASE COUNT         1763 a         2271 c         1658 g         1525 t
ORIGIN      
        1 ccatatttga acctgaccgg agcataagcc gccatcaaag ccccttgcac gccacttcgc
       61 gccgcccgat ttcctctttc aacggcacgg cgttcaacat ccgcagacgt tcggcaaaat
      121 ccgcaaacgg cagcagctcc gaaccgaaac ggcgcatatg ttccgtatcc tgctggcagt
      181 ctatcagttc cacgcccaaa tccgccaaaa acggcacggc gcaggcaaaa gcgattttcg
      241 acgcatccgg ttgtaatgcg aacatcgatt cgccgtaaaa caccctgccg atttgcacgc
      301 cgtaaaagcc gcccgccaac ctcgttttac cgttttcatc gggataatgg cactcgaaag
      361 aatgcgcgta ccccatttcg tgtagcttca aatatgccgt ctgaaactcg ggcgcaatcc
      421 aagttccgtc ctgattcggg cgcgccgctg ccgcacaatg cgcgaccact tccgcaaaac
      481 agccgttgac tgcaacccga tagctgccgt tgcgtagcgt tttcgccagc gagcacggaa
      541 cgtgcagacg ttcgggaaca atcaccgcac gcggcccgac cgcataccaa aaaaaccaac
      601 cgtcccgcaa aaaccaagga aacacgccgt tctgatacgc ctcgagcagc cgccccgcgt
      661 ccaaatccct gctcacgccg accagcccgt cgcaccgtgc caaagcatag gcaggatcgg
      721 gaaaggcata attgtcaggg gcaagcagcg gaatacgcat ggcagttctc caaaaacaca
      781 gacggcatcc aaaatcatga atgccgtctg aacaacaaac cgtccggatt taacgtttgc
      841 ccttggcctg acagtcgctg cacacgccgt acatataaag cgcgtgatcg acgatgcggt
      901 agccgttttc ctcggcgatt ttgtcttgca gggcttcgat ttcgggattg tggaattccg
      961 ttacctcgcc gcacttcacg cagacgatat ggtcatggtg gtcgcctttg tccaactcat
     1021 aaaccgcctt gcccgtttca aaatgatggc gttgcagaat acccgcctgc tcaaactggg
     1081 tcagcacacg gtaaatcgtc gccacaccga tttccacacc ctcttccaac aaaatgcggt
     1141 acacatcttc cgcactcaaa tgctcttccg catgcgtctc gaacaaatcc aaaatcttca
     1201 aacgcgggcc ggtaaccttc agaccgctgt ctttcagttg tgcaatattg ttgaattttt
     1261 ccataatatt caatacccct gtaaaacaat agacgttata atacgcaatt tcggcctgtt
     1321 tgcccactat cgcaccatag cagtttgcaa tagtaaaacc gcaccggcgg ccgatgcgcg
     1381 gacagggcgg ataccgctta atcgtatgat tatcgttcgc ttttataaaa tattcaagca
     1441 gtgttacact acccatcccg atttgcacag aaaggcattt ccgtgaacaa aaccctcatc
     1501 ctcgcccttt ccgccctcct cggccttgcc gcgtgcagtg ccgaacgcgt ttcactgttc
     1561 ccctcgtaca aactcaaaat catacagggc aacgaactcg aaccgcgcgc cgttgccgcc
     1621 ctccgccccg gcatgaccaa agaccaagtc ctgctcctgc tcggcagccc catactgcgc
     1681 gacgcattcc ataccgaccg ctgggactat accttcaaca cctcccgcaa cggcatcatc
     1741 aaagaacgca gcaatctgac cgtctatttt gaaaacggcg tactcgtccg caccgaaggc
     1801 gacgtcctgc aaaacgctgc cgaagccctc aaagaccgcc aaaacacaga caaaccataa
     1861 ggaacacaca tgacaccgct caaaatcgct atcgccggcg tcaacggccg catggggcgc
     1921 gtattggttg aagccgtcaa caaccatccc gacaccgtcc tttccggcgc gctcgaacac
     1981 tcgggctcag aagccctcgg gctggacgca ggctacgccg tcggactcaa aaccggcatc
     2041 gccatttcag acgacgttga cgccgttctc gcacaaagcg acgtactcat cgacttcacc
     2101 cgccccgagc ccaccctcaa acacctgcaa aaatgcgttg aaaaacaagt caacatcatc
     2161 atcggcacaa caggcttcga cgatgcgggc aaagccgcta tccacactgc cgccgaacaa
     2221 acaggcatcg ttttcgccgc caacttcagc gtcggcgtca acctcacctt ccacatcctc
     2281 gacaccgtcg cacgcgtatt aaacgaaggc tacgacatcg aaatcattga aggccaccac
     2341 cgccacaaag tcgatgcccc cagcggcacc gcgttacgca tgggcgaagt catcgccggc
     2401 gcgctcggca gagacctcaa acaatgcgcc gtttacggcc gcgaaggcca caccggtccg
     2461 cgcgatccgt cgaccatcgg ctttgccacc gtccgcgcag gcgacatcgt cggcgaccac
     2521 accgccctct tcgccaccga cggcgagcgc gtggaaatca cccacaaggc cagcagccgc
     2581 atgacctttg ccgccggtgc cgtccgcgcc gcagtttggg taaacggcaa aacgggtttg
     2641 tacgatatgc aggacgtact cgggctgaac aaccgttaac ccccatacaa aatgccgtct
     2701 gaaaagatat tgttcagacg gcattttgcc aacaggctcc gtatcggcat atcaataaag
     2761 ttgcttgcgc ggcatgacaa gctacggctc gtttaattga aaccctcctg atctaaaaat
     2821 tctaactcta tttcccggca aactatatct atcctaaaag cagtcggaaa atcataccta
     2881 ccgcctatca attccagcgc acctccataa tccaagattt catcatcctg ctttaattcg
     2941 ccaagttcca atgtattatc gatatttata tcttggctct ttgtagcgta tccatagaca
     3001 aatttcagat agctgaactg cttgaattta atttttaccc agtctgtata atttggtgta
     3061 ggaatctctt gaggaaagtc cggataatcc acattctgga cgatgatatg gaattcatcg
     3121 tttttcaaag tatagtttaa tagagtgccc tcatatatat acagaaattg ataatctttt
     3181 tttggaatgg tataagttgt catattattt taccttttca aaaacaatga gtttaagatt
     3241 ttttgagtcc aagttggttc tactactctt atgaaatttc agttgtttaa actcttcctt
     3301 taacggccca ttatatttta accttaatcc cactcgagcc agttctcctt catttggaat
     3361 tcctcttaaa tacttattgt ttgatgtgcc attaatcaca atttctcccc cctttttttg
     3421 taatacgtgc tgcttcaaga agataatcca ttaaccgctc aacatcccgc ctcttccaaa
     3481 accgcgcgcg cggccaaatc cgccgtatct tttttcaaca gcaggtgcaa cgccctgaaa
     3541 tcctgttgca gcgcggcaac cttatcgggg tgttcgtacc agtccgccaa cgccgccgcc
     3601 agtttttccg gtacggcatc atgttgaagc agctccggca cagcctcctt acccaacagg
     3661 atattcggca ggccgacatg cggcactttg attttgcgtt tcacataagc ataagtcagc
     3721 ggcgaaatct tgtagctgat gaccatcgga cgcttgcaca gagccacttc caaagttgcc
     3781 gtaccgctcg ttaccagcac cgcatccgcc gccctgcaca ctgtttcaga ctgtctgtcg
     3841 attaccgtca gcggcaatcc ggcaaactcc ggccgctgca aaacttccgc caaacgccgc
     3901 ttcgtcgccg ccgttgcggc aggcagcagg aagcgtgcgg caggatagcg ttccaacaac
     3961 aataatgccg tctgaaaaaa caccggcgcc atatagtcga tttcgctgac gcggctgccg
     4021 ggcagcaggg cgaatacggg gatgccggca tccacgccca aagttttccg cgccgtttca
     4081 cggtcgtctt ccaagggcat aagctgcgcc atcggatgac cgacaaactc cgcacgtccg
     4141 cccgcatcga gataaagctg cggctccatc gggaacaggc acagcacgcg gttgacctga
     4201 tgcacgattt tgcccacgcg ttcccgccgc cacgcccaca ccgacggact gacataatgc
     4261 acggtcggaa tgcccgtccg tttcagcttt tccgccacac ccaaattgaa atcgggcgca
     4321 tcgataccga caaagacatc aggtttcaac gacagcaaat cccgtaccag ccccctgcgt
     4381 atccgtaaaa tttccggcag ccgcctgacc acttcgacaa agccgcgcac cgccagccgc
     4441 tcctgatcat aaaggctctc gaaaccttcc gccttcatca gttccccgcc gataccggta
     4501 aaccgcgcct gcgggcaacg ctcgcggatg gcgcgtatca ggtgcgcccc caacaggtcg
     4561 cccgacgctt cgccgacact gacggcaatc aaagggcttt ttttatcaac catattcgtt
     4621 cttcttcaca tactttcacg tcccgatgcc gtctgaaggc ttcagacggc atcggggttc
     4681 acattttcgt cccgttttcc aacatcaccg caaactgtgc caaatccggc ggcagcccca
     4741 cctttaacac cagcggctcg cccgtgagcg gatggttcag gtgcagctcg gacgcgtgca
     4801 aaaacatccg cttcaaaccc aacttctgca aacgacggtt cgcctgataa tcgccgtagc
     4861 gttcgtcgcc cgcaatcgga cagccttgag attgcaggtg gacgcggatt tggtgcgtgc
     4921 gccccgtttt caacgtcgcc cgcaccaaag tcaggtgcga cagcccgaca ccgtgcaaaa
     4981 tgccgtctga aaaacggctt aacacacgga acaccgtatg cgccgactgc ccgtccgcac
     5041 tgacgcgcac catcttttcg ccttgtgcgc cggtatattt gaacaggggc agtttgacat
     5101 ggaaattgtc gtccggcagt ttgcccaccc ccagcgcaag gtagattttt ttggggtggt
     5161 cgttacggat ggcttcgtga agtttgacga gcgcgctgcg tttcttcgcc accatcaaca
     5221 agccgctcgt atccttgtcc aaacgatgaa ccaactccaa atacttcgcc tccggacggg
     5281 cgcggcgcaa ctgttcgata acgccgaaac tcacgccgct gccgccgtgg acggcaacgc
     5341 cggacggttt gtcgatgacc aaaagcgcat cgtcttcgta aacaacgtca aacgcacgcg
     5401 ccggtacggc ggcacgcctt tcagacggca tttccttctc cgccacgcgc acaggcggaa
     5461 tccgcaccgt atccccctcc gcaatacggc tgtcgggttt gcagcgtttc ttgttcaacc
     5521 gcacctcgcc ggcgcggata atgcggtgga tatggctctt gggaacaccc ttgaggattt
     5581 ttatcagata gttatcaagg cgttgacccg cctcatgttc ggcaacccct atcaagctga
     5641 ccgaatcttt gcttatttcg tgcgttttca tctataatcc gaacatccgt ttcagcaaaa
     5701 agcgcgcccg ccgcgcctcc tgaaacggtt tactttaaaa cggaatttta tattaaaaac
     5761 gcactccgca aagcattttc cctgcgcctg ttcccagccg gcaggcgcag aacgtaatca
     5821 agtttgaatt gattttgccg tttcggcgcg gaagtaagac ggcagccggg cccaagtccc
     5881 aaacgcaaac cgcccaaaac acaggcatca cgataacaag aagaagccgg cccccatttt
     5941 tccgaccgca gcgttcggaa cgcggcagac aaaccgccgc atcgatcttt atagccttct
     6001 gtatccgacc cttccgcaca cggttctccg gaacgtgcat ccctgcggat tttcaactca
     6061 aaaactgatt accggtttta tccgaaaaca tcggaaaacc cagccttacg gaatgccgaa
     6121 ccgggcatgt gccgtctgaa cgccgcctgc ccacgaggtg atcatgaaaa gaatgttatt
     6181 taacgcaacg caggccgaag agctgcgcgt tgccatcgtc gacggacaaa acctgctgga
     6241 tttggacatc gaaacgctgg gcaaagaaca gcgcaaaggc aatatctaca aaggcatcat
     6301 tacccgcatc gagccgtcgc tggaagcgtg tttcgtggat tacggaaccg accgccacgg
     6361 cttcttgccg tttaaagaag tctcccgctc atacttccaa gactacgaag gcggacgcgc
     6421 gcgcatccaa gacgtgctca aagaaggcat ggaagtcatc gtccaagtcg aaaaagacga
     6481 gcgcggcaac aaaggcgccg cgctgaccac cttcatcagc ctcgccggac gctatctggt
     6541 attgatgccg aacaacccgc gcggcggcgg cgtatcccgc cgtatcgaag gtgaagagcg
     6601 tcaagagttg aaagccgcca tggcggaact cgacattccg aacggcatga gcatcatcgc
     6661 ccgtaccgcc ggcatcggcc gcagcgcgga agagttggaa tgggacttga actacctcaa
     6721 acaactctgg caggcgattg aagaagcagg aaaagcgcat cacgacccct acctgctctt
     6781 tatggaaagc tcgctgctga tccgagccat ccgcgactat ttccgccccg acatcggcga
     6841 gattttggtg gacaatcaag aagtttacga ccaagttgcc gagttcatga gctacgtcat
     6901 gccgggcaat ataggccgtc tgaaactcta cgaagaccac acgccgctgt tttcccgctt
     6961 ccaaatcgaa caccaaatcg aaagcgcgtt ttcgcgcagc gtcagcctgc cctccggcgg
     7021 cgcgatcgtc atcgaccata ccgaagccct cgtctccatc gacgtgaact ccgcacgcgc
     7081 cactcgcggc gcagacattg aagacaccgc gttcaaaacc aatatggaag ccgccgaaga
     7141 agtcgcccga caaatgcgcc tgcgcgactt gggcggcttg gtcgtcatcg acttcatcga
     7201 catggaaaac cccaaac
//
LOCUS       DAWHSK010000089         7177 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.089, whole genome shotgun sequence.
ACCESSION   DAWHSK010000089 DAWHSK010000000
VERSION     DAWHSK010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7177
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.089"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(92..880)
                     /locus_tag="WIC03_001145"
     CDS             complement(92..880)
                     /locus_tag="WIC03_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEZ3883649.1"
                     /translation="MNPQDKCWQVHRHLAEHADQRLTLVRNAPKHILLAGADADISRS
                     LLAKRYPQAVFEEYDSRADFLAAAAAVRKGGFWQRFTGKGVVQHCQSPTAPLPEACAD
                     MLWSNLGLLAAEQILPVLHNWARALETDGLLFFTCFGRDTFTELKCRLKENGIESRSV
                     MFPDMHDLGDMLAENGFYDPVTDTAKLVLDYKKAETFWADMDTLGVWRAMSWNDENAA
                     RSCVGTIFEREGGLSITLETVYGHAVKKLMLPQGENVVQFFPKR"
     gene            complement(882..1796)
                     /gene="bioH"
                     /locus_tag="WIC03_001146"
     CDS             complement(882..1796)
                     /gene="bioH"
                     /locus_tag="WIC03_001146"
                     /EC_number="3.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HEZ3883650.1"
                     /translation="MCLKHRGQLFAASVRYAAKSSRQPAQMPSETPDEPWHNTQRLIV
                     GASAMRRQRERKSMPDAVKKVYLIHGWGANRHMFDDLMPRLPATWPVSAVDLPGHGDA
                     PFAQPFDIEAAADAVAAQIDTPADILGWSLGGLVALYLAARHPDKVRSLCLTASFARL
                     TADEDYPEGLAAPALGKMVGAFRSDYAKHIKQFLQLQLLHTPDADGIIGRILPDLARC
                     GTPSALQEALDAAERADARHLLDKIDVPVLLVFGSKDAITPPRMGEYLHRRLKGSRLV
                     VMEKAAHAPFLSHAEAFAALYRDFVEGV"
     gene            1741..2349
                     /locus_tag="WIC03_001147"
     CDS             1741..2349
                     /locus_tag="WIC03_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEZ3883651.1"
                     /translation="MAAYRTDAANSCPLCFRHIQGGAVCGGCQKKPPAFDRMWASLHY
                     EPPVSNMIRTLKHLADLGMVRPLANLMVQNPPDRLADECFDFVLPVPLSRERLLQRGF
                     NQSESIVGLLAQRYGWQILPRHTVFRHHRPPQSTLKGSERRRNIKNAFEIRTPIPENC
                     NILLIDDVFTTGATLDELAKTLKKSGANRICCWTLARTPMKK"
     gene            2414..2878
                     /gene="trmL"
                     /locus_tag="WIC03_001148"
     CDS             2414..2878
                     /gene="trmL"
                     /locus_tag="WIC03_001148"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HEZ3883652.1"
                     /translation="MFTIVLYQPEIPPNTGNIIRLCANTGADLHLVKPLGFPLDSAKM
                     KRAGLDYHEFASLTVHENFDDCLKALAGRRIFALTTKGTARPDETAFQKGDVLLFGPE
                     TRGLPADILDSLPAAQKIRLPMQPGSRSMNLSNTVAVILFEAWRQHGYAGGV"
     gene            2889..3011
                     /locus_tag="WIC03_001149"
     CDS             2889..3011
                     /locus_tag="WIC03_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEZ3883653.1"
                     /translation="MPSENLSGHIPNRRPHCTAVRNVSGGHDAHSSSTQERNTF"
     gene            3008..3727
                     /locus_tag="WIC03_001150"
     CDS             3008..3727
                     /locus_tag="WIC03_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEZ3883654.1"
                     /translation="MTLTRKTLFLLTAAFGTHSLQTASADAVVKAEKLHASANRSYKV
                     AGKRYTPKNQVAEFTQTGNASWYGGRFHGRKTSGGERYDMNAFTAAHKTLPIPSYVRV
                     TNTKNGKSVIVRVNDRGPFHGNRIIDVSKAAAQKLGFVNQGTAHVKIEQIVPGQSAPV
                     AENKDIFIDLKSFGTEHEAQAYLNQAAQNFAASASSPNLAIEKRRYEYVVKMGPFASQ
                     ERAAEAEAQARGMVRAVLTAG"
     gene            3781..4107
                     /locus_tag="WIC03_001151"
     CDS             3781..4107
                     /locus_tag="WIC03_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEZ3883655.1"
                     /translation="MNFKRLLLTAAATALMGISAPALAHHDGHGDDDHGHAAHQHSKQ
                     DKIISRAQAEKAALARVGGKITDIDLEHDNGRPHYDVEIVKNGQEYKVVVDARTGRVI
                     SSRRDD"
     gene            4189..4773
                     /gene="ruvA"
                     /locus_tag="WIC03_001152"
     CDS             4189..4773
                     /gene="ruvA"
                     /locus_tag="WIC03_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEZ3883656.1"
                     /translation="MISRLTGKLVEKNPPQIVIDVNGVGYEADVSMQTFYNLPPVGES
                     VQLFTQLIIREDAHLLFGFATAEERKTFRQLIKVGGIGAKTALGILSAMTADELAQAV
                     AEEDVKRLSSAPGIGKKTAERMVLELRGKLVAHTVTDGLFAAAPAADETEDIVSTLLA
                     LGYSEREAKAAVKGVPEGTDVGEGVRLALKNLLK"
     gene            4773..4910
                     /locus_tag="WIC03_001153"
     CDS             4773..4910
                     /locus_tag="WIC03_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883657.1"
                     /translation="MPSEGGATFALTKPCVSASRCLPDGFSRKMALLSFLSDGIVFDA
                     V"
     gene            4936..6792
                     /locus_tag="WIC03_001154"
     CDS             4936..6792
                     /locus_tag="WIC03_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ3883658.1"
                     /translation="MLNKIFSWFESRIDPYPEAAPKTPEKGLWRFIWSNMAGVRKWIA
                     ALAALTAGIGIMEALVFQFMGKIVEWLGKYAPAELFAEKGWELAAMAAMMVFSVVWAF
                     AASNVRLQTLQGVFPMRLRWNFHRLMLNQSLGFYQDEFAGRVSAKVMQTALALRDAVM
                     TVADMVVYVSVYFITSGVILASLDSWLLLPFIGWIVGFASVMRLLIPKLGQTAAWQAD
                     ARSLMTGRITDAYSNIATVKLFSHGAREAAYAKQSMEEFMVTVRAQMRLATLLHSCSF
                     IVNTSLTLSTAALGIWLWHNGQVGVGAVATATAMALRVNGLSQYIMWESARLFENIGT
                     VGDGMATLSKPHTILDKPNALPLKVSQGEIKFEHVDFSYEAGKPLLNGFNLTIRPGEK
                     VGLIGRSGAGKSTIVNLLLRFYEPQSGTVSIDGQDISGVTQESLRAQIGLVTQDTSLL
                     HRSVRDNIIYGRPDATDAEMVSAAERAEAAGFIPELSDAKGRRGYDAHVGERGVKLSG
                     GQRQRIAIARVMLKDAPILLLDEATSALDSEVEAAIQESLDKMMDGKTVIAIAHRLST
                     IAAMDRLVVLDKGRIIEEGTHAELLEKRGLYAKLWAHQSGGFLNEHVEWQHD"
     gene            6797..>7177
                     /locus_tag="WIC03_001155"
     CDS             6797..>7177
                     /locus_tag="WIC03_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase"
                     /protein_id="HEZ3883659.1"
                     /translation="MPSEHPFSDGISTPNPKETMNDTAQITASYGRRYIVRTPDGTTY
                     EASTRKKRVDFACGDRVRISPVNAEQVVIEDFLPRQSLLYRQDAWKTKLIAANVTQLL
                     IVTAAVPSPSMRLLQRALLAAEAAG"
BASE COUNT         1635 a         2290 c         1880 g         1372 t
ORIGIN      
        1 cactatacat cggcatttcc tttagttctg tcggttttca caaacaaatg ccgtctgaaa
       61 cttggaaacg gcttcagacg gcatctgcac atcatctctt cggaaaaaac tgcaccacgt
      121 tctccccttg cggcagcatc agttttttaa cggcgtgtcc gtacactgtt tccagcgtaa
      181 tactcaaacc gccttcccgt tcaaatattg tcccgacaca cgaacgcgcg gcgttttcat
      241 cgttccacga catcgcccgc caaacgccca gcgtgtccat atccgcccaa aacgtttccg
      301 cctttttgta atccaacacc agcttcgccg tatcggtaac ggggtcgtaa aacccgtttt
      361 cagcaagcat atcgcccaag tcgtgcatat cgggaaacat cacgctgcgg ctttcaatgc
      421 cgttttcttt cagacggcat ttcagttcgg taaaagtgtc gcgcccgaag caggtaaaaa
      481 acagcagccc gtccgtctcc agcgcgcgcg cccagttgtg cagcacagga aggatttgtt
      541 ccgccgccaa cagtccgaga ttcgaccaca acatatcggc acacgcttcg ggcagcggcg
      601 cggtcgggga ttggcagtgt tgcaccacgc ccttacccgt aaacctttgc caaaaaccgc
      661 ccttgcgcac ggctgcggca gccgccaaaa aatccgcacg ggaatcgtat tcttcaaata
      721 ccgcctgcgg atatcgtttc gccagcaggc tgcggctgat gtccgcatcc gccccggcaa
      781 gcaggatatg tttgggcgcg ttgcggacga gtgtcagccg ttggtcggca tgttcggcaa
      841 gatggcggtg aacctgccag catttgtcct gcggattcat gtcaaacccc ttcgacaaag
      901 tcgcggtaca gcgcggcaaa cgcttccgca tggctcaaaa acggcgcgtg tgccgccttt
      961 tccatcacaa ccagcctgct gcctttcaaa cggcggtgca gatactcgcc catgcgcggc
     1021 ggcgtaatcg cgtctttgct accgaacacc agcagtaccg gaacatctat cttgtccaac
     1081 aaatgccgcg catccgccct ttccgccgcg tccaaagctt cctgtaaggc ggagggcgtg
     1141 ccgcagcgcg ccaaatcggg caggattctg cctatgattc cgtccgcatc aggcgtgtgc
     1201 agaagctgga gttgtagaaa ctgtttgata tgtttggcat aatccgaacg gaacgcgccg
     1261 accattttgc ccaatgcagg cgcggcaagc ccttcgggat agtcttcgtc agccgtcagt
     1321 cgggcgaaac tcgccgtcag gcagagcgaa cggactttgt cgggatggcg cgccgccaga
     1381 tacagcgcga ccaatccgcc gagcgaccag ccgagaatgt cggcgggcgt atcgatttgt
     1441 gcggcaacag cgtcggcggc tgcctcaata tcgaagggtt gcgcgaaggg cgcgtcgccg
     1501 tgtccgggca aatcgacggc ggacaccggc cacgttgcag gcaggcgcgg catcaaatcg
     1561 tcgaacatgt ggcggttcgc cccccaaccg tgtatcaggt aaactttttt gacggcatca
     1621 ggcatggatt ttctctctcg ttggcggcgc atcgcagacg cgcccactat caggcgttgc
     1681 gtattatgcc acggttcgtc cggcgtttca gacggcatct gcgccggctg ccgcgacgat
     1741 ttggcggcat accgcaccga cgcggcaaac agctgccccc tgtgtttcag gcacatccaa
     1801 ggcggcgcgg tgtgcggcgg ctgtcagaaa aaaccgcccg cattcgaccg gatgtgggct
     1861 tcgctgcatt acgaaccgcc cgtcagcaac atgatacgta cgctgaaaca cttggcagat
     1921 ttgggcatgg ttcgcccatt ggcaaacctg atggtacaaa acccgcccga ccggcttgca
     1981 gacgaatgtt tcgatttcgt cctgcccgta ccgctaagca gggagcggct gctgcaacgc
     2041 gggttcaacc aaagtgaaag catcgtcggg ttgttggcac aacgctacgg ctggcagata
     2101 ctgccccgac acaccgtatt ccgacaccac cgcccgccgc aaagcacgct caaaggcagc
     2161 gaacggcggc gaaacatcaa aaacgccttt gaaatccgca caccgatacc ggaaaactgt
     2221 aatattctgt taatcgacga tgtctttacc accggcgcga cgctggacga actggcaaag
     2281 acgctgaaaa aatcgggcgc gaaccgcatc tgctgctgga cgctggcacg cacgccaatg
     2341 aaaaaataac taaatttttt gacaccccat ccgccttgtg gcaaggttac gcgcctataa
     2401 gtgattgata tttatgttta ccatcgtttt ataccagccg gaaatcccgc cgaacacggg
     2461 caacatcatc cgcctgtgcg ccaataccgg cgcggatttg caccttgtca aaccgctcgg
     2521 cttcccattg gattccgcca aaatgaaacg cgccgggctc gactaccacg aattcgccag
     2581 cctgacggtg cacgaaaact tcgacgactg cctcaaggca ctcgcaggca ggcgcatttt
     2641 cgccctgacc accaaaggca cggcgcgccc cgatgaaacc gcgtttcaaa aaggcgacgt
     2701 tttactgttc gggccggaaa cgcgcggact gcctgccgac atcctcgaca gcctgcccgc
     2761 cgcgcaaaaa atccgcctgc ccatgcagcc cggcagtcgg agtatgaacc tttccaatac
     2821 cgttgccgtg attctctttg aagcgtggcg gcaacacggt tacgcaggcg gcgtttgaac
     2881 gcaagttcat gccgtctgaa aacctatccg gacacattcc gaaccgccgt ccgcactgta
     2941 cggcggttcg gaacgtatcc ggcgggcatg atgcccattc gtcttcaact caagaacgga
     3001 acacgttttg actttaaccc gaaaaaccct tttccttctc accgccgcat tcggcacaca
     3061 ctcccttcag acggcatccg ccgacgcagt ggtcaaggca gaaaaactgc acgcctccgc
     3121 caaccgcagc tacaaagtcg ccggaaaacg ctacacgccg aaaaaccaag tcgccgaatt
     3181 cacgcaaacc ggcaacgcct cgtggtacgg cggcaggttt cacgggcgca aaacttccgg
     3241 cggagaacga tacgatatga acgcctttac cgccgcccac aaaaccctgc ccatccccag
     3301 ctatgtgcgc gtaaccaata ccaaaaacgg caaaagcgtc atcgtccgcg tcaacgaccg
     3361 cggccccttc cacggcaacc gcatcatcga cgtatccaaa gccgccgcgc aaaaattggg
     3421 ctttgtcaac caagggacgg cgcacgtcaa aatcgaacaa atcgtcccgg gccaatccgc
     3481 accggttgcc gaaaacaaag acatcttcat cgacttgaaa tctttcggta cggaacacga
     3541 agcacaagcc tatctgaacc aagccgccca aaacttcgcc gcttcggcat caagcccgaa
     3601 cctcgccatt gaaaaacgcc gttacgaata cgtcgtcaaa atgggaccgt ttgcctcgca
     3661 ggaacgcgcc gccgaagccg aagctcaggc gcgcggtatg gttcgggcgg tattgactgc
     3721 cggttgatgt cccgccttta cgtctttcaa acaccaccag atacttcgca ggagcaaaac
     3781 atgaacttca aacgccttct cttgaccgcc gccgcaaccg cactgatggg catttccgcc
     3841 cccgcactcg cccaccacga cggacacggc gatgacgacc acggacacgc cgcacaccaa
     3901 cacagcaaac aagacaaaat catcagccgc gcccaagccg aaaaagcagc gttggcgcgt
     3961 gtcggcggca aaatcaccga catcgatctc gaacacgaca acggccgtcc gcactatgat
     4021 gtcgaaatcg tcaaaaacgg acaggaatac aaagtcgttg tcgatgcccg taccggccgc
     4081 gtgatttcct cccgccgcga cgactgaatt tgatacaatc cgtgccgtct gaagcccgaa
     4141 ccggtttcag acggcatttt gcacccgaca cttcaggatt cggcatacat gatcagcaga
     4201 ctgaccggca aactggttga aaaaaaccct ccgcaaatcg tcatcgatgt caacggtgtc
     4261 ggctatgagg ccgacgtatc gatgcagacc ttctacaacc tgccgcccgt gggtgaaagc
     4321 gtacaactgt ttacccagct tatcattcgg gaagacgcac atcttttatt tggttttgcc
     4381 actgcggaag aacgcaaaac attccgccaa ctgatcaagg tcggcggcat cggcgcgaaa
     4441 acggctttgg gcattttgtc ggcgatgacg gcagacgagc tggcgcaggc ggttgcagaa
     4501 gaagacgtga aacgcctgtc ttccgcgccg ggaatcggca aaaaaaccgc cgaacgtatg
     4561 gtcttggaac tgcgcggcaa gctggtcgcg catacggtaa cggacgggct gtttgccgcc
     4621 gcacccgccg ccgacgaaac ggaagacatc gtcagcacgc tgcttgcgct gggttacagc
     4681 gaacgcgaag caaaagcggc ggtcaaaggc gttccggagg ggacggacgt gggcgaaggc
     4741 gtgcgccttg ctttgaaaaa cctgctgaaa taatgccgtc tgaaggcggc gcgacgtttg
     4801 ccctgacgaa accctgcgtt tccgcctcgc gctgtcttcc cgacggcttt tcccgtaaaa
     4861 tggcgttatt gtccttcctt tcagacggca ttgtgtttga tgccgtctga aactgtttta
     4921 ccgaaatcga aaacaatgtt gaacaaaatc ttttcctggt tcgaatcccg aatcgacccc
     4981 tatcccgaag ccgccccgaa aacgcctgaa aaaggactgt ggcggtttat ctggagcaac
     5041 atggctgggg tgcggaaatg gatagccgcc ctggctgcgc tgaccgccgg catcggcatt
     5101 atggaagccc tggtttttca atttatgggc aaaatcgtgg agtggctcgg caaatacgcg
     5161 cccgccgaac tgtttgccga aaaaggttgg gaactggcgg caatggcggc gatgatggtg
     5221 ttttcggtcg tgtgggcgtt tgccgcgtcc aacgtgcgcc tgcaaaccct tcagggcgtg
     5281 ttccccatgc gcctgcgctg gaacttccac cgcctgatgc tgaaccaaag cctcggtttt
     5341 tatcaggacg aatttgccgg acgcgtgtcc gccaaagtca tgcagaccgc gctggcgttg
     5401 cgcgacgcgg tgatgacggt tgccgatatg gtcgtttatg tgtcggtgta tttcattacc
     5461 tccggcgtga ttctcgcctc gctcgactca tggctgctgc tgccctttat cggctggatt
     5521 gtcggtttcg cttcggtgat gcgcctgctg attcccaaat tggggcaaac cgccgcatgg
     5581 caggcggatg cccgctcgct gatgaccggc cgcattaccg atgcctattc caatatcgcc
     5641 accgtcaaac tcttctccca cggcgcgcgt gaagccgcct atgccaagca gtcgatggaa
     5701 gaatttatgg ttacggtgcg cgcccaaatg cggctggcga cgctgctgca ttcgtgcagc
     5761 ttcatcgtca acacctccct gaccctctcc accgccgcac tgggcatctg gctctggcac
     5821 aacgggcagg tcggcgtggg cgcggttgct acagccaccg ccatggcgtt gcgcgtcaac
     5881 ggtttgtcgc aatacattat gtgggaatcc gcgcggctgt ttgaaaacat cggcaccgtc
     5941 ggcgacggca tggcaaccct gtccaaaccg cacaccatcc tcgacaagcc gaacgccctg
     6001 ccgctgaaag tgtcgcaagg cgaaatcaag ttcgaacacg tcgatttctc ctacgaagcg
     6061 ggcaaaccgc tgctcaacgg cttcaacctc accatccgcc cgggcgaaaa agtcggcttg
     6121 atcggacgca gcggcgcggg caaatccacc atcgtcaacc tgcttttgcg cttctacgaa
     6181 ccgcaaagcg gcacggtttc gatcgacggg caggacataa gcggcgttac ccaagaatct
     6241 ttacgcgccc aaatcggttt ggtcacgcaa gatacctcgc tgctgcaccg ttccgtgcgc
     6301 gacaacatta tttacggccg ccccgacgcg accgatgccg aaatggtttc cgccgccgaa
     6361 cgcgccgaag ccgccggctt catccccgaa ctttccgatg ccaaagggcg gcgcggctat
     6421 gacgcgcacg tcggcgaacg cggcgtgaaa ctctccggcg ggcaacgcca gcgcatcgcc
     6481 attgcccgcg tgatgctcaa agacgcgccc atcctgctgc tcgacgaagc caccagcgcg
     6541 ctcgattccg aagtcgaagc cgccatccag gaaagcctcg acaaaatgat ggacggcaaa
     6601 accgtcatcg ccatcgccca ccgcctctcc accatcgccg caatggacag gctcgtcgtc
     6661 ctcgacaaag gccgcatcat cgaagaaggc acacacgccg aactcctcga aaaacgcggg
     6721 ctttacgcca aactctgggc gcaccagagc ggcggcttcc tcaacgaaca cgtcgagtgg
     6781 cagcacgact gaaccgatgc cgtccgaaca cccgttttca gacggcattt ccacacccaa
     6841 ccccaaagaa accatgaacg acaccgccca aattaccgcc agctacggcc gccgctacat
     6901 tgtccgcacg cccgacggca caacctacga agccagcacc cgcaaaaaac gcgtcgattt
     6961 cgcctgcggc gaccgcgtcc gcatcagccc cgtcaacgcc gaacaagttg tgattgaaga
     7021 ttttttaccg cgccaaagcc tgctctaccg ccaagacgcg tggaaaacca aactcatcgc
     7081 cgccaacgtt acccaactcc tcatcgtaac cgccgccgtc ccgagtccga gcatgcggct
     7141 gctacaacgc gccctgcttg ccgccgaagc cgccggc
//
LOCUS       DAWHSK010000090         7164 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.090, whole genome shotgun sequence.
ACCESSION   DAWHSK010000090 DAWHSK010000000
VERSION     DAWHSK010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7164
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.090"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(93..452)
                     /gene="rnpB"
                     /locus_tag="WIC03_001156"
     ncRNA           complement(93..452)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="WIC03_001156"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(531..1634)
                     /gene="prfB"
                     /locus_tag="WIC03_001157"
     CDS             complement(531..1634)
                     /gene="prfB"
                     /locus_tag="WIC03_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEZ3883660.1"
                     /translation="MEAEVINQLNNTLNDLEKRSEDIRVYMDYQGKKDRLEEVVGLSE
                     DPELWNDPKRAQEIGKERKILEGIVLTLDNIATGIEDNRMLIEMTVEENDEEGFAAVQ
                     EDVAGLEKQMADLEFKRMFNQPADPNNCFIDITAGAGGTEAEDWAGMLFRMYSRYAER
                     KGFKIEILEEDDGEIAGINRATIRVEGEYAYGLLRTETGVHRLVRYSPFDSNNKRHTS
                     FASVFVYPEIDDSIEIEINPADLRIDTYRASGAGGQHINKTDSAVRITHEPTGIVVQC
                     QNDRSQHANKAAAMEMLKSKLYELEMRKRNEEKQALEEGKSDVGWGSQIRSYVLDSSR
                     IKDLRTGYEVGNTKAVLDGDLDGFIEASLKQGV"
     gene            complement(1737..2630)
                     /locus_tag="WIC03_001158"
     CDS             complement(1737..2630)
                     /locus_tag="WIC03_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEZ3883661.1"
                     /translation="MVTFSKIRPLLAIAAAALLAACGTAGNNAARKPVQTAKPAAVVG
                     LALGGGASKGFAHVGIIKVLKENGIPVKVVTGTSAGSIVGSLFASGMSPDRLELEAEI
                     LGKTDLVDLTLSTSGFIKGEKLQNYINRKVGGRRIQQFPIKFAAVATDFETGKAVAFN
                     QGNAGQAVRASAAIPNVFQPVIIGRHTYVDGGLSQPVPVSAARRQGANFVIAVDISAR
                     PGKNISQGFFSYFDQTLNVMSVSALQNELGQADVVIKPQVLDLGAVGGFDQKKRAIRL
                     GEEAARAALPEIKRKLAAYRY"
     gene            2972..3421
                     /locus_tag="WIC03_001159"
     CDS             2972..3421
                     /locus_tag="WIC03_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEZ3883662.1"
                     /translation="MKIGTTWQTASAMLVLRLFAAYEFLESGLQKWNGENWFSEINDQ
                     FPFPFNLLPDALNWNLAMYAELLLPVLLLLGLATRLSALGLMVVTAVAWAAVHAGSGY
                     NVCDNGYKMALIYIVVLIPLLFQGAGGWSLDTLLKKRFCPQCRLKQD"
     gene            3495..3803
                     /locus_tag="WIC03_001160"
     CDS             3495..3803
                     /locus_tag="WIC03_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883663.1"
                     /translation="MNKNIAAALAGALSLSLAAGAVAANKPASNATGVHKSAHGSCGA
                     SKSAEGSCGAAGSKAGEGKCGEGKCGATVKKTHKHTKASKAKAKSAEGKCGEGKCGSK
                     "
     gene            3919..4761
                     /locus_tag="WIC03_001161"
     CDS             3919..4761
                     /locus_tag="WIC03_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HEZ3883664.1"
                     /translation="MIQHAGLGYRRDLAEDFLSLSENSPICFIEAAPENWLKMGGWAR
                     KQFDRVAERLPLALHGLSMSLGGQAPLDTDLIDGIKEMMRRYDCTFFSDHLSYCHDGG
                     HLYDLLPLPFTEEMVHHTARRIREVQDRLGCRIAVENTSYYLHSPLAEMNEVEFLNAV
                     AREADCGIHLDVNNIYVNAVNHGLLSPEAFLKNVDADRVCYIHIAGHDVETPELLIDT
                     HGAAVLPTVWDLLELAYAKLPTIPPTLLERDFNFPPFAELEAEVAKIADYQTRAGKEC
                     RRAA"
     gene            4751..5497
                     /locus_tag="WIC03_001162"
     CDS             4751..5497
                     /locus_tag="WIC03_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2063 domain-containing protein"
                     /protein_id="HEZ3883665.1"
                     /translation="MQPETSAQYQHRFAQAIRGGEAADGLPQDRLNVYIRLIRNNIHS
                     FIDRCYTETRQYFDSEEWSRLKEGFVRDAHAQTPYFQEIPGEFLQYCQSLPLSDGILA
                     LMDFEYTQLLAEVAQIPDIPDIHYSNHSKYTPSPAAFIRQYRYDVTDDLQEAETALLI
                     WRNAEDDVMYQTLDGFDMMLLEIMGSSALSFDTLAQTLVEFMPEDDNWKNILLGKWSG
                     WTEQRIIIPSLSAISENMEGNSPGQNHLSA"
     gene            5519..6106
                     /locus_tag="WIC03_001163"
     CDS             5519..6106
                     /locus_tag="WIC03_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEZ3883666.1"
                     /translation="MPLPDLTDAELIESRKLLLHFARLQLPDHPDLAEDLVQETLLSA
                     YSAGDSFQGRALVNSWLFAILKNKIIDALRQIGRQRKVFTTLDDELLDEAFESHFSQN
                     GHWTQEGQPQHWNTPEKSLNNNEFQKILQSCLYNLPENTARVFTLKEILGFSSDEIQQ
                     MCGISTSNYHTIMHRARESLRQCLQIKWFNQENPK"
     gene            6112..6291
                     /locus_tag="WIC03_001164"
     CDS             6112..6291
                     /locus_tag="WIC03_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEZ3883667.1"
                     /translation="MKKCRDIALLLSKHQDRETTPGEKISIYTHLLFCPYCREYKRQL
                     QTIKRSLAKTTRTSK"
     gene            6532..6972
                     /locus_tag="WIC03_001165"
     CDS             6532..6972
                     /locus_tag="WIC03_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883668.1"
                     /translation="MIKETLMRPIFLSFVLLPILITACSTPDKSARWENIGTISNGNI
                     HTYINKDSVRKNGNLMIFQDKKVVTNLKQERFANTPAYKTAIAEWEIHCNNKTYRLSS
                     LQLFDTKNTEISTQSYTASSLRPMSILSGTLTEKQYETVCGKKL"
BASE COUNT         1827 a         1904 c         1636 g         1797 t
ORIGIN      
        1 ggtttttgtt aatccactat aaaaacacaa cctaaataaa aatgccgtct gaaccatatt
       61 tcaggtttca gacggcattt gcgtgtcgga tgcacaccgg acgggcggta agccgggttc
      121 tgtctcggac agtcattcct ctaggcatac cgttaccggt atgctcaagc aacctacccg
      181 aacgctcggc gggcagcgtc attgcgttct gtttggtctt gctccgaatg gggtttggcc
      241 tgccgcatat tgttaccaaa tgcgcggtgc gcccttaccg caccttttca cccttgcctg
      301 tgctgccaaa gcagccatcg gcggttttgc tttctgttcc actttccgtc gcgttaccgc
      361 gcccggccgt taaccggcat tctaccctgc ggagcccgga ctttcctccc cgtatgcctt
      421 acgcgatacg cggcgactgt ctgcccgtcc cgtgtgcggc gcggattata acacgaaacg
      481 caaaaatgcc gtctgaaacg gtacaggttt cagacggcat acggttcaaa ctacacgccc
      541 tgtttcaggc tggcttcgat aaagccgtcc aaatcgccat ccaatacggc tttggtgttg
      601 ccgacttcgt agcctgtacg caagtctttg atacgtgagg aatccaaaac atacgaacgg
      661 atttggctgc cccaacctac atcggattta ccttcttcca acgcctgttt ctcttcgttg
      721 cgtttgcgca tttccaattc atacagtttg gatttcaaca tttccatcgc ggccgctttg
      781 ttggcgtgtt gcgaacggtc gttttgacat tgcaccacaa tccccgtcgg ctcgtgggta
      841 atacgcacgg cggagtcggt tttgttgatg tgctgaccgc ccgcgcccga tgcgcgatag
      901 gtgtcgatac gcaaatcggc ggggttgatt tcgatttcaa tggaatcatc gatttcaggg
      961 taaacgaaca cggaggcaaa cgaagtatgg cgtttgttgt tcgagtcaaa cggtgaatag
     1021 cgcaccaagc ggtgaacgcc ggtttcggta cgcagcaaac cataggcgta ttcgccttcc
     1081 actcggatag tcgcgcggtt gatgcccgcg atttcgccgt cgtcttcttc gaggatttcg
     1141 attttgaagc ctttgcgctc ggcgtagcgg ctgtacatac ggaacagcat acccgcccag
     1201 tcttccgctt ccgtaccgcc cgcgcctgcg gtgatgtcga taaagcagtt gttcgggtcg
     1261 gcgggctggt tgaacatccg tttgaactcc aaatccgcca tctgtttttc cagccccgcc
     1321 acatcttcct gcacggcggc aaaaccttct tcgtcgtttt cttcgacggt catttcaatc
     1381 agcatgcggt tgtcttcgat gcccgtcgcg atgttgtcga gcgtcaacac gatgccttcg
     1441 aggattttgc gctctttgcc gatttcttgg gcgcgtttcg ggtcgttcca aagttcgggg
     1501 tcttcggaaa gaccgacaac ttcttccaat cggtctttct tgccctgata atccatataa
     1561 acgcggatgt cttcgctgcg cttttccaaa tcattcaggg tattgttgag ctggttgatt
     1621 acttcggctt ccatgattct tttgttcttt caaaatttta ggggcgtatt gtacgggatt
     1681 cgggtatttt tttctatgga taaagccttc tggaaacacg ttcggacggc atagcgtcaa
     1741 taacggtatg ccgccagttt gcgtttgatt tcaggcaatg cggcacgtgc tgcctcctca
     1801 cccaaccgga tggcgcgttt tttctgatcg aatccgccga ctgcacccaa atccaaaacc
     1861 tgcggtttga taaccacatc cgcctgcccc aactcatttt gcaacgcgga aacgctcatt
     1921 acgttcagcg tctgatcgaa ataagagaag aaaccttggc tgatgttttt gcccggacgg
     1981 gcggaaatat cgacggcaat cacgaaattc gccccctgcc gccgggcggc actgacgggc
     2041 acgggctgcg acagaccgcc gtcaacatat gtatgcctgc cgatgataac gggttggaac
     2101 acattgggaa tggcggcgga agcgcgcaca gcctgcccgg cattcccttg attgaaagcg
     2161 acggccttgc cggtttcaaa atcagtagca acagcggcaa atttgatggg aaactgctga
     2221 atccgcctgc cgccgacttt tcggttgatg taattttgca gcttttcgcc tttgataaaa
     2281 ccactggtgg acaaggttaa atcgactaaa tcggttttgc ctaaaatttc ggcttccaat
     2341 tcgaggcggt cgggcgacat acccgatgca aaaaggctgc cgactatcga acctgccgat
     2401 gtgccggtaa ccaccttcac aggaatacca ttttctttca aaaccttaat aatgcctaca
     2461 tgggcaaatc ctttagatgc gccgccaccg agtgccaaac cgaccactgc ggcgggtttg
     2521 gcggtttgca ccggcttgcg ggcagcatta tttcccgccg tgccgcaggc ggcaagcaac
     2581 gcggcggcgg cgattgccaa aagcggtctg atttttgaaa acgttaccat attttccatt
     2641 cctttatata tcgcaccccg tcaaaaagag ggattgcttt tcttaacaac cccctttgac
     2701 agccaagcaa atgggggctt tgttaagtca tcatcaaaat taatatttct ttttttcctt
     2761 tacggaaatt atatttgaag gcatactatc caaggcggga attatctcac aacaccgccg
     2821 ttatccaaat atcccgcctt tttcccttcc tttccatcaa aatactttct ttttatattc
     2881 attaacttgt taaatcattg gctgccgggt gtcagttttt ccgacaaaat ccgtctaatg
     2941 gggtatcaac agaaccaaaa caggaacact tatgaaaatc ggaacaactt ggcagacggc
     3001 atccgctatg ctggttttgc gtctgtttgc cgcatatgaa tttttggaat cgggtttgca
     3061 aaaatggaac ggggagaatt ggttttctga aatcaacgat caattcccat tcccgttcaa
     3121 cttgctgccg gacgcgttaa actggaatct cgccatgtat gcggagcttt tgctgcccgt
     3181 actgttgctt ttgggtttgg caacgcgtct gtcggcatta gggctgatgg tcgttaccgc
     3241 cgtcgcttgg gctgcggttc acgccggttc gggttacaat gtctgcgaca acggttataa
     3301 aatggcttta atttatatcg tggtattaat cccgctgctt ttccagggtg cgggcggatg
     3361 gtcgctggat acgctgctga aaaaacggtt ttgcccccaa tgccgtctga agcaagattg
     3421 attcagtcgt ggaatctgac tttaaacatt ccaaccttat ctcgttaact tgatattctg
     3481 aaaaggaaat agaaatgaac aaaaacattg ctgccgcact cgccggtgct ttatccctgt
     3541 ctttggccgc cggtgcagtt gctgccaaca aaccggcaag caacgcaaca ggcgttcata
     3601 aatccgccca tggctcttgc ggcgcgtcca aatctgccga aggttcgtgc ggcgcggctg
     3661 gttctaaagc aggcgaaggc aaatgcggcg agggcaaatg cggtgcgacc gtaaaaaaaa
     3721 cccacaaaca caccaaagca tctaaagcca aggccaaatc tgccgaaggc aaatgcggcg
     3781 aaggcaaatg cggttctaaa taatcccacc ccttcaaacc aagccgcgtt tttcagtaaa
     3841 atgcggcttt tttaacggca aacaaagatt ttttaacaag cacatcattc ttttgtgcca
     3901 tccgaaccgg gtaaaaatat gattcaacac gcaggcttgg gctaccgccg cgacttggcg
     3961 gaagactttc tctcgctttc cgaaaacagc ccgatatgct ttatcgaagc cgcaccggaa
     4021 aactggctga aaatgggcgg ctgggcgcgc aaacagtttg accgtgtggc ggaacggctg
     4081 ccgctggcgt tgcacggatt gtctatgtcg ctgggcgggc aagcaccgct ggatactgat
     4141 ttgatagacg gcatcaaaga aatgatgcgc cgttacgatt gcacgttttt ctccgaccat
     4201 ttgagctact gccacgacgg cggtcatctt tacgatttgt tgccgctgcc cttcaccgag
     4261 gaaatggtgc atcatacggc gcggcgtatc cgtgaagtgc aagaccgttt gggctgccgc
     4321 atcgccgtgg aaaacacgtc ctactatctg cattccccgc ttgccgagat gaacgaggtc
     4381 gagttcctca acgccgtcgc acgtgaggcc gattgcggca ttcatctgga tgtgaacaat
     4441 atctacgtca acgccgtcaa tcacggcctg ttgtcgccgg aggctttttt gaaaaacgtt
     4501 gacgcagatc gcgtgtgcta tatccatatt gccggacatg acgtggaaac gccggaattg
     4561 ttgattgata cacatggcgc ggcagttttg ccgactgttt gggacttgct cgaacttgcc
     4621 tatgccaagc tgccgacgat tccgcccacc ctgttggaac gcgattttaa tttcccgcct
     4681 tttgccgaac tcgaagccga agtcgccaaa atcgccgatt atcaaacgcg tgccggaaag
     4741 gaatgccgcc gtgcagcctg aaacctccgc ccaataccag caccgtttcg cccaagccat
     4801 acgcgggggc gaagccgcag acggtctgcc gcaagaccga ctgaacgtct atatccgcct
     4861 gatacgcaac aatatccaca gctttatcga ccgctgctat accgaaacgc ggcaatactt
     4921 tgacagcgaa gaatggagcc gtctgaaaga aggtttcgtc cgcgacgcgc acgcccaaac
     4981 gccctatttt caagaaatcc ccggcgagtt cctacaatat tgccaaagcc tgccgctttc
     5041 agacggcatt ttggcactga tggattttga atatacccaa ttactggcag aagttgcaca
     5101 aattccggat attcccgaca ttcattattc aaatcacagc aaatacacgc cctcccctgc
     5161 ggcctttatc cggcaatatc gatatgatgt taccgatgat ttgcaggaag cggaaacagc
     5221 cttgttaata tggcgaaacg ccgaagatga tgtgatgtac caaacattgg acggcttcga
     5281 tatgatgctg ctggagataa tgggttcctc cgcgctttcg tttgacaccc tcgcccaaac
     5341 ccttgtcgaa tttatgcctg aggacgataa ttggaaaaat attttgcttg ggaaatggtc
     5401 aggctggact gaacaaagga ttatcatccc ctccttgtcc gccatatccg aaaatatgga
     5461 aggcaattcc ccgggccaaa accatctatc cgcataaaat taccttgttc ccgatactat
     5521 gccgctaccc gacctgaccg atgccgaatt aatagagtcg cgtaaactgc ttctgcattt
     5581 tgcgcggctt cagttgcccg accaccctga tttggctgaa gatttagtgc aggaaacatt
     5641 gctgtccgca tacagcgcag gcgacagttt tcaaggcagg gcacttgtca acagctggct
     5701 ttttgccata ttgaaaaaca aaattattga cgcattacgt caaatcggaa ggcagaggaa
     5761 agtctttacc acactggatg acgagctact ggatgaagca tttgaaagcc atttttccca
     5821 aaacgggcat tggacgcagg aagggcagcc gcaacattgg aacactccgg aaaaatcatt
     5881 aaacaacaac gaattccaaa aaattctgca aagctgccta tacaacctgc ctgaaaacac
     5941 cgcacgggta tttaccctga aggaaatact cggtttttca tccgacgaaa tacaacaaat
     6001 gtgcggtatc agcacgtcca actaccacac cattatgcac cgcgcccgag aatcattgcg
     6061 ccaatgcctg caaatcaaat ggttcaacca agaaaacccg aagtaaacgt tatgaaaaaa
     6121 tgccgcgata tcgccctgct tctttccaaa catcaggacc gggaaaccac cccgggcgag
     6181 aagatttcca tatacacaca cctgctgttc tgtccgtatt gccgtgaata taaaagacaa
     6241 cttcaaacca tcaaaagatc actggcaaaa acaaccagaa cttcaaaata aatgccgtct
     6301 gaaaaggctt cagacggcat aagctgacgg aaacaaatca aaccgattta ctgttatctg
     6361 cagttcatcc ataatacaca cttcaaaagc agcatatttc cccatacgga atgtataaat
     6421 acgcaaaata cgaaggctgc atcaatttgc catatttgct ttatttgcct tatttcacag
     6481 acgacactcc ccctcccgcc caacccgttc tttctgaatg agcagatttc aatgattaag
     6541 gaaaccctaa tgcgcccaat cttcctatct ttcgttttac tccctatttt gataaccgcc
     6601 tgcagcacac cggacaagtc tgcccgatgg gaaaatatcg gcacaatctc aaacggcaat
     6661 attcatacat atatcaacaa agacagcgtg agaaaaaacg gaaatctgat gattttccaa
     6721 gataaaaaag ttgtgaccaa tctaaaacaa gaacgttttg ccaacacccc cgcatacaag
     6781 actgccattg ccgagtggga aatccactgc aacaacaaaa cataccgctt aagttcgcta
     6841 cagttgtttg atacaaaaaa cacggaaatt tccacacaaa gctacacagc ctcttccctc
     6901 cgcccgatga gcatcctgtc cggaacatta actgaaaaac aatatgaaac cgtatgcggg
     6961 aaaaaactct gattacaact tatgcacaaa cttatccata aaccttatta taaaaatgcc
     7021 gtctgaaata ctgaaatatc agcatttcgg acggcatttt gccatttccc cgaaaagtta
     7081 cccccaaagt tatccacatt attttttaaa ccagcttcca tccgaaatat agtggattaa
     7141 caaaaatcaa gacaaggcga cgaa
//
LOCUS       DAWHSK010000091         7155 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.091, whole genome shotgun sequence.
ACCESSION   DAWHSK010000091 DAWHSK010000000
VERSION     DAWHSK010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7155
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.091"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(146..1411)
                     /gene="ftsY"
                     /locus_tag="WIC03_001166"
     CDS             complement(146..1411)
                     /gene="ftsY"
                     /locus_tag="WIC03_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEZ3883669.1"
                     /translation="MFSFFRRKKKQETPAPEEAQVQETAAKAESEVTQIVENIKEDAE
                     SLAESVKGQVESAVETVSGAVEQVKETVAEMLSEAEEAAEKAAEQVEAAKEAIAETVG
                     EAVGQVQEAVATTEEHKLGWAARLKQGLTKSRDKMAKSLAGVFGGGQIDEDLYEELET
                     VLITSDMGMEATEYLMKDVRDRVSLKGLKDGNELRGALKDALYDLIKPLEKPLVLPET
                     KEPFVIMLAGINGAGKTTSIGKLAKYFQAHGKSVLLAAGDTFRAAAREQLQAWGERNN
                     VTVISQTTGDSAAVCFDAVQAAKARGIDIVLADTAGRLPTQLHLMEEIKKVKRVLQKA
                     MPDAPHEIIVVLDANIGQNAVNQVKAFDDALGLTGLIVTKLDGTAKGGILAALASDRP
                     VPVRYIGVGEGIDDLRPFDARAFVDALLD"
     gene            1557..3125
                     /gene="msrAB"
                     /locus_tag="WIC03_001167"
     CDS             1557..3125
                     /gene="msrAB"
                     /locus_tag="WIC03_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional peptide-methionine (S)-S-oxide
                     reductase MsrA/peptide-methionine (R)-S-oxide reductase
                     MsrB"
                     /protein_id="HEZ3883670.1"
                     /translation="MKHRTFFSLCAKFGCLLALGACSPKIVDAGAATVPHTLSTLKTA
                     DNRPASVYLKKDKPTLIKFWASWCPLCLSELGQTEKWAQDAKFSSANLITVASPGFLH
                     EKKDGDFQKWYAGLNYPKLPVVTDNGGTIAQSLNISVYPSWALIGKDGDVQRIVKGSI
                     NEAQALALIRDPNADLGSLKHSFYKPDTQKKDSKIMNTRTIYLAGGCFWGLEAYFQRI
                     DGVVDAVSGYANGNTKNPSYEDVSYRHTGHAETVKVTYDADKLSLDDILQYFFRVVDP
                     TSLNKQGNDTGTQYRSGVYYTDPAEKAVIAAALKREQQKYQLPLVVENEPLKNFYDAE
                     EYHQDYLIKNPNGYCHIDIRKADEPLPGKTKTAPQGKGFDAATYKKPSDAELKRTLTE
                     EQYQVTQNSATEYAFSHEYDHLFKPGIYVDVVSGEPLFSSADKYDSGCGWPSFTRPID
                     AKSVTEHDDFSYNMRRTEVRSHAADSHLGHVFPDGPRDKGGLRYCINGASLKFIPLEQ
                     MDAAGYGALKSKVK"
     gene            complement(3227..3712)
                     /locus_tag="WIC03_001168"
     CDS             complement(3227..3712)
                     /locus_tag="WIC03_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEZ3883671.1"
                     /translation="MDALHTIARNLTKKRQTVSCAESCTGGLLAAAFTSVAGSSQWFD
                     QSFVTYSNKAKEDRLGVLPETLLEHGAVSRQTVYEMARGAKAVAQADYAVGISGIAGP
                     GGGSESKPVGTVWFGFAFPGGSCEAMRRFDGNRESVRAQAVAFALERLAGLIENGGDA
                     V"
     gene            complement(3738..4586)
                     /locus_tag="WIC03_001169"
     CDS             complement(3738..4586)
                     /locus_tag="WIC03_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEZ3883672.1"
                     /translation="MDFKQFDFLHLISVSGWEHLAEKAWAFGLNLAAALLIFLVGKWA
                     AKRIVAVMRAAMTRAQVDATLISFLCNVANIGLLILVIIAALGRLGVSTTSVTALIGG
                     AGLAVALSLKDQLSNFAAGALIILFRPFKVGDFIRVGGFEGYVREIKMVQTSLRTTDN
                     EEVVLPNSVVMGNSIVNRSTLPLCRAQVIVGVDYNCDLKVAKEAVLKAAVEHPLSVQN
                     EERQAAAYITALGDNAIEITLWAWANEADRWTLQCDLNEQVVENLRKVNINIPFPQRD
                     IHIINS"
     gene            complement(4680..5681)
                     /locus_tag="WIC03_001170"
     CDS             complement(4680..5681)
                     /locus_tag="WIC03_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ3883673.1"
                     /translation="MKRKIWLLPLLAVSAYLQAQTEVRLAVHKSFSLPKGLIARFERA
                     NDAKVSIIQAGGANEMLNKLILSRANPIADAVYGLDNANIGKAREMGILAAAQPGSAP
                     VAVGLPSALAVDYGYVSINYDKKWFEGKKLPLPQTLQDLTRPEYKNLLVVPSPATSSP
                     GLGFLMANISGLGEEGAFKWWAQMRQNGVKVAKGWSEAYYTDFSHNGGAYPLVVGYAA
                     SPAAEVYFSKGKYSEPPTGNLFLKGGVFRQVEGAAVLKGAKQPELAAKLVQWLQSREV
                     QQAVPSEMWVYPAVKNTRLPDVFRFAQAPTHTTAPAQRDIDANQRGWVSRWIRTVLK"
     gene            5740..6420
                     /locus_tag="WIC03_001171"
     CDS             5740..6420
                     /locus_tag="WIC03_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HEZ3883674.1"
                     /translation="MQENPTVWLFDLDNTLHDADAGIFTLINRAMTRYMARRLKLSES
                     AASDLRQEYWHRYGATLAGLQIHHPEIDIAEFLRESHPIDAILTKLHGMADTEHTLCR
                     LNGRKAVFSNGPSFYVRAVVGALGLENRFDALFGTDDFGLLYKPNPQAYLNVCRLLDV
                     PPECCIMVDDSADNLHQAKALGMKTVWFGAKSHALSFIDACARDMAQLARYAETLSEH
                     RQTHYNTL"
     gene            6446..6718
                     /locus_tag="WIC03_001172"
     CDS             6446..6718
                     /locus_tag="WIC03_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex protein"
                     /protein_id="HEZ3883675.1"
                     /translation="MRKTFLILMTTAALLSGCAWETYQDGNGKTAVRQKYPAGTPVYY
                     QDGSYSKNMNYNQYRPERHAVLPNQTGNNADEEHRQHWQKPKFQNR"
     gene            6773..>7155
                     /locus_tag="WIC03_001173"
     CDS             6773..>7155
                     /locus_tag="WIC03_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEZ3883676.1"
                     /translation="MPQNTLNIVILAAGKGTRMYSKMPKVLHEIGGETMLGRVIDTAA
                     ALNPQNICVVVGHGKEQVLDTVKRDVVWVEQTEQLGTGHAVKTALPHLAAEGRTLVLY
                     GDVPLIDVETLETLLEAAGNEVGLLT"
BASE COUNT         1622 a         2153 c         1813 g         1567 t
ORIGIN      
        1 ctgttgttct aaggtctaga ttcccgcctg cgcgggaatg acaggtcttt tataatcttt
       61 gaatattgct gttatcccaa ggtctggatt cccgcctgcg cgggaatgac ggcatcggtc
      121 tgctgttttc ggacggcatt tcggctcaat ccagcagtgc gtccacaaac gcgcgcgcgt
      181 caaacgggcg caaatcgtct atgccttcgc ccacgccgat gtagcggacg gggacggggc
      241 ggtcggaggc aagcgcggca aggatgccgc cttttgccgt gccgtctagc ttggtaacga
      301 ttaaaccggt cagccccaat gcgtcgtcaa aggctttgac ttggttgacg gcgttttgcc
      361 cgatgttggc atcaagcacg acgatgattt cgtgcggcgc gtcgggcatg gctttttgca
      421 acacgcgctt cacttttttg atttcttcca tcaaatgaag ctgcgtgggc aggcggccgg
      481 cggtgtcggc cagcacaatg tcgatgccgc gtgctttggc agcttggacg gcatcaaaac
      541 aaacggctgc ggaatcgccc gtggtttgcg aaatcacggt tacgttgttg cgctcgcccc
      601 aagcttgaag ctgctcacgc gcggcggcac ggaaagtatc gcctgccgcc agcaatacgg
      661 attttccatg cgcttggaaa tatttggcga gtttgccgat agacgtggtt ttgcccgcgc
      721 cgttgatacc tgcaagcata atcacgaaag gctctttggt ttcgggcaag accaaaggtt
      781 tctccagagg cttaatcagg tcgtacagcg catctttcag cgcgccgcgc aattcgttgc
      841 cgtctttcag ccctttgagg ctgacgcggt cgcgcacgtc tttcatcagg tattcggtgg
      901 cttccatgcc catatcgctg gtaatcagca cggtttccag ctcttcgtac aaatcttcgt
      961 cgatttgtcc gccgccgaac acgcccgcca gcgatttcgc cattttgtcg cgcgatttgg
     1021 tcaaaccttg tttcaaacgc gccgcccaac cgagcttgtg ttcttcagtt gtcgcaacgg
     1081 cttcttgaac ttgcccgaca gcctcgccga cggtttcggc aatggcttct tttgccgctt
     1141 cgacttgttc cgctgctttt tccgccgctt cctctgcttc agacagcatc tcggcaacgg
     1201 tttcctttac ctgttcaacc gcaccgctga cggtttcaac ggcagattcg acctgccctt
     1261 tgacgctttc tgctaaagat tcagcatctt ctttaatatt ttcaactatt tgagtaactt
     1321 cggattctgc ttttgctgca gtttcctgaa cttgagcctc ctcgggagcc ggcgtttcct
     1381 gttttttctt gcgacggaag aagctgaaca ttgaattttc cttttaattt tagaaacttg
     1441 aaatagggtg tattgtagcg tattttgcgc ggcaaggttg tctgaaaatc cgggctgtaa
     1501 ggtttcggca tctcaaacgt ctaatcatgc aaaccgtcca cacaggaaac attaaaatga
     1561 aacaccgtac tttcttttcc ctttgcgcca agttcggctg cctgcttgcg ctgggtgctt
     1621 gttcgcccaa aatcgtcgat gccggagccg cgaccgtgcc gcacacttta tccactttga
     1681 aaaccgcaga caaccgcccc gccagtgttt acttgaaaaa agacaaaccg acgctgatta
     1741 aattttgggc aagctggtgt cctttgtgtc tgtccgaatt gggacagacc gaaaaatggg
     1801 cgcaagatgc aaaattcagc tccgccaacc tgattaccgt cgcgtctccg ggctttttgc
     1861 acgagaaaaa agacggcgac ttccaaaaat ggtatgccgg tttgaattat cccaagctgc
     1921 ccgtcgtaac cgacaacggc ggcacgatcg cccaaagcct gaatatcagc gtttaccctt
     1981 cgtgggcgtt aatcggtaaa gacggcgacg tgcagcgcat cgtcaaaggc agcatcaacg
     2041 aagcgcaggc gttggcgtta atccgcgacc cgaatgccga tttgggcagc ttgaaacatt
     2101 cgttctacaa acccgacact cagaaaaagg attcaaaaat catgaacacg cgcaccattt
     2161 acctcgccgg cggctgcttc tggggcttgg aagcctattt ccaacgcatc gacggcgtgg
     2221 ttgacgcggt atccggctac gccaacggca acacgaaaaa tccgagctat gaagacgtgt
     2281 cctaccgcca tacgggccac gccgaaaccg ttaaagtgac ctacgatgcc gacaaactca
     2341 gcctagacga catcctgcaa tatttcttcc gcgtcgttga tccgaccagc ctcaacaaac
     2401 agggcaacga caccggtacg caataccgca gcggcgtgta ctacaccgac cccgccgaaa
     2461 aagccgtcat cgccgccgcc ctcaaacgcg agcagcaaaa ataccaactg cccctcgttg
     2521 ttgaaaacga gccgctgaaa aacttctacg atgccgagga ataccatcag gactacctga
     2581 ttaaaaaccc caacggctac tgccacatcg atatccgcaa agccgacgaa ccgctgccgg
     2641 gcaaaaccaa gaccgccccg caaggcaaag gcttcgacgc ggcaacgtat aaaaaaccga
     2701 gtgacgccga actcaaacgc accctgaccg aagagcaata ccaagttacc caaaacagcg
     2761 cgaccgaata tgccttcagc cacgaatacg accatttgtt caaacccggc atttatgtgg
     2821 acgttgtcag cggcgaacct ttgttcagct ccgccgacaa atatgattcc ggctgcggct
     2881 ggccgagctt cacgcgcccg attgatgcaa aatccgttac cgaacacgat gatttcagct
     2941 acaacatgcg ccgcaccgaa gtgcgcagcc acgccgccga ctcacacttg gggcacgtct
     3001 tccccgacgg cccgcgcgac aaaggcggac tgcgctactg catcaacggc gcgagcttga
     3061 aattcatccc gctggaacaa atggacgcgg caggctacgg cgcgttgaag agtaaagtga
     3121 aataagccgc accgccgcct aacccgacaa aataccgtct gaaacctgca acgtttcaga
     3181 cggtattttt tatccggcgg ggattttgtc cagacggaga ttttgtttag acggcatcgc
     3241 cgccgttttc aatcagcccc gccaaccgtt ccaacgcgaa ggcgaccgcc tgcgcgcgga
     3301 cggattcgcg gttgccgtca aaacggcgca ttgcttcgca acttccgccc ggaaaggcaa
     3361 acccgaacca aaccgtgccg acgggtttgc tttcgctgcc gccgcccgga ccggcgatgc
     3421 cggaaatacc gacggcgtaa tccgcctgcg ccacggcttt cgcgccgcgc gccatctcat
     3481 agacggtttg gcggctgacc gcgccgtgtt cgagcagggt ttcgggcaac acgcccaagc
     3541 ggtcttcttt ggctttgttg ctgtatgtta caaaactttg atcgaaccat tgcgaactgc
     3601 ctgcaacgct tgtgaatgcg gcggcaagca gcccccccgt gcaggattcg gcacagctta
     3661 cggtttgacg ttttttcgtc aggtttcggg cgatggtgtg cagcgcgtcc atttcccact
     3721 cccctttcag acggcgttta agaattgatg atgtgtatgt cgcgttgcgg gaacgggatg
     3781 ttgatattga ctttgcggag gttttcgacc acttgttcgt tcaaatcgca ttgcagcgtc
     3841 cagcggtctg cttcgtttgc ccaagcccat aatgtgattt cgatggcatt gtcgcccaag
     3901 gcggtgatgt aggcggcggc ctgccgctct tcgttttgaa cgctcaaggg gtgttcgacg
     3961 gcggctttca acaccgcctc tttcgccact ttcaaatcgc agttgtaatc gacgccgact
     4021 atcacttggg cgcggcaaag cggcagggtg gaacggttga cgatgctgtt gcccatcacc
     4081 acgctgttgg gcagcacgac ttcttcgttg tcggtcgtcc gcaaagaagt ctgcaccatt
     4141 ttaatctctc ggacatatcc ttcaaaaccg ccgacgcgga taaaatcgcc gactttgaac
     4201 gggcggaaca ggataatcag cgcaccggcg gcaaaattgg acagctggtc tttcaaggac
     4261 aacgccaccg ccaaacccgc gccgccgatt aaggcggtta cggatgttgt ggaaacgccc
     4321 aatctgccca atgcggcaat aatcaccaaa atcaataagc cgatattggc aacattacac
     4381 aaaaaactaa tcagcgtggc atcgacctgc gcgcgcgtca tcgccgccct catcacagcg
     4441 acaatgcgtt tcgccgccca ttttccgacc aaaaaaataa gcagcgcggc ggcaaggttc
     4501 agcccgaacg cccacgcctt ttcagccaga tgctcccaac cggaaacact gatcaggtgt
     4561 aaaaaatcaa attgtttgaa gtccattgtt ttcctcttga tcgaacatcc gccccgtgcg
     4621 gcgtaatcgg cacaggtgta aaaatgccgt atgaagccct gcggggcggt atgtttgttt
     4681 tatttcaaaa ccgttctaat ccaacgggaa acccatccgc gctggttcgc atcaatatcg
     4741 cgctgcgcgg gggcggtggt gtgcgtcggg gcttgggcga agcggaacac gtcgggcagg
     4801 cgcgtgtttt tgacggcggg gtaaacccac atttcggacg gaaccgcctg ctgcacttcc
     4861 cgactttgca gccattgcac cagttttgcc gccaattccg gctgtttcgc gcccttcaag
     4921 accgccgcgc cttcgacctg gcggaatacg ccgcctttta aaaacaggtt gcccgtcggc
     4981 ggctcgctgt atttgccttt ggaaaaatac acttccgccg ccgggctggc ggcataaccg
     5041 accaccagcg gatacgcgcc gccgttgtgc gaaaagtcgg tgtaatacgc ctcgctccag
     5101 cctttggcga ccttcacgcc gttctgccgc atctgtgccc accatttgaa cgcgccttct
     5161 tcgcccagac cgctgatgtt cgccatcagg aagcccagcc ccggggacga cgtggcggga
     5221 gacggcacga ccaataggtt tttatattcg gggcgggtca aatcctgcag ggtttgcggc
     5281 agaggaagct ttttgccttc aaaccatttt ttgtcgtaat tgatggacac atagccgtaa
     5341 tcgaccgcca aagccgaagg cagcccgacc gcgacggggg cggatccggg ttgcgccgcc
     5401 gccaaaatgc ccatttcccg cgccttgccg atattggcgt tgtccaagcc gtacaccgcg
     5461 tcggcgatcg ggttggcgcg gctcaaaatc agtttgttga gcatttcgtt cgcgccgccc
     5521 gcctgaataa tcgacacctt cgcatcgttt gcccgctcga agcgcgcaat cagccctttg
     5581 ggcaggctga acgacttatg caccgccagc ctgacttccg tctgcgcctg caggtatgcc
     5641 gaaaccgcca gcagcggcag cagccaaatt ttccgtttca ttccgagtcc tcctctattc
     5701 gctgtaaaat aacattctaa caaattttca cggttcaaaa tgcaagaaaa cccgaccgtg
     5761 tggctgttcg acctcgacaa cacgctgcac gatgccgacg caggcatctt cacactcatc
     5821 aaccgcgcta tgacacgcta tatggcacgc cgcctcaaac tctccgaatc cgccgcgtcc
     5881 gacctgcgtc aggaatattg gcaccgctac ggcgcaacgc tcgccggact gcaaatccac
     5941 catcccgaaa tcgacatcgc cgaattttta cgcgaaagcc atccgatcga tgcaatcctg
     6001 accaagctgc acggcatggc ggatacggaa cacaccctat gccgtctgaa cgggcgcaag
     6061 gcggtttttt ccaacggtcc gtcgttttac gtccgtgccg ttgtcggcgc actcggtttg
     6121 gaaaaccgtt tcgacgcact gttcggcacg gatgatttcg gactgctgta caaacccaat
     6181 ccgcaagcgt atctcaatgt ctgccgcctt ttggacgtac cgcccgaatg ctgcattatg
     6241 gtggacgaca gcgcggacaa cctgcatcag gcaaaagcat tgggcatgaa aaccgtctgg
     6301 ttcggtgcaa aatcccacgc gctgtccttt atcgacgcct gcgcacgcga tatggcgcaa
     6361 ctggctcggt atgcagaaac tttgtcagaa caccgccaaa ctcattacaa taccctctaa
     6421 atcctgaaaa tacgaaagaa aagccatgcg taaaaccttc cttatcctga tgaccaccgc
     6481 cgcccttttg tcgggctgcg cgtgggaaac ttatcaagac ggcaacggca agaccgccgt
     6541 ccgtcaaaaa tatcccgccg gcacgcccgt ttattaccaa gacggcagct actcgaaaaa
     6601 tatgaactac aaccaatacc gtcccgaacg ccatgccgtg ttacccaacc aaaccggcaa
     6661 caacgccgac gaagagcatc gccaacactg gcaaaagccc aaatttcaaa accgataaac
     6721 ccaccctatg ccgtctgaag ccctttcaga cggcattgca caggaaaacg ctatgccgca
     6781 aaacacttta aacatcgtca tcctcgccgc cggcaaaggc acgcgcatgt attccaaaat
     6841 gcccaaggtt ttgcacgaaa tcggcggcga aacaatgctc gggcgcgtta tcgacaccgc
     6901 cgccgcgctg aatccgcaaa acatctgcgt cgtcgtcggc cacggcaaag agcaagtttt
     6961 agacaccgtc aaacgcgatg tcgtctgggt tgaacaaacc gaacagctcg gcaccggcca
     7021 cgccgtcaaa accgccctgc cccaccttgc cgccgaaggc cgcacgctgg tgttgtacgg
     7081 cgacgttcct ttaattgacg ttgaaaccct cgaaaccctg ctcgaagccg caggcaacga
     7141 agtcgggctg ttgac
//
LOCUS       DAWHSK010000092         7150 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.092, whole genome shotgun sequence.
ACCESSION   DAWHSK010000092 DAWHSK010000000
VERSION     DAWHSK010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7150
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.092"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            152..1090
                     /locus_tag="WIC03_001174"
     CDS             152..1090
                     /locus_tag="WIC03_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEZ3883677.1"
                     /translation="MSIHTLKRLPSSLLLGLCLSLPSAHLFADNDILGQFLEQNMLTS
                     SDPIEIFAESTIHPTNTQAITGGLILSSQSALVVNNKTGQILYQKNADRIMPIASISK
                     LMSAMVVLDANLDMNETVTITPDEIDRIKGTGSRLAIGTALTRKELLHLSLMSSENRA
                     THALGRTYPGGMGAFVAAMNRKAQSLGMYGSRFYEPTGLNFQNVSTAKDLSLMVNAAA
                     QYPQIRTNSTSNYASVQTKNGQQNYKNSNALVREGMWNIELQKTGYIREAGRSMVVKA
                     NIQNQPVTIVLLNSPTSATRVNDARKIESWMLQQRS"
     gene            1219..1512
                     /gene="hfq"
                     /locus_tag="WIC03_001175"
     CDS             1219..1512
                     /gene="hfq"
                     /locus_tag="WIC03_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEZ3883678.1"
                     /translation="MTAKGQMLQDPFLNALRKEHVPVSIYLVNGIKLQGQVESFDQYV
                     VLLRNTSVTQMVYKHAISTIVPARSVNLQHENRPQAAPTSTLVQVETVQQPAE"
     gene            1597..2163
                     /locus_tag="WIC03_001176"
     CDS             1597..2163
                     /locus_tag="WIC03_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ3883679.1"
                     /translation="MLLKNILPFAHCLLRQALPEGGNALDGTAGNGHDTLFLAQTAGI
                     RGKVWAFDIQPQALSRTRCRLKEARYENVQLILDGHENLKQYVSEPLNAAIFNFGWLP
                     GGDKSLTTHTETSIPALCATLSLLKEGGMLIAVLYPEHENGKQEAEAIEQWAKNLPQE
                     QFAVLRYGFTNRKNSPPYLLAFEKLRQK"
     gene            2217..2591
                     /locus_tag="WIC03_001177"
     CDS             2217..2591
                     /locus_tag="WIC03_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HEZ3883680.1"
                     /translation="MQYLIVKYSHQIFVTITILVFNIRFFLLWKNPEKPLAGFWKALP
                     HLNDTMLLFTGLWLMKITHFSPFNAPWLGTKILLLLAYIALGMMMMRARPRSTKFYTV
                     YLLAMCCVACIVYLAKTKVLPF"
     gene            2601..3095
                     /gene="folK"
                     /locus_tag="WIC03_001178"
     CDS             2601..3095
                     /gene="folK"
                     /locus_tag="WIC03_001178"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEZ3883681.1"
                     /translation="MNNRHFAVIALGSNLENPAQQVRAALDTLSSHPDIRLKQASSLY
                     MTAPVGYDNQPDFVNAVCTVSTTLDGIALLAELNRIEADFGRERSFRNAPRTLDLDII
                     DFDGISSDDPRLTLPHPRAHERSFVIRPLAEILPDFILGKHGKVAELSKRLGNQGIRL
                     LPDK"
     gene            complement(3222..3701)
                     /locus_tag="WIC03_001179"
     CDS             complement(3222..3701)
                     /locus_tag="WIC03_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEZ3883682.1"
                     /translation="MPESIFKQISLDILKLHRDSVYSLLATSGCNCQVHEAAYVNIDG
                     KYYISLSCEPEVGEVKTGILLIEDESRNLRLSWVGSARELDRKDNAYKRALSALSRKL
                     GRCKDRLHTAVQPFLLELVPEKGRFSVGDEEVWISRNDLVRALYPVGYSMRQAMLQI"
     gene            complement(3921..4658)
                     /gene="ubiE"
                     /locus_tag="WIC03_001180"
     CDS             complement(3921..4658)
                     /gene="ubiE"
                     /locus_tag="WIC03_001180"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HEZ3883683.1"
                     /translation="MGGQKTHFGFSTVNEDEKAGKVAEVFHSVAKNYDIMNDVMSAGL
                     HRVWKHFTINTAHLKKGDKVLDIAGGTGDLSRGWAKRVGKEGEVWLTDINSSMLTVGR
                     DRLLNEGMILPVSLADAEKLPFPDNYFNLVSVAFGLRNMTHKDAALKEMYRVLKPGGT
                     LLVLEFSKIYKPLEGAYDFYSFKLLPVMGRLIAKDAESYQYLAESIRMHPDQETLKQM
                     MLDAGFDSVDYHNMSAGIVALHKGVKF"
     gene            complement(4701..5069)
                     /locus_tag="WIC03_001181"
     CDS             complement(4701..5069)
                     /locus_tag="WIC03_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HEZ3883684.1"
                     /translation="MNNIPVEIRLLKNRTVLVLTYGDEPKNLPAEFLRVYSPSAEVRG
                     HGVGQDVLQTGKADVQITDLQPVGQYALKISFSDGHDSGLYDWAYLHRLAYGYDAMWQ
                     EYLDKLAAAGASRFEKQSDC"
     gene            5189..6808
                     /gene="mnmC"
                     /locus_tag="WIC03_001182"
     CDS             5189..6808
                     /gene="mnmC"
                     /locus_tag="WIC03_001182"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HEZ3883685.1"
                     /translation="MDNLVWDGIPDIRTLDQTIRKHAHPLNLIVCLPDNQIPDFQTAQ
                     DASDAECRLKHRLDQATQCLQFDSINLIEHILPDVRFWLVPPSRTRRLHEHFHHISWQ
                     TEAIPQTESKPDKPWFALPQTSERQKPEHILVIGAGISGAATAHALASYGISVTVLEA
                     RKAAQAASGNRQGLLYAKISPHDTEQTELLLAGYGYTKRLLGHILPESETWGGNGIIH
                     LNYSRTEQQRNHELGLQKHHNHLYRSITSAEAEKIAGIPLSVPYDHPSCGLYWQHGVW
                     LNPPAFVRTLLNHPLIGLHEDTPLTDISHDGEKWIASTPNGIFSATHIIYCTGAHSPR
                     LPETNLAALPLRQIRGQTGLTPSTPFSEQLRCAVSGESYISPSWHGLHCYGASFIPNS
                     SHTGWNEAEEASNRQALAHLNPALSESLFAANPNPQKHQGHAAIRCDSPDHLPLVGAL
                     GDIAAMRQTYTKLALDKNYRIDTPCPYLPNAYVNTAHGTRGLATAPICAAAIAAQILG
                     LPHPLSKRLRHALHPNRTIIRAIVRRKDLTP"
BASE COUNT         1912 a         2227 c         1433 g         1578 t
ORIGIN      
        1 ttccgattta aaaacagaat gttataaaac ccaatcccca aaacacaaca agaccgcccg
       61 cctacgggca gtctcctgtc agacgacata ctttacagat ggctgttttt tcaacaaaat
      121 aacgccaata ctcaaaaata tggaatcaaa aatgtccatc catactctga aacgcctgcc
      181 ctcatcgctg ctgctcggtc tctgcctttc cctgccgtca gcccaccttt ttgccgacaa
      241 cgacatttta gggcaatttt tagaacagaa catgcttacc tcctccgatc cgatagaaat
      301 attcgccgaa agcacgatac accccaccaa cacccaagcc attacaggcg gtctgattct
      361 ctcctcacaa tctgccctgg tcgtcaacaa caaaaccgga cagatactgt atcagaaaaa
      421 cgccgacagg attatgccca tcgcctccat ttccaaactg atgagcgcga tggtcgtttt
      481 ggatgcaaac ttggacatga acgaaaccgt taccattacg cccgacgaaa tcgaccgcat
      541 caaagggacc ggcagccgtc ttgccatagg tacggcactt acacgcaaag aactgctgca
      601 cctgagcctg atgagcagcg aaaaccgcgc cacccatgca ttgggcagaa cctaccccgg
      661 cggcatgggc gcatttgtcg ccgccatgaa ccgcaaagcc caaagcctcg gtatgtacgg
      721 cagccgcttt tacgaaccga ccggactcaa cttccaaaac gtttctaccg ccaaagactt
      781 gagccttatg gtcaacgccg ccgcccaata tccgcaaatc cgcaccaact cgacttccaa
      841 ctacgcctcg gtacagacca aaaacgggca gcagaactac aaaaactcca atgccctggt
      901 cagagaaggc atgtggaaca tcgaattgca gaaaaccggc tacatacgcg aagcaggcag
      961 gtctatggtt gtcaaagcca acattcaaaa tcaacccgtt accatcgtat tgctgaactc
     1021 gcccacatcc gccacacgcg tcaacgacgc ccgcaaaatc gaatcgtgga tgctgcagca
     1081 acgctcctga catacaaatg cccggcggaa aaccgcccaa acacaaaaaa aattggtaat
     1141 ttttcctatt caaggctaca attcgacacg cacagggcat ctgcccgatt tcacaatcat
     1201 aatagcggag ttaaaaatat gacagctaaa ggacaaatgt tgcaagatcc cttcctgaac
     1261 gccctgcgta aagagcatgt tccggtttcg atttacttag ttaacggtat caaattacaa
     1321 ggtcaggttg agtctttcga tcaatacgtt gttctcctga gaaacacttc cgtcacccaa
     1381 atggtttaca aacacgccat ttccaccatc gtaccggcac gctccgtcaa cctacaacat
     1441 gaaaacagac cccaagccgc accgacttcg accctcgtcc aagtggaaac cgtccagcag
     1501 cctgccgaat aatccgcacg aagcatgacg tgtcatatct ttcaatacct taccggacaa
     1561 cggtaaggta tttttatttt cagacggcat ttgaaaatgt tattgaaaaa catccttcca
     1621 ttcgcccatt gccttttgcg gcaggcactt cccgaaggcg gcaatgcgct ggacggcacc
     1681 gccggcaacg gacacgacac ccttttcctc gcacaaaccg caggcatccg ggggaaagtg
     1741 tgggcattcg acatccagcc gcaagccctg agccgcactc gatgccgtct gaaagaagcg
     1801 agatacgaga atgtgcaact catcttagac ggacacgaaa acctgaaaca atatgtttca
     1861 gaaccactaa atgccgccat tttcaacttt ggctggctgc ccggcggaga taaaagcctt
     1921 accacccata cggaaaccag cattcccgcc ctttgcgcaa ccctctccct gctgaaagaa
     1981 ggcggtatgc ttattgccgt cctctatccg gaacacgaaa acggcaaaca ggaggcagaa
     2041 gcaatcgaac aatgggcaaa aaacctgcct caagaacagt ttgccgtttt gcgttacggc
     2101 tttaccaacc ggaaaaacag cccgccctat cttttggcat ttgaaaaact gcgtcaaaaa
     2161 taactgtttg cggtaaaata agccgattct aacccttatt ttgatacgag ccgaccatgc
     2221 agtatctgat tgtcaaatac agccatcaaa tcttcgttac catcaccatt ttggtattca
     2281 acatccgttt tttcctactt tggaaaaatc cagaaaagcc cttggcgggc ttttggaaag
     2341 cactgcccca ccttaacgac accatgctgc tgtttacggg attgtggctg atgaaaatta
     2401 cccatttctc cccgttcaac gcgccttggc tcggtacaaa aatcctgctt ctgctcgcct
     2461 atatcgcatt gggtatgatg atgatgcgcg cccgtccgcg ttcaaccaag ttctacaccg
     2521 tttacctgct cgctatgtgt tgcgtcgcct gcatcgttta ccttgccaaa accaaagtcc
     2581 tgcctttctg aaacaccgtt atgaacaaca gacattttgc cgtcatcgcc ctgggcagta
     2641 atcttgaaaa ccctgcccaa caggtacgcg ccgcattgga cacgctgtcg tcccatcctg
     2701 acatccgtct taaacaggct tcctcactgt atatgaccgc gcccgtcggt tacgacaatc
     2761 agcctgactt tgtcaatgcc gtctgcaccg tttccaccac cttggacggc attgccctgc
     2821 ttgccgaact caaccgtatc gaagctgatt tcggacgcga acgcagcttc cgcaacgcgc
     2881 cgcgcacatt ggatttggac attatcgact ttgacggcat ctccagcgac gacccccgcc
     2941 ttaccctgcc acatccgcgc gcgcacgaac gcagtttcgt catacgccct ttggcagaaa
     3001 tcctccctga ttttattttg ggaaaacacg gaaaggttgc cgaattgtca aaacggctgg
     3061 gcaatcaagg catccgtctt ttaccggata agtaattccg cacgcggatg ccgtctgaat
     3121 gccggtttca gacggcattt tttctttgcc gccaacacgc gtgcaaaaaa atcgcccctt
     3181 ggaaaagggg cgcaaaagga acacaaacca ctaccaaaac tttaaatctg caacattgcc
     3241 tgccgcatac tgtatccgac aggatataaa gccctcacta aatcgtttcg agaaatccaa
     3301 acttcttcat cgccgacaga aaatctgcct ttctccggca ccagctccaa caaaaacggt
     3361 tgaaccgccg tatgcagcct gtccttacac cgccccagct ttctggacaa ggcggacagg
     3421 gcgcgtttgt aggcattatc cttgcggtca agttcccgcg cactgccgac ccagctcaaa
     3481 cgaaggttgc ggctttcatc ctcaatcagc aaaatgcctg ttttgacttc ccccacctct
     3541 ggctcgcatg aaagcgaaat ataatatttg ccatcgatgt tgacatacgc cgcctcatgc
     3601 acctgacagt tgcagccgga agtggcaagc agcgaataaa cagaatcccg atgcagtttc
     3661 aaaatatcaa gggaaatctg tttaaaaata ctttccggca tttttccctc ctttcgtctg
     3721 ctgcgttgtt tactggattg actgcattat acagacatct cccgatcttg caaatgataa
     3781 ttgttcctat tttttaaaaa atttataaat tattgataga tatgtgaatt aattttaaga
     3841 tttaaaatct ctttctcaaa aaaacacaaa aatgccgtct gaaacgtgtt cagacggcat
     3901 tggctgcaca ggcagtctgt ttaaaatttt acgcccttat gtagcgcgac gatgcccgca
     3961 ctcatattgt gataatccac gctgtcaaag cccgcatcca acatcatctg tttcaaagtt
     4021 tcctgatcgg ggtgcatacg gatggattcg gcaagatatt gataactctc cgcatctttg
     4081 gcaatcagcc tgcccatgac cggcagcagc ttgaacgaat agaaatcata cgcgccttcc
     4141 aaaggtttgt agattttgga aaactccaac accagcaacg tgccgcccgg tttcaaaaca
     4201 cggtacatct ctttcagcgc ggcatcttta tgcgtcatgt tccgcaagcc gaacgccacg
     4261 gaaaccaagt tgaaataatt gtcggggaaa ggcagttttt ccgcatcggc aagcgatacg
     4321 ggcaaaatca tgccttcgtt caacagacgg tcgcgcccga cggtcagcat agaggaatta
     4381 atatcggtca gccaaacctc gccttccttg ccgacccgtt tcgcccaacc gcgcgacaaa
     4441 tcgcccgtac cgcccgcaat gtccaacact ttatcgcctt ttttcaggtg cgccgtgttg
     4501 atggtgaaat gcttccacac cctgtgcagc cctgccgaca tcacatcgtt cataatgtcg
     4561 tagtttttgg cgacggagtg gaacacttcc gccacttttc cggctttctc atcttcgttg
     4621 accgtactga atccgaaatg cgttttttgt ccgcccatct tctgaccacc tccctgatgc
     4681 cgtcctccgg catcccaaac ttaacaatct gattgctttt caaaacgcga cgcaccagcc
     4741 gccgccaatt tgtccaaata ttcctgccac atcgcatcgt atccgtatgc cagtctgtgc
     4801 agatacgccc aatcgtaaag accgctgtcg tgcccgtccg aaaaactgat tttcagcgca
     4861 tactgtccga caggctgcaa atccgtgatt tggacatccg ccttgccggt ctgcaaaaca
     4921 tcctgtccca cgccgtgtcc gcgcacttcc gcactcggcg aatagacgcg taaaaattcg
     4981 gcaggcagat ttttaggttc gtccccataa gtcaaaacca acacggtacg gtttttcaac
     5041 aaacggattt caacaggaat attattcaaa aaacacctcg gttcggcata caatatccgg
     5101 caggcgcagg cgtgccttca aacggcgcgt attttaacag ctttatcccg ataccgatat
     5161 tcaaacccac atttcagacg gcattgatat ggacaatctc gtatgggacg gcattcccga
     5221 catccgcaca ctcgaccaaa ccatccgcaa acacgcacac ccgctcaacc tgattgtctg
     5281 cctccccgat aatcagattc ccgattttca aaccgcacaa gatgcttcgg acgcggaatg
     5341 ccgtctgaag caccgtttgg atcaggcaac gcagtgcctc cagttcgaca gcatcaacct
     5401 catcgaacac atcctgcccg atgtccgctt ctggctggtt cccccttcac gcacccgccg
     5461 cctgcacgaa cacttccacc acatttcctg gcagaccgaa gccatcccgc aaaccgaaag
     5521 taagcccgac aaaccctggt ttgcacttcc acaaacatcc gaacggcaaa aaccggaaca
     5581 catcctcgtt atcggagcgg gcatatccgg cgcggcaacc gcccacgcct tagcatcata
     5641 cggcatttcc gttaccgtat tggaagcccg aaaagccgcc caagccgcca gcggcaaccg
     5701 ccaagggctg ctctacgcca aaatctcgcc gcacgacacc gaacaaaccg aactgctgct
     5761 tgccggctac ggctacacca aacgcctgct cggacatatc ctgcccgaat ccgaaacctg
     5821 gggcggcaac ggcatcatcc acctcaatta cagccgcacc gaacaacaac gcaatcacga
     5881 attgggtttg caaaaacacc ataaccacct ctaccgcagc atcacatctg cagaagccga
     5941 aaaaatcgcc ggtatcccac tgtccgtccc atacgaccac ccttcatgcg gactctactg
     6001 gcaacacggc gtatggctca atccacccgc attcgtccgc accctcctca accatccgct
     6061 cattggacta cacgaagaca caccgttaac cgacatttcc cacgacgggg aaaagtggat
     6121 tgcaagcacg ccaaacggca tattttccgc cacacacatc atctactgca ccggcgcgca
     6181 cagcccccgc ctgcccgaaa ccaacctcgc cgccctgcct ctcaggcaaa tacgcggaca
     6241 aaccggcctc acaccgtcca ccccgttttc cgaacaactg cgttgcgccg tttcaggcga
     6301 aagctacatc agcccgtcgt ggcacggact gcactgctac ggcgcgagtt ttattcccaa
     6361 cagcagccat accggatgga acgaagccga agaagcctca aaccgccaag cattggcaca
     6421 ccttaacccc gccctttccg aatcattgtt tgccgccaac ccaaaccccc aaaaacacca
     6481 agggcacgcc gccatacgct gcgacagccc cgaccacctt cccctagtcg gcgcactcgg
     6541 cgacattgcc gccatgcggc agacctacac caaactcgcg ctggacaaaa actaccgcat
     6601 cgacacccca tgcccatacc tgcctaatgc ctacgtcaac accgcgcacg gcacccgcgg
     6661 actcgccacc gcccccatct gcgccgccgc cattgcagcc caaatcctag gcttgcccca
     6721 tcccctctca aaacgcctgc gccacgccct acaccccaac cgcaccatca tccgcgccat
     6781 cgtcagaagg aaggatctaa ccccttaagc ctttgccgca tgccgtcccg accacagaaa
     6841 atcatattca aataaacata ccaatcctga cggtaaaata tcgaaaacac ccataacgtt
     6901 ttcttcccaa aataaggagt aaggaatgcc taacaaattg cctttgctgt ctaagatgaa
     6961 tgttccgtaa cattcgaaga cttccttaac cctgccagcc gtttcggttt ctgtaccccc
     7021 tgttagataa ggaaggaatg aaaaagccct gttgattgta tgaagtaaag ggcgttaatt
     7081 tttgttaatc ttcccttctc aggaactcaa tatataagga tgttaatttt tatttccctt
     7141 ctcgaggact
//
LOCUS       DAWHSK010000093         7140 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.093, whole genome shotgun sequence.
ACCESSION   DAWHSK010000093 DAWHSK010000000
VERSION     DAWHSK010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7140
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.093"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..497
                     /locus_tag="WIC03_001183"
     CDS             <1..497
                     /locus_tag="WIC03_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfite reductase subunit alpha"
                     /protein_id="HEZ3883686.1"
                     /translation="RVFVERNDGFRLPEDSRKPIVMIGSGTGVAPFRAFVQQRAAENA
                     EGKNWLIFGNPHFAADFLYQTEWQQFAKDGFLHRYDFAWSRDQEEKIYVQDKIREQAE
                     GLWQWLQEGAHIYVCGDAAKMAKDVEAALLDVIIGAGHLDEEGAEEYLDMLREEKRYQ
                     RDVY"
     gene            524..2293
                     /gene="cysI"
                     /locus_tag="WIC03_001184"
     CDS             524..2293
                     /gene="cysI"
                     /locus_tag="WIC03_001184"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HEZ3883687.1"
                     /translation="MTVQTKTKGLAWQEKPLSDNERLKTESNFLRGTILDDLKDPLTG
                     GFKGDNFQLIRFHGMYEQDDRDIRAERAEAKLEPLKFMLLRCRLPGGIIKPSQWIELD
                     KFARENSHYRSIRLTNRQTFQFHGVPKAKLQTMHRLLHKLGLDSIATAADMNRNVLCT
                     SNPIESELHRQAYEYAKKISEHLLPRTRGYLDVWVDGKKVQSSDDFLQEDEPILGKTY
                     LPRKFKTAVVIPPLNDVDCYGNDLDFVAISDGGGQLAGFNVLAGGGLSMEHGNTKTYP
                     NISLELGFVPPEHALKAAEAVVTTQRDFGNRSDRKNARTRYTIQNMGLDNFRAEVERR
                     MGMPFEPIRPFKFTGRGDRIGWVKGIDGNWHLTLFIESGRLVDEGGKQLLTGVLEIAK
                     IHKGDFRITANQNLIVANVAEADKAKIEELARTYGLIRNDVSKLRENAMSCVSFPTCP
                     LAMAEAERVLPDFIDELDKIMAKHGTSDDYIVTRITGCPNGCGRAMLAEIGLVGKAVE
                     RYNLHIGGDREGVRIPRLYKENITLPEILAELDDLIGKWAAERNIGEGFGDFAIRTGI
                     VKPVLNAPVDFWDVSKAVAIARA"
     gene            2466..4325
                     /gene="ilvD"
                     /locus_tag="WIC03_001185"
     CDS             2466..4325
                     /gene="ilvD"
                     /locus_tag="WIC03_001185"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEZ3883688.1"
                     /translation="MPEYRSKTSTHGRNMAGARALWRATGVMETDFGKPIIAVANSFT
                     QFVPGHVHLHNMGQLVAREIEKAGAIAKEFNTIAIDDGIAMGHSGMLYSLPSRDLIAD
                     SIEYMVNAHCADALVCISNCDKITPGMLIAAMRLNIPTIFVSGGPMEAGKVIGVANIQ
                     PERRLDLIDAMIESADDNVSNRQVEEVEQNACPTCGSCSGMFTANSMNCLTEALGLSL
                     PGNGSYLATHAGRKELFLEAGRMIVEITKRYYEQNDETVLPRSIATKKAFENAMTMDI
                     AMGGSTNTILHLLAVANEAGVDFKMADIDRLSRVVPCICKTAPNNHDYYMEDVHRAGG
                     IFAILKELDKAGKLHTDVHTIHAPTLKDAIEKWDVTNPENTRAIERFKAAPGGVRTTQ
                     AFSQNRMWKTLDLDREKGCIRDVAHAYSQDGGLAVLFGNIAERGCVVKTAGVDESILK
                     FTGRARVFESQEAAVEGILGNQIVAGDIVIIRYEGPKGGPGMQEMLYPTSYLKSKGLG
                     KACALLTDGRFSGGTSGLSIGHASPEAAEGGAIGLVHEGDTVEIDIPNRSIHLVISDE
                     ELAARRAEMEARGSKAWKPENRDRYVSAALRAYGAMATSADKGAVRDVAQIER"
     gene            complement(4388..4648)
                     /locus_tag="WIC03_001186"
     CDS             complement(4388..4648)
                     /locus_tag="WIC03_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEZ3883689.1"
                     /translation="MTKAVRRKNVQTPKICYAFRTPSAYGQYLADAGFDYLSFANNHS
                     NDFGAQGITATAAGGSSFTYSIALKPLPITRPKLRKIPPSPR"
     gene            4971..5606
                     /locus_tag="WIC03_001187"
     CDS             4971..5606
                     /locus_tag="WIC03_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEZ3883690.1"
                     /translation="MNIKYSGIENRFETAILKKNGVRYGFVSFAPNLAAVKLNDYAKV
                     RKLITKTKQKADIVIVMFHGGAEGKQAEHLPFDTEIFYGENRGNVVEFARLAVDSGAD
                     VVFGQGPHVTRAVELYRDRFISYSGGNFATYGAINTSGISGIAPIFKIITDKQGRFVS
                     GNIIPITQVGDKIPKIDPEKTVIGRIIYLNRSDFPKGNGLDVSPDGSITRR"
     gene            complement(5684..6736)
                     /gene="bioB"
                     /locus_tag="WIC03_001188"
     CDS             complement(5684..6736)
                     /gene="bioB"
                     /locus_tag="WIC03_001188"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEZ3883691.1"
                     /translation="MTVSPVALRRKTECKPHPTARYWKKCDVEALFGLPFLDLIYQAA
                     EIHRQNFNPREIQLSTLLSIKTGGCPEDCAYCPQSAHHNTNLGKEQMMDVDEIVEKAK
                     IAKSRGASRFCMGAAWRGPKPKDVETVSAIIKAVKGLGMETCGTFGMLEEGMAEDLKE
                     AGLDYYNHNLDTDPDRYNDIIHTRQHEDRMDTLGKVRNAGLKVCCGGIVGMNETRAER
                     AGLIASLANLDPQPESVPINRLVKVEGTPLADAEDLDWTEFVRTIAVARITMPQSYVR
                     LSAGRSNMPEAMQAMCFMAGANSIFYGDKLLTTGNPDEDGDRILMEKLNLYPLQFEPE
                     GEVAEVEKASGIKVDY"
     gene            complement(6943..>7140)
                     /locus_tag="WIC03_001189"
     CDS             complement(6943..>7140)
                     /locus_tag="WIC03_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225218.1"
                     /note="ligates
                     L-alanyl-gamma-D-glutamyl-meso-diaminopimelate to
                     UDP-N-acetylmuramic acid for reincorporation into
                     peptidoglycan; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HEZ3883692.1"
                     /translation="FCYAGGVDWNVAEALAPLGGRLHVGKDFDAFVAEIVKNAEAGDH
                     ILVMSNGGFGGIHGKLLDALR"
BASE COUNT         1699 a         2051 c         1931 g         1459 t
ORIGIN      
        1 tgcgcgtgtt tgtggaacgc aacgacggct tcaggctgcc cgaagacagc cgcaagccga
       61 ttgtgatgat cggctcgggc accggcgtcg caccgttccg cgctttcgtc caacaacgtg
      121 ccgcagaaaa tgcggaaggc aaaaactggc tgattttcgg caatccgcat tttgccgccg
      181 acttcctcta tcagaccgaa tggcagcagt ttgccaaaga cggcttcctg cacagatacg
      241 atttcgcctg gtcgcgcgat caggaagaaa aaatctatgt gcaggacaaa atccgcgaac
      301 aggcggaagg actttggcaa tggctgcagg aaggcgcgca tatctatgtg tgcggcgatg
      361 cggcaaaaat ggcaaaagac gtggaagccg ccttgctgga tgtgattatc ggggcaggac
      421 atttggacga agagggcgca gaagaatatt tggatatgct gcgcgaagaa aaacgctatc
      481 agcgtgatgt ttattgatta aatataatcg ggaggaacac aaaatgaccg tacagaccaa
      541 gacaaaaggt ttggcgtggc aagaaaaacc gctatccgac aacgaacgtc tgaaaaccga
      601 aagcaatttt ttacgcggca cgattttgga cgatttgaaa gacccgctca cgggcggctt
      661 caaaggcgac aacttccaac tcatccgctt ccacggtatg tatgagcagg acgaccgcga
      721 catccgcgcc gaacgcgccg aggcaaaact cgagcctttg aaatttatgc ttttgcgctg
      781 ccgactgccg ggcgggatca tcaaaccgtc ccaatggata gaactggaca aatttgcccg
      841 ggaaaacagt cattaccgct ccatccggct gaccaaccgg caaaccttcc aatttcacgg
      901 cgtgccgaaa gccaagttgc agacgatgca ccgcctcttg cacaaactgg gtttggattc
      961 catcgccacg gcggcggata tgaaccgcaa cgtgctttgc acgtccaacc cgatcgagtc
     1021 cgaactgcac cggcaggctt acgaatacgc gaaaaagatt tccgaacacc tgctgccgcg
     1081 cacgcgcggt tatctggatg tgtgggtgga cggcaaaaaa gttcaaagtt ccgacgactt
     1141 ccttcaagaa gacgagccga ttttgggcaa aacctatctg ccgcgcaaat tcaaaaccgc
     1201 agtcgtcatc ccgcccttaa atgatgtgga ctgctacggc aacgacttgg attttgtcgc
     1261 catttcagac ggcggcggac agcttgccgg ttttaatgtt ttggcaggcg gcgggctttc
     1321 aatggaacac ggcaacacca aaacctatcc gaacatttca ctggaactgg gtttcgtacc
     1381 tccggaacac gcgctgaagg ccgccgaagc ggtggtaacc acacagcgcg acttcggcaa
     1441 ccgcagcgac cgcaaaaacg cccgcacccg ctacaccatt caaaatatgg gcttggacaa
     1501 cttccgcgcc gaagtcgaac gccgtatggg tatgccgttc gaacccatac gcccgttcaa
     1561 atttaccggg cgcggcgacc gcatcggctg ggtaaaaggc atagacggca actggcattt
     1621 gacccttttc atcgaaagcg gccgtttggt tgacgaaggc gggaaacagc ttctgaccgg
     1681 cgtgttggaa atcgccaaaa tccacaaagg cgatttccgc attaccgcca accaaaacct
     1741 catcgtggca aatgtcgccg aagcagacaa agcaaaaatc gaagaacttg cccgaacata
     1801 cggattaatc cgcaacgatg tcagcaaact gcgcgaaaat gcgatgtcgt gcgtttcctt
     1861 cccgacctgc ccgctggcaa tggcggaagc cgaacgcgtg ctgccggact tcatcgacga
     1921 gctggataag attatggcga aacacggcac gtcggacgac tacatcgtta cccgcattac
     1981 cggctgcccc aacggctgcg gacgggcaat gttggcggaa atcgggctgg tcggcaaagc
     2041 cgtcgaacgc tacaacctcc atatcggcgg cgaccgtgaa ggcgtacgca tcccccgcct
     2101 ttataaagaa aacatcaccc tgcccgaaat ccttgccgaa ttggacgacc tgatcggcaa
     2161 atgggcggca gaacgcaata tcggcgaagg tttcggcgat ttcgccatac ggacgggcat
     2221 cgtcaaaccc gtattaaatg cacccgttga tttttgggac gtatccaaag ccgtcgcgat
     2281 tgcccgcgcc tgaacccgtc cccgcattcc tgcggggatt ttcttttttg ccgcacgccg
     2341 tttcaatccc gcaaaattcc gatacttggt ataaaatacc ctcttttccc actctaaaaa
     2401 accgtacccg ataccgtctg aaacagccct gtctttcaga cggtataggc acaaggaaac
     2461 acactatgcc agaataccgc tccaaaacct ccacccacgg ccgcaatatg gcgggcgcac
     2521 gtgcattgtg gcgcgccacc ggcgtgatgg aaaccgactt cggcaagccc atcatcgccg
     2581 ttgccaactc cttcacccaa ttcgtgcccg gccatgtcca cctgcacaat atgggccagc
     2641 tggttgcccg cgaaatcgaa aaagccggcg ccatcgccaa agaattcaac accatcgcca
     2701 tcgacgacgg catcgctatg ggacacagcg gcatgctgta ctccctgccc agccgcgatt
     2761 tgattgccga ctccatcgaa tatatggtca atgcccactg cgccgacgcg ctggtgtgca
     2821 tttccaactg cgacaaaatc accccgggca tgctgattgc cgcgatgcgc ctgaacatcc
     2881 cgaccatctt cgtttccggc ggtccgatgg aagcaggtaa agttatcggc gtggcaaaca
     2941 tccagcccga acgccgtttg gacttgattg acgcgatgat tgaatcggcg gacgacaatg
     3001 tcagcaaccg gcaagttgaa gaagtcgaac aaaacgcctg cccgacctgc ggctcgtgtt
     3061 cgggtatgtt tacggcaaac tcgatgaact gcctgaccga agcactcggt ctgtccctac
     3121 ccggcaacgg ttcgtatttg gcgacccacg ccggccgcaa agaattgttc ctcgaagccg
     3181 gccgtatgat tgtcgaaatc accaaacgct attacgagca aaacgatgaa accgtgttac
     3241 cgcgcagcat tgccaccaaa aaagcgtttg aaaacgctat gacgatggat attgcgatgg
     3301 gcggcagcac caacactatt ttgcatttac tcgccgttgc caacgaagcg ggcgtggatt
     3361 tcaaaatggc agacatcgac cgcttaagcc gcgttgtgcc ctgcatctgc aaaaccgcac
     3421 ccaacaacca cgactactat atggaagacg tgcaccgagc cggcggcatc ttcgccatcc
     3481 tgaaagaatt ggacaaagcg ggcaagctgc acaccgacgt gcacactatc cacgcaccga
     3541 cgctgaaaga cgcgattgaa aaatgggacg taaccaaccc tgaaaacacc cgcgccatcg
     3601 aacgcttcaa agccgcgccc ggcggcgtac gcaccaccca agcgttctcg caaaaccgta
     3661 tgtggaaaac actcgacctc gaccgcgaaa aaggctgtat ccgcgacgtg gcacacgcct
     3721 actcgcaaga cggcggtttg gcggtcttgt tcggcaacat cgccgaacgc ggctgcgtgg
     3781 taaaaaccgc aggcgtggac gagagcatcc tcaaattcac cggccgcgcg cgcgtgtttg
     3841 aaagccagga agccgctgtc gaaggcattt tgggcaacca aatcgtcgcc ggcgacatcg
     3901 tcatcatccg ctacgaaggc ccgaaaggcg gcccgggcat gcaggaaatg ctgtatccga
     3961 cttcctacct gaaatctaaa ggcctcggca aagcctgcgc tctcttaacc gacggacgct
     4021 tctccggcgg cacatcaggt ttgtccatcg gacacgcctc gcccgaagcg gcggaaggtg
     4081 gcgcgatcgg tttggtacac gaaggcgata ccgtcgaaat cgacatcccc aaccgcagca
     4141 tccaccttgt catttccgat gaagagcttg ccgcgcgccg tgccgaaatg gaagcacgcg
     4201 gcagcaaagc gtggaaaccg gaaaaccgcg accgctacgt ctccgccgca ttaagggctt
     4261 acggcgcgat ggcgacttcc gccgacaaag gcgcggtgcg cgacgtagcg caaatcgaaa
     4321 gataagcccc aaaacgtgcg gcaaatgctg tctcgtctga acagtaaagg tctgatgatg
     4381 gacaaattta tctgggcgat ggcggtattt tccgcaattt tggcctcgtt atcggcagcg
     4441 gctttagcgc gattgagtat gtaaaagagc tgccgcccgc cgccgttgcc gtgatgcctt
     4501 gcgcgccgaa gtcgttgctg tggttgttgg cgaagctgag gtagtcgaat cccgcgtcgg
     4561 caaggtattg cccgtatgcg gagggcgttc ggaatgcata gcatattttg ggggtttgca
     4621 cattttttcg gcgtaccgcc ttcgtcaaac agcgtgcctt cgaggttgcc gacggtaatg
     4681 tccgcgtctt gcaaggcaga ttcgacgttt ttcagaatat tggtatcggg caggtaatcg
     4741 accggataat tgctgccgag cataatgtcg cccacgccga taatggaaac ggtatcggct
     4801 gtcttttcgt caggcgcatc ctgtccgtcc acatcgccgg tatttgaaac gggcgcgggg
     4861 tttgaaccgt caatcggcag cacggggatt ccgttccggc aggatgcgcc gagggcttga
     4921 ccccggcggt ttgcggtgcg gaaacggcat aaagggcgaa ccttgacgaa ttgaacatca
     4981 aatattcggg catcgaaaac aggtttgaaa ccgccatcct gaagaaaaac ggcgtaagat
     5041 acggcttcgt ctccttcgcc cccaaccttg ccgccgtcaa actgaacgat tatgccaaag
     5101 ttagaaaact gattaccaaa accaaacaga aagccgacat cgtcattgta atgttccacg
     5161 gcggcgcgga agggaaacag gcggaacacc tgccgttcga taccgaaatc ttctatgggg
     5221 aaaacagggg caacgtcgtc gagtttgcac ggcttgccgt cgattccggc gcggatgtcg
     5281 tattcgggca ggggccgcac gttactcgcg ccgtcgaact ttaccgcgac cgcttcatct
     5341 cctacagcgg cggcaacttt gccacctacg gcgcaatcaa cacctccggc atcagcggca
     5401 tcgcgccgat tttcaaaatt atcaccgaca aacaggggcg gttcgtttcc ggtaacatta
     5461 tccccatcac tcaagtcggc gataaaatcc ccaaaatcga ccccgagaaa accgttatcg
     5521 ggcggattat ttatctgaac cgcagcgatt tccccaaggg gaacggactg gatgtctcgc
     5581 ccgacggcag catcacgcgc cggtaaaaag atattggaaa tgccgtccgg acaaatgccg
     5641 tctgaaagcc tttttccggg tttcagacgg catttttcaa tcgtcaataa tccactttaa
     5701 tcccagaggc tttttccact tcggcgacct cgccttccgg ttcaaactgc aagggataca
     5761 ggttgagctt ttccatcagg attctgtcgc cgtcctcatc aggattgccc gtggtcagca
     5821 gcttgtcgcc gtaaaaaatc gagttcgcgc ccgccataaa gcacatcgcc tgcattgctt
     5881 caggcatatt gctgcgccct gccgacagcc ggacataact ttgcggcatc gtaatccgcg
     5941 ccacggcgat ggtgcggaca aattccgtcc agtccaaatc ttcggcatcg gcaagcggcg
     6001 tgccttccac tttgaccaac cggttaatcg gcacgctttc gggctgcggg tcgagattgg
     6061 cgagactggc aatcagcccg gcacgttcgg cgcgggtttc gttcatcccg acgatgccgc
     6121 cgcagcagac tttcaaaccg gcgttgcgga ctttgcccaa ggtgtccatt cggtcttcgt
     6181 gttggcgggt gtggatgatg tcgttgtagc ggtcggggtc ggtgtcgagg ttgtggttgt
     6241 aataatccaa gcccgcctct ttcaagtctt ccgccatacc ttcttcgagc ataccgaacg
     6301 tgccgcaggt ttccataccc aagcccttga cggctttgat gattgcggaa accgtctcca
     6361 cgtctttggg tttagggccg cgccacgccg cgcccataca aaaccggctt gcgccgcgcg
     6421 atttggcgat tttggctttt tcgacgattt catccacatc catcatctgc tctttgccca
     6481 gattggtatt gtggtgcgcc gattgcggac aataggcgca gtcttcggga caaccgccgg
     6541 ttttgatgga caacagcgtg gaaagctgga tttcgcgcgg gttgaaattt tggcggtgga
     6601 tttcggcggc ttggtaaatg aggtcgagga agggaagtcc gaacagggct tcgacatcgc
     6661 attttttcca atagcgcgcg gtgggatgag gcttgcactc ggtcttacgg cgcaaggcga
     6721 cgggggatac ggtcataatg tgttctttcg tatttacagc ggcgcaatga cgctgacagg
     6781 caaaccgccg gcgcaatgac ggcggttgcg ggtaatcaaa tggctgaaaa cagcggcggc
     6841 aagtgtaacg cgtttgaaaa tacggggcaa acggtttttc cgcctttgtg ccgcgcagcc
     6901 ggacgatgcc gtctgaaggg ctttcagacg gcatcgcccg ggctatctca aagcgtccag
     6961 cagctttccg tgtattccgc cgaaaccgcc gttgctcatc accaaaatat ggtcgcctgc
     7021 ttcggcgttt ttcacgattt cggcaacgaa ggcatcgaag tctttgccga cgtgcagcct
     7081 gccgcccaaa ggcgcgaggg cttcggcgac gttccagtcc acgccaccgg cgtagcagaa
//
LOCUS       DAWHSK010000094         7067 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.094, whole genome shotgun sequence.
ACCESSION   DAWHSK010000094 DAWHSK010000000
VERSION     DAWHSK010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7067
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.094"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(79..1425)
                     /gene="mnmE"
                     /locus_tag="WIC03_001190"
     CDS             complement(79..1425)
                     /gene="mnmE"
                     /locus_tag="WIC03_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEZ3883693.1"
                     /translation="MSDNVPTIAAVATAPGRGGVGVIRISGKNLLPMAQALCGKTPKP
                     RVATYADFTDADGQAIDSGLLLFFAAPASFTGEDVIELQGHGGPVVMDMLLNRCLELG
                     ARLAEPGEFTKRAFLNDKLDLAQAEGVADLIDASSRSAARLALRSLKGDFSRRIHGLV
                     EDLITLRMLVEATLDFPEEDIDFLEAADARGKLDGLRRAVDDVLANAQQGAILREGLN
                     VVLVGAPNVGKSSLLNALAGDEVAIVTDIAGTTRDAVRERILIDGVPVHIVDTAGLRE
                     TDDVVERIGIERSRKAVSEADVALVLVDPREGLNEKTRAILDALPSELKRIEIHSKSD
                     LHAHAAGGFGTGAETVIALSAKTGDGLDALKRTLLREAGWQGEGEGLFLARTRHVNAL
                     KAAQEELSLAALCGNHQIELFAEHLRLAQVACGEITGEFTADDLLGVIFSRFCIGK"
     gene            1556..1711
                     /locus_tag="WIC03_001191"
     CDS             1556..1711
                     /locus_tag="WIC03_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883694.1"
                     /translation="MEKRNTYAFLLGIGSLLGLFHPAKIAIRPNPADDLKNIGGDFQR
                     AIEKARK"
     gene            1708..2139
                     /locus_tag="WIC03_001192"
     CDS             1708..2139
                     /locus_tag="WIC03_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2335 domain-containing protein"
                     /protein_id="HEZ3883695.1"
                     /translation="MTENAQDKARQAVETVVKSPELVEQILSDEYVQIMIARRFHSGP
                     LPPPSDLAQYNDIISNGADRIMAMAEKEQAVRHETIRQDQTFNRRGQLYGFISVILIL
                     LFAVFLVWSGYPATAASLAGGTVVALAGAFVIGRSRDQGKN"
     gene            2273..6664
                     /gene="app"
                     /locus_tag="WIC03_001193"
     CDS             2273..6664
                     /gene="app"
                     /locus_tag="WIC03_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_168458961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesion and penetration autotransporter App"
                     /protein_id="HEZ3883696.1"
                     /translation="MKTTDKRTTETHRKAPKTGRIRFSPAYLAICLSFGILPQAWAGH
                     TYFGINYQYYRDFAENKGKFAVGAKDIEVYNKKGELVGKSMTKAPMIDFSVVSRNGVA
                     ALVGDQYIVSVAHNGGYNNVDFGAEGSNPDQHRFSYQIVKRNNYKAGTKGHPYGGDYH
                     MPRLHKFVTDAEPVEMTSYMDGWKYTDQNNYPDRVRIGAGRQYWRSDEDEPNNRESSY
                     HIASAYSWLVGGNTFAQNGSGGGTVNLGSEKIKHSPYGFLPTGGSFGDSGSPMFIYDA
                     QKQKWLINGVLQTGNPYIGKSNGFQLVRKNWFYDEIFTGDTHSVFYEPHQNGKYTFHD
                     NNNGTGKINAKHEHNSLPNRLKTRTVQLFNVSLSETAREPVYHAAGGVNSYRPRLNNG
                     ENISFIDEGKGELILTSNINQGAGGLYFQGDFTVSPENNETWQGAGVHISEDSTVTWK
                     VNGVANDRLSKIGKGTLHVQAKGENQGSISVGDGTVILDQQADDKGKKQAFSEIGLVS
                     GRGTVQLNADNQFNPDKLYFGFRGGRLDLNRHSLSFHRIQNTDEGAMIVNHNATTTST
                     VTITGNESITQPSGKNINRLNYSKEIAYNGWFGEKDATKTNGRLNLVYQPAAEDRTLL
                     LSGGTNLNGNITQTNGKLFFSGRPTPHAYNHLNNHWSQKEGIPHGEIVWDNDWINRTF
                     KAENFHIQGGQAVISRNVAKVEGDWHLSNHAQAVFGVAPHQSHTICTRSDWTGLTNCV
                     ENTITDDKVIASLTNTDISGNVDLADHAHLNLTGLATLNGNLTAGGDTHYEVTHNATQ
                     RGNLSLVGNAQATFNQATLNGNTSASGNASFNLSNNAVQNGSLTLSGNAKANVSHSAL
                     NGNVSLADKAVFHFENSRLTGQISGGKDTALHLKDSEWTLPSGTELGNLNLDNATITL
                     NSAYRHDAAGAQTGSVSDTPRRRSRRSRRSLLSVTPPTSAESRFNTLTVNGKLNGQGT
                     FRFMSELFGYRSDKLKLAESSEGTYTLAVNNTGNEPASLEQLTVVEGKDNKPLSENLN
                     FTLQNEHVDAGAWRYQLIRKDGEFRLHNPVKEQELSDKLGKAEAKKQAGKDNAQSLDA
                     LIAAGRDAVEKTESVAEPARQAGGENVGIMQAEEEKKRVQADKDSALAKQREAETRPA
                     TTAFPRARRARRDLPQPQPQPQPQPQPQPQRDLISRYANSGLSEFSATLNSVFAVQDE
                     LDRVFAEDRRNAVWTSGIRDTKHYRSQDFRAYRQQTDLRQIGMQKNLGSGRVGILFSH
                     NRTENTFDDGIGNSARLAHGAVFGQYGIDRFYIGISAGAGFSSGSLSDGIGGKIRRRV
                     LHYGIQARYRAGFGGFGIEPHIGATRYFVQKADYRYENVNIATPGLSFNRYRAGIKAD
                     YSFKPAQHISITPYLSLSYTDAASGKVRTRVNTAVLAQDFGKTRSAEWGVNAEIKGFM
                     LSLHAAAAKGPQLEAQHSAGIKLGYRW"
BASE COUNT         1883 a         2043 c         1709 g         1432 t
ORIGIN      
        1 aaccaaacgg atcggattcc cgtttttatg ggataacgga atgttcagcc ggacaccgcc
       61 acgatgcttt cgatccgttt attttccgat acaaaacctc gaaaaaatca cgccgagcag
      121 gtcgtccgcc gtaaactcgc ccgtgatttc gccgcatgcg acctgcgcca agcgcaagtg
      181 ttcggcaaac agctcgattt gatggttgcc gcacaatgcc gccagcgaca attcttcctg
      241 cgctgctttg agtgcgttga cgtgccgcgt ccgcgccaaa aacagccctt cgccctcgcc
      301 ctgccatccg gcctcgcgca acaacgtccg tttcagcgcg tccaagccgt cgccagtttt
      361 cgccgacagc gcgatgacgg tttccgcgcc cgtaccgaac ccgcctgccg cgtgtgcatg
      421 caaatcagat ttgctgtgga tttcgatgcg tttcaactcc gacggcagcg cgtccaaaat
      481 cgcccgtgtc ttttcattca aaccctcgcg cggatcgacc aacaccagcg cgacatcggc
      541 ttcggatacg gctttgcggc tgcgttcgat gccgatacgc tcgaccacgt cgtccgtctc
      601 gcgcaaacct gccgtatcga caatatgcac cggcacgccg tcaatcagga tacgttccct
      661 gactgcgtcg cgcgtcgttc cggcaatatc ggtaacaatc gccacttcgt cgcccgccag
      721 agcattcagc aggctcgact tgcccacatt cggcgcaccg accaatacga cattcagacc
      781 ttcgcgcaaa atcgcgccct gctgtgcgtt ggcaagcaca tcatccacgg cgcggcgcaa
      841 gccgtccagt ttgccgcgtg cgtccgccgc ttcgagaaaa tcaatgtctt cctcgggaaa
      901 atccagcgtc gcttcgacca gcatccgcaa ggtaatcaaa tcttcgacca gaccgtgtat
      961 ccgccgcgaa aaatcgccct tgagcgagcg caacgccaga cgcgccgccg aacggctgga
     1021 tgcgtcaatc aaatccgcca caccttccgc ctgtgccaag tccagtttgt cgttcaaaaa
     1081 cgcacgtttg gtaaactcgc ccggttcggc aaggcgcgcg cccagttcca aacagcggtt
     1141 cagcagcata tccatcacca ccggcccgcc gtgtccctga agctcgatga catcttcgcc
     1201 cgtaaaactt gccggtgcgg caaaaaacag tagcagcccg ctgtcgattg cctgtccgtc
     1261 cgcgtccgta aaatcagcat aggttgccac gcgcggcttg ggcgttttcc cgcacaaagc
     1321 ctgcgccatc ggcagcaggt ttttccccga tatgcgtatc acgcccacgc cgccgcgccc
     1381 tggtgcggta gcgactgccg caatcgttgg aacgttatcc gacataaaac ccccgaaaat
     1441 tcaaaacagc tgcgattata gcaaatgccg tctgaagtcc gacggtttgg ctttcagacg
     1501 gcataaaacc gcaaaaatgc ttgataaatc cgtccgcctg acctaatata accatatgga
     1561 aaaacgaaac acatacgcct tcctgctcgg tataggttcg ctgttgggcc tgttccatcc
     1621 cgcaaaaatc gccatccgcc ccaatcccgc cgacgatctc aaaaacatcg gcggcgattt
     1681 tcaacgcgcc atagagaaag cgcgcaaatg accgaaaacg cacaggacaa ggcgcggcag
     1741 gctgtcgaaa ccgtcgtcaa atccccggag cttgtcgagc aaatcctgtc cgacgagtac
     1801 gtgcaaataa tgatagctcg gcgtttccat tcgggaccgt tgccgccgcc gtccgacttg
     1861 gcgcaataca acgacattat cagcaacggg gcagaccgca ttatggcaat ggcggaaaaa
     1921 gaacaagccg tccggcacga aaccatacgg caagaccaaa ccttcaacag gcgcgggcaa
     1981 ctgtacggct tcatcagcgt catcctgata ctgctttttg ccgtcttcct cgtatggagc
     2041 ggctaccccg caaccgccgc ctcccttgcc ggcggcacag tggttgcctt ggcgggtgct
     2101 ttcgtgattg gaagaagccg agaccaaggc aaaaattaat gacaaatcct agggcgtgct
     2161 tcatatccgc ccgaacgccg aaccgcacat ataggcacat cccgcgcgcc gcctggaagc
     2221 ggaagccgcg ccctcccaaa caaacccgaa tcccgtcaga taaggaaaaa taatgaaaac
     2281 aaccgacaaa cggacaaccg aaacacaccg caaagccccg aaaaccggcc gcatccgctt
     2341 ctcgcctgct tacttagcca tatgcctgtc gttcggcatt cttccccaag cctgggcggg
     2401 acacacttat ttcggcatca actaccaata ctatcgcgac tttgccgaaa ataaaggcaa
     2461 gtttgcagtc ggggcgaaag atattgaggt ttacaacaaa aaaggggagt tggtcggcaa
     2521 atcaatgaca aaagccccga tgattgattt ttctgtggtg tcgcgtaacg gcgtggcggc
     2581 attggtgggc gatcaatata ttgtgagcgt ggcacataac ggcggttata acaatgttga
     2641 ttttggtgcg gagggaagca atcccgatca gcaccgtttt tcttaccaaa ttgtgaaaag
     2701 aaataattat aaagcaggga ctaaaggcca tccttatggc ggcgattatc atatgccgcg
     2761 tttgcataaa tttgtcacag atgcagaacc tgttgagatg accagttata tggatgggtg
     2821 gaaatatacc gatcaaaata attatcctga ccgtgttcga atcggagcag gcagacaata
     2881 ttggcggtct gatgaagatg aacccaataa ccgcgaaagt tcatatcata ttgcaagtgc
     2941 gtattcttgg ctcgttggtg gcaatacctt tgcacaaaat ggatcaggtg gtggcacagt
     3001 caacttaggt agtgaaaaaa ttaaacatag cccatatggt tttttaccaa caggaggctc
     3061 atttggcgac agtggctcac caatgtttat ctatgatgcc caaaagcaaa agtggttaat
     3121 taatggggta ttgcaaacag gcaaccccta tataggaaaa agcaatggct tccagctagt
     3181 gcgtaaaaat tggttttatg acgaaatttt taccggagat acccattcag tattctacga
     3241 accacatcaa aatgggaaat acacttttca cgacaataat aatggcacag gaaaaatcaa
     3301 tgccaaacat gaacacaatt ctctgcctaa tagattaaaa acacgaaccg ttcaattgtt
     3361 taatgtttct ttatccgaga cagcaagaga acctgtttat catgctgcag gtggtgtcaa
     3421 cagttatcga cccagactga ataatggaga aaatatttcc tttattgacg aagggaaagg
     3481 cgaattgata cttaccagta acatcaacca aggtgctgga ggattatatt tccaaggaga
     3541 ttttacggtc tcgcctgaaa acaacgaaac gtggcaaggt gcgggcgttc atatcagtga
     3601 agacagtacc gttacttgga aagtaaacgg cgtggcaaac gaccgcctgt ccaaaatcgg
     3661 caaaggcacg ctgcacgttc aagccaaagg ggaaaaccaa ggctcgatca gcgtgggcga
     3721 cggtacagtc attttggatc agcaggcaga cgataaaggc aaaaaacaag cctttagtga
     3781 aatcggcttg gtcagcggca ggggtacggt gcaactgaat gccgataatc agttcaaccc
     3841 cgacaaactc tatttcggct ttcgcggcgg acgtttggat ttaaacaggc attcgctttc
     3901 gttccaccgt attcaaaata ccgatgaagg ggcgatgatt gtcaatcata atgccacaac
     3961 aacatccacc gttaccatta cagggaatga aagtattaca caaccgagtg gtaagaatat
     4021 caatagactt aattacagca aagaaattgc ctacaacggt tggtttggcg agaaagatgc
     4081 gaccaaaacg aatgggcggc tcaaccttgt ttaccagccc gccgcagaag accgcaccct
     4141 gctgctttcc ggcggaacaa atttaaacgg taacatcacg caaacaaacg gcaaactgtt
     4201 tttcagcggc agaccaacac cgcacgccta caatcattta aacaaccatt ggtcgcaaaa
     4261 agagggcatt ccgcacggag aaatcgtgtg ggacaacgac tggattaacc gcacgtttaa
     4321 agcggaaaat ttccatattc agggcgggca ggcggtgatt tcccgcaatg ttgccaaagt
     4381 ggaaggcgat tggcatttga gcaatcacgc ccaagcagtt ttcggtgtcg caccgcatca
     4441 aagccacaca atctgtacac gttcggactg gacgggtctg acaaattgtg tcgaaaacac
     4501 cattaccgac gataaagtga ttgcttcatt gaccaatacc gacatcagcg gcaatgtcga
     4561 tcttgccgat cacgctcatt taaatctcac agggcttgcc acgctcaacg gcaatctgac
     4621 cgcaggcgga gacacgcact atgaggttac gcacaacgcc acccaaagag gcaaccttag
     4681 cctcgtgggc aatgcccaag caacatttaa tcaagccaca ttaaacggaa acacatcggc
     4741 ttcgggcaat gcttcattta atctaagcaa caacgccgta caaaacggca gtctgacgct
     4801 ttccggcaac gctaaggcaa acgtaagcca ttccgcactc aacggcaatg tctccctagc
     4861 cgataaggca gtattccatt ttgaaaacag ccgccttacc ggacaaatca gcggcggcaa
     4921 ggatacggca ttacacttaa aagacagcga atggacgctg ccgtcaggca cggaattagg
     4981 caatttaaac cttgacaacg ccaccattac actcaattcc gcctatcgcc acgatgctgc
     5041 aggcgcgcaa accggcagtg tgtcagacac gccgcgccgc cgttcgcgcc gttcgcgccg
     5101 ttccctatta tccgttacac cgccaacttc ggcagaatcc cgtttcaaca cgctgacggt
     5161 aaacggcaaa ttgaacggtc agggaacatt ccgctttatg tcggaactct tcggctaccg
     5221 cagcgacaaa ttgaagctgg cggaaagttc cgaaggcact tacaccttgg cggtcaacaa
     5281 taccggcaac gaacctgcaa gcctcgaaca attgacggta gtggaaggaa aagacaacaa
     5341 accgctgtcc gaaaacctta atttcaccct gcaaaacgaa cacgtcgatg ccggcgcgtg
     5401 gcgttaccaa ctcatccgca aagacggcga gttccgcctg cataatccgg tcaaagaaca
     5461 agagctttcc gacaaactcg gcaaggcaga agccaaaaaa caggcgggaa aagacaacgc
     5521 gcaaagcctt gacgcgctga ttgcggccgg gcgcgatgcc gtcgaaaaga cagaaagcgt
     5581 tgccgaaccg gcccggcagg caggcgggga aaatgtcggc attatgcagg cggaggaaga
     5641 gaaaaaacgg gtgcaggcgg ataaagacag cgccttggcg aaacagcgcg aagcggaaac
     5701 ccggccggct accaccgcct tcccccgcgc ccgccgcgcc cgccgggatt tgccgcaacc
     5761 gcaaccccaa ccgcaacccc aaccgcagcc ccaaccgcag cgcgacctga tcagccgtta
     5821 tgccaatagc ggtttgagtg aattttccgc cacgctcaac agcgttttcg ccgtacaaga
     5881 cgaattagac cgcgtatttg ccgaagaccg ccgcaacgcc gtttggacaa gcggcatccg
     5941 ggacaccaaa cactaccgtt cgcaagattt ccgcgcctac cgccaacaaa ccgacctgcg
     6001 ccaaatcggt atgcagaaaa acctcggcag cgggcgcgtc ggcatcctgt tttcgcacaa
     6061 ccggaccgaa aacaccttcg acgacggcat cggcaactcg gcacggcttg cccacggcgc
     6121 cgttttcggg caatacggca tcgacaggtt ctacatcggc atcagcgcgg gcgcgggttt
     6181 tagcagcggc agcctttcag acggcatcgg aggcaaaatc cgccgccgcg tgctgcatta
     6241 cggcattcag gcacgatacc gcgccggttt cggcggattc ggcatcgaac cgcacatcgg
     6301 cgcaacgcgc tatttcgtcc aaaaagcgga ttaccgctac gaaaacgtca atatcgccac
     6361 ccccggtctt tcgttcaacc gctaccgcgc gggcattaag gcagattatt cattcaaacc
     6421 ggcacaacac atttccatca cgccttattt gagcctgtcc tataccgatg ccgcttcggg
     6481 caaagtccga acacgcgtca ataccgccgt attggctcag gatttcggca aaacccgcag
     6541 tgcggaatgg ggcgtaaacg ccgaaatcaa aggtttcatg ctgtccctcc acgctgccgc
     6601 cgccaaaggc ccgcaactgg aagcgcaaca cagcgcgggc atcaaattag gctaccgctg
     6661 gtaaccgccg gatatgccga aaggggtctg acgatgccac cgtgcgctgt caaacccttt
     6721 ttctgccgcc gccttgtgcc tgctccattg tctgataacc gtcaaattga tttttagccc
     6781 atgtttggtg ggtcggggaa atctatatct tcgtccgtgc cgaaatagtc tgagaccttt
     6841 gcaaaattcc tttccctccc gacagccgaa acccaaacac aggttttcgt ctattttcgc
     6901 cccaaatacc tcctaattct acccaaatac ccccttaatc ctccacggac acccgataat
     6961 caggcatccg ggctgctttt taggcggcag cgggcgcact tagcctgttg gccgctttca
     7021 aaaggttcaa acacatcgcc ttcagatggc tttgtgcact cactttg
//
LOCUS       DAWHSK010000095         6967 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.095, whole genome shotgun sequence.
ACCESSION   DAWHSK010000095 DAWHSK010000000
VERSION     DAWHSK010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6967
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.095"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(257..895)
                     /locus_tag="WIC03_001194"
     CDS             complement(257..895)
                     /locus_tag="WIC03_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HEZ3883697.1"
                     /translation="MKKINVAPENPQYRIVEIFESLQGEGWNTGMPAVFVRLGKCNLA
                     CGWCDTDYLTFGMMSLSDILGRLKAYAARNIIITGGEPTIQPHLDMLLDMLKAEGYFL
                     CIETNGLNPAPPQIDYVATSPKACYAAKYENSCIETADEVRIVADGDVLAFCENMERK
                     IRAHHYYLSPCEQDGAMNIYDTIRQIGILNSRPDAPVHWQLSVQTHKWAGIE"
     gene            complement(892..1263)
                     /locus_tag="WIC03_001195"
     CDS             complement(892..1263)
                     /locus_tag="WIC03_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEZ3883698.1"
                     /translation="MKLLSTLLVLLVTAEHFYIAWLEMTQIPSEKAAEIFKLPYEFME
                     QKRVQTLFGNQGLYNGFLGIGLVWSRFAAPDNAVYGATVLFLGFVLIAAAWGAFSSGN
                     KGILVKQGLPAFLAAAAVLAV"
     gene            complement(1532..1954)
                     /gene="queD"
                     /locus_tag="WIC03_001196"
     CDS             complement(1532..1954)
                     /gene="queD"
                     /locus_tag="WIC03_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HEZ3883699.1"
                     /translation="MKITKIFTFDSSHMLDGHDGKCQNLHGHTYKLEITVSDDLVRGG
                     PKDGMVMDFTDLKAIVKQHITDPFDHAFIYHGGNGRESQIAALLEGWNMKTLRLPCRT
                     TAENMAVEMYDRLKNAGLNVCRVKLWETPTSCAEYEGE"
     gene            complement(1961..2476)
                     /locus_tag="WIC03_001197"
     CDS             complement(1961..2476)
                     /locus_tag="WIC03_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1543 domain-containing protein"
                     /protein_id="HEZ3883700.1"
                     /translation="MPKLHMFYLGGNAGRSNIEVHDIQFAVCDDYREAIPALKAAWFG
                     DADKIHIDGWQVVEWADGYDIAVSEMPKTEMPSEHAPRLYFANVGGYRAGQLAEAHAF
                     GLFTAATPAEAKQKALQTLLTDHVQQHKDNLKDVDNLLALDRIGNFHIRLTPNPHGKP
                     AEIGFQGYLPI"
     gene            complement(2504..3163)
                     /gene="queC"
                     /locus_tag="WIC03_001198"
     CDS             complement(2504..3163)
                     /gene="queC"
                     /locus_tag="WIC03_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEZ3883701.1"
                     /translation="MSNQQALVIFSGGQDSTTCLIQAIQTYGRENVQAITFQYGQRHA
                     VELERARWIAQDLGIKQTVLDLSLMRQITHNALMDDTAAIETAGNGVPNTFVDGRNAL
                     FLLYAAIYAKGQGIRHIIAGVCETDFSGYPDCRDVFVKSMNVTLNLAMDYDFQIHTPL
                     MYLTKAQTWALADEMGALDYIREQTHTCYNGIVGGCRECPSCILRERGLAEYLESKKA
                     V"
     gene            3268..3693
                     /locus_tag="WIC03_001199"
     CDS             3268..3693
                     /locus_tag="WIC03_001199"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883702.1"
                     /translation="MPSEHAAYADLTASSAKRLLCAKAILFSSAVRTGAAVGNIFFDG
                     FADGGDFGDGAQYGFATFNAVDAASDSAGGIAGAAVLGIVEDEDFEGDLGSWADSVKM
                     VRSSNGITVVPMPSETAAVQAFVPDGKLGYNIGHQCPTY"
     gene            3708..4934
                     /locus_tag="WIC03_001200"
     CDS             3708..4934
                     /locus_tag="WIC03_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HEZ3883703.1"
                     /translation="MTFLQRLQGLADNKICAFAWFVVRRFDEERVPQAAASMTFTTLL
                     ALVPVLTVMVAVASIFPVFDRWSDSFVSFVNQTIVPQGADMVFDYINAFREQANRLTA
                     IGSVMLVVTSLMLIRTIDNTFNRIWRVNSQRPWMMQFLVYWALLTFGPLSLGVGISFM
                     VGSVQDAALASGAPQWSGALRTAATLTFMTLLLWGLYRFVPNRFVPARQAFVGALATA
                     FCLETARSLFTWYMGNFDGYRSIYGAFAAVPFFLLWLNLLWTLVLGGAVLTSSLSYWQ
                     GEAFRRGFDSRGRFDDVLKILLLLDAAQKEGKALPVQEFRRHINMGYDELGELLEKLA
                     RYGYIYSGRQGWVLKTGADSIELNELFKLFVYRPLPVERDHVNQAVDAVMMPCLQTLN
                     MTLAEFDAQAKKQQQS"
     gene            complement(4955..5237)
                     /locus_tag="WIC03_001201"
                     /pseudo
     CDS             complement(4955..5237)
                     /locus_tag="WIC03_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214428.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(5316..5450)
                     /locus_tag="WIC03_001202"
                     /pseudo
     CDS             complement(5316..5450)
                     /locus_tag="WIC03_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(5461..5907)
                     /locus_tag="WIC03_001203"
     CDS             complement(5461..5907)
                     /locus_tag="WIC03_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883704.1"
                     /translation="MLTKLKILLFLFLFVFVLAINLLFFFFSSDIESFGNYQFEYVYD
                     KGWPANYILVMKDGNEGNFDKIISGLVLEYYKEDDNIYFSYIDGQGFASDSCYYKPEI
                     LYGKIILNKNHIININSMEKNNFLSEDKIMKGTRNWLADPKNKCNI"
     gene            complement(6475..6819)
                     /locus_tag="WIC03_001204"
     CDS             complement(6475..6819)
                     /locus_tag="WIC03_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883705.1"
                     /translation="MRTHFVISNVPSTPNLLRGRQLEGKLILEVPAQVNPIPQSVLRA
                     AQEENVIIRDTTGRIYNRWWFSIRWLCRCTSCKKRTIDTNRENRGRCVSNCKCKHWIS
                     MDCQFVRAKRRS"
BASE COUNT         1647 a         1632 c         1774 g         1914 t
ORIGIN      
        1 atgctgtttc agacggcatt tcgttttccc taaccctgcc atttgcgcgc actggcacag
       61 agtatcggca aggttcaaaa gaccatcgga atttaggaag ccgggcttgt acccaacggc
      121 ggcatcttat tcagtaaggt tccgcctcaa tcaggcagcg gaaatgtggg cagtttcacg
      181 cggaagcggc acacttttcc catatgttag acatcggcaa aacggtacgc cggccctttg
      241 agttacactg cttaaactac tctattcccg cccatttgtg cgtctgcacg ctcaactgcc
      301 aatgcaccgg cgcgtcgggg cgactgttta aaataccgat ttggcggatg gtgtcgtaga
      361 tgttcatcgc accgtcttgt tcgcagggcg aaaggtagta atgatgcgcg cggattttac
      421 gttccatgtt ttcgcagaac gcaaggacat caccatcggc aacaatccgc acttcgtcgg
      481 ctgtttcgat acagctattt tcatatttgg cggcgtagca ggctttgggg ctggtggcga
      541 catagtcgat ttgcggcggc gcgggattga gtccgttggt ttcgatacag aggaaataac
      601 cttccgcctt gagcatgtcc agcagcatat cgagatgcgg ctggatggtc ggttcgccgc
      661 cggtgatgat gatgttgcgg gcggcatagg ctttcagacg gcctaagatg tcggacaggc
      721 tcatcatgcc gaatgtcaaa taatcggtat cacaccagcc gcaggcgagg ttgcatttgc
      781 ccaagcggac gaaaacggcg ggcatgcccg tgttccagcc ttcgccttgc aggctttcaa
      841 agatttcgac gatgcggtat tggggatttt cgggggcaac attgattttt ttcataccgc
      901 caacaccgcc gccgctgcca aaaatgcggg caaaccttgt ttgacgagta tgcctttgtt
      961 gccggaagag aacgcgcccc acgcggcggc aatcaggacg aagccgagaa acagtaccgt
     1021 tgcgccgtac accgcgttat ccggagcggc aaaccgcgac cacaccagcc cgatgccgag
     1081 aaagccgtta tacagccctt ggttgccgaa cagggtctgc acgcgctttt gttccataaa
     1141 ttcataaggc agcttgaata tttccgccgc tttttcgctg ggaatctgcg tcatttcaag
     1201 ccaggcgatg tagaaatgtt cggcggtgac aaggaggact aagagggtgg agagaagttt
     1261 catgggtatt ccttttaatg ggtgtatttg atatggaggg tcgtctgaaa aaacggggaa
     1321 tccgtacagg agaaaaaacc gttttacggt aaattcaaat aaggacactc taaaccgtcc
     1381 tttccgcgca ggcgggaatc caaagatttg gaattgctgc aatttttaaa tattcctgaa
     1441 aaaccaaggt ctggattccc gcctgcgcgg gaatgacgcg gttgggcatg tcttattgga
     1501 atttactata tcgatacgtt caagatattc cctactcccc ttcatactcc gcacacgatg
     1561 tcggcgtttc ccacaatttg acgcggcaca cgttcagccc cgcgtttttc agacggtcgt
     1621 acatttcgac cgccatattt tcggcagtgg tgcggcaggg caggcgcagg gttttcatgt
     1681 tccagccttc caagagtgcg gcgatttgag attcgcgccc gttgccgccg tggtagatga
     1741 aggcgtggtc gaaggggtcg gtaatgtgtt gtttgacaat ggctttcaag tcggtaaagt
     1801 ccatcaccat accgtctttc ggcccgcctc tgacgaggtc gtctgaaacg gtgatttcga
     1861 gtttgtaggt atgcccgtgc aggttttggc atttgccgtc atgcccgtcg agcatatgag
     1921 aggagtcgaa ggtgaagatt ttggtgattt tcatgggttc tcaaatgggc aaatagcctt
     1981 gaaagccgat ttcggcgggt ttgccgtgcg gattcggggt caggcggata tggaaattgc
     2041 cgatgcggtc gagcgcaagc aggttgtcca cgtcttttaa gttgtcttta tgttgctgaa
     2101 catggtcggt caacagggtt tgcagggctt tttgtttggc ttcggcaggc gtggcggcgg
     2161 tgaacagccc gaacgcgtgc gcctcggcaa gctgccccgc gcgataaccg ccgacattgg
     2221 caaaatacag gcgcggggcg tgttcagacg gcatttccgt tttgggcatt tcggatacgg
     2281 cgatgtcgta accgtccgcc cattcgacaa cctgccaacc gtcgatgtgg attttgtccg
     2341 catcgccgaa ccatgcggct ttgagtgcgg ggatggcttc gcggtagtcg tcgcacacgg
     2401 caaattggat gtcgtgcact tcgatattcg acctgccggc attgccgccg aggtaaaaca
     2461 tatggagctt gggcatatcg tattccttat ggtttgcgcg tgttcagacg gcctttttac
     2521 tttccagata ttccgccagc ccgcgttcgc gcaagataca gctcgggcat tcgcggcagc
     2581 cgccgacgat gccgttatag caggtgtggg tttgctcgcg gatatagtcc agcgcgccca
     2641 tttcgtccgc caacgcccaa gtttgcgcct tggtcagata catcagcggc gtgtggattt
     2701 gaaaatcata gtccatcgcc aaattaaggg taacgttcat cgatttgaca aacacgtcgc
     2761 ggcagtcggg atagccggag aagtcggttt cgcacacgcc cgcgatgatg tgccgtatcc
     2821 cctgcccttt ggcgtaaatc gcggcgtaaa gcaggaaaag cgcgttgcgc ccgtccacaa
     2881 aggtattggg aacaccgttg ccggcggttt cgatggcggc ggtatcgtcc atcagggcat
     2941 tgtgcgtaat ctgccgcatc aggctcaagt cgagtacggt ttgtttgatg cccaaatcct
     3001 gcgcaatcca gcgggcgcgt tccagctcga cggcatggcg ttgcccgtat tggaaagtga
     3061 tggcttggac gttttcgcgc ccgtaggttt ggattgcctg aatcaggcag gtggtcgaat
     3121 cctgaccgcc cgaaaagatg accaaggctt gttggtttga catatcgaat cctagttttg
     3181 gtaacgcggg aggtttgcga accgcgaagt tgaacagtaa agatgcggat tatacaggca
     3241 aacgcgttca gacggcatct gaacgccatg ccgtctgaac acgcggctta tgccgacttg
     3301 acggcgagtt ccgccaagcg tttgctttgt gcgaaggcga tattgttttc ttcggcggtc
     3361 aggacgggag ccgctgtcgg gaatatcttt tttgacggct tcgcagacgg cggagacttt
     3421 ggggacggtg cgcaatacgg cttcgcaacc ttcaacgctg tcgatgccgc gagcgattcg
     3481 gcgggcggga ttgcgggggc tgccgttttg ggaatagtag aggacgagga ttttgagggg
     3541 gatttgggtt catgggcgga ttccgttaaa atggttcggt cttcaaacgg cataacagtt
     3601 gttccgatgc cgtctgaaac agccgcagtt caggcatttg ttcctgacgg caaacttggc
     3661 tataacatcg ggcatcaatg cccaacttac tgaccgggca agaaattatg acctttttac
     3721 aacgtttgca aggtttggca gacaataaaa tctgtgcgtt tgcatggttc gtcgtccgcc
     3781 gctttgatga agaacgcgta ccgcaggcgg cggcaagcat gacgtttacg acgctgctgg
     3841 cactcgtccc cgtgctgacc gtgatggtgg cggtcgcttc gattttcccc gtgttcgacc
     3901 gctggtcgga ttcgttcgtc tccttcgtca accaaaccat tgtgccgcag ggcgcggaca
     3961 tggtgttcga ctatatcaat gcgttccgcg agcaggcgaa ccggctgacg gcaatcggca
     4021 gcgtgatgct ggtcgttacc tcgctgatgc tgattcggac gatagacaat acgttcaacc
     4081 gcatctggcg ggtcaattcc cagcgtccgt ggatgatgca gtttctcgtc tattgggctt
     4141 tactgacgtt cgggccgctg tctttgggcg tgggcatttc ctttatggtc ggctcggtac
     4201 aggatgccgc gcttgcctca ggtgcgccgc agtggtcggg cgcgttgcga acggcggcga
     4261 cgctgacctt catgacgctt ttgctgtggg ggctgtaccg cttcgtgcca aaccgcttcg
     4321 ttcccgcgcg gcaggcgttt gtcggggctt tggcaacagc gttttgtctg gaaaccgcgc
     4381 gctccctctt cacttggtat atgggcaatt tcgacggcta ccgctcgatt tacggcgcgt
     4441 ttgccgccgt gccgtttttt ctgttgtggc tgaacctgtt gtggacgctg gtcttgggcg
     4501 gcgcggtgct gacttcttca ctctcctact ggcagggaga agcgttccgc aggggcttcg
     4561 actcgcgcgg acggtttgac gacgtgttga aaatcctgct gcttctggat gcggcgcaaa
     4621 aagaaggcaa agccttgcct gttcaggagt tcagacggca tatcaatatg ggctacgacg
     4681 agttgggcga gcttttggaa aagctggcgc ggtacggcta tatctattcc ggcagacagg
     4741 gttgggtgtt gaaaacgggg gcggattcga ttgagttgaa cgaactcttc aagctcttcg
     4801 tttaccgtcc gttgcctgtg gaaagggatc atgtgaacca agctgtcgat gcagtaatga
     4861 tgccgtgttt gcagactttg aacatgacgc tggcagagtt tgacgctcag gcgaaaaaac
     4921 agcagcaatc ttgagtattt ttgaaactgt atttttatcc taaagacatt ttcctatctg
     4981 ctgattccac cgtttccttt ctgtgtgccg ccataatttt tatgatagac aagccattca
     5041 agtttgcatt gactgctttt tcattggcaa tatccaaatg gctggtcgct gcatctaaaa
     5101 ataggatttt cggttcgcaa tattaaggcc cgcgccaata cgatgcgttg tttttgtccg
     5161 cctgacagtg cgctgcccat atcgccgatc aaggtttcat agcccatagg catggcggag
     5221 atttcttcgt gaatcatagc aaggcaggcg caatgttcga tttttcctct atccggttcc
     5281 gtatcgaaaa aacaaggggc tgtactagat tagccctaaa tcccacacca atcccgcagg
     5341 attttaagct gttgagacgg tgtgccgaag ttaaatcgaa attcgcattc tttcaagaac
     5401 agcgggaaag atttacgatc gattccgttg tattttcgca agacgcgttt agtctagagt
     5461 ctatatatta catttatttt tagggtctgc tagccaattt cttgttccct tcattatttt
     5521 atcttctgaa agaaaattat ttttttccat gctattaata ttaatgatat gatttttatt
     5581 taaaataatt tttccatata aaatttccgg tttgtaatag caagagtctg aagcaaatcc
     5641 ttgcccgtca atataagaaa aataaatgtt atcatcctcc ttataatatt ctaaaaccaa
     5701 tccggatatt attttatcaa aattcccttc atttccatct ttcatgacta aaatataatt
     5761 agcaggccaa cctttatcgt aaacatattc aaactgatag ttcccgaaac tctcgatatc
     5821 cgaactaaaa aagaagaaaa gcaaattaat agccaataca aaaacaaata agaacaaaaa
     5881 tagcaaaatt ttcaacttag ttaacaatat ttacctctcc tttaaattca atcctgaaag
     5941 gtacccctta ccctgggcta ccaattatag tttccatatt tctaaatatt gtttttacat
     6001 tacttttttc tccccccaaa ggaatgcatt ttaaaatcat gcttttcagg tgctaatcga
     6061 tacttaccat taacatcttt aatcacagat atatttccat gtatagccca acgtgaaaaa
     6121 tctgagtatt atatacagtt ataccttact gaaacaaagc tagctgtaca tatctgtacc
     6181 atatttttac tacaaacaaa aaatatttat aattattaag gataagataa aaacccccaa
     6241 cgccagatta attcttaata atttcaaata aaaaatattt gagatttttt cttttccaaa
     6301 taatatattg aaatatttaa agttaaggta gcttgattta attagaaaat caaaaaaacc
     6361 attaattaat ttttcttctt taaatatttt atatgatatt ataaaattaa ataacattgc
     6421 gaaaacatat gaaatcatca aaataccaaa tatatagtgg gaatgcatta ttatttagct
     6481 ccttcttttg gctctgacaa attgacagtc cattgatatc caatgcttgc atttgcaatt
     6541 gctgacgcac ctgcccctgt tttccctgtt agtatcaatg gttcttttct tgcaagatgt
     6601 gcacctgcac aaccacctaa tactaaacca ccacctattg taaatccttc ctgttgtatc
     6661 tctaatgata acattttctt cttgtgccgc ccttaataca gattgtggaa ttggattgac
     6721 ctgagcagga acttctaaaa taagttttcc ttcaagctgt cttcctctta gcagattagg
     6781 tgtacttgga acattggaaa tgacaaagtg agtacgcaaa ccacctgaag gcgatgtgtt
     6841 tgaacctgtt gaaagcggcc aacaggctaa atgcgcccgc tgccgcctaa aaggcagccc
     6901 ggatgcctga ttatcgggtg tccgaggagg attaaggggg tatttgggta aaattaggag
     6961 gtatttg
//
LOCUS       DAWHSK010000096         6758 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.096, whole genome shotgun sequence.
ACCESSION   DAWHSK010000096 DAWHSK010000000
VERSION     DAWHSK010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6758
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.096"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..203
                     /locus_tag="WIC03_001205"
     CDS             <1..203
                     /locus_tag="WIC03_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEZ3883706.1"
                     /translation="MPSEGAAGGTAVGGILDKMGFDPIHPDVLAGQLAMPAADLYAAL
                     LELELDGSVAAMPGGRYQRIRT"
     gene            229..690
                     /locus_tag="WIC03_001206"
     CDS             229..690
                     /locus_tag="WIC03_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein"
                     /protein_id="HEZ3883707.1"
                     /translation="MTEVIAYLIEHFQDFDTCPPPEDLGMLLEEAGFDTMEIGNTLMM
                     MEVLLNSSEFSAEPADSGALRVYSKEETDNLPQEVMGLMQYLIEEKAVSCEQREIIIH
                     ALMHIPGDEITVDTAKVLTLLLLWANKSELPVLVGDELMSALLLDNKPTMN"
     gene            762..3068
                     /gene="topA"
                     /locus_tag="WIC03_001207"
     CDS             762..3068
                     /gene="topA"
                     /locus_tag="WIC03_001207"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEZ3883708.1"
                     /translation="MAKNLLIVESPSKAKTLKKYLGGDFEILASYGHVRDLVPKSGAV
                     DPDNGFAMKYQLISRNGKHVDAIVAGAKEAENIYLATDPDREGEAISWHLLEILKSKR
                     GLKNIKPQRVVFHEITKNAVLDAVAHPREIEMDLVDAQQARRALDYLVGFNLSPLLWK
                     KIRRGLSAGRVQSPALRLICERENEIRAFEAQEYWTVHLDSHKGRSKFTAKLAQYNGA
                     KLEQFDLPNEAAQADVLKELEGKEAVVTAIEKKKRSRNPAAPFTTSTMQQDAVRKLGF
                     TTDRTMRTAQQLYEGIDVGQGAIGLITYMRTDSVNLADEALTEIRHYIENKIGKEYLP
                     SAAKQYKTKSKNAQEAHEAIRPTSVYRTPESVKPFLSADQFKLYQMIWQRTVACQMTP
                     AKFDQTTVDITVGKGVFRVTGQVQTFAGFLSVYEESSDDEEGEDSKKLPEMSEGDKLP
                     VDKLYGEQHFTTPPPRYNEATLVKALEEYGIGRPSTYASIISTLKDREYVTLEQKRFM
                     PTDTGDIVNKFLTEHFAQYVDYHFTAKLEDQLDEIADGKRQWIPLMDKFWKPFIKQVE
                     EKEGIERAKFTTQELDETCPKCGEHKLQIKFGKMGRFVACAGYPECSYTRNVNETAEE
                     AAERIAKAEAEQAELDGRECPKCGGRLVYKYSRTGSKFIGCANYPKCKHVEPLEKPKD
                     TGVQCPQCKKGNLVERKSRYGKLFYSCSTYPDCNYATWNPPVAETCPKCAWPVLTIKT
                     TKRWGVEKVCPQKECGWKEQIEPPAPKE"
     gene            complement(3146..3412)
                     /locus_tag="WIC03_001208"
     CDS             complement(3146..3412)
                     /locus_tag="WIC03_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003786399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEZ3883709.1"
                     /translation="MASSEPFPCSACGLCCQKVNLSEQTIYLDRGDGTCRHFDDSTRL
                     CTIYETRPLVCRVEDYYHHYLSEQMSWQDFIQINIEVCKQFQSE"
     gene            complement(3412..3831)
                     /locus_tag="WIC03_001209"
     CDS             complement(3412..3831)
                     /locus_tag="WIC03_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883710.1"
                     /translation="MGKIQILNKLPTKNPATIPFDAASNSFFRILEARNEYKKIAAQE
                     KTKREVIGAWRDVRLAELKNQQEILRLYLENTFKERRKMIDGLFEALDKGMEAGNIHV
                     IDTAINGIVSIAKDSPLKQVDNLIAALKNDNVKVIDF"
     gene            complement(3831..4778)
                     /locus_tag="WIC03_001210"
     CDS             complement(3831..4778)
                     /locus_tag="WIC03_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883711.1"
                     /translation="MAIPVLLWGAAAALAATGIVKGAAALSDIDDAKEIGERAERRYK
                     RRKRELNSTKDETNLAFEELGQFKVDIFNNQIRHIVQVLNNTSNKHAKSELSGFNSTF
                     DIAELKEMETMVQTSLELSTNLGSSAITGALTGFGVYGAVPMIATASTGTAISTLSGA
                     AATNATLAWLGGGSLAAGGFGMAGGMVALGGIVAGPALAIGGFMLASKAEEALTEARA
                     YSAEIDEAIDEMDLITEGLEGLKENAREIRHTLEEMVKRFEKVRVDHADHPDFHTMLV
                     IGKAIKNLLNTPIMEEDGSPVANIRHQCEGCLNIELGTV"
     gene            4935..5312
                     /locus_tag="WIC03_001211"
     CDS             4935..5312
                     /locus_tag="WIC03_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ3883712.1"
                     /translation="METHEKIRLMRELNKWSQEDMAEKLAMSAGGYAKIERGETQLNI
                     PRLEQLAQIFKIDMWDLLKSGGGGMVFQINEGDSGGDIALYASGDVSMKIEFLKMELK
                     HCKEMLEQKDKEIELLRKLTETV"
     gene            5401..5970
                     /gene="rsmD"
                     /locus_tag="WIC03_001212"
     CDS             5401..5970
                     /gene="rsmD"
                     /locus_tag="WIC03_001212"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEZ3883713.1"
                     /translation="MAAGKHTKHSNQVRIIGGQCRGRKLSFASANGLRPTPDSVREKL
                     FNWLGQDLTGKTVLDLFGGSGALGIEAASRNAKRVLISDNNRQTVQTLQKNSRELGLG
                     QVQIVCSDGIAYLKTVSEQFDVVFLDPPFAWQDWQILFDALKPCLNPRAFVYLEAGRL
                     PDIPDWLTEYREGKSGQSTFELRVFQVAE"
     gene            6093..6344
                     /locus_tag="WIC03_001213"
     CDS             6093..6344
                     /locus_tag="WIC03_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HEZ3883714.1"
                     /translation="MSLFITDECINCDVCEPECPNDAISQGEEIYEINPNLCTQCVGH
                     YDEPQCQQVCPVDCILIDEEHPETHDELMAKYEKIIQFK"
     gene            6441..6524
                     /locus_tag="WIC03_001214"
     tRNA            6441..6524
                     /locus_tag="WIC03_001214"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6475..6477,aa:Tyr,seq:gta)
     gene            6555..6628
                     /locus_tag="WIC03_001215"
     tRNA            6555..6628
                     /locus_tag="WIC03_001215"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6587..6589,aa:Gly,seq:tcc)
     gene            6638..6712
                     /locus_tag="WIC03_001216"
     tRNA            6638..6712
                     /locus_tag="WIC03_001216"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6670..6672,aa:Thr,seq:ggt)
BASE COUNT         1891 a         1733 c         1584 g         1550 t
ORIGIN      
        1 aaatgccgtc tgaaggggcg gcaggcggca cagccgtcgg cggcatactg gataaaatgg
       61 gtttcgaccc catccatccc gacgtgcttg ccggacagtt ggctatgcct gccgcagatt
      121 tgtatgccgc actgttggaa ttggaattgg acggcagcgt tgccgcaatg cccggcggca
      181 gataccagcg tatccgaact tgaacgcact ttatattaag gaacacgaat gaccgaagtc
      241 atcgcctacc tcatcgaaca tttccaagat ttcgatacct gtccgccgcc cgaagacttg
      301 ggtatgctgc ttgaagaagc gggtttcgat acgatggaaa tcggcaacac cctgatgatg
      361 atggaagtat tgctcaacag ctccgaattt tccgccgaac ctgccgacag cggcgcattg
      421 cgcgtgtaca gcaaagaaga aaccgacaac ctgccgcagg aagtgatggg gctgatgcag
      481 tatctgattg aagaaaaagc cgtcagctgc gaacagcggg aaatcatcat ccacgcgctc
      541 atgcacattc cgggcgacga aattaccgta gataccgcca aagtgctgac cctgctgctt
      601 ttatgggcaa acaagagcga gctgcccgtg ttggtcggcg acgagctgat gagcgcgctt
      661 ttactcgaca acaaacccac gatgaactga agcggcttca gacggcccgc ccgagtccgt
      721 ctgaaacgtc ggcatcaaaa ccaccatcca gagaacgaca aatggcgaaa aacctattaa
      781 tcgtcgaatc cccgtccaaa gccaaaaccc tgaaaaaata tttgggcggc gattttgaaa
      841 tccttgcatc ctacggacac gtccgcgacc tcgtccccaa aagcggcgcg gtcgatcccg
      901 acaacggctt tgcgatgaaa taccaactca tcagccgcaa cggcaaacac gtcgatgcca
      961 tcgtcgccgg agccaaagaa gccgaaaaca tctacctcgc caccgacccg gatagggaag
     1021 gcgaagcgat ttcttggcat cttttggaaa tcctcaaatc caaacgcggc ctgaaaaaca
     1081 tcaagccgca gcgtgtcgtg ttccacgaaa tcaccaaaaa cgccgtgctc gatgccgttg
     1141 cccatccgcg cgaaatcgaa atggacttgg tcgatgcgca acaagcccgt cgcgctttgg
     1201 actatttggt cggctttaac ctttcgccat tgttatggaa aaaaatccgc cgcggtttga
     1261 gcgcgggtcg tgtgcaaagt cccgctttgc gcctgatttg tgagcgtgaa aacgaaatcc
     1321 gcgcgtttga agcgcaggaa tattggacgg tgcatctcga cagccacaaa ggccgcagca
     1381 agtttaccgc caaactcgcc caatacaacg gcgcgaaact cgaacaattc gacctgccga
     1441 acgaagccgc gcaagccgac gtgttgaaag aactcgaagg caaagaggcc gtcgttaccg
     1501 ccattgaaaa gaaaaagcgc agccgcaatc ccgccgcgcc gttcaccact tctaccatgc
     1561 agcaggatgc cgtgcgcaaa ctcggcttca ccaccgaccg caccatgcgt accgcccaac
     1621 agctttatga aggtatagac gtagggcagg gcgcgatcgg tctgattacc tatatgcgta
     1681 ccgacagcgt gaatttggcg gatgaagcct taaccgaaat ccgccattac atcgaaaaca
     1741 aaatcggcaa agaatatctg ccgagtgccg ccaaacaata caaaaccaaa tccaaaaacg
     1801 cccaagaagc gcacgaagcc atccgcccga cttccgtgta ccgcacgccc gaaagcgtca
     1861 aacccttcct gagcgcagac cagttcaaac tctatcaaat gatttggcag cgtaccgtcg
     1921 cctgtcagat gacgcccgcc aaattcgacc aaaccaccgt cgatattacc gtcggcaaag
     1981 gcgtattccg cgtaaccgga caagtgcaga ctttcgcagg cttcctcagc gtttacgaag
     2041 aaagcagcga cgatgaagaa ggcgaagaca gcaaaaaact gcccgaaatg agcgaaggcg
     2101 acaaattgcc cgtggacaaa ctctacggcg aacaacactt taccactccg ccgccacgct
     2161 acaacgaagc cacgctggtt aaagccctcg aagaatacgg catcggccgc ccctcgacct
     2221 acgccagcat catctccacg ctcaaagacc gcgaatacgt tacccttgag caaaaacgct
     2281 tcatgcccac cgacacaggc gacatcgtca ataaattcct gaccgaacac ttcgcccaat
     2341 acgtcgatta ccacttcact gccaaactcg aagaccagct tgacgaaatt gccgacggca
     2401 aacgccaatg gattcccttg atggacaaat tctggaaacc gttcatcaaa caagtggaag
     2461 aaaaagaagg catcgaacgc gccaaattta ccacgcagga acttgacgaa acctgcccca
     2521 aatgcggcga acacaaactg caaatcaagt tcggcaaaat gggtcgcttc gttgcctgcg
     2581 ccggttatcc agagtgcagc tacacccgca acgtcaacga aactgccgaa gaagccgccg
     2641 agcgcatcgc caaagccgaa gcagagcaag ccgaacttga cgggcgcgaa tgccccaaat
     2701 gcggaggacg gctggtgtac aaatacagcc gaaccggcag caaattcatc ggctgcgcca
     2761 actatccgaa atgcaaacac gtcgagccgt tggaaaaacc gaaagacacc ggcgtccaat
     2821 gcccgcaatg caaaaaaggc aacctcgtcg agcgcaaatc ccgctacggc aaactgtttt
     2881 acagttgcag cacctatccc gactgcaact acgccacctg gaacccgccc gttgcagaaa
     2941 cctgtccaaa atgcgcgtgg ccggttttga ccatcaaaac caccaaacgc tggggtgtgg
     3001 aaaaagtctg cccgcaaaaa gaatgcggct ggaaagaaca gattgaaccg cctgcaccga
     3061 aagagtgaag cggttggggt tgagatgaaa gaaaatgccg tctgaaaggg gtttcagacg
     3121 gcattttctt catgtgaaaa caaacctact cagattgaaa ctgtttacac acttctatat
     3181 taatctgtat aaagtcctgc cacgacattt gttctgataa gtaatggtga taatagtcct
     3241 ccacacgaca gaccaaaggg cgcgtttcat agatagtgca taagcgcgtt gaatcatcga
     3301 aatggcggca ggtaccatct ccccgatcca ggtatatggt ttgttcgctt aaattgacct
     3361 tttgacagca caaaccgcaa gcagagcatg ggaagggttc tgatgaggca atcaaaaatc
     3421 gattactttc acattgtcgt ttttcaaagc tgcaatcaga ttgtccacct gtttcagtgg
     3481 agaatctttg gcaatgctca caatgccgtt aattgctgta tcaatgacgt ggatatttcc
     3541 cgcttccatt cctttgtcca atgcctcaaa taaaccgtca atcattttgc gacgttcttt
     3601 gaacgtattt tccagataca agcgcaaaat ttcttgttgg tttttcaatt ctgctaatcg
     3661 gacatcacgc catgcaccaa tgacttcacg tttggttttc tcttgtgcag caattttctt
     3721 atattcgttg cgtgcttcta agatgcgaaa aaacgaatta gaagctgcat caaacggaat
     3781 ggttgctgga ttctttgttg gtagcttatt aaggatttgg atttttccca ttatactgta
     3841 cctaattcga tattcagaca accttcgcat tgatgacgga tattggctac aggagatccg
     3901 tcctcttcca taataggcgt attcagcaga tttttaatcg ccttgccgat gaccagcatg
     3961 gtatggaaat caggatggtc ggcatggtcg acacgaacct tttcgaaacg tttaaccatt
     4021 tcttctaagg tatggcgtat ttcccttgcg ttttccttca acccttctaa tccttctgta
     4081 attaaatcca tctcgtcaat agcctcatcg atttcagcac tatatgctcg tgcttccgtc
     4141 agtgcctctt ccgccttgct tgccagcata aatccaccga ttgctaaagc aggtcctgcc
     4201 acgatgccac ccaaagccac catgccgcct gccataccga atccacctgc tgccaatgag
     4261 ccacctccga gccaagcaag ggttgcattg gttgccgcag cacctgaaag ggtggagatt
     4321 gctgttcctg tggaagcagt tgcaatcata ggtactgcac cgtatacgcc aaagcctgtc
     4381 agtgcgccag taatggcact actgccgaga ttggtgctta actctaaaga agtttgcacc
     4441 atagtttcca tttcttttaa ttcagcaata tcaaatgtac tgttaaaacc cgacagttcg
     4501 cttttcgcat gcttgttgct ggtgttgttt aggacttgaa caatatgtct gatttgattg
     4561 ttaaaaatat caaccttaaa ctgtcccagc tcctcaaaag caaggttggt ttcgtcttta
     4621 gtcgaattca attctctttt cctgcgttta taacggcgtt ctgcacgctc acctatttct
     4681 tttgcatcat ctatatcgct gagtgcagct gctcctttga caatacctgt tgcagcaagg
     4741 gcggcggctg caccccaaag taaaactgga atagccatgt gtaatttcct aaagtgtata
     4801 aaaagttaaa aatcttgaag tttcaatgtg tattgtaatt ttaaaattct attaaaaata
     4861 catttgccat tccagcaaac taaagtttaa aatctatcca aataatttaa ccatctatac
     4921 agaaagttca gctcatggaa acccacgaaa aaatccgcct gatgcgcgaa ttgaacaaat
     4981 ggtcgcagga ggatatggcg gaaaagctgg cgatgtcggc aggcgggtat gccaaaatcg
     5041 aacggggcga aacgcagttg aatatcccgc gtttggagca gttggctcag attttcaaaa
     5101 tcgatatgtg ggacttgctc aaatcgggcg gcggcgggat ggtgtttcag attaatgaag
     5161 gtgatagtgg tggcgatatt gcgttgtatg cgtcaggtga tgtttcgatg aaaatagaat
     5221 ttttaaaaat ggagttgaaa cattgtaaag aaatgttgga acaaaaagac aaagaaatcg
     5281 agttgctccg caagctgacc gaaaccgttt aaacagatat gccgtctgaa aaaagttttc
     5341 agacggcata ttctttgaca ggtcttgtat aataccgttt gaacttacag gtttttgatt
     5401 atggcggcag gcaaacatac caaacacagc aaccaggtac gcattatcgg cgggcaatgt
     5461 cggggcagga aattgagttt tgcatccgcc aacggactgc gtccaacccc cgatagcgtg
     5521 cgtgaaaagc tgtttaactg gctgggacag gatttgacgg gtaaaacggt tttggatctc
     5581 ttcggaggca gcggcgcact cggtatagaa gccgcttcgc gcaacgccaa acgcgtgctg
     5641 atttcggata acaaccgcca aaccgtgcag accttgcaga aaaacagtcg cgaactgggt
     5701 ttggggcagg tgcaaatcgt ctgttcagac ggcatcgcat atttgaagac cgtatccgaa
     5761 cagtttgatg tcgtctttct cgacccgccg tttgcatggc aggactggca aatcctgttc
     5821 gatgccttga agccgtgcct gaacccccgg gcattcgtct atctcgaggc gggtaggctg
     5881 ccggatatcc ccgattggct gacggaatat agagaaggga aatcagggca gagtacattc
     5941 gaattaaggg ttttccaagt ggctgaataa tatgcgcttt gataatcatt tccgagttgt
     6001 aaacattcgt ttgcaaccgt ccggttcaaa aaaaccttgt gctataatcc gcgcccgccc
     6061 ggttttgata atttagtgga aaaggaaaag aaatgtcgct ttttattacc gacgagtgca
     6121 tcaactgcga cgtatgcgaa cccgaatgcc ccaatgatgc catttcccaa ggcgaggaaa
     6181 tttacgaaat caaccccaac ctctgcacgc agtgcgtcgg acactacgat gagccgcagt
     6241 gccagcaggt ttgcccggtg gactgcatcc tgattgacga agaacatccc gaaacccatg
     6301 acgagttgat ggcgaaatac gaaaagatta tccagtttaa ataaattctt tttaaaacat
     6361 caaattatgt ctgttttgaa ataaaatcaa aaaaaaactt gacggaaaag caagccgcta
     6421 ataaactaac gttctctttt ggagggattc ccgagcggtc aaagggggca gactgtaaat
     6481 ctgttgcgaa agcttcgaag gttcgaatcc ttctccctcc accaaaattc ttacttgggg
     6541 cagtagcgag taatgcgggt gtagttcaat ggtagagcag aagccttcca agcttacggt
     6601 gagggttcga ttcccttcac ccgctccaaa caattaggcc catgtagctc aggggtagag
     6661 cactcccttg gtaagggaga ggccggcagt tcaaatctgc ccatgggcac catctctcga
     6721 ttattcattt ctttaaggct tagatatata ggatattg
//
LOCUS       DAWHSK010000097         6707 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.097, whole genome shotgun sequence.
ACCESSION   DAWHSK010000097 DAWHSK010000000
VERSION     DAWHSK010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6707
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.097"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(133..1266)
                     /gene="nrdB"
                     /locus_tag="WIC03_001217"
     CDS             complement(133..1266)
                     /gene="nrdB"
                     /locus_tag="WIC03_001217"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HEZ3883715.1"
                     /translation="MSYSTFPKTKNDALNEPMFFGQPVNVARYDQQKYEVFEKLIEKQ
                     LSFFWRPEEIDVSRDRIDYANLPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLVSI
                     PELETWIETWSFSETIHSRSYTHIIRNIVNDPSVVFDDIVENEYITARAEDIACYYDD
                     LIEYTQYYNLLGEGMHNVGGKPITVSLRGLKKKLYLCLMCVNVLEAIRFYVSFACSFA
                     FAERELMEGNAKIIKLIARDEALHLTGTQHMLNLMRSGVDDSEMAEIAAELQDECFQL
                     FKKAAEQEKEWAAYLFKDGSMIGLNKEILSQYVEYITNLRMQAVGLPAGFEGANQNPI
                     PWINAWLSSDNVQVAPQEVEISSYLIGQIDSEVNTDDLGDFEL"
     gene            complement(1327..2514)
                     /locus_tag="WIC03_001218"
     CDS             complement(1327..2514)
                     /locus_tag="WIC03_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HEZ3883716.1"
                     /translation="MNDLAAQTIQVVNKSDDLVASAIQTANKSIAVYCRYIRSNDVGT
                     TGSHQSGFYIQKHFSDDLFDVVCQKGTNKTISIKINWQDGSVTNSNFKYYGQGTRNEA
                     RITGFGKNFEFLSDKYSGSLLVLCRACYKDLLFHAFVLSSDEDIEIFIAETNILPGSL
                     YLPKKQEVEDNLTKLFSLFPNFPKTEEMAVLARENIALNKTNVSNVLKQWVSKEYELF
                     SIFEKREFEIIKSKITDLDSFINFAHSFTNRRKARAGKSLELHLSRIFDEFSLKFETQ
                     AKTEGKKKPDFLFPGSEEYHAMDESGNFIFQTEKLTMLGSKTTCKDRWRQVLNEADRI
                     PHKHLFTLQEGISDTQIQEMSDENLTLVVPKESVKTFGTFGKTHVLTLENFIKYIKSQ
                     QVS"
     gene            complement(2507..3520)
                     /gene="dcm"
                     /locus_tag="WIC03_001219"
     CDS             complement(2507..3520)
                     /gene="dcm"
                     /locus_tag="WIC03_001219"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEZ3883717.1"
                     /translation="MKNKIQNQLDNIVTLNNEKFTFIDLFAGIGGFRIAMENVGGRCV
                     FSSEWDDKARQTYQVNFNDIPYGDITLKETKAAIPSKFDVLTAGFPCQPFSIAGVSKK
                     KSLGRETGFLDKAQGTLFFDVAEIIGKHRPKIFLLENVKNLVSHDKGNTFKVIKGTLE
                     ELDYQIFYQVMNAKYYVPQNRERIFIVGFDRQYFNKEINFNFPSPPESQPKLKQILED
                     DVDNSFTLSDNLWLYLQNYAKKHKAKGNGFGFGLVDLDGISRTLSARYYKDGSEILIP
                     QKGKNPRKLTPRECSRLMGFPKDFVIDAVSKTAAYKQFGNSIAVPLVQAIAKQIINEL
                     KNE"
     gene            complement(3578..5857)
                     /gene="nrdA"
                     /locus_tag="WIC03_001220"
     CDS             complement(3578..5857)
                     /gene="nrdA"
                     /locus_tag="WIC03_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HEZ3883718.1"
                     /translation="MNTPTDLKVTKRDGRLEAIDLDKIHRVVTWAAEGLENVSVSQVE
                     LKSHIQFYNGIRTDDIHETIIKAAADLISEDTPDYQYLAARLAIFHLRKIAYGEYEPP
                     HLYDHVKKLTDAGKYDRHILEDYSREEFDELNAYIDHERDMSFSYAAVKQLEGKYLVQ
                     NRVTSQIYETPQFLYVLVAMCLFSKYPKETRLDYVKRFYDAVSTFKVSLPTPIMSGVR
                     TPTRQFSSCVLIECDDSLDSINATTSAIVKYVSQRAGIGINAGRIRGLDSEIRGGEAR
                     HTGCIPFFKMFQAAVKSCSQGGVRGGAATLFYPLWHIEAESLLVLKNNRGVEDNRIRQ
                     LDYGVQINRLLYTRLIKGGNITLFSPNEVPGLYEAFFADQDEFERLYTKYEQDPNIRK
                     RIIPAADLFSTLMQERAGTGRIYIQNVDHCNTHSPFDPCVAPVHQSNLCMEIALPTKP
                     LDNINDPNGEIALCTLSAFNLGALNSLDELEGLADLTVRALDALLDYQGYPVEAARTS
                     TMGRRSLGIGVINYAYYLAKNGVRYSDGSALGLTHRTFEAIQYYLLKASANLAKEYGA
                     CTLFNQTVYSQGKLPIDTYKKDLDAVCGEPLHYDWESLRADIVKYGLRNSTLTALMPS
                     ETSSQIANATNGIEPPRGLVTVKASKDGILKQVVPEFETLKNAYETLWQLPGNEGYLK
                     LVGVMQKFVDQSISANTAYDPGKFEGGKVSMKQMLKDLLTAYKYGVKTLYYHNTRDGA
                     DDTQTDIQDDGCAGGACKI"
     gene            6386..6697
                     /locus_tag="WIC03_001221"
     CDS             6386..6697
                     /locus_tag="WIC03_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein"
                     /protein_id="HEZ3883719.1"
                     /translation="MLAILLSAILGLVSTTAAAGTSEPAHRHTKHIRKANKQMLHPEC
                     RKYLERRAAWYRSQGNVQELRENKKARKAFRSLPYAEQKIQCRAAYEAFDDFDGGSFR
                     R"
BASE COUNT         1797 a         1496 c         1415 g         1999 t
ORIGIN      
        1 gatttttgtt aatccactat aatccactat aaaaggaaca ataaccgatc ctacccgctg
       61 tttttcccat catacaacat acaaatgccg tctgaaacat ccggcttcag acggcatttt
      121 ttcaaaaaac atttacaact caaaatcgcc caaatcatcc gtattcactt cagaatcgat
      181 ctgaccgatc aaataagagg atatttccac ttcctgcggc gcgacctgta cgttgtcgga
      241 cgacagccac gcattaatcc acggaatcgg gttttgattt gcgccttcaa atccggccgg
      301 caaccccacc gcctgcatac gcagattggt aatatattcg acgtattggg ataagatttc
      361 tttgttcaaa ccaatcatcg aaccgtcttt aaacaaatat gccgcccatt ctttttcctg
      421 ttccgccgct tttttgaaga gttggaaaca ttcgtcctgc aactcggcag caatttctgc
      481 catttcagaa tcatcaacac ccgaacgcat cagattaagc atatgctgcg tgccggtcag
      541 gtgcagggct tcgtcgcggg caatcagttt gatgattttg gcgttgcctt ccatcaactc
      601 gcgctcggca aaagcaaacg agcaggcgaa tgaaacgtag aaacggatgg cttccaacac
      661 gttgacgcac atcaggcaga gatagagttt tttcttcaac ccgcgcaaag acacagtaat
      721 gggttttccg ccgacattat gcatcccttc gcccaacagg ttgtaatact gggtgtattc
      781 gattaagtca tcgtaatagc aggcaatgtc ttcggcgcgg gcggtaatgt attcgttttc
      841 gacaatatca tcaaacacga ccgacggatc attcacaata ttgcggatga tgtgggtata
      901 gctgcgcgag tgtatggttt cgctgaagct ccacgtttca atccacgttt ccaactcggg
      961 aatcgaaacc aaaggcagca aggcaacatt cggactgcgc ccttggatgg aatcgagcag
     1021 tgtttgatat ttcagattgc tgatgaaaat atgtttttcg tgttcaggca ggttggcgta
     1081 gtcgatacgg tcgcgcgaca cgtcgatttc ttccggccgc caaaaaaacg acaattgttt
     1141 ttcaatcagt ttttcaaata cctcgtattt ctgctggtca taacgggcaa cattaaccgg
     1201 ctgaccaaaa aacatcggct cattcagcgc gtcgtttttg gttttgggaa aggtgctgta
     1261 tgacatagtt aagtgttcgc aggacataat ctattctcta tattaattaa aaatgatctg
     1321 taagttttaa ctaacctgct gagactttat atatttaata aaattttcta aagttaaaac
     1381 atgggttttc ccaaaagttc caaatgtttt tacggattct ttagggacaa ctaaggttaa
     1441 attctcatca ctcatttcct gaatttgagt atcagatata ccttcttgta gagtaaataa
     1501 atgcttatga ggaattcgat ctgcttcatt taaaacttgg cgccagcgat ccttacaagt
     1561 agtctttgaa cctagcattg tcagcttttc agtttgaaat atgaaattcc ctgattcatc
     1621 catcgcatgg tactcttcac ttccaggaaa taaaaaatct ggtttctttt tcccttctgt
     1681 cttcgcttgc gtctcaaatt ttaaggaaaa ttcgtcaaat attcttgata aatgtaactc
     1741 taaagatttt cctgctcttg cttttctgcg atttgtaaaa gaatgtgcaa aattaatgaa
     1801 gctatccaaa tcagtaattt ttgatttaat aatttcaaat tcacgttttt caaaaatgga
     1861 aaataattca tattcctttg aaacccattg ttttaacaca ttactaacgt tagttttatt
     1921 taatgcaata ttttcccttg ccaaaactgc catttcttca gttttgggga agttaggaaa
     1981 tagtgaaaat aattttgtta ggttatcctc tacttcttgt tttttaggta aatataaact
     2041 tcctggtaaa atgttagttt ctgcgataaa tatttcaata tcttcatctg aagataaaac
     2101 aaaagcatgg aataataaat ctttataaca agctctgcac aataccaata aggaaccact
     2161 atatttatca cttaaaaatt caaaattctt gccaaaaccc gtaattctcg cctcatttct
     2221 cgtgccttgg ccgtaatatt taaaattgct attagtgacg cttccatctt gccaattaat
     2281 tttgattgaa atagttttat ttgttccctt ttggcaaact acatcaaaga ggtcgtctga
     2341 aaaatgtttt tgaatataaa atcctgattg gtgactacca gtagtaccaa catcattgga
     2401 acgaatataa cgacagtata ctgcaattga tttatttgct gtctgtattg ctgaagctac
     2461 taagtcatcg gatttattta ctacttgtat tgtctgagct gccaagtcat tcatttttta
     2521 actcatttat aatttgttta gcaatagctt gaaccaacgg tacagcaatt gaattgccaa
     2581 actgcttgta tgcagctgtc ttggatactg catcaataac aaaatcttta ggaaatccca
     2641 ttaaacgcga gcactcccta ggtgtcagct tcctaggatt ttttcctttc tgagggatga
     2701 gtatttcgga accatctttg taatatcgtg cagatagagt tcgtgatatt ccatctaaat
     2761 caactaatcc aaaaccaaat ccattaccct ttgccttatg ttttttagcg taattttgaa
     2821 ggtaaagcca taagttatca gaaagagtaa aagaattatc tacatcatct tccaaaattt
     2881 gctttaattt tggttgtgat tctggtgggg aaggaaaatt gaaatttatt tccttattaa
     2941 aatattgtct atcaaaacct acaataaaaa tacgctccct attttgagga acataatatt
     3001 ttgcattcat aacttgataa aatatctgat agtcaagctc ttctaaagtc cctttaatta
     3061 ctttaaatgt atttcctttg tcatgcgaaa caaggttttt cacattctct aaaagaaaaa
     3121 ttttaggtcg atgttttcca ataatttcag caacatcaaa aaatagagtt ccctgcgcct
     3181 tatctaagaa gcctgtttct cgtcctaggc tttttttctt tgaaacacca gctatagaga
     3241 atggctgaca cgggaatcct gctgttaata catcaaactt acttggaata gctgctttgg
     3301 tttcctttaa tgtaatatct ccataaggaa tatcattaaa atttacttgg taggtttgac
     3361 gggctttatc atcccattca ctagaaaata cacatcgccc accaacattc tccattgcaa
     3421 tgcgaaaacc acctattccc gcaaataaat caataaaagt aaatttctca ttatttaagg
     3481 taactatatt atctaattga ttttgtattt tatttttcat attttatatt tcagatgatt
     3541 tattaatcta aaggccatct gaaaactccc cctttcatca aatcttacaa gccccgcccg
     3601 cgcagccgtc atcctgaata tcggtctgcg tatcgtccgc accgtcgcgg gtgttatggt
     3661 agtacagggt tttgacgccg tatttgtagg cggtcagcag gtctttgagc atttgtttca
     3721 tagaaacttt gccgccttcg aatttgcccg ggtcgtaggc ggtattggcg gaaatcgatt
     3781 gatcgacgaa tttttgcatc acgccgacaa gtttcaggta gccttcgttg ccgggaagct
     3841 gccacagggt ttcataggca tttttcaggg tttcaaactc cggcacgact tgtttcaaaa
     3901 tgccgtcttt cgatgctttg accgttacca atccgcgcgg cggctcgatg ccgttggtgg
     3961 cgttggcgat ttgcgaactg gtttcagacg gcatgagcgc ggtcagggtg gagttacgca
     4021 ggccgtattt gacgatgtcg gcacgcaggc tttcccagtc gtaatgcaaa ggctcgccgc
     4081 agacggcatc caaatctttt ttgtaagtgt cgatgggcag tttgccttgc gaataaacgg
     4141 tttggttaaa gagcgtgcac gcaccgtatt ctttggcaag gtttgccgat gctttgagca
     4201 ggtaatactg tatggcttca aaggtacggt gggtcagacc gagcgcggag ccgtcgctgt
     4261 agcggacacc gtttttcgcc agataataag catagttaat cacgccgatg ccgagcgaac
     4321 ggcgacccat agtagaggta cgcgcggctt ctaccggata tccctgataa tctaaaagtg
     4381 catcgagcgc acgcacggtc aagtcggcaa gcccttccaa ttcgtccaag ctgtttaatg
     4441 cgcccaagtt aaaggcagac agtgtacaca gggcgatttc gccgttcgga tcgttgatat
     4501 tgtccagcgg tttggtcggc agggcgattt ccatacacaa gttggactga tgaacaggcg
     4561 cgacgcacgg atcgaacggg ctgtgcgtat tgcagtgatc gacgttttga atgtagatgc
     4621 gcccggtccc ggcacgctcc tgcatcagcg tggaaaacag gtcggcagcc ggaatgatac
     4681 gcttgcgtat gttcgggtct tgctcgtatt tcgtatagag ccgctcaaat tcgtcttggt
     4741 cggcaaaaaa cgcttcgtac aaccccggaa cctcgttggg cgaaaacagc gtaatgttgc
     4801 cgcccttaat caggcgggtg tacagcaggc ggttgatttg cacgccgtaa tcaagctgac
     4861 ggatacggtt gtcttccaca ccgcggttgt ttttcaacac cagcaggctt tcggcttcga
     4921 tatgccacaa ggggtagaac aaagttgccg ctccgccgcg cacgccgcct tgcgaacagg
     4981 atttgaccgc cgcctgaaac attttgaaaa acggaatgca gccggtatgc cgcgcttcgc
     5041 cgccccggat ttcgctgtcc aaaccgcgga tacgtccggc attgatgccg atgcccgcac
     5101 gctgggaaac gtatttcaca atcgcgctgg tggtggcgtt gatggaatcc aggctgtcgt
     5161 cgcattcgat cagtacgcag cttgagaact ggcgcgtagg cgtacgcacg ccgctcataa
     5221 tcggggtcgg cagcgatact ttaaatgtgg aaacggcatc gtaaaaccgt ttgacgtaat
     5281 cgaggcgtgt ttctttggga tatttgctga aaagacacat cgccaccaga acatataaga
     5341 actgcggtgt ttcataaatc tggctggtaa cgcggttctg taccagatat ttgccttcga
     5401 gctgtttgac agcggcatag gaaaaggaca tatcgcgttc gtggtcgata taggcgttca
     5461 gttcatcaaa ttcttcgcgg ctgtaatcct caaggatatg cctgtcgtat tttccggcat
     5521 cggtgagctt tttaacgtgg tcgtaaaggt gcggcggctc gtattcgccg taggctattt
     5581 tacgcagatg gaaaatcgcc aaacgcgcgg caaggtattg gtagtccgga gtatcttccg
     5641 aaattaaatc ggcagcggcc ttgatgatgg tttcgtggat gtcgtcggtg cggatgccgt
     5701 tgtagaactg gatgtgcgat ttcaactcga cctgcgacac ggaaacattt tccaatcctt
     5761 ccgccgccca agtaacgaca cggtgaatct tatccaaatc aatggcttct aatcttccgt
     5821 ctcgtttggt tactttcaaa tcagtcggtg tattcatcgc ttcctcttcc actcttgata
     5881 ttcaagacac ggtcttttca aacaaattaa gacagacaat atagtggatt tttggcattt
     5941 tctccagtct tgacaacggt tgcatttttc agtttcaggc aatgcgcgat aaaacccgcc
     6001 tgtcgtattt ttcctataat atttgtttta tctgataatt ctttaccgat aaaaaacggg
     6061 taaatttttt gccttttgac cggctccggc tacaaggcgg tgaaataagg attttccgac
     6121 gaaaaaggaa agcttccggt tttctgcctc tgcgaattgt taaattttgc aaagtatgat
     6181 tttgccgcgc cgccgccgac aaattccatt ttcttaccga ttgaaattta ttattgagat
     6241 taatgtgtta tttgaatccg catatcaaac ggcaagtttt gtcggctgaa tgagtctgaa
     6301 actgccgaca atttgcccgt tccatccttc catcctatac tggaaggaat gacaaactga
     6361 aaggatcgtc atatctgtca aaaagatgct tgccatactg ttgtctgcaa tattgggact
     6421 ggtatcgaca actgccgctg ccggtacgtc agaacccgcc caccgccata ccaaacatat
     6481 ccgcaaggca aacaagcaga tgctgcaccc cgaatgcagg aaatatttgg aacgccgtgc
     6541 cgcgtggtac cgatcgcaag gcaacgtgca ggaattgcgc gaaaacaaaa aggcgcgcaa
     6601 agcattccgc tccctgcctt atgcggaaca gaaaatccaa tgccgggcgg cttatgaggc
     6661 tttcgatgat ttcgacggcg gcagtttccg ccgttaatcc catataa
//
LOCUS       DAWHSK010000098         6660 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.098, whole genome shotgun sequence.
ACCESSION   DAWHSK010000098 DAWHSK010000000
VERSION     DAWHSK010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6660
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.098"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..859)
                     /locus_tag="WIC03_001222"
     CDS             complement(<1..859)
                     /locus_tag="WIC03_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecG"
                     /protein_id="HEZ3883720.1"
                     /translation="MMSPETQKQLKITDVSAKKLDKLNLHTAWDLVLHLPLRYEDETH
                     IMPIKDAPIGVPCQVEGEVIHQEVTFKPRKQLIVQIADGSGSVLFLRFIHFYASHQKQ
                     TAVGKRIRAVGEIKHGFYGDEMIHPKIRDAEGGGLAESLTPVYPTVNGLNQPTLRRII
                     QTALDVTPLHDTLPDALLCRLKLPHLAESLRLLHSPPPSFTIHQLSDGTLPAWQRLKF
                     DELLAQQLSMRLARQKRIGGTAAALGGDGTLTQALRQALPFALTDAQEKVVSEICRDM
                     AQTYPMHRLL"
     gene            complement(946..1389)
                     /gene="secB"
                     /locus_tag="WIC03_001223"
     CDS             complement(946..1389)
                     /gene="secB"
                     /locus_tag="WIC03_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEZ3883721.1"
                     /translation="MSEELQPVFSIERLYVKDLSLEVPHAPQIFLEQGEPEVDMRVST
                     GSQKLEDGYYNVDVTVTVTAKLDNERTMFLNEVTQSGIFRLENIPEEDVQLLLGVACP
                     NILFPYAREAVSSTVTRAGFPPVLLAPINFEAIYQQQQEAEAAGA"
     gene            complement(1410..1667)
                     /gene="grxC"
                     /locus_tag="WIC03_001224"
     CDS             complement(1410..1667)
                     /gene="grxC"
                     /locus_tag="WIC03_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HEZ3883722.1"
                     /translation="MQTVTMYTGPFCPYCAMAKRLLHAAGVGHIDEIRVDASPEAFAE
                     MQQLSGQRSVPQIFIGETHVGGFTDLYRLQQEGGLDGLLNP"
     gene            1881..3374
                     /gene="rng"
                     /locus_tag="WIC03_001225"
     CDS             1881..3374
                     /gene="rng"
                     /locus_tag="WIC03_001225"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HEZ3883723.1"
                     /translation="MLSGLPIPKDIARPPETILVNITPQETRVAVLEENNICELHIER
                     NSEHSLVGNIYLGVVRRVLPGMQSAFIDIGLERAAFLHIVDVLEQRRNPEETQRIEHM
                     LFEGQSVLVQVIKDPINTKGARLSTQISLAGRFLVHLPQEDHIGVSQRIEDDAERSSL
                     RERLDKLLPENACRGYIIRTNAENATDEQLQSDIDYLTKVWEHIQEQAKIRPPETLLY
                     QDLPLSLRVLRDMVGCDTQKILVDSTVNHGRMTRFAEQYVHGALGRIELFKGERPLFE
                     THNVEQEISRALQPRVNLNFGSYLIIESTEAMTTIDVNTGGFVGARNFDETIFRTNLE
                     ACHTIARELRLRNLGGIIIIDFIDMAQESHREAVLQELAKALAFDRTRVTLHGFTSLG
                     LVELTRKRSRENLNQVLCEPCPSCQGRGRLKTPQTVCYEIQREIVREARRYDAESFRI
                     LAAPNVIDLFLDEESQSLAMLIDFIGKPISLAVETAYTQEQYDIVLM"
     gene            complement(3409..4659)
                     /locus_tag="WIC03_001226"
     CDS             complement(3409..4659)
                     /locus_tag="WIC03_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEZ3883724.1"
                     /translation="MFVPLAMLAGMFSYYETFHETEALQDDLLRQAALYVAPDSKPET
                     LPEGDGDTRILVQMPQQEDPVVSLPAHLADGLHTLQADGDDDYYRVYIRTTERGRIAV
                     MQESEYREDLAADAARQSVLPLLAALPLMILLTVWITHKAMRPVRKLSQSLEQRRIND
                     LSALSVDNIPSEIRGFVTAINLLLKRADEDIRHRQRFVADAAHELRTPMTALSLQAER
                     LNNMSLPPDAARQSAVLQQSIRRNKHLLEQLLALARSQSDETPLAKTTFGLQSRFRQV
                     LQELMPLALEKRQDIGVAVGGDVEIFADETEIYTLVKTFVDNAIRYTPSEGRIDLGFT
                     DEGKYLAVWVEDNGNGIPESERVRVLDPFYRILGTEQQGTGLGLSIADTLAKKYGGYL
                     ELTDSRRFGHGLLIRALLDKETLK"
     gene            complement(4672..4829)
                     /locus_tag="WIC03_001227"
                     /pseudo
     CDS             complement(4672..4829)
                     /locus_tag="WIC03_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692565.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
     gene            complement(5052..6365)
                     /locus_tag="WIC03_001228"
     CDS             complement(5052..6365)
                     /locus_tag="WIC03_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HEZ3883725.1"
                     /translation="MLTFIGLLIIGVIVWLLLTEKVSPIIALILVPLFGALLAGFDVS
                     QLKEFYSGGTKSVMQIVIMFMFSILFFGIMNDVGLFRPMIGGLIKLTRGNIVAVSVGT
                     VLVSVVAQLDGAGATTFLLVVPALLPLYKRLHMNPYLLFLLLTSSAGLINLLPWGGPT
                     GRVASVLGADVGELYKPLLTVQIIGVVFILALSLLLGVREKRRIVRELGALPAVADLI
                     KPVPLSEEEQKLARPKLFWWNVLLFLAAMSLLFSGIFPPGYVFMLAATAALLLNYRSP
                     QEQMERIYAHAGGAVMMASIILAAGTFLGILKGAGMLDAISKDIVHILPDALLPYLHI
                     AIGVLGIPLELVLSTDAYYFGLFPIVEQITSQAGVAPEAAGYAMLIGSIVGTFVTPLS
                     PALWMGLGLAKLSMGKHIRYSFFWAWGLSLAILASSIAAGIVPLP"
BASE COUNT         1619 a         1993 c         1682 g         1366 t
ORIGIN      
        1 gcagcaggcg gtgcatcggg taggtttgcg ccatatcgcg gcagatttcg gaaacaactt
       61 tttcttgcgc atcggtcagg gcaaacggca gggcttggcg cagggcttgg gtcaatgtgc
      121 cgtcgccgcc caatgccgcc gccgtgccgc cgatacgctt ctgtcgcgcc aagcgcatcg
      181 aaagctgttg cgccaaaagt tcatcgaatt tgagccgttg ccatgcaggc agcgtgccgt
      241 ctgaaagctg atgaatcgtg aaactcggcg gcggcgaatg caaaagacgc aggctttcgg
      301 cgaggtgtgg cagcttcaga cggcacagca gggcatcggg cagcgtgtcg tgcagcggcg
      361 taacgtccaa cgccgtctga ataatacggc gcaaagtggg ctggttcaaa ccgtttacgg
      421 tcgggtaaac cggcgtgagg ctttccgcca aaccgccgcc ctcggcatcg cggattttgg
      481 gatgaatcat ctcgtcgccg taaaagccgt gtttgatttc gcccacggcg cggatgcgct
      541 taccgaccgc cgtctgtttc tgatggctgg cgtaaaagtg gatgaagcgc agaaaaagga
      601 cgctgccgga accgtcggcg atttggacaa tcaactgctt gcgcggtttg aacgttactt
      661 cctgatggat aacctcgccc tcgacctggc acggcacgcc aatcggcgcg tccttaatcg
      721 gcataatgtg cgtctcgtcc tcgtaacgca gcggcaggtg caacaccaaa tcccacgcgg
      781 tatggaggtt gagtttgtcg agcttcttgg cggaaacatc ggtgattttg agctgtttct
      841 gggtttcggg cgacatcata ggcagattcc tttgaacgca cctattttat ccgaaaacaa
      901 aaatgccgtc tgaaacggat tcagacggca tcggacaggc aggaatcaag ccccggcggc
      961 ttcggcttcc tgctgttgtt ggtaaatcgc ctcaaaatta atcggcgcga gcaggacggg
     1021 cgggaagccg gcgcgcgtta ccgtactgga aacggcttcg cgcgcgtaag ggaagaggat
     1081 gttcggacac gccacgccca acagcagctg cacatcttct tcggggatgt tttccaaacg
     1141 gaaaatgccg ctttgggtta cttcgttcaa aaacatcgtg cgctcgttat ccaatttggc
     1201 ggtaacggtt acggtaacgt ccacgttgta gtagccgtct tccagctttt ggctgccggt
     1261 ggaaacgcgc atatccactt caggctcgcc ctgttccaaa aagatttgcg gcgcgtgcgg
     1321 cacttccaaa gacaagtctt tgacatacag tcgctcgatg ctgaatacgg gttgcagttc
     1381 ttcgctcatt ttgttttcct agttgggggt taagggttca gcagtccgtc cagcccgcct
     1441 tcctgctgga ggcggtagag gtcggtaaat ccgccgacgt gcgtttcgcc gatgaaaatc
     1501 tgcggcacgc tgcgctgtcc cgaaagctgc tgcatttcgg caaaggcttc ggggcttgca
     1561 tcgacacgga tttcgtcgat atgtccgaca cctgccgcgt gcagcagcct tttcgccatc
     1621 gcgcagtagg gacaaaacgg acctgtgtac atggtaacgg tctgcatatt gggtttccga
     1681 aagttttgca atgataatca atataggggc atttcccctg tttggcaagt gcggaacaga
     1741 tgcacgttca aacggcatgt gcggaatgtg tcaaagtttc ttttttaaag tatgataggc
     1801 attgtgaaaa atatttttgc acccgcgctg cgcggcggaa cgggtgcaaa aatattttta
     1861 ttacattttc aggaaaaacc atgttgtcag gactccccat ccccaaagac atcgcgcgcc
     1921 cgcccgaaac gatattggtc aacatcacgc cgcaggaaac gcgcgtagcg gtgttggagg
     1981 aaaacaatat ctgcgagctg cacatcgagc gcaacagcga acacagcctg gtcggcaata
     2041 tctatctggg cgtggtgcgc cgcgtgctgc ccgggatgca gagcgcgttt atcgacatcg
     2101 gcttggaacg cgcggcgttt ttacacatcg tcgatgtcct cgaacaacgc cgcaaccccg
     2161 aagaaaccca gcgcatcgaa catatgctgt ttgaagggca gtctgttttg gtgcaggtca
     2221 tcaaagaccc gatcaacacc aaaggcgcgc ggctttccac ccaaatctcg ctggcggggc
     2281 gtttcctcgt ccatcttccg caagaagacc acatcggcgt gtcccaacgc atcgaagacg
     2341 atgccgaacg cagcagcctg cgcgaacgcc tcgacaagct cctgccggaa aatgcctgcc
     2401 ggggctacat catccgcacc aacgccgaaa acgccaccga cgaacagctc cagtccgaca
     2461 tcgactacct gaccaaagtg tgggaacaca tccaagaaca ggcgaaaatc cggccgccgg
     2521 aaaccctgct ttatcaggat ttgcccttga gtttgcgcgt attgcgcgat atggtcggct
     2581 gcgacacgca aaaaatcctc gtcgattcca ccgtaaacca cgggcgcatg acgcgttttg
     2641 ccgaacaata cgtccacggc gcattgggca ggatagaatt gttcaaaggc gaacgcccgc
     2701 tgtttgaaac ccacaacgtc gaacaggaaa tcagccgcgc cctgcaaccg cgcgtcaacc
     2761 tcaacttcgg cagctacctg attatcgaat ccaccgaagc catgaccacg atagacgtga
     2821 acaccggcgg cttcgtcggc gcacgcaact tcgacgaaac catcttccgc accaacctcg
     2881 aagcctgcca caccatcgcc cgcgaattga ggctacgcaa cctcggcggc atcatcatca
     2941 tcgacttcat cgatatggcg caggaaagcc accgcgaagc cgtgttgcag gagcttgcca
     3001 aagccctcgc cttcgaccgt acccgcgtta ccctgcacgg ttttaccagc ctggggctgg
     3061 tcgagctgac gcgcaaacgc tcgcgcgaaa acttaaacca agtcctctgc gaaccctgcc
     3121 cttcctgcca aggcagaggc cgtctgaaaa cgccgcaaac cgtgtgctac gaaatccagc
     3181 gcgaaatcgt ccgcgaagcg cgccgttacg atgccgaaag tttccgcatc ctcgccgccc
     3241 ccaacgtcat cgatttgttt ttggacgaag aatcgcaatc cttggcaatg ctgatagatt
     3301 tcatcggcaa accgatttct ctggcggtcg aaaccgctta cacgcaggaa caatacgata
     3361 ttgtgctgat gtaaaaaatg ccgtctgaag ccttcagacg gcatctgtct atttcagggt
     3421 ttccttgtcc aacaaggcgc gtatcagcag accgtgtccg aacctgcggc tgtcggtcag
     3481 ttccaaatat ccgccgtatt ttttggctag cgtgtcggca atcgacagcc ccagccccgt
     3541 cccctgctgc tccgttccca aaatacggta aaacggatcg aggacgcgga cgcgttcgga
     3601 ttccggaatg ccgttcccgt tatcttccac ccacacggca agatatttcc cttcgtctgt
     3661 gaaacccaaa tctatcctgc cttcggacgg ggtataacgt attgcgttat caacaaacgt
     3721 tttaaccaag gtatagattt cagtttcatc ggcaaatatt tcgacatcac ctccgaccgc
     3781 cacaccaatg tcctgacgtt tttccaaggc aagcggcatc agttcctgca acacttggcg
     3841 gaaacggctt tgcagaccga atgtcgtttt cgccaaaggg gtttcgtccg actgcgaacg
     3901 cgccagtgcc aaaagctgct cgagcaggtg tttgttgcgc cgtatgcttt gctgcaaaac
     3961 ggcagactgc cgcgccgcat cgggtggcag agacatattg ttgagccgtt ccgcctgaag
     4021 ggaaagggct gtcatcggcg tacgcaattc gtgtgccgcg tcggcgacaa accgctgacg
     4081 gtggcggatg tcttcatccg cacgtttcaa aagcaagttg atggcggtta cgaaccctct
     4141 gatttcactg ggaatattgt ccacactcaa ggcagacagg tcattgattc ggcgttgttc
     4201 gagactttgc gacaatttgc ggacggggcg catggctttg tgcgtaatcc acacggtcag
     4261 cagtatcatc agcggcagtg ccgccagcag gggcaacacg ctttgccgtg ccgcatccgc
     4321 cgccaaatct tcccggtatt cgctttcctg catcacggca atccgccccc gctccgtcgt
     4381 gcggatatag acgcggtaat aatcgtcgtc cccgtccgcc tgaagcgtgt gcagaccgtc
     4441 cgccagatgc gcaggcaggc tgacaacagg gtcttcctgc tgcggcatct gtaccaaaat
     4501 acgcgtatcg ccgtcgccct cgggcaaagt ttcgggtttg gaatcggggg cgacgtacaa
     4561 tgccgcctga cggagcaggt cgtcctgcaa cgcttccgtt tcgtggaagg tttcgtagta
     4621 ggaaaacata cctgcaagca ttgccagcgg aacaaacatc caaaccgtcc gccccggcaa
     4681 acccaaatcc agcagcatca agtcataagg ctgggcagcg gcagccgcgc gccgtttttg
     4741 acccaatcca ccgcatagcc gccgtctttc aaacttgccg acaccgcctc cgcaatcatc
     4801 gcatcgtctt ccaccagcaa aacacgcatc aactttccct ttcagacaaa ccgtgcctat
     4861 tctaacaccc caaaattagc cgcaatttag cggcctttac gcttgccggt atttttcaaa
     4921 actgcagcac aaaaaaaccg cgccggcaac tgccttcaga cggcattggg gcgcgattgc
     4981 aacacacggg caggcaggca gagcctgcga cagaccacag gaacgattcg ggcttcagac
     5041 ggcttcgccg tttacggcag aggcacgatt cctgccgcta tcgaactggc caatatcgcc
     5101 agcgacaaac cccacgccca aaaaaacgaa taacggatgt gtttgcccat cgacaatttc
     5161 gccaaaccca agcccatcca caaagccggc gaaagcggcg taacaaaagt gccgacgata
     5221 ctgccgatca acatcgcata acctgctgct tcgggcgcca cgcccgcctg cgaggtaatc
     5281 tgctccacaa tcggaaacag tccgaaataa taagcgtccg tactcaaaac caactcaagc
     5341 ggaatgccca acacaccgat ggcaatatgc agataaggca gcagcgcgtc tggcaggata
     5401 tgcacaatgt ctttggaaat cgcgtccaac atccccgcac ccttcaaaat ccccaaaaac
     5461 gtacctgccg ccaaaataat ggacgccatc atcaccgcgc cgccggcgtg ggcataaatc
     5521 cgctccatct gttcctgcgg gctgcggtaa ttcaaaagca acgccgccgt tgcagccagc
     5581 ataaatacat aacccggcgg gaagatgccc gaaaaaagca ggctcatcgc cgccaaaaac
     5641 agcaggacat tccaccaaaa cagtttcgga cgcgccaatt tttgttcttc ttccgacaaa
     5701 ggcaccggct ttatcaaatc cgccacggcg ggcaacgcgc ccaactcccg gacaatccgc
     5761 cttttttcac gcacacccaa aagcagggac agcgcaagga taaacactac accgataatt
     5821 tgcaccgtca acaaaggttt atacaattcg cccacatctg cgcccaacac gcttgcaacc
     5881 cgcccagtcg gcccgcccca cggcagaagg ttaatcaatc ccgcactgga agtcagcagc
     5941 aaaaacagca ggtaaggatt catatgcaga cgcttgtaaa gcggcaaaag ggcggggacg
     6001 accaataaaa acgtcgtcgc acccgccccg tccaactgcg ccaccaccga caccaagacc
     6061 gtccccacac tcactgccac gatattaccc cgagtcagct taatcaaacc gcctatcatc
     6121 ggacggaaca gccccacatc gttcatgatt ccaaaaaaca aaatggaaaa cataaacata
     6181 atcacaatct gcatcaccga tttggtgccg cccgaataaa attcttttaa ttgggataca
     6241 tcaaaccccg ccagcaacgc cccaaacagc ggcaccaaga ttaatgcgat gatgggcgac
     6301 actttttccg tcagcagcaa ccatacgatg accccgataa tcagcagtcc gataaacgtc
     6361 agcatcattt ctcctttatt ttattttaaa cagaaaaccg accgtgcagg caaaaccgtc
     6421 cacagacgcg gaataagccc tgcattctac ttttttattt taaaataagt caatcaatca
     6481 tttcctccgc ctcccgtctg ccgtcattcc tgcatccgtc cgtcatttca cggcggcaac
     6541 cgatacggaa caaccggtaa atcggtatcg agacggcgcg ggggcattca tcccggtgcg
     6601 ccgattcaaa cgaaaccgcc cctatcattg cggagcgcgg ggcgtgccgt acacgcggga
//
LOCUS       DAWHSK010000099         6657 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.099, whole genome shotgun sequence.
ACCESSION   DAWHSK010000099 DAWHSK010000000
VERSION     DAWHSK010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6657
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.099"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..397)
                     /locus_tag="WIC03_001229"
     CDS             complement(<1..397)
                     /locus_tag="WIC03_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
                     /protein_id="HEZ3883726.1"
                     /translation="MMKKILAVSALCLMTAAARAADTYGYLAVWQNPQNADDVLQVKT
                     TKEDSTKSEAFSELEAFCKGQDTLAGIAEDEPTGCRSVVSLNNTCVALAYPKALGALR
                     VDNAVVITSPRFTSVHQVALNQCIKKYGVQ"
     gene            503..907
                     /locus_tag="WIC03_001230"
     CDS             503..907
                     /locus_tag="WIC03_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883727.1"
                     /translation="MANRFQQRKSAYRRLSGKPLFSDGIAANLCGGRKTLSYNLSRFK
                     ISIRSEMQKISFNLLKPANSPKIGKRRPGLNGAPHIPMPSPRYLPASPEMPGNNIHPA
                     KIRNNTPGGRQSQTAPQSIRHQKNKPPPRASS"
     gene            981..1727
                     /gene="pssA"
                     /locus_tag="WIC03_001231"
     CDS             981..1727
                     /gene="pssA"
                     /locus_tag="WIC03_001231"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HEZ3883728.1"
                     /translation="METPTNTPQRSLRQNSIYLLPNSFTIAALFSAFYAITQSMHGRY
                     ETAAIAVFISMLLDGMDGRVARLTNSQSAFGEQLDSLADMVSFGVAPALIAYKWQLWQ
                     FGKIGYSVAFIYCACAALRLALFNTLIGKVDKRWFIGVPSPTAAALIVGLIWVNHSVE
                     KFPAVHWWALGITLFAGLSMIVQIPFWSFKEINIRRQVPFVGMLLAVLLLLLVTWEPS
                     LVLFLFFLGYSLSGYIMAARRFWKKYRKAD"
     gene            1728..2534
                     /locus_tag="WIC03_001232"
     CDS             1728..2534
                     /locus_tag="WIC03_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEZ3883729.1"
                     /translation="MWHWDIILILLAVGSAAGFIAGLFGVGGGTLIVPVVLWVLDLQG
                     LAQHPYAQHLAVGTSFAVMVFTAFSSMLGQHKKQAVDWKTVFTMMPGMVFGVFAGALS
                     AKYIPAFGLQIFFILFLTAVAFKTLHTDPQTASRPLPGLPGLTAVSTLFGTMSSWVGI
                     GGGSLSVPFLIHCGFPAHKAIGTSSGLAWPIALSGAISYLLNGLNIAGLPEGSLGFLY
                     LPAVAVLSAATIAFAPLGVKTAHKLSSAKLKKSFGIMLLLIAGKMLYNLL"
     gene            complement(2727..3179)
                     /locus_tag="WIC03_001233"
     CDS             complement(2727..3179)
                     /locus_tag="WIC03_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEZ3883730.1"
                     /translation="MQIILLEKIGGLGNLGDIVTVKNGYARNFLIPAGKAKRATEANM
                     KEFEARRAELEAKQAEILADAQARQEKLDGQTITVAQKAGVDGRLFGSVTNADIAAAI
                     VATGIEAVKANVRLPNGPLKAVGEYEVEVALHTDAVAKITVAVVAATE"
     gene            complement(3196..3426)
                     /locus_tag="WIC03_001234"
     CDS             complement(3196..3426)
                     /locus_tag="WIC03_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEZ3883731.1"
                     /translation="MARQSFKRRKFCRFTAEKIQEVDYKQVDLLKDFISENGKIIPAR
                     ITGTKAFYQRQLAVAVKRARFLALLPYTDQHK"
     gene            complement(3433..3735)
                     /gene="priB"
                     /locus_tag="WIC03_001235"
     CDS             complement(3433..3735)
                     /gene="priB"
                     /locus_tag="WIC03_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HEZ3883732.1"
                     /translation="MGFNNLVSLAALIEKVFPIRYTPAGISVLDIILKHESWQEENGQ
                     QCLVQLEIPARILGRQAEEWQYRQGVYVHVEGFLAQKSRRSLMPMLRIQNIQEYKG"
     gene            complement(3736..4104)
                     /gene="rpsF"
                     /locus_tag="WIC03_001236"
     CDS             complement(3736..4104)
                     /gene="rpsF"
                     /locus_tag="WIC03_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEZ3883733.1"
                     /translation="MRHYEIVFIVHPDQSEQVPAMVERYKTMIAEANGKIHRLEDWGR
                     RQLAYPINKIHKAHYVLMNIETTPEVVEELETAFRFNDAVLRHLTIKTKHAVTEASPM
                     LGGEKAKNLLSGASEEAAAQ"
     gene            complement(4260..5210)
                     /gene="trxB"
                     /locus_tag="WIC03_001237"
     CDS             complement(4260..5210)
                     /gene="trxB"
                     /locus_tag="WIC03_001237"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEZ3883734.1"
                     /translation="MSQHRKLIILGSGPAGYTAAVYAARANLNPVIITGIAQGGQLMT
                     TTEVDNWPADADGVQGPELMARFLAHAERFGTEIIFDQINAVDLQKRPFTLKGDMGEY
                     TCDALIVATGASAKYLGLPSEEAFAGKGVSACATCDGFFYKNQDVAVVGGGNTAVEEA
                     LYLANIAKTVTLIHRRSEFRAEKIMIDKLMKRVEEGKIILKLESNLQEVLGDDRGVNG
                     ALLKNNDGSEQQIAVSGIFIAIGHKPNTDIFKGQLEMDEAGYLKTKGGTADNVGATNI
                     EGVWAAGDVKDHTYRQAITSAASGCQAALDAERWLGSQNI"
     gene            5349..>6657
                     /locus_tag="WIC03_001238"
     CDS             5349..>6657
                     /locus_tag="WIC03_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEZ3883735.1"
                     /translation="MQQKIRFQIEGMTCQACASRIEKVLNKKDFVESAGVNFASEEAQ
                     VVFDDSKTSVADIAKIIEKTGYGAKEKTEDTLPQPEAEHHIGWRLWLLFTINVPFLIG
                     MAGMMIGRHDWMIPPLWQFALASVVQLWLAIPFYKSAWASIKGGLANMDVLVTIGTVS
                     IYLYSVYMLFFSPHAAHGMAHVYFEAGVMVIGFVSLGKFLEHRTKKSSLNSLGLLLKL
                     TPTQVNVQRNGEWKQLPIDQVQIGDLIRANHGERIAADGIIESGSGWADESHLTGESN
                     PEEKKAGGKVLAGALMTEGSVVYRATQLGSQTQLGDMMNALSEAQGSKAPIARVADKA
                     AAVFVPAVVGIALLTFIVTWLIKGDWTVALMHAVAVLVIACPCALGLATPAAIMVGMD
                     KAVKHGIWFKDSAAMEEAAHVDAVVLDKTGTLTEGRPQVAAVYC"
BASE COUNT         1524 a         1799 c         1664 g         1670 t
ORIGIN      
        1 cctgtacgcc gtattttttg atgcactggt tgagtgcgac ctgatgaacg ctcgtaaaac
       61 gcggagaagt aatcacgacg gcgttgtcga cacgcagcgc gcccaaggct ttcgggtatg
      121 ccagcgcgac acaggtattg ttcagcgaca cgaccgaccg gcatccggtc ggctcgtctt
      181 cagcaatgcc cgcaagcgtg tcctgacctt tgcagaaggc ttccaactcg gaaaacgctt
      241 cgcttttcgt cgaatcttct tttgtggttt taacctgcaa aacatcgtcc gcattctgcg
      301 gattctgcca aacggcgaga tagccgtaag tatcggcagc ccgtgccgcc gcagtcatca
      361 ggcatagtgc cgatacggcc agtatctttt tcatcatgat aaattcccga cggttcgtcc
      421 aaattctgtt gcattataaa caaaaaacag gataagtccc gccttatcgg cttatccctc
      481 cccgcagatt gcaccgccgg gtatggcaaa ccgatttcag cagcgcaaat ccgcataccg
      541 ccgccttagc ggcaagccgt tgttttcaga cggcattgcg gccaaccttt gcggcgggcg
      601 aaaaaccttg tcctataatt tatcccgttt caaaatcagc atacggtcgg aaatgcaaaa
      661 aatatctttc aatttgttga agcctgcaaa ctccccgaaa atagggaaac gccgccccgg
      721 tttgaacggt gcgccgcata ttccgatgcc ctccccccga taccttccgg caagcccaga
      781 aatgcccggc aacaatatcc atccggcaaa aatccgaaac aacacacccg gtggcaggca
      841 gagtcaaacc gccccgcaaa gcatccgcca tcagaaaaac aaaccgcctc cgagggcttc
      901 atcctaaagg gcgtattgtt cgataatggt ttgggttata atcccctatc gattctccac
      961 gtccgtgaga cacttcagct atggaaaccc cgaccaacac cccgcaacgc tccctgcgtc
     1021 aaaacagtat ctacctgctg cccaactcct ttaccatcgc cgcgctgttt tccgcatttt
     1081 acgcaatcac ccaatccatg cacggacgtt atgaaaccgc cgccatcgcg gtattcatct
     1141 ctatgttgct ggacggtatg gacgggcgcg tggcgcggct gaccaacagc caaagcgcgt
     1201 tcggggagca gctcgacagc cttgccgata tggtcagctt cggcgttgct cccgctctga
     1261 ttgcctacaa atggcagctt tggcagttcg gcaaaatcgg ttattccgtc gccttcatct
     1321 actgcgcctg cgccgccctg cgccttgcgc tgttcaacac gctcatcggc aaggtggaca
     1381 aacgctggtt tatcggcgtg cccagtccga ctgccgccgc gctgattgtc gggctgattt
     1441 gggtcaacca cagcgtcgaa aaattccccg ccgtccactg gtgggcattg ggcatcacac
     1501 tgtttgccgg cctgtcgatg attgtccaaa tccctttttg gagttttaaa gaaatcaaca
     1561 tccgcagaca agtccccttt gtcggaatgc tgcttgccgt cttactgctg cttctggtca
     1621 cttgggaacc gtcgctcgtc ctcttcctgt tctttctcgg atacagcctg tccggctaca
     1681 ttatggcggc acgccgattc tggaaaaaat acagaaaggc ggattaaatg tggcattggg
     1741 acattatctt aatcctgctt gccgtaggca gtgcggcagg ttttattgcc ggcctgttcg
     1801 gcgtaggcgg cggcacgctg attgtccctg tcgttttatg ggtgcttgat ttgcagggtt
     1861 tggcacaaca tccttacgcg caacacctcg ccgtcggcac atccttcgcc gtcatggtct
     1921 tcaccgcctt ttccagtatg ctggggcagc acaaaaaaca ggcggtcgac tggaaaaccg
     1981 tatttacgat gatgccgggt atggtattcg gcgtattcgc tggcgcactc tccgcaaaat
     2041 atatcccagc gttcgggctt caaattttct tcatcctgtt tttaaccgcc gtcgcattca
     2101 aaacactgca taccgaccct cagacggcat cccgcccgct gcccggactg cccggactga
     2161 ctgcggtttc cacactgttc ggcacaatgt cgagctgggt cggcataggc ggcggttcac
     2221 tttccgtccc cttcttaatc cactgcggct tccccgccca taaagccatc ggcacatcat
     2281 ccggccttgc ctggccgatt gcactctccg gcgcaatatc gtatctgctc aacggcctga
     2341 atattgcagg attgcccgaa gggtcactgg gcttccttta cctgcccgcc gtcgccgtcc
     2401 tcagcgcggc aaccattgcc tttgccccgc tcggtgtcaa aaccgcccac aaactttctt
     2461 ctgccaaact caaaaaatcc ttcggcatta tgttgctttt gattgccgga aaaatgctgt
     2521 acaacctgct ttaaaacaca cgaaaaaacc tttttaccgt ttgcacaagt aattaatcag
     2581 gacaaagctg cccagtctcc tgttccgaca aaaggacaga caacctgacc gagacctttg
     2641 cagaatatac ggaaaacaaa acaaataccg tctgaaacca cattccgaca atcggcaggg
     2701 tttcagacgg catctgataa tttcaattac tcggttgcgg caacgacggc aacggtaatt
     2761 ttagcaacgg catcagtgtg caaagccact tccacttcgt actcgccaac ggctttcaga
     2821 ggaccgttcg gcagacgtac atttgctttc acggcttcga tgccagtcgc aacgattgca
     2881 gccgcaatat cagcattggt aacggaacca aacagacgac cgtccacacc ggctttttgt
     2941 gcaacggtaa tggtttggcc atccaatttt tcctgacggg cttgggcatc tgccaaaatt
     3001 tcggcctgtt tggcttccaa ttcggcacgg cgtgcttcaa actctttcat gtttgcttca
     3061 gtagcacgtt ttgccttacc agcaggaatc aagaagttgc gggcatagcc gtttttaacg
     3121 gttacgatgt cgcccaagtt gcccaaaccg ccgatttttt ctaacagaat aatttgcatg
     3181 attcaatctc caaaattatt tgtgttggtc ggtgtaaggc agcagagcca ggaagcgtgc
     3241 gcgttttacg gcaacagcca attggcgttg gtagaatgcc ttcgttcctg tgatgcgtgc
     3301 aggaatgatt ttaccgtttt cagagataaa gtctttcagc aaatcaactt gtttgtaatc
     3361 gacttcttgg attttttcag ccgtgaaacg gcagaatttt ctacgtttga atgattgacg
     3421 agccattgtc gtttaacctt tatattcttg aatattttgt atcctgagca tcggcataag
     3481 ggaacgtctg cttttttgag ctaaaaaacc ttcgacgtga acatatacac cttgccgata
     3541 ctgccactct tccgcctgcc tgcctaaaat ccgtgccgga atttccaatt ggacaaggca
     3601 ttgctgcccg ttttcctcct gccatgattc atgctttaaa ataatatcta agacagagat
     3661 tccggcaggc gtatatcgaa tagggaaaac cttttcaatc aacgcggcaa gcgaaacaag
     3721 attattgaat cccaattatt gggcagccgc ttcttcagac gcaccgctca gcaggttctt
     3781 agccttttca ccacccaaca taggggatgc ttcggtaacg gcgtgtttgg ttttgatggt
     3841 tagatggcgc aataccgcat cattgaaacg gaatgcggtt tccaactctt caaccacttc
     3901 gggagtggtt tcgatgttca tcaaaacgta atgggcttta tggattttgt taatcgggta
     3961 agccagttgg cggcgacccc aatcttccaa acggtgaatc ttaccgtttg cttcggcaat
     4021 catggttttg taacgttcaa ccatagcagg cacttgctcg ctttgatcag gatgaacgat
     4081 aaacacgatc tcgtaatgac gcatgttatc tccttatgga tggtaaaaac agccttctgc
     4141 catgcgaaag cagaaggcaa ggttcaaaga gcaggcatta tattggggtt tgccgacgga
     4201 atcaaggatt tggtgcgaaa aatttgcatt ccgccgaaaa tttcggtttc agacggcatt
     4261 caaatgtttt ggctgcccag ccagcgttcc gcgtccaaag ccgcctgaca gccggaagcc
     4321 gcgctggtaa tcgcctgacg gtaggtatgg tcttttacgt cgcccgccgc ccatacgcct
     4381 tcgatattgg ttgcgccgac attgtccgcc gtgccgcctt tggttttcag gtaaccagct
     4441 tcgtccattt ccaactgacc tttgaaaata tcggtattcg gcttgtgccc gatggcgata
     4501 aaaatgccgc tgacggcaat ttgttgctca gaaccgtcgt tgttttttaa taatgcgccg
     4561 tttacgcccc gatcgtcgcc cagtacttct tgcaggttgc tttccagctt gaggatgatt
     4621 ttgccctctt ccacgcgttt catcagtttg tcgatcatga ttttttcggc acggaactcg
     4681 ctgcggcggt ggatcagggt aacggttttg gcgatattgg caaggtagag tgcctcttca
     4741 actgccgtat tgccgccgcc aactacggca acatcttggt ttttgtagaa gaaaccgtcg
     4801 caggtggcac aagcggaaac gcctttccct gcaaacgctt cctcactcgg caaaccaagg
     4861 tatttggcgg acgcgcctgt tgcgacaatc agggcatcgc aagtgtactc gcccatatcg
     4921 cctttgagtg taaacgggcg tttttgcaga tcgacggcgt tgatttggtc aaaaatgatt
     4981 tccgttccga aacgttcggc gtgggcgaga aaccgcgcca tcaattccgg cccttgcacg
     5041 ccgtcggcat cggcaggcca gttgtccact tcggtggtgg tcatcagttg cccgccttgc
     5101 gcgatacctg taataatgac ggggtttaaa ttggcgcgcg cggcatagac agcggcggtg
     5161 tatccggcgg ggccggaacc caaaataatc agtttgcggt gttgggacat tgtttttcct
     5221 ttgctgtgtc aagttttcgg attctactcg aattatcggc gcgtttgaga aatttcgacc
     5281 atacgggcgt tcagacggca tcccgcaacc ttgactgccg tctgaatatc aaagcaggaa
     5341 tcacgcttat gcaacaaaaa atccgtttcc aaatcgaagg catgacctgt caggcatgtg
     5401 cttcgcgtat tgaaaaagtg ttgaacaaaa aagattttgt cgaatcggcg ggagtgaact
     5461 tcgccagcga ggaggcgcag gtagtgtttg acgacagcaa aacctcagta gccgacattg
     5521 ccaaaatcat tgagaaaacc ggttacggcg cgaaggaaaa aacggaagat acattgccgc
     5581 aacccgaagc agaacaccat atcggctggc ggctgtggct gctgttcacc atcaacgtcc
     5641 cgttccttat cggcatggcg gggatgatga tcggcagaca cgattggatg attccgccgt
     5701 tgtggcagtt cgcattggcg agtgtggtgc agctttggct ggcaatcccg ttttacaaaa
     5761 gcgcgtgggc gagtattaaa ggtggattgg cgaatatgga cgtgctcgtt accatcggca
     5821 cggtatcgat ttatctgtat tccgtctata tgttattttt cagtccgcat gcggcacacg
     5881 gcatggcgca tgtgtatttt gaagcaggcg tgatggtgat cggttttgtg tcgctgggca
     5941 aatttttgga acaccgtacc aaaaaatcca gcctgaacag cttgggcctg cttttgaaac
     6001 ttacaccaac ccaagtcaac gtgcaacgca acggcgaatg gaaacagctt cccatcgacc
     6061 aagtgcaaat cggcgacctt atccgcgcca accacggcga acgcattgcc gcagacggca
     6121 tcattgaaag cggcagcggt tgggcggacg agagccatct taccggcgaa tccaatcctg
     6181 aagaaaaaaa ggcgggcggc aaagtgttgg cgggcgcgtt aatgaccgaa ggcagtgtgg
     6241 tgtaccgcgc cacgcagctc ggcagccaaa cccagctcgg cgacatgatg aacgcgctct
     6301 ctgaagcaca aggcagtaaa gcaccgattg cgcgcgtagc cgataaagcg gctgcggtat
     6361 tcgtgcctgc cgtcgtgggc attgcgttgt tgacttttat tgttacttgg ctgattaagg
     6421 gcgattggac ggttgcgctg atgcacgccg tcgccgtttt ggtgattgcc tgcccgtgtg
     6481 cgctcggtct ggctacaccc gccgcgatta tggtcggcat ggataaagca gtgaaacacg
     6541 gcatttggtt taaagactca gcggcaatgg aagaagccgc ccacgtcgat gccgttgtgt
     6601 tggacaaaac tggcacgctg accgaaggca ggccgcaggt tgccgccgtt tattgtg
//
LOCUS       DAWHSK010000100         6590 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.100, whole genome shotgun sequence.
ACCESSION   DAWHSK010000100 DAWHSK010000000
VERSION     DAWHSK010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6590
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.100"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            75..704
                     /locus_tag="WIC03_001239"
     CDS             75..704
                     /locus_tag="WIC03_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEZ3883736.1"
                     /translation="MIQNVVTSIILYSGTAVDLLIILMLFFAKRKSRKDIINIYLGQF
                     LGSVSLILLSLLFAFVLDYIPSKEILGLLGLIPIFLGIKVLLLGDSDGEAIAKEGLRK
                     DNKNLIFLVAMITFASCGADNIGVFVPYFTTLNLANLIVALLTFLVMIYLLVFSAQKL
                     AQVPSVGETLEKYSRWFVAVVYLGLGVYILIENNSFDMLWTVSGQEKIL"
     gene            851..2527
                     /locus_tag="WIC03_001240"
     CDS             851..2527
                     /locus_tag="WIC03_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEZ3883737.1"
                     /translation="MLTYTPPDARPPAKTHEKPWLLLLMAFAWLWPGVFSHDLWNPAE
                     PAVYTAVEALAGSPTPLVAHLFGQTDFGIPPVYLWVAAAFKHLLSPWAADPYDAARFA
                     GVFFAVVGLTSCGFAGFNFLGRHHGRSVVLILIGCIGLIPVAHFLNPTAAAFAAAGLV
                     LHGYSLARRRVIAASFLLGTGWTLMSLAAAYPAAFALMLPLPVLMFFRPWQSRRLMLT
                     AVASLAFALPLMTVYPLLLAKTQPALFAQWLDDHVFGTFGGVRHVQTAFSLFYYLKNL
                     LWFALPALPLAVWTLCRTRLFSTDWGILGVVWMLAVLVLLAVNPQRFQDNLVWLLPPL
                     ALFGAAQLDSLRRGAAAFVNWFGIMAFGLFAVFLWTGFFAMNYGWPAKLAERAAYFSP
                     YYVPDIDPIPMAVAVLFTPLWLWAITRKNIRGRQAVTNWAAGVTLTWALLMTLFLPWL
                     DAAKSHAPVVRSMEASLSPELKRELSDGIECIDIGGGDLHTRIVWTQYGTLPHRVGDV
                     QCRYRIVRLPQNADAPQGWQTVWQGARPRNKDSKFALIRKIGENILKTTD"
     gene            2629..3234
                     /locus_tag="WIC03_001241"
     CDS             2629..3234
                     /locus_tag="WIC03_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEZ3883738.1"
                     /translation="MMTLYSGITCPFSHRCRFVLYEKGMDFEIKDVDIYNKPEDLAVM
                     NPYNQVPVLVERDLVLHESNIINEYIDERFPHPQLMPGDPVMRGRGRLVLYRMEKELF
                     NHVQVLENPAATNKEQAKAREAIGNGLTMLAPSFSKSKYILGEDFSMIDVALAPLLWR
                     LDHYDVKLGKSAAPLLKYAERIFQREAFIEALTPAEKAMRK"
     gene            3306..3698
                     /locus_tag="WIC03_001242"
     CDS             3306..3698
                     /locus_tag="WIC03_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HEZ3883739.1"
                     /translation="MPTSTKPYILRALCEWCSDNSLTPHILVWVNEHTRVPMQYVRDN
                     EIMLNIGATATQNLQIDNDWISFSARFGGQAHDIWIPVGHVLSLFARETGEGMGFELE
                     AYRPDTPPENTPAETAPRSAKKGLKLVK"
     gene            3703..3945
                     /locus_tag="WIC03_001243"
     CDS             3703..3945
                     /locus_tag="WIC03_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883740.1"
                     /translation="MPSERNRVSDGILSDGAQTESVYRQNPFRCIETVLPLPIRYWDV
                     IASALECNYGAANQAIERQRQRAAAVAVHERVAKFL"
     gene            complement(4023..4469)
                     /locus_tag="WIC03_001244"
     CDS             complement(4023..4469)
                     /locus_tag="WIC03_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEZ3883741.1"
                     /translation="MSLKIRLTEDMKTAMRAKDQVSLGTIRLINAAVKQFEVDERTEA
                     DDAKITAILTKMVKQRKDSAKIYTEAGRQDLADKENAEIEVLHRYLPQMLSAGEIRTA
                     VDAAVAETGAAGMADMGKVMGVLKTRLAGKADMGEVNKILKAVLTA"
     gene            complement(4601..4813)
                     /locus_tag="WIC03_001245"
     CDS             complement(4601..4813)
                     /locus_tag="WIC03_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEZ3883742.1"
                     /translation="MPAIRVKENEPFEVAMRRFKRAVEKTGLLTELRAREAYEKPTTE
                     RKRKKAAAVKRLQKRLRSQQLPPKMY"
     gene            complement(5102..>6590)
                     /locus_tag="WIC03_001246"
     CDS             complement(5102..>6590)
                     /locus_tag="WIC03_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEZ3883743.1"
                     /translation="AGRAQEVECYADSSRNDYTRAAELVKNTGKLPSGCTKLLEQAAA
                     SGLLDGNDAWRRVRGLLAGRQTTDARNLAAALGSPFDGGTQGSREYALLNVIGKEARK
                     SPNAAALLSEMESGLSLEQRSFAWGVLGHYQSQNLNVPAALDYYGKVADRRQLTDDQI
                     EWYARAALRARRWDELASVISHMPEKLQKSPTWLYWLARSRAATGNTQEAEKLYKQAA
                     ATGRNFYAVLAGEELGRKIDTRNNVPDADANSVLRMAEDGAIKRALVLFQNSRTAGDA
                     KMRRQAQAEWRFATRGFDEDKLLTAAQTAFDHGFYDMAVNSAERTDRKLNYTLRYISP
                     FKDTVIRHAQNVNVDPAWVYGLIRQESRFVIGAQSRVGAQGLMQVMPATAREIAGKIG
                     MDAAQFYTADGNIRMGTWYMADTKRRLQNNEVLATAGYNAGPGRARRWQADTPLEGAV
                     YAETIPFSETRDYVKKVMTNAAYYAALFGAPHIPLKQRMGIVPAR"
BASE COUNT         1319 a         1769 c         1787 g         1715 t
ORIGIN      
        1 acggggttga agttgacatc tacaaagttt aatgtttgaa tacattaaaa aaatatattt
       61 gaatgagatg ttttatgatt caaaatgttg ttacttcaat aatcctgtat tctgggacag
      121 ccgtagactt acttattatc ctaatgttat tttttgccaa aagaaaaagc agaaaagaca
      181 tcattaacat ctatttagga caatttctag gctctgttag cctgatattg ctaagtttgc
      241 tttttgcatt tgtcttagat tatattccta gtaaagagat tttaggtttg ctcggcttga
      301 ttccaatttt cctaggcatc aaagttttgc tgttaggaga ttctgatgga gaggctattg
      361 ccaaagaggg tttgcgcaaa gataataaaa acctgatttt tctagtcgct atgattactt
      421 ttgcaagttg tggtgctgac aatattggtg tctttgtccc atattttact accttaaatt
      481 tagcgaattt gatagtggct ttacttacct ttctagtcat gatttatctc ttggtttttt
      541 ctgcccaaaa attagcacaa gtcccttctg ttggagaaac tttggaaaaa tatagcagat
      601 ggtttgttgc tgttgtttat ttgggattgg gggtatatat cctgattgaa aacaacagtt
      661 ttgatatgct atggactgtg tcgggccagg aaaaaatatt atgaaaaaga tagtatctgc
      721 aaagactgcc atgtgcagtc ttttttgttg ccggtctttt tgtgtctgat gccgtctgaa
      781 gcagtctctg cacgaccttt gtgcgaatat ttgctacact gtggcaatcc gtccatttct
      841 tctcacgctt atgctgacct acactccgcc cgatgcccgc ccgcccgcca aaacccacga
      901 aaagccgtgg ctgctgctgt tgatggcgtt tgcctggttg tggcccggcg tgttttccca
      961 cgatttgtgg aatcctgccg aacctgccgt ctataccgcc gtcgaagcac tggcaggcag
     1021 ccccacccct ttggttgccc atctgttcgg tcaaaccgat ttcggcatac cgcccgtgta
     1081 tctttgggtt gccgccgcgt tcaaacattt gctgtcgccg tgggcagccg acccgtatga
     1141 tgccgcacgc tttgccggcg tgtttttcgc cgttgtcgga ctgacttcct gcggctttgc
     1201 cggtttcaac tttttgggca gacaccacgg gcgcagcgtc gtcctgattc tcatcggctg
     1261 tatcgggctg attccagttg cccatttcct caaccccact gccgccgcct ttgccgccgc
     1321 cggactggtg ctgcacggtt attctttggc tcgccggcgc gtgattgccg cctcttttct
     1381 gctcggtacg ggctggacgc tgatgtcgtt ggcagcagct tatccggcag catttgccct
     1441 gatgctgccc ttgcccgtac tgatgttttt ccgtccgtgg caaagtaggc gtttgatgtt
     1501 gacggcagtc gcctcacttg cctttgccct gccgcttatg accgtttacc cgctgctctt
     1561 ggcaaaaacg cagcccgcgc tgttcgcgca atggctcgac gatcacgttt tcggtacgtt
     1621 cggcggcgtg cggcacgttc agacggcatt cagtttgttt tactatctga aaaacctgct
     1681 ttggtttgca ttgcccgcgc tgccgctggc ggtttggacg ctttgccgta cgcgcctgtt
     1741 ttcgaccgac tgggggattt tgggcgttgt gtggatgctt gccgttttgg tgctgcttgc
     1801 cgtcaatccg cagcgttttc aggataacct cgtctggctg ctgccgccgc ttgccctgtt
     1861 cggcgcggcg caactggaca gcctgaggcg cggcgcggcg gcgtttgtca actggttcgg
     1921 cattatggcg ttcggactgt ttgccgtgtt cctgtggacg ggctttttcg ccatgaatta
     1981 cggctggccc gccaagcttg ccgaacgcgc cgcctacttc agcccgtatt acgttcccga
     2041 catcgatccc attccgatgg cggttgccgt actgttcaca cccttgtggc tgtgggcgat
     2101 tacccgcaaa aacatacgcg gcaggcaggc ggttaccaac tgggcggcag gcgttaccct
     2161 gacctgggct ttgctgatga cgctgttcct gccgtggctg gacgcggcga aaagccacgc
     2221 gcccgtcgtc cggagtatgg aggcatcgct ttccccggaa ttaaaacggg agctttcaga
     2281 cggcatcgag tgtatcgaca taggcggcgg cgacctacac acgcggattg tttggacgca
     2341 gtacggcaca ttgccgcacc gcgtcggcga tgtacaatgc cgctaccgca tcgtccgctt
     2401 gccccaaaat gcggatgcgc ctcaaggctg gcagacggtc tggcagggtg cgcgcccgcg
     2461 caacaaagac agtaagttcg cactgatacg gaaaatcggg gaaaatatat taaaaacaac
     2521 agattgagcc gaatttctgg attaagtgcc ggaaatcgcg tataattgcg cgattaaacc
     2581 tttataaatc agccgttttg taggcatcac acaggagcga caattattat gatgaccctc
     2641 tattccggca ttacctgccc cttcagccac cgctgccgtt tcgttttgta cgaaaaaggt
     2701 atggattttg aaatcaaaga cgtcgatatt tacaacaaac ccgaagacct cgccgtcatg
     2761 aacccgtata accaagtccc tgtgctggtc gaacgcgatt tggtgctgca cgagtccaac
     2821 atcatcaacg aatacatcga cgaacgcttc ccccatccgc agcttatgcc cggcgatccc
     2881 gttatgcgcg gcaggggccg gctggtgctg taccgtatgg aaaaagaatt gttcaaccac
     2941 gtccaagttt tggaaaaccc cgccgccacc aacaaggaac aggcaaaagc gcgcgaagcc
     3001 atcggcaacg gtctgaccat gcttgcccct tcgttcagca aaagcaaata catcctcggc
     3061 gaagattttt ctatgattga tgtcgccctt gctccgctgc tgtggcggct cgaccactac
     3121 gatgtcaaac tgggcaaaag tgccgcgccg ctgctcaaat acgccgagcg catcttccag
     3181 cgcgaagcct ttatcgaagc actgacaccc gccgaaaaag ccatgcgcaa ataagtccga
     3241 aatgcttgca aaacccgccg ttttgcaggc attttcacat tcaggcacac cacacggaac
     3301 ccatcatgcc cacctcaacc aaaccctaca tcctccgcgc actttgcgaa tggtgcagcg
     3361 acaacagcct cacaccgcac atccttgtct gggtcaacga acacacgcgc gtccccatgc
     3421 agtacgtccg cgacaacgaa attatgctca acatcggcgc gaccgccacg caaaaccttc
     3481 aaatcgacaa cgattggatc agcttttccg cccgcttcgg cggacaggcg cacgacatat
     3541 ggatacctgt cggacacgtc ctcagccttt tcgcacggga gaccggagaa ggtatggggt
     3601 ttgagttgga agcgtaccgc cccgacacgc cgcctgaaaa cacccctgcc gaaaccgcgc
     3661 cccggtccgc caaaaaaggc ttgaaattgg tcaaataaat ctatgccgtc tgaacggaat
     3721 cgtgtttcag acggcatttt gtccgatggg gcgcaaacgg aatccgttta tcggcaaaac
     3781 ccgtttcggt gtatcgaaac cgtgttgccc ctgccaattc gatattggga cgttattgca
     3841 tcagcattag agtgcaatta tggtgcagct aaccaagcga tagagcggca acgccaacga
     3901 gcagcggcag ttgctgtgca tgagcgtgtt gcaaaatttt tataaatatc atcaaaccat
     3961 gaaaatgccg tctgaacctg cgatatgggg ttcagacggc atttttcctt atattcatac
     4021 aatcaggcgg tcagcacggc tttcaggatt ttgttgactt cgcccatatc ggctttgccg
     4081 gcaagtcggg ttttcaatac gcccatcact ttgcccatat ccgccatacc tgccgcgccg
     4141 gtttcggcaa cggcggcatc gaccgcagta cggatttccc cggcggagag catttgggga
     4201 aggtagcggt gcagtacctc gatttcggcg ttttctttgt ctgccaaatc ctgacggccg
     4261 gcttcagtgt agattttcgc gctgtctttt cgctgtttga ccattttggt caggatggcg
     4321 gtgattttgg catcgtcggc ttcggtgcgt tcgtccactt caaactgttt gacggcggcg
     4381 ttgatgaggc ggatggtgcc gagggaaact tggtctttgg cgcgcatcgc ggttttcatg
     4441 tcttcggtaa ggcggatttt caggctcatg atgtcctcgc tgggatgtcg gatggaaacg
     4501 gcgggtttcg atgccgtctg aaaagcaaaa cacaccgcag agcaggtctt gcggtgtgtc
     4561 ctcatatcac agggctgacc tgtaatctgt acttgaatgt ttagtacatt ttgggcggca
     4621 gttgttggct gcgcaggcgt ttttgcaggc gttttacggc tgccgctttt ttgcgtttgc
     4681 gttcggtagt cggtttttcg taggcttcgc gggcgcgcag ctcggtcagc aggccggttt
     4741 tttctacggc gcgtttgaaa cggcgcatag cgacttcaaa gggttcgttt tcttttacgc
     4801 ggattgcagg cattttattt cctttaataa attctgttgt tgcatctgcc catcatatcg
     4861 gtggaaaggg tagggcagac ggtgttgaag gtttaccgta tcgctgtgcc ttgcggcggc
     4921 gggatacgcg ccgtgacgga aacatcacct cctaacgggt cggcttgtgc cgtgtcaagc
     4981 tgcttgaaaa agcaggaaaa acaattttcg gattgtctta tatttatggt ttgccgtcaa
     5041 tgccgttcgg gataaaaatg ccgtctgaaa gaccgggcgg gtttcagacg gcatcggtac
     5101 gtcagcgtgc aggaacaatg cccatacgct gtttgagcgg gatgtgcggc gcgccgaaga
     5161 gggcggcgta gtaggcggca ttggtcatca cttttttgac atagtcgcgc gtttcggaaa
     5221 acgggatggt ttcggcatat accgcgcctt cgaggggcgt gtccgcctgc catcggcgcg
     5281 ccctgccggg accggcgtta tagcctgcgg tggcgaggac ttcgttgttt tgcaggcggc
     5341 gtttggtgtc cgccatatac cacgtcccca tacggatatt gccgtcggcg gtgtaaaatt
     5401 gtgcggcatc cataccgatt ttgccggcga tttcgcgcgc ggtggcaggc ataacctgca
     5461 tcagcccctg tgcgcctacg cgggattgcg cgcctataac gaagcggctt tcctgacgaa
     5521 tcaggccgta aacccaagcc ggatcgacat taacattttg cgcgtggcgg attaccgtgt
     5581 ctttaaacgg cgaaatatag cgcaatgtgt agttgagttt gcggtcggtg cgttccgcgc
     5641 tgttgaccgc catatcgtaa aaaccgtggt cgaacgcggt ttgcgcggcg gtcagcagct
     5701 tgtcttcgtc gaacccgcgt gtggcaaaac gccattccgc ctgagcctga cggcgcattt
     5761 tcgcatcgcc ggcggtccgg ctgttttgga acagtaccag cgcacgctta atcgcgccgt
     5821 cttccgccat acggaggacg ctgtttgcgt cggcatcggg aacattgttg cgcgtatcga
     5881 ttttgcgccc caactcttcc cctgccagca ccgcataaaa attcctgccc gtcgctgccg
     5941 cctgtttgta aagtttttcc gcctcttgcg tgttgcccgt tgcggcgcgg ctgcgtgcca
     6001 gccagtagag ccaggtcggg cttttttgca gtttttcggg catatgcgag ataacggagg
     6061 ccagctcgtc ccaacgtcgg gcgcgcaagg cggcgcgggc gtaccactcg atttggtcgt
     6121 cggtcagttg gcggcggtcg gcaaccttgc cgtaatagtc caaggcggca ggcacattga
     6181 ggttttgcga ctgataatgc cccaatacgc cccacgcgaa actgcgttgt tcgaggctta
     6241 aaccgctttc catttcggac agcagggcgg cggcattcgg cgatttgcgt gcttctttgc
     6301 cgatgacgtt caacagggca tattcgcgcg aaccttgtgt accgccgtca aacgggctgc
     6361 ccaatgcggc ggcaaggttg cgtgcgtctg tggtttggcg gccggccagc agtccgcgca
     6421 cgcgcctcca ggcgtcgttg ccgtccaaca agccggatgc ggctgcctgt tccaacagtt
     6481 tggtgcagcc cgaaggcagt ttgcccgtat ttttgaccag ttcggcggca cgcgtatagt
     6541 cgttgcggct cgaatcggcg tagcattcga cttcttgggc gcgccctgcc
//
LOCUS       DAWHSK010000101         6584 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.101, whole genome shotgun sequence.
ACCESSION   DAWHSK010000101 DAWHSK010000000
VERSION     DAWHSK010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6584
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.101"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..140)
                     /locus_tag="WIC03_001247"
     CDS             complement(<1..140)
                     /locus_tag="WIC03_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEZ3883744.1"
                     /translation="MEFSTKTEILQEQQAGAQLFVCTEEAQLNHPTALALLSSLEEGQ
                     NFA"
     gene            310..1425
                     /gene="lptF"
                     /locus_tag="WIC03_001248"
     CDS             310..1425
                     /gene="lptF"
                     /locus_tag="WIC03_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HEZ3883745.1"
                     /translation="MIYQRNLIKELSFTAVGIFVVLLAVLVSTQAINLLGRAADGRVA
                     IDAVLALVGFWVIGMTPLLLVLTAFISTLTVLTRYWRDSEMSVWLSCGLALKQWIRPV
                     MQFAVPFAVLVAVMQLWVIPWAELRSREYAEILKQKQELSLVEAGGFNSLGKRNGRVY
                     FVETFDTESGIMKNLFLREQDKNGGDNIIFAKEGNFSLNDNKRTLELRHGYRYSGTPG
                     RADYNQVSFQKLNLIISTTPKLIDPVSHRRTIPTAQLIGSSNPQHQAELMWRISLTVS
                     VLLLCLLAVPLSYFNPRSGHTYNILIAIGLFLIYQNGLTLLFEAVEDGKIHFWLGLLP
                     MHIIMFVIAIVLLRVRSMPSQPFWQAVGKSLTLKGGK"
     gene            1422..2492
                     /gene="lptG"
                     /locus_tag="WIC03_001249"
     CDS             1422..2492
                     /gene="lptG"
                     /locus_tag="WIC03_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HEZ3883746.1"
                     /translation="MNLISRYIIRQMAVMAVYALLAFLALYSFFEILYETGNLGKGSY
                     GIWEMLGYTALKMPARAYELIPLAVLIGGLVSLSQLAAGSELTVIKASGMSTKKLLLI
                     LSQFGFIFAIATVALGEWVAPTLSQKAENIKAAAINGKISTGNTGLWLKEKNSIINVR
                     EMLPDHTLLGIKIWARNDKNELAEAVEADSAVLNSDGSWQLKNIRRSTLGEDKVEVSI
                     AAEENWPISVKRNLMDVLLVKPDQMSVGELTTYIRHLQNNSQNTRIYAIAWWRKLVYP
                     AAAWVMALVAFAFTPQTTRHGNMGLKLFGGICLGLLFHLAGRLFGFTSQLYGIPPFLA
                     GALPTIAFALLAVWLIRKQEKR"
     gene            2792..5377
                     /gene="acnB"
                     /locus_tag="WIC03_001250"
     CDS             2792..5377
                     /gene="acnB"
                     /locus_tag="WIC03_001250"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HEZ3883747.1"
                     /translation="MLEAYRKAAAERAALGIPALPLTAQQTADLVELLKSPPAGEGEF
                     LVELLAHRVPPGVDDAAKVKASFLAAVAEGSASSPLISPEYATELLGTMLGGYNIHAL
                     IELLDDDKLAPIAAKGLKHTLLMFDSFHDVQEKAEKGNKYAQEVLQSWADAEWFASRA
                     KVPEKITVTVFKVDGETNTDDLSPAPDAWSRPDIPLHALAMLKNPRDGITPDKPGEVG
                     PIKLLEELKAKGHPVAYVGDVVGTGSSRKSATNSVIWHTGEDIPFVPNKRFGGVCLGG
                     KIAPIFFNTQEDSGALPIEVDVSALKMGDVVDILPYEGKIVKNGETVAEFELKSQVLL
                     DEVQAGGRINLIIGRGLTAKAREALKLPASTAFRLPQAPAESKAGFTLAQKMVGRACG
                     LPEGQGVRPGTYCEPRMTTVGSQDTTGPMTRDELKDLACLGFSADMVMQSFCHTAAYP
                     KPVDVKTHKELPAFISTRGGVSLRPGDGVIHSWLNRLLLPDTVGTGGDSHTRFPIGIS
                     FPAGSGLVAFAAATGVMPLDMPESVLVRFSGKLQPGVTLRDLVNAIPLYAIKQGLLTV
                     AKAGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTVKLNKEPIIEYMKSN
                     VVLMKNMIANGYQDPRTLERRIKAMEKWLANPELLEADKDAEYAAVIEINMDDIKEPI
                     IACPNDPDDVCFMSERSGTKIDEVFIGSCMTNIGHFRAASKLLEGKADTPVRLWIAPP
                     TKMDAKQLSDEGHYGVLGRAGARMEMPGCSLCMGNQAQVREGATVMSTSTRNFPNRLG
                     KNTFVYLGSAELAAICSKLGKIPTVEEYQANIGIINEQGDKIYRYMNFNEIDSYNEVA
                     ETVNV"
     gene            5519..6514
                     /locus_tag="WIC03_001251"
     CDS             5519..6514
                     /locus_tag="WIC03_001251"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEZ3883748.1"
                     /translation="MNLKNRHFLKLLDFTPEEITAYLDLAAELKAAKKAGREIQRMKG
                     KNIALIFEKTSTRTRCAFEVAARDQGAGVTYLEPSASQIGHKESIKDTARVLGRMYDA
                     IEYRGFGQEVVEELAKYAGVPVFNGLTNEFHPTQMLADALTMREHSGKPLNQTAFAYV
                     GDARYNMGNSLLILGAKLGMDVRIGAPQSLWPSEGIIAAAHAAAKETGAKITLTENAH
                     EAVKNVDFIHTDVWVSMGEPKEVWQERIDLLKDYRVTPELMAASGNPQVKFMHCLPAF
                     HNRETKVGEWIYETFGLNGVEVTEEIFESPASIVFDQAENRMHTIKAVMVAALGD"
BASE COUNT         1603 a         1923 c         1640 g         1418 t
ORIGIN      
        1 gcaaaatttt gaccttcttc aagcgaagac aaaagggcaa gggcggtcgg atggtttaat
       61 tgcgcctctt cggtgcagac aaataactgt gcgcctgcct gctgttcctg caagatttcg
      121 gtttttgtgc taaattccac gtttattctc ctgattgaga cggttgtcgg tagttttcgg
      181 acggcctttc gctcaaaaga ccgtctgaag acggctggca cgattgtacc ccatttgaag
      241 caccgtctga aaccttgcgc ggacaatccg cctgcgccga accgcttacc gccccctgac
      301 cgcgattcta tgatttatca aagaaacctc atcaaagaac tctcttttac cgccgtcggc
      361 attttcgtcg tcctcttggc ggtattggtc tccacgcagg caatcaacct gctcggccgt
      421 gccgccgacg ggcgtgtcgc catcgatgcc gtgttggcat tggtcggctt ctgggtcatc
      481 ggtatgacgc cgcttttgct ggtgttgacc gcatttatca gtacattgac cgtgttgacc
      541 cgctactggc gcgacagcga aatgtcggtc tggctatcct gcggattggc attgaaacaa
      601 tggatacgcc cggtgatgca gtttgccgtg ccgtttgccg ttttggttgc cgtcatgcag
      661 ctttgggtga taccgtgggc agagctacgc agccgcgaat acgctgaaat cctgaagcag
      721 aagcaggaat tgtctttggt ggaggcaggc gggttcaaca gtttgggcaa gcgcaacggc
      781 agggtttatt ttgtcgaaac cttcgatacc gaatccggca tcatgaaaaa cctgttcctg
      841 cgcgaacagg acaaaaacgg cggcgacaac atcatcttcg ccaaagaagg taacttctcg
      901 ctgaacgaca acaaacgcac gctcgaattg cgccacggct accgttacag cggtacgcct
      961 ggacgcgccg actacaatca ggtttccttc caaaaactca acctgattat cagcaccacg
     1021 ccaaaactca tcgaccccgt ttcccaccgc cgtaccattc cgaccgccca actgattggc
     1081 agcagcaacc cgcaacatca ggcggaattg atgtggcgca tctcgctgac cgtcagcgtc
     1141 ctcctactct gcctgcttgc cgtgccgctt tcctatttca acccgcgcag cggacatacc
     1201 tacaatatct tgattgccat cggtttgttt ttaatttacc aaaacgggct gaccctgctt
     1261 tttgaagccg tggaagacgg caaaatccat ttttggctcg gactgctgcc tatgcacatc
     1321 atcatgttcg tcatcgcaat cgtacttctg cgcgtccgca gcatgcccag ccagcccttc
     1381 tggcaggcgg ttggcaaaag tctgacattg aaaggcggaa aatgaacctg atttcacgtt
     1441 acatcatccg tcaaatggcg gttatggcgg tttacgcgct ccttgccttc ctcgctttgt
     1501 acagcttttt tgaaatcctg tacgaaaccg gcaacctcgg caaaggcagt tacggcatat
     1561 gggaaatgct gggctacacc gccctcaaaa tgcccgcccg cgcctacgaa ctgattcccc
     1621 tcgccgtcct tatcggcgga ctggtctccc tcagccagct tgccgccggc agcgaactga
     1681 ccgtcatcaa agccagcggc atgagcacca aaaagctgct gttgattctg tcgcagttcg
     1741 gttttatttt tgctattgcc accgtcgcgc tcggcgaatg ggttgcgccc acactgagcc
     1801 aaaaagccga aaacatcaaa gccgccgcca tcaacggcaa aatcagcacc ggcaataccg
     1861 gcctttggct gaaagaaaaa aacagcatta tcaatgtgcg cgaaatgttg cccgaccata
     1921 cgcttttggg catcaaaatt tgggcgcgca acgataaaaa cgaattggca gaggcagtgg
     1981 aagccgattc cgccgttttg aacagcgacg gcagttggca gttgaaaaac atccgccgca
     2041 gcacgcttgg cgaagacaaa gtcgaggtct ctattgcggc tgaagaaaac tggccgattt
     2101 ccgtcaaacg caacctgatg gacgtattgc tcgtcaaacc cgaccaaatg tccgtcggcg
     2161 aactgaccac ctacatccgc cacctccaaa acaacagcca aaacacccga atctacgcca
     2221 tcgcatggtg gcgcaaattg gtttaccccg ccgcagcctg ggtgatggcg ctcgtcgcct
     2281 ttgcctttac cccgcaaacc acccgccacg gcaatatggg cttaaaactc ttcggcggca
     2341 tctgtctcgg attgctgttc caccttgccg gacggctctt cgggtttacc agccaactct
     2401 acggcatccc gcccttcctc gccggcgcac tacctaccat agccttcgcc ttgctcgccg
     2461 tttggctgat acgcaaacag gaaaaacgtt gaaccaatgc cgtctgcccc ccttcggacg
     2521 gcattggttt gtattatcat attcccgtag acagatagcc gttccctatt atagtggatt
     2581 aacaaaaacc agtacggcgt tgcctcgcct tgccgtacta ttgtactgtc tgcggcttcg
     2641 tcgccttgtc ctgatttaaa atacattacc tgtcataaca gttccatttt tgttaaaact
     2701 agtctatgat agcggtacaa atattgttta caatatttaa cgcaaatcat ttgcaacccg
     2761 acaaaagaaa aacagaaaaa ggaacaaaga gatgttagaa gcctatcgta aagccgccgc
     2821 cgagcgcgcc gcccttggta ttcccgccct tcctctgact gctcagcaaa ccgccgattt
     2881 ggttgagctg ctgaaaagcc cgcccgcagg cgaaggcgag ttcttggtcg aactgcttgc
     2941 ccaccgtgtt ccgcccggtg tggacgatgc cgccaaagtc aaagcctcat tcctggctgc
     3001 cgttgccgaa ggcagcgcgt ccagcccgct gatctccccc gaatatgcga ccgaactctt
     3061 aggtacgatg ctcggcggtt acaatatcca cgccttaatc gaactcttgg acgacgacaa
     3121 actcgcgccc attgccgcca aaggcttgaa acatacgctt ctgatgttcg attccttcca
     3181 cgacgttcag gaaaaagccg aaaaaggcaa caaatacgcg caagaagttt tgcaatcttg
     3241 ggcagatgcc gaatggttcg cctcacgcgc caaagttccc gaaaaaatca ccgttaccgt
     3301 tttcaaagtt gacggcgaaa ccaatacaga cgacctctcc cccgcgcccg acgcgtggag
     3361 tcgtcccgat attccgctgc acgcgctggc catgctgaaa aacccgcgcg acggcatcac
     3421 gcccgacaaa ccgggcgaag tcggtccgat taaattgttg gaagaactca aagccaaagg
     3481 ccatccggtt gcttacgtcg gcgacgtggt cggtactggt tcttcacgca aatccgcgac
     3541 caactccgtc atttggcata ccggcgaaga cattccgttc gtgccgaaca aacgcttcgg
     3601 cggcgtatgt ttgggcggca aaatcgcgcc gattttcttc aatacccaag aagattccgg
     3661 cgcgctgccg attgaagtcg atgtatctgc tctaaaaatg ggcgatgtcg tcgatatcct
     3721 gccttatgaa ggcaaaatcg tgaaaaacgg cgagactgtt gccgagtttg aattgaaatc
     3781 acaagtattg ctggacgaag tgcaagccgg cggccgtatc aacctgatta tcggtcgagg
     3841 tctgaccgcc aaagcgcgcg aagccctgaa actgcctgcc tctactgcat tccgcctgcc
     3901 gcaagcgcct gccgaaagca aagccggttt caccttggcg caaaaaatgg tcggccgcgc
     3961 ctgcggtctg cccgaaggac aaggcgtgcg cccgggtact tactgcgaac cgcgtatgac
     4021 gacggtcggc tcgcaagaca cgaccggccc gatgacccgc gacgagttga aagacttggc
     4081 ttgtttgggc ttctccgccg atatggtgat gcagtctttc tgccacaccg ccgcctatcc
     4141 gaaacctgtc gatgtaaaaa cccataaaga actgcccgcc tttatttcca cccgtggcgg
     4201 cgtgtcactg cgtccgggcg acggcgtcat ccactcgtgg ctcaaccgcc tgctgctgcc
     4261 cgataccgtc ggcaccggcg gcgacagcca tacccgtttc cccatcggta tttccttccc
     4321 cgccggctcc ggcttggttg cctttgccgc cgcaacgggc gtaatgccgc tcgatatgcc
     4381 cgagtctgta ttggtacgct tcagcggcaa gctgcaaccg ggcgtaaccc tgcgcgattt
     4441 ggtgaacgcc atcccgctgt acgcaatcaa acaaggtttg ctgaccgttg ccaaagccgg
     4501 taagaaaaac atcttctccg gccgcatcct cgaaatcgaa ggcctgcctg atttgaaagt
     4561 ggaacaagcc tttgaattga ccgacgcatc cgccgaacgc tccgccgccg gctgtaccgt
     4621 gaagctcaac aaagagccga ttatcgagta catgaaatcc aacgtcgtgt tgatgaaaaa
     4681 catgattgcc aacggctatc aagacccgcg cactttggaa cgccgcatca aagctatgga
     4741 aaaatggctg gcaaatcccg agttgctcga agcggataaa gatgccgaat acgccgccgt
     4801 gattgaaatc aacatggacg acatcaaaga gccgattatc gcctgcccga acgacccgga
     4861 cgacgtgtgc ttcatgtccg aacgctccgg caccaaaatc gacgaagtat tcatcggttc
     4921 gtgtatgacc aacatcggcc acttccgcgc cgcctccaaa cttttggaag gcaaggcaga
     4981 cacccccgtc cgcctgtgga ttgcgccgcc gaccaaaatg gacgcgaaac aattgtccga
     5041 cgaaggacac tacggcgtac tcggacgtgc cggcgcgcgt atggaaatgc cgggttgctc
     5101 cttatgtatg ggtaatcagg cgcaagtacg cgaaggtgcg accgttatgt ccacctccac
     5161 ccgcaacttc ccgaaccgtt tgggtaaaaa cacctttgtt tacctcggtt cggcggaatt
     5221 ggcagcgatt tgctccaaac tgggtaaaat cccgaccgtt gaagaatatc aagccaatat
     5281 cggcatcatc aacgaacagg gcgataaaat ctaccgctat atgaacttca acgaaatcga
     5341 cagctacaac gaagtagccg agaccgtgaa cgtttaatcc ccgtcatccg tatgaagtaa
     5401 gggattgacc gcaatgccgt ctgaacaacc ttcagacggc attgcaacat tccgctaacc
     5461 cttctttccg caaacgctgc aaatacggcg ttcacgcccc cacataaagg aaacgacagt
     5521 gaacctgaaa aaccgccatt ttctgaaact tttagacttc acgccggaag aaatcaccgc
     5581 ctacctcgac cttgccgccg aattgaaagc cgccaaaaaa gcagggcgcg agattcagcg
     5641 gatgaaaggg aaaaacatcg ccctgatttt tgaaaaaacc tctactcgga cgcgctgcgc
     5701 gtttgaagtc gccgcgcgcg atcaaggcgc gggagtgact tatttagagc cgtccgccag
     5761 ccaaatcggg cataaggaaa gcatcaaaga caccgcccgc gtgttgggca ggatgtacga
     5821 tgccatcgaa tatcgcggtt tcggtcagga agttgttgaa gaattggcga aatacgcggg
     5881 cgtacccgtg ttcaacgggc tgaccaacga gttccatccc acacaaatgc ttgccgacgc
     5941 actgactatg cgcgaacaca gcggcaaacc tttgaaccaa accgcgtttg cctacgtcgg
     6001 cgacgcgcgt tacaacatgg gcaattccct gctgatttta ggggcaaaat tggggatgga
     6061 cgtgcgtatc ggcgcaccgc aaagcctgtg gccgtctgaa ggcattattg ccgccgcaca
     6121 cgccgccgcc aaagaaaccg gcgcaaaaat taccctgacc gaaaacgcgc atgaagccgt
     6181 gaagaatgtt gattttattc ataccgatgt gtgggtcagc atgggcgagc cgaaagaagt
     6241 ctggcaggaa cgcatcgatt tgctgaaaga ttaccgcgtt acgcccgaac tgatggcggc
     6301 atcgggcaat ccgcaagtca aattcatgca ctgcctgccc gccttccaca accgcgaaac
     6361 caaagtcggc gaatggattt acgaaacctt cgggctgaac ggtgtggaag ttacagaaga
     6421 aatattcgaa agccccgcca gcatcgtgtt cgatcaggcg gaaaaccgta tgcacacgat
     6481 taaagcggta atggtcgcgg ctctgggcga ctgacagaac tgtgcctgtt taaattcatc
     6541 cgcaacacag ataccgtctg aacacgatgt tcagacggta tcca
//
LOCUS       DAWHSK010000102         6578 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.102, whole genome shotgun sequence.
ACCESSION   DAWHSK010000102 DAWHSK010000000
VERSION     DAWHSK010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6578
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.102"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            659..2107
                     /gene="gnd"
                     /locus_tag="WIC03_001252"
     CDS             659..2107
                     /gene="gnd"
                     /locus_tag="WIC03_001252"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019269816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HEZ3883749.1"
                     /translation="MKGDIGVIGLAVMGQNLILNMNDCGFKVVAYNRTIGKVDEFLNG
                     AAKGTDIVGAYSLQDLVDKLAKPRKIMMMVRAGSVVDDFIEQLLPLLEEGDIVIDGGN
                     ANYPDTTRRTHYLAEKGILFVGAGVSGGEEGARHGPSIMPGGDKRAWEAVKPIFQAIA
                     AKTPQGEPCCDWVGKDGAGHFVKMVHNGIEYGDMQLICEAYQFMKDGLGLSYDEMHRV
                     FAEWNKTELDSYLIEITAAILGYKDEGGEPLVEKILDTAGQKGTGKWTGINALDLGIP
                     LTLISEAVFARCVSSFKEQRVQTGKLFARTVTPVEGGKQEWVEALRQALLASKIISYA
                     QGFMLIREAGESYGWDLDYGNTALLWREGCIIRSAFLSNIRDAYENNPDLVFLGADPY
                     FKNILENCLPAWRKVVAKAVECGIPMPCMASAITFLDGYTTERLPANLLQAQRDYFGA
                     HTYERTDKPRGEFFHTNWTGKGGDTASTTYDI"
     gene            2170..3441
                     /gene="waaA"
                     /locus_tag="WIC03_001253"
     CDS             2170..3441
                     /gene="waaA"
                     /locus_tag="WIC03_001253"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HEZ3883750.1"
                     /translation="MFQWLYDVLWLLAPIWIRRYLDKRSGSAPAYRAHRDERFGKPYP
                     NPVTGAVWIHAVSVGETRAAQPLIRELRQRFPDAPLLMTQMTPTGRETAQVLFPDAQC
                     RYLPYDKKTWVRQFLREHRPMFGILMETEIWPNLMTECRKSGVPLFLANARLSEKSLN
                     GYLKVRRLIRPAAASLTGCLAQTEADAARLAKLGAASVQVCGNTKYDLMPSEQMKTLA
                     GQFEKRIGGRPVAVCGSTRVYRGEDEAEKLLAAWQQYRGDALLVVVPRHPEHFQTVFE
                     TAKRFGFKVQRRSDGLPVEPDTQVWIGDSMGELYAYYLCADVAFVGGSLVDSGCQNII
                     EPLSCGVPTIFGFSTYNFSEACRHALASGAAVRVESADAWREAVEKTLSSEGGGMQMQ
                     AHVDGFIAQHRGAGARIAEAVREAVCGHRGR"
     gene            3482..3931
                     /locus_tag="WIC03_001254"
     CDS             3482..3931
                     /locus_tag="WIC03_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family copper resistance protein"
                     /protein_id="HEZ3883751.1"
                     /translation="MSIYAVAHIVHLYCAIAFVGGVFFEVLVLSVLHTGRVSCEARRE
                     VEKAMSYRAVRVMPFVVGLLFASGVVMAANRYLSILGEPFATSFGTMLTLKILLAFSV
                     LAHFAIAVVKMARSTLTVGWSKYIHAVVFTHMLLIVFLAKAMFYISW"
     gene            3964..4938
                     /locus_tag="WIC03_001255"
     CDS             3964..4938
                     /locus_tag="WIC03_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEZ3883752.1"
                     /translation="MTEYPGIGSPLFYGVFFAAVLVMIALDMFSLKKNGSHKVGVKEA
                     LAWSGLWVAVSCLFAGWLYFELAGNLGYGAAVAKEKVLEFFTGYILEKSLAVDNIFVF
                     LMIFGYFKVAPQFQHRVLLYGVLGALVLRTVMIFVGAALVQQFEWILYLFGAFLLYTG
                     IHMMKPEGDEKEDLANSRLLNAVKKVVPVGTEFHGEKFFTVENGKKIATPLFLVLVMI
                     ELSDVVFAVDSIPAVFAVTTDPFIVLTSNIFAILGLRAMYFLLADVAERFIFLKYGLA
                     FVLSFIGVKMLVMHWVHIPISVSLSVVFGALGASILTSLIYTKKQPDK"
     gene            5007..5082
                     /locus_tag="WIC03_001256"
     tRNA            5007..5082
                     /locus_tag="WIC03_001256"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5040..5042,aa:Lys,seq:ttt)
     gene            5301..6554
                     /gene="murA"
                     /locus_tag="WIC03_001257"
     CDS             5301..6554
                     /gene="murA"
                     /locus_tag="WIC03_001257"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEZ3883753.1"
                     /translation="MDKLKISANGPLNGEITVSGAKNAALPLMCAGLLTSGTLRLKNV
                     PMLADVKTTQKLLQGMGARVLTDNISEFEINGGTVNNTCAPYELVRTMRASILVLGPT
                     LARFGEAQVSLPGGCAIGSRPVDQHLKGLEAMGAEIVIEHGYVKAKGKLKGTRVAMDV
                     VTVGGTENLLMAATLAEGTTVLENCAIEPEVVDLAECLVKMGAKISGIGTSTMIVEGV
                     DELHGCEHSVVPDRIEAGTFLCAVAITGGRVVLRNAAPKTMEVVLDKLVEAGAVIEAG
                     DDWIAIDMRQRPKAVDIRTVVHPGFPTDMQAQFMALNAVAEGSCRVVETIFENRFMHV
                     PELNRMGANITTEGNTAFVQGVEQLSGAVVKATDLRASASLVIAGLAARGETVVEQIY
                     HLDRGYENIEKKLGSVGAKIERVSG"
BASE COUNT         1517 a         1590 c         1906 g         1565 t
ORIGIN      
        1 ttcatgggaa tgacgggatg taggttcgtg ggaatgacgc ggtacaggtt ttcgttacgt
       61 atccccatcc gaaagtttat ttatacacaa gcaatccaaa gttatctgac tgaaatattg
      121 tgagatttac ccgcaaatct cattttttcc accacttata cacaaattcg gatgcgcact
      181 ctgccgattt gcagaaataa gacattcgtg ccgacacctg accatttcca cacgttccaa
      241 acacgcttcc ggcttaaatc tccacagtat tcccgttctt aaaggcgcca gcagccattc
      301 tgtaaagaaa agtaggatgc catctgcaca acaagcaaac tatccacaaa acttattccc
      361 cataaaaaac aatccacagg tatgcacaaa agggaatccg ccttaccgac accctaaaaa
      421 aaagaaatcc tgatgatttt taaaataaaa aaaagttacc cacagaaaat ttctcctatc
      481 aatataatca tcgttttatt atttaaattt tatattttat ttataaccct tcagcaaaac
      541 ggtacagcaa agcgtaaagg catcttttca aaaaattaaa aagttaagga ttgttaaaca
      601 agtgtaaatc gggtagagtt gcggtcgata aattaaatct atgacggagc ttcctacaat
      661 gaaaggcgat attggcgtaa tcggtttggc ggttatgggg caaaacctga ttttgaatat
      721 gaacgattgc ggatttaagg ttgtcgccta caaccgaaca atcggtaagg tggacgaatt
      781 tttaaacggc gcggctaagg gaacggatat tgtcggcgcg tattccctgc aagacttggt
      841 cgataagctg gcaaaaccga gaaaaatcat gatgatggtt cgcgcaggtt cggtggttga
      901 cgactttatc gaacaactgc ttccgttgtt ggaagaaggc gacattgtta tcgacggcgg
      961 taatgccaat taccccgaca cgacacggcg gacacattac cttgccgaaa aagggatttt
     1021 gtttgtcggg gcgggcgtat ccggcggcga agaaggcgcg cggcacggcc cgtccattat
     1081 gccgggcggg gataagcgcg cttgggaagc cgtcaaaccg atttttcagg cgattgccgc
     1141 caaaacgccg cagggcgaac cgtgttgcga ctgggtcggc aaggacggcg cggggcattt
     1201 tgtcaaaatg gtgcataacg gcatcgaata cggcgatatg cagttgattt gcgaagcgta
     1261 ccagtttatg aaagatggtt tgggattgtc ctacgatgaa atgcaccgcg tgtttgccga
     1321 gtggaacaaa accgagctgg attcttatct gattgaaatc acagcggcaa ttttgggcta
     1381 taaggacgaa ggcggtgaac cgctggtcga gaaaatcctc gatacggcag ggcaaaaagg
     1441 cacgggcaaa tggacgggca tcaatgcctt ggatttgggt attccgttga cgctgatttc
     1501 cgaagcggta ttcgcccgct gcgtttcgtc gttcaaagaa cagcgcgtgc agaccggcaa
     1561 gctgttcgcc cgaaccgtta cgcccgtcga aggcggcaaa caagaatggg ttgaggcgtt
     1621 gagacaggcc ctgcttgcat ccaaaatcat ttcctacgca caaggcttta tgctgatccg
     1681 cgaagcgggc gaaagctacg gctgggattt ggactacggc aacactgcgc tgctgtggcg
     1741 cgaggggtgc atcattcgca gcgcattttt aagcaatatc cgcgatgcct atgaaaacaa
     1801 tcccgatttg gtgttcttgg gtgcggatcc gtatttcaaa aatattttgg aaaactgcct
     1861 gccggcgtgg cgcaaggtgg ttgccaaggc ggtcgaatgc ggcatcccta tgccctgcat
     1921 ggcttcggcg attacgttct tggacggcta cacgaccgaa cgtctgcctg ccaacctctt
     1981 gcaggcacag cgcgactact tcggcgcgca cacttacgaa cgcaccgaca aaccgcgcgg
     2041 cgagtttttc cataccaact ggacgggcaa gggcggagat accgcttcga caacctacga
     2101 tatttgattc cgttgccgaa atgccgtccg aaccgttcgg acggcatttg ttttgcagga
     2161 aatagaaaga tgttccaatg gctttatgat gtattgtggc tgcttgcgcc gatatggata
     2221 cggcgttatt tggacaaacg ctccggaagt gccccggcat atcgggcgca tcgggacgag
     2281 cgtttcggca agccgtatcc gaatcccgtt accggagcgg tttggataca cgccgtttcg
     2341 gtcggagaaa cgcgtgccgc ccagcccttg atacgcgagt tgcggcagcg ttttcccgat
     2401 gcgccgctgc tgatgacgca gatgaccccg acgggacggg aaaccgcgca agttctgttt
     2461 cctgatgcac aatgccgtta tcttccgtat gacaaaaaaa cgtgggtacg gcagtttttg
     2521 cgcgaacacc gcccgatgtt cggcattttg atggaaaccg aaatctggcc caacctgatg
     2581 acggagtgcc ggaaatcggg cgttccgctg tttttggcga atgcgcggct gtcggaaaaa
     2641 tcgctaaacg gttatctgaa agtccgccgc ctgatccgtc ctgccgccgc ttcgctgacg
     2701 ggttgtctgg cgcagacgga ggcggacgcg gcgcggttgg cgaaattggg cgcggcatcc
     2761 gtgcaggtgt gcggcaatac caaatacgac ctaatgccgt cggaacagat gaaaacgctg
     2821 gcggggcagt ttgaaaaacg catcggaggc aggccggttg ccgtgtgcgg cagcacgcgc
     2881 gtttatcggg gtgaagacga ggcggaaaaa ctgctggcgg cgtggcaaca atatcgcggc
     2941 gatgcgctgc tggtcgtcgt gccgcgccat cccgagcatt ttcagacggt atttgaaacg
     3001 gcaaaacgct tcgggtttaa ggttcagcgg cgcagcgacg gtttgccggt cgaacctgat
     3061 acgcaggtgt ggataggcga cagtatgggc gagctgtatg cgtattacct gtgcgccgat
     3121 gtcgcttttg tcggcggcag tctggtcgat tcgggctgtc agaacattat cgaaccgctt
     3181 tcctgcggcg ttccgacgat attcggcttt tcaacctaca atttttccga agcctgccgg
     3241 cacgccttgg catcgggggc ggcggttcgg gttgagtcgg cggacgcgtg gcgggaagcc
     3301 gttgaaaaaa ccttatcgtc cgaggggggc ggaatgcaga tgcaggcgca cgtggacggc
     3361 tttatcgcac aacatcgcgg agcgggcgcg agaatcgccg aggcggtacg ggaagcggta
     3421 tgcggacatc ggggacgata gtgatacaat ccgtatccga gttttccggt tggagcatcg
     3481 tatgagtatt tatgccgtcg cgcacatcgt ccacctgtat tgcgccattg cctttgtcgg
     3541 cggcgtgttt tttgaagtgc tggttttgtc cgtcctgcat acgggacggg tgtcgtgcga
     3601 ggcgcggcgc gaagtggaaa aggcaatgtc ttaccgcgcc gtcagggtga tgccgtttgt
     3661 ggtcggactg ctgttcgcca gcggcgtcgt gatggcggca aaccgctatc tttctatatt
     3721 gggcgaaccg tttgccactt ccttcggtac gatgctgacg ctgaaaatcc tgttggcgtt
     3781 cagcgtgttg gcgcacttcg ccatcgccgt cgtcaaaatg gcgcgttcca cactgaccgt
     3841 cggctggtcg aaatacatac acgccgtcgt ctttacccat atgctgctga ttgtcttttt
     3901 ggcaaaagcg atgttttata tcagctggta acacaattac atttcaaatc aggagattga
     3961 accatgacag aatatcccgg catcgggtcg ccgttgtttt acggcgtttt ttttgcggca
     4021 gtgctggtca tgattgcctt ggatatgttt tcgctgaaga aaaacggcag ccataaagtc
     4081 ggcgtcaaag aagccttggc atggagcggc ttgtgggttg ccgtatcctg cctgttcgcg
     4141 ggctggctgt atttcgaact cgccggcaat ctcggctacg gcgcggcagt cgcaaaagaa
     4201 aaagtattgg aattctttac cggctacatt ttggaaaaat cgttggcagt cgacaatatt
     4261 ttcgtgttcc tgatgatatt cggctacttc aaagtcgcac cacagtttca gcaccgcgtg
     4321 ctgctgtacg gcgtattggg cgcattggta ttgcgcactg tcatgatttt cgtcggcgcg
     4381 gcactggttc agcagtttga gtggattctg tatctgtttg gcgcgttcct gctctatact
     4441 ggtatacaca tgatgaagcc cgaaggcgat gagaaagagg atttggcaaa cagcaggctg
     4501 ctgaatgccg tcaagaaagt cgttccggtc ggcacggaat ttcacggcga gaaatttttt
     4561 accgtcgaaa acggcaaaaa aatcgccacg ccgctgtttt tggtgctggt catgattgaa
     4621 ttgagcgatg tcgtgtttgc cgtggacagt attcccgccg tctttgctgt taccaccgat
     4681 ccgtttatcg tgctgacctc caatattttc gctattttgg gtttgcgggc gatgtatttc
     4741 ctgctggcgg atgtggcgga acgctttatc ttcctgaaat acggcttggc attcgtgttg
     4801 agttttatcg gtgtgaaaat gctggtaatg cattgggtgc atatcccgat ttccgtttcg
     4861 ctgtcggtcg tgttcggcgc gttgggtgca tcgatactga cctcgttaat ttatacgaaa
     4921 aaacagcctg ataaataaaa tgccgtctca acgccgacaa aatggttgac agcatcaggc
     4981 ttgatgtata gaatttcgag cttgtcgggt cgttagctca gccggtagag cagcggactt
     5041 ttaatccgtt ggtcgagcgt tcgaatcgct cacgacccac cagataaaac aaaaaaagct
     5101 gagttttcaa aactcggctt ttttgttttc cgacaatcct tgttcggaaa gcgcggcatt
     5161 tcgtcaaatt ctttggtgcg gcttccgtat ttttcccgaa aatcccttat aattccgcct
     5221 ctgcaacctt catcgggtga tgctttatgc gttgtcccga gaattgaaat attacaagta
     5281 cccaccagga aagaacaaac gtggacaaac tgaaaatctc cgcaaacggc ccgctcaacg
     5341 gggaaataac ggtctcgggc gcgaaaaacg cggcattgcc gctgatgtgc gcgggtttgc
     5401 tgacatcggg tacgttgcgc ctgaaaaacg tccctatgct ggcagatgtc aaaaccacgc
     5461 aaaaactgct tcaggggatg ggcgcgcgcg tcctgaccga caatatcagc gaatttgaaa
     5521 tcaacggcgg tacggtcaac aacacgtgcg ccccttacga gttggttcgg acgatgcgcg
     5581 cttcgatttt ggtgctgggc ccgacgctgg cgcgtttcgg cgaggcgcaa gtcagcctgc
     5641 cgggcggctg cgccatcggt tcgcgccccg tcgatcagca tttgaaaggc ttggaagcga
     5701 tgggggcgga aatcgtcatc gaacacggtt acgtcaaggc taagggtaaa ctcaaaggta
     5761 cgcgcgtggc gatggatgtc gttaccgtcg gcggcacgga aaacctgctg atggcggcga
     5821 cgctggcgga aggtacgacg gttttggaaa actgcgccat cgagcctgaa gtggtcgatt
     5881 tggcggaatg cctggttaaa atgggcgcga aaatcagcgg catcggaaca tcgaccatga
     5941 tcgtggaagg tgtggacgag ctgcacggct gcgaacacag cgtcgtcccc gaccggatcg
     6001 aggcggggac gttcctgtgc gcggtggcga taaccggcgg cagggtggtt ttgcggaatg
     6061 ccgccccgaa aacgatggaa gtggtgttgg acaaactggt tgaggcaggt gcggtgattg
     6121 aggcgggcga cgattggatt gccatcgata tgcggcagcg tccgaaggcg gtggacatcc
     6181 gcacggtcgt ccaccccggc ttccccacgg atatgcaggc gcagtttatg gcattgaatg
     6241 ccgtggcaga gggaagctgc cgcgtggtgg aaacgatttt tgaaaaccgc tttatgcacg
     6301 tccccgagtt gaaccggatg ggggcgaaca tcacaaccga gggcaatacg gcatttgtgc
     6361 agggtgtgga acagctttcc ggcgcggtcg ttaaggcgac ggatttgcgt gcgtccgcaa
     6421 gcctcgttat cgccggtttg gcggcgcgcg gcgaaaccgt ggtcgaacag atttaccact
     6481 tggatcgcgg ttatgaaaat attgaaaaaa aactcggcag cgtcggcgcg aaaatcgagc
     6541 gcgtatccgg ctgatcgggt tgcgatgccg tctgaagc
//
LOCUS       DAWHSK010000103         6556 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.103, whole genome shotgun sequence.
ACCESSION   DAWHSK010000103 DAWHSK010000000
VERSION     DAWHSK010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6556
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.103"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(119..1336)
                     /locus_tag="WIC03_001258"
     CDS             complement(119..1336)
                     /locus_tag="WIC03_001258"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEZ3883754.1"
                     /translation="MALIVHKYGGTSVGSPERIKNVAKRVAKARAEGHDIVVVVSAMS
                     GETNRLVALAHEMQEHPDPRELDVVLSTGEQVTIGLLAMALKDIGVDAKSYTGWQVAL
                     KTDTAHTKARIESIDDEKMRADLDAGKVVIVAGFQGISSEGDISTLGRGGSDTSAVAL
                     AAALKADECQIYTDVDGVYTTDPRVVPEARRMDTVTFEEMIELASLGSKVLQIRSVEF
                     AGKYKVRLRVLSSLQDGGNGTLITFEEDDNMERAAVTGIAFDKNQARINVRGVPDKPG
                     VAYQILGAVADANIEVDMIIQNVGSEGTTDFSFTVPRGDYKQTLEILSERQDSIGAAS
                     IDGDDTVCKVSAVGLGMRSHVGVAAKIFRTLAEEGINIQMISTSEIKVSVLIDEKYME
                     LATRVLHKAFNLG"
     gene            complement(1538..2266)
                     /gene="rph"
                     /locus_tag="WIC03_001259"
     CDS             complement(1538..2266)
                     /gene="rph"
                     /locus_tag="WIC03_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEZ3883755.1"
                     /translation="MPDYIRISRAADSLRDIKITPHFLPHTDGSCLIECGNTKVICTA
                     SIDENVPPFLRGKNQGWVTAEYGMLPASTASRMRREASAGKQSGRTQEIQRLIGRSLR
                     AVVDMEKLGERQILIDCDVIQADGGTRTASITGAFVALQIAVGKLVSDGILSENPIRE
                     AVAAVSVGVVNGVPLLDLDYPEDSGCDSDVNIVMTASGKIIEIQGTAEDAPFSLDELG
                     KLVALAQKGIGELLQHQQNALSAA"
     gene            complement(2348..2812)
                     /locus_tag="WIC03_001260"
     CDS             complement(2348..2812)
                     /locus_tag="WIC03_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEZ3883756.1"
                     /translation="MYKHLVVAVDGSETSINALKHAAELAGVNGARLTLVHVANPAEY
                     MALAPEFLQHESYEAAAVAQGNEVLDAAERTARELGVENIVKHLLVANKGAREMAQDL
                     VDYADENGADLLVLGTHGRTGLMHLLMGSFAETVMRQSHLPLLIIRSKAEEA"
     gene            3029..3805
                     /locus_tag="WIC03_001261"
     CDS             3029..3805
                     /locus_tag="WIC03_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HEZ3883757.1"
                     /translation="MSDNALTSSRRFGGIARLYGDSALAHFSQAHVCVVGVGGVGSWA
                     VEALARTGIGRLTLIDLDNVAESNVNRQLHALTGDFGKAKVTALRERVAQINPECQVF
                     EIEDFVTEDNLPEYFGKGFDFVIDAIDQVRVKAAMAAYFVERKQPFVLSGGAGGQKNP
                     ALIQTADLSRVTHDPLLANLRYTLRKRYGFSRDTKANMRVPCVYSTENIVPPQSREAC
                     SADAAPQGLSCAGYGASMLVTASFGLYCAQAAVEHIADKK"
     gene            4050..4904
                     /locus_tag="WIC03_001262"
     CDS             4050..4904
                     /locus_tag="WIC03_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEZ3883758.1"
                     /translation="MPPIPAPSAPSEHTVAWVFGQPVTDLPQDLFIPPDALKVVLGSF
                     QGPLDLLLYLIRKQNIDVLDIPMVKITEQYLHYIAQIEAYQFDLAAEYLLMAAMLIEI
                     KSRLLLPRTETVEDEEADPRAELVRRLLAYEQMKLAAQGLDALPRAGRDFAWAYLPLE
                     IAVEAKLPEVYITDLTQAWLGILSRAKHTRSHEVIKETISVRAQMTAILRRLNGHGIC
                     RFHDLFNPEQGAAYVVVNFIALLELAKEGLVGIVQEVGFGEIRISLNHEGAHSDGISG
                     TRGGRDVF"
     gene            4945..6153
                     /gene="pncB"
                     /locus_tag="WIC03_001263"
     CDS             4945..6153
                     /gene="pncB"
                     /locus_tag="WIC03_001263"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEZ3883759.1"
                     /translation="MTGIIHSLLDTDLYKFTMLQVVLHQFPQTHSLYEFRCRNASTVY
                     PLADIREDLEAELDALCQLRFTHDELDYLRSLRFIKSDFVDYLELFQLQRRFVEIGTD
                     DKDRLNIRIEGPMIQAMFFEIFILAIVNELYFRRLETPAVIEEGERRLQAKAARLKEI
                     AAAQNPDEPPFLISDFGTRRRYKLAWQEHVIRTLLEAAPSIVRGTSNVFLAKKLGITP
                     IGTMAHEFLQAFQALDVRLRNFQKAALESWVHEYRGDLGVALTDVVGMDAFLRDFDLY
                     FAKLFDGLRHDSGDPYVWGDKAYAHYQKLKIDSRTKMLTFSDGLDIERSWALHQYFKD
                     RFKTGFGIGTNLTNDMGHTPLNIVLKLVECNGQSVAKLSDSPGKTMTNNSTFLAYLRQ
                     VFDVPEPETP"
     gene            6245..>6556
                     /locus_tag="WIC03_001264"
     CDS             6245..>6556
                     /locus_tag="WIC03_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEZ3883760.1"
                     /translation="MNLHQTVEHEAAAAFAAAGIAGSPVVLQPTKNAEHGDFQINGVM
                     GAAKKAKQNPRELAQKVADALAGNAVIESAEVAGPGFINLRLRPEFLAQNIQTALNDA
                     RF"
BASE COUNT         1495 a         1890 c         1671 g         1500 t
ORIGIN      
        1 ttaatccact atattttttg ttccaaagtc aaaatatgcc gtccgaacat tcgggcggca
       61 gacaaaacgg cactgcccga taaaggcagt gccgttgtcc gtttcaaacc gtgaaacatc
      121 agcccaaatt aaaggcttta tgcaataccc tggttgccag ttccatgtat ttttcatcaa
      181 tcaatacgga aactttgatt tcggaggtgg aaatcatttg gatgttgata ccctcttcgg
      241 cgagcgtgcg gaagattttg gcggctacac cgacgtgcga acgcataccc aaaccgactg
      301 cggagacttt gcacacggtg tcgtcgccat caatagaagc cgcgccgata ctgtcttggc
      361 gttccgacag gatttccaaa gtctgcttgt aatcgccgcg cggtacggta aaggaaaaat
      421 cggttgtgcc ttcgctgccg acattttgga taatcatatc gacttcgatg ttggcatcgg
      481 cgaccgcgcc caaaatctga taggcgacac cgggtttgtc gggtacgccg cgcacgttga
      541 tgcgggcttg gtttttgtcg aatgcgatac cggttacggc agctctttcc atgttgtcgt
      601 cctcttcaaa ggtaattaag gtgccgttgc cgccgtcttg caggctgctc agtacgcgca
      661 ggcgcacttt gtattttccg gcaaattcta ctgaacggat ttgcaaaact ttcgaaccga
      721 ggcttgccag ttcgatcatt tcttcaaatg taaccgtatc catgcggcgc gcttcgggta
      781 cgacgcgggg gtcggttgtg taaacgccgt ctacgtcggt atagatttgg cattcgtccg
      841 ctttgagggc tgcagcaagc gcaacggcgg aagtgtcgga accgccgcgt ccgagcgtgg
      901 aaatatcgcc ttcactgctg atgccttgga agccggcaac gatgacgact ttgcctgcat
      961 cgaggtcggc acgcattttt tcgtcatcaa tgctttcgat gcgggctttg gtgtgggcgg
     1021 tatcggtttt gagggcgacc tgccagcctg tgtagctttt ggcatccacg ccgatgtctt
     1081 tcaatgccat cgccaaaagg ccgatggtta cttgttcgcc ggtggacaaa accacgtcca
     1141 attcgcgcgg atcgggatgc tcctgcattt cgtgcgccaa cgcgaccagg cggttggttt
     1201 caccgctcat ggcggatacg acgacgacga tgtcgtgtcc ttcggcgcgg gctttggcga
     1261 cacgtttggc cacgttttta atgcgttcgg gcgagcctac ggatgtgccg ccgtatttgt
     1321 gtacgattaa cgccatgttt cgtgctttct tgtgtgggat tggtcgggca gcatggtttg
     1381 ctggaaaagc ggctattatt actatttttt acatgaagtt caagaacgga ctgcgctttc
     1441 ccacctgccg tttgacagcg gtcagcaaaa aacctgttct ttcagattgt tgacaaaatg
     1501 ccgtctgaac ggttttcaga cggcatccgg acgacaatca ggcggcagac agcgcatttt
     1561 gctggtgttg cagcagttcg cctatgcctt tttgcgccag tgcaaccagt ttgcccaatt
     1621 cgtccaaact gaacggcgcg tcttccgccg tcccctgtat ttcgatgatt tttcccgatg
     1681 cggtcatcac gatattcaca tcactgtcgc aaccggagtc ttcgggataa tccaaatcca
     1741 aaagcggcac gccgttcact acgcctactg acacagcggc aacggcttcg cggatggggt
     1801 tttcactcaa aatgccgtct gaaaccagtt tgccgacggc gatttgcagc gcgacaaacg
     1861 caccggtaat cgaagccgta cgcgtaccgc cgtctgcctg aatcacgtcg cagtcaatca
     1921 agatttgtcg ttcgccgagt ttttccatat ccacgaccgc gcgcagcgaa cgcccgataa
     1981 ggcgttggat ttcttgtgtg cgcccggact gtttgcccgc cgaagcttcg cggcgcatcc
     2041 gggaagcagt tgaggcaggc agcatcccgt attccgccgt tacccagcct tggtttttac
     2101 cgcgcagaaa cggcgggacg ttttcatcta tggaagcggt acaaatcact ttggtattgc
     2161 cgcattcaat aaggcacgaa ccgtccgtat gcggcaggaa atgaggggtg attttgatat
     2221 cgcgcaggct gtccgcggcg cgcgagatgc ggatgtaatc aggcatactg ccctcccgtt
     2281 aaaaacagat aaattaaaaa gccttaaata tgaaaaatca catttaaggc cttcaaactg
     2341 aaaatttcta cgcctcttcg gctttgctgc ggataatcaa aagcggcagg tggctttggc
     2401 gcattaccgt ttcggcaaaa ctacccatta aaaggtgcat cagtccggta cgtccgtgcg
     2461 tacccaacac cagcaggtcg gcaccgtttt catcggcata atcaaccaaa tcctgcgcca
     2521 tttcacgcgc acccttattg gcaaccagca ggtgtttgac gatattttcc acacccagtt
     2581 cccgggcggt gcgctcggcg gcatccaaaa cttcgttgcc ttgcgcgacg gcggcggctt
     2641 cgtagctttc gtgttgcaaa aattcggggg cgagcgccat atattcggca ggattggcaa
     2701 cgtgtaccaa agtcagacgc gcaccgttga ccccggcaag ctcggcggca tgtttcaggg
     2761 cattgatgga cgtttcactg ccgtcaacgg caacaaccaa atgtttgtac atatcgtatt
     2821 ctccttttgc accgcctcgc ggtgccctct tgtcggatgg gcgcagggac agtttgcgct
     2881 gtttcattat agacccgccg tcgggcttta tacaacagcc gaacagcccg accgctttcc
     2941 agtataatat gccgcttccg tgcagtcagg cattttttgc cggctttcgt tcactttttg
     3001 atttgacgca atcttgcagg attcgaccat gtccgacaac gctttgacct cttcgcgacg
     3061 cttcggcggc atcgccagac tctacggaga ctctgccttg gcgcactttt cacaggcaca
     3121 cgtctgcgta gtcggcgtgg gcggtgtcgg ctcgtgggcg gtcgaggctt tggcgcggac
     3181 gggcatcgga cgtttgactt tgattgattt ggacaacgtt gccgaatcga atgtcaaccg
     3241 ccagctgcac gccctgaccg gagacttcgg caaagcaaaa gttaccgcct tgcgcgaacg
     3301 cgtcgcccaa atcaacccag aatgccaagt gtttgaaatt gaagatttcg ttaccgaaga
     3361 caatttgccg gaatacttcg gaaaaggttt tgatttcgtc atcgacgcga tcgaccaagt
     3421 gcgcgtcaaa gcagcaatgg cggcttattt tgtggaacgc aaacaaccgt ttgtcctcag
     3481 cggcggcgcg ggcggacaaa aaaatccggc gttaatccaa accgccgatt tgagccgcgt
     3541 aacccacgac ccgctgcttg ccaacctgcg ctacaccttg cggaaacgct acggattcag
     3601 ccgcgatacg aaagcaaata tgcgcgtgcc ttgcgtgtat tcgaccgaaa atatcgtgcc
     3661 gccgcagtct agggaggctt gttcggcaga tgccgctccg caaggcttgt cgtgcgccgg
     3721 ctacggtgca agcatgctcg ttaccgcttc gttcgggcta tattgcgcac aggcggcggt
     3781 ggaacacatc gcagacaaaa aataagcaat gccgtctgaa acaggattca gacggcattt
     3841 gaacaaacgc ggcaatcaag ctgtcggttc agaaaaacca acatcggcac gcctaaaacc
     3901 gtattcacaa cctgctcctt ttcaaaacat ttgcatttaa aagccgttat aatgccgtct
     3961 gaacatttgc ccgaccacat tatacgtgaa tgtcggcaga ttgttttctt ttgtaaactt
     4021 atattaaaat ccacttaccg attcacgcca tgccgcccat ccctgcccca tccgcaccat
     4081 ccgagcacac tgtcgcatgg gtattcggcc aacccgttac cgatttgccg caggatttgt
     4141 ttatcccacc cgacgcgctg aaagtcgtat tgggcagttt tcaaggccct ttggatctac
     4201 tgctgtatct gatccgcaag cagaacatcg atgttctcga tattccgatg gtgaagatta
     4261 ccgagcagta tctgcactac atcgcccaaa tagaagccta tcagtttgat ttggcggcgg
     4321 aatacctgct tatggcggcc atgctgattg aaatcaaatc gcgcctgctg ctgccgcgta
     4381 ccgaaaccgt cgaagacgaa gaagccgacc cgcgtgccga gttggtgcgc cgcctgctgg
     4441 cttacgaaca gatgaagctg gcggcgcagg gtttggacgc gctgccccga gccggacggg
     4501 atttcgcgtg ggcttacctg ccgctggaaa ttgccgtcga agccaagctg cccgaagtct
     4561 atattaccga cttgacgcaa gcgtggctgg gtattttgtc tcgggcaaaa cacacgcgca
     4621 gccacgaagt aatcaaagaa accatctccg tgcgcgcgca aatgacggca atcctgcgcc
     4681 gtttgaacgg acacgggata tgcaggtttc acgacctgtt caatcccgaa cagggcgcgg
     4741 cttacgtggt cgtcaacttc atcgcactgt tggagcttgc caaagaaggt ttggtcggaa
     4801 tcgtacagga agtcggtttc ggagaaatcc gaatcagcct caatcatgag ggggcacatt
     4861 cagacggcat ttccggcaca cggggcgggc gcgatgtgtt ctaatacgcc ccaagccgcc
     4921 accaaaaatc gggagacacg ccatatgacc ggcatcatac attcgctgct tgacaccgac
     4981 ctctacaaat tcactatgct gcaagtggtt ctgcaccagt ttccgcagac gcacagcctt
     5041 tacgaattcc gctgccgcaa cgcctcgacc gtctatccgc ttgccgacat cagggaagac
     5101 ttggaagccg aactcgacgc gctctgccaa ctacgcttca cccacgacga actcgactac
     5161 ctccgtagcc tgcgtttcat taaaagcgac tttgtcgatt atctcgaact cttccagctc
     5221 caacgccgct ttgtcgaaat cggcacagac gataaagacc gtctgaacat ccgcatcgaa
     5281 ggtccgatga tacaggcgat gttttttgaa atcttcatcc tcgccattgt caacgaactt
     5341 tacttccgcc gcctggaaac ccctgcagtc atagaagaag gcgaacgccg gcttcaagcc
     5401 aaagccgcgc gcctcaaaga aatcgccgcc gcacaaaacc ccgacgaacc gcccttcctg
     5461 atttccgact tcggcacgcg ccgccgctac aagctcgcgt ggcaggaaca cgtcatccgc
     5521 accctgctcg aagccgcccc aagcatcgtg cgcggcacca gcaatgtctt tctcgccaaa
     5581 aaactcggca tcacccccat cggcaccatg gcgcacgagt tcctccaagc cttccaggcc
     5641 ctcgacgtac gcctgcggaa tttccaaaag gccgcgctcg aaagctgggt gcacgaatac
     5701 cggggcgatt tgggcgttgc cctgaccgac gtggtcggta tggatgcctt cctgcgcgat
     5761 ttcgacctct atttcgccaa acttttcgac gggctgcgcc acgacagcgg cgacccttac
     5821 gtttggggcg acaaagccta cgcccactat caaaagctca aaatcgacag ccgcaccaaa
     5881 atgctgacct tctccgacgg gctggacatc gaacgctctt gggcattaca ccaatatttc
     5941 aaagaccgct tcaaaaccgg cttcggcatc ggcaccaacc tgaccaacga tatggggcat
     6001 acgcctttga atatcgtctt gaaactggtc gaatgcaacg ggcagtccgt cgccaagctg
     6061 tccgactctc cgggcaaaac catgaccaac aacagcacct tcctcgccta cctgcgccaa
     6121 gtgttcgacg tacccgaacc cgaaacgccg taaaccggca gaaaaagcgc acaattcctg
     6181 tttctgccgc ataaaatctt ttaaaatacc gcctgatttg aatttaaccg aaagaccgaa
     6241 cttcatgaac ctacatcaaa ccgtcgaaca cgaagccgcc gccgcctttg ccgccgcagg
     6301 catcgccggc agccccgttg ttttgcagcc gaccaaaaat gccgaacacg gcgatttcca
     6361 aatcaacggc gtgatgggtg cggcgaaaaa agccaaacaa aacccgcgcg aactggcgca
     6421 aaaggtcgcc gacgcattgg caggcaacgc cgtcatcgaa agcgcggaag tcgccggtcc
     6481 cggttttatc aacctgcgcc tgcgccccga atttctcgcg caaaacattc agacggcctt
     6541 gaacgacgcg cgtttc
//
LOCUS       DAWHSK010000104         6518 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.104, whole genome shotgun sequence.
ACCESSION   DAWHSK010000104 DAWHSK010000000
VERSION     DAWHSK010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6518
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.104"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(41..961)
                     /gene="truB"
                     /locus_tag="WIC03_001265"
     CDS             complement(41..961)
                     /gene="truB"
                     /locus_tag="WIC03_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEZ3883761.1"
                     /translation="MTNKPAKRPVNGVLLLDKPEGLSSNTALQKARRLFHAEKAGHTG
                     VLDPLATGLLPVCFGEATKFAQYLLDADKAYTATLKLGEASSTGDAEGEIVATARADI
                     SLAEFQTACKALTGDIRQVPPMFSALKHEGKPLYEYARKGIVIERKARDITVYAIDIA
                     EFDAPKAVIDVRCSKGTYIRTLSEDIAKHIGTFAHLTALRRTETAGFTIAQSHTLEAL
                     ANLNETERDSLLLPCDVLVSHFPQTVLNDYAVHMLHCGQRPRFEEDLPSDTPVRVYTE
                     NGRFVGLAEYQKEICRLKALRLMNTAASAA"
     gene            complement(1019..1543)
                     /locus_tag="WIC03_001266"
     CDS             complement(1019..1543)
                     /locus_tag="WIC03_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883762.1"
                     /translation="MNNLIPEHLAAYAHSDSLQIEGRHRCFSLSCQGRDTFHIRYYGE
                     PFDGLITDTDKAPVKIVAVEAVSGDEIVLFDGAEHGYNAMFCDKYSPNQKQNRMLTDL
                     DEYTYRVLIHLYYNIDYEDEYEDFVNSEGQVPLIDGRIISFDSLKRNGFDAISVDLID
                     EKHSVRELLNEELS"
     gene            complement(1554..1925)
                     /gene="rbfA"
                     /locus_tag="WIC03_001267"
     CDS             complement(1554..1925)
                     /gene="rbfA"
                     /locus_tag="WIC03_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEZ3883763.1"
                     /translation="MRKPQRGYARQDRVKEQIMRELAELVRTGLKDPRAGFITVNEVE
                     VTRDYSHATVFYTVLNQDAREITEEVLEHARGHLRSELSKRIKLFKTPELHFKYDESL
                     ERGMNLSALIDQVAAEKPVED"
     gene            2115..3359
                     /gene="clpX"
                     /locus_tag="WIC03_001268"
     CDS             2115..3359
                     /gene="clpX"
                     /locus_tag="WIC03_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEZ3883764.1"
                     /translation="MSNENRTCSFCGKSKSHVKHLIEGENAFICDECVSNCIEILHED
                     GNDGTPSESAGGEPEESGKLPTPAEIVANLNDHVIGQEQAKKALAVSVYNHYKRLRHP
                     KAGANVELSKSNILLIGPTGSGKTLLAQSLARKLDVPFVMADATTLTEAGYVGEDVEQ
                     IITKLLGKCDFDVEKAQRGIVYIDEIDKISRKGDNPSITRDVSGEGVQQALLKLIEGT
                     VASVPPQGGRKHPNQEFINVDTTNILFICGGAFAGLEKVIRQRTEKGGIGFGASVHSK
                     DENADITKLFGIVEPEDLIKFGLIPELIGRLPVIATLEELDEDALINILTEPKNALVK
                     QYQALFGMENVELEFEEGALRSIARQAMERKTGARGLRSIVERCLLDTMYRLPDLKGL
                     KKVVVGKAVIEEGREPELVFES"
     gene            complement(3462..4658)
                     /locus_tag="WIC03_001269"
     CDS             complement(3462..4658)
                     /locus_tag="WIC03_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEZ3883765.1"
                     /translation="MQNYLTPNFAFAPMIPERASGSRVWDTEGREYIDFSGGIAVNAL
                     GHCHPALADALNAQMHKLWHISNIYTTRPAQELAQKLVANSFADKVFFCNSGAEANEA
                     ALKLARKYARDRFGGGKSEIVACINSFHGRTLFTVSVGGQPKYSKDYAPLPQGITHVP
                     FNDIAALEAAVGEQTCAVIIEPIQGESGILPATAEYLQTARRLCDRHNALLILDEVQT
                     GMGHTGRLFAYEHYGVVPDILSSAKALGCGFPIGAMLATEKIAAAFQPGTHGSTFGGN
                     PMACAVGSRAFDIINTPETLNHVREQGQKLQTALLDLGRKTGLFSQVRGMGLLLGCVL
                     DEAYRERASEITAAALKHGVMILVAGADVLRFAPSLLLNDEDMAEGLRRLEHALTEFA
                     ATSDNP"
     gene            5062..5562
                     /locus_tag="WIC03_001270"
     CDS             5062..5562
                     /locus_tag="WIC03_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883766.1"
                     /translation="MNAMPQTAAQNETLHICSIIVDDAQDFSPMLLDAGRDDTLISES
                     MIPQIRTVAALIAAERHDFSRSSPAEFTDAADFFAARILVLGVRRFHLDVSLLQILKT
                     ANSRARRFAEKHRLFFTPAQAELSLNKHKNRRLLTVETEHEVENKGNPVANSLAFVRK
                     LHTLPL"
     gene            5647..6201
                     /locus_tag="WIC03_001271"
     CDS             5647..6201
                     /locus_tag="WIC03_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883767.1"
                     /translation="MKKIIASALIATFALAACQDDTQARLEQQQKQIEALQQQLAQQA
                     DDTVYQLTPEAVKDTIPAEAQANGNNGQPVTGKDGQQYIYDQSTGSWLLQSLVGAAAG
                     AFIGNALANKFTRAGNQDSPVARRARAAYHQSARPNARTSRDLNTRSLRAKQQAAQAQ
                     RYRPTTRPPANYRRPAMRGFGRRR"
BASE COUNT         1599 a         1714 c         1694 g         1511 t
ORIGIN      
        1 aacacattat tcaaacacag cctgtatttt taaccgccgt tcaggcggcg gatgccgccg
       61 tgttcatcag gcgcaaggct ttcagacggc atatttcttt ttgatattcc gccagaccga
      121 caaagcggcc gttttccgtg taaacgcgta ccggcgtgtc ggaaggcagg tcttcttcga
      181 aacgcggacg ttgtccgcag tggagcatat ggacggcata atcgtttaaa acggtttggg
      241 gaaagtgtga aaccaatacg tcgcagggta gcagcaagct gtcgcgttct gtttcgttca
      301 aatttgccaa ggcctcaagc gtgtggcttt gggcgatggt aaagccggcg gtttcagtgc
      361 ggcgcagggc ggtcaggtgg gcgaacgtgc cgatgtgttt ggcgatgtct tcgctgaggg
      421 tgcggatgta ggtgcctttg ctgcaacgta cgtcgatgac ggctttgggc gcgtcaaatt
      481 cggcaatatc gatggcgtaa acggtaatgt cgcgcgcttt gcgttcgatg acgatgcctt
      541 tgcgcgcgta ttcgtacagc ggtttgcctt cgtgcttgag cgcggaaaac attggcggca
      601 cttggcggat gtcgcccgtt aatgctttgc aggccgtctg aaattcggct aaggaaatat
      661 cggcacgggc ggtggcaacg atttcgcctt cggcatcacc cgtgctgctg gcttcgccga
      721 gtttcagcgt ggcggtgtag gctttgtcgg catccagcag gtattgggcg aacttggtcg
      781 cttcaccgaa gcagacgggc aaaagtccgg ttgccaaagg gtcgagcacg ccggtatgtc
      841 cggctttttc ggcatgaaac aaacgccgcg ctttttgcag cgcggtgttg ctggaaaggc
      901 cttcgggttt gtcgagaaga agaacgccgt tgaccgggcg tttggcgggt ttattggtca
      961 tattaattag ggaatattaa tgttcagacc gtctgaaagg gttcagacgg cctgcgtgtt
     1021 agcttaattc ttcattcaat aattcacgaa cagagtgttt ttcatcaatt agatcaacgc
     1081 tgattgcatc aaagccattt cgttttaatg agtcaaaact aatgattcgg ccatcaatta
     1141 aggggacttg tccttcagaa ttgacgaaat cttcatattc atcttcatag tctatattgt
     1201 aataaagatg aatcagaact cggtaagtat actcatccaa atcagttaac attctgtttt
     1261 gcttttgatt tggactatat ttgtcgcaaa acatagcgtt atagccatgt tccgcgccat
     1321 caaacaatac gatttcatcg ccgcttacag cttctaccgc cacaattttt accggagcct
     1381 tatcagtatc ggtaatcaat ccatcaaaag gctctccata gtaacggatg tggaaagtat
     1441 ctctaccttg gcaggataat gaaaagcaac gatgcctgcc ttcaatttgc aggctatcac
     1501 tatgtgcata ggcggctaaa tgttcgggaa tcagattgtt cattccagca gtatcagtct
     1561 tcaaccggtt tttccgccgc cacttgatcg ataagggcag acaggttcat accacgctct
     1621 aaggattcgt cgtatttgaa atgcagttcg ggcgttttga acagtttgat gcgtttggac
     1681 aattcgctgc ggaggtgtcc gcgcgcgtgt tccagcactt cttccgtaat ttcgcgcgcg
     1741 tcttggttta acaccgtata gaacacggtg gcatggctgt aatctcgggt aacttcgact
     1801 tcgttgacgg taatgaagcc ggcgcgcggg tctttcagtc cggtacggac gagttcggca
     1861 agctcgcgca taatttgttc tttgacacgg tcttggcggg catagccgcg ttggggtttt
     1921 ctcatgaata tcctcagatt tcagatttgg tttcagacgg catttttgcc gccggggcgg
     1981 ttgcgaaagg gcgtattata gcctaatcgg cgggatttta aggtaataca ttgataagaa
     2041 cggatatagt tattatactt ggcaacccga acggcacgtg ccgtttctgt aacgcataca
     2101 ttgtaaagaa agccatgtcc aacgaaaacc gtacctgttc cttttgcgga aaatccaaat
     2161 cacacgtcaa acatttaatt gagggcgaaa acgcctttat ctgtgacgaa tgcgtctcaa
     2221 actgtattga aatattgcat gaagacggaa atgacggcac gccttcggaa agtgccggag
     2281 gggagccgga agaatccggc aagctgccca cgcccgccga aatcgtcgcc aacctcaacg
     2341 accacgttat cggtcaggaa caagccaaga aagccttggc ggtgtcggtg tacaaccatt
     2401 acaaacgcct gcgccacccg aaagccggag ctaatgtcga attgtcgaaa tccaacatcc
     2461 tgcttatcgg cccgaccgga tcgggtaaaa ccctgctggc gcaatctttg gcgcgtaaac
     2521 tggacgtacc gttcgtgatg gcggatgcaa ccacgctgac tgaagcaggt tatgtcggag
     2581 aagatgtcga gcaaatcatc accaaactgt tgggcaaatg tgatttcgat gttgaaaaag
     2641 cccagcgcgg catcgtctat atcgacgaaa tcgataaaat ttcacgcaaa ggcgacaatc
     2701 cgtccatcac gcgcgacgta tccggcgaag gcgtgcaaca agccttgcta aaactgattg
     2761 aaggcacggt agccagcgtt ccccctcaag gcggacgcaa acatccgaac caagaattca
     2821 tcaacgttga taccaccaac atcctcttta tctgcggcgg cgcatttgcc ggcttggaaa
     2881 aagtcatccg ccaacgtact gaaaaaggcg gtatcggctt tggtgcgtcc gttcacagca
     2941 aggacgaaaa tgccgacatt accaagctgt tcggcatcgt cgaaccggaa gacttgatta
     3001 aattcggtct gattccagaa ttaatcggac gtttgcctgt gattgcaact ttagaagaat
     3061 tggacgaaga cgcgctgatt aatattttga ccgagccgaa aaatgctttg gtcaagcagt
     3121 atcaggcgtt gttcggtatg gaaaatgtcg aactggagtt tgaagaaggc gcattgcgtt
     3181 ccatcgcgcg tcaggcaatg gaacgcaaaa ccggcgcgcg cggcctgagg tccatcgtcg
     3241 aacgctgcct gctcgatacg atgtaccgcc tgcctgattt gaaaggcttg aaaaaagtgg
     3301 tggtcggtaa ggcagtcatc gaagagggca gggaaccgga attggtgttc gagtcctgat
     3361 ggcaaacaaa accgcttcgg aacgttgaac cacgtttctg aagcggtttt ttgtttctga
     3421 tgccgtctga agccttcccg ccttcagacg gcatttgagt tttacggatt gtctgatgtc
     3481 gcggcaaatt ccgtcagcgc gtgttccaaa cgtcgcaaac cttccgccat atcctcatcg
     3541 ttcaacagta gcgaaggcgc gaaacgcaat acgtccgcac ccgcaaccaa aatcatcacg
     3601 ccgtgtttca aggcggcggc ggtgatttcg gatgcgcgtt cgcgataggc ttcgtccaac
     3661 acgcagccga gtagcagccc catcccgcga acctgtgaga acaagcccgt tttcctgccc
     3721 aaatccagca atgccgtctg aagtttctgc ccctgttcac ggacatggtt taaagtttcg
     3781 ggcgtattga tgatgtcgaa tgcgcggctg ccgaccgcac acgccatcgg gttgccgccg
     3841 aaagtcgagc cgtgcgtgcc cggttggaag gcggcggcaa tcttttctgt cgccagcatc
     3901 gcgccgatcg gaaagccgca gcccaaggct tttgccgaac tcaaaatatc gggaacaacg
     3961 ccgtaatgtt cataggcaaa cagcctgccc gtatgcccca tcccggtttg aacttcgtcc
     4021 aaaatcaaca acgcattgtg ccggtcgcac agacggcgcg cggtttgcaa atattccgca
     4081 gtggcgggca ggatgccgct ttcgccctgt atcggctcga tgatgaccgc gcaggtctgt
     4141 tcgccgacgg cagcttccag cgcggcaata tcgttgaacg gaacgtgcgt aatgccttgc
     4201 ggcagcggtg cataatcctt gctgtatttc ggctgaccgc cgacggacac ggtaaacagc
     4261 gtgcgtccgt ggaaactgtt gatacaggcg acgatttcgc tttttcctcc gccgaaacgg
     4321 tcgcgcgcat atttccttgc cagcttcaac gccgcctcat tcgcttccgc gcccgagttg
     4381 cagaaaaaaa ccttgtcggc aaaactgttt gcaaccaatt tttgcgccaa ttcctgcgcc
     4441 ggacgcgtcg tatagatatt ggaaatgtgc cacagcttgt gcatctgcgc gtttaaagca
     4501 tcggcaaggg cagggtggca gtgtcccagc gcattgacgg cgataccgcc tgaaaaatca
     4561 atatattcac gcccttccgt atcccaaacg cggctgcctg aagcgcgttc gggaatcatc
     4621 ggggcaaagg cgaaattggg ggtcagatag ttttgcataa ttgctcctga ttgcggtttt
     4681 gctcgggatt atgcaccgtt tttcaaagca gataaaggga aattgccgac aatatcgggc
     4741 agcggtaaaa ataaaccgaa atattttcaa tcggatagat gaaaaatcaa aaaaatttcg
     4801 ataaaatgct tgaggcgcgg cagcgttttg atttagaatc tccccaatat ttttatcttc
     4861 agacggcatt tcatcatgca aggcaaaaaa tttacaaccg cttattgcta ttggtaccac
     4921 acagacgtgc ggtacttttt tgcgcttgct tgaaatccat cccatacgca aaacaggccg
     4981 ctcgaaaacg gggcggcttt tttgttacct tcacacaggt cgcccgattc ggcaacacac
     5041 gacagaatca ggaaaatgac catgaatgca atgccccaaa ccgccgcaca aaacgaaacc
     5101 ctgcacattt gcagcattat tgtcgatgat gcacaggatt tttcccccat gcttctcgat
     5161 gcagggcggg acgacacact tatcagcgaa tcgatgattc cgcaaatccg cactgttgcc
     5221 gccctgatag ccgccgaacg gcacgacttc agccgcagca gccccgccga gtttaccgat
     5281 gccgccgatt tttttgccgc acggattctc gtattgggcg tgcgacgctt ccatctggac
     5341 gtatcgctgc ttcagatttt gaaaaccgcc aacagccgcg cccgccgctt tgccgaaaaa
     5401 caccgcctgt ttttcacacc cgcacaagcc gaattgagcc tgaacaaaca caaaaaccgc
     5461 cgcctgctga ccgtagaaac cgaacacgaa gtcgaaaaca aaggcaaccc agtcgccaac
     5521 agcctcgcat ttgtccgcaa actgcacacc ctgcccctgt aaacatcacg atttgccggc
     5581 agaatgcttg ccaaaattcc cgacggcgat acaataaaac cgattttcca cccaaacagg
     5641 acacacatga aaaaaatcat cgcctccgcg cttatcgcaa cattcgcact cgccgcctgc
     5701 caagacgaca cgcaggcgcg gctcgaacag cagcagaaac agattgaagc cctgcaacag
     5761 cagctcgcac agcaggcaga cgatacggtt taccaactga ctcccgaagc agtcaaagac
     5821 accattcctg ccgaagcaca ggcaaacggc aacaacgggc aacccgttac cggtaaagac
     5881 gggcagcagt atatttacga ccaatcgaca ggaagctggc tgctgcaaag cctggtcggc
     5941 gcggcggcag gcgcgtttat cggcaacgcg ctggcaaaca aattcacacg ggcaggcaac
     6001 caagacagtc ccgtcgcccg gcgcgcgcgt gcagcctacc atcagtccgc acgccccaat
     6061 gcgcgcacca gcagggattt gaacacgcgc agcctccgcg caaaacaaca ggcggcgcag
     6121 gcgcagcgtt accgcccgac aacgcgcccg cccgccaatt accgccgacc ggctatgcgc
     6181 ggtttcggca ggaggcggta aacccggcgc gccaatgccg tctgaagggc tttcagacgg
     6241 cattttgttt ccaagagttt gatgtcggga tggcaattct gatttacgtg cgttggcagg
     6301 gcggcattgt catgccgccg ttgggttttc agacggcatc atatcgaacc gtcaggtcgt
     6361 ggggtgagca ttcttgccca ccgttgtaag ggcgggattt tttggtgaac aggaatgccc
     6421 accctgcatc tgaaaccctt ctgcgtcatt cccacgcagg aaggaattta gatatttgaa
     6481 tttacagaaa ttttcaatcg ttgccaaagc ggaaaagg
//
LOCUS       DAWHSK010000105         6460 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.105, whole genome shotgun sequence.
ACCESSION   DAWHSK010000105 DAWHSK010000000
VERSION     DAWHSK010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6460
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.105"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(265..1650)
                     /gene="pntB"
                     /locus_tag="WIC03_001272"
     CDS             complement(265..1650)
                     /gene="pntB"
                     /locus_tag="WIC03_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HEZ3883768.1"
                     /translation="MSSGLVTAAYIVAAILFIFSLAGLSKQETAKQGCYSGIAGMAVA
                     LFVTVFSDNTHGLGWIIIAMLIGAAIGIYKAKKVEMTEMPELIALLHSFVGLAAVLVG
                     FNSYIAPGNVSHDMHTIHLVEVYLGIFIGAVTFTGSLVAFGKLNGKISSSPLQLPAKH
                     KLNALALAVSFVLLLVFVGIDGSGFILLIMTLIALAFGWHLVASIGGADMPVVVSMLN
                     SYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFVSVIAGGFGSDSG
                     TLSSGSQEIGEYREVKAADIAEMLKGANNVIITPGYGMAVAQAQYPVAEITELLRKNG
                     TEVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFPETDVVLVIGANDTVN
                     PAAQTDPNSPIAGMPVLEVWKAKEVVVFKRSMNTGYAGVQNPLFFNENSVMCFGDAKK
                     TVDDILSELKK"
     gene            complement(1730..2113)
                     /locus_tag="WIC03_001273"
     CDS             complement(1730..2113)
                     /locus_tag="WIC03_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEZ3883769.1"
                     /translation="MTFAYWCILIAYLLPLFCAAYAKKAGGFRFKDNHNPRDFLARTQ
                     GTAARAHAAQQNGFEAFAPFAAAVLTAHATGNAGQATVNTLAGLFILFRLAFIWCYIA
                     DKAALRSLMWAGGFVCTVGLFVAAA"
     gene            complement(2117..3658)
                     /locus_tag="WIC03_001274"
     CDS             complement(2117..3658)
                     /locus_tag="WIC03_001274"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEZ3883770.1"
                     /translation="MKIGIPRESLSGETRVACTPATVALLGKLGFETVVESGAGLAAS
                     LDDAAYQTAGATVADKAAVWACPLIYKVNAPSEQELPLLNEGQTIVSFLWPRQNEALV
                     EALRAKKVNALAMDMVPRISRAQALDALSSMANISGYRAVIEAANAFGRFFTGQITAA
                     GKVPPAQVLVIGAGVAGLAAIGTANSLGAVVRAFDTRLEVAEQIESMGGKFLKLDFPQ
                     ESGGSGDGYAKVMSDEFIAAEMKLFAEQAKEVDIIITTAAIPGKPAPKLITKEMVESM
                     KSGSVIVDLAAATGGNCELTKPGELFVTDNGVKIIGYTDMANRLAGQSSQLYATNLVN
                     LTKLLSPNKDGEITLDFEDVIIRNMTVTRDGEITFPPPPIQVSAQPQQTSSEKAAPAA
                     KPEPKPVPLWKKLAPAVIAAVLVLWVGAVAPAAFLNHFIVFVLACVIGYYVVWNVSHS
                     LHTPLMSVTNAISGIIVVGALLQISQGNGFVTLLAFIAILIASINIFGGFAVTRRMLN
                     MFKKG"
     gene            3948..4781
                     /gene="serB"
                     /locus_tag="WIC03_001275"
     CDS             3948..4781
                     /gene="serB"
                     /locus_tag="WIC03_001275"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEZ3883771.1"
                     /translation="MPHALVLQFPSAEVLPSDFPSRLPEPDCADEKRMRFIVEEGFSL
                     SEKDAALLGSRQIDHAVLPDMDFGELGLIVSDMDSTLITIECVDEIAAGVGLKDKVAE
                     ITERSMRGELDFEQSLRSRVALLAGLDERVLADVYENILKLSPGAEFLLDECKRHDVK
                     FLLVSGGFTFFTERLQQRLGFEYQHANVLEIENGRLTGRLKGRIIDAQAKADLLLEYR
                     SRLGLQPHQVLAMGDGANDILMLKETGIGVAYRAKPKVRAAADVCINFGGLERVRGLF
                     R"
     gene            4847..6070
                     /locus_tag="WIC03_001276"
     CDS             4847..6070
                     /locus_tag="WIC03_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEZ3883772.1"
                     /translation="MGRRKLWFALAAAGVIGGLVGIVLTELMHFIQHTAYGYGADGAY
                     ISFREGVARASEGRRLGVLILCGMLAGGGWWLLKRFGKPQIEIKAALKQPLQGLPFLT
                     TVFHVLLQIITVGLGSPLGREVAPREMTAAFAFAGGRRLGLDEDEMRLLIACASGAGL
                     AAVYNVPLASTLFILEAMLGVWTQQAVAAALLTSVIATAVARIGLGDVQQYHPANLTV
                     STSLLWFSAVIGPILGATAVFFQRTAQKFPFIKRDNIRIILLAVCMFALIGVISVWFP
                     EVLGNGKAGNQLTFSGLTGWQYALELAAVKWPVVLMALAAGAYGGLITPSMMLGSTVS
                     FAAAAAWNSVFPAMPSESAAVVGAAVFLGVSLKMPLTAIVFVLELTYAPLSLLLPLCI
                     GMAGALGTSGKMGFK"
     gene            6230..>6460
                     /gene="purH"
                     /locus_tag="WIC03_001277"
     CDS             6230..>6460
                     /gene="purH"
                     /locus_tag="WIC03_001277"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360677.1"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEZ3883773.1"
                     /translation="MSVIKRALISLSDKAGAVEFAQTLHKLGVEILSTGGTAKLLADA
                     GVPVIEVADYTGFPEMLDGRVKTLHPKIHGGIL"
BASE COUNT         1390 a         1691 c         1861 g         1518 t
ORIGIN      
        1 caagtggaaa aatacttggc agattatcag gcacacttgg aaaaagtgtt cggctaatta
       61 aaaatccatc ttcaacacgg agatggattt tgtttgtttc gttgattttg tgtcagtttc
      121 agatgtaggt gcttattcgg acgggccgtc tgaaaatgtt tgccccaatg caaaaaaatc
      181 actgcaaacc ttcataaacg gggtttgcag tgattttttc aaatcaaaca gattgaaaac
      241 ctgcgccgaa ttgttcagac ggcattattt tttcagttcg gacagaatgt catctacggt
      301 tttcttcgca tctccgaaac acatcacgct gttttcgttg aagaacagtg ggttttgtac
      361 acctgcgtag ccggtattca tcgagcgttt gaagacgacg acttcttttg ccttccacac
      421 ttccaacacg ggcatacccg caatcgggct gttcgggtcg gtttgggcgg cggggttgac
      481 ggtgtcgttc gcaccgatga ccaagaccac atcggtttcg gggaagtcgt cgttgatttc
      541 gtccatttcc aaaacgatgt cgtaggggac tttggcttcg gcgagcagta cgttcatatg
      601 accgggcagg cggccggcga cggggtggat gccgaagcgt acttcggtgc cgtttttacg
      661 taaaagctcg gtgatttcgg caacggggta ttgcgcttgt gcgactgcca taccgtagcc
      721 cggggtaatg atgacattgt ttgcgccttt cagcatttcg gcaatatcgg cagctttgac
      781 ttctcggtat tcccctatct cttggctgcc ggaagataat gtgccgctgt cgctgccgaa
      841 accaccggca attaccgaga caaacgagcg gttcatggct ttgcacataa tgtaggacag
      901 aatcgcgccg cttgagccga ccagcgcgcc ggtaacgatg agcaggtcgt tggagagcat
      961 gaagcctgcc gctgcggccg cccagccgga gtaggagttg agcatggaca cgaccacggg
     1021 catatctgcg ccgccgatgg aggcaaccaa gtgccagccg aatgcgaggg caatcagggt
     1081 cataatcagc aggatgaagc cgctgccgtc aatgccgaca aatacgagca gcaacacaaa
     1141 cgatacggca agtgccagtg cgttgagctt gtgtttggcg ggcagttgca gcgggctgct
     1201 gctgattttg ccgttgagtt tgccgaatgc gaccagcgag ccggtaaagg ttaccgcgcc
     1261 gatgaagatg cctaaataca cttcgaccag atggatggtg tgcatatcgt gcgaaacgtt
     1321 gcccggcgcg atatagctgt tgaagccgac caaaaccgcc gctaggccga cgaagctgtg
     1381 cagcagggca atcagttcgg gcatttcggt catttccacc tttttggctt tgtagatgcc
     1441 gattgccgcg ccgatgagca tggcgatgat gatccagccc agtccgtggg tattgtcgga
     1501 aaaaacagtt acaaaaaggg cgaccgccat accggcgata ccggaatagc agccctgttt
     1561 ggcggtttcc tgcttggaca gccccgccag cgaaaagata aataaaattg cggcaacgat
     1621 atacgccgct gttacgagtc ctgaagacat ggaaattctc cgattttcga tgatttgttt
     1681 tcaatgccgt ctgaaaaatt gacgttcgtg ttttcagacg gcatctgttt caagcagccg
     1741 cgacaaacag cccgacggtg cagacaaatc cgcccgccca catcagcgag cgcaatgctg
     1801 ctttgtcggc gatgtagcac cagataaagg cgaggcggaa caggatgaac aggccggcaa
     1861 gcgtgttgac ggttgcttgt ccggcattgc cggttgcatg tgccgtcaaa acggcggcag
     1921 caaacggtgc aaaggcttca aaaccgtttt gctgcgcggc gtgggcacgg gcggctgtgc
     1981 cttgcgtgcg cgccagaaaa tcgcgcggat tgtggttgtc tttaaaccgg aatccgcccg
     2041 cttttttggc atacgccgca caaaaaagcg gcaataggta ggcaatcaga atacaccaat
     2101 aggcgaaagt catggcttac cctttcttaa acatattcag catacgccgt gttaccgcaa
     2161 agccgccgaa gatgttgatg ctggcaatca ggatggcgat gaaggcaagc agggtaacga
     2221 agccgttgcc ttggctgatt tgcagcagcg cgccgacgac gatgatgccg gagatggcgt
     2281 tggtcacgga catcagcggg gtatgcagcg agtggctgac gttccagacg acgtagtagc
     2341 cgatgacgca ggcgaggacg aacacgatga agtggttcaa aaatgctgcg ggcgcgaccg
     2401 cgccgaccca cagcaccaac acggcggcga tgacggcggg cgcgagtttc ttccacaggg
     2461 gaacgggttt cggctccggc ttggcggcag gcgcggcttt ttcagacgac gtttgctgcg
     2521 gctgggcgga aacttgaatc ggcggaggcg ggaaggtgat ttcgccgtcg cgggtaacgg
     2581 tcatgttgcg gataatcacg tcttcgaagt ccagcgtgat ttcgccgtct ttgttcgggc
     2641 ttaacagctt ggtcaggttc accaagttgg tggcgtaaag ctgggaagac tgtccggcaa
     2701 ggcggtttgc catgtcggtg tagccgatga ttttcacgcc gttgtcggtt acgaacaatt
     2761 cgcccggttt ggtgagttcg cagttgccgc ccgtcgccgc cgccaaatcg acgatgacgg
     2821 agccggattt catgctttcc accatttctt tggtaatcag cttgggcgcg ggtttgcccg
     2881 gaatggcggc ggtagtgatg atgatgtcca cttcttttgc ctgctcggca aagagcttca
     2941 tctcggcggc gataaattcg tcgctcatca ctttggcgta gccgtcgccg ctgccgcccg
     3001 attcttgcgg gaagtcgagt ttcaggaact tgccgcccat cgattcgatt tgttccgcca
     3061 cttccaagcg ggtatcgaac gcgcgtacca ctgcgccgag cgagtttgcc gtaccgatcg
     3121 ccgccaaacc cgccacacct gcaccaatca ccaaaacctg cgcgggcggc actttgccgg
     3181 cggcggtaat ttgaccggtg aagaaacggc cgaaggcgtt ggcggcttca atcacggcgc
     3241 ggtagccgct gatgtttgcc attgaagaca aagcgtccaa agcctgcgcg cgcgaaatgc
     3301 ggggcaccat atccatcgcc agcgcgttca ctttcttggc gcgcaaggct tcgaccaaag
     3361 cctcgttttg gcgcggccac aggaagctga cgatggtttg accttcgttc aaaagcggca
     3421 gttcctgttc ggacggcgcg ttgaccttat aaatcaaagg gcaggcccaa accgccgctt
     3481 tgtcggcaac ggttgcgcct gctgtttggt aagcggcatc gtccaaactt gccgccaaac
     3541 ctgcaccgct ttcgacaacg gtttcaaagc ccagtttgcc caacagggca acggtggcgg
     3601 gcgtgcaggc gacacgggtt tcgccggata atgactcgcg tgggataccg attttcatct
     3661 ctgaatcctt tttcgggttg tttatatgta tcgtgggtta aatttaaatc ggggcagggc
     3721 ggagcaacgc cgtaccggtt taaagccgac tcacttcaaa tgttaatata ttttagataa
     3781 tccccttata acgaattttc atcaggctgg caatagttgc ggcattttcc cgtgttgtcc
     3841 gacacatact gccgtttgcg tttgaagtca ttttccgatt gcagtcccgc ccgccgtaac
     3901 attctttgcc gtttcactat ataatccgca ttttttgagc cgccgttatg ccgcacgccc
     3961 tcgtcctcca atttccctcc gccgaagtcc tgccttccga cttcccctca cgcctgcccg
     4021 aacctgattg cgccgatgaa aagcgtatgc gttttatcgt tgaagaaggg ttttctttaa
     4081 gcgaaaaaga cgcggcgttg cttggcagcc gtcaaatcga ccacgccgtg ttgccggata
     4141 tggatttcgg cgaactcggt ttgattgtca gcgatatgga ttcgacgctg attaccatcg
     4201 aatgcgtcga tgaaattgcg gcaggcgtgg gtttaaaaga caaagtagcg gaaattaccg
     4261 agcgttcgat gcgcggcgaa ctcgatttcg aacagtcttt acgcagccgc gtcgcgctgt
     4321 tggcgggatt ggacgaacgg gttttggcgg atgtttatga aaacattttg aagctctcgc
     4381 ccggtgcgga atttttgttg gacgaatgca aaaggcacga tgtgaaattc ctgctggtgt
     4441 cgggcggctt cacgtttttt accgaaaggc tgcaacaacg cctcggcttc gaataccagc
     4501 acgccaatgt tttggaaatt gaaaacggca ggctgaccgg ccgtctgaaa ggtagaatca
     4561 tcgacgcgca ggcaaaggca gatttattgc tcgaataccg cagccgcctc ggattgcagc
     4621 cacatcaggt gttggcgatg ggcgacggtg cgaacgatat tctgatgctc aaagaaacgg
     4681 gcataggcgt ggcttaccgt gccaaaccga aagtgcgggc agccgccgat gtctgtatca
     4741 acttcggcgg tttggagcgt gtacgcggcc tgttcaggta ggcggatagg aaacggatgt
     4801 cgtctgaaaa acgtgttcag acggcatttg aacggcagga acgacagtgg gacgcagaaa
     4861 actttggttt gccctggcag cagcaggcgt tatcggcggt ttggtcggca ttgtgctgac
     4921 ggaactgatg cacttcatac agcatacggc atacggttat ggcgcggacg gtgcgtacat
     4981 ctcgttccgc gaaggtgtgg cccgtgcttc ggaagggcgg cgtttaggcg tattgattct
     5041 gtgcggtatg ttggcaggcg gcggctggtg gttgctcaaa cgtttcggta agccgcaaat
     5101 cgaaatcaaa gccgccttga agcagccgtt gcaggggctg ccgtttctga cgacggtttt
     5161 ccatgttctg ctgcaaatca taacggtcgg actcggttca ccgctcggac gggaagtcgc
     5221 cccgcgtgaa atgactgccg cgttcgcttt tgccggcggc aggcggttgg gtttggatga
     5281 agacgaaatg cggctgctga ttgcttgcgc ttcgggtgcg ggtttggcgg ccgtgtataa
     5341 cgtgccgctc gcctccacac tttttatcct cgaagccatg ctgggcgtgt ggacgcagca
     5401 agccgtcgcc gccgcattgc tgacttccgt catcgccacc gccgtcgcgc gcatcggctt
     5461 aggcgacgtg cagcaatatc atccggctaa ccttaccgtc agcacctcat tactttggtt
     5521 ttccgccgtc atcggcccga tactgggcgc aaccgccgtc tttttccagc gcacagccca
     5581 aaagtttccc tttatcaagc gcgacaatat tagaatcatt ctcttggcag tctgcatgtt
     5641 tgcgctcatc ggcgtgattt ccgtttggtt tcccgaagtt ttgggcaacg gcaaagcagg
     5701 caaccaactg acttttagtg gtttgaccgg ttggcaatac gctttggaac ttgccgccgt
     5761 caaatggccg gtcgtcttga tggcgctggc ggcaggcgca tacggcggac tgattacgcc
     5821 gtcgatgatg cttggcagca ccgtttcttt tgctgccgcc gccgcgtgga acagtgtttt
     5881 ccccgcaatg ccgtctgaaa gcgcggcagt cgtcggagcg gcggtgtttc tcggcgtatc
     5941 gctcaaaatg ccgctgaccg ccattgtgtt cgtattagaa ctgacctacg cgccgctgtc
     6001 cctgctgctg cctttgtgca tcggcatggc gggggcattg ggaacatcgg gaaaaatggg
     6061 cttcaaatga atgtcgacaa atgccgtctg aaaggttttc agacggcatt tctttgtaaa
     6121 ttgtcaacaa tatctgcaaa aatctactgc caaaaaactg aaaatagcgg ataataccgc
     6181 cccgaaaaag ttcccctttc cccatccaac tgtcagaaaa ggaagcccaa tgtccgtcat
     6241 caaacgtgcc ctgatcagcc tgtccgacaa ggcaggcgcg gtcgaatttg cccaaaccct
     6301 gcacaagctc ggtgtcgaaa tcctctccac cggcggcacg gcgaaactgc ttgccgatgc
     6361 gggcgttccc gtgattgaag tcgccgacta taccggcttc cccgaaatgc tcgacggccg
     6421 cgtgaaaacg ctgcatccga aaatccacgg cggtattttg
//
LOCUS       DAWHSK010000106         6434 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.106, whole genome shotgun sequence.
ACCESSION   DAWHSK010000106 DAWHSK010000000
VERSION     DAWHSK010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6434
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.106"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..1624
                     /gene="dinG"
                     /locus_tag="WIC03_001278"
     CDS             <1..1624
                     /gene="dinG"
                     /locus_tag="WIC03_001278"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HEZ3883774.1"
                     /translation="EFSARRFNGDRDTWPEKIDDAIWLKVTNDRHGCLKTACPNRPEC
                     PFYLARDVLETVDVVVANHDLLLADISMGGGVILPAPENSFYCIDEAHHLPKKALSRF
                     AAEHSWNIAVWTLEKLPQLTGKIAALTDKAELANLADEAAASLLDSLHEWQFHLAEEP
                     SLSLGVSENDRRTNSEPTWLWEDGKIPEGLETTVSNTAIAARSLLKHVIGLNDALSAA
                     RREKEQDGALLDRLTGEFGIFIVRIEQISAVWDLLSTVPLEGEEPLAKWITLRADDKN
                     DYIFNASPISSASHLANSLWRRAAGAVLTSATLQSLGNFNLMLRQTGLQWLPETTTLA
                     LKSPFDFEKQGELYIPPIYASPKDPEAHTAAVIEWLPKLISPTEAIGTLVLFSSRKQM
                     QDVALRLPGDYLPLLLVQGELPKAVLLQKHHRAIEEGKASIIFGLDSFAEGLDLPGTA
                     CVQVIIAKLPFAMPDNPIEKTQNRWIEQRGGNPFIEITVPEAGIKLIQAVGRLIRTEQ
                     DYGRVTILDNRIKTQRYGQQLLAGLPPFKRIG"
     gene            1661..2275
                     /locus_tag="WIC03_001279"
     CDS             1661..2275
                     /locus_tag="WIC03_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HEZ3883775.1"
                     /translation="MNFTRLLNQVLSTVQKKGGTFSDSPLNSFGGGALVAGIASMLLN
                     GKNRKTITKIGSTAALGYLAYRGYQMWQQNKGRATVTQSDFQPAGETEETYSRTVLRT
                     MIAAAASDGMIDEAERRTIEQESGTDPETAAWLAAEYRLPASIEDIAAAVGNDEALAA
                     EAYLAARLVCADLSRKETVFLARLSQALELDDNLVENLERQLGF"
     gene            complement(2370..4238)
                     /locus_tag="WIC03_001280"
     CDS             complement(2370..4238)
                     /locus_tag="WIC03_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEZ3883776.1"
                     /translation="MQAVRYRPEIDGLRAVAVLSVMIFHLNDRWLPGGFLGVDIFFVI
                     SGFLITGIILSEIQNGSFSFRGFYTRRIKRIYPAFIAAVSLASVIASQIFLYEDFNQM
                     RKTVELSAVFLSNIYLGFQQGYFDLSADENPVLHIWSLAVEEQYYLLYPLLLIFCCKK
                     TKSLRVLCHISIILFLILTASSFLPSGFYTDILNQPNTYYLSTLRFPELLAGSLLAVY
                     GQTQNGRRQTANGKRQLLSSLCFGALLACLFVIDKHNPFIPGMTLLLPCLLTALLIRS
                     MQYGTLPTRILSASPIVFVGKISYSLYLYHWIFIAFAHYITGDKQLGLPAVSAVAALT
                     AGFSLLSYYLIEQPLRKRKMTFKKAFFCLYLAPSLILVGYNLYAMGILKQEHLRPLPG
                     APLAAENHFPETVLTLGDSHAGHLRGFLDYVGSREGWKAKILSLDSECLVWVDEKLAD
                     NPLCRKYRDEVEKAEAVFIAQFYDLRMGGQPVPRFEAQSFLIPGFPARFRETVKRIAA
                     VKPVYVFANNTSISRSPLREEKLKRFAANQYLRPIQAMGDIGKSNQAVFDLIKDIPNV
                     HWVDAQKYLPKNTVEIYGRYLYGDQDHLTYFGSYYMGREFHKHERLLKSSRDGALQ"
     gene            complement(4318..5688)
                     /gene="purB"
                     /locus_tag="WIC03_001281"
     CDS             complement(4318..5688)
                     /gene="purB"
                     /locus_tag="WIC03_001281"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEZ3883777.1"
                     /translation="MINPIASLSPLDGRYAQSVEALRPIFSEYGLMKARVKVELNWLK
                     ALAAEPKIVEVPPFSAETLAEIDTVIENFSLEDAAAVKAIEATTNHDVKAIEYWLKER
                     FSGVPEVAAASEFIHFACTSEDINNLSHALMLQEAREAVLLPKLAEIIEKLTGMAHEL
                     AAVPMMSRTHGQPATPSTLGKEIANVVYRLQRQFKNLQAQEFLGKINGAVGNYNAHMV
                     AYPDVDWETHCRNFVEISLGLTFNPYTIQIEPHDYMAEFFQAISRVNTILIDFNRDVW
                     GYISLGYFKQKVKAGEVGSSTMPHKVNPIDFENSEGNLGMANAVLGFLSEKLPISRWQ
                     RDLTDSTVLRNMGVGVGYAVLGFAAHLRGLNKLEPNPAALAADLDATWELLAEPIQTV
                     MRRYGVANPYEKLKDLTRGKDGITPEVLKLFIESLEIPAEAKAKLLELTPALYVGKAE
                     ALAKRI"
     gene            complement(5760..6392)
                     /locus_tag="WIC03_001282"
     CDS             complement(5760..6392)
                     /locus_tag="WIC03_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEZ3883778.1"
                     /translation="MENTNRVPEQARYDAERRQADEALAGVFPAVSIFGSARTPQNHA
                     DYAFACRLARRLSDSGIAVISGGGPGIMEAANKGAFAGKSVSVGLNIVLPHEQKPNPY
                     QDIALRFSRFAERKAVFFRYSQAYVVMPGGFGTLDELFEILTLVQTGKVPPRPIVLVG
                     KAFWSGLAEWINAQLLARGLISEGAVSLFAISDDEDEIVAYLSEHGLQTA"
BASE COUNT         1603 a         1834 c         1656 g         1341 t
ORIGIN      
        1 cgaattttcc gcccgccgct ttaatggcga ccgcgacact tggccggaaa aaatcgatga
       61 cgcgatttgg ctcaaagtga ccaacgaccg ccacggctgc ctgaaaaccg cctgtcccaa
      121 ccgtccagaa tgcccttttt acctggcacg cgatgtcttg gaaaccgtcg atgtcgtcgt
      181 tgccaaccac gatcttctgc ttgccgacat cagcatgggc ggcggtgtaa ttctgcctgc
      241 gcccgaaaac agtttctatt gcatcgacga agcgcaccac ctgcccaaaa aagccctcag
      301 ccgttttgcc gccgaacatt catggaatat tgccgtttgg acgctggaaa aactgccgca
      361 gcttaccggc aaaattgccg cgctgaccga taaagccgaa cttgccaacc ttgccgacga
      421 agccgccgca tccttgctcg acagcctgca cgaatggcag ttccatttgg cggaagagcc
      481 gtctttaagt ttgggggtgt ctgaaaacga cagacgaacc aacagcgaac cgacttggct
      541 gtgggaagac ggcaaaatcc ccgaaggcct cgaaaccacc gtttccaata cggccattgc
      601 tgcgcgcagc ctgctcaaac acgttatcgg gctgaacgat gcgctttccg ccgcgcgccg
      661 cgaaaaagaa caggacggcg cgctcctcga ccgcctgacc ggcgagttcg gcatcttcat
      721 cgtccgtatc gaacaaatca gcgcggtttg ggatttgctc tccaccgtcc ccctcgaagg
      781 cgaagagccg ctggcaaaat ggataaccct ccgtgccgac gacaaaaacg actacatttt
      841 caacgccagc cccatcagca gcgcatccca ccttgccaac agcctgtggc ggcgcgcggc
      901 aggcgcggta ttgacttccg ccaccctgca atccttgggc aacttcaacc tgatgctgcg
      961 ccaaaccggg ctgcaatggc tgcccgaaac caccaccctc gccctcaaaa gcccctttga
     1021 ctttgaaaaa cagggcgaac tctacatccc ccccatatac gccagcccca aagaccccga
     1081 agcccacacc gccgccgtca tcgaatggct gcccaagctt atttcgccca ccgaagccat
     1141 cggcacgctc gtcttgtttt cctcgcgcaa acaaatgcag gatgtcgccc tgcgcctgcc
     1201 cggagactac ctgccgctct tgctcgtaca aggcgaatta cccaaagccg tcctcctgca
     1261 aaaacaccac cgggccatag aagaaggcaa agccagcatc atcttcggac tcgacagctt
     1321 tgccgaagga ctcgacctgc ccggcaccgc ctgcgtgcaa gtcatcatcg ccaaacttcc
     1381 cttcgccatg cccgacaacc ccatcgaaaa aacccaaaac cgctggatag aacagcgcgg
     1441 cggcaacccc ttcatcgaaa tcaccgtccc cgaagccggc atcaaactca tccaggccgt
     1501 cggccgcctc atccgcaccg aacaagacta cggccgcgta accatcctcg acaaccgcat
     1561 caaaacacag cggtacggcc aacaattatt ggccggcctg ccgccgttta aaaggatagg
     1621 gtaaacatac cgcccttccc gcaacagaaa ggaaaaattc atgaacttta cccgactgct
     1681 caaccaagtc ttaagcacgg ttcaaaaaaa aggcggcaca ttctccgaca gcccgctcaa
     1741 ttcattcggc ggaggcgcgc tggttgccgg tatcgcctcc atgctgctga acggtaaaaa
     1801 ccgcaaaacc attaccaaaa tcggttcgac cgccgctttg ggctacctcg cctaccgggg
     1861 ctatcagatg tggcagcaaa acaaagggcg ggcaactgta acacaaagcg atttccaacc
     1921 tgccggagaa actgaagaaa catacagccg caccgtattg cgcaccatga tagccgccgc
     1981 cgcttcagac ggcatgatag acgaagccga acgccggact atcgaacagg aaagcggcac
     2041 agaccccgaa actgctgcat ggctcgccgc cgaataccgc ctgcccgcaa gcatcgaaga
     2101 catcgccgcc gccgtcggca acgatgaggc gttggcggcg gaggcctatc tggcggcaag
     2161 actggtatgt gccgatttgt cgcggaaaga aaccgtcttc ctcgcccgcc tgtcgcaggc
     2221 tttggaactg gatgacaatc tggtggaaaa cttggaaagg caattggggt tttgattcaa
     2281 aattccctcc cgcaggggaa ggaaaataaa aaccgccgcc gccccgaaag gcaaacggcg
     2341 gcacaaacga tatcggacgg caaggcaggc tactgcaatg cgccgtcgcg agaagattta
     2401 agcaggcgtt cgtgtttgtg aaattcccgc cccatataat aagaaccgaa ataggtcagg
     2461 tggtcttggt cgccgtaaag atagcggccg tatatttcga ccgtgttttt gggcaggtat
     2521 ttttgtgcgt ccacccaatg cacattggga atatctttaa tcaaatcaaa gaccgcctga
     2581 ttgctcttgc cgatgtcgcc catagcctga atggggcgga gatattggtt tgcggcaaat
     2641 cttttcaatt tttcctccct caggggcgaa cggctgattg atgtgttgtt tgcaaaaaca
     2701 tagacgggtt tgacggcggc tatccttttg acggtttccc tgaatcgggc tgggaacccg
     2761 ggtattagga aggattgcgc ttcaaatctc ggcacgggct ggccgcccat cctcaaatca
     2821 tagaattggg caatgaaaac ggcttcggct ttttcaactt catcccggta ttttcgacat
     2881 aacgggttgt ctgccagctt ctcatctacc caaaccaaac actccgaatc gagggacagg
     2941 attttggctt tccacccttc ccggctgccg acataatcca aaaacccccg caggtgtccg
     3001 gcgtgcgagt cgccgagggt caggacggtt tccggaaaat gattttccgc agcaaggggc
     3061 gcgccgggca acgggcggag gtgttcctgt ttcaatatcc ccattgcgta caggttgtaa
     3121 ccgacaagta tcagggacgg ggcgagatag aggcagaaaa atgccttttt gaaggtcatc
     3181 ttccgttttc taagcggctg ttcaatcaaa taataactca acagggaaaa tccggccgtc
     3241 aacgcggcaa ccgccgatac ggcaggcagt ccgagctgtt tgtcgcctgt aatgtaatgg
     3301 gcgaaagcaa taaaaatcca atggtacagg tatagggaat aagagatttt gccgacaaat
     3361 acgatggggc ttgccgacag gatgcgggtc ggaagtgtcc cgtattgcat actccggata
     3421 agcagtgccg tcagcaggca gggaaggagc agggtcattc ccgggataaa cggattgtgt
     3481 ttgtcaatca cgaacaggca ggcaagcaat gcgccgaagc agagtgatga aagcaactgc
     3541 cgttttccat ttgctgtttg ccgtctgccg ttttgcgttt gcccgtaaac cgccagcagc
     3601 gaacctgcca acagctcggg aaacctcagt gtcgaaaggt aataagtatt gggttggttg
     3661 aggatgtcgg tataaaaccc gcttggcaaa aacgatgagg cagtcaaaat caaaaacagg
     3721 atgatgctga tgtggcacaa cacccgtagc gatttggttt ttttgcagca aaatatcagc
     3781 aaaagaggat acaggaggta atactgttcc tctaccgcca aagaccagat atgcagtacg
     3841 gggttctcgt cggcactcaa atcgaaatac ccctgctgaa accccagata aatattggac
     3901 aagaaaaccg cagaaagctc cacggttttc cgcatttggt tgaaatcttc gtaaaggaag
     3961 atttgagagg caatcaccga agccagggac acagccgcaa taaaggcagg gtaaatccgc
     4021 ttaatcctgc gggtataaaa accccggaaa gaaaaagaac cgttctgtat ttcagaaaga
     4081 atgatgccgg taatgaggaa tcccgagatg acaaagaaaa tgtccacccc caaaaaccct
     4141 ccgggcagcc agcggtcatt caggtggaaa atcatgacgg atagcacggc gacggcccgc
     4201 aatccgtcaa tttcaggcct gtatcggaca gcttgcataa atatcgcccc gtatgtcccg
     4261 ttatcttaaa aatgccgtct gaacgcgcgt tcagacggca tcggtttcag taaacgctca
     4321 aatccgtttc gccaacgctt cagccttacc cacatacagt gcgggggtca gctcaagcaa
     4381 tttggctttg gcttctgccg gaatttccaa cgactcgata aagagtttca acacttcagg
     4441 cgtaatgccg tctttgccgc gcgtcaggtc tttcagcttc tcataaggat tggctacgcc
     4501 gtaacgacgc attacggttt gaatcggctc ggcgagcagc tcccaagtag cgtccaaatc
     4561 ggcggcaagc gcggcagggt tgggttcgag cttgttcaga ccgcgcagat gggcggcgaa
     4621 acccaacacg gcatagccca cgcctacgcc catattgcgc aatacggtgc tgtcggtcag
     4681 gtcgcgctgc caacgggaaa tcggcagttt ttcggataag aagcccaata cggcgtttgc
     4741 cataccgagg ttgccttcgg agttttcaaa gtcgatcggg ttgactttgt gcggcatggt
     4801 ggaagaaccg acttcgcctg ctttgacttt ttgtttgaag taacccaaag aaatatagcc
     4861 ccaaacatcg cggttgaagt cgatcagaat cgtgttgaca cggctgatgg cttggaagaa
     4921 ttccgccata tagtcgtgcg gctcgatttg aatggtgtag gggttgaagg tcaggccgag
     4981 gctgatttca acgaagttgc ggcaatgggt ttcccaatcc acatcgggat aagcgaccat
     5041 atgggcattg tagttgccga ctgcgccatt gattttgccg aggaactctt gcgcttgcag
     5101 gtttttaaac tggcgttgca ggcggtacac gacattggcg atttctttac ccagtgtgct
     5161 tggtgtagca ggctggccgt gggtgcggct catcatcggg acggcagcca actcatgcgc
     5221 catgccggtc agtttttcga tgatttcggc cagtttcggc agcaaaacag cctcacgcgc
     5281 ttcttgcagc attaaagcgt gggacaggtt gttgatgtct tcgctggtgc aggcaaagtg
     5341 gatgaactcg ctggcagctg ccacttcagg cacgccggag aagcgttctt taagccaata
     5401 ttcaatggct ttgacatcat gattggtcgt ggcttcgatg gctttgacag cggctgcgtc
     5461 ttccaatgaa aagttttcaa tcaccgtgtc gatttcggca agcgtttcgg cactgaaggg
     5521 cggcacttcg acaatcttcg gctcggcggc cagggctttg agccagttta attcgacttt
     5581 gacgcgcgcc ttcatcaggc cgtactctga aaaaatcggg cgcaatgctt caacggattg
     5641 ggcataacgg ccatctaaag gggaaagcga ggcgatgggg ttgatcattt cggcatcctg
     5701 ttcgggaaag acatcaaaaa ttgttaaatt gtttgcaatt atacatcact ctcaggacgc
     5761 tatgccgtct gaagcccgtg ttccgacaga tacgcaacga tttcgtcttc atcgtccgat
     5821 atggcaaaca aagagaccgc cccttcggaa atcagaccgc gcgccaaaag ctgcgcgttt
     5881 atccactccg ccaagcccga ccaaaacgcc tttccgacca aaacaatcgg acgcggcggc
     5941 actttgcccg tctgcaccaa ggtcaggatt tcaaacaatt cgtccagcgt cccgaagccg
     6001 cccggcatca cgacatatgc ttgggaatag cggaaaaaca ccgccttgcg ttcggcaaaa
     6061 cgggaaaacc gcaaggcgat gtcctgatac ggattcggtt tctgctcgtg cggcaaaacg
     6121 atgttcagcc ccaccgaaac cgacttccct gcaaacgcgc ccttgtttgc cgcctccata
     6181 atccccggcc cgccgcccga aatgacggca atgcccgaat ccgacagccg ccgcgccaga
     6241 cggcaggcga acgcataatc cgcatgattc tgcggcgtgc gcgcgctgcc gaagatactg
     6301 actgccggaa acacgcccgc caatgcttcg tctgcctgcc tgcgttcggc atcataacgt
     6361 gcctgctccg gcacacggtt tgtattctcc attccatcct ccgttcaaaa acagcgattg
     6421 tacaccgtca aaaa
//
LOCUS       DAWHSK010000107         6423 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.107, whole genome shotgun sequence.
ACCESSION   DAWHSK010000107 DAWHSK010000000
VERSION     DAWHSK010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6423
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.107"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            110..1651
                     /locus_tag="WIC03_001283"
     CDS             110..1651
                     /locus_tag="WIC03_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEZ3883779.1"
                     /translation="MDGRRWAVWGAFALLPSAFLAVMVVAPLWAMAAYDGLAWGAVLS
                     DAYMLKRLAWTVFQAAATCVLVLPLGVPVAWVLARLAFPGRTFVLRLLMLPFVMPTLV
                     AGVGVLALFGADGLLWRGRQDTPYLLLYGNVFFNLPVLVRAAYQGFVQVPAARLQTAR
                     TLGAGAWRRFWDIEMPVLRPWLAGGVCLVFLYCFSGFGLALLLGGSRYATVEVEIYQL
                     VMFELDMAVASVLVWLVLGVTAAAGLLYAWFGRRAVSDKAVSPVMPSPPQSVGEYVLL
                     AFAAAVLSVCCLFPLLAIVVKAWSAGESWRVLMESETWQAVWNTLRFSAAAVYAAAVL
                     GVVYAAAARRSAWMRGLMFLPFMVSPVCVSAGVLLLYPQWTASLPLLLAMYALLAYPF
                     VAKDVLSAWDALPPDYGRAAAGLGANGFQTACRITFPLLKPALRRGLTLAAATCVGEF
                     AATLFLSRPEWQTLTTLIYAYLGRAGEDNYARAMVLTLLLAAFALGIFLLLDGGEGGK
                     RTETL"
     gene            1680..2279
                     /locus_tag="WIC03_001284"
     CDS             1680..2279
                     /locus_tag="WIC03_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEZ3883780.1"
                     /translation="MSELDNILAHNRQFVESGEYEKYFTDKYPERGLAVLSCMDARII
                     GLLPDALGLKNGDAKLIKNAGALVTHPWGSVMRSLLVAVFELKVREIMVIAHHDCGMQ
                     GLNAEEFLGRVRESRIPEDRIETLRYAGIDLDGWLTGFDNVEDSVRHTVDLIRNHPLM
                     PRHIAVHGLVIHPVTGKLTLVVDGSVSDGMDLSEGMETS"
     gene            2276..2869
                     /gene="nadD"
                     /locus_tag="WIC03_001285"
     CDS             2276..2869
                     /gene="nadD"
                     /locus_tag="WIC03_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HEZ3883781.1"
                     /translation="MKKIGLFGGTFDPIHNGHLHIARAFADEIGLDAVVFLPTGGPYH
                     KDAASASAADRLAMVELATAEDARFAVSDCDIVREGATYTFDTVQIFRQQFPSAQLWW
                     LMGSDSLMKLHTWKKWQMLVRETNIAVAMRQGDSLHQTPRELHAWLGKSLQDGSVRIL
                     SAPMHNVSSTEIRRAGVSDGIPPAAARYIREHGLYEK"
     gene            2928..3314
                     /gene="rsfS"
                     /locus_tag="WIC03_001286"
     CDS             2928..3314
                     /gene="rsfS"
                     /locus_tag="WIC03_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEZ3883782.1"
                     /translation="MNEQELQDLQKMVGVAVNALEDIKAKDISVLETQDKTSLFARMI
                     IASGDSTRQVKALANNVAVDLKEAGFEILSTEGDSGEWTLVDAGDLVVHVMLPAVRDF
                     YDIDTLWGGEKPSFHAGMQKPWHAAD"
     gene            3368..3838
                     /gene="rlmH"
                     /locus_tag="WIC03_001287"
     CDS             3368..3838
                     /gene="rlmH"
                     /locus_tag="WIC03_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEZ3883783.1"
                     /translation="MNITVLAVGTKMPRWVDEAVAEYAKRFGRDVAYVLKEIKPEKRG
                     AGVNAAQGMAAEEKRILEAIPQGAFLVVLDERGKAPTSVELAEHLKTWQQNGEHVCFV
                     IGGADGMTDRLKQQARMMMRLSSLTLPHGMVRVLLTEQLYRAASILHNHPYHRE"
     gene            3913..4179
                     /locus_tag="WIC03_001288"
     CDS             3913..4179
                     /locus_tag="WIC03_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HEZ3883784.1"
                     /translation="MARMVFCVKLNKEAEGMKFPPLPNELGKRIFENVSQEAWAAWTR
                     HQTMLINENRLSLADPRAREYLAQQMEQYFFGDGADAVQGYVPQ"
     gene            4336..5019
                     /locus_tag="WIC03_001289"
     CDS             4336..5019
                     /locus_tag="WIC03_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HEZ3883785.1"
                     /translation="MQHDVYDYTAHTVSKNTVLQKTYRLLGFSFIPAAAGAALAANAG
                     FNFYAAFGSRWIGFAVVLAFFYGMIHFIEKNRYSNTGVTLLMVFTFGMGVLIGPVLQY
                     ALHIADGAKIVGIAAAMTAAVFLTMSALARRTRLDMNALGRFLTVGAVILMVAVVANL
                     FLGIPALALTISAGFVLFSSLMIMWQVRTVIDGGEDSHISAALTLFISLYNIFSSLLN
                     ILLSLNGED"
     gene            5143..5655
                     /gene="coaD"
                     /locus_tag="WIC03_001290"
     CDS             5143..5655
                     /gene="coaD"
                     /locus_tag="WIC03_001290"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEZ3883786.1"
                     /translation="MLPNTPRRAVYAGSFDPPTLGHLWMIRQAQSMFDELIVAIGINP
                     DKRSTYTVAERQDMLCDITKMFPNVRTDVFENRFLVHYAREVDAGFIVRGIRSASDYE
                     YERSMRHINSDLAPEISTVFLMPPREIAEVSSTMVKGLVGPEGWTETIHRYVPQAVYE
                     KILAEHQHEN"
     gene            5687..6340
                     /locus_tag="WIC03_001291"
     CDS             5687..6340
                     /locus_tag="WIC03_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01621 family pseudouridine synthase"
                     /protein_id="HEZ3883787.1"
                     /translation="MLEILFRHQDFVAINKPGGISVHQDSGETGLARTLAIQLGVERV
                     WLLHRLDKQTSGILLFALNRESASALSGQFAGKSIKKTYLALSDRKPSKKQGWIKGGM
                     EKSRRGMWKLTRNTENIAVTRFHSISIAEKLRLFILEPHTGKTHQLRVAMKSLGSPIL
                     GDSLYGGTESETMFLYAWKIQFAYQNRQIEIVAPLKNEWQTENISHALEEFYMAKAG"
BASE COUNT         1401 a         1705 c         1854 g         1463 t
ORIGIN      
        1 ttcagacggc gggaggaagg cggatacggt atgtagtcgg attgaaatat acggtatgtg
       61 ggggcttttg ggtacaatcg caacatcagt gtttcaagtg gggaaggcga tggatggacg
      121 gcgttgggcg gtatggggtg cttttgccct gctgccttcg gcttttttgg cggtaatggt
      181 cgttgcgcct ttgtgggcga tggcggcata tgacggtttg gcgtggggcg cggtgctgtc
      241 ggatgcctat atgctcaaac gtttggcgtg gacggtgttt caggcggcgg caacctgtgt
      301 gctggtgctg cctttgggcg tacctgtcgc gtgggtgctg gcgcggctgg cgttcccggg
      361 gcggactttc gtgctgcgcc tgctgatgct gccgtttgtg atgcccacgt tggtggcggg
      421 cgtgggcgtg ctggctctgt tcggggcgga cggcctgttg tggcgcggac ggcaggatac
      481 gccgtatctg ttgttgtacg gcaatgtgtt tttcaacctt cctgtgttgg tcagggcggc
      541 gtatcagggg tttgtgcaag tgcctgcggc acggcttcag acggcacgga cgttgggcgc
      601 gggggcgtgg cggcggtttt gggacattga aatgcccgtt ttgcgcccgt ggcttgccgg
      661 cggcgtgtgc cttgtcttcc tgtattgttt ttcggggttc gggctggcat tgctgctggg
      721 cggcagccgt tatgccacgg tcgaagtgga aatttaccag ttggtcatgt tcgaactcga
      781 tatggcggtt gcttcggtgc tggtgtggct ggtgttgggg gtaacggcgg cggcagggtt
      841 gctgtatgcg tggttcggca ggcgcgcggt ttcggataag gcggtttccc ctgtgatgcc
      901 gtcgccgccg cagtcggtcg gggaatatgt gctgctggcg tttgcggcgg cggtgttgtc
      961 tgtgtgctgc ctgtttcctt tgttggcaat tgttgtgaaa gcgtggtcgg ccggcgaatc
     1021 gtggcgtgtg ttaatggaaa gtgaaacgtg gcaggcggtg tggaatactt tgcgcttctc
     1081 ggcggcggcg gtgtatgcgg cggcggtttt gggtgtggtg tatgcggcgg cggcgcggcg
     1141 gtcggcgtgg atgcgcgggc tgatgttttt gccgtttatg gtgtcgccgg tttgtgtttc
     1201 ggcgggcgtg ctgctgcttt atccgcagtg gacggcttcg ttgccgctgc tgctggcgat
     1261 gtatgcgctg ctggcgtatc cgtttgtggc aaaagatgtt ttatcagcct gggatgcact
     1321 gccgccggat tacggcaggg cggcggcggg tttgggtgca aacggctttc agacggcatg
     1381 ccgcatcacg ttccccctct tgaaaccggc gttgcggcgc ggtctgactt tggcggcggc
     1441 aacctgcgtg ggcgaatttg cggcaacctt gttcctgtcg cgtcccgagt ggcagacgct
     1501 gacgactttg atttatgcct atttgggacg cgcgggtgag gataattacg cgcgggcgat
     1561 ggtgctgaca ttgctgttgg cggcgttcgc gctgggtatt ttcctgctgt tggacggcgg
     1621 cgaaggcgga aaacggacgg aaacgttata atgcgaaccc tttttcagag gacggcaaaa
     1681 tgagcgagtt ggataacatc cttgcccata accggcagtt tgtcgagtcg ggcgaatatg
     1741 aaaaatactt taccgacaaa taccccgaac gcgggctggc agttttgtcc tgtatggatg
     1801 cgcggattat cgggctgctg cccgacgcgt tgggtttgaa aaacggcgat gccaagctga
     1861 ttaaaaatgc cggcgcgctg gttacgcacc cgtggggttc ggtgatgcgg agtcttttgg
     1921 ttgccgtgtt tgaattgaag gtcagagaga ttatggtcat cgcccatcac gattgcggta
     1981 tgcaggggct gaatgccgaa gaattcctcg ggcgcgtccg ggaaagccgg attcccgaag
     2041 accgtatcga aaccctgcgt tatgccggta tcgacctcga cggctggctg accggtttcg
     2101 acaacgtcga agacagcgtg cgccacacgg tggaccttat ccgtaaccat ccgctgatgc
     2161 cgcgccatat cgccgttcac ggactggtca tccatcccgt taccggcaaa ctgacgctgg
     2221 ttgtggacgg cagtgtttca gacggcatgg acttatcgga aggaatggaa acatcatgaa
     2281 gaaaatcgga ctgttcggcg gcactttcga cccgatacac aacgggcatc tccacatcgc
     2341 ccgcgccttt gccgacgaaa tcggcttgga cgcggttgtt ttcctgccga caggcggccc
     2401 gtatcacaaa gacgcagcct ccgcttccgc cgccgaccgc cttgccatgg tcgaactggc
     2461 gacggcagaa gacgcgcgtt ttgccgtcag cgattgcgac atcgtccgag aaggtgcaac
     2521 ctatactttt gataccgtcc aaatcttccg ccagcagttc ccatccgcgc aactttggtg
     2581 gctgatgggc agcgacagcc tgatgaagct gcacacatgg aaaaaatggc agatgctcgt
     2641 gcgcgaaacc aatatcgccg tcgccatgcg gcagggcgac agcctgcacc aaaccccgcg
     2701 cgaactgcac gcgtggctgg gcaagtccct tcaggacggc agcgtccgca tcttgtccgc
     2761 cccgatgcat aatgtgtcgt caacggaaat ccgccgcgcc ggtgtttcag acggcatccc
     2821 acctgccgcc gcacgctaca tccgcgaaca cggtttgtat gaaaaataaa gtcaaatcag
     2881 taagaaacgg ctataatgcc gtctgaaaac atcccgttag gaaaataatg aacgaacaag
     2941 aactgcaaga cctgcaaaaa atggtcgggg tcgccgtcaa cgccctcgaa gacatcaaag
     3001 ccaaagacat ttccgttctc gaaacgcaag acaaaacttc gctgtttgcc agaatgatta
     3061 tcgccagcgg cgacagtacg cgccaagtca aagcactggc caacaacgtt gccgtcgatt
     3121 tgaaagaagc cggttttgaa atcctcagca ccgaaggcga cagcggcgaa tggacgctcg
     3181 ttgatgcagg agacctcgtc gtccacgtta tgctccctgc cgtgcgcgac ttctatgata
     3241 ttgacaccct ttggggtggc gagaaaccga gttttcacgc cggaatgcag aagccgtggc
     3301 acgcggcaga ctgattcccg atgccgtctg aatgttcaga cggcattttc ctgtaaggga
     3361 gaaagcattg aacatcaccg ttttggcagt cggcaccaaa atgccgcgct gggttgatga
     3421 ggccgtcgcc gaatacgcca aacgcttcgg acgcgacgtc gcctacgtac tcaaagaaat
     3481 caaacccgaa aaacgcggcg cgggcgtgaa tgccgcccaa ggaatggcgg cggaagaaaa
     3541 acgcatcctc gaagccatac cgcaaggcgc gttcctcgtc gtccttgacg aacgcggcaa
     3601 agcaccgacc tccgtcgagc tggcggaaca cctcaaaact tggcagcaaa acggcgaaca
     3661 cgtctgcttc gtcatcggcg gcgcggacgg catgaccgac cgcctcaaac agcaggcccg
     3721 catgatgatg cgcctgtcca gcctgaccct gccgcacggg atggtgcgcg tccttctgac
     3781 cgaacaactc taccgcgccg cctccatcct gcacaaccac ccctaccatc gggaataaga
     3841 aggcttttgt ccgcactccc aatcggttaa aatccccccg ttatttctga acacacagca
     3901 aaggaatccg ctatggcacg catggtattc tgcgtcaagc tcaacaaaga agccgaaggc
     3961 atgaaatttc cgccgcttcc caacgaattg ggcaagcgca tttttgaaaa cgtatcgcaa
     4021 gaagcgtggg cggcgtggac gcgccaccaa acgatgctga ttaacgaaaa ccgtttaagc
     4081 ctcgccgatc cgcgcgcgcg cgaatacctg gctcagcaga tggagcagta tttcttcggc
     4141 gacggcgcgg atgccgttca gggatacgtt ccgcaataac ggttttccgt tttgaacaca
     4201 ggctgtccga aactgcttca gacggccttt aaaatacgcc ggacaacttt atcttcacgc
     4261 aaccggacaa attttgacat tgggcaatgt taaaatcccc cattattccc aaccgcgctt
     4321 tcaggagcag atgatatgca acacgacgtt tacgactaca ccgcgcatac ggtttctaaa
     4381 aacaccgtcc tgcagaaaac ctaccgcctg ctcggatttt cattcattcc ggcagccgca
     4441 ggcgcggcac ttgccgccaa tgccggtttc aatttttacg ccgccttcgg ttcgcgctgg
     4501 atagggtttg ccgtcgtgtt ggcgtttttc tacggtatga tccacttcat cgagaaaaac
     4561 cgttacagca ataccggcgt taccctgctg atggtattca cattcggtat gggcgtattg
     4621 atcggccccg tgctgcaata cgcgctccat attgccgacg gtgcgaaaat cgtcggcatt
     4681 gccgccgcga tgaccgccgc cgtcttttta acgatgtccg ccttggcgcg ccgaacccgg
     4741 ctcgatatga acgcgctcgg acgcttcctg accgtaggtg cggtcattct gatggtcgcc
     4801 gtggttgcca atctgttttt gggtattccc gcactcgccc tgaccatttc cgccggtttt
     4861 gtcttgttca gttccttaat gattatgtgg caggtacgca ccgtcatcga cggcggcgaa
     4921 gacagccaca tcagcgcggc actgacactg tttatctcgc tttacaacat cttcagcagc
     4981 ctgctgaaca tcctgttgtc tttaaacggc gaggattgat gccggtaaaa cgccgtccga
     5041 ctatgccgtc tgaaaatgct tcagacggca ttttactttg ggcatacaat acggatgcac
     5101 acgcaccaac acacagccga ctatgaagcc gggaggaatc aaatgttacc gaatacaccg
     5161 cgccgcgccg tttatgccgg cagtttcgat ccgcccacat tggggcatct gtggatgata
     5221 cggcaggcgc aatctatgtt tgacgaactc atcgtcgcca tcggcattaa ccccgacaaa
     5281 cgcagcacct ataccgtcgc tgaaaggcag gatatgttgt gcgatattac taaaatgttt
     5341 cccaacgtca gaaccgatgt atttgaaaac cgatttctgg tgcattacgc ccgtgaggta
     5401 gatgcaggat tcatcgtgcg cggcatccgt tctgcttcgg attacgaata cgaacgttcc
     5461 atgcgccata tcaacagcga cctcgccccc gaaatatcca ccgtattcct catgccgccg
     5521 cgcgaaatcg ccgaagtgtc gtccactatg gtcaaaggac tggtcgggcc ggaaggctgg
     5581 acggaaacga tccaccgcta cgtgccgcaa gctgtgtacg aaaaaatcct tgccgaacat
     5641 caacacgaaa attgaaccct tctatttttg caaggcatat atggatatgt tggaaatttt
     5701 gttccgccat caagacttcg tcgccatcaa caaaccgggc ggcatatccg tccaccaaga
     5761 cagcggcgag accggactgg cccgaacact cgccatacag ttgggcgtag agcgtgtgtg
     5821 gctgctgcac cgattggaca aacagaccag cggcatttta ctgtttgccc tcaacagaga
     5881 aagcgcgtcc gcccttagcg gacagtttgc cggcaaaagc atcaaaaaaa cctatttggc
     5941 actatccgac cgcaaaccgt ccaaaaagca gggttggatt aaaggcggaa tggaaaaatc
     6001 cagacgcgga atgtggaagt tgacgcgcaa tacagaaaat atcgccgtta cccgattcca
     6061 cagcatcagt atcgccgaaa aactgcggct gttcatcctg gaaccgcata cgggcaaaac
     6121 gcaccaattg agagtggcga tgaaaagttt gggcagtccg attctgggcg acagcttata
     6181 cggcggaaca gaatccgaaa ccatgtttct gtatgcatgg aaaatacaat ttgcctacca
     6241 aaaccgacaa atcgaaattg ttgcaccctt aaaaaacgaa tggcagactg aaaatatctc
     6301 tcatgcgctg gaagaatttt atatggcaaa agcaggttaa taaatataaa ttaatttata
     6361 aacaatatga taaatatttt tataaaaatt tttcaagaaa acggttgact gcaaatctct
     6421 tca
//
LOCUS       DAWHSK010000108         6349 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.108, whole genome shotgun sequence.
ACCESSION   DAWHSK010000108 DAWHSK010000000
VERSION     DAWHSK010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6349
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.108"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            203..1624
                     /locus_tag="WIC03_001292"
     CDS             203..1624
                     /locus_tag="WIC03_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HEZ3883788.1"
                     /translation="MDTKEILGYAAGSIGSAVLAVIILPLLSWYFPTDDIGRIVLMQT
                     AAGLTVSALCLGLDQAYVREYYAAADKDTLFKTLFLPPLLSAAAIAVLLLSRPTLPSE
                     ILFSLDDAAAGIGLVLFELSFLPIRFLLLVLRMEGRALAFSSAQLVPKLAILLLLPLT
                     VGLLHFPARTAVLTAVYALANLAAAAFLLFQNRCRLKAVRRAPFSSAVLHRGLRYGIP
                     IALSSIAYWGLASADRLFLKKYAGLEQLGVYSMGISFGGAALLFQSIFSTVWTPYIFR
                     AIEENAPPARLSATAESAAALLASALCLTGIFSPLASLLLPENYAAVRFIVVSCMLPP
                     LFCTLVEISGIGLNVVRKTRPIALATLGALAANLLLLGLAVPSGGARGAAVACAASFW
                     LFFVFKTESSCRLWQPLKRLPLYMHTLFCLASSAAYTCFGTPANYPLFAGIWAAYLAG
                     CLLRHRKDLHKLLHYLKKQGFPL"
     gene            1621..2692
                     /locus_tag="WIC03_001293"
                     /pseudo
     CDS             1621..2692
                     /locus_tag="WIC03_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258336.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
     gene            2706..3869
                     /locus_tag="WIC03_001294"
     CDS             2706..3869
                     /locus_tag="WIC03_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ3883789.1"
                     /translation="MNITIVAPYCSLPSEPYFNRFWYLAERLSQSHDVLLITSRFRHY
                     DKSFRRHEDAEAASQGRLKVMLLEESGYGKNVSLERVASHYRFVKNFEQWLKNCRPGE
                     QDIVYSAYPLIATNLLLGKHKARLGYKLIVDVQDVWPESFSSVVPFLKKVPHKLLPFA
                     SRANRAYRCADALVAVSQTYLDRAKEANPNVPGEAVYIGTDFAAIAPPRFRSKTVRLF
                     YLGTLSYSYDVETVCKGVRKLLDDGENVELHVMGGGPDLEKLKQYENRAIKFYGYLPY
                     SEMMSIAKACDIAVNAIHSHAMQSVTNKLSDYMALQKPILNSQTNAEVLDLMNLLPHE
                     NYRSGDVDGFVQAAKNILKRKNDSVQSDEIVRRFRRDVAYQKIVNLIERLAHE"
     gene            3862..5439
                     /locus_tag="WIC03_001295"
     CDS             3862..5439
                     /locus_tag="WIC03_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEZ3883790.1"
                     /translation="MSKFFKRLFDIVASASGLIFLSPVFLILIYLIRKNLGSPVFFIQ
                     ERPGKDGKPFKMIKFRSMRDALDSDGIPLPDGERLTPFGKKLRAASLDELPELWNVLK
                     GEMSLVGPRPLLTGYLPLYNDFQNRRHEMKPGITGWAQVNGRNALSWDEKFACDVWYT
                     DNFSFWLDMKILFLTVKKVLIKEGISAEGEATMPYFTGNQTNEKNNILILSAGRRVEL
                     VQDFQTEAARFSDGISVFATDLNPRMSSACHVSDGSFEVPPISADSYIDSIFNLAVRE
                     NIGLIIPTIDTELQKLADERERFEAASVHIVVSDSDFIAQCRDKRKTATLFARYGIRS
                     PEIYDRKKLVFPCFAKPYDGSRAIGAKRIDTPADLTPEVLADTKLMFCQFIDIENKFS
                     EFTVDMYYDRQCRLKCAIPRKRLEVRSGEISKGVTCKNSLYQTLLEKMSVLQGARGCI
                     TAQFFYNEETGEFYGVEINPRFGGGFPLTYAAGGNYPSRLMREYLGDEEIPFSDDWEN
                     NLIMLRYDAKVLVHEND"
     gene            5426..6112
                     /locus_tag="WIC03_001296"
     CDS             5426..6112
                     /locus_tag="WIC03_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ3883791.1"
                     /translation="MKTINPETTVIVFDLDDTLYSEYEYKLSGIRSVIDTVAALYPDW
                     NSDNLWRNIDPDGKDWLDKLCRHCGFNESEKQVLLWQYRLHRPTLTPYAPPDFLSELT
                     APFAARALITDGRSLTQRLKLEALGLSSLFDDILISEACSSEKPDGKRFRHLQDKYAD
                     KAGCFIYIGDNISKDFIAPNTLGWITIGLLPSGQNIHRHQPESFNTEYHPDFWVNSLQ
                     DLVPIIDSNT"
BASE COUNT         1449 a         1927 c         1563 g         1410 t
ORIGIN      
        1 agccaacgcc gtactggttt ttgttaatcc actataacgc cgataaaaaa cggccccgtt
       61 cagaccgccg cccgcccgga aaagcgcaac ccggactgcc gcaccccgcc ggcagccgcg
      121 acggtctgaa agccgtcaaa ttccaatcaa gaaaggcttc agacggcaca ggcagcatcc
      181 cgcctccgtc cggacatcaa aaatggacac aaaagaaatc ctcggctacg cggcaggctc
      241 gatcggcagc gcggttttag ccgtcatcat cttgccgctg ctgtcgtggt atttccccac
      301 cgacgacatc gggcgcatcg tgctgatgca gacggcggcg ggactgacgg tgtcggcatt
      361 gtgcctcggg ctggatcagg catacgtccg cgaatactat gccgccgccg acaaagacac
      421 tttgttcaaa accctgttcc tgccgccgct gctgtctgcc gccgcgatag ccgtcctgct
      481 gctttcccgc ccgaccctgc cgtctgaaat cctgttttcg ctcgacgatg ccgccgccgg
      541 catcgggctg gtgctgtttg aactgagctt cctgcccatc cgctttctct tactggtttt
      601 gcgtatggaa gggcgcgccc ttgccttttc gtccgcgcaa ctcgtgccca agctcgccat
      661 cctgctgctg ctgccgctga cggtcgggct gctgcacttt ccggcgcgca ccgccgtcct
      721 gaccgccgtt tacgcgctgg caaaccttgc cgccgccgcc tttttgctgt ttcaaaaccg
      781 atgccgtctg aaggccgtcc ggcgcgcacc gttttcatcc gccgtcctgc atcgcggcct
      841 gcgctacggc ataccgatcg cactaagcag catcgcctat tgggggctgg catccgccga
      901 ccgtttgttc ctgaaaaaat atgccggcct agaacagctc ggcgtttatt cgatgggtat
      961 ttcgttcggc ggggcggcat tattgttcca aagcatcttt tcaacggtct ggacaccgta
     1021 tattttccgc gcaatcgaag aaaacgcccc gcccgcccgt ctctcggcaa cggcagaatc
     1081 cgccgccgcc ctgcttgcct ccgccctctg cctgaccggc attttctcgc ccctcgcctc
     1141 cctcctgctg ccggaaaact acgccgccgt ccggtttatc gtcgtatcgt gtatgctgcc
     1201 gccgctgttt tgcacgctgg tagaaatcag cggcatcggt ttgaacgtcg tccgaaaaac
     1261 acgcccgatc gcgctcgcca ccttgggcgc gctggcggca aacctgctgc tgctggggct
     1321 tgccgtaccg tccggcggcg cgcgcggcgc ggcggttgcc tgtgccgcct cattttggct
     1381 gttttttgtt ttcaagaccg aaagctcctg ccgcctgtgg cagccgctca aacgcctgcc
     1441 gctttatatg cacacattgt tctgcctggc ctcctcggcg gcctacacct gcttcggcac
     1501 gccggcaaac taccccctgt ttgccggcat atgggcggca tatctggcag gctgcctcct
     1561 gcgccaccgg aaagatttgc acaaactgtt gcattatttg aaaaaacaag gtttcccatt
     1621 atgaaaatcg ttttgaccac atctatggca ggcttgggcg gcacggaaac cgccaccgtc
     1681 cgcctcgggc ggctgctcaa acggcgcggg cacgacatca tccttgcttc ttcggacggg
     1741 ccttttgtcg gagaggcgca agcatcgggc atccgttggc agccggttga tttttaccgg
     1801 ggcgggcttg ccggctacct caaaagcacc tttgcctacg cccggatgct gcgccgcgaa
     1861 caacccgaca tcatcgactg ccaaatggcg cgcgttgttc cagcctgcgc ccttgccgcc
     1921 aaaatcgttt cgccgaaaac caaaatcatc taccactcgc acggtttgga cgcggcaacc
     1981 taccccaaaa tcgccaaact cttcgacaag ctgggcgtgt acatcatcgg taactgcaaa
     2041 cacgagcgcg aaaaactcat ccgccacggc ttccccgccg gccggattgc ctacgcctac
     2101 aacgcactcc ccccccccgg aatttcattt ccggaaaacc gaaaaagaat gcgccgtact
     2161 cggcacgctt tcccgtttgg acaccgtccg cgccgtgcat ctgatgttgg atattttgaa
     2221 gaaaatggtt gaccgcggta tgcccgtccg cctcaacata gcgggcatag gagaagaaat
     2281 ggacaatttg aaaacgcagg cgaagcatct gggcattgaa gacaaagtta ccttcctcgg
     2341 cggcatacgc gatttgaccg cctacttcaa agacgtggat attttagtga acacgcccca
     2401 ctgcatcggc gaccacggcg caggtgtggg caacaacatc ttggaagccg gactttacga
     2461 cacgcccgtc gttacctacg atatggctgg catttccgaa atggtcgtca acggagaaac
     2521 gggttactgc atccccttcg gcgacgaaga agcctttatc gccgccgtag acacgctcgt
     2581 cagacaaccg gagttgcgcg gtcaaatggg caaagccctg cacaaacacg tcgaaacctt
     2641 atgctccgac gacgaaatct accgtaccac aatggctgcg tacgaaatgt aaggctgtcc
     2701 gaaatatgaa catcaccata gtcgccccct actgctcgct gccgtccgaa ccgtatttca
     2761 accgcttttg gtatctggca gaacgattgt cgcaatcgca cgacgtgttg ctgattacca
     2821 gccgtttccg ccactacgac aaatccttca gacggcatga agatgccgag gccgcctcgc
     2881 aaggccgtct gaaagtcatg ctgttggaag aaagcggcta cggcaaaaac gtgtctttag
     2941 aacgtgttgc cagccattac cgcttcgtca aaaattttga gcaatggctg aaaaactgcc
     3001 ggccgggcga acaagacatc gtctattccg cctatccgct gattgccacc aacctgctgt
     3061 tgggcaaaca caaagcgcgt ttgggctaca aactgattgt cgacgtacag gacgtatggc
     3121 cggagtcttt ctcctcggtc gtaccgtttt tgaaaaaagt accgcacaag ctgctgccct
     3181 ttgcttcacg cgccaaccgt gcctaccgct gcgccgacgc gctggttgcc gtatcgcaga
     3241 cttatctcga ccgcgccaaa gaagccaatc cgaacgtacc cggcgaagcc gtctatatcg
     3301 gtacggattt tgccgccatc gcccccccgc gattccgctc caaaaccgtc cgccttttct
     3361 acttgggcac gctcagttac agctatgacg tggaaaccgt gtgcaaaggt gttcgcaagc
     3421 tgttggacga cggcgaaaac gtcgaattgc acgttatggg cggaggaccc gatttggaga
     3481 aactcaaaca atatgaaaat cgcgccatca agttttacgg ttatttaccc tattctgaaa
     3541 tgatgtcgat tgccaaagcg tgcgacattg ccgtcaacgc catccatagc cacgccatgc
     3601 agtcggttac caacaaactc tccgactata tggccttgca aaaaccgatt ctgaacagcc
     3661 agaccaatgc cgaagtcctc gacctcatga atctgctgcc acacgaaaac taccgttccg
     3721 gcgatgtgga cggtttcgtt caagccgcca aaaatatttt gaaacgcaaa aacgattctg
     3781 ttcagtccga cgaaatcgtc cgccgcttca ggcgcgatgt tgcctatcaa aaaatcgtca
     3841 acctgattga aagattagcc catgagtaaa ttcttcaaac gcctgtttga cattgttgcc
     3901 tccgcctcgg gactgatttt cctctcgcca gtatttttga ttttgatata cctcatccgc
     3961 aagaatctgg gttcgcccgt gttcttcatc caagagcggc cgggcaagga cggcaagccg
     4021 tttaaaatga ttaaattccg ctctatgcgc gacgcgcttg attcagacgg cattccgctg
     4081 cccgacggag aacgcctgac accgttcggc aaaaaactgc gtgccgccag cttggacgaa
     4141 ctgcccgaac tgtggaatgt cttaaaaggc gagatgagcc tggtcggccc gcgcccgctt
     4201 ttgacgggct acctgccgct ttataacgac ttccaaaacc gccgccacga aatgaaaccg
     4261 ggcattaccg gctgggcgca ggtcaacggg cgcaacgcgc tttcgtggga cgaaaagttc
     4321 gcctgcgatg tttggtacac cgacaacttc agcttttggc tggatatgaa aatcctgttt
     4381 ttgacagtca aaaaagtctt gatcaaagaa ggcatttcgg cggaagggga agccactatg
     4441 ccttatttca ctggaaatca aactaatgaa aaaaataata tactgattct gtcggcaggt
     4501 cgccgtgtcg aattggtgca ggacttccaa accgaagccg cacgtttttc agacggcatc
     4561 agcgtttttg ccactgacct caacccccgc atgtcttctg cctgccatgt ttcggacggt
     4621 tcatttgaag tgccgccgat tagtgccgat tcatatatcg acagcatttt caatctggcg
     4681 gtacgcgaaa atatcggttt aatcatccca accatagaca ccgaactgca aaagctggca
     4741 gacgagcgcg aacgctttga agcggcaagt gtccacatcg tcgtttccga ttcggacttc
     4801 atcgcacaat gccgcgacaa acggaaaacc gccaccttgt ttgcccgcta cggcatacgc
     4861 tcacccgaaa tttacgaccg caagaagttg gttttccctt gcttcgccaa gccttatgac
     4921 ggcagccgcg ccatcggtgc aaaacggatt gacacacctg ccgacctgac accggaagtc
     4981 ttggcagaca ccaaattaat gttctgtcag tttatcgaca tcgaaaataa attttccgag
     5041 tttaccgtcg atatgtatta cgaccgtcaa tgccgtctga aatgcgccat cccgcgaaaa
     5101 cgcttggaag tgcgctccgg cgaaatcagc aaaggcgtta cctgcaaaaa cagcctctac
     5161 caaacgctgc tggaaaaaat gtcggtattg cagggcgcgc gcggctgtat cacggcgcag
     5221 ttcttctaca acgaagaaac cggcgaattc tacggcgtgg aaatcaaccc ccgcttcggc
     5281 ggtggcttcc cgctgacgta tgccgcaggc ggcaattatc cgagccggtt gatgcgcgaa
     5341 tacctcggcg acgaagaaat cccgttttca gacgactggg aaaacaacct cattatgttg
     5401 cgctacgatg ccaaggtctt ggttcatgaa aacgattaat cctgaaacca ccgtcatcgt
     5461 cttcgacttg gacgataccc tgtattcgga atacgaatac aagctctccg gtatccgttc
     5521 ggtcatcgat accgttgccg cactgtatcc cgattggaat tcagacaacc tatggcgcaa
     5581 catcgacccc gacggcaaag attggctgga caaattgtgc cgccactgtg gttttaatga
     5641 atcggaaaaa caggttcttt tgtggcaata ccggctgcac cgcccgacgc tgactccgta
     5701 tgctccgccg gatttcctgt ccgaactgac cgcgcccttc gccgcacgcg cgctgattac
     5761 cgacggcaga agcctgaccc aacgcctgaa gctggaagcc ttgggtctgt cttccctgtt
     5821 tgacgacatc ctcatctctg aagcctgctc ttcggaaaag cccgacggca aacgcttccg
     5881 ccatcttcaa gacaaatatg ccgacaaggc aggctgcttc atctatatcg gcgacaacat
     5941 ttccaaagac tttatcgcgc cgaacacttt gggctggata accataggat tgcttccctc
     6001 cggacagaac atccaccgcc accagcctga aagttttaac acagaatatc atcccgactt
     6061 ttgggttaac tcccttcaag atttagttcc tataattgat tcaaatactt aaccattaca
     6121 taatcaaaca tgcactcaca atattaaaag gactatttca tgacaaaata ttattcatgg
     6181 ggcgtaaacg gctttccgcc ctacgttttc gacaccattt ggaaacgatg gaagttgaat
     6241 gggcgcgggg gcggttatcg tgtgcgacat cccggacggc atgaccgtcg cgggcaatcc
     6301 ggcaaagccg ctgccgcgca aaaaccccaa gaccgggacg gcataagcg
//
LOCUS       DAWHSK010000109         6340 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.109, whole genome shotgun sequence.
ACCESSION   DAWHSK010000109 DAWHSK010000000
VERSION     DAWHSK010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6340
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.109"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(54..1508)
                     /locus_tag="WIC03_001297"
     CDS             complement(54..1508)
                     /locus_tag="WIC03_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HEZ3883792.1"
                     /translation="MTTQTIKFHMKPETFKQNAAISLQDKPLRKSLRTAMDMLMTKRK
                     AVLTDEEELQSLRDLCEHVRQRSLSKLPALLEQLEENLTKLGVKVHWAETPTEACQII
                     HDIITAKNGKLMVKGKSMVSEEIELNHYLEAKGIKAVESDLGEFIVQMAGEKPTHIVM
                     PAIHKTKEQVSELFHQNLGTPLTDDVDQLTGFARKALRDIYSTADVGLSGVNFAVAET
                     GTLCLVENEGNGRLSTTVPPVHIAITGIEKVVAKLSDIPPLYSLLPRSAIGQNITTYF
                     NMITGPRRSEELDGPQEMHLVLLDNGRSQAYAEDQMRRTLQCIRCGACMNHCPVYTRI
                     GGAAYGTTYPGPIGEIISPHLLGLDATRDLPTACTMCGACVEVCPVRIPITEQMQRLR
                     VEAQRSPTETVPHPIRGQGASHTFGEQMAWRTFNGIFSGSKTYRAFGWAATKFRNLTP
                     RKQLGWTQNRVPMKPAKKTLHELMAEKMRQKEQA"
     gene            complement(1505..2206)
                     /locus_tag="WIC03_001298"
     CDS             complement(1505..2206)
                     /locus_tag="WIC03_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HEZ3883793.1"
                     /translation="MSARENILAKLKKADALPMEEPAVFDYYREMGVSWGSEVERLKH
                     WAAAMRAVKTEIYWVTKSNWMQVFREAAEGKGLKNILLPLATEHGQLARAALAGSNIE
                     PIAFEREIDTWKTEFFANIDAGFSSSQCGIARTGTLMLFSSPEEPRTLSLVPPVHFCL
                     FDTSKMYNEFHNAVEGEKLVENGMPTNVFLISGPSKTADIQLTLAYGAHGPRDLVILA
                     ILPDHISPADLEENA"
     gene            complement(2203..2982)
                     /locus_tag="WIC03_001299"
     CDS             complement(2203..2982)
                     /locus_tag="WIC03_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEZ3883794.1"
                     /translation="MSATIPPKIIRYDSNPTDVYFFGTCVLDLFMPEAGMDAITLIEQ
                     QGIRVHFPMAQSCCGQPAYSSGHPTEAFDVARAQLDLFPEEWPIVVPSGSCGGMMKHH
                     WPTLFKGSEYEERAVDCAGRIIEFTHFLLAIGFKPEDKGEPLKVAVHTSCAARREMNV
                     HLSGWQLIDGMENVERIVHDHESECCGFGGTFSVKQADISGAMVTDKVAALKETGATE
                     IISADCGCMMNIGGKIAKDEPNMPRPKHIASFLLERTGGKA"
     gene            3131..4672
                     /locus_tag="WIC03_001300"
     CDS             3131..4672
                     /locus_tag="WIC03_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEZ3883795.1"
                     /translation="MVFDNNYHYNAIPVRLLVNIQDADSDGIQTASENNNGTRMGYNR
                     SMEKNTLSARAPSPWLPLLLAIAIFMQMLDATILNTALPEIAADLNESPLNMQLAVIS
                     YTLTVALLIPLSGYLADRFGTKKVFFGSIAVFMLGSALCAASGSLFELTLSRVVQGIG
                     GSMLVPIPRLTILRVYDKSKLLNAINYAVMPALIGPVLGPLAGGYLVEYASWHWIFLL
                     NLPIGLLGFILGRNIMPDVKGNDTALDFKGYLIFSAAACLLLLSAESLSHALPPYFAL
                     LPLCGGLLFARRYFRHMKTASKPIYSADLFLIRTFRLGLAGNLFSRLGISSIPFLMPL
                     MFQVAFGFGASLSGWLVAPVALSSLLVKPLIAPLMKRFGYRTVLLWNTKLLAAFIMLL
                     ALPDGNSPLWIWIFLSLAIGACNSLQFSAMNTLTLADLRPQQTGSGNSLMAVNQQLAI
                     SMGIVVGALILKNWTFLIPASSGLHSAFRMTLLSIGGITLASSLVFKRLHVSDGANLT
                     RNTLS"
     gene            4784..6310
                     /locus_tag="WIC03_001301"
     CDS             4784..6310
                     /locus_tag="WIC03_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003765285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HEZ3883796.1"
                     /translation="MKTRSLISLLCLLLCSCSSWLPPLEERTESRHFNTSKPVRLDNI
                     LQIRHTPHTNGLSDIYLLNDPHEAFAARAALIESAEHSLDLQYYIWRNDISGRLLFNL
                     MYLAAERGVRVRLLLDDNNTRGLDDLLLALDSHPNIEVRLFNPFVFRKWRALGYLTDF
                     PRLNRRMHNKSFTADNRATILGGRNIGDEYFKVGEDTVFADLDILATGSVVGEVSHDF
                     DRYWASHSAHNATRIIRSGNIGKGLQALGYNDETSRHALLRYRETVEQSPLYQKIQAG
                     RIDWQSVQTRLISDDPAKGLDRDRRKPPIAGRLQDALKQPEKSVYLVSPYFVPTKSGT
                     DALAKLVQDGIDVTVLTNSLQATDVAAVHSGYVKYRKPLLKAGIKLYELQPNHAVPAT
                     KDKGLTGSSVTSLHAKTFIVDGKRIFIGSFNLDPRSARLNTEMGVVIESPKIAEQMER
                     TLADTTPEYAYRVTLDRHNRLQWHDPATRKTYPNEPEAKLWKRIAAKILSLLPIESLL
                     "
BASE COUNT         1350 a         1835 c         1614 g         1541 t
ORIGIN      
        1 tgccgtctga agccctgttt cgggtttcag acggcatttt tgcgaacaac tttttatgcc
       61 tgttcttttt ggcgcatttt ttctgccatc agttcgtgca gggttttctt cgccggtttc
      121 atcggcacgc ggttttgcgt ccaacccaac tgtttgcgcg gggtcaggtt gcggaacttg
      181 gtggctgccc aaccgaaggc gcggtaggtt ttgctgccgc tgaaaatacc gttgaatgtg
      241 cgccacgcca tttgttcgcc gaaggtatgc gatgcgcctt gcccccggat ggggtgcggc
      301 acggtttcgg tcggcgaacg ttgcgcttca acgcgcaaac gctgcatttg ttcggtaatc
      361 gggatgcgta ccggacaaac ttccacacac gcaccgcaca tggtacaggc ggttggcaga
      421 tcgcgggtgg catccagacc caacaggtga ggggaaataa tctcgccaat cgggccggga
      481 taggttgtgc cgtatgccgc gccgccgatg cgggtataaa ccgggcaatg gttcatacac
      541 gcgccgcaac ggatacattg cagggtgcgg cgcatttggt cttcggcata agcctggctg
      601 cggccgttgt cgagcagaac caagtgcatt tcttgcggac cgtctaattc ttcactgcgg
      661 cgcgggccgg taatcatatt gaaataggtt gtgatgtttt gaccgatggc ggagcgcggc
      721 agcagactgt acaagggtgg gatgtcggac aatttcgcca ccactttttc aatgccggta
      781 atagcgatat gcacgggcgg aacggtggta ctcaagcgac cgttgccctc gttttccacc
      841 agacacagcg tacctgtttc agcaacggca aagtttacgc cactcaagcc gacatcggca
      901 gtgctgtaaa tatcgcgcag ggctttgcgg gcgaagccgg tcagttggtc cacatcgtct
      961 gtcagcggtg tgccgaggtt ttggtggaac agttcgctca cctgttcttt ggttttatgg
     1021 attgcaggca tcacgatatg ggtcggtttt tcgcctgcca tttggacgat gaactcgccc
     1081 aagtcgcttt ctaccgcttt aatgcctttt gcttcaagat aatggttcag ctcgatttcc
     1141 tcgctgacca tggatttgcc tttgaccatc agcttgccgt ttttggctgt gatgatgtcg
     1201 tggataattt ggcaggcttc ggtcggggtt tctgcccagt gcactttcac gcccaactta
     1261 gtcaggtttt cttccagctg ctccagcagg gctggcaatt tagacaatga gcgctgacgg
     1321 acgtgttcgc acaaatcgcg caggctttgc agctcttctt cgtcggtcaa aacggctttg
     1381 cgtttggtca tcagcatatc catcgcggta cgcaggcttt tgcgcaaagg cttgtcttga
     1441 agggaaattg cggcgttttg cttgaaagtt tccggcttca tgtgaaattt gatggtttgc
     1501 gtagtcatgc gttttcctcc aaatcggcag gggaaatgtg gtcgggcagg atggcgagga
     1561 tgaccaaatc gcgcgggccg tgcgcgccgt aagcaagcgt cagttggatg tctgcggttt
     1621 tggacgggcc ggaaatcagg aatacattgg tcggcatacc gttttccacc agtttttcgc
     1681 cttcgacggc gttgtgaaac tcgttgtaca tcttggacgt atcgaacagg cagaaatgca
     1741 cgggcggaac gagactcaaa gtacgcggct cttcggggct ggaaaacagc atcagcgtgc
     1801 cggtacgggc gatgccgcat tgcgagctgc taaagcctgc atcgatgttc gcgaagaact
     1861 cagttttcca agtatcgatt tcgcgctcga aagcaatcgg ttcgatattg ctgcccgcca
     1921 atgcggcacg cgccagttga ccgtgctcgg ttgcgagagg cagcaggatg tttttcaaac
     1981 ccttgccttc tgccgcttcg cggaaaacct gcatccaatt gcttttcgtc acccaataaa
     2041 tttcggtttt gaccgcgcgc atagcggcag cccaatgttt cagacgctca acttcgctgc
     2101 cccaagaaac acccatttca cggtaataat caaaaaccgc aggctcttcc atcggcaatg
     2161 cgtcggcttt tttcagtttt gccaaaatat tttcgcgcgc gctcatgctt tgcctccggt
     2221 acgttccaac aagaaggatg cgatgtgttt cggacgcggc atattcggtt cgtccttagc
     2281 aattttgccg ccgatgttca tcatacagcc gcagtccgcg ctgatgattt cggttgcgcc
     2341 ggtttctttc agcgcggcga ctttgtcggt taccatcgcg ccggaaatat cggcttgttt
     2401 gacggagaat gtgccgccga agccgcaaca ttcgctttcg tggtcgtgga cgatgcgttc
     2461 gacgttttcc ataccgtcaa tcagttgcca gcccgagaga tggacattca tttcgcggcg
     2521 ggcggcgcag gaagtgtgaa cggcgacttt gagcggttcg cccttgtctt cgggtttgaa
     2581 accgatggca agcaggaaat gggtaaactc gatgatgcgg ccggcgcaat ccacagccct
     2641 ttcctcgtac tcgctgcctt taaacagcgt cggccaatgg tgcttcatca taccgccgca
     2701 cgaaccggac ggtacgacaa tcggccactc ctcggggaaa aggtcgagtt gcgctctggc
     2761 gacatcaaag gcttcggtcg gatggccgga tgaataggcg ggctgaccgc agcagctttg
     2821 cgccatcggg aaatggacgc gtatgccctg ttgctcgatt agggtaatgg catccatgcc
     2881 tgcttcgggc ataaaaagat caaggacgca agtgccgaaa aaatagacat cggtcggatt
     2941 gctgtcgtat cggatgattt ttgggggaat ggttgcgctc atgttttttg ataacgggaa
     3001 acccgttttt ttctgtagaa aggtaagcgt ttactttaag taattgactg ttgcgggtca
     3061 agtctaattt taaaaaataa tccggttttt cttacaaact gccccataac gcttactgta
     3121 ccttaatctg atggttttcg ataataatta tcattacaat gcaataccgg ttcgtttgct
     3181 tgtgaacatt caagatgccg actctgacgg cattcagaca gcatctgaaa acaataacgg
     3241 cacaaggatg gggtacaatc gctctatgga aaaaaacacc ctatccgccc gcgcaccatc
     3301 cccttggctg ccgcttctgc tggcaattgc catttttatg cagatgttgg atgcgaccat
     3361 tttaaatacc gcactgcctg aaattgccgc cgacctgaat gagtcgccat tgaacatgca
     3421 gctggcggtt atttcctaca cgctgacggt tgccctgctg attcctttga gcggttattt
     3481 ggcggacagg ttcggaacga aaaaagtctt tttcggttcg attgccgttt ttatgctcgg
     3541 atcggcattg tgcgccgcat cgggttcgct gtttgaattg acgctttccc gtgtcgttca
     3601 gggcatcggc ggttcgatgc tggttccgat accgcgtctg accatcttgc gtgtgtacga
     3661 caagtccaag ctgctcaacg ccatcaatta tgcggttatg cccgcattaa tcggaccggt
     3721 tttagggcct ttggcgggcg gttatctggt cgaatacgct tcgtggcact ggattttcct
     3781 gctcaacctg cccatcggtc ttttgggttt catattggga cgcaacatca tgcccgatgt
     3841 caaaggcaac gataccgctc tggatttcaa aggctatctg attttttctg ccgccgcgtg
     3901 cctcttgtta ctttcggcag aaagcctgtc gcacgcgctg cctccgtatt ttgcactgtt
     3961 gccgctgtgc ggcggactgc tgtttgcacg ccgttatttc cgacatatga aaaccgcgtc
     4021 caaaccgatt tattccgccg acctgtttct gatacgcact ttccgtctgg gactggcggg
     4081 caatctgttc agccgtctcg gcatcagctc gattcctttt ctgatgcccc tgatgtttca
     4141 agtcgctttc ggcttcggcg caagcctgtc gggttggctg gtcgcccccg tcgccctgtc
     4201 ttcgctgctg gtcaaaccgc tgattgcacc gctcatgaaa cgtttcggct accgcacggt
     4261 actgctttgg aacaccaagc tgcttgccgc cttcatcatg ctgctcgccc tgcctgacgg
     4321 aaactcgccg ctgtggattt ggattttcct ctcgctggcg atcggcgcgt gcaactccct
     4381 gcagttttct gccatgaaca cactgaccct cgccgatttg cgcccgcaac agaccggcag
     4441 cggcaacagc ctgatggcgg tcaaccaaca gcttgccatc agcatgggca ttgttgtcgg
     4501 cgcattaatc cttaaaaact ggacatttct gataccggct tcttcaggtc tgcattccgc
     4561 cttccgtatg accctgctca gtatcggcgg cattaccctt gcctcgtcgt tggttttcaa
     4621 gcgtctgcac gtttcagacg gcgcaaacct gacacggaac acactgtcct aaagcggtcc
     4681 gcacgcaaaa cttttacccg tttcaacgtt tggattatga ttccgcactt ccatgcgcgc
     4741 caaccccaaa acacaggcaa tgccgtctga aaccatatcc ctgatgaaaa cacgcagcct
     4801 aatttccctt ttatgcctcc ttctctgttc atgttcttca tggttgcccc cactggaaga
     4861 acggacggaa agccgtcatt tcaatacttc caaacccgtc cgcctggaca acatcctgca
     4921 aatccggcac acccctcata ccaacgggct atccgatatc tatctgttga acgaccccca
     4981 cgaagccttt gccgcccgcg ccgcccttat cgaatctgcc gaacacagcc tcgatttgca
     5041 atactacatc tggcgcaacg acatttccgg ccgactgctg ttcaacctga tgtaccttgc
     5101 cgcagaacgc ggtgtgcgcg tacgcctgct gttggacgac aacaacacgc gcggattgga
     5161 cgacctcctg ctcgccctcg acagccatcc caatatcgaa gtgcgcctgt tcaacccctt
     5221 cgtcttccgc aaatggcgcg cactcggcta cctgactgac ttcccccgcc tcaaccgccg
     5281 catgcacaac aaatccttta ccgccgacaa ccgcgccacc atactcggcg gacgcaatat
     5341 cggcgacgaa tacttcaaag tcggtgagga caccgttttc gccgacctgg acatcctcgc
     5401 caccggcagc gtcgtcggcg aagtatcgca cgacttcgac cgctactggg caagccattc
     5461 cgcccacaac gccacgcgca tcatccgcag cggcaacatc ggcaagggtc ttcaagcact
     5521 cggatacaac gacgaaacgt ccagacacgc gctcctgcgc taccgcgaaa ccgtcgaaca
     5581 gtcgcccctc taccaaaaaa tacaggcagg acgcatcgac tggcagagcg tccaaacccg
     5641 cctcatcagc gacgaccctg caaaaggact cgaccgcgac cgccgcaaac cgccgattgc
     5701 cgggcggctg caagacgcgc tcaaacagcc cgaaaaaagc gtctatctgg tttcacccta
     5761 tttcgtcccc acaaaatccg gcacagacgc actggcaaaa ctggtgcagg acggcataga
     5821 cgttaccgtt ctgaccaact cgctacaggc gaccgacgtt gccgccgtcc attccggcta
     5881 tgtcaaatac cgaaaaccgc tgctcaaagc cggcatcaaa ctctacgagc tgcaacccaa
     5941 ccatgccgtc cccgccacaa aagacaaagg cctgaccggc agctccgtaa ccagcctgca
     6001 cgccaaaacc ttcattgtgg acggcaaacg catcttcatc ggttcgttca acctcgaccc
     6061 ccgttccgcg cgtctcaaca ccgaaatggg cgttgttatc gaaagcccca aaatcgcaga
     6121 acagatggag cgcacccttg ccgataccac acccgaatac gcctaccgcg ttaccctcga
     6181 caggcacaac cgcctgcaat ggcacgatcc cgccacccga aaaacctacc cgaacgaacc
     6241 cgaagccaaa ctttggaaac gcatcgccgc aaaaatccta tccctgctgc ccatagaaag
     6301 tttattatag aaatatagcg gattaacaaa aaccagtacg
//
LOCUS       DAWHSK010000110         6290 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.110, whole genome shotgun sequence.
ACCESSION   DAWHSK010000110 DAWHSK010000000
VERSION     DAWHSK010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6290
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.110"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..181)
                     /locus_tag="WIC03_001302"
     CDS             complement(<1..181)
                     /locus_tag="WIC03_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
                     /protein_id="HEZ3883797.1"
                     /translation="MLKTLTFAALHFSTAFSVTYVLTGSIGISGAVALVEPLINTVVF
                     YSGLTKTSTALPRLSS"
     gene            303..1256
                     /locus_tag="WIC03_001303"
     CDS             303..1256
                     /locus_tag="WIC03_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEZ3883798.1"
                     /translation="MNHKKIVVLDADTLPGRVFHFDFPHELAVYGTTGADETAERVRD
                     AHIVITNKVMISADIIAANPQLELIAVSATGVNNVDIGAAKAAGVAVCNVRAYGNESV
                     AEHAFMLMIALMRNLPAYQRDVAAGLWEKSPFFCHYGAPIRDLNGKTLAVFGRGNIGR
                     TLAGYAQAFGMGVVFAEHKHAPDVREGYVSFEDAVQTADVLSLHCPLNAQTENMIGEN
                     ELRQMKPGAVLINCGRGGLVDENALLAALKYGQIGGAGVDVLTEEPPRGGNPLLNARL
                     PNLIVTPHTAWASREALDRLFDILLANIHAFVKGEAQNRVV"
     gene            complement(1332..3389)
                     /gene="metG"
                     /locus_tag="WIC03_001304"
     CDS             complement(1332..3389)
                     /gene="metG"
                     /locus_tag="WIC03_001304"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEZ3883799.1"
                     /translation="MTRKILVTSALPYANGSIHLGHMVEHIQTDVWVRFQKLRGHECH
                     YCCADDTHGTPVMLAAQKQGIAPEDMIAKVREEHLADFTGFNIGYDNYYSTHSPENKQ
                     FSQDIYRALKANGKIESRVIEQLFDPEKQMFLPDRFVKGECPKCHAHDQYGDNCEVCG
                     TTYSPTELINPYSAVSGAKPELRESEHFFFKLGECADFLKAWTSGNNPHDGKPHLQAE
                     ALNKMKEWLGEGEETTLSDWDISRDAPYFGFEIPDAPGKYFYVWLDAPVGYMASFKNL
                     CDRIGIDFDEYFKADSQTEMYHFIGKDILYFHALFWPAMLHFSGHRAPTGVYAHGFLT
                     VDGQKMSKSRGTFITAKSYLEQGLNPEWMRYYIAAKLNSKIEDIDLNLQDFISRVNSD
                     LVGKYVNIAARASGFIAKRFEGRLKDVADSALLAKLAAESDTIAEQYENREYARALRD
                     IMALADAVNEYVDANKPWELAKQEGQDERLHEVCSELINAFTMLTAYLAPVLPQTAAN
                     AAKFLNLEAITWANTRETLGEHAINKYEHLMQRVEQKQVDDLIEANKQSIAAAAAPAA
                     EESKYEKVAEQASFDDFMKIDMRVAKVLNCEAVEGSTKLLKFDLDFGFEKRIIFSGIA
                     ASYPNPAELNGRMVIAVANFAPRKMAKFGVSEGMILSAATADGKLKLLDVDTGAQPGD
                     KVG"
     gene            complement(3505..5343)
                     /gene="glmS"
                     /locus_tag="WIC03_001305"
     CDS             complement(3505..5343)
                     /gene="glmS"
                     /locus_tag="WIC03_001305"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEZ3883800.1"
                     /translation="MCGIVGAIRAHHNVVDFLTDGLKRLEYRGYDSSGIAVNTDGKIK
                     RVRRVGRVQLMEDAAREKGISGGIGIGHTRWATHGGVTEPNAHPHISGGMIAVVHNGI
                     IENFESERKRLEGLGYRFESQTDTEVIAHSINHEYAQNGGRLFEAVQEAVKRFHGAYA
                     IAVIAQDKPDELVVARMGCPLLVALGDDETFIASDVSAVIAFTRRVAYLEDGDIALLA
                     SDGIKRLTDKNGLPAERKVKVSELSLASLELGPYSHFMQKEIHEQPRAIADTAEVFLD
                     GGFIPENFGKNAKSVFESIRSVKILACGTSYYAALTAKYWLESIAKIPADVEIASEYR
                     YRSVIADPDQLVITISQSGETLDTMEALKYAKSLGHRHSLSICNVMESALPRESSLVL
                     YTRAGAEIGVASTKAFTTQLVALFGLAVTLAKVRGLVSEEDEARYTEELRQLPGSVQH
                     ALNLEPQIAAWAQQFAKKTSALFLGRGIHYPIALEGALKLKEITYIHAEAYPAGELKH
                     GPLALVDENMPVVVIAPNDSLLDKVKANMQEVGARGGELFVFADLDSNFNATEGVHVI
                     RAPRHVGELSPVVHTIPVQLLAYHTALARGTDVDKPRNLAKSVTVE"
     gene            5621..6139
                     /locus_tag="WIC03_001306"
     CDS             5621..6139
                     /locus_tag="WIC03_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883801.1"
                     /translation="MKQMLLAVGVAAVLAGCGKDAGGYEGYWREKSDKKEGMIAVKKE
                     KGNYFLNKINVVTGKEESMLLSEKDGELSINTGIGEIPIKLSDDGKELYVERRQYVKT
                     DAAMKDKIIAHQKKCGQTAQAYRDARNALPSNQTYQQRQAAIEQLKRRFEAEFDELEK
                     EIKCNGKPTLLF"
BASE COUNT         1351 a         1488 c         1901 g         1550 t
ORIGIN      
        1 ttgagctaag gcgaggcaac gccgtactgg tttttgttaa tccactatag aaaacgacgg
       61 tgttgattag gggttcgacc agcgcgaccg ccccggatat gcctatactg cccgtcagta
      121 cataggttac actgaaggcg gtgctgaaat gcagtgcggc aaaagtcagg gttttaagca
      181 tcatcctctc ccgaattgga cattgacgga ggaatgataa aggttatcat aaggttgcgc
      241 ggtttaaatt tgctatttat tgttagcgta aataaatcat tttccaaata aggatagaaa
      301 ttatgaatca taaaaagatc gttgttttgg atgcggatac tttgcccggt cgggtctttc
      361 attttgattt tccgcacgag cttgcggttt acggtacgac aggtgcggat gaaacggcgg
      421 aacgggtgcg cgatgcacat attgtcatta ccaataaggt aatgatttcc gccgacatta
      481 ttgcggctaa tccgcagttg gagctgattg ccgtcagtgc gaccggtgtg aacaatgtcg
      541 atattggggc ggcgaaggcg gcgggcgttg cggtatgcaa tgtccgcgca tacggaaacg
      601 aatcggttgc ggaacacgcc tttatgctga tgattgcgtt aatgcggaat ttgcctgcct
      661 atcagcgtga tgttgcggca ggattatggg aaaaatcgcc gtttttctgc cattacggcg
      721 cgccgattcg ggatttgaac ggcaaaacgc tggcggtttt cggacgcggc aatatcggac
      781 ggacgcttgc cggatacgcg caggcattcg gtatgggggt ggtgtttgcc gaacacaaac
      841 acgcgcccga cgtgcgcgaa ggctatgttt cctttgaaga tgcggtacag actgccgacg
      901 tgttgtcgct gcactgtccg ctgaacgccc aaactgaaaa tatgataggc gaaaacgaat
      961 tgcggcagat gaagcccggt gcggttttga ttaattgcgg gcgcggcggg ctggtggatg
     1021 aaaacgcgct gcttgccgca ctcaaatacg ggcagatcgg cggagctggt gtcgatgttt
     1081 tgacggagga gccgccaagg ggcggcaatc ccttgctgaa tgcacgactt cccaatctga
     1141 ttgttacgcc gcataccgcg tgggcgagcc gcgaggcttt ggacaggctg tttgatatat
     1201 tgttggcgaa cattcacgcc tttgtgaaag gtgaggcgca aaatcgggtg gtttgaacct
     1261 gtcgggattg cggaaaaaat gccgtctgaa cgcccaaagg gttcagacgg cattgttttt
     1321 gatatttcta cttaaccgac tttgtcgccc ggttgcgcgc ctgtatccac atccaagagc
     1381 ttcagtttcc cgtctgccgt ggcggcggag agaatcatgc cttcagatac gccgaatttt
     1441 gccattttgc gcggggcgaa gttggcgacg gcaatgacca tgcggccgtt caattcggca
     1501 gggttcgggt aagacgcggc gatgccggag aagataatgc gtttttcaaa accaaaatcg
     1561 aggtcgaatt tcaaaagttt ggtgctaccc tcgacggctt cgcagttcaa tactttggcg
     1621 acgcgcatgt cgattttcat gaagtcgtcg aagctcgctt gttcggcgac tttttcgtat
     1681 ttgctctctt cggcggcagg cgcggctgct gcggcgatgc tttgtttgtt ggcttcgatt
     1741 aaatcgtcca cttgtttttg ctccactcgt tgcattaaat gttcgtattt gttgatggcg
     1801 tgttcgccca aagtttcgcg tgtatttgcc caagtgatgg cttccaaatt caggaatttg
     1861 gcggcgtttg cggcggtttg cggcaagacg ggggcgaggt aggcggtcaa catggtgaag
     1921 gcgttgatga gctcgctgca cacttcgtgc aggcgttcgt cttgaccttc ctgtttcgcc
     1981 agttcccaag gtttgttggc atcaacgtat tcgttcaccg catcagccaa agccataatg
     2041 tcgcgcaggg cgcgggcgta ttcgcggttt tcgtattgct cggcgatggt gtcgctttcg
     2101 gctgcaagtt ttgccagcaa tgcgctgtcg gcaacgtctt tcagacggcc ttcaaagcgt
     2161 ttggcgatga aacctgaagc gcgggcggcg atgttgacgt atttgccgac gaggtcgctg
     2221 tttacgcgcg aaataaagtc ttgcaggttc aaatcgatgt cttcgatttt gctgttgagt
     2281 tttgcggcga tgtagtagcg catccactcg gggtttaaac cttgttccag ataggatttg
     2341 gcggtgataa acgtgccgcg cgatttggac attttttgtc cgtctacggt caaaaagccg
     2401 tgtgcgtaca cgccggtcgg ggcgcggtgg ccggagaaat gcagcatggc aggccagaac
     2461 agggcgtgga aatagaggat gtctttgccg atgaagtggt acatctcggt ttggctgtcg
     2521 gctttgaagt attcgtcgaa atcgatgccg atgcggtcgc acaggttttt aaacgacgcc
     2581 atgtagccga cgggcgcgtc cagccagacg tagaagtatt tgcccggcgc gtcggggatt
     2641 tcaaaaccga aatacggcgc gtcgcgggaa atatcccagt cggacagggt ggtttcttca
     2701 ccttcgccca gccattcttt cattttgttg agggcttcgg cttgcagatg gggcttgccg
     2761 tcgtgcgggt tgttgccgga agtccatgct ttgaggaagt cggcgcattc gcccagtttg
     2821 aagaagaagt gttcggattc gcgcaattcg ggtttcgcgc cggaaacggc ggaatacggg
     2881 ttgatcagtt cggtcgggga ataggtcgtg ccgcagactt cgcagttgtc gccgtattgg
     2941 tcgtgggcgt ggcatttggg gcattcacct ttgacgaaac ggtcgggcag gaacatttgt
     3001 ttttcagggt cgaaaagctg ctcgatgacg cggctctcaa tcttgccgtt ggctttcagc
     3061 gcgcggtaaa tgtcttggga aaactgtttg ttttcagggg aatgggtgct gtaataattg
     3121 tcgtagccga tgttgaaacc ggtaaagtcg gcaaggtgct cttcgcgcac tttggcaatc
     3181 atgtcttcag gcgcaatgcc ttgtttttgc gcggcaagca tcacgggcgt gccgtgggtg
     3241 tcgtcggcgc agcagtagtg gcactcgtgg ccgcgcagtt tttggaagcg tacccaaacg
     3301 tcggtttgga tgtgttcgac catgtggccg aggtggatgc tgccgttggc atagggcagg
     3361 gcggaggtaa ctaagatttt gcgtgtcata ttgtgctttg caaacaatgg gtaaaggcgg
     3421 attataccgc aaatcaaacg gggaaatgcc gtctgaagcc tgaaaaatcg ggcttcagac
     3481 ggcatttttg caaaacggcg ggagttattc gaccgtaacg gatttcgcca ggttgcgcgg
     3541 tttgtctaca tcggtgccgc gtgcgagggc ggtgtggtag gcgaggagct gcacggggat
     3601 agtatgcacg acgggggaca gttcgccgac gtggcgcggt gcgcggataa cgtgcacacc
     3661 ttcggtggca ttaaaattac tgtcgaggtc ggcaaagacg aaaagttcgc cgccgcgcgc
     3721 gccgacttcc tgcatattgg ctttgacttt gtccaacagg ctgtcgttgg gtgcgatgac
     3781 gacgacgggc atgttttcgt ccaccaaagc aagcggtccg tgcttcagtt cgccggctgg
     3841 ataggcttcg gcgtggatgt aggtgatttc cttcagcttc aacgcacctt cgagggcaat
     3901 cgggtaatgg atgccgcgcc ctaaaaacag cgcgctggtt ttcttggcaa actgttgcgc
     3961 ccatgcggca atttgcggtt cgaggttcaa agcgtgctgc acgctgccgg gaagctggcg
     4021 gagttcttcg gtgtaacgcg cttcgtcttc ttcggaaacc aaaccgcgca ctttcgccag
     4081 cgttaccgcc aaaccgaaca gcgcgaccag ctgcgtggta aacgccttgg ttgaggcgac
     4141 gccgatttcc gcaccggcac gggtataaag cacgaggctg ctttcgcgcg gcagggcgga
     4201 ttccatcacg ttgcagatgg agaggctgtg gcggtgtccc aacgatttgg cgtatttcaa
     4261 agcctccatc gtgtccagcg tttcgccgga ttgggaaatg gtaatgacca gttggtccgg
     4321 atcggcaatc acgctgcggt atcggtattc gctggcgatt tcgacatcgg ccgggatttt
     4381 ggcgatggat tccaaccaat atttggcggt cagcgcggcg taataggacg tgccgcaggc
     4441 aaggattttg acgctgcgga tgctttcaaa cacgcttttg gcgtttttgc cgaagttttc
     4501 ggggatgaag ccgccgtcga ggaaaacctc cgccgtgtct gcaatcgcgc gcggctgctc
     4561 atggatttcc ttttgcataa agtggctgta cggtcccagt tccaaagagg cgagcgagag
     4621 ttcggatacc ttgactttgc gttcggcagg caggccgttt ttatcggtca gccttttgat
     4681 gccgtctgaa gccagcagcg cgatgtcgcc gtcttcgagg tacgccacgc ggcgcgtaaa
     4741 ggcgatgacg gcggatacgt ccgaagcgat aaaggtttca tcgtcgccca aagcgaccaa
     4801 aagcgggcag cccatacgcg ccacaactaa ttcatcaggc ttgtcttggg caataaccgc
     4861 gatggcgtat gcgccgtgga aacgtttgac cgcttcttgt accgcttcaa acagcctgcc
     4921 gccgttttgc gcgtattcgt gattgatgct gtgtgcgatg acttcggtat ccgtttgcga
     4981 ttcaaaacgg tatcccaaac cttccaaacg tttgcgttcg ctttcaaagt tttcgatgat
     5041 gccgttgtgt acgaccgcaa tcataccgcc gctgatgtgc gggtgggcgt tcggctcagt
     5101 aacgccgccg tgtgtcgccc aacgcgtatg tccgatgccg atgccgccgc tgatgccttt
     5161 ttcgcgtgcc gcgtcctcca taagctgcac gcgtccgacg cggcgcacac gtttgatttt
     5221 gccgtcggtg ttgacggcaa tgcctgatga gtcataaccc cggtattcga ggcgtttgag
     5281 accgtcggtc agaaaatcga cgacgttgtg atgggcgcgg atggcgccga cgataccgca
     5341 cataactgtt ccttagtatc cggttgaaaa aaaacaggcg cggacggctt ccgtgccgca
     5401 ccttcctctt cggattataa accgcctccc gcgccggaaa acagcaaaat gccgtctgaa
     5461 ggcttgggct tgctcaaaaa aaggagggat ttccccgcct aggatgggcg ttcagacggc
     5521 attgcctgct gctggtttat agtttttaca aatcaatatt gacaagctga aaaaaaaaaa
     5581 caatatactc acccgatctt aatgttaaag gagtatggaa atgaaacaaa tgcttttggc
     5641 cgtcggcgtg gcggcggtgt tggcaggctg cggcaaggat gccggcggtt acgagggtta
     5701 ttggcgcgaa aagtcggaca aaaaagaggg tatgattgcc gtcaaaaaag aaaaaggcaa
     5761 ttacttcctt aataaaatca acgtggttac aggtaaagaa gaatctatgc ttttgtctga
     5821 aaaagacggc gagctttcga taaacacggg gataggggaa atcccgatca aactttccga
     5881 cgacgggaaa gagctgtatg tcgaacgcag gcagtatgtc aaaaccgatg cggcgatgaa
     5941 ggacaaaatc atcgcccatc agaaaaagtg cggacaaaca gcacaggcat accgcgacgc
     6001 gcgaaatgcg ttgccgtcaa accaaacgta tcagcagcgt caggcggcga tcgagcaatt
     6061 gaaacggcgg tttgaagccg agtttgacga attggaaaaa gaaatcaaat gcaacggcaa
     6121 accgacattg ttgttttagc agaggacaac cgggaggatg ccgccgtccg aatcggacgt
     6181 gcggtttccg taccggtatg ggcgggcggg aatgtccgcc ttttttgttc ggacgcgttt
     6241 gaatacccgt ccgattccgt ccgtttgcaa agggtatttt tgttcgggcg
//
LOCUS       DAWHSK010000111         6275 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.111, whole genome shotgun sequence.
ACCESSION   DAWHSK010000111 DAWHSK010000000
VERSION     DAWHSK010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6275
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.111"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            142..1539
                     /gene="aspA"
                     /locus_tag="WIC03_001307"
     CDS             142..1539
                     /gene="aspA"
                     /locus_tag="WIC03_001307"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HEZ3883802.1"
                     /translation="MTVRIEHDLLGDREIPAEVYWGIHTLRAIENFKISTQKISDVPQ
                     FVRSMVMVKKATAQANGELGAVKPEIAAAIEKACDEVLLNNRCLDQFPSDVYQGGAGT
                     SVNMNTNEVIANLALEVLGYEKGRYDIVNPMDHVNASQSTNDAYPTGFRLAVYYSIGE
                     LLDKLTVLKNAFAAKAEEFKDVLKMGRTQLQDAVPMTAGQEFQSFQVLLEEEILNLDR
                     TRQLLLEVNLGATAIGTGVNTPKGYAELVVKKLSEVSGLPCKLTENLIEATSDCGAYV
                     MVHGALKRTAVKLSKICNDLRLLSSGPRAGLKEINLPELQAGSSIMPAKVNPVIPEVV
                     NQVCFKVIGNDTTITFAAEAGQLQLNVMEPVIAQCMFETISLLGNAAVNLSDKCVKGI
                     TVNREICERYVFNSIGLVTYLNPYIGHHNGDLVGKICAQTGKGVREVVLERGLLSEEE
                     LNRILSPENLMNPHL"
     gene            complement(1611..2486)
                     /locus_tag="WIC03_001308"
     CDS             complement(1611..2486)
                     /locus_tag="WIC03_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ3883803.1"
                     /translation="MAYLLISIVFSVSVSILLKMARKKKIDIAQAVAVNYVVAVILTL
                     LVLKPDIGNIGAFLPTWPLFAALGVLLPSVFVIMGKSVEAAGIVKSDAAQRLSLFLPI
                     VAALTLFGEKLSEGKLIGLCLAFAALFCLLWKHSGGKKSGSAWRQAALLLGVWAGYGI
                     IDILFKQLAKSGTAFAGNLLVAFVLAGVLMFACLFAKSVRWRVESVVGGIFLGGLNFM
                     NIVTYITAHQMMKDNPTLVFAGMNIGVIVLGTLSGALFFKEKINTINTAGIVLALCSI
                     ACLFYWGEVKVLFGI"
     gene            complement(2488..3210)
                     /locus_tag="WIC03_001309"
     CDS             complement(2488..3210)
                     /locus_tag="WIC03_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEZ3883804.1"
                     /translation="MDKDLYAVLGVSPQAGADEIKRAYRKLAMKYHPDRNPGNPKAEE
                     KFKEIQRAYDTLSDLSKRMQYDASFRRHEERGRQEEAFRREQARREQFYREQMRREQA
                     FRQAFERQASRSCHTYEPSGGGSGRNYVLAAYILFGLGAIMLFMPIVGVIFAYMPIVG
                     VILAYMKRNSLDSIVYAAHTEYLIKTFWRTFWLYILGALTALLGIGVLIIIATNVWYF
                     YRIIAGFIRFNGGRAVAPEKWI"
     gene            complement(3233..3712)
                     /gene="ybaK"
                     /locus_tag="WIC03_001310"
     CDS             complement(3233..3712)
                     /gene="ybaK"
                     /locus_tag="WIC03_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HEZ3883805.1"
                     /translation="MSKHTYPITPAVRVLRENGIEFEPFTYAYEEHGGTAQFARLFGK
                     DEHLVIKTIVLQDENGKGLIVLMHGDKQISTRNLARHLGAKHIEPATPVQANKWTGYL
                     VGGTTPFGIRTKLDIYVEQSVMDLETIYINGGKRGFIIGIRPGDLNILNPKTIQAAV"
     gene            3787..4149
                     /locus_tag="WIC03_001311"
     CDS             3787..4149
                     /locus_tag="WIC03_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEZ3883806.1"
                     /translation="MDIRYFGTTPRYSEAVGANGLIFLSGMVPENGETAAEQTADVLA
                     QIDRWLAECGSDKAHVLDAVIYLRDMGDYAEMNGVWDAWVAAGRTPARACVEARLARP
                     EWRVEIKITAVKKDAATA"
     gene            4184..5047
                     /locus_tag="WIC03_001312"
     CDS             4184..5047
                     /locus_tag="WIC03_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein YgfZ"
                     /protein_id="HEZ3883807.1"
                     /translation="MKTLLPFFGVVRVSGEDRQTFLHGQLSNDINNLQTGQACYATYN
                     TPKGRVIANMIVVNRGGDLLLIMAQDLLEATVKRLRMFVLRAKAVFEILEDYAVGAEL
                     EASAEPLAAQEPNLAFAAQQDSDGICSIALPHGGILRIAPKNTLPPYDAAAENAWRLH
                     EIRSGYPWICAATKETAVAQMLNQHIIGGVHFKKGCYPGQEIIARAQYRGQVKRGLAV
                     LSGNSAVEAGTLLTADGEETGIVLDSVQDSENFTALAVIKFSAAQKELTAPNGSIFKA
                     VHPFFKTENAE"
     gene            5145..6104
                     /gene="ttcA"
                     /locus_tag="WIC03_001313"
     CDS             5145..6104
                     /gene="ttcA"
                     /locus_tag="WIC03_001313"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HEZ3883808.1"
                     /translation="MSKKTKQELENNKLSKRLRHAVGDAINDFNMIEPGDKIMVCLSG
                     GKDSYALLDILRQLQASAPIDFQLVAVNLDQKQPGFPEEVLPTYLESIGVPYKIVEED
                     TYSTVKRVLDEGKTTCSLCSRLRRGILYRTAKELGCTKIALGHHRDDILATLFLNMFY
                     GGKLKAMPPKLVSDNGEHIVIRPLAYVKEKDLIKYAELKQFPIIPCNLCGSQPNLQRQ
                     VIGDMLRDWDKRFPGRIESMFSALQNVVPSHLADTELFDFAGLERGQTLKHGGDLAFD
                     SEKMPERFSDGSEEDESEIKIEPQKAERKVINILANKPKTCGA"
BASE COUNT         1589 a         1819 c         1504 g         1363 t
ORIGIN      
        1 cccgaaaaca acgtttcccg ttttcagacg gcatacgata tggacaattt catccggccg
       61 gtatagtgtt catcctgcgg cggcatcttt ccgatggcgc attttttcaa tattaattat
      121 ccgacttaca aaggtaaaaa catgactgtc cgtatcgaac acgatttatt gggcgaccgc
      181 gagattcccg ccgaagtgta ttggggcatc cacaccctgc gcgccattga aaactttaaa
      241 atctccacac aaaaaatttc tgacgtgccg cagtttgtcc gcagtatggt gatggtgaaa
      301 aaggcgacgg cacaggcaaa cggtgagttg ggcgcggtca agcccgaaat cgccgccgcc
      361 attgaaaaag cctgcgacga agttctgctg aacaaccgct gcctcgacca attcccgtcc
      421 gacgtgtatc agggcggtgc gggtacttcg gtcaatatga acaccaacga agtcatcgcc
      481 aacctcgcat tggaagtctt gggatacgaa aaaggccgct acgacatcgt caatccgatg
      541 gatcacgtca atgccagcca atcgaccaac gatgcctatc ccacgggctt ccgccttgcc
      601 gtgtattaca gcatcggcga attgctcgac aaactgactg tcctgaaaaa cgcctttgcc
      661 gccaaagccg aagaatttaa agacgttttg aaaatgggcc gcacccagct tcaagatgcc
      721 gtgccgatga ctgccggcca agaattccaa tctttccaag tgttgttgga agaagaaatc
      781 ctcaacctcg accgcacccg ccaactgctg ttggaagtca atttgggcgc aacggcaatc
      841 ggcacgggcg tgaacacgcc caaaggctac gccgaactgg tggtcaaaaa actctccgaa
      901 gtcagcggct tgccttgcaa actgaccgaa aacctgattg aggcgacctc cgactgcggc
      961 gcatatgtga tggtacacgg cgcattgaag cgcacggcgg tcaaactctc caaaatctgc
     1021 aacgacttgc gcctgctctc ttccggcccg cgtgccggct tgaaagaaat caacctgccc
     1081 gaattgcagg ccggttcttc cataatgccc gccaaagtca atcccgtgat tcccgaagtg
     1141 gtcaaccaag tctgcttcaa agtcatcggc aacgacacga cgattacctt cgccgccgaa
     1201 gccgggcaac tgcaattaaa cgtgatggag ccggtcatcg cccaatgtat gtttgaaacc
     1261 atttccctct tgggcaatgc cgcagtcaac ctgtccgaca aatgcgtcaa aggcattacg
     1321 gtcaaccgcg aaatctgcga acgttacgtt ttcaactcca tcggtttggt gacttatctg
     1381 aatccgtata tcggacacca caacggcgat ttggtcggca aaatctgcgc ccaaaccggc
     1441 aaaggcgtgc gcgaggttgt cttggaacgc gggctgttga gcgaagaaga actcaaccgc
     1501 atcctctctc ccgagaacct gatgaacccc catctgtaac gttgcagaca aatgccgtct
     1561 gaagcaggga tgctgcttca gacggcattt tgttatatca atcagccgga ttatatgccg
     1621 aacagtacct tgacttcccc ccaataaaac aggcaggcga tagaacacag tgccaacacg
     1681 attcccgccg tattgattgt gttgattttt tccttaaaga acaatgcgcc cgaaagcgta
     1741 cccaaaacaa tcacgccgat attcataccg gcaaaaacca aggtcggatt atccttcatc
     1801 atttggtgcg cggtgatgta ggttacgata ttcataaaat tcaaaccgcc caagaatatg
     1861 ccgccgacca cactctcaac acgccatctg accgatttgg caaacaggca ggcaaacatc
     1921 agcacacccg ccagcacaaa tgcaaccagc aggttgcccg caaatgccgt tccgcttttg
     1981 gcaagctgtt tgaacaggat atcgataatg ccgtaacctg cccacacgcc cagcagcaat
     2041 gccgcctgcc gccacgcgct tcctgatttt ttgccaccgc tgtgtttcca aagcaggcag
     2101 aacagtgcgg caaatgcgag gcacagcccg attagtttgc cttcgctgag tttttcgcca
     2161 aacagcgtca aggcggcaac aatcggcaaa aacagcgaca aacgctgcgc cgcgtcggat
     2221 ttgacgatac cggcggcttc cacagatttg cccattatca cgaatacgga cggcagcagc
     2281 acgcccaaag cggcaaacag cggccacgtc ggcaaaaatg cgccgatatt gccgatatcc
     2341 ggcttcaata ccagcagggt cagtatgacc gcgaccacat aattgacggc gaccgcctgc
     2401 gcgatgtcga tttttttctt ccttgccatt ttcagcaaaa tggaaaccga cacgctgaac
     2461 acgatgctga ttaacaggta agccatacta tatccatttc tcgggtgcaa ccgccctgcc
     2521 gccgttgaag cggataaagc cggcgatgat gcggtagaaa taccagacgt tcgttgcaat
     2581 aataatcagc acgccgatac ccaaaagggc agtcagcgca cccaaaatat aaagccaaaa
     2641 tgtgcgccaa aaggttttaa tcaggtattc ggtatgtgcg gcatagacaa tgctgtccaa
     2701 actgttccgt ttcatatagg cgagaatcac gccgactatg ggcatatagg cgaaaatcac
     2761 gccgactatg ggcatgaaca gcatgattgc acccaaaccg aacaggatgt aggcggcgag
     2821 gacatagttg cgcccgcttc cgccgccgga cggttcgtaa gtatggcacg aacgtgatgc
     2881 ctgccgttca aacgcctgtc tgaacgcctg ttcgcggcgc atctgttcgc ggtaaaactg
     2941 ctccctgcgc gcctgttcgc ggcggaatgc ctcttcctgc cgcccgcgtt cctcatgccg
     3001 tctgaaggac gcgtcgtatt gcatccgttt cgacaggtcg gaaagcgtat cgtaagcccg
     3061 ttggatttct ttgaactttt cttccgcctt cggattgccc ggattgcggt cgggatgata
     3121 tttcatcgcc agcttgcggt aggcgcgttt gatttcgtcc gctcccgcct gcggcgacac
     3181 gcccaatacg gcatacaaat ctttgtccat attgttcctt tatactttcc cgtcaaaccg
     3241 ccgcctgtat tgttttcggg ttcaaaatat ttaaatctcc gggacggatg ccgataatga
     3301 acccgcgttt tccgccgttg atatagatgg tttccaaatc catcaccgac tgttcgacgt
     3361 aaatatccaa ctttgtccgg atgccgaacg gcgttgtgcc gccgaccaga tagcccgtcc
     3421 acttgtttgc ctgtacgggc gtggcgggtt cgatgtgttt cgcacccaaa tgccgcgcca
     3481 gattgcgggt tgaaatctgc ttgtcgccgt gcatcaagac aatcagcccc ttaccgtttt
     3541 catcttgcaa aacaatggtt ttaatgacca agtgttcgtc tttgccgaat agtcgggcaa
     3601 actgcgccgt gccgccgtgt tcctcatagg cataggtaaa aggttcaaat tcgatgccgt
     3661 tttcacgcaa aacgcgcacg gcaggtgtta tcggataagt gtgtttactc atttttcgtc
     3721 atgcaaaaca tggaaatcgg tcaagcgcaa tgttaccata tatccccttt atcgaaggaa
     3781 accgatatgg atatccgata tttcggcaca acgccccgct attccgaagc cgtcggcgca
     3841 aacggcttga ttttcctctc cggcatggtt cccgaaaacg gtgaaacggc tgccgaacag
     3901 actgccgacg tgcttgccca aatcgaccgc tggctggcgg aatgcggctc ggacaaagcg
     3961 cacgttttgg atgcggtcat ctatctgcgc gatatgggcg actatgcgga aatgaacggc
     4021 gtgtgggacg cttgggttgc cgccggcaga acgcccgccc gcgcctgcgt ggaagcgcgg
     4081 ctcgcccgtc cggagtggcg cgtcgaaatc aaaataaccg ccgtgaagaa agatgcggca
     4141 accgcataaa cagatttcct ttatatttca gagtacgcaa atgatgaaaa ccttactgcc
     4201 ctttttcggc gtggtacgcg tcagcggcga agaccgccaa accttcctgc acggacagct
     4261 ttccaacgac atcaataatc ttcagaccgg gcaggcgtgt tatgcgactt acaacacgcc
     4321 caaaggccgt gtcatcgcca atatgattgt cgtcaaccga ggcggcgatt tgctgctgat
     4381 tatggcgcaa gacttgcttg aagcaacggt caaacgtttg cggatgtttg tgttgcgtgc
     4441 caaagccgtt tttgaaatcc ttgaagatta cgccgtcggc gcggaattgg aagcatccgc
     4501 cgaaccgctt gccgcgcaag aacccaacct tgcctttgcc gcacaacaag attcagacgg
     4561 catctgctct atcgctctgc ctcatggcgg catccttcgc atcgccccca aaaacacctt
     4621 gccgccttat gatgccgccg ccgaaaacgc gtggcggctg cacgaaatcc gtagcggtta
     4681 tccgtggata tgcgcggcta ccaaagaaac tgccgttgcc caaatgctca accagcacat
     4741 catcggcggc gtacacttca aaaaaggctg ctatcccggt caggaaatca ttgcccgtgc
     4801 ccagtatcgc ggtcaggtca aacgcggctt ggcggtattg tcgggaaatt cggccgtcga
     4861 agcaggaacc ctgctgacgg cagacggcga agagaccggc atcgtactcg acagcgttca
     4921 agattcggaa aactttaccg cccttgccgt gattaaattc tccgccgcgc aaaaagaact
     4981 gaccgcccca aacggcagta tcttcaaagc ggttcatccc ttctttaaaa ccgaaaatgc
     5041 agaataatcc ctctaatgcc gtctgaacct ttttcagacg gcattttaaa accgctcctg
     5101 ttttcggtta caatgccgca tccccaattt ttcaaaccac cgccatgtcc aaaaaaacca
     5161 aacaagaact ggaaaacaac aaactcagca aacgcctgcg ccacgccgtc ggcgacgcga
     5221 ttaacgattt caacatgatc gagccgggcg acaaaatcat ggtctgcctc tccggcggaa
     5281 aagacagcta tgccctcttg gacatcctgc gccagctcca agccagcgcg ccgattgatt
     5341 tccaactggt tgccgtcaat ctcgaccaaa agcagccggg cttccccgaa gaagtattac
     5401 cgacctatct cgaaagcatc ggcgttccct acaaaattgt tgaagaagac acctactcca
     5461 ccgtcaaacg cgtattagac gaaggcaaaa cgacttgttc gctgtgcagc cgcctgcgcc
     5521 gcggcatcct ctaccgcact gcaaaagaat tgggctgcac caaaatcgcc ttgggacacc
     5581 accgcgacga catcctcgcc accttgtttt taaatatgtt ttacggcggc aaactcaaag
     5641 ccatgccgcc caagctggtg agcgacaacg gcgaacacat cgtcatccgt ccgctggcgt
     5701 atgtaaaaga aaaagactta atcaaatacg ccgaattaaa acaattcccc atcatcccgt
     5761 gcaacctctg cggctcgcaa cccaacctgc aacgccaagt catcggcgat atgctgcgcg
     5821 attgggacaa acgctttccc ggccgcatcg aatcgatgtt ctccgccctg caaaacgtcg
     5881 tcccttccca ccttgccgat accgaactct tcgactttgc cggattggaa cgcggtcaaa
     5941 ccctgaaaca cggcggcgat ttggcgtttg acagcgaaaa aatgcccgaa cgtttttcag
     6001 acggcagcga agaagacgaa agcgaaatca aaatcgagcc gcaaaaagcc gaacgcaaag
     6061 tcatcaacat tctggcgaac aagccgaaaa cctgcggcgc ataaacctcc gccgtcaaaa
     6121 atgccgtctg aagcccgttt ggcgtttcag acggcatttt aaaatcccga agcagacggc
     6181 ttccaaacat ttgtttctca gtgtccgttt tcagaatgcc cgcctgcggg catattttgc
     6241 ccgatcagtt cggctatcct ctcgccgtca aactg
//
LOCUS       DAWHSK010000112         6259 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.112, whole genome shotgun sequence.
ACCESSION   DAWHSK010000112 DAWHSK010000000
VERSION     DAWHSK010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6259
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.112"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(392..826)
                     /locus_tag="WIC03_001314"
     CDS             complement(392..826)
                     /locus_tag="WIC03_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEZ3883809.1"
                     /translation="MAFGSMNSGDDSPMSDINVTPLVDVMLVLLIVFMITMPVLTHSI
                     PLELPTASEQTNKQDKQPKDPLRLTIDANGGYYVGGDSASKVEIGEVESRLKAAKEQN
                     ENVIVAIAADKAVEYDYVNKALEAARQAGITKIGFVTETKAQ"
     gene            complement(829..1491)
                     /gene="exbB"
                     /locus_tag="WIC03_001315"
     CDS             complement(829..1491)
                     /gene="exbB"
                     /locus_tag="WIC03_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HEZ3883810.1"
                     /translation="MNLKLVFESGDPVLIGVFVLMLLMSIVTWCLVVLRCIKLYRARK
                     GNAAVKRHMRDTLSLNDAVEKVRAVDAPLSKLAQEALQSYRNYRRNEASELAQALPLN
                     EYLVIQIRNSMAQIMRRFDYGMTALASIGATAPFIGLFGTVWGIYHALINIGQSGQMS
                     IAAVAGPIGEALVATAAGLFVAIPAVLAYNFLNRGTKILTQDLDAMAHDLHVRLLNQK
                     DS"
     gene            complement(1557..2399)
                     /locus_tag="WIC03_001316"
     CDS             complement(1557..2399)
                     /locus_tag="WIC03_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEZ3883811.1"
                     /translation="MDKERILTPAVVFSVALLHLAMVALLWQAHKLPVIESGNVIEFV
                     DLGDFGGGDGAPEGAGAPAAPEPQPVPEPPKPVEPPKPVLKPVVTKKADADIQQPKEE
                     PKPEEKPKPEEKPKPEPKPEAKPVPKPAEKPVEKPSEKPAEHPGNASAKADSEQGNGE
                     DKGTGIKGDGTGRGEGSGKGSGGVKGEHGEGAGSSKGNPLRANGSIPRPAYPTLSMEN
                     DEQGTVVLSVLVSPGGHVESVKIVKSSGFSRLDNAARKAAQNGHFQANAWTEFKVPVK
                     FELN"
     gene            2654..3292
                     /locus_tag="WIC03_001317"
     CDS             2654..3292
                     /locus_tag="WIC03_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEZ3883812.1"
                     /translation="MFQNFDLGVFLLAVLPVLLAITVREVARGYTARYWGDNTAEQYG
                     RLTLNPLPHIDLVGTIIVPLLTLMFTPFLFGWARPIPIDSRNFRNPRLAWRCVAASGP
                     LSNLAMAVLWGVVLVLTPYVGGAYQMPLAQMANYGILINAILFALNIIPILPWDGGIF
                     IDTFLSAKYSQAFRKIEPYGTWIILLLMLTGVLGAFIAPIVRLVIAFVQMFV"
     gene            complement(3744..4544)
                     /locus_tag="WIC03_001318"
     CDS             complement(3744..4544)
                     /locus_tag="WIC03_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEZ3883813.1"
                     /translation="MKKTIFNITKMDCPSEEQLIRMRLKDVSDIYELQFDIAGRCLTV
                     YHDNQDTTILQVLEPLNFDSHIISTEVIVDKIVFNKPDEHLEKRLLYQVLMINFVFFI
                     IECSVGIFANSMGLIADSLDMLADSFVYILALSAIGMTLAYKKRVAFLAGITQIILAL
                     FGVIEVIRRFIGAEQLPNYQLMIGTAFLALIANWLCLYLLSKNQNKEIHIKASMIFTS
                     NDIIINIGVIAAGALTLLTHSSYPDLIIGMIVFVIVLFGARNILKLAK"
     gene            complement(4680..5183)
                     /locus_tag="WIC03_001319"
     CDS             complement(4680..5183)
                     /locus_tag="WIC03_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883814.1"
                     /translation="MDAFDFFMQGNFKKIATEQGLDRKYFIFQYIVCNLSAISSFCLM
                     KIIHTYTILYILIAVIGIINGFLLRSKVLSIVKNKQKFLSDMFPLEMPFFFVEYKIPI
                     LARFLNRGMKWLYLPSFFCLVAICFSIYILNEEGGWMPYFACYWFGLCYPVSTYYVFL
                     SRDYKHI"
     gene            complement(5520..6161)
                     /locus_tag="WIC03_001320"
     CDS             complement(5520..6161)
                     /locus_tag="WIC03_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrxB family glutaredoxin"
                     /protein_id="HEZ3883815.1"
                     /translation="MKLYIYDHCPFCVRARMAAGLFGADVEEAVLANDDEATPIGMIG
                     AKQVPVLQKEDGSFMGESLDIVRHFDREDRLKDEVRPEIRAWLDKVGGYNDKLVQPRV
                     IKIGLPEFATPEAVKYFTDKKEKSIGSFSANLNKTAQYLERINADLQELENLMDGASD
                     GINGGIGMEDILVFPVLRNLTVVRGIAFPRKTMDYLIGMSEKSGVPLYFDRAL"
BASE COUNT         1664 a         1566 c         1307 g         1722 t
ORIGIN      
        1 gggtatagcg gattaacaaa aaccagtacg gcgttgcctc gccttaactc aaagagaacg
       61 attctctaag gtgctgaagc accaagtgaa tcggttccgt actatttgta ctgtctacgg
      121 cttcgttgcc ttgtcctgat ttttgttaat ccactataaa aattagaaat gcacattttc
      181 attattctcg cgcaggcagg actccagact tacccatttc agtaatgttt gaaaataaaa
      241 gaaaaatcag atgtttgtat tcccgcctgc gcagaaatgg agacggcgct ctgtcgtctc
      301 atttttgttt taatcaacta tatatagctg attaaacata agaaatgccg tctgaaagac
      361 tttcagacgg cattcgttca agcgtcgaac tttattgcgc cttggtttcg gttacaaaac
      421 cgattttggt gattcctgcc tgacgggcgg cttctaaagc tttgtttaca taatcgtatt
      481 ccaccgcctt gtctgccgca atcgccacaa tcacgttttc attctgctcc ttggcggctt
      541 tcagacggct ttccacttcc ccgatttcca ctttgcttgc agaatccccg ccgacataat
      601 agccgccgtt cgcatcaatc gtcaggcgca gggggtcttt aggctgtttg tcctgcttgt
      661 ttgtctgctc ggacgcggtc ggcagttcca aagggatgga atgcgtcagc accggcatag
      721 taatcataaa cacaatcagc aacaccagca tcacgtccac caacggcgta acgttgatgt
      781 cggacatcgg agaatcgtcg ccggaattca tcgaaccaaa tgccataatc agctatcctt
      841 ttgattaagc aggcggacgt gcaaatcgtg cgccatcgca tccaaatcct gggtcagtat
      901 ttttgtgccg cgattgagga agttgtatgc caacaccgcc ggaatcgcca cgaacaaacc
      961 cgccgccgtc gccaccagtg cctcgccaat cgggccggca accgccgcaa tactcatctg
     1021 cccgctttgc ccgatattga tcagggcgtg gtaaatcccc caaaccgtgc cgaacaaccc
     1081 gataaacggc gcggtcgcgc cgatggaggc aagcgcggtc atcccgtaat caaaccggcg
     1141 cataatctgc gccatactgt tgcggatttg aatgaccaaa tactcgttca acggcaaagc
     1201 ctgcgccagt tcggacgctt cgtttcggcg gtagttgcgg taagactgca atgcctcttg
     1261 cgccagtttg gacaaaggcg catcgacggc gcgcactttt tcgaccgcgt cgttcagcga
     1321 caaagtatcg cgcatatgcc gtttgacggc ggcattccct ttgcgcgccc gatacagctt
     1381 gatgcagcgc aagacaacca aacaccacgt tacgatactc atcaacagca tcaacacaaa
     1441 cacaccgatc aggacgggat cgcccgattc aaacactaat ttcaaattca taatgattcc
     1501 aacactgaaa aaaccaatca aacatccaag ctgccgcaaa ccgctgcggc aaccgcctaa
     1561 ttcaattcaa acttgacggg gactttaaac tccgtccagg cattggcttg aaaatgcccg
     1621 ttttgcgccg ccttgcgtgc cgcattgtcc aaccgggaaa aaccactgct tttcacgatt
     1681 ttaacggact caacatgacc gcccggagaa accaaaacgc tcaaaacaac cgtaccctgc
     1741 tcatcattct ccatagaaag cgtgggataa gccgggcgcg gaatgctgcc gttggcgcgt
     1801 aaaggattgc ctttgctgct gccggctcct tccccgtgtt cgcctttgac accgccgcta
     1861 cctttgccgc tgccttctcc gcgccccgtt ccgtctcctt tgataccggt tcccttatct
     1921 tccccattgc cctgctcgct gtctgctttg gcagaagcat tgccgggatg ttcggcaggt
     1981 ttttcagacg gcttctcgac cggtttttcc gccggtttcg ggacaggctt cgcttccggc
     2041 ttaggctctg gtttcggttt ttcttcgggt ttcggctttt cttcaggttt cggctcttcc
     2101 ttaggctgct gaatatccgc atccgccttt ttcgtaacca ccggcttcaa aaccggcttg
     2161 ggcggctcga caggtttggg cggctcgggc acgggttgcg gttcgggcgc ggcaggcgcg
     2221 cctgcacctt cgggtgcgcc gtcccctccg ccaaaatcgc ccaaatcgac aaattcaata
     2281 acattgcctg actctatcac gggcagcttg tgcgcctgcc agagcaatgc caccattgcc
     2341 aaatgcagca gtgcgacgga aaacacgact gcgggggtta aaattcgttc tttatccata
     2401 attcgggcat aataatagca acaattccta tttgcaacct atttttacaa tttttggtca
     2461 tatgaatgtc tgttccgttc acaggcaaac cgtgtttaaa cgctgtatta cagcaaatca
     2521 tcagataacg ggccggcaga aaaaatgatt ccgtctgatt tcttattcca ataaaatcag
     2581 gttagatgat atattgccgc ttctgtctgt cagccgtttc gggctgcaca ccacatctgt
     2641 tcaaaggaaa accatgtttc aaaattttga tttgggcgtg tttctgcttg ccgtcctgcc
     2701 cgtgctgctc gccattaccg tcagggaggt ggcgcgcggc tatacggcgc gctactgggg
     2761 agacaacact gccgaacaat acggcaggct gacactgaac cccctgcccc atatcgattt
     2821 ggtcggcaca atcatcgtac cgctgcttac tttgatgttt acgcccttcc tgttcggctg
     2881 ggcgcgtccg attcctatcg attcgcgcaa cttccgcaac ccgcgccttg cctggcgttg
     2941 cgttgccgcg tccggcccgc tgtcgaatct ggcgatggct gttctgtggg gcgtggtttt
     3001 ggtgctgact ccgtatgtcg gtggggcgta tcagatgccg ttggctcaaa tggcaaacta
     3061 cggtattctg atcaatgcga ttctgttcgc gctcaacatc atccccatcc tgccttggga
     3121 cggcggcatt ttcatcgaca ccttcctgtc ggcgaaatat tcgcaagcgt tccgcaaaat
     3181 cgaaccttat gggacgtgga ttatcctgct gcttatgctg accggggttt tgggtgcgtt
     3241 tattgcaccg attgtgcggc tggtgattgc gtttgtgcag atgttcgtct gactggcttt
     3301 cagacggcat aaacgctcca gaaaacgcgg caggacatat tgccctgccg cgttttcctg
     3361 tagtgtaatc ttattttttt catcattatt agaaccaagt tgcatgataa tacctttcat
     3421 taactgaaac actgattaag aaactccagt ctgtctaatg atgaggtttt cacatcgcca
     3481 aaacttgcca atcaaatgct ggatttattg ccgtctgaga tttggtcaaa tccaaaggcg
     3541 acattcttag accctgtgtg taaatcaggg gtatttttgc gtgaaatcgt caaacgcttg
     3601 gatgaaggct tggccaatca aataccagat aaacaaactc gcattaacca cattttaaaa
     3661 aatcaagttt ttggaagtac tgccacgtat gtaggtagct ttgaccgata tttgcataaa
     3721 aactcctttg ctggtgaaag gaattatttt gccaatttta aaatatttct ggcaccaaat
     3781 agtacaatga caaagacaat catgccaatg attaaatcag gatagctaga atgagtcaat
     3841 aacgtcaatg ctcccgccgc tatcacaccg atattgatga taatgtcatt ggatgtaaaa
     3901 atcatgctgg ctttgatatg gatttcttta ttttgatttt tgctcagtag atataagcac
     3961 agccagtttg caatcaatgc caaaaatgcc gtgccaatca tcagttgata attgggcagc
     4021 tgctcagcac cgataaaacg cctaatcact tctatcaccc caaataacgc caatattatc
     4081 tgcgttatcc ccgccaaaaa tgccacacgt tttttatacg ccagcgtcat accaatggct
     4141 gatagcgcca atatatagac aaagctgtcc gccagcatat ctagactatc agcaatcagc
     4201 cccatagaat tagcaaaaat accaaccgaa cactctatga taaaaaacac aaagttaatc
     4261 atgagcactt gatataataa tcttttttct aagtgctcat caggcttgtt aaacactatc
     4321 ttatcaacaa tcacttcggt ggaaatgata tgactatcaa aattaagcgg ttcaagtact
     4381 tgtaaaatcg ttgtatcttg attatcgtga tagacggtta agcaccgccc agcaatatca
     4441 aactgtaatt cataaatatc agacacatct tttaaacgca tgcgaatgag ctgttcttcg
     4501 gacgggcagt ccattttggt aatgttaaaa atggtctttt tcatctattt agttccttgt
     4561 tttgatcagg ttggctcaaa taaatctgtg tttatattgc tgcttggtaa tttttggatg
     4621 gtttgagtaa attgattagg ttaaaattta cctttggaag taccgccacg cataatagtt
     4681 tagatatgtt tataatctct ggataaaaaa acgtaataag tgcttactgg ataacaaagt
     4741 ccaaaccaat agcaggcaaa ataaggcatc cacccccctt cttcattaag gatatatatt
     4801 gagaaacaaa tcgcaactaa acagaaaaaa cttgggagat aaagccattt cattccccta
     4861 ttcaagaatc tagccaagat aggtattttg tattctacaa aaaagaaagg catttccaag
     4921 ggaaacatgt cagataaaaa cttttgttta ttttttacta tagatagaac cttgcttctc
     4981 aagagaaagc cattaataat accgatgaca gctattaata tatagagaat agtataagta
     5041 tgaataatct tcattagaca aaaagaagaa atggcagata aattacatac gatatattgg
     5101 aatataaaat atttacggtc taaaccttgt tcagttgcaa tttttttaaa attgccttgc
     5161 ataaaaaaat caaaggcgtc cattaaacta tctttcacat tagaaattta aaagctaaat
     5221 aatacgacaa acaatgtgaa gtactattca tggtttattt aaaaaataat actattctga
     5281 acattattta gatacagaaa ttaacaaatt agaactaaac aagcttttaa atactttaat
     5341 tttattggaa agctataaaa ggaactataa ctttacacac tagtcacttc tttttaagag
     5401 gcaaaaggga ttgggaaggt cgtcttggag ataagcactg gtatttcggc caatggtaaa
     5461 tagagtttac ctcaaatagg gtagaacctc cttcatctgt cagttaataa cagccacttt
     5521 tacaatgccc tgtcgaaata aagtggcacg cccgattttt cactcatacc gattaaataa
     5581 tccatcgtct tccgagggaa cgcgatgccg cgcaccacgg tcagattcct caaaacgggg
     5641 aaaaccaaaa tatcctccat accgattccg ccgttgatgc cgtctgaagc accgtccatc
     5701 aaattttcca actcttgcaa atctgcgttt atccgttcga ggtattgggc ggttttattc
     5761 aaattggcgg aaaagctgcc gatgcttttc tcttttttgt ctgtaaaata tttcaccgct
     5821 tccggcgttg caaattcagg cagcccgatt ttgatcacgc gcggctgcac cagtttgtcg
     5881 ttgtatccgc ccaccttgtc cagccacgcc cgtatctcgg ggcggacttc gtctttcaga
     5941 cggtcttcgc ggtcgaaatg ccgcacaatg tccaaactct cgcccataaa cgaaccgtct
     6001 tctttttgca ggacgggcac ttgtttcgca ccgatcatac cgatcggcgt tgcctcgtcg
     6061 tcgtttgcca gcacggcttc ttcaacgtcc gcgccaaaca gcccggcagc catccgcgca
     6121 cgcacgcaaa acgggcaatg gtcgtaaata tacagtttca tcaaaatatt cctcgtcaac
     6181 ctgtcggtac cggctacctt aacaccccgc gccacccgaa acaagtttat cttcccgcct
     6241 atgcaccgta aataaataa
//
LOCUS       DAWHSK010000113         6142 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.113, whole genome shotgun sequence.
ACCESSION   DAWHSK010000113 DAWHSK010000000
VERSION     DAWHSK010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6142
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.113"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..2117)
                     /gene="clpA"
                     /locus_tag="WIC03_001321"
     CDS             complement(<1..2117)
                     /gene="clpA"
                     /locus_tag="WIC03_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HEZ3883816.1"
                     /translation="MLAPELEQILQQLYREARKAHYEFISLEHLLLVLIEEDASVHNV
                     LKLCGADLKVVSEQLAASVAENTPLIPEHLLDTVETRPTLGFQRVMQRAMVHTQSAGK
                     AAVEPLDVLVALMSETDSHTVYFLKLQSVTRFEVLRCIAHGSPDEDEDEDEDEDDGNY
                     SSDGMDDDNGNRTKPGKNPLSAYTVNLNAEVKAGRIDPLIGRKHEMERLVQILCRRRK
                     NNPLLVGEAGVGKTALAEGLAHQIVNGGIPDALKDAEVYALDMGSLLAGTKYRGDFEA
                     RVKSVLKQLEKIPHAILFIDEIHTIIGAGSTSGGTMDASNLLKPALAKGSLRCIGATT
                     YDEYRTIFDKDHALSRRFQKIDVVEPTVSETVQILRGLKPMFEAFHQVRYTQGALEAA
                     AELSARYINERFLPDKAIDVMDEAGAAQRILPKSKQKKVIGKAQIETVIAKVSRIPEK
                     TVSHDDKQVLQFLGRDLKNMVYGQENAIDALVAAVKMSRSGLALPDKPIGSFLFSGPT
                     GVGKTEAAKQLAYSLGVPLQRFDMSEYMERHAVSRLIGAPPGYVGFEQGGLLTEAVNK
                     QPHCVLLLDEIEKAHPDIFNVLLQVMDAGKLTDNNGKSADFRNVILIMTTNAGAESLS
                     RPSLGFTAKRERGDEMQAINKLFTPEFRNRLDAIIPFAPLSEPIIVKVVDKFLLQLEH
                     QLLDKKVETEFTPALHKYLAEKG"
     gene            complement(2119..2421)
                     /gene="clpS"
                     /locus_tag="WIC03_001322"
     CDS             complement(2119..2421)
                     /gene="clpS"
                     /locus_tag="WIC03_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HEZ3883817.1"
                     /translation="MTAQHQSDTLLRRLNTLPPKRYGVFLLNDDYTTMEFVVEILTEI
                     FMLGQEQAVAVMLLVHHEGKGLCGTYTRDIAQTKQQQVMQRAKAEGHPLQCIVEEI"
     gene            2664..2867
                     /locus_tag="WIC03_001323"
     CDS             2664..2867
                     /locus_tag="WIC03_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEZ3883818.1"
                     /translation="MATGIVKWFNDAKGFGFITPDEGGEDLFAHFSAINMEGFKTLKE
                     GQRVSFDVTTGPKGKQAANIQAA"
     gene            complement(3164..4495)
                     /gene="pmbA"
                     /locus_tag="WIC03_001324"
     CDS             complement(3164..4495)
                     /gene="pmbA"
                     /locus_tag="WIC03_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HEZ3883819.1"
                     /translation="MLFNHTASELLDLCRRTLDLAKAAGATAAEADFSESLGQSVSVR
                     LGEIEQIEFQQDKSLDITVYVGKRKGRASTADFSEKALQDTVKAAIDIARYTAEDDCA
                     GLADASLMAQHIGDPDLYHEWDLDTEAAVELAKQCEQAALNEDERIENSEGAAVQTGH
                     YQYVYGNTHGFAAHQQGTRHSISCSVVAADENGMQRDYWYDSSCRHQDMDSPTQIGQT
                     AAERTLRRLNSRSIPTGSYPVLFDATVSGGLIGHLVGALSGGSLYRQSSFLIDSIGKQ
                     VLPDFLSLREEPHIPRAFGSTYFDSEGVATRPRFVIRDGIVEGYFLSSYSARKLGMQT
                     TGNAGGAHNLYLNHTHETQSDLLKEMGTGLLVTELMGQGANTITGDYSRGAAGFWVEN
                     GVIAYPVHEITVAGRLQDMYRDIVGVADDALRRSSNKIGSILIAGMTVAGS"
     gene            4644..5156
                     /locus_tag="WIC03_001325"
     CDS             4644..5156
                     /locus_tag="WIC03_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HEZ3883820.1"
                     /translation="MKMFEQEDEWVSKTQMKKQMNGLQDLGMELTKLSSDTLKKIGLD
                     EDLYEAVVTYKKITSNGALKRQAQFIGRLMRDTDPAPIEAFLAKLRGDDAVHNAFLQR
                     VEQARVRLLADDGALTQFMSDFPHADAGKLRTLVRNTKKEQEQNKPPKNFRALFQELK
                     TVMENGDAEI"
     gene            5203..5922
                     /locus_tag="WIC03_001326"
     CDS             5203..5922
                     /locus_tag="WIC03_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883821.1"
                     /translation="MLFRKTTAAVLAAALMLNGCTAMMWGMNDPFSQTTAYKHVDKDQ
                     IRAFGVVAKDNAQLEKGSLVMMGGKYWFVVNPEDSAKLTGILKAGLDKPFQIVEDTPS
                     YARHQALPVKLESPGSQNFSTEGLCLRYDTDKPADIAKLKQLGFEAVKLDNRTIYTRC
                     VSAKGKYYATPQKLNADYHFEQSVPADIYYTVTEKHTDKSKLFANILYTPPPFLILDA
                     AGAVLALPVAALIAATNSSDK"
     gene            5991..>6142
                     /locus_tag="WIC03_001327"
     CDS             5991..>6142
                     /locus_tag="WIC03_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883822.1"
                     /translation="MSVKIQTRSVNTDVFNHLLTAGADPLIARLCASRGVQSPAELDD
                     KLASLLP"
BASE COUNT         1316 a         1556 c         1783 g         1487 t
ORIGIN      
        1 aaaccttttt ccgccagata tttgtgcaat gccggcgtga attcggtttc gacttttttg
       61 tcgaggagct ggtgttcgag ctggagcagg aatttgtcca cgactttgac gatgatgggt
      121 tcggacaagg gtgcaaacgg gataatcgcg tccaagcggt tgcggaactc gggcgtgaag
      181 agcttgttga ttgcctgcat ttcgtcgccg cgttcgcgct tggcggtaaa gccgaggctg
      241 gggcggctga ggctttccgc gcctgcgttg gtggtcataa tcaggatgac gttgcggaaa
      301 tcggcactct tgccgttgtt gtcggtcagt ttgcctgcgt ccatgacttg caggaggacg
      361 ttgaaaatgt cggggtgggc tttttcgatt tcgtccaaga gcaatacgca atgcggctgt
      421 ttgttgacgg cttcggtcaa aaggccgcct tgttcaaagc cgacgtagcc cggtggcgcg
      481 ccgatgaggc gcgatacggc atggcgttcc atgtattcgg acatatcaaa gcgttgcagc
      541 ggtacgccca gcgagtaggc aagctgtttg gcggcttcgg ttttgcccac gccggtcggg
      601 ccggagaaga ggaagctgcc tatcggtttg tcgggcaggg caaggccgga acgcgacatt
      661 ttgacggcgg caaccagcgc gtcgatggcg ttttcttgac cgtaaaccat gtttttcaaa
      721 tcgcgaccga ggaattgcag cacttgtttg tcgtcgtgcg acacggtttt ttcgggaatc
      781 cgcgaaactt tggcgatgac ggtttcgatt tgtgctttgc cgatgacttt tttctgtttg
      841 gatttgggca gaatccgttg cgccgcgcct gcttcatcca ttacgtcgat ggctttgtcg
      901 ggcaggaaac gctcgttgat gtagcgtgcg gagagttcgg cggcggcttc gagtgcgcct
      961 tgagtgtagc ggacttggtg gaaggcttca aacatcggtt tcaagccgcg caggatttga
     1021 acggtttcgg aaacggtggg ttcgaccacg tcgatttttt ggaagcggcg gcttaaggca
     1081 tggtctttgt cgaaaatggt gcggtattcg tcgtaggtgg tcgcgccgat gcagcgcagc
     1141 gaaccttttg ccagcgcggg tttgagcagg ttggacgcgt ccatggtgcc gccgctggtg
     1201 ctgcccgcgc cgatgatggt gtggatttcg tcgataaaca aaatggcgtg cgggattttt
     1261 tcgagctgtt tcaagacgga tttgacccgc gcttcaaagt cgccgcggta tttcgtgccc
     1321 gccaacagcg agcccatatc cagcgcgtac acttcggcat ctttaagcgc gtctggaatg
     1381 ccgccgttga cgatttgatg tgccaaacct tccgccagcg cggttttgcc cacgcccgct
     1441 tcgccgacca aaagcggatt gtttttgcgg cggcggcata ggatttgcac cagccgttcc
     1501 atttcgtgtt tgcgaccaat caaagggtcg atacggccgg ctttgacttc ggcgttgagg
     1561 ttgacggtgt acgccgataa agggtttttg cccggtttgg tgcggtttcc attatcgtcg
     1621 tccatgccgt ctgaagaata gttgccatcg tcttcatctt catcttcatc ttcatcttca
     1681 tcgggagagc cgtgggcaat acagcgcaaa acttcaaaac gcgtaaccga ttgcagcttg
     1741 aggaaataga cggtgtggct gtcggtttcg ctcatcagcg cgaccaaaac gtccaacggt
     1801 tcgactgcgg cttttccggc agactgggta tgcaccatcg cccgttgcat cacgcgttgg
     1861 aagccgagcg tgggccgggt ttcgaccgtg tctaaaaggt gttcgggaat caggggggtg
     1921 ttttcggcaa cgctggcggc gagctgttcg gacaccactt tcaaatccgc gccgcagagc
     1981 ttgaggacgt tgtggacgga ggcatcttct tcgatgagta ccaaaagcag atgctcgagg
     2041 ctgataaatt cataatgagc cttacgcgcc tcgcggtaaa gctgctgcaa aatctgttcc
     2101 aattcgggtg caagcatatt aaatctcctc gacaatacat tgcagcggat gcccttcggc
     2161 ttttgcccgc tgcatgactt gttgttgttt ggtttgggca atatcgcgcg tgtaagtgcc
     2221 gcacaggcct ttgccttcgt gatgaaccaa gagcatcacc gctaccgcct gttcttgtcc
     2281 gagcataaag atttcggtca ggatttcgac gacaaattcc atcgtggtgt aatcgtcgtt
     2341 caataggaaa acgccgtaac gttttggcgg cagggtgttc aggcggcgca agagcgtgtc
     2401 ggattggtgt tgcgcggtca tagtgtgtcc catttgaaag ccgcgttcag acggcatttt
     2461 tgttgtattt tcggtacttt tgcctatttt cccacttttt tgaaaacata gcttgacgtt
     2521 ttgtcttaac aaatgtaaaa agacggttaa gcaggattgg gcatccgccg agtatatcaa
     2581 gctggaagga acgccagacg gctcggtttg ccgtatgcct tgttttgctg attttgttaa
     2641 tttttgagta taggaagttt ctaatggcaa ccggtatcgt aaaatggttt aacgacgcta
     2701 aaggttttgg tttcatcacg cctgatgaag gcggcgaaga tttgttcgct cacttctcag
     2761 caatcaatat ggaaggtttc aaaaccctga aagaaggcca acgcgtctct ttcgacgtaa
     2821 ccaccggccc taaaggcaaa caggccgcca acattcaggc tgcttaattc ctgatgtacg
     2881 gtcaaatgta tatttgaaaa cggcgggaca ggcaatgtcc cgccgttttt gtctgccgtt
     2941 ttgccggcgg cggaaaaacc ccaatccccg cacgccttat cctgaacttg tgtgtaccct
     3001 gttgtggaca agtggcttag tattttgacg gataagggaa aatcagtgct gatgaaaaaa
     3061 tgtgcaatgt tgtcggcaaa aggcggtgcg gcataaaacg gcaaacgggc aggcacgggg
     3121 caaaacgtgc tgccttcgtc ttcagacggc atcggcaggg cgttcagctt ccggcaaccg
     3181 tcatccccgc aatcagaatc gagccgattt tgttggacga acgccgcaaa gcgtcatccg
     3241 ccacgccgac aatgtcgcgg tacatatcct gcaagcgtcc ggctacggta atctcgtgga
     3301 cggggtaggc aatcacgccg ttttccaccc aaaaacccgc cgcgccgcgc gagtagtcac
     3361 cggtaatggt gttcgcgccc tgtcccatca gttcggtaac caataatccc gtgcccattt
     3421 ctttcagcag gtctgattgc gtttcgtgcg tatggttcag ataaaggttg tgcgcgccgc
     3481 cggcgttgcc cgtggtctgc ataccgagtt tcctcgcgct gtaactgctg aggaaatagc
     3541 cttcgacgat gccgtcgcgg ataacgaaac gcggccgcgt ggcgacgcct tcggaatcga
     3601 aataggtgct gccgaaggcg cggggaatat gcggctcttc gcgcaggctg aggaaatcgg
     3661 gcagaacctg tttgcctatg ctgtcgatca ggaagctgct ttggcggtag agcgagccgc
     3721 cggacagtgc gccgacgagg tgtccgatca gacctcccga aacggtcgca tcgaatagga
     3781 cggggtagct tccggtcgga atgctgcggc tgttcagacg acgtaaggta cgttcggcgg
     3841 cggtttgacc gatttgcgtc gggctgtcca tgtcttgatg gcggcaggag gaatcgtacc
     3901 agtagtcgcg ctgcatgccg ttttcgtcgg cggcaacgac gctgcatgaa atgctgtggc
     3961 gtgtgccttg ctggtgtgcg gcaaaaccgt gggtgttgcc gtaaacgtat tggtaatgtc
     4021 cggtttgcac cgccgcccct tcggagtttt cgatgcgctc atcctcgttc agggcggctt
     4081 gttcgcattg ttttgccaac tcgacggcgg cttccgtatc caaatcccat tcgtggtaaa
     4141 ggtcggggtc gccgatgtgt tgcgccataa gcgaagcgtc tgccaaacct gcgcaatcgt
     4201 cttcggcggt gtagcgggcg atgtcgatgg cggctttgac ggtgtcttgc agggcttttt
     4261 cggagaagtc ggcagtgctg gcgcggcctt tgcgtttgcc gacgtaaacg gtaatgtcca
     4321 gcgacttgtc ctgctggaac tcgatttgtt cgatttcgcc cagccgcacg ctgacgcttt
     4381 gtcccaatga ttcgctgaaa tcggcttcgg cggcggtcgc gcccgctgct tttgccaagt
     4441 cgagcgtgcg gcggcagagg tcgagaagtt cggaagcggt gtggttgaac agcatagaaa
     4501 tctttcttgg tggagcgttg cggcatttta accgtttcgg gcggcagggg caaaagcgtg
     4561 ccgtttgcag ggcggacggt gcaaaatgcc gtctgaacgc ggaatcattc tgttaaaatg
     4621 cgcccgtcgg ataaaatttg agaatgaaga tgtttgaaca agaagacgaa tgggtcagca
     4681 aaacccaaat gaaaaagcag atgaacggtt tgcaggattt gggtatggag ctgaccaagc
     4741 tctcaagcga cacgttgaaa aaaatcggct tggacgaaga tttgtacgaa gccgtcgtta
     4801 cctataaaaa aatcacatcc aacggcgcgc tcaagcggca ggcgcaattt atcggcagac
     4861 tgatgcgcga taccgatccc gcgcccatcg aggcatttct tgccaagctg cgcggcgacg
     4921 atgcggtgca caatgccttt ttgcaacgcg tggaacaggc gcgcgtacgg ctgttggcag
     4981 acgacggcgc gttgacgcag tttatgtcgg attttccgca tgcggacgcg ggcaagctga
     5041 ggacgctcgt ccgcaacacc aaaaaagagc aggagcaaaa caaaccgccg aaaaacttcc
     5101 gcgcgctgtt tcaagaactg aaaaccgtga tggaaaacgg ggacgcggaa atttaggcat
     5161 attttcagac gacatccgcc gttatttaga ttggaggata aaatgttgtt ccgtaaaacg
     5221 accgccgccg ttttggcggc agccttgatg ttgaatggct gtacggcaat gatgtggggc
     5281 atgaacgacc cgttcagcca aacgaccgcc tataaacacg ttgacaagga tcaaatccgc
     5341 gccttcggtg tggttgccaa agacaatgcc caattggaaa agggcagtct ggtgatgatg
     5401 ggcgggaaat actggttcgt cgtcaatccc gaagattcgg cgaagctgac gggcattttg
     5461 aaggcagggc tggacaaacc cttccaaata gttgaggata ccccgagcta tgctcgccac
     5521 caagccctgc cggtcaaact cgaatcgcct ggcagccaga atttcagtac cgaaggcctt
     5581 tgcctgcgct acgataccga caagcctgcc gacatcgcca agctgaaaca gctcgggttt
     5641 gaagcggtca aactcgacaa tcgaaccatt tacacgcgct gcgtatccgc caaaggcaaa
     5701 tactacgcca caccgcaaaa actgaacgcc gattaccatt ttgaacagag tgtgcctgcc
     5761 gatatttatt acacggttac tgaaaaacat accgacaaat ccaagctgtt tgcaaatatc
     5821 ttatatacgc cccctccctt tttgatactg gatgcggcgg gcgcggtact ggccttgcct
     5881 gtcgcggcgt tgattgcagc cacgaattcc tcagacaaat gaacggcaat gccgtctgaa
     5941 aagccttcag acggcatttc aaacacatcc gcactgtaaa gttccacgct atgtccgtca
     6001 aaatccaaac ccgatccgtc aataccgacg ttttcaatca tttgctcacc gccggcgccg
     6061 atcctttaat cgcccggctt tgtgcctcgc gcggtgtgca aagtcctgcc gaattggacg
     6121 acaaactcgc ttccctcctg cc
//
LOCUS       DAWHSK010000114         6091 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.114, whole genome shotgun sequence.
ACCESSION   DAWHSK010000114 DAWHSK010000000
VERSION     DAWHSK010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6091
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.114"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(57..1460)
                     /gene="mtrE"
                     /locus_tag="WIC03_001328"
     CDS             complement(57..1460)
                     /gene="mtrE"
                     /locus_tag="WIC03_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MtrE"
                     /protein_id="HEZ3883823.1"
                     /translation="MDTTLKTTLTSVAAAFALSACTMIPQYEQPKVEVAETFKNDTAD
                     SGIRAVDLGWHDYFADPRLQKLIDIALERNTSLRTAVLNSEIYRKQYMIERNNLLPTL
                     AANANDSRQGSLSGGNVSSSYKVGLGAASYELDLFGRVRSSSEAALQGYFASTANRDA
                     AHLSLIATVAKAYFNERYAEEAMSLAQRVLKTREETYKLSELRYKAGVISAVALRQQE
                     ALIESAKADYAHAARSREQARNALATLINQPIPDDLPAGLPLDKQFFVEKLPAGLSSE
                     VLLDRPDIRAAEHALKQANANIGAARAAFFPSIRLTGTVGTGSAELGGLFKSGTGVWS
                     FAPSITLPIFTWGTNKANLDVAKLRQQAQIVAYESAVQSAFQDVANALAAREQLDKAY
                     DALSKQSRASKEALRLVGLRYKHGVSGALDLLDAERSSYAAEGAALSAQLTRAENLAD
                     LYKALGGGLKRDTQTDK"
     gene            complement(1515..4718)
                     /gene="mtrD"
                     /locus_tag="WIC03_001329"
     CDS             complement(1515..4718)
                     /gene="mtrD"
                     /locus_tag="WIC03_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MtrD"
                     /protein_id="HEZ3883824.1"
                     /translation="MAKFFIDRPIFAWVISIFIIAAGIFGIKSLPVSQYPSVAAPTIT
                     LHATYPGASAQVMEDSVLSVIERNMNGVEGLDYMSTSADSSGSGSVSLTFTPDTDENL
                     AQVEVQNKLSEVLSTLPATVQQYGVTVSKARSNFLMIVMLSSDVQSTEEMNDYAQRNI
                     VPELQRIEGVGQVRLFGAQRAMRIWVDPKKLQNYNLSFADIGSALSAQNVQISAGSIG
                     SLPAVRGQTVTATVTAQGQLGTAEEFGNVILRANTDGSNVYLKDVARVGLGMEDYSSS
                     TRLNGVNTTGMAVMLSNSGNAMATAKAVKERMATLEKYFPQGMSWKTPYDTSKFVEIS
                     IEKVIHTLIEAMVLVFVVMYLFLQNIRYTLIPTIVVPISLLGGFAFISYMGMSINVLT
                     MFAMVLVIGIVVDDAIVVVENVERIMAGEGLPPKEATKKAMGQISGAVIGITAVLISV
                     FVPLAMFSGATGNIYKQFALTMASSIAFSAFLALTLTPALCATMLKTIPKGHHEEKKG
                     FFGWFNKKFNSWTHGYEGRVAKVLRKTFRMMVVYIGLAVVGVFLFMRLPTSFLPTEDQ
                     GFVMVSVQLPAGATQERTNATLAQVTQLAKSIPEIENIITVSGFSFSGSGQNMAMGFA
                     ILKDWNERTAPGSDAVAIAGKLTGMMMGTLKDGFGIAVVPPPILELGNGSGLSINLQD
                     RNNTGHTALLAKRNELIQKMRASGLFDPSTVRAGGLEDSPQLKIDINRAAAAAQGISF
                     ADIRTALASALSSSYVSDFPNQGRLQRVMVQADEDARMQPADILNLTVPNKSGVAVPL
                     STIATVSWENGTEQSVRFNGYPSMKLSASPATGVSTGQAMAAVQKMVDELGGGYSLEW
                     GGQSREEAKGGSQTLILYGLAVAAVFLVLAALYESWSIPLAVILVIPLGLIGAAAGVT
                     GRNLFEGLLGSVPSFANDIYFQVGFVTVMGLSAKNAILIIEFAKDLQAQGKSAVEAAL
                     EAARLRFRPIIMTSFAFILGVVPLYIAGGASSASQRAIGTTVFWGMLIGTLLSVFLVP
                     LFYVVVRKFFKETAYEHEMAIRHASEAGITGSNDSQH"
     gene            complement(4730..5968)
                     /gene="mtrC"
                     /locus_tag="WIC03_001330"
     CDS             complement(4730..5968)
                     /gene="mtrC"
                     /locus_tag="WIC03_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit MtrC"
                     /protein_id="HEZ3883825.1"
                     /translation="MAFYAFKAMRAAALAAAVALVLSSCGKGGDAAQGGQPAGREAPA
                     PVVGVVTVHPQTVALTVELPGRLESLRTADVRAQVGGIIQKRLFQEGSYVRAGQPLYQ
                     IDSSTYEANLESARAQLATAQATLAKADADLARYKPLVAAEAVSRQEYDAAVTAKRSA
                     EAGVKAAQAAIKSAGINLNRSRITAPISGFIGQSKVSEGTLLNAGDTTVLATIRQTNP
                     MYVNVTQSASEVMKLRRQIAEGKLLAADGVIAVGIKFDDGTVYPEKGRLLFADPVVNE
                     STGQITLRAAVPNDQNILMPGLYVRVLMDQVAVDNAFVVPQQAVTRGAKDTVMIVNAQ
                     GGMEPREVTVAQQQGTNWIVTSGLKDGDKVVVEGISIASITGAKKVTPKEWAPSENQA
                     AAPQSGVQTASEAKTASEAE"
BASE COUNT         1382 a         1723 c         1622 g         1364 t
ORIGIN      
        1 aaagaaatgc cgtctgaaac cttatcgttt cagacggcat tttattgccc gaccggttat
       61 ttgtcggttt gggtatcccg tttcaatccg ccgccgagtg ccttgtacaa atcggcaagg
      121 ttttcggcgc gggtcagttg tgccgacaaa gccgcaccct ccgccgcata gctgctgcgt
      181 tccgcatcga gcaagtcgag cgcgccggat acgccgtgct tgtaacgcag gccgaccaag
      241 cgcaacgctt ctttagaggc gcggctttgt ttgcttaaag cgtcataggc tttatccagc
      301 tgctcgcgcg ccgccaatgc gtttgccacg tcttgaaatg cggattggac ggcggattca
      361 taggcaacga tttgtgcctg ttggcgcagc ttggctacat caaggttcgc cttgttcgta
      421 ccccaggtaa aaatcggcag ggtaatagac ggcgcgaacg accaaacgcc cgtgccgctt
      481 ttgaacaacc cacccaattc ggcagaaccc gtaccgacgg ttccggtcag gcggatggat
      541 gggaaaaagg cggcgcgtgc cgcaccgata ttggcgtttg cctgtttgag cgcgtgttcg
      601 gcggcgcgta tgtcgggacg gtcgagcaat acttcggaac tcaaaccggc cggcagtttt
      661 tcaacaaaaa actgcttgtc caacggcaaa ccggcgggca ggtcgtcggg tatcggttgg
      721 ttaatcaagg ttgccaaggc attgcgcgcc tgttcgcggc tgcgcgcggc atgggcataa
      781 tcggctttgg cagattcgat tagggcttcc tgctgacgta gggcgacggc ggaaatcacg
      841 cctgccttgt aacgtaattc ggacagcttg taggtttcct cgcgcgtttt caaaacacgt
      901 tgcgccaaag acatcgcttc ttcggcgtaa cgttcgttga aataggcttt ggcaacggtg
      961 gcaatcaggc tcaaatgtgc cgcatcgcgg ttggcggtgc tggcgaaata gccttgcagt
     1021 gccgcctcgc tgctgctgcg tacacgcccg aacagatcga gttcgtaaga tgccgcaccc
     1081 agtccgactt tgtagctgct gcttacattg ccgccgctca agctgccttg gcgcgagtcg
     1141 ttcgcattgg cggcaagcgt gggcaggagg ttgttgcgct caatcatgta ttgtttgcgg
     1201 tagatttcgc tgttcaatac ggcggtacgc aaactggtat tgcgctcgag tgcgatgtcg
     1261 atcagctttt gcaggcgcgg gtcggcaaaa tagtcatgcc aacctaaatc gaccgcacgg
     1321 atgccgctgt cggcggtatc gtttttgaac gtttcggcaa cttcgacttt gggctgctcg
     1381 tattggggaa tcatggtgca ggcggataat gcgaaggctg ctgcaacaga agtcaaggtg
     1441 gttttcaatg tagtatccat aaaaaagtcc tgatgccgtc tgaaaacccg taggcgttca
     1501 gacggcatgg ttgcttaatg ttggctgtcg ttcgaaccgg ttatgcccgc ttctgatgca
     1561 tgtctgatcg ccatttcatg ctcgtatgca gtttctttga agaatttgcg caccaccaca
     1621 tagaaaagcg gaacaaggaa cacggacaag agcgtgccga tgagcatccc ccagaatacg
     1681 gttgtaccaa tggcgcgctg gctggcagaa cttgcaccgc cggcaatata caggggaacc
     1741 acgcccaaaa taaaggcgaa cgaggtcatg ataatcggac ggaaacgcag gcgggctgct
     1801 tccaaagcgg cttcaaccgc gcttttccct tgcgcttgaa ggtctttggc aaactcgata
     1861 atcaaaatcg cgtttttcgc actcaaaccc atcacggtaa cgaaaccgac ttgaaagtag
     1921 atgtcgttgg cgaacgaggg aacgctgccc aacagtcctt caaacaggtt gcgcccggtt
     1981 acgcccgcag ccgcaccgat caaacccaac ggaatcacaa ggatgaccgc cagcggaatc
     2041 gaccagcttt cataaagcgc ggcaagtacc aaaaatacgg ctgcaaccgc caaaccgtac
     2101 aaaatcaggg tttgcgagcc gccttttgcc tcttcgcgcg actgtccgcc ccactccagg
     2161 ctgtaaccgc cgcccaattc gtcaaccatt ttttgaaccg ccgccatagc ctgcccggtg
     2221 gaaacgccgg ttgcaggcga agcggacagc ttcatcgaag gataaccgtt gaagcgtacg
     2281 ctctgttccg taccgttttc ccaagaaaca gtagcaatgg tggaaagcgg tacggcgacg
     2341 ccggatttgt tcggcacggt caggttcaaa atatcggcag gctgcatacg ggcatcctcg
     2401 tcggcctgca ccatcacgcg ttgcagacgg ccttggttcg ggaagtcgct gacataagac
     2461 gaactcagcg cacttgccaa tgcggtgcgg atgtcggcaa acgaaatgcc ttgcgccgcc
     2521 gcggcggcac ggttgatgtc gattttcaac tgcggcgagt cttccaaacc gccggcacgg
     2581 acggtgctgg ggtcaaacaa accgctggca cgcattttct gaatcaactc gttgcgcttc
     2641 gccagcaatg cggtatggcc ggtattgttg cggtcttgca ggttgatgct cagacccgaa
     2701 ccgttgccca actccagaat cggaggcggg acgacggcga tgccaaaacc gtctttaagc
     2761 gtccccatca tcatacccgt cagcttgccg gcaatcgcaa cggcatcgct gccgggcgcg
     2821 gtacgctcgt tccaatcttt caatatggca aaacccatcg ccatattctg accgctgccc
     2881 gaaaagctga agccggaaac ggtaatgatg ttttctattt caggaatgct tttcgccagt
     2941 tgggtaactt gcgccaaagt cgcattggtg cgctcttggg tcgctcctgc aggcagttgc
     3001 acgctgacca tgacgaagcc ttggtcttcg gtcggcagga atgaagtcgg cagacgcata
     3061 aacaggaaca cgcccacaac cgccaagccg atatagacaa ccatcatgcg gaaagtctta
     3121 cgcagcactt tggcaacccg gccttcgtaa ccgtgcgtcc aactgttgaa tttcttgtta
     3181 aaccagccga agaaaccttt tttctcttcg tgatgccctt tcgggattgt cttcaacatt
     3241 gtggcacaca aagcaggggt aagggtcagc gcaaggaagg cggagaatgc gattgatgac
     3301 gccatcgtca gggcaaactg tttgtaaata ttgcccgtcg ccccgctgaa catcgccaac
     3361 ggtacgaaca cggaaatcag aacggcggta ataccgatga ccgcgcccga aatctgaccc
     3421 atcgcttttt tggtcgcttc tttgggcggc aagccttcac ccgccataat gcgctcgacg
     3481 ttttcaacca ccacaatcgc gtcatcgacc acgatgccga tgaccaaaac catcgcaaac
     3541 atggtcagta cgttaatcga catgcccata taagagatga aggcgaaacc gcccaacagc
     3601 gaaatcggta cgacgatggt cggaatcagc gtataacgga tgttttgcag gaagagatac
     3661 attacgacaa acaccagcac catcgcttcg attaaagtat gaatcacttt ttcaatcgaa
     3721 atttcgacga atttggaagt atcgtaaggg gttttccagc tcataccctg aggaaagtat
     3781 ttttccaacg tcgccatgcg ttctttaacc gcctttgccg tcgccatcgc attgccgctg
     3841 ttggacagca tcaccgccat accggtggta tttacaccgt tcagacgggt tgaggaagaa
     3901 tagtcttcca tacccagtcc gacccttgcc acatccttca ggtaaacatt agaaccgtcg
     3961 gtattggcgc ggaggatgac gttgccgaat tcttctgccg tacccaactg cccttgcgcc
     4021 gttacggtag ccgtaaccgt ctgtccgcga acggcgggaa gcgaaccgat agaacccgct
     4081 gaaatctgga cgttctgggc ggacagcgca ctgccgatat cggcaaacga caaattgtag
     4141 ttttgcagtt tcttaggatc aacccaaatc cgcatcgcgc gttgcgcgcc gaacaggcgt
     4201 acctgcccca cgccttcgat acgctgcaac tcgggaacga tattacgctg cgcgtagtcg
     4261 ttcatctctt cggttgactg cacatccgac gaaagcatca caatcatcag gaaattggaa
     4321 cgcgccttgg atacggttac gccgtattgc tggacggttg ccggcagcgt gctcaatact
     4381 tcggaaagct tgttctgcac ttccacctgc gccagattct cgtcggtatc gggcgtaaag
     4441 gtcaggctca cgctgccgct gccgctcgaa tcggcggaag tggacatata atccaaacct
     4501 tccacgccgt tcatattccg ctcgatcacg gaaagcacgc tgtcttccat tacctgcgcg
     4561 gacgcacccg gataagtggc gtgcaaagta atggtcgggg cggcgacaga cggatattgc
     4621 gaaacaggca ggcttttgat gccgaaaata cccgccgcaa taatgaaaat cgaaataacc
     4681 cacgcaaaaa tagggcggtc gataaaaaat ttagccatcg atgccttcct tattccgctt
     4741 cagaagcagt tttggcttca gatgccgtct gaacgccgga ttgaggcgcg gcggcttggt
     4801 tttcagacgg cgcccattct ttgggcgtta cctttttcgc acccgttata ctggcgatac
     4861 tgatgccttc cacaaccacc ttgtccccgt ccttcagacc cgacgtaaca atccaattcg
     4921 taccctgctg ttgcgcaacc gttacctcgc ggggttccat accgccttgg gcattcacaa
     4981 tcatcacggt atctttcgca ccgcgcgtta ccgcctgctg cggcacaaca aatgcgttat
     5041 ccaccgccac ttggtccatc agcacgcgca catacagacc gggcatcagg atattctgat
     5101 cgttcggtac ggcggcgcgc agggtaatct gaccggtcga ttcgttgacg accggatcgg
     5161 caaacagcag gcggcctttt tcagggtaaa ctgtgccgtc gtcaaatttg atgccgaccg
     5221 caatcacacc atccgccgcc agcagtttgc cttcggctat ctgacggcgc aatttcatca
     5281 cttcggatgc agactgggta acgttcacat acatcggatt ggtttggcgg atggttgcca
     5341 gcacggtcgt atcgcccgca ttcaacagcg taccttcgga aactttggac tgaccgataa
     5401 agccggaaat cggcgcggta atgcgcgaac ggttcagatt gatgccggca gatttgatcg
     5461 ccgcctgtgc tgctttgaca cctgcctcgg cagaacgttt cgccgttacc gcagcatcgt
     5521 attcctgccg gctgacggct tcggcggcaa ccaaaggctt gtatcgcgcc aaatccgcat
     5581 ccgctttggc aagcgttgcc tgagccgttg ccagttgcgc gcgcgcgctt tccagatttg
     5641 cttcataagt ggaactgtcg atctgataca gcggctgtcc ggcacggaca taactgcctt
     5701 cttggaacag gcgtttttgg ataatgccgc caacttgcgc acggacatcg gcggtacgca
     5761 gcgattccaa acgtcccggc aactcgacgg tcaacgcaac ggtttgcgga tggacggtta
     5821 cgacaccgac gacgggagcc ggtgcttccc gacccgcagg ctgcccgccc tgcgccgcgt
     5881 ctccgccttt accgcaagac gacagtacca atgcaacggc ggcagccaac gcggccgcac
     5941 gcatcgcctt aaaagcataa aaagccatta tttatcctat ctgtctggtt tgatgtaaag
     6001 ggttttgcca atcaacaggc attcttatag tggattaaca aaaaccagta cggcgttgcc
     6061 tcgccttagc tcaaagagaa cgattctcta a
//
LOCUS       DAWHSK010000115         6002 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.115, whole genome shotgun sequence.
ACCESSION   DAWHSK010000115 DAWHSK010000000
VERSION     DAWHSK010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6002
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.115"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..421)
                     /locus_tag="WIC03_001331"
     CDS             complement(<1..421)
                     /locus_tag="WIC03_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HEZ3883826.1"
                     /translation="MLCPEKMSGMAGRHPYGVRSGLRRNGLKLWDIHFRMTRFIVARC
                     GLLFATLKGKTMKKMFVLFCMLFSCAFSLAAVNINAASLQELEALPGIGPAKAKAIAE
                     YRAQNGAFKSVDDLTKVKGIGPAVLAKLKDQASVGAPA"
     gene            complement(457..570)
                     /gene="rrf"
                     /locus_tag="WIC03_001332"
     rRNA            complement(457..570)
                     /gene="rrf"
                     /locus_tag="WIC03_001332"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(667..3558)
                     /locus_tag="WIC03_001333"
     rRNA            complement(667..3558)
                     /locus_tag="WIC03_001333"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            complement(3967..4042)
                     /locus_tag="WIC03_001334"
     tRNA            complement(3967..4042)
                     /locus_tag="WIC03_001334"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4007..4009),aa:Ala,seq:tgc)
     gene            complement(4048..4124)
                     /locus_tag="WIC03_001335"
     tRNA            complement(4048..4124)
                     /locus_tag="WIC03_001335"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4088..4090),aa:Ile,seq:gat)
     gene            complement(4226..5766)
                     /locus_tag="WIC03_001336"
     rRNA            complement(4226..5766)
                     /locus_tag="WIC03_001336"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         1306 a         1753 c         1288 g         1655 t
ORIGIN      
        1 gtgcgggcgc accgacggaa gcctgatcct tcagctttgc cagcaccgcg gggccgatac
       61 cctttacctt ggtcaaatcg tctacagact tgaacgcacc gttttgcgca cggtattccg
      121 caatggcctt cgccttcgcc gggcctatgc ctggcagcgc ctccaactcc tgcagcgaag
      181 ccgcattgat gtttaccgcc gcaagggaga aggcgcagga gaacagcata cagaacagca
      241 cgaacatttt cttcatggtt tttcctttaa gggttgcaaa caataaaccg catcttgcga
      301 cgataaaacg agtcattcta aaatgaatat cccaaagttt caagccgttc ctccgcaaac
      361 ccgaccggac gccgtacgga tgccgtcccg ccatccccga cattttttcc gggcaaagca
      421 aaaacccccg aataatcggg ggttttctga atgggtgttt ggcagtgacc tacttttgca
      481 tggaagaacc acactatcat cggcgctgag tcgtttcacg gtcctgttcg ggatgggaag
      541 gcgtgggacc aactcgctat ggccgccaaa cttaaactgt tacaaatcgg taaagcctta
      601 atcaatatat tcggtaatga ctgaatcagt cagtaagctt ttatctcttg aagttcttca
      661 aatgatagag tcaagcctca cgagcaatta gtatgggtta gcttcacgcg ttaccgcgct
      721 tccacacccc acctatcaac gtcctggtct cgaacgactc tttagtgcgg ttaaaccgca
      781 agggaagtct catcttcagg cgagtttcgc gcttagatgc tttcagcgct tatctcttcc
      841 gaacttagct acccggctat gcaactggcg ttacaaccgg tacaccagag gttcgtccac
      901 tccggtcctc tcgtactagg agcagccccc gtcaaacttc caacgcccac tgcagatagg
      961 gaccaaactg tctcacgacg ttttaaaccc agctcacgta ccactttaaa tggcgaacag
     1021 ccataccctt gggaccgact acagccccag gatgtgatga gccgacatcg aggtgccaaa
     1081 ctccgccgtc gatatgaact cttgggcgga atcagcctgt tatccccgga gtacctttta
     1141 tccgttgagc gatggccctt ccatacagaa ccaccggatc actatgtcct gctttcgcac
     1201 ctgctcgact tgtcggtctc gcagttaagc taccttttgc cattgcacta tcagtccgat
     1261 ttccgaccgg acctaggtaa ccttcgaact cctccgttac gctttgggag gagaccgccc
     1321 cagtcaaact gcctaccatg cacggtcccc gacccggatg acgggtctag gttagaacct
     1381 caaagacacc agggtggtat ttcaaggacg gctccacaga gactggcgtc tctgcttcaa
     1441 agcctcccac ctatcctaca caagtgactt caaagtccaa tgcaaagcta cagtaaaggt
     1501 tcacggggtc tttccgtcta gcagcgggta gattgcatct tcacaaccac ttcaacttcg
     1561 ctgagtctca ggaggagaca gtgtggccat cgttacgcca ttcgtgcggg tcggaactta
     1621 cccgacaagg aatttcgcta ccttaggacc gttatagtta cggccgccgt ttactggggc
     1681 ttcgatccga tgctctcaca tcttcaatta accttccagc accgggcagg cgtcacaccc
     1741 tatacgtcca ctttcgtgtt agcagagtgc tgtgttttta ataaacagtc gcagccacct
     1801 attctctgcg accctccggg gcttacggag caagtcctta accttagagg gcataccttc
     1861 tcccgaagtt acggtatcaa tttgccgagt tccttctcct gagttctctc aagcgcctta
     1921 gaattctcat cctgcccacc tgtgtcggtt tgcggtacgg ttcgattcaa actgaagctt
     1981 agtggctttt cctggaagcg tggtatcggt tacttcgtgt ccgtagacac tcgtcgtcac
     2041 ttctcggtgt taagaagacc cggatttgcc taagtcttcc acctaccggc ttaaacaagc
     2101 tattccaaca gcttgccaac ctaaccttct ccgtccccac atcgcatttg aatcaagtac
     2161 aggaatatta acctgtttcc catcgactac gcatttctgc ctcgccttag gggccgactc
     2221 accctacgcc gatgaacgtt gcgcaggaaa ccttgggctt tcggcgagcg ggcttttcac
     2281 ccgctttatc gctactcatg tcaacattcg cacttctgat acctccagca cactttacaa
     2341 tgcaccttca tcggcctaca gaacgctccc ctaccatgcc ggtaaaccgg catccgcagc
     2401 ttcggttata gatttgagcc ccgttacatc ttccgcgcag gacgactcga ccagtgagct
     2461 attacgcttt ctttaaatga tggctgcttc taagccaaca tcctggctgt ctgggccttc
     2521 ccacttcgtt taccacttaa tctatcattg gggaccttag ctggcggtct gggttgtttc
     2581 cctcttgaca acggacgtta gcacccgctg tctgtctccc gaggaaccac ttgatggtat
     2641 tctgagtttg ccatgggttg gtaagttgca ataaccccct agccataaca gtgctttacc
     2701 cccatcagtg tcttgctcga ggcactacct aaatagtttt cggggagaac cagctatctc
     2761 cgagtttgtt tagcctttca cccctatcca cagctcatcc ccgcattttg caacatgcgt
     2821 gggttcggtc ctccagtacc tgttacggca ccttcaacct ggccatggat agatcactcg
     2881 gtttcgggtc tacacccagc aactcatcgc cctattaaga ctcggtttcc ctacgcctcc
     2941 cctattcggt taagctcgct actgaatgta agtcgttgac ccattataca aaaggtacgc
     3001 agtcacacct ctagggtgct cccactgttt gtatgcatca ggtttcaggt tctgtttcac
     3061 tcccctcccg gggttctttt cgcctttccc tcacggtact ggttcactat cggtcgatga
     3121 tgagtattta gccttggagg atggtccccc catattcaga caggatttca cgtgtcccgc
     3181 cctacttttc gtacgcttag taccgctgtt gagatttcga atacgggact gtcacccact
     3241 atggtcaagc ttcccagctt gttcttctat ctcgacagtt attgcgtaca ggctcctccg
     3301 cgttcgctcg ccactacttg cggaatctcg gttgatttct tttcctccgg gtacttagat
     3361 ggttcagttc tccgggttcg cttctctaag tctatgtatt caacttagga tactgcacag
     3421 aatgcagtgg gtttccccat tcggacatcg cgggatcatt gctttattgc cagctccccc
     3481 gcgcttttcg caggcttaca cgtccttcgt cgcctatcat cgccaaggca tccacctgat
     3541 gcacttattc acttgactct atcatttcaa gaacttcttt gacttcgttt gcctacctgt
     3601 tgactaagta aaagcaaact tgaaattcct actttgataa agcttactgc tttgttgtgt
     3661 cttaatcctg ccttttgtgt ttcaggatta agtcgataca atcatcaccc aaatactgtg
     3721 tttgttttct tttctcttgt gagcgatttt ttatcctttg caaagaataa aaaatcaaaa
     3781 caaacgcttt gtctttgttt gttgatttcg gctttccaat ttgttaaaga tcgatgcgtt
     3841 cgatattgct atctactgtg caaatcaaaa cgagctgatt attatatcag cattttgttc
     3901 ttggtcaagt gtgatgtcgc cctgaatgga ttctgttcca ttcttccgtt ttgatttgta
     3961 cagtattggt ggaggcaaac gggatcgaac cgatgacccc ctgcttgcaa agcaggtgct
     4021 ctaccaactg agctatgccc ccgttattgg tgggtctggg aggacttgaa cctccgaccc
     4081 cacgcttatc aagcgtgtgc tctaaccagc tgagctacaa acccggattc tcttcttaag
     4141 cgaatcttgc cttcactcaa gcttcttccg catctttttc agtttaccga taagtgtgaa
     4201 tgcctaaagc ctcttctttc tctagaaagg aggtgatcca gccgcaggtt cccctacggc
     4261 taccttgtta cgacttcacc ccagtcatga agcataccgt ggtaagcgga ctccttgcgg
     4321 ttatcctacc tacttctggt atcccccact cccatggtgt gacgggcggt gtgtacaaga
     4381 cccgggaacg tattcaccgc agtatgctga cctgcgatta ctagcgattc cgacttcatg
     4441 cactcgagtt gcagagtaca atccggacta cgatcggttt tgtgagattg gctccacctc
     4501 gcggcttggc taccctctgt accgaccatt gtatgacgtg tgaagccctg gtcataaggg
     4561 ccatgaggac ttgacgtcat ccccaccttc ctccggcttg tcaccggcag tctcattaga
     4621 gtgcccaact gaatgatggc aactaatgac aagggttgcg ctcgttgcgg gacttaaccc
     4681 aacatctcac gacacgagct gacgacagcc atgcagcacc tgtgttacgg ctcccgaagg
     4741 cactcctccg tctctggagg attccgtaca tgtcaagacc aggtaaggtt cttcgcgttg
     4801 catcgaatta atccacatca tccaccgctt gtgcgggtcc ccgtcaattc ctttgagttt
     4861 taatcttgcg accgtactcc ccaggcggtc aatttcacgc gttagctacg ctaccaagca
     4921 atcaagttgc ccaacagcta attgacatcg tttagggcgt ggactaccag ggtatctaat
     4981 cctgtttgct acccacgctt tcgggcatga acgtcagtgt tatcccagga ggctgccttc
     5041 gccatcggta ttcctccaca tctctacgca tttcactgct acacgtggaa ttctacctcc
     5101 ctctgacaca ctcgagtcac ccagttcaga acgcagttcc cgggttgagc ccggggattt
     5161 cacatcctgc ttaagtaacc gtctgcgccc gctttacgcc cagtaattcc gattaacgct
     5221 cgcaccctac gtattaccgc ggctgctggc acgtagttag ccggtgctta ttcttcaggt
     5281 accgtcatca gccgctgata ttagcaacag ccttttcttc cctgacaaaa gtcctttaca
     5341 acccgaaggc cttcttcaga cacgcggcat ggctggatca ggcttgcgcc cattgtccaa
     5401 aattccccac tgctgcctcc cgtaggagtc tgggccgtgt ctcagtccca gtgtggcgga
     5461 tcatcctctc agacccgcta ctgatcgtcg ccttggtagg cctttacccc accaaccagc
     5521 taatcagata tcggccgctc gaatagcgca aggcccgaaa gtcccctgct ttctctctca
     5581 agacgtatgc ggtattagct gatctttcga tcagttatcc cccactactc ggtacgttcc
     5641 gatatgttac tcacccgttc gccactcgcc acccaagaag caagcttctc tgtgctgccg
     5701 tccgacttgc atgtgtaaag catgccgcca gcgttcaatc tgagccagga tcaaactctt
     5761 atgttcaatc tctaactttt taacttctgg tctgcctcaa agaaaccaac aggacaatgt
     5821 tcaaaacatt atcttgtctg tctttcaaac agtgtgaggc tcaaggcact cacacttatc
     5881 ggtaatctgt tttgttaaag agcgtttgaa ttataaagta tttcttccgc ctgtcaagat
     5941 atctctcgat atccccaaca ttctgtgcta tacttttcag ttcgtccgcc acttctgcag
     6001 ca
//
LOCUS       DAWHSK010000116         5943 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.116, whole genome shotgun sequence.
ACCESSION   DAWHSK010000116 DAWHSK010000000
VERSION     DAWHSK010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5943
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.116"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            173..1624
                     /gene="cobA"
                     /locus_tag="WIC03_001337"
     CDS             173..1624
                     /gene="cobA"
                     /locus_tag="WIC03_001337"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEZ3883827.1"
                     /translation="MNYFPIFANLAGRPVLVVGGGAVAARKISLLLKAGAEVRVAAKH
                     LNAELSALAAENKILWLAEEFRAEHIRTVFLIIAASSDQALNRRVFHLAESCQKPVNV
                     VDDRDHCSFIFPSVIDRNPVQIAVSSSGSAPVLARLLRERLEALLPPSLGDMAEISGR
                     WRDAVKDKLKSVTERRRFWEKQFNGRFAALVKNRQNTLAERELAGQLEQSRQNDQGGS
                     VSLVGAGPGDAGLLTLKGLQEIQQADVVLYDALVSDGILSLVRRDAERIFVGKRARGD
                     RTPQEDTNALMVRLAREGRRVVRLKGGDPFVFGRGGEELETLARHQIPFSVVPGITAA
                     VGATAYAGIPLTHRDYAQSAVFVTGHRKADAPDIEWQTLARSRQTLVIYMGALKAALI
                     AERLQQHGRSPDTPAAVISQGTLPAQKTATGTLANLAELAETAPNPALIVIGEVVGLH
                     EKLAWFGENGEGENRVGQAYPALGGLNAGQRAA"
     gene            1635..2375
                     /locus_tag="WIC03_001338"
     CDS             1635..2375
                     /locus_tag="WIC03_001338"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEZ3883828.1"
                     /translation="METTLLKPALWQIPHIGSGSETALAEKTETLKQRLHRIAGSHRD
                     ARFASSLAAEDMVITDLIAGENLNIGIFTLDTGLLHAETLNLLDRIERVYPHMQIKRF
                     QPIREDALHYVESKGRFAFYDSVEARRECCRIRKIEPLNRAIAGADAWLTGQRREQSA
                     TRTELPFAEYDAGRGIGKYNPIFDWSEHDVWAYILANNVPYNDLYRQGFPSIGCDPCT
                     RPVKAGEDIRAGRWWWEGRNSKECGLHK"
     gene            2413..3336
                     /gene="cysD"
                     /locus_tag="WIC03_001339"
     CDS             2413..3336
                     /gene="cysD"
                     /locus_tag="WIC03_001339"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEZ3883829.1"
                     /translation="MTKTEPNNAQLDWLESESIHIIREVAAECENPALLFSGGKDSVV
                     LLALACKAFRLGSRPVKLPFPLVHIDTGHNYPEVIAFRDAQAAKLNARLIVGRVEDSI
                     AKGTVVLRKETDSRNAAQAVTLLETIEANGFDALMGGARRDEEKARAKERIFSFRDEF
                     GQWNPKAQRPELWSLYNTRLHKGENMRVFPISNWTELDIWQYIARENLELPPIYYSHR
                     REVVRRRGLLVPVTPLTPKMPSETSEILDVRFRTVGDISCTCPVESTASTPTEIIRET
                     AVADISERSATRLDDQASEAAMEKRKKEGYF"
     gene            3375..4661
                     /locus_tag="WIC03_001340"
     CDS             3375..4661
                     /locus_tag="WIC03_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit 1"
                     /protein_id="HEZ3883830.1"
                     /translation="MTAQHQTPLRFITAGSVDDGKSTLIGRLLYDSKALLSDQIKTLE
                     SGKSNGLKETIDFSILTDGLEAEREQGITIDVAYRYFSTAKRKFIIADTPGHEQYTRN
                     MVTGASTASAAVVLVDASQLDFGAQPLQLLPQTKRHSAILRQLNCPHIVVAVNKMDLL
                     DYSEDKFNAIVEAYRRLAEQLGLKDVHFVPVSALLGDNIVYPGGNMPWYKGEPLLSIL
                     ENLPGADETGRSAADFYFPVQLVVRQDADKADDFRGYQGRIERGSVAVGQTVRIEPNG
                     LTAEVSEIITPKGEVAQAFAGEAATLRLDRDIDVSRGDLFVDKNSPLAPQKHLEATLC
                     WFDERPLNTARKYLLKHGTQTVPAKVGEIESVLDVRTLEQEAGAESLKMNDIAKVRIN
                     LQKPVTATPYAENTAAGSFILIDEATYGTVAAGMIL"
     gene            4658..>5943
                     /locus_tag="WIC03_001341"
     CDS             4658..>5943
                     /locus_tag="WIC03_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HEZ3883831.1"
                     /translation="MSEHDMQNTNPPLPPLPPEITQLLSGLDAAQWAWLSGYAWAKAG
                     NGASAGLPALQTALPTAEPFSVTVLSASQTGNAKSVADKAADSLEAAGIQVSRAELKD
                     YKAKNIAGERRLLLVTSTQGEGEPPEEAVVLHKLLNGKKAPKLDKLQFAVLGLGDSSY
                     PNFCRAGKDFDKRFEELGAKRLLERVDADLDFTASANAWTDNIAALLKEEAAKNRATP
                     APQATPPADLQTASEGRYCKAAPFPAALLANQKITARQSDKDVRHIEIDLSGSDLHYL
                     PGDALGVWFDNDPALVREILDLLGIDQATEIQAGGKTLPVASALLSHFELTQNTPAFV
                     KGYAPFADDDELDRIAADNAVLQGFVQSTPIADVLHRFPAKLTAEQFAGLLRPLAPRL
                     YSISSSQAEVGDEVHLTVGAVRFEHEGRARAGGASG"
BASE COUNT         1429 a         1810 c         1642 g         1062 t
ORIGIN      
        1 gaaaaccgcc gcatccgcag ccgccctcat atagctttca gcttctaaca aatgcctttt
       61 tattatttaa ccgggaaaat ccttttgtct aaaatgaccc gcattccgac aaaaatgccg
      121 cgacgcaccg tatcggaaac aaggtgcggg caaacagcaa aggagtcagc cagtgaatta
      181 tttccctatt ttcgccaacc tcgccggccg ccccgtattg gtcgtaggcg gcggcgcggt
      241 cgccgcacgc aaaatcagcc tgctgctgaa ggcgggcgca gaggtcaggg ttgccgcaaa
      301 acacctgaat gccgaactct ccgcgttggc ggcggaaaac aaaatcctgt ggcttgccga
      361 agaatttcgt gccgaacaca tccgcacagt tttcctcatc attgcggcaa gcagcgacca
      421 agccctcaac cggcgcgttt tccatcttgc cgaaagctgc caaaagccgg tcaacgtggt
      481 ggacgaccgc gaccattgca gcttcatctt cccgtcggtt atcgaccgca acccggttca
      541 gattgccgtt tccagctccg gcagcgcgcc cgtcctcgcc cgactgctgc gtgaaaggct
      601 ggaagccctg ctgccgccgt ctttgggcga tatggcggaa atttcaggaa ggtggcgcga
      661 tgccgtcaag gacaaactga aatccgttac cgaacgccgc cgcttttggg aaaagcagtt
      721 taacggacgt ttcgccgccc tcgtcaaaaa ccggcaaaac acccttgccg aacgggaatt
      781 ggcaggccaa ttggaacaaa gtcgtcaaaa tgatcaaggc ggctccgtct cgctggtcgg
      841 cgcgggtccc ggcgatgcgg ggctgctgac gctcaaaggg ctacaggaaa tccagcaggc
      901 agacgtggtg ctttacgacg cgctggtttc agacggcata ctgtccctcg tccgccgcga
      961 tgcggaacgg atttttgtcg gcaagcgcgc ccggggcgac cgcaccccgc aagaggacac
     1021 caatgcgctt atggtcaggc tggcccgcga aggcaggcgc gtggtgcgtt tgaaaggcgg
     1081 agaccctttc gtgttcggac gcggcggcga agaattggaa accttggcac gccatcagat
     1141 tccgttttcg gtcgtccccg gcattaccgc cgccgtcggc gcgaccgcct atgccggcat
     1201 cccgctcacg caccgcgatt atgcccaaag tgcggttttc gtaaccggcc accgcaaggc
     1261 ggatgcgccc gacatcgaat ggcagaccct cgcccgcagc cgccagacct tggtgattta
     1321 tatgggtgcg ctcaaagccg ccctgattgc cgaacggctg caacagcacg gacgctcgcc
     1381 cgacacgccg gcagccgtca tcagccaagg cacgttgccg gctcaaaaaa ccgcaaccgg
     1441 cacgctcgcc aaccttgccg aactcgccga aaccgcgccg aatccggcat tgattgtcat
     1501 aggcgaagtg gtggggctgc acgaaaaact cgcctggttc ggtgaaaacg gcgaaggaga
     1561 aaaccgggtc gggcaggcgt atcccgcatt gggcggactg aatgccggac aacgggcggc
     1621 ataaggcggg aaaaatggaa acgacactgc tcaaacccgc cctgtggcag ataccgcata
     1681 tcggatccgg cagcgaaacc gcccttgcgg aaaaaacgga aaccctcaaa caacgcctgc
     1741 accggattgc cggcagccac cgggatgcac ggtttgccag cagccttgct gcggaagata
     1801 tggtaattac cgaccttatc gccggagaaa acctcaatat cggtattttt actttggata
     1861 cggggctgct gcacgcggaa actttaaacc tgttggatag gattgaacgt gtttatccgc
     1921 atatgcaaat caagcgtttc caaccgatac gggaagatgc cctgcattat gtggaaagca
     1981 aaggcaggtt tgccttttac gacagcgttg aagcgcgccg cgaatgctgc cgcatccgaa
     2041 aaatcgaacc gctcaaccgc gccattgcag gcgcggacgc ctggcttacc gggcaacgcc
     2101 gcgaacaatc cgccacacgc acggaactgc cgtttgccga atacgatgcc ggacggggca
     2161 tcggcaaata caacccgatt ttcgactggt cggaacacga cgtgtgggca tacatcctcg
     2221 ccaacaatgt gccttacaac gatttgtacc ggcaaggatt tcccagcata ggctgcgacc
     2281 cctgcacccg ccccgtcaag gcgggcgaag acatccgcgc agggcggtgg tggtgggaag
     2341 gcagaaacag caaagaatgc gggctgcaca aataattcat aaatatcaga cagaagaaat
     2401 aaggaatatg gtatgacgaa aaccgaaccg aacaacgccc aactcgactg gttggaatcc
     2461 gaatccatcc acattatccg cgaagtggcg gcagaatgcg aaaaccccgc cctgctcttt
     2521 tccggcggca aagattccgt ggtcctgctc gccctcgcct gcaaagcctt ccggctgggc
     2581 agcaggccgg taaaactgcc cttcccgctg gtgcatatcg acacgggaca caattatccc
     2641 gaagtgattg ccttccgcga cgcgcaggcg gcaaaactca atgcccgcct gatagtgggg
     2701 cgcgtggaag actccatcgc caaaggcaca gtagttttac gcaaagaaac cgattcgcgc
     2761 aatgcggcgc aggcggttac gctgttggaa accatcgagg caaacggctt tgacgcgctg
     2821 atgggcggcg cgcggcgcga cgaagaaaaa gcccgcgcca aagaacggat tttctcgttc
     2881 cgcgacgagt tcggacaatg gaatccgaag gcgcaacgcc ccgagctgtg gtcgctgtac
     2941 aacaccaggc tgcacaaagg cgaaaatatg cgcgtcttcc cgatttccaa ctggacggaa
     3001 ctcgacatct ggcaatacat cgcccgcgaa aacctcgaac tgccgccgat ttattacagc
     3061 cacaggcgcg aagtggtcag acgcaggggg ctgctggtcc ccgttacgcc gcttacgccg
     3121 aaaatgccgt ctgaaacctc cgaaatcctt gatgtccgct tccgcaccgt cggcgacatc
     3181 agctgcacct gcccggtaga aagcaccgcg tccacgccga cggagattat cagggaaacg
     3241 gcggttgccg acatttccga acgcagcgcg acccggctgg acgatcaggc aagcgaggcg
     3301 gcaatggaaa aacgcaaaaa agaaggctat ttctaaattt caaaaaccct ttaagcagat
     3361 atgaggaaga aaatatgacg gcacaacacc aaaccccgct ccgcttcatt accgccggca
     3421 gcgtcgatga cggcaaaagc accctaatcg gacgactgct ctacgacagc aaagccctgc
     3481 tgtccgacca aatcaaaacc ttggaatccg gcaaaagcaa cggtttgaaa gaaaccatag
     3541 acttctccat cctgaccgac ggactggaag ccgaacgcga acagggcatt acgatcgatg
     3601 tggcataccg ctatttttcc acggcgaaac ggaaattcat catcgccgac acgccggggc
     3661 acgaacaata cacgcgcaat atggttacgg gcgcaagcac cgcttcggcg gcagtcgtac
     3721 tggttgacgc atcccaactg gatttcggcg cgcagccctt gcagcttctg ccgcagacca
     3781 aacgccattc cgccatcctc cgccaactca actgcccgca tattgtggtg gcggtcaaca
     3841 aaatggacct gctggattac agcgaagaca aattcaacgc cattgtggag gcataccgcc
     3901 gacttgccga acagctcggt ttgaaagacg tgcatttcgt accggtgtcc gcactcttgg
     3961 gcgacaacat cgtttacccc ggcggcaata tgccttggta taaaggcgaa cccttattgt
     4021 ccattttgga aaacctgccg ggggcggacg aaaccggtcg ttccgctgcc gatttttatt
     4081 tccccgtcca actggtcgtc cgccaagatg ccgacaaggc cgatgatttc cgaggctatc
     4141 aggggcgcat cgaacgcggt tcggtcgccg tcgggcaaac cgtccgcatc gaaccgaacg
     4201 ggctgaccgc cgaagtgtcc gaaatcatca cgccgaaagg ggaagtggcg caggcttttg
     4261 ccggcgaggc ggcaaccctc cggctggacc gcgacatcga tgtttcgcgc ggcgaccttt
     4321 ttgtcgataa aaattccccg ctcgccccgc aaaaacatct ggaagccacg ctttgctggt
     4381 ttgacgaacg tccgctcaac accgcgcgca aatacctgct caagcacggc acgcaaaccg
     4441 tgccggcaaa agtcggggaa attgaaagcg ttttggatgt ccgcacgctg gaacaagagg
     4501 ccggcgcgga atccttgaag atgaacgaca tcgccaaagt ccgcatcaac cttcaaaaac
     4561 ccgttacggc aacgccttat gcggaaaaca ccgccgccgg ttcgtttatc ctgatagacg
     4621 aagcgacata cggcactgtt gcggcaggta tgattttatg agtgaacacg atatgcagaa
     4681 cacaaatccg ccattaccgc ctctgccgcc cgaaatcacg cagctcctgt cggggctgga
     4741 cgcggcacaa tgggcgtggc tgtccggcta cgcttgggca aaagcaggaa acggggcatc
     4801 tgcaggactg cccgcgcttc agacggcatt gccgacggca gaaccttttt ccgtaaccgt
     4861 cctttccgcc tcgcaaaccg gcaatgcgaa atccgttgcc gacaaagcgg cggacagcct
     4921 ggaagccgcc ggcatccaag tcagtcgcgc cgaactgaaa gactataagg cgaaaaacat
     4981 cgccggcgaa cgccgcctgc tgctggttac gtccacccaa ggcgaaggcg aaccgccgga
     5041 agaagccgtc gtgctgcaca aactgctgaa cggcaaaaaa gccccgaaat tggacaaact
     5101 ccaatttgcc gtactgggtt tgggcgacag ttcctatccg aatttctgcc gggcgggcaa
     5161 agatttcgac aaacgttttg aagaattggg ggcgaaacgc ctgctcgaac gcgttgatgc
     5221 ggatttggac tttaccgcct ccgcaaacgc ctggacagac aatatcgccg cactcttaaa
     5281 agaagaagcc gcaaaaaacc gggcaacgcc cgcgccgcag gcaacgcccc ccgccgacct
     5341 tcagacggca tcggagggca ggtactgcaa ggcagccccc tttcccgccg ccctgctggc
     5401 caatcagaaa atcaccgccc gccaatccga taaagacgtg cgccacatcg aaatcgattt
     5461 gagcggttcg gatttgcact acctcccggg cgacgcgctc ggcgtttggt ttgacaacga
     5521 tccggcactg gtcagggaaa tcctagacct gctcggcatc gatcaggcaa cggaaataca
     5581 ggcgggcgga aaaaccctgc cggttgcctc cgcactgtta tcccattttg aactcacgca
     5641 aaacaccccc gcctttgtca aaggctatgc cccgttcgcc gatgatgacg aactcgaccg
     5701 tattgctgcc gacaacgccg ttttgcaagg ctttgtgcaa agcacgccga ttgccgatgt
     5761 gctgcaccgc ttccctgcaa aactgacggc ggaacaattc gccggcctgc tgcgcccgct
     5821 tgcgccgcgc ctgtattcga tttcctcgtc gcaggcggaa gtgggggacg aagtgcacct
     5881 gaccgtcggc gcggtgcgtt ttgaacacga agggcgcgcc agagcgggcg gcgcatcggg
     5941 ttt
//
LOCUS       DAWHSK010000117         5804 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.117, whole genome shotgun sequence.
ACCESSION   DAWHSK010000117 DAWHSK010000000
VERSION     DAWHSK010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5804
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.117"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            259..>5804
                     /locus_tag="WIC03_001342"
     CDS             259..>5804
                     /locus_tag="WIC03_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HEZ3883832.1"
                     /translation="MYAGKITLISTDRDASVRNQGQWFASAGNVAVNAEGKLVNTGMI
                     AATGENHAVSLHARNVHNSGTVASQDDANIHSQTLDNSGTVLSSGQLTVRNLGRLKNH
                     NNGTIQAARLDMSTGGLDNTGNITQTGSQALDLVSAGKFDNSGKIGVSDVPQTGLNPN
                     PSVIPQIPSTATGSGSSTVLASKPGSNNPVSPTAPAKNYAGGRIQTTGAFDNAGSINA
                     GGQIDIAAQNGLENSGSLNAAKLRVSGDSFNNTVKGKLQAHDLAVNTQTAKNSGHLLT
                     LTGKIDNRELHNAGEIAANNLTLIHSGRLSNDKKGNIRAAHLQLDTAGLHNAGNILAD
                     SGTVTTKNNLRNTGKVSVARLNTEGQTLDNTHGHIEAETVNIQSQQLTNQSGHITATE
                     QLTINSRNVDNQNGKLLSANQAQLAVSDGLYNQHGEIATNRQLSIHDKNQNTLALNNA
                     DGTIQSAGNVSLQAKSLANNGTLTAGNKLDIALTDDFVVERDLTAGKQLNLSIKGRLK
                     NTHTLQASHTLKLNAGNIDNQVTGKIIGGEQTDITSEQHVDNRGLINSDGLTHIGAGQ
                     TLTNTGTGKIYGNHIALDAQILLNREETTEGSTKAGAIAARKRLDIGAKEIHNQEGAL
                     LSSEGIFAVGNRLDEQHHAAGMADTFVNGSAGLEVQGDALMSVRNMQNINNHFKTETY
                     LAKAEKQVRDYTVLGQNTYYQAGKDGLFDNSQGQKDQTTATFHLKNGSRIEANQWHVR
                     DYHIETYKERIIENRPAHITVGGDLTASGQNWLNKDSRIVVGGRIITDDLNQKEITNQ
                     STTGKGRTDAVGTQWDSVTKKGWYSGRKRQRRTERNHTPYHDTQLFTHDFDTPVSVIQ
                     QNAASPSFQPAASAIKLIDGVSTAAVNGQRIHTGNVVSLNNATVTLPNSSLYTTHPDN
                     KGWLVETDPQFADYRRWLGSDYMLQQLQLDTNHLHKRLGDGYYEQKLVNEQIHQLTGY
                     RRLDGYRSDEEQFKALMDNGLTAAKTFGLTPGIALSAEQVARLTSDIVWMENQTVTLS
                     DGSTQTVLVPKVYALARKGDLNTSGGLISAEQVLLKLQNGNLTNSGTIAGRQAVLIQA
                     RNINSNGNIQADQIGLKAEKSINIDGGQVQAGRLLTAQAQNINLNGTTQTSGNERNGN
                     TAIDRMAGINVVGSHTEQVDNRTSDGILSLHAGNDINLNAATVSNQVKGGTTQITAGN
                     NLNLGTIHTEHREAYGTLDDENHRHVRQSTEVGSSIRTQNGALLRAGNDLKIRQGELE
                     AEEGKTVLAAGRDVTISEGRQITELDTSVSGKSKGILSSTKTQDRYRFSHDEAVGSNI
                     GGGKMIVAAGQDINVRGSNLISDKGIVLKAGHDIDISTAHNRYTGNEYHESKKSGVMG
                     TGGLGFTIGNRKTTDDTDRTNIVHTGSIIGSLNGDTVTVAGNRYRQTGSTVSSPEGRN
                     TVTAKSIDVEFANNRYATDYAHTQEQKGLTVALNVPVVQAAQNFIQAAQNVGKSKNKR
                     VNAMAAANAAWQSYQAAQQMQQFAPSSSAGQGQNNNQSPSISVSITYGEQKSRNEQKR
                     HYTEAAASQIIGKGQTTLAATGSGEQSNINITGSDVIGHAGTALIADNHIRLQSAKQD
                     GSEQSKNKSSGWNAGVAVKIGNGIRFGITAGGNIGKGKEQGGNTTHRHTHVGSTAGQT
                     TIRSGGDTTLKGAQLIGKGIQADTRNLHIESVQDTETYQSKQQNGNVQVTVGYGFSAS
                     GSYRQSKVKADHASVTEQSGIYTGEDGYQIKVRDNTDLKGGIITSTRSAESKGKNRFQ
                     TATLTHRDIQNHSRYEGRSFGIGGSFDLNGGWDGTVTDKQGRPTDRISPAAGYGSDGD
                     SKNSTTR"
BASE COUNT         1837 a         1493 c         1337 g         1137 t
ORIGIN      
        1 gaccttagcg gctttaagat aaggcaaggc aatgttgtaa tcgccggaca cggtttggat
       61 gcccgtgata ccgatttcac gcagatattg agccatgcgg ttaagattga tgggcctgtg
      121 tggggtaaag atgtgcgtgt gtcggcaggg aaaaatgatg tctcagctga tggctctatc
      181 cggtcttcac actcccctgc tacgaatata aattccgaca actcactgta tgccatcgat
      241 acaggggcat tgggcggtat gtatgccggt aagatcaccc tgatcagcac tgatcgtgat
      301 gcatccgtgc gcaatcaggg gcaatggttt gcctcagccg gcaatgtggc agtgaatgct
      361 gagggtaaac tggtcaacac aggcatgatt gcagcgacgg gagaaaatca tgcggtttca
      421 cttcatgccc gcaatgttca taatagcggt acggttgcct cacaggatga tgccaatatt
      481 cacagccaga cgctggacaa ttcaggtacg gtcttatcct caggtcaatt gactgtccgt
      541 aatttaggcc gtctgaaaaa ccataacaac ggtacgatcc aggctgcacg cttagatatg
      601 tcaacaggtg gtttggataa cacaggtaat attactcaaa caggttcaca agcattggat
      661 ttggtatctg caggcaaatt cgataacagt ggcaagattg gtgtaagtga cgttccacag
      721 accggtttga atcccaatcc atcagtcata ccacagattc cgagtactgc aacaggttca
      781 ggcagcagca ctgtcttggc atctaagcct ggttcaaaca atcccgtttc acctacagca
      841 cctgcaaaaa actacgccgg aggacgcatt caaacaacag gagcatttga caatgcagga
      901 tcaatcaatg caggtgggca aattgacatt gccgcccaaa acggtttgga aaattcgggt
      961 agtctgaatg cggctaaact acgagtatca ggcgattcat ttaacaatac ggtaaaaggc
     1021 aaactccagg cacacgatct ggctgttaac actcaaactg ctaaaaacag cggtcactta
     1081 ttaactctaa ccggcaagat tgacaaccgt gaactgcata atgccggaga aattgccgcc
     1141 aacaatctga cactcattca ttcgggccgc ttgagcaatg ataaaaaagg caatattcga
     1201 gctgcacatt tacagcttga taccgccggt ttacataatg caggtaacat tcttgccgat
     1261 agtggaaccg ttaccaccaa gaataatctt cgcaatacag gaaaagtttc tgttgcacga
     1321 ctaaataccg aaggtcagac tctagataat acgcacggac atatagaggc tgaaacggtt
     1381 aacatccaaa gtcagcaact gactaaccaa agtggccata ttactgctac cgaacaactg
     1441 actatcaata gtcgaaatgt agacaaccaa aacggcaaac tcctatctgc aaaccaagca
     1501 caattagctg tttcagacgg cctatacaac caacatggtg aaattgccac caaccggcag
     1561 ttgtctattc acgataaaaa tcaaaacact ttggcgttaa acaatgcgga tggcacgatt
     1621 caatctgccg gtaatgtatc gctacaagcc aaatcactcg ccaacaatgg cacattaaca
     1681 gccggtaaca aactggatat tgctttgacg gacgatttcg tcgtagagcg cgacctcact
     1741 gcaggcaaac aattaaatct aagcataaaa ggccgtctga aaaataccca taccctacaa
     1801 gcaagccata cgctcaaact caatgccggc aatatagata accaagttac aggcaaaatt
     1861 attggtggag aacaaacgga catcacatcc gaacagcatg ttgacaacag gggcttgatc
     1921 aacagcgacg gtttgaccca catcggtgca ggtcaaaccc tgaccaacac cgggacaggc
     1981 aaaatctatg gcaaccatat tgccctggac gcgcaaatac tgcttaaccg ggaagaaacg
     2041 acggaaggca gtaccaaagc gggggcaata gctgcaagga aacgtttgga tattggagcg
     2101 aaagagattc ataaccaaga aggtgcccta ctatccagcg aaggtatttt tgccgtaggt
     2161 aatcgactgg atgaacaaca tcatgcggca ggcatggccg atacctttgt taatggcagt
     2221 gccggtttgg aagtacaagg tgatgcattg atgtccgttc ggaatatgca gaatatcaat
     2281 aatcacttta aaacagagac atacttagcc aaagcagaaa agcaagttcg tgactacacc
     2341 gtactggggc aaaataccta ctatcaggca ggaaaagacg gtttattcga caactcgcaa
     2401 ggacaaaaag accaaactac tgctacgttc catttaaaaa atggttctcg tattgaggcc
     2461 aaccaatggc atgtccgaga ctaccacatc gagacttata aagaacgcat catcgaaaac
     2521 cggccggcac acattactgt gggcggtgat ttgactgcct caggtcaaaa ttggctgaac
     2581 aaagacagcc ggattgtagt aggcgggcgt attatcactg atgatttaaa ccagaaagaa
     2641 attaccaatc aaagtacaac aggcaaaggt cgcacagatg ctgtcggcac acagtgggat
     2701 tcagttacaa aaaaaggatg gtacagcggt agaaaaagac aacgccgtac tgaaagaaac
     2761 catactcctt accatgatac ccaactattt acccacgact tcgacacgcc tgtatccgtc
     2821 atccaacaga atgccgcctc cccttccttt caacccgctg catctgcaat caaactgatt
     2881 gacggagtat ccacggcagc cgtcaatggt cagcgcatcc ataccggtaa tgtggtctcg
     2941 ttaaataacg ctactgttac tctgcctaac agcagcctct ataccaccca tcctgacaat
     3001 aaaggctggt tggttgaaac cgatcctcaa tttgcagact accgccgctg gttgggcagc
     3061 gactacatgt tgcaacaact gcaattggac accaatcatc tacacaaacg gctaggcgac
     3121 ggctactacg aacaaaaact tgttaatgaa caaatccatc agttaacagg ctaccgccga
     3181 ctcgacggct acaggagtga tgaagaacaa ttcaaagctc tgatggacaa cggccttact
     3241 gctgccaaaa cattcggtct caccccaggt atcgccttga gtgcagagca agttgcccgc
     3301 ttaacttcag atatcgtttg gatggaaaat caaaccgtca ccctgtctga cggttcgact
     3361 caaaccgtac tggttcctaa agtctatgcc ctggcgcgca aaggtgatct caatacctcc
     3421 ggtggcctga ttagtgccga acaagtctta cttaaactgc aaaacggcaa cctgactaac
     3481 agcggtacca ttgcggggcg acaggccgta ctcatccagg cacggaatat taacagcaac
     3541 ggtaacattc aagccgacca aatcggctta aaagctgaaa aaagtatcaa tatcgacggc
     3601 gggcaggtac aagcaggcag actgctgact gcccaagcgc aaaatatcaa ccttaacggt
     3661 acaacccaaa cttccggtaa tgaacgtaac ggcaataccg ccatcgatcg tatggccggc
     3721 attaacgtgg tcggaagcca tactgagcaa gtagataaca gaacttcaga cggcatccta
     3781 tccctgcatg ccggcaacga tatcaacctc aatgcggcca ccgtctctaa ccaagttaaa
     3841 ggcggcacta cccaaattac cgccggcaat aatctcaacc tcggcaccat tcataccgaa
     3901 catcgcgaag cctatggtac attagatgac gagaaccatc gccatgtccg ccaaagtacc
     3961 gaagtcggca gcagtatccg cacgcaaaac ggcgcactgc ttagagccgg taacgactta
     4021 aaaatccgcc aaggcgaact ggaggccgaa gaaggcaaaa ccgtccttgc cgcgggacgt
     4081 gatgtcacta tcagcgaagg acgccaaata accgaactgg atacctcggt aagcggaaaa
     4141 agcaaaggca tcctttccag taccaaaaca caggatcgct accgcttcag tcatgatgaa
     4201 gcagtcggca gcaacatcgg cggcggcaaa atgattgttg cagccgggca ggatatcaat
     4261 gtacgcggca gcaaccttat ttctgataag ggcattgttt taaaagcagg acacgacatc
     4321 gatatttcta ctgcccataa tcgctatacc ggcaatgaat accacgagag caaaaaatca
     4381 ggcgtcatgg gtactggcgg attgggcttt actatcggta accggaaaac taccgatgac
     4441 actgatcgta ccaatattgt ccatacaggc agcattatag gcagcctgaa tggagacacc
     4501 gttacagttg caggaaaccg ctaccgacaa accggcagta ccgtctccag ccccgagggg
     4561 cgcaataccg tcacagccaa aagcatagat gtagagttcg caaacaaccg gtatgccact
     4621 gactacgccc atacccagga acaaaaaggc cttaccgtcg ccctcaatgt gcccgttgtc
     4681 caagctgcac aaaacttcat acaagcagcc caaaatgtgg gcaaaagtaa aaataaacgc
     4741 gttaatgcca tggctgcagc caatgctgca tggcagagtt atcaagcagc ccaacaaatg
     4801 caacaatttg ctccaagcag cagtgcggga caaggtcaaa acaacaatca aagccccagt
     4861 atcagtgtgt ccatcaccta cggcgaacag aaaagtcgta acgagcaaaa aagacattac
     4921 accgaagcgg cagcaagtca aattatcggc aaagggcaaa ccacacttgc ggcaacagga
     4981 agtggggagc agtccaatat caatattaca ggttccgatg tcatcggcca tgcaggtact
     5041 gccctcattg ccgacaacca tatcagactc caatctgcca aacaggacgg cagcgagcaa
     5101 agcaaaaaca aaagcagtgg ttggaatgca ggcgtagccg tcaaaatagg caacggcatc
     5161 aggtttggaa ttaccgccgg aggaaatatc ggtaaaggta aagagcaagg gggaaatact
     5221 acccaccgcc acacccatgt cggcagcaca gccggacaaa ccaccatccg cagcggcggg
     5281 gataccaccc tcaaaggtgc gcagctcatc ggcaaaggca tacaggcaga tacgcgcaac
     5341 ctgcatatag aaagtgttca agatactgaa acctatcaga gcaaacagca aaacggcaat
     5401 gtccaagtta ctgtcggtta cggattcagt gcaagcggca gttaccgcca aagcaaagtc
     5461 aaagcagacc atgcctccgt aaccgagcaa agcggtattt ataccggaga agacggctat
     5521 caaatcaaag tcagagacaa cacagacctc aagggcggta tcatcacctc cacccgaagt
     5581 gccgaaagca aagggaaaaa ccgctttcag acggccaccc tcacccatcg cgacattcaa
     5641 aaccacagcc gctacgaagg cagaagcttc ggcataggcg gcagtttcga cctgaacggc
     5701 ggctgggacg gcacggttac cgacaaacaa ggcaggccta ccgacaggat aagcccggca
     5761 gccggctacg gcagcgacgg agacagcaaa aacagcacca cccg
//
LOCUS       DAWHSK010000118         5716 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.118, whole genome shotgun sequence.
ACCESSION   DAWHSK010000118 DAWHSK010000000
VERSION     DAWHSK010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5716
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.118"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(147..1190)
                     /locus_tag="WIC03_001343"
     CDS             complement(147..1190)
                     /locus_tag="WIC03_001343"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEZ3883833.1"
                     /translation="MSKKIKVGIVGATGYTGVELLRLLAAHPDVEVAAVTSRSEAGTA
                     VADYFPSLRGVYGLAFQTPDEAGLEQCDIVFFATPNGIAMKDAPRLIEQGVRVIDLSA
                     DFRIRDIPTWEHWYGMTHAAPDLVSQAVYGLSELNREAVAQARLVANPGCYPTCVSLP
                     LVPLLRQCRLKPGMPLIADCKSGVSGAGRKGNVGSLLCEVGDNFKAYGIAGHRHLPEI
                     RQTIAGLQDGIAEGFVFTPHLAPMIRGMHATVYLHLSDGICPETILRDYYRDSLFVDI
                     LPAGSTPETRSVRGANLCRISIQQAAQSDVWVVLSVIDNLVKGAAGQAVQNMNIMFGL
                     KETHGLDAIPLLP"
     gene            complement(1187..1468)
                     /locus_tag="WIC03_001344"
     CDS             complement(1187..1468)
                     /locus_tag="WIC03_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883834.1"
                     /translation="MKTLVLLLLSFSTTTAFAAYGLGLGQAPKYPADFRAYGYVYSGR
                     QGWVLKTEADAIKLDTLFKRFVYRPSTANQNPVGQRSDGIPANHTKEKP"
     gene            1836..2990
                     /locus_tag="WIC03_001345"
     CDS             1836..2990
                     /locus_tag="WIC03_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEZ3883835.1"
                     /translation="MSIHLDFGISPKTFRQTYLYQKPKLFKGAARNLEAASWKDINEI
                     YQRADPTAPLFYLRKKGAIVPKEEYVESFDALGKTRYRFIKSVIYEHMKNGASLVYNH
                     INNEPFSDHISRQVARFAGAHTIVSGYLAFGSDESYKNHWDTRDVYAIQLFGKKRWQL
                     TAPDFPMPLYMQQTKDTDISIPEHIDMDIILEAGDVLYIPRGWWHRPIPLGCETFHLA
                     VGTFPPNGYNYLEWLMKKFPTIESLRHSFSDWEQDRTRINDTAAQIAAMIADPVNYEA
                     FSEDFLGKERTDTAFHLEQFANPNATPLSDDVRLRLNANNLDTLEKGYLIGNGMKISV
                     DELGKKVLEHIGKNEPLLLKNLLVNFNQGKHEEVRKLIYQLIELDFLELL"
     gene            3410..3670
                     /locus_tag="WIC03_001346"
     CDS             3410..3670
                     /locus_tag="WIC03_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin transporter"
                     /protein_id="HEZ3883836.1"
                     /translation="MERKNNIIELSIENLELIYGGGLGERILEGGANGAQIGGSIGAG
                     IGLGVGIVSGGIPAGPAIILGGTVGAAVGAVTGMGVAAYEYY"
     gene            3830..4456
                     /locus_tag="WIC03_001347"
     CDS             3830..4456
                     /locus_tag="WIC03_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein"
                     /protein_id="HEZ3883837.1"
                     /translation="MNRPKQPFFRPEVTVARQTSLTGKVILTRPLSFSLWTTFASISA
                     LLIILFLIFGNYTRKTTVEGQILPASGVIRVYAPDTGTITTKFVEDGEKVKAGDKLFA
                     LSTSRFGAGGSVQQQLKTEAVLKKTLAEQELGRLKLIHENETRSLKATVERLENQKLH
                     ILQQIDGQKRRIRLAEDQWQKRHWADRNCRDWAWYPPIWRRAANLFIL"
     gene            4408..4608
                     /locus_tag="WIC03_001348"
     CDS             4408..4608
                     /locus_tag="WIC03_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883838.1"
                     /translation="MVSSDLAKSSEPVYLVKIKPDKSTITAYGEEKPLQIGMTLEADI
                     LHEKRRLYEWVLELIYSMSGKL"
     gene            complement(4805..4912)
                     /locus_tag="WIC03_001349"
                     /pseudo
     CDS             complement(4805..4912)
                     /locus_tag="WIC03_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            5508..>5716
                     /locus_tag="WIC03_001350"
     CDS             5508..>5716
                     /locus_tag="WIC03_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEZ3883839.1"
                     /translation="MPHLQNLSLGLKKKLPVILQTEISECGLACLAAVAGFHGFHTDL
                     RALRSKYCPRPLQNSPKSPKCLGGN"
BASE COUNT         1531 a         1231 c         1407 g         1547 t
ORIGIN      
        1 attcccgctt ttgcgggaat gacgggattt tagatttttg ttttcgcgga ataatgagcc
       61 aaaagccgtc tgaacctaaa aaaccgccac tttcagatgg catcaaaaaa ccgcaggcac
      121 gatacctgcg gtttgccgtt tgcgtttcag gggagcaggg ggattgcgtc caagccgtgt
      181 gtttccttca gtccgaacat gatgttcata ttttgcaccg cctgacccgc cgcgcctttg
      241 acgaggttgt cgatgacgga aaggacgacc cacacatcgg attgcgccgc ctgttggatg
      301 ctgatgcggc agaggtttgc gccgcgcacg ctgcgggttt cgggtgtcga accggcgggc
      361 aggatgtcca cgaacaggct gtcgcggtaa tagtcgcgca ggatggtttc ggggcagatg
      421 ccgtctgaaa ggtggaggta aacggtggcg tgcataccgc gtatcatcgg cgcgaggtgc
      481 ggcgtgaaca cgaatccttc ggcgatgccg tcctgaagcc cggcgatggt ctgcctgatt
      541 tcgggcaggt ggcggtgtcc agctatgccg taggctttga agttgtcgcc gacttcgcac
      601 aacagcgaac cgacattgcc tttcctgccc gcgccggaca cgccggattt gcagtcggca
      661 atcagcggca taccgggctt cagacggcat tgccgcaaca gcggcacgag cggcagggat
      721 acgcaggtcg ggtagcagcc ggggttggcg acgaggcgtg cctgtgcgac ggcttcgcgg
      781 ttgagttcgc tcaatccgta cacggcttgg gaaacgaggt cgggggcggc gtgggtcatg
      841 ccgtaccagt gttcccaggt cggaatgtcc cgtatgcgga agtcggcgga aaggtcgatg
      901 acgcgcacgc cctgttcaat caggcgcggc gcgtctttca tggcgatgcc gttgggcgtg
      961 gcgaagaaga cgatgtcgca ttgttccaaa cctgcctcgt cgggcgtttg gaaggcgagg
     1021 ccgtacacgc cgcgcaaact cggaaagtaa tcggcaactg cagttcccgc ttcgctgcgg
     1081 ctggttacgg cggcgacttc gacatcggga tgggcggcaa gcaggcgcag cagttccacg
     1141 cccgtgtagc ccgtcgcgcc gacaatgccg actttgattt ttttactcat ggtttttcct
     1201 ttgtgtggtt ggcgggtatg ccgtccgaac gctgtccgac ggggttttga ttggcggtgg
     1261 aaggacggta cacaaagcgt ttgaaaagcg tgtctaattt aatggcatcc gcctctgttt
     1321 ttaaaaccca gccctgccgt ccggaataaa cataaccgta ggcgcgaaag tcggcaggat
     1381 atttcggtgc ctgccccaaa cccaaaccgt atgcggcgaa agcggtggtc gtggagaagg
     1441 aaagcaggag gaggacgagg gttttcataa ggttccaaac cggacggggc gatgaagccc
     1501 gattatagca aaaggtcgtc tgaaaaccgt tttcagacga ccttttattt aattacaata
     1561 aaaatgttaa caacaaaaaa caaaccgctt tttttcgttt ggaaacaaaa caaattttta
     1621 tagtggatta aatttaaacc agtacagcgt tgcctcgcct tgccttgccg tactatctgt
     1681 actgtctgcg gtttcgtcgc cttttcctga tttttgttaa tccactataa ctaaaattgt
     1741 caacaattcc ttgacgcaca cacaaaaaaa actttaatat ttgtaaatat atgtaacatt
     1801 ataaatttta aatattttct gagtaagggg aagtaatgag catccatctc gacttcggca
     1861 ttagtcctaa aacgttccga cagacttatc tgtatcaaaa gcccaagctc tttaaaggag
     1921 cggctcggaa tctcgaagcc gcatcttgga aagatatcaa cgagatatac caacgagcag
     1981 acccaaccgc accgctgttt tatctgcgta aaaaaggtgc aattgttcct aaagaagaat
     2041 acgtcgaaag tttcgacgct ttgggcaaaa ctcgctaccg ttttattaaa tccgttatct
     2101 acgaacatat gaagaatggt gcgtcgttag tctataacca tattaacaac gagccgtttt
     2161 cagatcatat ttcccgtcaa gtcgcccgct ttgctggcgc acatactatt gtcagtggat
     2221 atcttgcttt tggcagcgac gaatcttata aaaaccattg ggacacccgc gatgtgtatg
     2281 ccatccagct tttcggcaag aaacgttggc aacttactgc ccctgatttc cctatgccat
     2341 tgtatatgca acagactaaa gatactgata tttccattcc tgaacatatc gatatggata
     2401 ttatccttga agcaggtgat gtcctctaca tcccgcgcgg ttggtggcac agacctatcc
     2461 cgctcggctg tgaaaccttc cacctcgctg tcggtacctt tccgcccaac ggctataatt
     2521 acctcgagtg gctaatgaag aaattcccca cgatagaaag tctgcgccac agtttctcag
     2581 actgggagca agataggacg cgtatcaacg atactgccgc acaaattgct gccatgattg
     2641 ccgaccccgt caattacgaa gccttcagtg aagacttcct cggcaaagaa cgcaccgata
     2701 ccgcttttca tctcgaacag ttcgcgaatc ccaacgctac tccgctttca gacgacgtca
     2761 ggttgagact aaatgccaat aatttggata cgttggaaaa ggggtatttg attgggaatg
     2821 ggatgaagat aagcgtagat gaattgggga aaaaagtttt agaacacatc ggtaagaatg
     2881 aaccgttatt gttgaaaaat ctactggtta acttcaatca gggaaaacat gaagaagtta
     2941 ggaagttgat ttatcagttg atagagttag attttctgga acttttgtga gggattctat
     3001 gaaaaactgg aagcaattta catttttcgt aatattagta atagcttgtt atcaagtgct
     3061 atatttttta tcagatatgt ttcttctcga ctacattaac aaatatagtt ggaatttgaa
     3121 ttttattcag ggtactttga attttttttc aatatatctg ccatatgttt ttgttagccg
     3181 aatttttagg aatactaacc aatgaaagac taggctatga aatcaaaatt tatgtataag
     3241 ttactggtac ttgcaaagta cctgtatatt ttaatttgta tatcaatacc aatcgttttt
     3301 atttatgaaa gctaccaagg agatgatgtt gattttatga tttatattca tgcttttatt
     3361 attttttcca tattgggtgt attgactcaa tattctacgt ctaatttttt tggaaaggaa
     3421 aaataacatc attgaactat ccattgagaa tctggagcta atttatggcg gaggcttagg
     3481 ggagagaatc cttgaaggag gagcaaatgg tgcacaaata ggaggatcaa ttggtgcagg
     3541 tattggtctg ggtgtcggta tagtttctgg tggtattcct gccggaccgg ctattattct
     3601 cggtggcaca gttggtgctg ctgttggtgc tgtaactggt atgggtgttg cagcatatga
     3661 gtattactaa gaaactcagc ccaaagggac agtaacggtc ggtccgattt ctgcttttgc
     3721 tgtggacaaa tcaggaaacg gctacactga cggtacagat tactgctgat atttctcatc
     3781 tttcagacga tctctagggg tcgtctgaaa ctttcttaac ttcaatttta tgaatagacc
     3841 caagcaacct ttcttccgcc ctgaagtcac agttgcccgg caaaccagcc tgacgggtaa
     3901 agtgattctg acacgaccgt tgtcattttc cctatggacg acatttgcat cgatatctgc
     3961 gttactgatt atcctgtttt tgatatttgg taactatacg cgaaagacaa cagtggaggg
     4021 acaaatttta cctgcatcgg gcgtaatcag ggtgtatgca ccggatacgg ggacaattac
     4081 aacgaaattc gtggaagatg gagaaaaggt caaggctggc gacaagctat ttgcgctttc
     4141 gacctcacgt ttcggcgcag gaggtagcgt gcagcagcag ttgaaaacgg aggcagtttt
     4201 gaagaaaacg ttggcagaac aggaactggg tcgtctgaag ctgatacacg agaatgaaac
     4261 gcgcagcctt aaagcaactg tcgaacgttt ggaaaaccag aaactccata ttttgcaaca
     4321 gatagacggt cagaaaaggc gcattagact tgcggaagat cagtggcaaa aacggcactg
     4381 ggcagacagg aattgtcggg attgggcatg gtatcctccg atttggcgaa gagcagcgaa
     4441 cctgtttatc ttgtgaaaat aaaacccgac aaatcaacca tcactgcata cggtgaggaa
     4501 aaaccgctgc aaatcggcat gacgttggaa gcagacatcc tgcacgagaa acggcgattg
     4561 tacgaatggg tattggagct gatttatagt atgtcgggca aactgtaaga cttgaggctg
     4621 aaagataaag aagttgtctg aaaccttttc agacgaccag aagacaatat ataaaaacac
     4681 aaacttcaaa actgcctgaa accatcccct tttacaggca ctccgtctgt aaaagcaagg
     4741 atagggcatc tgcccttttg attgtttgat taacgataca aggagtttca aaatgagagt
     4801 tttatagtgg attaaattta aaccagtaca gcgttgcctc gccttgccgt accatttgta
     4861 ctgtctgcgg cttcgtcgcc ttgtcctgat ttaaatttaa tccactatat gtgttcatga
     4921 aatgacttgg gtcggaggct caggtaatgc gtaacaaagc tcatattatt gcgaaatttg
     4981 cgaatctgca gggcttaacg atacgggaaa tcctgataaa tctttaggat tgccaaacaa
     5041 tacgttcagt aatccgcctg gttggggagc tacaatcgga gctttagcag gtagccgcat
     5101 agggatgcct gaatttggta cgtttgcgag ccatgccatt gaaaatttcg actggtcatg
     5161 gtatcgacgt tatagggaaa ttgccgaaac gattgaacga gaatattcag gcggtttgcc
     5221 ttaatagttg aggaggtcat gatgtttgcc aaacattatc aattcatcgc actcggcatc
     5281 atgctgcttc tttatatgtt gattctctat acgaccgatt tttccaatct gacgtattgg
     5341 atgctgtttt ttatctgttt tattacagga aaaatattag ctcgtttgtt agagaaaagc
     5401 tttaaataaa atagcagcta gtcgcaaaag gtcgtctgaa accttttcag gcggcctttc
     5461 taaaatacat ccaacttcct aatccctatt tttcaaaaag gaaatctatg ccccatctgc
     5521 aaaacctgtc tttgggctta aagaaaaagc tgcctgttat cctgcaaaca gaaatatcag
     5581 aatgcggctt ggcatgtctg gcggctgtgg cgggatttca tggtttccat acggatttac
     5641 gcgcactgcg ttcaaaatac tgtccgagac ctttgcaaaa ttccccaaaa tcccctaaat
     5701 gtcttggtgg gaattt
//
LOCUS       DAWHSK010000119         5666 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.119, whole genome shotgun sequence.
ACCESSION   DAWHSK010000119 DAWHSK010000000
VERSION     DAWHSK010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5666
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.119"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            16..207
                     /locus_tag="WIC03_001351"
     CDS             16..207
                     /locus_tag="WIC03_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883840.1"
                     /translation="MFQQRTAHQAPHCVLPERDYPPIRFYRYKQTGFNRKGMGIKSIS
                     DTSRAMPSENQSPLSDGIV"
     gene            284..1231
                     /locus_tag="WIC03_001352"
     CDS             284..1231
                     /locus_tag="WIC03_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HEZ3883841.1"
                     /translation="MNILSKISSFIGKTFSLWAALFAAAAFFAPDTFKWAGPYIPWLL
                     GIIMFGMGLTLKPSDFDILFKHPKAVIIGVIAQFAIMPATAWLLSKLLNLPAEIAVGV
                     ILVGCCPGGTASNVMTYLARGNVALSVAVTSVSTLISPLLTPAIFLMLAGEMLEIQAA
                     GMLMSIVKMVLLPIVLGLIVHKVLGSKTEKLTDALPLVSVAAIVLIIGAVVGASKGKI
                     MESGLLIFAVVVLHNGIGYLLGFFAAKWTGLPYDAQKTLAIEVGMQNSGLAAALAAAH
                     FAAAPVVAVPGALFSVWHNISGSLLATYWAAKAGKHKKP"
     gene            complement(1383..2507)
                     /gene="rluD"
                     /locus_tag="WIC03_001353"
     CDS             complement(1383..2507)
                     /gene="rluD"
                     /locus_tag="WIC03_001353"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HEZ3883842.1"
                     /translation="MQNTSFDNESDYSDDSDFASASETENRIGLTVPLELAGGRLDAV
                     LAKLLPDYSRSRLTSWIKEGAVIVNDKPSQPKDKMIGGEQICVTVRPSEENLAFVPEP
                     MALDIVYEDDTVIVVNKPAGLVVHPAAGNWTGTLLNGLLAHCPELSQVPRAGIVHRLD
                     KETSGLMVVAKTLPAQNSLVRQLQERTVKRIYRAVANGIVPFDGKIETQIGRDPHNRL
                     KMAVVKFGGKPAVTHVKVLERYLTHSYIECSLETGRTHQIRVHMREANHPLAADPVYG
                     NPRHPCGDTVKEAVKSLGARQALHAYRLSFTHPESGETVSFEAPIPDDIYHLLSVLRL
                     EAGLDSSLSNEEEWQDKFGADDDDDWNEDDYDVEVVYVRE"
     gene            2506..3309
                     /locus_tag="WIC03_001354"
     CDS             2506..3309
                     /locus_tag="WIC03_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEZ3883843.1"
                     /translation="MKKILLTVSLGLALSACATQGTVDKDAQITQDWSVEKLYAEAQD
                     ELNSSNYTRAVKLYEILESRFPTSRHARQSQLDTAYAYYKDDEKDKALAAIERFRRLH
                     PQHPNMDYALYLRGLVLFNEDQSFLNKLASQDWSDRDPKANREAYQAFAELVQRFPNS
                     KYAADATARMVKLVDALGGNEMSVARYYMKRGAYIAAANRAQKIIGSYQNTRYVEESL
                     AILELAYQKLGKPQLAADTRRVLETNFPKSPFLTHAWQPDDMPWWRYWH"
     gene            complement(3367..5586)
                     /locus_tag="WIC03_001355"
     CDS             complement(3367..5586)
                     /locus_tag="WIC03_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEZ3883844.1"
                     /translation="MFRRWFLPCWVVGAAASFALPVVPHWLFWLAAFAVFAVFARRFA
                     FAGLMLCVLAGAAYGVFRTEAALSSQWWAEAVSGVPLTVEVADMPRSDGRRVQFAAKA
                     VDSGGRTFDLLLSDYKRREWAVGSRWRITARVHPVVGELNLRGLNREAWALSNGIGGA
                     GTVGADRVLLHGGSGWGIAVWRSRISRNWQQADADGGLSDGIGLMRALSVGEQSALRP
                     ELWQAFRPLGLTHLVSISGLHVTMVAVMFAWLAKRLLACSPRLPARPRAWVLAAGCAG
                     ALFYALLAGFSVPTQRSVLMLAAFAWAWRRGRLSAWATWWQALAAVLLFDPSAVLGVG
                     TWLSFGLVAALIWACSGRLHEGKRQTALRGQWAASVLSLVLLGYLFASLPLVSPLVNA
                     VAIPWFSWVLTPLALLGSVVPFAPLQQLGAFLAEYTLRFLVWLADVSPEFAVASAPLP
                     LLVLVVCAALLLLLPRGLGLRPWAVLLLAGFVFYRSPGVPENEVAVTVWDAGQGLSVS
                     VQTANHHLLFDTGTAAAAQMGIVPSLNASGIRYLDYLVLSHHDSDHDGGFQAVGKIPN
                     GGIYAGQPEFYEGARHCAEQRWQWDGVDFEFLMPSERKNIDDNGKSCVLRVVAGGAAL
                     LVTGDLDTKGEESLVGKYGGNLYSQVLVLGHHGSNTSSSGVFLNAVSPEYAVASSGYA
                     NAYKHPTEAVQNRVRAHGIKLLRTDLSGALQFGLGRGGVKAQRLRGYKFYWQKKPFE"
BASE COUNT         1357 a         1806 c         1372 g         1131 t
ORIGIN      
        1 ttctccgagc tttatatgtt tcaacagagg acggcacatc aagcaccgca ctgcgtgttg
       61 cccgaacgag actaccctcc gattagattc tatcgctata aacagacggg tttcaaccga
      121 aaaggaatgg ggataaagtc catttccgac acctctcggg cgatgccgtc tgaaaaccaa
      181 tctccacttt cagacggcat tgtttaggca cggccatttt gcaaattaag gttaaggcgt
      241 gtatgcttgc cttcccttaa aaacaaacac gcaacgcatt attatgaata tcctcagtaa
      301 aatcagcagc tttatcggaa aaacattttc cctctgggcc gcgctctttg ccgccgccgc
      361 ttttttcgcg cccgacacct tcaaatgggc ggggccttat attccttggc tgttgggcat
      421 tattatgttc ggtatgggtt tgacgctcaa accttccgac ttcgatattt tgttcaaaca
      481 tcccaaagcc gtcatcatcg gcgtaatcgc acaattcgcc attatgccgg caaccgcctg
      541 gctgctgtcc aaactgttga acctgcctgc cgaaatcgcg gtcggcgtga ttttggtcgg
      601 ctgctgcccg ggcggtacgg cttccaatgt gatgacctat ctggcgcgcg gcaatgtggc
      661 tttgtcggtt gccgttacgt ctgtttccac cctgatttcc ccattgctga ctcccgccat
      721 cttcctgatg cttgccggcg aaatgctgga aatccaagcg gccggtatgt tgatgtccat
      781 cgtcaaaatg gttttgctcc ccattgtttt gggtttgatt gtccataagg ttttgggcag
      841 taaaaccgaa aagctgaccg atgcgctgcc gctggtttcc gttgccgcca tcgtgctgat
      901 tatcggcgcg gttgttgggg caagcaaagg caagattatg gaaagcggcc tgctgatttt
      961 tgcggttgtc gtactccaca acggcatcgg ctacctgctc ggcttctttg ccgccaaatg
     1021 gaccggcctg ccttatgatg cacaaaaaac gctggccatc gaagtcggta tgcaaaactc
     1081 gggcctggcc gccgcgcttg ccgccgcaca ctttgccgct gcgccggtcg ttgccgttcc
     1141 gggcgcattg ttcagcgtgt ggcacaatat ctccggctcg ctgctggcaa cttattgggc
     1201 ggccaaagcc ggtaaacata aaaaacccta aacgcctttc atcataatgc cgtctgaacg
     1261 gggcaaacga aaccggcggt ttccgcccga ccccttcaga cggcctttgt ttgaaatcgg
     1321 cagggacgat tgtccggctg ctccgattcg gctgcctgcg ttcgccccgc ctttcaagcc
     1381 gcctactccc ttacataaac cacttcgaca tcgtagtcgt cttcgttcca atcgtcatcg
     1441 tcgtccgcgc cgaatttgtc ctgccattcc tcttcattgc tcaaagacga atccaaaccg
     1501 gcttcaagac ggaggacaga caacaaatga tatatgtcgt ccggaatcgg tgcctcaaac
     1561 gaaacggttt cgccgctttc cggatgggtg aaactcaagc ggtaggcgtg caacgcctga
     1621 cgcgcgccca aacttttaac ggcttctttc accgtgtcgc cgcacggatg gcgcgggttg
     1681 ccgtaaaccg ggtcggcggc aagcggatgg ttggcctcgc gcatatggac gcggatttgg
     1741 tgcgtcctgc ccgtttcgag cgagcattcg atatagctgt gggtaagata gcgttccaac
     1801 actttgacgt gggtaacggc tggtttgccg ccgaatttga cgactgccat tttcaggcgg
     1861 ttgtgcggat cgcgtccgat ttgggtttcg attttaccgt cgaaggggac gatgccgttg
     1921 gcgacggcgc ggtagatgcg tttgaccgtg cgctcttgaa gctgcctcac gagggaattt
     1981 tgcgccggca gggttttggc aaccaccatc agcccgctgg tttccttgtc caaacggtgt
     2041 acgatgcccg cgcgcggtac ttggctcaat tcgggacagt gcgccaacag gccgttgagc
     2101 agcgtccccg tccagttgcc cgccgccgga tgcaccacca gtccggccgg tttgttgacg
     2161 acgatgacgg tatcgtcttc gtaaacaata tccaaagcca taggctctgg aacaaacgcc
     2221 agattttcct cactcggacg gacggttaca caaatttgct cgccgcctat cattttgtct
     2281 ttgggttgcg aaggtttatc gtttacaata accgcgcctt ctttaatcca tgatgtcagg
     2341 cggctgcgcg agtagtcggg cagaagtttc gccaacaccg catccaaccg cccgccggca
     2401 agctcgagcg gaacggtcag accgatacgg ttttcagttt cggaagctga cgcaaagtct
     2461 gaatcgtcgc tataatcgga ttcattatca aaggaagtat tctgcatgaa aaaaattctt
     2521 ttaacggttt cattaggttt ggcactgagt gcctgtgcca ctcaaggtac ggtcgataaa
     2581 gatgctcaga ttacccaaga ttggagtgtg gagaagctct atgccgaagc ccaggacgaa
     2641 ttgaacagca gcaattatac gcgggctgtc aagttatacg aaatcttgga atcgcgcttc
     2701 cccaccagcc gccatgcccg gcaatcccaa ctggataccg catacgccta ttataaagac
     2761 gatgaaaaag acaaggctct ggcggcaatc gaacgcttcc gccgcctcca tccgcagcat
     2821 ccgaatatgg attacgcgct gtatctgcgc ggcttggtgc tgttcaacga agaccagtcc
     2881 ttcttgaaca aactggcctc gcaagactgg tccgaccgcg acccgaaagc caaccgcgaa
     2941 gcgtatcagg cctttgcgga actcgtccaa cgcttcccga acagcaaata cgccgccgat
     3001 gcaaccgcac gcatggtcaa actggtcgat gcactgggcg gcaatgaaat gtcggtggcg
     3061 cgttactaca tgaaacgcgg cgcatatatc gccgccgcca accgcgccca aaaaattatc
     3121 ggcagctatc aaaatacacg ctatgtcgaa gaatcgctcg ccatattgga acttgcctac
     3181 caaaaactcg gcaaaccgca gcttgccgcc gatacgcgcc gcgtgttgga aaccaacttc
     3241 ccgaaaagcc cgtttttgac gcacgcctgg cagcccgacg atatgccctg gtggcgttac
     3301 tggcattaag cccatcttcg taatgccgtc tgaagccgtt tcagacggca ttttatgttt
     3361 caaacctcat tcaaacggtt ttttctgcca atagaattta taccctctca aacgttgagc
     3421 cttcacgccg ccgcgtccca agccgaattg cagtgcgccc gacaaatcgg tacgcagcag
     3481 tttgatgccg tgtgcgcgga cacggttctg caccgcttcg gtcggatgtt tgtaggcgtt
     3541 ggcataaccg cttgaagcaa cggcatattc gggcgagacg gcattgagga acacgcccga
     3601 cgaggacgta ttgctgccgt gatgtcccaa aaccaaaacc tggctgtaca ggttgcctcc
     3661 atacttgccg accaggcttt cctcgccctt cgtatccaaa tcgcccgtta ccagcagtgc
     3721 cgcaccgccc gccacaacac gcaaaacaca acttttccca ttatcatcga tgtttttgcg
     3781 ttcagacggc atcaaaaact caaaatccac gccgtcccat tgccaacgct gttccgcaca
     3841 atgccgcgcc ccctcataaa attccggctg cccggcataa atcccgccgt tcggtatctt
     3901 gccgactgcc tgaaaaccgc cgtcgtggtc gctgtcgtga tgcgacaaaa ccaaataatc
     3961 caaatacctg attcccgacg cattcaagct cggcacaatc cccatctgcg ccgccgctgc
     4021 cgttccggtg tcaaacaaaa gatgatgatt tgccgtctga accgacaccg acaaaccctg
     4081 ccccgcatcc caaaccgtaa ccgcaacctc attttccggc acgccgggtg aacggtaaaa
     4141 cacaaaccct gccaacagca acaccgccca cggacgcaaa cccaagccgc gcggcagcag
     4201 caacagcaaa gcggcacaca ccaccaacac caacagcggc aaaggtgcgg aggcaacggc
     4261 aaactcgggc gacacatcgg caagccacac caaaaaccgc aaagtatatt ccgccaaaaa
     4321 tgcccccaac tgttgcaaag gcgcaaacgg cacgaccgaa cccagcaacg ccagcggcgt
     4381 caatacccaa gaaaaccacg gaattgccac cgcattgacc aaagggctga ccaaaggcag
     4441 cgaagcaaac agataaccga gcaaaaccag cgacaacacc gaagccgccc actgcccgcg
     4501 caaagcagtt tgccgttttc cctcgtgcaa acgccccgag cacgcccata tcagggccgc
     4561 caccaaaccg aaagacagcc aagtccccac acccaagacc gccgacgggt cgaacagcag
     4621 caccgccgcc aacgcctgcc accacgtcgc ccacgccgac aatcttcccc tgcgccaagc
     4681 ccacgcaaac gccgccaaca tcaaaacgct gcgctgcgtc ggcacggaaa aaccggcaag
     4741 cagcgcgtaa aacagcgcgc ctgcacaccc cgccgccaaa acccacgcgc gcggccgggc
     4801 aggaaggcgc ggggaacagg caagcagccg cttcgccagc cacgcaaaca ttaccgccac
     4861 catcgtaacg tgcaaacccg aaatactgac caaatgcgtc agccccaacg gccggaacgc
     4921 ctgccacaat tcggggcgca atgccgactg ttcgcccacg ctcaacgcgc gcatcagccc
     4981 gatgccgtct gaaagcccgc cgtccgcatc cgcctgctgc caattacggc tgatgcggct
     5041 gcgccaaacc gcaatccccc aaccgcttcc gccatgcagc aaaaccctgt ccgcaccgac
     5101 cgtccccgcg ccgcctattc cgttggataa tgcccacgcc tcacggttca aaccgcgcag
     5161 gttcaactcc ccgacgacag ggtgcacacg tgccgttatc cgccatctgc tcccgaccgc
     5221 ccattcgcgc cgtttgtagt ccgacagcag caaatcaaac gtccgaccac cgctgtccac
     5281 agcctttgcc gcaaactgca cacgccgccc gtccgacctc ggcatatccg ccacttccac
     5341 cgtcaaaggc acgcctgaaa cggcttctgc ccaccattgg gaagacaatg ccgcctccgt
     5401 cctgaatacg ccgtaagccg cgcccgccaa cacgcacagc atcagaccgg caaacgcaaa
     5461 acgccttgca aacacagcaa aaaccgcaaa agccgccagc caaaacagcc aatgcggcac
     5521 gaccggcagc gcgaacgaag ccgccgcacc gacaacccaa cacggcaaaa accaacgacg
     5581 gaacacgtct ccccacccca gatatttcaa aaataaacgg ataataaagc aaatccgaaa
     5641 aaccgtcata acaagatttg aaaatg
//
LOCUS       DAWHSK010000120         5647 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.120, whole genome shotgun sequence.
ACCESSION   DAWHSK010000120 DAWHSK010000000
VERSION     DAWHSK010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5647
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.120"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..290)
                     /locus_tag="WIC03_001356"
     CDS             complement(<1..290)
                     /locus_tag="WIC03_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ3883845.1"
                     /translation="MKITHCKLKKKVQKELLHFFVLEVTARSAADILGIHPNSAALFY
                     RKIRTVINYHLALAADEVFEGSVELDESYFGGQRKGRRGRGAAGKVVVFGIL"
     gene            complement(448..2454)
                     /locus_tag="WIC03_001357"
     CDS             complement(448..2454)
                     /locus_tag="WIC03_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HEZ3883846.1"
                     /translation="MADMKKITPQNLRPLLSESLGHTDFVNVLNALIKFLRRGGKKCA
                     GERFDLIIDTFKQDRELLSRFSRCFYIWLAQIHIYPALIKLGIFSRHSFAREMGIRIY
                     ERFSPSYKDFANLGEVFLYLFHSENDDKWLQTLNIRQWLVLYELIRSHAEPSKLQTAG
                     IRLADARLRAIEMLSVWTASEAIEPDLIRIAPRLLEADSSFVALQRETAKLVEHYRNG
                     TAPYDTAHLEVMFDQCFSQIDYLRRKGTGAGSGSSVKVAHLLERLRQTVGRLKLLTDI
                     QTGVGNSNRLTIALMNSLIYAAVEQYSTRHLRRSSIRMLARSITENKSHHGEHYITRN
                     RKEYFKMFYSAAGGGIIIALMALLKIRIGSLGLSPFLTSLSAGFNYGIGFMIIHMLHC
                     TVATKQPAMTAASFAEQVDLNEGGKAVDNKLAKLLIDVCRSQSVAVFGNVSIAILLAC
                     AISFGYAHLYRLPILDAHTAAYQFKSIDIIAYPTLWYAAIAGLWLFCSGIIAGFFDNR
                     ADYLNLRQRLPFNPLLRKIMRPGPRRVLAAYIHKHYGSLVGNFIFGMLLGMTGYFGHL
                     LGLPLDIRHVAFSSANLGYAAVSGNVGLGTFVLGIFSVLTIGLVNLCVSFSLALFVAL
                     RSRGTKIGSIRNLIKSFWNQIKSNPCILFLPPAKEQGHPPSDKP"
     gene            2743..4770
                     /locus_tag="WIC03_001358"
     CDS             2743..4770
                     /locus_tag="WIC03_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HEZ3883847.1"
                     /translation="MSLSEFIERRTSFNPMVILTTLFFVCVLAVLVLTVPDQVQMWLD
                     RAKEVIFTEFGWFYVLTFSIFLGFLLILSVSSLGNIRLGRDEDVPEFGFLSWLAMLFA
                     AGMGVGLMFFGVAEPLMHYFSDITAGTPEHRQQQALLHTVFHWGVHAWSVYGTIALAL
                     AYFGFRYKLPLALRSCFYPLLKEKISGRFGDAIDIMALLATFFGIITTLGFGASQLGA
                     GLQEMGWIAENSFSVQVLIIAAVMSLAVVSAISGVGKGVKVLSELNLSLAFLLLFFVL
                     AAGPTVYLLSAFGDNIGNYLENLVRLSFKTYAYEREHKPWFESWTVLYWAWWCSWAPF
                     VGLFIARISKGRTIREFVFGVLLIPGLFGVLWFTVFGNTAIWLNDGVAGGMLEKMTSS
                     PETLLFKFFNYLPLPELTSIVSLLVISLFFVTSADSGIYVLNNITSRDKGLSAPRWQA
                     VMWGVLMSAVAVLLMRSGGLGNLQSMTLIVSLPFALLMLIMCFSLWKGLSADKKYFET
                     RVNPTSVFWTGGKWKERLVQIMSQTQEQDILKFLKHTASPAMHELQRELSEEYGLSVR
                     VDKMFHQDEPAIEFVIRKETMRDFMYGIKSVGQDVSDQLINDGKLPHIRHQTTYKPYA
                     YFFDGRVGYDVQYMNKDELIADILKNYERYLMLLDDVGQELMAHEQVELAE"
     gene            complement(4862..>5647)
                     /locus_tag="WIC03_001359"
     CDS             complement(4862..>5647)
                     /locus_tag="WIC03_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEZ3883848.1"
                     /translation="RATVFLGIPAIYTAMSKAKIPWYFRWFNRIRLFISGGAPLAEQT
                     ILDFKAKFPRAKLLEGYGLSEASPVVAVNTPERQKARSVGIPLPGLEAKAVDEELVEV
                     PRGEVGELIVRGGSVMRGYLNMPAATDETIVNGWLKTGDFVTIDEDGFIFIVDRKKDL
                     IISKGQNVYPREIEEEIYKLDAVEAAAVIGVKDRYADEEIVAFVQLKEGMDLGENEIR
                     RHLRTVLANFKIPKQIHFKDGLPRNATGKVLKRVLKEQFDGNK"
BASE COUNT         1182 a         1313 c         1696 g         1456 t
ORIGIN      
        1 ttcagaatgc cgaaaacaac cacttttcct gccgcaccgc gaccacgtct gcctttacgc
       61 tgtccgccga aatagctttc gtccagctcg acagaaccct caaaaacctc atcggcagcc
      121 aaggccaaat gatagttgat aaccgtgcgg attttacggt agaacagtgc tgccgaattg
      181 ggatggatac ccaaaatatc ggcggcagaa cgggcggtaa cttccagtac aaaaaaatgg
      241 agcagttctt tctgtacttt tttctttaat ttgcagtgcg ttatcttcat atttcgaggg
      301 taacatatct gctaatctag tacagcccct tttaaaatgc cgtctgaacc acgtttcggt
      361 tcagacggca tttttgcatc cttttatggg gattttccgc atcagggcgg tattcgggga
      421 gaagcccgct tcagacggca ttgccggtca aggcttgtcc gaaggaggat gtccctgttc
      481 tttggcgggc gggaggaaaa gtatgcaggg attgctttta atctgattcc aaaaactctt
      541 aatcagattg cggatgctgc cgattttcgt gccgcgcgag cgcagggcga cgaagagggc
      601 gaggctgaaa ctgacgcaga ggttgaccag gccgatggtg aggacgctga aaatgccgag
      661 tacgaacgtg cccaaaccga cgttgccgct gacggcggca tagccgaggt tggcggagga
      721 aaaggcgacg tggcggatgt ccagcggcag cccgaggagg tgtccgaaat agccggtcat
      781 acccaagagc atcccgaaga tgaagttgcc taccaacgag ccgtagtgtt tgtggatgta
      841 ggcggcgagg acgcggcggg gcccggggcg catgatttta cgcagcaagg ggttgaaggg
      901 caggcgttgg cgcaggttga ggtagtcggc gcggttgtcg aaaaaacctg cgatgatgcc
      961 ggagcagaac agccacagac ctgcaatggc ggcataccac agcgtcgggt aagcgatgat
     1021 gtctatggat ttgaactggt aggcggcggt gtgggcatcg agtatgggca gccggtacag
     1081 atgggcatag ccgaacgata tggcgcacgc caaaaggatg gcgatggaaa cgttgccgaa
     1141 gacggcgaca ctttgggagc ggcatacgtc gatgaggagc ttggcgagtt tgttgtccac
     1201 cgctttgccg ccttcgttga gatcgacctg ttcggcaaag ctggcggcag tcatcgcggg
     1261 ctgcttggtg gcgacggtgc agtgcagcat atggatgatc ataaagccga tgccgtagtt
     1321 gaacccagcc gacaaggaag tgaggaaggg gctgaggccg agtgagccga tgcggatttt
     1381 gagcagcgcc atcagggcga tgatgatgcc gccgcctgcc gccgagtaga acattttgaa
     1441 atattctttg cggttgcggg tgatgtagtg ttcgccgtgg tggcttttgt tttcggtaat
     1501 gctgcgggcg agcatacgga tgctgctgcg gcgcaggtgg cgggtgctgt attgttcgac
     1561 tgccgcgtag atgagggagt tcatcagcgc gatggtcagg cggttgctgt tgccaacacc
     1621 ggtttggatg tcggtaagca gcttcagacg gcctacggtt tgccggagcc gttcgagcag
     1681 gtgggcgact ttgaccgacg aaccggagcc tgcgcccgtc cctttgcggc gcaaatagtc
     1741 aatctggctg aaacattggt cgaacatcac ttcgaggtgg gcggtgtcgt aaggcgcggt
     1801 gccgttgcgg tagtgttcga ccagtttggc ggtttcgcgt tggagggcga cgaaggaaga
     1861 atcggcctcc agcaggcgcg gggcgatgcg gatgaggtcg ggttcgatgg cttcggatgc
     1921 cgtccagaca gacagcattt cgatggcgcg caaacgcgca tcggcaaggc ggatgcccgc
     1981 cgtctgcaat ttggacggct cggcgtggct ccggatgagt tcgtataaaa ccagccattg
     2041 gcggatattg agcgtttgca gccatttgtc gtcgttttcg gaatggaaaa gataaaggaa
     2101 gacttcgccc aagttggcaa aatctttata tgacgggctg aagcgttcgt agatgcgtat
     2161 gcccatttcc cgggcaaagc tgtggcgcga gaagatgccg agtttgatga gtgccggata
     2221 aatgtgtatt tgcgcgagcc agatgtaaaa acaccggctg aagcgggaca gtaattccct
     2281 gtcttgtttg aatgtgtcga taatcaggtc gaaacgttcc cccgcacatt ttttgccgcc
     2341 acggcgcaaa aatttaatca gtgcgttgag gacgttgaca aaatcggtat gtcccaagct
     2401 ttccgaaagc agggggcgca ggttttgagg ggtaattttc ttcatatcag ccattgtacc
     2461 gcatcggcaa tccggcgtta aacgccccga tagcggggag ggcgcgcctg cggtgggcgg
     2521 cgcattttcc gccggtttga aatcaaacta tattaatgat ataataaata tcgataaatc
     2581 aatctgatag aataatccgt attgattttt aattttttcg attataacaa tttcggccgg
     2641 tttgtgcatt tcgtcatccg gaagccggta ttttaccggc aggttttgac cgctttgccc
     2701 gatgaccggg tttgtctgtt tttaacaaag gagtgagaag gtttgtccct gtctgaattt
     2761 atagaacgcc gaacgtcatt taatccgatg gttattttga cgactttgtt ttttgtgtgt
     2821 gttttggcgg tattggtttt aaccgtgccg gatcaggtgc agatgtggct cgatcgggca
     2881 aaagaagtca tttttaccga gttcggctgg ttttatgttt taacgttttc catttttctg
     2941 ggtttcctgc tgatactctc ggtcagcagt ttgggaaaca tcaggctcgg acgggatgaa
     3001 gatgtgccgg aattcggctt cctgtcgtgg ctggcgatgc tgtttgcggc cgggatgggc
     3061 gtgggtctga tgtttttcgg cgtggcagag ccgttgatgc attatttttc ggacattacg
     3121 gccggcacgc cggaacacag gcagcagcag gcattgctgc acacggtgtt ccattggggc
     3181 gttcacgctt ggtcggtgta cggtacgatt gcattggctt tggcttattt cggtttccgc
     3241 tacaagctgc cgcttgccct gcgttcttgt ttttaccccc tgttgaaaga aaaaatttcc
     3301 ggaaggttcg gcgatgccat tgatattatg gcgttgcttg ctactttttt cggcatcatc
     3361 accacattgg ggttcggggc ttcgcaactg ggcgccggat tgcaggaaat gggctggatt
     3421 gccgaaaaca gcttcagcgt gcaggttttg attatcgccg ccgtcatgtc cctcgccgtc
     3481 gtttcggcaa tatccggcgt ggggaagggc gtgaaggtgt tgagcgagtt gaacctgagc
     3541 cttgcgtttt tgctgctgtt ttttgttttg gcggcgggcc ccactgttta cctgttgtcg
     3601 gcattcggcg acaacatagg gaactacctc gaaaatctgg tgcgcctcag ttttaaaact
     3661 tatgcgtacg aacgggaaca caagccgtgg tttgaatctt ggacggtgct ttattgggcg
     3721 tggtggtgtt cttgggcgcc gtttgtgggt ttgtttatcg cgcgtatttc aaaggggcgc
     3781 accatccgcg agtttgtctt cggggttttg ctcatccccg gcctgttcgg cgttttgtgg
     3841 tttaccgtct tcggcaatac ggcgatttgg ctgaatgacg gggttgcggg gggaatgctc
     3901 gaaaagatga cctcctctcc ggaaacgctg ctttttaaat tctttaatta cctccccctg
     3961 cccgaattga cgagcatcgt cagcctgctg gtcatttccc tgttttttgt aacttctgcc
     4021 gattccggga tttatgtcct gaacaatatt acctctcggg acaagggctt gagcgcacca
     4081 cggtggcagg cggttatgtg gggcgtgctg atgtctgccg ttgccgtttt gctgatgcgc
     4141 tcgggcgggc taggcaacct gcagtctatg accctgattg tttccctgcc gtttgccctg
     4201 ctgatgctga taatgtgttt cagcctgtgg aaaggattga gtgcggataa gaaatatttt
     4261 gagacccggg ttaaccctac cagtgtattt tggacgggcg gcaagtggaa agaacggctg
     4321 gtgcagataa tgagccagac gcaggagcag gatattttaa aattcctcaa acataccgca
     4381 tcgcccgcta tgcacgagtt gcaacgggag ctttcggaag aatacggctt gagcgtccgg
     4441 gtcgataaga tgtttcatca ggacgagccc gcaatcgagt tcgtcattcg gaaagagacg
     4501 atgcgcgatt ttatgtacgg gattaagtct gtcgggcagg atgtatccga ccagttgatt
     4561 aacgacggca agctgccgca tatccggcat cagacaactt acaaacccta cgcttatttt
     4621 ttcgacgggc gcgtcgggta cgatgtgcag tatatgaaca aggacgagct gattgccgac
     4681 attttgaaaa actacgaacg ttatttgatg ttgttggatg atgtcggtca ggaactgatg
     4741 gcgcacgagc aggtggaatt ggcagagtaa gtgccttatc tccggtgttt gccaaaaaaa
     4801 atgcggtgaa tcagattcac cgcatttttt ttgccggacg gggcttcgga cggcacggcg
     4861 ttcatttgtt tccgtcaaac tgctccttca acacccgttt caataccttg cccgtagcgt
     4921 tgcgcggcag cccgtcttta aagtggattt gtttggggat tttgaaattt gccagcacgg
     4981 tacgcaggtg gcggcggatt tcgttctcgc ccaaatccat accttccttc aattggacga
     5041 aggcgacgat ttcctcgtcg gcataacggt ctttcacgcc gatgacggcg gcggcttcga
     5101 cggcatcgag tttgtagatt tcttcttcga tttcgcgcgg atagacgttt tgacctttgg
     5161 aaataatcaa atcttttttg cggtcgacga taaagataaa gccgtcttcg tctatggtaa
     5221 cgaaatcgcc cgttttcaac cagccgttga cgatggtttc atcggtggcg gcaggcatat
     5281 tgaggtagcc ccgcatcacc gaaccgcccc tgacgatcag ttcgcccact tcgccgcgcg
     5341 gcacttcgac caattcttca tcgacggctt tggcttccaa accgggcagg gggatgccga
     5401 cgctgcgggc tttttgcctc tcgggcgtat tgacggcgac gacgggagag gcttcgctca
     5461 gtccgtagcc ttccagcaat ttggcgcggg ggaacttggc tttgaaatcg aggatggttt
     5521 gttccgccaa aggcgcaccg ccgctgataa acaggcgaat gcggttgaac catctgaaat
     5581 accaagggat tttcgccttg ctcattgcgg tgtaaatcgc gggtatgccc aaaaacacgg
     5641 tcgcgcg
//
LOCUS       DAWHSK010000121         5514 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.121, whole genome shotgun sequence.
ACCESSION   DAWHSK010000121 DAWHSK010000000
VERSION     DAWHSK010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5514
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.121"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(609..3080)
                     /locus_tag="WIC03_001360"
     CDS             complement(609..3080)
                     /locus_tag="WIC03_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEZ3883849.1"
                     /translation="MKKTCFHCGLDVPEHLHLTVRYENEDRETCCAGCQAVAQSIIDA
                     GLGSYYKQRTADAQKTELPPQEILDQIRLYDLPEVQSDFVETHGGTREAVLMLGGITC
                     AACVWLIEQQLLRTDGIVRIDLNYSTHRCRVVWDDGKIRLSDILLKIRQIGYTAAPYD
                     AQKIEAANQKERKQYIVRLAVAGLGMMQTMMFALPTYLYGGDIEPDFLQILHWGGFLM
                     VLPVVFYCAVPFYQGALRDLKNRRVGMDTPITVAIIMTFIAGVYSLATNAGQGMYFES
                     IAMLLFFLLGGRFMEHIARRKAGDAAERLVKLIPAFCHHMPDYPDTQETCEAAVVKLK
                     AGDIVLVKPGETIPVDGTVLEGSSAVNESMLTGESLPVAKMPSEKVTAGTLNTQSPLI
                     IRTDRTGGGTRLSHIVRLLDRALAQKPRTAELAEQYASSFIFGELLLAVPVFIGWTLY
                     ADAHTALWITVALLVITCPCALSLATPTALAASTGTLAREGILIGGKQAIETLAQTTD
                     IIFDKTGTLTQGKPAVRRISLLRGTDEAFVLAVAQALEQQSEHPLARAILNCRISDGS
                     VPDIAIKQRLNRIGEGVGAQLTVNGETQVWALGRASYVAEISGKEPQTEGGGSAVYFG
                     SQSGFQAVFYLTDPLKDSAAEAVRQLAGKNLTLHILSGDRETAVAETARALGVAHYRA
                     QAMPEDKLEYVKALQKEGKKVLMIGDGINDAPVLAQADVSAAAAGGTDIARDGADIVL
                     LNEDLRTVAHLLDQARRTRHIIRQNLIWAGAYNIIAVPLAVLGYVQPWIAALGMSFSS
                     LAVLGNALRLHKRGKMQSEKMPSEQ"
     gene            complement(3077..4255)
                     /gene="hflX"
                     /locus_tag="WIC03_001361"
     CDS             complement(3077..4255)
                     /gene="hflX"
                     /locus_tag="WIC03_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEZ3883850.1"
                     /translation="MTGRTGRNGSTQAQPERVMLVGVMLDKDGTGSSATRLNGFQTAL
                     AEAVELVKAAGGDSVRVETAKRDRPHTALFVGTGKAAELSEAVAADGIDLVVFNHELT
                     PTQERNLEKILQCRVLDRVGLILAIFARRARTQEGRLQVELAQLSHLAGRLIRGYGHL
                     QSQRGGIGMKGPGETKLETDRRLIAHRINALKKQLANLKKQRALRRKSRESGTIKTFA
                     LVGYTNVGKSSLFNRLTKSGIYAKDQLFATLDTTARRLYISPECSIILTDTVGFVSDL
                     PHKLISAFSATLEETAQADVLLHVVDAAAPNSGQQIEDVENVLQEIHAGDIPCIKVYN
                     KTDLLPSEEQNTGIWRDAAGKIAAVRISVAENTGIDALREAIAESCAAAPNTDETEMP
                     "
     gene            complement(4260..5405)
                     /locus_tag="WIC03_001362"
     CDS             complement(4260..5405)
                     /locus_tag="WIC03_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEZ3883851.1"
                     /translation="MHYLNRIRAQIGLHKLAHAPVLENSARRHARYLTLNPEDGHGEH
                     HPDNPHYTAQKLTERTRLAGYLYNGVHENISTEEEAAESSDSDIRTQQRQVDGLMSAI
                     YHRLSLLDRHTDEAGAAFVRENGKTVLVFNQGNGRFERHCAQGRNQPEAGRKYYRNAC
                     HNGAVVYTDEAMPAQELLYTAYPVGNGALPYFHGERPDPVPEYEITGNPASIDFSEAA
                     GKITMKSFKLYQGKNEIRPVRVLTAGNDPNGRLTAYQFALFPLKPLEYGTLYTAVFDY
                     VRNGRRAQAKWQFRTRKPDYPYFEVNGGETLAVRKGEKYFIHWRGRWCLEACTRYTYR
                     QRPGSRLSIGRHEAGGIVFSVDGMAGSRITLAPEGETERGVTLYLQD"
BASE COUNT         1102 a         1458 c         1599 g         1355 t
ORIGIN      
        1 ctgtaccggt ttaaatttaa ttcactatat agtgggtaaa aattagaaat gcacatttcc
       61 gtcattcacg cgcaggcggg aatccagacc tatccgttaa ttagcatttg aaaataaaag
      121 aaaaaccaag catatggatt cccccctacg cgagactgac gacggtgttc tgcagtagcc
      181 aaagcagaaa accatcctta ttgtcatttc atcatgatat aacaataagg atgttaaaaa
      241 ttagttatca ttatatgcat gatatataag aaaaattgtc ccaataagaa atgttatgca
      301 gaaaataaaa tgaaaaggat ggttttcatt gaaaatggtt gttctgccac cgtcatacac
      361 agaaccggta tttggaaaaa aaataatcca tattgacatc attattccat ttatcgcata
      421 aatccacttt ttaatttttt tcctcattaa tacaatctct tatttaataa aatcaaatac
      481 ctagcaattc gatctcaaat tttgaacgcc aacgtgtttg agcggtaaca atacatactt
      541 taagtttgca attcttcatc gtcttatttt tgttttcatc aactatacgt ctaaagcaac
      601 gccgtccgtc attgttcgga cggcattttt tcagactgca ttttcccccg tttgtgaagg
      661 cgcagggcgt tgcccaaaac cgccagcgaa ctgaagctca tacccagtgc ggctatccac
      721 ggttggacat agcccaaaac ggcaagcggt acggcaatga tattgtacgc gcccgcccat
      781 atcaggtttt gccggataat atggcgggtg cgccgcgcct gatcgagcag gtgggcgacg
      841 gtacgcaaat cttcgttcaa taacacaatg tccgcgccgt ccctcgcaat atccgtcccg
      901 cccgctgcgg cggcggatac gtctgcctgc gccaaaacgg gcgcgtcgtt gatgccgtcg
      961 cctatcatca gcactttttt cccttctttt tgcaaggctt tgacgtattc cagtttgtcc
     1021 tcgggcatgg cttgggcgcg gtagtgcgcg acacccaggg cgcgtgcggt ttcggcaacg
     1081 gcggtttcgc ggtcgccgct gaggatgtgc agggtcaggt ttttgcctgc caactgccgc
     1141 accgcctccg ccgcgctgtc tttcaagggg tcggtcaggt agaacacggc ttggaaaccg
     1201 ctttgactgc cgaagtaaac cgcgctgccg ccgccttctg tttgcggttc tttacctgaa
     1261 atttcggcga cataggatgc cctgcccaat gcccaaacct gtgtttcgcc gttgacggtc
     1321 agttgcgcgc ccacgccttc gccgatgcgg ttgaggcgtt gtttaatagc gatgtcgggg
     1381 acgctgccgt ctgaaatgcg gcagttgagg atggcgcggg caaggggatg ttcggactgt
     1441 tgttctaaag cctgcgccac cgcgagaaca aaggcttcgt ctgtgcctct caacaatgag
     1501 atacggcgga cggcgggttt gccttgggtc agcgtgccgg ttttgtcgaa gatgatgtcg
     1561 gtggtttggg cgagggtttc gattgcctgc tttccgccga ttaaaatacc ttcgcgcgcc
     1621 agcgtaccgg tagaagctgc cagcgcggtc ggcgtggcaa gcgataaggc gcaggggcag
     1681 gtaatgacca gcagggcgac ggtaatccac aatgcggtgt gcgcgtcggc gtacagcgtc
     1741 cagccgatga agacggggac ggcaagcagg agttcgccga atatgaaaga cgaggcgtat
     1801 tgttccgcca actcggcagt gcgcggtttt tgcgctaagg cgcggtcgag caggcggacg
     1861 atgtgcgaca gtcgcgtgcc accgccggtg cggtcggtgc gtataatcag ggggctttgc
     1921 gtgttgagtg tgccggcggt tactttttca gacggcattt tggcgacggg caggctctcg
     1981 ccggtcagca tagattcgtt gacggcactg cttccttcca gcaccgtgcc gtcaacgggg
     2041 atggtttcgc ccggtttgac cagcacgata tcgcccgcct tcaatttgac gacagctgcc
     2101 tcgcaggttt cctgcgtatc ggggtaatcg ggcatatgat ggcaaaacgc aggaatcagc
     2161 ttcaccagcc tctcggcggc atcgcctgcc ttacggcggg caatgtgttc cataaagcgt
     2221 ccgcccagca ggaaaaacag cagcatcgcg atggattcga aatacatccc ctgccccgca
     2281 tttgtcgcaa ggctgtaaac gccggcgata aaggtcatga tgatggcgac ggtaatcggc
     2341 gtatccatgc cgacgcggcg gtttttcaag tcgcgcagcg cgccttgata aaacgggacg
     2401 gcgcaataga atacgacggg cagcaccatt aaaaagccgc cccaatggag gatttgcagg
     2461 aaatcgggtt cgatgtcgcc gccgtaaagg taggtcggca gcgcgaacat catcgtctgc
     2521 atcatcccca gcccggcaac ggcgaggcgg acgatgtatt gtttgcgttc tttttggttg
     2581 gcggcttcga ttttttgcgc gtcatagggt gcggcggtgt agcctatctg cctgattttc
     2641 aacagaatgt cggaaaggcg gattttgccg tcgtcccaga cgacgcggca gcggtgcgtg
     2701 ctgtaattga ggtcgatgcg gacgatgccg tctgtacgca aaagctgctg ttcgatcagc
     2761 cagacgcagg cggcgcaggt gatgccgccg agcattaaaa ccgcctcgcg cgtgccgccg
     2821 tgggtttcca caaagtcgga ctggacttcg ggcaggtcgt acaggcgaat ttggtcgagg
     2881 atttcttggg gcggcagctc ggttttttgc gcgtcggcgg tgcgttgttt gtaataactg
     2941 cccaagcccg cgtcaataat gctttgtgcg accgcctgac agccggcgca gcaggtttcg
     3001 cggtcttcgt tttcgtaacg gacagtcagg tggaggtgtt cgggaacatc cagaccgcag
     3061 tggaaacagg tttttttcat ggcatttcgg tttcgtctgt gtttggtgcg gcggcacaag
     3121 actcggcaat ggcttcgcgc agtgcgtcta taccggtatt ttcagcaacg gaaatgcgga
     3181 cggcggcaat ttttcccgca gcgtcgcgcc atatgcccgt gttttgttct tcagacggca
     3241 gcaggtcggt tttgttgtac accttgatgc acggaatatc gccggcatgg atttcttgca
     3301 gtacgttttc cacgtcttca atctgctgtc cgctgttcgg agcggcggca tcgacgacgt
     3361 gcagcagcac atcggcttgc gcggtttctt ccagcgtggc ggaaaaggcg gaaatcagtt
     3421 tgtgcggcag atcgctgacg aatccgacgg tatcggtcag gataatgctg cattcgggac
     3481 tgatgtacag ccgccgcgcc gtcgtgtcga gtgtggcgaa aagctggtct ttcgcatata
     3541 tgcccgactt ggtcagccgg ttgaacaggc tggatttgcc gacattggta tagccgacca
     3601 gcgcaaacgt tttgattgtg cccgattcgc gggacttgcg gcgcagggcg cgctgttttt
     3661 tgaggttggc aagctgtttt ttcaaggcgt tgattcgatg ggcgatcaat cggcggtcgg
     3721 tttccagttt ggtttcgccg gggcctttca tgccgatacc accgcgctgg ctctgcagat
     3781 ggccgtaacc gcgtatcaag cgtcccgcca aatggctcaa ttgcgccaac tcgacttgca
     3841 gcctgccttc ctgcgtgcgg gcgcggcggg cgaaaatcgc caaaatcaac cctaccctgt
     3901 ccaatacgcg gcattggagg attttttcca aattgcgttc ctgcgtgggc gtaagttcgt
     3961 ggttgaatac gaccaaatcg atgccgtctg cggcaactgc ttctgacagc tccgccgcct
     4021 tgcccgtgcc gacaaacagc gcggtgtgcg gacggtcgcg tttggcagtc tccacgcgca
     4081 cggaatcgcc gcccgccgct ttgaccagct cgacagcttc cgccaatgcc gtctgaaaac
     4141 cgttcagacg ggtggcactg ctgcccgtac catctttgtc caacattacg cccaccagca
     4201 tgacgcgttc gggttgcgct tgggtactgc cgttgcggcc tgttctgcct gtcatgtatt
     4261 caatcctgta aataaagggt tacgcctcgt tccgtttctc cttccggtgc aagcgtgatg
     4321 cggctgcccg ccattccgtc aacgctgaag acgatgccgc ccgcctcgtg ccttcctatg
     4381 gacaggcggc tgccgggtcg ctgccggtag gtataacggg tacacgcttc caaacaccag
     4441 cgtccgcgcc agtggatgaa atatttttcg ccttttctaa ccgcaagtgt ctcgccgccg
     4501 tttacctcaa aataagggta atcgggtttt cgggttctaa actgccattt cgcctgcgcg
     4561 cgccgtccgt tgcggacata gtcgaatacc gccgtataaa gcgtaccgta ttccaaaggc
     4621 ttgagcggga aaagcgcgaa ttggtacgcg gtcagcctgc cgttggggtc gttgccggcg
     4681 gttaaaaccc tgacggggcg gatttcgttt ttaccctgat acagcttgaa acttttcatc
     4741 gtaattttgc ctgccgcctc ggaaaaatca atgctggcag gattgcccgt gatttcatat
     4801 tccggcacgg ggtctggacg ctcgccgtgg aaataaggca gtgcgccgtt gccgacggga
     4861 taggctgtat agagcagctc ctgtgcgggc atggcttcgt cggtgtacac gaccgcaccg
     4921 ttatggcagg cgttgcggta atatttccgt cctgcttccg gctgatttct gccttgggcg
     4981 caatgccgct caaacctgcc gttgccctga ttgaatacga gaacggtttt accgttttcg
     5041 cgcacaaatg ccgctcctgc ctcatccgta tggcggtcaa gtagggaaag gcggtggtag
     5101 attgcgctca ttaatccgtc cacttggcgt tgctgcgtgc ggatgtcgct gtcggacgat
     5161 tcggcggctt cctcttccgt gctgatgttt tcatgcacgc cgttgtagag atacccggca
     5221 aggcgtgtgc gttcggtcag cttttgtgcg gtgtagtgcg gattgtcggg atggtgttcg
     5281 ccgtgtccgt cttcgggatt gagcgtgaga tagcgtgcgt gcctgcgggc ggaattttcc
     5341 aaaaccggcg cgtgtgccag cttgtgcaaa ccgatttggg cgcggatgcg gttgaggtag
     5401 tgcagcgcat cgccgccgtc cctgatttgc ggtgcggacg gatagacaag ttcgcccgcg
     5461 ggcagggatt ggttttgggt atgatagaaa acgcctgccg ccgaacccaa cagg
//
LOCUS       DAWHSK010000122         5473 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.122, whole genome shotgun sequence.
ACCESSION   DAWHSK010000122 DAWHSK010000000
VERSION     DAWHSK010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5473
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.122"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(253..1512)
                     /gene="rho"
                     /locus_tag="WIC03_001363"
     CDS             complement(253..1512)
                     /gene="rho"
                     /locus_tag="WIC03_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEZ3883852.1"
                     /translation="MHVSELQTLHISKLLELAEEHGIENANRFRKQDLVFAIVRQMMK
                     KGEGFTCSGTLEILPDGFGFLRSADTSYLAGPDDIYVSPTQIRRFNLHTGDTIEGSVR
                     VPKDNERYFALVRLDTINGDHPEVCRHKILFENLTPLFPTEQLKLERDLKSEENLTGR
                     AIDLISPIGKGQRALLVAPPKSGKTVMLQNIAHAVTANYPEVELIVLLIDERPEEVTE
                     MSRSVRGEVVSSTFDEPAQRHVQVAEMVLEKAKRMVEHKKDVVILLDSITRLARAYNT
                     VVPTSGKILTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALVETGSRMDDVIYEE
                     FKGTGNMELHLDRRMAEKRLFPAININKSGTRREELLVPNDQLQRMWLLRKFLHPMDE
                     IEAAEFLIGKIKASKNNDDFFELMRGK"
     gene            complement(1732..4116)
                     /gene="ppsA"
                     /locus_tag="WIC03_001364"
     CDS             complement(1732..4116)
                     /gene="ppsA"
                     /locus_tag="WIC03_001364"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HEZ3883853.1"
                     /translation="MADNYVIWFENLRMTDVERVGGKNASLGEMISQLTEKGVRVPGG
                     FATTAEAYRAFLAHNGLSERISAALAKLDVEDVAELARVGKEIRQWILDTPFPEQLDA
                     EIEAAWNKMVADAGGADISVAVRSSATAEDLPDASFAGQQETFLNINGLENVKEAMHH
                     VFASLYNDRAISYRVHKGFEHDIVALSAGVQRMVRSDSGASGVMFTLDTESGYDQVVF
                     VTSSYGLGENVVQGAVNPDEFYVFKPTLKAGKPAILRKTMGSKHIKMIFTDKAEAGKS
                     VTNVDVPEEDRNRFSITDEEITELAHYALTIEKHYGRPMDIEWGRDGLDGKLYILQAR
                     PETVKSQEEGSRNLRRFAINGDKTVLCEGRAIGQKVGQGKVRLIKDASEMDSVEAGDV
                     LVTDMTDPDWEPVMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGNATELLENGQE
                     VTVSCAEGDTGFIYAGLLDVQITDVALDNMPKAPVKVMMNVGNPELAFSFANLPSEGI
                     GLARMEFIINRQIGIHPKALLEFDKQDDELKAEITRRIAGYASPVDFYVDKIAEGVAT
                     LAASVYPRKTIVRMSDFKSNEYANLVGGNVYEPHEENPMLGFRGAARYVADNFKDCFA
                     LECKALKRVRDEMGLTNVEIMIPFVRTLGEAEAVVKALKENGLERGKNGLRLIMMCEL
                     PSNAVLAEQFLQYFDGFSIGSNDMTQLTLGLDRDSGLVSESFDERNPAVKVMLHLAIS
                     ACRKQNKYVGICGQGPSDHPDFAKWLVEEGIESVSLNPDTVIETWLYLANELNK"
     gene            4527..5348
                     /locus_tag="WIC03_001365"
     CDS             4527..5348
                     /locus_tag="WIC03_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HEZ3883854.1"
                     /translation="MSSPRHVFYISDRTGLTAENIGEALLNQFGNLSFKRHTHPFVDT
                     PEKARAVVEKVNRSRQENGQRPIAFVSVVDDEIRRIIKGADAFQINFFETFLGLLEKE
                     LNTEATASEQGHHSIGNTKRYDARMEAVNFSLNHDDGVSDKNLQEADVILMGVSRSGK
                     TPTCLYLALQYGIRAANYPLIPDDLESADLPRMVKPYRDKLFGLTIQPERLQAIRQER
                     RPNSTYAKIDTCRSEVADAQSMFRRHGIPFANTTDKSVEELAVHILQACKLKRRF"
BASE COUNT         1154 a         1284 c         1611 g         1424 t
ORIGIN      
        1 aatttataca aaaatgagcg cggttcggcg caaccttgaa tcaagttccc gcatcggttt
       61 tcattgccgg tacggatgcg ttcaagccgg ttttgcaaag gccgcgcttt cggcaagcgg
      121 acacggacac tgccggcggt tgcgccgtta acggggggag ggaggagctg cgccggccgt
      181 gtgaatgaaa gtgccgtctg aaacccgatt ttcaggtttc agacggcatt tcgcctaatg
      241 tcgatgatgt gtttattttc cgcgcatcag ttcaaagaaa tcatcattgt ttttagaggc
      301 tttgattttt ccgattaaaa attcggctgc ctcgatttcg tccatcgggt gcaggaactt
      361 gcgcagcagc cacatacgtt gcagctggtc gttcggcaca agcagctcct cgcggcgcgt
      421 gccggatttg ttgatgttga tggcggggaa gagacgtttt tccgccatac ggcggtcaag
      481 gtgcaattcc atattgccgg tgcctttgaa ttcttcgtaa atcacatcgt ccatacggct
      541 gccggtttca accaatgctg tggcgatgat ggtcagcgaa ccgccttctt ccacgttgcg
      601 cgccgcgccg aagaaacgtt tggggcggtg cagcgcgttg gcatcgacac cgccggtcag
      661 gattttgccc gaggtaggca cgacggtatt gtaggcgcgg gcaaggcggg taatcgaatc
      721 aagcaggatg accacatctt ttttgtgttc taccatacgc ttggcttttt caagcaccat
      781 ttcggcaact tggacgtggc gttgagccgg ctcgtcaaag gtggaggaga ctacttcgcc
      841 tcggacggaa cggctcattt cggttacttc ttcgggacgc tcgtcaatca agaggacgat
      901 gagttcgact tcgggatagt ttgcggtaac ggcgtgggca atgttttgca gcatcacggt
      961 tttaccgctt ttgggcgggg caaccaagag ggcgcgctga cctttgccga tgggggaaat
     1021 caggtcgata gcacgtccgg tcaggttttc ttcggacttt aagtcgcgtt ccagcttcaa
     1081 ctgttcggtc ggaaacagcg gggtcaggtt ttcaaacagg attttatggc ggcatacttc
     1141 cgggtggtcg ccgttgatgg tatcaagcct gaccagggca aaatagcgtt cgttgtcttt
     1201 tgggacgcgc acgcttcctt cgatggtgtc gcccgtatgc aggttgaagc ggcggatttg
     1261 ggtgggtgag acatagatgt cgtcgggacc ggcaagatag gacgtgtccg cgctgcggag
     1321 gaagccgaag ccgtcgggca ggatttcaag cgtgccggag caggtgaaac cctcgccttt
     1381 tttcatcatc tggcggacga tggcaaatac gaggtcttgt ttgcggaatc ggttggcgtt
     1441 ttcgatgccg tgttcttccg ccaattctaa gagtttggaa atgtgcaggg tttgtaattc
     1501 ggagacgtgc ataataatga tgtattttga agaggaaaaa gacaggcaga tgccgtctga
     1561 aagaagaagc tgaccgttgc cggttgctcg gggaaggggg aattgtaggc agtcggcgcg
     1621 tgggtgtcaa atattatcgc ggacggggca tcggcagaaa atgccgtctg agcggagctg
     1681 cttggaaaaa aatacccccg cgcttttcag gctcgggggt atgggcattg attatttgtt
     1741 caattcattc gccaaataca gccaagtttc gatgacggta tccgggttca gggaaacgct
     1801 ttcgatacct tcctcaacca gccatttggc gaagtccgga tggtcggacg ggccttgacc
     1861 gcagatgccg acatatttgt tctgcttgcg gcaggcggag atggcaaggt gcagcatcac
     1921 tttgacggca gggttgcgtt cgtcaaacga ttcggatacc aagccgctgt cgcggtcgag
     1981 accgagggtc agttgggtca tgtcgttcga gccgatggag aagccgtcga agtattgcag
     2041 gaattgttcc gccaataccg cgttgctcgg cagctcgcac atcataatca ggcgcaggcc
     2101 gtttttgccg cgttccaagc cgttttcttt cagggctttg acaacggctt cggcttcgcc
     2161 caaagtgcgg acgaacggaa tcatgatttc aacgttggtc agacccattt catcgcggac
     2221 gcgtttcaag gctttgcatt ccaaggcgaa acagtctttg aagttgtcgg cgacataacg
     2281 cgccgcgccc cggaagccca acatcgggtt ttcttcatgc ggttcgtata cgttgccgcc
     2341 gaccaggttg gcgtattcgt tggatttgaa gtcggacata cggacgatgg ttttacgcgg
     2401 ataaaccgat gcggccaatg tcgccacgcc ttcggcgatt ttatcgacgt agaagtcgac
     2461 aggggacgcg taaccggcga tacggcgggt aatttccgct tttaattcgt cgtcttgttt
     2521 gtcaaattcc aacaaggctt tcgggtggat accgatttgg cggttgatga taaattccat
     2581 acgcgccaag ccgatgcctt cgctgggcag gttggcgaag ctgaatgcga gttcggggtt
     2641 gccgacgttc atcatgactt tgacgggtgc tttgggcata ttgtccaagg cgacatcggt
     2701 gatctgtacg tccaacaggc cggcatagat gaagccggta tcgccttcgg cgcaggatac
     2761 ggtcacttcc tgaccgtttt ccaagagttc ggtcgcattg ccgcagccga cgacggcagg
     2821 aatacccagt tcgcgcgcga tgatggcggc gtggcaggta cggccgccgc ggttggtcac
     2881 gatggcggaa gcacgtttca tcaccggttc ccaatccggg tcggtcatat cggtaacgag
     2941 tacgtcgccg gcttcgacgg aatccatctc ggaagcatct ttaatcaggc gtaccttgcc
     3001 ctgaccgact ttctgaccga tggcacggcc ttcgcataat acggttttat cgccgttgat
     3061 ggcgaagcgg cgcaggttgc ggctgccttc ttcctgggat ttgacggttt cggggcgggc
     3121 ttgcaggatg tagagtttgc cgtccaagcc gtcgcgtccc cactcgatgt ccatcgggcg
     3181 gccgtagtgt ttttcgatgg tcagtgcgta atgcgccaac tcggtgattt cttcgtcggt
     3241 aatggagaag cggttgcggt cttcttcggg gacatcgacg ttggttaccg atttgccggc
     3301 ttcggctttg tcggtaaaaa tcattttgat gtgtttcgaa cccatggttt tacgcaggat
     3361 ggcgggcttg cccgctttga gcgtgggttt gaacacataa aattcgtccg ggttgaccgc
     3421 accttgtacg acgttttcgc ccagaccgta agaggaggta acaaagacga cttgatcgta
     3481 gccggattcg gtgtcgaggg tgaacatcac acctgatgcg ccgctgtcgg aacgcaccat
     3541 gcgttgaacg ccggcggaaa gggcgacgat atcgtgttcg aagcctttgt ggacacggta
     3601 ggaaatggcg cggtcgttgt acagggaggc gaatacatgg tgcatcgctt ctttaacgtt
     3661 ttccaatccg ttgatgttca agaaggtttc ctgttggccg gcaaatgatg cgtcgggcag
     3721 gtcttcggcg gttgcggaag agcgtacggc aacggaaatg tccgcgccgc cggcatcggc
     3781 aaccattttg ttccatgccg cttcgatttc ggcatcgagc tgttcgggga agggtgtgtc
     3841 caaaatccat tggcggattt ctttgccgac gcgtgccagt tcggcaacgt cttcgacatc
     3901 caattttgcc agtgcggcgg aaatgcgttc gctcagaccg ttgtgtgcga ggaatgcgcg
     3961 gtaggcttcg gctgtggtgg caaagccgcc ggggacgcga acgccttttt cggtcagctg
     4021 actgatcatt tcgcccagcg aggcgttttt accgcccacg cgttcaacat ctgtcatacg
     4081 caggttttca aaccaaatta cgtagttgtc ggccatttgt gtgtccaatc caaaatatgt
     4141 taaaaaagaa acaaatccgc gtgcttattt taagcgattc gttcctctgc tgtcatgtgt
     4201 tttatccgtt ttaaaatcat gatgccgtct gaaaaattgc ggtttcggcg tgtgtagcgg
     4261 tttgaaactt gcagccggta tacttctttt tttgggtatt ttctttgtaa aacaggtggt
     4321 ttgaataggt taatgttttt tctgtttgat ttttttgttt attttttaaa attttctgcc
     4381 aaaaaatact ttatataaat aattttattt caaaattata ttgtgtctgt ttgggtgtaa
     4441 tccgaggtag gcgtgctgcg ggtgctttcc ttgtgtctgc cgctgctgtt atgatgatat
     4501 ttttattctg tatttaagga tggaagatga gcagtccgcg ccatgtgttt tacatttccg
     4561 accgtaccgg tctgactgcc gagaatatcg gcgaggcttt gctgaaccag tttggcaatc
     4621 tgtcgttcaa acgccatacg catccgtttg tcgatacgcc ggaaaaggcg cgcgcggtgg
     4681 tggagaaggt caatcggagc cggcaggaaa acggtcagcg tccgattgcg tttgtcagtg
     4741 tggttgatga cgaaatccgt cggattatca aaggggcgga tgcttttcag attaatttct
     4801 ttgagacttt tttgggactg ttggagaaag aactcaatac cgaagccacg gcatccgagc
     4861 aggggcatca cagtatcggt aatacgaagc gttacgatgc gcgtatggag gcggtaaatt
     4921 tctcattgaa ccacgatgac ggggtcagcg ataagaacct tcaggaagcg gatgtaatct
     4981 tgatgggtgt atcgcgttcg ggcaaaacgc cgacctgcct ttacctcgcc ctgcaatacg
     5041 gcatccgtgc ggcaaactat ccgctgattc ctgacgattt ggaatcggcc gatctgccgc
     5101 gtatggtcaa gccttatagg gataagctgt tcgggttgac catccagccg gaacgtttgc
     5161 aggccatccg ccaagagcgc cgcccgaatt caacttatgc caaaatcgat acatgccgca
     5221 gcgaggtggc agacgcgcag agtatgttca gacggcatgg gattccgttt gcgaatacga
     5281 cggataaatc agtggaagag cttgccgtac acatccttca ggcgtgcaag ctcaaacgca
     5341 ggttttgacg ggttttgatt cggtttgaag gtggaaatgc cgtctgaaat caggtttcag
     5401 acggcagttt tatagtggat taacaaaaat catgacaagg cgacgaagcc gcagacagta
     5461 caaatagtac gga
//
LOCUS       DAWHSK010000123         5461 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.123, whole genome shotgun sequence.
ACCESSION   DAWHSK010000123 DAWHSK010000000
VERSION     DAWHSK010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5461
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.123"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..1164
                     /locus_tag="WIC03_001366"
     CDS             <1..1164
                     /locus_tag="WIC03_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEZ3883855.1"
                     /translation="GFDAPEAIRRHLLKLEGQTVHRLLKLRPPKMQAAFNPVYPLPFD
                     VLVIDEASMLDTALMLQLLKAVKTGARVILLGDENQLPSVGIGAVLSVLSQKTVLDGE
                     THQRLAGFLPEHGFSVSANPPVLAQNTAHLSFSHRFGDNSGIGCLARAAVSGDEGAWA
                     LFDRFPDELEHSECSPNARVERLYRAHKAYWQAVKDGNIEAAYAGISDIVVLAAWRQD
                     AEDFNEAYCRHVRRKMNIPEHQAYFAGEPIMIRQNDYALELFNGDIGLIMEDVGRQGG
                     LAAYFADADGFKKVAVSCLPDFEPAFAMTVHKSQGSEYREVWLLPPSAAPSDEGDDAL
                     SGLSRGLLYTAITRAREKFVFFGGEEAFRQAAVTVKTRQTALGSMLERVFSQE"
     gene            complement(1192..2295)
                     /locus_tag="WIC03_001367"
     CDS             complement(1192..2295)
                     /locus_tag="WIC03_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ3883856.1"
                     /translation="MSSFIFPDNGFEWRNESLQKPPKGWHYVRESGANGILKAAYQNI
                     ATVWQGDFHNAKQVLSAMKKRVRKPAAVRSDADIAALFHAHRMKQAQQSRILNMLAVE
                     IRPGFVLDNKRAPDIRAALLDVYGEADGKPFFLPLNLLLGFMGAHEWHKKGVAVPQLG
                     GSIHVPFGVFSPLRGEYLDLLAHAPSTGFQTALDIGTGSGVLAAILAKQGIPSVIGTD
                     TNPKAVACARANIARLGFEKQVEIRETDLFPEGFVDLIVCNPPWLPAKPTSAVESALY
                     DPESAMLAAFLRDAPKHLNPDGEIRLIISDLAEHLGLRPADFLEKAFIRAGLRVADMI
                     KTKPKHKKAANPSDPLAFARTRETTFLYRLKKA"
     gene            complement(2270..2461)
                     /locus_tag="WIC03_001368"
     CDS             complement(2270..2461)
                     /locus_tag="WIC03_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide synthase"
                     /protein_id="HEZ3883857.1"
                     /translation="MEFDAMPSETFRLQTAWAWKDKAGTKNCSVLPLLAVPSVWFTVL
                     PPYFQNMTQVSHVFFYFSR"
     gene            2546..5008
                     /gene="lon"
                     /locus_tag="WIC03_001369"
     CDS             2546..5008
                     /gene="lon"
                     /locus_tag="WIC03_001369"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEZ3883858.1"
                     /translation="MTQKEKHFEEYAALATLPLRDVVVYPHMVLPLFVGRPKSIAALE
                     NAITREEPVFLLAQTDAAVEEPIAADLYQTGTVAQVLQVLKLPDGTVKVLVEGLYRGR
                     VLTIEDTGGLFVSHIEAVVEEDTGGNTDLEAVRRTLLAQFEQYAKLNKKIPAEIIGSI
                     NGIAENSRLTDTVAAHLQLKLTQRQQILEISEIGKRMEFLLAQLESELDIMQAEKRIR
                     GRVKRQMEKSQREYYLNEQIKAIHKELGEEDENGELDALEAGIKKAGMSKEAEEKCLS
                     ELKKLKMMPPMSAESTVVRNYIDTLLELPWKKKSRVSKDIAKAGLVLDADHYGLEKVK
                     ERILEYLAVQKRMDKLKGPILCLVGPPGVGKTSLGESIAKATGRKYVRMALGGVRDES
                     EIRGHRRTYIGSMPGKILQNMAKAGVKNPLFLLDEIDKLGSDFRGDPASALLEVLDPE
                     QNNKFADHYAEVDYDLSDVMFIATSNSLNIPTPLLDRMEIIRLSGYTEDEKINIAMQY
                     LVPKQMKRNGVKEGELAIEESAVRDIIRYYTREAGVRSLDREIAKICRKVVMQITLDE
                     DKKRLSETKKTSKAKPKAVKVNEKNLHDYLGVRRFDYGVAESENRIGQVTGLAWTEVG
                     GELLTVEAAALPGKGVIQCTGQLGDVMKESVSAAWSVVRSRAESVGLAPDFYEKKDIH
                     VHVPEGATPKDGPSAGIAMTLAMVSAFTKIPVRADVAMTGEITLRGEVLPIGGLKEKL
                     LAALRGGIKHVLIPKDNLKDLEEIPENVKTGLTIHPVKWIDEVLALGLESQPEPWAEP
                     SGAEAAAESASKPKPRSRATKH"
     gene            5190..5459
                     /locus_tag="WIC03_001370"
     CDS             5190..5459
                     /locus_tag="WIC03_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEZ3883859.1"
                     /translation="MNKSELIEAIAQEADISKAAAQKALDATTNAVTTALKQGDTVTL
                     VGFGTFYVGERAERQGRNPKTGEPLTIAAAKTPKFRAGKALKDAL"
BASE COUNT         1413 a         1454 c         1501 g         1093 t
ORIGIN      
        1 ggttttgacg cgccggaggc catccgccgc catttgctca aactggaagg gcaaaccgtc
       61 caccgactgc tgaagctgcg cccgcccaaa atgcaggcgg cgttcaaccc tgtttacccg
      121 ctgccgtttg acgtattggt tatcgatgaa gcctctatgc tggatacggc attgatgctg
      181 caacttttaa aagcggtcaa aaccggcgcg cgcgtgattc tgctggggga tgaaaaccag
      241 ctcccgtccg tcggaatagg ggcggtgctg tccgttttgt cacaaaaaac cgttttggac
      301 ggagaaacgc accaaaggct ggccggcttc cttccggaac acggtttcag cgtcagcgca
      361 aatccacccg tgttggcgca aaacaccgcc catctgtcgt tcagccaccg cttcggcgac
      421 aacagcggca tcggctgcct tgcccgtgcc gccgtatcgg gcgatgaagg ggcgtgggca
      481 ttgtttgacc ggtttccgga cgaactggaa cattcggaat gcagtccgaa cgctcgagtc
      541 gaaaggttgt accgggcgca caaagcctat tggcaggcgg taaaagacgg caatatcgaa
      601 gccgcatacg caggcatctc ggatatcgtc gtcctcgcgg cgtggcggca ggatgcggaa
      661 gacttcaacg aagcctattg ccgccatgta cgccgcaaaa tgaacatacc ggaacatcag
      721 gcctatttcg ccggagaacc gattatgatc agacagaacg actacgcgct tgaactgttc
      781 aacggcgaca tcggattgat tatggaagat gtcggacggc agggcggcct tgccgcctat
      841 tttgccgatg cggacggatt taaaaaggta gcggtaagct gcctgcccga tttcgaaccc
      901 gcattcgcca tgaccgtcca caaaagccaa ggttcggaat atcgggaagt ctggctgctg
      961 ccgccttccg ccgcaccttc ggacgaaggg gacgacgcgc tttcgggctt gagcagaggg
     1021 ctgctttaca cagccattac ccgcgcgaga gagaagttcg tattcttcgg cggggaagaa
     1081 gccttccggc aagctgccgt caccgtcaaa acgcgtcaga cggcgttggg cagtatgctc
     1141 gagcgggtat tttcacaaga ataatccgcc cgaatgccgc gccgccgccc cttatgcctt
     1201 tttcaaacgg tataggaaag tggtttcccg ggttcgcgca aaagcaagcg gatcgctcgg
     1261 attcgcggct tttttgtgct tcggcttggt ttttatcata tcggcaacac gcaaacccgc
     1321 ccggataaat gccttttcca aaaaatcggc agggcgcagc cccaaatgct ctgcaaggtc
     1381 ggaaataatc aggcggattt ctccgtcggg attcagatgt ttcggcgcat cccgcaaaaa
     1441 cgcagccagc atcgcagatt cggggtcgta taacgcggat tcgacggcgg aagtcggctt
     1501 ggcgggaagc cagggcggat tgcagacgat cagatcgaca aacccttcgg gaaacagatc
     1561 ggtttcgcgg atttcaacct gtttttcaaa gcccaaacgg gcaatattgg cacgggcgca
     1621 ggcgacggct ttcggattgg tatccgtgcc gatgacggaa ggaatgccct gtttcgccaa
     1681 aatggcggca agcacgcccg agcccgtccc gatatctaat gccgtctgaa aacccgttga
     1741 cggcgcatgg gcgagcaggt cgaggtattc gccgcgcaac ggcgagaata cgccgaaagg
     1801 aacgtgtatg ctgccgccca gctgcggaac ggcaacccct ttcttatgcc actcgtgcgc
     1861 acccataaac cccagcagca gattgagcgg caggaaaaac ggtttgccgt ccgcctctcc
     1921 gtacacgtcg agcagggcgg cgcgtatatc gggcgcgcgt ttgttgtcca acacaaaacc
     1981 ggggcggatt tcaacggcaa gcatattcag aatacggctc tgctgcgcct gcttcatacg
     2041 gtgggcatgg aaaagggcgg cgatatccgc atcggaacgg acggcggcag gtttgcgaac
     2101 cctcttcttc attgcggaaa gcacctgttt ggcattgtgg aaatcgccct gccagacagt
     2161 tgcaatattt tgataggcag ccttcaaaat gccgtttgca ccgctttcgc ggacataatg
     2221 ccaacctttg ggcggctttt gcagactttc gttgcgccat tcgaacccgt tatcgggaaa
     2281 aataaaagaa gacatgggat acctgcgtca tgttttgaaa atagggcggc agaaccgtaa
     2341 accatacgga tggtacagca aggagcggca acacagaaca gttttttgtt cctgccttgt
     2401 ctttccaagc ccatgccgtc tgaagccgga atgtttcaga cggcatcgca tcaaactcca
     2461 taaataaacc acatatgctt gaaataatac cttcaacccc aatgtacgcg aaaatcggca
     2521 atctgtcaga cacaagagag tacctatgac acaaaaagaa aagcattttg aggaatatgc
     2581 cgccttggca acccttcctt tgcgggatgt cgtcgtttac ccgcatatgg ttctgccgct
     2641 gtttgtcggc agaccgaaat ccatcgccgc actggaaaac gccattaccc gcgaggagcc
     2701 ggttttcctg ttggcgcaaa ccgatgcggc ggtagaagaa ccgattgccg ccgacctgta
     2761 tcagaccggt acggtcgcac aagtcctgca agtgttgaaa ctacccgacg gcacggtaaa
     2821 agtattggtc gaagggctgt atcgcggacg tgttctgact attgaagaca cgggcgggct
     2881 gttcgtttcc catatagagg cggtcgtgga agaagacacg ggcggcaata ccgacctcga
     2941 agccgtgcgc cgcaccctgt tggcgcagtt tgaacaatac gccaaactca ataaaaaaat
     3001 ccccgccgaa attatcggca gcattaacgg cattgccgaa aacagccggc taaccgatac
     3061 agtcgcagcg catttgcagt tgaaactgac gcaacgccaa cagattttgg agatttccga
     3121 aatcggcaaa cggatggaat tcctgctggc acagctggaa tccgaactcg acattatgca
     3181 ggccgaaaaa cgcatacgcg gacgcgtcaa acgccaaatg gaaaaatccc agcgcgaata
     3241 ttatctgaac gaacagatta aagcgataca caaagaattg ggcgaagaag acgaaaacgg
     3301 cgaactggat gccttggaag caggcatcaa aaaggcgggt atgagtaaag aagcggaaga
     3361 aaaatgcctg tccgaactga aaaaactcaa aatgatgccg ccgatgtctg ccgaatccac
     3421 cgtcgtacgc aactacatcg acaccctgct cgaactgccg tggaagaaaa aatcccgcgt
     3481 cagcaaagac atcgccaaag ccggactggt gctggatgcc gaccactacg gcctggaaaa
     3541 agtcaaagaa cggattttgg aatacctcgc cgtccaaaaa cgtatggaca aactcaaagg
     3601 cccgattctg tgcctggtcg gccctccagg cgtgggcaaa acctctttgg gcgaatccat
     3661 cgccaaagca acggggcgga aatatgtccg catggctttg ggcggcgtgc gcgacgaaag
     3721 cgaaatccgg ggacaccgcc gcacctatat cggctccatg cctggtaaga ttctgcagaa
     3781 tatggcaaaa gccggcgtaa aaaacccatt gttcctgctc gacgaaatcg acaaattggg
     3841 tagcgacttc cggggcgatc ccgccagcgc gttgctcgaa gtgcttgatc ccgagcaaaa
     3901 caacaagttc gccgatcatt atgccgaagt ggattacgat ttgagcgatg tgatgtttat
     3961 tgcgacttca aacagcctga atatcccgac cccgctgctc gaccgtatgg aaatcatccg
     4021 tctgtccggc tataccgaag acgaaaaaat caatatcgcg atgcagtacc tcgtgccgaa
     4081 gcaaatgaag cgcaacggcg tgaaagaagg ggaattggca atcgaagaaa gcgcggtgcg
     4141 cgatattatc cgttactaca cccgcgaggc gggcgtgcgt tcgctcgacc gcgagattgc
     4201 caaaatctgc cgcaaggtgg tgatgcagat taccttggac gaagataaga agaggttgtc
     4261 tgaaaccaag aaaaccagca aagccaaacc taaagcggtt aaagtaaatg agaaaaatct
     4321 gcacgactat ttgggtgtgc gccgcttcga ttacggcgtt gccgaaagcg aaaaccgtat
     4381 cggacaagta accggtctgg cgtggacaga agtcggcggc gaactgctga ccgtcgaagc
     4441 cgcagcattg ccgggtaagg gcgtgattca gtgcaccggc cagttgggcg atgtgatgaa
     4501 ggaatccgtg tctgcagcgt ggtcggttgt ccgctcccgc gcggaatcag tgggcttggc
     4561 tcctgatttt tacgagaaaa aagacatcca tgtccatgtt cccgaaggtg cgacgccgaa
     4621 agacggtccg agtgcgggca ttgccatgac tttggcgatg gtgtctgcat tcaccaaaat
     4681 cccggtgcgc gccgatgttg ccatgacggg cgaaattacc ctgcgcggcg aagtcctgcc
     4741 gattggcggt ctgaaggaaa aactgttggc tgcgcttcgc ggcggcatca aacacgtttt
     4801 aattccaaaa gacaacctca aagacttgga agaaatccct gaaaacgtga aaaccggcct
     4861 gaccatccat ccggtcaaat ggatagacga ggtattggct ctgggtttgg aaagccagcc
     4921 tgagccttgg gcagaacctt ctggtgcgga agcggcggcg gaatccgctt caaaaccaaa
     4981 accccgcagc agggcaacca aacattgaaa cgcaggaaat gtgttgtaaa aatgcggttt
     5041 cgtcctgaaa gcctgtcaaa tagggtgatt ccgtattttt tgcttgacac ggcaatttca
     5101 gaattgctat aaagcgaaag ttgctcaagc agtacaaacc ccgagtattc gggcatttca
     5161 tcatttttta agacaggaag ggactgattg tgaacaagtc tgaattgatc gaagcgattg
     5221 ctcaagaagc cgacatttcc aaagccgccg cacaaaaagc tttggatgcc actaccaatg
     5281 cagtaaccac cgccctgaaa caaggcgaca ccgttacttt ggtcggtttc ggtactttct
     5341 acgtgggcga acgtgcggaa cgccaaggcc gcaaccccaa aaccggcgag cctctgacca
     5401 ttgccgccgc caaaacgcct aaattccgcg ccggcaaagc tttgaaagac gcactgtaag
     5461 c
//
LOCUS       DAWHSK010000124         5449 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.124, whole genome shotgun sequence.
ACCESSION   DAWHSK010000124 DAWHSK010000000
VERSION     DAWHSK010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5449
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.124"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            88..513
                     /locus_tag="WIC03_001371"
                     /pseudo
     CDS             88..513
                     /locus_tag="WIC03_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701621.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NosR"
     gene            519..1748
                     /locus_tag="WIC03_001372"
                     /pseudo
     CDS             519..1748
                     /locus_tag="WIC03_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777561.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
     gene            1806..2426
                     /locus_tag="WIC03_001373"
     CDS             1806..2426
                     /locus_tag="WIC03_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ3883860.1"
                     /translation="MTTHHVELRKVTKRFGAQKAVNQVDLVLKAGESVGLAGHNGAGK
                     STIMKLILGLITPTEGEVMLLGERTGSKAGARLRSQIGYLPETVALHPSLTGIETLDF
                     YAKLKKQPLTQNRGLLERVGISQAAHRRVGTYSKGMRQRLALAQALLGEPKVLLFDEP
                     TTGLDPASRQMFYEVVRELNGRGATVLLSTHALAELDGHADRIIVD"
     gene            2437..2568
                     /locus_tag="WIC03_001374"
                     /pseudo
     CDS             2437..2568
                     /locus_tag="WIC03_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688339.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            2565..3059
                     /locus_tag="WIC03_001375"
     CDS             2565..3059
                     /locus_tag="WIC03_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HEZ3883861.1"
                     /translation="MKKTLLAIVAVSALSACRQAEEGSPPLPRQISDRSVGHYCSMNL
                     TEHNGPKAQIFLNGKPDQPVWFSTIKQMFGYTKLPEEPKGIRVIYVTDMGNVTDWTNP
                     NADTEWMDAKKAFYVIDSGFIGGMGAEDALPFGNKEQAEKFAKDKGGKVVGFDDMPDT
                     YIFK"
     gene            complement(3147..4808)
                     /locus_tag="WIC03_001376"
     CDS             complement(3147..4808)
                     /locus_tag="WIC03_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HEZ3883862.1"
                     /translation="MTESITRDSMQYDVVIVGAGPSGLSAAIKLKQLAEQNGREISVC
                     VVEKGSEAGAHSLAGAVIDPIALNELIPDWKEKGAPLTRTVTQDKVLFLTEKKAFNLP
                     ITPNFDNHGNYIVSLGEVVRWLAEQAENLGVEIYPGFAAAEVLYHEDGSVKGIATGNM
                     GIGKDGEPTDSFQPGMELWAQQTLFAEGCRGSLSKQVIERFQLDQNSQPQTYGLGIKE
                     IWEVPSEKHQPGLVVHSAGWPLDSKTYGGAFVYHFDDNKVAVGFVVGLDYQNPYLSPF
                     EEFQRFKTHPEIRKTFEGGRRIAYGARSLIEGGLQSLPKLSFKGGVLIGDAAGFLNMP
                     RIKGIHTAMKSAMLAAEAVFPLLENLEEVENFDSGKEAADYQQRFEQSWLYQELYAAR
                     NVRPSFKWGVYLGSIYTGIDQMIFRGKAPWTLKHHGKDNEQLKKAAACKPIDYPKPDG
                     VLTFDRLSSVFLANLAHEENQPDHLVLNNPQTMIDVNYKEYASPETRYCPAGVYEIVE
                     ENGNPRLQINAANCVHCKTCDIKDPTQNITWICPEGASGPNYGGM"
     gene            complement(4938..5432)
                     /locus_tag="WIC03_001377"
     CDS             complement(4938..5432)
                     /locus_tag="WIC03_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEZ3883863.1"
                     /translation="MDFSCGAASVATLLNNFYGQKLTEEDVLKKLDKEQMPASFEDMR
                     RIMPDLGFEAKGYALSFEQLAQLQIPVIVYLKYRKDDHFSVLRGIDGNTVLLADPSLG
                     HVSMSRAQFLDAWQTREGNLAGKILAVVPKGRDAGGDKAFFTRSPKRQTEFAVGQVKW
                     WRAY"
BASE COUNT         1302 a         1466 c         1393 g         1288 t
ORIGIN      
        1 ggtttaaatt taatccacta tattatcccg tatgcggatt ggttttaaga tttgtaaatt
       61 taatttgcat caaaaaatcg ccgatagatg attcatataa tatcaatatt aaagagtatc
      121 ggtatatcgg ggatagtcat gtcctgtttt tcaatcaaac gtatgtccgc gtttcgggcg
      181 cggataacgg cgttttttgc cgcctttgtc tttttgacgg cggcactgcc cgcttatgcg
      241 gagcgtctgc ctgattttct ggcgaaaata cagccttcgg aaatttttcc gggtgcggac
      301 cgttacggca agccggaagg taagcctatg gttgcccgcg tttacaaagg cgatgagcag
      361 ttgggcttgg tctatatcac gaccgatgcg gtcaatacgc gcggttattc gagcaaaccg
      421 attgatacgc tgatggcgtt ggctaaagac ggtacgatag ccggagcgaa attggttgat
      481 caccatgagt cgattatgct gatcggtatc ccgcatttgc ccgcgcccgg gcgggcgata
      541 cgctcaaact ggcttccggc gtatatcaaa ccaaacttta tatcgacaaa ccgattacga
      601 ttgaagggcc tgccgaccgt tctgcaacca tcgaagggga caggagcggg cgtaccatag
      661 ccgtacacgc gccggacgta acgctccgca acctgaccgt tacccgttcc ggcatgagcc
      721 tgccagcaat ggatgccggt atttatctcg aagaaaccgc tccgcacgcc ctgattgaac
      781 acaataatat tttggataat tcggtcggcg tctatctgca tggttcttcc gaagcgatgg
      841 tgcgcgagaa caaaatcgtc ggcgacgcga ctttgcgcgt gaacgagcgc ggcaacggcg
      901 ttaccgtttg gaacgcgccc ggtgcgcaag tcgtcggcaa cgacatttcc aaagggcggg
      961 acggcatttt ttccaacacc agcacatata acacctacaa aaacaaccgc ttcagcgatt
     1021 tgcgtttcgc cgtccactat atgtacacca acgacagcga aatcagcggc aatatttccg
     1081 tgggcaacaa tatgggctat gtgctgatgt tttccgagcg gctcaaagta ttcgacaata
     1141 tcgccgtcgg cagccgcgat cagggcatta tgctcaacta tgtcaactat tccgatattc
     1201 acgacaacat tatcaacaaa gcgggcaagt gcgtatttgc ctataatgcc aactacgata
     1261 aacttttcgc caatcatttt gaaaactgcc aaatcggcat acactttacc gccgccatcg
     1321 aaggcacgtc cctgcacgac aattccttca tcaacaacga aagccaggtc aaatacgtca
     1381 gcacgcgctt tctcgactgg agcgagggcg gacacggcaa ctattggagc gacaacagcg
     1441 cgttcgattt gaacggcgac ggcttcggcg acagcgcgta ccggcccaac ggcatcatcg
     1501 accaaatcat ctggcgcgcg cccgtatcgc gtctcttgat gaacagtccc gcaatcagca
     1561 tcgtcaaatg ggcgcaggcg cagtttcccg ccgttctgcc tggcggcgtg gtggacagca
     1621 aaccgctgat gaagccttat gcccccaaaa ttcaaacccg ttatcaggcg atgaaggacg
     1681 agttgctcaa agaagccgaa acgcggcagt cggaatgggg cagggcggaa aacgggtctt
     1741 tgaactagtc tgtttcagac ggcatccgga ttcaaatgcc gtctgaaaac acaaaaggaa
     1801 caaccatgac cacacatcat gtcgaattga ggaaggtaac caaacggttc ggggcgcaaa
     1861 aagccgttaa ccaagtcgat ttggttttga aggcaggaga aagcgtcggg cttgccggac
     1921 acaacggcgc gggcaagtcc accattatga agctgatact cgggctgatt accccgaccg
     1981 aaggcgaagt gatgcttttg ggcgaacgta ccggcagcaa agcgggggcg cggcttcgca
     2041 gccaaatcgg ctacctgccc gaaactgttg cgttgcaccc ttcgctgacc ggcatcgaaa
     2101 cgctggattt ttatgccaaa cttaaaaaac agccgctcac gcagaatcgg gggctgctcg
     2161 agcgcgtcgg catttcacag gcggcacacc gccgcgtcgg cacttattct aaagggatgc
     2221 gccaacgcct tgccttggca caagccctgc tgggcgagcc caaagtcctg ctgtttgacg
     2281 aaccgacaac cggcctcgac cctgcatcaa ggcaaatgtt ttacgaagtc gtgcgcgaac
     2341 tcaacgggcg cggcgcgacc gtattgctca gtacccacgc ccttgccgag ttggacgggc
     2401 acgccgaccg cattatcgtg gattaaattt aatccactat atgcgggtat ggcgggtttg
     2461 agcggacaaa tcagcctgac cgtccccgtt ttgctgaccg ctcaggtttt atgggttatc
     2521 attccgcttg ttttggcagc cggaattttt agaaagcgac aaatatgaaa aaaaccctgt
     2581 tggcaattgt tgccgtttcc gccttaagtg cctgccggca ggcggaagag ggatcgccgc
     2641 ctttaccccg gcagattagc gaccgttcgg tcggacacta ttgcagtatg aacctgaccg
     2701 aacacaacgg ccccaaagcc cagattttct tgaacggcaa acccgatcag cccgtttggt
     2761 tctccaccat caagcagatg ttcggctata ccaagctgcc cgaagagcct aaaggcatcc
     2821 gcgtgattta cgttaccgat atgggcaatg ttaccgattg gacgaatccc aatgccgaca
     2881 cggagtggat ggatgcgaaa aaagcctttt acgtcatcga cagcggcttt atcggcggta
     2941 tgggtgcgga agacgcgctg ccgttcggca acaaagagca ggctgagaaa tttgcaaagg
     3001 ataaaggcgg taaggttgtc ggtttcgacg atatgcctga tacctatatt ttcaaataat
     3061 attgaaaaaa ccggccgaaa ctttaaaaac gagtttcggt cggtttttct tttcttgttc
     3121 ggtatagtgt cggcaggaaa gaaccttcac atcccgccgt aattcggccc gctcgcgcct
     3181 tcggggcaaa tccaagtgat gttttgcgtc gggtctttga tatcgcaggt tttgcagtgc
     3241 acgcagtttg ccgcgttgat ttgcaggcgc ggattgccgt tttcttcaac aatttcgtac
     3301 acgccggccg gacaatagcg cgtttcgggc gaggcgtatt ctttgtagtt cacgtctatc
     3361 atcgtttgcg gattgttcag caccaaatgg tcgggctggt tttcttcgtg cgcgagattg
     3421 gcaaggaaga cgctgctcaa gcggtcgaag gtcaacacgc cgtcgggttt cggataatca
     3481 atcggcttgc acgcggcggc ttttttgagc tgctcgttgt ctttgccgtg atgtttcaag
     3541 gtccacgggg ctttgcctct gaaaatcatc tgatcgatgc cggtgtagat tgagccgagg
     3601 taaacgcccc atttgaatga cggacggaca ttgcgcgcgg cgtaaagctc ttgatacagc
     3661 cagctttgtt caaaacgttg ctgataatcc gccgcctctt tgccgctgtc gaagttttcc
     3721 acttcttcaa ggttttccaa caaggggaac acggcttctg cggcgagcat ggcggatttc
     3781 atcgcggtat gaatgccttt gatgcgcggc atattgagga agcctgccgc gtcgccgatt
     3841 aaaacgccgc ctttgaacga gagcttcggc aaactttgca aaccgccttc aatcagcgaa
     3901 cgcgcgccgt aagcgatgcg gcggccgcct tcaaaggttt tgcggatttc gggatgggtt
     3961 ttgaaacgtt ggaactcttc aaacggcgac agataagggt tttgatagtc caaaccgacc
     4021 acgaaaccga cggcgacttt gttgtcatcg aaatggtaaa caaacgcgcc gccgtaggtt
     4081 ttgctgtcca gcggccagcc cgcgctgtgc accaccaagc cgggctgatg tttttcagac
     4141 ggcacttccc aaatttcttt gatgcccaag ccgtaagttt gcggctggct gttttggtcg
     4201 agttggaaac gttcgatgac ttgtttggaa agcgaaccgc gacaaccttc ggcaaacaga
     4261 gtttgctgcg cccaaagctc catgccgggc tggaaactgt cggtcggctc gccgtctttg
     4321 ccaatgccca tattgccggt tgcaatgcct ttgaccgaac cgtcttcgtg atacagcact
     4381 tcggcggcgg caaagcccgg ataaatttcc acgcccaaat tttccgcctg ctccgccaac
     4441 cagcgcacga cttcgcccaa gctgacgatg tagttgccgt gattgtcgaa atttggggta
     4501 ataggcagat tgaacgcttt tttctcagtc aggaacaaca ctttgtcctg cgttaccgta
     4561 cgcgtcagcg gcgcgccttt ttctttccag tcgggaatca actcattcag cgcaatcgga
     4621 tcgataaccg cgcccgccag cgaatgtgca cccgcctccg aacctttctc caccacgcaa
     4681 acgctgattt cgcgcccatt ttgttcggca agctgcttga gtttgatggc ggcggacaaa
     4741 cccgatgggc ctgcgccgac aatcacgaca tcgtattgca tactgtcgcg ggtgatggat
     4801 tctgtcatgg cggttcctgt gtatttatta ttgaattgca aatccgtaat tatacaacga
     4861 gaacatatag ttaccaaata caacaaaggt cgtctgaaaa ccatattttc ggttttcaga
     4921 cgacctttgt cgaaatttca ataagcacgc caccatttta cctgtccgac cgcaaactcc
     4981 gtctgacgtt tcggactgcg tgtgaaaaac gccttatccc cgccggcatc cctccctttc
     5041 ggcacaaccg ccaaaatctt acctgccaaa tttccctcac gggtttgcca agcatccaaa
     5101 aactgcgccc tgctcattga aacatgaccc agcgacgggt cggcaagcaa aaccgtattg
     5161 ccgtctatac cgcgcaatac cgagaaatga tcatccttgc ggtatttcag atacacgatg
     5221 acggggattt gcaactgtgc aagctgctcg aaagacaggg catagccttt cgcttcaaaa
     5281 cccaaatcag gcataatgcg ccgcatatcc tcaaacgacg cgggcatctg ctccttatcc
     5341 agttttttta acacgtcctc ttccgtcagc ttttgcccgt aaaaattgtt caaaagcgtc
     5401 gccaccgaag ccgccccgca ggaaaaatcc aaatcctgct ttacaatat
//
LOCUS       DAWHSK010000125         5440 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.125, whole genome shotgun sequence.
ACCESSION   DAWHSK010000125 DAWHSK010000000
VERSION     DAWHSK010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5440
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.125"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..875)
                     /gene="mafA"
                     /locus_tag="WIC03_001378"
     CDS             complement(<1..875)
                     /gene="mafA"
                     /locus_tag="WIC03_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HEZ3883864.1"
                     /translation="MQARLLIPILFSVFILSACGTLTGIPSHGGGKRFAVEQELVAAS
                     ARAAVKDMDLQALHGRKVALYIATMGDQGSGSLTGGRYSIDALIRGEYINSPAVRTDY
                     TYPRYETTAETTSGGLTGLTTSLSTLNAPARSRTQSDGSGSRSSLGLNIGGMGDYRNE
                     TLTTNPRDTAFLSHLVQTVFFLRGIDVVSPANADTDVFINIDVFGTIRNRTEMHLYNA
                     ETLKAQTKLEYFAVDRTNKKLLIKPKTNAFEAAYKENYALWMGPYKVSKGIKPTEGLM
                     VDFSDIRPYGNHTGNS"
     gene            complement(1059..1778)
                     /locus_tag="WIC03_001379"
     CDS             complement(1059..1778)
                     /locus_tag="WIC03_001379"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEZ3883865.1"
                     /translation="MTQQIKYKRVLLKLSGESLMGSDPFGINHDTIVQTVGEIAEVVK
                     MGVQVGIVVGGGNIFRGVSAQAGSMDRATADYMGMMATVMNALALKDAFETLGIKARV
                     QSALSMQQIAETYARPKAIQYLEEGKVVIFAAGTGNPFFTTDTAAALRGAEMNCDVML
                     KATNVDGVYTADPKKDSSATRYETITFDEALLKNLKVMDATAFALCRERKLNIVVFGI
                     AKEGSLKRVVTGENEGTLVHC"
     gene            complement(1997..2851)
                     /locus_tag="WIC03_001380"
     CDS             complement(1997..2851)
                     /locus_tag="WIC03_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEZ3883866.1"
                     /translation="MAEITAKMVADLRAATGLGMMECKKALVEAEGNFDKAEEILRIK
                     SGAKAGKLAGRTAAEGVLAYAINGNVGALVEVNCETDFVAKDAGFVEFANFVAKTAAE
                     KKPASVEELSELVEAERKAIIAKLGENMSVRRFQVIDTANQLVAYIHGALATEGVLVE
                     YKGSEDVARKIGMHIVAAKPQCVSEAEVDAETVEKERHIYTEQAIASGKPADIAAKMV
                     EGRIRKFLAEITLNGQAFVMNPDQTVAQFAKENGTEVVSFVRYKVGDGIEKAVVDYAA
                     EVAAAAKV"
     gene            complement(2980..3708)
                     /gene="rpsB"
                     /locus_tag="WIC03_001381"
     CDS             complement(2980..3708)
                     /gene="rpsB"
                     /locus_tag="WIC03_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEZ3883867.1"
                     /translation="MSQITMRQMIEAGVHFGHQTRFWNPKMAQYIFGARNKIHIVNLE
                     KTLPMFQDAQEAIRRLVANKGTVLFVGTKRQARDIIREEATRAGMPFVDYRWLGGMLT
                     NYKTVKQSIKRLEEKTAALENAAESGFSKKEILEMQRDVEKLERSLGGIKNMKGLPDA
                     IFVIDTGYQKGTLVEAEKLGIPVIAVVDTNNSPDGVKYVIPGNDDSAKAIRLYCRGIA
                     DAVLEGKNQALQETVAAAQEAAAE"
     gene            complement(3885..4118)
                     /locus_tag="WIC03_001382"
     CDS             complement(3885..4118)
                     /locus_tag="WIC03_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883868.1"
                     /translation="MWHIVAIGYLFVAVMYSAAQPSIARALIYLVFWAVLPTVFTVFV
                     VTVRRRNRLMKQQEQAEFEQQRAQRQKDSGTKP"
     gene            4196..4777
                     /locus_tag="WIC03_001383"
     CDS             4196..4777
                     /locus_tag="WIC03_001383"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEZ3883869.1"
                     /translation="MRNEEKRALRRELRGRRSQMGRDVRAAATVKINRLLKRYIKHGR
                     KIGVYWPMGKELRLDGFVRAAQKRGAKLYLPYIEPRSRRMWFTPYPESGMERERIRGR
                     ARLNVPQFAGRKIRVHGLSVLLVPLVGIDREGYRLGQAGGYYDATLAAMKYRLQAKTV
                     GVGFACQLVDRLSVEAHDLPLDGFVSEAGILCF"
     gene            4838..5428
                     /locus_tag="WIC03_001384"
     CDS             4838..5428
                     /locus_tag="WIC03_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ3883870.1"
                     /translation="MSEKIILPVLSLGGVRLEPLDVHHETGLREAVCDGEVWKLGVTS
                     APHPDRVADYIGTALATRLAFAVVDEEAGRVVGTTAYYHFEPQIPRLDIGFTWYAASA
                     RRTRINTCCKIMLLDYAFDVLACRCVGWRTDILNLASQRAIERLGAEKDGVLRMHMLR
                     KDGSVRDTVVYSMLREDWCKNREILTGRLAGYGVQV"
BASE COUNT         1213 a         1240 c         1517 g         1470 t
ORIGIN      
        1 gagttacccg tatgattgcc gtatggtcgg atatcggaga aatcgaccat taatccttcc
       61 gtcggtttaa ttcctttgct tactttatac ggccccatcc acaatgcgta attttcttta
      121 taggcagctt caaacgcatt ggttttgggt ttgatgagca attttttatt ggttctgtct
      181 actgcgaaat attccagttt tgtttgggct ttcagtgttt cggcattgta taggtgcatt
      241 tcggttctgt tgcgtatcgt tccgaatacg tcgatgttaa taaacacatc tgtatcggca
      301 ttggcaggag aaacaacgtc tatgccgcgc aggaaaaata cggtctgtac caagtgggaa
      361 agaaaggcag tgtcgcgcgg gttagtcgtc aaggtttcat ttcgataatc ccccatcccg
      421 ccaatattta agcccagact gctcctactt ccgctaccgt ctgattgggt gcgcgagcgt
      481 gcaggggcat taagtgtaga taaagaagtg gttaaacctg tcaaaccgcc tgatgttgtt
      541 tcagcggtgg tttcgtaacg tggataggtg taatcggtac ggacggcagg gctgtttatg
      601 tattcgccac gaatcagtgc atcaatggag tagcgacccc ctgtcaaact gcctgaacct
      661 tggtcgccca tagttgcaat gtacaatgca acttttcgtc cgtgtaatgc ctgtaaatcc
      721 atgtctttaa cggcagctct ggcagaagcg gccacaagtt cttgttcgac cgcaaagcgt
      781 ttacctccgc catgcgatgg aatacctgtc agtgtcccgc aggcggataa aataaaaact
      841 gaaaaaagaa taggtatcag cagccgtgct tgcatagatt ttctcctttg atgaaaaaca
      901 aattgtatca aaattgtaaa tatagtggat taaatttaaa ccagtacggc aaggcgaggc
      961 aacgccgtac tggtttaaat ttaatccact ataatctcca tataataaat ggctgtctga
     1021 agcccatatg cgactatatc tgccgacact atggtcaatc agcagtgaac cagcgtacct
     1081 tcgttttcac cggttactac acgtttgagc gagccttctt tagcaatgcc gaatactaca
     1141 atattgagct tgcgttcgcg gcagagggcg aaagcggtgg catccatcac tttgaggttt
     1201 ttcaacaagg cttcgtcaaa agtaatggtt tcgtagcgcg tggcggatga gtcttttttg
     1261 gggtctgcgg tgtacacgcc gtcaacgttg gtggctttga gcatcacgtc gcagttcatt
     1321 tccgcaccgc gcaatgcggc ggcagtgtcg gtcgtgaaga acgggttacc ggtaccggcg
     1381 gcaaaaatca cgactttgcc ttcttccaaa tattggatgg ctttaggacg ggcgtaagtt
     1441 tcagcgattt gctgcataga cagtgcggat tgtacgcgcg ctttgatgcc taaagtttca
     1501 aatgcgtctt tgagtgccaa cgcgttcatc acggtcgcca tcatgcccat gtagtcggcg
     1561 gtggcgcgat ccatgctgcc tgcttgggcg gatacgcccc ggaaaatatt gccgccgccg
     1621 acaacaatac cgacttgcac gcccatttta acgacttcgg caatttcgcc gacagtttga
     1681 acgatggtat cgtgattgat gccgaacgga tcggaaccca tcagggattc gccggagagt
     1741 ttcagtaata cgcgtttgta tttgatttgc tgtgtcatgg gataccttgc tttcttgaat
     1801 gtcgtcggtc ggagtttggg ccgtctgaat ttcaaacggt cggaatatta atgatagggt
     1861 gtgtcggttt attattgttt tgttttcaga cggcctttat attcggtcgt cttaaaatag
     1921 gtaccgtaaa cggttttctc aaaaaaaagc accctgattc gtttggaatc caggtgcttt
     1981 ctttttcata agtgccttac actttggcgg ctgcggctac ttcggctgcg tagtcgacaa
     2041 cggctttttc gatgccatcg cctactttgt agcgtacgaa gctgaccact tcagtgccgt
     2101 tttctttggc gaattgggca acagtttgat cagggttcat cacgaatgct tggccgttca
     2161 gagtgatttc agccaagaat ttgcggatac ggccttcaac cattttagcg gcgatgtcgg
     2221 caggtttgcc ggaagcgatg gcttgttcgg tgtagatgtg gcgttctttt tcaacggttt
     2281 cagcatctac ttcggcttcg cttacgcatt gtggtttggc ggcaacaatg tgcataccga
     2341 ttttgcgtgc tacgtcttca gagcctttgt actcaaccaa tacgccttcg gtcgccaatg
     2401 cgccgtggat gtaggcaacc agttggttgg cagtgtcgat cacttggaag cgacggacag
     2461 acatgttctc gcccaatttg gcgatgatgg ctttgcgttc tgcttcaacc agttcgctca
     2521 gttcttcaac agaagccggt tttttctcgg cagcagtttt cgcaacgaag ttggcaaatt
     2581 ctacgaagcc cgcgtcttta gcaacgaagt cggtttcgca gtttacttca accaatgcgc
     2641 cgacattgcc gttgattgca taagccaata cgccttcggc agcggtacgg ccggccagtt
     2701 taccggcttt cgcaccggat ttgatacgca ggatttcttc ggctttgtcg aagttgcctt
     2761 cggcttcaac caaggctttt ttgcattcca tcatgcccag gccggtagcg gcgcgcagat
     2821 cggcaaccat ttttgcagta atttctgcca ttttgaatct cctagatttt tgggaaatac
     2881 ggaaaggatt ttccgcattc gggaatcgtg tgccgcgaac ggacgttcag acggcatatt
     2941 tgagaaaagg ggcataacgc ccctcttcgg tttgccagat tactcggcag cggcttcttg
     3001 ggcagcggct acggtttctt gcagcgcttg gtttttgcct tccaaaactg cgtcagcgat
     3061 gccgcggcag tacaggcgga tggctttggc ggagtcgtcg ttgccgggga tgacgtattt
     3121 cacgccgtca gggctgttgt tggtatcgac tacggcgata acggggatgc ccagtttttc
     3181 agcctcgacc agagtacctt tttggtagcc ggtatcgata acgaaaatcg cgtcgggcag
     3241 gcctttcatg tttttgatac cgcccaaaga acgttccagt ttttcaacat cgcgttgcat
     3301 ttccagaatt tcttttttgc tgaaaccgct ttcggcagca ttttccaagg ctgcggtttt
     3361 ttcttccagg cgtttgatgg attgcttaac ggttttgtag ttggtcagca taccgcccaa
     3421 ccagcggtaa tcaacgaaag gcataccggc gcgggtcgct tcttcgcgga tgatgtcgcg
     3481 ggcttggcgt ttggtaccta cgaacaatac tgtacctttg ttggcaacca aacgacgtat
     3541 ggcttcttgc gcgtcttgga acatcggcag ggttttttcc aggttgacga tatggatttt
     3601 gttgcgcgca ccgaaaatgt attgtgccat tttcgggttc cagaaacggg tttggtggcc
     3661 gaagtggaca ccggcttcaa tcatctgacg catagtaatt tgagacattt tgatttcctt
     3721 gaaagggtta agggcacaca ttcaatcgca tagaacttga taggcgacgc ctattgtcaa
     3781 gcacccttcg gcgaaagtgg gcataagttt taaaaaataa aagagtgttt ccgaaatgga
     3841 aaactgacgg attatagcgg ataagaggcc gtctgaaaag ggattcaggg ttttgtgccg
     3901 ctgtcttttt gccgttgtgc acgctgctgt tcgaattctg cctgttcctg ttgcttcatc
     3961 aagcggttgc ggcggcggac ggttacgacg aagaccgtga acacggtggg cagcaccgcc
     4021 caaaaaacca aataaatcaa ggcgcgcgca atactcggct gcgcggcgga atacataacg
     4081 gcaacaaaaa gatagccgat ggcgacgatg tgccacattg tgtgcgtcct gtttgaatgt
     4141 taacaaaatc gttataatcg caacattcta ccgcaatcct gaaaggccgt ctgaaatgag
     4201 gaatgaggaa aaacgcgccc tgcgccgcga attgcgcggg cggcgttcgc aaatggggcg
     4261 ggacgtgcgg gcggcggcaa cggtaaaaat taaccgcctg ctcaaacgtt atatcaagca
     4321 cggtcggaaa atcggcgtgt attggccgat gggcaaggaa ttgcgtttgg acggctttgt
     4381 ccgcgcggcg caaaaacgcg gcgcaaaact ttatctgcct tatatcgaac cgcgttcgcg
     4441 gcggatgtgg tttacgccgt atcctgaaag cggaatggaa cgggagcgca tacggggcag
     4501 ggcgaggttg aacgtgccgc agtttgcagg gcgcaaaatc cgcgtgcacg gtttgtcggt
     4561 attgctcgtt ccgcttgtcg gcatagaccg cgaaggctac cgtttggggc aggcaggcgg
     4621 ctattacgat gcgacgcttg cagcgatgaa ataccgtttg caggcaaaaa ccgtgggcgt
     4681 gggctttgcc tgccagttgg tggacagact gtcggtcgag gcgcacgacc tgccgctgga
     4741 cggttttgtg tcggaggcgg ggatattgtg tttttagccg ttcagacggc attttgaacg
     4801 gaaatgccgt ctgaaggtgg aatattggga gaaaaggatg tcggaaaaga taatactgcc
     4861 ggtcttgtcg ttgggtggcg tacggctcga accgttggat gtgcatcatg aaacgggttt
     4921 gcgcgaggcg gtttgcgatg gggaagtttg gaagctggga gtaacgtccg cgccccatcc
     4981 ggatagggtg gcagactata tcgggacagc attggcaacg cgtttggcgt ttgcggttgt
     5041 cgatgaagag gcgggcaggg tggtcgggac aacggcgtat tatcattttg aaccgcagat
     5101 tccgcgtttg gatattggat tcacatggta tgcggcatcg gcacggcgga cacggataaa
     5161 cacctgttgc aaaatcatgc tgttggatta cgcatttgat gttttggctt gccgttgcgt
     5221 gggatggcgc acggatattc tgaaccttgc ctcgcaacgt gccatcgaac ggctgggtgc
     5281 ggaaaaagac ggcgtgctgc gtatgcatat gctccgtaaa gacggcagtg tgcgcgatac
     5341 ggtcgtgtac agtatgctga gggaagattg gtgcaaaaac agggaaatcc tgaccggcag
     5401 gttggcaggg tatggcgtgc aggtgtgagg cagtattttc
//
LOCUS       DAWHSK010000126         5406 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.126, whole genome shotgun sequence.
ACCESSION   DAWHSK010000126 DAWHSK010000000
VERSION     DAWHSK010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5406
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.126"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            61..444
                     /locus_tag="WIC03_001385"
     CDS             61..444
                     /locus_tag="WIC03_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEZ3883871.1"
                     /translation="MEPSSYAAEKKGKGGIRRVINAFGYSIDGIAAAYRYEAAFRQVL
                     WLNVLLVCAAFFWVSETAVRLPLIIASFVSVIVELFNTAVEAAVDHTSTEKHELAKRA
                     KDAGSAAQLFAMLMLAAVWLSALFG"
     gene            559..1032
                     /locus_tag="WIC03_001386"
     CDS             559..1032
                     /locus_tag="WIC03_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEZ3883872.1"
                     /translation="MKKLLAAVMMAGLAGAVSAAGVHVEDGWARTTVEGMKIGGAFMK
                     IHNDEAKQDFLLGGSSPVADRVEVHTHINDNGVMRMREVKGGVPLEAKSVTELKPGSY
                     HVMFMGLKKQLKEGDKIPVTLKFKNAKAQTVQLEVKIAPMSAMDHGHHHGEAHQH"
     gene            1158..2261
                     /gene="mnmA"
                     /locus_tag="WIC03_001387"
     CDS             1158..2261
                     /gene="mnmA"
                     /locus_tag="WIC03_001387"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEZ3883873.1"
                     /translation="MNTTANPSNIIVGLSGGVDSSVTAALLKQQGYQVRGVFMQNWED
                     DDNDEYCSIKQDSFDAIAVADIIGIDIDIVNFAAQYKDKVFAYFLQEYSAGRTPNPDV
                     LCNAEIKFKCFLDYAVGQGADTIATGHYARKEVRNGVHYLLKGLDRNKDQSYFLYRLK
                     PFQLERAIFPLGGLEKPEVRRLAAEFNLPTAAKKDSTGICFIGERPFREFLQKYLPTD
                     NGKMVTPEGKTVGEHVGLMFYTLGQRKGLGIGGAGEPWFVAAKDLTKNELIVVQGHDH
                     PLLYTRSLVMNDLSFTLPERPKAGRYTCKTRYRMADAPCELRYLDEETAELVFDEPQW
                     AVTPGQSAVLYDGDICLGGGIIQTTDKPVIITR"
     gene            2333..4003
                     /locus_tag="WIC03_001388"
     CDS             2333..4003
                     /locus_tag="WIC03_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEZ3883874.1"
                     /translation="MEKIWLDSYEQGVNAEIDITQYGSVSDVFCQSVKKFAGQPAFQN
                     MGKTLTYAETGKLATDFASYLQNVLKLPRGERVAIMMPNVLQYPIALFGILQAGLVAV
                     NTNPLYTPRELEHQLKDSGAAAIIVLENFANTLELVLPRTQIKHVIVASVGEMFGLLK
                     GSLINFIIRKIKKMVPEYRIRETVSFQTALKEGAKHVFQPVALNREDTALLQYTGGTT
                     GVAKGAVLSHGNICANMLQAKEWIKNQLREGKETVIAALPLYHIFALTVNLMIFANAG
                     SKIVLITNPRDMKGFIGELKKQRVNVFIGVNTLFNAMVNRPDFAEVDFSELRLTLGGG
                     MATQKAVAEKWKKITGTPIVEAYGLTEASPGVCCNPLNIESYSGSIGLPVSSTEVELR
                     DANGKEVPVGQPGELWVKGPQVMQGYWNRPEETAKAIDARGFLETGDIAVMDEKGWLK
                     LVDRKKDLVVVSGFNVYPNEIEEVIAHHGKVMEVACIGVPDEKTGEALKVFVVKKDPS
                     LTKEELIAFCRTELTAYKVPKNIEFRDELPKSNVGKILRRELRQSTGK"
     gene            4115..>5406
                     /locus_tag="WIC03_001389"
     CDS             4115..>5406
                     /locus_tag="WIC03_001389"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEZ3883875.1"
                     /translation="MTKFIFVTGGVVSSLGKGIAAASIAAILESRGLNVTMLKLDPYI
                     NVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFIDSTMTRRNSFSTGQVYENVIAKE
                     RRGDYLGGTVQVIPHITDEIKRRIHEGAAGYDVAIVEIGGTVGDIESLPFLEAIRQMR
                     SQLGRSNTLFAHLSYVPYIAAAGEIKTKPTQHTVKEMLSIGLQPDILICRMDREMPAD
                     ERRKIALFCNVEERAIVGSYDVDSIYECPEMLHNQGIDSIITEQLQLNVQQADLTAWK
                     KIVHAIQNPKHTVKIAMVGKYVDLTESYKSLIEALKHAGIHTETDVQITFVDSESIEK
                     NNGDVSMLKDMDAILVPGGFGSRGVEGKIAAVRYARENNVPYLGICLGMQIALIEYAR
                     DVAGLKGANSTEFDLKCAAPVVALIDEWQTADGSVETR"
BASE COUNT         1402 a         1465 c         1382 g         1157 t
ORIGIN      
        1 ggcaacgccg tacaggtttt tgttaatcca ctatattgag aacattttga aaggataccg
       61 atggaacctt cctcctacgc ggcagaaaaa aaaggcaaag gcggcatcag gcgcgtcatt
      121 aacgcattcg gctattcgat agacggcatc gccgccgcct accgttacga agcggcattc
      181 cgtcaggttt tgtggctgaa cgtactgctg gtgtgcgcgg catttttttg ggtttccgaa
      241 accgccgtcc gcctgccgtt gattatcgcg tcttttgtgt cggtcattgt cgaactgttc
      301 aacaccgccg tcgaagctgc cgtcgatcat acttcgaccg aaaaacacga gctggccaaa
      361 cgcgccaaag acgcaggttc tgccgcacaa ttgttcgcga tgctgatgtt ggcggcggtt
      421 tggctgtctg ccctgttcgg gtaaaacgct tgcagcagga tttgtaatcc tttaggatta
      481 gtatgtggta tttattattc acttaatcca ctataagatt aagcacattc cccgtctgat
      541 attataagga gcagaaagat gaaaaaatta ttggcggccg tgatgatggc aggtttggca
      601 ggcgcggttt ccgccgccgg agtccacgtt gaggacggct gggcgcgcac caccgtcgaa
      661 ggtatgaaaa taggcggcgc gttcatgaaa atccacaacg acgaagccaa acaagacttt
      721 ttgctcggcg gaagcagccc cgttgccgac cgcgtcgaag tgcataccca catcaacgac
      781 aacggcgtga tgcggatgcg cgaagtcaaa ggcggcgtgc ctttggaggc gaaatccgtt
      841 accgaactca agcccggcag ctatcacgtg atgtttatgg gtttgaaaaa acaattaaaa
      901 gagggcgaca agattcccgt taccctgaaa tttaaaaacg ccaaagcgca aaccgtccaa
      961 ctggaagtca aaatcgcgcc gatgtcggca atggaccacg gtcatcacca cggcgaagcg
     1021 catcagcact aatctgctga aaatatttga aatgccgtct gaaaaagccc gggctttcag
     1081 acggcatttt tatgaccgcc tttaaaatgt gttaaaatct gcctttaaaa accgccgttt
     1141 ccaaccatat tctacgcatg aatacgacag caaacccttc caatatcatc gtcgggctct
     1201 ccggcggtgt cgattcttcc gtaaccgccg ccctgctcaa gcagcagggt tatcaagtgc
     1261 gcggtgtgtt catgcagaac tgggaagacg acgacaacga cgaatattgc agcatcaaac
     1321 aggattcgtt cgatgccatc gccgttgccg atattatcgg catcgacatc gacatcgtta
     1381 atttcgccgc gcaatataaa gacaaagtct ttgcttattt tcttcaggaa tacagtgcgg
     1441 ggcgcacgcc gaatcccgat gtgttgtgca acgccgaaat caaattcaaa tgctttttgg
     1501 attacgccgt agggcagggc gcggatacca tcgccaccgg acactatgcg cgcaaagaag
     1561 tccgcaacgg cgtgcattac ctgctcaaag gtttggatcg aaacaaagac caaagctatt
     1621 tcctctaccg cctcaagcct ttccaactcg agcgcgcgat ttttccgttg ggcggtttgg
     1681 aaaaacctga agtccgccgc cttgccgccg aatttaattt gccgaccgcc gccaaaaaag
     1741 acagcacggg catctgcttc atcggcgaac gcccgtttcg cgagtttctg caaaaatacc
     1801 tgccgaccga caacggtaaa atggttacgc ccgaagggaa aaccgtcggc gaacacgtcg
     1861 ggctgatgtt ctacacgctc ggacagcgca aaggtttggg catcggcggc gcgggcgaac
     1921 cgtggtttgt tgcggctaaa gatttgacga aaaacgaact gattgtcgtg caaggacacg
     1981 accatccgct gctctatacc cgcagccttg tgatgaacga tttgagtttc acgcttcccg
     2041 aacgcccgaa ggcaggacgc tatacctgca aaacgcgtta ccgtatggcg gacgcgcctt
     2101 gtgagttgcg ctatttggat gaagaaaccg ccgagctggt gtttgacgaa ccgcaatggg
     2161 cggttacgcc gggccagtct gccgtgctgt acgacggcga catctgtttg ggcggcggca
     2221 tcattcaaac gaccgacaag cctgtcatca tcacacgata aaacttatgc cgtctgaaac
     2281 agttttcaga cggcattgtt ccgctcaatt ccactttaaa agaccgatac ctatggaaaa
     2341 aatctggtta gacagttacg aacagggcgt gaatgccgaa atcgacatca cgcaatacgg
     2401 ttccgtcagc gatgtatttt gccaaagtgt caagaaattt gccggccaac ctgcttttca
     2461 aaacatgggc aaaacgctga cttatgccga aaccggcaaa ctggcgaccg atttcgcctc
     2521 ttatctgcaa aacgtcctca agctgccgcg cggcgaacgt gttgccatta tgatgccgaa
     2581 cgtattgcaa tatccgattg cccttttcgg tattttgcag gcaggtttgg tagcggtgaa
     2641 caccaatccg ctctataccc cgcgcgagct ggagcatcag ttgaaagaca gcggcgcggc
     2701 cgccatcatc gttttggaaa atttcgccaa cacgctggag ctggtgctgc cgcgcacgca
     2761 gatcaaacac gtcatcgtcg cctctgtcgg cgaaatgttc gggctgctta aaggttcgct
     2821 gatcaatttc atcatccgga aaatcaagaa aatggtgccc gaataccgta ttcgggaaac
     2881 cgtttccttt cagacggcat tgaaagaagg ggcgaagcac gttttccaac ctgtcgcatt
     2941 aaaccgcgaa gataccgcgc tgttgcaata cacgggcggc acgacaggtg ttgccaaagg
     3001 ggcggtgctg agtcacggca acatctgcgc caatatgctt caggcaaaag aatggattaa
     3061 aaaccagttg cgcgagggca aagaaaccgt tatcgccgcc ctgccgctgt atcatatttt
     3121 tgccttaacc gtgaatctga tgatttttgc caatgccggc tcgaaaattg tcctgattac
     3181 caacccgcgc gatatgaaag gctttatcgg cgaactgaaa aaacagcggg ttaatgtatt
     3241 tatcggcgta aacacgctgt ttaacgcgat ggttaaccgg cccgattttg ccgaagtcga
     3301 tttttcagaa ttgcggctga ctttgggcgg cggtatggcg acccaaaaag ccgttgccga
     3361 aaaatggaaa aaaatcaccg gcacgcccat cgtcgaagcc tacggtttga ccgaagccag
     3421 ccccggcgtg tgctgcaacc ccttaaacat cgaatcatac agcggcagca tcggtttgcc
     3481 cgtctcgtcc accgaagtcg aactgcgcga cgcaaacggc aaagaagtcc ccgtcgggca
     3541 gccgggcgaa ttgtgggtaa aaggccctca agtgatgcaa ggctactgga accgccccga
     3601 agaaaccgcc aaagccatag acgcgcgcgg cttcttggaa accggcgata ttgccgtcat
     3661 ggacgaaaaa ggctggttga agctggtcga tcgtaagaaa gacctcgtcg ttgtttccgg
     3721 attcaatgtt tatccgaacg aaatcgagga agtcatcgcg catcacggca aagtcatgga
     3781 agtcgcctgt atcggcgttc ccgacgaaaa aaccggcgag gcactcaaag tattcgtcgt
     3841 caaaaaagac ccgtcattga ccaaagaaga acttatcgcc ttttgccgca ccgaattaac
     3901 cgcatataaa gtaccgaaaa atatcgaatt ccgcgacgag ttgcccaagt ccaacgtcgg
     3961 caaaatcctg cgccgcgagt tgcggcaaag taccgggaaa taaagaaaag atgccgtctg
     4021 aaaacagccg tccaccgttc agacggcatc cgtccgtttg ccagaaccgc gcgcttcacg
     4081 ttaaaatcac gcattccaac atgggtattc catcatgacc aaattcattt tcgtaaccgg
     4141 cggcgttgtc tcctcactgg gtaaaggtat cgccgccgct tctattgccg ccatcctcga
     4201 atcgcgcggc ttgaacgtta ccatgctcaa gctcgatcct tatatcaacg tcgatcccgg
     4261 cacgatgagc ccgttccaac acggcgaagt gttcgtaacc gacgacggcg cggaaaccga
     4321 cctcgacttg ggacactacg aacggtttat cgattccacg atgacccgcc gcaacagctt
     4381 cagcacgggg caggtgtacg aaaacgtcat cgccaaagaa cgacggggcg actacctcgg
     4441 cggcacggtt caagtcattc cgcacattac cgacgaaatc aaacgccgca tccacgaagg
     4501 cgcggcaggt tatgatgtgg cgattgtcga aatcggcggc acggtcggcg acatcgaatc
     4561 gctgccgttt ttggaagcca tccgccaaat gcgaagccag ttgggacgca gcaacaccct
     4621 gttcgcccac ttgagctacg tcccctacat cgccgccgcc ggcgaaatca aaaccaaacc
     4681 gacccagcat actgttaaag aaatgttgag catcggtttg caaccggaca tcctgatctg
     4741 ccgtatggac agagaaatgc ctgcggacga gcgccgtaag attgcattgt tctgtaacgt
     4801 tgaagaacgt gccatcgtgg gcagttacga cgtggacagc atttacgaat gccctgaaat
     4861 gctgcacaac caaggcatcg acagcattat taccgagcag ttgcagctta atgtgcagca
     4921 ggcggatttg accgcgtgga aaaaaatcgt ccacgccatc caaaacccga aacacaccgt
     4981 caaaatcgcc atggtcggca aatacgtcga tttgaccgag tcctacaaat cgctgatcga
     5041 agccttgaaa cacgcaggca ttcacaccga aaccgacgta caaattacct ttgttgacag
     5101 cgaaagcatc gagaaaaaca acggcgacgt ttctatgctt aaagatatgg acgccatcct
     5161 cgttcccggc ggttttggtt cgcgcggcgt ggaaggcaaa atcgccgctg tgcgctatgc
     5221 ccgcgaaaac aacgtgccat acttgggcat ctgcctcggt atgcagattg cgctgatcga
     5281 atacgcccgc gacgtggcag gtttgaaagg tgcgaattcc accgagttcg acctcaaatg
     5341 cgccgccccc gtcgttgccc tgattgacga atggcaaact gccgacggca gcgtcgaaac
     5401 ccgcga
//
LOCUS       DAWHSK010000127         5283 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.127, whole genome shotgun sequence.
ACCESSION   DAWHSK010000127 DAWHSK010000000
VERSION     DAWHSK010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5283
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.127"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            195..2348
                     /locus_tag="WIC03_001390"
     CDS             195..2348
                     /locus_tag="WIC03_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ3883876.1"
                     /translation="MNNPLVNQAAMVLPVFLLSACLGGGGSFDLDSVDTEAPRPAPKY
                     QDVSSEKPQAQKDQGGYGFAMRFKRRNWHPRANPKEDEVKLKNDDWEATGLPTEPKKL
                     PLKQESVISKVETNGDSKIYTSPYLSQDADSSHANGANQPKNEVTDYKNFKYVYSGWF
                     YKHARNEIKAENGLVSAKRGDDGYIFYHGQEPSRQLPASRTVTYKGVWHFVTDTKQGQ
                     KFNDIIQPSKRQGDSYSGFSGDDDEQYSNKNEKTLQGGQEGYGFTSNLEVDFGNKKLK
                     GNLIRNNRVTNATANDKYTTQYYSLEAQVTGNRFNGKAIATDKPQENGTKQHPFVSDS
                     SSLSGGFFGPQGEELGFRFLSDDQKVAVVGSAKTKDKPANGNTAAASGGTDAAASGSA
                     AAMPSENGKLTTVLDAVELTLNDKKIKNLDNFSNAAQLVVDGIMIPLLPEASESGKNQ
                     ANQGTNGGTAFTYKTTYTPESDKKDAQAQTGAGGAQAASGAAGVNGGQAGTKTYEVEV
                     CCSNLNYLKYGMLTRKNSKSAMQAGGNSSQADAKTEQVEQSMFLQGERTDEKEIPNDQ
                     NVVYRGSWYGHIANDTSWSGNASDREGGNRADFTVNFGTKKINGTLTAENRQQATFTI
                     EGDIKDNGFEGTAKTADSGFDLDQSNNTRTPKAYITDAKVQGGFYGPKAEELGGWFAY
                     SSDKQTKNAPDASGNGNSASSATVVFGAKRQQPVQ"
     gene            2434..5181
                     /gene="tbpA"
                     /locus_tag="WIC03_001391"
     CDS             2434..5181
                     /gene="tbpA"
                     /locus_tag="WIC03_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein TbpA"
                     /protein_id="HEZ3883877.1"
                     /translation="MQQQHLFRLNILCLSLMTALPVYAENVQAGQAQEKQLDTIQVKA
                     KKQKTRRDNEVTGLGKLVKTADTLSKEQVLDIRDLTRYDPGIAVVEQGRGASSGYSIR
                     GMDKNRVSLTVDGLAQIQSYTAQAALGGTRTAGSSGAINEIEYENVKAVEISKGSNSV
                     EQGSGALAGSVAFQTKTADDVIGEGRQWGIQSKTAYSGKNRGLTQSIALAGRIGGAEA
                     LLIHTGRRAGEIRAHEDAGRGVQSFNRLVPVEDSSTYAYFVVEEECPNGYAACKDKPK
                     KDVAGKDERQTVSTRDYTGPNRFLADPLSYESRSWLFRPGFRFENKRHYIGGILERTQ
                     QTFDTRDMTVPAFLTKAVFDENRKYGPIRGNGKYAGDHKYGGLFTNGENGALVGAEYG
                     TGVFYDETHTKSRYGLEYVYTNADKDTWADYARLSYDRQGIGLDNHFQQTHCSADGSD
                     KYCRPSADKPFSYYKSDRVIYGESHRLLQAAFKKSFDTAKIRHNLSVNLGFDRFGSNL
                     RHQDYYYQNAKRAYSLKTPPKNGNGGSNPNPNGDKDKPYWVSIGGGNVVTGQICLFGN
                     NTYTDCTPRSINGKSYYAAVRDNVRLGRWADVGAGLRYDYRSTHSDDGSVSTGTHRTL
                     SWNAGIVLKPADWLDLTYRTSTGFRLPSFAEMYGWRAGVQSKAVKIDPEKSFNKEAGI
                     VFKGDFGNLEASWFNNAYRDLIVRGYEAQIQNGKETAKGDPAYLNAQSARITGINVLG
                     KIDWNGVWDKLPEGWYSTFAYNRVHVRDIKKRADRTDIQSHLFDAIQPSRYVVGLGYD
                     QPEGKWGVNGMLTYSKAKEITELLGSRALLNGNSRNTKATARRTRPWYIVDVSGYYTI
                     KKHFTLRAGVYNLLNYRYVTWENVRQTAGGAVNQHKNVGVYNRYAAPGRNYTFSLEMK
                     F"
BASE COUNT         1536 a         1307 c         1347 g         1093 t
ORIGIN      
        1 ctttcgtggg aatgacggga tatacgtggg aatgacggga tataggtttc cgtgcggacg
       61 cgttcggatt cacgactgcg cgggaatgac gggattttgg tgtattccct aaaaaaataa
      121 aaaagtattt gcaaatttgt taaaaataaa taaaataata atccttatca ttctttaatt
      181 gaattggatt tattatgaac aatccattgg tgaatcaggc tgctatggtg ctgcctgtgt
      241 ttttgttgag tgcttgtttg ggcggcggcg gcagtttcga tcttgattct gtcgataccg
      301 aagccccgcg tcccgcgcca aagtatcaag atgtttcttc cgaaaagccg caagcccaaa
      361 aagatcaagg cggatacggt tttgcgatgc gcttcaagcg gcggaattgg catccgaggg
      421 cgaatcctaa agaagatgag gttaaattaa agaatgatga ttgggaggcg acaggattgc
      481 cgacagaacc caagaaactg ccattaaaac aagaatccgt catttcaaaa gtagaaacca
      541 acggtgattc taaaatctac acttcacctt atctcagtca agatgcagat agtagtcatg
      601 caaatggtgc aaaccaacca aaaaacgaag taacagatta caaaaatttc aaatatgttt
      661 attccggctg gttttacaaa cacgcgagaa acgaaatcaa agccgaaaac ggattagtaa
      721 gtgcaaaaag aggcgacgac ggctatatct tttatcacgg tcaagaacct tcccgacaac
      781 ttcccgcttc tagaacagtt acctacaaag gtgtgtggca ttttgtaacc gatacgaaac
      841 agggacaaaa atttaacgat attatccagc cttcaaaaag gcaaggcgac agctacagcg
      901 gattttcggg cgatgatgat gaacaatatt ctaataaaaa tgaaaagact ttacaaggcg
      961 gtcaagaggg ttatggtttt acctcaaatt tagaagtgga tttcggcaat aaaaaattga
     1021 agggtaattt aatacgcaat aatagagtta caaacgctac tgctaacgat aaatacacca
     1081 cccaatacta cagccttgag gctcaagtaa caggcaaccg tttcaacggc aaggcaatag
     1141 cgaccgacaa accacaagag aatggaacca aacaacatcc ctttgtttcc gactcgtctt
     1201 ctttgagcgg cggctttttc ggcccgcagg gtgaggaatt gggtttccgc tttttgagcg
     1261 acgatcaaaa agttgccgtt gtcggcagcg cgaaaaccaa agacaaaccc gcaaatggca
     1321 atactgcggc ggcttcaggc ggcacagatg cggcagcatc gggcagtgcg gcagctatgc
     1381 cgtctgaaaa cggtaagctg accacggttt tggatgcggt tgaattgaca ctaaacgaca
     1441 agaaaatcaa aaatctcgac aacttcagca acgccgccca actggttgtc gacggcatta
     1501 tgattccgct cctgcccgag gcttccgaaa gtgggaaaaa tcaagccaat caaggtacaa
     1561 acggcggaac agcctttacc tacaaaacaa cctacacgcc ggaaagtgat aaaaaagacg
     1621 cccaagccca aacaggcgcg ggcggcgcgc aagctgcttc gggtgcggcg ggcgttaacg
     1681 gcgggcaggc aggaacaaaa acctatgaag ttgaagtctg ctgttccaac ctcaattatc
     1741 tgaaatacgg aatgttgacg cgtaaaaaca gcaagtccgc gatgcaggca ggaggaaaca
     1801 gtagtcaagc tgatgctaaa acggaacaag ttgaacaaag tatgttcctc caaggcgagc
     1861 gtaccgatga aaaagagatt ccaaacgacc aaaacgtcgt ttatcggggg tcttggtacg
     1921 ggcatattgc caacgacaca agctggagcg gcaatgcttc agatagagag ggaggcaaca
     1981 gggcggactt taccgtgaat ttcggtacga aaaaaattaa cggcacgtta accgctgaaa
     2041 acaggcagca ggcaaccttt actattgagg gcgatattaa ggacaacggc tttgaaggta
     2101 cggcgaaaac tgctgactca ggttttgatc tcgatcaaag caataacacc cgcacgccta
     2161 aggcatatat cacagatgcc aaggtgcagg gcggttttta cgggcctaaa gccgaagagt
     2221 tgggcggatg gtttgcctat tcgagcgata aacaaacgaa aaatgcacca gatgcatccg
     2281 gcaatggaaa ttcagcaagc agtgcaactg tcgtattcgg tgcgaaacgc caacagcctg
     2341 tgcaataagc acggttgccg aacaatcaag aataaggctt cgacggcatc gctccttccg
     2401 atgccgtctg aaagcgaaga ttagggaaac attatgcaac agcaacattt gttccgatta
     2461 aatattttat gcctgtcttt aatgactgcg ctgcccgttt atgcagaaaa tgtgcaagcc
     2521 ggacaagcac aggaaaaaca gttggatacc atacaggtaa aagccaaaaa acagaaaacc
     2581 cgccgtgata acgaagtaac cggtttgggc aaattggtca aaaccgccga caccctcagc
     2641 aaggaacagg tactcgatat ccgcgacctg acgcgttacg accccggcat cgccgtggtc
     2701 gaacaggggc gcggcgcaag ctctggctac tcgatacgcg gtatggacaa aaaccgcgtt
     2761 tccttgacgg tggacggctt ggcgcaaata cagtcctaca ccgcgcaggc ggcattgggc
     2821 gggacgagga cggcgggcag cagcggcgca atcaatgaaa tcgagtatga aaacgtcaag
     2881 gctgtcgaaa tcagcaaagg ctcaaactcg gtcgaacaag gcagcggcgc attggcgggt
     2941 tcggtcgcat ttcaaaccaa aaccgccgac gatgttatcg gggaaggcag gcagtggggc
     3001 attcagagta aaaccgccta ttccggcaaa aaccgggggc ttacccaatc catcgcgctg
     3061 gcggggcgca tcggcggtgc ggaggcttta ctgatccaca ccgggcggcg cgcgggggaa
     3121 atccgcgcac acgaagatgc cggacgcggc gttcagagct ttaacaggct ggtgccggtt
     3181 gaagacagca gtacctatgc ctattttgtg gtcgaagaag aatgccctaa tggatatgcg
     3241 gcttgtaaag acaaaccgaa aaaagatgtt gccggcaaag acgaacgtca aacggtttct
     3301 acccgagact acacgggtcc caaccgcttc ctcgccgatc cgctttcata cgaaagccga
     3361 tcgtggctgt tccgcccggg ttttcgtttt gaaaacaaac ggcactacat cggcggcata
     3421 ctcgaacgca cgcaacaaac tttcgacacg cgcgatatga cggttccggc attcctgacc
     3481 aaggcggttt ttgatgaaaa tagaaaatac ggaccgataa gaggcaacgg caaatatgcg
     3541 ggcgaccaca aatacggcgg actgtttacc aacggcgaaa acggtgcgct ggtgggcgcg
     3601 gaatacggta cgggcgtgtt ttacgacgag acgcacacca aaagccgcta cggtttggaa
     3661 tatgtctata ccaatgccga taaagacact tgggcggatt atgcccgcct ctcttacgac
     3721 cggcagggca tcggtttgga caaccatttt cagcagacgc actgttctgc cgacggttcg
     3781 gacaaatatt gccgtccgag tgccgacaag ccgttttcct actacaaatc cgatcgcgtg
     3841 atttacgggg aaagccacag gctcttgcag gcggcattca aaaaatcctt cgataccgcc
     3901 aaaatccgcc acaacctgag cgtgaatctc gggtttgacc gctttggctc taatctccgc
     3961 catcaggatt attattatca aaatgccaaa cgcgcctatt cgttgaaaac gccccctaaa
     4021 aacggcaacg gcggctcaaa ccccaacccc aacggcgaca aggacaaacc ctattgggtc
     4081 agcataggcg ggggaaatgt ggttacgggg caaatctgcc tctttggcaa caatacttat
     4141 acggactgca cgccgcgcag catcaacggc aaaagctatt acgcggcagt tcgggacaat
     4201 gtccgtttgg gcaggtgggc ggatgtcggc gcgggcttgc gctacgacta ccgcagcacg
     4261 cattcggacg acggcagcgt ttccaccggc acgcaccgca ccctgtcctg gaacgcaggc
     4321 atcgtcctca aacctgccga ctggctggat ttgacttacc gcacttcaac cggcttccgc
     4381 ctgccctcgt ttgcggaaat gtacggctgg cgggcgggtg ttcaaagcaa ggcggtcaaa
     4441 atcgatccgg aaaaatcgtt caacaaagaa gccggcatcg tgtttaaagg cgatttcggc
     4501 aacttggagg caagttggtt caacaatgcc taccgcgatt tgattgtccg gggttatgaa
     4561 gcgcaaattc aaaacggcaa agaaacagcc aaaggcgacc cggcttacct caatgcccaa
     4621 agcgcgcgga ttaccggcat caatgttttg ggcaaaatcg attggaacgg cgtatgggat
     4681 aaattgcccg aaggttggta ttctacattt gcctataatc gtgtccatgt ccgcgacatc
     4741 aaaaaacgcg cagaccgcac cgatattcaa tcacacctgt ttgatgccat ccaaccctcg
     4801 cgctatgtcg tcggcttggg ctatgaccaa ccggaaggca aatggggtgt gaacggtatg
     4861 ctgacttatt ccaaagccaa ggaaatcaca gagttgttgg gcagccgtgc tttgctcaac
     4921 ggcaacagcc gcaatacaaa agccaccgcg cgccgtaccc gcccttggta tattgtggat
     4981 gtgtccggtt attacacgat taaaaaacac ttcaccctcc gtgcgggcgt gtacaacctc
     5041 ctcaactacc gctatgttac ttgggaaaat gtgcggcaaa ctgccggcgg cgcagtcaac
     5101 caacacaaaa atgtcggcgt ttacaaccga tatgccgccc ccggtcgcaa ctacacattt
     5161 agcttggaaa tgaagtttta aacgtccaaa cgccgcaaat gccgtctgaa aggcttcaga
     5221 cggcattttt tacacaattc ccgccatttt ccatcattcc caacaacacc gtaatcctga
     5281 aat
//
LOCUS       DAWHSK010000128         5264 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.128, whole genome shotgun sequence.
ACCESSION   DAWHSK010000128 DAWHSK010000000
VERSION     DAWHSK010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5264
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.128"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..342
                     /locus_tag="WIC03_001392"
     CDS             <1..342
                     /locus_tag="WIC03_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pretoxin"
                     /protein_id="HEZ3883878.1"
                     /translation="LTVADWHTYFVKGNQAETEGVWVHNDCPTKPKPTNHAQQRKEEA
                     KNDSHRSVGDSNRVVREGKQYLDADTGNHVSVKGEKVVILTPDGRQVTQFKNSKANTS
                     KRVKNGKWTPK"
     gene            320..601
                     /locus_tag="WIC03_001393"
     CDS             320..601
                     /locus_tag="WIC03_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883879.1"
                     /translation="MENGHRNNRSPLEKRIFYLEHSGQYLMICALSDYSKNKHTVVMA
                     NFLYPNEKMDWRNLDDLFNELVLEELQSSFMDWYPTIEEAISHHLEDFS"
     gene            657..830
                     /locus_tag="WIC03_001394"
                     /pseudo
     CDS             657..830
                     /locus_tag="WIC03_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503800.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pretoxin"
     gene            811..1266
                     /locus_tag="WIC03_001395"
     CDS             811..1266
                     /locus_tag="WIC03_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883880.1"
                     /translation="MVDAVVKTPEFVPFTSVGIFRFGADITEYKNILETFMYEPPDEF
                     GTEYYESPDSNLLISVEKNKIISIFCYQELYFMGVNIIGLNFEDFKQLFHHPKSYGVD
                     KYYLSNESYPTYVYEFDEIGVQAWEAKGKIVTIIAGGKDNYSTEPYYDE"
     gene            1273..1329
                     /locus_tag="WIC03_001396"
                     /pseudo
     CDS             1273..1329
                     /locus_tag="WIC03_001396"
                     /inference="COORDINATES: protein motif:HMM:NF026864.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1344..1721
                     /locus_tag="WIC03_001397"
     CDS             1344..1721
                     /locus_tag="WIC03_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 41 family protein"
                     /protein_id="HEZ3883881.1"
                     /translation="MCELKNFRRNITCFEGYDENSFIGKWYDDGVWDDEEYWKLENDL
                     IEVRRKYPYPMDIPRDIVIGIGTIIDFLMVPNWELFEIKASPWLPDSVGIHERYERFT
                     TMLRYIFTEKDIVNVRFDYYNKK"
     gene            1851..2621
                     /locus_tag="WIC03_001398"
     CDS             1851..2621
                     /locus_tag="WIC03_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HINT domain-containing protein"
                     /protein_id="HEZ3883882.1"
                     /translation="MVKTADGYKAIAHIQAGDRVFAKDETSGKTGYKPVTAQYGNPYR
                     ETVYIEISDGIGNSQTLISNRIHPFYSQGKWIQAGRLKKGDTLLSESGAKQTVQNITL
                     KQQPLKAYNLTVADWHTYFVKGDRAETEGVWVHNDCPYGSSSAKGKSGSERNTAKRGT
                     FRQETLKNAWDLAADGSQPNSKKCPTCGKDVYGNPHNKEKRNTKDGWDGSHNPSWSNR
                     QHSSDRKEQLNDYNKGVSLECRHCNRSGGNKDERFKKK"
     gene            2618..3163
                     /locus_tag="WIC03_001399"
     CDS             2618..3163
                     /locus_tag="WIC03_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HEZ3883883.1"
                     /translation="MIIVEHLEHYLGEIESGIKCLDRRYHLSVSVFPSQPYTGVTTFS
                     TLGLNRYDLNYKSRFELIFTCSEEWNKENIAAFLSGVAEYLIDNRKPILRGEIIQLPR
                     VIIEGSKMDALYVSAPFYFDDDFQVCYGEHYNTVFPLLVPLYKQEAELVEKKGWNAFE
                     QFLLDNEVDNLSDMNRKPFVW"
     gene            3299..3997
                     /locus_tag="WIC03_001400"
     CDS             3299..3997
                     /locus_tag="WIC03_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HINT domain-containing protein"
                     /protein_id="HEZ3883884.1"
                     /translation="MVKTADGYKAIARIRVGDRVFAKDETSGVTGYKPVTARYGNPYQ
                     ETVYIEISDGIGNNQTLISNKIHPFYSQGKWIQAGRLKKGDTLLSESGAKQTVQNITL
                     KQQPLKAYNLTVADWHTYFVKGNQAETEGVWVHNSCPPYKRPNNATTKAQRESVQGKP
                     CVECGKLAEKMIADHKKPLVVEYYETGTIDKSKMRAIESVQPQCPTCSAKQGGRLSQY
                     SKEQKRKLSNGNKK"
     gene            3981..4334
                     /locus_tag="WIC03_001401"
     CDS             3981..4334
                     /locus_tag="WIC03_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883885.1"
                     /translation="MGIKNEQIIICKKYNTEIYPVSDVSKIGVAENVKQANLYPINGL
                     RHRPKGDTNGWYIWAGENFSHDENFFLPLHTFHLQIWRPEIIPFLTLPPGYRFLIGEN
                     GYEDVWFDELLLNDN"
     gene            4324..4647
                     /locus_tag="WIC03_001402"
     CDS             4324..4647
                     /locus_tag="WIC03_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HEZ3883886.1"
                     /translation="MIIDEKSTSDVLKQYQELGFDLTKPMIIEFFIGGSQKNLQSIES
                     QLISYRQDFQISIEQNEFGEMWTCYCSVNIIPSLENILAIETTLFGIAQKNDCNYEGF
                     GSYGN"
BASE COUNT         1813 a          951 c         1086 g         1414 t
ORIGIN      
        1 ctgaccgttg ccgactggca tacctatttc gtcaagggta atcaggcgga aacggaaggc
       61 gtttgggttc ataatgattg cccgaccaaa ccaaaaccaa ctaatcatgc ccaacaaaga
      121 aaagaagaag ctaaaaacga ttctcatcga agtgtgggag attccaatcg tgtcgttcgc
      181 gaaggaaagc aatatttaga tgccgacaca ggaaaccatg tttctgtaaa aggagaaaaa
      241 gtggttattc taactcctga tggaagacag gtaactcaat ttaagaactc gaaagccaat
      301 acgtcaaaaa gggtaaaaaa tggaaaatgg acaccgaaat aatcgatctc ccttagaaaa
      361 aagaattttt tatctggagc attcgggaca gtatctgatg atttgcgccc tatcagatta
      421 ctccaaaaat aaacatactg tcgttatggc aaattttctc tatccaaatg aaaaaatgga
      481 ttggagaaat ttagatgatt tgttcaatga gttggtttta gaggaattac agtcttcatt
      541 tatggattgg tatccaacta ttgaagaggc aattagccat catttagaag atttttcgta
      601 acaagcataa atcaaaaagt cgtctgaaac tgcttttaat tttcagacga ccttgagatg
      661 gcggtcgaag tcaaatatca gcggaatcaa aagttgctga attagaaaat aaaattattc
      721 ccggaatgcc caaaaatgaa cgcttaaaga ttaagcaaaa aatcagaaat attacaaaaa
      781 atgccaatcg aaaagcaaaa ggagaagagc atggtcgacg cggtcgttaa aactcccgaa
      841 tttgtacctt ttacttctgt aggaatattc cgttttggtg cggatataac agagtacaaa
      901 aacatactgg aaacttttat gtatgaacct cctgatgagt ttggaacaga atattatgaa
      961 tctcccgact caaatttact catttccgtc gaaaaaaaca aaattatttc gatattttgt
     1021 tatcaggagc tatattttat gggcgttaat ataataggtc tgaattttga agattttaaa
     1081 cagcttttcc atcaccctaa atcatacggt gttgataaat attatttatc aaatgaatct
     1141 tatccgacat atgtctatga gtttgatgaa ataggagtac aagcttggga agcaaaaggg
     1201 aaaattgtta ctatcattgc cgggggcaaa gacaattatt caacagaacc ttactacgat
     1261 gaataacatt acagaaaaga aacaagaaac ggtacatttg ataaaaacct aaatcgtatt
     1321 ggagattaat tattatgtgt attatgtgtg agttaaaaaa cttcagaaga aatattactt
     1381 gttttgaggg atatgacgaa aattcattta ttggcaaatg gtatgatgac ggggtgtggg
     1441 atgatgaaga atattggaag ctggagaatg atttaatcga ggttaggaga aaatatcctt
     1501 atccgatgga tataccaagg gatattgtga ttggaatcgg taccattatt gattttttaa
     1561 tggttccaaa ttgggagctt tttgaaatta aagcttcccc ttggttgcct gatagtgtgg
     1621 gaattcatga acgttatgaa agattcacaa cgatgctccg ttatattttt accgagaaag
     1681 acatagtcaa cgtgcgattt gattattaca acaaaaaata gcaaaacccg gcaatcaaaa
     1741 tgccgtctga agcgatattt gcttttcaga cggcattttg atgaatgaaa caggatattg
     1801 agaactaagt tcttcaaaaa tcctacatct gctccttcca cagcagcacc ttggttaaaa
     1861 cggcagacgg ttacaaagcc attgcccata ttcaagccgg cgaccgcgtc ttcgccaaag
     1921 atgaaaccag tggaaaaact ggatacaaac ctgttaccgc ccaatacggc aatccgtatc
     1981 gggaaaccgt ttacattgaa atttcagacg gcatcggtaa cagccaaacc ctgatttcca
     2041 accgcatcca tccgttttac agtcaaggaa aatggataca ggcaggtcgt ctgaaaaaag
     2101 gcgacaccct gctttccgaa agcggtgcaa aacagacagt tcaaaacatt accctcaaac
     2161 agcagccgct caaagcctac aatctgaccg ttgccgattg gcatacttac ttcgtcaagg
     2221 gtgatagggc ggaaacggaa ggggtttggg ttcataatga ttgtccgtat ggtagttcta
     2281 gcgcaaaagg taaaagtgga agtgagagaa atactgctaa acgtggaacc ttcagacaag
     2341 aaacgctaaa aaatgcatgg gatttggcag cagatggtag ccagcctaat tcaaaaaaat
     2401 gtcctacatg tggtaaagat gtatatggta atccccataa caaggagaaa cgtaatacaa
     2461 aagatggttg ggatggaagt cataatccat catggagtaa ccgtcaacat agttctgatc
     2521 gtaaggagca actaaatgat tataataaag gagtaagttt agaatgcaga cattgcaatc
     2581 gatccggtgg caacaaggat gaaaggttta agaaaaaatg attatagttg aacatttaga
     2641 gcattatttg ggtgaaatcg aaagcggtat aaaatgcttg gatcgacgtt atcatctcag
     2701 cgtttccgta ttcccatcac aaccatatac aggggtaact actttttcta ctttaggctt
     2761 gaatcgatat gacttgaact ataagagtag gtttgaatta atttttacct gttctgaaga
     2821 atggaataaa gaaaatattg cagcttttct atcaggagta gccgaatatt tgattgacaa
     2881 tagaaaacct attttaagag gtgaaattat tcaattaccc agggtaatta tcgaaggtag
     2941 caaaatggat gcactgtatg tttccgcccc attttatttc gatgatgatt ttcaggtctg
     3001 ttatggcgaa cactacaata ccgttttccc tttgcttgtc ccgttgtaca aacaggaagc
     3061 ggaattggtg gaaaaaaagg gttggaatgc ttttgagcag tttttgctgg ataatgaagt
     3121 tgacaacctt tcggatatga ataggaaacc gtttgtttgg taaaggattt tcttgaattt
     3181 gattaaaggt cgtctgaaat taagcttgtt tttcagacga cctttccttc atttgaaaca
     3241 gaatattgag aactgagttc ttcaaaaatc ctacacctgc tccttccacg gcagcacctt
     3301 ggtcaaaacg gcagacggct acaaagccat cgcccgtatc cgagtcggcg accgcgtctt
     3361 tgccaaggac gaaacaagcg gggtaacggg atacaaaccc gttaccgccc gatacggcaa
     3421 tccgtatcaa gaaaccgttt acattgaaat ttcagacggc atcggcaaca accaaaccct
     3481 gatttccaat aaaatccacc cgttttacag tcaaggaaaa tggatacagg caggtcgtct
     3541 gaaaaaaggc gacaccctgc tttccgaaag cggtgcaaaa cagacggttc aaaacattac
     3601 cctcaaacag cagccgctca aagcctacaa tctgaccgtt gccgactggc atacttactt
     3661 cgtcaagggc aatcaggcgg aaacggaagg ggtttgggtt cataattctt gtccgccata
     3721 taagagacca aataacgcaa cgacaaaagc gcaacgagaa agtgtacaag gaaaaccctg
     3781 cgttgaatgc ggtaaattag ccgaaaaaat gattgccgac cataaaaaac cattagttgt
     3841 cgagtattat gaaacaggaa caattgacaa atctaagatg agagcaattg aatcagtaca
     3901 accgcaatgt ccgacttgtt ctgcaaaaca aggagggaga ttaagccaat attcaaaaga
     3961 acagaaaagg aaactgagta atgggaataa aaaatgaaca aataataatt tgtaaaaaat
     4021 ataatacaga aatatatcct gtttccgatg tttcaaaaat cggtgttgct gaaaatgtca
     4081 aacaggcaaa cctgtatccg attaacgggt taaggcaccg tcccaaaggt gatactaacg
     4141 gttggtatat ctgggccggt gaaaacttct cccatgatga aaattttttc ctgcccctgc
     4201 atactttcca tttgcaaata tggcgacccg aaatcatccc ctttttgaca cttccgcccg
     4261 gttaccgttt tttaattggg gaaaacggtt atgaagatgt atggtttgat gagttgctat
     4321 taaatgataa ttgacgaaaa atctacttca gatgtactga aacaatatca agaactaggg
     4381 tttgatttga caaaacctat gataatagag ttttttatag gaggttctca aaaaaatctg
     4441 caatcaatag aaagccaact catttcttat cggcaagatt ttcaaatcag tattgagcaa
     4501 aatgaattcg gagaaatgtg gacttgctat tgttccgtca atattatccc ttctcttgaa
     4561 aacattttag ctattgaaac tacgttattt ggtattgcac aaaaaaatga ttgtaattat
     4621 gaaggttttg gttcgtatgg gaactaacaa gaagtgtagg ttgggttgaa aaacacaaca
     4681 atcaaaaggt cgtctgaaac catgtttaat tttcagatgg cttttccttc atttaaaaca
     4741 ggatattgag atctaagttc tttaaaaatc ctacacctgc tccttccacg gcagcacctt
     4801 ggtcaaaacg gcagacggct gaaaagcaaa caccgtccgt cgtgttgccg tttgcggatg
     4861 agtacgggtc aaccccaatg ccgccgaaac cgtcgaagcc gccttcaaca ttgccgccgc
     4921 caaagccgca aagttggcaa aaacggtaaa accggggaga taaaagccga tggcaggaaa
     4981 gtaaatgtga ggatagacag tacggaggca gacctgcttt atccggcagg gcaataagaa
     5041 aacaaaaatt agatatggaa aacgattgtg taaaccatta caattttcta acaataatca
     5101 cagattttat gtggacaaaa tttgtgttac cgaacaagat gtgaacattt tggcatccga
     5161 ccgaaattat gaattaaata ttgaaatatt tattgacgat ataattcatt ttcaaataac
     5221 agatgaatct tataaagtaa aattttcaga atatttattc gaaa
//
LOCUS       DAWHSK010000129         5264 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.129, whole genome shotgun sequence.
ACCESSION   DAWHSK010000129 DAWHSK010000000
VERSION     DAWHSK010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5264
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.129"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(130..1047)
                     /gene="thrB"
                     /locus_tag="WIC03_001403"
     CDS             complement(130..1047)
                     /gene="thrB"
                     /locus_tag="WIC03_001403"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEZ3883887.1"
                     /translation="MSVYTSVSDDEMRGFLSGYDLGEFVSLQGIAQGITNSNYFLTTT
                     SGRYVLTVFEVLKQEELPFFLELNRHLSMKGVAVAAPVARKDGRLDSVLAGKPACLVA
                     CLKGSDTALPTAEQCFHTGAMLAKMHLAAADFPLEMENPRYDAWWTEACARLLPVLPR
                     DDAALLCSEIDALKDNLGNHLPSGIIHADLFKDNVLLDGGQVSGFIDFYYACRGNFMY
                     DLAIAVNDWVRTADNKLDEALKKAFIGGYEGVRPLSAEEKAYFPTAQRAGCIRFWVSR
                     LLDFHFPQAGEMTFIKDPNAFRNLLLSLD"
     gene            complement(1084..1812)
                     /gene="ubiG"
                     /locus_tag="WIC03_001404"
     CDS             complement(1084..1812)
                     /gene="ubiG"
                     /locus_tag="WIC03_001404"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HEZ3883888.1"
                     /translation="MGGKMSDKKYNVDEGEIAKFSRIADKWWDKSGEFKTLHDINPLR
                     LDYIDGHADLRGKRVLDVGCGGGILAESMARRGAAFVKGIDMAEQSLETARLHAALNN
                     VADIEYECIRVEDLAEAEPHSFDVVTCMEMMEHVPDPAAIVRACAKLVKPDGMVFFST
                     INKNPKSYLHLIVAAEYLLKFVPKGTHDWKKFIAPAELARMCRQAGLDVADTKGMTYH
                     VLSQTYALCDSTDVNYMFACRPAF"
     gene            1964..3205
                     /locus_tag="WIC03_001405"
     CDS             1964..3205
                     /locus_tag="WIC03_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235268.1"
                     /note="tryptophan transporter of high affinity; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HEZ3883889.1"
                     /translation="MPNKTPSLFGGAMIIAGTVIGAGMLANPTATSGVWFAGSLAVLL
                     YTWFSMLSSGLMILEVNTHYPHGASFDTMVKDLLGRGWNIINGIAVAFVLYLLTYAYI
                     FVGGDLTAKGIGNALGGDVSLTVGQLVFFGILAFCVWASARLVDRFTSVLIGGMVLTF
                     IWATGGLIADAKLPVLFDTQAPTGTNYWIYAATALPVCLASFGFHGNVSSLLKYFKGD
                     APKVAKSIWTGTLIALVIYVLWQTAIQGNLPRNEFAPVIAAEGQVSVLIETLSKFAQT
                     GNMDKILTLFSYMAIATSFLGVTLGLFDYIADIFKWNDSVSGRTKTAALTFLPPLVSC
                     LLFPTGFVTAIGYVGLAATVWTGIIPAMLLYRSRKKFGAGKTYKVYGGLWLMVWVFLF
                     GIVNIAAQVLSQMELVPVFKG"
     gene            3338..3901
                     /gene="gmhB"
                     /locus_tag="WIC03_001406"
     CDS             3338..3901
                     /gene="gmhB"
                     /locus_tag="WIC03_001406"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HEZ3883890.1"
                     /translation="MKLIILDRDGVINQDRDDFVKSVDEWIPVEGSMDAVAFLTQAGY
                     TVAVATNQSGIGRKYFTVQNLTEMHAKMHRLVRQAGGEINGIWFCPHTDADNCNCRKP
                     KPGMIEDIIGRFNAQASETWLVGDSLRDLQAIDAVGGKPALVLTGKGKKTLSQHGHEL
                     PEHTQVFDTLLDFSQYIMQENAAPQAD"
     gene            3931..4674
                     /locus_tag="WIC03_001407"
     CDS             3931..4674
                     /locus_tag="WIC03_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEZ3883891.1"
                     /translation="MLIIRNLIYWLILCSTLIFLFPFMLLASPFRDGAHKMARVWVKI
                     LNLSLKHIVGLKYRIIGAENIPDRPAVICAKHQSGWETLALQDIFPPQVYVAKRELFK
                     IPFFGWGLKLVKTIGIDRNNRREANEQLIKQGLARKNEGYWITIFPEGTRLAPGKRGK
                     YKLGGARMAKMFEMDIVPVALNSGEFWPKNSFLKYPGEITVVICPTIPHASGSEAELM
                     EKCEHLIETQQPLISGAGPFAAKMPSETV"
     gene            4671..>5264
                     /locus_tag="WIC03_001408"
     CDS             4671..>5264
                     /locus_tag="WIC03_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEZ3883892.1"
                     /translation="MTSLIHTLSDGMELTIEIKRRAKKNLIIRPIGTHTVRISVPPCF
                     SVSALNRWLYENEAVLRRTLAKTPPQTAENRLPESILFHGRQLALTAHQDTQILLMPS
                     EIRVPEGAPEKQLALLQDFLERQAHSYLIPRLERHARTTQLFPASSSLTSAKTFWGVC
                     RKTTGIRLNRRLVGAPEYVADYVCIHELCHLAHPDHSP"
BASE COUNT         1239 a         1717 c         1226 g         1082 t
ORIGIN      
        1 gtcctgattt ttgttaatcc actataaaat ggaaaatact cggctatcgt ctcattttcg
       61 ttttaatcag ccataaaaat gccgtctgaa accctttcag acggcatttc tgtcaaacgc
      121 cggacgcact caatccaaac tcaacagcag gttgcggaac gcgttcgggt ctttgataaa
      181 cgtcatctcg cccgcctgcg gaaaatgaaa gtccaacagg cgcgacaccc aaaaacggat
      241 gcagccggca cgttgggcgg tcgggaaata cgccttttct tcggcactca aggggcgcac
      301 gccctcataa ccgccgataa acgccttttt caacgcctca tccaacttat tgtccgccgt
      361 ccttacccaa tcgttgaccg caatcgccaa gtcatacata aaattgcccc ggcaggcata
      421 atagaaatcg atgaaacccg atacctgacc gccgtcaagc aacacattat ccttgaacaa
      481 atcggcgtgg atgatgcccg aaggcagatg gttgccgaga ttgtccttca gcgcatcgat
      541 ttcggaacac agcagcgcgg catcgtctcg cggcaggacg ggcagcagtc gggcgcacgc
      601 ctctgtccac cacgcatcat aacgcgggtt ttccatttcc aaagggaaat cggcggcggc
      661 aaggtgcatt ttcgccaaca tcgcgccggt atggaaacac tgttccgccg tcggcagcgc
      721 ggtgtccgaa cctttcaggc aggcaaccag gcatgcgggc ttgcccgcca aaacggaatc
      781 aagccggccg tctttgcgcg caaccggcgc ggcaaccgcc acgcccttca tactcaaatg
      841 ccggttaagc tccagaaaaa acggcagctc ttcctgtttc aacacttcaa acacggtcag
      901 cacataacgt cccgaagtcg tcgtcagaaa ataattgctg ttggtaatcc cctgcgcgat
      961 gccctgcagg gaaacaaatt cccccaaatc gtaaccgctc aggaagccgc gcatttcatc
     1021 atcggaaaca ctggtataga cagacatatc aactcacttg ctcaaaaacg ggcaaacccg
     1081 ccgtcagaac gccggacggc aggcaaacat ataattcaca tcggtcgaat cgcacagggc
     1141 ataagtttgc gacaacacat ggtaagtcat acccttcgta tccgccacat ccaagcctgc
     1201 ctgacggcac atccgcgcca gctcggcagg cgcgatgaat tttttccagt cgtgcgtgcc
     1261 tttggggaca aacttcaaca gatattccgc cgccacaatc agatgcaggt acgatttcgg
     1321 gtttttattg atggtggaaa aaaacaccat gccgtccggt ttgaccagct tggcacaagc
     1381 acgcacgatg gcggcgggat cggggacgtg ttccatcatt tccatgcacg ttaccacatc
     1441 gaacgagtgc ggttccgcct cggcaaggtc ttccacgcgg atacattcgt attcgatatc
     1501 ggcgacattg ttcaaagccg cgtgcaggcg ggcggtttcc agcgactgct ccgccatgtc
     1561 gatacccttt acaaacgccg cgccgcgccg cgccatactt tccgccaaga tgccgccgcc
     1621 gcagcccacg tccaaaaccc gtttgccgcg caaatccgcg tgtccgtcga tataatccag
     1681 ccgcagcgga ttgatgtcgt gcaaggtttt gaactcgccc gacttgtccc accatttgtc
     1741 ggcaatccgg ctgaatttgg cgatttcccc ctcatcgaca ttatattttt tgtcggacat
     1801 tttccctccc atctgacgaa ccgcccactc caaaacccaa gattatacta tggaacacaa
     1861 tgccgtctga aagccttttc gggcggcgcg gcgcaaatgc cttacaaatc cttacacttt
     1921 acggcataat ggcggctcgc tttttctggc agaaagacaa aatatgccca acaaaacccc
     1981 ttcactgttc ggcggcgcga tgattatcgc cggcacggtc atcggcgcag gcatgctcgc
     2041 caacccgacc gccacatccg gcgtatggtt tgccggctcg ctggccgtgt tgctgtacac
     2101 ctggttttct atgctttcca gcggtctgat gattttggaa gtcaacaccc actaccccca
     2161 cggcgcaagt ttcgacacga tggtcaaaga cctgctcgga cgcggctgga acatcatcaa
     2221 cggcatcgcc gtcgccttcg ttttatacct gctcacctac gcctacatct tcgttggcgg
     2281 cgacctgacc gccaaaggca tcggcaacgc cttgggcggc gacgtttcac tcaccgtcgg
     2341 acaactcgtc ttcttcggca ttctcgcctt ttgcgtatgg gcatccgcac gcttggtcga
     2401 ccgattcacc agcgtcctca tcggcggcat ggtattaacc tttatttggg caaccggcgg
     2461 cctgattgcc gatgccaaac tgcccgtcct cttcgacacc caagccccta ccggcacaaa
     2521 ctactggatt tacgccgcca ccgccctgcc cgtctgcctc gcttccttcg gcttccacgg
     2581 caacgtctcc agcctgctca aatactttaa aggcgacgcg cccaaagtgg cgaaatccat
     2641 ctggacgggc acactgattg cgctggtgat ttacgtcctc tggcaaaccg ccatccaagg
     2701 caacctgccg cgcaacgagt tcgcccccgt catcgccgcc gaagggcaag tctccgtcct
     2761 catcgaaacc ctgtccaaat tcgcccaaac cggcaatatg gataaaatat tgaccctgtt
     2821 ttcctatatg gcgatcgcca cctcgttttt aggcgtaacg ctcggactct tcgactacat
     2881 cgccgacatc ttcaaatgga acgacagcgt gtccggccgt accaaaaccg ccgcgctgac
     2941 cttcctgccg cccctggttt cctgcctgct cttccccacc ggcttcgtta ccgccatcgg
     3001 ctacgtcggc ctggcggcaa ccgtctggac aggcatcatc cccgccatgc tgctctaccg
     3061 ttcgcgcaaa aaattcggcg caggcaaaac ctataaagtt tacggcggct tgtggctgat
     3121 ggtttgggtc ttccttttcg gcatcgtcaa catcgccgca caggtattga gccaaatgga
     3181 actcgtcccc gtatttaaag gataaaagca aaatgccgtt tgaagcccgc cggcggcttc
     3241 aaacggcatt gcctcgttat cacgcggcac aggcgcatca ctgccgatat ttcagtataa
     3301 tgccaccccc gacctgcccc aatccaaagg aaacgcgatg aaactcatca ttctcgaccg
     3361 cgacggcgtc atcaatcagg accgcgatga cttcgtcaaa tccgttgacg agtggatacc
     3421 tgtcgaaggc agcatggatg cggtggcatt tctgacgcag gcaggctaca ccgtcgccgt
     3481 tgccaccaac caatccggca tcgggcgcaa atattttacc gttcaaaacc ttaccgaaat
     3541 gcacgccaaa atgcaccgcc tcgtccgtca ggcaggcggc gaaatcaacg gcatctggtt
     3601 ctgcccgcac accgatgccg acaactgcaa ctgccgcaag cccaaaccgg gtatgattga
     3661 agacatcatc ggacgcttca acgcccaagc ctcggaaacc tggctggtcg gcgacagcct
     3721 gcgcgatttg caggcaatcg atgccgtcgg cggaaaaccc gcgctggttc tgaccggaaa
     3781 aggcaaaaaa acgctctccc aacacggaca cgaattaccc gaacacacac aggttttcga
     3841 taccttgctc gatttctcac aatacatcat gcaggaaaac gccgcaccgc aagccgactg
     3901 aacataccgc atttccgaca aggcaaaacc atgctcatca tccgcaacct gatttactgg
     3961 ctgatactct gttccaccct gattttcctc tttcccttta tgctgctcgc ctcgcctttc
     4021 cgggacgggg cgcacaagat ggcgcgggtc tgggtcaaaa tcctcaacct ctcgctcaaa
     4081 cacatcgtcg ggctcaaata ccgcatcatc ggcgcggaaa acatccccga ccgccccgcc
     4141 gtcatctgcg ccaaacacca aagcggctgg gaaacgcttg cccttcagga catttttccg
     4201 ccgcaggttt acgttgccaa acgcgagctg ttcaaaatcc cctttttcgg ctggggttta
     4261 aaactggtca aaaccatagg catagaccgc aacaaccgcc gcgaagccaa cgaacagctc
     4321 ataaaacagg ggttggcgcg caaaaacgaa ggctattgga ttaccatttt ccccgaaggc
     4381 acgcgccttg cgcccggaaa acgcggcaaa tacaaactcg gcggcgcgcg catggcgaaa
     4441 atgtttgaga tggacatcgt ccccgtcgcc ctcaacagcg gcgaattttg gccgaaaaac
     4501 tcctttctga aatatccggg ggaaatcacc gtcgtcatct gtccgaccat cccgcacgca
     4561 agcggcagcg aagccgaatt gatggaaaaa tgcgaacacc tcatcgaaac gcagcagccg
     4621 ctcatttccg gcgcaggccc gtttgccgcc aaaatgccgt ctgaaaccgt atgacctccc
     4681 tgatccacac tctttctgac ggcatggaac tgactataga aatcaagcgc cgtgccaaga
     4741 aaaacctgat tatccgccct atcggcacac ataccgtccg catcagcgtc ccaccctgct
     4801 tctccgtctc cgctctaaac cgctggctgt atgaaaacga agccgtcctg cggcgaacac
     4861 tggcgaaaac accgccgcaa actgccgaaa accggctgcc cgaatccatc ctcttccacg
     4921 gcagacaact tgccctcacc gcccatcaag acacgcaaat cctgctgatg ccgtctgaaa
     4981 tccgcgttcc cgaaggcgca cccgaaaaac agcttgcgct gctgcaggac tttttggaac
     5041 ggcaggcgca cagttacctg attccccgcc tcgaacgcca cgcccgcacc acacaactgt
     5101 tccccgcctc ctcctcgctg acctctgcca aaaccttctg gggcgtgtgc cgcaaaacca
     5161 caggcatacg cctcaaccgg cggctggtcg gcgcaccgga atacgttgcc gattatgtct
     5221 gcatacacga actctgccac ctcgcccatc ccgaccacag cccc
//
LOCUS       DAWHSK010000130         5259 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.130, whole genome shotgun sequence.
ACCESSION   DAWHSK010000130 DAWHSK010000000
VERSION     DAWHSK010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5259
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.130"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(235..1590)
                     /locus_tag="WIC03_001409"
     CDS             complement(235..1590)
                     /locus_tag="WIC03_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HEZ3883893.1"
                     /translation="MSIQSGEILETVKMVADQNFDVRTITIGIDLHDCISSDINVLNQ
                     NIYNKITTVGKDLVTTAKYLSAKYGVPIVNQRISVTPIAQIAAATHADSYVSVAQTLD
                     KAAKAIGVSFIGGFSALVQKGMSPSDEVLIRSIPEAMKTTDIVCSSINIGSTRAGINM
                     DAVKLAGETVKRTAEITPEGFGCAKIVVFCNAVEDNPFMAGAFHGSGEADAVINVGVS
                     GPGVVKAALENSDATTLTEVAEVVKKTAFKITRVGELIGREASKMLNIPFGILDLSLA
                     PTPAVGDSVARILEEMGLSVCGTHGTTAALALLNDAVKKGGMMASSAVGGLSGAFIPV
                     SEDEGMIAAAEAGVLTLDKLEAMTAVCSVGLDMIAVPGDTPAHTISGIIADEAAIGMI
                     NSKTTAVRIIPVTGKTVGDSVEFGGLLGYAPVMPVKEGSCEVFVNRGGRIPAPVQSMK
                     N"
     gene            complement(1605..1877)
                     /locus_tag="WIC03_001410"
     CDS             complement(1605..1877)
                     /locus_tag="WIC03_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEZ3883894.1"
                     /translation="MNNSVITVIGKDRVGIVYDVSKILAENQINILNISQQLMDDFFT
                     MIILVDTSKCSKSRQEVLDLFAEESKKLALDIRMQNEEIFQAMHRI"
     gene            complement(2003..2740)
                     /locus_tag="WIC03_001411"
     CDS             complement(2003..2740)
                     /locus_tag="WIC03_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HEZ3883895.1"
                     /translation="MKKLSRIVFSTVLLGFSAALPAQTYSVYFNQNGKLTATMSSAAY
                     IRQYSVVAGIAHAQDFYYPSMKKYSEPYIVASTQIKSFVPTLQNGMLILWHFNGQKKM
                     AGGFSKGKPDGEWVNWYPNGKKSAVMPYKNGLSEGTGYRYYRNGGKESEIQFKQNKAN
                     GVWKQWYADGSIKTEMVMVNDEPAKILTWDESGRLLSELSIRHHQRNGVVLEWYEDGS
                     KKSEAVYQDDKLVRKTQWDKDGYLIEP"
     gene            complement(3021..3920)
                     /gene="prmB"
                     /locus_tag="WIC03_001412"
     CDS             complement(3021..3920)
                     /gene="prmB"
                     /locus_tag="WIC03_001412"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEZ3883896.1"
                     /translation="MFNQAAQELTTIRDILRFAVSRFNEAGLFFGHGTDNAHDEAAYL
                     ILHTLNLPLDMLAPYLDAKLLEAEKEEVLAVIERRAVEHIPAAYLTHQAWQGEFDFYV
                     DERVIIPRSFIYELLGDGLRPWIEYDELVHNALDLCTGSGCLAIQMAHHYPDAQIDAV
                     DVSLDALEVAGINVEDYGLEERIRLIHTDLFEGLEGTYDLIVSNPPYVDAESVELLPE
                     EYLHEPELALGSGADGLDATRQILLNAAKFLNPKGVLLVEIGHNRDVLEAAYPELPFT
                     WLETSGGDGFVFLLTREQLLGEE"
     gene            4065..4658
                     /locus_tag="WIC03_001413"
     CDS             4065..4658
                     /locus_tag="WIC03_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883897.1"
                     /translation="MADGMGNLIGWEKTGLVVGKQWITAKDDKVSDVCNANGEMGVIG
                     LYEPFSHGALTIPGHPNCRCEVVSVSGGELGEFAEKKELRKAAMQYARDNFIGKSYVN
                     KNSGHELKVTWQGVKHAASKANQAELSIMTKLDDLLRYAKYEGSYSDRKGHPNIIAAH
                     KYRAVAKVGNESLNIGVIVREFPDDHKHYDHFILKDE"
     gene            4770..>5259
                     /locus_tag="WIC03_001414"
     CDS             4770..>5259
                     /locus_tag="WIC03_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HEZ3883898.1"
                     /translation="MTKLYAEIAKMETQDDDTVKVWGYASSEEIDSDGEVIAAAAMKA
                     AIPDYMKFGAGREMHGSNAAGTAIEINVEDDGITFFGAHIIDPVVVSKVKTGVYKGFS
                     IGGSVTARDDLNKSQITGLKLTEISLVERPANPDAVSTCFKADKGAEAVNNDTEHNAT
                     YFS"
BASE COUNT         1460 a         1322 c         1164 g         1313 t
ORIGIN      
        1 attcccactt tcgcggggat gacggcggat aggttttgtt gtttcggata aattcctgtt
       61 gttttttggt ttccagattc ccacctttaa aagaatgacg taagaaagca gaaataagga
      121 cacagggatt ttttaaattt gcaatattct tttctaaaga cattttaccc gatgcctata
      181 aacaaaaatg ccgtctgaag cctttcagac ggcatttccc catcaaaacc gcaatcagtt
      241 tttcatcgat tgaaccggtg cgggaatcct gccgccccgg ttgacgaaca cttcgcatga
      301 gccttctttt accggcatta caggcgcgta gcccaacagg ccgccgaact cgacgctgtc
      361 gccgacggtt ttaccggtta ccggaataat gcgcacggca gtggttttgc tgttgatcat
      421 gccgatggcg gcttcgtcgg caatgatgcc ggaaatggtg tgcgcgggtg tgtcgccggg
      481 aacggcaatc atatccaagc cgaccgaaca aacggcggtc atggcttcga gtttgtccag
      541 cgtcagcacg cctgcttcgg cggcggcaat cataccttcg tcttcggaaa cggggataaa
      601 cgcgccactc aaacccccga ccgcgctgga agccatcatg ccgccttttt tcacggcatc
      661 gttcagcaat gccaaagctg ctgttgtgcc gtgcgtaccg cagacgctca agcccatttc
      721 ttcaagaatg cgtgccactg agtcgccgac ggcgggggtc ggcgccagcg acaagtcgag
      781 aataccaaac gggatattca gcatttttga ggcttcgcgg ccgatgagtt cgcccacgcg
      841 ggtaattttg aaagcagttt tcttcactac ttccgcaact tcggtcaatg tcgttgcatc
      901 tgaattttcc aacgcggctt ttacgacacc tgggccggat acgccgacat tgataacggc
      961 atccgcttcg cccgaaccat gaaacgcgcc cgccataaac gggttgtctt ccaccgcgtt
     1021 gcagaacacg acaattttag cgcagccgaa accttcgggc gtgatttccg ccgtgcgttt
     1081 gacggtttcg cccgccagct tgaccgcatc catattgata ccggcacgcg tactgccgat
     1141 attgatggag ctgcacacaa tatcggtagt cttcatcgct tcgggaatgg agcggattaa
     1201 cacctcatcc gaaggcgaca tccctttttg caccaacgcg gaaaaaccgc cgataaaaga
     1261 cacaccgatg gctttggcag ctttatccaa agtttgcgcc acgctgacgt aagaatcagc
     1321 atgggtggcc gccgcgattt gggcaatcgg cgtaacggaa atgcgctgat tcacaatcgg
     1381 tacgccgtat ttggcagaca gatattttgc cgtagtgacc aagtctttgc cgactgtggt
     1441 aattttattg taaatatttt ggttcaacac attgatatcg ctgctgatgc agtcgtgcaa
     1501 atcaatgccg atggtaatgg tgcggacatc aaaattctgg tcggcaacca ttttgacggt
     1561 ttctaaaatt tcgccggatt ggatactcat cacattcctc caactcaaat gcggtgcatc
     1621 gcttggaaga tttcttcgtt ttgcatacgg atatcaagcg cgagtttttt gctctcttcc
     1681 gcaaacaaat ccaaaacctc ttgacgcgat ttgctgcatt ttgaagtgtc caccaagata
     1741 atcatagtaa aaaaatcgtc catcagctgt tggctgatgt tgagaatatt gatttggttt
     1801 tccgccaaaa ttttggaaac atcgtacacg atgccgacgc ggtctttacc gatgacggtg
     1861 atgactgaat tgttcacagg cttactcctt gcagatatcc gttaaagtcc gaaattatac
     1921 caccgttgga ttttgaagaa atattgtcaa caatatatac atacaaaatg ccgtctgaaa
     1981 ctatttcaga cagcatcaag attcagggtt cgattaaata accatcctta tcccactggg
     2041 ttttcctgac caacttgtca tcctgataaa cagcttcgct ctttttagaa ccatcttcat
     2101 accactccaa aaccaccccg ttgcgttgat ggtggcggat agacagttcc gagagtaatc
     2161 ggccgctttc atcccaagtc agaattttgg caggctcatc gttgaccata accatttccg
     2221 tcttgatact gccgtcggca taccattgct tccatacgcc gtttgcctta ttttgcttaa
     2281 actggatttc gctttccttg ccgccgttac ggtaatagcg gtatcccgta ccctcactca
     2341 agccattttt ataaggcata acggcagatt ttttaccgtt cggataccag ttgacccact
     2401 ccccgtccgg cttacccttg ctgaagcccc ccgccatttt tttctgacca ttaaaatgcc
     2461 acaaaatcaa cataccgttt tgcagggtag gcacaaaaga tttgatttgc gttgaagcaa
     2521 cgatataagg ttcagaatat ttcttcatcg acggataata aaaatcctgc gcgtgcgcaa
     2581 tacccgccac cacactatat tgcctgatat aagcggcaga agacatcgtc gccgtcagct
     2641 ttccgttctg attaaaataa acagaatagg tctgcgccgg caaagcggcc gaaaaaccca
     2701 acaggacagt tgaaaataca atccgagata attttttcat tgcaatagcg atataaaaac
     2761 aaggctgtgt tttagtaatc tgttgatttc aattatttgc aagggaaaag acaattattt
     2821 tccggttagg aataaaccta ttctattgaa tatattgaag ccaagtacgc ctatcaacac
     2881 tatattaaaa cactgccaaa aacaattaac ttataaacaa tatggtaagg atttctctgc
     2941 caagcatcaa acccgagaca acgtatcgta aaaatgccgt ctgaaaacaa atcgtcttca
     3001 gacggcattt ccccttcaac tcactcttca cccaataact gctcgcgcgt caagaggaaa
     3061 acaaaaccgt cgcccccgct ggtttccaac caagtaaaag gcaactccgg atacgctgct
     3121 tccaatacat ccctgttatg cccgatttcc accagcaata cacctttggg attcagaaac
     3181 tttgccgcat tcagaagaat ctgcctggtg gcatccaacc cgtccgcccc gctgcccaat
     3241 gccaattccg gttcgtgcaa atactcttca ggcaataact caaccgattc cgcatccaca
     3301 taaggaggat tggaaacaat caaatcataa gtgccttcca atccttcaaa caaatccgta
     3361 tgaataagcc ggatgcgttc ttccaaacca taatcttcga cattaatccc tgccacttcc
     3421 aaagcatcca agctcacatc aaccgcatca atttgggcat caggataatg atgcgccatc
     3481 tgaatggcaa ggcaaccgct tccggtgcaa agatccaaag cattatgcac caactcatcg
     3541 tattctatcc aaggacgaag tccgtcaccc aacaattcat aaataaaaga acgaggtatg
     3601 attacgcgct catccacata gaaatcaaac tctccctgcc atgcctggtg tgtcaaataa
     3661 gcggctggaa tgtgttcgac agcacgacgc tcaataaccg ccagcacttc ctctttttca
     3721 gcttccaaga gttttgcatc aagatatggg gcaagcatat ccaaaggcaa attcaaagta
     3781 tgcagaatca aataagctgc ttcatcatgc gcattatctg ttccatgacc aaaaaagagc
     3841 cctgcctcat taaaacggct gactgcaaaa cgtaaaatat cgcggatagt cgtcaattct
     3901 tgtgctgcct gattaaacat aatatgaacc attctgcgta tagatacttt taattataac
     3961 agaaacaaca agcaaacctt ttcatatcgc caaataacca cccaatctac ccatacaact
     4021 acataaatgc ccgcgcgaaa accatcgccc gaacggaaac gacaatggcc gacggtatgg
     4081 gcaatctgat tggctgggaa aaaacggggc ttgttgtcgg taagcagtgg ataaccgcaa
     4141 aagacgacaa ggtgtccgat gtctgcaatg ccaacggcga gatgggcgta atcgggcttt
     4201 acgagccttt ctcacacggc gcattgacga tacccggtca tccgaactgc cgatgcgagg
     4261 ttgtttccgt atcgggtggc gaattggggg aatttgccga aaaaaaggag cttcgtaaag
     4321 cggctatgca gtatgcgcgg gataacttta tcggcaaaag ctatgtcaat aaaaacagcg
     4381 ggcatgaact gaaggtaact tggcaaggtg tgaaacacgc tgcgtcaaag gcaaatcagg
     4441 cggaattatc catcatgaca aaacttgatg acttattgcg ctacgcaaaa tatgagggtt
     4501 cttattcgga taggaaaggt catcctaata ttattgcagc acataagtat cgtgccgttg
     4561 ccaaggttgg gaatgagtct ttaaatatcg gtgtgattgt aagggaattt ccagacgacc
     4621 ataaacatta cgaccatttc atcttgaagg atgaataaag cccttttgca gtgtcgttct
     4681 ggagcggata gcgttaaggc aagtacactt ccagccttga aaaagggctt taaattcagc
     4741 atgccattta tacaggcagg agtaaaccca tgacaaagtt atacgcagaa atcgccaaga
     4801 tggagacgca ggacgacgac acggtcaagg tttggggtta cgcttcaagc gaggaaatcg
     4861 attcggacgg cgaagtcatc gcggcggcag ctatgaaggc ggcgattccc gattatatga
     4921 agtttggcgc ggggcgcgag atgcacggct caaacgctgc gggaacggca attgaaatca
     4981 acgttgaaga cgacggcata acctttttcg gtgctcatat catcgacccc gttgtcgtgt
     5041 cgaaagtcaa aacaggcgtt tacaaaggct tttccatcgg cggcagcgtt accgcccgcg
     5101 atgatttgaa caagtcgcaa atcacgggct tgaagctgac ggaaatcagc cttgtcgagc
     5161 gccctgccaa tcccgatgcg gtgtctacct gctttaaggc ggacaaaggt gcggaagcgg
     5221 taaacaacga tacagaacat aatgctacat attttagcc
//
LOCUS       DAWHSK010000131         5224 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.131, whole genome shotgun sequence.
ACCESSION   DAWHSK010000131 DAWHSK010000000
VERSION     DAWHSK010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5224
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.131"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            191..406
                     /locus_tag="WIC03_001415"
                     /pseudo
     CDS             191..406
                     /locus_tag="WIC03_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003792345.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(558..1616)
                     /gene="trpD"
                     /locus_tag="WIC03_001416"
     CDS             complement(558..1616)
                     /gene="trpD"
                     /locus_tag="WIC03_001416"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEZ3883899.1"
                     /translation="MITPQQAIERLISNNELFYDEMTDLMRQIMSGKVPPEQIAAILT
                     GLRIKVETVSEITAAAAVMREFATKVPLENAEGLVDIVGTGGDGAKTFNISTTSMFVA
                     AAAGAKVAKHGGRSVSSSSGAADVVEQMGANLNLTPEQVAQSIRQTGIGFMFAPNHHS
                     AMRHVAPVRRSLGFRSIFNILGPLTNPAGAPNQLLGVFHTDLCGILSRVLQQLGSKHV
                     LVVCGEGGLDEITLTGKTRVAELKDGKISEYDIRPEDFGIETRRNLDEIKVANTQESL
                     LKMNEVLEGREGAARDIVLLNTAAALYAGNVAASLSDGISAAREAIDSGRAKSKKEEF
                     VGFQPQQRCHFLGKMELG"
     gene            complement(1679..2269)
                     /locus_tag="WIC03_001417"
     CDS             complement(1679..2269)
                     /locus_tag="WIC03_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEZ3883900.1"
                     /translation="MLLFIDNYDSFTYNIVQYFAELGQEVAVRRNDDITLEEIEALNP
                     QYLVIGPGPCSPKEAGISVAAMRHFAGRLPIMGVCLGHQTIGEAFGGRIVRAKTLMHG
                     KVSPVSHSGKGMFMGLPNPVTCTRYHSLVIERGTLPECLEITAWTEDGEIMGVRHKEY
                     AVEGVQFHPEALLTEHGHDMLNNFLIEFQNFKPQKI"
     gene            2691..4967
                     /locus_tag="WIC03_001418"
     CDS             2691..4967
                     /locus_tag="WIC03_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ3883901.1"
                     /translation="MTQTTLKPIVLSILLISTPILSQAHETEQSVDLETVSVVGKSRP
                     RATSGLLHTSTASDKIISGDTLRQKAVNLGDALDGVPGIHASQYGGGASAPVIRGQTG
                     RRIKVLNHHGETGDMADFSPDHAIMVDTALSQQVEILRGPVTLLYSSGNVAGLVDVAD
                     GKIPEKMPENGVSGELGLRLSSGNLEKLTSGGINIGLGKNFVLHTEGLYRKSGDYAVP
                     RYRNLKRLPDSHADSQTGSIGLSWVGEKGFIGVAYSDRRDQYGLPAHSHEYDDCHADI
                     IWQKSLINKRYLQLYPHLLTEEDVDYDNPGLSCGFHDDDDAHAHAHNGKPWIDLRNKR
                     YELRAEWKQPFPGFEALRVHLNRNDYHHDEKAGDAVENFFNNQTQNARIELRHQPIGR
                     LKGSWGVQYLGQKSSALSAIPETVQQPMLLDNKVQHYSFFGVEQANWDNFTLEGGVRV
                     EKQKASIRYDKALIDRENYYNHPLPDLGAHRQTARSFALSGNWYFTPQHKLSLTASHQ
                     ERLPSTQELYAHGKHVATNTFEVGNKHLNKERSNNIELALGYEGDRWQYNLALYRNRF
                     GNYIYAQTLNDGRGPKSIEDDSEMKLVRYNQSGADFYGAEGEIYFKPTPRYRIGVSGD
                     YVRGRLKNLPSLPGREDAYGNRPFIAQDDQNAPRVPAARLGFHLKASLTDRIDANLDY
                     YRVFAQNKLARYETRTPGHHMLNLGANYRRNTRYGEWNWYVKADNLLNQSVYAHSSFL
                     SDTPQMGRSFTGGVNVKF"
BASE COUNT         1328 a         1484 c         1211 g         1201 t
ORIGIN      
        1 tttgaagcgg ttgccggctt cctgccgtcc gctggcgcgt ccgtcatcac gccggcaacc
       61 gcctttgtca tcccttgctt atcttccatg gtgcgaatcc tcaaaaacgg gcaaaaaaaa
      121 gccctgttac ttgtagaaag taaaggacgt taatttttgt taatcgtccc ttcttaggga
      181 cgcaatatat aaggtttata ccgtcgttgt tcctaatgcg caatcagcga cattgttgcc
      241 aattatccga aagaaagtta agcctgatgg cattgtatac acggatacct ttcgtagtta
      301 tgatgtgctt gatattagtg aatttagcca tttacgtaag tttaacggca ttcccaaaga
      361 gcatttgggg ctgcatttaa agaaatgcca atggcatttt aaatagaatc tcaaattcag
      421 attttaagac aattagttaa tgggaatttg gtctggttat ctagtacagc cccaagtttt
      481 tatataaaaa cagtttggta agttctcttc gtgaagtgct tatgttttgc cacaaaagtt
      541 ccataatcat ggcggcatca cccaagctcc atctttccaa gaaaatggca tctttgttgt
      601 ggttgaaaac cgacaaactc ctcttttttc gatttggccc tgcctgaatc gatggcttcc
      661 cgtgcggcag atatgccgtc tgaaagcgaa gcagcgacat ttccggcata cagggcggcg
      721 gcggtgttga gcaatacgat atcgcgcgca gccccttctc ttccttccag cacctcattc
      781 attttcaaca aagattcctg agtattggca actttgattt catccaaatt gcggcgggtt
      841 tcgataccga aatcttctgg gcggatgtcg tattcgctga tttttccgtc tttgagctcg
      901 gcaacgcgtg ttttgcccgt cagtgtaatt tcatccaaac cgccctcccc gcaaacaacc
      961 aaaacatgct tggagccgag ctgttgcaat actctggaaa ggatgccgca caaatcggtg
     1021 tggaacacgc ccaaaagctg gttcggcgcg cccgcaggat tcgttaacgg acccaatatg
     1081 ttgaaaatac ttcggaaacc gagcgaacgg cgtacagggg cgacatggcg catggcactg
     1141 tggtgattgg gggcgaacat aaaccctata ccggtctgcc tgatactttg ggcaacctgt
     1201 tcgggagtca ggttgaggtt tgcccccatc tgctccacca cgtccgccgc gccgctggag
     1261 gaagagaccg accggcctcc gtgtttggca accttcgcgc ctgccgctgc ggcaacaaac
     1321 atcgaagtcg tcgaaatatt gaaggttttc gcgccatccc cgcccgtacc gacgatatcg
     1381 accagcccct ctgcattctc cagcggcact tttgtcgcaa actcgcgcat cacggctgca
     1441 gctgcggtaa tttcggaaac ggtttcaacc ttgatacgca agccggtcaa aatcgctgca
     1501 atttgttcgg gcggtacttt tccgctcata atctgacgca tcaagtcggt catttcatcg
     1561 taaaacaact cgttattgct gattaatcgt tcgatagcct gttgcggtgt aatcattttt
     1621 gtcctccgtt caatattcgg acgaaaatgc cgtctgaagg gcttcagacg gcatcgcgtc
     1681 agattttttg cggtttgaag ttttgaaatt cgattaaaaa attgttcaac atatcatgtc
     1741 cgtgttcggt caagagggct tcggggtgga actgcacgcc ctcgacggca tattccttat
     1801 ggcgcacgcc cataatctcg ccgtcttccg tccatgccgt gatttccaaa cattcgggca
     1861 gtgtgccgcg ttcgataacg aggctgtgat aacgcgtaca ggtaaccgga ttgggcaacc
     1921 ccataaacat acccttgccc gaatgggaca cgggcgacac cttgccgtgc atcagcgttt
     1981 tggcgcggac tatcctgccg ccgaacgcct cgcctatcgt ctgatgcccg aggcacacgc
     2041 ccataatcgg cagccggccg gcgaaatggc gcatcgccgc cacggaaatc cccgcttctt
     2101 tgggcgaaca cggaccgggg ccgataacga gatattgcgg attcaatgcc tcgatttcct
     2161 ccaacgtaat atcatcgttg cggcgcacgg caacttcctg ccccaattca gcgaaatact
     2221 ggacgatgtt gtaagtaaaa ctgtcgtaat tgtcgataaa caaaagcatt ttgtgattaa
     2281 cctattgatt tataatttgt ttgttattaa ctgcatttcc gataccccca tccataccct
     2341 cttatatctt agcgtgcccg atgcgccctc gtgaacctgg cgcagagcct cgcgttctga
     2401 caagtacgcc attttgccac cttttcgacc gttcgggaac gtgccgtttt tatgcactca
     2461 taaattttgg ttatcccgca gccgaacata atgggcaaac acagcggaat ctgcgtcaac
     2521 atcaaccgcg cccccgaagg acggcggata caaatggaca ccgcccgatg ccgcaaccgt
     2581 ttcatttgaa aaacttttgc ctatgccctc tgagtgttct caaaatctta tgatataaaa
     2641 aatgtaatgt tatataataa caaacttttg aaatgcgacg agaagccaaa atgacacaaa
     2701 ccacactcaa gcccattgtt ttatctattc ttttaatcag cacccctatt ctctcccaag
     2761 cgcatgaaac cgagcagtcg gtggacttgg aaacggtaag cgtcgtcggc aaaagccgtc
     2821 cgcgcgccac atccgggctg ttgcacactt cgaccgcctc cgacaaaatc atcagcggcg
     2881 acaccttgcg acaaaaagcc gtcaacttgg gcgatgcttt ggacggcgtt ccgggcattc
     2941 acgcttcgca atacggcggc ggcgcgtccg ctcccgttat tcgcggtcaa acaggcaggc
     3001 ggattaaagt gttgaaccat cacggcgaaa cgggcgatat ggcggatttt tcgcccgatc
     3061 acgccattat ggtagatacc gccttgtcgc aacaggtcga aatcctgcgc gggccggtta
     3121 cgctcttgta cagctcgggc aatgtggcgg ggctggtcga tgttgccgat ggcaaaatcc
     3181 ccgaaaaaat gcctgaaaac ggcgtatcgg gcgaactcgg attgcgtttg agcagcggca
     3241 atttggaaaa actcacgtcc ggcggcatca atatcggctt gggcaaaaac tttgtattgc
     3301 acacggaagg gctgtaccgc aaatcggggg attacgccgt accgcgttac cgcaatctga
     3361 aacgcctgcc cgacagccac gccgattcgc aaacgggcag catcgggctg tcttgggttg
     3421 gcgaaaaagg ttttatcggc gtagcgtaca gcgaccgtcg cgaccaatat ggtctgcctg
     3481 cccacagcca cgaatacgat gattgccacg ccgacatcat ctggcaaaag agcttgatta
     3541 acaaacgcta tttacagctt tatccgcacc tgttgaccga agaagacgtc gattacgaca
     3601 atccgggctt gagctgcggc ttccacgacg acgatgatgc acacgcccat gcccacaacg
     3661 gcaaaccttg gatagacctg cgcaacaaac gctacgaact ccgcgccgaa tggaagcagc
     3721 cattccccgg ttttgaagcc ctgcgcgtac acctgaaccg caacgactac caccacgacg
     3781 aaaaagcagg cgatgcagtc gaaaactttt ttaacaacca aacgcaaaac gcccgcatcg
     3841 agttgcgcca ccaacccata ggccgtctga aaggcagctg gggcgtgcaa tatttgggac
     3901 aaaaatccag cgcactctcc gccattcccg aaaccgtcca acaaccgatg ctgcttgaca
     3961 acaaagtgca acattacagc tttttcggtg tagaacaggc aaactgggac aacttcacgc
     4021 ttgaaggcgg cgtacgcgtg gaaaaacaaa aagcctccat ccggtacgac aaagcattga
     4081 ttgatcggga aaactactac aaccaccccc tgcccgacct cggcgcgcac cgccaaaccg
     4141 cccgctcgtt cgcactttcg ggcaactggt atttcacgcc acaacacaaa ctcagcctga
     4201 ccgcctccca tcaggaacgc ctgccgtcaa cgcaagagct gtacgcacac ggcaaacacg
     4261 tcgccaccaa cacctttgaa gtcggcaaca aacacctcaa caaagagcgt tccaacaaca
     4321 tcgaactcgc gctgggctac gaaggcgacc gctggcaata caatctggca ctctaccgca
     4381 accgcttcgg caactacatt tacgcccaaa ccttaaacga tggacgcggc cccaaatcca
     4441 tcgaagacga cagcgaaatg aagctcgtgc gctacaacca atccggtgcg gacttctacg
     4501 gtgcagaagg agaaatctac ttcaaaccga caccgcgcta ccgcatcggc gtttccggcg
     4561 actatgtacg aggccgtctg aaaaacctgc cgtccctacc cggcagggaa gatgcttacg
     4621 gcaaccgtcc tttcatcgca caggacgacc aaaatgcccc ccgtgttccg gctgcgcgcc
     4681 tcggcttcca cctgaaagcc tcgctgaccg accgtatcga tgccaatttg gactactacc
     4741 gcgtgttcgc ccaaaacaaa ctcgcccgct acgaaacgcg cacgcccgga caccatatgc
     4801 tcaacctcgg cgcaaactac cgccgcaata cgcgctatgg cgagtggaat tggtacgtca
     4861 aagccgacaa cctgctcaac caatccgttt acgcccacag cagcttcctc tctgatacgc
     4921 cgcaaatggg ccgcagcttt accggcggcg tgaacgtgaa gttttaaaat cggacaggca
     4981 aacaaaaaag gttttcagac ggcattctga aaaccttttt tctttttaat caatttgtta
     5041 ggaaatatgg caaagacaaa gaacaaacca ctatgccatc attcccattt tctttgtttc
     5101 atcattcttg ctttcacgcc gtcattctcg caaaagcaga aaaatcaaaa gcagaaaccc
     5161 atcattcccg cgcaggcgag aatccaattc attcggtttc agccgtttcc gataaatcgc
     5221 ctta
//
LOCUS       DAWHSK010000132         5209 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.132, whole genome shotgun sequence.
ACCESSION   DAWHSK010000132 DAWHSK010000000
VERSION     DAWHSK010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5209
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.132"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(102..2933)
                     /gene="lbpA"
                     /locus_tag="WIC03_001419"
                     /gene_synonym="tbpA"
     CDS             complement(102..2933)
                     /gene="lbpA"
                     /locus_tag="WIC03_001419"
                     /gene_synonym="tbpA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoferrin/transferrin-binding protein LbpA"
                     /protein_id="HEZ3883902.1"
                     /translation="MNRKHTFPLTLTALAVAAAFPSYAANPETATPDAAQTQSLKEVT
                     VRAAKVGRRSKEATGLGKIVKTSETLNKEQVLGIRDLTRYDPGVAVVEQGNGASGGYS
                     IRGVDKNRVAVSVDGVAQIQAFTVQGSLSGYGGRGGSGAINEIEYENISTVEIDKGAG
                     SSDHGSGALGGAVAFRTKEAADLISDGKSWGIQAKTAYGSKNRQFMKSLGAGFSKDGW
                     EGLLIRTERQGRETRPHGDIADGVAYGINRLDAFRQTYDIQSKDKTGQFLVEGENTLK
                     PVAKLAGYGIYLNRQLNRWVKERIEKNQPLSAEEEAMVREAQARHENLSAQAYTGGGR
                     ILPDPMDYRSGSWLAKLGYRFGGRHYVGGVFEDTKQRYDIRDMTEKQYYGTDEADKFR
                     DKSGVYDGDDFRDGLYFVPNIEEWKGDKNLVKGIGLKYSRTKFIDEHHRRRRMGLLYR
                     YENEAYSDNWADKAVLSFDKQGVATDNNTLKLNCAVYPAVDKSCRASADKPYSYDSSD
                     RFHYREQHNVLNASFEKSLKNKWTKHHLTLGFGYDASKAISRPEQLSHNAARISESTG
                     FDDNNQDKYRLGKPEVVEGSVCGYIETLRSRKCVPRKINGSNIHISLNDRFSIGKYFD
                     FSLGGRYDRQNFTTSEELVRSGRYVDRSWNSGIVFKPNRHFSLSYRASSGFRTPSFQE
                     LFGIDIYHDYPKGWQRPALKSEKAANREIGLQWKGDFGFLEISSFRNRYTDMIAVADQ
                     KTKLPDSTGRLTEIDIRDYYNAQNMSLQGINILGKIDWNGVYGKLPEGLYTTLAYNRI
                     KPKSVSNRPDLSLRSYALDAVQPSRYVLGFGYDQPEGKWGANIMLTYSKGKNPDELAY
                     LAGDQKRYSTKRASSSWSTADVSAYLNLKKRLTLRAAIYNIGNYRYVTWESLRQTAES
                     TANRHGGDSNYGRYAAPGRNFSLALEMKF"
     gene            complement(2930..5083)
                     /locus_tag="WIC03_001420"
     CDS             complement(2930..5083)
                     /locus_tag="WIC03_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ3883903.1"
                     /translation="MCKPNYGGIVLLPLLLASCIGGNFGVQPVVESTPTAYPVTFKSK
                     DVPTSPPAGPSVETTPVNRPAVGAAMRLLRRNIAFHREDGTEIPNSKQAEEELSFKEE
                     DILFLYGSKKDKLQQLKDKIHQRNPNVEIRTSENENKKYGYEFVDAGYVYTTKGKDEI
                     EWTSNHKQFTYRFGYDGFVYYSGEHPSQSLPSAGTVKYSGNWQYMTDAIRHRTGKAGD
                     PSEDLGYIVYYGQNVGATSYAATADDREGKHPAEYTVDFGKKTLTGKLIKNQYVQKKT
                     DEKKPLTIYDITATLDGNRFTGSASVNPDLATNHANKEHLFFHTNADQRLEGGFFGDN
                     GEELAGRFISNDNSVFGVFAGKQKTDASGTKPALPSGKHTKILDSLKISVDEATDDHA
                     RKFAISTMPDFGHPDKLLVEGREIPLVSQEKTIELADGRKMTIRACCDFLTYVKLGRI
                     KTDRPASKPKTEDKGEDEEDTVGNGEEDEDEIGDEENTEDETVEDEVEEDEEEIAEEE
                     ETEEEVDEAEEEEVEETEEESPVEESGGDSGSILPTPEAPKGRDIDLFLKGIRTAEAD
                     IPKTGTAHYTGTWEARIGVPDKKDDQLYGTTFIQKDSYANQGAKAQFTVDFGKKSISG
                     TLTEKNDTHPAVYIEKGVIDGNGFHATARTRDNGIDLSGQGSTNPQRFEANNLLVTGG
                     FYGPQAAELGGNIIDSDRKFGAVFGAKKDDKEATR"
BASE COUNT         1108 a         1381 c         1224 g         1496 t
ORIGIN      
        1 gaaatcttgt ttatattgaa tcaaaaaaac ctgcacctta atcagttggc ggtttagtcc
       61 gacttttggg gtgcagatca agctttcaga cggtatttcc tttaaaactt catttcgagc
      121 gcgagactga agttcctgcc cggtgcggca taccttccat agttgctgtc gccgccgtgc
      181 cggtttgccg tgctttccgc agtctggcgc aaggattccc aagtaacgta gcggtagttg
      241 ccgatattgt agatagccgc cctcaaggtc agccgttttt tcagattcag ataggcggaa
      301 acgtctgccg tcgaccaaga agacgacgct ctttttgtcg aatatcgttt ttgatcgcct
      361 gccagataag caagctcgtc agggtttttc cctttggaat aggtcagcat aatgtttgcg
      421 ccccatttcc cctcgggctg gtcgtatccg aaccccaaaa cataacgcga cggctgtacc
      481 gcatccaaag catagctgcg gagggacaag tccggccggt tggataccga tttcggtttg
      541 atacggttgt acgccaaagt cgtgtacaaa ccttcaggca gtttgccgta cacgccgttc
      601 cagtcgattt ttcctaatat attaatgcct tggagcgaca tattttgggc attgtaataa
      661 tcgcgtatat caatctctgt caatcgtcct gttgaatccg gcaatttggt tttttgatcg
      721 gcaacggcaa tcatatcggt atagcggttg cggaagctgc tgatttccaa aaagccgaaa
      781 tcgcccttcc actgcaaacc gatttcgcgg ttggctgcct tttccgattt cagggcggga
      841 cgctgccagc ctttcggata atcgtgataa atgtctatcc cgaaaagttc ttggaatgag
      901 ggcgttctga agccgctgga ggcgcggtaa gacaaagaaa aatgccggtt cggtttgaac
      961 acgatgccgc tgttccacga acggtcaaca taccgcccgc tgcggacgag ttcttccgac
     1021 gtggtaaagt tttgccggtc gtacctgccg cccaagctga aatcgaaata tttgccgatt
     1081 gaaaaacggt cgttcaaaga aatgtggata ttgctgccgt tgatttttct tggcacgcat
     1141 ttacgggaac gcagggtttc gatgtagccg cagaccgacc cttcgacgac ttcgggctta
     1201 cccaaacggt acttatcttg attgttatca tcgaatcccg tggattccga aatccttgcc
     1261 gcattgtggg aaagctgttc ggggcgggag atcgctttgg aagcatcgta accgaagccc
     1321 aaagtcagat ggtgtttcgt ccatttgttt ttcagcgatt tctcaaacga ggcattcaaa
     1381 acattgtgct gttcgcggta gtggaaacgg tcgctgctgt cgtaggaata cggtttgtcc
     1441 gccgacgcgc ggcaggattt gtccacggca ggatacacgg cgcaattcag cttcagcgtg
     1501 ttgttgtcgg ttgccacgcc ctgtttgtca aacgacaaca ccgccttatc cgcccagttg
     1561 tcagaatacg cttcgttttc ataacgatac agcaaaccca tacggcggcg gcggtgatgt
     1621 tcgtcaataa atttggtgcg ggaatatttc aaacctatgc ccttgaccaa atttttatcg
     1681 cccttccact cttctatatt cggcacaaaa tacaagccgt cgcggaaatc gtcgccgtcg
     1741 tacaccccgc tcttgtctct aaacttgtcc gcctcgtccg taccgtaata ctgtttttcc
     1801 gtcatatcgc ggatatcgta acgctgtttg gtatcctcaa acacgccgcc gacataatgc
     1861 ctgccgccga agcggtagcc cagcttggca agccacgaac cgctgcggta atccatcgga
     1921 tcgggcaata tcctgccgcc gcccgtgtaa gcttgggcgg acaggttttc gtggcgcgcc
     1981 tgcgcctccc gcaccatcgc ttcttcttca gcacttaaag gctgattttt ttcaatacgt
     2041 tcttttaccc agcggttaag ctggcggttc aaatatatcc cgtagcccgc caattttgcc
     2101 acgggcttga gtgtattctc gccctctact aagaattgcc ctgttttatc tttgctttga
     2161 atatcgtatg tctggcggaa cgcgtccaaa cggtttatgc cgtatgccac cccgtccgca
     2221 atatcgccgt gcgggcgcgt ttcccgccct tggcgttcgg tacggattag cagcccttcc
     2281 caaccgtctt tgctgaaacc cgcgccgagc gacttcataa attggcggtt tttactgccg
     2341 taggcggttt ttgcctgtat cccccagctt ttaccgtctg aaatcaggtc tgccgcttct
     2401 ttggtgcgga aagcgaccgc gccgccgagt gcgccgctgc cgtgatcgga cgaaccggca
     2461 cctttgtcga tttccaccgt gctgatgttt tcatattcga tttcgttgat tgcgccgctg
     2521 ccgccgcgtc cgccgtatcc gctcaacgat ccctgcacgg taaacgcctg tatttgggca
     2581 acgccgtcaa ccgaaaccgc cacacggttt ttatccacgc cgcgtatcga gtagccgccg
     2641 ctcgcgccgt tgccctgttc gacaaccgcc acgcccggat cgtagcgcgt caggtcgcgg
     2701 ataccgagta cctgttcttt gttcaacgtt tccgacgttt tgacgatttt gcccaaaccg
     2761 gtcgcctctt tcgatcgccg tcccactttg gcggcacgga cggtaacctc tttcagggat
     2821 tgggtctgcg ccgcatcagg tgtcgccgtt tcagggtttg ccgcatatga cgggaaggcg
     2881 gcggcaacag ccaaggcagt cagggtaagc gggaatgtat gctttctgtt catcgtgttg
     2941 cctccttgtc atctttcttc gcaccgaata ccgcgccgaa tttccggtcg ctgtcgataa
     3001 tattaccgcc caattccgcc gcctgcggtc cataaaagcc gcctgttaca agaagattgt
     3061 tggcttcgaa gcgctgcggg ttggtcgaac cctgcccgga aaggtcaata ccgttatccc
     3121 gagtgcgtgc tgtcgcgtgg aaaccgttgc catcaatcac acctttttca atataaacag
     3181 cggggtgtgt atcatttttt tctgtcaacg ttccggaaat cgatttcttg ccgaaatcaa
     3241 cggtaaattg tgcttttgcc ccttgatttg catagctatc cttttgaatg aacgtagtgc
     3301 catataactg atcgtctttc ttatccggta caccgatacg cgcttcccaa gtgccggtat
     3361 aatgcgccgt tccggttttt ggaatgtcgg cttccgccgt gcggatacct ttcaggaaaa
     3421 ggtcgatgtc cctgccttta ggggcttccg gagtgggcag gatgctgcct gaatcgccac
     3481 cgctttcttc caccggcgat tcttcttcgg tttcttcaac ttcctcctct tcggcttcat
     3541 caacttcttc ttcggtttct tcttcttcag cgatttcttc ttcgtcttcc tcgacttcat
     3601 cttctacggt ttcatcttcc gtgttttctt catcgccgat ttcgtcttcg tcttcttcgc
     3661 cgttaccaac tgtatcctct tcatcctccc ctttatcttc cgtctttggt ttacttgccg
     3721 ggcggtcggt ttttatccgt ccgagtttca cataggtcag aaaatcgcaa caagcacgga
     3781 tggtcatttt cctgccgtcg gcaagctcga tggttttctc ttggctaacc aaaggaattt
     3841 cacgcccttc gacaagaagt ttgtcgggat gaccaaaatc gggcatagtg gaaatggcaa
     3901 acttacgggc atggtcatca gtcgcctcgt caacggaaat tttcagagaa tccaagattt
     3961 tggtgtgttt tccagacggc agggcaggtt ttgtgcctga tgcgtctgtt ttttgtttgc
     4021 ctgcgaatac gccgaatacg ctgttgtcgt tgctgataaa ccgtccggca agctcttctc
     4081 cgttatctcc gaaaaaaccg ccctcaagcc gctgatcggc attggtatgg aaaaacaaat
     4141 gctccttatt ggcatggttt gtcgctaaat caggattgac actagcactg ccggtaaagc
     4201 ggttgccgtc caatgttgca gtaatgtcgt aaatggtcag cggtttcttt tcatcggttt
     4261 tcttttgcac atactgattt ttaattaatt tacccgtcaa agttttctta ccgaaatcta
     4321 ccgtatattc ggcaggatgt tttccctctc ggtcgtcggc agtcgcagca taagaagttg
     4381 ctccgacatt ttgaccgtaa taaacgatat aacccaaatc ttcgctagga tctcctgctt
     4441 ttcctgttcg atgacgtatg gcatcggtca tatattgcca gttgccggaa tatttcaccg
     4501 ttcccgcgct cggtaaagat tgcgaaggat gttctccgga ataatataca aaaccgtcat
     4561 aaccaaaccg gtaggtaaac tgcttgtgat ttgaagtcca ctcaatttca tcttttccct
     4621 ttgtagtata tacataacct gcatctacaa attcataacc atatttttta ttttcatttt
     4681 ctgatgtcct aatttctaca ttaggattgc gttgatgaat tttatcctta agctgctgaa
     4741 gtttatcttt tttggaaccg tataaaaaca ggatatcttc ctctttaaac gacagctctt
     4801 cttctgcttg tttgctattt ggaatttccg tgccatcttc acgatgaaaa gcaatattcc
     4861 gcctcaacag ccgcattgcc gcaccgacgg cgggccggtt gaccggcgtg gtttctaccg
     4921 aaggcccggc agggggcgaa gtgggaacgt ccttagactt gaaagtgacg gggtacgcgg
     4981 tcggcgttga ttcgacaaca ggctgcacac cgaaattgcc gccgatgcaa gatgccaaaa
     5041 gtaagggcaa caagacaatg ccgccataat tcggtttaca catccctact tttcctccta
     5101 tttgattaat aataattatc attatattaa tatgtacaga taatatcaac ccgtttttat
     5161 agtgaattaa caaaaatccg gacaaggcga cgagccgcag acagtacag
//
LOCUS       DAWHSK010000133         5200 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.133, whole genome shotgun sequence.
ACCESSION   DAWHSK010000133 DAWHSK010000000
VERSION     DAWHSK010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5200
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.133"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(23..439)
                     /gene="dksA"
                     /locus_tag="WIC03_001421"
     CDS             complement(23..439)
                     /gene="dksA"
                     /locus_tag="WIC03_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HEZ3883904.1"
                     /translation="MAKLTEQDILNWSGPEDDYMNDDHLAFFRELLVKMQDELIENAS
                     ATTGHLQEHESAPDPADRATQEEEYALELRTRDRERKLLSKIQATIRNIDEGDYGFCA
                     DTGEPIGLKRLLARPTATLSVESQERRERMKKQFAD"
     gene            complement(586..1377)
                     /locus_tag="WIC03_001422"
     CDS             complement(586..1377)
                     /locus_tag="WIC03_001422"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEZ3883905.1"
                     /translation="MNVYFLGGGNMAAAIAGGLVKQGGYRIYIANRGAEKRERLQKEL
                     GVETSATLPELHSDDVLILAVKPQDMEAACKNIRTNGALVLSVAAGLSVDTLSRYLGG
                     NRRIVRVMPNTPGKIGLGVSGMYAEAEVSETDRRIADRIMKSVGLTVWLEDEAQMHSI
                     TGISGSGPAYVFYLLDALQNAAIRQGFDMAEARALSLATFKGAVTLAEQTGEDFEKLQ
                     KNVTSKGGTTHEAVEAFRRHRVAEAISEGVCACVRRSQEMERQYQ"
     gene            complement(1426..1833)
                     /locus_tag="WIC03_001423"
     CDS             complement(1426..1833)
                     /locus_tag="WIC03_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEZ3883906.1"
                     /translation="MEIRAIKYTAMAALLAFTVAGCRLAGWYECSSLSGWCKPRKPAA
                     IDFWDIGGESPPSLEDYEIPLSDGNRSVRANEYESAQQSYFYRKIRKFEACGLDWRTR
                     DGKPLIETFKQEGFDCLKKQGLRRNGLSERVRW"
     gene            complement(1833..2519)
                     /locus_tag="WIC03_001424"
     CDS             complement(1833..2519)
                     /locus_tag="WIC03_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEZ3883907.1"
                     /translation="MTVLQERYCEVSDRIGKLVLQAGRKPHSVSLIAVGKTFPSDGIR
                     EVYAAGQRDFGENYIQEWYGKTEELADLTDIVWHVIGDVQSNKTKFVAERAHWVHTVC
                     RLKTAVRLSEQRPSSMPPLQVCIEVNIAGEAVKHGVAPEEAVALAVEVAKLPNIVVRG
                     LMCVAKANSSETELKVQFQTMRKLLADLNTAGVKADVLSMGMSDDMPAAIECGATHVR
                     IGSAIFGKRG"
     gene            2639..3682
                     /locus_tag="WIC03_001425"
     CDS             2639..3682
                     /locus_tag="WIC03_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEZ3883908.1"
                     /translation="MQITDLLAFGAKNKASDLHLSSGISPMIRVHGDMRRINLPEMSA
                     EEVGNMVTSVMNDHQRKIYQQNLEVDFSFELPNVARFRVNAFNIGRGPAAVFRTIPST
                     VLSLEELKAPSIFQKIAESPRGMVLVTGPTGSGKSTTLAAMINYINETQPAHILTIED
                     PIEFVHQSKKSLINQRELHQHTLSFANALRSALREDPDVILVGEMRDPETIGLALTAA
                     ETGHLVFGTLHTTGAAKTVDRIVDVFPAGEKEMVRSMLSESLTAVISQNLLKTHDGNG
                     RVASHEILIANPAVRNLIRENKITQINSVLQTGQASGMQTMDQSLQSLVRQGLIAPEV
                     ARRRAQNSESMSF"
     gene            3845..5071
                     /locus_tag="WIC03_001426"
     CDS             3845..5071
                     /locus_tag="WIC03_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEZ3883909.1"
                     /translation="MNTDNLHDILDEMVQVYSQKKQSRSETPAEIGAHFHPLLDRLCE
                     TAEAQNASDILISKGFPPSLKINSALTPQPQKALTGEETAAIAASTMNAEQSEIFRRD
                     GEINYSVQSRSGTRYRANAYHSQGSAGLVLRRINHVIPQMQELGLPEKLKDLAVAPRG
                     LLIIVGPTGSGKSTTMATMLEHRNKTLPSHIVTIEDPIEFIYKPRRCIFTQREIGVDT
                     INWQTAVQNAMRQSPDVVCIGEVRSRESMEYAMQLAQTGHLCIFTLHANTAPQSLERI
                     LNFYPKEQHNQILIDIALNLTGIICQRLALKQDKTGRTAVVDLLINTPAIQDFILKGD
                     LMNISKIMETAKTDGMQTMDQNLFELYRHGIISYEEALRQSVSANNLRLHIQLHKEGK
                     TPELLYDRVNGLNLIS"
BASE COUNT         1299 a         1601 c         1174 g         1126 t
ORIGIN      
        1 gacggcattt tgtttgccgc catcagtcgg caaactgttt tttcatcctc tctcggcgtt
       61 cttgggactc aacagataaa gtggctgtcg ggcgtgccag cagccgcttc agaccgatag
      121 gctctcccgt atcggcacag aatccataat ccccttcatc aatattgcgg atggtcgcct
      181 gtattttact gagaagtttt cgttcccgat cgcgggtacg gagttccaat gcgtactctt
      241 cttcctgtgt ggcacggtcg gcaggatcgg gggctgattc gtgttcttgg agatgccctg
      301 tcgtagcgga agcattttcg atgagttcgt cttgcatttt taccagcaat tcgcggaaaa
      361 aagccaaatg gtcgtcattc atataatcgt cttccggccc gctccaattc aaaatatctt
      421 gttctgtcag ctttgccata atgtatatcc tctcggttca ccgaaaaaaa caaagcacca
      481 caaagtggct ttattccttt gatttttata aggcttattg cctaaggcgt ttttgaaaac
      541 gcgcatcata acacgttttg gattggtttt tttattttct ttacattatt gatattgccg
      601 ttccatttcc tgcgaacggc gcacacaggc acaaacgccc tcgcttatgg cttcggcgac
      661 acgatgccgc ctgaaagctt ccacggcttc gtgggttgtc ccgccttttg acgttacatt
      721 tttttgaagc ttctcgaaat cttcacccgt ctgctcggca agggtaaccg ctcctttaaa
      781 cgttgccaga ctgagcgcgc gtgcttctgc catatcaaac ccttgtcgga tggcggcatt
      841 ttgcaatgcg tccagcagat aaaacacata agccggtccg ctgccgctga taccggtaat
      901 gctgtgcatt tgcgcttcat cttccaacca aacggtcaaa ccgactgatt tcatgattcg
      961 atcggcaatc ctgcggtctg tttccgatac ttccgcttcg gcatacatac cggatacgcc
     1021 cagtcctatt tttccgggtg tattcggcat aacccggaca atgcggcggt ttcccccgag
     1081 gtaacggctg agcgtatcaa ccgacaatcc agctgcaaca gaaagcacca atgcgccgtt
     1141 ggtgcggata tttttgcacg cggcttccat atcctgcggt ttgacggcaa ggattaaaac
     1201 atcgtcggaa tgaagctccg gcagggttgc cgaagtttcg acccccaact ctttttgcaa
     1261 acgttcgcgt ttttccgcac cccgattggc tatatagatg cggtaaccgc cttgtttgac
     1321 caatccgcct gcgatggcag ccgccatatt gccgccgccg agaaaataaa cattcattat
     1381 ttctacccta ttcaaaatta cctgactaaa ttcattccca attttttacc atcggacgcg
     1441 ctcggacaga ccgttgcgcc gcaacccctg ctttttcaaa caatcaaaac cttcctgttt
     1501 gaacgtctca atcaaaggtt tgccgtcacg cgtacgccaa tccaacccgc aggcttcaaa
     1561 cttccttatt ttcctgtaaa agtaagattg ttgtgcggat tcatattcat ttgccctgac
     1621 ggaacgattg ccgtctgaaa gcggtatctc gtagtcctct aaagacggcg gactctcgcc
     1681 gccaatatcc caaaaatcga tggcggcagg ttttctcggc ttacaccagc cggacaggga
     1741 cgaacactca taccaacctg ccaaccggca gcctgcaacc gtaaatgcaa gcaacgcagc
     1801 cattgccgta tattttattg cccgaatttc catcagcccc ttttcccgaa aatcgcgctg
     1861 ccgatacgga cgtgtgtcgc accgcactca atggcggcag gcatatcgtc cgacatcccc
     1921 atagacagca cgtcagcctt aacgccagcc gtattgaggt cggcaagcag tttccgcatc
     1981 gtttgaaatt gcaccttcaa ctccgtttca ctgctgttgg ctttggcaac acacatcagt
     2041 ccgcgtacga cgatattcgg cagcttcgcc acttccacag caagcgcgac tgcttcttcg
     2101 ggcgcgacac cgtgcttcac cgcctcgccc gcaatattca cctcgataca cacctgcaag
     2161 ggcggcattg aggaaggacg ctgttcgctc aaccggacag cggttttcag acggcatacg
     2221 gtatgcaccc aatgcgcgcg ttcggcgaca aacttggttt tgttggactg cacatcgccg
     2281 atgacgtgcc acacgatgtc ggtcaaatcc gccaactctt ccgttttgcc gtaccactcc
     2341 tgaatatagt tctcgccgaa atcacgctgt ccggcggcgt aaacttcgcg gatgccgtct
     2401 gaagggaaag tcttaccgac ggcaatcagg ctgacggaat gcggcttcct tcccgcctgc
     2461 agaaccaatt ttccgatacg gtcggacacc tcacaataac gttcttgcaa caccgtcata
     2521 gattatcccc taattaaaaa tgattaaaca gttgaaaccc ctccagtcag ggcggtacaa
     2581 tcaaggttgt tagaaccatt ccaaccaatc gaaacattat actaaacaga gccgcattat
     2641 gcagattacc gacttactcg ccttcggcgc taaaaacaaa gcatccgacc ttcacctgag
     2701 ttcgggcata tcccctatga ttcgggttca cggcgatatg cggcgcatca accttcccga
     2761 aatgagcgcg gaagaggtcg gtaatatggt aacttcggtg atgaacgacc accagcggaa
     2821 aatctaccag caaaacttgg aagtcgactt ctcgttcgaa ctgcccaacg tcgcccgatt
     2881 ccgcgtcaac gccttcaaca tcggccgcgg tcccgccgcc gtattccgca ccattcccag
     2941 caccgtctta tcgctggaag aattgaaagc cccgagcatt ttccaaaaaa tcgcagaatc
     3001 gccgcgcggc atggttttgg ttaccggccc taccggttcg ggcaaatcga ccacgcttgc
     3061 cgcaatgatc aactacatca acgaaaccca gccggcacac atcctgacca tcgaagaccc
     3121 gatcgaattc gtccaccaaa gcaaaaaatc cctgattaac cagcgcgagc tgcaccagca
     3181 caccctcagc ttcgccaacg cgctgcgttc cgcattgcgc gaagaccccg acgttatcct
     3241 tgtcggcgag atgcgcgacc cagaaaccat cggcttggcg ctgaccgccg ccgaaaccgg
     3301 acacttggtt ttcggcacgc tgcacacgac cggcgcagca aaaaccgtcg accgtatcgt
     3361 ggacgtattc ccggcgggag aaaaagaaat ggtgcgctct atgctgtccg aatcgctgac
     3421 cgccgtcatc tcccaaaacc tgctgaaaac gcacgacggc aacggccgtg tcgcctcgca
     3481 cgaaatcctg attgccaacc ccgccgtccg caacctcatc cgcgaaaaca aaatcacgca
     3541 gattaactcc gtcctacaaa ccgggcaggc gagcggtatg cagacaatgg accaatcgct
     3601 gcaatcgctg gtgcgccaag ggctgatcgc accggaagtc gcacgcagac gcgcgcaaaa
     3661 cagcgaaagt atgagtttct gacacacaac cgccttccgg ccataccggc gggaaaacaa
     3721 ggcgcaaaca cgcggggcgg gacgcagcat cccgcccggc tacctttccg aacaggaagc
     3781 gtccgccttc ctgttgaaac ctgccgccgc aaactgcaag gcttaaaccg aaaagaagct
     3841 aacgatgaat accgataacc tgcacgacat cttggacgaa atggttcaag tgtattctca
     3901 aaaaaaacaa agccgatccg aaaccccggc cgaaatcggc gcacacttcc acccgctgct
     3961 cgaccgcctg tgcgaaaccg cagaagcaca aaacgcgtcc gacatcctta tcagcaaagg
     4021 attcccgccc tcgttgaaaa tcaacagcgc attaaccccg cagccgcaaa aggcgctgac
     4081 gggcgaggaa accgccgcca tcgccgcatc gacgatgaac gccgaacaat cggaaatatt
     4141 ccggcgcgac ggcgaaatca actactccgt ccagtcgcgc agcggcacgc gctaccgcgc
     4201 caacgcctac cacagccaag gcagcgcagg tttggttttg cggcgcatca accacgtcat
     4261 cccgcaaatg caggaattgg gtctgcccga aaaactcaaa gacctcgccg tcgcaccgcg
     4321 cgggctgctg attatcgtcg ggcctaccgg ttcgggcaaa tccaccacga tggcgactat
     4381 gctcgaacac cgcaacaaaa ccctgcccag ccatatcgtt accatcgaag acccgattga
     4441 gtttatctac aaaccgcgcc gctgcatctt tacccagcgc gaaatcggcg tcgacaccat
     4501 aaactggcag acggcggtac aaaacgctat gcgccagtcc cccgacgtgg tctgcatcgg
     4561 cgaagtccgc agcagggaaa gtatggaata cgcgatgcag ctcgcccaaa ccggccacct
     4621 gtgcattttt acgctccacg ccaacaccgc gccgcagtcg ctcgaacgca tactcaactt
     4681 ctaccccaaa gaacagcaca accaaatact gatcgacatc gccctcaacc tgaccggcat
     4741 catctgccaa cgcctcgccc tcaaacaaga caaaacgggc aggacggcgg ttgtcgactt
     4801 gctcatcaac acgcccgcca tccaagactt catcctgaag ggcgacctga tgaacatcag
     4861 taaaatcatg gaaaccgcca aaaccgacgg aatgcagacg atggatcaaa accttttcga
     4921 actgtaccgt cacggcatca tcagttacga agaagccctg cgccagtccg tttccgccaa
     4981 caacctgcga ttgcacatcc aactgcacaa agaaggcaaa acgcccgaac tcctttacga
     5041 cagggtcaac ggtctcaacc tcatttcctg atccgcaaaa cccaatgccg tctgaaaacc
     5101 gcatccccgt tttcagacgg catgatttta tccgccccat tcatgtgcta cactttatag
     5161 tggattaaat ttatagtgga ttaacaaaaa tcaggacaag
//
LOCUS       DAWHSK010000134         5164 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.134, whole genome shotgun sequence.
ACCESSION   DAWHSK010000134 DAWHSK010000000
VERSION     DAWHSK010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5164
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.134"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            629..1045
                     /locus_tag="WIC03_001427"
                     /pseudo
     CDS             629..1045
                     /locus_tag="WIC03_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360042.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1221..1457
                     /locus_tag="WIC03_001428"
                     /pseudo
     CDS             1221..1457
                     /locus_tag="WIC03_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086896509.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1510..1698)
                     /locus_tag="WIC03_001429"
     CDS             complement(1510..1698)
                     /locus_tag="WIC03_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HEZ3883910.1"
                     /translation="MTVKYTIFFSTSHHSNQTKATHRHTHVGSTAGQTTIRSGGDTTL
                     KGAQLIGIGIQADPQLQP"
     gene            complement(1843..2793)
                     /locus_tag="WIC03_001430"
     CDS             complement(1843..2793)
                     /locus_tag="WIC03_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEZ3883911.1"
                     /translation="MYLGIDVSKLTIDCCLIVDGQNYQKKFQNNKGGFEQLINWLQSH
                     KVNDKLHCVCEATGTYYEALAEYLYSRYTITVENPRKIKGYAIAELQRSKTDTQDAKL
                     IAQYCQDRKHKLKAWKPPTKEQKQLQEIARYLDYLKQQRATEKAKQHEAPDYIKSHIQ
                     TTISNLTAQIQIVKKQLLQFYKDNPSYNNLRKRLKTITGIGEQATAVLLSTYKRHEFK
                     NARQFTAYLGLDPRKFQSGTSVNGKSRISKIGSSEIRKSLYMPALVAYRCNAFPEFVG
                     RLKNKGKHIKLILIAIMRKLAVIAFTILQNGQDFQVERYK"
     gene            complement(3419..4504)
                     /locus_tag="WIC03_001431"
     CDS             complement(3419..4504)
                     /locus_tag="WIC03_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zonular occludens toxin domain-containing
                     protein"
                     /protein_id="HEZ3883912.1"
                     /translation="MIYLFTGNMGTGKTSRVVSMILNNEDGLFKMKLEDGTEVDRPLY
                     FCHIDGLDKRQFKAHELTEEQIMSAPLRDVIPEGAVLIVDEAHYTYPVRAAGRPVPPY
                     IQELTELRHHGHTVILMTQHPSQLDIFVRNLVSKHVHLERKAIGMKQYYWYKCVTSLD
                     NPAGVSGVEVASWKPPKEAFKYYKSASQHQKFKKKVPWAVWALIAIVGFVGWKSYGIF
                     KVYSKATDSRIEQEAQKESVVQTMTEQPASSEEMPLKNSDNLKPEDFVPTLPEKPESK
                     PIYNTVRQVKTFEQIAGCIDGGKSDCTCYSNQGTPLKEITKIMCKEYVKNGLPFNPYK
                     DEQQRTEQVEQSAKADKPQVLVMGGKP"
     gene            complement(4514..4804)
                     /locus_tag="WIC03_001432"
     CDS             complement(4514..4804)
                     /locus_tag="WIC03_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2523 domain-containing protein"
                     /protein_id="HEZ3883913.1"
                     /translation="MPLLAGLIPLLGILLKMLIVRIILATGLTFVTYAGYLIALEKFK
                     DYTSNAINSMPSDILNLLLISGFGQGLGYLFGAFSFFIGMHAFKKLTFVFPG"
     gene            complement(4805..>5164)
                     /locus_tag="WIC03_001433"
     CDS             complement(4805..>5164)
                     /locus_tag="WIC03_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T cell/B cell stimulating protein TspB"
                     /protein_id="HEZ3883914.1"
                     /translation="VPDRPNGRHRKERKEGEDGGLLCDYFPEILACQEMGKPSDGMFH
                     DISIPQVIDDKTWSSHNFLPSNGVCPQPKTFHVFGRQYQASYEPLCVFAEKIRFAVLL
                     AFIIMSAFVVFGSLKGK"
BASE COUNT         1305 a         1122 c         1124 g         1613 t
ORIGIN      
        1 tatcggcggc caaagtaccg ctgccattgc cgatctcgcc ggggaaatga gaaggctcgc
       61 taaaaaatca aaaatgttac tgaaaggact ttggccattt ttatcctcta caatatctgc
      121 atttaaaata aatcagtcat tgtttaaaca ttactgtaaa actgtacaac tgctaaactg
      181 taaacacaga aagaagaatt actaacagca caaaacaaca gatatccaaa caccattaag
      241 tgcattattc tgcattttcg ttgcctgatg tattgatgct atttgtgctg ttcttatata
      301 tgttgatgct ggttacgtcg cgttttttcg gaaaattcaa ccggcaggga gccgatacgc
      361 tgtttcatct ctttgcttag ggagagtagg gggcggatac gaccttagtt ttggtatccg
      421 taatatcatc atttttttcg tcaagggaat atacacggag gatgattcaa aaccggcagg
      481 caaaaaaaga ccgcttctga actgcacccc aaaagttgga caccttaccg actttagggg
      541 gtgcagtttt ttatggcaaa atattcagat gaattccgac ttgccgtcgt tcaatactat
      601 ttggcaggga acagcagacc atctttctat ttccgattca ttggtacgca gatgggtgac
      661 aaaatacaga ttacacggag agagtggcat caaacgtaga aagcatacga caaaatattc
      721 ggtcgaatac aaacttgagg caatccgcct ggtgacgggg tagggaatgt cccaaaaagc
      781 tgccgcagac caactgaatt tgcccgactg ctccatcttg ctgcaatggt tgcgcctcta
      841 ccatttgaat ggtattaacg gtttaaagcc caaaccctaa acccaaagga agaaagcccg
      901 tgaaaaaaca gcatccgccg gaaacgaaaa agccgactat ctgaaaacca aggaagaact
      961 gcttgcggaa ttggcttgcc ttaaagcgga aatggctgcc ctaaaaaagc tcgatgcctt
     1021 aatctatggg aaagaagtgc ggtagaaaga acgcaactcg tcgcagggtt aaggcaatgc
     1081 catccgttga actgctgttg gtgattgtcg gactgccacg cggcaccttc tattaccaat
     1141 tggttgtcca atcggcagaa gacaaatatg ccgatttgaa acggcatatc catgatattt
     1201 atcaaaagca gttgaaagac aacggtctgg ttcagagtat gtcccgcaag ggaaactgct
     1261 tggacaatgc ggcaatggaa agtttcttcg gaacgttgaa atcggaatgt ttccatacgt
     1321 gcaaatatga ttccgttacc gaattggaag ctgtactgca cgaatatatc cgttactaca
     1381 acaacgatag aatcaagttg aaattaaaag gactgagccc tgttcagtac agaattcagt
     1441 ccctgaaagc cgcttgatta aactgtccaa cttttggggg tcagttaata tcggttccac
     1501 caatagtcgt cagggttgta gttgggggtc tgcctgtatg cctatgccga taagctgagc
     1561 acctttgagg gtggtatccc cgccgcttcg gatggtggtt tgtccggccg tactgccgac
     1621 atgggtgtgg cggtgggttg cttttgtctg attgctgtgg tggcttgttg aaaaaaagat
     1681 agtgtatttc acggtcaaat aactttacat ttcttgaagt tgcaagaatg cgcaccccga
     1741 ctttttcggg atgtttgttt cgttttgaat ggatttgatg ttttaattta tactttattt
     1801 tcaataaatt gaaaatcacc ggcgaaagcc cagtttaatt ttttatttat atctttccac
     1861 ttggaaatct tggccgtttt gcaaaatcgt aaacgctatt accgccagtt tccgcatgat
     1921 ggcaattaat atcaatttta tatgcttccc tttatttttc agacgcccta caaattcagg
     1981 gaaggcatta caacgatatg caacaagtgc aggcatataa aggcttttcc ttatttccga
     2041 acttcctatt tttgatattc tgctttttcc gttcacgctt gttcctgatt gaaattttct
     2101 agggtctagg cctagataag ccgtgaactg tcttgcattt ttaaattcat gtcttttata
     2161 ggttgatagc aatactgctg tcgcttgctc gcctatgcct gttattgttt tcagcctttt
     2221 gcgtagattg ttataacttg gattgtcttt gtagaactgg agtaattgct ttttgactat
     2281 ctgtatttgt gctgtcaggt ttgaaatagt tgtttgaata tgggatttga tatagtcggg
     2341 tgcttcgtgt tgtttagctt tttctgttgc gcgttgctgt ttcagatagt ctaaatatcg
     2401 ggcgatttcc tgtaattgct tctgttcttt tgtcggcggt ttccatgctt ttaatttgtg
     2461 ctttcggtct tggcaatatt gggctatcaa ctttgcgtct tgtgtatctg tttttgatcg
     2521 ttgtagttct gctatcgcat atccttttat ctttcgtgga ttctctacgg taattgtata
     2581 tcttgaataa agatattcgg ctaatgcttc gtaatatgtg cctgttgctt cgcacacgca
     2641 atggagctta tcgtttactt tatgactttg tagccaattt attaattgtt caaatcctcc
     2701 tttgttgttc tgaaacttct tttgataatt ttgaccgtct acaatcaaac agcaatctat
     2761 tgtgagcttt gaaacgtcta ttcctaagta catggtttaa ccttattaat tcgggctttt
     2821 tgccctagat agtgttcaaa cttaagatgt acgaaagccc acgcttctat ctttcttaca
     2881 agctgtacgc tttggccgta ctttcgaagt cgtgggcttt acttggtgtt tcgtcaaacg
     2941 ccaagccctc aatgggctga tttactcatt cagggcttga agcttatcgc ctttgccgta
     3001 tctgatttta ttggggtcgc aggttttggt aaaggtttct gttgcgaccc gaatgtctga
     3061 ttttttttgg gcgtatctca gtctggaatc actccgttcg ggggtttccg gtattgaaaa
     3121 acagttcata aaaaaggaaa agggggtatt cataaagatt gggtaaaaag cgcgcccaat
     3181 ctttacaaag cttccccctt ttcctttttt ctgccctatt ttcctgcacc tacaaccccc
     3241 gaacgaagcg attccagact gagacacttt aaaaaaaaca gccattctag caattaaccc
     3301 ccttcactca gctcaagcca tcctgagggg tagggattga actttctgct ttacgactcc
     3361 gccgccaatt ccttcaaacg gttttccgcg ctcttcagtt gtcgtacatg agattttgct
     3421 acggctttcc gcccattacg agaacttgcg gcttgtccgc tttcgcggac tgttccacct
     3481 gttccgtcct ttgctgttcg tccttataag gattgaaagg caacccgttt ttcacatatt
     3541 ctttacacat tatctttgtt atttctttca agggtgttcc ttgatttgaa tagcatgtgc
     3601 aatctgattt tccgccgtct atacatccgg cgatttgctc aaaggttttt acttgtcgga
     3661 ctgtgttata aataggcttg ctttcgggct tttcgggtaa agtcggcaca aagtcttcag
     3721 gtttcaaatt gtctgaattt tttaaaggca tttcctctga tgatgcaggc tgctccgtca
     3781 tcgtctgcac aacgctttct ttttgcgctt cctgctcaat ccggctgtct gtggctttgc
     3841 tgtaaacttt aaaaatgccg taacttttcc agcctacaaa ccctacaatc gcaatcaacg
     3901 cccaaaccgc ccaaggtact tttttcttga acttttggtg ctggcttgct gatttatagt
     3961 atttaaaggc ttctttcggc ggtttccaac ttgcgacttc tacgccgctt acgcctgcgg
     4021 gattgtccaa cgaggttacg catttatacc aataatactg tttcattccg attgccttgc
     4081 gttcaaggtg tacatgcttt gaaacaaggt tgcggacgaa tatatcaagt tggctcgggt
     4141 gctgcgtcat caaaataacg gtatgcccgt gatggcggag ttctgtcagt tcctgaatat
     4201 aaggcggaac gggacggcct gccgcgcgta ccggataagt gtagtgcgct tcgtcaacaa
     4261 tcagcactgc gccttccggt atgacatcac gaagcggggc ggacatgatt tgctcttccg
     4321 tcagttcgtg ggctttaaac tgccgtttat ccaatccgtc gatatggcag aaataaagcg
     4381 gtctgtctac ctctgtgccg tcttccaatt tcattttgaa caatccgtct tcgttgttca
     4441 aaatcataga gacgacgcgg gaggttttgc ctgtccccat gtttcctgta aacagataaa
     4501 tcatgcttct acctcatcct ggaaagacaa acgtcagttt tttgaatgcg tgcataccaa
     4561 tgaagaacga gaatgcgccg aacaggtagc ccaacccctg accgaatccc gaaattaaaa
     4621 gaaggttcag tatgtcggaa ggcatggaat tgatcgcatt tgacgtgtag tctttgaact
     4681 tttccagcgc gatgagatac ccggcatagg ttacaaatgt cagacctgtt gcaaggatta
     4741 ttctgacaat cagcattttc agaagtatgc ctaaaagtgg aatcagaccg gcaagtaatg
     4801 gcatttattt ccccttcaac gaaccgaaaa cgacaaaagc cgacataatg ataaaggcga
     4861 gcagtacggc aaaacggatt ttttcggcaa acacgcataa cggctcatag cttgcctgat
     4921 attgcctacc gaaaacatga aaggttttcg gctgcggaca tacgccgtta gacggtaaaa
     4981 agttatgtga agaccatgtt ttatcgtcta taacctgcgg tatgcttata tcgtgaaaca
     5041 tgccgtctga aggtttgccc atctcctgac aggctaggat ttccggaaaa taatcgcaca
     5101 aaagcccgcc gtcttcgcct tctttccttt ctttgcgatg cctaccgttt gggcggtccg
     5161 gaac
//
LOCUS       DAWHSK010000135         5159 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.135, whole genome shotgun sequence.
ACCESSION   DAWHSK010000135 DAWHSK010000000
VERSION     DAWHSK010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5159
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.135"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            296..1729
                     /locus_tag="WIC03_001434"
     CDS             296..1729
                     /locus_tag="WIC03_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEZ3883915.1"
                     /translation="MNEYYQLIKHPDISLSPISDGIGVGNPATGEILAYVRNTGSDKL
                     KNLIQKAAAAQKLWAAKTALERADILWRWYFLVKENKEALARLMTMEQGKSLTESRGE
                     IDYAASFVRWFAEEARRIDGDVLTSVKASQKLVVLKQPVGVTAAITPWNFPSAMIARK
                     AAPALAVGCAMIIKPASLTPLSAYALASLAYEAGIPQDFLPVVSGRASEIGREFATNP
                     IIRKISFTGSTEVGAKIFADSAADIKKLSLELGGNAPFIVFDDADLDKAVEGALASKF
                     RNSGQTCVCTNRVYAQSGIYDEFCRKLSEKVAELKLGNGLEDGVNQGPLIEEKAVEKV
                     EQHIADALAKGAVCLTGGKRSALGGTFFEPTVLSGVTAQMAVAREETFGPLCPVFRFE
                     TEAEVIEAANNTEYGLAAYLFTADTARQWRVGEALEYGMVGINTGLISNEAAPFGGVK
                     RSGLGREGSKYGADEYLELKYLCMDVG"
     gene            1838..2626
                     /locus_tag="WIC03_001435"
     CDS             1838..2626
                     /locus_tag="WIC03_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimb protein"
                     /protein_id="HEZ3883916.1"
                     /translation="MMENGKTVPRWRFALKSAGWHLLISLSVAGLAALLVFKVWYPYP
                     YAELTGGLSLYQLVVAVDIVCGPLLTLILASPKKKTKARMVDFSMVGIIQLAALVYGL
                     HSVSLARPVIEAFEKDRINVVTAAEVAVEDLHKAPEGLQSLSWFGIRRIALKEPEDAD
                     EKNKTLDLSLKGIEPSMRPDRWLPYSDKEAEKIRKHLKPLKVLADARKTTVADILKQA
                     GLAEGEELYYLPFTSSRQKEWIVITDKEGNTKGYAPIDGFIITP"
     gene            2994..4550
                     /locus_tag="WIC03_001436"
     CDS             2994..4550
                     /locus_tag="WIC03_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEZ3883917.1"
                     /translation="MDFSWLAEPHTWIGFATLLVLEVVLGIDNLVFVAILANKVQPAR
                     RDRARIIGLGLAVVIRIIMLAFMAHIITLTEPLFQIGGLAVSGKDMIMLAGGIFLLYK
                     ATTELHERLEGHNRFTVADNQKKHAPFWGVVAQILILDAVFSIDSVITAVAMVDHIVV
                     AMGAVVVAMAVMISASKLLTEFVDRHPTVVMLCLGFLLMIGFSLIAEAFHFHIPKGYL
                     YAAIGFSILIELFNQISQRNSRKNDYIGSSWRKRTAENVLGMMGIRESVLADAGGESG
                     DDAHFEENEKSMIRSVLTLAERPIMGVMIPRRDIERLDISQSREEQYAQLQNTPYSRL
                     LVVGKAGVDEPLGYINKKDLLSQLLETGGLDIQTALRQPLVLPDGTTALGALELFRQS
                     SADYALVVDEFGAVLGMVTMKDLLEAIAGDFPEEFEREEEPAVQGNPDESLTVEGALE
                     YVELASQLNLPQQEEDADFHTVAGLIMEELQTIPDVGDFADFHGWRFEVVEKEGQRIE
                     RVKITKLPEE"
     gene            4567..>5159
                     /gene="surE"
                     /locus_tag="WIC03_001437"
     CDS             4567..>5159
                     /gene="surE"
                     /locus_tag="WIC03_001437"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEZ3883918.1"
                     /translation="MNVLISNDDGYLSEGIAVLARVTAEFANVRVVAPERDRSGVSNS
                     LTLERPLQLKQAQNGFYYVNGTPTDCIHIGQSVFSDFQADFVFSGINRGANMGDDTLY
                     SGTVAAATEAYLMGIPAVAFSLNDASGRYWATAEQALWTLLAHFFKTPPQSPILWNIN
                     IPAVAPEDVRGIKIARLGRRHHGQNVIPARNPRGEQI"
BASE COUNT         1210 a         1302 c         1460 g         1187 t
ORIGIN      
        1 gtttggattt ttgccatact ctaccacacg ttgcaactgc aatctttgtt ccttaatata
       61 ttattaaagg gctgcattac ttttctttct aaattaccat tgtataaaaa gtatttttct
      121 taaacgacag ggattgcgcc cgcgccatat tcagacaccg cccggatgtt gcgctgcccg
      181 atcggatgct tcagacggca tcggaagggt ttgcagtttt ggaatatgaa gatgataatg
      241 tccgcgagat tgacggcatt tgaaagcggt tattccgcat accggaggac acgaaatgaa
      301 cgaatattac caattaatca agcatcccga tatttccctt tccccgattt cagacggcat
      361 cggggtcggc aatccggcaa cgggcgaaat tttggcgtat gtccgcaata cgggttcgga
      421 caaactgaaa aacctgattc aaaaggcggc ggcagcgcaa aaattatggg cggcgaaaac
      481 tgctttggaa cgcgccgaca tattgtggcg ttggtatttt ttagttaaag aaaacaaaga
      541 agcactggcg cgcctgatga cgatggagca gggcaaaagc ctgaccgagt cgcgcggcga
      601 aattgattat gcggcttcgt ttgtgcgctg gtttgccgaa gaggcgcggc ggattgacgg
      661 cgatgtgtta acgagtgtaa aagcgtcgca aaaactggtt gtgttgaaac agcctgtcgg
      721 cgttaccgcc gcgattacgc cgtggaactt cccatctgca atgattgcgc gcaaggctgc
      781 gcctgctttg gcggtgggtt gcgcgatgat catcaaaccc gcatcgctca cgcctttgag
      841 tgcgtatgcc ttggcttcgt tggcttacga agcgggcata ccgcaggatt ttttgcccgt
      901 cgtcagcggc cgcgcatcgg aaatcggccg tgaatttgcc acgaacccga ttatccgcaa
      961 aatcagcttc accggttcga ccgaagtcgg cgcgaaaatt tttgccgaca gcgcggcgga
     1021 cattaaaaaa ctcagtttgg aactgggcgg caacgcgccg tttatcgtgt ttgacgatgc
     1081 cgatttggac aaagccgtcg aaggtgcgct cgccagcaag ttccgcaaca gcggtcagac
     1141 ctgcgtctgc accaaccgcg tttacgccca atccggcatt tacgacgaat tttgtcgcaa
     1201 attgagtgaa aaagtagccg agctcaaatt gggcaacggc ttggaggatg gcgtcaacca
     1261 agggccgctg attgaggaaa aagcggtaga aaaagtcgag cagcacattg ctgacgcgct
     1321 cgctaaaggc gcggtctgtc tgaccggcgg caaacgcagc gcattgggcg gaacgttttt
     1381 tgagccgacc gtcttaagcg gcgtaacggc gcaaatggcg gtggctcgcg aagaaacctt
     1441 cgggcctttg tgtccggtat tccgttttga aaccgaagcc gaagtcattg aggctgcgaa
     1501 caatacggaa tacggtttgg ctgcttacct ttttaccgcc gacaccgcac gccagtggcg
     1561 cgtcggcgaa gcattggaat acggtatggt cggcatcaat acgggcttaa tcagtaatga
     1621 agcggcaccg ttcggcggcg tgaaacgcag cggtttggga cgtgaaggca gcaaatacgg
     1681 tgcggacgaa tatctggaat tgaaatatct gtgtatggat gtcgggtgac ggatgccgtc
     1741 tgaacggcag ggtttcagac ggcgccgcat tttaagcagt ctctatctgt tgtacaatgc
     1801 gccctgtttt tacggttatt tttgatttga acaagatatg atggaaaacg gaaaaacagt
     1861 cccgagatgg cgttttgcct tgaaaagtgc gggctggcac ctcttaatca gcctgtcggt
     1921 tgcagggctg gcggcattgc tggtttttaa ggtttggtat ccttatcctt atgccgagct
     1981 gacgggaggg ctgtcgcttt atcagctggt ggtggctgtc gatattgtat gtggtccgct
     2041 gctgacttta attttggcaa gcccgaagaa aaagacaaag gcacgcatgg tcgatttttc
     2101 catggtcggc atcatccagc tggcggcttt ggtgtacggt ctgcacagcg tttctttggc
     2161 gcgccctgtg atagaggcgt ttgaaaaaga caggattaat gttgtgaccg ccgccgaagt
     2221 ggcggttgaa gatttgcaca aagcgccgga agggctgcaa agcctgtcgt ggttcggcat
     2281 ccgccgcatt gcattgaaag aacctgagga tgcggatgag aagaacaaga cgctggattt
     2341 gtccctgaaa ggtatcgagc cgagtatgcg tcccgaccgg tggctgccgt attccgacaa
     2401 ggaagcagaa aaaatccgca aacatctgaa accgctgaaa gtcttggcgg atgcgagaaa
     2461 aacgacggtt gcggacattc tgaaacaggc aggtctcgcc gaaggggagg agctgtatta
     2521 cctgccgttt accagcagca ggcagaaaga gtggatagtc attaccgata aagagggcaa
     2581 caccaaaggc tacgcgccga tagacggctt catcatcacc ccttaagcgt tgggactccg
     2641 tccgcactcg aacatccgtt cttcgcggcg gtagaatcag actgtatttg agaggggcgc
     2701 aacccctcaa tcagggcaac acatagggga aggctttatg tgccgtccgt gtgttgaaac
     2761 atttcaaaat aagctcatgc cgtccgaaca tcctttcaga cggtatggcg ttttgccatt
     2821 gccccgataa gctgttaaac tatttaaatt atttcgaccg aaggtacacg cacatgcaag
     2881 accccgagca gagcagcaag cccgcccgcc gttttttgtg cgtaccgtct gttcaggcag
     2941 cttgagcgct tcaggctgcc ttttcgcgtt tgaacttgag gaatacgaaa atcatggatt
     3001 tcagttggtt ggcagaaccg catacctgga taggttttgc cacgcttttg gtgttggaag
     3061 tcgtattggg gatagacaat cttgtctttg tggcgatttt ggcaaacaag gtccagcccg
     3121 cacggcgcga ccgcgcacgg attatcgggc tggggctggc agtcgtcatc cgcatcatta
     3181 tgcttgcttt tatggcgcac atcatcacgc tgaccgagcc gctgttccaa atcggcggcc
     3241 tcgccgtttc cggcaaggac atgattatgc tcgcgggcgg tattttcctg ctttacaaag
     3301 ccaccaccga actgcatgaa cgcctcgaag ggcacaaccg ttttactgtt gccgacaacc
     3361 aaaaaaaaca cgcgccgttt tggggcgtgg tcgcgcaaat cctgatactg gatgccgtgt
     3421 tttccatcga ttcggtcatt actgcggtgg cgatggtcga tcatatcgtc gtggcgatgg
     3481 gtgcggtcgt cgtcgcaatg gctgtaatga tttctgccag caaactcttg accgaatttg
     3541 tcgacagaca ccctaccgtc gtgatgctct gccttggttt tttgttgatg atcggtttca
     3601 gcctgattgc cgaagccttc catttccaca ttcccaaagg ctacctctac gccgccatcg
     3661 gcttctcgat tttaatcgaa ctgtttaacc agatttccca gcgcaacagc cgcaaaaacg
     3721 actacatcgg cagctcgtgg cgcaaacgga ctgctgaaaa tgtcttgggt atgatgggca
     3781 tacgcgaaag cgtgcttgcc gacgcgggcg gcgaatccgg ggacgacgcg cattttgaag
     3841 aaaacgaaaa atcgatgata cgcagcgtgc tgacgcttgc cgaacgcccg attatggggg
     3901 tgatgatccc acgccgcgac atcgaacggc tggacatttc ccaaagccgc gaagaacagt
     3961 atgcccaact gcaaaacacg ccttacagcc gcctgctcgt tgtcggaaag gcgggcgtgg
     4021 acgaaccttt gggctacatc aacaaaaaag acctgctgtc ccaactgctg gaaacaggcg
     4081 gtctcgacat tcagacggca ttgcgccagc cgctcgtcct acccgacggc acgaccgcct
     4141 tgggcgcgct cgaactcttc cgccaaagca gcgcggatta cgccctcgtc gtggacgagt
     4201 tcggcgcggt gttgggtatg gtgacgatga aagacctgtt ggaagccatt gccggcgact
     4261 ttcccgaaga attcgaacgc gaagaagaac cagccgttca ggggaatccc gatgaaagcc
     4321 tgacggtgga aggcgcgttg gaatatgtgg agctggcatc ccaactcaat ctgccgcagc
     4381 aggaggaaga tgccgatttc catacggttg ccgggctgat tatggaagaa ttgcaaacca
     4441 tccccgatgt cggtgatttt gccgatttcc acggctggcg gtttgaagtg gtcgaaaaag
     4501 aagggcagcg catcgagcgg gtcaaaatca ccaaattgcc cgaagaataa gtattcagga
     4561 tagaaaatga acgttttgat ttccaacgac gacggctacc tctccgaagg catcgccgtt
     4621 ttggcgcgcg ttacggcgga atttgccaac gtcagggtgg tcgcgcccga acgcgacagg
     4681 agcggggtca gcaattcgct gacgctggaa cgccctttgc agttgaaaca ggcgcaaaac
     4741 gggttctact atgtcaacgg cacgccgacc gactgcatcc acatcgggca gtctgtattt
     4801 tcggattttc aggccgattt tgtcttttcg ggcatcaacc ggggcgcgaa tatgggggac
     4861 gacacgcttt attcggggac ggttgcggcg gcaaccgaag cctaccttat gggcataccc
     4921 gccgtggcgt tttccttaaa cgacgcttcc ggacgctatt gggcgaccgc agaacaggca
     4981 ctgtggacat tgttggcgca ttttttcaaa acccccccgc agtcccctat tttatggaac
     5041 atcaatatcc ccgccgtcgc gccggaagat gtgcggggca ttaaaatcgc ccgtttgggc
     5101 aggagacacc acggtcagaa cgtcatcccc gcgcgcaatc cgcgcggcga acagattta
//
LOCUS       DAWHSK010000136         5135 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.136, whole genome shotgun sequence.
ACCESSION   DAWHSK010000136 DAWHSK010000000
VERSION     DAWHSK010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5135
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.136"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..535)
                     /locus_tag="WIC03_001438"
     CDS             complement(<1..535)
                     /locus_tag="WIC03_001438"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEZ3883919.1"
                     /translation="MALKTSNLEHAMLVHPEAMSVGALADKIRKIENWPQKGILFHDI
                     TPVLQSAEYFRLLVDLLVYRYMDQKIDIVAGLDARGFIIGAALAYQLNVGFVPIRKKG
                     KLPFETVSQSYALEYGEAAVEIHTDAVKPGSRVLLVDDLVATGGTMLAGLELIRKLGG
                     EIVEAAAILEFTDLQGGK"
     gene            663..1280
                     /gene="gmk"
                     /locus_tag="WIC03_001439"
     CDS             663..1280
                     /gene="gmk"
                     /locus_tag="WIC03_001439"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEZ3883920.1"
                     /translation="MSAYRKGNIFIISAASGTGKTTLVSRLLANHNGLRVSVSHTTRP
                     PREGEVNGVHYHFVSKEEFESLIAQEAFLEYADVFGNYYGTSAEGVNALAAAGYDVIL
                     EIDVQGAAQVRDALPEAVGIFILPPSFDVLAARLNGRGTDSREVIQRRLSKARHEIEQ
                     SVLFDFVVVNDDLARAEEDLRHIVNACRLKRSRQLGFIADLLENS"
     gene            1339..1545
                     /locus_tag="WIC03_001440"
     CDS             1339..1545
                     /locus_tag="WIC03_001440"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEZ3883921.1"
                     /translation="MARITTEDCTGKISNHFDLTLVAARRARQLENGNTPLVDDVRNN
                     KPTVTALREIAAGHIGTELLTRNK"
     gene            1637..3793
                     /locus_tag="WIC03_001441"
     CDS             1637..3793
                     /locus_tag="WIC03_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEZ3883922.1"
                     /translation="MPAPQPSSPYDPLTAEARALLFHTASYLKPEEQAELEKAVAYAF
                     RAHDGQTRKSGEPYITHPIAVATQLALWHMDIQGLCAGVMHDVLEDTGVTKGEMAAVF
                     GNTIAEMVDGLSKLEKLKFEDHAEHQAESFRKLILAMTKDVRVIVVKLADRLHNMRTL
                     GSMRPDKRRRIARETLEIYAQIANRIGLNNAYQELQDLSFQNLHPNRYETLKKAMDKS
                     RKNRQDVVGKVLRAFGQRLVGANIEAKIKGREKNLYGIHQKMMAKKLRFAEVMDIYGF
                     RVIVNSIPACYAALGALHTLYQPKPGRFKDYIAIPKSNGYQSLHTTLVGPYGLPIEVQ
                     IRTKEMDAVAEGGIAGHWSYKSYSKTVDQAVLHTNRWLKNILDLQASSANAIEFLEHV
                     KVDLFPNEIYILTPKGKILTLPKGATPVDFAYAVHTDIGHKTVAARINNIMMPLRTKL
                     KTGDSVEIITSEHAKPNPAWLNFAVSGRARSAIRQYIKNLNRHDAVVLGESLLQKALS
                     SLLPKDVLLSDGIKEKYLADLNDKQTSFEEVLYNVGMGHTLPVYVAMHIAELAGEHFG
                     SEVRLSSIKVDGQESGHIHFAECCHPVPGDSIRLLLVKGKGMIIHRDTCPTLLKSDPE
                     QQLDADWENMNGQNYRVGLQVQSEDSHGLLALMAQAISDSGADIESVETPSKSQSGTE
                     GFVEFKFLLKVKNLNQLNQIIQNLHSIPYIRKVIRS"
     gene            complement(3860..5044)
                     /gene="coaBC"
                     /locus_tag="WIC03_001442"
     CDS             complement(3860..5044)
                     /gene="coaBC"
                     /locus_tag="WIC03_001442"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEZ3883923.1"
                     /translation="MGKHILLGVTGSIAAYKSCELVRLLKKQGHSVTVVMSRSATEFV
                     SPLTFQALSSNPVLTDTHGSNGSNGMEHINLTRNADVFLIAPASMNTVAKICNGVADN
                     LLTNLAAARKCPLAIAPAMNVEMWLNPANQRNIAQLVSDGITVYMPGLGEQACGENGM
                     GRMPEPAELLDLLPDLWTPKILRGKKILITAGATFEAIDPVRGITNTSSGKMGIALAR
                     ACRAAGAEVSLIHGQLQTALPFGISDTVQAVSAEDMHRAVHRLIEKQDAFISVAAVSD
                     YKVKNKSTQKFKKDKNAKPLSIELDENPDILASIASLPNPPFCIGFAAETENVMAYAR
                     EKRIKKKIPVIVANDVSIAMGKPTNRITIIGDDGELSFPETSKDEAAMQIVERLAGYL
                     NK"
BASE COUNT         1321 a         1287 c         1288 g         1239 t
ORIGIN      
        1 tcttgccacc ttgaaggtcg gtaaattcca aaatggcggc ggcttcgaca atttccccgc
       61 cgagtttgcg gatcagctcc agtccggcaa gcatcgtgcc gcccgtggca accaaatcat
      121 cgaccagcag cacgcgcgaa ccgggtttga cggcatcggt gtggatttcc accgcagctt
      181 ccccgtattc gagcgcgtag ctttgcgata cggtttcaaa aggcagcttg ccttttttgc
      241 ggatggggac gaaaccgacg ttgagctggt aggcgagtgc cgcgccgata atgaagccgc
      301 gcgcgtccaa accggcaacg atgtcgattt tctgatccat atagcggtaa accaataaat
      361 caaccaaaag tcggaagtat tccgcgcttt gcaggacggg cgtgatgtcg tggaataaga
      421 tgcctttttg cggccagttt tcgattttgc ggattttgtc ggcaagcgcg ccgacactca
      481 tagcttcggg atgaaccagc attgcgtgtt ccaagtttga tgtttttaac gccatattgt
      541 agccgtcagc ctgccgcttg ccaaatcaat caaaacaata gcggcaaaat atgggcatcg
      601 gcaaatagga aaccgagtgt ccgttgtgat acaatcggct ggaaaatata caggtaaaaa
      661 atatgtccgc ttatcgaaaa ggcaacatct ttatcatttc ggccgcttcc ggcacgggca
      721 aaaccacgct ggtgtcgcgg ctgttggcaa accataacgg tttgcgcgtt tccgtatcgc
      781 acacgacgcg tccgccgcgt gaaggcgaag taaacggcgt acattatcac tttgtttcca
      841 aagaagagtt tgagtcgctt atcgcacagg aggctttttt ggaatacgca gacgtattcg
      901 gcaactatta cggcacaagc gcggagggcg tgaatgcgtt ggcggcggca ggttatgacg
      961 tgattttgga aatcgacgtt cagggcgcgg cgcaggttcg agatgcgctg cctgaagctg
     1021 tcggcatctt tatcctgccg ccgtctttcg atgtgcttgc tgcacgtctg aacggacgcg
     1081 ggacggacag tcgggaagtt atccaaagga ggctgtcgaa ggcaaggcat gaaatcgaac
     1141 agtccgtatt gttcgatttt gtcgtggtca atgatgactt ggcgcgggcg gaggaggatt
     1201 tgcgccatat tgtgaatgcc tgccgtctga aaaggtcgcg gcaactggga tttattgcag
     1261 atttgttgga aaattcctaa aaaaccgcga aaatacccag tttcctattt taaatatttt
     1321 tgaaagaaag caaataatat ggcacgtatt accaccgaag actgtaccgg aaaaatttcc
     1381 aaccattttg acctgacatt ggtagcggcc cgtcgggccc gccagcttga aaatggcaat
     1441 acgccgcttg tggacgatgt ccgcaataac aaaccgaccg ttaccgcctt aagggaaatc
     1501 gccgccgggc atatcggtac ggaactgttg acgcgcaata aataaattct gccggaaacg
     1561 cacgtcggca cattctgccg ccgtgcagtc cgacggtttg aaatgaaaaa cacatacata
     1621 ccgaaagccg tctgaaatgc ccgcccccca accttcctcc ccttacgacc ccctgaccgc
     1681 cgaagcgcgt gccctgcttt tccataccgc ctcctacctc aagcccgagg aacaggcgga
     1741 gcttgaaaaa gctgtcgcct atgcgtttcg tgcccacgac gggcaaaccc gcaaaagcgg
     1801 ggagccctac atcacgcatc cgattgccgt tgcgacgcag ctcgcccttt ggcatatgga
     1861 catacagggt ctttgtgcag gcgtgatgca cgacgtattg gaagatacgg gcgtgacaaa
     1921 aggggaaatg gcggcggtgt tcggcaatac gattgccgag atggtggacg ggctgtccaa
     1981 actcgagaaa ctcaaatttg aagatcatgc ggaacatcag gcggagagtt tccgcaaact
     2041 gattttggcg atgaccaaag atgtgcgcgt catcgtcgtc aaacttgccg accgcctgca
     2101 caatatgcgg acgctcggtt cgatgcgccc ggacaaacgc cgccggattg caagggaaac
     2161 ccttgaaatc tatgcacaga ttgccaaccg tataggtttg aataacgcat atcaagagct
     2221 tcaggattta tcgttccaaa acctgcatcc caaccgctac gagactttaa aaaaagcgat
     2281 ggacaagagc cggaagaacc ggcaggacgt tgtcggcaaa gtgttgcgcg cattcggcca
     2341 gcggctggta ggcgcgaata tagaggccaa aatcaaaggc agggaaaaaa acctgtacgg
     2401 catccatcag aaaatgatgg cgaaaaagct gcgctttgcc gaggttatgg atatttacgg
     2461 tttccgcgtc attgtcaaca gcattccagc ctgttatgcc gcactcggcg cattgcacac
     2521 cctctatcag cccaagcccg ggcggttcaa agattatatc gccattccga aaagcaacgg
     2581 gtatcaaagt ctgcatacga ctttggtcgg tccttacggc ttgccgattg aagttcagat
     2641 acgtaccaag gaaatggatg ctgttgccga aggtggaatc gccggacatt ggagctataa
     2701 atcatattct aagacggtcg atcaggcggt gcttcacaca aaccggtggc tgaaaaatat
     2761 cttagatttg caggcaagca gtgccaatgc cattgagttt ctcgaacacg tcaaagtcga
     2821 tttgtttccg aacgaaatct acatccttac gccaaaagga aaaatcctaa ctttgcccaa
     2881 aggggcaacg cctgtcgatt ttgcttatgc ggtgcatacc gatatcgggc acaaaaccgt
     2941 tgccgcacgt atcaacaata tcatgatgcc gttgcgtacg aagctcaaaa ccggcgattc
     3001 cgtcgaaatt attacatccg aacatgccaa acccaatcct gcatggttga atttcgccgt
     3061 gtcaggcagg gcgcgcagtg ccatacgcca atatattaaa aaccttaacc ggcacgatgc
     3121 ggtcgttttg ggagagagcc tcttacaaaa agccctgtcc agtttgctgc ccaaagatgt
     3181 cctgctttca gacggcatca aagaaaaata ccttgccgat ctcaacgaca aacagacttc
     3241 atttgaggaa gtgctgtaca acgtcggtat ggggcatacc ctgcctgttt atgtcgccat
     3301 gcacattgcc gagttggcag gggagcattt cggcagcgag gtcaggctca gttccattaa
     3361 agttgatggt caggaaagcg ggcatattca ttttgcagag tgctgccacc ctgttcctgg
     3421 tgattccatc cgtttgctgt tggttaaggg aaaaggcatg attatccaca gagatacctg
     3481 cccgacgttg ttgaagtccg atcccgaaca gcagctggat gcagactggg aaaatatgaa
     3541 cgggcagaac taccgtgtcg ggcttcaagt ccaatcggaa gacagccacg gcctgttggc
     3601 attaatggcg caagcgattt ccgattccgg tgcggacatt gagtcggtcg aaacaccgtc
     3661 taaatcccag tcgggaacgg aaggttttgt cgaattcaaa ttcttattga aagtcaagaa
     3721 tctgaatcaa ttgaatcaga ttattcaaaa tctgcatagt attccatata tccgcaaagt
     3781 catcagaagt tgaagcaggt aaaacgggtt gccttttgat taacaggcaa cccgctttat
     3841 ggtttatccg ttcaattgct tatttgttca aatatccggc aagcctttca acaatctgca
     3901 tcgccgcttc atctttactt gtttcgggaa aagatagttc cccgtcgtcc ccgataatgg
     3961 taatccggtt ggtcggtttg cccatcgcga ttgaaacatc attggcaact atcaccggta
     4021 tttttttctt aatacgtttt tctcgtgcat atgccattac attctccgtt tcagcggcaa
     4081 aaccgatgca gaacggcggg ttcggcaatg aggcaataga agccaaaata tcggggttct
     4141 catccaattc gatggataac ggtttggcat ttttatcttt tttgaatttt tgcgtactct
     4201 tgtttttaac cttatagtct gagacggcgg caacagaaat aaaagcatct tgtttttcaa
     4261 tcaaacgatg cactgcgcga tgcatatctt cagcgctgac ggcttgaacc gtatcggata
     4321 tgccgaaagg cagcgcggtt tgaagctgtc cgtgaatcag gctgacttcc gcaccggcgg
     4381 cacggcacgc ccgcgccaaa gctatcccca tttttccgct ggaggtattt gtgatgcccc
     4441 tgacagggtc aatggcttca aatgtcgcac ctgcggtaat caagattttc ttgcccctta
     4501 aaattttcgg tgtccacaag tccggaagca aatccagcaa ttcggcaggt tccggcatcc
     4561 ttcccatacc attttctccg caagcctgtt cgcccaagcc cggcatatag acagtaatgc
     4621 cgtctgaaac cagttgtgcg atattccgtt ggttggcagg gttgagccac atttccacat
     4681 tcatcgcggg agcgatggca agaggacatt tccgtgcggc tgccagattg gtcagtagat
     4741 tatctgccac gccgttacag atttttgcca cggtattcat acttgccggc gcaatcagaa
     4801 aaacatccgc attccgggtc aggttgatat gttccatgcc gtttgaaccg ttgctgccgt
     4861 gcgtgtcggt cagaacggga ttgctgctca aagcctgaaa agtcagcgga gaaacaaatt
     4921 cagttgccga gcggctcata accaccgtaa ccgaatgccc ctgttttttc agcagtcgca
     4981 ccaactcgca agacttatac gccgcaatac tgcccgttac acctaaaaga atatgtttgc
     5041 ccattttgac aatttctaat tacgaagaaa aacagcagcc attctataac aaagtacgga
     5101 tacattagtt accgttttgg atacaaaatg ccgtc
//
LOCUS       DAWHSK010000137         5132 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.137, whole genome shotgun sequence.
ACCESSION   DAWHSK010000137 DAWHSK010000000
VERSION     DAWHSK010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5132
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.137"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..2336)
                     /locus_tag="WIC03_001443"
     CDS             complement(<1..2336)
                     /locus_tag="WIC03_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3683 domain-containing protein"
                     /protein_id="HEZ3883924.1"
                     /translation="MTTTTAPQRIREIPYNYTSYTDREIVIRLLGDEAWQILQDLRGQ
                     RKTGRSARMLFEVLGDIWVVVRNPYLVDDLLEHPKRRAALVREMRHRLNEIRKRRDDN
                     RQVDVLVAAADKAVGRFDSSFDETSQKRRQILARLSKITKPHNIMFDGLARVTHVTDA
                     TDWRVEYPFVVVNPDTEAEIAPLVRALIELDLVIIPRGGGTGYTGGAIPLDAMSAVIN
                     TEKLDKHRGVEFVELAGLDGKHPIIHCGAGVVTRRVEETAHQAGLVFAVDPTSADASC
                     VGGNVAMNAGGKKAVLWGTALDNLAYWQMVNPQGEWLRIERVRHNFGKIHDEETAIFD
                     VHTLDSDGLNVVKTERLEIPGHKFRKVGLGKDVTDKFLSGLPGVQKEGTDGIITSVAF
                     VLHKMPKYTRTVCMEFFGTVATATPSIVEIRDFLLAHESVRLAGLEHLDWRYVRAVGY
                     ATKAAGKGRPKMVLLADVVSDDEAAVEAAAEHICELARARDGEGFIAISPEARKTFWL
                     DRSRTAAIAKHTNAFKINEDVVIPLERLGEYSDGIERINIELSIQNKLKLCAALEQYL
                     SGKLPIDKMGTDLPTAELLGERGKHALAHVSAVKARWDWLLANLDTPLADYKARYGAA
                     VHAAPEAKDNESCFTAFRDFRLRVSVKADVMKPLSEIFSGKTDTKIIEGLGKIHAKTV
                     RGRVFVALHMHAGDGNVHTNIPVNSDDAEMLQTAYRSVERIMKIARSLNGVISGEHGI
                     GITKLEFLSDEEMQPFWDYKNQVDPKHTFNRHKLMKGS"
     gene            2597..2693
                     /gene="ffs"
                     /locus_tag="WIC03_001444"
     ncRNA           2597..2693
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="WIC03_001444"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            2861..4552
                     /gene="dld"
                     /locus_tag="WIC03_001445"
     CDS             2861..4552
                     /gene="dld"
                     /locus_tag="WIC03_001445"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HEZ3883925.1"
                     /translation="MSASQLLSRLTQTVGEKYIITDPAKTEQYRQGYRFGEGRALAVV
                     RPGTILEMWKILQACVEADVIVITQAANTGLTGGSTPDGNDYDRDIVIVNTMRMNIIQ
                     TINNNEQVVCLPGSTLNQLELLLKPLGREPHSVIGSSCIGASVLGGVCNNSGGALVQR
                     GPAYTEMALFAQINEEGRLELVNHLGIDLGDTPEEILTNLQGHHYQNKDIKQDAGKGH
                     DHAYCEHVRQVDEPTAARFNADPARHYEASGCAGKLMVFAVRLDTFPQEKQTAVFYIG
                     TNDINELTDIRRAALGEFESLPVSGEYIHRHAFDIADVYGKDTFYVIKKFGTHQLPKL
                     FDLKARVDRFGKKASFLPKHFSDKAMQFVSKYLPDHLPKSMRDYRDKYEHHLILKMGG
                     KGVDEARAFLKEYFAHHSGAFFECNAEETQAAMLHRFAVASAAIRYRAVHDDEVEDLV
                     ALDIALRRDDRDWFEKLPSEIDNKIIHKLYYGHFMCHVFHQDYIIKKGNDCMALEHEM
                     LHLLDQRGAQYPAEHNVGHLYEAKPALKQFYRKLDPTNSFNPGVGKTSKKKNWAE"
     gene            4684..4791
                     /locus_tag="WIC03_001446"
                     /pseudo
     CDS             4684..4791
                     /locus_tag="WIC03_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            4847..>5132
                     /locus_tag="WIC03_001447"
     CDS             4847..>5132
                     /locus_tag="WIC03_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HEZ3883926.1"
                     /translation="MKPYKIYTHPALPPQAVKQGWSWPGLLFGTLWACFKRMWGLGLG
                     LTGAIFVLAVFAQLVYGDTPATDSAFNVLGLAVSVWFGAKGNSLYARHLLS"
BASE COUNT         1184 a         1419 c         1384 g         1145 t
ORIGIN      
        1 tccgagcctt tcatcagttt gtggcggttg aaggtgtgtt tgggatcgac ttggtttttg
       61 tagtcccaaa acggctgcat ttcttcgtcg cttaaaaatt cgagcttggt aatgccgatg
      121 ccgtgttcgc cggaaatcac gccgttaagc gaacgggcga ttttcataat gcgttccaca
      181 gaacggtatg ccgtctgaag catttcggca tcgtctgaat tgacggggat attggtgtgg
      241 acattgccgt cgccggcgtg catatgcagg gcgacaaaga ctctgccgcg cacggttttg
      301 gcgtggattt tgcccaagcc ttcgatgatt ttggtgtcgg ttttgccgct gaagatttca
      361 gaaagcggct tcattacgtc tgctttgaca gacacgcgca ggcggaaatc gcggaatgcg
      421 gtaaagcagc tttcattgtc tttggcttcg ggcgcggcgt ggacggctgc gccgtagcgg
      481 gccttgtagt cggcaagcgg cgtgtcgaga ttggcgagca accagtccca acgcgctttg
      541 acggcggaaa cgtgggccag ggcgtgtttg ccgcgttcgc ccaacagttc ggcggtcggc
      601 aggtcggttc ccattttgtc gatggggagt ttgccggaca gatattgctc caaggcggca
      661 cagagtttga gtttgttttg aatggaaagc tcaatgttga tgcgttcgat gccgtctgaa
      721 tactcgccga gcctttcgag tgggatgacc acgtcttcgt tgattttaaa ggcgttggtg
      781 tgtttggcga tggcggcggt gcggctgcgg tcgagccaga aggttttgcg ggcttcggga
      841 gatatggcga taaagccttc gccgtcgcgg gcgcgggcaa gttcgcagat gtgttcggcg
      901 gctgcctcta cggcggcttc gtcgtctgaa accacgtccg ccagcaaaac cattttcggc
      961 cgtcccttgc ccgccgcttt ggtggcgtag ccgacggcgc ggacataacg ccagtccaaa
     1021 tgttccaaac ctgccagccg tacgctttca tgggcaagca aaaagtcgcg gatttcgaca
     1081 atagatggcg tggcggtggc aaccgtgccg aaaaactcca tacacacggt gcgcgtgtat
     1141 ttcggcattt tatgcaacac gaaggcaacg ctggtgatga tgccgtccgt gccttctttc
     1201 tgcacgccgg gcaggccgct caagaatttg tcggtaacgt ctttgcccaa accgactttg
     1261 cggaatttgt gaccgggaat ttccaagcgt tcggttttga cgacattgag gccgtctgaa
     1321 tccagtgtgt gaacgtcaaa tattgcggtt tcttcatcgt ggattttgcc gaaattgtgg
     1381 cgcacgcgct cgatacgcaa ccattcgcct tgagggttga ccatctgcca gtaggcgagg
     1441 ttgtccaacg ccgtccccca cagcacggct tttttaccgc ctgcgttcat tgctacattg
     1501 ccgcccacgc aggacgcatc ggcggaagtc ggatcgacgg cgaacaccaa acctgcctga
     1561 tgcgcggttt cttccacccg ccgcgttacc acgcccgcgc cgcaatggat aatcggatgt
     1621 ttgccatcca agcccgccag ttcgacaaac tccacgccgc gatgtttgtc cagcttttcg
     1681 gtattgatga ccgcgctcat tgcgtccaaa ggaatcgcgc cgccggtata acccgtgccg
     1741 ccgccgcgcg ggataataac caagtccaat tcgattaaag cgcgcaccaa aggcgcaatc
     1801 tctgcctcgg tatcaggatt gacgacgaca aacggatact ccacgcgcca gtcggtcgca
     1861 tcggtaacgt gcgttacgcg cgccagcccg tcaaacataa tattgtgcgg cttggtgatt
     1921 ttgctcaaac gcgccaaaat ctgccgccgc ttttggctgg tttcatcaaa actgctatca
     1981 aaacgcccga ccgctttgtc cgccgccgca accaagacat ccacttgccg attgtcgtcg
     2041 cggcgtttgc ggatttcgtt caggcggtgg cgcatttcgc gtaccaacgc ggcgcggcgt
     2101 ttcggatgct ccaacaagtc atcgaccaga tatggattgc gcacaactac ccaaatatcg
     2161 cccaacactt caaacagcat ccgtgccgaa cgtccggttt tacgctgtcc gcgcaagtct
     2221 tgcagaatct gccacgcttc gtcgcccagc aggcggatga cgatttcgcg gtcggtataa
     2281 gaagtatagt tatagggaat ttctcgaata cgctgagggg cagtagtcgt ggtcatggtg
     2341 tgtccgtgct tggcagtttt tatttgaatg ccgtctgaaa attcggtttc aggcgagttt
     2401 gaaaaggcga taatgtagtt caatttttga tatttttcaa tgccgaaaca ggaaataccc
     2461 ttatagaaac aaaataacgc tttgagaaat tgttgacaat attatcaaat ataaatttta
     2521 ggtggtgttt ccggcgtatg accgtatcaa aacggttgag tttttcagaa aacccgttat
     2581 aatgccgttt ctctgaggcg ggtctccccg catggcaaat cggaacaccg ggtcaggggc
     2641 ggaagccagc agcccactcc ggtgcgccag tgccgggggt ttggcccgcc gccctatttg
     2701 aaacgccgga gctttgatgt tccggcgttc ccgtatcgca ccgatgccgt ctgaaagccg
     2761 ttcagacggc attttcttta ctttgccata acgtataact acaatgggaa aacaatttca
     2821 atgtgcatat gcacctgccg acaattttaa ggaaactgcc atgagcgcat cacaattact
     2881 gtcaagactg acccaaaccg taggcgaaaa atacatcatc accgaccccg cgaagaccga
     2941 acaataccgc caaggctacc gcttcggcga gggcagggcg ttggcggtgg tgcgccctgg
     3001 aaccattctg gaaatgtgga aaattctgca ggcctgcgtc gaagcggacg tgattgtgat
     3061 tacgcaggcg gcgaataccg gtttgacggg cggctcgacc cccgacggca acgattacga
     3121 ccgcgacatc gtgattgtga acaccatgcg gatgaacatc atccaaacca tcaacaacaa
     3181 cgaacaagtc gtctgtctgc ccggttcgac cctgaaccag cttgaattgc tgctgaaacc
     3241 tttggggcgc gaaccgcatt cggtcatcgg ctcatcctgt atcggcgcgt ccgtcttggg
     3301 cggcgtgtgc aacaactccg gcggcgcgtt ggtacagcgc ggcccggcct acaccgaaat
     3361 ggcgttgttc gcccaaatca acgaagaagg caggttggaa ctggtcaacc acttgggcat
     3421 agacttgggc gacacgcccg aagaaatcct gaccaacctg caaggtcatc attatcaaaa
     3481 caaagacatc aaacaagacg cgggcaaagg acacgaccac gcctattgcg aacacgtccg
     3541 ccaagtggac gaaccgaccg ccgcgcgttt caatgccgac cccgcccgcc attacgaagc
     3601 ctcgggctgc gcgggcaaac tgatggtttt tgccgtccgt ttggacacct tcccgcaaga
     3661 aaaacaaacc gccgtgttct acatcggcac gaacgacatc aacgagctga ccgacatccg
     3721 ccgcgccgcc ttgggcgaat ttgaaagcct gcctgtttcc ggcgaataca tccaccgcca
     3781 cgctttcgat attgccgacg tgtacggcaa agacacgttt tacgtcatca aaaaattcgg
     3841 tacgcaccaa ctgccgaaat tatttgactt gaaggcgcgc gtggacaggt tcggcaaaaa
     3901 agccagcttc ctgcccaaac atttttccga caaggccatg cagttcgtca gcaaatacct
     3961 gcccgaccac ctgcccaaat ccatgcgcga ttaccgcgac aaatacgaac accacctgat
     4021 tctgaaaatg ggcgggaaag gcgtggatga ggcgcgcgcg ttcttaaaag aatattttgc
     4081 acaccacagc ggcgcgtttt tcgagtgcaa cgccgaagaa acccaagccg cgatgctgca
     4141 ccgtttcgcc gtcgcctccg ccgccatccg ctaccgcgcc gtgcatgatg acgaagtgga
     4201 agacttggtc gcgctcgaca tcgccctgcg ccgcgacgat cgcgactggt ttgaaaaact
     4261 gccgtcggaa atcgacaata aaatcatcca taaactctac tatggacatt tcatgtgcca
     4321 cgttttccat caggattaca tcatcaaaaa aggcaacgac tgcatggcgt tggaacacga
     4381 aatgctgcat cttttagacc aacgcggcgc gcaatatccc gccgaacaca acgtcggtca
     4441 tttgtatgaa gccaaacccg cgctcaaaca gttttaccgc aaacttgacc cgaccaacag
     4501 cttcaacccg ggcgtaggca aaaccagcaa aaagaaaaac tgggcggaat aagcgcgtcc
     4561 gctttgaagg caggcaatgc cgtctgaagg caaaacgccg ttcagacggc attgtcgggt
     4621 ttgaagttcc ccatcgggta atcgctttca agccgtatgc cggcacaggc gcgcgcgcct
     4681 cccaaatata gtggattaaa tttaaatcag gacaaggcaa cgaagccgca gacagtacag
     4741 atagtacggc aaggcgaggt aacgccgtac tggtttaaat ttaatccact ataaagtctg
     4801 atcctacctt cccaaagggc agggtttcaa ccgaaaagga aacacgatga aaccatacaa
     4861 aatctacacc catcccgccc tgccgcccca agccgtcaaa caaggctggt cgtggccggg
     4921 ccttttgttc ggcacgctgt gggcgtgttt caaaagaatg tggggtttgg ggctggggct
     4981 gacgggcgcg atattcgtgc tggcggtgtt tgcccaactg gtttacggcg acacgcccgc
     5041 cacagactcg gcgtttaacg tattgggctt ggcggtttcc gtctggttcg gcgcaaaggg
     5101 caacagcctt tatgcacgcc accttttatc gc
//
LOCUS       DAWHSK010000138         5121 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.138, whole genome shotgun sequence.
ACCESSION   DAWHSK010000138 DAWHSK010000000
VERSION     DAWHSK010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5121
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.138"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            164..1276
                     /gene="nadA"
                     /locus_tag="WIC03_001448"
     CDS             164..1276
                     /gene="nadA"
                     /locus_tag="WIC03_001448"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEZ3883927.1"
                     /translation="MQTAARRSFDYDMPLIQTPTSACQIRQAWAKVADTPDRETADRL
                     KDEIKALLKEKNAVLVAHYYVDPLIQDLALETGGCVGDSLEMARFGAEHEAGTLVVAG
                     VRFMGESAKILCPEKTVLMPDLEAECSLDLGCPEEAFSAFCDQHPDRTVVVYANTSAA
                     VKARADWVVTSSVALEIVSYLKSRGEKLIWGPDRHLGDYIRRETGADMLLWQGSCIVH
                     NEFKGQELAALKAEHPDAVVLVHPESPQSVIELGDVVGSTSKLLKAAVSRPEKKFIVA
                     TDLGILHEMQKQAPDKEFIAAPTAGNGGSCKSCAFCPWMAMNSLGGIKYALTSGRNEI
                     LLDRKLGEAAKLPLQRMLDFAAGLKRGDVFNGMGPA"
     gene            1328..2836
                     /gene="nadB"
                     /locus_tag="WIC03_001449"
     CDS             1328..2836
                     /gene="nadB"
                     /locus_tag="WIC03_001449"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HEZ3883928.1"
                     /translation="MQTDCDVLIAGNGLAALTLALSLPESFRIVILCKNRLDDTASRH
                     AQGGIAAAWSGEDDIEKHVADTLEASAGLCDEAAVRTILSQGKPAIEWLLAQGVAFDR
                     NYDGLHLTREGGHTCRRIAHVADYTGEAVMQSLLAQIRRRPHIRVCERQMALDIQTES
                     GVACGLIVLDRRTQETYLIRARHTVLAGGGLGQIYAATTTPPECTGDAIAMAIRAGCA
                     VENLEFIQFHPTGLARSSENGRTFLISEAVRGEGGILTNQAGERFMPHYDRRAELAPR
                     DIVARAIAAEIAKQTPDFVSLDISRQPEAFVRRHFPSIHRHCLSQCGLDITRQAIPVR
                     PVQHYTCGGIQTDPCGRTSLPQLYALGETACTGLHGANRLASNSLLECVVTARLAAQT
                     IADGQAFQAKTSPRPSENPSAEAGIFADDLQNTFSRPVLQTFNQRHLGILRSDTGLRR
                     AIAQLRLWKQNQAEPHTASEYENRNLLECSLAVAQAAYRRRQNIGAHFNSDC"
     gene            complement(3012..3677)
                     /gene="pgmB"
                     /locus_tag="WIC03_001450"
     CDS             complement(3012..3677)
                     /gene="pgmB"
                     /locus_tag="WIC03_001450"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEZ3883929.1"
                     /translation="MTFTAVLFDLDGVITDTAEYHYRAWKKLAEELGIGIDRKFNEQL
                     KGVSRDDSLKRILAHGGKTVGEAEFAELTRRKNDNYVEMIQAVKPEDVYLGILPLLEA
                     LKANGKKIALASASKNGPFLLERMGLTHFFDTVADPAAVAHSKPAPDIFLAAAEGVGA
                     DIRRCIGIEDAAAGVAAIKAAGALPIGVGKAEDLGSDIALVSDTAELTYAYLQNVWAQ
                     SGR"
     gene            complement(3690..>5121)
                     /locus_tag="WIC03_001451"
     CDS             complement(3690..>5121)
                     /locus_tag="WIC03_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEZ3883930.1"
                     /translation="SEKVAGVAFETLLDAHKAGWLHRWEIADVVIEGNDEAQQGIRFN
                     LFQLFSTYYGEDARLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAEPEVTRNLLQY
                     RRNQLPQAQHNAREQGLAGALYPMVTFTGIECHNEWEITFEEIHRNGAIPYAIYNYTN
                     YTGDESYLAKEGLEVLVEVSRFWADRVHFSKRSGKYMIHGVTGPNEYENNINNNWYTN
                     TLAAWVLDYTREALAKYPRPDLNVSAAELEKWADISANMYRPHDEELGVFVQHDGFLD
                     KDIRPVSALSPDDLPLNQKWSWDKILRSPFIKQADVLQGIYFFGDRFNIDEKRRNFDF
                     YEPMTVHESSLSPCIHSILAAELGKEEKAVEMYRRTARLDLDNYNNDTEDGLHITSMT
                     GSWLAIVQGFAQMKTWGGKLSFAPFLPSAWTGYTFHINYRGRLIKVAVGKENVVFTLL
                     KGEPLDLQVYGKDITLNGSHTAALEK"
BASE COUNT         1055 a         1462 c         1504 g         1100 t
ORIGIN      
        1 caaagcggtt tgtcggaacg aagcgggtcg gttttacggt atcgcaaacc tgttttcaga
       61 cgaccttgct tattatactc aatttgagca taatataaaa atatttcaaa atataaacgg
      121 caaatcttta tgccgtctga aaaaccagcc ataaggaagt ctcatgcaaa ccgccgcccg
      181 ccgctcgttc gactacgata tgccactcat ccaaacgccg acttccgcct gccaaatccg
      241 tcaggcgtgg gcgaaggttg ccgacacgcc cgaccgcgag acggcagatc gtctgaaaga
      301 cgaaatcaag gctttgctga aggagaaaaa cgcggtcttg gtggcgcatt attatgttga
      361 tccgctgatt caggatttgg ctttggaaac gggcggatgc gtgggcgatt ctctggaaat
      421 ggcacgcttc ggcgcggaac acgaagccgg tacgctggtg gtggcgggcg tgcgtttcat
      481 gggagagagc gcgaaaatcc tctgccctga aaaaacggtg ctgatgcctg atttggaagc
      541 ggaatgttct ttggatttgg gttgcccgga agaggcgttt tcggcgtttt gcgaccaaca
      601 ccccgaccgc acggtggtgg tgtacgccaa cacttccgcc gccgtgaaag cgcgtgccga
      661 ttgggtggta acgtcttcgg tggcgttgga aatcgtgtcg tatctgaaat cgcgcggcga
      721 gaagctgatt tgggggcccg accgccacct cggcgactac atccgccgcg aaacgggcgc
      781 ggatatgctg ttgtggcagg gttcgtgcat cgtccacaac gaatttaaag ggcaggagct
      841 ggcggcgttg aaggcggaac accccgatgc ggtggtgctg gttcatcccg aatcgccaca
      901 aagcgtcatc gaactgggcg acgtggtcgg ctcgaccagc aaactgctga aagctgccgt
      961 atcacgcccc gaaaaaaaat tcatcgtggc gaccgatttg ggcatcctgc acgaaatgca
     1021 aaagcaggcg cccgacaaag aatttatcgc cgcgccgacg gcgggcaacg gcggaagttg
     1081 caaaagctgc gcgttctgcc cgtggatggc gatgaattcg ctgggcggca tcaaatacgc
     1141 cctgacaagc ggacgtaacg aaatcctgtt ggacaggaag ctgggcgaag ccgccaaact
     1201 gcctttgcag cgtatgctcg acttcgcggc aggactcaaa cgcggggatg tgttcaacgg
     1261 catggggccc gcctgatttg ccgtccatat tgccgtttca gacgacctct ccaacaagga
     1321 caacaccatg caaaccgatt gcgacgtatt gattgccgga aacgggctgg cggcactgac
     1381 gctcgccctg tcgctgcctg aatcgttccg catcgtcatt ttgtgcaaaa accggctgga
     1441 cgacaccgcc agccgtcatg cgcaaggcgg gattgcggcg gcgtggtcgg gagaggacga
     1501 catcgaaaaa cacgtcgccg atactttgga agcgagcgca ggtttgtgcg acgaagccgc
     1561 cgtccgcacc atcctgtcgc agggcaaacc ggcaatcgaa tggctgctgg cgcagggcgt
     1621 ggcgttcgac cgaaattatg acggcctgca cctgacgcgc gaaggcgggc acacctgccg
     1681 ccgaatcgcc cacgtcgccg actacacggg cgaagccgtc atgcagagcc tgcttgccca
     1741 aatacgccgc cgcccgcaca tccgcgtttg cgagcggcag atggcgttgg acattcaaac
     1801 cgaatcaggt gtggcgtgcg gactgatcgt ccttgaccgc cgaacgcaag aaacctacct
     1861 catccgcgcc cgccataccg tactcgcagg cggcggcttg ggacagattt acgccgccac
     1921 caccacgccg cccgaatgca cgggcgacgc catcgccatg gcgatacgcg caggctgtgc
     1981 agttgagaac ctcgaattta tccaattcca ccccacaggc ttggcaagat cgtctgaaaa
     2041 cggccgtacc ttcctgattt ccgaagccgt gcgcggcgaa ggcggcatcc tgaccaacca
     2101 agcgggcgaa cggtttatgc cgcattacga ccgccgcgcc gaactcgcgc cgcgcgacat
     2161 cgttgcccgc gccatcgccg ccgaaatcgc caaacaaact ccagacttcg tctcgctcga
     2221 catcagccgc caacccgaag cgttcgtccg ccggcatttt ccgtccatcc atcggcactg
     2281 tttatcccaa tgcggcctgg acatcacgcg ccaagccatc cccgtccgcc ccgtgcaaca
     2341 ctacacctgc ggcggcatcc aaaccgaccc ctgcggcaga acctccctgc cgcagctcta
     2401 cgccttaggc gaaaccgcct gcaccggctt gcacggagcc aaccgcctcg ccagcaactc
     2461 cctgctcgaa tgtgtcgtta ccgccagact tgccgcccaa accatcgcgg acggacaagc
     2521 attccaagcc aaaacgtctc caaggccgtc tgaaaacccc tccgccgaag caggcatctt
     2581 tgcagacgac cttcaaaaca cattcagccg ccccgtcctg caaacgttca accaacgcca
     2641 tctcggcatc ctacgcagcg ataccggcct gcgccgcgcc atcgcccaac tgcggctttg
     2701 gaagcaaaac caagccgaac cgcacaccgc gtccgaatac gaaaaccgca acctgctcga
     2761 atgcagcctc gccgtcgccc aagccgcata caggcggaga cagaacatcg gcgcgcattt
     2821 taatagtgat tgttaaggtt ggctggttag aaaaaatcgt tcaaatgaat tttaatagca
     2881 tggttaagag attggcattc agtttttagg gttgcgtaag ttgggttatc aatctgttct
     2941 aaaagcagaa aggccgtctg aaacactgtt cagacggcct tttacttcct tgacacttta
     3001 tctgacgcgt tttacctgcc cgactgtgcc cacacgtttt gcaggtaggc gtaggtcagc
     3061 tcggcggtat cggacaccag cgcgatgtcg ctgcccaagt cttcggcttt gcccacgccg
     3121 ataggcaagg cgccggcggc tttgatggcg gcgacacctg cggcagcgtc ttcaatgccg
     3181 atgcagcggc ggatgtccgc gcccacgccc tcggcggcgg cgaggaagat gtcgggggcg
     3241 ggtttggaat gcgcgacggc ggcagggtcg gcgacggtgt cgaagaagtg ggtcagcccc
     3301 atgcgttcga gcaggaaggg gccgttttta ctggcggacg caagggcgat ttttttgccg
     3361 tttgctttta atgcttccag cagcggcaaa atgccgagat acacgtcttc gggtttgact
     3421 gcctgaatca tctcgacgta gttgtcgttt ttgcggcggg tcagttcggc gaactcggct
     3481 tcgccgacgg ttttgccgcc gtgtgcgagg atgcgtttga gcgaatcgtc gcgcgacacg
     3541 cctttgagct gctcgttaaa cttgcggtct atgccgatgc ccagttcttc ggcgagcttt
     3601 ttccatgcgc ggtagtggta ttcggcggtg tcggtgatga cgccgtcgag atcgaagagc
     3661 actgcggtaa acgtcatttt gcgccctcct tatttttcca acgcggcggt atggctgccg
     3721 ttgagtgtga tgtctttgcc gtacacctgc aaatcgagcg gctcgccttt gagcagggtg
     3781 aagacgacgt tttctttgcc gacggcgact ttaatcagac ggcctcggta gttgatatgg
     3841 aaggtgtagc ctgtccacgc gctcggcagg aacggtgcga agctgagttt accgccccaa
     3901 gttttcattt gggcgaaacc ttggacgatg gcgagccacg agccggtcat agaagtaatg
     3961 tgcaggccgt cttcggtgtc gttgttgtag ttgtccaagt ccaggcgggc tgtgcgccgg
     4021 tacatttcca cggctttttc ttctttgccc agttcggcgg cgagaataga gtggatacac
     4081 ggcgacaacg agctttcgtg cacggtcatc ggttcgtaga agtcgaaatt gcggcgtttt
     4141 tcgtcgatat tgaaacggtc gccgaagaaa tagatgcctt gcaagacatc cgcctgtttg
     4201 atgaagggcg aacgcaggat tttgtcccac gaccatttct ggttgagcgg caaatcgtcg
     4261 ggcgaaagcg cggacacggg gcggatgtct ttgtcgagga atccgtcgtg ttgtacgaat
     4321 acgccgagtt cttcgtcatg aggacggtac atattcgcgc tgatgtccgc ccatttttcc
     4381 aactcggcgg cgctcacgtt caaatccgga cgcgggtatt tcgccagggc ttcgcgggtg
     4441 tagtccaata cccatgcggc gagggtgttg gtgtaccagt tgttgttgat gttgttttcg
     4501 tattcgttcg ggccggttac gccgtgaatc atgtatttgc cgctgcgttt ggagaagtgg
     4561 acgcggtccg cccagaagcg ggacacttcg accaagactt ccaagccttc tttggcaaga
     4621 tagctttcgt cgccggtgta gttggtgtag ttgtagatgg cgtaaggaat cgcgccattg
     4681 cggtggattt cctcgaaggt gatttcccat tcgttgtggc actcgatgcc ggtaaacgtt
     4741 accatcggat agagtgcgcc cgccaagccc tgttcgcgcg cattgtgctg cgcctgcggc
     4801 agttggttgc ggcggtattg cagcaggttg cgggtaactt cgggttccgc cagcgcgagg
     4861 tagagcggta cggcgtaggc ttcagtatcc caataggttg cgccgccgta tttttctccg
     4921 gtaaagcctt tcgggccgat gttcaggcgc gcgtcttcgc cgtagtaggt ggagaacagt
     4981 tggaacagat tgaaacggat gccttgctgc gcttcgtcgt tgccttcgat gaccacgtcg
     5041 gcgatttccc aacggtgcag ccagcctgct ttgtgcgcgt ccagcaaggt ttcaaacgca
     5101 acgcctgcga ctttttccga c
//
LOCUS       DAWHSK010000139         5112 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.139, whole genome shotgun sequence.
ACCESSION   DAWHSK010000139 DAWHSK010000000
VERSION     DAWHSK010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5112
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.139"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(338..760)
                     /locus_tag="WIC03_001452"
     CDS             complement(338..760)
                     /locus_tag="WIC03_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEZ3883931.1"
                     /translation="MQVTSKWIDGMCFVGTTEGGHSVVMEGSAAEGKAKRGPSPLEML
                     LLGVAGCSSIDVVMIAEKQRQKVTDCRATVTAKRADDAPRVFTEIHIHFKVFGHDLKE
                     SAIERAVQMSAEKYCSASIMLGKAAKITHSFEIAGADK"
     gene            complement(878..1549)
                     /locus_tag="WIC03_001453"
     CDS             complement(878..1549)
                     /locus_tag="WIC03_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEZ3883932.1"
                     /translation="MMKTFKNIFSAAILSAALPCAYAAGLPQSAVLHYSGSYGIPATM
                     TFERSGNAYKIVSTIKVPLYNIRFESGGTVVGNTLHPTYYRDIRRGKLYAEAKFADGS
                     VTYGKAGESKTEQSPKAMDLFTLAWQLAANDAKLPPGLKITNGKKLYSVGGLNKAGTG
                     KYSIGGVETEVVKYRVRRGDDTVMYFFAPSLNNIPAQIGYTDDGKTYTLKLKSVQING
                     QSAKP"
     gene            complement(1565..2191)
                     /locus_tag="WIC03_001454"
     CDS             complement(1565..2191)
                     /locus_tag="WIC03_001454"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEZ3883933.1"
                     /translation="MKNIVILISGRGSNMQAIVNAAIPNVRIAAVLSNSETAAGLQWA
                     AGQGIPTDSLNHKNFESRLAFDTAMMEKIDAYQPDLVVLAGFMRILTPEFCAHYEGRL
                     MNIHPSVLPSFTGLHTHERALEAGCRVAGCTIHFVTAELDCGPIVSQGIVPILDGDTA
                     DDVAARVLAVEHKLYPKAVADFAAGRLIIEGNRVRNSENADAARFLTA"
     gene            complement(2298..3116)
                     /locus_tag="WIC03_001455"
     CDS             complement(2298..3116)
                     /locus_tag="WIC03_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ3883934.1"
                     /translation="MNTIFKISALTLSAALALSACGKKEAAPASASEPAAASSAQGDT
                     SSIGSTMQQASYAMGVDIGRSLKQMKEQGAEIDLKVFTEAMQAVYDGKEIKMTEEQAQ
                     EVMMKFLQEQQAKAVEKHKADAKANKEKGEAFLKENAAKDGVKTTASGLQYKITKQGE
                     GKQPTKDDIVTVEYEGRLIDGTVFDSSKANGGPVTFPLSQVIPGWTEGVQLLKEGGEA
                     TFYIPSNLAYREQGAGDKIGPNATLVFDVKLVKIGAPENAPAKQPAQVDIKKVN"
     gene            complement(3375..3815)
                     /locus_tag="WIC03_001456"
     CDS             complement(3375..3815)
                     /locus_tag="WIC03_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HEZ3883935.1"
                     /translation="MPKATFYTHVAQVPIFTCRLIARAIRDGGRILVWSDSFGQLQEL
                     DKMLWQYEAESFIPHEIWEMEEAMPSETSVLLACGGNPPRIPEGMTVLNLSDGFWNTA
                     PVLPARVLEIVGNSLEDLADARERFTAYRRSGFAIEHHGMEGKA"
     gene            complement(3878..>5112)
                     /locus_tag="WIC03_001457"
     CDS             complement(3878..>5112)
                     /locus_tag="WIC03_001457"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEZ3883936.1"
                     /translation="AVAVCCLKSTGRAALNKAAAEAAKWAQNQETVNVDIHAFDEAQA
                     AAVAEAFAIAFGNAAYRFDRYKKEAKPAKFETAVFHSAHEAVVKEALRVAEAQVYGQS
                     LCRDLGNAAPNECTPEFLARTAKAEAEKLGAHAKIIEKDYIKENMGSFWSVAKGSAEA
                     PYLVELSYFGAADKEAAPVVLVGKGITFDTGGISLKPGLNMDEMKFDMCGAATVISTF
                     CAAVKLQLPINLIAIVATCENMPSGAANKPGDVVKSMKGLTIEVLNTDAEGRLILCDA
                     LTYAEQFKPKAVIDVATLTGACIVALGHDVSGVMGNNQDLVDSLLAASYNVDDKAWQL
                     PLFETYKDQLKSNFADIPNIGTPGAGTITAATFLSYFTEDYPWAHLDIAGTAWKSGAE
                     KGATGRPVPLLLNYLRNL"
BASE COUNT         1082 a         1319 c         1403 g         1308 t
ORIGIN      
        1 gtactggttt ttgttaatcc actataattc aataaattaa tatatggctt aaaataacgg
       61 gatgcccgcc tcccgtcctc ctgcagaagc gggcgggtat cattttaaaa cgcggcattt
      121 aaaatttgac cgaaaattgt tgacaatccg gaatcaagtc tgcacaatac cccgacaagt
      181 ccaagtatta taaaggctga ataaagagga aacagcaggc agatatattc gggaggtgca
      241 gtccgaatat atctgctttt ttatgcgcct ccggattgcc tgccgcacct ttcccttcag
      301 acggcatcag ccgtttcccc ataatgccgc ccgatgccta tttatctgcc ccggcaattt
      361 caaaactgtg ggtaatcttt gccgctttgc ccaacataat cgaagccgaa cagtattttt
      421 cggcagacat ctgaacggcg cgctcaatgg ccgattcttt caaatcatgc ccgaatactt
      481 tgaaatggat gtggatttcg gtaaacacgc gcggcgcatc gtccgcccgt ttcgccgtaa
      541 ccgtcgcacg gcagtcagtc actttctgac gctgtttttc ggcaatcatc accacatcga
      601 tgctcgaaca gcccgccacg cccaacagca gcatttccaa agggctgggc ccgcgcttag
      661 ccttaccttc tgccgccgac ccctccataa cgacgctgtg cccgccttcc gttgttccga
      721 caaagcacat tccgtctatc cattttgatg taacctgcat ggtgtcattc ctgaaaatag
      781 cgttaaaacc gctttgcata tggcgttatt gtaaacaatt tcaagcggct tatgcagaaa
      841 tatggacaaa acggcaaaaa aacgcctgaa aatcgattta cggtttggcg gactggccat
      901 tgatctgcac cgatttgagt ttcagcgtat aggttttgcc gtcgtcggta tagccgattt
      961 gtgccggaat attgttcagg gacggtgcga agaaatacat taccgtatcg tcgccgcgcc
     1021 gcacccgata tttgacgact tcggtttcca cgccgcctat gctgtatttt cctgtacccg
     1081 ccttattcaa accgccgacg gaataaagtt ttttgccgtt ggtgattttc agccccgggg
     1141 ggagtttcgc gtcatttgcc gccaactgcc aggcaagcgt gaacaaatcc atagccttgg
     1201 ggctttgctc ggttttgctc tcgcccgctt tgccgtaggt tacgctgccg tcggcgaatt
     1261 tggcttccgc atacagtttg cccctgcgta tgtctctata gtaggtaggg tgcagggtat
     1321 tgccgacaac cgtaccgccg gactcgaaac ggatattgta tagcggcact ttaatcgtcg
     1381 aaacgatttt gtaagcattg ccgctgcgtt caaatgtcat cgtggcggga atgccgtagc
     1441 tgccggaata gtgcagcacg gcggattggg gcagccctgc cgcatacgcg cacggcaggg
     1501 cggcggacaa aatggcggcg gaaaatatat ttttaaaagt cttcatcatt tgctcccgcc
     1561 cggtttacgc cgtcaggaaa cgggcggcat cggcgttttc cgaatttctg acgcggtttc
     1621 cctcgataat caggcggccg gcggcaaaat cggcaacggc tttcggatag agtttgtgtt
     1681 cgacagccaa aacccgggcg gcaacatcgt ctgccgtatc gccgtcgagt atcggcacaa
     1741 tcccttgcga tacaatcggg ccgcaatcca gttcggcagt aacgaaatgg atggtgcagc
     1801 cggcaacgcg gcagcccgcc tccaaagcgc gttcgtgcgt atgaagtccg gtaaacgagg
     1861 gcaggacgga cgggtgaatg ttcatcagcc tgccttcgta atgggcgcaa aactcagggg
     1921 tcagaatccg cataaaacct gccaaaacca ccaagtcggg ttgatatgcg tcgatttttt
     1981 ccatcatggc ggtatcgaag gcaagtcgtg attcaaagtt tttatgattc aggctgtcgg
     2041 tcggaatacc ctgaccggcc gcccattgca aaccggcagc cgtttcgctg ttgctcaaca
     2101 cggcggcaat acggacgttg ggaatggcgg cattgacgat tgcctgcata ttgctgccgc
     2161 gtccggaaat caggatgacg atgtttttca taatggtgcg cttttgaaag ggatgccatc
     2221 tgaaccgctg tttggtggtt tcagacggca tttgccgtaa aaatgcccga aaacctgttt
     2281 cgggcatgga ttcggactta atttactttt ttgatgtcga cttgagccgg ctgcttggcg
     2341 ggcgcgtttt cgggtgcgcc gattttgacc agtttcacat caaataccaa agtggcgttc
     2401 ggaccgattt tgtcgcccgc accctgttcg cggtaggcaa ggttggacgg gatgtagaac
     2461 gtggcttcgc cgccttcttt cagaagctgt acgccttcgg tccaacccgg aatcacttgg
     2521 ctcaaaggga aggtgaccgg gccgccgttg gctttgctgc tgtcgaatac cgtaccgtca
     2581 atcaggcggc cttcgtattc cacggtaacg atgtcgtctt tggtcggctg tttgccttca
     2641 ccctgtttgg tgattttgta ttgcaggccg gaagcagtgg tcttcacgcc gtctttggcg
     2701 gcattttctt tcagaaaggc ttcgcctttt tctttattgg ccttcgcgtc cgccttgtgt
     2761 ttttctacgg ctttagcctg ttgttcctga aggaatttca tcatgacttc ctgagcctgc
     2821 tcttcggtca ttttgatttc tttgccgtca tacactgcct gcatggcttc ggtaaagact
     2881 ttcaaatcga tttccgcgcc ctgttccttc atttgcttca gggagcgtcc gatgtccacg
     2941 cccatcgcat agcttgcctg ctgcatcgtg ctgccgatcg aagaggtgtc gccctgcgcg
     3001 gaagaagcgg cggcaggttc ggatgcagat gcgggggcgg cttctttttt gccgcaggcg
     3061 gaaagtgcca aagcggcgga aagggtcagt gcgctgattt tgaaaatggt gttcatgatg
     3121 gatcttcgct gtcgataagg tcggaaaaac gggattatag ccgagtttga atgtttcaac
     3181 acacaggatg acacataaag cgtcaatcgt gtgttgccct gttttggaaa ggattgaacc
     3241 ttccaaaata agttttgatt ctaccgcccc gagggacaga tgtccaagtg gcggggttca
     3301 accgataagg aaattttaat caaatagaat caagcctgtt taaattttgt aaatgcggca
     3361 tttcagacgg cattttatgc cttgccctcc atgccgtgat gttcgatggc aaaaccgctt
     3421 cggcggtagg cggtaaagcg ttcgcgcgcg tcggcgagat cttccaagct gttgccgacg
     3481 atttccaaaa cgcgcgcggg caagaccgga gcggtgttcc aaaaaccgtc ggacaggttc
     3541 aaaacagtca tgccttcggg aattcggggc ggattgccgc cgcaagcaag caggacggat
     3601 gtttcagacg gcatggcttc ttccatttcc caaatttcgt gcggaataaa actctcggcc
     3661 tcgtattgcc aaagcatttt gtccaattcc tgaagctgcc cgaacgaatc ggaccacacc
     3721 agtatcctgc cgccgtcccg aatcgcacgg gcaatcaggc ggcaggtgaa aatcggaact
     3781 tgggcaacgt gcgtgtaaaa ggtggctttc ggcatattgt ttgagcattt ggcaggacga
     3841 tgccgtctga aaggcttcag acggcattgt ggcaaaatta aagattccgc agatagttca
     3901 gcagcaaggg aacgggacgg ccggtcgcac ctttttccgc accggatttc cacgccgtac
     3961 ccgcgatgtc aaggtgtgcc catggatagt cttcggtaaa gtaggatagg aatgttgcgg
     4021 cggtaatcgt gcccgcgccg ggcgtgccga tgtttggaat gtcggcaaag ttggatttga
     4081 gttggtcttt gtaggtctca aagagcggca gttgccacgc tttgtcgtcc acgttgtagg
     4141 aagcggcaag caggctgtcg accaaatctt gattgttgcc catcacgccg ctgacatcgt
     4201 gccccaaggc aacaatacac gcaccggtca gggtggcgac gtcgatgacg gctttgggtt
     4261 tgaactgttc ggcgtaagtg agcgcgtcgc acaaaatcag acggccttca gcatcggtgt
     4321 tcaacacttc gatggtcagg cctttcatgc tttttacgac gtcgcccggt ttgttggccg
     4381 cgccggaagg catattttca caagtggcga cgatggcaat caggttgatc ggcagttgca
     4441 gtttgacggc ggcgcagaag gtgctgatga cggttgccgc gccgcacata tcaaacttca
     4501 tttcgtccat gttcagaccg ggtttgaggg aaatgccgcc ggtgtcgaag gtaatgcctt
     4561 tgccgaccaa gacgacgggg gcggcttctt tgtcggctgc accgaaatag ctcagttcga
     4621 ccaaataggg ggcttccgcg ctgcctttgg cgaccgacca aaacgaaccc atgttttctt
     4681 tgatgtagtc tttttcgatg attttggcgt gcgcgcccag tttttcggct tcggctttgg
     4741 cggtgcgcgc taaaaattcg ggcgtgcatt cgttgggcgc ggcgttgccc aagtcgcggc
     4801 agaggctttg tccgtaaact tgcgcttcgg cgacgcgcaa ggcttcttta acgacggctt
     4861 cgtgtgcgct gtggaacacg gcggtttcaa atttggcggg cttggcttct tttttgtagc
     4921 ggtcgaaacg gtaggcggcg ttgccgaacg caatcgcaaa cgcttcggct acggcggcgg
     4981 cttgcgcttc atcgaaggca tggatgtcca cattgaccgt ttcctgattt tgcgcccatt
     5041 tggcggcttc ggcggcggcc ttgttcaatg cggcgcggcc ggtgcttttc agacagcata
     5101 cggcaacagc ct
//
LOCUS       DAWHSK010000140         5077 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.140, whole genome shotgun sequence.
ACCESSION   DAWHSK010000140 DAWHSK010000000
VERSION     DAWHSK010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5077
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.140"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            272..1504
                     /gene="pilG"
                     /locus_tag="WIC03_001458"
     CDS             272..1504
                     /gene="pilG"
                     /locus_tag="WIC03_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus assembly protein PilG"
                     /protein_id="HEZ3883937.1"
                     /translation="MAKNGGFSLFAKKEKRFIFEGRHSASDKLVNGEVSAFTEEEARK
                     KLAKRGIRPLQITRVKTSSKRKITQEDITVFTRQLSTMIKAGLPLMQAFEIVARGHGN
                     PSMTEMLMEIRGEVEQGSSLSRAFSNHPKYFDRFYCNLVAAGETGGVLESLLDKLAIY
                     KEKTQAIRKKVKTALTYPVSVIAVAIGLVFVMMIFVLPAFKEVYANMGAELPALTQTV
                     MDMSDFFVSYGWMVLIALGFAIYGFLKLKARSIKIQRRMDAILLRMPIFGDIVRKGTI
                     ARWGRTTATLIAAGVPLVDVLDSTAGAAGNLIYEEATREIRTRVIQGLSMTSGMRATE
                     LFPNMMLQMSSIGEESGSLDDMLNKAAEFYEDEVDNAVGRLSAMMEPIIIVILGLVIG
                     TLLVAMYLPLFNLGNVVA"
     gene            1578..2438
                     /locus_tag="WIC03_001459"
     CDS             1578..2438
                     /locus_tag="WIC03_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEZ3883938.1"
                     /translation="MSDLSVLSPFAVPLAAVFGLLVGSFLNVVIYRVPVMMERGWTVF
                     AKEYLNLPLTEEESRTFNLMKPDSCCPKCRVPIRAWQNIPIVSYLLLRGKCASCQTKI
                     SIRYPLIELLTGVLFGLVAWQYGWSWITLGGLVLTAFLISLTFIDADTQYLPDSMTLP
                     LIWLGLIFNLDGGFVPLQSAVLGAVAGYGSLWLLCAVYKLLTGKTGMGNGDFKLIAAL
                     GAWLGISALPVLIFVSSLIGLVAAIVMRVAKGQHFAFGPALTVSGWIIFTANDSVWRA
                     VNWWLTHPVL"
     gene            2440..3072
                     /locus_tag="WIC03_001460"
     CDS             2440..3072
                     /locus_tag="WIC03_001460"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEZ3883939.1"
                     /translation="MTVWVGLTGGIGSGKSAAAQCFADLGVPRIDADATAHSLTASDG
                     IALPEIRRLFGDTVFDTQGLLRRDILRKEIFASPSRKALLESVMLPLIFSEIKKQQET
                     FADAAYGIVEIPLLTEKRQFISLIRRVLTISAPLEKRIGRVMARSGLTRGEVAAVISH
                     QASESERLLLADDVLLNDGSLKSLREKTMRLHAFYSGIFASKPTQGKHNG"
     gene            3065..3274
                     /locus_tag="WIC03_001461"
     CDS             3065..3274
                     /locus_tag="WIC03_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HEZ3883940.1"
                     /translation="MAESRQTRLQVKCPTCQTAVVWEPENAFRPFCSQRCKLIDLGGW
                     ADGKYTVSGQTESLPEISEPDMAYR"
     gene            complement(3359..5035)
                     /gene="pilB"
                     /locus_tag="WIC03_001462"
     CDS             complement(3359..5035)
                     /gene="pilB"
                     /locus_tag="WIC03_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HEZ3883941.1"
                     /translation="MSVGLLRILVQNQVVTVEQAEHYYKESQAGKEVLPMLFSDGVIS
                     PKSLAALIARVFSYSILDLRHYPRHRVLMGVLTEEQMVEFHCVPVFRRGDKVFFAVSD
                     PTQMPQIQKTVSAAGIEVELVIVEDDQLAGLLDWVGSRSTSLLQELGEGQEEEESHTL
                     YIDNEEAEDGPVPRFIHKTLSDALRSGASDIHFEFYEHNARIRFRVDGQLREVVQPPI
                     AVRGQLASRIKVMSRLDISEKRIPQDGRMQLTFQKGGKPVDFRVSTLPTLFGEKVVMR
                     ILNSDAASLNIDQLGFEPFQKKLLLEAIHRPYGMVLVTGPTGSGKTVSLYTCLNILNT
                     ESVNIATAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETA
                     DIAIKAAQTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSS
                     CKQEVERPSASALKEVGFTDEDLAKDWKLYRAVGCDRCRGQGYKGRAGVYEVMPISEE
                     MQRVIMNNGTEVDILDVAYKEGMVDLRRAGILKVMQGITSLEEVTANTND"
BASE COUNT         1203 a         1420 c         1271 g         1183 t
ORIGIN      
        1 ttgagctaag gcgagccaac gctgtaccgg tttttgttaa ttcactatac cctccggccg
       61 gcatttgacc cgcaacaata tcctgcaaga tttattgtgt acgcataaat gccggacagc
      121 cgcctaaata aggtataatc gcatccgatt ctgtcccgtt tgccgcccgc aggcgggatg
      181 tcggcggttt tcatcttacc gggaacacgc cggtactgtt ttttaacgat gtttcttaca
      241 ttttattcca attactttac ggggctagaa tatggctaaa aacggaggat tttctttgtt
      301 cgcaaagaaa gaaaaacgct ttatctttga aggcaggcat tccgcctccg acaaactggt
      361 caacggcgaa gtatccgcgt ttaccgaaga agaggcgcgc aaaaaactgg caaaacgcgg
      421 catccgcccg ttgcagatta cccgtgtgaa aacaagctcc aagcgcaaaa tcacacaaga
      481 agacatcacc gttttcaccc gccagctgtc cacgatgatt aaagcgggcc tgccgctgat
      541 gcaggcattt gaaatcgtgg cgcgcggaca cggcaacccg tctatgacgg aaatgctgat
      601 ggaaatccga ggcgaagtgg aacagggcag ctcgttgagc cgcgcattct caaaccaccc
      661 gaaatatttc gaccgcttct actgcaacct ggttgcggca ggcgaaacgg gcggcgtatt
      721 ggaaagcctg ctggacaaat tggcaattta caaagaaaaa acccaggcta tccgcaaaaa
      781 ggtaaaaacc gcactgacct atccggtatc cgtcatcgcc gtcgccatcg gtttggtatt
      841 cgtgatgatg attttcgtac tgcccgcctt taaagaagtt tacgccaata tgggcgcgga
      901 gcttcccgca ctgacccaaa cagtgatgga tatgtccgac tttttcgtct catacggctg
      961 gatggtgctg atcgcactgg gctttgccat atacggcttc cttaaattga aggcgcgttc
     1021 gattaaaatc caacggcgta tggatgccat actgctgcgt atgccgattt tcggagacat
     1081 tgtccgcaaa gggacgattg cccgctgggg caggacgacg gcgacgctga ttgcggcagg
     1141 cgtgcctttg gtcgatgtat tggactccac tgccggcgcg gcgggcaatt taatctatga
     1201 agaagccacc cgggaaatcc gtacgcgcgt catccaaggt ttgtctatga cttcggggat
     1261 gcgtgcgacg gaactgttcc ccaatatgat gttgcagatg tcttccatcg gcgaggaatc
     1321 gggttctttg gacgatatgc tcaacaaagc cgccgaattt tacgaagacg aggtggacaa
     1381 tgcggtcggc aggctgtccg ctatgatgga gccgatcatt atcgtgattt taggcttggt
     1441 catcggcacg cttctggtcg ccatgtatct gccgctgttt aatttgggca acgtggtcgc
     1501 ctgatttgcc gcacagatcc ggcgcggatt cgctctgcgc cggtttgttt ttgttttgaa
     1561 tacattgaga acaaaatatg tctgatttgt ctgtattgtc gccgtttgcc gtgcctttgg
     1621 cagcggtgtt cgggctgctg gtcggcagtt tcttaaacgt cgtcatttac cgcgtgccgg
     1681 tcatgatgga acgcggctgg acggtatttg ccaaagaata tttaaacctg ccgctgaccg
     1741 aagaggaaag ccgtaccttc aacctgatga agccggattc ctgctgtccc aaatgtcgcg
     1801 tgccgatacg cgcgtggcag aacatcccga ttgtcagcta cctgctcctg cgcggcaaat
     1861 gcgcttcctg ccaaaccaaa atcagcatac gttatccctt aatcgagctg ctgaccggcg
     1921 tattgttcgg gctggtcgcc tggcaatacg gctggtcttg gattacgctg ggcggattgg
     1981 tactgaccgc gtttctgatt tccctgacct ttatcgatgc ggacacccaa tacctgcccg
     2041 actcgatgac actgccctta atttggctgg gtctgatatt taatttggac ggcggcttcg
     2101 tgcctttgca gtctgccgtt ttaggtgcgg tcgccggcta tggttcatta tggctcttat
     2161 gtgcagtgta taaactgctc acaggaaaaa ccggtatggg caacggagat ttcaaactga
     2221 ttgccgcatt gggcgcgtgg ctcggcatat ccgcattgcc cgtactgatt tttgtttcct
     2281 cgctgatcgg tttggtcgcg gcaatcgtta tgcgcgtcgc caaggggcag cattttgcct
     2341 tcggccccgc actgacagtt tcgggctgga taatttttac ggcaaacgat tccgtatggc
     2401 gggcggtcaa ctggtggctg acccatccgg tgctgtaaga tgacggtatg ggtcgggctg
     2461 accggcggaa tcggcagcgg caaatcggca gccgcgcaat gttttgccga tttgggcgtg
     2521 ccgcgcatcg atgcggacgc gacggcgcac tcgctgacgg cttcagacgg catcgccctg
     2581 ccggaaatca ggcggctgtt cggcgacacc gttttcgaca cacagggttt gttgcggcgc
     2641 gacatattgc gtaaagaaat ctttgcctcc ccatcgcgaa aagccttgct cgaatccgtg
     2701 atgttgccgc tgattttctc agaaatcaaa aaacagcaag aaacctttgc cgatgcggct
     2761 tacggcattg tcgaaatccc gctgctgacg gaaaagcgtc aatttatcag cctgatacgg
     2821 cgtgtcctga ccataagcgc acctttggaa aaacgtatcg gcagggtgat ggcccgcagc
     2881 gggctgacgc gcggcgaggt ggctgccgtc atcagccatc aggcatccga atccgaacgc
     2941 ctgctgcttg cagacgatgt gctgctcaat gacggcagcc tcaaaagcct gcgtgagaaa
     3001 acaatgcgcc tgcacgcgtt ttattcaggg attttcgcct caaaaccaac acaaggaaaa
     3061 cacaatggct gaatcgcggc aaacacgcct tcaagtcaaa tgtccgacct gtcaaacagc
     3121 agtagtatgg gaacccgaaa acgcattccg ccccttctgt tcgcaacgct gcaaactgat
     3181 cgacttgggc ggatgggcag acgggaaata tacggtttcc ggccaaacgg aaagtttgcc
     3241 ggaaatatcc gaacccgaca tggcataccg ctgaccgccc cgccttcccg gcaaacaccc
     3301 tgaaagtcaa atgccgtctg aaacaaacac gcttcagacg gcattttcat tctcaaacct
     3361 aatcgttggt atttgccgtt acctcttcca atgaagtaat gccctgcata actttcaaaa
     3421 taccggcccg gcgcaaatcc accataccct ccttataggc aacgtccaaa atatccactt
     3481 ccgtaccgtt gttcataatc acacgctgca tttcttcgct gatgggcata acctcataca
     3541 cgcccgcacg ccccttataa ccctgccccc ggcaacggtc gcaaccgacg gcgcggtaaa
     3601 gtttccaatc ttttgcaagg tcctcatcgg tgaagccgac ttccttcaaa gcagaggcag
     3661 acgggcgttc cacttcctgt ttgcagctcg aacacagcct gcgtaaaaga cgctgcgcca
     3721 taatcaggct gaccgaactg gcaatattaa acggcgcgac acccatattc agcatacgcg
     3781 acaacgtcgc cggcgcatta ttggtgtgca gggtggaaaa caccatatgc cctgtttgtg
     3841 ccgccttaat cgcaatatcg gcagtttcca aatcacgaat ctcaccgacc ataatgatgt
     3901 ccgggtcctg acgcaggaaa gacttcaaag cagcggcaaa agtcaggccc tgcttatcat
     3961 tgacgttaac ctgattgatg cccggcaggt taatctcggc agggtcttcc gccgttgcaa
     4021 tatttaccga ctccgtattc aaaatattca aacaggtata gagcgacacc gtcttacccg
     4081 aacccgtcgg accggttacc agcaccatcc cgtagggacg gtgaatcgct tccaacaaca
     4141 attttttctg aaacggctca aaaccgagct ggtcgatgtt caaagacgcg gcatcggaat
     4201 tcaaaatccg catcacgacc ttttcgccaa acagcgtcgg caatgtgctg acacggaaat
     4261 cgacaggctt gccgcccttt tgaaaggtca gctgcatcct gccgtcctgc ggtatccgtt
     4321 tttcggaaat gtccaaacgc gacattacct taatccggga agcaagctgc ccccttaccg
     4381 caatgggcgg ctgaaccacc tcgcggagct gcccgtccac acggaaacgg atacgggcat
     4441 tgtgttcgta aaactcgaaa tggatgtcgg atgccccgct gcgcaaggca tccgacaaag
     4501 tcttatggat aaacctcgga acagggccgt cttctgcctc ctcgttgtcg atatacaggg
     4561 tgtggctttc ctcttcctcc tgcccctccc caagctcctg aagcagcgat gtcgaacgcg
     4621 aacccaccca atcgagcaaa cccgccaact ggtcatcctc gacaatgacc aattcaacct
     4681 caatccctgc ggcagaaacg gttttctgaa tttgcggcat ctgtgtcgga tcggaaaccg
     4741 caaaaaatac tttgtcgccc cgacggaaaa ccggcacaca gtggaactcc accatctgct
     4801 cctccgtcaa cacccccatc agcaccctgt ggcgcggata atgacgcaaa tcaagaatcg
     4861 aataactgaa cacccttgca atcaatgccg caagcgactt gggcgaaatg acgccgtctg
     4921 aaaacagcat cggcaacact tccttacccg cctgcgactc cttgtagtaa tgctcggcct
     4981 gctcaacagt aaccacctgg ttttgaacca gaatcctcag caaacctacg ctcatacgac
     5041 cttatcccca aatttattca ttgttatacc tgtacag
//
LOCUS       DAWHSK010000141         5071 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.141, whole genome shotgun sequence.
ACCESSION   DAWHSK010000141 DAWHSK010000000
VERSION     DAWHSK010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5071
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.141"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..201)
                     /gene="cca"
                     /locus_tag="WIC03_001463"
     CDS             complement(<1..201)
                     /gene="cca"
                     /locus_tag="WIC03_001463"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221678.1"
                     /note="catalyzes the addition and repair of the essential
                     3'-terminal CCA sequence in tRNAs without using a nucleic
                     acid template; phosphohydrolase activities include
                     hydrolysis of pyrophosphate, 5'-nucleoside tri- and
                     diphosphates, NADP, and 2'-AMP with the production of Pi,
                     metal-dependent phosphodiesterase activity for 2',3'-cAMP,
                     2',3'-cGMP, and 2',3'-cCMP, and hydrolysis 2',3'-cyclic
                     substrates with the formation of 2'-nucleotides and
                     3'-nucleotides; these phosphohydrolase activities are
                     probably involved in the repair of the tRNA 3'-CCA
                     terminus degraded by intracellular RNases; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA tRNA nucleotidyl
                     transferase/2'3'-cyclic
                     phosphodiesterase/2'nucleotidase/phosphatase"
                     /protein_id="HEZ3883942.1"
                     /translation="MQTYLVGGAVRDYLLGLPVKDRDWVVVGADAQTMLAQGFQPVGK
                     DFPVFLHPETHEEYALARTERKT"
     gene            complement(286..1914)
                     /locus_tag="WIC03_001464"
     CDS             complement(286..1914)
                     /locus_tag="WIC03_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HEZ3883943.1"
                     /translation="MISTNGITMQFGAKPLFENVSVKFGEGSRYGLIGANGSGKSTFM
                     KILGGDLEQTAGEVAIENGVRLGKLRQDQFAYEDMRVLDVVMMGHTEMWAAMTERDAI
                     YANPEATEDDYMKAAELEAKFAEYDGYTAEARAAELLSGVGISEDLHNAKMAEVAPGF
                     KLRVLLAQALFSKPDVLLLDEPTNNLDINTIRWLEGVLNQYDSTMIIISHDRHFLNEV
                     CTHMADLDYNTITIYPGNYDDYMLASAQSRERALKDNAKAKEKLQELQEFVARFSANK
                     SKARQATSRLKQADKIKSEMVEVKPSTRQNPYIRFEADEKAKLHRQAVEVEKLAKRFE
                     TQLFKNLNFILEAGQRLAIIGPNGAGKSTLLKLLAGAYNPEYSDGLLPDEGSIKWAEK
                     ASVGYYPQDHENDFDVDMDLSEWMRQWGQEGDDEQVIRGTLGRLLFGSNDVVKKVKVL
                     SGGEKGRMLYGKLLLLKPNVLVMDEPTNHMDMESIESLNMALEKYNGTLIFVSHDRQF
                     VSSLATQIIELDGKGGYEHYLGDYESYLEKKGVA"
     gene            2064..2438
                     /locus_tag="WIC03_001465"
     CDS             2064..2438
                     /locus_tag="WIC03_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HEZ3883944.1"
                     /translation="MIVLHGIPNCDTVKKAKNRLAGYGLEFEFRDFKKQMPSEAEICS
                     WLEQVPLETLLNKRGTSWRKLDAETQQKALSSTAEAVKLMSEMPSLIKRPVLECGGKV
                     YAGFSEETYDGIFNRQAPCRQG"
     gene            2492..4030
                     /locus_tag="WIC03_001466"
     CDS             2492..4030
                     /locus_tag="WIC03_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEZ3883945.1"
                     /translation="MFHSIEKYRTPAQVLLGLIALTFVGFGVSTVSHPGADYIVQVGD
                     EKISDHSINNAIQNEQADGGGPSRDAVFQSLLQRAYLKQGAKLLGISVSSEQIKQIIV
                     DDPNFHDANGKFDHALLNRYLSQRHMSEDQFVEEIRDQFALQNLVNLVQNGVLVGDAQ
                     AEQLIRLTQVNRTIRSHTFNPDEFIAQVKVSEADLQKFYNANKKDYLLPQAVKLEYVA
                     LNLKDFADKQTVSETEVKNAFEERVARLPANEAKLSFEQEKAAVENELKMKKAVADFN
                     KAKEKLSDDAFNHPASLAEAAKNSSLKVETQETWLSRQDAQMSGLPENLINAIFSDDV
                     LKKKHNSEVLSINNEIAWVVRAKEVREEKTLPFAEAKDAVRQAYIRAEAAKLAENKAK
                     DVLTQLNGGKAVDVKWSEVSVLGAQQARQSMPPEAYAELLKAKPTNGKPAYVRLTGLP
                     APVIVEVQAITPPDDIAAQLPLAKQALAQQQSANTFDLLIRYFNGKIKQTKGAQSVDN
                     GGGQ"
     gene            4097..4714
                     /gene="recR"
                     /locus_tag="WIC03_001467"
     CDS             4097..4714
                     /gene="recR"
                     /locus_tag="WIC03_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEZ3883946.1"
                     /translation="MSHKKQDAFQGLIDALKVLPNVGPKSAQRIAYHLLQHKRKEAEK
                     LVDALQTALKQVHHCAMCNTFCEGGLCDICADETRDGRRLMVVHMPADVSNMEVANCH
                     DGLYFVLMGQINTALGMDVSAIALDRLAQRLGGGEVEEIIIATAFTAEGNATAYVLSE
                     FFKNLPYKVSRLSQGIPLGGELEYVDAGTLAQAVYERRLIKEGGA"
     gene            complement(4770..5048)
                     /locus_tag="WIC03_001468"
     CDS             complement(4770..5048)
                     /locus_tag="WIC03_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883947.1"
                     /translation="MNIRTAFALCAIALSAAAAAYAKEIKIDANNTPYSEADAQKLAA
                     TAVGMGVKEPISLNGGSGSITVSGSSATQCVFKVGNGGALQIQGLNCK"
BASE COUNT         1229 a         1309 c         1350 g         1183 t
ORIGIN      
        1 ggttttgcgc tcggtgcggg cgagggcgta ttcttcgtgt gtttcgggat ggagaaacac
       61 gggaaagtct ttgccgactg gctggaagcc ttgcgccagc atggtttgtg cgtctgcgcc
      121 gacgaccacc caatcgcggt ctttgacggg caagcccaaa agataatcgc ggacggcgcc
      181 gccgacgagg taagtctgca tatgcttcct attttaaagt tatcaacaat gccgtctgaa
      241 gcggcttcag acggcattgt ttcaaccgga gggtattttg tgcgcttatg ctacgccttt
      301 tttctccaag taactttcgt aatcgcccaa gtagtgttca tatccgcctt taccgtccaa
      361 ttcgatgatt tgggtagcca atgaagatac aaactgacgg tcatgcgaga cgaaaatcag
      421 cgtgccgttg tatttttcca gcgccatgtt caaggattcg atgctttcca tgtccatgtg
      481 gttggtcggt tcatccatga ctaagacatt gggtttcaac agcaacagtt tgccgtaaag
      541 catacggcct ttttcaccac cggagagaac cttcactttt ttcacgacat cgttactgcc
      601 gaagagcaaa cgccccaaag tgccgcggat gacttgttcg tcgtcgcctt cctgccccca
      661 ttggcgcatc cattcgctca aatccatatc gacgtcgaag tcgttttcat ggtcttgcgg
      721 atagtagccg acactggctt tttccgccca tttgatgctg ccttcgtccg gtaacaggcc
      781 gtctgaatat tcggggttgt acgcgccggc caagagtttc agcagggtgg atttgcccgc
      841 gccgttcggg ccgatgatgg cgaggcgttg tcccgcttca aggatgaagt tcaggttttt
      901 aaacaactgg gtttcaaagc gtttcgccag tttttcaact tccacagcct gacggtgcag
      961 cttggctttt tcatcggctt caaaacggat atacgggttt tgacgggtgg aaggtttgac
     1021 ttcgaccatc tccgatttga ttttgtcggc ctgtttcaga cggctggttg cctgacgggc
     1081 tttggatttg ttggcagaga agcgtgcgac gaactcttgc agctcttgca gtttctcttt
     1141 ggctttggcg ttgtctttca gggcgcgctc acgcgattgg gcggaagcga gcatgtagtc
     1201 gtcgtagttg ccaggataga tggtgatggt gttgtagtcc aaatccgcca tgtgggtgca
     1261 gacttcgttc aaaaagtggc ggtcgtgcga gatgatgatc atggtggagt cgtattggtt
     1321 caacacgcct tccagccagc ggatggtgtt gatgtccaag ttattggtcg gttcgtcaag
     1381 caacagcaca tccggcttgg agaacagggc ttgcgccagc aatacgcgca gtttgaagcc
     1441 cggggcgact tccgccattt tcgcattgtg caaatcttcg gaaatgccca cgccgctcaa
     1501 cagttcggcg gcacgcgctt cggcggtgta gccgtcgtat tcggcgaact tggcttccag
     1561 ttcggcggct ttcatgtagt cgtcttcggt ggcttcggga ttggcgtaaa tcgcatcacg
     1621 ttcggtcatc gccgcccaca tttcggtatg ccccatcatc accacgtcca gcacgcgcat
     1681 gtcttcgtag gcaaactggt cttggcgcaa tttacccaaa cgcacgccgt tttcaattgc
     1741 gacttcaccg gcggtctgtt ccaaatcgcc gccgaggatt ttcatgaagg tggatttgcc
     1801 tgagccgttg gcgccgatca aaccgtagcg gctgccttcg ccgaatttga cggatacgtt
     1861 ttcaaatagc ggcttcgcgc cgaactgcat ggtgatgccg ttggtagaaa tcatgattgg
     1921 taaagccttt attgaacaag agattaaatt tttcgaattt tcggattgta acataaccgc
     1981 cccgccgtct gaaacagacg ggcaaatttt cagacactta tagtaaaata tccgttttat
     2041 tatttcagac aaaaggtttc caaatgatag tccttcacgg catcccaaat tgcgatacgg
     2101 tcaaaaaagc caaaaaccga cttgccggat acggcttgga gtttgagttt cgggatttca
     2161 aaaaacagat gccgtctgaa gcggaaatct gctcgtggct ggaacaagtg cctttggaaa
     2221 ccctgctcaa caaacgcggg acaagctggc gcaaactcga tgccgaaaca cagcaaaaag
     2281 ccctgtcctc gacagcggag gccgtcaaac tgatgtccga aatgccgagc ctgatcaagc
     2341 gtcccgtatt ggagtgcggc ggcaaggttt acgccggctt cagcgaagaa acctacgacg
     2401 gcatcttcaa ccggcaagcc ccgtgcagac agggataaaa accgttacaa tacccgactt
     2461 gaatttccca ttctatatcc cgatttaaaa tatgttccat tccatcgaaa aatacagaac
     2521 gcccgcccaa gtccttttgg gcctgattgc attaaccttc gtcggcttcg gggtcagcac
     2581 ggtatcccat ccgggtgccg actacatcgt ccaagtgggc gacgaaaaaa tcagcgacca
     2641 ctccatcaac aacgccatac agaacgaaca ggcggacggc ggcggccctt cgcgcgacgc
     2701 ggtgttccaa tccctgctgc aacgcgccta cctgaaacag ggcgcgaagc tgttaggcat
     2761 ttcggtttct tccgaacaaa tcaagcaaat tatcgtggac gatcccaatt tccacgacgc
     2821 aaacggcaaa ttcgaccacg cgcttttaaa ccgctacctt tcccaacgcc atatgtctga
     2881 agaccagttt gtcgaagaaa tccgcgatca gtttgccttg cagaatttgg taaacctcgt
     2941 ccaaaacggc gtattggtcg gcgacgcgca ggcggaacag ctgatcaggc tgacgcaggt
     3001 caaccgcacc atccgttcgc acactttcaa ccccgacgag ttcatcgccc aagtcaaagt
     3061 gtctgaagcc gatttgcaga aattttataa tgcgaacaaa aaagactatc tgctgccgca
     3121 ggcggtcaaa ttggaatatg tcgccttgaa tctgaaggat tttgcagaca agcaaaccgt
     3181 cagtgaaacg gaagtgaaaa atgcgtttga agagcgcgtg gcgcgtttgc cggcaaatga
     3241 agccaaactt tctttcgagc aggaaaaagc cgccgtcgaa aacgaattga aaatgaaaaa
     3301 ggcggttgcc gacttcaata aggcaaaaga aaagctgagc gatgacgcgt tcaaccatcc
     3361 cgcttcgctt gccgaagccg ccaaaaacag cagtttgaaa gtcgaaaccc aagaaacttg
     3421 gctgagcagg caggacgcgc aaatgtccgg actgcctgaa aacctgatca atgccatatt
     3481 cagcgacgac gtattgaaga aaaaacacaa ttccgaagtg ttgtctatca ataatgaaat
     3541 cgcgtgggtc gtccgcgcca aagaagtccg cgaagagaaa accctgccgt ttgccgaagc
     3601 caaagatgcg gtgcgtcagg cttatatccg tgccgaagcc gccaaacttg ccgaaaacaa
     3661 ggcaaaagac gtgcttaccc aactgaacgg cggcaaggca gttgatgtga aatggtcgga
     3721 agtgtccgtt ttgggcgcgc agcaggcaag gcagtccatg ccgcccgagg cttatgcgga
     3781 actgctgaaa gcaaaaccga caaacggcaa acccgcctac gtcaggctga ccggtctgcc
     3841 ggcacccgtg attgtcgaag tacaggctat aaccccgccg gatgatatcg ccgcacagct
     3901 tccgcttgca aaacaggctt tggcgcagca gcagtctgcc aataccttcg acttgttgat
     3961 acgttatttc aacggcaaaa tcaaacagac caaaggagcg caatcggtcg acaacggcgg
     4021 cggtcagtaa ttgacacttt tgttgacaaa ataacggtcg gaatattccg gccgttttcc
     4081 catacggcgg aatatgatga gccacaaaaa acaagatgcc ttccaaggat tgatcgacgc
     4141 gctgaaggtt ttacccaacg tcgggccgaa atcggcacag cggatagcgt atcatttgct
     4201 ccaacacaag cgcaaagagg ctgaaaaact ggtggatgcc ttgcagacgg cattgaagca
     4261 ggttcaccat tgcgcgatgt gcaacacgtt ttgcgaaggc ggattgtgcg atatttgtgc
     4321 cgatgaaaca cgcgacgggc ggcggctgat ggtggtgcat atgcctgccg acgtgtcgaa
     4381 tatggaagtg gcaaactgcc acgacgggct gtatttcgtc ctgatggggc aaatcaatac
     4441 ggcattggga atggacgtat ccgccatcgc attggacagg ctggcgcaac ggctgggcgg
     4501 cggggaagtc gaagaaatca ttattgcaac cgcttttacc gcagaaggca atgcgacggc
     4561 gtatgtcctg tccgagtttt ttaaaaacct gccttacaaa gtcagcaggc tgtcgcaggg
     4621 catccccttg ggcggcgaat tggaatatgt cgatgcggga acgctggcgc aggcggtgta
     4681 cgaacgccgc ctgattaaag aaggcggggc ataacgccgc caatgcaaaa tgccgtctga
     4741 agccttcaga cggcattttt ccgggcggtt tacttgcagt ttagcccttg aatctgcaat
     4801 gcgcctccgt tgccgacttt gaacacgcac tgcgtcgcgc tgctgccgga cacggtaatg
     4861 ctgccgctgc cgccgttcag gctgataggt tccttaacac ccataccgac tgccgttgcc
     4921 gccagctttt gcgcgtcggc ttcggaataa ggcgtgttgt tggcatcgat tttgatttct
     4981 ttggcgtagg cagccgcagc ggcggataag gcgatggcgc acaaagcaaa agcagttcgg
     5041 atattcattg ttttcctcaa gaaacggttt t
//
LOCUS       DAWHSK010000142         5065 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.142, whole genome shotgun sequence.
ACCESSION   DAWHSK010000142 DAWHSK010000000
VERSION     DAWHSK010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5065
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.142"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..1548
                     /locus_tag="WIC03_001469"
     CDS             <1..1548
                     /locus_tag="WIC03_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEZ3883948.1"
                     /translation="FGKTEVALRAAFVAVMGGKQVAVLAPTTLLVEQHAQNFADRFAD
                     FPVKVASLSRFNNSKATKAALEGMADGTVDIVIGTHKLVQDDIKFKNLGLVIIDEEHR
                     FGVRQKEQLKRLRANVDILTMTATPIPRTLSMALEGLRDFSLITTAPSRRLAVKTFVK
                     PFSEGSVREAVLRELKRGGQVFFLHNEVDTIENMRERLETLLPEARIGVAHGQLRERE
                     LEQVMRDFLQQRFNVLLCSTIIETGIDIPNANTIIINRADKFGLAQLHQLRGRVGRSH
                     HQAYAYLLTPEYITKDAEKRLDAIAAADELGAGFTLAMQDLEIRGAGEILGEGQSGEM
                     MQVGFTLYTEMLKQAVRDLKKGRQPDLDAPLGITTEIKLHSPALLPESYCPDIHERLV
                     LYKRLAVCETVQQINAIHEELVDRFGLPEQPVKTLIESHHLRLMAKELGINAIDAAGE
                     AVTVTFGKNNNIDPTEIILLIQNDKKYRLAGADKLRFTAEMENIEVRINTVKNVLKTL
                     QNRCLPK"
     gene            1697..2080
                     /locus_tag="WIC03_001470"
     CDS             1697..2080
                     /locus_tag="WIC03_001470"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HEZ3883949.1"
                     /translation="MFRTMLGGKIHRATVTEADLNYVGSITVDQDLLDAAGIYPNEKV
                     AIVNNNNGERFETYTIAGKRGSGVICLNGAAARLVQKGDIVIIMSYVQLSEPEIAAHE
                     PKVVLVDGNNKIRDIISYEPPHTVL"
     gene            2102..2944
                     /gene="kdsA"
                     /locus_tag="WIC03_001471"
     CDS             2102..2944
                     /gene="kdsA"
                     /locus_tag="WIC03_001471"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEZ3883950.1"
                     /translation="MDIKINDFTLGNNSPFVLFGGINVLESLDSTLQTCAHYVEVTRK
                     LGIPYIFKASFDKANRSSIHSYRGVGLEEGLKIFEKVKAEFGIPVITDVHEPHQCQPV
                     AEVCDVIQLPAFLARQTDLVAAMAETGNVINIKKPQFLSPSQMKNIVEKFHEAGNGKL
                     ILCERGSSFGYDNLVVDMLGFGVMKQTCGNLPVIFDVTHSLQTRDAGSAASGGRRAQA
                     LDLALAGMATRLAGLFLESHPDPKLAKCDGPSALPLHLLEDFLIRIKALDDLIKSQPI
                     LTIE"
     gene            2959..3399
                     /locus_tag="WIC03_001472"
     CDS             2959..3399
                     /locus_tag="WIC03_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HEZ3883951.1"
                     /translation="MMQTFRKISLYAATLWLGMQIMAGYIVAPVLFKMLPKMQAGEIA
                     GVLFDILSWSGLAVWGAVLAAAFAALTRRQTALLLFLLSALAANRFLITPVIEALKYG
                     HENWLLSALGGSFGMWHGISSIVFMAAALLSIYLSWRLYGKNAV"
     gene            3444..4730
                     /gene="eno"
                     /locus_tag="WIC03_001473"
     CDS             3444..4730
                     /gene="eno"
                     /locus_tag="WIC03_001473"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEZ3883952.1"
                     /translation="MSAIVDIFAREILDSRGNPTVECDVLLESGVMGRAAVPSGASTG
                     QKEALELRDGDKSRYLGKGVLKAVDHVNNQIAQALIGIDASEQSYIDQIMIELDGTEN
                     KGNLGANATLAVSMAVARAAAEDAGLPLYRYLGGAGPMSLPVPMMNVINGGEHANNSL
                     NIQEFMIMPVGAKSFREALRCGAEIFHALKKLCDSKGFPTTVGDEGGFAPNLNSHKEA
                     LQLMVEATEAAGYKAGEDVLFALDCASSEFYKDGKYHLEAEGRSYTNAEFAEYLEGLV
                     NEFPIISIEDGMDENDWEGWKLLTEKLGGKVQLVGDDLFVTNPKILAEGIEKGVANAL
                     LVKVNQIGTLSETLKAVDLAKRNRYASVMSHRSGETEDSTIADLAVATNCMQIKTGSL
                     SRSDRMAKYNQLLRIEEELAEAADYPSKAAFYQLGK"
     gene            4744..5022
                     /gene="ftsB"
                     /locus_tag="WIC03_001474"
     CDS             4744..5022
                     /gene="ftsB"
                     /locus_tag="WIC03_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HEZ3883953.1"
                     /translation="MKWVTVVLSFALVCCQYSLWFGKGSIGRNSSLREQIAVQEEKNQ
                     TLALRNHSLAAEVYDLENGQEAISEIARVELGYIQDGETFYRLIRHNR"
BASE COUNT         1328 a         1484 c         1222 g         1031 t
ORIGIN      
        1 ttcggcaaaa ccgaagtcgc cctgcgcgcc gcatttgtgg cggtgatggg tggcaaacag
       61 gtcgccgtac tcgccccgac cacgcttctg gtcgagcagc acgcgcaaaa ctttgccgac
      121 cgtttcgccg atttccctgt gaaagtcgcc agcctttcgc gtttcaacaa cagcaaagcc
      181 actaaagccg cgctggaagg catggcggac ggcacggtcg acatcgttat cggcacgcac
      241 aaactggtgc aggacgacat caaattcaaa aacttaggtt tagtgattat cgacgaagaa
      301 caccgcttcg gtgtccgcca aaaagagcag ctcaaacgcc tgcgcgccaa tgtggacatc
      361 ctcaccatga ccgccacgcc gattccgcgt actttaagta tggcgttgga aggactgcgc
      421 gacttctcgc tgattaccac cgcgcccagc cgccgcctcg ccgtcaaaac ctttgtcaaa
      481 ccctttagcg aaggcagcgt gcgcgaagcc gtgttgcgcg aactcaaacg cggaggacag
      541 gtatttttcc tgcacaatga agtagatacg attgaaaata tgcgcgagcg gctggaaacc
      601 ctgctgcccg aagcccgcat cggcgtggcg cacggacaac tgcgcgagcg cgagctggaa
      661 caagtcatgc gcgacttttt gcagcaacga tttaacgtgt tgctctgctc caccatcatc
      721 gaaaccggca tcgacatccc caacgccaac accatcatca tcaaccgcgc cgacaaattc
      781 gggctggcgc aactgcacca gcttcgcggg cgcgtcggcc gcagccacca tcaagcctac
      841 gcctacctgc tcacgcccga atacatcacc aaagacgcag aaaaacgcct cgacgccatc
      901 gcggcggcag acgaactcgg cgcaggtttc accctcgcca tgcaggattt ggaaatccgc
      961 ggcgcaggcg aaatcctcgg cgaagggcag tccggtgaaa tgatgcaggt cggcttcacg
     1021 ctctacaccg aaatgctcaa acaggccgtg cgcgacctca aaaaaggccg ccaacccgac
     1081 ctcgatgcac cgttgggcat taccaccgaa atcaaactgc acagccccgc cctgctgccc
     1141 gaaagctact gccccgacat ccacgaacgg ctcgtcctct acaaacgcct cgccgtctgc
     1201 gaaaccgtac agcaaatcaa tgccatacac gaagaactcg tcgaccgctt cggactgccc
     1261 gagcaacccg tcaaaaccct tatcgaaagc caccacttac ggcttatggc aaaagaattg
     1321 ggtatcaatg ccattgatgc ggccggcgaa gcggtaacgg taacctttgg caaaaacaac
     1381 aatattgatc caaccgaaat catcctgctg attcagaacg acaaaaaata ccgccttgcc
     1441 ggcgccgata agctgcggtt taccgcagag atggaaaata tcgaggtcag aatcaacacc
     1501 gtaaaaaacg ttttaaaaac cttgcaaaac agatgcctgc ccaaataaag ccgacaccgc
     1561 aatgctgtct gaaacaccgt tttccttgtc cgaaagccgc cattatgaat ttgaaggaaa
     1621 ctccactata atacggcatt cagattttca gacggcatcg cgcccgtcaa accgcacaca
     1681 aaccaaaagg aaatacatgt tccgtactat gcttggcgga aaaatccacc gcgccaccgt
     1741 taccgaagcc gatttaaact atgtcggcag cattaccgtc gatcaagacc tgttagacgc
     1801 ggcaggcatc taccccaacg aaaaagtcgc catcgtcaac aacaacaacg gcgaacgttt
     1861 tgaaacctat accattgcag ggaaacgcgg cagcggcgtg atttgtctga acggtgctgc
     1921 ggcaaggctg gtgcagaaag gcgatatcgt catcatcatg tcttacgtcc aactctccga
     1981 acccgaaatc gccgcgcatg aacccaaagt cgtcttggta gacggaaaca acaaaatccg
     2041 cgacatcatc tcctatgagc cgccgcacac cgtgctgtaa ttccgcaaac ggacatcgat
     2101 tatggatatt aaaatcaacg acttcaccct cggcaacaac tcgcccttcg tcctattcgg
     2161 cggcatcaac gttttggaaa gcttggattc caccctccaa acctgtgcgc attacgtcga
     2221 agttacccga aaactcggta ttccctatat ctttaaagcc tctttcgaca aggcaaaccg
     2281 ttcctccatc cattcttatc gcggcgtagg cttggaagaa ggcttaaaga tttttgaaaa
     2341 agtcaaagca gagttcggca tccccgtcat taccgacgta cacgaacccc atcagtgcca
     2401 acccgtcgcc gaagtgtgcg atgtcatcca gcttcccgcc tttcttgcgc ggcagaccga
     2461 tttggtggcc gcaatggcgg aaacgggcaa tgtcatcaac atcaaaaaac ctcagttcct
     2521 cagcccctct caaatgaaaa acattgtgga aaaattccac gaagccggca acgggaaact
     2581 gattttatgc gaacgcggca gcagcttcgg ctacgacaac ctcgttgtcg atatgctcgg
     2641 tttcggcgtg atgaaacaga cttgcggcaa cctgccggtt attttcgacg ttacccattc
     2701 cctgcaaacc cgcgatgctg gttctgccgc atccggcggt cgtcgcgcac aagctttgga
     2761 tttggcactt gcaggcatgg caacccgcct tgccggcctg ttcctcgaat cgcaccccga
     2821 tccgaaactg gcaaaatgcg acggccccag cgcgctgccg ctgcaccttt tagaagattt
     2881 tttaatccgc atcaaagcat tggacgattt aatcaaatca caaccgattt taacaatcga
     2941 gtaacacggt ttcgccttat gatgcagact ttccgaaaaa tcagcctgta tgccgcaacc
     3001 ttatggctcg gtatgcagat tatggcgggt tatatcgtcg cgccggtgct gttcaaaatg
     3061 ctgcccaaaa tgcaggcggg cgaaattgcc ggcgtattgt tcgacatcct ctcttggagc
     3121 gggcttgccg tttggggcgc ggtactggct gccgcctttg ccgccctaac ccggcggcaa
     3181 accgccctgc tgcttttttt attgtccgcc cttgccgcca accgattctt gattacaccc
     3241 gttatcgagg cgctgaaata cgggcatgag aattggctgt tgtcggcttt gggcggatct
     3301 ttcggaatgt ggcacggcat ttccagtatt gtttttatgg cggccgccct actttcgatt
     3361 tacttaagtt ggcggctcta cggcaaaaat gccgtctgaa gcctaccgtt ttacctcacg
     3421 tcactttaat ttggagaata tttatgagcg caatcgttga tatttttgcc cgcgaaattt
     3481 tggactcacg cggcaaccca accgtagaat gcgacgtact gttggaatcc ggcgtcatgg
     3541 gacgcgcagc cgtaccaagc ggcgcatcaa ccggtcagaa agaagcttta gaacttcgcg
     3601 acggcgacaa atcccgttac ttgggcaaag gcgtattgaa agccgttgac cacgtcaaca
     3661 accaaatcgc gcaagccctc atcggtatcg atgccagcga acaatcttat atcgaccaaa
     3721 tcatgatcga attggacggt actgaaaaca aaggtaactt gggtgccaac gcgactttgg
     3781 ccgtttctat ggccgtagca cgcgcagccg ctgaagatgc agggctgccg ctttaccgct
     3841 acttgggcgg cgcaggtccg atgtccctgc ccgtaccgat gatgaacgtc atcaacggcg
     3901 gcgaacacgc caacaacagc ctgaacatcc aagagtttat gattatgccc gtcggcgcaa
     3961 aatctttccg cgaagcgttg cgctgcggtg cggaaatttt ccacgccttg aaaaaactgt
     4021 gcgacagcaa aggcttcccg accacagtcg gcgacgaagg cggtttcgcc cccaacctga
     4081 acagccacaa agaagccctg caactgatgg ttgaagccac cgaagccgcc ggctacaaag
     4141 cgggcgaaga cgtattattc gcattggact gcgcgtccag cgagttctac aaagacggca
     4201 aataccactt ggaagccgaa ggccgctcct acaccaacgc ggaatttgcc gaatatctgg
     4261 aaggcctggt caacgagttc cccatcatct ccatcgaaga cgggatggat gaaaacgact
     4321 gggaaggctg gaaactgctg accgaaaaac tgggcggcaa agtccaactc gttggcgacg
     4381 acctcttcgt taccaacccg aaaatccttg ccgaaggcat tgaaaaaggc gtggcaaacg
     4441 cactattggt caaagtcaac caaatcggta ctttgagtga aaccctgaaa gccgtcgact
     4501 tagccaaacg caaccgctac gccagcgtaa tgagccaccg ctccggcgaa accgaagaca
     4561 gcaccattgc cgacttggca gtcgccacca actgtatgca gatcaaaacc ggttctttga
     4621 gccgttccga ccgcatggcg aaatacaacc aactgctgcg tatcgaggaa gaattggcgg
     4681 aagccgccga ctaccccagc aaagccgcat tctaccaact gggcaaataa aaaaggttaa
     4741 ggtatgaagt gggtaactgt cgttttatcc ttcgcacttg tctgttgcca atacagcctc
     4801 tggttcggca aaggcagcat cggacgcaac agcagtctga gagaacagat tgccgttcaa
     4861 gaagaaaaaa accagacact cgccctacgc aatcattccc ttgccgccga agtctatgat
     4921 ttggaaaacg gtcaagaagc catttcggaa atcgcccggg tagaactggg ttatatccaa
     4981 gacggtgaaa ccttttaccg actcatcagg cataaccggt aataccgtca aaaagccgtc
     5041 cgaaccaatg ttcggacggc tttta
//
LOCUS       DAWHSK010000143         5040 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.143, whole genome shotgun sequence.
ACCESSION   DAWHSK010000143 DAWHSK010000000
VERSION     DAWHSK010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5040
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.143"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..254
                     /locus_tag="WIC03_001475"
     CDS             <1..254
                     /locus_tag="WIC03_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEZ3883954.1"
                     /translation="TAGTIAMARTTAPDSATSQFFINLADNASLDYKNGQYGYTVFGR
                     VESGMNTVSKIARVKTATRGFYQNVPVQPVKIRRVVVGQ"
     gene            370..2418
                     /locus_tag="WIC03_001476"
     CDS             370..2418
                     /locus_tag="WIC03_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEZ3883955.1"
                     /translation="MLPTQPNPTQPNPTQPNPSPDLSDTAGANTEAIYTSDGITANST
                     QLEQLKKLFPACFDADGNFLIDRLQAEIAPQTDIGREFYEMNWLGKSYARLLRNLPPE
                     TLISEDKTHNAKPENAGSQNLLIRGDNLEVLKHLKNAYANSVKMIYIDPPYNTGSDGF
                     VYQDDRKFTPAELALLANIDEDEAARILDFTDKGSNSHSAWLTFMYPRLYIARELLKD
                     DGVIFISIDDNEAAQLKLLCDEVFGEGNFVAQLPWRKRTAKSDVPFGISQDYEWIFVF
                     AKSCQFIAATKGKERRYYETDDFPDRPWRTHDLTKQTTAAERPNSFFTMVDPKTGKKY
                     PANPNATWRVTKDTFQDYYNKGKIVFPDDYDFLNISNPVMRYFKDDDMKKAGEDFGKV
                     AVSSRLPENVGTLADAVAEYLAIFSRTLPENIGMTKEGTKEITDLFGSKIFTFPKPSQ
                     LIKFLVSISSKSNDLILDFFAGSGTTAHAVMQLNAEGQNGNRRYICVQLPEKTAEKSE
                     ARKAGYPTIFDITKARIEKAAAKIRVEHPDYTGDSGFKIFQTADNFRQHPDKDFSPEQ
                     PDLPLNDELSEEQLQTLLTTWTLYDGAALTTPVEPVRLGDYTAYLCEKRLYLLNKGFT
                     SVDLLAFIRKLDDDAAFNPNRVIVFGSNMASAMQHELDQAVRGYANKKEIELNVIIRV
                     "
     gene            2431..>5040
                     /locus_tag="WIC03_001477"
     CDS             2431..>5040
                     /locus_tag="WIC03_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III restriction-modification system
                     endonuclease"
                     /protein_id="HEZ3883956.1"
                     /translation="MSGFNYEKNQPHQMRAVSAVLGVFDGATPKYRAADENPELLFAA
                     KQYADNILKVQSQNGIDGRFPDRSDDQNILDISMETGTGKTYTYTQTMFELHRWLGVF
                     KFIVVVPTLSIKAGTRQFLQSKALAEHFEQDFGGDYEGVRLKTYVVESAKKNKGKKSN
                     APITIEQFVKAENKKEIHVLLINAGMVNSPSMTDTGDKALKDLFDNSVDALAAVRPFV
                     IVDEPHKFPTRDSAKTWGNIKRLKPQYILRYGATFNDEYYNLLYRLTAVDAFNDGLVK
                     GVRVFQEEMQGGMDAAVKLVSSDGKEAKFELNEKDKKQTFKLAKGEDLAKIHPAVSDL
                     KIDKMNKTVVVLSNGLELKTGAVINPYSYSQTVQDAMMQRAVAENFKLERALLAERAP
                     QPKIKPLTLFFIDDIAGYRSGNELSGSLKDKFESWIHAEAARRLKTESDPFYRDYLQK
                     TLDDVSACHGGYFSKDNTDSDDRIEQEINEILHDKEKLLSLDNPRRFIFSKWTLREGW
                     DNPNVFQICKLRSSGSTTSKLQEVGRGLRLPVNELMARVRDVPYKLNYFVDSSEKDFV
                     KQLVGEINDNSFQEEISKKFTEELKQKILQKYPDIKPLVLVNQLFSDGIIDDNENFTK
                     DGYDKLKAAYPEAFPKGLDKGKVSNAKDEGKDTIIMREGKYEELKALWELIHHKAVLQ
                     YKIKDEAEFVDLFTAYLRENAAKFPQAGIRTAVNEAYINNGLMLSRRIDSIEDEDFIR
                     FNTMTYREFLEKLAQTAKIRMQTLHQAFYRVRDELNIGDFLNMQTIAQIKNGFNRFLL
                     HHSFHKFELDYRLVGSKIHPTKFTDKDGKPRAVKKADLGRFEDTEHRPAAGYLFDEIF
                     YDSDIEHENVANNQ"
BASE COUNT         1522 a         1228 c         1184 g         1106 t
ORIGIN      
        1 acaccgccgg caccatcgcc atggcgcgga cgacagcccc cgattccgcc accagccaat
       61 tctttatcaa tctggcggac aacgcttcgc tcgactacaa aaacggacaa tacggctata
      121 ccgttttcgg cagggtcgaa agcggcatga acaccgtttc caaaatcgcc cgcgtcaaaa
      181 ccgccacgcg cggcttttat caaaacgtac ccgtacagcc cgtcaaaatc cgtcgcgttg
      241 ttgtcgggca gtaacacgca gacagacgtt cagacggcgt cgcccgtttc ccaaaaaacg
      301 ccgtttaaag taaaaaaata ttttaaaaca gacagttgat attgacaaat tcaaaccgag
      361 gattttaaaa tgctgccaac ccaacccaac ccaacccaac ccaacccaac ccaacccaac
      421 ccaagccctg atctaagcga cacagccggg gcgaacactg aggcaatcta cacttcagac
      481 ggcattaccg ccaactctac ccaactcgaa cagctcaaaa aactgtttcc cgcctgtttt
      541 gacgcagacg gaaatttcct tatcgacaga ttacaagccg aaattgcgcc acagaccgac
      601 atcggacgcg aattttacga aatgaactgg ctgggcaaat catatgcccg cctgcttcgc
      661 aacctgccgc ccgaaaccct gataagtgaa gacaaaacgc acaacgccaa gcccgaaaat
      721 gcaggcagcc aaaatctgct gattcgcggc gataatctgg aagtgttgaa acacttaaaa
      781 aacgcctacg caaacagcgt gaagatgatt tacatcgacc cgccctacaa caccggatcg
      841 gacggctttg tctatcagga cgaccgcaaa ttcacacccg ccgaacttgc cctcctggcg
      901 aatattgacg aagacgaagc cgcgcggatt ttagatttca ccgacaaagg ctcaaactcg
      961 cacagcgcgt ggctgacctt tatgtatccg cgcctgtata tcgcccgcga actgttaaag
     1021 gacgacggcg tgatttttat ctcgattgac gataacgaag cggcgcagtt gaaattgttg
     1081 tgtgatgaag tgtttgggga agggaatttt gttgcacaat tgccttggcg aaaaagaaca
     1141 gctaaatcag atgtgccttt tggtatttcg caggattatg aatggatatt tgtattcgca
     1201 aaatcttgcc aatttattgc agcaactaaa ggcaaggaac gacgctatta tgagactgat
     1261 gatttccccg atcgtccttg gcgtacccac gacttaacga aacaaacaac tgcggcggaa
     1321 aggccaaata gttttttcac aatggttgat cccaagacag gaaaaaaata tccagcaaat
     1381 ccaaatgcaa cttggcgtgt aaccaaagat acatttcaag attattataa taaaggaaaa
     1441 atcgttttcc ctgatgatta tgatttctta aatatcagca atccagttat gcgttacttt
     1501 aaagatgacg acatgaaaaa agctggcgag gattttggca aagtagctgt aagcagtaga
     1561 ttacctgaaa atgttggtac attagccgat gcagtagccg aatatttggc tatttttagt
     1621 aggacgctac ctgaaaatat cggaatgact aaagaaggca caaaagaaat cacagattta
     1681 tttggtagca aaatatttac cttccccaag cctagtcaat tgattaaatt tttagtttca
     1741 ataagttcaa agagtaatga cctaatccta gacttcttcg caggcagcgg cacaaccgcc
     1801 cacgccgtga tgcagcttaa cgccgaagga caaaacggta accgccgcta tatctgtgta
     1861 cagcttcccg aaaaaaccgc tgaaaaatcc gaagcccgta aagcaggcta cccgaccatc
     1921 ttcgacatca ccaaagcccg catagaaaaa gccgccgcca aaatccgcgt cgaacatccc
     1981 gattacacgg gcgattcggg cttcaaaatc tttcaaacgg cagacaattt caggcagcat
     2041 ccggacaagg atttttcgcc cgaacaaccg gatttgccgc ttaacgatga attaagcgaa
     2101 gaacagctgc aaacgcttct gaccacttgg acactgtatg acggggcggc actgaccacg
     2161 ccggttgagc ctgtgcggtt aggggattac acggcgtatt tgtgcgaaaa gcggctgtat
     2221 ctgcttaaca agggttttac ttccgtcgat ttgttggcgt ttatccgcaa gctggacgac
     2281 gatgcggctt tcaatcccaa ccgcgtgatt gtattcggca gcaatatggc aagcgccatg
     2341 cagcacgaac ttgaccaggc ggttcgcggt tatgccaata aaaaagaaat tgagttaaac
     2401 gtgattatca gggtttgacg gagaaagttt atgagtggtt ttaattacga gaaaaaccag
     2461 ccgcaccaaa tgcgggcggt ttcggcggtt ttgggcgtgt ttgacggggc aacgcccaaa
     2521 tatcgggcgg cagacgaaaa tcccgaactt ttgtttgctg caaaacaata cgcagacaat
     2581 atcctgaaag tgcaaagcca aaacggcata gacggccgat tccccgaccg ttcggacgac
     2641 caaaatatcc ttgatatttc catggaaacg ggcacgggca aaacctatac ctacacacaa
     2701 accatgttcg agctgcaccg ttggctgggc gtgttcaaat ttatcgtggt cgtgccgact
     2761 ttgtccatta aggcgggaac gcggcagttt ttgcaaagca aggctttggc agagcatttt
     2821 gaacaggatt tcggcggcga ttatgaaggc gtacgcctga aaacctatgt ggtggaaagc
     2881 gcgaaaaaga ataagggcaa aaagtccaat gcgcccataa caatcgagca atttgtcaaa
     2941 gcggaaaaca aaaaggaaat ccatgtgctg ctgattaacg cgggcatggt taattcgccg
     3001 tccatgaccg atacgggcga caaggcattg aaggatttgt ttgacaattc cgttgatgca
     3061 ttggctgccg tgcgcccgtt tgtgattgtg gacgaaccgc ataaattccc gacccgagat
     3121 agcgcgaaaa cgtggggcaa tatcaaacgc ttaaaaccgc aatatatttt gcgctacggt
     3181 gcgacattta acgatgaata ttacaacctg ctttaccgtc tgacggctgt agacgcgttt
     3241 aacgacgggc tggtcaaagg cgtgcgcgtg tttcaggaag aaatgcaggg cggcatggat
     3301 gcggcggtaa aactggtgtc gtcggacggc aaagaagcga aatttgaatt aaacgaaaag
     3361 gacaaaaagc agacgttcaa actggcaaaa ggcgaagatt tggcgaaaat ccatcccgct
     3421 gtttcggatt tgaaaatcga caaaatgaat aaaaccgtgg tggtgttaag taacggcttg
     3481 gagttgaaaa cgggtgccgt catcaaccct tattcctatt cgcaaacggt gcaggatgcg
     3541 atgatgcaga gggcggttgc cgagaatttc aagctggaac gtgcgctttt ggcagaacgc
     3601 gcgccacagc ccaaaatcaa gccgctgaca ctgtttttta ttgacgatat tgcgggctac
     3661 cgcagcggca acgagctttc aggcagcctg aaagataaat ttgaaagctg gattcacgcg
     3721 gaagcggcac gccgcctgaa aacggaaagc gacccgtttt accgcgatta cctgcaaaag
     3781 acgttggacg atgtatccgc ctgccacggc ggttattttt ccaaagacaa tacagacagc
     3841 gacgatagaa tcgagcagga aatcaatgaa atcctgcacg ataaggaaaa actgttgtct
     3901 ttggacaacc cgcgccgctt tattttttcc aaatggacgc tgcgcgaagg ctgggacaat
     3961 cccaacgttt tccagatttg caaactgcgt tccagcggca gcacgacttc caagctgcaa
     4021 gaagtcggac gcggcctgcg cctgccggta aacgagctga tggcgcgggt gcgcgatgtg
     4081 ccgtacaaac tgaattattt tgtcgatagc agcgaaaaag actttgtgaa gcagcttgtc
     4141 ggcgaaatca acgataattc ttttcaggaa gaaatctcca aaaagtttac cgaagagctg
     4201 aaacaaaaaa tattgcaaaa ataccccgat atcaaaccgc tggtattggt aaaccaactg
     4261 ttttcagacg gcatcattga cgacaatgaa aactttacca aagacggcta tgacaaatta
     4321 aaagccgcct atcccgaagc cttccccaaa ggtttggaca aaggcaaagt cagcaacgcc
     4381 aaagacgagg gcaaagacac catcatcatg cgcgaaggca aatatgaaga actcaaggcc
     4441 ttgtgggagc tgattcacca taaagccgtt ttgcagtaca aaatcaagga tgaagccgaa
     4501 tttgtcgatt tatttaccgc ctatttgcgt gaaaacgccg ccaaattccc gcaggcaggc
     4561 atacgcacgg cggtaaacga agcttatatc aacaacgggc ttatgctttc ccgccgcata
     4621 gacagtattg aagatgaaga ttttatccgt ttcaacacaa tgacttaccg agagtttctg
     4681 gaaaaactgg cacaaacggc aaaaatccgc atgcagactt tgcatcaggc gttttaccgc
     4741 gtccgcgacg aactgaacat tggcgatttt ttgaatatgc agaccatcgc ccaaatcaaa
     4801 aacggcttca accggttttt gcttcatcat tccttccata aattcgaact ggattaccgg
     4861 cttgtcggca gcaaaatcca tccgaccaaa tttaccgata aagacggcaa accgcgcgcg
     4921 gtgaaaaaag cagatttggg aagatttgaa gatacggagc accggcctgc cgccggctat
     4981 ctcttcgacg agattttcta cgattcggat atagaacatg aaaatgtcgc caacaaccaa
//
LOCUS       DAWHSK010000144         4947 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.144, whole genome shotgun sequence.
ACCESSION   DAWHSK010000144 DAWHSK010000000
VERSION     DAWHSK010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4947
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.144"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            102..1007
                     /gene="glyQ"
                     /locus_tag="WIC03_001478"
     CDS             102..1007
                     /gene="glyQ"
                     /locus_tag="WIC03_001478"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEZ3883957.1"
                     /translation="MLTFQQIIFKLQTFWADKGCTVIQPFDMEVGAGTSHPATCLRAL
                     GPEPWFAAYVQPSRRPKDGRYGDNPNRLQHYYQFQVALKPAPANIQDLYLDSLRELGI
                     DPKVHDIRFVEDDWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGIDCTPVLGEITY
                     GIERLAMYLQGVENVYDLVWAKTLDGNTVTYGDVYHQNEVEQSTYNFEYSDADWLLRQ
                     FNDYEAQAKRLLAEENAALALPAYELVLKAGHTFNLLDARGAISVTERATYIGRIRAL
                     SRAVAQKYVESREKLGFPLMKANAA"
     gene            1084..1494
                     /locus_tag="WIC03_001479"
     CDS             1084..1494
                     /locus_tag="WIC03_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HEZ3883958.1"
                     /translation="MPVYFRTPSRKRLHKMNRRQHKKLHLGEFQTLIFGVQGSLHQAE
                     DDAEAFDRFMDELIAYLAANGLEMAGGGDCGDFSCLFQKSGSRGLGETEKKAIISWLA
                     GREDVATLHATPLVDGFYGDDGWENYSETYKQAD"
     gene            1515..3578
                     /locus_tag="WIC03_001480"
     CDS             1515..3578
                     /locus_tag="WIC03_001480"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEZ3883959.1"
                     /translation="MMTQTLLIELLTEELPPKALNNLGNHFAAAVAEGLEKAQLVDGA
                     AEFTAYASPRRLAVQVKNVKAVQADQKIVKKGPAVANAMKDGAPTKALEGFARGAGAK
                     IEDLTIVHDGKQDVYAYEYVQTGKPLGELLEDIINQAVKKLPIPKVMRWGSSTFTFVR
                     PVHGLIVLHGGDIVNVSVLGLQSSNQTLGHRFLSNGEITIENADSYAEQMRGQGKVVA
                     SFAERKAAIQTALEGQARRLNATVAADEALLDEVTALVEYPVVLEAGFEEHFLAVPQE
                     CLILTMQQNQKYFPLLDQNGKLMNRFLLVSNLQTADPSHIIQGNERVLRARLSDAEFF
                     YKQDQKATLESRLPKLANVVYHNKIGSQAERIERLQSIAAHIAKALGADAAAAERAAR
                     LAKADLVTEMVGEFPELQGTMGKYYARLDGETEEIAEAIEQHYQPRFAGDNLPNGKVA
                     TAVALADKLETLVGIWGIGLIPTGDKDPYALRRAALGILRMLMQYGLDVNELIQTAFD
                     SFPKGLLNEKTPSETADFMQARLAVLLQNDYPQDIVAAVLAKQPRRLDDVVAKLQAVA
                     AFKQLPEAAALAAANKRVQNLLKKADAELGAVNESLLQQDEEKALFAAAQGLQPKIAA
                     AVAEGNFQTALSELASVKPQVDAFFDGVMVMAEDAAVKQNRLNLLNRLAEQMNAVADI
                     ALLGE"
     gene            3595..3996
                     /locus_tag="WIC03_001481"
     CDS             3595..3996
                     /locus_tag="WIC03_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEZ3883960.1"
                     /translation="MPSEAFRRHRAYRENKLQPLVSVLICAYNAEKYFAQSLAAVVNQ
                     TWRNLEILIVDDGSTDGTLAIAKDFQKWDSRIKILAHDRLLNRKQQRRDSPANTFKHR
                     LIRALTKISREREKRRQRREQLIGKIIVPFQ"
     gene            4008..4940
                     /locus_tag="WIC03_001482"
     CDS             4008..4940
                     /locus_tag="WIC03_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEZ3883961.1"
                     /translation="MDIVFAADDNYAAYLCVAAKSVEAAHPDTEIRFHVLDAGISEAN
                     RAAVAANLRGGGNIRFIDVNPEDFAGFPLNIRHISITTYARLKLGEYIADCDKVLYLD
                     IDVLVRDSLTPLWDTDLGDNWLGACIDLFVERQEGYKQKIGMADGEYYFNAGVLLINL
                     KKWRRHDIFKMSCEWVEQYKDVMQYQDQDILNGLFKGGVCYANSRFNFMPTNYAFMAN
                     RFASRHTDPLYRDRTNTVMPVAVSHYCGPAKPWHRDCTAWGAERFTELAGSLTTVPEE
                     WRGKLAVPHRMFSTKRMLQRWRRKLSARFLRKIY"
BASE COUNT         1260 a         1395 c         1289 g         1003 t
ORIGIN      
        1 atgtatgccg tgttttcaaa tcagtctgtc tttgagataa acctatccaa acagcaatgg
       61 aaacagtaaa ataaaccctt tattttatcg aatcataagc catgcttacc ttccaacaaa
      121 tcatcttcaa actgcaaaca ttctgggcag acaaaggctg caccgtcatc caacccttcg
      181 acatggaagt cggtgccggc acatcccatc ccgccacctg cctgcgcgca ctcggccccg
      241 agccttggtt tgccgcctac gtccaaccca gccgccgccc caaagacggc cgctacggcg
      301 acaaccccaa ccgcctgcaa cactattacc aattccaagt cgccctcaag cccgctcccg
      361 ccaatatcca agacctctat ctcgactctc tgcgcgaatt gggcatcgat cccaaagtcc
      421 acgacatccg ctttgtcgaa gacgactggg aaaaccccac cctcggcgcg tggggtttgg
      481 gctgggaagt ctggctcaac ggcatggaag taacccagtt tacctatttc caacaagtcg
      541 gcggcatcga ctgcaccccc gtactcggcg aaatcaccta cggcatcgaa cgcttggcga
      601 tgtacctgca aggcgtggaa aacgtctatg acctcgtttg ggcaaaaacg ctggacggta
      661 atacagtaac ttacggcgac gtgtaccacc aaaacgaagt cgaacaatcc acctacaact
      721 tcgaatacag cgatgccgac tggctgctgc gccagttcaa cgactacgaa gcgcaagcca
      781 aacgcctgct tgccgaagaa aacgccgccc tcgccctgcc tgcctacgag cttgtcctca
      841 aagcggggca tacgttcaac cttttagacg cacgcggcgc gatttccgta actgagcggg
      901 caacttatat tggacgtatt cgtgcattga gccgcgccgt ggcgcaaaaa tatgttgaaa
      961 gccgcgaaaa actgggcttc cctttaatga aggcaaacgc cgcctaaggg ggcgacgatt
     1021 ttttacgttc gggcggcacg ataaggcagc cgtccggaag ccattatatt ttttaggaaa
     1081 atcatgcctg tctatttccg caccccaagc aggaaacgcc tgcacaaaat gaaccgccgc
     1141 cagcataaaa agctgcattt gggcgagttt caaacattga ttttcggcgt tcaaggcagt
     1201 ttgcaccaag ctgaggacga tgccgaagcg ttcgaccgct ttatggacga gctgattgcg
     1261 tatcttgcgg caaacggctt ggaaatggcg ggcgggggcg attgcggcga tttttcgtgc
     1321 ctgtttcaaa aatccggctc ccgaggcttg ggcgaaaccg agaaaaaagc cattatctct
     1381 tggctggcag gtcgcgaaga tgtcgccact ttgcacgcca ccccgctggt tgacggtttt
     1441 tacggggatg acggctggga aaattacagc gaaacctata aacaggcaga ttaaccaaat
     1501 tcaaagaaag ccgaatgatg acccaaaccc ttttaatcga actccttacc gaagaactcc
     1561 cgcccaaagc tctgaataat ctgggcaacc attttgccgc cgccgttgcc gaaggcttgg
     1621 aaaaagcgca actggttgac ggtgcagccg aatttacggc ttatgcctcg ccgcgccgtt
     1681 tggcggttca agtcaaaaac gttaaagccg ttcaggccga ccaaaaaatc gtgaaaaaag
     1741 gccctgccgt ggcgaatgcc atgaaagacg gtgcgccgac caaggctttg gaaggtttcg
     1801 cacgcggtgc gggcgcgaaa atcgaagact tgaccatcgt ccacgacggc aaacaggatg
     1861 tgtacgccta cgaatacgtc caaaccggca aaccgctggg cgaacttttg gaagacatta
     1921 tcaatcaagc cgttaagaaa ctgccgattc cgaaagtgat gcgttggggc agcagcacgt
     1981 ttaccttcgt gcgccctgtt cacgggctga ttgtgctgca cggcggcgac atcgtaaacg
     2041 tcagcgtttt gggtctgcaa agcagcaatc aaaccttggg acaccgcttc ttgtccaacg
     2101 gcgaaatcac cattgaaaac gccgacagct acgccgaaca aatgcgcggg caaggcaaag
     2161 tcgtcgcttc gtttgccgag cgcaaagccg cgattcagac ggcattggaa gggcaggcgc
     2221 gccgtctgaa cgcgaccgtt gccgccgatg aagcattgct ggacgaagta accgcgctgg
     2281 tcgaataccc cgttgttttg gaagccggtt ttgaggaaca cttcctcgcc gtgcctcaag
     2341 aatgcttgat tttgacgatg cagcaaaacc aaaaatactt cccgctgctc gaccaaaacg
     2401 gcaagctgat gaaccgcttc ctgctggtct ccaacctgca aaccgcagac ccgtcgcaca
     2461 tcatccaagg caacgaacgc gttttgcgcg cgcgcctgtc tgatgccgag ttcttctaca
     2521 aacaagacca aaaggcgact ttggaaagcc gcctgcccaa gctggcaaac gtggtttatc
     2581 acaacaaaat cggttcgcaa gccgaacgca tcgaacgcct gcaaagcatt gccgcccaca
     2641 tcgctaaagc gttgggcgca gatgccgccg ccgccgaacg cgccgcgcgt ctggcaaaag
     2701 ccgacttggt aaccgaaatg gtcggcgagt tccctgaatt gcaaggcacc atgggcaaat
     2761 actatgcccg tttggacggc gaaaccgaag aaatcgccga agccatcgag caacactacc
     2821 aaccgcgttt cgccggcgac aatctgccga atggcaaagt tgccaccgcc gtggcactgg
     2881 cggacaaact agaaaccttg gtcggcattt ggggcattgg tctgattccg accggcgaca
     2941 aagaccccta cgccctgcgc cgcgccgcct tgggtatttt gcggatgctg atgcagtacg
     3001 gtttggacgt gaacgaactg attcagacgg cattcgacag cttccccaaa ggtttgctca
     3061 acgaaaaaac gccgtctgaa accgccgact ttatgcaggc gcgccttgcc gtgttgctgc
     3121 aaaacgatta tccgcaagac atcgttgccg ccgtattggc gaaacagccg cgccgtttgg
     3181 acgacgtagt ggcgaaactg caagccgttg ccgcgttcaa acaactgccc gaagccgccg
     3241 cgctcgccgc cgccaacaaa cgcgtgcaaa atctgctgaa aaaagccgat gccgagttgg
     3301 gcgcggttaa cgaaagcctg ctgcaacagg acgaagaaaa agccctcttt gccgccgcgc
     3361 aaggcttgca gccgaaaatc gccgccgccg tcgccgaagg caatttccaa accgccttgt
     3421 ccgaactggc ttccgtcaag ccgcaagtcg atgccttctt tgacggcgtg atggtgatgg
     3481 cggaagatgc cgccgtaaaa caaaaccgtt tgaacctgtt gaaccgtttg gcagagcaga
     3541 tgaacgcggt agccgacatc gcgcttttgg gcgagtaacc gttgtacagt ccaaatgccg
     3601 tctgaagcct tcagacggca tcgtgcctat cgggagaata aattgcagcc tttagtcagc
     3661 gtattgattt gcgcctacaa cgcagaaaaa tatttcgccc aatcattagc cgccgtcgtg
     3721 aatcagactt ggcgcaactt ggagattttg attgtcgatg acggctcgac agacggcaca
     3781 cttgccattg ccaaggattt tcaaaagtgg gacagccgta tcaaaatcct tgcacacgac
     3841 cgcctcctga accgcaaaca gcaaaggcgc gattcccccg ccaacacatt caaacaccgc
     3901 ctgatccgcg ccttgaccaa aatcagcagg gaaagggaaa aacgtcggca aaggcgcgaa
     3961 cagttaatcg gcaagatcat tgtgcctttc caataaaagg agaaaagatg gacatcgtat
     4021 ttgcggcaga cgacaactat gccgcctatc tttgcgttgc ggcaaaaagc gtggaagcgg
     4081 cccatcccga tacggaaatc aggttccacg tcctcgatgc cggcatcagt gaggcaaacc
     4141 gggcggcggt tgccgccaat ttgcgggggg ggggtaatat ccgctttata gacgtaaacc
     4201 ccgaagattt cgccggcttc cccttaaaca tcaggcacat ttccattacg acttatgccc
     4261 gcctgaaatt gggcgaatac attgccgatt gcgataaagt cctgtatctg gatatagacg
     4321 tattggtcag ggacagcctg acgcccttat gggataccga tttgggcgat aactggcttg
     4381 gcgcgtgcat tgatttgttt gtcgaaaggc aggaaggcta caaacaaaaa atcggtatgg
     4441 cggacggcga atattatttc aatgccggcg tattgctgat caacctgaaa aagtggcggc
     4501 ggcacgatat tttcaaaatg tcctgcgaat gggtggaaca atacaaggac gtgatgcaat
     4561 atcaggatca ggacattttg aacgggctgt ttaaaggcgg ggtgtgttat gcgaacagcc
     4621 gtttcaactt tatgccgacc aattatgcct ttatggcgaa caggtttgcg tcccgccata
     4681 ccgacccgct ttaccgcgac cgtaccaata cggtgatgcc cgtcgccgtc agccattatt
     4741 gcggcccggc aaagccgtgg cacagggact gcaccgcgtg gggtgcggaa cgtttcacag
     4801 agttggcggg cagcctgacg accgttcccg aagaatggcg cggcaaactt gccgtcccgc
     4861 accgtatgtt ttcgacaaag cgtatgcttc aaagatggcg cagaaagctg tctgccagat
     4921 tcttacgcaa gatttattga cggggca
//
LOCUS       DAWHSK010000145         4945 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.145, whole genome shotgun sequence.
ACCESSION   DAWHSK010000145 DAWHSK010000000
VERSION     DAWHSK010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4945
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.145"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(11..1225)
                     /locus_tag="WIC03_001483"
     CDS             complement(11..1225)
                     /locus_tag="WIC03_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEZ3883962.1"
                     /translation="MDKFPKSAKLDHVCYDIRGPVHKKALQLEEEGNKILKLNIGNPA
                     PFGFEAPDEILVDVIRNLPTSQGYCDSKGLYSARKAIVHYYQTKGLRDITVDDVYIGN
                     GVSELITMSMQALLNDGDEILIPAPDYPLWTAAATLAGGTVRHYLCDEENGWFPNLAD
                     MEAKITPKTKAIVVINPNNPTGAVYSREILLEIAELARKHGLIIFADEIYDKILYDGA
                     VHHHIAALAPDLLTVTFNGLSKSYRVAGFRQGWMVLNGPKHHAKGYIEGLDMLSSMRL
                     CANTPMQHAIQTALGGYQSINEFILPGGRLLEQRNRAWELVSQIPGVSCVKPMGAMYM
                     FPKIDTEMYRIRDDMKFVYDLLVREKVLLVQGTGFNWIKPDHFRIVTLPYVHQIEEAM
                     GRLARFLQTYRQ"
     gene            complement(1314..1523)
                     /locus_tag="WIC03_001484"
     CDS             complement(1314..1523)
                     /locus_tag="WIC03_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HEZ3883963.1"
                     /translation="MPYVNIKVTGGKEAPTAAQKAELIGGVTELLARVLGKNPETTVV
                     VIDEVDTDNWGIGGKSVSERRKEGR"
     gene            1868..2674
                     /locus_tag="WIC03_001485"
     CDS             1868..2674
                     /locus_tag="WIC03_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HEZ3883964.1"
                     /translation="MKKTLTLLAVSALFATSAHAHRVWVETAHTHGGEYLKADLGYGE
                     FPELEPIAKDRLHIFSKPMQLVTEKGKENMIQRGTYNYQYRSNRPIKDGSYLVIAEYQ
                     PTFWSKNKAGWKQAGIKEMPDASYCEQTRMFGKNIVNVGHESADTAIITKPVGQNLEI
                     VPLDNPANIHVGERFKVRVLFRGEPLPNATVTATFDGFDTSDRSKTHKTEAQAFSDST
                     DDKGEVDIIPLRQGFWKANVEHKADFPDQSVCQKQANYSTLTFQIGHSHH"
     gene            complement(2732..3997)
                     /locus_tag="WIC03_001486"
     CDS             complement(2732..3997)
                     /locus_tag="WIC03_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEZ3883965.1"
                     /translation="MSEALAKETLNPFEIARKQVKTACDRLKTDPAVYEILKSPTRVL
                     EVNFPVKLDDDTVKTFTGYRSQHNNAVGPYKGGVRFHPGVNLDEVKALSIWMTIKCCV
                     AGIPYGGGKGGVTLDPRDYSEAELERIARAYSEAISPLIGEKIDIPAPDVNTNGKIMS
                     WMVDAYENVVKHSAPGVFTGKPVEFGGSLARTEATGYGVNLAAVQALEKLGKDVKGAT
                     YAIQGFGNVGYHTGYYAHQSGAKVVAVSTVDVAIYNENGLDMEALFKEFQEKGFITNE
                     AGYGKEITNAELLALDVDVLAPCALENQLTSENAGKVRAKIVVEGANGPTTPEADVIL
                     RQNGVLVVPDILANCGGVVVSYFEWVQNLQGYYWEFDEVQEKETVVLRRAFRDIWNLA
                     QEYDVDLRTASYMMSIRRVEKAMKLRGWY"
     gene            4404..>4945
                     /locus_tag="WIC03_001487"
     CDS             4404..>4945
                     /locus_tag="WIC03_001487"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HEZ3883966.1"
                     /translation="MNAAIEHVQAVAFDLDGTLCDSVPDLAAAAEAMLEQLGMKPLPA
                     KVVESYVGDGIGKLVHRVLTNDRDREADSELWEKGFVSYMKYYRDHLSVFTRPYPETE
                     AGLALLKSLGIPLAVITNKNEILAVELLKQLGLADYFSLILGGDSLPEKKPSPLPLRH
                     AAEVLGIDVANMVMVGDSRN"
BASE COUNT         1229 a         1373 c         1184 g         1159 t
ORIGIN      
        1 aaccgtcccc ttattggcgg taggtttgca ggaatcttgc cagcctgccc atcgcctctt
       61 caatctgatg gacgtaaggc agcgtaacaa tgcggaaatg gtcgggttta atccaattaa
      121 accccgttcc ctgcaccagc aagacttttt cgcgcaccag caaatcgtaa acgaatttca
      181 tgtcatcgcg gatgcggtac atttcggtat cgattttcgg gaacatatac atcgcgccca
      241 tcggtttgac gcaggacacg ccggggattt ggctgaccag ttcccacgcc ctgttgcgct
      301 gttccaaaag ccgcccgccc ggcaggataa attcgttaat gctctgatag ccgcccaatg
      361 ccgtctgaat cgcgtgctgc atcggcgtat tggcacacaa gcgcatggac gagagcatat
      421 ccaaaccctc gatgtaacct tttgcgtgat gtttcggccc gttgagcacc atccagcctt
      481 ggcggaagcc ggcgacgcgg taggatttgg acaaaccgtt gaacgttacc gtcaaaaggt
      541 cgggggcaag cgcggcgatg tggtggtgaa ccgcgccgtc ataaaggatt ttatcgtaaa
      601 tctcgtcggc gaaaataatc aaaccgtgct tgcgcgccag ttcggcgatt tccaacagga
      661 tttccctgct gtacaccgcg cctgtcggat tattgggatt gatgacgacg atggctttgg
      721 ttttgggcgt gattttggct tccatatcgg caaggttggg gaaccagccg ttttcttcgt
      781 cgcacagata atggcgtacc gtaccgcccg caagcgttgc cgccgccgtc cacaaagggt
      841 agtcgggcgc gggaatcagg atttcgtcgc cgtcgttgag caatgcctgc atagacatcg
      901 taatcagctc ggacacgccg ttgccgatat agacatcatc aaccgtaata tcgcgcaaac
      961 ctttggtctg atagtagtga acaatggctt tgcgggcgga atacagccct ttagaatcgc
     1021 aatagccttg cgaagtcggc aggttgcgga tgacatcgac caagatttca tcaggggctt
     1081 caaagccgaa cggcgcaggg ttgccgatat tgagtttaag gattttattg ccctcctctt
     1141 ccaactgaag ggcttttttg tgaaccggcc cgcgtatgtc gtaacagacg tgatcgagct
     1201 ttgcagactt gggaaattta tccatgatgt gttccgtaaa ttttgggcaa tgggtgggaa
     1261 tgtactcttt ttcacgcgga atttaaagca tcaaaccgag attttcaggc tttttacctg
     1321 ccctctttgc gccgttcgct gacgcttttg ccgcctattc cccagttatc ggtatccact
     1381 tcgtcaatca cgacaaccgt tgtttcggga tttttgccca gcacgcgtgc cagcaattcg
     1441 gttacgccgc cgatcagttc cgctttttgc gcggcagtcg gtgcttcctt gccgccggtt
     1501 actttaatat tgacataagg catgatcttt ctccgtttta aaatattgct atcttatcaa
     1561 acaagttgcc tccgcccaaa cgtccgcttc attttctgaa aaattcaaat cgatatagcg
     1621 gattaacaaa aatcaggaca aggcggcgaa gccgaagaca gtacaaatag cacggaaccg
     1681 attcacttgg tgcttcagca ccttagagag tcgttctctt tgagctaagg cgagccaacg
     1741 ccgtactggt ttttgttaat ccactataca aaaagacagt tttcagacag caaatccgtc
     1801 ttcacacgat acctattttg ttataacata acaaaatctt taacccacac gagacaaagg
     1861 ctgcaccatg aagaaaacat tgacactgct cgccgtttcc gccctatttg ccacatccgc
     1921 ccacgcccac cgcgtctggg tcgaaaccgc ccacacgcac ggcggcgaat accttaaagc
     1981 cgacttgggc tacggcgaat ttcccgaact cgaacccatc gccaaagacc gcctgcacat
     2041 cttcagcaaa ccgatgcagc tggttaccga aaaaggcaag gaaaacatga ttcaacgcgg
     2101 cacatacaac taccagtacc gaagcaaccg tcccattaag gacggcagtt acctcgtcat
     2161 cgccgaatat cagcctactt tctggtcaaa aaacaaagca ggctggaaac aggcgggcat
     2221 caaagaaatg cctgacgcaa gctattgcga acaaacccgt atgttcggca aaaacatcgt
     2281 caacgtcgga cacgaaagcg cggacaccgc catcatcacc aaaccggtcg gacaaaactt
     2341 ggaaatcgtc ccgctggaca atcccgccaa cattcacgta ggcgaacgct tcaaagtccg
     2401 cgttctgttc cgtggcgaac cgctgcccaa tgccaccgtt accgccacct ttgacggctt
     2461 cgacaccagc gaccgcagca aaacgcacaa aaccgaagca caggctttct ccgacagcac
     2521 agacgacaaa ggcgaagtgg acatcatccc cttgcgccaa ggcttctgga aagccaatgt
     2581 cgaacacaaa gccgacttcc ccgatcaaag cgtgtgccaa aaacaggcga actactcgac
     2641 tttaaccttc caaatcggcc attcgcacca ttaatcccgc ccgcacaaaa atgccgtctg
     2701 aaggcttcag acggcatttt ttgttcaaac atcaatacca accgcgcagt ttcatcgctt
     2761 tttcaacacg gcggatactc atcatataag acgcggttcg caaatcgaca tcgtattctt
     2821 gagccaagtt ccaaatatcg cggaacgctc ggcgcaggac gacggtttct ttctcttgaa
     2881 cttcgtcaaa ctcccaataa tagccttgca ggttttgcac ccactcgaaa taggaaacga
     2941 ccacgccgcc gcagttcgcc agaatatcag gcacgaccaa tacgccgttt tgacgcagga
     3001 taacgtcggc ttcgggcgta gtcgggccgt tcgcgccttc gaccacgatt ttcgcgcgga
     3061 ctttaccggc gttttcagaa gtcagttgat tttccagcgc gcaaggggcg agtacgtcca
     3121 catccaaagc caaaagttcg gcgttggtaa tttccttgcc gtaaccggct tcgttggtga
     3181 tgaagccttt ttcttggaac tctttgaaca gggcttccat atccaaaccg ttttcgttgt
     3241 aaatggcaac gtcaacagta gaaaccgcaa caactttcgc gccggattga tgcgcgtaat
     3301 aacctgtgtg gtaaccgaca ttaccgaagc cttggatggc gtaagtcgcg cctttcacgt
     3361 ccttgcccag tttttccaag gcttggacgg cggcgaggtt cacgccgtaa ccggtagcct
     3421 cggtacgcgc caaagagccg ccgaactcaa ccggtttacc ggtaaatacg cccggtgcag
     3481 aatgtttcac cacgttttca taggcatcaa ccatccaaga catgattttg ccgttggtgt
     3541 tcacatcggg ggcgggaata tcgatttttt cgccgatcag cggagaaatg gcttcggaat
     3601 aagcgcgggc gatgcgttcc agttccgcct cggaataatc gcgcggatcc aaggtaacgc
     3661 cgcctttgcc gccgccgtaa ggaatgcccg ctacgcagca tttgatggtc atccaaatcg
     3721 acagggcttt gacttcgtcc agattcactc cgggatggaa gcgcacgccg cctttatagg
     3781 ggccgacggc gttgttgtgt tgcgaacggt agcccgtgaa ggttttgacc gtgtcgtcgt
     3841 cgagtttgac ggggaaattg acttccaaca cgcgggtcgg actcttcagg atttcataaa
     3901 cggccggatc ggttttcagc cggtcgcagg cggttttgac ctgtttgcgc gcgatttcaa
     3961 acggattgag ggtttctttt gcaagggctt cagacatttt gcttcctttt cacaaaaaga
     4021 ttttcagggg cgtgcatttc gctcattact ataaccaatt tcccgacaaa atgtaatagc
     4081 gtgtagtcgg cataagcccg atttgattaa tctatatatg attttatttt tcaagccgca
     4141 cggaatcagc ccggactccg cctgaaaaag cagcgcgcgc gtccgaaaaa taaatgtcaa
     4201 attaaataaa gatataggca tccttttatt ttttaaaaat ttaattggaa catcgctggg
     4261 atttcaaacc cttgcccgat tccgccccga aatccgaaaa caccgcctgc aaaacctgtt
     4321 gcgattgtta acaatccata cattagaagc cctgtgcaaa cgatgttaaa ataaaccctt
     4381 tcaacccgac aggaaatcag attatgaatg cagccatcga acacgtccaa gccgtcgcct
     4441 tcgatttgga cggcacactg tgcgattccg tccccgacct tgccgccgcc gcagaagcga
     4501 tgttggaaca actcggtatg aaaccgctgc ccgccaaagt ggtcgaaagc tatgtgggcg
     4561 acggcatcgg caaactggtt caccgcgtcc tcaccaacga ccgcgaccgc gaagccgatt
     4621 ccgaactgtg ggaaaaaggt ttcgtatcct atatgaaata ctaccgcgac catttgagcg
     4681 tcttcacccg cccctatccc gaaaccgaag ccgggctggc attgctcaaa tctttgggca
     4741 tcccgctggc agtcattacc aacaaaaacg aaatccttgc cgtcgaactg ttgaaacagc
     4801 tcggacttgc cgactacttc agcctgatac tcggcggcga cagcctgccc gagaaaaaac
     4861 ccagccccct gccgctgcgg cacgccgccg aagttttggg tatcgatgtt gcaaacatgg
     4921 ttatggtcgg cgactcgcgc aacga
//
LOCUS       DAWHSK010000146         4926 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.146, whole genome shotgun sequence.
ACCESSION   DAWHSK010000146 DAWHSK010000000
VERSION     DAWHSK010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4926
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.146"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            46..450
                     /locus_tag="WIC03_001488"
     CDS             46..450
                     /locus_tag="WIC03_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEZ3883967.1"
                     /translation="MTAQPCPICTAQDEDILLQTPKLRVIAVHNDSGSPAFCRVIWRK
                     HIAEMTDLSAAERGELMEIVYKVEAAMRQVFRPAKINLASLGNVVPHLHWHIIARFEN
                     DASFPAPIWANPVRKHGMTLPQDWTEQLKKLL"
     gene            490..1674
                     /locus_tag="WIC03_001489"
     CDS             490..1674
                     /locus_tag="WIC03_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEZ3883968.1"
                     /translation="MTEPTSRRRFLKTCTAAAGAGLLQACGTSATSVPPLPSSHSVVK
                     ARTVPLQTPRRQSSDGNLLRVVASSGFAEDTNRVNTALTRLYNVGFTVTNQQAGSRRF
                     QRFAGTDTQRAADFQEVASGRVATPKVLMGLRGGYGAARILPHIDFASLGARMREHGT
                     LFFGFSDVCAVQLALLAKGNMMSFAGPMAYSDFGKPAPGAFTMDAFIKGATQNRLTVD
                     VPYIQRADVETEGILWGGNLSVLASLAGTPYMPDIDGGILFLEDVGEQPYRIERMLNT
                     LYLSGILKKQRAIVFGNFRMEKIRDVYDPSYDFSAVANHVSRTAKIPVLTGFPFGHIA
                     DKITFPLGAHARIRMNGNSGYSVAFGGYPTLDASALTLDTLLPPPDLPIFPESGVADI
                     SE"
     gene            complement(1737..2270)
                     /locus_tag="WIC03_001490"
     CDS             complement(1737..2270)
                     /locus_tag="WIC03_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEZ3883969.1"
                     /translation="MGIYDFQMKDAEGNAVDLSGYRGKVLLIVNTATRCGLTPQYEAL
                     QKLYAQYTAEGLEILDFPCNQFREQAPESSGEIAQVCMMKFGTKFKIFDKIEVNGANT
                     APLYAYLKSVKPQDKGNHLFKDFVLKLAALGEKRDEGDIKWNFTKFLVNRDGEVVERF
                     APSVTPEEIEADIRALL"
     gene            complement(2449..4704)
                     /locus_tag="WIC03_001491"
     CDS             complement(2449..4704)
                     /locus_tag="WIC03_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HEZ3883970.1"
                     /translation="MGQYKKLWYLLFAVLAVCFTILGYMGSEVYKKAPPYPEQVVSAS
                     GKVLMTKDDILAGQSAWQTTGGMEVGSILGHGAYQAPDWTADWLHRELVAWLDLTAQE
                     TYGKKFDEVSPEEQAVLKTRLADEYRNQSRVKEDGSVVVSETRVKAIESILPYYHGVY
                     SDDPKFQTTREHFAMKNNTLPSQEAREKLFNFFFWTSWSASTNRPDENFTYTNNWPHE
                     PLINNVPTTENYMWSFTSVVLLLMGIGLLMWGYSFLTKHEEVEVPSEDPISKIQLTPS
                     QKALGKYVFLTVALFVVQVLLGGLTAHYTVEGQGFYGIDEALGFEMSDWFPYALTRTW
                     HIQSAIFWIATGFLTAGLFLAPIVNGGKDPKFQRAGVNFLYIALFIVVGGSYAGNFFA
                     LTHILPPEFNFWFGHQGYEYLDLGRFWQLLLMVGLLLWLFLMLRCTVSAFKEKGVDKN
                     LLAIFVASMVGVGVFYAPGLFYGEKSPIAVMEYWRWWVVHLWVEGFFEVFATAAFAFV
                     FYNMGFVRRSTATASTLAAAAIFMLGGVPGTLHHLYFSGSTSASMAIGACFSALEVVP
                     LVLLGREAYEHWSYQHLSDWAKRLRWPLMCFVAVAFWNMIGAGVFGFLINPPISLFYI
                     QGLNTSAVHAHAALFGVYGFLALGFVLLVARYLKPNAQFDDKLMTWGFWLLNGGLVGM
                     IAISLLPVGAIQAYASITHGLWYARSEEFLQMEILDTLRWVRTAADLIFIGGAVCVAI
                     QATKIVFSRDK"
BASE COUNT         1254 a         1348 c         1205 g         1119 t
ORIGIN      
        1 gaaattatcc taccgttttt taaacacttt caggaataag gaaaaatgac cgcccaaccc
       61 tgccccatct gcacggcaca agatgaagat attttgctgc aaacccccaa gctgcgcgtc
      121 atcgccgtcc ataacgacag cggttcgcct gcattctgcc gcgtcatttg gcgtaagcat
      181 attgccgaaa tgaccgacct ttcggcagcg gaacgcggcg aattgatgga aatagtgtac
      241 aaagtcgaag ccgctatgcg ccaagtgttc cggccggcaa aaatcaacct cgccagcttg
      301 ggcaatgtcg tgccgcacct gcactggcat attatcgccc gctttgaaaa cgatgcgtcc
      361 ttccccgcgc cgatttgggc aaaccccgtc cggaaacacg gtatgaccct gccgcaagat
      421 tggacggaac agcttaaaaa gctgctttaa gcccgccgat gccgtctgaa accgtatgaa
      481 agggaaatta tgaccgaacc gacctcccgc cgccgttttc tgaaaacctg caccgccgcc
      541 gccggcgcgg gactgcttca ggcttgcggc acatccgcca catccgttcc gccccttccc
      601 tcttcccatt ctgttgtgaa agcccgaacc gtgcctctcc aaacgccacg ccgtcaaagt
      661 tcggacggca accttctgcg cgttgtcgct tcgtcaggat ttgccgaaga caccaaccgc
      721 gtcaacacag ccttaacccg cctttacaat gtcggtttta ccgtaaccaa ccaacaggcg
      781 ggcagccgcc gtttccaacg gtttgccggc acggacacgc aacgtgccgc cgatttccaa
      841 gaggtcgctt ccggccgcgt cgccacgcct aaagtgctga tgggcttgcg cggcggttac
      901 ggtgcggcgc ggattctgcc gcatatcgat tttgcttcgc tcggcgcaag gatgcgcgaa
      961 cacggcacgc tctttttcgg attcagcgac gtatgcgccg tccagctggc attgttggca
     1021 aaaggcaata tgatgagttt tgccggcccg atggcttata gcgattttgg caaacccgcc
     1081 cccggtgcgt ttacgatgga tgcctttatc aagggtgcaa cccaaaaccg cctgaccgtt
     1141 gatgttcctt atatccaacg cgccgatgtc gaaaccgaag gcatattgtg gggcggcaac
     1201 ttaagcgtcc tcgcctcgct cgccggcacg ccttatatgc ccgacatcga cggcggcatt
     1261 ttgttcctcg aagatgtcgg cgaacagccc taccgcatcg aacgtatgct caatacgctg
     1321 tatctttcgg gtattttgaa gaaacagcgc gccatcgtgt tcggcaattt ccgtatggaa
     1381 aaaattcgag atgtctatga tccgtcttat gatttttctg ccgttgccaa ccatgtttcg
     1441 cgcacggcga aaatccccgt gctgacgggc ttcccgttcg gacacattgc cgacaaaatc
     1501 actttccctc taggcgcgca cgcccgaatc cgtatgaacg gaaacagcgg ttattcggtc
     1561 gcgtttggag gctacccgac actcgatgcg tccgccctga ctttggatac cctgctccca
     1621 ccgccggatt tgcccatctt ccccgaaagc ggtgttgccg atatttcgga ataaacccgc
     1681 aaacggacaa atgccgtctg aagccttcag acggcatttc ccaagacggc ggcagattac
     1741 agcaatgccc gaatatcggc ttcgatttct tcgggcgtaa cactaggcgc aaaacgctcg
     1801 accacttcgc cgtcgcggtt gacgaggaat ttggtaaagt tccatttgat gtcgccttcg
     1861 tcgcgcttct ctcccaaagc tgcgagcttc aacacgaaat ctttaaacag atgattgcct
     1921 ttatcttgcg gtttgacgga tttcaggtag gcatacaagg gcgcggtatt tgctccattg
     1981 acttcgattt tgtcgaaaat cttaaacttc gtgccaaact tcatcataca cacttgggca
     2041 atttctccgc tgctttcggg agcctgttcg cggaactggt tgcacggaaa atccaaaatc
     2101 tccaagcctt ctgcggtata ttgtgcatac agcttctgca aagcctcgta ttgcggggtc
     2161 agaccgcaac gcgttgccgt gttgacaatc agcagaacct tgccgcgata gcctgacaaa
     2221 tcaaccgcat tgccttccgc atctttcatt tgaaaatcgt aaatacccat ttttatcctt
     2281 atctgatgta aaccgatgcc atctgaaacg tgcttcagac ggcatgaaag cagcaattgt
     2341 atagccgatt aaaataaaaa atccacatcc ttttccattc ccgtcccaat ccgcaataaa
     2401 aaactgcacc cgaaaacggg tgcagttgct catttcatac cgcaaaactt atttgtcgcg
     2461 gctgaatacg attttggtag cttggatagc aacgcaaaca gcaccaccga tgaagatcaa
     2521 gtcagcagca gtacgaaccc aacgcagagt atccaagatt tccatttgca ggaactcttc
     2581 gctacgcgca taccacaggc cgtgcgtgat agaggcgtat gcctgaatcg cgccgacagg
     2641 cagcaggctg atggcaatca taccgaccaa gccgccgttg agcagccaga aaccccaagt
     2701 catcaatttg tcgtcaaact gcgcgttcgg tttcaaataa cgggcaacca gcaatacgaa
     2761 gcccaatgcc aagaaaccgt acacaccgaa caaggcggcg tgcgcgtgaa cggcagaagt
     2821 gttcaaacct tggatataga acagggaaat cggcggattg atcaggaagc cgaatacgcc
     2881 ggcaccgatc atattccaaa aggcgactgc cacgaagcac atcagcggcc aacgcaggcg
     2941 tttcgcccag tcggacaggt gttggtaaga ccagtgttcg tatgcttcac ggcccagcaa
     3001 caccagcggc acgacttcca aagcggagaa gcaggcaccg attgccatag aggcggaggt
     3061 agagccggag aagtacaggt ggtgcagcgt acccggaacg ccgcccaaca taaagatggc
     3121 ggcagcggcc aaagtggagg cagtggcggt actgcggcgg acaaagccca tattgtagaa
     3181 gacaaatgcg aaggcggcag tggcaaatac ttcaaagaag ccttccaccc acaggtgaac
     3241 cacccaccaa cgccagtatt ccattacggc aatcggggat ttttcgccat agaacagtcc
     3301 cggtgcgtag aacacgccca caccgaccat agaagcgaca aagattgcca gcaggttttt
     3361 gtccacgcct ttttctttga aggcggaaac cgtgcagcgc aacatcagga acagccacaa
     3421 cagcagtccg accatcagca ggagttgcca gaaacgaccc aaatcgaggt attcgtaacc
     3481 ttggtgtccg aaccagaaat taaattcggg gggaaggatg tgcgtcaacg cgaagaagtt
     3541 gcccgcgtaa gaaccgccga ccacgatgaa gagggcgata tagaggaagt tcacaccggc
     3601 acgttggaac ttgggatctt tgccgccgtt gacaatcggc gcgaggaaca aacctgccgt
     3661 caaaaagccg gttgcaatcc agaagatagc ggattggatg tgccaagtac gggtcagggc
     3721 gtaggggaac cagtcggaca tttcaaagcc caacgcctcg tcgataccgt agaaaccctg
     3781 gccttcgacg gtgtagtgtg cggtcaggcc gcccagcaat acttgtacca caaacagggc
     3841 gaccgtcagg aagacgtatt tgcccaatgc tttttgcgaa ggggtcagtt ggattttgga
     3901 aatcgggtct tcagacggca cttccacttc ttcgtgtttg gtcaggaagg aataacccca
     3961 catcagcaaa ccgatgccca tcagcagaag aacaacgctg gtgaatgacc acatatagtt
     4021 ttcagtggtc ggtacgttgt tgatcaaagg ttcgtgcggc cagttgttgg tgtaagtaaa
     4081 attctcgtca ggacggttgg tcgaagcaga ccaagaagtc cagaagaaga agttgaacag
     4141 tttttcacgc gcttcttggc ttggcaatgt attgtttttc attgcaaagt gttcgcgagt
     4201 ggtttggaac ttaggatcgt cgctgtacac accgtggtag taaggcagga tgctttcgat
     4261 ggctttcacg cgggtttcgc tgacaacaac agaaccatct tccttaacac ggctttggtt
     4321 gcggtattcg tcagccaaac gtgttttcag aacagcttgt tcttcaggag aaacttcgtc
     4381 aaatttttta ccgtaagttt cttgcgcagt cagatccaac caggcaacca actcacgatg
     4441 cagccagtcc gccgtccagt ccggagcctg atatgcgccg tggcccagaa tcgaaccgac
     4501 ctccataccg ccggtagtct gccatgcaga ctgacctgcc aaaatatcgt ctttcgtcat
     4561 caagaccttg ccggatgcgg aaacgacctg ttcggggtaa ggcggggctt ttttgtaaac
     4621 ctcgctgccc atatagccaa gaatggtaaa gcataccgcc agaacggcaa acagcaagta
     4681 ccaaagcttc ttgtactgtc ccattttaag tactccttaa ttttggtaaa agtggttggg
     4741 tattaattca tcaaatatga atgttaaaga ttgtatcacg gtttaacaag taaagaaata
     4801 ttttgtcaaa aatattcctt taaaaccatt aacttatatg atattgattc ccattttctt
     4861 atacaaaccc tcctttatcg tatttgtgtt aaattttcgc taacattttc acacaaaatt
     4921 aaactt
//
LOCUS       DAWHSK010000147         4885 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.147, whole genome shotgun sequence.
ACCESSION   DAWHSK010000147 DAWHSK010000000
VERSION     DAWHSK010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4885
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.147"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(233..961)
                     /gene="rmpM"
                     /locus_tag="WIC03_001492"
     CDS             complement(233..961)
                     /gene="rmpM"
                     /locus_tag="WIC03_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding outer membrane protein
                     RmpM"
                     /protein_id="HEZ3883971.1"
                     /translation="MTKQLKLSALFVALLASGTAVAGEASVQGYTVSGQSNEIVRNNY
                     GECWKNAYFDKASQGRVECGDAVAAPEPEPEPEPAPAPVVVVEQAPQYVDETISLSAK
                     TLFGFDKDSLRAEAQDNLKVLAQRLGQTNIQSVRVEGHTDFMGSDKYNQALSERRAYV
                     VANNLVSNGVPVSRISAVGLGESQAQMTQVCEAEVAKLGAKVSKAKKREALIACIEPD
                     RRVDVKIRSIVTRQVVPAHNHHQH"
     gene            1325..2275
                     /locus_tag="WIC03_001493"
     CDS             1325..2275
                     /locus_tag="WIC03_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HEZ3883972.1"
                     /translation="MKLQQLKYALEVYRHNLNVSEAAEALFTSQPGISKQIKLLEEEI
                     GIQIFIRSGKRVVSVSQPGKVVLDIAERILRDVQNIKNIGSEFTGQDSGSLTVATTHT
                     QARYALPLIVADFVKRYPKVNLTIKQGSPAAIAQMVTSGESDLAIVTERIDDHPELGR
                     LSCYDWTHAVIVPNDHPLLECRNPLRIEDLARFPLITYEFAFNAGSSIARAFSKARLE
                     QPDVALAAADTDVLKTYVRLGLGVGLMAKMAYNPDTDGDLQLVDAAHLFEPSPTWIAL
                     RSDTYLRGYAYDFIQAFAPHLTREKVDRILYTPISEDFSI"
     gene            complement(2388..3122)
                     /gene="fnr"
                     /locus_tag="WIC03_001494"
     CDS             complement(2388..3122)
                     /gene="fnr"
                     /locus_tag="WIC03_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HEZ3883973.1"
                     /translation="MASHNTTHQMKTLCSSCSLRELCLPVGLLPNELSQLDAVIRQSR
                     RLKKGEYLFCVGEAFTSLFAIRSGFFKTTVASQDGRDQVTGFFMSGELIGMDGICSHV
                     HSCDAVALEDSEVCELPFTHIEELGQNIPSLRTHFFRMMSREIVRDQGVMLLLGNMRA
                     EERIAAFLLNLSQRLYSRGFAANDFILRMSREEIGSYLGLKLETVSRTLSKFHQEGLI
                     SVEHKHIKILNLQVLKKMVSGCSHAI"
     gene            3327..4748
                     /gene="hemN"
                     /locus_tag="WIC03_001495"
     CDS             3327..4748
                     /gene="hemN"
                     /locus_tag="WIC03_001495"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEZ3883974.1"
                     /translation="MKIIQIQNNHNVNDDRPEFDRALIASLPASGPRYTSYPTADRFH
                     DGFREGEYIKALHLRGMGALNKPLSLYIHIPFCNTICYYCGCNKIITKDKSRADAYIE
                     YLEKEMELLAPHLNGRHQLAQLHFGGGTPTFLSDEQIERVFRMIRKHFELIPTGEYSI
                     EIDPRKVSRDTVLMLGRLGFNRMSIGIQDFDPKVQAAVNRIQSYEETKEVIDAAREAG
                     FKSVSVDLIYGLPHQTSESIKTTIDTVLSLDPDRLALYHYAHLPHVFKPQRRIDTVAV
                     PDSEEKLDMLQYCVQTLTERGYVFIGMDHFAKPDDELSIALKEGFLQRNFQGYSTYAD
                     CDLVAIGVSSIGKIGSTYSQNERDIDAYYAAIDEGRLPIMRGYQLNQDDILRRNIIQD
                     LMCRFALDYRIYESMFGIPFDRYFKDELADLEKLAGLGLVRLNSHGLTVTPKGRFLIR
                     NIAMVFDYHLRHKETKAKYSQTV"
BASE COUNT         1241 a         1336 c         1183 g         1125 t
ORIGIN      
        1 cggcaatggg acacaaacct gaatctgaat ctgaatctga atccgaatcc gaatccgaac
       61 ctgcaccccg attctgacga tagccgtttc agaaaaccac ccaaaaaaac aaaccccgca
      121 aaagcggggg tttgtttcac atcatcgatg ccgaccgacg tttcacgaat attgcaacag
      181 tacccggtac aaaatccgct aacctcatgc atcggcaaga tattgcctag ccttagtgtt
      241 ggtgatgatt gtgtgccggc acaacctgac gggttacgat gctgcggatt ttcacatcca
      301 cacggcggtc aggttcgata catgcaatca gagcctcacg ttttttggct ttagagactt
      361 tcgcacccag tttggcaact tcggcttcac aaacttgagt catttgcgct tgagattcgc
      421 ccaagccaac agcagaaatt ctagaaacag gtacgccgtt gctgaccagg ttgtttgcca
      481 ctacgtatgc gcggcgttcg gacagggcct gattgtattt gtcagaaccc ataaagtcgg
      541 tatggccttc gacgcggaca gattggatat tggtttgacc caggcgttgc gccaatactt
      601 tcaggttgtc ttgagcttcg gcgcgcaatg aatccttatc gaaaccgaac agggttttgg
      661 cagacaggga aatggtttca tcaacatatt gcggagcctg ctccacaacg acgacaggcg
      721 cgggtgcggg ttcgggttct ggctcgggtt cgggggcagc aaccgcatcg ccgcattcta
      781 cgcgaccttg gcttgcttta tcaaagtagg cgtttttcca gcattcgcca tagttgttgc
      841 gtacgatttc gttcgactgg ccgcttacgg tgtaaccctg aacggacgcc tcgcccgcaa
      901 cagcagtgcc ggaagcgagc aatgcaacga ataatgcgct taatttcagc tgtttggtca
      961 ttttattccc tcattaaatt tgtacagcag atacagaagc aactgctgaa ttgccaacat
     1021 acaggccgct gtagaaacat ggcggcaaat cggatatttc agtctccact ataaagaagc
     1081 agggggagga ctgtcaatac tcaacgcctg aaaacaaccg taaaacagcc tgtttcctga
     1141 tagtctatca tgccgaaaaa tcaattccgt gtcattactt tagggggatt ttttccatat
     1201 cgcacgattg ccgttgcaca aaaacaacaa acacaacagc aaagccccat accgtaccgg
     1261 caagaaaata tgataaatta taaacaatgt tcagccaccc gacacagacc cacaccgacc
     1321 cgccatgaaa ttacaacaat tgaaatacgc cttagaagtt taccggcaca acctgaacgt
     1381 ttccgaagcg gccgaagcct tgtttacttc gcaacccggc atctccaaac aaatcaaatt
     1441 gctggaagaa gaaatcggca ttcagatttt tatccgcagc ggcaagcgcg tggtttcggt
     1501 ctcgcagccg ggcaaggtgg ttttggatat tgcagaacgt attttgcgcg atgttcagaa
     1561 cattaaaaat atcggcagcg agtttaccgg acaggacagc ggttcgctga cggttgccac
     1621 gacgcatact caggcacgct atgccctacc cttgattgtt gccgattttg tgaaacgcta
     1681 tccgaaagtc aacctgacca tcaaacaggg gagccctgcc gccatcgccc aaatggttac
     1741 ttcgggagag tctgatttgg caattgttac ggaacgtata gacgaccacc ccgaactggg
     1801 aagactttcc tgctacgact ggacacacgc ggttatcgta ccgaacgacc accccttgct
     1861 cgaatgcaga aaccccctcc gtattgaaga tttggcgagg tttccgctga ttacttatga
     1921 atttgcattc aatgcgggca gcagcatcgc gcgggcattt tccaaagccc gtttggaaca
     1981 gccggatgtc gcattggcgg cggcggatac ggacgtattg aagacttatg tgcgcttggg
     2041 tttgggcgtg ggactgatgg cgaaaatggc gtacaacccg gatacggacg gcgatttgca
     2101 gcttgtggat gcggcacacc tgttcgagcc gtcgccgacg tggattgctt tgcgcagcga
     2161 tacttatttg cgcggatatg cctacgactt tatccaagcg tttgcgccgc acctgacacg
     2221 cgagaaggtg gataggattc tgtacacgcc catcagcgag gatttttcga tttaggcggc
     2281 tgccggtttt cagacggcac tttgcggcag atacaacaaa caggacagat gttttcgtct
     2341 gccctgtgtt tattgagaat gccgtctgaa atgttcgtac gggttaatca aatggcgtgc
     2401 gagcagccgg acaccatttt tttcaacacc tgcagattga ggattttgat gtgcttatgc
     2461 tcgacggaaa tcaatccttc ctgatgaaat ttagataatg tgcggctgac ggtttcaagt
     2521 ttcagcccga gataactgcc gatttcttcg cgggacattc ttaagatgaa gtcgttggca
     2581 gcaaaacctc gggaataaag gcgttgggaa aggttcagca ggaaggcggc aatccgctct
     2641 tcggcgcgca tattgcccaa cagcagcata acaccttggt cgcgcacgat ttcacggctc
     2701 atcatgcgga agaagtgcgt acgcaggctg gggatgtttt gccccagttc ttcgatgtgg
     2761 gtaaacggca gttcgcacac ttcgctgtct tccaaggcga ccgcgtcgca actgtgcaca
     2821 tgggaacaga tgccgtccat gccgatgagt tcgcccgaca taaagaaacc cgttacctga
     2881 tcgcggccgt cctgactggc gacggttgtt ttgaagaagc ccgaacggat ggcaaagagc
     2941 gaggtaaagg cttcgccgac acagaacagg tattcgccct ttttcaggcg gcggctttga
     3001 cggatgacgg catcgagttg gctgagctcg ttgggcagca gcccgacagg caggcagagt
     3061 tcccgcaaag aacaggaaga acacagcgtt ttcatctgat gtgtagtatt atgcgaagcc
     3121 ataccgtacc tttttgtgcg ctttgcccca tcatgattat agtggattaa atttaaacca
     3181 gtacggcgtt gcctcgcctt gtccttattt aaatttaatc cactatatgt gcttattgac
     3241 acatatcaag acaggtttat catactgtgg cattctacca aactatccaa aatttgacta
     3301 acatcataaa accgcacaca cacaccatga aaatcattca gatacagaac aatcacaatg
     3361 taaacgatga ccgccccgag tttgaccgcg cgctgattgc cagcctgccc gccagcggcc
     3421 cgcgctacac ttcctaccct accgccgacc gtttccatga cggtttccgc gaaggcgaat
     3481 atatcaaagc cttacatttg cgcggtatgg gcgcgttaaa caaaccgctt tccctttaca
     3541 ttcacattcc gttctgcaac accatctgct actactgcgg ctgcaacaaa atcatcacca
     3601 aagacaaaag ccgcgccgat gcctacatcg aatatcttga aaaagaaatg gaactgctcg
     3661 ctccacatct gaacggacgg caccagcttg cccaactgca cttcggcggc ggcacgccga
     3721 cctttttgag cgacgaacag atcgaacgtg tcttccgcat gatacgcaaa catttcgagt
     3781 taatccccac cggcgaatac tccatcgaaa tcgacccgcg caaagtcagc cgcgacaccg
     3841 tcctcatgct cggcaggctc ggcttcaacc gcatgagcat cggcattcag gatttcgacc
     3901 ccaaagtgca ggcggcggtc aaccgcatcc aaagttacga agaaaccaaa gaagtcatcg
     3961 atgcggcgcg cgaagcgggg ttcaaatccg tcagcgtcga tttgatttac ggcctgccgc
     4021 accagacttc ggaaagcatc aaaaccacca tcgataccgt tttgtcgctc gatcccgacc
     4081 gcctcgccct ttatcactac gcccacctgc cgcacgtgtt caaaccgcaa cgccgcatcg
     4141 ataccgtcgc cgttcccgac agcgaagaga agctcgatat gctgcaatac tgcgtccaaa
     4201 ccctaaccga acgcggctac gtcttcatcg gcatggacca tttcgccaaa cctgacgacg
     4261 aactctccat cgccctcaaa gaaggcttcc tccagcgcaa cttccaaggc tattcgacct
     4321 acgcggattg cgatttggtc gccatcggcg tgtcgtccat cggcaaaatc ggcagcacct
     4381 attcccaaaa cgaacgcgac atcgatgcct actatgccgc catcgacgaa ggcagactgc
     4441 ccatcatgcg cggttatcag ctcaatcagg acgacatcct gcgccgcaac atcattcagg
     4501 atttgatgtg ccgtttcgcg ctcgactatc ggatttacga aagtatgttc ggcatcccgt
     4561 tcgaccgcta cttcaaagac gaactggcgg atttggaaaa actcgccggt ttgggattgg
     4621 tgcgcctgaa cagccacggg ctgaccgtta ccccgaaagg acgcttcctc atccgcaaca
     4681 tcgccatggt cttcgattac cacctgcgcc ataaagaaac caaggcgaaa tattcgcaaa
     4741 cggtgtgatt gcgtctaacg tacaaatgcc gtctgaaagg ctttttcaga cggcattttg
     4801 ctgccggcag gataagtgtt ttcaagaaca ggcggcggca tatcataacg ttccgcacct
     4861 ttgtgtccga ccgttccgaa accaa
//
LOCUS       DAWHSK010000148         4868 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.148, whole genome shotgun sequence.
ACCESSION   DAWHSK010000148 DAWHSK010000000
VERSION     DAWHSK010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4868
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.148"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..144
                     /locus_tag="WIC03_001496"
     CDS             <1..144
                     /locus_tag="WIC03_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HEZ3883975.1"
                     /translation="NVGIGSGALLGHWVTQYVGISRIGIAGMLAAAAGLWLCLNLNRH
                     IQT"
     gene            274..1710
                     /locus_tag="WIC03_001497"
     CDS             274..1710
                     /locus_tag="WIC03_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HEZ3883976.1"
                     /translation="MPLLSVEFALFFLAFLPIYWGLAKYPSVQNLLLLAAGMGWLYHI
                     GPVFAAIVVLYSSCVYLLGELLRSDRENTRRFWLGCGIAVSLTVLGFFKYFDFFRPLI
                     AQYAGQGGAIDILMPLGLSYYTFQSIAYLVYCFRAPHAARFEWHELLLHLSFFPTVTS
                     GPIIRAAAFKSADGEQAGALAQIRTHQPRSPVRPALAVSLILLGIAKKWWLAGMLAEN
                     WVSPVFENPAQFDGWGVLAGVYGYTFQLFLDFSGYSDLVIGMAMLLGFRLPKNFSAPL
                     RAANIRAFWDKWHISLSTWIRDYIYIPLGGSKKGFLRTQFNLMAAMVLSGIWHGYGWN
                     FLIWGALHGTALVLLNTGDRYFGRNALCRLKYLAPLSWLITFHFVCLTFVVFNTANPD
                     DAGAVFNALFANANGWNAPQQADMLLLASFASVMLLYPYLQRAFDGAVKGLEKIPMWL
                     WFIPVSAVLLLIIVLAPSGIPGFIYANF"
     gene            1721..2707
                     /locus_tag="WIC03_001498"
     CDS             1721..2707
                     /locus_tag="WIC03_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF459 domain-containing protein"
                     /protein_id="HEZ3883977.1"
                     /translation="MKNFLSLFASILMSALIAVWFSQNPINAYWQQTYHRNSPLEPLA
                     AYGWWRSGAALQENAYTLSDGIKAFLSGETPPTAQDGGSADMPPEAAASEAVPQTGEI
                     QEWKQNTDTAAVRSGDKVFFAGDSLMQGVAPFVQKSLKQQYGIESVNLSKQSTGLSYP
                     SFFDWPKTIEETLQKHPEISVLAVFLGPNDPWDFPVGKHYLKFAADEWAQEYLKRTDR
                     ILEAARAHHVQVVWLGIPYMKKAKLDGQMRYLDKLLSEHLEGKIILIPTAHTLSGGED
                     RYTDSVNVNGKPVRYRSKDGIHFTAEGQKLLAAKIMEKIVFEPSTQPSSTQP"
     gene            2704..3897
                     /locus_tag="WIC03_001499"
     CDS             2704..3897
                     /locus_tag="WIC03_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEZ3883978.1"
                     /translation="MNPKHLIAFSALFAATQAEALPVASVSLDTVTVSPSAPYTDTNG
                     LLTDYGNAAASPWMKKLRSVAQGSGETFRILQIGDSHTAGDFFTDTLRKRLQKTWGDG
                     GIGWVYPANVKGQRMAAVRHNGNWQSLTSRNNSGDFPLGGILAHTGSGGSMTLTASDG
                     KTGKQRISLFAKPLLAEQTLTVNGNTVSANGGGWQVLDTRAALPLTIQTEMPWDIGFI
                     NIENPAGGITVSAMGINGAQLTHWSKWRADRMNDLAQTGADLVILSYGTNEAFGDNID
                     IADTEQKWLDTVRQIRDSLPAAGILIIGAPESLKNTLGVCGTRPVRLTEVQQMQRRVA
                     RQGQTMFWSWQNAMGGICSMKNWLNQGWAAKDGVHFSAKGYRRAAEMLADSLEELVRA
                     AAIRQ"
     gene            4146..>4868
                     /locus_tag="WIC03_001500"
     CDS             4146..>4868
                     /locus_tag="WIC03_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEZ3883979.1"
                     /translation="MNRTYANFYEMLAAACRKNGNGTAVFDGKEKTAYRALKQEAEAV
                     AAYLQNIGVKFGDTVALAVSNSTEFITAYFAVSAIGAVAVPMNTFLKNSEYAYILNDC
                     KARFLFASAGLSKELAGLKAQTPVEKIIWTDKSRPAGEMAEGDAFFEDVRRFPEKPDL
                     GRQPRINDLAHIIYTSGTTGHPKGALISYANLFANLNGIERIFKISKRDRFIVFLPMF
                     HSFTLTAMVLLPIYMACSIILVK"
BASE COUNT         1074 a         1568 c         1226 g         1000 t
ORIGIN      
        1 aacgtcggta tcggcagcgg cgcactgttg ggacattggg taacgcaata tgtcggcatt
       61 tcccgtatcg gcatcgccgg tatgcttgcg gcggcggccg ggctgtggct gtgcctgaac
      121 ttgaaccgcc atatccaaac ctgaccgcgc gatgccgtct gaagcccctc ccgcccttca
      181 gacggcattt tgattgaaga aatatccgcc cctgcttaaa ataacgggct ttgccgtgtt
      241 ttaacgccta ttttcttatt ccaaggatgg tttatgccgc tgctgtctgt cgagttcgca
      301 ctgttctttc tcgccttcct gccgatttac tggggcttgg cgaaataccc gtccgtccaa
      361 aacctgctgc ttttggctgc cggtatgggc tggctctacc atatcggccc cgtatttgcg
      421 gcaatcgtcg tcctttattc ctcctgcgtg taccttttgg gcgaactgct ccgttccgat
      481 cgcgaaaata cgcgccgctt ttggctgggg tgcggcattg ccgtctcgct gaccgtcttg
      541 ggctttttca aatatttcga ctttttccgc ccgctgattg cacaatatgc cggacaaggc
      601 ggcgcaatcg acatcctgat gccgctgggg ctttcgtatt acaccttcca gtcaatcgcc
      661 tatttggttt actgcttccg tgcgccgcac gccgcgcgtt tcgagtggca cgaactgctg
      721 ctgcacctga gttttttccc caccgttacc tccggcccga ttatccgcgc cgccgcattc
      781 aaaagcgcgg acggcgagca ggcgggcgca ttggcgcaaa tccgtaccca ccaaccacgc
      841 tcacccgtcc gccccgcact cgccgtttcc ctgattctgc tgggtattgc caaaaaatgg
      901 tggctggcgg ggatgctcgc agaaaactgg gtatcgcccg tatttgaaaa tcccgcccaa
      961 ttcgacggct ggggcgtatt ggcgggcgtg tacggctata ccttccaact ctttttagac
     1021 ttttccggat attccgactt ggttatcgga atggcaatgc tgctgggctt ccggctgccc
     1081 aaaaacttct ccgcaccgct tcgtgccgcg aatatccgcg cattttggga caaatggcac
     1141 atcagccttt ccacctggat acgcgactac atctacatcc ccttgggcgg cagcaaaaaa
     1201 ggctttttgc ggacacagtt caacctgatg gcggcaatgg tgctctcagg catctggcac
     1261 ggctacggct ggaacttcct catttggggt gcactacacg gcacggcgct ggtgctgctc
     1321 aacacgggcg accgctattt cggacgcaac gcactatgcc gtctgaaata ccttgcgccg
     1381 ctctcgtggc tcatcacctt ccattttgtc tgcctgacct tcgtcgtctt caataccgca
     1441 aaccccgacg atgcaggcgc agttttcaat gccctctttg ccaatgccaa cggctggaat
     1501 gcgccgcaac aggcggatat gctgctgctt gcctcgtttg catccgtgat gctgctctac
     1561 ccttacctgc aacgcgcttt cgacggcgcg gtcaaaggtt tggaaaaaat cccgatgtgg
     1621 ctgtggttta tccccgtttc cgccgtcctg ctgctgatta tcgtattggc cccctcgggg
     1681 atacccggct ttatttatgc caatttttaa gggtttggac atgaaaaact ttctttccct
     1741 tttcgcctcc atattgatgt ctgccctgat tgccgtgtgg ttcagccaaa accccatcaa
     1801 cgcctactgg cagcagacct accaccgcaa cagcccgctc gaaccgcttg ccgcctacgg
     1861 atggtggcgg agcggtgcgg cattgcaaga aaacgcctac accctttcag acggcatcaa
     1921 agccttcctg tccggcgaaa cgccgccgac ggctcaagac ggcggttcgg cagatatgcc
     1981 gcctgaagcc gccgcatccg aagccgtccc tcaaaccggt gaaatccaag aatggaaaca
     2041 gaacaccgac accgccgccg tccgtagcgg cgacaaagtc ttttttgccg gcgactcgct
     2101 gatgcagggc gttgccccct tcgtgcaaaa aagcctgaaa caacaatacg gcatcgaatc
     2161 cgtcaacctc agcaaacaaa gcacagggct gtcctacccc tcattcttcg actggccgaa
     2221 aacgattgaa gaaaccctgc aaaaacatcc cgaaatcagc gtgctcgccg tcttcctcgg
     2281 cccgaacgac ccgtgggatt tccccgtcgg caaacactat ctcaaattcg ctgccgacga
     2341 atgggcgcaa gaatacctga aacgcaccga ccgcatcctt gaagccgccc gcgcgcacca
     2401 cgtccaagtc gtctggctcg gcatccccta catgaaaaaa gccaagctcg acgggcagat
     2461 gcgctacctc gacaaactgc tttcggaaca cttggaaggc aaaatcatcc tgattcccac
     2521 cgcgcacacc ctgagcggcg gggaagaccg ctacaccgat tccgtcaacg tcaacggcaa
     2581 acccgtccgc taccgcagca aggacggcat acactttacc gccgaaggac aaaaactgct
     2641 ggcggcaaaa ataatggaaa aaatcgtttt tgaaccaagt acgcaaccat caagtacaca
     2701 gccatgaacc ccaaacacct catcgcattt tccgccctgt tcgccgccac gcaggcagaa
     2761 gccctgcctg tcgcctccgt cagcctcgac accgttaccg tttccccgtc cgccccctac
     2821 accgatacaa acgggctgct gaccgactac ggcaacgccg ccgcctcgcc ttggatgaaa
     2881 aaactccgat ccgtcgcaca aggcagcggc gagaccttcc gcatcctgca aatcggcgac
     2941 tcacataccg ccggcgactt ctttaccgac accctgcgca aacgcctgca aaaaacttgg
     3001 ggcgacggcg gcataggctg ggtttacccc gccaacgtca aagggcagcg catggcggcc
     3061 gtccggcaca acggcaactg gcaaagcctc accagcagga acaatagcgg agacttcccg
     3121 ctcggcggca tcctcgccca caccggcagc ggcggcagca tgaccctgac cgcatcggac
     3181 ggcaagaccg gcaaacagcg catttccctg tttgccaaac ccctgctcgc cgaacaaacc
     3241 ctgaccgtca acggcaacac cgtctccgcc aacggcggcg gctggcaggt actggatacc
     3301 cgcgcggcac tgcccttgac catacagacc gaaatgccgt gggacatcgg cttcatcaac
     3361 atcgaaaatc ccgccggcgg cattaccgtt tccgccatgg gcatcaacgg cgcacaattg
     3421 acccattggt cgaaatggcg tgccgaccgt atgaacgacc ttgcccaaac cggcgccgat
     3481 ttggttatcc tttcctacgg caccaacgaa gccttcggcg acaacatcga cattgccgac
     3541 accgaacaaa aatggctgga taccgtccgc caaatccgcg acagcctgcc tgccgccggc
     3601 atcctcatca tcggcgcacc cgaatccctg aaaaacacgc tcggcgtatg cggcacacgc
     3661 cccgtccgcc tgaccgaagt ccaacagatg cagcggcgcg tcgcccgaca gggacaaacc
     3721 atgttctggt cttggcaaaa cgccatgggc ggcatatgca gcatgaaaaa ctggctcaac
     3781 caaggatggg ccgccaaaga cggcgtacac ttctccgcca aaggctaccg gcgcgcggcg
     3841 gaaatgctcg ccgacagcct cgaagaactc gtccgcgccg ccgcaatcag gcaataatcg
     3901 gacaggaggc ggacggtatt tccgcaacag ggggatgccg tctgaaacgc ataccttcat
     3961 attgcttcag acggcattcg cctcagacat tggaaagacg caggcgaacc tccggcatac
     4021 ggcgcaaagg cggcgtttga tatgccgtct gaaggcaaag atgataaact gccgccttcc
     4081 gttttcatac ggcatattgt tttcaaatga gggcgttcgc cgtccgcaac cataaaggaa
     4141 gtttcatgaa ccggacttat gccaatttct acgaaatgct cgccgccgcc tgccgcaaaa
     4201 acggcaacgg cacggcagtg ttcgacggca aggaaaaaac cgcctaccgc gcgctcaagc
     4261 aggaggccga agccgtcgcg gcgtatctgc aaaatatcgg cgtgaagttc ggcgacacgg
     4321 tcgcgctggc ggtttccaat tccacagaat ttattaccgc ctatttcgcc gtctccgcca
     4381 tcggcgcggt cgccgtaccg atgaacacat ttttgaaaaa cagcgaatac gcgtatatcc
     4441 tgaacgactg caaggcgcgc ttcctgttcg cctcggcggg cctgtcaaaa gagttggcgg
     4501 gcttgaaggc gcaaacgccc gtcgaaaaaa tcatttggac ggacaaaagc cgtccggccg
     4561 gcgaaatggc ggaaggcgat gccttttttg aagacgtgcg ccgcttcccc gaaaaacccg
     4621 acttgggccg ccaaccccgg ataaatgatt tggcacacat catctacacc tccggcacga
     4681 cggggcatcc caaaggcgcg ctaatcagtt acgccaacct gttcgccaac ctgaacggca
     4741 tcgaacgcat ctttaaaatc tccaagcgcg accgctttat cgttttcctg ccgatgttcc
     4801 acagcttcac gctgacggct atggtgctgc tgccgattta tatggcgtgt tcgattattt
     4861 tggtcaaa
//
LOCUS       DAWHSK010000149         4866 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.149, whole genome shotgun sequence.
ACCESSION   DAWHSK010000149 DAWHSK010000000
VERSION     DAWHSK010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4866
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.149"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..445
                     /locus_tag="WIC03_001501"
     CDS             <1..445
                     /locus_tag="WIC03_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEZ3883980.1"
                     /translation="SARAGYFFTPENKKLGTFIIRGQAGYTLARDNADVPSGLMFRSG
                     GASSVRGYELDSIGLAGPNGSVLPERALLVGSLEYQLPFTRTLSGAVFHDMGDAAANF
                     KRMKLKHGSGLGVRWFSPLAPFSFDIAYGHSDKKIRWHISLGTRF"
     gene            500..4669
                     /locus_tag="WIC03_001502"
     CDS             500..4669
                     /locus_tag="WIC03_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB"
                     /protein_id="HEZ3883981.1"
                     /translation="MTDTAPTDTDPTENGTRKMPSEHRPTPPAKKRRPLLKLSAALLS
                     VLILAVCFLSWLAGTESGLRFGLYQIPSWFGVNISSQNLKGTLLDGFDGDNWSIETEG
                     ADLKISRFRFAWKPSELMRRSLHITEISAGDIAIVTKPTPPKEERPPLSLPDSIDLPA
                     AVYLDRFETGKISAGKAFDKPTVYLERLDASYRYDIKGHRLDLKAADTPWSNSSGSAS
                     VGLKKPFALDTAIYTKGGLEGKTIHSTARLSGSLKDVRAELAIDGGNIRLSGKSVIHP
                     FAESLDKTLEEVLVKGFNINPSAFVPSLPDAGLNFDLTAIPSFSDGIALEGSLDLENT
                     KAGFADRNGIPVRQVLGNFVIRQDGTVHIGNTSAALLGRGGIRLSGKIDTEKDILDLN
                     IGINSVGAEDVLQTAFKGRLDGSIGIGGTTASPKISWQLGIGTARTDGSLAIASDPAN
                     GQRKLVLDTVNIAAGQGSLTAQGYLELFKDRLLKLDIRSRAFDPSRIDPQLPAGNING
                     SINLAGELAKEKFTGKMRFLPGTFNGVPIAGSADIVYESRHLPRAAVDLRLGRNIIKT
                     DGGFGKKGDRLNLNITAPDLSRFGFGLAGSLNVRGHLSGDLDGGIRTFETDLSGAARN
                     LHIGKAADIRSLDFTLKGSPDTSRPIRADIKGSRLSLSGGAAVVDTADLMLDGTGVQH
                     RIRTHAAMTLDGKPFKFDLDASGGINRELTRWKGSIGILDIGGAFNLKLQNRMTLEAG
                     AERVAASAANWQAMGGSLNLQHFSWDKKTGISAKGGAHGLHIAELHNFFKPPFEHNLV
                     LNGDWDVAYGRNARGYLNISRQSGDAVLPGGQALGLNAFSLKTRFQNDRIGILLDGGA
                     RFGRINADLDIGNAFGGNMANAPLGGRITASLPDLGTLKPFLPAAAQNITGSLNAAAQ
                     IGGRVGSPSVNAAVNVSSNYGKINGNITVGQSNSFSTSPLGGKLNLTVADAEVFRNFL
                     PVGQTVKGSLNAAVTLGGSIADPHLGGSINGDKLYYRNQTQGIILDNGSLRSHIAGRK
                     WVIDSLKFRHEGTAELSGTVGIENSGPDVDIGAVFDKYRILSRPNRRLTVSGNTRLRY
                     SPQKGISVTGMIKTDQGLFGSQKSSMPSVGDDVVVLGEVKKEAAAPLPVNMNLTLDLN
                     DGIRFAGYGADVTIGGKLTLTAQSGGSVRGVGTVRVIKGRYKAYGQDLDITKGTVSFV
                     GPLNDPNLNIRAERRLSPVGAGVEILGSLNSPRITLTANEPMSEKDKLSWLILNRAGS
                     GSSGDNAALSAAAGALLAGQINDRIGLVDDLGFTSKRSRNAQTGELNPAEQVLTVGKQ
                     LTGKLYIGYEYSISSAEQSVKLIYRLTRAIQAVARIGSRSSGGELTYTIRFDRFSGSD
                     KKDSAGNSKGK"
BASE COUNT         1121 a         1553 c         1308 g          884 t
ORIGIN      
        1 ctctgcccgc gcaggttatt tcttcacgcc cgaaaacaag aaactcggca cgttcatcat
       61 acgcggacaa gcgggttaca cccttgcccg cgacaatgcc gatgtcccct cggggctgat
      121 gttccgcagc ggtggcgcgt cttccgtgcg cggttacgaa ctcgacagca tcggacttgc
      181 cggcccgaac ggatcggtcc tgcccgaacg cgccctcttg gtgggcagcc tggaatacca
      241 actgccgttt acgcgcaccc tttccggcgc ggtattccac gatatgggcg atgccgccgc
      301 caatttcaaa cgtatgaagc tgaaacacgg ttcgggactg ggcgtgcgct ggttcagccc
      361 gcttgcgccg ttttccttcg acatcgccta cgggcacagc gataagaaaa tccgctggca
      421 catcagcttg ggaacacgct tctaaaccga tacggccgct tcagacggca ttacagcaaa
      481 ccattttgaa acagacatta tgaccgatac cgcaccgaca gataccgatc cgaccgaaaa
      541 cggcacgcgc aaaatgccgt ctgaacaccg ccccaccccg ccggcaaaaa aacgccgccc
      601 gctgctgaag ctgtcggcgg cactgctgtc tgtcctgatt ttggcagtat gtttcctcag
      661 ctggctcgcc ggtacggaat ccggtttgcg tttcgggctg taccaaatcc cgtcttggtt
      721 cggcgtaaac atttcctccc aaaacctcaa aggcacgctg ctcgacggct tcgacggcga
      781 caactggtcg atagaaaccg agggggcaga ccttaaaatc agccgcttcc gcttcgcgtg
      841 gaaaccgtcc gaactgatgc gccgcagcct gcacatcacc gaaatttccg ccggcgacat
      901 cgccatcgtt accaaaccga ctccgcctaa agaagaacgc cctccgctca gccttcccga
      961 cagcatagac ctgcctgccg ccgtctatct cgaccgcttc gagacgggca aaatcagcgc
     1021 aggcaaagcc tttgacaaac caaccgtcta tctcgaacgg ctggatgctt cataccgtta
     1081 tgacatcaaa ggacaccgcc tcgacctgaa ggctgccgac acgccgtgga gcaattcgtc
     1141 ggggtcagcc tcggtcggct tgaaaaaacc gtttgccctc gataccgcca tttacaccaa
     1201 aggcggactc gaaggcaaaa ccatacacag tacggctcgg ctgagcggca gcctgaagga
     1261 tgtgcgcgcc gaactggcga tcgacggcgg caatatccgc ctctcgggaa aatccgtcat
     1321 ccacccgttt gccgaatcat tggataaaac attggaagaa gtactggtca aagggttcaa
     1381 catcaatccg tccgccttcg tgccttccct gcccgatgcc gggctgaatt tcgacctgac
     1441 cgccatcccg tcgttttcag acggcatcgc gctggaaggc tcgctcgatt tggaaaacac
     1501 taaagccggt tttgccgacc gcaacggcat ccccgtccgt caggttttag gcaactttgt
     1561 catccggcag gacggcacgg tgcatatcgg caatacgtcc gccgccctgc tcggacgggg
     1621 cggcatcagg ctgtcgggca aaatcgacac cgaaaaagac atcctcgatt taaatatagg
     1681 catcaactcc gtcggcgcgg aagacgtgct gcaaaccgcg ttcaaaggca ggttggacgg
     1741 cagcatcggc atcggtggca cgaccgcctc gcccaaaatc tcttggcaac tcggcatcgg
     1801 cacagcgcgc acggacggca gcctcgccat tgcaagcgac cccgcaaacg gacagcggaa
     1861 actggtgctc gacaccgtca acatcgccgc cgggcaaggc agcctgaccg cgcaaggcta
     1921 tctcgagctg tttaaagacc gcctgctcaa gctggacatc cgttcccgcg cattcgaccc
     1981 ttcgcgcatc gatccgcaac ttccggcagg caatatcaac ggctcaataa accttgccgg
     2041 cgaactggca aaagagaaat tcacaggcaa aatgcggttt ttacccggca cgttcaacgg
     2101 cgtaccgatt gccggcagtg ccgacattgt ttacgagtcc cgccaccttc cgcgtgccgc
     2161 cgtcgatttg cggctggggc ggaacattat taaaacagac ggcggcttcg gcaaaaaagg
     2221 cgaccggctt aacctcaata tcaccgcacc cgatttatcc cgtttcggtt tcggactcgc
     2281 ggggtcttta aatgtacgcg gacacctttc cggtgatttg gacggcggca tccgaacctt
     2341 tgaaaccgac ctttccggcg cggcgcgcaa cctgcacatc ggcaaggcgg cagacatccg
     2401 ttcgctcgat ttcacgctca aaggttcgcc cgacacaagc cgcccgatac gcgccgacat
     2461 caaaggcagc cgcctttcgc tgtcgggcgg agcggcggtt gtcgataccg ccgacctgat
     2521 gctggacggc acgggcgtgc agcaccgcat ccgcacacac gccgccatga cgctggatgg
     2581 caaaccgttc aaattcgatt tggacgcttc aggcggcatc aacagggaac ttacccgatg
     2641 gaaaggcagc atcggcatcc tcgacatcgg cggcgcattc aacctcaagc tgcaaaaccg
     2701 tatgacgctc gaagccggtg cggaacgcgt ggcggcaagt gcggcaaatt ggcaggcaat
     2761 gggcggcagc ctcaacctgc aacacttttc ttgggataaa aaaaccggca tatcggcaaa
     2821 aggcggcgca cacggtctgc atatcgccga gttgcacaat ttcttcaaac cgcccttcga
     2881 acacaatctg gttttaaacg gcgactggga tgttgcctac gggcgcaacg cgcgcggcta
     2941 cctcaatatc agccggcaaa gcggcgatgc cgtattgccc ggcgggcagg ctttgggttt
     3001 gaacgcattt tccctgaaaa cgcgctttca aaacgaccgt atcggaatcc tgcttgacgg
     3061 cggcgcgcgt ttcgggcgga ttaacgccga tttggacatc ggcaacgcct tcggcggcaa
     3121 tatggcaaat gcaccgctcg gcggcaggat taccgcctcc cttcccgact tgggcacatt
     3181 gaagcccttt ctgcccgccg ccgcgcaaaa cattaccggc agcctgaatg ccgccgcgca
     3241 aatcggcgga cgggtcggct ctccgtccgt caatgccgcc gtcaacgtca gcagcaacta
     3301 cgggaaaatc aacggcaaca tcaccgtcgg gcaaagtaat tctttcagta cctcgcccct
     3361 gggcgggaaa ctcaacctga ccgttgccga tgccgaagta ttccgcaact tcctgcccgt
     3421 cggacaaacc gtcaaaggca gcctgaatgc cgccgtaacc ctcggcggca gcatcgccga
     3481 tccgcacttg ggcggcagca tcaacggcga caaactctat taccgcaacc aaacccaagg
     3541 catcatcttg gacaacggct cgctgcgttc gcatatcgcg ggcaggaaat gggtaatcga
     3601 cagcctgaaa ttccggcacg aagggacggc ggaactctcc ggtacggtcg gtatagaaaa
     3661 cagcggaccc gatgtcgata tcggcgcggt gttcgacaaa taccgcatcc tgtcccgccc
     3721 caaccgccgc ctgacggttt ccggcaacac ccgcctgcgc tattcgccgc aaaaaggcat
     3781 atccgttacc gggatgatta aaacggatca ggggctgttc ggttcgcaaa aatcctcgat
     3841 gccgtccgtc ggcgacgatg tcgtcgtatt aggcgaagtc aaaaaagagg cggcggcacc
     3901 gctccccgtc aatatgaacc tgactttaga cctcaatgac ggcatccgct tcgccggcta
     3961 cggcgcggac gttaccatag gcggcaaact gaccctgacc gcccaatcgg gcggaagcgt
     4021 gcggggcgtg ggcacggtcc gcgtcatcaa agggcgttat aaggcatacg ggcaggattt
     4081 ggacattacc aaaggcacgg tctcctttgt cggcccgctc aacgacccca acctcaacat
     4141 ccgcgccgaa cgccgccttt cccccgtcgg tgcgggcgtg gaaatattgg gcagcctcaa
     4201 cagtccgcgc attacgctga cggcaaacga accgatgagt gaaaaagaca agctctcctg
     4261 gctcatcctc aaccgcgccg gcagtggcag cagcggcgac aatgccgccc tgtccgcagc
     4321 cgctggcgcg ctgcttgccg ggcaaatcaa cgaccgcatc gggctggtgg atgatttggg
     4381 ctttaccagc aagcgcagcc gcaacgcgca aaccggcgaa ctcaaccccg ccgaacaggt
     4441 gctgaccgtc ggcaaacaac tgaccggcaa actctacatc ggctacgaat acagcatctc
     4501 cagcgcggaa cagtccgtca aactgattta ccggctgacc cgcgccatac aggcggttgc
     4561 ccgtatcggt agccgttcgt cgggcggcga gctgacatac accatacgtt tcgaccgctt
     4621 ctccggttcg gacaaaaaag actccgccgg aaacagcaaa ggaaaataag cggttttcag
     4681 acggcgcgcc gccaaaccgg acatttgaaa acctgctttt ccaccgtccg ccgccgccgt
     4741 ccgcctgcaa gggaacagaa tcgatatagt gaattaacaa aaatcaggat aaggcgacga
     4801 agccgcagac agtacaaata gtacggaacc gattcactcg gtgcttgagc accttagaga
     4861 atcgtt
//
LOCUS       DAWHSK010000150         4826 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.150, whole genome shotgun sequence.
ACCESSION   DAWHSK010000150 DAWHSK010000000
VERSION     DAWHSK010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4826
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.150"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(359..1297)
                     /locus_tag="WIC03_001503"
     CDS             complement(359..1297)
                     /locus_tag="WIC03_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ3883982.1"
                     /translation="MLELNGLCKRFGGKTVADNICLTVGRGKILAVLGRSGCGKSTLL
                     NMIAGIVRPDGGEIQLNGENITRMPPEKRRISLMFQDYALFPHMSALENAAFGLKMQK
                     MPKAEAERLAMAALAEVGLENEVHRKPEKLSGGEKQRLALARALVVRPSLLLLDESFS
                     SLDTHLRGTLRRMTAERIRNGGIPAVLVTHSPEEACTAADEIAVMHGGRILQYGTPET
                     LVKTPSCVQVARLMGLPNTNDDRHIPQHAVRFDQDGMECRVLSRTCLPESFSLSVLHP
                     EHGILWLNLDMPHAGEISGNDTVRIHIEDREIVRFR"
     gene            complement(1306..2181)
                     /locus_tag="WIC03_001504"
     CDS             complement(1306..2181)
                     /locus_tag="WIC03_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEZ3883983.1"
                     /translation="MPTVRFTESVSKQDLDALFEWAKASYGAKSCWKTLYLNRLPLGN
                     LSPEWAERVKKDWEAGCSESSDGIFLNADGWSDMGGRLQHLARTWNKAGLLHGWRNEC
                     FDLTDGGGNILFSLERAAFRPFGLLSRAVHLNGLTESDGRWHFWIGRRSPHKAVDPDK
                     LDNIAAGGVSSGELPSEAVCRESSEEAGLDQTLFPLIRPVSRLHSLRPVSRGVHNEIL
                     YVFDAVLPETFLPENQDGEVACFEKMDIGSLVATMLSGNMMHDAQLVTLDAFCRYGLI
                     DAAHPLSEWLDGIRL"
     gene            complement(2370..2446)
                     /locus_tag="WIC03_001505"
     tRNA            complement(2370..2446)
                     /locus_tag="WIC03_001505"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2410..2412),aa:Met,seq:cat)
     regulatory      2596..2695
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2786..4687
                     /gene="thiC"
                     /locus_tag="WIC03_001506"
     CDS             2786..4687
                     /gene="thiC"
                     /locus_tag="WIC03_001506"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEZ3883984.1"
                     /translation="MTTPKKTAKTSGNEARELADLSEDIGIRFKYPNSERVYLQGSRD
                     DIRVPLREIRQDDTYTAQGAEANPPIPVYDTSGVYGDPAAHIDLKQGLPHIRTAWLNE
                     RGDTEILPKLSSEYGIERAHDPKTAHLRFNQITRPRRAKSGSNVTQLHYARQGIITPE
                     MEFVAIRERLKLDELSQKSEYAKLLKQHAGQSFGANIPTRPDQITPEFVRQEIAAGRA
                     IIPANINHPELEPMIIGRNFRVKINGNLGNSAVTSSLTEEVEKMVWSLRWGADTIMDL
                     STGAHIHETREWIIRNAPVPIGTVPIYQALEKTGGIAEDLTWDLFRDTLIEQAEQGVD
                     YFTIHAGVLLRYVPMTADRLTGIVSRGGSIMAKWCLAHHRENFLYTHFDEICEIMKAY
                     DVSFSLGDGLRPGCIADANDESQFAELHTLGELTSKAWKHDVQVMIEGPGHVPLQRVK
                     ENMTEELQHCFEAPFYTLGPLVTDIAPGYDHITSGIGAANIGWYGTAMLCYVTPKEHL
                     GLPDKEDVRTGIITYKLAAHAADLAKGWPGAQLRDNALSKARFEFRWRDQFRLSLDPE
                     RAESFHDETLPAEGAKIAHFCSMCGPKFCSMKITQEVRDYADKQKAQRQGMEEKAVEF
                     VKKGAKIYS"
BASE COUNT         1185 a         1365 c         1262 g         1014 t
ORIGIN      
        1 ccgtgttggg ttctagattc ccgcttttgc gggaatgacg gcagggtggt ttcagttgct
       61 cccgataaat gccgccatct caagtctcgt cattccctca aaaacagaaa accaaaatca
      121 gaaacctaaa atccgtcatt cccgcgcagg cgggaatcta ggtctgtcgg cacggaaact
      181 tatcgggaaa aacggtttct ttagatttta tgttctagat tcccgctttt gcgggaatga
      241 cggcagggtg gtttctgttg tttcggataa attttgcagc cctgataaaa aatatggctg
      301 ctttggtaaa aaaatgccgt ctgaaaggtt ttcagacggc atttgttttt aagaagcatc
      361 agcggaagcg gacgatttcc ctgtcttcga tatggatgcg taccgtatcg tttcccgata
      421 tttcaccggc gtgcggcata tcgaggttca gccataggat gccgtgttcc ggatggagga
      481 cggacaggct gaacgattcg ggcaaacagg tacgggataa tacgcggcac tccatgccgt
      541 cttggtcgaa acgcaccgca tgttgcggaa tatggcggtc atcgttggta ttgggcagcc
      601 ccatcagtcg ggcgacctgc acgcaggacg gtgttttgac caatgtttcg ggtgtgccgt
      661 attgtagaat cctccctcca tgcatcacgg cgatttcgtc cgccgccgtg caggcctctt
      721 cgggcgaatg cgtcaccaaa acggcaggga tgccgccgtt tcggatacgt tcggcagtca
      781 tgcggcgcag cgtgccgcgc aaatgcgtgt ccaaactgga aaacgattcg tccaacagca
      841 gcagggaagg gcggacaacc aaagcgcgcg ccagtgccaa ccgttgtttc tcgcctccgg
      901 aaagtttttc aggcttgcgg tgcacctcgt tttccagtcc gacttcggca agtgccgcca
      961 tggcaaggcg ttcggcttcg gctttcggca ttttttgcat tttcaaaccg aatgccgcat
     1021 tttccagcgc actcatatgg ggaaacagcg cgtaatcttg aaacatcagc gaaatacggc
     1081 gtttttcggg cggcatacgg gtaatgtttt ccccgttcag ctgaatttcg ccgccgtccg
     1141 gccggacgat gcccgcaatc atattcagca gggtggattt tccgcagccc gaccgcccca
     1201 gtacggcgag tattttgccg cgcccgacag tcaggcagat gttgtcggca accgttttgc
     1261 cgccgaagcg tttgcagagt ccgttcagtt caagcatggc gcatcctata aacgtatgcc
     1321 gtccagccac tcggacagcg gatgggcggc atcaatcaga ccgtaacggc aaaacgcgtc
     1381 cagcgtaacc agttgcgcgt cgtgcatcat gtttcccgac aacatggtgg caaccaagct
     1441 gccgatgtcc attttttcaa aacacgcaac ctcgccgtcc tgattttcag gcaggaaggt
     1501 ttcgggtagg acggcatcga atacatacag gatttcattg tgcacacccc ggctgacggg
     1561 gcgcaggctg tgcagccggg ataccgggcg gatgagcgga aacagcgttt gatccaaacc
     1621 cgcttcttcg ctgctttcgc gacacacggc ttcagacggc aattcaccgc tggaaacacc
     1681 gccggcggca atattgtcga gtttgtcggg atcgactgct ttgtgcggac tgcgcctgcc
     1741 tatccaaaaa tgccatctgc catccgattc agtcagaccg ttgagatgga cggcgcggct
     1801 gagcagtccg aacggacgga aagcggcgcg ttcgagtgag aacaagatat tgccgccgcc
     1861 gtcggtcagg tcgaaacact cgttgcgcca tccgtgaagc agccccgcct tgttccatgt
     1921 gcgggcgagg tgctgcaagc ggccgcccat atctgaccag ccgtccgcat tcaggaaaat
     1981 gccgtctgaa gactccgagc agcctgcctc ccagtctttt ttgacgcgtt ccgcccattc
     2041 cggcgacaga ttgcccaaag gcagacggtt cagatacagc gttttccagc aactttttgc
     2101 accgtaactt gcttttgccc actcgaacag ggcatcaagg tcttgtttgc tgacggattc
     2161 ggtaaaacgg acggtcggca tagtcggctt tcttgaacat aatgcggaag attgtagcca
     2221 aaccgcctga atttgcaacc gcaaacagac ggcgggctgc cgttccgata ttgcggcagg
     2281 aaggaaaatc cggcaaaaaa cggaggcggc aggaaacagg aaacaggcaa aacaaaaccc
     2341 ctcggcattg aaccgagggg tttaatattt ggttgcggga gcaggattcg aacctacgac
     2401 cttcgggtta tgagcccgac gagctaccat gctgctccat cccgcgtcag aagatggaac
     2461 tatacggcag attttttatg ttgtcaaaca ttatttccgc gaaaaatcat aaaattttgc
     2521 cgtccggcgg tttatgcctg atttgaaata ttattttctt tacaaaagtt catgttcgtg
     2581 atttaatttt ggttaacatt gaaacagggg tgctgcctga tgttcaggcg gctgagaaat
     2641 accctttaca cccgatcggg ataatacctg cgtggggagt tttcacggat tctgtctttc
     2701 agacagcatt ggttttcaaa tgccgtctga aaacgcaaaa cgctcctgtt tctttattct
     2761 aaacgagaaa acaggagcat tttttatgac tacgccaaaa aaaaccgcca aaacttccgg
     2821 caacgaagcg cgcgaacttg ccgacttgag cgaagacatc ggtatccgct tcaaatatcc
     2881 gaactcggaa cgcgtgtatc tgcaaggcag ccgcgacgac atccgcgtgc ctttgcgtga
     2941 aatccgtcag gacgacacct atacggcgca aggcgcggaa gccaacccgc cgattcccgt
     3001 ctatgacacc agcggcgtgt acggcgatcc agcggcacac atcgacctga aacaaggtct
     3061 gccgcacatc cgcaccgcat ggctgaacga acgcggcgat accgaaatcc tgcccaagct
     3121 ctccagcgaa tacggcatcg aacgcgcaca cgatccgaaa accgcccatc tgcgcttcaa
     3181 ccaaatcacc cgcccgcgcc gcgcgaaaag cggtagcaac gtaacccagc ttcactacgc
     3241 gcgccaaggc attatcacgc ccgaaatgga gtttgtcgcc atacgcgaac gtttaaaatt
     3301 agacgaattg tcccaaaaat cggaatacgc caaactcttg aaacaacacg cagggcaaag
     3361 tttcggcgcg aacatcccaa cccgtcccga ccaaatcacg cccgaattcg tgcgccaaga
     3421 aatcgccgcc ggacgcgcga ttatccccgc caacatcaac caccccgaac tcgaaccgat
     3481 gattatcggc cgcaacttcc gcgtcaaaat caacggcaac ttgggcaatt ccgccgtcac
     3541 ttccagcctg accgaagaag tcgaaaaaat ggtgtggtcg ctgcgttggg gcgcggacac
     3601 aattatggac ttatccacag gcgcgcacat ccatgaaacg cgcgaatgga ttatccgcaa
     3661 cgcgcccgtc cccatcggca ccgtgccgat ttaccaagcg ttggaaaaaa ccggcggcat
     3721 tgccgaagat ttgacttggg atttgttccg cgacacctta atcgagcagg cggaacaagg
     3781 cgtggactat ttcaccatac acgcaggcgt gttgctgcgt tatgtgccga tgactgccga
     3841 ccgcctcacc ggcatcgtat cgcgcggcgg ttcgattatg gcgaaatggt gtttggcaca
     3901 tcatcgggaa aacttccttt acacgcattt cgacgaaatc tgcgaaatta tgaaggcgta
     3961 tgacgtatcg ttcagcctcg gcgacggttt gcgtcccggc tgcattgccg atgccaacga
     4021 cgaatcccaa ttcgccgagc tgcacacctt gggcgaattg acctccaaag cgtggaaaca
     4081 cgacgtacag gtgatgatcg aaggtcccgg ccatgtaccg ctgcaacgcg tcaaagaaaa
     4141 tatgaccgaa gagttgcaac actgttttga agcaccgttt tacaccctcg gcccgctcgt
     4201 taccgacatc gcccccggct acgaccacat cacctcgggc ataggcgctg ccaatatcgg
     4261 ctggtacggc acggcgatgc tctgttacgt tacccccaaa gaacatttgg gactgcccga
     4321 caaagaagac gtgcgtaccg gcatcatcac ctacaaactc gccgcccacg ccgccgacct
     4381 cgccaaaggc tggccgggcg cacagttgcg cgacaacgcc ctgagcaagg cgcgtttcga
     4441 gttccgctgg cgcgaccagt tccgcctaag cctcgacccc gaacgcgccg aaagcttcca
     4501 cgacgaaacc ctgcccgccg aaggcgcgaa aatcgcccac ttctgttcga tgtgcggccc
     4561 caaattctgc tcgatgaaaa tcacgcagga agtgcgcgac tacgccgaca agcaaaaagc
     4621 ccaacggcag ggcatggagg aaaaagcggt cgagttcgtc aaaaaaggag cgaagattta
     4681 cagctaaaac gtcaagcaaa aaatgccgtc tgaaacccgg aaaaaaggct tcagacggca
     4741 ttctttcgct tgtgaaaata tagtggatta acaaaaatca ggacaaggcg acgaagccgc
     4801 agacagtaca gatagtacgg aaccga
//
LOCUS       DAWHSK010000151         4753 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.151, whole genome shotgun sequence.
ACCESSION   DAWHSK010000151 DAWHSK010000000
VERSION     DAWHSK010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4753
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.151"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..180
                     /locus_tag="WIC03_001507"
     CDS             <1..180
                     /locus_tag="WIC03_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEZ3883985.1"
                     /translation="EALVVGDTAHDLHMAANAGAAAVGVATGAHSREQLLSAPHLAVL
                     DGLSELPGFLAQHYA"
     gene            complement(267..1235)
                     /gene="ispH"
                     /locus_tag="WIC03_001508"
                     /gene_synonym="lytB"
     CDS             complement(267..1235)
                     /gene="ispH"
                     /locus_tag="WIC03_001508"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEZ3883986.1"
                     /translation="MNEKTIILANPRGFCAGVDRAISIVERALEEFGAPIYVRHEVVH
                     NKFVVDNLREKGAVFIEDLAEVPPGATLVYSAHGVSKAVRQEAAERGFRVFDATCPLV
                     TKVHKEVARLDAQDCEIIMIGHKGHVEVEGTMGQLAPGKMLLVETVGDVAKLEVRNPD
                     KLAYVSQTTLSVDETKDIIAALNARFPNIRNPHKEDICYATTNRQTAVKELAEQCDIV
                     IVVGSPNSSNSNRLREVAAGIGTDAYMVDNAGYLQRAWFEGKNKVGVTAGASAPEVLV
                     QEVLATIRGWGHETVREGEGAEESIVFVLPKELRREGETKPDLCKR"
     gene            complement(1265..1759)
                     /locus_tag="WIC03_001509"
     CDS             complement(1265..1759)
                     /locus_tag="WIC03_001509"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HEZ3883987.1"
                     /translation="MSSSVSSKTRYWVLALAAIVLDQWSKWAVLSSFQYRERVNVIPS
                     FFDLTLVYNPGAAFSFLADQGGWQKYFFLVLAVAVSAYLVRAILRDEFAALGKTGAAM
                     IIGGALGNVIDRLIHGHVVDFLLFYWQNWFYPAFNIADSFICVGAVLAVLDNIVHRKD
                     GKKT"
     gene            complement(2041..2997)
                     /locus_tag="WIC03_001510"
     CDS             complement(2041..2997)
                     /locus_tag="WIC03_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISNgo3 family transposase"
                     /protein_id="HEZ3883988.1"
                     /translation="MNIIGLDISKDTIDATLHKTNGSIHYIKFKNNDDGLKQFRLWIK
                     GNRIRKVYIGMEATGIYYEKAADMLSSYYTVYVINPLKIKDYGKSRFNRTKTDKADSN
                     LIADYIKRHQDTLIPYQIPKNKALQKLINLKNQLQQQQKQIKNRLHSTEEDFIRNIHQ
                     DLIDTIQDKMEQVKIAISEQIKKQTDYNHYRNLQTIPSIGKDTASVLYAQLTEKHFKT
                     ANQFVSYAGLSPAIIQSGTSVRGRGRLSRYGNRRLKSTLYMPALCAYRFNAFPKLINN
                     LKKAGKPKMVIIVAIMRKLAKLAYYIVKTGQPYDAERHRLNQ"
     gene            complement(3296..4399)
                     /locus_tag="WIC03_001511"
     CDS             complement(3296..4399)
                     /locus_tag="WIC03_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2075 domain-containing protein"
                     /protein_id="HEZ3883989.1"
                     /translation="MAEICLITGTPGSGKTLKMVSMMANDEMFKPDENGIRRKVFTNI
                     KGLKIPHTYIETDAKKLPKSTDEQLSAHDMYEWIKKPENIGSIVIVDEAQDVWPARSA
                     GSKIPENVQWLNTHRHQGIDIFVLTQGPKLLDQNLRTLVRKHYHIASNKMGMRTLLEW
                     KICADDPVKMASSAFSSIYTLDKKVYDLYESAEVHTVNKVKRSKWFYTLPVIVLLIPV
                     FVGLSYKMLSSYGKKQEEPAAQESAATEQQAVLPDKTEGEPVNNGNLTADMFVPTLSE
                     KPESKPIYNGVRQVRTFEYIAGCIEGGRTGCACYSHQGTALKEVTELMCKDYVKNGLP
                     FNPYKEESQGQEVQQSAQQHSDRAQVATLGGKP"
     gene            complement(4400..4684)
                     /locus_tag="WIC03_001512"
     CDS             complement(4400..4684)
                     /locus_tag="WIC03_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2523 domain-containing protein"
                     /protein_id="HEZ3883990.1"
                     /translation="MKLLAALIPLLMSVAGRILTALGLMAVTYSGVDRLVAHFQQAIT
                     SSITGAPQAMLQLFYISGGGTVLNILFGAIAFILSFKQMTKLATSIGKKK"
BASE COUNT         1075 a         1201 c         1039 g         1438 t
ORIGIN      
        1 gaggcattgg tcgtcggcga tacggcgcat gacctgcata tggcggcaaa cgcaggcgcg
       61 gcggcagtcg gcgttgccac cggcgcacat tcgcgcgaac agctccttag cgcaccgcat
      121 ctcgccgtat tggacggttt gtccgaactg cccggttttc ttgcacagca ttacgcctga
      181 ttggtttccg catccggcac tcgaaacaaa tgccgtctga aggttgttca gacggcattt
      241 gttttgccca aacattcaac gcttgctcaa cgtttgcaca aatcgggttt ggtttcgccc
      301 tcgcggcgca actctttggg caggacgaac acgatgcttt cttccgcgcc ctccccttcg
      361 cgtacggttt catgccccca cccgcgtatg gttgccaata cttcctgcac caacacttcg
      421 ggcgcggacg cgcctgccgt tacgccgact ttgttcttgc cctcaaacca cgcgcgttgc
      481 aggtagcctg cattgtccac catatacgca tcggttccga tgcctgccgc cacttcgcgc
      541 aggcggttgc tgttggacga attgggcgaa ccgaccacaa tcacgatgtc gcactgttct
      601 gccaactctt tgacggcggt ttgccggttg gtcgtcgcat agcagatatc ttccttgtgc
      661 ggattgcgga tattggggaa acgcgcgttc agcgcggcga tgatgtcttt ggtttcatcg
      721 accgagagcg tggtttggct gacataggcg agtttgtcgg ggtttctgac ttcgagtttt
      781 gccacatctc cgaccgtttc gaccaaaagc attttgcccg gcgcaagctg ccccatcgtt
      841 ccttcaacct cgacgtgccc cttatgcccg atcatgatga tttcacagtc ttgggcatcc
      901 agtcgggcga cttccttatg cactttcgtc accagcgggc aagtcgcatc aaacacgcgg
      961 aaaccgcgct ccgccgcttc ttgccgcacc gccttcgata cgccgtgtgc cgaataaacc
     1021 agtgtcgcgc ccggcggcac ttccgccaag tcttcaataa acaccgcacc tttttcacgc
     1081 aggttgtcca cgacgaattt gttgtgaacg acttcgtggc gcacataaat cggcgcgccg
     1141 aattcttcca aagcacgttc gacaatactg attgcccgat ccacgccggc gcagaagccg
     1201 cgcggattgg caaggatgat ggttttctcg ttcataagtt ttgcattccg tgttcagacg
     1261 gcattcacgt ttttttgcca tctttgcgat ggacgatatt gtcaagcact gccaataccg
     1321 cgccgacgca gataaaactg tcggcaatat taaaggcggg ataaaaccaa ttttgccaat
     1381 aaaacaataa gaaatcgacg acatgaccgt gtatcaggcg atcgatgaca ttgcctaacg
     1441 caccgccgat aatcatcgcc gcacccgttt tgccgagggc tgcaaactca tcgcgcaaga
     1501 tggcgcgtac caaatacgcg ctcaccgcca ccgccagcac caaaaaaaag tatttttgcc
     1561 agccgccctg atcggcaagg aagctgaacg ccgcacccgg gttgtacacc agcgtcagat
     1621 cgaaaaagga aggaatgaca ttgacgcgtt cccgatactg aaacgacgac agcaccgccc
     1681 acttcgacca ctggtccagc acgatggcgg caagtgccaa tacccaatag cgcgttttac
     1741 ttgaaacaga tgaagacata tttttcaaca gccggtaaaa gagtaccatt ttacccgaaa
     1801 accccctttc ctgtacccga aacggcaaat gccgtaatct taaaacccgt cattcccgac
     1861 aacaccgtaa tctcgaaacc cgtcattccc gcgtagatga tatgttgccc gtcaacacaa
     1921 aataaaaaac aaagttgcaa tatactgatt tatattgtta tttttattta cgtttattta
     1981 cgatatgcaa atgcacggtt acacaaatat attcgcgtaa ccgtttaatt ttgttgaatt
     2041 ttattgattc aatcggtgtc tttccgcatc gtaaggctgg ccggttttaa caatataata
     2101 ggcgagcttc gccagtttgc gcatgatggc aacgataatt accatctttg gcttacccgc
     2161 ttttttcaga ttatttatta atttcggaaa tgcgttaaaa cggtaagcac aaagggcggg
     2221 catatacagc gtacttttta atcgtctgtt tccgtatcgg ctcaatctgc cccgacctct
     2281 tacgcttgtc cctgattgta tgatggcggg acttaatccg gcataggata caaactggtt
     2341 tgcggtttta aaatgttttt ctgtcagttg cgcataaaga actgatgcgg tgtctttgcc
     2401 tatgctcggg atggtttgaa gattgcggta atggttatag tccgtttgtt ttttgatttg
     2461 ttcggatatg gctattttta cctgttccat cttgtcctgt atggtatcta tcaagtcttg
     2521 atgtatgttc cttatgaagt cttcttcagt gctatgaaga cggtttttaa tttgcttctg
     2581 ctgttgctgt aattggtttt taagattaat cagtttttgc agtgctttgt ttttgggtat
     2641 ctgatacggt atcaatgtat cttgatgcct ttttatgtag tctgctatca ggtttgaatc
     2701 tgctttgtcg gttttggtac ggttaaacct gctttttccg tagtccttga tttttaaggg
     2761 attaataacg taaacagtat agtaggaaga aagcatatct gctgcctttt cgtaatagat
     2821 gcctgttgcc tccatgccga tatagacttt tctgattctg tttcccttta tccacaatct
     2881 aaactgtttt aatccatcat cattattctt aaatttaatg taatggatac ttccgtttgt
     2941 tttatgcaat gttgcgtcta tggtgtcctt tgagatgtcc agcccgatta tattcattgg
     3001 tattttcctt atttatacag ccttgatacg gctaggatga tattcaattt cgaggatgga
     3061 taaaggcagc cggcatttct acgcgtctgt tttaatacat tgcgggattt gctgcctgac
     3121 tgccttagcc cttgctttgc gcgaaacaaa gacccgtaaa ccgtctatat tcaaacggtt
     3181 tacgggtctt ttttctctct tgccgttttc ttcagtttgc cgatccgacc acgcccccgc
     3241 cgattccttc aaacggtttc ccgcgttctt cccaattatc gtacattagg ttctgctacg
     3301 gttttccgcc caatgtggca acttgcgccc tgtccgaatg ttgctgcgcg ctttgctgaa
     3361 cttcctgccc ttggctttct tctttgtatg ggttaaacgg caagccgttt tttacatagt
     3421 ccttgcacat caactccgtc acttctttca atgccgtccc ttgatgcgaa tagcaggcgc
     3481 atccggttct tccgccttct atacagcctg ctatatattc aaaggttctt acctgcctta
     3541 caccgttata aatcggcttg ctttcgggtt tttcggacaa tgtcggaaca aacatatctg
     3601 cggtaaggtt gccgttattt accggctcgc cttctgtttt atccggaagt actgcctgct
     3661 gttctgttgc cgccgattct tgtgctgcgg gttcttcctg tttttttccg taactgctca
     3721 acattttata ggacaggccg acaaacacgg gaatcagcaa tactattact ggcagagtgt
     3781 aaaaccactt tgaccgcttg accttattta cggtatgaac ttccgctgat tcgtacaagt
     3841 cataaacttt tttatccagt gtatagatac tggagaatgc gcttgatgcc atttttacgg
     3901 gatcgtccgc gcatattttc cattctaaaa gcgtacgcat acccatcttg tttgaagcga
     3961 tgtggtaatg tttccgtaca agcgttctaa gattttgatc tagaagctta ggaccttgag
     4021 tcaaaacaaa tatatcaatg ccctgatgtc tgtgcgtatt cagccattgg acattttcag
     4081 ggatttttga acctgccgag cgtgccggcc atacgtcttg agcttcatct acaatgacaa
     4141 tagacccgat attttcgggc ttctttatcc attcgtacat atcatgcgcc gatagctgct
     4201 catctgtcga tttcggcagc ttttttgcgt ccgtttctat gtaggtgtgc ggtattttca
     4261 agccttttat gttcgtaaat actttacggc gtatgccgtt ttcatcaggc ttaaacattt
     4321 catcattcgc catcatggaa accattttta atgttttccc tgaaccgggc gtgccggtta
     4381 tcaaacagat ctctgccatt tattttttct tcccgattga ggttgctagt tttgtcattt
     4441 gtttgaatga cagaataaag gcgatcgcgc caaacaggat attaagaacg gtgccgccac
     4501 cgcttatata aaagagctgc aacatcgctt gaggcgcgcc cgttatgcta ctggttatcg
     4561 cctgctgaaa atgggctacc aatctatcta cacccgaata agtaacagcc atcaagccta
     4621 atgcagtcaa tatacggcct gcgacgctca tcaacagcgg aatcaatgcg gccaacaatt
     4681 tcatttgcta tccctttctt aaaaggcacg gttgcctcat taaaaaaatg tttcttaatc
     4741 tgaaagattt tgc
//
LOCUS       DAWHSK010000152         4729 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.152, whole genome shotgun sequence.
ACCESSION   DAWHSK010000152 DAWHSK010000000
VERSION     DAWHSK010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4729
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.152"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            165..944
                     /gene="pgeF"
                     /locus_tag="WIC03_001513"
     CDS             165..944
                     /gene="pgeF"
                     /locus_tag="WIC03_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEZ3883991.1"
                     /translation="MKTITETLNLAPKGKNFLTADWPAPANVKTLITTRNGGVSQGAY
                     QSLNLGTHVDDNPEAVRRNREIVQQQVGLPVAYLNQIHSTVVVNAAEALGGTPDADAS
                     VDDTGKVACAVMTADCLPVLFCDRAGTTVAAAHAGWRGLAGGVLQNTIAAMKVPPVEM
                     MAYLGPAISADAFEVGQDVFDAFCTPMPEAAAAFEGIGGGKFLADLYALARLILKREG
                     VGGVYGGTHCTVLERDTFFSYRRDGATGRMASLIWLDGNAV"
     gene            996..1475
                     /locus_tag="WIC03_001514"
     CDS             996..1475
                     /locus_tag="WIC03_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883992.1"
                     /translation="MNKLFLTAAVLMLGACGFHLKGADGISPPLTYRSWHIEGGQALR
                     FPLETALYQASGRVDDAAGAQMTLRIDSVPQNKETYTVTRAAVINEYLLILTVEAQVL
                     KRGEPVGKPMTVSVRRVLAYADNEILGKQEEEAALWAEMRQDAAEQIVRRLTFLKAE"
     gene            1477..2475
                     /locus_tag="WIC03_001515"
     CDS             1477..2475
                     /locus_tag="WIC03_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEZ3883993.1"
                     /translation="MAAHIGRIDTDAPLKPLYVIHGEEELLRIEALDALRAAAKKQGY
                     LNREVYTADNAFDWNELLQTAGSAGLFADLKLLELHIPNGKPGKTGGEALQDFAARLP
                     EDTVTLVLLPKLEKTQLQSKWFAALAAKGEVWEAKPVGAAALPQWIRGRLDKIGLGIE
                     ADALALFAERVEGNLLAARQEIDKLALLYPKGHTVNIDEAQTAVANVARFDVFQLAGA
                     WMKGDVPRVCRLLDGLQEEGEEPVLLLWAVAEDVRTLIRLAAVLKQGQGVQSVRNSLR
                     LWGDKQTLAPLAVKRISVVRLLDALKTCAQIDRIIKGAEDGDAWTVFKRLVVSLAE"
     gene            2596..3015
                     /locus_tag="WIC03_001516"
     CDS             2596..3015
                     /locus_tag="WIC03_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883994.1"
                     /translation="MDNKTKLRLGGLILLTTAVLSLIIVLIVDSWPLAILLAAVIVAA
                     AAGGFVWTSRRQQRQFIERLKKFDIDPEKGRINEANLRRMYHSGGQHQKDAITLICLS
                     QKCSVDEAHAMFKKRPTRQEINQMAAKQSRGQKRPHR"
     gene            3090..3605
                     /locus_tag="WIC03_001517"
     CDS             3090..3605
                     /locus_tag="WIC03_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEZ3883995.1"
                     /translation="MMILKTAPLKRRFAAMLYEMLLVGAATCLAALFAGIAAIFLNPV
                     SIAVSALVTSILIMGSWWLYFRANWHGQGQTLAMRTWKIGLCDLNGIQPSLHLLRLRF
                     IWACIFIVFIPMLAYAGLRHFLGIPPKGAAGAALIWLILPWGFALLNPDRQFLYDFLA
                     GTRLVAVKGKP"
     gene            complement(3689..4573)
                     /locus_tag="WIC03_001518"
     CDS             complement(3689..4573)
                     /locus_tag="WIC03_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HEZ3883996.1"
                     /translation="MSSGNIHIHSMTGFANAAAECGSKRINLDLRAVNHRFLDIQIRM
                     PDDLRYLESGIREKISSHIARGKVECKIQIQDAENGNQSLELNRDLVGQLAEINKDLR
                     KHYDLAKLGVADILRFPGVLASQRENADALAKVIADLTDSALKDFTAARRREGKKLGE
                     HLLQRLESMEEIIDALSELFPTLLETHKEKIRARLAEAVGSIDNDRLQQEFALFIQKS
                     DIDEEFSRLRTHIAEVRRIVTEHKGSSGKRLDFLMQELNREANTLGSKSIATECTQAS
                     VELKVLIEQMREQVQNIE"
BASE COUNT         1067 a         1255 c         1318 g         1089 t
ORIGIN      
        1 taagggcatc attcccgcac cggcaagaat ccgaacccga acgtttgaaa acaatcccga
       61 atctccgaat tcccgcctgt gcgggaatga cgaaaaaaca aacatccatt tgccccgaag
      121 gcagttaatc aaccctttcc gccacacacc tattccaata tccaatgaaa accatcacag
      181 aaaccctaaa tctcgccccg aaaggcaaaa acttcctgac cgccgattgg cccgcgcccg
      241 ccaatgtgaa aaccctgatt accacgcgca acggcggcgt gagccaaggt gcgtatcaga
      301 gtttgaacct cggtacgcac gtcgacgaca atcccgaagc cgtgcgccgc aaccgcgaaa
      361 tcgtgcaaca gcaggtcgga ttgcccgttg cctacctcaa tcaaatccac agtaccgtcg
      421 tcgtcaatgc tgccgaagcg ttgggaggca cacccgatgc ggacgcttcc gtagacgaca
      481 cgggcaaagt tgcctgtgcc gtgatgaccg cagactgcct gcctgttttg ttttgcgaca
      541 gggcgggtac gacggttgcc gccgcacacg cgggctggcg cggtttggcg ggcggcgtat
      601 tgcaaaacac cattgccgcg atgaaggttc cgcctgtcga aatgatggcg tatctcggcc
      661 ccgccatcag tgcggatgcg tttgaagtcg gacaggacgt gtttgatgcg ttctgcacgc
      721 ccatgcccga agccgccgcc gcatttgaag gcataggtgg cggcaaattc cttgccgacc
      781 tttacgcgct cgcccgcctg attctgaagc gcgaaggcgt gggcggcgta tatggcggca
      841 cgcattgtac ggttttggaa cgggatactt tcttttccta ccgccgcgac ggagcgacag
      901 ggcgtatggc gagcctgatt tggctggacg gcaatgccgt ctgaacacgc cgctgatata
      961 atctaccgac tttgtgcttt tgagaaaggc aagccatgaa caaactgttt cttactgccg
     1021 cagtgctgat gctgggcgcg tgcggtttcc acctgaaagg tgcagacggc atttctccgc
     1081 cgctgaccta ccggagctgg cacatcgaag gcggacaggc attgcggttt cctttggaaa
     1141 cagcgctgta tcaggcttcg ggcagggtgg acgatgctgc cggcgcgcag atgaccctgc
     1201 gtatagacag cgttccccaa aacaaggaaa cctacaccgt tacccgtgcg gcagtcatca
     1261 acgaatatct tttgatattg acggttgaag cgcaggtatt gaaacgcggc gagccggtcg
     1321 gcaaaccgat gaccgtgtcc gtccgccgcg tccttgctta tgccgacaac gagatcttgg
     1381 gcaaacagga agaggaagcg gcattgtggg cggaaatgcg gcaggatgcc gccgaacaga
     1441 ttgtccgccg cctgaccttt ctgaaggcgg aatgacgtgg cggcacatat cggacgcatt
     1501 gatacggacg cgcctttgaa acccctgtac gtcatccacg gcgaggaaga actgttgcgt
     1561 atcgaggcat tggacgcatt gagggcggcg gcgaagaaac aaggttacct taatcgggaa
     1621 gtttatacgg cagacaatgc cttcgattgg aacgaactgt tgcaaaccgc aggcagtgcg
     1681 ggtctgtttg ccgatttgaa gctgttggaa ctgcatatcc ccaacggaaa acccggcaaa
     1741 accggcggcg aggcgttgca ggattttgcc gcccgcctgc cggaagatac ggtaacgctg
     1801 gttttgctgc ccaaactgga gaaaacccag ctccagtcca aatggtttgc cgcattggcg
     1861 gcaaaggggg aagtgtggga agccaaaccg gtcggcgcgg cggccttgcc ccaatggata
     1921 cgcggacggc tggacaaaat cggtttgggt atcgaggcag acgcattggc actgtttgcc
     1981 gagcgcgtgg aaggcaattt gttggcggcg cgtcaggaaa tcgacaagct cgcgctgctg
     2041 tatccgaaag ggcataccgt caatatcgat gaggcgcaaa ccgccgttgc caatgttgcc
     2101 cgctttgacg tttttcagct tgccggcgca tggatgaagg gcgatgtccc gcgcgtatgc
     2161 aggttgttgg acggattgca ggaggaaggc gaagaaccgg tactgcttct gtgggcggtt
     2221 gccgaagacg tgcggacgct gattcggctt gccgctgtcc tgaaacaggg gcagggtgtc
     2281 caatccgtac gcaacagtct cagactttgg ggcgacaagc agacgctcgc gccgcttgcg
     2341 gtcaagcgga tttccgtcgt ccgcctgctt gacgcgctca aaacctgcgc ccaaatcgac
     2401 cgaatcatca aaggcgcgga agacggcgac gcatggacgg tattcaaacg gcttgtcgtg
     2461 tcgctggcgg aataaagcgg taatccccaa aatccgaaaa tactgtaaaa taccgttaat
     2521 cctgaaaagt attcaccaat ccgtccgaaa acatttcaga cggcacgacc acctcaataa
     2581 aggaacatta accctatgga caataagacc aaactgcgct tgggcggcct gattttactg
     2641 accaccgccg ttttaagcct cattatcgta ttgattgtcg attcctggcc gcttgccatc
     2701 ctgcttgccg ccgtcatcgt cgccgccgct gcgggcggct ttgtttggac atcccgccga
     2761 cagcaacgcc agtttatcga acgcctgaaa aaattcgaca tcgatcccga aaaaggcaga
     2821 atcaacgagg caaacctgcg ccgtatgtac cacagcggcg gacaacacca gaaagatgcg
     2881 attaccctga tctgcctgtc gcaaaaatgt tcggtggacg aggcgcacgc tatgttcaaa
     2941 aaacgcccga cacgtcagga aatcaatcaa atggcggcaa aacagtcgcg cggtcagaaa
     3001 cgtccgcacc gttaaccaca tgagacatat ctgcatgaat gccgtctgaa gcctgttggc
     3061 gtttcagacg gcatattccg attgaaaaga tgatgatact gaaaaccgcc ccgctcaaac
     3121 gccgctttgc cgccatgctg tacgaaatgc tgctggtcgg tgcggcaacc tgtttggcag
     3181 cattgtttgc cggtattgcc gccatttttc tgaatcccgt ttctattgcg gtttctgcat
     3241 tggtaacgag tatcctgata atgggatcat ggtggcttta tttccgtgcc aactggcacg
     3301 ggcagggaca gaccttggcg atgaggacgt ggaaaatcgg tttgtgcgac cttaacggca
     3361 tacagccgtc tctacactta ctccgcctgc gctttatttg ggcgtgcata ttcatcgtct
     3421 tcattcctat gcttgcttat gccggattgc gccacttcct cggcattccg cccaagggcg
     3481 cggccggcgc ggcattgatt tggctgattt taccgtgggg gttcgccctg ctgaatcccg
     3541 atcggcagtt tctgtatgat tttcttgcag gaacaagatt ggtggcggtc aaaggaaagc
     3601 cttaagcctt tataccgcaa aggtttcaaa ctgaaaaaat gccgtctgaa agggctttca
     3661 gacggcattt gcttatcggg gaaaccgatt attcgatatt ctgcacctgt tcgcgcatct
     3721 gttcgatcag gactttcagc tcgaccgagg cttgggtgca ttcggtagcg atggatttgc
     3781 tgcccaaagt gttggcttcg cggtttaatt cctgcatcag gaagtccagc cgtttgccgc
     3841 tgctgccttt gtgttcggta acgatacggc gcacttcggc aatgtgggtg cgtaggcggc
     3901 tgaactcttc gtcgatgtcg gatttttgga taaagagggc aaattcctgt tgcaggcggt
     3961 cgttgtcgat gctgccgacc gcttcggcaa ggcgggcgcg gattttttct ttatgtgttt
     4021 ccaacagggt aggaaagagt tcgcttaatg catctatgat ttcttccatg ctttcgaggc
     4081 gttgtaacag gtgctcgccc aattttttcc cttcgcgccg gcgtgcggcg gtaaagtcct
     4141 tcagtgccga atcggtcaaa tcggcaataa cctttgccaa tgcgtccgca ttttcccttt
     4201 gacttgccaa tacgcccgga aagcgcaaaa tatcggcaac gcccagtttt gccaaatcat
     4261 aatgcttgcg gaggtctttg ttgatttcgg caagctgtcc gaccagatcg cgattcagtt
     4321 ccaaagactg attgccgttt tccgcatctt gaatttggat tttgcattcg actttgccgc
     4381 gtgcgatatg ggatgaaatt ttctcgcgga taccgctttc caaatagcgc aaatcgtcgg
     4441 gcatcctgat ttgaatatct agaaaacggt ggttgaccgc acgcaggtcg agattgatgc
     4501 gtttgctgcc gcactctgcc gctgcgttgg caaatccggt catgctgtgg atgtggatat
     4561 ttccgctgct catgtcgttc tccgaagccc gttaaaatgg aatcaatata tcacatctgt
     4621 atggcggcaa gcgttttcgg gtgtgaaaaa ttgaagattt tgcagcggca gattggaatc
     4681 acgcgctttt gttgctgcaa ggaagggaaa tgtatagtgg attaaccaa
//
LOCUS       DAWHSK010000153         4707 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.153, whole genome shotgun sequence.
ACCESSION   DAWHSK010000153 DAWHSK010000000
VERSION     DAWHSK010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4707
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.153"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            390..1106
                     /locus_tag="WIC03_001519"
     CDS             390..1106
                     /locus_tag="WIC03_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003685045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ3883997.1"
                     /translation="MTMHETTDRLFEIAKEQGVLKPADIAERLNISQQALKNWESRGI
                     AAKALPEVAKAFGVSETWLRTGEGSRTAPVLIDPDLPHEVKDIHRPMTWSSNDPLPDD
                     DYVFVPYLKESCFKGGVGTYEIPDYNGYRLPFGKSTLKRKGINPDNVFCCTLTGDSME
                     EKIAEDAAIAVDTGETAIRDGKIYAFAQDGMFRVKYLIRQPGNSVLIRSHNSGFYGDE
                     NAPLDSLTVIGRVFWWSVLD"
     gene            1209..1634
                     /locus_tag="WIC03_001520"
     CDS             1209..1634
                     /locus_tag="WIC03_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883998.1"
                     /translation="MNFLGVQGELKVHELHHRTATLCFEWLGEVSAPRRKENYKDLKP
                     NVLYDFDGSGKHFANPDARYLLPVGSSGLILKHIQIDDEDTLLRLWCARNIPMPHRLS
                     KIPMLRQYYLSKAWHEIYAINQHLRKTKLIVDVAYDPTD"
     gene            1878..2078
                     /locus_tag="WIC03_001521"
     CDS             1878..2078
                     /locus_tag="WIC03_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3883999.1"
                     /translation="METITIPKENFDALLNLARTAVALNENFIPKINYGASTIPAAGF
                     AALNEFGIARSTVESLEILKIK"
     gene            2146..2505
                     /locus_tag="WIC03_001522"
     CDS             2146..2505
                     /locus_tag="WIC03_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884000.1"
                     /translation="METQEKKFVPASKQIPTVNSGWLDRELGEAMSEAVRACLAHGKQ
                     SEITVKLKIQPQNIQSGTVKISHDVATKLPKEKREGGIVFATPDGNIQADDPAQGKLK
                     LKQVSNTSSTLKMVRSN"
     gene            2530..3384
                     /locus_tag="WIC03_001523"
     CDS             2530..3384
                     /locus_tag="WIC03_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdQ family protein"
                     /protein_id="HEZ3884001.1"
                     /translation="METQENMIKTALQAAQKPFFEFAPNNTPLVFTPDQDGGWRYKSH
                     PELMQNPYRKCGKFLMHDTASLIKFVQKHKQDGTQIYIDADFKSGRIDVTAVINGHTQ
                     QAPGWLDFYAVYTPQHTTSASNWLNNNAHRMNQMEFSHFLTNNARNIVSKNPGNENSV
                     YPTAAEVLDFALNLEYTEKTTFKQGYREQDGRINFTFQSEDSGQTEKNLKMFERFGIE
                     FTPHQGGASYFVEALLKFRIDKNSGALVLWYELQQIDAVIEQAAKDISEAVQKAFPEI
                     DIYFGVIA"
     gene            3454..3669
                     /locus_tag="WIC03_001524"
     CDS             3454..3669
                     /locus_tag="WIC03_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884002.1"
                     /translation="MTITEEYSIDIRVTSEQGKNDYGYPTERYGCDIINTDGELLVGI
                     EPEYKTPFAAVRKALICLTKDNLKNAA"
     gene            3704..3964
                     /locus_tag="WIC03_001525"
     CDS             3704..3964
                     /locus_tag="WIC03_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884003.1"
                     /translation="MKYLIRTALLAVAAAGIYACQPQSEAAVQVKAENSLTAMRLAVA
                     DKQAEIDGLNAQIDAEIRQREAEELKDYRWIHGDAEVPELEK"
     gene            3961..4254
                     /locus_tag="WIC03_001526"
     CDS             3961..4254
                     /locus_tag="WIC03_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884004.1"
                     /translation="MNFQEENENIKNDIEINLLHSAEADSVEAMMDLAVYGLAAMVAK
                     HNKTRGEQLVLQAIAGHMRKLLAALPETESLVQTGKVYRRLEDLIFKTYLPEQ"
     gene            4257..4448
                     /locus_tag="WIC03_001527"
     CDS             4257..4448
                     /locus_tag="WIC03_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884005.1"
                     /translation="MNTNQDTIPFGGNLVICCSTGNGGDGRFSCLITDQIPDLNLVRS
                     GQAFPMFIYEYETREKRKA"
     gene            4584..>4707
                     /locus_tag="WIC03_001528"
     CDS             4584..>4707
                     /locus_tag="WIC03_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HEZ3884006.1"
                     /translation="MNINELGARIDRPTIRELIAYATCRNRPISNSTLLRMEKDG"
BASE COUNT         1455 a         1210 c         1031 g         1011 t
ORIGIN      
        1 aatattaaaa attatcgtca tcaatgcggc ttccccagcc cgcggcgagc tttccacgac
       61 aggctttttt tatttgggtt ggtcgtctgg tttctttgag ttcaaaaaca ggtcgggata
      121 cttaagtttt attcgtgcag gtatcccgcg cttcgtccaa ttgaaaacgc attgagggct
      181 attccctgtt attcgaccaa cttccgcgta actgccgatt gattgcaaca ggcgtttgtc
      241 ttcattgact cttttatcca taaataaacc aaatgtttaa atctaatgct aatattaaac
      301 actatgttta gataaaaatc aagtcttgtg taacaacatt ttgtttaaat atgggagaat
      361 aattaagcca accgcgaata agattaaaaa tgacaatgca cgagacaact gacagacttt
      421 ttgagatagc caaagagcag ggagttttaa agccggctga catagcagag cgtctgaata
      481 tcagccaaca ggctttgaaa aactgggaaa gtcgtggcat agcggcaaag gcgctgcctg
      541 aagtagcaaa agcattcggt gtatctgaaa catggctgag aacaggtgaa ggcagccgaa
      601 ccgcgcccgt ccttattgac cccgacctac cccacgaagt caaagacatc caccgcccga
      661 tgacgtggag cagcaacgac ccgctgcccg acgatgatta tgttttcgtc ccctacctca
      721 aagagagctg cttcaaaggc ggagtaggca cgtatgaaat ccccgattac aacggctacc
      781 gcctgccgtt cggcaaatcc acgcttaaac gcaaaggcat caatcccgac aacgtgtttt
      841 gctgcaccct gaccggcgac agtatggagg aaaaaatcgc agaagacgcg gcaattgccg
      901 tagatacggg cgaaaccgcc atacgcgacg gcaaaatcta cgccttcgcc caggacggta
      961 tgttccgcgt gaagtacctg atacggcagc ctggcaacag cgttctgata cgcagccaca
     1021 acagcggttt ctatggcgac gaaaacgccc ccttggacag cctgaccgtt atcggcaggg
     1081 tattttggtg gagcgtgctg gattgaaaaa tgttgctatt acgacgagat aatattgata
     1141 gggctttcaa gattgtcaaa aataggcgtt ttgattcccc ttggtggcct ggtgagtacg
     1201 atgccggtat gaatttcctt ggcgtacaag gagagttgaa ggttcacgag ctgcatcaca
     1261 gaacagcaac cctgtgcttt gagtggcttg gcgaagtatc tgcgccgcgc agaaaagaga
     1321 actacaaaga cctcaagcca aatgtattgt atgactttga tggttccggt aagcattttg
     1381 ccaaccctga cgcaaggtat ctattgcccg tcggttcaag cggcttaatc ttaaaacata
     1441 tccaaatcga tgacgaagac actctgttgc ggctatggtg cgcacgaaat atcccaatgc
     1501 cacaccggct atctaaaatc ccgatgcttc ggcagtatta tctaagcaag gcatggcacg
     1561 aaatttatgc tatcaaccaa cacctaagga aaacgaagct gatagttgat gtggcatacg
     1621 accccacaga ttaaacaacc cgcccgcatt atgcgggctt ttttcatgcc ccgccgaacc
     1681 tgaaaacaac acaaaaccga caaagccgcc ttaataggcg gtttttttgc gcctgcgaaa
     1741 tctcaaaaat aaattctttt aataatcaaa atgtttataa accttaaaca aaaatcttaa
     1801 acagattgtt gcatattatt aaacgctatg tttaaataca cccatcgaaa caaacaacag
     1861 ccacatggag taccgacatg gaaaccatca ccatccctaa agaaaatttc gacgctttgc
     1921 taaatctcgc aagaaccgcc gtcgccctga acgaaaattt tattccaaaa ataaattacg
     1981 gagcatccac cataccagca gccggctttg ccgcacttaa cgagtttgga atagcccgct
     2041 ccaccgtgga atctttggaa attctcaaaa ttaaataacc tcctcggtat tgtcatcccg
     2101 tgataccggc aacctaacta acctctcttt aaaggaaaat caaaaatgga aacccaagaa
     2161 aagaaatttg ttcctgcctc caagcaaata ccgaccgtaa atagcggctg gttagaccgt
     2221 gaattaggcg aagccatgag tgaagcggtg cgtgcctgcc ttgctcacgg taaacagtca
     2281 gaaattactg tcaagctcaa aatccaaccc caaaacatcc aaagcggaac ggtaaaaatc
     2341 agccacgatg tagcaaccaa actgcccaaa gaaaaacgcg aaggcggcat cgtctttgct
     2401 acacctgacg gcaacatcca agccgacgat ccggcacaag gaaaattgaa actcaaacaa
     2461 gtttccaata cgtcaagcac gttgaaaatg gtcagatcca actaactcaa acacaaaagg
     2521 aaatctaaaa tggaaaccca agaaaacatg attaaaaccg ccttacaagc agctcaaaaa
     2581 cctttttttg agtttgcacc gaacaatact ccgcttgtat tcacaccaga ccaagacggt
     2641 ggctggagat acaaatcaca ccccgaattg atgcaaaacc cgtaccgcaa gtgcggcaaa
     2701 ttcctcatgc acgataccgc cagtcttatt aagtttgtac aaaaacacaa acaggacggc
     2761 acacaaatct acattgatgc cgatttcaaa tcagggcgta ttgatgtcac cgccgtcatc
     2821 aatggccata cgcaacaagc tccgggctgg ctcgattttt atgccgtata caccccgcaa
     2881 cacactacat cggcatccaa ctggctgaac aacaatgcgc accgcatgaa tcagatggag
     2941 ttttcccatt tcctgaccaa caatgcccgc aacatcgtat ccaaaaatcc cggtaacgaa
     3001 aattccgttt acccgaccgc cgccgaagta ttggatttcg cgctcaatct cgagtacacc
     3061 gaaaaaacca ccttcaaaca aggctatcgt gaacaggacg gcagaatcaa ctttaccttc
     3121 caatccgaag actcagggca aacagagaaa aacctcaaaa tgtttgaacg tttcggcatc
     3181 gagttcaccc cgcatcaagg cggtgcatcc tactttgttg aagccctgtt gaaattccgc
     3241 atcgacaaaa acagcggcgc actcgtcctg tggtacgaat tgcagcaaat cgatgctgta
     3301 atcgaacaag cggcaaaaga catttccgaa gccgtacaaa aagccttccc tgaaatcgac
     3361 atctatttcg gcgtaatcgc ctaaaaaaaa gtcgtctgaa atcgtgaacc ttcagacgac
     3421 ctccacaaaa acccaaatcc gaaagacccc aaaatgacca tcacagaaga atactccatc
     3481 gacatccgag taacctcaga acaggggaaa aacgactacg gctaccccac cgaacgctac
     3541 ggatgcgaca tcatcaacac agacggggag ctacttgtcg gcatagagcc agagtacaaa
     3601 accccctttg ccgccgtcag aaaggcgctc atctgtctga caaaagacaa cctcaaaaac
     3661 gctgcctgat attaagccgt taccccaata atgaggaatc aaaatgaaat acttgatccg
     3721 caccgcctta ctcgcagtcg cagccgccgg catctacgcc tgccaaccgc aatccgaagc
     3781 cgcagtgcaa gtcaaggctg aaaacagcct gaccgctatg cgcttagccg tcgccgacaa
     3841 acaggcagag attgacgggt tgaacgccca aatcgacgcc gaaatcagac aacgcgaagc
     3901 cgaagaattg aaagactacc gatggataca cggcgacgcg gaagtgccgg agctggaaaa
     3961 atgaactttc aggaagaaaa cgagaacatc aagaatgaca tagagattaa tctcttgcat
     4021 tccgctgaag ccgacagcgt ggaggcaatg atggacttgg cggtttacgg gctggcggcg
     4081 atggttgcca aacacaacaa aacgcgcggc gagcagcttg tgttacaggc gattgccggt
     4141 catatgcgga agcttttggc cgcgcttccc gaaaccgagt cattggtgca gaccgggaag
     4201 gtttaccgcc gacttgaaga tttgattttt aaaacgtatt tgccggagca gtaaagatga
     4261 acacgaatca agatacgatt ccatttggcg gcaaccttgt gatttgctgt tcgacgggaa
     4321 acggcgggga cgggcggttt tcttgtctga ttaccgatca gataccggat ttaaatcttg
     4381 tcagaagcgg gcaggcgttc ccgatgttca tttatgagta cgaaactaga gaaaaaagga
     4441 aagcctgatg gacaccctat taaaaatcat catcgcgctg tcattttccg gcgctgaggc
     4501 attggtggta tggcttttca tcacggccgc cgatgccgta ttacgccgct tccagaaacc
     4561 gagtcattac aggagagtaa aaaatgaaca tcaacgaact gggcgcaagg atagaccgcc
     4621 cgaccatccg cgaactgatt gcctacgcaa cctgccgcaa ccgccccatt tcaaactcga
     4681 cactgctgcg tatggaaaaa gacgggc
//
LOCUS       DAWHSK010000154         4687 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.154, whole genome shotgun sequence.
ACCESSION   DAWHSK010000154 DAWHSK010000000
VERSION     DAWHSK010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4687
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.154"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(416..952)
                     /locus_tag="WIC03_001529"
     CDS             complement(416..952)
                     /locus_tag="WIC03_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEZ3884007.1"
                     /translation="MDLREVKLGGETIYEGGFVSISRDKVRLPNGNEGQRIVIRHPGA
                     ACVLAVTDDEKVVLVRQWRYAANQATLELPAGKLDVAGEDMAACALRELAEETPYVAD
                     SVRLLYSFYTAVGFCNEKMYLFEAEGVRLGSTLANDEDEITETVLMSKEEVRQALAND
                     EIKDGKTLIGLQYWLMKD"
     gene            complement(986..1342)
                     /gene="folB"
                     /locus_tag="WIC03_001530"
     CDS             complement(986..1342)
                     /gene="folB"
                     /locus_tag="WIC03_001530"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEZ3884008.1"
                     /translation="MDKIFLHGIKADTLIGVYGWERERLQTLIVDLDIGVPEKAGSDD
                     DIANTVHYAEVCETLRRHLKEQDFLLLEALAEYIADLVLGYFGAVWVRVKIVKPGILE
                     GVREVGVEIERGKRED"
     gene            1398..2000
                     /gene="plsY"
                     /locus_tag="WIC03_001531"
     CDS             1398..2000
                     /gene="plsY"
                     /locus_tag="WIC03_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEZ3884009.1"
                     /translation="MFNIPAVAVSYLIGSLSFAVIVSKYYGMDDPRTYGSGNPGATNV
                     LRSGKKKAAALTLLGDAAKGLVAVLLARVLQEPLGLSDSAIAAVALAALVGHMWPVFF
                     GFKGGKGVATALGVLLALSPATALVCALVWLVMAFGFKVSSLAALTATIAAPLAALFF
                     MPHTSWIFATLAIAILVLLRHKSNIVKLLEGRESKIGGSR"
     gene            2077..2922
                     /locus_tag="WIC03_001532"
     CDS             2077..2922
                     /locus_tag="WIC03_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HEZ3884010.1"
                     /translation="MLSYRHAFHAGNHADMLKHFTLFLVLQYFNRKDKPYWYIDTHGG
                     AGVYNLEGSEAQKVGEYRQGIALLRQAKNLPTELNGFIAHIQKILPSPELYCGSPWLA
                     QSLTRVGDKLRLFELHPTDFVHLQNNMGEAGLGKRGQVLREDGYKGLISLLPPPPRRA
                     TVLIDPPYEEKQDYRRVTETLKAALKRFESGCYLIWYPCLSREESRKLPEELKKLLPD
                     NYLHAELHVHAPKADGFGMHGSGMFVINPPYLLAEQLAANLPALTRLLAQDEGARYLL
                     DSKIR"
     gene            3168..4142
                     /locus_tag="WIC03_001533"
     CDS             3168..4142
                     /locus_tag="WIC03_001533"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HEZ3884011.1"
                     /translation="MDTLTRFLPEHLQQNQLPEALGGVLLSVVSACTEINAKVRLGAL
                     AGVLGMAGTGNIQGEDQKKLDVIANNIMIDTLKANPAVAGLASEEEDSFVSAGENGRY
                     LVLFDPLDGSSNIDVNISVGTIFSILEKPEGALATESFLQTGRQQLAAGYILYGPQTQ
                     LVFTFGHGVYMFTLNAENEFVLTKEKPKVPESTKEFAINMSNRRHWLPPVQQYIDELL
                     AGETGTRGKNYNMRWVASMVAEIHRILMRGGVFMYPQDKRDPSKPGKLRLMYEANPMS
                     LILEQAGASASNAYQAMLDIQPENLHQRVAVFMGSSEEVAYLDRLHAK"
     gene            complement(4285..4503)
                     /locus_tag="WIC03_001534"
     CDS             complement(4285..4503)
                     /locus_tag="WIC03_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HEZ3884012.1"
                     /translation="MFPEYRDLISKLKQENSRFARLFDEHNELDDKITGLVNNPVTSG
                     AETIDELKKAKLKLKDELYAILQKAAGK"
BASE COUNT         1206 a         1402 c          985 g         1094 t
ORIGIN      
        1 ctcaaattca aattgggctt ttaggtctat aacaaagaaa aagccttggt aggataaacc
       61 taccaaggct ttttcttaaa aaaacgctct attaccgtgc ctaaaaatgc aaaaaagtaa
      121 ccgtcataac ccttttgcca aaatcaatat catcatccga tgaatgaatt gacggtatat
      181 aagaaagaat taataagctc ataaaccggt aagaaaaatc agtaacaaag atattcatta
      241 ttaaccattt taaaaattat catgttcgtt tgggtaatta ttataccctt ttccctctta
      301 ttactaaagc acgaatgact ttaaaggacg cgcccagaga caagccgaaa aggcatatct
      361 catccgccaa acaaaatgcc caccgactta ctccggcagg cagttcaaca ccctgtcaat
      421 ccttcatcag ccagtattgc aaaccgatta atgtcttgcc gtctttaatt tcatcgtttg
      481 ctaatgcctg acggacttct tctttcgaca tcaatacggt ttccgtaatc tcgtcttcgt
      541 cattggcaag cgtactgccc aaacgcacgc cttccgcttc gaacagatac attttttcgt
      601 tgcaaaaacc gaccgccgta taaaaactgt aaagcaggcg cacgctgtcg gcaacataag
      661 gcgtttcttc agccagttct ctcagcgcac acgctgccat atcctcgccc gccacatcca
      721 gcttgcccgc aggaagttcc aatgtcgcct gatttgccgc ataccgccac tgccgtacca
      781 aaacgacctt ttcatcatcc gttaccgcca acacgcacgc cgctcccgga tgacggatga
      841 cgatacgctg cccttcattg ccgttgggca acctgacctt atccctgctg atgctgacaa
      901 aaccgccctc gtaaatggtt tcgccgccta attttacttc cctcaaatcc acttcatatc
      961 tcctttccgt ttcctattct gccgttcaat cttcacgctt gccgcgctcg atttccacgc
     1021 caacctcgcg cacgccttcc aaaatacccg gcttgacgat tttcacgcgt acccacaccg
     1081 cgccgaaata tcccaaaacc aaatcggcaa tatattccgc caacgcttcc aaaagcagga
     1141 aatcctgttc tttcagatgt cggcgcagtg tttcgcatac ctcggcataa tgtaccgtat
     1201 tggcaatatc gtcgtccgaa cccgctttct cgggaacgcc gatgtccaaa tcgacaatca
     1261 gggtctgcaa ccgttcgcgt tcccagccgt acacgccgat aagcgtatcc gccttgatgc
     1321 cgtgcaaaaa gattttatcc ataatccgcc ccgttttttg ctaaaataac cgcaccattc
     1381 taagtaaagt accacaaatg ttcaacatac cggctgttgc cgtttcctat ctgatcggct
     1441 cactttcttt cgccgtcatc gtttccaagt attacgggat ggacgacccg cgcacctacg
     1501 gatcgggcaa tcctggggcg accaatgttt tacgcagcgg caaaaaaaag gcggccgcgc
     1561 tgacgctctt gggcgatgcc gccaaaggtt tagttgccgt tttgcttgca cgcgtgcttc
     1621 aagaaccgct cggtttatcc gacagcgcaa tcgctgccgt cgcactcgcc gcgctggtcg
     1681 ggcatatgtg gccggtgttt ttcggattta agggcggcaa aggcgtggca acggcattgg
     1741 gcgtgcttct ggcactctct cccgcaactg ccttggtctg cgcgttggtt tggcttgtta
     1801 tggcattcgg cttcaaggtg tcctcccttg ccgcattaac cgccacaatc gccgcccccc
     1861 ttgccgcact gttttttatg ccgcatactt cttggatttt cgcaaccctc gcaatcgcca
     1921 tattggtgtt gctccgccac aaaagtaata tcgtcaagct gctcgaaggc agagaaagca
     1981 aaatcggcgg cagccgctga tttcccatcc atccctgttt atgccgtctg aaccccgctt
     2041 cagacggcat ccgaatatcc cgccgtttta ccgactatgc tcagttacag acacgcattc
     2101 cacgccggca accacgccga tatgctcaaa cacttcacct tgtttttagt gctgcaatat
     2161 ttcaaccgca aagacaagcc ctactggtac atcgacacgc acggcggcgc gggtgtgtac
     2221 aatctcgaag gcagcgaggc acaaaaagtc ggcgaatacc ggcaaggcat cgccctactt
     2281 cgacaagcaa aaaacctgcc aaccgagtta aacggcttta tcgcgcacat acaaaaaatc
     2341 ctgccctcgc ccgaacttta ctgcggttct ccgtggctgg cgcaatcgct gacccgcgtc
     2401 ggcgacaaat tacgcctgtt tgagctgcac cccaccgact ttgtccatct gcaaaacaat
     2461 atgggcgaag caggattggg caaacgcgga caagtgttgc gcgaagacgg ttacaaaggg
     2521 ctgatttccc tgctgccgcc cccgccgcgc cgcgccaccg tcctcatcga cccgccctat
     2581 gaggaaaaac aggactaccg gcgtgtaacg gaaacgctga aagccgcttt aaaacggttt
     2641 gagtccggct gttatctcat ttggtatccc tgcctcagcc gcgaagaaag ccgcaagctg
     2701 cccgaagaat tgaaaaaact cctgcccgac aactaccttc acgccgaact tcacgtccac
     2761 gcgccgaaag ccgacggttt cggtatgcac ggcagcggaa tgttcgtcat caatccgccc
     2821 tatctgttgg cggaacaatt ggcggcaaac ctccccgccc tgacgcggct gctggcgcaa
     2881 gacgaaggcg cgcgctacct tttggacagc aaaatccgct gaccccgatg ccgtctgaaa
     2941 cccttcagac aacatctccc gtcaaataaa caaaaccgga cgatataccg gcagtcggca
     3001 cttttaagaa taaatatccc ctcatccgcc tgccgcgttc cgacttgccc catgccgttt
     3061 ttttacacta caatccccta gactatcctg caaaacgcac caaaggaata cgccacaata
     3121 acctgccgcc cttcagacgg cacaaacctt tccgaaacga gcaaactatg gacacactga
     3181 cccgattcct ccccgaacac ctgcaacaaa accagctgcc cgaagcactc ggcggcgttt
     3241 tactgtccgt tgtttccgcc tgcaccgaaa tcaacgccaa agtccgcctc ggcgcactgg
     3301 ccggcgtatt gggtatggcg ggcacgggca atattcaggg cgaagaccaa aaaaaactgg
     3361 atgttatcgc caacaacatc atgattgaca cactcaaagc caaccctgcc gttgccggtt
     3421 tggcaagcga ggaagaagac tctttcgtaa gtgccggtga aaacggacgc tatctcgtcc
     3481 tattcgaccc tttagacgga tcgtccaata ttgatgtcaa catttccgtc ggtacaattt
     3541 tctccatcct tgaaaaaccc gaaggcgcat tggcaaccga atcattcctt caaacgggca
     3601 gacagcagct tgccgcaggc tatatcctct acgggcctca aacccagctc gtattcacat
     3661 tcggacacgg cgtatatatg ttcacgctca atgccgaaaa cgaatttgtg ctgaccaaag
     3721 aaaaaccgaa agtacccgaa agtaccaaag aatttgccat caatatgtcc aaccgccgcc
     3781 actggctgcc ccccgtccaa caatacatcg acgagctctt ggcgggcgaa accggcacgc
     3841 gcggcaaaaa ctacaatatg cgctgggtag ccagcatggt tgccgaaatc caccgcatcc
     3901 tgatgcgcgg cggcgtgttc atgtatccgc aagacaaacg cgacccgtcc aaacccggca
     3961 aactgcgcct gatgtacgaa gccaacccta tgagcctgat actcgaacag gcgggcgcat
     4021 cggcaagcaa cgcatatcaa gccatgctcg acatacagcc cgaaaacctg caccaacgcg
     4081 tcgccgtctt catgggcagc agcgaagaag tcgcctatct cgaccgcctg cacgcaaaat
     4141 agacagacag ccgttcacac aagtacacgg cggcggcaat ccgataccgc cgccgttatt
     4201 tcaaacagca caagccttct ggtcaaatga caaaaatgcc gtctgaaaac atttcagacg
     4261 gcatttcaaa aactcaaacc cgaattattt tcccgctgct ttttgcagga tggcgtacaa
     4321 ctcgtctttc agtttcaatt tggctttttt cagctcatcg atggtttccg caccgctggt
     4381 aaccggattg ttgaccagac cggtaatttt atcgtccagc tcgttgtgtt cgtcgaacag
     4441 acgggcgaag cgggaatttt cctgtttcaa tttggaaatc aaatcacggt attctgggaa
     4501 catatcgctt tcctctcttg gtttacggtt aaaaagtcaa actgaatctt tacattaatt
     4561 ataacaaaga taatattttt atccagtgtt ttatcggata cggcaaaatt ttcttttaac
     4621 accgccgtcc ggttgataaa aacgttgaca atatcacgct ttacgcataa attttcagcc
     4681 tttccta
//
LOCUS       DAWHSK010000155         4651 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.155, whole genome shotgun sequence.
ACCESSION   DAWHSK010000155 DAWHSK010000000
VERSION     DAWHSK010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4651
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.155"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..302)
                     /locus_tag="WIC03_001535"
     CDS             complement(<1..302)
                     /locus_tag="WIC03_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T cell/B cell stimulating protein TspB"
                     /protein_id="HEZ3884013.1"
                     /translation="MFLILGRNFLKIILCFSFFVPKFALASVNVPGKFDRVEVYDDGR
                     YLGIRGSDDKRRRIWKGVFDRESGRYLTSEAQDLKVRHVSTGASSTGKVSSVVSSSV"
     gene            complement(649..951)
                     /locus_tag="WIC03_001536"
     CDS             complement(649..951)
                     /locus_tag="WIC03_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884014.1"
                     /translation="MGYRVGMNCFDTRLQADDYLLSSLPPTVTEEGKIIRPERVGDKW
                     ILNGKPVTLSYPECSNFEQIKQGSYVGSTVLILFVVIFGFRLLINFLKDMGKVGTD"
     gene            complement(979..1209)
                     /locus_tag="WIC03_001537"
     CDS             complement(979..1209)
                     /locus_tag="WIC03_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884015.1"
                     /translation="MNIVKKYAVKALLAAGIFTPAIVMADGFDAAAIGTQVANVIMGF
                     VAMVSAVGMAAITVILAIQGFKMAWSMIKSVK"
     gene            complement(1447..1647)
                     /locus_tag="WIC03_001538"
     CDS             complement(1447..1647)
                     /locus_tag="WIC03_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884016.1"
                     /translation="MKFEERFIVQDLETHDFIYPDPFGDVGFTQNIKSAGQFESYEDA
                     LNSGINEMGGGFQIFQFFVKSE"
     gene            complement(1651..1962)
                     /locus_tag="WIC03_001539"
     CDS             complement(1651..1962)
                     /locus_tag="WIC03_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884017.1"
                     /translation="MFNQTQTVTYPATFLGAKKFKGEIDGSNIDTCSVLVATPLPAQS
                     GNAVGFTAAQMKFGDSKNFSRLENLKYPCEVMVMVEMTSTGKGMVPSLIDFQVAEKPK
                     G"
     gene            complement(1974..3188)
                     /locus_tag="WIC03_001540"
     CDS             complement(1974..3188)
                     /locus_tag="WIC03_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEZ3884018.1"
                     /translation="MSEVEYFSHFISDGKGKLLEIPQRRGKQDGVFVDWISFTFHEDT
                     LLKVSGCPLFSDAEYMYVLSRKLEEILGFGITRKCKSRGNKFYESMYRLGSDDVDYGE
                     VHFGGQRNTVLVELKGTGCSVASPGWELRLKQFLDDSIRTRITRIDLALDFFDGEYTP
                     DQALLDHDNGFFDNSNQRPKSETIGTAWRNEDGSGKTFYVGRKKNSRFVRVYEKGRQL
                     GDKESKWVRFEIQFNYGDIEIPLDILINQGSYFCGAFPICRKFKNMPVPERFDQRKKT
                     LNLTFEHKLHYAKNAVGKLVNFMIEMGFDNSEIVESLKADSGFPKGLEPEKYALEMLR
                     DGLKHGFIHEQPDIDLEIELDELGVIAFKNSDKFDREKRLFSPDYDVEKERKYQEYLS
                     KVYHQNVDYDYF"
     gene            complement(4182..>4651)
                     /locus_tag="WIC03_001541"
     CDS             complement(4182..>4651)
                     /locus_tag="WIC03_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEZ3884019.1"
                     /translation="GEEAWDIIGGGEEDSVLFHTWHEFPTINEKTEAELVKKWTAIRE
                     AREAVTAAIEPLRADKTVGSSLQAEAEITAPEEMAGYLNALGEELRFAMLVSKAEVKV
                     GSELAVAAKASDGEKCERCWHYTRDVGAVAGYETVCKRCAENVGGEGETRHYA"
BASE COUNT         1387 a         1140 c          787 g         1337 t
ORIGIN      
        1 actgatgaag atacaaccga actaacttta cccgtacttg atgctccagt agatacatgc
       61 ctaactttta aatcttgagc ttctgaagtt aaatatcttc ccgattctct atcaaataca
      121 cctttccaaa ttcttcttct tttgtcatct gaacctcgaa tacctaaata tctgccatca
      181 tcataaactt caaccctatc aaatttaccc ggaacattta ctgatgccaa tgcaaattta
      241 ggtacaaaaa aactaaagca taggataatc tttaaaaaat ttcgccctag gataagaaac
      301 ataacatttt gcttcatcta atttaattaa taatgtagaa gaatcaggga attgaatttc
      361 tataaaatct ctatcataac gatgaaagaa attcctaaaa atatcaaatg aaaacgaagt
      421 tttaaaaacc ccttttctaa tcaattccct ataataaatt aacgcttgtg gtaaatctct
      481 ataaatatgt gaatcagttt gaaatttaac taaatgatat tcagaaataa acataattcg
      541 gaccttttaa aacactctaa aaagccgatt ctaccaaccg ttaccaaaac agccaagcaa
      601 cagacaaagc caagaagaaa ccgagaagaa accaaaaatc aataatcatc aatcagtacc
      661 aaccttgccc atgtctttta agaaattaat cagaagcctg aaaccgaaaa tgactacgaa
      721 cagaattaga accgtcgaac cgacataaga accttgcttt atctgctcaa aattggaaca
      781 ttccggataa gacaacgtaa ccggctttcc gttcaaaatc catttatcgc ccaccctttc
      841 cggcctgatg atttttcctt cttctgtaac agtaggagga agagacgaca ataaatagtc
      901 gtctgcctgc aatcttgtat caaaacagtt catgccgaca cgataaccca ttcacgcgcc
      961 cctctttctt cactgccgtt atttgacaga tttaatcatg ctccaagcca ttttgaagcc
     1021 ttggattgcc aaaatgacgg taatggccgc catacccacg gcggaaacca tcgcaacgaa
     1081 acccatgatt acattcgcca cttgcgtacc aatcgcggct gcatcaaagc catctgccat
     1141 aacaatagcc ggtgtgaaga taccagctgc caacaatgct tttacagcat attttttaac
     1201 gatgttcatc gtttttttcc tttttttgat atttaaaata agacgacttc ttgacttgct
     1261 tcatccggac gaagtctttt ccgaatctcg tttttagccg ataaaataga ggattgcgaa
     1321 aagaaaaaga aacatacaga ccacccagcc gataattagt gttgcaaggt tcattttcat
     1381 gatatttttc ctttgttgcg ggctttgtga aaggttaaca gaccgcccgc cgagcctgtt
     1441 tttcttttat tccgatttta cgaagaactg aaatatctgg aatcctccgc ccatttcatt
     1501 tatgcctgaa ttcaacgcat cttcgtaact ttcaaattga cctgctgatt taatattttg
     1561 agtaaacccc acatcaccga acggatcggg ataaataaag tcatgcgttt ctaaatcttg
     1621 aactatgaaa cgttcttcaa atttcataaa tcaacctttc ggcttttccg ccacctgaaa
     1681 atcaattaat gacggaacca tgcctttacc tgtcgaagtc atttcaacca ttaccataac
     1741 ttcgcacgga tatttgagat tctctaacct tgagaaattc ttactatccc cgaacttcat
     1801 ttgtgctgcc gtaaatccaa cagcattacc cgactgtgcc ggtaaaggtg ttgcaaccaa
     1861 tacggaacaa gtgtcaatgt tagagccatc aatttcacct ttgaattttt tggctcccaa
     1921 aaaagttgca ggataagtta cagtttgagt ttgattaaac attttgattt cctttaaaaa
     1981 taatcataat ctacattttg atgataaact ttacttaaat attcctgata tttcctttct
     2041 ttctcgacat cataatcagg actaaaaagc cttttttccc tatcgaattt gtcagaattt
     2101 ttaaaagcaa taacccccaa ttcatcaagt tcaatttcca aatcaatatc cggctgttca
     2161 tgaataaaac cgtgtttcaa accgtccctt aacatttcca gagcatattt ttcaggttct
     2221 aatcctttgg gaaatcccga atctgccttt aaagattcca caatttcgct attatcaaaa
     2281 cccatttcaa tcatgaaatt gaccagtttt ccaaccgcgt ttttcgcgta atgcaattta
     2341 tgctcgaaag ttaaattaag cgttttcttt ctctgatcaa acctttcggg aaccggcata
     2401 tttttaaatt ttctacaaat tggaaaagct ccacagaaat acgaaccctg atttattaaa
     2461 atatccaagg gtatttctat atctccataa ttaaactgga tctcgaacct tacccatttg
     2521 ctttctttat ctccaagctg cctgcctttc tcataaacac gaacaaaacg agaatttttc
     2581 ttgcgaccta cataaaatgt cttgccgctc ccgtcctcat tccgccaagc cgtaccgatc
     2641 gtttcagatt tcggcctttg attgctgtta tcaaaaaaac cattatcgtg atctaacaac
     2701 gcctgatccg gcgtgtactc tccatcaaaa aaatcaagtg ctaggtcaat tcgcgttatt
     2761 cttgtcctta tcgaatcatc gagaaactgc tttagcctca actcccaacc cggacttgca
     2821 acgctgcaac cagtaccttt caactcaact aaaacagtat tgcgctgacc tccgaaatgc
     2881 acctctccat aatcaacatc atccgaacct aacctataca tggattcata gaatttgttg
     2941 ccccttgatt tgcatttgcg cgttatgcca aaacctagaa tttcttccag ctttctgctt
     3001 aatacataca tgtattcagc atcagaaaat aaagggcaac cggaaacttt cagtaaagta
     3061 tcttcatgga atgtgaatga aatccaatca acaaaaaccc cgtcttgctt acctcttcgc
     3121 tgcggaattt ctaaaagctt cccttttccg tccgatataa agtgtgagaa atattcaact
     3181 tcactcattc gttcactact cttggaattg gtttttaatt tcgctccccc cctgttagtc
     3241 aagggggggc tttcagccgt ttcccgtctt ccgcgctaaa gcgcgtacga cggtcaacga
     3301 ccgaaagcca aatcctgaca aacttttaaa gatcaacaaa aaagaccaca accatccgtt
     3361 gtgataatca ctggaaaatt cgaaccaacc gaatctatat aatcgaacgc ctgataaagc
     3421 tttgaaaaat tctcttgatt aatgagttta tgcggttcgc catgtctgaa ctgataaaaa
     3481 cacaaaacac aagaatctga ttttttaaat attctccaat aggaacaaga aaatattaca
     3541 tttgctactg acataaaaaa gcccctttca cttggctgtc aaaggggaat gttaagaaaa
     3601 gtaatgcgcc cctttgatag agcgcatcat ataaagaaaa gtaatgcgcc cctttgatag
     3661 agcgcatcat ataaggcggg aatccagacc tgtccgcaca gaaacttatc ggataaaaac
     3721 agttgctcaa acccctcgtt ctagattccc actttcgtgg gaatgacggt tcagttgcgt
     3781 tccgacaaca ccgtaatctc gaaatccgtc attcccgcac aggcgggaat ccagacccct
     3841 gacgcggcgg gaatctatcg gaaatgactg aaaccccgag attctagatt cccactttcg
     3901 tgggaatgac ggttcagttg cgttccaaca acaccgcaat ctcgaaatcc gtcattcccg
     3961 cacaggcggg aatccagacc cacgacgcgg cgggaatcta tcggaaatga ctgaaacctc
     4021 gagattctag attcccactt tcgtgggaat gacggttcag ttgcattccg acaacaccgt
     4081 aatcttgaaa cccgtccgcc attatgaaga caaattgcgg cacaaaaaat gccgtctgaa
     4141 atgctgttcg gcggtttcag acggcatttg ctcaaacttt atcaggcgta atggcgcgtt
     4201 tcgccttctc cgccgacatt ctctgcacag cgtttgcaga cggtttcata gcctgcaacc
     4261 gcgcccacat cgcgggtgta gtgccagcag cgttcgcatt tttcaccatc actggcttta
     4321 gcggcaacgg caagttcgct gcctactttc acttctgctt tagacaccag catggcaaag
     4381 cgcaattctt cgcccaaggc gttcaggtag ccggccattt cttccggcgc ggtgatttcg
     4441 gcttcggctt gcaaggacga accgacggtt ttgtcggcgc gcaaaggctc gatggcggcg
     4501 gttaccgctt cgcgggcttc gcggattgcc gtccattttt tcaccagttc ggcttcggtt
     4561 ttttcgttga tggtcgggaa ctcgtgccaa gtatggaaga ggacgctgtc ttcttcgccg
     4621 ccgccgatga tgtcccacgc ttcttcgccg g
//
LOCUS       DAWHSK010000156         4651 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.156, whole genome shotgun sequence.
ACCESSION   DAWHSK010000156 DAWHSK010000000
VERSION     DAWHSK010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4651
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.156"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..454)
                     /locus_tag="WIC03_001542"
     CDS             complement(<1..454)
                     /locus_tag="WIC03_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEZ3884020.1"
                     /translation="MTTPKLIIFDWDGTLADTTQPIIDTMRRSFAECGFPPPEAERVR
                     SLIGYSLPEIIRDLLEMPSETAIADITRTYSAHYLNPNNRNMTLFPDALPCLDKLKAQ
                     GYWLAVATGKGRAGLDNAISQTATGGYWLATACAGEYPSKPSPEMVFGI"
     gene            614..2770
                     /locus_tag="WIC03_001543"
     CDS             614..2770
                     /locus_tag="WIC03_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEZ3884021.1"
                     /translation="MSVFRINMTAATVLAALSSSVFAAQTADLETVHIKGQRSYNAIA
                     TEKNGDYSSFAATVGTKIPASLREIPQSVSIITNQQVKDRNVDTFDQLARKTPGLRVL
                     SNDDGRSSVYARGYEYSEYNIDGLPAQMQSINGTLPNLFAFDRVEVMRGPSGLFDSSG
                     EMGGIVNLVCKRPAKAFQGHAAAGFGTHKQYKAEADVSGSLNSDGSVRGRVMAQTVGA
                     SPRPAEKNNRHETFYAAADWDINPDTVLGAGYLYQQRHLAPYNGLPADANNKLPSLPQ
                     HVFVGADWNKFKMHSHDVFADLKHYFGNGGYGKVGMRYSDRDADSNYAFAGSKLGMKT
                     PAGRPGCNTADDKACAVGLGTEIKQKALAFDASYSRPFRLGNTANEFVIGADYNRFRS
                     TNEQGRTTLYARGGLALNEFRSIPQVDLIANARKGVRGYSHTVATENLDEFGIYGKST
                     FHPADGLSLIGGGRLGHYKIESGEGKTLHKASKTKFTGYAGAVYDLNDNNSLYLSLSQ
                     LYTPQTNLDADGKLLKPRQGNQFEVGYKGSYMDDRLNARVSFYRMKDKNAAAPLNPNN
                     KKTRYAALGKRVMEGVETEISGAVTPKWQIHAGYSYLHSQIKTASNSRDDGIFLLMPK
                     HSANLWTTYQVTPELTIGGGVNAMSGITSSAGMHAGGYATFDAMAAYRFTPKLKLQIN
                     ADNIFNRHYYARVGGANTFNIPGSERSLTANLRYSF"
     gene            2840..3772
                     /locus_tag="WIC03_001544"
     CDS             2840..3772
                     /locus_tag="WIC03_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEZ3884022.1"
                     /translation="MLSQLEYGIAKENRPNRQADRFAGSVLPLAASLTHKPNIMKPIP
                     TDTFQPAILPQAFETEIKSTCTGRIYRIQTATLGEMPSEGYPVLFVLDGEAFFPALFN
                     IMQSLMPNPVTRSNAPCLIVGIGYTTGSVRDLAQRAADYTPPLGDNATADERQQFGQA
                     DRFSAFIDSELTAFLESRYTLNRNETAVFGHSFGALFGLYSLLSHRRFRRHWLVSPSI
                     WWHNRRILDFMPSENRLNGIDVCLNIGALERGSGCKRREERDMAGLSERMAAELDRHG
                     AAVFFREYPNADHGNVSFYSLTDCVEYLRKAWQR"
     gene            complement(3909..4058)
                     /locus_tag="WIC03_001545"
     CDS             complement(3909..4058)
                     /locus_tag="WIC03_001545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884023.1"
                     /translation="MHDTDSTKGKNGIFQSIGIKTALSLQKYQIDKPGKEIRPCRLKK
                     RPPVR"
     gene            complement(4206..>4651)
                     /locus_tag="WIC03_001546"
     CDS             complement(4206..>4651)
                     /locus_tag="WIC03_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEZ3884024.1"
                     /translation="IAFVSLEDLSGQVEIMVGGQTLENCADCLKADQVLIIESKVSRD
                     DYGGGDGLRILANQVMTLQMARERYARSLSLALAPHHDIGKLVQLLAAHQLPDTPRIP
                     LQLSYANEKASGRLQVPPKWTVTPSSALFGELETLLGSRSVRVNW"
BASE COUNT         1074 a         1309 c         1263 g         1005 t
ORIGIN      
        1 agattccgaa taccatttcg ggcgagggtt tggagggata ttcccccgcg caggcggtgg
       61 cgagccaata gccgccggtg gcggtttggc tgatggcgtt gtccaaaccc gcccgccctt
      121 tgcccgtggc gacggcaagc cagtatcctt gtgctttgag cttgtccaga cagggcaggg
      181 catcgggaaa gagcgtcata ttgcggttgt tgggattgag gtaatgtgcg gaataagtgc
      241 gtgtgatgtc ggcaatggcg gtttcagacg gcatttcgag caggtcgcgg atgatttcgg
      301 gcaggctgta gccaatcagg ctgcggacgc gttccgcttc gggcggcgga aaaccgcatt
      361 cggcgaagct gcggcgcatg gtgtcgatga tgggttgggt cgtatcggca agcgtgccgt
      421 cccagtcgaa gatgatgagt ttgggcgtgg tcatggcagg ttgtttacgg tgaaaagtca
      481 gattttatgc ggaaaacgca gacgtgtcgc attttcgaca aaatttgtcg gctgcgcgat
      541 atgtttttcc gaacaagccg cgttgtgctt tattaaaata gaaccattat catttatgtg
      601 aatgggacag tttatgtcag ttttccgcat caatatgacc gccgccacgg ttttggcagc
      661 actttcgtct tcggtttttg ccgcacaaac ggcggatttg gaaaccgtcc atattaaggg
      721 tcagcgttcg tacaacgcga ttgccaccga gaaaaacggc gattacagct cgtttgccgc
      781 caccgtcggc acaaaaatcc ccgcttcttt gcgcgaaatt ccgcaatctg tcagcatcat
      841 caccaatcaa caggtcaaag accgcaatgt tgatacgttt gaccagttgg cacgcaaaac
      901 gcccggcctg cgcgtgttga gcaacgacga cggacgctct tcggtttacg cgcgcggtta
      961 cgaatacagc gaatacaaca tcgacggcct gcccgcgcag atgcagagta tcaacggcac
     1021 gctgcccaat ctgtttgcct tcgaccgtgt ggaagtgatg cgcgggccga gcggcttgtt
     1081 cgacagcagc ggcgagatgg gcggcatcgt aaatctggtg tgcaaacgcc cggccaaagc
     1141 gttccaaggt catgctgcgg cagggttcgg tacgcacaaa caatataaag ccgaggcgga
     1201 cgtatcgggc agcctcaatt cagacggcag cgtgcgcggc cgcgtgatgg cgcagaccgt
     1261 cggcgcgtct ccgcgtcccg ccgagaaaaa caaccggcac gaaaccttct acgcggcggc
     1321 ggattgggac atcaaccccg atacggtttt gggcgcgggc tatctttacc agcaacgcca
     1381 cctcgcgccg tacaacggct tgccagccga tgccaataac aaattaccgt ccctgccgca
     1441 acacgtattt gtcggcgcgg attggaacaa atttaaaatg cacagccacg acgtgttcgc
     1501 cgatttgaaa cattacttcg gcaacggcgg ctacggcaaa gtcggtatgc gctattccga
     1561 ccgcgatgcc gactccaact atgcctttgc cggcagcaag ctgggcatga aaaccccggc
     1621 aggccgcccg ggctgcaata cggctgacga caaagcctgc gcggtgggtt tgggtacaga
     1681 aatcaaacaa aaagccctcg cgtttgacgc cagctacagc aggcctttcc gcttgggcaa
     1741 tacggccaac gaatttgtca tcggcgccga ttacaaccgc ttccgcagca ccaacgaaca
     1801 aggccgtact actttatatg cacgcggcgg cctggcttta aacgagttcc gcagcatacc
     1861 gcaggttgat ttgattgcca acgcgcgcaa aggcgtgcgc ggttacagcc ataccgtcgc
     1921 taccgaaaac ctcgacgaat tcggcattta cggcaaatcc accttccatc ctgccgacgg
     1981 gctgtcgctt atcggcggcg gacgtttggg acactataaa atcgagtcgg gcgaaggcaa
     2041 aaccctgcac aaagccagca aaaccaagtt caccggctac gcaggcgcgg tttacgactt
     2101 gaacgacaac aacagcctct acctgagcct gtcccagctc tacacaccgc aaaccaacct
     2161 cgatgccgac ggcaagctgc tcaaaccgcg ccaaggcaac cagtttgaag tcggttacaa
     2221 aggcagctac atggacgacc gcctcaatgc ccgagtttcg ttctaccgca tgaaagacaa
     2281 aaacgccgcc gcaccgttga acccgaacaa caaaaaaacc cgttacgccg cattgggcaa
     2341 acgcgtgatg gaaggcgttg agaccgaaat cagcggcgcg gttacaccga aatggcaaat
     2401 ccatgcaggt tacagctatc tgcacagcca aatcaaaacc gcctccaatt cacgcgacga
     2461 cggcatcttc ctattgatgc ccaaacacag cgcaaacctg tggacgactt accaagttac
     2521 gcccgagctg accatcggcg gcggagtgaa cgcgatgagc ggcattactt catctgcagg
     2581 gatgcatgca ggcggttatg ccacgttcga tgcgatggcg gcataccgct tcacgcccaa
     2641 gctgaagctg caaatcaacg ccgacaacat cttcaaccgc cattactacg cccgcgtcgg
     2701 cggcgcgaac acctttaaca ttcccggttc ggagcgcagc ctgacggcaa acctgcgtta
     2761 cagtttttaa agaccaatat gccgtctgaa acggcagccg cagcatcatc aaaccacaac
     2821 aagctgcgct gcatacccta tgctctcaca actggagtat ggcattgcga aggaaaacag
     2881 accgaaccgg caggcagacc gctttgccgg ttcggtttta ccgcttgccg ccagtctgac
     2941 ccacaagccg aacatcatga aacccatacc gaccgacaca ttccaacctg ccatactgcc
     3001 ccaagccttt gaaaccgaaa tcaaatccac ctgcacgggg cgaatctatc ggattcagac
     3061 ggcgacactc ggcgaaatgc cgtctgaagg ctatcccgtc ctcttcgttt tggacggcga
     3121 agcctttttc cccgcacttt tcaacatcat gcagtcgctg atgcccaacc ccgttacccg
     3181 aagcaacgcc ccctgcctga ttgtcggtat cggctacacg acaggcagtg tgcgcgattt
     3241 ggcacaacgt gccgccgact acacgccgcc gcttggagac aacgccacag cagacgaacg
     3301 gcagcagttc ggacaggcag accgcttctc cgcctttatc gacagcgagc tgaccgcctt
     3361 tttagaaagc cgctacaccc tcaaccgcaa tgaaaccgcc gtattcggac actcgttcgg
     3421 cgcactgttc ggactgtatt ccctgctttc ccaccgccgt ttcagacggc attggctcgt
     3481 atccccatcg atttggtggc acaacaggcg gatactcgat tttatgccgt ctgaaaaccg
     3541 gctaaacggc atcgatgtct gcctcaacat cggcgcgctc gaacggggta gcggttgcaa
     3601 acgcagggaa gaacgcgata tggcaggact gtccgaacgc atggcggcag agttggacag
     3661 gcacggggcc gccgtctttt tccgggaata tccgaatgcc gaccacggca atgtctcgtt
     3721 ctactcgctg accgattgcg tcgaatattt gaggaaggct tggcagaggt agggggaatt
     3781 aaatatatga ctgctttgtt ttgcatcgga aaataacaag agctacctaa gggttattgc
     3841 tccctttctc attttatttt gatataaaaa tccctgcttc aggccgtctg aaacagggat
     3901 aggttaattt aacggacggg tgggcgtttt ttcaggcggc acggtctgat ttctttgccc
     3961 ggtttgtcga tttggtattt ttggagtgaa agggcggttt tgataccgat actttggaaa
     4021 atgccgtttt tccctttggt gctgtctgtg tcatgcacac ggatatcaat cgtcccggtt
     4081 gctctattta gactggcgaa ataaccaaaa aatacgtttt tcttggcagt tacttttatt
     4141 caatcattcg aataaagtac gaatgaaacc ggtttttggg cttcagacgg catttatatt
     4201 ttgggttacc agttgacgcg caccgaccgg ctgccgagca atgtttccag ttcgccgaac
     4261 aatgcggaac tcggcgtaac cgtccatttc ggcggcactt gaagcctgcc cgacgctttt
     4321 tcgttggcat acgacagttg cagcgggatg cgcggcgtgt cgggcagttg gtgggcggcg
     4381 agcagttgta ccagtttgcc gatgtcgtga tgcggggcga gggcgaggct gaggcttcgg
     4441 gcgtagcgtt cgcgcgccat ttgcagggtc atgacttggt ttgccagaat acgcagcccg
     4501 tcgccgccgc cgtagtcgtc gcggctgact ttggattcga taatcagcac ttggtcggct
     4561 ttgaggcagt cggcgcagtt ttccaacgtc tgaccgccga ccatgatttc aacctgtccg
     4621 ctcaaatctt caaggctgac gaaggcgatt t
//
LOCUS       DAWHSK010000157         4624 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.157, whole genome shotgun sequence.
ACCESSION   DAWHSK010000157 DAWHSK010000000
VERSION     DAWHSK010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4624
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.157"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..691)
                     /locus_tag="WIC03_001547"
     CDS             complement(<1..691)
                     /locus_tag="WIC03_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEZ3884025.1"
                     /translation="MELNEFLDKAYAVLRRLDAVLPPEPGHTDWNALAFRWQSAGKKG
                     FLEHLPDPHTFPLVRLAGVGRQTELLVRNTEQFIVGRPANNVLMSGARGTGKSSLVKA
                     LLHEYADKGLRLIEVDKSDLIGLPYLLTLLKERPEKFIVFCDDLSFESGDETYKALKT
                     ALDGGLSQRCANVLVYATSNRRHLMPEYFDENAGTTGMRGEIHQKEAVEEKISLSDRF
                     GLWLSFYPFDQN"
     gene            complement(699..1316)
                     /locus_tag="WIC03_001548"
     CDS             complement(699..1316)
                     /locus_tag="WIC03_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEZ3884026.1"
                     /translation="MFTGIVQGLGKLTAIHRPSEAFHTYVVELPQEAADNLQRGASVA
                     NNGCCLTITEIEGNRVSFDLMAETLAKTNLGLLKEGDCVNIERAARFGDEIGGHVMSG
                     HIMATVPIVEIERDGFNRTVWFSLPHELKPYILTKGFVGLDGCSLTIGKVEDGRFNVH
                     LIPETLERTLFGSRKVGDRINIEIDPNTQAIVDTVERLMAQRYAK"
     gene            complement(1479..2093)
                     /locus_tag="WIC03_001549"
     CDS             complement(1479..2093)
                     /locus_tag="WIC03_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HEZ3884027.1"
                     /translation="MKTSNFSKRTSAMKTFALILADGQASRMGGEDKGLALLGGKALI
                     DHVIDRVRPQVSHIAISTNRNLEEYARRSPHIFPDARQWQHFGPLSALCTAANDLQLA
                     TADWLLVVPCDMPYLPGDLVARFETVSKRTPLCNAYYVETPITMHYNIMYIRPQILQS
                     AIPYLFSGMKTLRSWLQQQRARSVRFEFDGHFADLNTQIDLQEG"
     gene            2299..3638
                     /locus_tag="WIC03_001550"
                     /pseudo
     CDS             2299..3638
                     /locus_tag="WIC03_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229508.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili methyl-accepting chemotaxis
                     transducer N-terminal domain-containing protein"
     gene            3635..4291
                     /locus_tag="WIC03_001551"
     CDS             3635..4291
                     /locus_tag="WIC03_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEZ3884028.1"
                     /translation="MTIKIILIDDHTLFRSGIKALLSRQHGFEVIGEAADGLSGIKMI
                     SRLQPDVVLLDLDMPGMNGREALSQIISINPQQAVIMLTVSEDSDDLTECIRIGARGY
                     LLKNINADFLLESIRKAAEGDNVFSPEMTAKLVKSLISPQSTQGTQALSSLTPRELEI
                     LGYLAAGHSNKIIARHLDLAESTVKVHVQNLLRKLNLSSRVQAAVYAIRHNVPQPVPE
                     "
BASE COUNT         1135 a         1279 c         1081 g         1129 t
ORIGIN      
        1 cgttttgatc gaacggataa aaactgagcc acaatccgaa tcggtcggac agggatattt
       61 tttcttccac cgcttctttc tgatggattt ccccccgcat ccccgtcgta ccggcattct
      121 cgtcaaaata ttcgggcatc aggtgccgtc tgttggaagt cgcgtaaacc aaaacgttgg
      181 cgcaacgttg agacagaccg ccgtctaacg cggttttcaa tgccttatag gtttcatcgc
      241 cgctttcaaa cgacaaatcg tcgcaaaata cgataaattt ttccggacgt tccttcaaaa
      301 gcgtcaacag gtaaggcagg ccgattaaat cgcttttatc gacttcgatc aggcgcaatc
      361 ccttatccgc atattcgtgt agcagggctt tgaccagcga ggatttgcct gttccgcgcg
      421 cgccgctcat caatacattg ttcgcgggtc tgccgacaat gaactgttcg gtattacgca
      481 ccagcaattc ggtttgcctg ccgactcccg ccagccttac caagggaaag gtgtgcggat
      541 cgggcaagtg ttccaaaaaa ccttttttgc ccgcactctg ccagcggaag gcaagcgcgt
      601 tccaatccgt atgcccgggt tcgggcggaa gcacggcatc caaacgccgc aaaacggcat
      661 aggctttatc gagaaattcg ttcaattcca tctctgcctc actttgcata tctttgcgcc
      721 atcagccgtt cgacggtatc gacgattgcc tgcgtattcg gatcgatttc gatgttgatc
      781 ctgtcgccga cctttctgct gccgaacagc gtccgttcca aagtttcggg aatcagatgg
      841 acattgaaac ggccgtcttc gactttgcct atggtcaggc tgcaaccgtc caagccgacg
      901 aaccctttgg tcaggatata gggtttgagt tcatgcggga gcgaaaacca aaccgtgcgg
      961 ttgaacccgt cccgttcgat ttcgacaata ggcacggttg ccataatgtg tccgctcatg
     1021 acgtgtccgc cgatttcgtc tccgaaacgc gccgcccgtt cgatgttgac gcaatcgcct
     1081 tccttcagca gccccaaatt ggtttttgcc aaagtttccg ccattaaatc gaaactgacg
     1141 cggtttcctt cgatttcggt aatcgtcagg cagcagccgt tattggcgac cgatgcgccg
     1201 cgttgcagat tgtccgccgc ctcttgcgga agctcgacga cataagtgtg aaatgcctcc
     1261 gacgggcggt ggattgccgt cagttttccc aatccttgaa caatgcctgt aaacataatc
     1321 ctgtttccct gtgtcggtaa aaatggtgca aattgtagca tctccccgcg aaaaatgccg
     1381 tctgaaatgc cttcagacag cattatgcct ccgattcggg caaaaaccgc ccggtatggc
     1441 ttgacctttc ctttccacgc cggtcggcgg tcttgccctt atccctcctg caaatcgatt
     1501 tgcgtgttca agtcggcaaa atgcccgtca aactcgaatc tgaccgaccg cgccctttgc
     1561 tgctgcaacc agcttcttaa tgttttcata cccgaaaaca gatagggaat cgcgctttgc
     1621 agaatctgcg ggcggatata cataatgttg tagtgcatcg ttatcggcgt ttccacataa
     1681 tacgcattgc acaacggtgt gcgtttcgac acggtttcaa acctcgccac caaatcgccc
     1741 ggcagatacg gcatatcgca cggcacaacc aaaagccagt cggcagtcgc caactgcaaa
     1801 tcgttggctg cggtacacaa tgccgaaagc gggccgaaat gctgccactg ccgtgcgtcc
     1861 ggaaaaatat gcggacttct tcgagcatat tcttccaaat tccggttggt gctgatggcg
     1921 atatggctga cctgcggcct gaccctgtcg atgacatgat ctatcagtgc cttacccccc
     1981 aaaagagcaa gccccttgtc ctcgcctccc atacggctcg cctgaccgtc ggccagtatc
     2041 agggcaaaag ttttcattgc ggatgttctc ttggaaaagt tcgaggtttt catgattgca
     2101 gtctgccgtt cccaatgact caaaatgccg tctgaagcag acggcaaata aattcatatt
     2161 atctgaattt tatcataaca tgatttaaca ctgaagcggt acggattatg ttttccttaa
     2221 ccaattcttt ctgagcagtt gtatctaatc ccaaagaatg atattgtttg cattatttag
     2281 aacaattttt cgccgagcat gatactgcca gcccgttttt cagacggcat cagcctttcc
     2341 ctacgcctga aactcctgac cggagtgtgg gtcgggttgg cggcattgtc tgtcgttttg
     2401 acactgctgc tctctttgcg tctggaaaac gcggcctccg tcatcgaaga ggcgggcaac
     2461 ttgagaatgc aggcataccg tctggcatac atggcgggtg aaggatctcc ccgctcccaa
     2521 atagacaatc aggttgacga atttgaaaaa agcctgaatc gtatttcaca aagtgatgcc
     2581 atccatccgc tgattccttc ggacacccct cttgcttatg atttgataca atccatgctg
     2641 attatagatt ggcaggcaca catcctcccc ccgctccagt cctaccggcg accgactcag
     2701 gtcgatctct accgctttgc cggaaacatc gaactgtttt tgcaggcatt ggaaaatgcc
     2761 aacgaaaaaa acacatggtg gctcaggcgt tttcaatggg caattatgtt gatgacgctg
     2821 gtgtcgtctg tactgatgct gttttggcac cagatttggg ttatccggcc gctgcaggcg
     2881 ttaagggaag gtgcggaacg catcggacgg aggtgtttcg atattccggt tcccgaaggc
     2941 ggtacgccgg aattcaaaca ggtcgggcgt tgtttcaatc aaatgggcgg caggttgaaa
     3001 attttatatg atgatttgga aggacaagtc gccgagcaga cacgcagtct cgaaaaacaa
     3061 aatcaaaacc tgaccctgct gtaccaaact acacgggacc tgcaccaatc ctacataccg
     3121 caacaggctg cagaacattt tctaaaccgt atcctgcccg ccgtaggagc agattccggc
     3181 agagtttgtt tggacggcgg atccgatgtt tatgtttcca ttcatcatgc ggattgcggc
     3241 acagcagctt cggatttggg gaagtaccat gaggaaatct tcccgggata acggtcgaaa
     3301 ccgcctggga aaacggttcg ttcctgccgc ctcaggaagc gcagctccaa atgattttta
     3361 tcctgcagga aagcctgtcc aacatccgca aacacgcccg cgccacccat gtaaaattca
     3421 ccctttccga acacggcgga cgctttacca tgaccatcca agacaacgga caaggtttcg
     3481 acacggagaa aataggagaa cctacgggca gccatgtcgg actgcacatc atgcaggagc
     3541 gtgccaagcg catccatgcc gttttagaaa tccgttccca accccaacag ggaaccaccg
     3601 tctcattgac gggtgcatct gaagaaagct tgaaatgacc attaaaatta ttctgataga
     3661 cgaccacacc ctcttccgca gcggcattaa agcccttttg tcgcgccaac acggttttga
     3721 agtcatcggc gaagccgcag acggcctctc gggcatcaaa atgattagtc ggctgcaacc
     3781 cgatgtcgtc ctgctcgatt tggatatgcc cggcatgaac ggacgcgaag cactctccca
     3841 aatcatcagc atcaatccgc agcaggcagt gattatgctg accgtttccg aagacagcga
     3901 cgacttgacc gaatgtattc gcatcggcgc gcgcggttac ctgctgaaaa acatcaacgc
     3961 tgactttctg ctcgaaagca tacgcaaagc cgccgaaggc gataatgtat tctcgcccga
     4021 gatgaccgcc aaactcgtta aaagcctgat ttctccccag tccacacaag ggacgcaggc
     4081 actctcctca cttacccctc gtgaactgga aatcttgggc tatctcgccg caggacacag
     4141 caacaaaatc atcgcccgcc acctcgatct tgccgaatcc accgtcaaag tccacgttca
     4201 aaacctgctc cgcaaactca acctcagcag ccgggtgcag gccgccgttt acgccatccg
     4261 gcacaacgtc ccccaacctg tgccggaata aggcgggttc agacggcata ttaggggttt
     4321 taatccccgt acggtcattc ggataacaga ccaagcatgt aagtttatgc ccccataagt
     4381 acgcttggca tagcagtaat attgttcggt ttagtgtttt ccgtttgccc ctatctgata
     4441 ctgcaatatc aactatgccg tctgaaaacg catcatcatg atattttcag acggcataat
     4501 aaaaagcgga aatactaatg cagggtaaaa tgttccattc cgaatcccat aaatatacaa
     4561 tggcttatat cgtttaagca tgtgtaaccc accctcatat caatcaatat atagtggatt
     4621 aaca
//
LOCUS       DAWHSK010000158         4608 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.158, whole genome shotgun sequence.
ACCESSION   DAWHSK010000158 DAWHSK010000000
VERSION     DAWHSK010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4608
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.158"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..869
                     /locus_tag="WIC03_001552"
     CDS             <1..869
                     /locus_tag="WIC03_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HEZ3884029.1"
                     /translation="RNERGDRAAERANEAEIQSRNVQPAATVADAAPSETEMQTGKRR
                     RNGSRSERSQTAPETAAVAETAVQTAENTPSEPHTAEDKGSKPKSERNRRERDSRDAK
                     ERRERNNQRDRRQNGKKRNIPSAAKIEQYLNIHDTADKVRSAAAHVFGETDANAPITV
                     SIADPVAERDLPTASPAVSNGDAPVYDAAEKIRRATAAILPEGATPKAEAQEMPSETA
                     TFTAAAEQARETAQTGGLVLIETDPAALKAWAAQPEVQTGRGLRRSEQPKPSEVATVP
                     AEEMIQVETRQG"
     gene            complement(1819..2808)
                     /gene="pdxA"
                     /locus_tag="WIC03_001553"
     CDS             complement(1819..2808)
                     /gene="pdxA"
                     /locus_tag="WIC03_001553"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEZ3884030.1"
                     /translation="MKQPVFAVTSGEPAGIGPDICLDLAFARLPCRCAVLGDKNLLRA
                     RAEALGKSVVLRDFDPESGGAAYGELEVLHIPAVEAVEAGKLNPANAAYVLQLLDTAL
                     AGISDGIFDGIVTAPLHKGIINDARASTGFFSGHTEYLAEKSGTGQVVMMLAGKGLRV
                     ALVTTHLPLKDVAAAITQPLIESVARILHHDLKHKFGIKNPKILVAGLNPHAGEGGHL
                     GHEETDTIIPALENLRREGINLAGPYPADTLFQPFMLEGADAVLAMYHDQGLPVLKYH
                     SFGQGVNITLGLPFIRTSVDHGTALDLAATGRADSGSLITAVETAVEMARGSL"
     gene            2968..4395
                     /locus_tag="WIC03_001554"
     CDS             2968..4395
                     /locus_tag="WIC03_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEZ3884031.1"
                     /translation="MNENFTEWLHGWVGAINDPMWSYLVYMLLGTGLFFTVTTGFVQF
                     RLFGRSIKEMLGGRKQGDDPHGITPFQAFVTGLASRVGVGNIAGVAIAIKVGGPGAVF
                     WMWVTALIGMSSAFVESSLAQLFKVRDYDNHHFRGGPAYYITQGLGQKWLGVLFALSL
                     IFCFGFVFEAVQTNTIADTVKAAWGWEPHYVGVALVILTAPIIFGGIRRISKAAEIVV
                     PLMAVLYLFIALFIILTNIPMIPDVFGQIFSGAFKFDAAAGGLLGGLISQTMMMGIKR
                     GLYSNEAGMGSAPNAAAAAEVKHPVSQGMIQMLGVFVDTIIVCSCTAFIILIYQQPYG
                     DLSGAALTQAAIVSQVGQWGAGFLAVILFMFAFSTVIGNYAYAESNVQFIKSHWLITA
                     VFRMLVLAWVYFGAVANVPLVWDMADMAMGIMAWINLVAILLLSPLAFMLLRDYTAKL
                     KMGKDPEFKLSEHPGLKRRIKSDVW"
BASE COUNT         1045 a         1336 c         1237 g          990 t
ORIGIN      
        1 gccgcaatga acgcggcgac cgtgccgccg aacgcgccaa cgaagcggaa atccaaagcc
       61 gcaacgtaca gcctgccgca accgttgcag atgccgcacc gtccgaaacc gaaatgcaaa
      121 ccggcaaacg ccgccgcaac ggcagccgca gcgaacgcag ccaaaccgcg ccggaaaccg
      181 ccgccgttgc cgaaacagcc gttcagacag cggaaaacac gccgtccgaa ccgcataccg
      241 cagaagacaa aggcagcaag cccaaatccg aacgcaaccg ccgcgaacgc gacagccgcg
      301 acgccaaaga acgccgcgag cgcaacaatc agcgcgaccg ccgtcaaaac ggcaaaaaac
      361 gcaatatccc gtctgccgcc aaaatcgagc agtacctgaa tattcacgac accgccgaca
      421 aagtccgttc cgccgccgcg cacgttttcg gcgaaaccga cgcaaacgcg ccgattaccg
      481 tcagcattgc cgatccggtt gcagaaaggg atcttccgac agcatctccc gccgtttcaa
      541 acggcgacgc accggtttat gatgcggcgg aaaaaatccg ccgtgccacc gccgccatcc
      601 tgcccgaagg cgcgacaccg aaagccgaag cacaggaaat gccgtctgaa accgcaacct
      661 ttacggctgc ggcggaacag gcacgggaaa ccgcacaaac cggcggactc gtcctgatcg
      721 aaaccgaccc tgccgcattg aaggcatggg cggcacaacc cgaagtccaa accggacgcg
      781 gtttgcgccg ttccgaacag cccaagccgt ctgaagtcgc aaccgtccct gccgaagaaa
      841 tgatccaagt cgaaacccgg caaggctgaa ccgacggcgg caaaaagagg ttctgttccg
      901 cagaacctct tttttatata gattcggata cctgcaatgc cgtctgaagc ctcaccattc
      961 ccatgattac cgcaaccttt cgtcattccc gtgaaaacgg gaatctagaa cctcaaactt
     1021 tcaaataacc tttgaatatt gccgctgcct taaggtctgg attcccacct gcgcggaatg
     1081 acggtttaga agttgcccga aacctcaaaa aaaactgaaa ccgaacaagc cggattcccg
     1141 caacattccg tcattcccgt gaaaacggga atatagaacc ccaaactttc agataatctt
     1201 tgaatattgc cgtcgcccaa aggcctggat tcccgcctgc gcgggaatga cggcggaggg
     1261 tggacgatgc cgtctgaaac ttcgccattc ccatgattac cgcaaccttt cgtcattccc
     1321 gccacctttc gtcattcccg tgaaaacggg aatatagaac ctcaaacttt cagataatct
     1381 ttgaatattg ccgccgccca aaggtctgga ttcccgcctg cgcgggaatg acggcggagg
     1441 gtgaacgatg ccgtctgaaa cttcaccatt cccatgatta ccgcaacctt tcgtcattcc
     1501 cgtgaaaacg ggaatctaga acctctaaac tttcagataa tctttgaata ttgccgtcgc
     1561 ccaaaggcct ggattcccgc ctgcgcggga atgacggttt agaagttgcc cgaaacctca
     1621 aaaaaaaacc gaaaccgaac aagccggatt cccgcaacat tccgtcattc ccgtgaaaac
     1681 gggaatctag aacctcaaac tttcagataa tctttgaata ttgccgccgc ccgaaggtct
     1741 ggattcccgc ctgcgcggga atgacggcgg agggtggacg atgccgcctg cgcggaaatg
     1801 acgggtcttt tatcatcttt aaaggctgcc gcgcgccatc tcgacggcgg tctccacggc
     1861 agttatcagg ctgccggaat ccgccctgcc ggttgccgcc aaatcgagcg cggtgccgtg
     1921 atcgacagag gtgcggataa agggcagacc cagcgtgatg ttcacgccct gcccgaaact
     1981 gtggtatttc aacacgggca gcccttggtc gtggtacatc gccaatacgg catccgcacc
     2041 ttcgagcata aacggctgga acaatgtgtc cgccggatac gggccggcaa ggtttatccc
     2101 ttcgcggcgc aggttttcca atgcagggat aatggtgtcg gtttcttcgt gtccgaggtg
     2161 tccgccttcg ccggcgtggg gattaagtcc ggcgacaagg attttgggat ttttgatgcc
     2221 gaatttgtgt tttaagtcgt gatgcaaaat gcgtgcgacg ctttcaatca gcggttgcgt
     2281 gatggcggcg gcaacgtctt tcagcggcag gtgggtcgtt acgagggcga cgcgcaggcc
     2341 tttgccggca agcatcatca cgacctgccc cgtgccgctt ttttccgcca gatattcggt
     2401 gtgtccgctg aaaaaacctg tgcttgcgcg cgcgtcgttg atgatgcctt tgtgcagcgg
     2461 cgcggtaacg atgccgtcga aaatgccgtc tgaaatgcct gcgagcgcgg tgtccaaaag
     2521 ttgcagcaca taggcggcgt tggcgggatt gagtttgccc gcctcaaccg cttcgacggc
     2581 agggatgtgc agcacttcca gctcgccgta tgccgcgccg cctgattctg gatcgaagtc
     2641 gcgcaggacg acgcttttgc ccaaggcttc ggcgcgcgcg cgcaataggt ttttgtcgcc
     2701 caataccgcg cagcggcagg gcaggcgtgc aaacgccaag tccaaacaaa tatcggggcc
     2761 gatgccggca ggctcgccgg aagtaacggc aaaaacaggc tgtttcatcg tgtttgctcc
     2821 aaacaaaatg cgattctaac gccgcagccg cgcggcgatg taaatttttc tgattttgtt
     2881 gacaatctgc tagaatgggc gtttacaaaa tttaaaccct gcttgcatac cgccaatatg
     2941 tgcgagtttc aactttaagg aagcgatatg aacgagaact ttaccgaatg gctgcacggc
     3001 tgggtcggcg ccatcaacga tccgatgtgg tcatacttgg tttatatgct tttgggtacg
     3061 gggcttttct tcaccgtaac cacgggcttt gtccaattcc gcctgttcgg gcgcagcatc
     3121 aaagaaatgc tcggcggccg caaacagggg gacgaccctc acggcatcac gccgtttcag
     3181 gcatttgtaa ccggccttgc cagccgcgtg ggcgtgggca atatcgcggg cgtggccatc
     3241 gccatcaaag tcggcggacc gggcgcggtg ttttggatgt gggtaaccgc cttaatcggt
     3301 atgagttcgg cgtttgtcga atcttcgctg gcgcagctct ttaaagtccg cgactacgac
     3361 aaccaccatt tccggggcgg ccctgcctac tacatcactc aagggctggg gcagaaatgg
     3421 ctgggcgtgt tgttcgccct gagcctgatt ttctgtttcg gctttgtgtt tgaagcggtt
     3481 cagaccaata ccattgccga taccgtcaaa gcggcgtggg gttgggagcc tcattatgtc
     3541 ggcgtcgccc tggtgatttt aaccgcgccg attatcttcg gcggcatcag gcgcatatct
     3601 aaagcggcgg aaatcgtcgt ccccctgatg gcggttttgt acctctttat cgcgcttttc
     3661 atcattttga ccaatattcc gatgattccg gacgtgttcg gtcagatttt ttcgggcgcg
     3721 ttcaaattcg acgcggcagc aggcggctta ctcggcggtc tgatttcgca aacgatgatg
     3781 atgggcatca aacgcggcct gtattccaac gaggcgggta tgggttccgc gccgaacgcc
     3841 gccgccgccg ccgaagtgaa acaccctgtt tcgcaaggta tgattcaaat gctgggcgtg
     3901 tttgtcgata ccatcatcgt ttgttcttgc accgccttca tcatcttgat ttaccaacag
     3961 ccttacggcg atttgagcgg tgcggcgctg acgcaggcgg cgattgtcag ccaagtgggg
     4021 caatggggcg cgggcttcct cgccgtcatc ctgtttatgt ttgccttttc caccgttatc
     4081 ggcaactatg cctatgccga gtccaacgtc caattcatca aaagccattg gctgattacc
     4141 gccgttttcc gtatgctggt tttggcgtgg gtctatttcg gcgcggttgc caatgtgcct
     4201 ttggtctggg atatggcgga tatggcgatg ggcattatgg cgtggatcaa ccttgtcgcc
     4261 atcctgctgc tctcgccact ggcgtttatg ctgctgcgcg attacaccgc caagctgaaa
     4321 atgggcaaag accccgagtt caaactttcc gaacatccgg gcctgaaacg ccgtatcaaa
     4381 tccgacgttt ggtaaatccc gcccttaccg gagccgcttc cccgcgaagc ggcttttccc
     4441 tttccgcaca ctgtaaaaac agggcgaaca agcgtacaat cccaaccctt tacttttgaa
     4501 tccatttcgt ttttcagacg gcatattgaa tatagtggtt taacaaaaat cgggacaagg
     4561 cggcgaagcc gcagacagta cagatagtac agaaccgatt cacttggt
//
LOCUS       DAWHSK010000159         4571 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.159, whole genome shotgun sequence.
ACCESSION   DAWHSK010000159 DAWHSK010000000
VERSION     DAWHSK010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4571
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.159"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(322..567)
                     /locus_tag="WIC03_001555"
     CDS             complement(322..567)
                     /locus_tag="WIC03_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884032.1"
                     /translation="MLKSIELNSHIRNRLAEYLKGRGMDFQTAMQEEKGNKEIAAIVH
                     SGLPTLVRKLYSEQKMQKFFWEKRDLIADYISRRMQG"
     gene            679..1107
                     /locus_tag="WIC03_001556"
     CDS             679..1107
                     /locus_tag="WIC03_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEZ3884033.1"
                     /translation="MMFSWFKLFHLFFVISWFAGLFYLPRIFVNMAMIDVPRGNPEYV
                     RLSGMAVRLYRFMSPLGFGAVVFGAAIPFAAGWWGSGWVHVKLCLGLMLLAYQLYCGV
                     LLRRFQDYSNAFSHRWYRVFNEIPVLLMVAALYLVVFKPF"
     gene            1119..1595
                     /locus_tag="WIC03_001557"
     CDS             1119..1595
                     /locus_tag="WIC03_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEZ3884034.1"
                     /translation="MPIEIERRFLIGNDNWRQYADEPLLLRQGYLSVEKERTVRVRIA
                     GKQAWLTLKGYISEISRSEFEYEIPLADAEKMMETMCPFKMEKWRYPVRWGGSLFEID
                     VFLGDNAPLVVAEIELPDENADFDRPDWLGREITADGMFTNAYLSRHPFSSWKNAV"
     gene            complement(1945..2433)
                     /locus_tag="WIC03_001558"
     CDS             complement(1945..2433)
                     /locus_tag="WIC03_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEZ3884035.1"
                     /translation="MLKITLIAACAENLCIGAGNAMPWHIPEDFAFFKAYTLGKPVIM
                     GRKTWESLPVKPLPGRRNIVISRQADYCAAGAETAASLEAALALCAGAEEAVIMGGAQ
                     IYGQAMPLATDLRITEVDLSVEGDAFFPAIDRTHWKEAERTERRVSSKGTRYAFVHYL
                     RY"
     gene            complement(2501..3556)
                     /gene="aroG"
                     /locus_tag="WIC03_001559"
     CDS             complement(2501..3556)
                     /gene="aroG"
                     /locus_tag="WIC03_001559"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HEZ3884036.1"
                     /translation="MTHHYPTDDIKIKEVKELLPPIAHLYELPISKEASGLVHRTRQE
                     ISDLVHGRDKRLLVIIGPCSIHDPKAALEYAERLLKLRKQYENELLIVMRVYFEKPRT
                     TVGWKGLINDPHLDGTFDINFGLRQARSLLLSLNNMGMPASTEFLDMITPQYYADLIS
                     WGAIGARTTESQVHRELASGLSCPVGFKNGTDGNLKIAIDAIGAASHSHHFLSVTKAG
                     HSAIVHTGGNPDCHVILRGGKEPNYDAGHVSEAAEQLRAAGVTDKLMIDCSHANSRKD
                     YTRQMEVAQDIAAQLEQDGSNIMGVMVESHLVEGRQDKPEVYGKSITDACIGWGATEE
                     LLALLAGANKKRMARAG"
     gene            complement(3718..3945)
                     /locus_tag="WIC03_001560"
     CDS             complement(3718..3945)
                     /locus_tag="WIC03_001560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884037.1"
                     /translation="MGNTRIPIKSFFKQLVLFVFNIFLAYEILPDVQAESAAGCPVPD
                     CRGNGKTDVPCGFVRMNGGYCFYQSVSFSLE"
     gene            3881..4113
                     /locus_tag="WIC03_001561"
                     /pseudo
     CDS             3881..4113
                     /locus_tag="WIC03_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212266.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4172..4480
                     /locus_tag="WIC03_001562"
     CDS             4172..4480
                     /locus_tag="WIC03_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884038.1"
                     /translation="MTPWADAYADLRGKTKVMTTQMGASRDVSKSAKGWSVGIGLNVG
                     KQLTDSVGLEFDPYYRHKTIYKPREIVLDGDKTKMGRSKSNEYGFRVAATFYSQLKSK
                     "
BASE COUNT         1117 a         1183 c         1065 g         1206 t
ORIGIN      
        1 aaaccctccg ccgtcattcc cgcgaaagcg ggaatctaga attttaatgc ggcaaaaatt
       61 tatcggaaaa aaccgaagtt taaagaccta gattcccgcc tgcgcgggaa tgacggttta
      121 gaaatcaccc gaaacgagaa aaagaaccga aaccgaacaa gccggattac cgcctgcgcg
      181 ggaatgacgg ctcaaaagtt acgaaacgaa aaataaccga aaccaaacaa gtcggattcc
      241 cgcgcgggcg ggaatgacga taagcgtaat ttcaatctgc catattgtct tttcacttgc
      301 ctgaaaacag atttcagcca cctatccctg catccggcgg ctgatgtagt cggcaatcaa
      361 atcccgcttt tcccaaaaaa acttctgcat tttttgttcg gaatacagtt tgcggaccag
      421 agtgggcaaa ccgctgtgga cgatggcggc gatttctttg ttgccttttt cttcctgcat
      481 tgccgtctga aaatccatac ccctgccttt cagatattct gcaaggcggt tgcggatgtg
      541 ggaattgagt tcgatggatt tgagcatttt gcctccgttt tgcggtaagg atttattcga
      601 tttgtttatc cgcctgattt tatcacgttt cccaagcggt ttgcctgtac aataccgctt
      661 ttcggcaggg tgtgggcgat gatgttttct tggttcaagc tgtttcactt gttttttgtc
      721 atttcgtggt ttgcagggct gttttacctg ccgaggattt tcgtcaatat ggcgatgatt
      781 gatgtgccgc gcggcaatcc cgagtatgtg cgtctgtcgg gcatggcggt gcggctgtac
      841 cgttttatgt cgccgttggg cttcggcgcg gtcgtgttcg gcgcggcgat accgtttgcc
      901 gccggctggt ggggcagcgg ctgggtacac gtcaaactgt gtttgggctt gatgctcttg
      961 gcttaccagt tgtattgcgg cgtgctgctg cgccgttttc aggattacag caatgctttt
     1021 tcacaccgct ggtaccgcgt gttcaacgaa atccccgtgc tgctgatggt tgccgcgctg
     1081 tatctggtcg tgttcaaacc gttttgacgg agcatcttat gccgatagaa atcgaacgcc
     1141 gttttttgat tggaaacgac aactggcggc aatacgccga cgaaccgctg ctgttgaggc
     1201 agggttatct gtctgttgaa aaagaacgca ccgtccgtgt ccgcattgcc ggaaaacagg
     1261 cgtggctgac gctgaaaggc tatatttcgg aaatcagccg cagtgagttt gaatacgaaa
     1321 tcccgcttgc cgatgcggaa aagatgatgg aaacgatgtg cccgtttaaa atggaaaaat
     1381 ggcgttatcc ggtcagatgg ggcggcagcc tgtttgaaat cgatgttttt cttggcgata
     1441 atgcgccttt ggtcgtcgcg gaaatcgagt tgcccgacga gaatgccgat tttgaccgtc
     1501 cggactggct gggacgggaa atcactgccg acggtatgtt taccaatgcc tatttgagca
     1561 ggcatccgtt ctcaagctgg aaaaatgccg tctgaaacgg cttcagacgg cattctccga
     1621 caaccatcgc aaatccaaag acagcctcta tatccttatt tctgctttct ttgttccatt
     1681 attcccaata aatccccata acctaaaatc tcgtcattcc cgcgcaggcg ggaatccggt
     1741 tctttaaact tcggtttttt ccgataaatt gctttagctt ttcgtttccg gattcccgct
     1801 tttgcgggaa tgacgatgga agattgttgt tatttcggat aaattaccct aactttaaaa
     1861 tcccggattc ccatcttcgg gggaataaca atattttcta tttcagccca tcatttgcgg
     1921 gggattttat agagagttta tatttcaata tctcaaataa tgcacaaaag catagcgcgt
     1981 gcctttgctg ctgacacggc gttccgtccg ctctgcttct ttccaatgcg tccggtctat
     2041 tgcggggaaa aatgcatctc cttccacaga caaatccact tcggttatcc gcaaatcggt
     2101 cgccaatggc atcgcttgtc cgtatatctg cgcgccgccc ataatgacgg cttcttccgc
     2161 gcctgcgcac aatgccaatg ccgcctccaa acttgccgcc gtttccgcgc ctgccgcgca
     2221 ataatccgcc tgccggctga tgacgatgtt cctccgtccg ggcaggggtt tgacgggcag
     2281 ggattcccac gttttccgcc ccataatgac gggtttgccc aaggtatagg ctttgaaaaa
     2341 tgcgaaatct tcggggatgt gccaaggcat agcattgccc gccccgatgc acaggttttc
     2401 cgcacacgcc gcaattaggg ttattttgag catatttcct ccacaaaaat gccgtctgaa
     2461 cgcttcagac ggcattttat gacattccgt gtcaaaaatc tcaaccggcg cgcgccatac
     2521 gttttttgtt tgcacctgcc aacaatgcca acagttcttc agtcgcgccc caaccgatac
     2581 acgcatcggt aatgcttttg ccgtacactt ccggcttatc ctgtctgcct tcgaccaaat
     2641 ggctttccac catcacgccc ataatattgc tcccgtcctg ttccaattgg gcagcaatat
     2701 cttgtgccac ttccatctgc cgggtgtaat ccttgcggct gttggcgtgg ctgcaatcta
     2761 tcatcagctt atcggttacc cctgccgcac gcagttgttc cgccgcctcg ctgacgtgtc
     2821 ccgcatcata attcggctct tttccgccgc gcaaaatgac atgacagtcg ggattgccgc
     2881 cggtatggac aatggcggaa tgtccggcct tggttacaga caagaaatga tgcgaatggc
     2941 tcgccgcacc gattgcgtcg atggcaatct tcaaattgcc atccgtaccg tttttaaagc
     3001 cgacggggca ggacagcccg cttgccaatt cgcggtgaac ttggctttcg gtcgtccgcg
     3061 caccgattgc cccccaagaa atcaagtccg cataatattg cggcgtaatc atatccaaaa
     3121 actcggtaga ggcaggcata cccatattgt tcagcgacaa caacaggctg cgcgcctgac
     3181 gcaaaccgaa attgatatcg aacgtaccgt ccaaatgcgg gtcgttaatc aaacctttcc
     3241 aacccaccgt cgtcctcggc ttctcgaaat aaacgcgcat cacaatcaaa agctcgtttt
     3301 catactgctt gcggagtttc aacaaacgct ccgcatattc caacgccgct ttcggatcgt
     3361 gaatcgaaca cggcccgata ataaccaaca gccgcttgtc cctgccgtga accaaatcgg
     3421 aaatttcctg acgggtgcgg tgaaccaagc ccgaagcctc tttggaaatc ggcagctcgt
     3481 aaagatgggc aatcggcggc aacaactctt taacttcttt aatcttaata tcgtctgtgg
     3541 ggtaatggtg tgtcatcttg catctttcct gatggttttt tatacgcggc ttaggttaca
     3601 tcgtttacct tgcacaaaca agtgcaacat attactacaa agctatttta tttcaaaata
     3661 actgatatat cttttttcat agattttaaa tttcatattt ttaatatttt aggaatttta
     3721 ttccaaagaa aaagaaacag attgataaaa acaatacccg ccattcattc ggacaaaacc
     3781 gcacggaaca tccgttttcc cgtttccacg acaatccggt acaggacacc ccgctgccga
     3841 ttccgcctgc acatccggca aaatttcata cgcaaggaaa atgttgaaaa caaaaagaac
     3901 taattgcttg aaaaaagatt taattggaat gcgggtattt cccacacttt ctccatttat
     3961 tcatcctcat tataggaaga aaaacatgaa aaaaccctgc ttgcactctc acttgcagcc
     4021 gtgtccgccg ttacggtagc cgatattccc gagtctaacg gcaagggcaa agccatcatc
     4081 ggcgataagg aaaaacaccg ttaccaagca tgaaaacctt gctttgaaag tccatggcga
     4141 aatccgccaa gatttggggg acggatgatc cttgacccct tgggcggatg catatgcaga
     4201 tttgcgcggc aaaaccaaag tgatgacgac ccagatgggt gcttcccgcg atgtcagcaa
     4261 aagcgccaaa ggttggagtg tcggtatcgg tctgaatgta ggcaaacagt tgaccgacag
     4321 cgtcggtctc gagtttgatc catactaccg tcacaaaaca atctacaaac cccgtgagat
     4381 tgtcttggac ggtgacaaaa ccaaaatggg ccgctccaaa tccaacgagt acggcttccg
     4441 cgtagccgca acgttctata gtcaattaaa atcaaaatag gacagtagtg catcgtcaaa
     4501 tcgggcgtaa tcagacaaaa cggttcgcag ataccgctta atattcgccc acactttctc
     4561 aatcgggttg a
//
LOCUS       DAWHSK010000160         4557 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.160, whole genome shotgun sequence.
ACCESSION   DAWHSK010000160 DAWHSK010000000
VERSION     DAWHSK010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4557
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.160"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(245..352)
                     /locus_tag="WIC03_001563"
                     /pseudo
     CDS             complement(245..352)
                     /locus_tag="WIC03_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(398..1126)
                     /gene="pdxJ"
                     /locus_tag="WIC03_001564"
     CDS             complement(398..1126)
                     /gene="pdxJ"
                     /locus_tag="WIC03_001564"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEZ3884039.1"
                     /translation="MLLGVNIDHIATVRNARGTIYPSPVEAALIAETHGADLITMHLR
                     EDRRHIKDADVFAVKNAIRTRLNLEMALTEEMLENALKVMPEDVCIVPEKRQEITTEG
                     GLDVLAQQDKIAEFTKILTDAGIRVSLFIDADDRQIQAARDVGAPVVELHTGAYADAR
                     SHAEQLKQFERLQNGAHFAGDLGLVVNAGHGLTIHNVTPIAQILAIRELNIGHSLIAQ
                     ALFLGLPEAVRQMKEAMFRARLLP"
     gene            complement(1179..1922)
                     /gene="recO"
                     /locus_tag="WIC03_001565"
     CDS             complement(1179..1922)
                     /gene="recO"
                     /locus_tag="WIC03_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEZ3884040.1"
                     /translation="MSEHRVNHEPVFLLASSPWRESSLRVEAFSRRYGRVALLARSAR
                     KRQSELRGVLVPFVPVSASWYGSQELKTLHRAEWIGGWPQPQGRALFSGLYVNELVLK
                     LTAREDPMPELYDALAKVMEAVCREANHIADLRRFEWKLLNALGVAPDLHADGTGGDI
                     LADKTYRLMPEEAVMPVCEDADALSHEAGAIVEGQSLIDLREGSFRTAESLQQALKIT
                     RLFIRHLLPEGLKSRQVLEQIRQFDRNTA"
     gene            complement(1956..3083)
                     /gene="pheA"
                     /locus_tag="WIC03_001566"
     CDS             complement(1956..3083)
                     /gene="pheA"
                     /locus_tag="WIC03_001566"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEZ3884041.1"
                     /translation="MLECTINYCSGEIMSQTIDELLLPHRNAIDTIDAEILRLLNERA
                     QHAHAIGELKGTGAVYRPEREVAVLRRIQDLNKGPLPDESVARLFREVMSECLAVERP
                     LTIAYLGPQGTFTQQAAIKHFGHAAHTMACPTIDNCFKQVETRQADYLVAPVENSTEG
                     SVGRTLDLLAVTALQACGEVVLRIHHNLLRKNNGSTEGIAKVFSHAQALAQCNDWLGR
                     HLPNAERIAVSSNAEAARLVAESDDGTVAAIAGRTAAEIYGLDMVAECIEDEPNNTTR
                     FLVMGHHETGASGSDKTSLAVSAPNRAGAVASLLQPLTESGISMTKFESRPSKSVLWE
                     YLFFIDIEGHRRDAQIQTALERLGERASFVKVIGSYPTAVL"
     gene            complement(3122..4354)
                     /locus_tag="WIC03_001567"
     CDS             complement(3122..4354)
                     /locus_tag="WIC03_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HEZ3884042.1"
                     /translation="MPSAHYPEMSEKLMAVLMAMLVTLMPFSIDAYLPAIPEMAQSLN
                     ADVHRIEQSLSLFMFGTAFGQVVGGSVSDIKGRKPVALTGLAVYCLAVAAIVFASSAE
                     QLLNLRVVQAFGAGMTVVIVGAMVRDYYSGRKAAQMFALIGIILMVVPLVAPMVGALL
                     QGLGGWQAIFVFLAAYSLVLLGLVQYFLPKPAVGGKIGRDVFGLVAGRFKRVLKTRAA
                     MGYLFFQAFSFGSMFAFLTESSFVYQQLYRVTPHQYAWAFALNIITMMFFNRVTAWRL
                     KTGVHPQSILLWGIVVQFAANLSQLAAVLFFGLPPFWLLVACVMFSVGTQGLVGANTQ
                     ACFMSYFKEEGGSANAVLGVFQSLIGAGVGMAATFLHDGSATVMAATMTASTSCGIAL
                     LWLCSHRAWKENGQSEYL"
BASE COUNT         1031 a         1298 c         1236 g          992 t
ORIGIN      
        1 ctatattaca taatcatcgc ctcaatattt aatatcccaa aatatagatt taaaaccatc
       61 gcctttgtgg tttgtcagca ttcctaattg agatagaaag tcaaataatt tcttgtttct
      121 ccggctgtca tcaagatggt ttagattaag accgcgaaca atacaataac caatacgctg
      181 tactgtattg tgaatttctt ctatcaaact attattgttt gcagataaaa tatacttttg
      241 attcatatag tgaattaaat ttaaaccagt acggcgttgg cttgccttgc cgtactattt
      301 gtactgtctg cggcttcgcc gccttgtcct gatttaaatt taatccacta taacatatcc
      361 ctgtcgtgaa atgctgtctg aaagggtttg cctgccctta cggcagcagc cttgccctga
      421 acatcgcctc cttcatttgg cgcacggctt cgggcagtcc gaggaagagg gcttgggcaa
      481 tcagcgaatg cccgatgttc agttcgcgga tggcgaggat ttgggcgatg ggggtaacgt
      541 tgtgtatggt cagtccgtgt ccggcgttga cgaccaagcc caaatcgccg gcaaaatgcg
      601 cgccgttttg cagacgctcg aattgcttga gttgttcggc gtggctgcgc gcgtcggcat
      661 acgcgcctgt gtgcagctcg acaacgggcg caccgacatc acgggcggct tggatttgcc
      721 tgtcgtcggc atcgataaac aaggacacgc gtatgcccgc atcggtcagg attttggtga
      781 actcggcaat tttgtcttgt tgcgccaata cgtccaaacc gccttcggtc gtgatttcct
      841 gccgtttttc aggcacgatg cacacgtctt ccggcatcac tttaagcgcg ttttcgagca
      901 tttcttccgt caacgccatt tcaaggttca ggcgcgtgcg gatggcgttt ttaacggcaa
      961 acacgtccgc atctttaata tggcggcggt cttcacgcaa gtgcatggta atcaaatctg
     1021 cgccgtgggt ttccgcaatc agcgccgctt cgacagggct gggataaatc gtaccgcgtg
     1081 cattgcggac ggtggcaata tggtcgatgt tgacacctaa aagcatgatg tttcctttcc
     1141 tcttcgtttt tctgtttggc ggcatggtgc gggcgcagct acgcggtatt gcggtcaaac
     1201 tgccttatct gttccaacac ctgccgcgat ttcagccctt cgggcaacag gtggcggata
     1261 aaaagccgtg taattttcaa tgcctgttgc aggctttcgg cggtgcggaa actgccttcg
     1321 cgcaaatcga tcaggctttg cccttcgaca attgcgccgg cttcgtgcga aagcgcgtct
     1381 gcgtcctcgc atacgggcat gaccgcctct tcgggcatca gccggtaggt tttgtccgcc
     1441 aaaatatcgc cgcccgtgcc gtcggcgtgc aaatcggggg caacgcccaa ggcattgagc
     1501 agcttccact cgaagcggcg caaatcggca atatggttgg cttcacggca caccgcctcc
     1561 atcacttttg ccaacgcgtc gtacagttcg ggcatcggat cttcgcgcgc ggtcagtttc
     1621 aacaccagtt cgttcacata cagaccgctg aacaaagccc taccctgcgg ctgcggccac
     1681 ccgcctatcc attcggcgcg atgtagggtc ttcaactctt gcgaaccata ccaagacgca
     1741 ctcaccggca caaacggcac caacacgccg cgcaattcgc tctgccgctt gcgcgcgctt
     1801 cttgccaaaa gtgccacacg cccgtaacgg cggctgaagg cttcgacccg caggctgctt
     1861 tcgcgccatg gcgaggaagc caacagaaag acgggttcgt ggttgactcg gtgttcggac
     1921 atcatgcctg aaatgccgtc tgaacgctgc tgccgctaca aaacggcggt cgggtacgaa
     1981 ccgatgactt tgacgaacga agcgcgttcg cccaagcgtt ccaatgccgt ctgaatctgc
     2041 gcgtcccggc ggtgtccctc gatatcgata aagaacaggt attcccacaa aacggatttg
     2101 ctcggacggc tctcaaactt ggtcatggaa atacccgact cggtcagcgg ttgcagcagc
     2161 gaggcaaccg caccggcgcg gttgggcgcg gaaacggcca gcgaagtctt gtcgctgccg
     2221 cttgcaccgg tttcgtgatg tcccatcacc aagaagcgcg tggtgttgtt cggttcgtct
     2281 tcgatgcact cggcaaccat atcgagtccg tagatttccg ccgccgtgcg tccggcgatg
     2341 gcggcaaccg taccgtcgtc cgattcggca accagccttg cggcttcggc attgctggac
     2401 acggcaatcc gttcggcgtt gggcaggtgt ctgcccaacc agtcgttgca ctgcgccaac
     2461 gcctgcgcgt gggaaaagac tttggcaatg ccttcggtgc tgccgttgtt tttacgcaaa
     2521 aggttgtggt ggatgcgcaa aacgacttcg ccgcacgcct gcaacgcggt aacggcaagc
     2581 aaatccaacg tgcgcccgac cgagccttcg gtcgagtttt ccacgggcgc gaccagataa
     2641 tccgcctgac gggtttcaac ctgcttgaag cagttgtcta tggtcggaca cgccatggtg
     2701 tgcgcggcgt gtccgaaatg cttgatcgcc gcctgctggg taaacgtgcc ctgcggcccc
     2761 agataggcga tggtcagcgg gcgttctacg gcgaggcact cgctcatcac ttcccgaaac
     2821 aggcgtgcta ccgattcgtc gggcagcggg cctttgttca aatcctgaat gcggcgcaac
     2881 acggcgactt cgcgttcggg acggtacact gcgcccgtgc ctttcagttc gccgatggcg
     2941 tgtgcgtgtt gcgcgcgttc gttgagcagg cgcaggattt ccgcatcaat ggtgtcgatg
     3001 gcgttgcggt ggggaaggag gagttcgtcg atagtttggg acataatttc gcctgaacag
     3061 taatttatcg tgcattctaa catcaaatgc cgtctgaaac ggtttcagac ggcattttcc
     3121 gttaaaggta ttcgctttgc ccgttttctt tccacgcacg atgcgagcag agccacagaa
     3181 gcgcaatgcc gcaagaggtg gacgcggtca tcgttgccgc catcacggtt gccgaaccgt
     3241 cgtgcaagaa ggtcgccgcc atacccaccc ccgcgccgat taaagattgg aatacaccca
     3301 atacggcgtt tgcgctgccg ccctcttctt tgaaatagga cataaaacac gcctgcgtgt
     3361 ttgcaccgac caagccctgc gtaccgacgg aaaacatcac gcacgcgacc agcagccaaa
     3421 acgggggcaa cccgaaaaac agcacggcgg cgagttggga caggttggcg gcaaactgga
     3481 cgacaatccc ccatagcagg atgctttgcg gatgcacgcc ggttttgagc cgccacgcgg
     3541 taacgcggtt gaaaaacatc atcgtgatga tgtttagtgc aaacgcccaa gcgtattgat
     3601 gaggcgtaac acggtagagc tgctggtaca cgaaggaaga ttcggtcaga aaggcgaaca
     3661 tcgaaccgaa gctgaatgcc tgaaaaaaca gataacccat cgcggcacgg gttttcaata
     3721 cgcgcttgaa ccgccccgcc accagcccga acacgtcccg tccgattttg ccgccgacgg
     3781 cgggcttggg caggaaatac tgtaccaaac cgagcagcac cagcgaatac gccgccagaa
     3841 aaacaaaaat cgcctgccag ccacccaagc cctgcaacaa tgcgccgacc atgggtgcga
     3901 ccagcggcac aaccatcaaa atgatgccga taagggcaaa catctgggcg gctttgcgtc
     3961 cggaataata atcgcgcacc attgcgccga cgatgaccac agtcatgccc gcaccgaatg
     4021 cctgcacgac gcgcaggttg aggagctgtt cggcactcga agcaaatacg atggcggcaa
     4081 cggcaaggca gtagacggcc agtccggtca gcgcgacggg tttgcgccct ttgatgtcgg
     4141 acaccgaacc gccgaccacc tgtccgaacg ccgtgccgaa cataaacaaa ctcaaactct
     4201 gttcgatgcg gtgaacatcc gcgttcagcg attgcgccat ttcgggaatc gcgggcaggt
     4261 aggcatcgat ggaaaacggc atcagcgtaa ccagcatcgc catcaaaacc gccatcagtt
     4321 tttcgctcat ttcaggataa tgggcagaag gcataaattc tcgtccgatt cgcaaacaaa
     4381 ccgcccggcg gcggaaaaca caatacgcgg aaacggaaaa ttttaacaca ccggcacaaa
     4441 caggcggcaa caaaagcccc ggcaaaaccg gacgaaccgg aacgatatag tggattaaca
     4501 aaaaccagta cagcgttgcc ttgccttagc tcaaagagaa cgattctcta aggtgct
//
LOCUS       DAWHSK010000161         4513 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.161, whole genome shotgun sequence.
ACCESSION   DAWHSK010000161 DAWHSK010000000
VERSION     DAWHSK010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4513
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.161"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..485)
                     /locus_tag="WIC03_001568"
     CDS             complement(<1..485)
                     /locus_tag="WIC03_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEZ3884043.1"
                     /translation="MRTNYCGLISEQYLDQTVTVKGWVHRRRDHGGVIFIDLRDREGI
                     VQVVIDPDTPEAFAAADSSRNEYVLSITGRVRNRPEGTTNDKMISGKIEILAKEIEVL
                     NAAATPPFQIDDENISENVRLTNRVIDLRRPVMQRNLRLRYQVAMGVRRYLDAQGFID
                     I"
     gene            complement(543..1247)
                     /locus_tag="WIC03_001569"
     CDS             complement(543..1247)
                     /locus_tag="WIC03_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HEZ3884044.1"
                     /translation="MTEPAAEGGKAAKALKKYLITGILVWLPIAVTVWVVSYIVSASD
                     QLVNLLPKQWRPQYVLGFNIPGLGVIIVAIAVLFVTGLFAANVLGRQILAAWDSLLGR
                     IPVVKSIYSSVKKVSESLLSDSSRSFKTPVLVPFPQSGIWTIAFVSGQVSNAVKAALP
                     KDGDYLSVYVPTTPNPTGGYYIMVKKSDVRELDMSVDEALKYVISLGMVIPDDLPVKT
                     LAGPMPSEKADLPEQQ"
     gene            complement(1390..2538)
                     /locus_tag="WIC03_001570"
     CDS             complement(1390..2538)
                     /locus_tag="WIC03_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HEZ3884045.1"
                     /translation="MPTMGAEMNTRNMRYILLTGLLPMASAFGETALQCAALTDNVTR
                     LACYDRIFAAQLPSSAGQEGQESKAILNLTETVRSSLDKGEAVIVVEKGGDALPADSA
                     GETADIYTPLSLMYDLDKNDLRGLLGVREHNPMYLMPLWYNNSPNYAPSSPTRGTTVQ
                     EKFGQQKRAETKLQVSFKSKIAEDLFKTRADLWFGYTQRSDWQIYNQGRKSAPFRNTD
                     YKPEIFLTQPVKADLPFGGRLRMLGAGFVHQSNGQSRPESRSWNRIYAMAGMEWGKLT
                     VIPRVWVRAFDQSGDKNDNPDIADYMGYGDVKLQYRLNDRQNVYSVLRYNPKTGYGAI
                     EAAYTFPIKGKLKGVVRGFHGYGESLIDYNHKQNGIGIGLMFNDLDGI"
     gene            complement(2698..2961)
                     /gene="rpsT"
                     /locus_tag="WIC03_001571"
     CDS             complement(2698..2961)
                     /gene="rpsT"
                     /locus_tag="WIC03_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEZ3884046.1"
                     /translation="MANSAQARKRARQSVKQRAHNASLRTAFRTAVKKVLKAVEAGDK
                     AAAQAVYQESVKVIDRIADKGVFHKNKAARHKSRLSAKVKALA"
     gene            3264..4406
                     /locus_tag="WIC03_001572"
     CDS             3264..4406
                     /locus_tag="WIC03_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ3884047.1"
                     /translation="MKKSVLAVLAALSLAACGGSEKNAVQPQADAASAANAEAAATDT
                     LNIYNWSNYVDESTVEDFKKANNLKLTYDLYENNETLEAKMLTGKSGYDLVVPGIAFL
                     PRQIEAGAYQKVNKDLIPNYKNIDPELLKMLETADPGNQYAVPYFSGVNTIAITAKGK
                     ELLGGKLPENGWDLLFKPEYTHKLKSCGIALWDTPSEMFPILLNYLGKDPKGSNPEDL
                     KAAAEVLKSIRPDVKRFSPSIIDELARGDICLAAGNGGDLNLAKARSEEVKNNVGIEV
                     LNPKGMGFWIESWLIPADAKNVANAHKYINYTLDPEIAAKNGIAVTFAPASKPAREKM
                     PAELVNTRSIFPNEQDMKDGFVMPQMSTDAKKLSVSLWQKIKVGTN"
BASE COUNT         1084 a         1224 c         1140 g         1065 t
ORIGIN      
        1 tcaatgtcga tgaaaccttg cgcgtccaag tagcggcgaa cgcccatagc gacttggtaa
       61 cgcaggcgca ggttgcgctg catcaccgga cggcgcaggt cgataacgcg gttggtcagg
      121 cgaacgtttt cgctgatgtt ttcgtcgtcg atttggaacg gaggcgtagc ggcagcgttc
      181 aacacttcga tttctttggc aaggatttcg attttgccgg aaatcatttt gtcgttggtc
      241 gtgccttcgg gacggttgcg tacgcggccg gtaatgctca aaacgtattc gttgcgggag
      301 gaatcggcag cggcaaacgc ttcgggcgtg tcgggatcga tcacgacttg gacgatgcct
      361 tcgcggtcgc gcaggtcgat aaaaatcaca ccgccatggt cgcgtcgacg gtgtacccag
      421 cctttgacgg taacggtttg gtctaagtat tgctcgctaa tcagaccgca atagttggta
      481 cgcataaaat caccttttat tgatttaaac tgaaaacaga aaatgccgtc tgaacggcgg
      541 ctttattgtt gttcgggcaa atccgccttt tcagacggca taggtcctgc caatgttttg
      601 acgggcaggt cgtcagggat gaccataccc agcgaaatca catatttcaa cgcttcgtcc
      661 acgctcatat cgagttcgcg cacatcgctt ttctttacca taatatagta accgccggtc
      721 gggttgggcg tggtcgggac atacacggaa agataatcgc cgtccttcgg caatgcggcc
      781 ttaaccgcat tcgacacctg accggacacg aatgcgattg tccaaatacc cgattgggga
      841 aacggcacga gtactggtgt tttaaacgaa cggctgctgt cggacagcag cgattcggat
      901 acttttttca cactcgaata gatggacttc acaaccggaa tccgccccaa caggctgtcc
      961 cacgcggcaa gaatctgccg gcccaatacg tttgcggcaa acaatccggt tacaaacaat
     1021 acggcaatgg caacaataat aacgcccagc cccgggatat tgaagcccaa aacatattgc
     1081 ggccgccatt gcttcggcag caggttgaca agctggtcgg acgcggaaac gatataggaa
     1141 accacccaaa ccgttaccgc aatcggcagc cagaccaaaa tgcccgtaat cagatatttt
     1201 tttaacgcct tggcagcttt gccgccttcg gccgcaggtt ccgtcatctt gcttgattcc
     1261 gacaaagtcc gtacaaaccg cacattatac gcgtttgccc ggattcaaac gaaattttta
     1321 tcccgccccg ccaaaccgcc ggcggcttca gacggcacgg caacttgata taccgtctga
     1381 acacgcggtt cagatgccgt ccaagtcgtt gaacatcaac ccgataccga taccgttctg
     1441 cttgtggttg tagtcgatca ggctctcgcc gtaaccgtgg aatccgcgta ccacgccttt
     1501 gagtttgccc ttaatcggaa acgtgtaggc ggcttcaatc gcgccgtagc ccgttttggg
     1561 gttgtagcgc aatacggaat acacattctg cctgtcgttc aggcggtact gcagcttcac
     1621 gtcgccatac cccatatagt cggcaatatc gggattgtcg tttttatcgc cgctctgatc
     1681 gaacgcacgc acccacacgc gcggaatcac cgtcaatttg ccccattcca tgcctgccat
     1741 ggcgtaaatc ctgttccacg aacgcgattc gggacggctc tgtccgttgg actggtggac
     1801 aaaacccgca ccgagcatac gcagcctgcc gccgaacggc aaatccgcct tcacaggctg
     1861 ggtcaggaaa atttcaggtt tgtaatccgt attgcggaac ggcgcggatt tcctgccttg
     1921 gttgtaaatc tgccaatcgg atctttgggt gtagccgaac cacagatccg cgcgggtttt
     1981 aaacaaatct tcggcaattt tgcttttgaa cgaaacctgc aatttggttt ccgcacgttt
     2041 ctgctgtccg aatttttcct gtacagtcgt accgcgcgtc ggcgaactcg gggcatagtt
     2101 gggcgaattg ttgtaccaga gcggcataag gtacatcgga ttgtgttcgc gtacgcccaa
     2161 cagcccgcgc aaatcgtttt tgtccaagtc gtacatcagg ctcaaaggcg tatagatgtc
     2221 ggcggtttcg cccgcactgt cggcaggaag cgcatccccg cctttttcaa caacaatgac
     2281 cgcctcgccc ttatccaggc tgctgcggac ggtttccgtc agattgagta tggctttcga
     2341 ctcctgccct tcctgccctg ccgaagacgg aagctgtgcc gcaaaaatcc tgtcgtaaca
     2401 cgccaaacgc gtaacattgt ccgtcaaagc ggcgcattgc agcgcggtct ctccaaaagc
     2461 ggatgccatc ggcaacagtc ctgtcaaaag aatatagcgc atattccgtg tattcatctc
     2521 cgcccccatt gtcggcatat tggttttcaa acggcatttt atagcggatt cggataaaaa
     2581 ccgcaccctt tccgccattt cgggattttg cccccgcaat acagaaaacc ccgaaaccgt
     2641 cgggcttcag ggttttccgc ttatcgcgta tcaaccgcct tggcggtttt gcaaaaatca
     2701 agccagtgct tttacttttg cagacagacg gcttttgtgg cgtgccgctt tgtttttgtg
     2761 gaacacgccc ttgtcggcga tgcggtcgat gactttgaca gactcttggt aaaccgcttg
     2821 tgcggcagct ttatcgcctg cttcgactgc tttcaatact tttttcactg cagtgcggaa
     2881 tgcggtacgc aggctagcgt tgtgggcgcg ttgtttgacg gactggcggg cacgtttgcg
     2941 tgcttgtgcg ctgtttgcca tattggaatc tcctggaaga aaaataaatt ctgtaaacgc
     3001 gcaattttaa agggggttta cattatatgc aagccttttc tctctatccg cccgtcggac
     3061 accctgacag cgcaaaaaaa cggcaaaatc gtgcggacgt tttccccctc ttttgaaaac
     3121 agggtaattt tttagggaaa tcagtccaat atcactttga gtttgcacaa atttacttta
     3181 caatgcccaa accttgcgcc ggggtgttta tggggcgcgg ggttttcttt ttttccaacc
     3241 tctttcatca ggaatctgcc cgtatgaaaa aatccgtatt agccgttttg gccgcgctgt
     3301 ctctggcagc ctgcggcggc agcgaaaaaa acgccgtgca acctcaagca gacgctgcgt
     3361 ctgctgccaa tgccgaagcc gctgccaccg atactctgaa catctacaac tggtcgaact
     3421 atgttgacga gagtacggtc gaagacttta aaaaagccaa caatctgaag ctgacttacg
     3481 acttgtacga aaacaatgaa acgctggaag ccaaaatgct gaccggcaaa tccggttacg
     3541 atttggttgt gccgggcatc gccttcctgc cgcgccaaat cgaggcgggc gcgtatcaaa
     3601 aagtcaacaa agacctgatt cccaactata aaaacatcga tcccgaactc ttgaaaatgc
     3661 tggaaaccgc cgacccgggc aaccagtatg ccgtccccta tttctccggc gtgaacacga
     3721 ttgcgattac ggcgaagggc aaagagcttt tgggcggcaa gctgcccgaa aacggctggg
     3781 atttgctgtt caaacccgaa tacacccaca agctgaaatc ctgcggcatc gccctgtggg
     3841 acaccccgag cgaaatgttc ccgattttgc tgaactactt gggcaaagac cccaaaggtt
     3901 cgaatcctga agatttgaag gcggcggcgg aagtgttgaa gtctatccgt ccggatgtca
     3961 aacgtttcag cccgtccatc atcgacgagc tggcgcgcgg cgacatctgc ctggcggcag
     4021 gcaacggcgg cgatttgaac ttggcgaaag cacgttccga agaagtgaaa aacaacgtcg
     4081 gcatcgaagt gctgaatccg aaaggcatgg gcttctggat tgagtcttgg ctgattcccg
     4141 ccgatgcgaa aaacgtcgcc aatgcccaca aatacatcaa ctacacgctc gaccccgaaa
     4201 tcgcggcgaa aaacggcatc gccgtaacct tcgcccccgc cagcaaaccg gcgcgcgaaa
     4261 aaatgcctgc cgagctggtg aacacccgtt ccatcttccc gaacgagcag gatatgaaag
     4321 acggttttgt gatgccgcaa atgagcacgg atgcgaaaaa actgtctgtc agcctgtggc
     4381 agaaaatcaa agtcggcacc aactgatttg aagcattaaa aatgccgtcc gaacatcgtt
     4441 cggacggcat tttatattgg attgaaatag aaatatttat atagtggatt aacaaaaatc
     4501 agaacaaggc gac
//
LOCUS       DAWHSK010000162         4470 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.162, whole genome shotgun sequence.
ACCESSION   DAWHSK010000162 DAWHSK010000000
VERSION     DAWHSK010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4470
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.162"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..566)
                     /locus_tag="WIC03_001573"
     CDS             complement(<1..566)
                     /locus_tag="WIC03_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HEZ3884048.1"
                     /translation="MSKNQPAQPTRRTLFKTAIAAGAVGAIGGYLGGKKQGETAERTA
                     ESQHSPQAYPCYGEHQAGIVTPQQAFSIMCAFDVTAQSAKQLENLFRTLTARIEFLTQ
                     GGEYQDGDDKLPPAGSGILGKAFNPDGLTVTVGVGSSLFDGRFGLKDKKPIHLQEMRD
                     FSNDKLQKSWCDGDLSLQICAFTPETCQA"
     gene            complement(830..1996)
                     /locus_tag="WIC03_001574"
     CDS             complement(830..1996)
                     /locus_tag="WIC03_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HEZ3884049.1"
                     /translation="MRKFNLTALSVMLALGLTACQPPEAEKAAPAASGETQSANEGGS
                     VSIAVNDNACEPMELTVPSGQVVFNIKNNSGRKLEWEILKGVMVVDERENIAPGLSDK
                     MTVTLLPGEYEMTCGLLTNPRGKLVVTDSGFKDTANEADLEKLSQPLADYKAYVQGEV
                     KELVAKTKTFTEAVKAGDIEKAKSLFADTRVHYERIEPIAELFSELDPVIDAREDDFK
                     DGAKDAGFTGFHRIEYALWVEKDVSGVKEIAAKLMTDVEALQKEIDALAFPPGKVVGG
                     ASELIEEVAGSKISGEEDRYSHTDLSDFQANVDGSKKIVDLFRPLIEAKNKALLEKTD
                     TNFKQVNEILAKYRTKDGFETYDKLGEADRKALQASINALAEDLAQLRGILGLK"
     gene            complement(1993..2832)
                     /locus_tag="WIC03_001575"
     CDS             complement(1993..2832)
                     /locus_tag="WIC03_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family iron permease"
                     /protein_id="HEZ3884050.1"
                     /translation="MLVAFLIMLREGIEAALIVGIVAGFLKQSGHSKLMPKVWFGVVL
                     ASLMCLGLGYGIHSATGEIPQKQQEFVVGIIGLVAVAMLTYMILWMKKAARSMKRQLQ
                     DSVQAALNRGSGQGWALVGMAFLAVAREGLESVFFLLAVFKQSPTWQMPAGAVAGVLA
                     AAVIGALIYQGGMRLNLAKFFRWTGAFLIAVAAGLLAGSLRALHEAGIWNALQDIVFD
                     SSKYLHEDSPLGVLLGGFFGYTDHPTQGEALVWLLYLIPVITWFLCGSRPSETLTRKE
                     ELK"
     gene            3043..4368
                     /locus_tag="WIC03_001576"
     CDS             3043..4368
                     /locus_tag="WIC03_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HEZ3884051.1"
                     /translation="MKKYLFRAALCGIAAAILAACQSKSIQTFPQPDTSVINGPDRPV
                     GIPDPAGTTVGGGGAVYTVVPHLSLPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCA
                     QAFQTPVHSFQAKQFFERYFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTAQARFPIY
                     GIPDDFISVPLPAGLRSGKALVRIRQTGKNSGTIDNTGGTHTADLSRFPITARTTAIK
                     GRFEGSRFLPYHTRNQINGGALDGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIR
                     IGYADKNEHPYVSIGKYMADKGYLKLGQTSMQGIKSYMRQNPQRLAEVLGQNPSYIFF
                     RELTGSSNDGPVGALGTPLMGEYAGAVDRHYITLGAPLFVATAHPVTRKALNRLIMAQ
                     DTGSAIKGAVRVDYFWGYGDEAGELAGKQKTTGYVWQLLPNGMKPEYRP"
BASE COUNT         1004 a         1316 c         1157 g          993 t
ORIGIN      
        1 gcttggcagg tttcgggggt gaaggcacag atttgcaggc tcaaatcgcc gtcgcaccag
       61 cttttttgca gcttatcgtt ggagaagtcg cgcatttcct gcaaatgaat cggttttttg
      121 tctttgagtc cgaaccgacc gtcaaacagg ctgctgccca cccctacggt aacggtcaac
      181 ccgtcggggt tgaaggcttt gcccaaaatg ccgctgccgg ctggcggaag tttgtcgtcg
      241 ccgtcttggt attcgccgcc ttgggtgaga aactcgatgc gggcggtcag cgtgcggaac
      301 aggttttcca gctgcttggc actttgcgcg gttacgtcga aggcgcacat aatcgaaaac
      361 gcctgctgcg gcgtaacgat gcctgcctga tgttcgccgt agcagggata ggcttggggc
      421 gagtgttggc tttcggcggt gcgttcggcg gtttcgccct gttttttgcc gccgagataa
      481 cctccgattg cgccgactgc tccggctgcg atcgcggttt taaaaagagt gcgcctggtc
      541 ggttgtgcgg gttggttttt gctcatggtg tttccttcaa tatccgtgca gcaggatggg
      601 tatccctgca cgccgttttt ccattaaccg atagcggcag gttgaaatcc tgccctgcaa
      661 tatatggcgg atatagtgga ttaacaaaaa tcaggacaag gcgacgaagc cgcagacagt
      721 ataaatagta cggcaaggca aggcgaggca atgccgtact ggtttttgtt aatccactat
      781 aaaacttcag acggtatctg ccgccaaata ccgtctgaac gcttgcggct tatttcaagc
      841 cgagtatgcc gcgaagttgg gcaaggtctt cggcaagcgc gttaatagag gcctgtaacg
      901 ctttgcggtc ggcttcgccc agcttgtcgt aggtttcaaa accgtcttta gtccggtatt
      961 tcgccagaat ttcgttgacc tgtttgaagt tggtatcggt tttttccaac aaggctttgt
     1021 ttttggcctc gatcagcgga cggaacaaat cgacgatttt tttcgatccg tccacattgg
     1081 cttggaagtc gctcaaatcg gtgtggctgt accggtcttc ttcgccgctg attttactgc
     1141 ccgccacttc ttcaatcagt tcggacgcgc cgccgaccac cttgcccgga ggaaacgcca
     1201 atgcgtcgat ttctttttgc agggcttcga catcggtcat cagtttcgct gcaatttcct
     1261 tcacgccgga cacgtctttt tccacccaaa gggcgtattc gatacggtga aagccggtaa
     1321 atccggcatc tttcgcgccg tctttgaagt cgtcttcacg cgcatcgatg acggggtcga
     1381 gttcgctgaa aagctcggca atcggttcga tgcgttcgta atggacgcgg gtgtcggcaa
     1441 acagggattt cgccttttca atgtcgcctg ctttgacggc ttcggtaaag gttttggttt
     1501 tcgccaccag ctctttaacc tcgccttgaa cgtaggcttt ataatcggcg agcggttggg
     1561 acagtttttc caaatccgct tcgttggcgg tgtctttaaa gccgctgtcg gttaccacca
     1621 gcttgccgcg cggattggtc aaaagaccgc aagtcatttc gtattcgccc ggcaacaggg
     1681 tgacggtcat tttatcggaa agtccggggg cgatgttttc gcgctcgtcc accaccatca
     1741 cgcctttcag gatttcccat tcgagcttgc ggccgctgtt gtttttaata ttgaacacaa
     1801 cctgtccgct cggcacggtc agttccatcg gttcgcaggc attgtcgttg acggcgatac
     1861 tgaccgaacc gccctcgttg gcggattggg tctcaccgga cgcggccggc gcagctttct
     1921 ccgcctccgg cggctggcac gcggtcaaac ctaaggcaag catcacggac aatgcggtca
     1981 aattgaattt tctcatttca gctcctcttt acgggttaaa gtttcagacg gcctgctgcc
     2041 gcacaaaaac caagttatga cgggaataag gtacagcagc caaaccaagg cttcgccctg
     2101 cgtcggatgg tcggtatagc cgaaaaatcc gccgagcagc acgcccaacg ggctgtcttc
     2161 gtgcaaatat tttgatgagt cgaacacaat gtcctgaagc gcgttccaaa tacctgcctc
     2221 atgcagcgcg cgcagcgagc cggcaagcag gccggcggca acggcaatca aaaacgcccc
     2281 cgtccaacga aaaaacttcg ccagattcag gcgcatcccg ccctgataaa tcaacgcgcc
     2341 aatcacggcg gcagccaaaa cccccgctac cgcaccggcc ggcatctgcc acgtcggact
     2401 ctgtttgaat acggcaagca ggaaaaaaac gctctccaga ccttcgcgcg ccacggcaag
     2461 aaacgccata ccgaccaagg cccatccttg gccgctgcca cggttcaaag ccgcctgcac
     2521 agaatcctga agctgccgct tcatcgaacg cgccgccttt ttcatccata aaatcatata
     2581 agtcagcatg gcaacggcaa ccaaaccgat gatgccgacg acgaactcct gctgcttttg
     2641 gggaatctcg cctgttgccg aatggatgcc gtaccccagc cccaaacaca tcaaagaagc
     2701 aaggacaacc ccgaaccaga ccttaggcat cagtttggaa tgtccggact gtttcagaaa
     2761 accggcaacg atgccgacaa tgagcgcggc ttcaataccc tcgcgcaaca taattaaaaa
     2821 agcgaccagc ataaacgcga acgaacaagg atgatgaata atatattatc ggaatatttt
     2881 cattgcttgt aaatacaaat gcaagttatt tttatctgca gtaccgcgcg gcggaaagtt
     2941 ccgcaggctg cagctgcgcc ctgtgttaaa atcccctctc cacggctgcc gcaacgccgc
     3001 ccgaaaccat ctttcttatt actgccggca acattgtcca ttatgaaaaa atacctgttc
     3061 cgcgccgccc tgtgcggcat cgccgccgcc atcctcgccg cctgccaaag caagagcatc
     3121 caaacctttc cgcaacccga cacatccgtc atcaacggcc cggaccggcc ggtcggcatc
     3181 cccgaccccg ccggaacgac ggtcggcggc ggcggggccg tctataccgt tgtaccgcac
     3241 ctgtccctgc cccactgggc ggcgcaggat ttcgccaaaa gcctgcagtc tttcaggctt
     3301 ggctgcgcca atttgaaaaa ccgccaaggc tggcaggatg tgtgcgccca agcctttcaa
     3361 acccccgtcc attcctttca ggcaaaacag ttttttgaac gctatttcac gccgtggcag
     3421 gttgcaggca acggaagcct tgccggtacg gttaccggct attacgaacc ggtactgaag
     3481 ggcgacgaca ggcggacggc acaagcccgc ttcccgattt acggtattcc cgacgatttt
     3541 atctccgtcc ccctgcctgc cggtttgcgg agcggaaaag cccttgtccg catcaggcag
     3601 acgggaaaaa acagcggcac aatcgacaat accggcggca cacataccgc cgacctctcc
     3661 cgattcccca tcaccgcacg cacaacggca atcaagggca ggtttgaagg aagccgcttc
     3721 ctcccttacc acacgcgaaa ccaaatcaac ggcggcgcgc ttgacggcaa agcaccgata
     3781 ctcggttacg ccgaagaccc cgtcgaactc ttttttatgc acatccaagg ctcgggccgt
     3841 ctgaaaaccc cgtccggcaa atacatccgc atcggctatg ccgacaaaaa cgagcatcca
     3901 tatgtttcca tcggaaaata catggcagat aagggctacc tcaaactcgg acaaacctcc
     3961 atgcagggca ttaagtctta tatgcggcaa aatccgcaac gcctcgccga agttttgggg
     4021 caaaacccca gctatatctt tttccgagag cttaccggaa gcagcaatga cggccctgtc
     4081 ggcgcactgg gcacgccgct gatgggcgag tacgccggcg cagtcgaccg gcactacatt
     4141 accttgggcg cgcccttatt tgtcgccacc gcccatccgg ttacccgcaa agcactcaac
     4201 cgcctgatta tggcgcagga taccggcagc gcgattaaag gcgcggtgcg cgtggattat
     4261 ttttggggat acggcgacga agccggcgaa cttgccggca aacagaaaac cacgggatat
     4321 gtctggcagc ttctgcccaa cggtatgaag cccgaatacc gcccgtaaat cggcaaaaca
     4381 atgccgtctg aagcccatcc ctaggcaatc gggcgcatta aggcgaagtt ttgcgatacc
     4441 gcaggcacaa aattcctttc ttatttgacg
//
LOCUS       DAWHSK010000163         4411 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.163, whole genome shotgun sequence.
ACCESSION   DAWHSK010000163 DAWHSK010000000
VERSION     DAWHSK010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4411
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.163"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(10..1101)
                     /gene="mltB"
                     /locus_tag="WIC03_001577"
     CDS             complement(10..1101)
                     /gene="mltB"
                     /locus_tag="WIC03_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HEZ3884052.1"
                     /translation="MKKRKILPLAICLAALSACTAMEARPPRVNEAQAPRADEMKKES
                     RPAFDTAAVPVSDSGFAANANVRRFVDDEVGKGDFSRAEWQDFFDKAAYKADIVKIMH
                     RPSTSRPWYVFRTGNSGKAKFRGARRFYAENRAVVDDVAQKYGVPAELIVAIIGIETN
                     YGKNTGSFRVADALATLGFDYPRRAGFFQKELVELLKLAKEEGGDVFAFKGSYAGAMG
                     MPQFMPSSYRKWAVDYDGDGHRDIWGNVGDVAASVANYMKQHGWRTGGKMLVSATLAP
                     GADVQAIIGEKTALTRTVADLKAYGIIPGEELADDEKAVLFKLETAPGVFEYYLGLNN
                     FYTVWQYNHSRMYVTAVRDIANSLGGPGL"
     gene            complement(1250..2677)
                     /locus_tag="WIC03_001578"
     CDS             complement(1250..2677)
                     /locus_tag="WIC03_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HEZ3884053.1"
                     /translation="MIHTEPSAQPSTMDTAAFLKHIESAFRRIFSDGIDLMRYLPEDK
                     WLALKQAGLLLPFLDKKHGGRKGSQFEIQEVLRIAGHYGVPVTLRTGIEGALVLQPLQ
                     EFGDEAQIAQGLDMVFKGEGGGLGVTEPETSGAAIAREMQSYYEYTDGQTIYVNAAKY
                     WQGNSQSDFLLVAAKERKNGKLAKVIDLLLVPKTYIRCETLASEGLRAVRYAVNRIDA
                     EMPATAVMKLSQSDAAGLRAFQNIFIRSRLQLIGMTHGIMEYILENLERYVRNDIRFV
                     DYERREIRRRHQVSEILYRYVCHSVSPVAPVAHQLMEANIVKTLATEYTYAAAQMLQK
                     LLGAKGFERGHTAGNIAIDIRPFTIFEGPNDMLYAEIYDQFVRATAEEKEAGMKLDKN
                     QTLLDRLQTDARFAAVARDYTLPEDIRSFLQEHTLTDACALQKVFIGKIIARLFVFVQ
                     AEHEDTAAFLLNDIRKDILDCRYCG"
     gene            2862..>4411
                     /locus_tag="WIC03_001579"
     CDS             2862..>4411
                     /locus_tag="WIC03_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEZ3884054.1"
                     /translation="MTYPIPKPREKSRWLNLSQGSLPLALARYLPHRRLKVVLTQDAE
                     QALRLQTAWRFFRPHDTAVFLPDWETLPYERFSPHQDLVSERLSALWQIKSGAADVLF
                     VPVATAMQKLPPVPFLAGRTFWLKTGQTLDIGRLKSDLVDAGYNHVSHVVAAGEFAVR
                     GGIVDLFPMGSEMPYRIDLFDDEIDSIKTFDTETQRTISPVSEIRLLPAHEFPTDNEA
                     QKIFRSRFREEVDGNPNDAAVYKAVSNGHFGAGVEYYLPLFFENELETLFDYIGEDAL
                     FVSLGDVHAEANRFWSDVKSRYAMAQGDETYPPLLPQYLYLSADVFAGRLKNYGQVLP
                     DVSGKEYTLPDLAVNRQADEPLQALKDFQTAFDGRILLCAESLGRRETMLGFLQQNGL
                     KAKPVSDWQGFLSAHEPLMITVAPLAYGFKLGGLQSPSQQQAAPASEGEGDAITDQTE
                     FSASATNPLPSPLPQEREQSAAAVSDDLKTKSSLHPVANNLYGQTRQQAAPSPVGEDW
                     GESKTVAA"
BASE COUNT          925 a         1241 c         1225 g         1020 t
ORIGIN      
        1 taaaaaacat tacaatcccg ggccgccaag cgaattggca atgtccctga ccgccgttac
       61 atacatccgg ctgtggttgt actgccatac cgtataaaaa ttgttcaagc ccaaataata
      121 ttcaaacacg cccggtgcgg tttccagttt gaacaaaacc gccttttcat catctgcaag
      181 ctcttcgccg gggatgatgc cgtacgcctt caaatccgcc accgtccgcg tcagggcggt
      241 tttttcgcca atgattgcct gaacatccgc acccggcgcc aatgttgcag acaccagcat
      301 tttaccgccc gtgcgccaac cgtgctgctt catataattg gcaaccgatg ccgcgacatc
      361 gccgacgttg ccccatatgt cccgatgtcc gtccccgtca taatccaccg cccatttccg
      421 gtagctcgaa ggcataaatt gcggcatacc cattgcaccc gcatagctgc ccttaaaggc
      481 gaaaacatcg ccgccctctt cttttgccag ctttaaaagc tcgaccaatt ctttttggaa
      541 aaacccggcg cggcgggggt aatcgaaacc taaagtcgcc aatgcgtccg ccacgcggaa
      601 actgcccgta tttttgccgt aattcgtttc aatcccgata atcgccacga taagctcggc
      661 gggcacgccg tatttttgcg ccacatcatc gacaactgcg cggttttccg cataaaaccg
      721 gcgcgcgccg cgaaatttcg ccttgcccga atttcccgtg cggaacacat accacggacg
      781 cgatgtggag ggtcggtgca taatcttgac gatgtccgcc ttgtaagccg ctttgtcaaa
      841 aaaatcctgc cattccgccc gggaaaaatc ccctttcccg acttcatcgt ccacaaaacg
      901 gcggacattt gcattggcgg caaacccgct gtcggatacc ggtacggctg ccgtgtcaaa
      961 cgcggggcgg ctttcttttt tcatttcatc cgcgcggggg gcttgggctt catttacccg
     1021 gggcgggcgt gcctccatcg ccgtacaggc agacaaagcc gccaaacaaa ttgccagcgg
     1081 cagtattttt ctctttttca taaatatgtt ccgaacaaat agggtaagtg ggaaagcggc
     1141 gcaaggggcg cggtgcgaaa tgcggcatcc gccgcaatcg gcggctttgt ggaataccgc
     1201 gcgttgcctt ttgcaccgcc tgaaatccgt atgccgccgt atttgcccgc tatccgcaat
     1261 atcggcagtc caatatatct ttgcggatgt cgttcagcag gaaggctgcg gtgtcttcgt
     1321 gttccgcctg tacgaagaca aagagtcggg cgatgatttt gccgatgaag actttttgca
     1381 gggcgcaggc gtcggtcagg gtgtgttcct gcaggaagct gcggatgtct tcgggcaaag
     1441 tgtagtcgcg ggcgacggcg gcaaagcggg catcggtttg caggcggtcg agcagggttt
     1501 ggtttttgtc caacttcatg cctgcttctt tttcttcggc ggtggcgcgg acgaactggt
     1561 cgtaaatttc ggcataaagc atatcgttcg ggccttcaaa aatcgtgaag gggcggatgt
     1621 cgatagcgat attgccggcg gtgtgtccgc gttcaaaacc cttcgcgccc aaaagttttt
     1681 gcaacatttg cgcggcggcg taagtgtatt ccgtggcgag ggttttgacg atgttcgcct
     1741 ccatcagctg atgggcgacg ggggcaacag gtgaaacgga atggcagacg tagcggtaaa
     1801 gaatctcgga aacctgatgg cggcgccgga tttcgcggcg ttcgtaatcg acgaatctga
     1861 tgtcgttgcg gacgtatcgt tccaggtttt caaggatgta ttccataatg ccgtgcgtca
     1921 tgccgatcag ttgcaggcgg ctgcggataa agatgttttg gaacgcgcgc aaaccggcag
     1981 cgtcgctctg ggagagtttc atcacggcgg ttgcaggcat ttcggcatcg atgcggttga
     2041 cggcgtaacg gacggcgcgc aagccttcgg atgcgagggt ttcgcagcgg atgtatgttt
     2101 tggggacgag cagcaggtcg atgactttgg cgagtttgcc gtttttgcgc tctttggcgg
     2161 caacgaggag gaagtcgctt tgcgagttgc cctgccagta tttcgcggcg ttgacgtaaa
     2221 tggtttgtcc gtcggtatat tcgtagtaag actgcatttc tcgggcaatc gccgcgccgg
     2281 aggtttcggg ttcggtaacg cccaaaccac cgccctcgcc tttgaaaacc atgtccaaac
     2341 cctgtgcgat ttgcgcttca tcgccgaact cttgcagtgg ctgcaacacc agcgcgcctt
     2401 cgatgccggt acgcagcgta acgggcacgc cgtaatgccc cgcaatccgc aagacttcct
     2461 gaatttcaaa ctggctgccc ttgcgcccgc cgtgtttttt gtcgaggaag ggcaacagca
     2521 aacccgcctg cttcaaggca agccatttgt cttcgggcag atatcgcatc aggtcgatac
     2581 cgtctgaaaa aatgcggcgg aatgcggatt cgatgtgctt taaaaaagca gccgtgtcca
     2641 tagttgacgg ctgcgcactc ggttcggtgt gtatcatcgg cttcctctgt cggttcccat
     2701 taatcggcgg ccggtcaaac cgcctgccac ggtttagagt taattttcta aactttacca
     2761 caaagtgcgc cgggcaacaa tccgccgacc tttcagacgg catcgcgccc cctcccgtgc
     2821 taaaatgacc gtttgcatca ctgtccgccg attgccgcac tatgacctac cccatcccca
     2881 aaccccgtga aaaatcccgt tggctcaatc tgtctcaagg ctcgctaccc ttggctttgg
     2941 cgcgttatct gccgcatagg cggctcaaag tcgtgctgac ccaagatgcg gaacaggcgc
     3001 tacgccttca gacggcgtgg cggtttttcc gtccgcacga tacggcggtg ttcctgccgg
     3061 actgggaaac gctgccttac gagcgttttt cgccgcatca ggatttggtg tcggagcgtt
     3121 tgtcggcgtt gtggcagatt aaaagcggcg cggcggacgt gttgttcgtg ccggttgcca
     3181 cggcgatgca gaagctgccg cccgtgccgt ttctggcggg gcgcacgttt tggctgaaaa
     3241 cgggacagac tttggatata ggccgtctga aaagcgattt ggtggatgca ggttataacc
     3301 atgtttccca cgttgtcgcg gcaggcgagt ttgccgtgcg cggcggcatt gtggatttgt
     3361 tcccgatggg cagcgaaatg ccgtaccgca tcgatttgtt cgacgatgaa atcgacagca
     3421 tcaaaacctt tgacaccgag acgcagcgca ccatctcccc cgtttccgaa atccgtctgc
     3481 tgccggcgca cgagttcccc accgacaacg aggcgcaaaa aatcttccgc agccgttttc
     3541 gcgaggaagt cgatggcaat ccgaacgatg ccgctgtgta caaagccgtc agcaacggtc
     3601 atttcggcgc gggcgtggaa tactacctgc cgctgttttt tgaaaacgag ttggaaacgc
     3661 tgtttgacta tatcggcgaa gatgcgctgt ttgtctcttt aggcgatgtt catgccgagg
     3721 caaaccgttt ttggagcgat gtcaaatcgc gttacgcgat ggcgcagggc gacgaaacct
     3781 atccgccttt gcttccacag tatttgtatc tctctgccga tgtgttcgca ggccgtctga
     3841 aaaactacgg acaggtgctg cccgatgttt ccggcaagga atacaccctg cccgaccttg
     3901 ccgtcaaccg ccaagcagat gagccgttgc aggcattgaa ggattttcag acggcgtttg
     3961 acggacggat tttgctgtgc gccgaaagtt tgggacggcg cgaaactatg ctcggtttct
     4021 tgcagcaaaa cggtttgaaa gccaaacccg tgtccgactg gcagggcttt ttatcggcac
     4081 acgagccgct gatgattact gttgcgccgt tggcatacgg gttcaaactg ggcgggctgc
     4141 aatctccgag ccaacagcaa gctgctcccg cctccgaagg agaaggcgat gcgattaccg
     4201 accaaaccga attttccgca tccgcaacaa accctctccc tagccctctc ccgcaggaga
     4261 gggaacaaag tgccgccgcc gtttcagacg acctgaaaac caaaagcagc ctgcatcccg
     4321 tcgcaaataa tctgtacgga caaacccgac agcaagctgc cccctccccc gtgggggaag
     4381 attggggaga gagcaaaaca gttgccgctc a
//
LOCUS       DAWHSK010000164         4384 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.164, whole genome shotgun sequence.
ACCESSION   DAWHSK010000164 DAWHSK010000000
VERSION     DAWHSK010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4384
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.164"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(44..3076)
                     /locus_tag="WIC03_001580"
     CDS             complement(44..3076)
                     /locus_tag="WIC03_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEZ3884055.1"
                     /translation="MFWIVLIVILLLALAGLFFVRAQSEREWMREVSAWQEKKGEKQA
                     ELPEIKDGMPDFPELALMLFHAVKTAVYWLFVGVVRFCRNYLAHESEPDRPVPPASAN
                     RADVPTASDGYSDSGNGTEEAETEEAEAAEEEAADTEDIATAVIDNRRIPFDRSIAEG
                     LMPSESEISPVRPVFKEITLEEATRALNSAALRETKKRYIDAFEKNETAVPKVRVSDT
                     PMEGLQIIGLDDPVLQRTYSRMFDADKEAFSESADYGFEPYFEKQHPSAFSAVKAENA
                     RNAPFRRHAGQAEAKSPDVSQGQSVSDGTAVRDACRRVSVNLKEPNKATVSAEARISR
                     LIPESRTVVGKRDVEMPSETENVFTETVSSVGYGGPVYDEAADIYIEEPAAPDAWVVE
                     PPEVPKVPMPAIDIPPPPPVSEIYNRTYEPPTGFEQAQRSRIAETDHLADDVLNGGWQ
                     EETAAIADDGSEGAAERSNGQYLSETEAFGHDSQAVCPFENVPSERPSCRVSDTEADE
                     GAFPSEETGAVSEHLPTTDLLLPPLFNPEATQTEEELLENSITIEEKLAEFKVKVKVV
                     DSYSGPVITRYEIEPDVGVRGNSVLNLEKDLARSLGVASIRVVETILGKTCMGLELPN
                     PKRQMIRLSEIFNSPEFAESKSKLTLALGQDITGQPVVTDLGKAPHLLVAGTTGSGKS
                     VGVNAMILSMLFKAAPEDVRMIMIDPKMLELSIYEGIPHLLAPVVTDMKLAANALNWC
                     VNEMEKRYRLMSFMGVRNLAGFNQKIAEAAARGEKIGNPFSLTPDDPEPLEKLPFIVV
                     VVDEFADLMMTAGKKIEELIARLAQKARAAGIHLILATQRPSVDVITGLIKANIPTRI
                     AFQVSSKIDSRTILDQMGAENLLGQGDMLFLPPGTAYPQRVHGAFASDEEVHRVVEYL
                     KQFGEPDYVDDILSGGGSEELPGIGRSGDDETDPMYDEAVSVVLKTRKASISGVQRAL
                     RIGYNRAARLIDQMEAEGIVSAPEHNGNRTILVPLDNA"
     gene            3241..3921
                     /locus_tag="WIC03_001581"
     CDS             3241..3921
                     /locus_tag="WIC03_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HEZ3884056.1"
                     /translation="MIFQTVWFSDMVLSVSWIVLILILAASAPSAFRSLARYRSALPL
                     CTVIFSAAWCLNASAGGGQLAQMNYHLLAVNLVALMVGTSAAFWLAALLMLPYCLLFA
                     GSAGAYPPNALVLILPALAVNRLSRMLVNRLPPNIFIFIFVNGFLASAAGILLTGLVL
                     TGILDAANAFPSEILWTTALPVFILLAWAEAFLSGISTAIFVALKPHWINTFDDNRYL
                     KSDRGIWR"
     gene            3950..4309
                     /locus_tag="WIC03_001582"
     CDS             3950..4309
                     /locus_tag="WIC03_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEZ3884057.1"
                     /translation="MLSNIIPLSIGAALGATARWLLNLAVPAAMSPATGNLFANWTGA
                     LLIGIFAETINHPQWKLLLITGFLGSLTTLSGFSLETVTLLQSNRPASALANIFLHTA
                     GSLLLTWLGLKIGAAVK"
BASE COUNT          885 a         1286 c         1124 g         1089 t
ORIGIN      
        1 ccgtctgaaa cagtcttcag acggcatttc catttgcaaa aaatcaagca ttgtccaagg
       61 ggacgagaat cgtacggttg ccgttgtgtt ccggtgcgga cacaatgcct tccgcctcca
      121 tctggtcaat cagacgcgcg gcgcggttgt agccgatacg caaggcgcgc tgtacgcccg
      181 aaatgctggc tttgcgcgtt ttcaggacaa cggatacggc ctcgtcgtac atcggatcgg
      241 tttcgtcgtc gccgctgcgc ccgatgccgg gcagctcttc gctgccgccg ccgctcaaaa
      301 tatcgtcaac atagtccggc tcgccaaact gtttcaaata ttcgaccacg cggtgcacct
      361 cttcatccga ggcaaacgcg ccgtgaacgc gctgcggata ggcagtaccc ggcggcagga
      421 acagcatatc gccctgaccg agcaggtttt ccgcgcccat ttggtcgaga atcgtgcggc
      481 tgtcgatttt gctggacact tggaacgcga tacgcgtcgg gatgttcgcc ttaatcagac
      541 ccgtgatgac atcgacgctg gggcgttgtg tggcaaggat aagatggatg cctgccgcgc
      601 gggctttttg ggcgaggcgg gcaatcagtt cctcgatttt cttgcctgcc gtcatcatca
      661 aatcggcaaa ctcatcgacc accaccacga taaacggcag tttttccaaa ggttcgggat
      721 cgtcgggcgt gaggctgaac gggttgccga ttttctcccc ccttgctgcg gcttcggcga
      781 ttttttgatt gaaacccgca agattgcgca cgcccataaa gctcatcagg cggtagcgtt
      841 tttccatttc gttaacacac cagttcagcg cgtttgccgc cagcttcata tcggtaacga
      901 caggggcgag caggtgcggg atgccttcgt aaatgctcaa ttccagcatt ttcggatcga
      961 tcataatcat acgcacgtct tccggcgcgg ctttgaaaag catagacaga atcatcgcgt
     1021 tgacacccac cgatttgccc gaaccggtcg tgccggcaac cagcaaatgc ggtgctttgc
     1081 ccaagtcggt tacgacgggc tgtccggtga tgtcctgacc gagcgcgagc gtcagcttgg
     1141 atttggattc ggcaaactcg ggcgaattga agatttcgct caggcgtatc atttggcgtt
     1201 tcgggttcgg aagttccaaa cccatacagg ttttgccgag gatggtttcg acaacgcgga
     1261 tggaagccac gccgagcgaa cgcgccaagt ctttttccag atttagaacg gaattgccgc
     1321 gcacgccgac atcgggttcg atttcataac gcgtaatcac ggggccggaa taagaatcga
     1381 caaccttgac cttgactttg aactccgcca atttttcttc gatggtgatg ctgttttcca
     1441 acagttcttc ttcggtttgc gtcgcctcgg gattgaacag cggcggcaga agcaggtcgg
     1501 ttgtcggcag gtgttcggat accgcaccgg tttcttcaga tgggaacgcc ccttcatccg
     1561 cttccgtatc cgatacccgg caggacgggc gttcagacgg cacattttca aacggacaaa
     1621 ccgcctgact gtcatgcccg aacgcttcgg tttccgacag atattgcccg tttgaccgct
     1681 ctgccgcacc ttcactgccg tcatctgcaa tagcggcggt ttcctcctgc caacctccat
     1741 tcaaaacatc atcggcaaga tggtcggttt cagcaatgcg gctgcgttgc gcctgctcga
     1801 atcctgtcgg cggctcatag gtacggttgt agatttccga tacgggaggc ggcggcggaa
     1861 tatcgattgc gggcatggga actttcggca cttcgggtgg ttcgaccacc caagcatcgg
     1921 gcgcagcagg ttcttcaata tagatatcgg cagcttcatc ataaaccgga ccgccgtatc
     1981 ccacagacga aacggtttcc gtgaaaacat tttcggtttc agacggcatt tcgacatccc
     2041 gtttcccgac aaccgtccga ctttccggaa tcaggcgcga aatccgcgcc tccgcagaaa
     2101 ccgttgcctt gttcggttct ttcaaattga cggaaacgcg gcggcaggca tcgcggacgg
     2161 ctgtgccgtc tgaaacggac tgtccttggg aaacatccgg ggattttgcc tccgcctgcc
     2221 ctgcatgacg gcggaacggc gcattccgtg cattttcggc tttgactgca gaaaaggcag
     2281 acggatgctg cttctcaaaa tacggctcaa atccgtaatc cgcagactcg gaaaacgctt
     2341 ctttgtccgc atcgaacata cgggaatacg tgcgttgaag cacagggtcg tccaaaccga
     2401 taatctgtag cccttccatc ggggtatcgg acacgcggac tttggggacc gctgtttcgt
     2461 ttttctcaaa tgcatcgata tagcgttttt tcgtttccct taaagccgcg ctgtttaaag
     2521 cacgcgttgc ttcttccaaa gtgatttcct taaaaaccgg acggacgggc gaaatttcgc
     2581 tttcagacgg catcaaccct tcagcaatac tccggtcgaa tgggatgcgg cggttgtcga
     2641 ttacggcagt tgcaatgtct tccgtatcgg cagcctcttc ctccgcagct tctgcttctt
     2701 ccgtttccgc ttcttccgtc ccgtttccac tgtctgaata tccgtcggat gcggtcggaa
     2761 catccgcacg gtttgcagaa gcaggcggaa cgggcctgtc cggttcggat tcgtgcgcca
     2821 gatagtttcg gcagaaacgg acgacaccga caaacagcca atacactgcc gttttgacgg
     2881 catggaaaag catcagggca agttcgggaa aatcgggcat accgtctttg atttcaggca
     2941 gctccgcctg tttttcccct ttcttttcct gccacgcaga aacctcgcgc atccactcgc
     3001 gttcggattg tgcgcggaca aaaaacaagc cggcaagcgc aagcaacaaa ataacgatca
     3061 aaactatcca aaacatatgc cgtttcccgc aagacaaacg attaaacggg tttattttaa
     3121 cgtgtttcca tcccaaaaaa aacaaccggt cggcaattct gccgaaaccg attcggcttt
     3181 cggtttgccc ccgccgacag tataatagcc gtcctttccc ttaatctttg cggcattgcc
     3241 atgattttcc aaacagtctg gttttcagat atggtactgt cggtttcgtg gattgtcctt
     3301 atcctgatac tggcagcttc cgcgccgtcc gcattccgct cgcttgcccg ctaccgttcc
     3361 gcccttcccc tgtgtaccgt aattttttcc gcagcatggt gtctgaacgc ctctgccggc
     3421 ggcggacagc ttgcccagat gaactaccat ctgctcgccg tcaatctggt tgcactgatg
     3481 gtgggcactt ccgccgcctt ttggcttgcc gcgctgctga tgctgcccta ctgcctgctg
     3541 tttgccggtt ctgccggcgc atatccgccg aacgctttgg tgctgattct gcccgcgctt
     3601 gccgtcaacc gcctgtcgcg tatgctggtc aaccgcctgc cgcccaatat tttcatcttt
     3661 attttcgtca acggctttct cgcttccgcc gccggcattc tgttgaccgg gctggtgctg
     3721 accggtattt tggatgctgc aaacgctttt ccgtccgaaa tattgtggac gaccgccctg
     3781 cccgtcttta ttctgctggc gtgggcggaa gccttcctca gcggcatttc aactgccata
     3841 tttgttgcac tgaagcccca ttggatcaac actttcgacg acaaccgcta tctgaagtcc
     3901 gaccgcggca tatggcggta gccttcagac ggcataggca gacaaaacca tgctttccaa
     3961 catcatcccc ctttccatcg gtgcggcact cggtgcaacg gcgcgttggc tgctcaacct
     4021 cgccgttcct gccgccatgt ctcccgccac gggcaacctg tttgccaact ggacgggcgc
     4081 gctgctcatc ggcatctttg ccgaaaccat caatcacccg caatggaagc tgctgctgat
     4141 taccggtttt ctcggcagcc tgaccacgct ttccggattt tcactggaaa ccgtaaccct
     4201 gctccaatcg aatcgtcctg cttcagcact tgccaatatt ttcctacaca cggcaggttc
     4261 gctgctgctg acttggctcg ggctgaagat aggggcggca gtcaaataac cgcaacaaat
     4321 tgcctccctt tgttttgaca taatctataa attagcttcg cttgtttaaa tactggctgt
     4381 gcca
//
LOCUS       DAWHSK010000165         4372 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.165, whole genome shotgun sequence.
ACCESSION   DAWHSK010000165 DAWHSK010000000
VERSION     DAWHSK010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4372
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.165"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(69..2045)
                     /locus_tag="WIC03_001583"
     CDS             complement(69..2045)
                     /locus_tag="WIC03_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEZ3884058.1"
                     /translation="MNEFSLAPIVVVLLVSVITVILCRKFNIPSMLGYLLVGFLAGPG
                     MLSLIPKSHATDYLGEIGIVFLMFSIGLEFSLPKLRAMRRLVFGLGGLQVGVTMLSVM
                     GILMLTGVPFNWAFAVSGALAMSSTAIVSRILSEKTELGQPHGQMAMGVLLMQDIAVV
                     PLMILIPALAGGGDGNIWAALGLAFAKMLLTLGLLFFVGSKIMSRWFRMVAKRKSSEL
                     FMINVLLVTLGVAYLTELEGLSMALGAFVAGMLLSETEYRFQVEDDIRPFRDILLGFF
                     FITVGMKLDIQALIGGWRQVLMLLAMLLVLKALVVFAIAFKMKHSVGDSLKTALYLAQ
                     GGEFGFVMLAIAGQLDMVSPEWEQAATAAVLLSMIIAPFLLGGSDALVGRLVKSSWDM
                     KSLDLHSMLVETMSKSDHVLIVGFGRGGQTVGRVLAQEDIPYFALDLDIARVQVARSA
                     GEPVSFGDAKRREVLEAAGLGRAKMVVLTLNNMHETQHVLDNVLSMYPNMPVYVRATN
                     DDYVKTFTDIGAEEAVSDTKETGLVLAGYAMLGNGASYRHVYQTMANIRHSRYAALEG
                     LFVGSDDEAGFGENGETVRHAFPLAAEAYAVGKTVGTLPMAAYGIKLLFVRRRTGRIE
                     NPDASFTLEGGDVLVVAGKKEEIISFENWSLQGI"
     gene            2194..3033
                     /locus_tag="WIC03_001584"
     CDS             2194..3033
                     /locus_tag="WIC03_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HEZ3884059.1"
                     /translation="MTATAAAIDRLLPQTQCRECGYDGCLPYAQAVATGEAYNLCAPG
                     GEAVIRDISALLGKPLVAPAKTQAKALARIDETACIGCTACIRACPADAIMGAGKLMH
                     TVIADECTGCGLCVAPCPVDCIHMQPVADTVLPRARRFSLSADSRFAAAEHARARYLK
                     RNERKQREADERKAMLAEREAAVRNARPQTPDTPKKPAFNPADLIAKAMAKAQTQQDR
                     LAAADNRKDYQAKQIAEARERAELRRAQRDMKYGSDSEKAAALEYLKQYKAKQEAAQN
                     TAS"
     gene            3234..4265
                     /gene="gap"
                     /locus_tag="WIC03_001585"
     CDS             3234..4265
                     /gene="gap"
                     /locus_tag="WIC03_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEZ3884060.1"
                     /translation="MGIKVAINGYGRIGRQVLRAIYDYQIQDQLQIVAVNASGSLETN
                     AHLTKFDTVHGRFEADVSHDGGNLIVNGDKIPFFSTRNPAELPWKELGVDLVMECTGA
                     FTSKEKAKVHLESGAKKVLISAPGGDDVDATVVYGVNDSILTADMTVVSNASCTTNCL
                     SPVAKVLSESVGIVKGAMTTIHALTNDQTVTDVRHKDLRRARSGVENMIPTKTGAAKA
                     VGLVLPELKDKLDGLAIRVPTVNVSLVDLNFQAARDTTIEEINALMKAASEAGALKGV
                     LGYNTLPLVSMDFNHTTEASHFDATLTKVVDGNMIKVFAWYDNEWGFSCQMLNTARRM
                     FGLEVRPLK"
BASE COUNT         1118 a         1466 c         1002 g          786 t
ORIGIN      
        1 tgaattttgc gaaagcgtta ttaaaccaaa taaccggaaa tggcgcaagc cttatttcgg
       61 catttcattt atattccctg caaactccag ttttcaaaag agataatttc ttcttttttg
      121 cctgcgacca ccaacacgtc accgccttcc aatgtaaacg aggcatccgg gttttcaatc
      181 cggcctgtgc ggcggcggac gaacaagagt ttgatgccgt aagccgccat cggaagcgtg
      241 ccgactgttt tgccgacggc gtatgcttct gcagccaaag gaaaggcgtg gcggacggtt
      301 tcgccatttt cgccgaatcc tgcctcatca tcactaccga caaacaaacc ctccaacgcg
      361 gcataacggc tgtggcggat atttgccatc gtctgataga cgtgccggta cgacgcgccg
      421 ttgcctaaca tcgcatagcc tgccagcacg agtccggttt ctttggtgtc cgacacggct
      481 tcttccgcac ctatatcggt aaacgttttc acataatcgt cgttggtggc gcgcacatat
      541 acgggcatat tgggatacat ggacagcaca ttgtctaaaa cgtgttgcgt ttcgtgcata
      601 ttgttgagcg tcagcaccac cattttcgcc cgccccaaac cggcggcttc caatacttcc
      661 ctgcgtttcg catcgccgaa cgacaccggt tcgcccgcac ttctggcaac ctgcacccgc
      721 gcaatgtcca agtcgagcgc gaaatacgga atatcctctt gggcaaggac gcgtccgacc
      781 gtctgcccgc ccctgccgaa gccgacaatc agcacatggt cggacttgct catggtttct
      841 accagcatac tgtgcagatc gagcgacttc atgtcccagc ttgacttgac caaacgcccg
      901 accagcgcat cgctgccgcc caagaggaag ggcgcgataa tcatcgacag cagcaccgcc
      961 gccgtcgccg cctgttccca ttctggcgaa accatatcaa gctgcccggc aatggccagc
     1021 atcacgaagc cgaactcgcc gccttgtgcg agatacaaag ccgttttgag gctgtcgccg
     1081 accgaatgtt tcattttgaa ggcaatggca aacacaacca gtgccttcaa caccagcagc
     1141 attgccaaca gcatcaatac ctgccgccag ccgccgatca atgcctgaat gtccagcttc
     1201 atgccgaccg tgataaagaa aaagccgagc aaaatatcgc ggaacgggcg gatgtcgtct
     1261 tcgacttgga aacggtattc cgtttccgaa agcagcatcc ccgccacaaa cgcgcccaac
     1321 gccatagaca aaccttccag ctcagtcaga taagccacac ccaaggttac cagcagcaca
     1381 ttgatcataa agagttcgga cgatttgcgt tttgccacca tcctgaacca tcgcgacata
     1441 attttgctgc cgacgaaaaa cagcagcccc agcgtcagca gcatttttgc aaacgccaaa
     1501 cccaaggccg cccaaatatt tccgtcccct ccgcccgcca gcgcgggaat cagaatcatc
     1561 agcggcacga cggcgatgtc ctgcatcagc agcacgccca tcgccatctg accgtgcggc
     1621 tgccccaatt ccgtcttttc cgacaaaatc cggctcacaa tcgccgtgga cgacatcgcc
     1681 aacgcgcccg acacggcaaa cgcccaattg aacggcacgc ccgtcagcat cagtatgccc
     1741 attaccgaca gcatcgtaac gccgacctgc aaaccgccca gcccgaatac cagccgcctc
     1801 atcgccctca acttgggcag cgagaactcc aaaccaatgc tgaacatcag gaacacaatc
     1861 ccgatttcac ccaaataatc cgtcgcatgg cttttcggaa tcaggctgag cataccgggc
     1921 cccgccaaaa agcccaccag caggtagccc agcatggagg gaatgttgaa cttgcggcac
     1981 aggatcaccg taatgaccga caccagcaaa acaaccacaa taggggcaag cgaaaattcg
     2041 ttcatagacc gtccgaacag gaaaatacag aaaaatgccg tctgaaacgg catacgccgc
     2101 cgtattataa caaaacaccg cgcaccatcc gaaacgggcg cggcatacaa ttctgctaaa
     2161 atacgccctt tcgattttga gccgcacacc gacatgaccg ccacagccgc cgccatagac
     2221 cgcctgcttc cccaaaccca atgccgcgaa tgcggctacg acggctgcct gccctacgca
     2281 caggcagtcg caacaggcga agcgtacaac ctctgcgccc cgggcggaga agccgtcatt
     2341 cgggacattt ccgccctgct cggcaaaccc cttgtcgcac ctgccaaaac ccaagccaaa
     2401 gcactcgccc ggatagacga aaccgcctgt atcggctgca ccgcctgcat ccgcgcctgc
     2461 cctgccgatg ccattatggg tgcgggcaaa cttatgcaca ccgtcatcgc cgacgaatgc
     2521 accggctgcg gactctgcgt cgccccctgc cccgtcgact gcatccatat gcagcccgtt
     2581 gccgacaccg tcctgccccg cgcgcgccgc ttcagcctgt ccgccgacag ccgttttgcc
     2641 gccgccgaac acgcgcgtgc gcgctacctc aaacgcaacg aacgcaaaca gcgcgaagcc
     2701 gacgaacgca aggccatgct tgccgaacgc gaagccgccg tccgcaacgc gcgtccgcaa
     2761 acacccgaca caccgaaaaa accggcgttt aaccctgccg acctcatcgc caaagccatg
     2821 gcaaaagcgc aaacccaaca agaccgcctc gccgccgccg acaaccgcaa agactatcag
     2881 gcgaaacaga tagccgaagc ccgcgaacgc gccgagttgc gccgcgccca acgcgatatg
     2941 aaatacggca gcgacagcga aaaagccgcc gccctcgaat atctcaaaca atacaaagcc
     3001 aaacaggaag ccgcacagaa taccgcctcc tgacccttcc ctgatatgcc gtctgaaaac
     3061 cgtttcagac ggcattttat caagctctcc gtccgccgca cccgtgccgt ccgcatctta
     3121 ccgcccaccc ttccggccgc gccgttttca ataaaatatt aattacacgc cactacaaat
     3181 ttgctataat ccgccccgaa aatctaccca acccctcaac aaaggaacaa accatgggca
     3241 tcaaagtcgc catcaacggc tacggacgca tcggccgcca ggttttgcgc gccatctacg
     3301 attatcagat tcaagaccaa ctccaaatcg tcgccgtcaa cgccagcggc agccttgaaa
     3361 ccaacgccca cctcaccaaa ttcgacaccg tgcacggacg ctttgaagcc gacgtatccc
     3421 acgacggcgg caacctcatc gtcaacggcg acaaaatccc cttcttctcg acccgcaacc
     3481 ctgccgaact gccgtggaaa gaactcggtg tcgatttggt catggaatgc accggcgcgt
     3541 tcaccagcaa agaaaaagcc aaagttcatc tggaaagcgg cgcgaaaaaa gtcctcattt
     3601 ccgcaccggg cggcgacgat gtcgatgcaa ccgtcgtgta cggcgtgaac gacagcatcc
     3661 tgaccgccga catgaccgtc gtttccaacg cttcctgcac caccaactgc ctctcgccgg
     3721 ttgccaaagt gttgagcgaa agcgtcggca tcgtcaaagg cgcgatgacc accatccacg
     3781 cgctgaccaa cgaccaaacc gttaccgacg tgcgccacaa agacctgcgc cgcgcccgca
     3841 gcggcgtgga aaacatgatt ccgaccaaaa ctggcgcggc aaaagccgtc ggactggtac
     3901 tgcccgaatt gaaagacaaa ctcgacgggc ttgccatccg cgtgccgacc gtcaacgtat
     3961 cattggtaga tttgaacttc caagccgcgc gcgacacgac aatcgaagaa atcaacgcac
     4021 tgatgaaagc cgcctcggaa gcaggcgcgc tcaaaggcgt tttaggctac aacaccctgc
     4081 ccttggtttc catggacttc aaccacacca ccgaagccag ccacttcgac gcaacactga
     4141 ccaaagtcgt tgacggcaac atgattaaag tcttcgcatg gtatgacaac gaatggggct
     4201 tcagctgcca aatgctgaac accgcacgcc gtatgttcgg acttgaagtg cgcccgctca
     4261 aataagcaat aaaccgtcaa acaaaatgcc gtctgaaacc cgatgttttc aagttccaga
     4321 cggcattttt catttttcac cgtgatttta cccggctgtc gtcatttcta at
//
LOCUS       DAWHSK010000166         4367 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.166, whole genome shotgun sequence.
ACCESSION   DAWHSK010000166 DAWHSK010000000
VERSION     DAWHSK010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4367
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.166"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..719
                     /gene="ftsN"
                     /locus_tag="WIC03_001586"
     CDS             <1..719
                     /gene="ftsN"
                     /locus_tag="WIC03_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HEZ3884061.1"
                     /translation="EPENVGAPLVLINERLEDSNIKGLEASEKLQQAETAKTAPKQAK
                     QRAAEKVPATADSTDTVAVEKPKRTAETKPQKAERTAKAKPKAKETKTAEKVADKPKT
                     AAEKTKPDTAKSDSAVKEAKKADKAESKKTAEKDRSDGKKHETAQKTDKADKTKTAEK
                     EKSGKKAAIQAGYAEKERALSLQRKMKAAGIDSTITEIMTDNGKVYRVKSSNYKNARD
                     AERDLNKLRVHGIAGQVTNE"
     gene            712..1209
                     /locus_tag="WIC03_001587"
     CDS             712..1209
                     /locus_tag="WIC03_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEZ3884062.1"
                     /translation="MNSLPIADLLVSAVIAACIVISTMRGVITEAGSMAAWVVSFFFA
                     KLFAAPFADIAFASFQPRLFALALSFISLFVIACLIQKILRSLLTGAVSAVGLGFANR
                     ILGGVFGALKGILIITLLVMLASKTDLPDTEEWRQSYTLPFFVSLSEAVLNHSGGTAE
                     TPEDD"
     gene            1521..3065
                     /gene="purF"
                     /locus_tag="WIC03_001588"
     CDS             1521..3065
                     /gene="purF"
                     /locus_tag="WIC03_001588"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEZ3884063.1"
                     /translation="MCGVLGLVSHEPVNQFLYDGLQMLQHRGQDAAGIATAEGGTFHM
                     HKGKGMVREVFRTRNMRDLTGNAGIAHVRYPTAGNAGSSAEAQPFYVSSPFGIVLAHN
                     GNLTNTAELYENVCNKHLRHVNTSSDSEVLLNVFAHELRREVSKNADPHRLNADNIFN
                     AVAQVHRLVRGAYGVIAMIAGYGMLAFRDPYGIRPLVLGSQTDSEGRKSYAVASESVA
                     FNALTYDLERDIRPGEAVFVGFDGTMIARQCSDRAKLSPCLFEYVYFARPDSVIDGVS
                     VYQSRLDMGVSLAEKIKRELPVDGIDVVMPIPDTSRPSAMELAVHLDKPYREGLIKNR
                     YIGRTFIMPGQATRKKSVRQKLSPMETEFAGKSVLLVDDSIVRGTTSREIVEMVRAAG
                     ARKVYIASAAPEVRYPNVYGIDMPTREELIANGRSAAEIAAEIGADGIVFQDLGDLEA
                     VVKALNPKIESFDSSCFNGIYQTGDIDDAYLDRLSAEKSGCGGLKIHPSRMEHSISIS
                     DAGDEE"
     gene            3165..3656
                     /gene="greB"
                     /locus_tag="WIC03_001589"
     CDS             3165..3656
                     /gene="greB"
                     /locus_tag="WIC03_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HEZ3884064.1"
                     /translation="MSTETKNYITPAGWQALKDELYQLVNKERPEIVQIVNWAAGNGD
                     RSENGDYLYGKRRMREIDRRIRFLTKRLESAVVVDPELREATDQVFFGATVGLLRGDG
                     REQTVKIVGIDEIDTAQNKISWISPLARCLIKAREGDEVVLNTPEGREEIEILSVEYI
                     KID"
     gene            3715..4341
                     /locus_tag="WIC03_001590"
     CDS             3715..4341
                     /locus_tag="WIC03_001590"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEZ3884065.1"
                     /translation="MRKIRTKICGITTPEDALYAAHAGADALGLVFYPQSPRAVDIIK
                     AQKITAALPPFVSVVALFVNESAQNIRRILAEVPIHIIQFHGDEDDAFCRQFHRPYIK
                     AIRVQTASDIRNAADRFPDAQALLFDAYHPSEYGGTGHRFDWTLLAEYSGKPWVLAGG
                     LTPENVGEAVRITGAEAVDVSGGVEASKGKKDPAKVAAFIATANRLSR"
BASE COUNT         1122 a         1298 c         1090 g          857 t
ORIGIN      
        1 gcgagcctga aaacgtaggc gcgccgctgg tgctgattaa cgagcgcctc gaagacagca
       61 acatcaaagg tttggaagca tccgagaaac tgcaacaggc agaaaccgcc aaaaccgcac
      121 cgaagcaggc aaaacaacgc gctgccgaaa aagtgccggc aactgccgac agtacggata
      181 cggtagcggt tgaaaaaccg aaacgcactg ccgaaacaaa accgcaaaaa gcggaacgca
      241 ctgccaaagc caagcccaaa gccaaagaaa ccaaaaccgc cgaaaaagtt gccgacaaac
      301 cgaaaactgc cgccgaaaaa accaaaccgg atacggcaaa atccgacagc gcggtaaaag
      361 aagcgaaaaa agccgacaag gctgaaagca aaaaaacagc cgaaaaagac cgttcggacg
      421 gcaaaaaaca cgaaacggca caaaaaaccg acaaagcgga caagaccaaa accgccgaga
      481 aggaaaaatc cggtaaaaaa gccgccattc aggcaggtta tgccgaaaaa gaacgcgcct
      541 taagcctcca gcgcaaaatg aaggcggcgg gtatcgattc gaccatcacc gaaattatga
      601 ccgacaacgg caaagtttac cgcgtcaaat caagcaacta taaaaacgca agggatgccg
      661 aacgcgattt gaacaaattg cgcgtacacg gtatcgccgg tcaggtaacg aatgaatagc
      721 ctccccattg ccgacctcct cgtctccgcc gtcatcgccg cctgcatcgt catttccacg
      781 atgcgcggcg tgattacgga ggcaggctca atggcggcat gggtggtttc cttctttttc
      841 gccaaactct ttgccgcacc cttcgccgac atcgcctttg catcgttcca accccgcctg
      901 tttgcattgg ctctgtcgtt catttcccta ttcgtcattg cctgtctgat ccagaaaata
      961 ctccgctcgc tgctgaccgg ggcagtttcg gcggtcggtt tgggctttgc caaccgcatt
     1021 ttgggcggcg tattcggtgc attgaaaggc attttgatta ttaccctgct ggtcatgctc
     1081 gcttcaaaaa ccgacctgcc cgataccgaa gaatggcggc aatcttacac actgccgttt
     1141 ttcgtatcgc tttccgaagc cgtgttgaac catagcggcg gcacggcgga aactccggaa
     1201 gacgattgat tgccatacgg atatttcaga cggcattctc ttttatgccg tctgaaatgt
     1261 tttttccttt cctcagccat atcacagatt atagtggatt aaatttaaac cagtacggcg
     1321 ttgcctcgcc ttgccgtact atttgtactg tctgcggctt cgtcgccttg tcctgatttt
     1381 tgttaatcca ctataaatct tattctccat aaacccacct atctgtcctt cgctcaaata
     1441 ttgtcaacaa ttcaggcaaa aaaccaccgt ttactttata attccgtttt acccttaaac
     1501 ctcgaatcgg agaagacgat atgtgcggcg tattaggttt ggtcagtcat gaacccgtga
     1561 accagtttct gtacgacggc ttgcagatgt tgcagcacag ggggcaggat gcggcgggca
     1621 ttgcaacggc ggaaggcggt accttccata tgcacaaagg caaaggcatg gtgcgcgaag
     1681 tgttccgcac acgcaatatg cgcgatttga ccggcaacgc cggtatcgcc cacgtccgtt
     1741 atcctactgc gggcaacgca ggcagcagcg cggaggcgca gcctttctac gtcagctcgc
     1801 cgttcggcat cgttttggcg cacaacggca acctcaccaa cactgccgaa ctgtatgaaa
     1861 acgtgtgtaa caaacacctg cgccacgtca acaccagctc cgattccgaa gtcttgctca
     1921 acgtattcgc gcacgaatta cgccgcgaag tctctaaaaa cgccgacccg caccggctca
     1981 atgccgacaa tattttcaac gccgttgccc aagtccaccg cctggtgcgc ggcgcatacg
     2041 gtgtgattgc catgattgcg ggctacggta tgctcgcctt ccgcgaccct tacggcatcc
     2101 gcccgctggt attgggttcg caaaccgaca gcgaaggcag aaaatcctat gccgtcgcct
     2161 ccgaatccgt cgccttcaac gcgcttacct acgatttgga acgcgacatc cggccgggcg
     2221 aagcggtatt tgtcggcttt gacggcacaa tgattgcccg ccaatgcagc gaccgtgcca
     2281 aactcagccc ctgccttttt gaatatgttt attttgcccg ccccgactct gtgattgacg
     2341 gcgtatccgt ttaccaatcg cgcttggata tgggcgtgtc gctggcggaa aaaatcaaac
     2401 gcgagctgcc cgtggacggc atcgatgtcg tgatgcccat tcccgacacc agccgcccca
     2461 gcgcgatgga gcttgccgtc catctcgaca aaccctaccg cgagggtttg attaaaaacc
     2521 gctatatcgg ccgcaccttt attatgcccg gacaggcgac gcgcaaaaaa tccgtgcgcc
     2581 aaaaactcag cccgatggaa accgaatttg caggcaaaag cgtgttgctg gtggacgact
     2641 ccatcgtgcg cgggacgacc agccgcgaaa tcgtcgaaat ggtacgcgcg gcgggcgcgc
     2701 gcaaagtcta tatcgcctcc gccgcgcccg aagtgcgcta tcccaatgtg tacggcattg
     2761 atatgcccac gcgcgaagag ttgattgcca acgggcgcag cgcggcggaa atcgccgccg
     2821 aaatcggcgc ggacggcatc gtatttcaag acttgggcga tttggaagcc gtcgtcaaag
     2881 cactcaatcc gaaaatcgaa tccttcgatt cgtcctgttt caacggcatt tatcagaccg
     2941 gcgacatcga tgatgcctac ctcgaccgcc tgtctgccga gaaatccggc tgcggcggtc
     3001 tgaaaatcca cccgagcagg atggaacaca gcatcagcat cagcgatgcg ggcgacgaag
     3061 aataaggctt cccaaaccgc gatgccgtct gaaatccgaa cgccgggttt tcagacggca
     3121 tttcccatat cggcatcaca tttcccgttt catccgcaca caatatgagt accgaaacca
     3181 aaaactacat tacgcccgcc ggatggcagg cattgaaaga cgagctttat caactggtca
     3241 acaaagaacg ccccgaaatc gtccaaatcg tcaactgggc ggcaggcaac ggcgaccgca
     3301 gcgaaaacgg cgactatctt tacggcaaac gccgtatgcg cgaaatcgac cgccgcatcc
     3361 gtttcctgac caagcgtttg gagtccgccg tcgtcgtcga tcccgaattg cgcgaagcga
     3421 ccgaccaagt gtttttcggc gcgaccgtcg gtctgttgcg tggcgacggg cgcgagcaaa
     3481 ccgtcaaaat cgtcggcatc gatgaaatcg ataccgcgca aaacaaaatt tcttggattt
     3541 cgccgctggc tcgttgtctg attaaagcgc gtgaaggcga cgaagtggtt ttaaacacgc
     3601 cggaagggcg tgaggaaatt gaaatccttt ctgtcgaata catcaaaatc gactgatcgg
     3661 gaatgccgtc tgaaacggca caaacgcgcc gtttcccaaa taaaggaata ccgaatgagg
     3721 aaaatccgca ccaaaatctg cggcatcacc acaccggaag acgcactgta tgccgcccac
     3781 gccggcgcag acgcattggg actggttttt tacccccaaa gcccccgcgc tgtcgacatc
     3841 attaaagcac aaaaaatcac cgccgcactg ccgccgtttg tcagcgttgt cgcccttttc
     3901 gtcaacgaaa gcgcgcaaaa catccgccgc atccttgccg aagtaccgat acacatcatc
     3961 caattccacg gcgacgaaga cgacgcattc tgccgccagt tccaccgccc ctatatcaag
     4021 gccattcgtg ttcagacggc atcagacatc cgaaacgccg ccgaccgctt ccccgacgct
     4081 caggcactgc ttttcgatgc ctaccatcct tcggaatacg gcggcaccgg acaccgcttc
     4141 gactggacgc tgctggcgga atattcgggc aaaccgtggg tacttgccgg cgggctgacc
     4201 cctgaaaacg tcggcgaagc cgtccgcatc accggagcgg aagcggtcga tgtatccggc
     4261 ggcgtggaag cgtctaaagg caaaaaagac ccagccaaag tcgccgcctt tatcgcaacc
     4321 gccaaccgcc tatcccgtta aagcaacaaa aattgccgcc ggaatga
//
LOCUS       DAWHSK010000167         4342 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.167, whole genome shotgun sequence.
ACCESSION   DAWHSK010000167 DAWHSK010000000
VERSION     DAWHSK010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4342
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.167"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..507
                     /locus_tag="WIC03_001591"
     CDS             <1..507
                     /locus_tag="WIC03_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HEZ3884066.1"
                     /translation="AGMYCLMLLVFLIGVQLKSSGLSLRQVLVNRRGIRLSVWFMLSS
                     LSGGLLFAASADGVSWTKGLAMASGFGWYSLSGLVMTEAYGAVWGSIMLLNDLARELF
                     ALAFIPLLMKRFPDAAVGVGGATSMDFTLPVIQGAGGLEVVPVAVSFGVVVNIAAPFL
                     MVVFSTLG"
     gene            550..1323
                     /locus_tag="WIC03_001592"
     CDS             550..1323
                     /locus_tag="WIC03_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEZ3884067.1"
                     /translation="MKPKIQRHGEILSLVRRHQFMSVDELAAALDVTPQTIRRDIREL
                     EEGGSLKRHHGGASSGGNLPEGLPASRQTRCQNEKNAIARLIAEHIPDGSSLFVSIGT
                     TMEAVASELVRRRGSLRIITNNIHVASIVSARTDYTVIITSGVVRPLDGGITGVATVD
                     FINQFKVDYAVMSTHGVESDGSLLDDDYKEVSVMQAMIANARVRFLGVDHSKFRSNAL
                     VRLGDITAFDKVFTDRLPDTAMQKMLKEAGVECLIADAV"
     gene            1435..3123
                     /locus_tag="WIC03_001593"
     CDS             1435..3123
                     /locus_tag="WIC03_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HEZ3884068.1"
                     /translation="MLNKDQFADNHFIRTFIEEDLKSGKHETIQTRFPPEPNGYLHIG
                     HAKSICLNFGLAYIYDGLCNLRFDDTNPEKENDEYVNAIKEDVEWLGFHWAGEPRFAS
                     NYFDQLYDYAVGLVKDGKAYVDDLTPEEMREYRGTLTEAGKNSPYRDRSIEENLDLFT
                     RMKNGEFPDGSKTLRLKIDMASGNINMRDPVIYRIRRAHHHNTGDKWCIYPMYDYTHC
                     ISDAIEGITHSLCTLEFEAHRPLYDWVLDNIPAPHATRPRQYEFSRLELLYTITSKRK
                     LNQLVVEKHVSGWDDPRMPTISGMRRRGYTPEGLRLFAKRAGISKSENIVDMSVLEGA
                     IREELENSAPRLMAVLNPLKVTLTNFETGRTQSRRAAFHPNHEEMGDREVPVSQTIYI
                     EADDFAENPPKGFKRLIPGGEVRLRHGYVIKCDEVVKDEAGNVVELKCSIDHDTLGKN
                     PEGRKVKGVIHWVSAEHAAEIKVRLYDRLFTVERPDAVRGEDGEYLPFTDFLNPESVK
                     EITAYAEPAAKDLPAESRWQFERIGYFVTDRKDHGKDTPVFNRTVTLKDSWQPK"
     gene            3216..4172
                     /gene="gshB"
                     /locus_tag="WIC03_001594"
     CDS             3216..4172
                     /gene="gshB"
                     /locus_tag="WIC03_001594"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HEZ3884069.1"
                     /translation="MKVLFIADPMASFKTYKDTTYAMMREMAKRGWRLFHTLSGELSV
                     KNGLVVADASAFEFLGAQDDHDKNWFAAADKVQTALKAFDAVIMRTDPPFDMQYLYAT
                     QLLTLAEQQGAKVFNSGQSMRDFNEKLAILNFSRFTAPTLVTTRSADVRAFLKEHGDI
                     IIKPLDGMGGMGIFRLTEKDPNIGSILETLMRLDSRTIMAQRYIPEIVHGDKRILIIG
                     GEVVPYALARIPQNGETRGNLAAGGRGVAQELDRRDREIAETLAPELKRRGILLAGLD
                     VIGSNLTEVNVTSPTGFQEIMKQKGFDVAAMFADAVAAWSVR"
BASE COUNT         1031 a         1217 c         1181 g          913 t
ORIGIN      
        1 gcgggcatgt attgtctgat gctgctggtg ttcctcatcg gcgtacagct caaaagcagc
       61 ggcctatcgt tgcggcaggt tttggtcaac cgcaggggta ttcggttgtc ggtctggttt
      121 atgctttcat ctctttcggg cgggctgctg tttgccgcat cggcagacgg tgtgtcgtgg
      181 acgaaaggtt tggcgatggc ttccggcttc ggttggtatt ccctctcggg tttggtcatg
      241 accgaggctt acggcgcggt atggggcagc atcatgctgc tgaacgattt ggcacgagag
      301 ctgtttgcac tggcatttat cccgctgctg atgaagcgtt ttccggatgc ggcggtgggg
      361 gttggcggtg cgaccagtat ggattttaca ttgcccgtga ttcagggtgc gggcggtttg
      421 gaagtcgtgc cggtagcggt cagcttcggc gtggtggtca atattgccgc cccgtttctg
      481 atggtggtgt tttccacgct gggctgaacg cggtaaaatc ggcatcccga tgcaaggagg
      541 cagaaaacga tgaaaccgaa aatccaaagg cacggagaga ttttaagcct tgtccgccgg
      601 catcagttta tgtcggtgga cgagcttgcc gccgcattgg acgttacccc gcagacgata
      661 cgccgcgaca tccgcgagtt ggaggagggc ggcagcctga aacgccatca cggcggcgca
      721 tcttcgggcg gaaacttgcc ggaggggctg cctgccagcc gccaaacccg gtgtcaaaac
      781 gaaaaaaacg ccattgcccg gctgattgcg gaacacatcc ccgacggttc gtcgctgttt
      841 gtcagtatcg gcacgacgat ggaagccgtg gcatcggagc tggtcaggcg gcgcggcagc
      901 ctgcggatta ttaccaacaa tatccacgtc gcctccatcg tttcggcgcg gacggattac
      961 acggtcatta tcacttccgg agtcgtccgc cctttggacg gcggtattac cggcgtggca
     1021 accgtcgatt ttatcaacca gttcaaagtc gattatgccg tgatgagtac gcacggcgtg
     1081 gaaagcgacg gttcgctttt ggacgacgac tacaaagaag tcagcgtgat gcaggcgatg
     1141 attgccaatg cgcgcgtccg cttcctcggc gtggaccaca gtaaattccg cagcaacgcg
     1201 ctggtcaggc tcggcgacat tacggcgttt gacaaagtat ttaccgaccg gctgcccgat
     1261 accgcgatgc agaagatgct gaaagaggcg ggggtggaat gcctgattgc cgatgccgtc
     1321 tgaacgctat gtcaaagcgc gcaagtcggg tacaataaac acatcatcaa accgcttcag
     1381 acggcataag gaaccgcccc aatgccgtct gaagccatct gttcaaagaa aactatgctc
     1441 aataaagacc aatttgcgga caaccatttt atccgcacct tcatcgaaga agacctcaaa
     1501 agcggcaaac acgaaaccat ccaaacccgt tttccgccgg agcccaacgg ctacctgcac
     1561 atcggacacg ccaaatccat ctgcctgaac ttcggtttgg cgtatattta cgacggcttg
     1621 tgcaacctgc gttttgacga taccaacccc gaaaaagaaa acgacgaata cgtcaacgcc
     1681 atcaaagaag acgttgagtg gctcggtttc cattgggcgg gcgagccgcg tttcgcttcc
     1741 aactatttcg accagcttta tgactacgcc gtcggtttgg ttaaagacgg caaagcgtat
     1801 gtcgatgatt tgacgccaga agaaatgcgc gaataccgcg gtacgctgac cgaagcaggc
     1861 aaaaacagcc cttatcgcga ccgcagtatc gaagaaaacc tcgacctgtt cacgcgcatg
     1921 aaaaacggtg agttccccga cggcagcaaa accctgcgcc tgaaaatcga catggcatcc
     1981 ggcaacatca atatgcgcga tccggtcatc taccgcatcc gccgcgccca tcaccacaac
     2041 accggcgaca aatggtgcat ctatccgatg tacgactaca cgcattgtat ttccgatgcc
     2101 atcgaaggca tcacgcattc cttgtgtacg ctcgaatttg aagcccatcg tccgctgtat
     2161 gactgggtgt tggacaacat ccccgcgccg cacgccacac gtccgcgcca atacgagttt
     2221 tcacgcttgg agcttttgta caccattacc tccaaacgga aattgaatca gttggttgtg
     2281 gaaaaacacg tttccggctg ggacgatccg cgtatgccga ccatttccgg tatgcgccga
     2341 cgcggctaca cgcccgaagg gctgcgcctg tttgccaaac gcgccggtat ttccaaatct
     2401 gaaaacatcg tcgatatgag cgtgttggaa ggcgcgattc gcgaagagtt ggaaaattcc
     2461 gccccacgcc tgatggcggt gttgaacccg ctcaaagtaa ccctgaccaa ctttgaaacc
     2521 ggcagaaccc aaagccgccg tgccgcgttc catccgaacc acgaagaaat gggcgatcgc
     2581 gaagtacctg tttcacaaac catttacatc gaagccgacg actttgccga aaatccgccc
     2641 aaaggcttca aacgcctgat tcccggcggc gaagtacgct tgcgccacgg ctatgtcatc
     2701 aagtgcgatg aagtcgtcaa agacgaagca ggcaatgtgg ttgaactcaa atgcagcatc
     2761 gaccacgaca ccttgggcaa aaatccagaa ggccgtaaag ttaaaggcgt gattcattgg
     2821 gtttccgccg aacacgccgc cgaaatcaaa gtccgtctgt acgaccgcct ctttaccgtc
     2881 gagcgtcccg atgccgtgcg cggcgaagac ggcgaatacc tgccgttcac cgatttcctc
     2941 aatccggaat ccgtcaagga aatcaccgcc tacgccgaac ctgccgccaa agatttgccg
     3001 gcagaaagcc gttggcagtt cgagcgtatc ggctacttcg taaccgaccg taaagaccac
     3061 ggcaaagaca cgccggtgtt taaccgcacg gtcacgctga aagattcttg gcagcctaag
     3121 taaaacccca tccttgccgt ctgaatattg ttcggacggc atttcccctt tgcccgcgaa
     3181 atgcggcaca ttcggcacac cggaaaggaa atatgatgaa agtcctcttt atcgccgacc
     3241 cgatggcaag tttcaaaacc tacaaagaca ccacctacgc catgatgcgc gagatggcaa
     3301 aacgcggttg gcggctgttt cataccttga gcggggaatt gtcggttaaa aacggtctgg
     3361 tggttgctga tgcgtctgct tttgagtttc tcggcgcaca agacgaccac gacaaaaact
     3421 ggtttgccgc cgccgacaaa gttcagacgg cattgaaagc atttgatgcc gtgattatgc
     3481 gtaccgatcc gccgttcgat atgcaatacc tctacgccac ccaattactg acgctggcgg
     3541 aacagcaggg cgcgaaagtc tttaacagcg gacagtcgat gcgtgacttc aatgaaaaac
     3601 tggcgatttt gaacttcagc cgctttaccg cgcccacgct ggtaacgacc cgttccgccg
     3661 atgtccgcgc atttttgaaa gaacacggcg acattatcat caaaccgctg gacggtatgg
     3721 gcggtatggg tattttccgc ctgaccgaaa aagaccccaa catcggcagc atcctcgaaa
     3781 ccctgatgcg gcttgattcc cgcaccatta tggcgcaacg ctacattccc gaaatcgtac
     3841 acggtgacaa acgcatcctg attatcggcg gcgaagtcgt cccctatgct ttggcgcgta
     3901 tcccgcaaaa cggcgaaaca cgcggcaacc ttgcggcagg cgggcgcggc gtggcgcagg
     3961 aattggacag acgcgaccgc gaaattgccg aaacgctcgc ccccgaactc aaacggcgcg
     4021 gcatcctgct ggccggtttg gacgttatcg gcagcaacct gaccgaagtc aacgtaacca
     4081 gcccgaccgg attccaagaa attatgaaac aaaaaggttt cgacgtggcg gcaatgtttg
     4141 ccgatgccgt tgccgcgtgg tcggtacgtt aaaccgatgc cgtttgaaag gcttttgctt
     4201 cacaaccgtt tggtttgttc gggcaggcag ggttttctct cagccggcag ggatgcggtt
     4261 aatgccgtac ggacaccccg ccgccccgtt ttcagacggt atatatagtg gattaacaaa
     4321 aatcaggaca aggcggcgaa gc
//
LOCUS       DAWHSK010000168         4309 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.168, whole genome shotgun sequence.
ACCESSION   DAWHSK010000168 DAWHSK010000000
VERSION     DAWHSK010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4309
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.168"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..247)
                     /locus_tag="WIC03_001595"
     CDS             complement(<1..247)
                     /locus_tag="WIC03_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884070.1"
                     /translation="MIEMSKDYRNDLYDVYVSYPPQVDRGLIRECLRENLGEEKAEGL
                     IESLDSKPQVLVEEKCTWAKREELHDYFSYLGLDIITR"
     gene            complement(244..390)
                     /locus_tag="WIC03_001596"
     CDS             complement(244..390)
                     /locus_tag="WIC03_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884071.1"
                     /translation="MPSERSDGIFTFGKYIIGMNSHIIRLFEDESGFPKGTGGTVSGV
                     FVPL"
     gene            complement(407..745)
                     /gene="erpA"
                     /locus_tag="WIC03_001597"
     CDS             complement(407..745)
                     /gene="erpA"
                     /locus_tag="WIC03_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEZ3884072.1"
                     /translation="MSDESPIIFTDSCCAKVADLIAEENNPDLKLRVFVNGGGCSGFQ
                     YGFTFDEIKNDDDFEIEKNGLVFLVDPMSYQYLVGAEIDYTESLQGSQFVIRNPNAET
                     TCGCGSSFSV"
     gene            complement(877..1563)
                     /locus_tag="WIC03_001598"
     CDS             complement(877..1563)
                     /locus_tag="WIC03_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ3884073.1"
                     /translation="METFKDRLVFLWKSEAKQAKIASDIEMTIAGFSRIWNEGGLPKS
                     ETLKKIKQLKGCSIDWLLTGEGNPFPDEAPKKSLAYDTLGNEVDTDEFVFVPRYDIRA
                     AAGYGQFVDHEEPVFTMAFRRHWIENYITRDTKNLSVISVKGDSMEGVLNDGDSILVN
                     HGENTPRDGLYVLRINENLLVKRLQIVPGGIINVISANEAYPAFEINLNDLTDDVEII
                     GRVEWFGRTI"
     gene            1749..2021
                     /locus_tag="WIC03_001599"
     CDS             1749..2021
                     /locus_tag="WIC03_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884074.1"
                     /translation="MKKTGKYLIYAAAFTAFCFAFQENRSEAKQPDITLSASLCEQFN
                     ILNAKDMDAEQVSLSKECDIIESSHDWEKEYGNLNEQEMLAGIVYE"
     gene            2229..4157
                     /gene="dnaK"
                     /locus_tag="WIC03_001600"
     CDS             2229..4157
                     /gene="dnaK"
                     /locus_tag="WIC03_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEZ3884075.1"
                     /translation="MAKVIGIDLGTTNSCLAISENGQTKVIENAEGARTTPSVIAYLD
                     GGEILVGAPAKRQAVTNAKNTIYAAKRLIGHKFEDKEVQRDIESMPFEIIKANNGDAW
                     VKAQGKELSPPQISAEVLRKMKEAAEAYLGEKVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLEVKRIINEPTAAALAFGMDKGDNKDRKVAVYDLGGGTFDISIIEIANLDGDKQFE
                     VLATNGDTFLGGEDFDQRLIDYIIDEFKKEQGIDLKQDVMALQRLKEAAEKAKIELSS
                     GQQTEINLPYITMDATGPKHLAMKITRAKFESLVEDLIARSIEPCKIALKDAGLSTGD
                     IDDVILVGGQSRMPKVQEAVKDFFGKEPRKDVNPDEAVAVGAAIQGEVLSGGRSDVLL
                     LDVTPLSLGIETMGGVMTKLIQKNTTIPTKASQVFSTAEDNQSAVTIHVLQGERERAS
                     ANKSLGQFNLGDIAPAPRGMPQIEVTFDIDANGILHVSAKDKGTGKAANITIQGSSGL
                     SEEEIERMVKDAEANAEEDKKLTELVASRNQAEALIHSVKKSLADYGDKLDAAEKEKI
                     EAALKEAEEAVKGDDKTAIDAKAEALGTASQKLGEMVYAQAQAEAQAGESEQANASAK
                     KDDDVVDADFEEVKDDKK"
BASE COUNT         1322 a         1127 c          865 g          995 t
ORIGIN      
        1 tccgggtaat aatatccaaa cccaaatagc tgaaataatc gtgcaactct tcccgtttcg
       61 cccaagtgca tttttcctca accagcactt gaggtttgga atcgagcgat tcgatcaatc
      121 cttccgcctt ttcctcgccg agattctccc tgaggcactc ccggataagc ccgcgatcca
      181 cttggggcgg gtaagaaaca tatacatcgt acaaatcgtt tcggtaatct ttactcatct
      241 caatcataag ggaacaaata cgcctgatac cgttccgccc gttcctttcg ggaaacccga
      301 ttcatcttca aacaatcgga ttatatgtga attcatcccg ataatatatt ttccaaaagt
      361 aaaaatgccg tctgaacgtt cagacggcat cacacaaacc aagcggtcat acggaaaacg
      421 acgatccgca accgcaggtt gtttccgcat tcggattgcg gatgacgaat tgcgaaccct
      481 gcaaactttc cgtatagtcg atttccgcac cgaccagata ttgatagctc atcggatcga
      541 ccaaaaagac caaaccgttt ttctcaattt caaaatcgtc gtcgtttttg atttcgtcaa
      601 aagtaaatcc gtactggaaa cccgaacagc cgccgccatt gacaaaaacc cgcaatttca
      661 aatcgggatt gttttcttcg gcaatcaaat cggcaacttt ggcacagcag ctgtcagtaa
      721 aaataatagg gctttcatcc gacatggtgg tattccttaa tataatattt tctgcattct
      781 ctctcaaacc cccttcaaag gcaagcaggc ttcgcctttg aaaaaggaat tgttccaata
      841 gataagtttg accgcctgca atttcaagcc ccaaactcaa atcgtcctgc cgaaccactc
      901 gacacgcccg ataatctcca catcatcggt caaatcgttc aaattgattt caaaagcagg
      961 ataagcctcg tttgcagaaa ttacattgat aatcccgccc ggcacaatct gtaaacgttt
     1021 aaccagcaga ttttcattaa tccgcaacac atacagaccg tccctcggcg tattttcccc
     1081 atgattgacc aaaatcgaat cgccgtcatt caaaaccccc tccatcgaat cccccttgac
     1141 ggaaattaca gacaggtttt tcgtatcgcg ggtaatataa ttctcaatcc aatgccgtct
     1201 gaacgccatt gtaaataccg gttcctcatg atcgacaaac tgcccgtatc ccgcagccgc
     1261 ccgaatatca tatctcggca caaagacaaa ctcgtccgta tcaacttcat tgcccaaggt
     1321 atcgtaagca agggattttt ttggggcttc atccggaaac ggattaccct ccccggtcag
     1381 cagccaatcg atactacacc ccttcaactg cttgattttt ttcaatgttt cagacttcgg
     1441 cagaccgcct tcattccata tcctgctgaa gcccgcaatc gtcatttcaa tatcggatgc
     1501 gatttttgcc tgcttcgctt cgcttttcca taaaaaaacc agtctgtctt taaaggtttc
     1561 cataaatatc cttccgctcc ccttcattca atcatctaaa gtaaacatga aatatatttt
     1621 accttagcta tcccaaaaac ggaacaacta tgataaacta aatttaaatt atgatttttc
     1681 ttactaaata cataattttt aagattttcc ataggtttaa tcctgaattg acagaaaaag
     1741 gatagaaaat gaagaaaacc ggcaaatatc ttatctatgc tgcggcattt accgcattct
     1801 gctttgcctt ccaagaaaac cgttctgaag ccaaacagcc tgacatcact ttatccgcat
     1861 ccctgtgcga acaattcaac atactgaacg ccaaagatat ggatgcagaa caagtctccc
     1921 tttccaaaga atgcgacatc atcgagtctt cacacgactg ggaaaaagag tacggcaact
     1981 tgaacgaaca ggaaatgctc gccggcatcg tctatgaata aacctgcctg ccatttgaaa
     2041 cattatgctt gaatgcattg gagccaaatg tattaaatca aatataaaac caatatattc
     2101 ataaagttat atacttatag ccatgctgta gcttgaaaca gcccgccgcc cgccctattt
     2161 acagcccatc gggacaaaat gttccaacat atttctcaaa ataagcaaaa tcaaatagga
     2221 ttatccacat ggcaaaagta atcggtatcg acttaggtac aaccaactct tgtttggcca
     2281 tttccgaaaa cggtcaaacc aaagttatcg aaaacgcaga aggcgcacgc accacgccgt
     2341 ccgttatcgc ttatttggac ggcggcgaaa tcctcgtcgg cgcgcctgcc aaacgccaag
     2401 ccgtaaccaa cgctaaaaac actatttacg ccgccaaacg tttgatcggc cacaaatttg
     2461 aagacaaaga agtccaacgc gacatcgaat ctatgccttt tgaaatcatc aaagccaaca
     2521 acggcgacgc atgggtgaaa gcgcaaggca aagagctgtc tcctcctcaa atttccgcag
     2581 aagtcctgcg taaaatgaaa gaagccgctg aagcttactt gggcgagaaa gtcaccgaag
     2641 ccgtgattac cgttcctgcc tacttcaacg acagccaacg tcaagcaact aaagacgcag
     2701 gccgcatcgc aggtttggaa gtaaaacgta tcatcaacga accaaccgca gccgctttgg
     2761 cattcgggat ggacaaaggc gacaacaaag accgcaaagt agccgtatat gacttgggtg
     2821 gcggtacctt cgatatctcc atcatcgaaa ttgccaacct cgacggcgac aaacaattcg
     2881 aagtattggc aaccaacggc gatactttct tgggcggtga agacttcgac caacgcttga
     2941 tcgactacat cattgacgag ttcaaaaaag aacaaggcat tgatttgaaa caagacgtaa
     3001 tggctctgca acgtctgaaa gaagctgctg agaaagccaa aatcgaattg tccagcggcc
     3061 agcaaaccga aatcaacctg ccgtacatca ctatggacgc aaccggtccg aaacacttgg
     3121 caatgaaaat tacccgtgcc aaattcgaaa gcctggttga agacttgatt gcccgctcta
     3181 tcgaaccttg caaaattgca ttgaaagatg ccggcttgag caccggcgac atcgacgacg
     3241 tgattttggt cggcggtcag tcccgtatgc cgaaagtaca agaagccgtt aaagacttct
     3301 tcggcaaaga gcctcgtaaa gacgtgaacc ctgatgaagc cgttgccgta ggcgcagcga
     3361 ttcaaggcga agtattgagc ggtggccgca gcgacgtatt gctgctggac gtaacccctc
     3421 tgtccttggg tatcgaaacc atgggcggcg tgatgaccaa gctgattcaa aagaacacga
     3481 ccattccgac taaagcgtct caagtgttct ctactgccga agacaaccaa agcgcagtaa
     3541 ccatccatgt actgcaaggc gaacgcgaac gcgcttctgc caacaaatct ttgggtcagt
     3601 tcaacttggg cgacatcgca cccgcaccgc gcggtatgcc gcaaatcgaa gtaaccttcg
     3661 acatcgacgc caacggcatc ctgcacgttt ccgccaaaga caaaggcacc ggtaaagcgg
     3721 ccaacatcac catccaaggt tcttcaggct tgagcgaaga agaaatcgaa cgcatggtga
     3781 aagatgccga agccaatgct gaggaagata aaaagctgac tgaattggtc gcttcccgca
     3841 accaagccga agccctgatt cactccgtga aaaaatcttt ggccgactac ggcgacaaac
     3901 tcgacgctgc cgagaaagaa aaaatcgaag ccgcgctgaa agaagccgaa gaagccgtga
     3961 aaggcgacga caaaaccgcc atcgatgcca aagccgaagc actgggcaca gccagccaaa
     4021 aactggggga aatggtttac gctcaagcac aagctgaagc ccaagcaggc gaaagcgaac
     4081 aagccaatgc ttctgcaaag aaagacgatg atgtcgtaga tgccgacttt gaagaagtaa
     4141 aagacgacaa aaaataattg atgccgtctg aaaaaagcgc gaaccgtttg attcgcgctt
     4201 ttttcaattg agataaaaga ccatagcata acagaggttc caaattcatc taccgtgata
     4261 ttcccaagcc caagttctaa ctgttatatc ggttctctgg aaatttttt
//
LOCUS       DAWHSK010000169         4308 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.169, whole genome shotgun sequence.
ACCESSION   DAWHSK010000169 DAWHSK010000000
VERSION     DAWHSK010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4308
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.169"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            29..1807
                     /locus_tag="WIC03_001601"
     CDS             29..1807
                     /locus_tag="WIC03_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/type III pantothenate kinase"
                     /protein_id="HEZ3884076.1"
                     /translation="MTVLKLSHWRVLAELADGLPQHVSQLARMADMKPQQLNGFWQQM
                     PAHIRGLLRQHDGYWRLVRPLAVFDAEGLRELGERSGFQTALKHECASSNDEILELAR
                     IAPDKAHKTICVTHLQSKGRGRQGRKWSHRLGECLMFSFGWVFDRPQYELGSLSPVAA
                     VACRRALSRLGLKTQIKWPNDLVVGRDKLGGILIETVRTGGKTVAIIGIGINFVLPKE
                     VENAASVQSLFQTASRRGNADAAVLLETLLAELDAVLLQYARDGFAPFVAEYQAANRD
                     HGKAVLLLRDGETVFEGTVKGVDGQGVLHLETAEGEQTVVSGEISLRPDDRPVSVPKR
                     RDSERFLLLDGGNSRLKWAWVENGTFATVGSAPYRDLSPLGAEWAEKADGNVRIVGCA
                     VCGEFKKAQVQEQLARKIEWLPSSAQALGIRNHYRHPEEHGSDRWFNALGSRRFSRNA
                     CVVVSCGTAVTVDALTDDGHYLGGTIMPGFHLMKESLAVRTANLNRHAGKRYPFPTTT
                     GNAVASGMMDAVCGSVMMMHGRLKEKTGAGKPVDVIITGGGAAKVAEALPPAFLAENT
                     VRVADNLVIHGLLNLIAAEGGESEHT"
     gene            1920..2744
                     /locus_tag="WIC03_001602"
     CDS             1920..2744
                     /locus_tag="WIC03_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEZ3884077.1"
                     /translation="MKWLFTLLAVLNIAVFGGTVGYKLAVKATGGVPENRAVENTPPA
                     TPAADNAAASVEDTAALLKPGDILTEEQAEQLRLKKEAEQKKLREKKQREEKARREKL
                     AAEKAQAERENGAADALCAAQASLTMDEDDYHRIKGLLGKWSHVASRSVEKRTAKAKP
                     ADKTYRVVLPVSADAENQAAELSAKGFNPIPFDGALSLGVGNSRENAQALQNRLAGAG
                     FGGAHIVEHFAEADRQDDSLSVSRMTVLFTGVNAADADEIRKITSLYGKLNLKSCK"
     gene            complement(3507..4295)
                     /locus_tag="WIC03_001603"
     CDS             complement(3507..4295)
                     /locus_tag="WIC03_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEZ3884078.1"
                     /translation="MLTLYGETFPSRLLLGTAAYPTPEILKQSVRIAQPAMITVSLRR
                     TGSGGEAHGQGFWSLLQETGVPVLPNTAGCQSVQEAVTTAQMAREVFETDWIKLELIG
                     DDDTLQPDVFQLVEAAEILIKDGFKVLPYCTEDLIACRRLLDVGCQALMPWAAPIGTG
                     LGAVHAYALNVLRERLPDTPLIIDAGLGLPSQAAQVMEWGFDGVLLNTAVSRSGDPVN
                     MARAFALAVESGRLAFEAEPVEARDKAQASTPTVGQPFWHSAEY"
BASE COUNT          944 a         1132 c         1346 g          886 t
ORIGIN      
        1 caaaaatccg tgcggcagag ggcggaaaat gacggttttg aagctttcgc actggcgggt
       61 gttggcggag cttgccgacg gtttgccgca acatgtctcg caactggcgc gtatggcgga
      121 tatgaagccg cagcagctca acggtttttg gcagcagatg ccggcgcaca tacgcgggct
      181 gttgcgccaa cacgacggct attggcggct ggtgcgccca ttggcggttt tcgatgccga
      241 aggtttgcgc gagctggggg aaaggtcggg ttttcagacg gcattgaagc acgagtgcgc
      301 gtccagcaac gacgagatac tggaattggc gcggattgcg ccggacaagg cgcacaaaac
      361 catatgtgtg acccacctgc aaagtaaggg cagggggcgg caggggcgga agtggtcgca
      421 ccgtttgggc gagtgtctga tgttcagttt tggctgggtg tttgaccggc cgcagtatga
      481 gttgggttcg ctgtcgcctg ttgcggcagt ggcgtgccgg cgcgccttgt cgcgtttggg
      541 tttgaaaacg caaatcaagt ggccaaacga tttggtcgtc ggacgcgaca aattgggcgg
      601 cattctgatt gaaacggtca ggacgggcgg caaaacggtt gccatcatcg gtatcggcat
      661 caatttcgtg ctgcccaagg aagtggaaaa cgccgcttcc gtgcaatcgc tgtttcagac
      721 ggcatcgcgg cggggaaatg ccgatgccgc cgtgttgctg gaaacgctgt tggcggaact
      781 tgatgcggtg ttgttgcaat atgcgcggga cggatttgcg ccttttgtgg cggaatatca
      841 ggctgccaac cgcgaccacg gcaaggcggt attgctgttg cgcgacggcg aaaccgtgtt
      901 tgaaggcacg gttaaaggtg tggacggaca aggcgttctg cacttggaaa cggcagaggg
      961 cgaacagacg gtcgtcagcg gcgaaatcag cctgcggccc gacgacaggc cggtttccgt
     1021 gccgaagcgg cgggattcgg aacgttttct gctgttggac ggcggcaaca gccggctcaa
     1081 gtgggcgtgg gtggaaaacg gcacgttcgc aaccgtcggt agcgcgccgt accgcgattt
     1141 gtcgcctttg ggcgcggagt gggcggaaaa ggcggatgga aatgtccgca tcgtcggttg
     1201 cgccgtgtgc ggagaattca aaaaggcaca agtgcaggaa cagctcgccc gaaaaatcga
     1261 gtggctgccg tcttccgcac aggctttggg catacgcaac cactaccgcc accccgaaga
     1321 acacggttcc gaccgctggt tcaacgcctt gggcagccgc cgcttcagcc gcaacgcctg
     1381 cgtcgtcgtc agttgcggca cggcggtaac ggttgacgcg ctcaccgatg acggacatta
     1441 tctcggggga accatcatgc ctggtttcca cctgatgaaa gaatcgctcg ccgtccgaac
     1501 cgccaacctc aaccggcacg ccggtaagcg ttatcctttc ccgactacaa cgggcaatgc
     1561 cgtcgccagc ggcatgatgg atgcggtttg cggctcggtt atgatgatgc acgggcgttt
     1621 gaaagaaaaa accggggcgg gcaagcctgt cgatgtcatc attaccggcg gcggcgcggc
     1681 aaaagttgcc gaagccctgc cgcctgcatt tttggcggaa aataccgtgc gcgtggcgga
     1741 caacctcgtc attcacgggc tgctgaacct gattgccgcc gaaggcgggg aatcggaaca
     1801 tacttaaaaa atacaccctt tttcaacacg gggcggtatc cgtccgaatc cgtccggatt
     1861 ctgccgcccg acagactgcc gcccaggcgg gcggggtttc aaacattgag gaaattctaa
     1921 tgaaatggct cttcaccctt ttggcggtac tcaatattgc cgtattcggc ggcacggtgg
     1981 gctataaatt ggccgttaaa gcgacgggcg gcgtgccgga aaaccgggcc gtcgaaaaca
     2041 cgccccccgc aacgcccgcc gccgacaatg cggcggcatc cgtggaggat acggcggcac
     2101 tgctcaaacc gggcgacatc ctgaccgaag agcaggccga gcagttgcgc ttgaaaaagg
     2161 aagcggaaca gaaaaaactg agggagaaaa aacagcgtga agaaaaagcc cgccgcgaaa
     2221 aactcgccgc cgaaaaggcg caggcggaac gcgaaaacgg cgcggcggat gccttatgcg
     2281 ccgcgcaggc aagcctcacg atggacgaag atgactacca ccgcatcaaa ggacttttgg
     2341 gcaaatggtc gcacgttgcc agcaggagcg tcgaaaaacg caccgccaaa gccaaacctg
     2401 ccgacaaaac ctaccgcgtc gtcctgcccg tttccgccga tgccgaaaat caggcggcgg
     2461 agctgtctgc caaaggtttc aaccccatac cgtttgacgg cgcattgagt ttgggtgtcg
     2521 gcaacagccg ggaaaacgcc caagccctgc aaaaccggct tgccggcgcc ggattcggcg
     2581 gggcgcatat tgtcgaacac tttgccgaag ccgacaggca ggacgattct ttgagcgtgt
     2641 cgcgtatgac ggttttgttt accggcgtga atgccgccga tgcggacgaa attcgtaaaa
     2701 tcacgtccct atacggcaaa ctgaacctca agtcttgcaa ataggcgcga aagccggacg
     2761 ataaacgcca aacccaccgc gaaggtgggt ttggcgtttc ggttttgagt atctgatata
     2821 taaaaaggga atgtatgtct cgtatatgcc gactccaagt gtgaaagtga tgatggggaa
     2881 atatggtgtt taatggttcg gaacggacga cttccgagtg gttttgatta tacgctaata
     2941 ataataatta tcaataaaaa tttaaaaata aatgcggatt ttttgtaatg agatgaaaaa
     3001 taaacaaatt aaactatttg ccgatgtttg tgattcgggt cggcaaactt tcattgtgcc
     3061 ggacaaatcg gggtcggcag gcatagctga gggcttcgtg cctgacggaa ataaggtcgc
     3121 ccgtgtttga acattcgtcc gatttggcgc ggacagggtt gttatttggg ggtgtttgag
     3181 cgtggattcg gggatgcgaa agcggtaaaa ccgttgcgga tgaaggctta cggacggctt
     3241 taggtatttt taagtgtatt caaacaacaa caaaacgctt cttctattgc cgtatttagg
     3301 gcttgcgcgg cagcctgcac accgtggagt ccgggtattt cgttggttgc ggcatcggga
     3361 cgggcggcaa ggttttccga ccggtttttc cattttttcc gacaaagccg tgatgccggg
     3421 gaatggcggt ttgcccgttt cggaccgccg ccgttttgag ccgcgatgcc gtctgaagcc
     3481 ttcagacggc atttttgccg cctttttcaa tattccgccg aatgccaaaa cggctgtccg
     3541 actgtcggcg tgctggcttg cgctttgtcg cgtgcttcga ccggttcggc ttcaaatgcc
     3601 agccgtccgg attcgacggc gagtgcgaag gcgcgtgcca tattgaccgg atcgccgctg
     3661 cgggaaacgg cagtattcaa aagcacgccg tcaaaacccc attccatcac ttgtgccgcc
     3721 tgtgagggca aacccaagcc cgcgtcgata atcagcggcg tgtcgggcag gcgttcgcgc
     3781 aggacgttca acgcgtaggc gtgaaccgcg cccaaacccg tgccgatcgg agccgcccac
     3841 ggcattaacg cctgacagcc cacatcgagc aggcggcggc aggcaatcag gtcttcggtg
     3901 caataaggca gcactttgaa gccgtcttta atcaggattt ccgccgcttc gacaagttgg
     3961 aacacatccg gctgcaaggt gtcgtcgtcg cctatgagtt cgagtttaat ccaatcggtt
     4021 tcaaacactt cgcgcgccat ttgcgccgtc gttaccgctt cctgcacgct ttggcagcct
     4081 gccgtgttcg gcaggacggg aacgccggtt tcttgaagca gcgaccaaaa cccctgaccg
     4141 tgcgcctcgc cgccgcttcc cgtgcggcgc agcgagacgg taatcatcgc gggttgggcg
     4201 atacggacgg attgtttgag gatttcaggg gtcgggtagg cagccgtgcc gagcagcagc
     4261 cgcgagggga aagtttctcc gtatagggtg agcataatgg gttccttt
//
LOCUS       DAWHSK010000170         4292 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.170, whole genome shotgun sequence.
ACCESSION   DAWHSK010000170 DAWHSK010000000
VERSION     DAWHSK010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4292
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.170"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(15..1029)
                     /locus_tag="WIC03_001604"
                     /pseudo
     CDS             complement(15..1029)
                     /locus_tag="WIC03_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273336.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(1185..1778)
                     /gene="ribA"
                     /locus_tag="WIC03_001605"
     CDS             complement(1185..1778)
                     /gene="ribA"
                     /locus_tag="WIC03_001605"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HEZ3884079.1"
                     /translation="MSELLNYVASCRLPTEWGVFTMHGFEEANGQEHVALTVGDFSDG
                     NPVLTRIHSECLTGDALFSRKCDCGPQLEAAMRAVQTEGRGIIVYLRQEGRGIGLINK
                     IRAYHLQDQGMDTVEANLALGLPVDARDFRLAQSIYEYLGIRSVKLLTNNPEKIQTLK
                     DAGINVVERIPLHVGENLENKRYLQTKADKLGHLMSE"
     gene            complement(1771..2445)
                     /locus_tag="WIC03_001606"
     CDS             complement(1771..2445)
                     /locus_tag="WIC03_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884080.1"
                     /translation="MPAALIKDFLLTQGLKLPLDEVRTAYLTAQTVMDMGTTSIERSV
                     LWLEHEDWRLADYLEQNSGNEEGLKRLFMALDSVFSRCKGVRAAAVYALMPSENAAFR
                     LVCLSRQGEVLENLWDLDEAAGKVSLACRSAQSGWMNVASDVRRWLDLGELSGERNHA
                     SAAQISIPVCTESGGVLGVVHVEFECAECAGTAAQVEWVALALALSEPLKQLLGITAA
                     EGDENV"
     gene            2599..2683
                     /locus_tag="WIC03_001607"
     tRNA            2599..2683
                     /locus_tag="WIC03_001607"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2633..2635,aa:Leu,seq:tag)
     gene            3203..4237
                     /gene="purM"
                     /locus_tag="WIC03_001608"
     CDS             3203..4237
                     /gene="purM"
                     /locus_tag="WIC03_001608"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEZ3884081.1"
                     /translation="MSTSLSYRDAGVDIDAGDQLVENIKPFAKRTMRPEVLGDLGGFG
                     ALVEIGKKYKNPVLVSGTDGVGTKLKLAFDWDKHDMVGIDLVAMSVNDILVQGAEPLF
                     FLDYFACGKLDVPRATDVIKGIAQGCEESGCALIGGETAEMPGMYPVGEYDLAGFAVG
                     VVEKENVITGRSIGAGDVVLGLASNGAHSNGYSLIRKIIERDNPDLDAEFDNGKTLRE
                     AVIAPTRLYVKPILAALEKFTIKGMAHITGGGITENVPRVLPENTVAQIDAKSWELPK
                     LFQWLQKAGNVETQEMYRTFNCGIGMVVIVAAEDADAVQGLLGEQGETVYRLGLIRER
                     QGDEHQTQVA"
BASE COUNT         1123 a         1093 c         1034 g         1042 t
ORIGIN      
        1 ggtttgccgg aagcttattc cgaaaaaaat ttctgataaa cggccttgat tgccgggtac
       61 agcataggcg gtacgagcag gcgcaatatc ggcaatccta tgatgaatgc ggtcttcccg
      121 ataaaaaccg gacggattgt cttcgcgccg aatatctttc tgcatttttc tttaaatgaa
      181 aaatatttcc ggtagatgag gataatgttt ttggaaaaat aaagccttac caatatagac
      241 ctcagatagc cgatattgtc gaagacaaat ttttgcaaac cgccgtagat gggggaattt
      301 ttgttttcga ctaaaaaatc cacaccccgg tcgagatgcg tcagcacatc gctgaaattt
      361 ttgtatttga tgttgtggct gatagagccg ccgagaacgc ggtactggta aaaagggtta
      421 tcgtaaaagg caaaagtctt gataaaacgc gccaattgca aactgtacgg gaaatcctcg
      481 tgaatgtatc cttttgggaa aaacagattg tttttgatga ttatttcccg cctgacgatt
      541 tttgtccacg cgttggcgat atagtaccgc ccctccacca gcgtttcaaa atggcggaca
      601 aaatcattat ccgaaaagtc cgcccctttg gggatgtcgc ggtaattgaa ggacgaggga
      661 tgcaggatca aatcaacctt tttgtctgca agttgttgta aatcaaagag aatccccccc
      721 ccccgcgttt tttgaacggt tggtatcggc ccaataatcg tcgctgtcca aaaagattag
      781 gtaatcgcct tttgccgccc ggataccggc gttgcgggca tccgacagcc cgccgttttc
      841 ttgatgaatc acttttatat gcggatattt gcctgcatat tcgtcgcaaa tcttcccgca
      901 gccgtccggc gaaccgtcat cgaccaaaat catttcataa tcggcaaaat tttcggcaag
      961 cacggaatcc acgcagcagc ggaggtattt ttccacattg taaataggga cgatgatgga
     1021 gaaaatcata aatatcaatc cattatatta agatgtttgc gcgtatgcct caaacccgcg
     1081 ctcgcaatgc gtttgcatcc actataaaac ttattgcttt atcaaggtat ggaaacctgt
     1141 ttcccgaaag gcggcgcagg atgcccgttc cctgcaactt tgccttattc cgacatcaga
     1201 tgtcccagct tgtctgcttt ggtttggagg tagcgtttgt tttcaaggtt ttccccgacg
     1261 tgcaggggga tgcgttcgac cacgttaatc cccgcatctt tcagggtttg gattttttcg
     1321 gggttgttgg tcagcagttt gaccgagcgg atgcccagat attcgtagat agattgcgcc
     1381 aaacggaaat cgcgggcatc gacgggcagc ccgagtgcca aattggcttc aaccgtatcc
     1441 ataccttggt cttgcagatg ataggcgcgg attttgttaa tcagcccgat gccgcgtcct
     1501 tcctgacgca gatagacgat gatgccgcgc ccctctgtct gtacggccct catggccgct
     1561 tcaagttgag gtccgcagtc gcattttctc gagaacagcg catcgcccgt caggcattcg
     1621 gagtggatgc gcgtcagaac cggattgccg tctgaaaaat caccgacggt cagcgcgacg
     1681 tgttcctgcc catttgcctc ttcaaagccg tgcatcgtaa atacgcccca ttcggtcggc
     1741 aggcggcagg aagcaacata gtttaataat tcagacattt tcatctcctt ctgcggcagt
     1801 gatgcccaaa agctgcttca aaggttcgga caaagccaag gcaagagcca cccattccac
     1861 ctgtgctgcc gtacccgcac actctgcgca ttcaaattcc acatgaacca cgcccaacac
     1921 accgccactt tccgtgcaga ccggaatgga aatttgcgcc gccgaagcat gattgcgttc
     1981 tcccgaaagc tctcccaaat ccaaccaacg gcgtacatcc gaggcaacat tcatccagcc
     2041 gctttgcgcc gaacggcaag ccagcgaaac cttgcctgcc gcttcatcca aatcccacag
     2101 gttttccaaa acctcgccct gccgggacag gcaaaccagc cgaaaggcgg cattttcaga
     2161 cggcatcagg gcataaacgg ctgcggcgcg cacgccttta cagcgtgaaa acaccgaatc
     2221 caaagccatg aaaagccgtt tcagaccttc ctcattcccg ctgttttgct ccaaataatc
     2281 ggcaagcctc caatcctcat gctcaagcca caaaaccgaa cgctcaatcg aagtcgtccc
     2341 catatccatt accgtctgcg ccgtcagata cgccgtccga acctcgtcaa gcggcagctt
     2401 caaaccctga gtcagcagaa aatccttaat caaagcagca ggcataccca aattccgtca
     2461 ataaaaacaa aaaaccgccc gattccgatg tcaggcggac gaaaaacaga ttttaccgcc
     2521 ccaaacccca agccatcaac agccccgccg aaaaccttgc acaaaaaaac aaaatctgta
     2581 taattgtcca attcccatgc ggacgtggcg aaattggtag acgcaccaga tttaggttct
     2641 ggcgccgaga ggtgtgagag ttcgagtctc tccgtccgca ccaataatga taaataaatc
     2701 atatagttaa tgcttatcgg gacaaattag tgccacaaaa cataagtcgt ctgcttttca
     2761 gcgggcggct tttttatctg cctatatgtt tcaacacgca gaacggtaca taaagtgccg
     2821 ccatttgttc taccccgaat cagaagcggg tgcttccgcc cgcaatcaga ttcaccgccc
     2881 ctaatgccgg ggtttcaact ggaaagaaga aaatattgat tttgatgatg gcatcaaaaa
     2941 accccttccg cccgtctgcc aaaaggaatg gttaagaaaa gcaatgcgtc ttttgatgga
     3001 acgcgatata taaggaattg aggggaaggg caggcgggtt tctgcccgtt aatcctgcgg
     3061 acgatcccgc caaacccgac acgcgcgggc atatggcgga ttgtttacaa tattgtctaa
     3121 aaatctgaac ctgcaaacgt gaaataaggt agaatacgcc ccgttatttt accgtccaat
     3181 ccccatttac caaggaaaaa cgatgagtac ttcattgagt taccgcgatg caggtgtcga
     3241 tatcgacgca ggcgaccaac tggtcgaaaa catcaaaccg tttgccaaac gcaccatgcg
     3301 tccagaagta ttgggggatt tgggcggttt tggcgcattg gtcgaaatcg gcaagaaata
     3361 taaaaatccc gtattggtca gcggtacgga cggtgtgggt accaagctca agcttgcctt
     3421 tgattgggat aaacacgata tggtgggcat cgaccttgtt gcaatgagtg tcaacgatat
     3481 tttggttcaa ggagcagaac ctctgttttt cttggattat ttcgcctgcg gcaaattgga
     3541 tgttccgcgc gcaaccgatg tcatcaaagg cattgcacaa ggttgcgaag aatccggctg
     3601 cgccctgatt ggcggggaaa ctgccgaaat gccgggcatg tatcccgtcg gagaatacga
     3661 tttggcgggt tttgccgtcg gcgtagtgga aaaagagaat gtcattaccg gccgcagtat
     3721 cggcgcgggc gatgtggtat tgggtttggc ttccaacggc gcacattcaa acggctattc
     3781 ccttatccgt aaaatcatcg aacgcgacaa tcccgatttg gatgccgagt ttgataatgg
     3841 caaaaccttg cgggaggctg ttattgcgcc gacccgtctg tatgtgaaac cgattcttgc
     3901 cgctttggaa aaatttacca ttaaaggtat ggcacacatt accggcggcg gcattaccga
     3961 aaacgtgccg cgcgtgttgc ctgaaaacac ggttgcacaa atcgatgcta aatcgtggga
     4021 attgcccaag ctcttccaat ggcttcaaaa ggcgggcaat gtggaaaccc aagaaatgta
     4081 ccgaaccttt aactgcggca tcggcatggt cgttattgtt gctgccgaag atgccgatgc
     4141 ggttcagggt ctcttgggtg aacaaggtga aacagtttac cgtttaggtt tgatccgtga
     4201 gcgtcaggga gacgagcatc aaacccaggt tgcctgattg cttctatagc gaaatgccgt
     4261 ctcaaacatg ggtttggacg gcatttttat ag
//
LOCUS       DAWHSK010000171         4261 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.171, whole genome shotgun sequence.
ACCESSION   DAWHSK010000171 DAWHSK010000000
VERSION     DAWHSK010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4261
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.171"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            538..2265
                     /gene="ilvB"
                     /locus_tag="WIC03_001609"
     CDS             538..2265
                     /gene="ilvB"
                     /locus_tag="WIC03_001609"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HEZ3884082.1"
                     /translation="MQLSGAQIIVQSLKAEGVEYVFGYPGGAVIEIYDALFQLNKFKH
                     ILTRHEQAAVHAADAYARVSGKVGVALVTSGPGVTNALTGIATAYTDSIPMVVISGQV
                     GNSLIGTDAFQEVDTVGITRPCVKHNFLVTDINELAETIKKAFQIAASGRPGPVVVDV
                     PKDVTQAMAKFSYPQEDIFIRSYQPVVQGHIGQIKKAVQMLASAKRPVVYFGGGVVLG
                     NASEELTRFVRMTGAPCTGTLMGLGAYPSGDRQFLGMLGMHGTYEANLAMQNADVVLA
                     VGARFDDRVVSVPSKFFEKAKKVIHIDVDPSSIAKRVKADIPIVGDVKNVLSEMVALW
                     QKQESVPSEDALGKWWKTIEEWRSRDCLWFDNGSEIIKPQYVIQKLAEITGNSAIITS
                     DVGQHQMFAAQYYPFERPRQWLNSGGLGTMGVGLPYAIGAKLAAPDQDVFCITGDGSI
                     QMNIQELSTCFQYRIPVNVITLNNGYLGMVRQWQEIYYGGRESETYFDSLPDFVKLAE
                     AYGHIGIRVDKKSDVEGALLEALNQKDRLVFIDFLTDQKQNVMPMVGNGKGLDEMVLP
                     PHMRADEKA"
     gene            2276..2767
                     /gene="ilvN"
                     /locus_tag="WIC03_001610"
     CDS             2276..2767
                     /gene="ilvN"
                     /locus_tag="WIC03_001610"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEZ3884083.1"
                     /translation="MRHILSVLIENESGAMSRVVGLFSARDYNIDSLAVAPTEDKTLS
                     RMTIVTHGDEQVIEQITKQLNKLIEVIKVVDLNESRFVERELMLVKVRAAGKDRDEFL
                     RLTEIYRGSIIDVTDRSYTIEITGSTDKLDSFLETVGRAQILETVRTGAAGIGRGERI
                     LKI"
     gene            2834..3127
                     /locus_tag="WIC03_001611"
     CDS             2834..3127
                     /locus_tag="WIC03_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEZ3884084.1"
                     /translation="MSNIKIVALVTVKPEYTETLAAQFKELVKASRAEEGNISYDLHQ
                     EIGKPNRFVFVENWKSQAAIDEHNASAHFQAFVQSVDGKTEALEIVLMNEVAD"
     gene            3207..4220
                     /gene="ilvC"
                     /locus_tag="WIC03_001612"
     CDS             3207..4220
                     /gene="ilvC"
                     /locus_tag="WIC03_001612"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEZ3884085.1"
                     /translation="MQVYYDKDADLSLIKGKTVAIIGYGSQGHAHAANLKDSGVNVVI
                     GLRQGSSWKKAEAAGHVVKTVAEATKEADVVMLLLPDETMPAVYHAEVTANLKEGATL
                     AFAHGFNVHYNQIVPRADLDVIMVAPKGPGHTVRSEYKRGGGVPSLIAVYQDNSGKAK
                     DIALSYAAANGGTKGGVIETTFREETETDLFGEQAVLCGGVVELIKAGFETLTEAGYA
                     PEMAYFECLHEMKLIVDLIFEGGIANMNYSISNNAEYGEYVTGPEVVNASSKEAMRNA
                     LKRIQTGEYAKMFIQEGNVNYASMTARRRLNADHQVEKVGAQLRAMMPWITANKLVDQ
                     DKN"
BASE COUNT         1107 a         1014 c         1078 g         1062 t
ORIGIN      
        1 atcaatatat taagtcgttg ttagccctct tggttctgtc aatgccgtcc aatacagtcc
       61 ggtgcaatga gccattttca ggaagtatgg ttgggaatat ttttgatata tcaaaaataa
      121 aatcaagttc aatcaatatc ccgagtcttt attgggatga aaccatgtaa attttacaat
      181 tcttgatatc gaagaagggt ttttcacatc tgcccctcct gcataaactg tcaacaattt
      241 ccatctttca aaaaaggtac tcctgatttt attcttttaa aatcaatcaa taatttctct
      301 gctttcaaaa aatattaaaa gatgaaagat ttttcttgat tgaactgtcg gtttgcgtta
      361 tcgtttctac tctttttcac aaactctgtg ttcctttcca attgattgga taatccgtct
      421 gttgccgtgt tcccccaatg cgcgtaccct aaatcgctgc ttggtggaat tgcattacag
      481 gtgctgtggc aaagcggttt gtccctattg gtttgaaacc gtataaaaga ggtcgttatg
      541 cagttatcag gcgcgcaaat catagtgcag agtctcaaag ccgaaggtgt cgagtacgtt
      601 ttcggttatc ctggcggtgc ggttatcgaa atctacgatg cccttttcca actcaataaa
      661 ttcaagcaca ttctgacccg tcacgagcag gcggcagtac acgcggcaga tgcgtatgcg
      721 cgcgtcagcg gtaaggtggg cgtggcattg gttacatccg gcccgggcgt taccaatgca
      781 ctgaccggta ttgctactgc ctatacggat tcgattccga tggtggtcat cagcgggcag
      841 gtaggcaatt ccctgattgg tacggatgcg ttccaagaag ttgatacggt gggtattacc
      901 cgtccgtgcg tcaaacacaa tttcctggtt acggatatca atgagttggc ggaaaccatt
      961 aaaaaggcgt tccaaattgc cgcaagcggc cgaccagggc ctgtggtggt tgatgtcccg
     1021 aaagatgtta cgcaggcgat ggcgaaattc agctatcctc aggaagacat ttttatccgt
     1081 tcgtatcaac cggttgttca agggcatatc ggtcagatta aaaaggccgt gcagatgttg
     1141 gcatctgcca aacgtccggt cgtctatttt ggcggcggcg tggtgttggg taatgcttct
     1201 gaagagctga cccgatttgt ccgaatgacg ggtgctccgt gcacgggtac gctgatgggc
     1261 ttgggcgctt atccttccgg cgaccgccaa ttcctcggta tgctcggtat gcacggtact
     1321 tacgaggcaa accttgccat gcagaatgcg gatgtcgttc ttgccgtagg tgcgcgtttt
     1381 gacgaccgtg tggtttccgt accgtccaaa tttttcgaga aggcgaagaa ggttatccat
     1441 atcgatgtcg atccttccag catcgccaaa cgcgtgaagg cggacattcc gattgtcggc
     1501 gacgtgaaaa atgttttgtc cgagatggtt gcgctgtggc aaaaacaaga gtccgtgccg
     1561 tctgaagatg ctttgggcaa atggtggaaa accatagagg aatggcgttc ccgagattgc
     1621 ttgtggtttg acaacggcag cgaaattatc aagccacaat atgtgattca gaagcttgcc
     1681 gagattaccg gcaattcggc aatcatcaca tcggatgtag ggcagcatca aatgtttgcg
     1741 gctcaatatt atcccttcga acgtccgcgc caatggctca actccggcgg tttgggtacg
     1801 atgggcgtgg gtctgcctta tgcgattggt gcaaaacttg ccgccccgga tcaagacgta
     1861 ttctgtatta ccggcgacgg ctcgattcag atgaacatcc aagagttgtc cacctgtttc
     1921 caatatcgga ttccggttaa cgtcattacg ctgaacaacg gttatctcgg tatggtacgc
     1981 cagtggcagg aaatatatta cggcggccga gagtcggaaa cttatttcga ttctttgccc
     2041 gatttcgtca aacttgccga ggcatacggc catatcggta tccgcgtgga caagaagtct
     2101 gatgtggaag gtgcgttgtt ggaagcattg aaccaaaaag acaggctggt gtttatcgac
     2161 ttcctgaccg accagaaaca gaatgtgatg cccatggtcg gcaacggcaa aggtttggac
     2221 gaaatggtac ttccgccgca tatgcgtgcg gacgaaaagg cgtaaggaga gacaaatgcg
     2281 acatatctta tctgttctga ttgaaaacga atcaggtgcg atgagccgcg tggtcggttt
     2341 gttctctgca cgcgattaca atatcgattc tttggcggtt gcgccgaccg aagacaaaac
     2401 cctgtcccgt atgaccatcg ttacccacgg cgacgagcag gttatcgaac aaatcaccaa
     2461 gcaactcaat aaattgattg aagtgattaa agtggtcgat ttgaacgaaa gccgttttgt
     2521 cgaacgcgaa ctgatgttgg taaaagtccg tgccgccggc aaagaccgcg acgaattttt
     2581 gcgtttgacc gaaatctacc ggggcagcat catcgacgta accgaccgca gctatacgat
     2641 tgaaattacc ggctcgaccg acaagctgga ttcctttttg gaaacggtcg gacgcgccca
     2701 aattttggaa accgtacgta caggcgcggc cggcatcggt cgcggtgagc gtattttgaa
     2761 aatttaacgc cgtaaccttt cagacggcac ggtatttgaa tgccgtctga aaaacgaacg
     2821 gcaggagaga tttatgtcga acattaaaat tgtcgcactg gttaccgtca aaccggaata
     2881 cacagaaaca ctggcagcac agtttaaaga actggtcaaa gccagccgtg cagaagaggg
     2941 caacatcagc tacgatctcc atcaggaaat cggcaaaccg aaccgttttg ttttcgtgga
     3001 aaattggaaa tctcaagcag ctattgacga acataacgcc agcgcgcatt tccaagcctt
     3061 cgtccagtcc gtcgacggca aaaccgaagc gttggaaatc gtattgatga atgaagttgc
     3121 cgattaagcg gcacactctg tttaactcgt ccgaagccgc tcaacatttt aaggcttcga
     3181 caaccattta ccttaaagga aatcaaatgc aagtctatta cgataaagat gccgatctgt
     3241 ccctaatcaa aggcaaaacc gttgccatca tcggttacgg ttcgcaaggt catgcccatg
     3301 ctgccaacct gaaagattcg ggtgtaaacg tggtgatcgg tctgcgccaa ggttcttctt
     3361 ggaaaaaagc cgaagcagcc ggccatgtcg tcaaaaccgt tgctgaagcg accaaagaag
     3421 ccgatgtcgt tatgctgctg ctgcctgacg aaaccatgcc tgccgtctat cacgccgaag
     3481 ttacagccaa tttgaaagaa ggcgcaacgc tggcatttgc acacggcttc aacgtgcact
     3541 acaaccaaat cgttccgcgt gccgacttgg acgtgattat ggttgccccc aaaggtccgg
     3601 gtcataccgt acgcagtgaa tacaaacgcg gcggcggcgt gccttctctg attgccgttt
     3661 accaagacaa ttccggcaaa gccaaagaca tcgccctgtc ttatgcggct gccaacggcg
     3721 gcaccaaagg cggtgtgatt gaaaccactt tccgcgaaga aaccgaaacc gatctgttcg
     3781 gcgaacaagc cgtattgtgc ggcggcgtgg tcgagttgat caaggcgggt tttgaaaccc
     3841 tgaccgaagc cggttacgcg cctgaaatgg cttacttcga atgtctgcac gaaatgaaac
     3901 tgatcgttga cctgattttc gaaggcggta ttgccaatat gaactactcc atttccaaca
     3961 atgcggagta cggcgaatac gttaccggcc ctgaagtggt caatgcttcc agcaaagaag
     4021 ccatgcgcaa tgccctgaaa cgcattcaaa ccggcgaata cgcaaaaatg tttatccaag
     4081 agggtaatgt caactacgcg tctatgactg cccgccgccg tctgaatgcc gaccaccaag
     4141 ttgaaaaagt cggcgcacaa ctgcgtgcca tgatgccttg gattactgcc aacaaattgg
     4201 ttgaccaaga taaaaactga ttgttttcaa acgggactgc ctacacatcg tgtaggcagt
     4261 t
//
LOCUS       DAWHSK010000172         4233 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.172, whole genome shotgun sequence.
ACCESSION   DAWHSK010000172 DAWHSK010000000
VERSION     DAWHSK010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4233
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.172"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..769
                     /gene="gltS"
                     /locus_tag="WIC03_001613"
     CDS             <1..769
                     /gene="gltS"
                     /locus_tag="WIC03_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HEZ3884086.1"
                     /translation="ETQYGLVGATGLGIASATFGLVFGGLIGGPVARRLINKMGRKPV
                     ENKKQDQDDNADDVFEQAKRTRLITAESAVETLAMFAACLAFAEIMDGFDKEYLFDLP
                     KFVWCLFGGVVIRNILTAAFKVNMFDRAIDVFGNASLSLFLAMALLNLKLWELTGLAG
                     PVTVILAVQTVVMVLYATFVTYVFMGRDYDAAVLAAGHCGFGLGATPTAVANMQSVTH
                     TFGASHKAFLIVPMVGAFFVDLINAAILTGFVNFFKG"
     gene            868..1884
                     /locus_tag="WIC03_001614"
     CDS             868..1884
                     /locus_tag="WIC03_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HEZ3884087.1"
                     /translation="MYRKLIALPFALLLAACGREEPPKALECANPAVLQGIRGNIQET
                     LTQEARSFAREDGRQFVDADKIIAAAYGLAFSLEHASETQEGGRTFCIADLNITVPSE
                     TLADAKANSPLLYGETALSDIVRQKTGGNVEFKDGVLTAAVRFLPVKDGQTAFVDNTV
                     GMAAQTLSVALLPYGVKSIVMIDGKAVKKEDAVRILSGKAREEEPSKPTPEDILEHNA
                     AGGDAGVPQAAEGAPEPEILHPDDGERADTVTVSRGEVEEARVQNQRAESEITKLWGG
                     LDTDVQKELVGEQRKWAQEKISNCRQAAAQADRQEYAEYLKLQCDTRMTRERIQYLRG
                     YSID"
     gene            1895..2092
                     /locus_tag="WIC03_001615"
     CDS             1895..2092
                     /locus_tag="WIC03_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884088.1"
                     /translation="MTTVPKSRIPVKPLPEKTTDEAKVEKWRQLGAEHGLSGEWAVAA
                     RLGENGFTEEQMENIANLFGR"
     gene            2110..2259
                     /locus_tag="WIC03_001616"
     CDS             2110..2259
                     /locus_tag="WIC03_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884089.1"
                     /translation="MPSETLLSVSDGILTNTVRRRKTAVFSCVSYADVSTHRTIQKAS
                     PYVPS"
     gene            3832..4125
                     /locus_tag="WIC03_001617"
     CDS             3832..4125
                     /locus_tag="WIC03_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884090.1"
                     /translation="MKTNDKLNRQIDVLQKQSAAIHNEAYIEMNTLLYRHREVVSVHN
                     RKADYAEKGKEQIALFPRGLNGITKLPAAVLLPERPYHFDMKEVLHIFSWIPR"
BASE COUNT         1045 a         1139 c         1111 g          938 t
ORIGIN      
        1 tgaaacgcaa tacggcttgg tcggcgcaac cggtttgggt attgcatcgg ctactttcgg
       61 gctggtgttc ggcggcctga tcggtgggcc ggttgcgcgc cgcctgatca acaaaatggg
      121 ccgcaaaccg gttgaaaaca aaaaacagga tcaggacgac aacgcggacg acgtgttcga
      181 gcaggcaaaa cgcacccgcc tgattacggc ggaatctgcc gttgaaacgc ttgccatgtt
      241 tgccgcgtgt ttggcgtttg ccgagattat ggacggcttc gacaaagaat atctgttcga
      301 cctgcccaaa ttcgtgtggt gtctgtttgg cggcgtggtc atccgcaaca tcctcactgc
      361 cgcattcaag gtcaatatgt tcgaccgcgc catcgatgtg ttcggcaatg cttcgctttc
      421 gcttttcttg gcaatggcgt tgctgaattt gaaactgtgg gagctgaccg gtttggcggg
      481 gcctgtaacc gtgattcttg ccgtacaaac cgtggtgatg gttttgtacg cgacttttgt
      541 tacctatgtc tttatggggc gcgactatga tgcggcagta ttggctgccg gccattgcgg
      601 tttcggcttg ggtgcaacgc cgacggcggt ggcaaatatg cagtccgtca cgcatacttt
      661 cggcgcgtcg cataaggcgt ttttgattgt gcctatggtc ggcgcgttct tcgtcgattt
      721 gattaatgcc gcgattctca ccggttttgt gaatttcttt aaaggctgat tttccgcctt
      781 tccgacaaag cacctgcaag gtttaccgcc tgcaggtgct tttgctatga tagccgctat
      841 cggtctgcac cgtttggaag gaacatcatg tatcggaaac tcattgcgct gccgtttgcc
      901 ctgctgcttg ccgcttgcgg cagggaagaa ccgcccaagg cattggaatg cgccaacccc
      961 gccgtgttgc aaggcatacg cggcaatatt caggaaacgc tcacgcagga agcgcgttct
     1021 ttcgcgcgcg aagacggcag gcagtttgtc gatgccgaca aaattatcgc cgccgcctac
     1081 ggtttggcgt tttctttgga acacgcttcg gaaacgcagg aaggcgggcg cacgttctgt
     1141 atcgccgatt tgaacattac cgtgccgtct gaaacgcttg ccgatgccaa ggcaaacagc
     1201 cccctgctgt acggggaaac cgctttgtcg gatattgtgc ggcagaagac gggcggcaat
     1261 gtcgagttta aagacggcgt attgacggca gccgtccgct tcctgcccgt caaagacggt
     1321 cagacggcat ttgtcgacaa cacggtcggt atggcggcgc aaacgctgtc tgtcgcgttg
     1381 ctgccttacg gcgtgaagag catcgtgatg atagacggca aggcggtgaa aaaagaagac
     1441 gcggtcagga ttttgagcgg aaaagcccgt gaagaagaac cgtccaaacc cacgcccgaa
     1501 gacattttgg aacacaatgc cgccggcggc gatgcgggcg taccccaagc cgcagaaggc
     1561 gcgcccgaac cggaaatcct gcatcctgac gacggcgagc gtgccgatac cgttaccgta
     1621 tcacggggcg aagtggaaga ggcgcgcgta caaaaccagc gtgcggaatc cgaaattacc
     1681 aaactttggg gaggactcga taccgacgtg caaaaagagt tggtcggcga acaacgcaag
     1741 tgggcgcagg aaaaaatcag caactgccga caagccgccg cgcaggcaga ccggcaggaa
     1801 tacgccgaat acctcaagct gcaatgcgac acgcggatga cgcgcgaacg gatacagtat
     1861 cttcgcggct attccatcga ttaggggcaa accgatgact accgtcccca aaagcaggat
     1921 tcccgtcaaa ccgctgcccg aaaaaaccac agacgaagcc aaagtcgaaa aatggcggca
     1981 gctcggtgcg gaacacggtt tgtcgggcga atgggcagtt gccgccagat tgggcgagaa
     2041 cggttttacc gaagaacaga tggaaaatat cgccaacctg ttcggcagat aaagggaaaa
     2101 ttgacggaaa tgccgtctga aaccctgtta tcggtttcag acggcatttt gactaatacg
     2161 gtacgcaggc gcaaaacagc cgtcttttcc tgcgtttcct atgctgatgt ttcaacacac
     2221 aggacgatac aaaaagcgtc gccctatgtt ccgtcctaat ttttgttaat ccgctctaaa
     2281 gtctgatcct gccgccctaa agggtgggat ttcaaccgaa aaggaaatac gatagaatcg
     2341 gcaatttgcc gtaaagttaa aaagttccat catatcgagg agacattatg catcaccccc
     2401 cccccgcaaa atgaacattt cgagcaagac cttatccctt acgccgtccg tttcaggctc
     2461 aatcaaatcc ttcccgaact cgactgccat tggcaggaaa atctgacaga aatcttcaaa
     2521 acactttctc ccgaagccgg gcaggccgcc tgtacgcaaa tcctttcccg aaaaggcata
     2581 ggctataacg aagccgacgg ttcttttact taccttcccc aaaaacgcat ttcgttttct
     2641 tctttggcaa cagacattcc catccccgac ctcaaacggc tggcggggca tattgcggaa
     2701 cgcctggatg tattggcgca atgcgaatcg ccggtttata ttgccgacat tctcgaaagc
     2761 gtgctgtccg ttatcaaaaa cgtcgatacc ggaggcaagc gcgcacggga aaatttcaaa
     2821 cagattctga aaaccgaatt catctacgcc gccgctgcgg tattgaaaaa caaaaccaaa
     2881 tgggaaatcc ccgaaaatac ccgcaatctc gattcggaca tcatctgtac ctatatctgc
     2941 gaagtgttcc taaaatcgca cctgctcggc aacagttttc cgatgatgcg cccgcgcgaa
     3001 gtgaagcaga tgcccgaacc ggtgtttgac aaagtattgt ttgccgaaca gcgcgcccgc
     3061 cagttggaag ttatccgaac gtcaaaatac atcttcgcca tcgcgcctta ttacaccgac
     3121 gatattccgt tttccctgcg ccgcttcctg tcggaagaca aactttatac cagctacaac
     3181 cgctattaca ccgccatcca tatccccgtc cgcaacatca ctcagaatga tgccctgcgt
     3241 tttgccaacg gtttgaaaca gattctctcg ctgcaatccg gcgtgagctg ggaaatcatc
     3301 gatatgatgg acaggataga ggaaaactac gacaaaaaag ccctgccgct gttgttttcg
     3361 ccattccccg cgcaagtcga acgcacgcag gcgattgccg ccagattgga ccaattcgag
     3421 cggctgttgg gcgacaccgt cctcgatccg ttctactatt gcctgacgcg tatggcgaag
     3481 agcgaggaag atttgaaata tatttatatc gccttccgcc agtctttcgg cggcatcttc
     3541 aattcgtttg aaaacttccg cctgctgccc gcgctgtgga tgagcaggga tgcggaaaat
     3601 atgtccgacc gcatgaacgc ctatatttcc gcaatggaag accgccgccg cgagatattg
     3661 tccattcggc agggaaagcc tgaagaagaa ttttcccgca agatggtcgt ctgcctgatc
     3721 gacttggaaa attgtttgga aaaacacctc gaacagttca agccggtatc ggaaagcatc
     3781 gaagcctgta ccgccaaact gcaggagcaa ccgtcgttct tcaacaggct gatgaaaacc
     3841 aacgacaagc tcaacaggca gattgacgtg ttgcagaaac agtcggcagc catccacaac
     3901 gaagcctata tcgaaatgaa cacgctgctt taccgccacc gcgaagtggt ttccgtccac
     3961 aaccgcaaag cagattatgc ggaaaagggc aaagagcaga ttgcgctgtt cccgcgcgga
     4021 ttgaacggca tcaccaagct gcccgccgcc gtcctgttgc ccgagcgtcc ctaccatttt
     4081 gatatgaagg aggttctgca tatattctcc tggataccga ggtaggcgta tcggctgccg
     4141 gataaaaatg caccccgtca ttcctgtctg tacgggggta gcggcagtgt tttcctgctt
     4201 cgttttatag tggattaaca aaaaccagta cgg
//
LOCUS       DAWHSK010000173         4218 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.173, whole genome shotgun sequence.
ACCESSION   DAWHSK010000173 DAWHSK010000000
VERSION     DAWHSK010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4218
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.173"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            41..454
                     /locus_tag="WIC03_001618"
     CDS             41..454
                     /locus_tag="WIC03_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEZ3884091.1"
                     /translation="MMPAYAYAALAFSAFTSATLLPGTSEAAFALFVHRFPEHAYGAL
                     LCAGLANGLGSMVSYWMGRLLPSRKMPSEKTLNLMRRFGIWLLAFAWLPVVGDALPLA
                     AGWLRLNPWTSGLMLVIGKTARYAFILWGMQYYAA"
     gene            500..1129
                     /gene="nth"
                     /locus_tag="WIC03_001619"
     CDS             500..1129
                     /gene="nth"
                     /locus_tag="WIC03_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEZ3884092.1"
                     /translation="MNRHIRQEIFERFRAANPHPTTELNFNSPFELLIAVLLSAQATD
                     VGVNKATAKLFPVADTPQAMLDLGLDGVMEYTKTIGLYKTKSKHIMQTCRILLEKYNG
                     EVPEDREALESLPGVGRKTANVVLNTAFGHPVMAVDTHIFRVSNRTKIAPGKDVREVE
                     DKLMRFIPKEFLMDAHHWLILHGRYTCKALKPQCQTCIINDLCEYPAKA"
     gene            1267..2766
                     /locus_tag="WIC03_001620"
     CDS             1267..2766
                     /locus_tag="WIC03_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEZ3884093.1"
                     /translation="MFKKYQYLALAALCAASLAGCDKAGSFFGADKKEASFVERIEHT
                     KDDGSVSMLLPDFVQLVQSEGPAVVNIQAAPAPRTQNGSGNAETDSDPLADSDPFYEF
                     FKRLVPNMPEIPQEEADDGGLNFGSGFIISKDGYILTNTHVVTGMGSIKVLLNDKREY
                     TAKLIGSDVQSDVALLKIDATEELPVVKIGNPKDLKPGEWVAAIGAPFGFDNSVTAGI
                     VSAKGRSLPNESYTPFIQTDVAINPGNSGGPLFNLKGQVVGINSQIYSRSGGFMGISF
                     AIPIDVAMNVAEQLKNTGKVQRGQLGVIIQEVSYGLAQSFGLDKAGGALIAKILPGSP
                     AERAGLQAGDIVLSLDGGEIRSSGDLPVMVGAITPGKEVSLGVWRKGKEITVAVKLGN
                     ASEQTGSSSEPDKAPYAEHQSGTFSVESAGITLQTHTDSSGGRLVVVRVSGAAERAGL
                     RRGDEILAVGQVPVNDEDGFRKAMDKAGKNVPLLVMRRGNTLFIALNLQ"
     gene            2969..>4218
                     /locus_tag="WIC03_001621"
     CDS             2969..>4218
                     /locus_tag="WIC03_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HEZ3884094.1"
                     /translation="MQLIDYSHSFFSVVPPFLALALAVITRRVLLSLGIGILVGVAFL
                     VGGNPVDGLTHLKDMVVGLAWSDGDWSLGKPKILVFLILLGIFTSLLTYSGSNQAFAD
                     WAKRHIKNRRGAKMLTACLVFVTFIDDYFHSLAVGAIARPVTDKFKVSRAKLAYILDS
                     TAAPMCVLMPVSSWGASIIATLAGLLVTYKITEYTPMGTFVAMSLMNYYALFALIMVF
                     VVAWFSFDIGSMARFEQAALNEAHDETAASDATKGRVYALIIPVLALIASTVSAMIYT
                     GAQASETFSILGAFENTDVNTSLVFGGTCGVLAVVLCTLGTIKTADYPKAVWQGAKSM
                     FGAIAILILAWLISTVVGEMHTGDYLSTLVAGNIHPGFLPVILFLLASVMAFATGTSW
                     GTFGIMLPIAAAMAVKVEPALIIP"
BASE COUNT          977 a         1277 c         1051 g          913 t
ORIGIN      
        1 caatccgtcg ccgatttttg cctgtttgag actgtttgca atgatgcccg cctacgccta
       61 cgccgcgctc gctttttccg ccttcacttc cgccaccctc ctgcccggca catccgaagc
      121 cgcctttgcc ctgttcgtcc accgctttcc cgaacacgca tacggcgcgt tgctgtgcgc
      181 cggccttgcc aacggattgg gcagtatggt ttcctattgg atggggcgtt tgctgccctc
      241 ccgaaaaatg ccgtctgaaa aaacactgaa tctgatgcgg cgtttcggta tttggctgct
      301 tgcgtttgcc tggctgcccg tcgtcggcga cgcactgccg cttgccgccg gctggctgcg
      361 gttgaatccg tggacaagcg ggctgatgct ggttatcggc aaaacggcgc gttacgcctt
      421 cattctgtgg ggaatgcaat attacgccgc ctaaaccata cgttataatg ccgaacacac
      481 aaacccaccg tataaaaaaa tgaacagaca catccgccaa gaaatcttcg aacgcttccg
      541 cgccgccaac ccccatccga ccacagaact caacttcaac tcccctttcg agcttttaat
      601 cgccgtttta ctgtcggcac aggcaaccga cgtcggcgta aacaaagcga cggcgaagct
      661 gtttccggtt gccgatacgc cgcaggcgat gctggatttg ggtttggacg gcgtgatgga
      721 atatacgaaa accatcgggt tgtataaaac caagtccaag cacatcatgc agacctgccg
      781 catcctgctg gaaaaataca atggcgaagt gccggaagac cgcgaggctt tggaatcgtt
      841 gccgggcgtg gggcgcaaaa cggcaaacgt ggtattgaac acagcgttcg gacaccccgt
      901 tatggcggtc gatacgcata ttttccgcgt gtccaaccga accaaaatcg cccccgggaa
      961 agatgtgcgc gaagtcgaag acaaactgat gcgcttcatt cctaaagaat ttctgatgga
     1021 cgcgcaccac tggctgattt tgcacggacg ctacacctgc aaggcactca aaccgcaatg
     1081 ccaaacctgc atcatcaacg atttgtgcga atatcccgcc aaagcctgac cgccgccgcc
     1141 cttcaaaatg aaacgcttga aaattcggac ggacgttgta gaatttcaac ctatgccgtc
     1201 tgaagccgac aaaatgcgct tcagacggca ttaaagccgg taccggcatc cgacaaagga
     1261 caaaacgtgt tcaaaaaata ccaatacctc gctttggcag cactgtgtgc cgcctcgctg
     1321 gcaggctgcg acaaagccgg cagctttttc ggtgcggaca aaaaagaagc atccttcgta
     1381 gaacgcatcg aacacaccaa agacgacggc agcgtcagta tgctgctgcc cgactttgtc
     1441 caactggttc aaagcgaagg cccggcagtc gtcaatattc aggcagcccc cgccccgcgc
     1501 acccaaaacg gcagcggcaa tgccgaaacc gattccgacc cgcttgccga cagcgacccg
     1561 ttctacgaat ttttcaaacg cctcgtcccg aatatgcccg aaatccccca agaagaagca
     1621 gatgacggcg gattgaactt cggttcgggc ttcatcatca gcaaagacgg ctatattctg
     1681 accaatacgc acgtcgttac cggcatgggc agtatcaaag tcctgctcaa cgacaagcgc
     1741 gaatataccg ccaaactcat cggttcggat gtccaatccg atgtcgccct tctgaaaatc
     1801 gacgcaacgg aagagctgcc cgtcgtcaaa atcggcaatc ccaaagattt gaaaccgggc
     1861 gaatgggtcg ccgccatcgg cgcgcccttc ggcttcgaca acagcgtgac cgccggcatc
     1921 gtgtccgcca aaggcagaag cctgcccaac gaaagctaca cacccttcat ccaaaccgac
     1981 gttgccatca atccgggcaa ctccggcggc ccgctgttca acctgaaagg acaggtcgtc
     2041 ggcatcaact cgcaaatata cagccgcagc ggcggattca tgggcatttc cttcgccatc
     2101 ccgattgacg ttgccatgaa tgtcgccgaa cagctgaaaa acaccggcaa agtccaacgc
     2161 ggacaactgg gcgtgattat tcaagaagta tcctacggtt tggcacaatc gttcggtttg
     2221 gacaaagccg gcggcgcact gattgccaaa atcctgcccg gcagccccgc agaacgtgcc
     2281 ggcctgcagg cgggcgacat cgtcctcagc ctcgacggcg gagaaatacg ttcttccggc
     2341 gaccttcccg ttatggtcgg cgccattacg ccgggaaaag aagtcagcct cggcgtatgg
     2401 cgcaaaggta aggaaatcac cgttgccgtc aaactgggca atgcttccga acaaaccggt
     2461 tcctcgtccg agccggacaa agccccttat gccgaacacc aatccggtac gttctcggtc
     2521 gaatccgcag gcattaccct tcagacacat accgacagca gcggcggacg gcttgtcgtc
     2581 gtgcgggttt cgggggcggc agaacgcgca ggcttgaggc gcggcgacga aatccttgcc
     2641 gtcgggcaag tccccgtcaa tgacgaagac ggtttccgca aagctatgga caaggcaggc
     2701 aaaaacgtcc ccctgctggt catgcgccgt ggcaacacgc tgttcatcgc attaaacctg
     2761 caataacaca cataatttaa ccaaaatatt ttttaaatga taaaatgccc gtcaataccg
     2821 attaacgggc attttacaga aatcagttgg ttgattttaa acaaatattt tttacgacaa
     2881 aaaatatttc cgcccatttt gccaacaaaa cccaaaccgg aggcggacag atacgctccc
     2941 atccggacgc ttaacacccc cgtaaaccat gcagctgatc gactattcac attcattttt
     3001 ctcggttgtg ccaccctttt tggcactggc acttgccgtc attacccgcc gcgtactgct
     3061 gtctttaggc atcggtattc tggtcggcgt tgcctttttg gtcggcggca accccgtcga
     3121 cggtctgaca cacctgaaag acatggtcgt cggcttggct tggtcagacg gcgattggtc
     3181 gctgggcaaa ccaaaaatct tggttttcct gatacttttg ggtattttta cttccctgct
     3241 gacctactcc ggcagcaatc aggcgtttgc cgactgggca aaacggcaca ttaaaaaccg
     3301 gcgcggcgcg aaaatgctga ccgcctgcct cgtgttcgta acctttatcg acgactattt
     3361 ccacagtctc gccgtcggtg cgattgcccg ccccgttacc gacaagttta aagtttcccg
     3421 cgccaaactc gcctacatcc tcgactccac tgccgcgcct atgtgcgtgc tgatgcccgt
     3481 ttcaagctgg ggcgcgtcga ttatcgccac gcttgccgga ctgctcgtta cctacaaaat
     3541 caccgaatac acgccgatgg ggacgtttgt cgccatgagc ctgatgaact attacgcact
     3601 gtttgccctg attatggtgt tcgtcgtcgc atggttctcc ttcgacatcg gctcgatggc
     3661 gcgtttcgaa caagccgcgt tgaacgaagc ccacgatgaa actgccgctt cagacgctac
     3721 caaaggtcgt gtttacgcac tgattattcc cgttttggcc ttaatcgcct caacggtttc
     3781 cgccatgatc tacaccggtg cacaggcaag cgaaaccttc agcattttgg gtgcatttga
     3841 aaatacggac gtgaacactt cgctggtatt cggcggcact tgcggcgtgc ttgccgtcgt
     3901 cctctgcacg ctcggcacga ttaaaaccgc cgattatccc aaagccgttt ggcagggtgc
     3961 gaaatccatg ttcggcgcaa tcgccatttt aatccttgcc tggctcatca gtacggttgt
     4021 cggcgaaatg cacacgggcg actacctctc cacgctggtt gcgggcaaca tccatcccgg
     4081 cttcctgccc gtcatccttt tcctgctcgc cagcgtgatg gcgtttgcca caggcacaag
     4141 ctgggggacg ttcggcatca tgctgccgat tgccgccgcc atggcggtca aagtcgaacc
     4201 cgcgctgatt atcccgtg
//
LOCUS       DAWHSK010000174         4149 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.174, whole genome shotgun sequence.
ACCESSION   DAWHSK010000174 DAWHSK010000000
VERSION     DAWHSK010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4149
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.174"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(163..1566)
                     /locus_tag="WIC03_001622"
     CDS             complement(163..1566)
                     /locus_tag="WIC03_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEZ3884095.1"
                     /translation="MTLLNLMIMQDYGISVCLTLTPYLQHELFSAMKSYFSKYILPVS
                     LFTLPLSLSPSVSAFTLPEAWRAAQQHSADFQASHYQRDAVRARQQQAKAAFLPHVSA
                     NASYQRQPPSISSTRETQGWSVQVGQTLFDAAKFAQYRQSRFDTQAAEQRFDAAREEL
                     LLKVAESYFNVLLSRDTVAAHAAEKEAYAQQVRQAQALFNKGAATALDIHEAKAGYDN
                     ALAQEIAVLAEKQTYENQLNDYTDLDSKQIEAIDTANLLARYLPKLERYSLDEWQRIA
                     LSNNHEYRMQQLALQSSGQALRAAQNSRYPTVSAHVGYQNNLYTSSAQNNDYHYRGKG
                     MSVGVQLNLPLYTGGELSGKIHEAEAQYGAAEAQLTATERHIKLAVRQAYTESGAARY
                     QIMAQERVLESSRLKLKSTETGQQYGIRNRLEVIRARQEVAQAEQKLAQARYKFMLAY
                     LRLVKESGLGLETVFAE"
     gene            complement(1635..3062)
                     /locus_tag="WIC03_001623"
     CDS             complement(1635..3062)
                     /locus_tag="WIC03_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HEZ3884096.1"
                     /translation="MFFSALKSFLSRYITVWRNVWAVRDQLKPPKRTAEEQAFLPAHL
                     ELTDTPVSAAPKWAARFIMAFALLALLWSWFGKIDIVAAASGKTVSGGRSKTIQPLET
                     AVVKAVHVRDGQHVKQGETLAELEAVGTDSDVVQSEQALQAAQLSKLRYEAVLAALES
                     RTVPHIDMAQARSLGLSDADVQSVQVLAQHQYQAWAAQDAQLQSALRGHQAELQSAKA
                     QEQKLVSVGAIEQQKTADYRRLRADNFISEHAFLEQQSKSVSNWNDLESTRGQMRQIQ
                     AAIAQAEQNRVLNTQNLKRDTLDALRQANEQIDQYRGQTDKAKQRQQLMTIQSPADGT
                     VQELATYTVGGVVQAAQKMMVIAPDDDKMDVEVLVLNKDIGFVEQGQDAVVKIESFPY
                     TRYGYLTGKVKSVSHDAVSHEQLGLVYTAVVSLDKHTLNIDGKAVNLTAGMNVTAEIK
                     TGKRRVLDYLLSPLQTKLDESFRER"
     gene            3298..3651
                     /locus_tag="WIC03_001624"
     CDS             3298..3651
                     /locus_tag="WIC03_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884097.1"
                     /translation="MFTTNDLRHFLEGLAILFSIGYWGTMLLLLWFLVRFAYKKPKRN
                     PGKILSGSLTAAAILMLFVWIIPKQFGPIKEEIQAQEEWDRKYKEAEAVFNEQCKTAG
                     ERFTRRRTMWKGLCC"
     gene            3940..>4149
                     /locus_tag="WIC03_001625"
     CDS             3940..>4149
                     /locus_tag="WIC03_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884098.1"
                     /translation="MAGATIRIIDRQTDEVIAKKTIYVFEKGLDGTGGARMPWKFAIL
                     CNKERLTSSEPLSDFVLSVLKPYILR"
BASE COUNT          975 a         1103 c         1019 g         1052 t
ORIGIN      
        1 tatacatttg aattaattcg cacttaattt aaatgtgttt ttaactgtgc tttatttaaa
       61 ggcaatgaga atgtgaaagt atcggatcaa tcccaaagca gcctgcactt tcgaaacggg
      121 gtgcaggctg ctttgggaat ttcataaccg tttcagcctg ctttattccg caaataccgt
      181 ttccaaccct aacccgctct ctttcaccaa gcgcaaataa gccagcatga atttataccg
      241 tgcttgagcc agtttctgtt ctgcttgggc gacttcctgc cgcgcccgta ttacttccag
      301 ccggttgcgg atgccgtatt gttggccggt ttcggtcgat ttcagtttca aacggctgct
      361 ttccaaaacc cgttcttgcg ccatgatttg gtaacgcgcc gcaccgcttt cggtataagc
      421 ctggcgtacg gcgagtttga tgtgccgctc ggttgcggtc agctgtgctt cggcggcccc
      481 gtattgcgct tcggcttcat ggattttgcc cgacaattct ccgccggtat aaagcggcaa
      541 attcaactgt acgccgacgc tcatcccttt gccccgatag tggtagtcat tattctgcgc
      601 agatgaagtg tagaggttat tctgatagcc gacatgggca gaaacggtgg gatagcggct
      661 gttctgtgct gcccgaagcg cctgtccgct gctttgcagg gcaagctgct gcatccggta
      721 ttcatgattg ttggataagg caatgcgctg ccattcatcc agactgtaac gttccagctt
      781 gggcagatag cgtgccaaca ggttggcggt atctatggcc tcgatttgtt tgctatccag
      841 gtcggtgtag tcgttcaact ggttttcata ggtttgtttc tcagccaata cggcgatttc
      901 ttgggccagg gcattgtcgt aaccggcttt ggcttcgtga atatccagcg cggtggcagc
      961 acctttattg aataaagcct gcgcctgcct tacctgctgg gcataagcct ctttttccgc
     1021 cgcatgggcg gcaacggtgt ctcggctgag taaaacgttg aaataacttt cggcaacttt
     1081 caacagcaat tcttcgcgtg ccgcatcgaa acgctgttct gcagcctgcg tatcgaacct
     1141 gctttggcgg tattgtgcaa atttggcagc gtcaaataag gtttgtccca cctgcacgct
     1201 ccatccctgt gtttcgcggg tggaagaaat cgatggcggc tggcgctggt agctggcatt
     1261 ggcggataca tggggaagga atgcggcctt ggcttgttgt tgccgtgcgc gcactgcatc
     1321 acgctggtaa tgggacgctt gaaaatcagc cgaatgttgc tgcgccgccc gccatgcttc
     1381 aggcagcgta aaagccgaaa cggatgggga aagggatagt ggcaaggtaa aaagtgaaac
     1441 gggtaggata tatttggaaa aataggattt catagccgaa aatagttcat gttgcaaata
     1501 gggcgtcagt gtcaggcaaa cggaaatacc gtaatcttgc attatcatta gattgagcaa
     1561 tgtcatccgg gcaatggttt caggcagtct gcatgtccga accggcggat aacaaatgcc
     1621 cagtacggat ccgcctatcg ctccctaaag ctttcgtcca atttggtttg cagcgggctt
     1681 aacagataat ccagcacccg ccgtttaccc gttttaatct ccgccgtgac attcatgccc
     1741 gccgtcagat tcactgcttt gccgtcaata ttcaaggtat gtttgtccag cgacaccacc
     1801 gccgtataaa ccaagcccaa ctgttcgtgg cttaccgcat catggctgac acttttcacc
     1861 ttgcccgtca gataaccgta gcgcgtatag ggaaagctct caatcttcac caccgcatcc
     1921 tgtccctgtt ccacaaaacc gatgtctttg ttcaatacca aaacttccac gtccattttg
     1981 tcgtcatcgg gcgcaatcac catcattttt tgggcagcct gcaccacacc gcccaccgta
     2041 taggtagcca attcctgcac cgtgccgtcc gcaggcgact gtattgtcat cagctgctgc
     2101 cgctgctttg ccttatccgt ttggccgcgg tattggtcaa tctgttcgtt tgcctggcgc
     2161 agcgcatcca gcgtatcgcg tttcaggttc tgcgtattca gcacccgatt ctgctccgcc
     2221 tgtgcaatgg ccgcctgaat ctgcctcatc tgaccgcgcg tactttccaa atcgttccaa
     2281 ttgctgaccg atttgctctg ctgctccaaa aacgcatgtt ccgaaataaa attgtcggcc
     2341 cgcaaacggc ggtagtctgc tgttttctgc tgctcgatcg cccccaccga aaccagcttc
     2401 tgctcctgcg ccttggccga ctgcaattcc gcctgatggc cgcgcaaagc cgactgcaat
     2461 tgcgcatcct gcgccgccca tgcctgatac tggtgctgcg ccaacacctg caccgattgc
     2521 acatcggcat cggagagacc taaagaccgt gcttgcgcca tatcgatatg cggcacggta
     2581 cggctttcca atgccgccaa taccgcttca taacgcagtt tggacaattg ggcagcctgc
     2641 aaagcctgct ccgactgcac cacatcgctg tctgttccca cagcctccag ttccgccagc
     2701 gtttctccct gtttcacatg ctgcccgtcg cgcacatgta ccgccttaac caccgccgtt
     2761 tccagcggct ggatggtttt gctgcgcccg cccgacaccg ttttgcccga agccgccgcc
     2821 acaatatcga ttttgccgaa ccaggaccac aacaaagcca aaagcgcaaa cgccataata
     2881 aaacgcgccg cccatttcgg agcggcagag accggcgtat cggtcagttc caaatgcgcg
     2941 ggcaaaaacg cctgttcttc cgccgtgcgt ttgggcggtt tcaactggtc gcgcaccgcc
     3001 caaacattgc gccatacagt aatgtatcga gaaagaaagg atttcagggc ggagaaaaac
     3061 ataacgggta taaccttggc aatatagaaa caggaaacaa tataaatatg taaaggaatt
     3121 ttaacggaaa gcgcggcagc tgttaaggga aaggcgggaa tattgacaaa aaatacccaa
     3181 gtcgttacaa atattcatta ttttactgcg taacgcaacg ctgaagcgca ggctgctttt
     3241 gagatgcggc aaggttcggc aaaaagcagc ctgcacattt aaccacagga acaacccatg
     3301 tttaccacaa acgatttacg ccatttccta gaaggtttgg ccatcctatt ctcaatcggc
     3361 tattggggca ccatgctgct gttgctttgg tttctcgtcc gctttgccta taaaaagccc
     3421 aaacggaacc ccggcaaaat cctttcaggc agcctgaccg ccgccgctat cctgatgctg
     3481 tttgtttgga ttattcccaa acaattcggc ccgatcaaag aagaaataca ggcacaagaa
     3541 gagtgggaca gaaaatacaa agaagccgaa gccgtgttta acgaacaatg caaaacggcg
     3601 ggggaaagat ttaccagacg gcggacaatg tggaagggat tatgctgttg aaggtagtac
     3661 ctgagcgtac cgtttcggca gatgcaaaaa ccagagaccc gatgtgggac aatgcggctt
     3721 tacagaccag cgaaggcgta aattttattg ctcgtttcct aggatttttt agcgatgggg
     3781 aataccgcta tgtggatgtc ctgcaaccca accattccga tattattcgg tattcaggta
     3841 aagatttttc cgctaaatca aatacttaat catatacacc ccgcccgtta tgcggtaacg
     3901 ttcgaaaaca atgtcgattc caagctgcgc aggcactggg tggcaggtgc gaccatacgg
     3961 attatcgacc gccaaactga cgaagtgatt gccaagaaaa ccatctatgt ctttgaaaaa
     4021 ggcttggacg gcacgggtgg ggcgagaatg ccgtggaagt ttgctatctt gtgcaataaa
     4081 gaaagactta cttcttcaga gccgttatcg gattttgttc ttagcgtttt aaaaccttat
     4141 atattgcgt
//
LOCUS       DAWHSK010000175         4137 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.175, whole genome shotgun sequence.
ACCESSION   DAWHSK010000175 DAWHSK010000000
VERSION     DAWHSK010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4137
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.175"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(107..1030)
                     /gene="era"
                     /locus_tag="WIC03_001626"
     CDS             complement(107..1030)
                     /gene="era"
                     /locus_tag="WIC03_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEZ3884099.1"
                     /translation="MDIETFLAEERAADGYRCGFVAIVGRPNVGKSTLMNHLIGQKIS
                     ITSKKAQTTRNRVTGIYTDDTAQFVFVDTPGFQTDHRNALNDRLNQNVTEALGGVDAV
                     VFVVEAMRFTDADRVVLKQLPKHTPVVLVVNKIDKDKAKDRYALEAFVAQVRAEFEFA
                     AAEAVSAKHGLRIANLLELIKPYLPESVPMYPEDMVTDKSARFLAMEIVREKLFRYLG
                     EELPYAMNVEVEQFEEEDGLNRIYIAVLVDKESQKAILIGKGGERLKKISTEARLDME
                     KLFDTKVFLKVWVKVKSGWADDIRFLRELGL"
     gene            complement(1077..1796)
                     /gene="rnc"
                     /locus_tag="WIC03_001627"
     CDS             complement(1077..1796)
                     /gene="rnc"
                     /locus_tag="WIC03_001627"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEZ3884100.1"
                     /translation="MKDDVLKQQAHAAIQKKLGYTFRDISLLRQALTHRSHHAKHNER
                     FEFVGDSILNYTVARMLFDAFPKLTEGELSRLRASLVNEGVLAEMAAEMNVGDGLYLG
                     AGELKSGGFRRPSILADAMEAMFAAVSFDADFNTAEKVVRHLFADRVRRADFQNQAKD
                     GKTALQEALQARRFALPKYRIEEQIGHANDSMFVISCDLGELGFVCRAKGTSRKAAEQ
                     SAAKEALQWLEEKLPLKKKKK"
     gene            1963..2286
                     /locus_tag="WIC03_001628"
     CDS             1963..2286
                     /locus_tag="WIC03_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HEZ3884101.1"
                     /translation="MNAFAQALSSALDRCIATNTVAKQNQSVGFLYREAPVFENDSGW
                     RFFSGDETDEYTDDPDNFSIVSLADIAKTNPETAALLSQPEGSAWELAEDGTFQAVAD
                     WQPQD"
     gene            2341..2817
                     /locus_tag="WIC03_001629"
     CDS             2341..2817
                     /locus_tag="WIC03_001629"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEZ3884102.1"
                     /translation="MNTIAPNLDGKHLRIGIVQARFTNEIGSEMLKVCCRTLQELGVA
                     DENITVATVPGALEIPITLMNLASSEKFDALIAIGVVIRGETYHFELVSNESGAGVSR
                     VALDYNIPIANAVLTTENDAQAIERIEEKASDAAKVAVECANLVNLLLEEQFEDEE"
     gene            2895..3320
                     /gene="nusB"
                     /locus_tag="WIC03_001630"
     CDS             2895..3320
                     /gene="nusB"
                     /locus_tag="WIC03_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEZ3884103.1"
                     /translation="MKTARRRSRELAVQAVYQSLINRTAAPEIAKNIREMSDFAKADE
                     ELFNKLFFGTQTNAAEYIRQIRPLLDRDEKDLNPIERAVLLTACHELSAMPETPYPVI
                     INEAIEVTKTFGGTDGHKFVNGILDKLAAQIRPDEPKRR"
     gene            complement(3687..>4137)
                     /gene="pyrC"
                     /locus_tag="WIC03_001631"
     CDS             complement(3687..>4137)
                     /gene="pyrC"
                     /locus_tag="WIC03_001631"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEZ3884104.1"
                     /translation="PQHLLLNRNDLLVGGVRPHHFCLPVLKRETHRQALVAAVTGEKA
                     HKFFLGTDSAPHAKSAKENACGCAGMFSAMTAIELYAEVFEKAGALDKLEAFTSKNGA
                     RFYGIPENAGTITLVKQSQTVPASVPYGDGELVPMRAGGEIGWTVQY"
BASE COUNT         1004 a         1272 c          944 g          917 t
ORIGIN      
        1 tgcacagata atatcaaccc gtttttaaca aagatattcc cgattgaaac aaaatgccgt
       61 ctgaaacctg ttcggacggc atttgcgtaa agttcagcaa gaaaaactac aaacccagct
      121 cgcgcaggaa gcggatgtcg tccgcccaac cggatttgac tttgacccac actttcaaaa
      181 acactttggt atcaaacagt ttttccatat ccaaccgcgc ttcggtggaa attttcttca
      241 aacgctcccc gcctttgccg attaaaatcg ccttttggct ttccttatcg accaaaacgg
      301 cgatatagat gcggtttaaa ccgtcttcct cttcaaactg ctccacttcg acgttcatcg
      361 cataaggcaa ttcctcgccc aaatagcgga acaatttttc acgcacgatt tccatcgcta
      421 aaaaacgcgc cgatttgtcc gtaaccatgt cttcgggata catcggcacg ctttcgggca
      481 gatacggttt aatcagctcc aacaggttgg caatccgcaa tccgtgtttc gcgctgactg
      541 cctccgctgc cgcaaattca aattcggcgc gcacctgggc aacaaacgcc tccaacgcgt
      601 aacggtcttt cgccttgtcc ttgtcaattt tgttgaccac taaaacgacc ggcgtgtgct
      661 tgggcagttg tttcaacacg actcggtcgg catcggtaaa gcgcatcgcc tccacgacga
      721 aaaccacggc atccacaccg ccgagtgcct cggtaacgtt ttggttcaaa cggtcgttga
      781 gcgcgttgcg gtggtcggtt tgaaaaccgg gcgtatcgac aaacacgaac tgcgcggtat
      841 cgtcggtata aatccccgta acacggttgc gcgtcgtctg cgcctttttg ctggtgatgc
      901 tgattttctg accgatgaga tggttcatca gcgttgattt gcccacgttc ggacggccga
      961 caatcgctac gaagccgcaa cggtaaccgt cggcggcacg ttcctctgca aggaaggttt
     1021 caatatccat attcgctttc atgtccacat ttcagacggc atattcacac gccgcctcat
     1081 ttctttttct tcttcagcgg cagcttttct tccagccact gcagcgcctc tttcgccgcc
     1141 gactgctccg ccgccttacg gctcgtccct ttggcacggc acacgaaacc cagttcgccc
     1201 aaatcgcagg aaatcacaaa catactgtcg ttggcatgac cgatttgctc ttcgatacgg
     1261 tatttcggca aggcgaaacg gcgcgcctgc aacgcctcct gcaaagcagt tttgccgtct
     1321 tttgcctgat tttggaaatc ggcacgccgg acgcgatcgg caaacaaatg gcgcaccacc
     1381 ttttccgccg tgttgaaatc ggcatcgaag ctgacggcgg caaacatcgc ctccatcgcg
     1441 tccgccagta tcgaaggccg tctgaagccg ccgctcttca actcgcccgc ccccaagtac
     1501 agaccgtcgc cgacattcat ttccgccgcc atttccgcca gcacgccctc attgaccaga
     1561 cttgcccgca accgcgacaa ctcgccctcg gtcaacttcg gaaacgcgtc aaacagcatc
     1621 cgcgccaccg tataattcaa aatcgaatca ccgacaaact cgaaccgctc gttgtgcttc
     1681 gcatgatggc tcctgtgcgt caaagcctgc cgcaaaagcg aaatatcgcg gaacgtgtag
     1741 cccagtttct tctgtatcgc cgcgtgtgcc tgctgtttca aaacatcgtc tttcattacc
     1801 gtcttccgca aaaaacagcc cgaatgcgtt tccatactcc aaaaatacag aaacacctta
     1861 aggctgcaaa cggcgcacat tgtaccacca aaccgccgcc gtctgccgac aacgcacgaa
     1921 tcgtggtaaa atccgtaagt tttcccccaa cgtattcccg taatgaacgc atttgcacaa
     1981 gcactttcct cggcactcga ccgctgtatc gccaccaaca ccgtcgccaa acaaaaccag
     2041 tctgtcggct tcctctaccg cgaagccccc gttttcgaaa atgacagcgg ctggcgtttc
     2101 ttcagcggcg acgagaccga cgaatacacc gacgatccgg acaacttcag catcgtcagc
     2161 ctcgccgaca tcgccaaaac caaccctgaa accgccgcac tgctttccca acccgaaggc
     2221 agcgcgtggg agcttgccga agacggcaca ttccaagccg ttgccgactg gcagccgcaa
     2281 gactgaaacc ttcagacggc atcccaacca tccaaattac cgatccacaa aggaatccag
     2341 atgaacacca tcgcccccaa cctcgacggc aaacacctcc gcatcggcat cgtacaggca
     2401 cgtttcacca acgaaatcgg cagcgaaatg ctcaaagtct gctgccgcac cctccaagaa
     2461 ttgggcgtgg cagacgaaaa cattaccgtc gccaccgtac ccggcgcact tgaaatcccc
     2521 atcacgctga tgaaccttgc ctcttctgaa aaatttgacg cactgattgc catcggcgtc
     2581 gttatccgtg gcgaaaccta ccatttcgag ctggtttcca acgaatccgg agcaggggtc
     2641 agccgcgtcg cactcgacta caacatcccg attgccaatg ccgtcctgac caccgaaaac
     2701 gacgcacagg caatcgaacg gattgaagaa aaagcctcgg atgccgccaa agtcgccgta
     2761 gaatgcgcca acctcgtcaa ccttctgctc gaagaacagt ttgaagacga agaataacaa
     2821 acagggcgta caccgatgcg ccctttccgt ttcagacggc ataccgtgcc gcccaaccgt
     2881 ttcaaggaaa aattatgaaa acagcccgcc gccgttcccg cgagcttgcc gtacaagccg
     2941 tttaccaatc ccttatcaac cgcaccgcag cacccgagat tgctaaaaac atccgcgaaa
     3001 tgtccgactt tgccaaagcg gacgaagaat tgttcaacaa acttttcttc ggcacgcaaa
     3061 ccaatgcggc agaatacatc cggcaaatcc gcccgctgct cgacagggac gaaaaagacc
     3121 tcaaccccat cgaacgcgcc gtcctgctga ccgcctgcca cgagttgtcc gccatgcccg
     3181 aaacgcccta ccccgtcatc atcaacgaag ccatcgaagt caccaaaacc ttcggcggta
     3241 cggacgggca caaattcgtc aacggcatcc tcgacaaact cgccgcccaa atccgccccg
     3301 acgagcccaa acgccgttga tgcatcacaa atttaaagaa agttctatgt ccttatttct
     3361 gctttctttg tttcgtcatt cccacgcagg tgggaatttg gttttttgaa ttttgggtat
     3421 ttcaaataaa ttctgaaact tcaatctcgt cattcccgcg caggcgggaa tccggttttt
     3481 tgagttgctg ccgtttttga taaactctgg tcgcatcgaa tttccggatt cctgccttcg
     3541 tgggaatggc gaaatttgaa aattgtggga atttatcgga aataaccgaa acgtaacaaa
     3601 ctcaaacgcc gttgacgtat caatcagtca acaacacaaa tgccgtctga aaaacctttc
     3661 agacggcatt ttgcttgacc gttcaatcaa tactgtaccg tccagccgat ttcgccgccc
     3721 gcgcgcatcg ggacaagttc gccgtcgccg tagggaacac ttgcggggac ggtttggctt
     3781 tgtttgacga gggtgatcgt gccggcgttt tccggaatgc cgtaaaacct tgcgccgttt
     3841 ttcgaggtaa aggcttcgag tttgtccaac gcgcctgctt tttcaaacac ttcggcgtaa
     3901 agctcgatgg cggtcatcgc gctgaacata ccggcgcaac cgcaggcgtt ttctttagcg
     3961 gatttggcgt gcggcgcgga atctgtgccg aggaagaatt tgtgcgcctt ctcgccggta
     4021 acggcggcaa ccaatgcctg acggtgggtt tcgcgtttga gtacgggcag gcagaaatga
     4081 tgggggcgca cgccgccgac caagaggtcg ttgcggttga gcaagaggtg ttgcggg
//
LOCUS       DAWHSK010000176         4128 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.176, whole genome shotgun sequence.
ACCESSION   DAWHSK010000176 DAWHSK010000000
VERSION     DAWHSK010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4128
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.176"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            315..1109
                     /locus_tag="WIC03_001632"
     CDS             315..1109
                     /locus_tag="WIC03_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEZ3884105.1"
                     /translation="MTTILAFDIETVPDVQGIRTLYELPSSLPDDEVVLFAQQKRRAQ
                     TGGDFMQHHLHQVVAISCCMRWGQDKVHVGTIGEMDDGEEVVIAKFFELIEKHTPQLV
                     SWNGGGFDLPVLHYRSLLHGINAARYWDMGDGDFGDSRDFKWNNYISRYHQRHCDLMD
                     LLALYQPRANVPLDDMAKLCGFPGKLGMDGSKVWDAFHAGRLKEIRNYCETDAVNTYL
                     MYLRFCLVSGRFDADEYEMEIKRIRNYLSAQTEDKPHWAEFVQAWK"
     gene            1320..3923
                     /gene="pepN"
                     /locus_tag="WIC03_001633"
     CDS             1320..3923
                     /gene="pepN"
                     /locus_tag="WIC03_001633"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HEZ3884106.1"
                     /translation="MSKTVRYLKDYQTPAYRILETDLHFDIAEPQTVVKSRLTVEPQR
                     VGEPLVLDGSAKLLSVKINGAAADYMLEGETLTIADVPSERFTVEVETEILPAENKSL
                     MGLYASAGNLFTQCEPEGFRKITFYIDRPDVMSKFTTTIVADKKRYPVLLSNGNKIDG
                     GEYSDGRHWVKWEDPFAKPSYLFALVAGDLAVTEDYFTTMSGRNVKIEFYTAEADKPK
                     VGFAVESLKNAMKWDETRFGLEYDLDIFMVVAVGDFNMGAMENKGLNIFNTKFVLADS
                     RTATDTDFEGIESVVGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSGDRASRA
                     VRRIENIRLLRQHQFPEDAGPTAHPVRPASYEEMNNFYTMTVYEKGAEVVRMYHTLLG
                     EEGFQKGMKLYFQRHDGQAVTCDDFRAAMADANGINLDQFALWYSQAGTPVLEAEGRL
                     KNNSFELTIKQTVPPTPDMADKQPMMIPVKIGLLNRNGEAAAFDYQGKRATEAVLLLT
                     EAEQIFLLEGVTEAVVPSLLRGFSAPVHLNYPYSDDDLLLLLAHDSDAFTRWEAAQTL
                     YRRAVAANLAALSDGIGLPKHEKLLAAVEKVISDDLLDNAFKALLLGVPSEAELWDGA
                     ENIDPLRYHQAREALLDILAVRFLPKWHELNRQAAKQENQSYEYSPEAAGWRTLRNVC
                     RAFVLRADPAHIETVAEKYAEMAQNMTHEWGILSAVNGNESDTRNRLLAQFADKFSDD
                     ALVMDKYFALVGSSRRSDTLQQVQTALQHPKFSLENPNKARSLIGSFSRNVPHFHAED
                     GSGYRFIADKVIEIDRFNPQVAARLVQAFNLCNKLEPHRKNLVKQALQRIRAQEGLSK
                     DVGEIVGKILD"
BASE COUNT         1004 a         1120 c         1130 g          874 t
ORIGIN      
        1 atgcttggca aagcgtagaa aatggcgtac atttcgctac atggaattac acgacaggca
       61 ggaaatgctg tttgaaagga ttttccggtc agtcttgcga ttggtcgggg tttcatcgga
      121 aacggtgaaa caaaagtttg ccggcgtaga gttgagctac gcgggtaaag ccgcaggcga
      181 aagcctgtat tgtttgtgaa gcgtaaatct ctgatttgag gtattggggc aatcctgtgg
      241 ggggttgcct ctttttttat cggcctttta atgacacaat aggcgcaagt ctgttttaaa
      301 tacaaggtgc tgtcatgacc acgattttgg ctttcgatat tgaaaccgta cctgatgtcc
      361 agggtatccg aacattgtac gagctgccgt cctcgctgcc cgacgatgaa gtggtgctgt
      421 ttgcccagca aaaacgccgt gctcagacgg gcggcgattt tatgcagcat catcttcatc
      481 aggttgtggc gatttcgtgc tgcatgcgtt gggggcagga taaagtacat gtcggcacca
      541 tcggcgagat ggatgacggc gaggaagtgg tcattgctaa atttttcgaa ttgattgaaa
      601 aacatacgcc gcaactggtc agttggaacg gcggcggttt cgacctgccg gttctgcatt
      661 accgttcgct gctacacggc atcaacgccg cgcgctattg ggatatgggc gacggcgatt
      721 tcggcgacag ccgcgatttc aagtggaaca actacatcag ccgttaccac caacgccact
      781 gcgatttgat ggatttgctc gcactttatc agccgagggc aaacgtgccg ctggacgata
      841 tggcgaaact gtgcggtttt ccgggcaagc tgggtatgga cggcagcaag gtgtgggatg
      901 cgttccacgc gggcaggctg aaagaaatcc gcaattattg cgaaaccgat gccgtgaata
      961 cgtatttgat gtatctgcgt ttttgtctgg tcagcggcag attcgacgcg gacgaatacg
     1021 aaatggaaat caagcggatc agaaactatc tttccgccca aacagaagac aaaccgcatt
     1081 gggcagaatt tgtccaagcg tggaaataga aaatctgctg tccgaaggcg gtacgcttgt
     1141 gccgccgttt cggttcagac ggcatatggt ggattaaatt caaaccggta cagcccgtac
     1201 tatcagtact gtctgcggct tcgtcgcctt gtcctgattt ttgttaatcc gctttaaaag
     1261 ctgtatccgt gccgtctgaa gccttttcca gtcaaaccaa acaatattga aagaaaaaaa
     1321 tgagcaaaac cgtccgttat ctgaaagatt accaaacgcc tgcctaccgc attctcgaaa
     1381 ccgacctgca tttcgatatt gccgaaccgc aaaccgtcgt gaagtcgcgt ttgacggttg
     1441 agccgcagag ggtaggggag ccgctggtgt tggacggttc ggcgaaactc ttgtccgtca
     1501 aaatcaacgg cgcggcggcg gattatatgt tggaaggcga gacgctgacg attgcagacg
     1561 tgccgtccga acgcttcacc gtcgaagtgg aaaccgaaat cctgccggcg gaaaacaaat
     1621 cgctgatggg gctgtatgcg tccgccggta acctgtttac ccagtgcgag ccggagggct
     1681 tccgcaaaat cacgttctat atcgaccgtc cggatgtcat gtccaagttc acgaccacca
     1741 tcgtcgcgga caaaaaacgc tatcccgttt tgctctccaa cggcaacaaa atcgacggcg
     1801 gcgagtattc agacggccgc cattgggtga aatgggaaga cccgtttgcc aaaccgagtt
     1861 atctgtttgc tttggtcgcg ggcgatttgg cggtcacgga agactatttc accaccatga
     1921 gcggcagaaa cgtcaaaatc gagttttaca ctgccgaggc ggacaagcct aaggtcggct
     1981 ttgccgtgga atccttgaaa aacgcgatga agtgggacga aacgcgcttc ggtttggaat
     2041 acgacttgga tattttcatg gtcgtcgccg tgggcgattt caatatgggt gcgatggaaa
     2101 acaaggggct gaacattttt aataccaagt tcgtccttgc cgacagccgt accgccaccg
     2161 ataccgattt tgaaggcatc gaatccgtgg tcggacacga gtatttccac aactggacag
     2221 gcaaccgcgt tacctgccgc gattggttcc agctttcgct gaaagaaggg ttgaccgtgt
     2281 tccgcgacca agaattttcc ggcgaccgcg ccagccgcgc cgtgcgccgc atcgagaaca
     2341 tccgcctgct gcgccagcac cagttccccg aagacgcagg cccgaccgcc catccggtgc
     2401 gccccgccag ctatgaggag atgaacaatt tctacaccat gaccgtttat gaaaaaggcg
     2461 cggaagtggt gcggatgtat cacaccttgc tcggcgaaga gggcttccaa aaaggcatga
     2521 agctctattt ccaacgacac gacgggcagg ccgtgacctg cgacgatttc cgcgcggcga
     2581 tggcggacgc gaacggcatc aatctcgacc agttcgcctt gtggtacagc caggcgggta
     2641 cgcccgtctt ggaagcggaa ggtcgtctga aaaacaatag tttcgagtta accatcaaac
     2701 aaaccgtgcc gcccacgccc gatatggcgg acaagcagcc gatgatgatt cccgtcaaaa
     2761 tcgggctgct gaaccgcaac ggcgaagcgg cggcattcga ttatcagggc aaacgcgcga
     2821 ccgaagccgt attgctgctg accgaagccg aacagatctt cctgctcgaa ggcgtaaccg
     2881 aagccgtcgt tccctcgctg ctgcgcggct tcagcgcgcc ggtgcatctg aactatccgt
     2941 acagcgacga cgacctgctg cttctgctcg cccatgacag cgacgccttc acgcgctggg
     3001 aagccgccca aacgctctac cgccgtgccg tcgccgccaa ccttgccgcg ctttcagacg
     3061 gcatcgggtt gccgaaacac gaaaaactgc ttgccgccgt cgaaaaagtc atttcagacg
     3121 acctcttaga caacgctttc aaagccctgc ttttgggtgt gccgtctgaa gccgagctgt
     3181 gggacggcgc ggaaaacatc gacccgctgc gctaccatca ggcgcgcgaa gccttgttgg
     3241 atatacttgc cgtccgcttt ctgccgaaat ggcacgaatt gaaccgtcag gcggcgaagc
     3301 aggaaaacca aagctacgag tacagccccg aagccgccgg ttggcgcacg ctgcgcaatg
     3361 tctgccgcgc cttcgtcctg cgcgccgatc ccgcgcacat cgaaaccgtt gccgagaaat
     3421 acgccgaaat ggcgcaaaac atgacccacg aatggggcat cctgtccgcc gtcaacggca
     3481 acgaaagcga tacgcgcaac cgcctgctgg cgcagtttgc cgacaagttt tcagacgacg
     3541 cgctggtgat ggacaaatat ttcgccctcg tcggctcaag ccgccgcagc gacaccctgc
     3601 aacaggttca aaccgccttg cagcatccga agttcagcct cgaaaatccc aacaaagccc
     3661 gctcgctcat cggcagcttc agccgcaacg tcccgcattt ccacgcagaa gacggcagcg
     3721 gctaccgctt catcgccgac aaagtcatcg aaatcgaccg ctttaacccg caggtcgccg
     3781 cccgcctggt gcaggcgttc aacctctgca acaagctcga gccgcaccgc aaaaacttgg
     3841 tgaaacaagc attgcagcgc attcgggcgc aggaaggatt gtcgaaagac gtgggcgaaa
     3901 tcgtcggcaa aattttggat tgaggccgtc aaacagaaaa acaatgccgt ctgatatagt
     3961 tacacacacg ttttatcatc acttccccat cgttttatta cgcaatggca aaacggccgc
     4021 aaagtcatat taattatagt ggattaacaa aaatcaggac aaggcgacaa agccgcagac
     4081 agtacagata gtacggtaag gcgaggcaac gctgtactgg tttttgtg
//
LOCUS       DAWHSK010000177         4124 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.177, whole genome shotgun sequence.
ACCESSION   DAWHSK010000177 DAWHSK010000000
VERSION     DAWHSK010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4124
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.177"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..1023)
                     /gene="mpl"
                     /locus_tag="WIC03_001634"
     CDS             complement(<1..1023)
                     /gene="mpl"
                     /locus_tag="WIC03_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HEZ3884107.1"
                     /translation="MKHIHIIGIGGTFMGGIAAIAKEAGFEVSGCDAKMYPPMSTQLE
                     ALGIGVHEGFDAAQLEEFKADVYVIGNVAKRGMDVVEAILNRGLPYISGPQWLAENVL
                     HHHWVLGVAGTHGKTTTASMLAWVLEYAGLAPGFLIGGVPENFSVSARLPQTPRQDPN
                     SQSPFFVIEADEYDTAFFDKRSKFVHYRPRTVVLNNLEFDHADIFADLGAIQTQFHHL
                     VRTVPSEGLIICNGQQQSLQDTLDKGCWTPVEKFGTEHGWQAGEANADGSFDVLLDGK
                     TAGRVKWDLMGRHNRMNALAVIAAARHVGVGIQTACEALGAFKNVKRRMEIKGTANGI
                     TVYDDFA"
     gene            complement(1085..1390)
                     /locus_tag="WIC03_001635"
     CDS             complement(1085..1390)
                     /locus_tag="WIC03_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884108.1"
                     /translation="MRVSKIIGSMLLVTAVQTVFSANVYACRHNGKTSYSQTPGKHCT
                     NAGLGRDRVYSSVRPAVKDRAEDAGVGDYSDTVRDEHVQNPKGNAQKDGSDAGIRPH"
     gene            complement(1402..1755)
                     /locus_tag="WIC03_001636"
     CDS             complement(1402..1755)
                     /locus_tag="WIC03_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884109.1"
                     /translation="MKNCEFFYDPTRAIYDSGADYLTREKHRLVVIANSAWGLLLNLS
                     CYYDEVLEKRKIPFGKQEIDDDMDKVSALKRKFKDISEIKVGDGWEYPFNYEQGMKEL
                     DEVLLKYIPFFEEER"
     gene            complement(2017..2214)
                     /locus_tag="WIC03_001637"
     CDS             complement(2017..2214)
                     /locus_tag="WIC03_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884110.1"
                     /translation="MPSEKIYYGVLIFLCIASMLLSPFFYAGALKPKKAALRKDGQWK
                     LILLSNTVAAAVLAWVWWKWF"
     gene            complement(2224..3009)
                     /locus_tag="WIC03_001638"
     CDS             complement(2224..3009)
                     /locus_tag="WIC03_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEZ3884111.1"
                     /translation="MKHISSTNNEHIRHLHRLLSQGKFRRQYAQTVLEGVHLLQVFLQ
                     SGGKPVGVYIPEGKMPSEEIRRLRAVVPEGRIFSVSDGILKKISSLTCADDVLALIDI
                     PDAGALPAGGDCVVLDGVQDPGNVGTVLRSAAAAGIGVVVLGKGCADAWSPKVLRAGM
                     GAHFLLDIYSQADLEIWLASYKGRVFATALREEKQVVLYGEDLCEPTAWVFGNEGAGV
                     GKAVLDRADKCVRIPMHDATESLNVAMAATICLFEQMRQRSAY"
     gene            complement(3083..>4124)
                     /gene="tilS"
                     /locus_tag="WIC03_001639"
     CDS             complement(3083..>4124)
                     /gene="tilS"
                     /locus_tag="WIC03_001639"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEZ3884112.1"
                     /translation="EKNGLGIEAAARQKRYAAFAEKGFDVLALAHHRDDQIETFMLAV
                     ARGGGLRALAAMPAVRPFGEKGIIWRPLLPFSRQDIWDYAQKHGLPNIEDESNTDTAY
                     LRNRFRHRILPELSAQIPHFGRHVLNNVRALQEDLALLDEVVVQDCRWICGAGYFDTA
                     RWLTFSPRRKTHILRHFLKENGIPVPNQNALADIARVLTEAKTGCWNLQGFELHHYAG
                     RLFVFRLEKTDKLRFLKDRQISGNLREILTGQGFVLKRHPFGLPEHLLEQDGILRTVA
                     ASDTLAVGGIHKDVKKILQGKRVLPVLRPIWPLVADSGNRPLALANCCADFQYSVSDG
                     ILPVHPDFPILF"
BASE COUNT          990 a         1214 c          918 g         1002 t
ORIGIN      
        1 ggcgaagtcg tcgtaaacgg tgatgccgtt tgccgtgcct ttgatttcca tgcggcgttt
       61 gacgttttta aacgcgccca aggcttcgca ggccgtctga atgccgacac cgacatgacg
      121 cgcggcggca atgacggcga gcgcattcat gcggttgtgc ctgcccatca aatcccattt
      181 gacgcgtccg gccgttttgc cgtctagtaa cacgtcgaac gagccgtcgg cattggcttc
      241 gccggcctgc cagccgtgtt ccgtgccgaa tttctccacc ggcgtccagc agcctttgtc
      301 taaagtatcc tgcaagcttt gctgctgtcc gttacagatg attaagcctt cagacggcac
      361 ggtgcgcacg aggtggtgga actgggtctg tatcgcgccc aagtcggcaa agatgtcggc
      421 gtggtcgaat tccagattgt tcaacacgac ggtacgcggg cggtaatgta cgaatttgga
      481 gcgtttgtcg aaaaaggcgg tgtcgtattc gtcggcttcg ataacgaaaa acggagattg
      541 gctgttcggg tcttgacgcg gcgtttgcgg caggcgggcg gaaacgctaa agttttccgg
      601 tacgccgccg ataaggaagc ccggcgcgag tccggcatat tccaaaaccc atgcgagcat
      661 ggacgcggtg gtcgttttgc cgtgcgtccc cgccacaccg agtacccaat gatggtgcag
      721 cacgttttca gccagccatt gcggaccgga aatataaggc agcccgcggt tcaaaatcgc
      781 ctcaaccaca tccatcccgc gcttggcgac attgccgatg acgtaaacgt cggctttaaa
      841 ttcctccaac tgcgccgcgt cgaagccttc gtgtacgcct atacccaagg cttcgagctg
      901 ggtgctcatc ggcggataca tcttcgcgtc gcaaccgctg acttcaaacc cggcttcttt
      961 ggcaatggcg gcaatcccgc ccataaacgt gccgccgata ccgataatgt ggatgtgttt
     1021 catcagagtt ccctaatcgt tcaaattcta ttattcaacg ccgtcatggc tgattcggtt
     1081 tcaatcaatg cggcctgatg ccggcatccg aaccgtcttt ctgtgcattc cctttcggat
     1141 tttggacgtg ttcgtccctc accgtgtccg aataatcgcc gacccctgcg tcttccgccc
     1201 tgtcttttac tgccggtcta accgaactgt acacccggtc ccgccccaaa cccgcgttgg
     1261 tacaatgttt tcccggagtt tggctgtaac tggttttacc attatggcgg cacgcgtaaa
     1321 catttgccga aaatacggtc tgaaccgctg taacaagcaa catacttcca attattttag
     1381 atacgcgcat atcaacctcc tttatcgttc ttcttcaaaa aagggaatgt atttcaatag
     1441 cacttcatct aattctttca ttccctgctc ataattgaac gggtattccc aaccatcccc
     1501 tactttgatt tcagaaatat ctttaaactt ccgcttaagg gcggacactt tgtccatatc
     1561 gtcatcaatt tcctgtttgc cgaacggtat tttccgcttt tccaaaacct cgtcataata
     1621 acaagataaa ttaagtaata gcccccaagc actatttgca attacgacta atctatgttt
     1681 ttccctagtt aagtaatctg caccgctatc atagattgcc cttgtcgggt cataaaaaaa
     1741 ttcgcaattt ttcatagcgt ccccacactt taaccgattc gcaattttat atccgcgatt
     1801 ttgcaagctg cccataaaaa ccgattgcct gccaagacgg cactacttgt taaagatgcc
     1861 cgataatgcc gcaactcttc cgtatttacc gacaaacgcc ctatttgctt agagctgttt
     1921 tgttttccac aaaactttaa ttcgttgatt taacaaattt tttaccaaat tctttatttg
     1981 ttcggattcc attagcacga ttttgagcaa tatctgtcaa aaccatttcc accacaccca
     2041 agccaaaacc gccgccgcca cggtattgga caataagatg agtttccact gcccgtcctt
     2101 ccgcaatgcc gccttcttgg gcttcaaagc acccgcataa aaaaacggcg acagcagcat
     2161 agaagcgata cataagaaaa tcaatacgcc gtaatagatt ttttcagacg gcatttcttt
     2221 tcctcaatac gccgaccgct gccgcatctg ttcaaacagg cagattgtcg ccgccatcgc
     2281 gacatttaaa gactcggttg catcgtgcat cggaatcctg acacacttgt ccgccctgtc
     2341 caaaaccgct ttaccgaccc ccgcgccttc gttgccaaac acccaggctg tcggttcgca
     2401 caaatcttcg ccgtacaaaa ccacctgctt ttcctcgcgc aaggcggtgg caaacacacg
     2461 gcctttatag ctcgccaacc atatttccaa atccgcctgc gaataaatgt ccaacaagaa
     2521 atgcgcgccc atacccgcgc gtaatacctt gggcgaccac gcgtccgcgc aacctttgcc
     2581 caaaacgacc acgccgattc ccgccgccgc cgcgctccgc aacaccgtgc cgacattgcc
     2641 cgggtcttgc acgccgtcca aaaccacgca gtcgccgccg gcaggcaaag cacccgcatc
     2701 cggaatatca atcagcgcaa gcacatcatc cgcacaagtc aagctgctga tttttttcaa
     2761 tatgccgtct gaaacggaaa aaatcctgcc ttccggcaca accgccctca gcctgcggat
     2821 ttcttcagac ggcattttgc cctcgggaat atataccccg accggctttc cgccggattg
     2881 caggaaaacc tgaagcaggt gcacgccctc caaaacggtt tgggcgtatt gccgtctgaa
     2941 ctttccttgc gacaacaggc ggtgcaggtg tctgatgtgt tcattattgg tcgaactgat
     3001 gtgtttcact tcacaagcct tttagaaata tgcaaccgga caatacccga ctgaacgccg
     3061 ccaaaatctg tttgcgatat tatcaaaata aaatgggaaa gtcaggatgg acgggcaaaa
     3121 tgccgtctga aaccgagtat tggaaatccg cacaacagtt tgccaacgcc aatggacggt
     3181 ttccgctgtc ggcaacaagc ggccaaattg ggcgcaggac aggcaaaacc cgtttcccct
     3241 gaaggatttt tttcacatcc ttatggatgc cgccgacagc caacgtatcc gatgccgcta
     3301 ccgtcctcaa aattccgtcc tgctccaaaa gatgctcagg aagcccaaac ggatgccgtt
     3361 tcaacacaaa tccctgcccc gtcaatattt cccttaaatt tccgcttatc tgcctgtctt
     3421 tcaaaaaccg cagtttatcc gttttttcca gtcggaacac aaacagcctg cctgcataat
     3481 gatgcaattc aaagccttgc aagttccaac atccggtttt tgcctccgtc aaaacccggg
     3541 caatgtcggc aagggcattc tgattcggca cgggaatgcc gttttccttc agaaaatgcc
     3601 gcaaaatatg ggttttccgg cgcggggaaa acgtcagcca ccgcgccgta tcgaaataac
     3661 cggccccgca aatccaacgg cagtcctgaa cgacgacctc gtccaacaaa gccaaatctt
     3721 cctgcaaagc gcggacattg ttcagcacat gccgcccgaa atggggaatc tgcgccgaaa
     3781 gttcgggcaa aatacggtgc cggaagcggt ttcgcaaata agccgtatcg gtattgcttt
     3841 catcctcgat attcggcaaa ccgtgttttt gggcataatc ccatatatct tggcgcgaaa
     3901 aaggcagcaa gggccgccag atgatgcctt tttccccaaa agggcggacg gcgggcatag
     3961 ccgccaaagc gcgcaaaccg ccgccgcgcg cgaccgccag cataaaagtt tcgatttgat
     4021 cgtccctgtg gtgcgccaac gccaaaacgt caaagccttt ttcggcaaac gcggcataac
     4081 gcttttgcct tgccgccgcc tcgatgccca aaccgttttt ttcc
//
LOCUS       DAWHSK010000178         4117 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.178, whole genome shotgun sequence.
ACCESSION   DAWHSK010000178 DAWHSK010000000
VERSION     DAWHSK010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4117
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.178"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(245..1108)
                     /gene="rpoH"
                     /locus_tag="WIC03_001640"
     CDS             complement(245..1108)
                     /gene="rpoH"
                     /locus_tag="WIC03_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HEZ3884113.1"
                     /translation="MNNAFALPAIQSGNGSLEQYIHTVNSIPMLSQEEETRLAERRIK
                     GDLNAAKQLILSHLRVVVSIARGYDGYGLNQADLIQEGNIGLMKAVKRYEPGRGARLF
                     SFAVHWIKAEIHEFILRNWRLVRVATTKPQRKLFFNLRSMRKNLNALSPKEAQDIADD
                     LGVKLSEVLEMEQRMTGHDIAIMADNSDDEDSFAPIDWLADHDSEPSRQLSKQAHYAL
                     QTEGLQNALAQLDDRSRRIVESRWLQDDGGLTLHQLAAEYGVSAERIRQIEAKAMQKL
                     RGFLTEEAEAV"
     gene            complement(1372..2910)
                     /gene="lnt"
                     /locus_tag="WIC03_001641"
     CDS             complement(1372..2910)
                     /gene="lnt"
                     /locus_tag="WIC03_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEZ3884114.1"
                     /translation="MVSKLDKYWQHPALYWPLLILFAAATPFTFAPYYHFWLMPLIFG
                     AFVRLIELRPRFAVSSAYLFGLTAYTTQFYWIHTALHDVSGLPDLYAVPLTFLLPAYL
                     ALYPALCFWLWKKFTLPRGIKIGLVLPILWTLTEFARERFLTGFGWGAIGYSQITPDS
                     PLAGFAPLGGIHMVTLATAFLGVWLVLASDNTARSGKRLLPIILIAALLAAGYTARQT
                     DFTRPDGSRSTVALLQGNIDQTLKWREDQVIPTIQKYYEQVGKTTADIVILPETAIPV
                     MRQNLPENILAKFAEQAQNNGSALAVGISQYTSDGNGYENAVINLTGYQENNQDGIPY
                     YAKNHLVPFGEYKPLPFLTTPLYKMMNMPLSDFRKGGGKQSALLMKNQKIAFNICYED
                     GFGDELIAAAKDATLLANASNMAWYGKSNAMYQHLQQSQARAMELGRYMVRATNTGAT
                     AIISPKGNIIAQAQPDTETVLEGHIKGYVGETPYMKTGSSWWLMGILTLAALILFIFR
                     NKEH"
     gene            complement(2979..3347)
                     /locus_tag="WIC03_001642"
     CDS             complement(2979..3347)
                     /locus_tag="WIC03_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
                     /protein_id="HEZ3884115.1"
                     /translation="MAAIGIGMTVLVYGIVAVIVKLDDLGMLLMRRPQTFSRSLGQML
                     TAFMPCFMRGLSVVGTLAMFLIGGVLVAHNLGLLHDFLHAQHWDAGWAEYFANLVVGL
                     LSGSIACAPALPLMNRFGRH"
     gene            complement(3247..3705)
                     /locus_tag="WIC03_001643"
                     /pseudo
     CDS             complement(3247..3705)
                     /locus_tag="WIC03_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237797.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
     gene            3899..>4117
                     /locus_tag="WIC03_001644"
     CDS             3899..>4117
                     /locus_tag="WIC03_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium:proton antiporter"
                     /protein_id="HEZ3884116.1"
                     /translation="MDWMGSLFLPGGALLFLSVVSTTLSACLGMPLLLVSPAGVLDGA
                     AEALAIAAFLMLVARPPAVFGGLWKFNYS"
BASE COUNT          886 a          948 c         1275 g         1008 t
ORIGIN      
        1 atttgtactg tctgcggctt cgccgccttg tcctgatttt tgttaatcca ctatatcaat
       61 tccgccaatc tgtcggaaaa gcagctgatg cggcagtgtc tggtgcatgt ctgctttttg
      121 atttcggcaa ttgcaacggc gtggacggat aaaatcgtgt acagcacgac gcacaaaccg
      181 cattgatgtt taccaaataa aatacccgac aaaacaattt gtcgggtatt ttattgcgta
      241 tatttcaaac cgcttcggct tcttcggtca ggaaaccacg cagtttctgc atggcttttg
      301 cttcaatctg gcggatgcgc tcggcagaga cgccgtattc tgcagcaagc tggtgcagcg
      361 tcagcccgcc gtcgtcttga agccagcggc tttccacaat acggcggctc ctgtcatcca
      421 gttgcgccaa agcgttttgt aaaccttctg tttgcagggc gtaatgcgcc tgtttcgata
      481 gttgccggct cggttcggaa tcgtggtcgg caagccagtc gatgggggcg aaactgtcct
      541 cgtcgtcgct gttgtctgcc atgatggcga tgtcgtgtcc cgtcatgcgc tgttccattt
      601 ccagaacttc ggaaagtttg acacccaaat cgtcggcgat gtcttgtgcc tctttgggag
      661 acagggcgtt gaggttttta cgcatactgc gtaaattgaa aaacagcttg cgttgcggtt
      721 tggtggtggc aacgcgaacc aaacgccagt ttctcaaaat aaactcgtgg atttcggctt
      781 taatccagtg tacggcaaat gaaaacagac gcgcgcctct accgggctcg tagcgtttga
      841 ccgccttcat cagtccgata ttgccttcct gaatcaggtc tgcctgattc agcccgtagc
      901 cgtcatagcc gcgcgcgatg gaaacgacga cgcgcaggtg ggacaggatg agttgtttgg
      961 cggcgttgag gtcgcccttt atccggcgtt cggcgaggcg ggtttcttct tcctgggaca
     1021 gcatcgggat gctgttgacg gtgtggatgt attgttcgag gctgccgttg ccgctttgga
     1081 tggcgggtaa tgcgaaagcg ttattcattt ggggcatttc ctttcggctg aaactgcgta
     1141 tcggcggttt gctgtgttgg gatgcagtat atcactgctt ggcttgtatt ttgtatgttt
     1201 ggcaggagat atgcgctaag gtttgaaaga caggaaaatt ttgtaaggca ggtttgattg
     1261 attttgtaaa cctgatggct caatccgatt tcggaattat attacatacg tagttgtatg
     1321 taaacagccg gtttgaaaaa agacagcccg tccggacggg ctgtgcaggt atcagtgttc
     1381 tttgtttcgg aagatgaaaa gaatcagtgc ggctagggtc aatatgccca tcaaccacca
     1441 tgaactgccg gttttcatat agggtgtttc gccgacatag cctttgatgt gtccttccaa
     1501 tacggtttcc gtatcgggtt gggcttgggc gatgatgttg cctttggggg agatgatggc
     1561 ggttgcgccg gtgttggtgg cacggaccat atagcgtccg agttccatag cccgtgcctg
     1621 cgattgttgg aggtgctggt acatggcgtt ggattttccg taccacgcca tattgctggc
     1681 attggcaagc agggtggcat ctttggcggc ggcaatcagt tcgtcgccga atccgtcttc
     1741 gtaacagatg ttgaaggcga ttttttggtt tttcatcagc agggcggatt gcttaccgcc
     1801 gcctttgcgg aagtcggaaa ggggcatatt catcattttg taaagcggcg tggtcaggaa
     1861 gggcagcggt ttgtattcgc cgaaggggac gaggtggttt ttggcgtagt aggggatgcc
     1921 gtcctgattg ttttcctgat aaccggtcag gttgatgacg gcgttttcgt aaccgttgcc
     1981 gtccgaagtg tattggctga tgccgacggc gagcgcgctg ccgttgtttt gcgcctgttc
     2041 ggcaaatttt gccagtatgt tttccggcag gttttggcgc ataacgggga tggcggtttc
     2101 gggcaggatg acgatgtcgg cagtggtttt gccgacctgt tcgtaatatt tctgtatggt
     2161 cgggatgact tggtcttcac gccatttgag ggtttggtcg atgttgcctt gaagcagggc
     2221 gacggtgctg cggctgccgt cggggcgggt gaagtcggtt tgtcgggcgg tgtagcctgc
     2281 ggcaagcagg gcggcaatca ggataattgg aagcaggcgt ttgcccgaac gtgcggtgtt
     2341 atcactcgcc aaaaccagcc agacaccgag aaaggcggtt gccagtgtaa ccatgtggat
     2401 gccgcccaat ggggcaaagc cggcgagcgg gctgtccggg gtgatttggg agtagccgat
     2461 tgcgccccag ccgaatccgg tcaggaaacg ctcgcgggca aactcggtca gcgtccacag
     2521 gatgggcagt accaaaccga tttttatgcc ccgaggcagg gtaaattttt tccacagcca
     2581 gaaacacagt gccggataaa gggcaaggta ggcggggagt aggaaggtca gcggtacggc
     2641 atagaggtcg ggcaggccgg aaacgtcgtg cagggcggtg tgtatccagt agaactgtgt
     2701 cgtgtatgcg gtcaggccga acaggtaggc ggaagagaca gcaaaacgcg gacgcagttc
     2761 gatgaggcgg acgaaggcac cgaaaatcaa gggcatcagc caaaagtggt agtagggtgc
     2821 gaaggtaaag ggggtggcgg cggcaaaaag gatgagcaaa ggccagtaga gggcggggtg
     2881 ctgccagtat ttgtccagtt tggaaaccat attcatctgt ctgttcggaa gataccgtct
     2941 gaacatcttt caaacggcat cggtatttga aaaagaaatc aatgcctgcc gaaacgattc
     3001 atcagcggca aggcgggggc gcaggcaatc gaaccggaaa gcagcccgac aacgaggttg
     3061 gcgaagtact ccgcccagcc agcgtcccag tgttgcgcgt gcaggaaatc gtgcagaagg
     3121 ccgaggttgt gggcgaccag tacgccgccg ataagaaaca tggcaagcgt gccgaccacg
     3181 ctcaaaccgc gcataaagca aggcataaag gcagtcagca tttgccccaa actgcgcgaa
     3241 aaggtttgtg ggcggcgcat cagcagcatg cctaagtcgt cgagtttgac gatgacggca
     3301 acgattccgt acaccaaaac agtcatgccg atgccgattg ccgccattac gagcatgcgc
     3361 gtcatgtcgg tgcagaaact tgtgcagcag cttttctacg ccttcaaagc acagataaat
     3421 gccgcctgcc gtcaaaagcg gcgtaatgag ttgcggcagg aaggcggaaa gcagcagggc
     3481 cgcaggcacc aaaaccggct tgttggaaaa agaacctttc gccatcgacc aaacaatcgg
     3541 caactcgcgt tctgccgata cgcccgtaac ccggttggca ttgggtgcca aatcgtcgcc
     3601 gaccatgccg gcggttttct ttgcggcggc tttggtcatc agggcaacat cgtccaaaac
     3661 ggcggtgatg tcgtccggca gggtaaatag tgaggcaaat gccattaaag aatcctgaaa
     3721 tgcggcgcaa agtccgacat tatataggag aacgcggatt tgggcggttt caggcggcat
     3781 gaaacaggaa aatgccgtct gaaggcggtg gcggacgtga agtaaagttt cgtgaaaaaa
     3841 atgccgtgtt acagtctttc gattttaatt taatgaattt taagggagaa tcgttagcgt
     3901 ggattggatg ggcagtctgt tcctgccggg tggcgcactg ttgttcctga gcgtggtttc
     3961 gaccactttg tccgcatgtt tgggaatgcc tttgctgctg gtttctcccg ccggcgtgtt
     4021 ggacggggcg gcggaagcct tggcgattgc ggcgttcctg atgctggtcg cgcgtccgcc
     4081 ggcagtgttc ggcggtttgt ggaaattcaa ttacagc
//
LOCUS       DAWHSK010000179         4077 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.179, whole genome shotgun sequence.
ACCESSION   DAWHSK010000179 DAWHSK010000000
VERSION     DAWHSK010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4077
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.179"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(213..2009)
                     /locus_tag="WIC03_001645"
     CDS             complement(213..2009)
                     /locus_tag="WIC03_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEZ3884117.1"
                     /translation="MNTVSNYLSALREAMRAQGLDALVIPSADPHLSEYLPEHWQARR
                     ELSGFTGSVGTFVVTADEAGVWVDSRYWEQAAKQLAGSGIELQKSGQVPPYNEWLAAN
                     LPENAAVGIPSDMVSLTGKRTLAQSLTAKNIRIEHPNDLLDQVWTSRPAIPAETVFIH
                     DPAYVSETAAEKLARVRAVMAEKGADYHLVSSLDDIAWLTNLRGSDVPFNPVFVSFLL
                     IGKDNAVLFTDRCRLNAEAAAALQTAGITVEPYAQVADKLAQIGGALLIEPNKTAVST
                     LVRLPESVRLIEGINPSTLFKSCKSEADIARIREAMEHDGAALCGFFAEFEDIIGNGG
                     SLTEIDVDTMLYRHRSVRPGFISLSFDTIAGFNANGALPHYSATPESHSTISGNGLLL
                     IDSGAQYKGGTTDITRVVPVGTPTAEQKRDNTLVLKAHIALAEAVFPENIPSPLIDAI
                     CRKPLWQAQCDYGHGTGHGVGYFLNVHEGPQRIAFAAPTTPETAMKKGMVTSIEPGLY
                     RPGKWGIRIENLAANQAVAAPQETEFGSFLCFETLTLCPIDTRLMDTALMTDGEIDWV
                     NRYHAEVRRRLEPLTEGAAKAWLIKRTEPLAR"
     gene            2065..2406
                     /locus_tag="WIC03_001646"
     CDS             2065..2406
                     /locus_tag="WIC03_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884118.1"
                     /translation="MPSEKHNHLIKSPACCKTDGLIGWCVFARKKRCKVVCCKILGIK
                     CRFMPFFGSNMENIVKFEHEVLIVCVGRYLDYVTIRAFIKISLPGGILFLKTVGFGFV
                     WGQSRPNWSNR"
     gene            2467..3720
                     /locus_tag="WIC03_001647"
     CDS             2467..3720
                     /locus_tag="WIC03_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEZ3884119.1"
                     /translation="MSEQHISTWKSKINALGPGIMMASAAVGGSHLIASTQAGALYGW
                     QIALIIILTNLFKYPFFRFSAHYTLDTGKSLIEGYAEKSRVYLWVFLILCILSATINA
                     GAVAIVTAAIVKMAIPSLMFDVGTVAALIMASCLIILVSGRYRALDRVSKIIIVTLSI
                     ATLAAAGIAMSRGMQMQPDFIEPTPWTLAGLGFLIALMGWMPAPIEISAINSLWVTEK
                     QRINPSEYRDGIFDFNVGYIASAVLALVFLALGAFVQYGNGEAVQMAGGKYIGQLINM
                     YAVTIGGWSRPLVAFIAFACMYGTTITVVDGYARAIAEPVRLLRGKDKTGNAEFFAWN
                     IWVAGSGLAVIFWFDGVMANLLKFAMIAAFVSAPVFAWLNYRLVKGDEKHKLTSGMNA
                     LALAGLIYLTGFTVLFLLNLAGMFK"
     gene            3875..>4077
                     /locus_tag="WIC03_001648"
     CDS             3875..>4077
                     /locus_tag="WIC03_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEZ3884120.1"
                     /translation="MSEQNHPQTEPQLDENQIIALRREKLHNIRQQRNAYPNDFKRDS
                     FAADLHAQYGEIGKEELDPQGIPV"
BASE COUNT          811 a         1005 c         1248 g         1013 t
ORIGIN      
        1 cgccttagct caaagagaac gattctctca ggtgctgaag caccgagtga atcggttccg
       61 tactattcgt actgtctgcg gcttcgtcgc cttgtcctga tttttgttaa tccactatat
      121 ttgcttgcgg caagatgaaa acggtgcggg atgttttggg aaaccaatgc cgtctgaagg
      181 gctttcagac ggcatttttt gcgccgtgct gtttaacgcg ccagcggttc ggtgcgtttg
      241 atcagccacg cttttgccgc gccttcggtc agcggctcga ggcggcggcg gacttcggcg
      301 tggtagcggt tgacccagtc gatttcgccg tcggtcatga gggcggtgtc catcaggcgg
      361 gtgtcgatgg ggcagagggt cagggtttca aaacagagga agctgccgaa ttcggtttct
      421 tgaggggcgg cgacggcttg gttggcggca aggttttcaa tgcggatgcc ccattttccc
      481 gggcggtaga gtccgggttc gatggaggta accatgcctt ttttcatggc ggtttcgggc
      541 gtggtggggg cggcgaaggc gatgcgctgc gggccttcgt ggacgttgag gaaatagcct
      601 acgccgtgtc cggtgccgtg gccgtagtcg cattgcgcct gccacagggg tttgcggcaa
      661 atcgcatcaa tcagcggcga ggggatgttt tcggggaaca cggcttcggc aagcgcgata
      721 tgggctttga gaacgagggt gttgtcgcgt ttttgttcgg cggtgggtgt gccgacaggg
      781 acgacgcggg tgatgtcggt cgtgccgcct ttgtattgcg cgccggagtc gatgagcaaa
      841 agcccgttgc cgctgatggt gctgtggctt tcgggtgtcg cgctgtaatg cggcagtgcg
      901 ccgttggcgt tgaagcctgc gatggtgtcg aaactcaatg aaatgaagcc tgggcgcacg
      961 ctgcggtggc gataaagcat ggtgtccacg tcgatttcgg tcaggctgcc gccgttgccg
     1021 atgatgtctt caaactcggc gaagaaaccg cacaacgccg cgccgtcgtg ttccatcgct
     1081 tcgcggatgc gggcgatgtc ggcttcggat ttgcaggatt tgaacagcgt ggacgggttg
     1141 attccctcga taaggcgcac gctttcgggc aggcgcacaa gcgtgctgac ggcggttttg
     1201 ttcggttcga taagcaacgc gccgccgatt tgcgcgagtt tgtcggcaac ttgggcgtaa
     1261 ggctcgaccg tgatgcctgc ggtttgcagc gcggcggcgg cttcggcgtt cagacggcat
     1321 cggtcggtaa acaggacggc gttgtctttg ccaatcagca ggaaggacac gaaaacgggg
     1381 ttgaaaggca cgtcgctgcc gcgcaggttg gtcagccagg cgatgtcgtc aagcgaggaa
     1441 accaagtggt aatccgcgcc tttttccgcc atgacggcgc gcacgcgggc gagtttttcg
     1501 gcggcggttt cggagacgta ggcggggtcg tggatgaaca cggtttcggc ggggatagcg
     1561 ggacggcttg tccacacttg gtcaagcaga tcgtttgggt gttcgatgcg gatgtttttg
     1621 gcggtcaggg attgcgccaa agtgcgtttg ccggtgagcg agaccatatc ggaagggatg
     1681 ccgacggcgg cgttttcggg caggtttgcc gcgagccatt cgttatacgg cggcacttgc
     1741 ccgctttttt gcagttcgat gccgctgccc gcaagctgct tggcggcttg ttcccaatag
     1801 cggctgtcca cccatacgcc cgcttcatcg gcggtaacga cgaatgtgcc gaccgagccg
     1861 gtaaagcccg acaattcgcg gcgcgcctgc caatgctcgg gcaggtattc ggacaggtgg
     1921 gggtcggcgg aagggatgac gagtgcgtcc aagccttgcg ccctcatggc ttcgcgcaat
     1981 gcggacaggt aattcgatac ggtattcata atagttcctc caatcgggtt ttgcggcttc
     2041 agacggcatg gacggaaatc tgaaatgccg tctgaaaaac ataaccatct tatcaaatcg
     2101 ccagcctgct gcaaaacgga cgggctgatc ggatggtgcg tgtttgcgcg aaaaaagcga
     2161 tgtaaggttg tttgttgtaa aattttaggc ataaaatgtc gtttcatgcc tttttttgga
     2221 agtaatatgg aaaatatagt caagtttgag catgaagttc taatagtgtg tgttggcagg
     2281 tatttggatt acgttacaat acgcgctttc atcaaaattt ccctaccggg cggtatcctg
     2341 tttttgaaga cggtaggatt tgggtttgtt tgggggcaat cccgcccaaa ttggagcaat
     2401 agatagggct gtgtccgaat atgcggctct gtgtgttgaa acacggttaa aaatacggaa
     2461 gactttatgt ccgaacaaca tatttcgact tggaaaagta aaatcaacgc attgggtccg
     2521 gggatcatga tggcttcggc ggcggtcggc ggttcgcacc tgattgcctc gacgcaggcg
     2581 ggcgcgcttt acggctggca gatcgcgctc atcatcatcc tgaccaacct cttcaaatac
     2641 ccgtttttcc gcttcagcgc gcattacacg ctggacacgg gcaagagcct gattgaaggt
     2701 tatgccgaga aaagccgcgt ttatttgtgg gtattcctga ttttgtgcat cctctccgcc
     2761 acgattaacg cgggcgcggt cgccattgta accgccgcca tcgtcaaaat ggcgattccc
     2821 tcgctgatgt ttgatgtcgg cacggttgcc gccttgatta tggcatcctg cctgattatt
     2881 ttggtgagcg gacgttaccg cgctttggat cgcgtttcca aaatcatcat cgttactttg
     2941 agtatcgcca cgcttgctgc cgccggcatc gctatgtcgc gcggtatgca gatgcagccc
     3001 gattttatcg agccgacacc gtggacgctt gccggtttgg gcttcctgat cgcgctgatg
     3061 ggctggatgc ccgcgccgat tgaaatttcc gccatcaatt ctttgtgggt aaccgaaaaa
     3121 caacgcatca atccttccga ataccgcgac gggatttttg atttcaacgt cggttatatc
     3181 gccagtgcgg ttttggcttt ggttttcctt gcactgggtg cgtttgtgca atacggcaac
     3241 ggcgaagcag tgcagatggc gggcggcaaa tatatcgggc aattgatcaa tatgtacgcc
     3301 gttaccatcg gcggctggtc gcgcccgctg gtggcgttta tcgcgtttgc ctgtatgtac
     3361 ggcacgacga ttaccgtcgt ggacggctat gcccgtgcca ttgccgaacc cgtgcgcctg
     3421 ctgcgcggaa aagacaaaac gggcaacgcc gaattctttg cctggaatat ttgggtggcg
     3481 ggcagcggtt tggcggtgat tttttggttt gacggcgtaa tggcgaatct gctcaaattt
     3541 gcgatgattg ctgcttttgt gtccgcccct gtgtttgcct ggctgaatta ccgtttggtc
     3601 aaaggtgatg aaaaacacaa actcacatca ggtatgaatg cccttgcatt ggcaggcttg
     3661 atttatctga ccggttttac cgttttgttc ttattgaatt tggcgggaat gttcaaatga
     3721 taacgatgcc gtctgaaacc gcaaaccgct ttcagacggc attgtcgcgt ttatggaaaa
     3781 aaatgccgac atcgcgtaaa atatgcgcaa attttgtgat tcggtcaggt cgtctgaaac
     3841 agattgcgcc tgatttattt tttcggaaaa ccctatgagc gaacaaaacc atccgcaaac
     3901 cgagccgcag ttggacgaaa accaaatcat cgccctgcgc cgcgaaaaac tgcacaacat
     3961 ccgccaacag cgcaacgcct atcccaacga cttcaaacgc gacagcttcg ccgccgattt
     4021 gcacgcccaa tacggcgaaa tcggcaaaga agaactcgat ccgcaaggca ttcccgt
//
LOCUS       DAWHSK010000180         4077 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.180, whole genome shotgun sequence.
ACCESSION   DAWHSK010000180 DAWHSK010000000
VERSION     DAWHSK010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4077
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.180"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..1237
                     /locus_tag="WIC03_001649"
     CDS             <1..1237
                     /locus_tag="WIC03_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEZ3884121.1"
                     /translation="LARHADVTLFEAGRQAGGRARTLAGNTDGFGFLDNGQHILLGAY
                     RGVLRLMKTIGSDPRAAFLRVPLHWHMHGGLQFRALPLPAPLHILGGVLLARRAPTAF
                     KAKLLADMSDLQKSARLGQPDTTVAQWLKQRNVPRAAVMQFWQPLVWGALNTPLETAS
                     LRVLCNVLSDGVLTKKSGSDYLLPKQDLGAIVAEPALADLQRLGADIRLETRVCRLNT
                     LPDGKVLVNGEAFDAAVPATAPYHAAALLPEGTPEHVQTAYQNLRYHAITTVYLRYAE
                     PVRLPAPLTGLADGTVQWLLCRGRLGLPENEVSAVISVSDRVGAFANRAWADKAHADL
                     KRILPHLGEPEAVRVITEKRATTAADAPPPDLSWLHRHRIFPAGDYLHPDYPATLEAA
                     VQSGFAAAETCLQSLSDAV"
     gene            1321..2040
                     /locus_tag="WIC03_001650"
     CDS             1321..2040
                     /locus_tag="WIC03_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEZ3884122.1"
                     /translation="MATLSNKTILVTGASQGLGEQVAKAYAAAGATVILVARHQKKLE
                     KVYDAIVEAGHPEPFAIRFDLMSAEEKEFNQFATTIAEATQGKLDGIVHCAGYFYALS
                     PLDFQTVAEWVNQYRINTVAPMGLTRALFPLLKQSPDASVIFVGESHGETPKAYWGGF
                     GASKAALNYLCKVAADEWERFGNLRANVLVPGPINSPQRIKSHPGEAKSERKSYGDVL
                     PAFVWWASAESKGRSGEIVYL"
     gene            2205..2876
                     /locus_tag="WIC03_001651"
     CDS             2205..2876
                     /locus_tag="WIC03_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HEZ3884123.1"
                     /translation="MKTVSTAVVLAAAAVSLTGCATESSRSLEVAKVASYNTQYHGVR
                     TPISVGTFDNRSSFQKGIFSDGEDRLGSQAKTILVTHLQQTNRFNVLNRTNLNALKQE
                     SGISGKAHNLKGADYVVTGDVTEFGRRDVGDHQLFGIFGRGKSQIAYAKVALNIVNVN
                     TSEIVYSAQGAGEYALSNREIIGFGGTSGYDATLNGKVLDLAIREAVNSLVQAVDNGA
                     WQPNR"
     gene            2886..3254
                     /locus_tag="WIC03_001652"
     CDS             2886..3254
                     /locus_tag="WIC03_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4810 domain-containing protein"
                     /protein_id="HEZ3884124.1"
                     /translation="MNPKTLSRLSLCAAVLALTACGGNGQKSLYYYGGYPDTVYEGLK
                     NDDTSLGKQTEKMEKYFVEAGNKKMNAAPGAHAHLGLLLSRSGDKEGAFRQFEEEKRL
                     FPESGVFMDFLMKTGKGGKR"
     gene            3251..3898
                     /locus_tag="WIC03_001653"
     CDS             3251..3898
                     /locus_tag="WIC03_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF799 domain-containing protein"
                     /protein_id="HEZ3884125.1"
                     /translation="MKPLILGLAAVLALSACQVQKAPDFDYTSFKESKPASILVVPPL
                     NESPDVNGTWGVLASTAAPLSEAGYYVFPAAVVEETFKQNGLTNAADIHAVRPEKLHQ
                     IFGNDAVLYITVTEYGTSYQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSN
                     SGLLGALVSAVVNQIANSLTDRGYQVSKTAAYNLLSPYSHNGILKGPRFVEEQPK"
BASE COUNT          944 a         1270 c         1064 g          799 t
ORIGIN      
        1 cttggcgcgg cacgccgacg ttaccctgtt tgaagccggc cggcaggcgg gcggcagggc
       61 gcgcacactg gccggaaata ccgacggttt cggttttttg gacaacgggc agcacatttt
      121 gctcggcgca taccggggcg tgttgcgcct gatgaaaacc atcggttcgg atccccgtgc
      181 cgcctttttg cgcgtaccgc tgcactggca tatgcacggc ggtttgcagt tccgcgccct
      241 ccccctgccc gcgccgctgc atattttggg cggcgtgctg cttgcccggc gtgcaccgac
      301 tgcattcaaa gccaaactgc ttgccgatat gtccgatttg cagaagtccg cacgcctcgg
      361 acagcccgac acgacagtgg cgcaatggct gaaacagcgg aacgtgccgc gtgccgccgt
      421 gatgcagttt tggcagccct tggtttgggg cgcgctcaac acgcctttgg aaaccgcaag
      481 cctgcgcgtg ttgtgcaacg ttttgtccga cggcgtgctg acgaaaaaat ccggcagcga
      541 ctatctccta cccaaacagg atttgggcgc aatcgtcgcc gaacccgcct tggcggatct
      601 tcaacggctc ggcgcggaca tccgcctcga aacgcgcgta tgccgtctga acaccctccc
      661 ggacgggaaa gtgctcgtca acggcgaagc tttcgatgcc gccgtccccg ccaccgcgcc
      721 ctaccacgcc gccgcgctcc tgcccgaagg cacgcccgaa cacgttcaga cggcatatca
      781 aaaccttcgc taccacgcca tcaccaccgt ctatctgcgc tacgccgaac ccgtccgcct
      841 gcccgccccg ctgaccggcc ttgccgacgg cacggtgcaa tggctgcttt gccggggcag
      901 gctcggactg cctgaaaacg aagtgtccgc cgtcatcagc gtttccgacc gcgtcggcgc
      961 gtttgcaaac cgggcgtggg cggacaaagc ccacgccgac ctcaaacgca tccttccgca
     1021 tttgggcgaa cccgaagccg tgcgcgtcat caccgaaaaa cgcgccacaa ccgcagccga
     1081 tgccccgccg ccggatttgt cgtggctgca ccggcaccgc atcttccccg ccggcgacta
     1141 cctccacccg gactaccccg ccacgctcga agccgccgta caatcaggtt tcgcggcggc
     1201 ggaaacctgc ctgcaaagcc tgagcgatgc cgtctgaaaa cgccggctgg tatcgggacg
     1261 ctttacaagg tgcggcaaaa cgctaaaata cccccgaccg accaccaaaa aggaaaaagt
     1321 atggcgacac tgtccaacaa aaccatctta gttaccggcg catcgcaagg tctgggcgaa
     1381 caggtcgcca aagcctatgc ggcggcaggc gcaaccgtga ttctggtggc acgccatcag
     1441 aaaaaactgg aaaaagtcta tgacgcgatt gtcgaagccg gacaccccga acccttcgcc
     1501 atccgtttcg acctgatgag tgcggaagaa aaagaattca atcagttcgc caccaccatt
     1561 gccgaagcca cgcaaggcaa actggacggc atcgtccact gcgccggcta tttttacgcc
     1621 ctctcgccgc tggatttcca aaccgtcgcc gaatgggtca accaataccg catcaacacc
     1681 gtcgcaccta tggggctgac ccgcgccctg ttcccgctgc tgaagcagtc gcccgacgcg
     1741 tccgtcatct tcgtcggcga aagccacggc gaaacaccca aagcctactg gggcggcttc
     1801 ggcgcgtcca aagccgcgtt gaactacctg tgcaaagtcg ccgccgacga atgggaacgc
     1861 ttcggcaacc tgcgcgccaa cgtcctcgtc cccggaccca tcaattcccc gcaacgcatc
     1921 aaatcccatc cgggcgaagc caaaagcgaa cgcaaaagct acggggacgt gctgcccgca
     1981 tttgtctggt gggcaagtgc cgaaagcaaa gggcggagtg gcgaaatcgt ttacctctaa
     2041 atccggaaaa ggcaggcatc ccgcccgcgc cgcgattccg tttgaaaaat atccgtacct
     2101 tccgcgcgca tccgtcccgc acagacaaac accttgactt aaatttacat aaggacaata
     2161 atgacaaccc gttatttcct taacttgacc ttagggtaaa ttttatgaaa accgtttcca
     2221 ccgccgttgt ccttgccgcc gctgccgttt cactgaccgg ctgtgcgacc gaatcctcac
     2281 gcagtctcga ggtagcaaaa gtcgcctcct acaatacgca atatcacggt gttcgtaccc
     2341 cgatttccgt cggaacattc gacaaccgct ccagcttcca aaaaggcatt ttctccgacg
     2401 gggaagaccg tttgggcagc caggcaaaaa ccattctggt aacgcacctg caacagacca
     2461 accgcttcaa cgtactgaac cgcaccaatt tgaacgcatt aaaacaggaa tccggcattt
     2521 ccggcaaagc gcataacctg aaaggcgcag attatgtcgt taccggcgat gtaaccgaat
     2581 tcggacgcag agatgtcggc gatcatcagc tcttcggcat ttttggtcgc ggcaaatcgc
     2641 aaatcgccta tgcaaaagtg gctctgaata tcgtcaacgt caatacttcc gaaatcgtct
     2701 attccgcaca gggcgcgggc gaatacgcac tttccaaccg tgaaatcatc ggtttcggcg
     2761 gcacttccgg ctacgatgcg actttgaacg gcaaagtttt agacttggca atccgcgaag
     2821 ccgtcaacag cctggttcag gctgttgaca acggcgcatg gcaacccaac cgttaaggag
     2881 aaatgatgaa tccgaaaacc ttgagccgtt tgtcgctgtg tgcggcggtc ttggctctga
     2941 ccgcctgcgg cggcaacggg caaaaatccc tgtattatta cggcggctat cccgataccg
     3001 tctatgaagg tttgaaaaac gacgacactt cgttgggcaa gcagaccgaa aagatggaaa
     3061 aatactttgt ggaagccggc aacaaaaaaa tgaatgccgc cccgggtgcg cacgcccatc
     3121 tgggactgct gctttcccgt tcgggagaca aagagggcgc gttccgccag tttgaagaag
     3181 agaaaaggct gtttcccgaa tcgggcgtat ttatggactt cctgatgaaa accggtaaag
     3241 gaggcaagcg atgaaacctt tgattttagg gcttgccgcc gtgttggcgc tgtctgcctg
     3301 ccaagttcaa aaagctcccg acttcgacta cacgtcattc aaggaaagca aaccggcttc
     3361 aattttggtg gttcccccgc tgaacgaatc gcccgatgtc aacggaacat ggggtgtact
     3421 ggcttcgacc gccgctccgc tttccgaagc cggctattac gtcttccccg ccgcagtcgt
     3481 ggaggaaacc ttcaaacaaa acggcttgac caatgccgcc gatattcacg ccgtccggcc
     3541 ggaaaaactg catcagattt tcggcaatga tgcggttttg tacattacgg ttaccgaata
     3601 cggcacttca tatcaaattt tagacagcgt gacgaccgta tccgccaaag cacggctggt
     3661 cgattcccgc aacggaaaag agttgtggtc gggttcggcc agcatccgcg aaggcagcaa
     3721 caacagcaac agcggcctgt tgggggcttt ggtcagcgca gtggtcaatc agattgccaa
     3781 cagcctgacc gaccgcggtt atcaggtttc caaaaccgcc gcatacaacc tgctgtcgcc
     3841 ctattctcac aacggcatct tgaaaggtcc gagattcgtc gaagagcagc ccaaataagc
     3901 atatgccgtc tgaacctctc cgagcttcag acggcatatg cgaatcttga tacttgcaag
     3961 ccgcaccgcc caaaatcggg cagccggttt caatatccga acaaggttcg ggttctgtat
     4021 tttgcagaac ccgaaccttt tattcaaatt taaacttaat tccggcgtgg ttgtatg
//
LOCUS       DAWHSK010000181         4077 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.181, whole genome shotgun sequence.
ACCESSION   DAWHSK010000181 DAWHSK010000000
VERSION     DAWHSK010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4077
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.181"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            162..947
                     /locus_tag="WIC03_001654"
     CDS             162..947
                     /locus_tag="WIC03_001654"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEZ3884126.1"
                     /translation="MSRIRQAFAALDGGKALIPYITVGDPDIGATLAIMHSMVENGAD
                     ILELGVPFSDPMADGPVIQRAAERALANGISLRDVLDVVRKFRETDTQTPVVLMGYLN
                     PVHKMGYREFAQEAAMAGVDGVLTVDSPVETIDPLYRELKDNGVDCIFLIAPTTTEDR
                     IKTIAELAGGFVYYVSLKGVTGAASLDTDEVSRKIEYLHQYIDIPIGVGFGISNAESA
                     RKIGRVADAVIVGSRIVKEIENNAGNEAAAVGALVKELKDAVR"
     gene            984..1856
                     /locus_tag="WIC03_001655"
     CDS             984..1856
                     /locus_tag="WIC03_001655"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEZ3884127.1"
                     /translation="MSWLDKILPPKIKNRSKDGSSNVPEGLWHKCPSCSATVYSTELQ
                     QNNQVCPKCNHHNPLSARQRLNLLLDEDGREEVAGNVKPTDPLKFKDGKKYPDRLSAA
                     RKLTGEDDALVVMKGKMNGLPVVVAAFEFRFIGGSMGSVVGERFVQGIRRAVADNCPF
                     VCVAASGGARMQEGVNSLMQMTKTSAALHLLTEKRLPFISVLTDPTMGGVSASFAFLG
                     DVVLAEPNALIGFAGPRVIEQTVRETLPEGFQRAEFLLEKGAIDQIVDRREMKQRISG
                     LITLLCRQDKVSAA"
     gene            complement(1916..2527)
                     /locus_tag="WIC03_001656"
     CDS             complement(1916..2527)
                     /locus_tag="WIC03_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNP1-like family protein"
                     /protein_id="HEZ3884128.1"
                     /translation="MRRTALLLLALTAGTSLAAGFNQKDTPINTRYRETPEEAAAREF
                     KEHTAKLPPLPDTHSDGWFDIYVDENYGKQPKILLDSLQIMPAPDGSIRYILNIRSDK
                     GYDNLTAEGIFCARFSIRFGNGKPSSYKVFGYGDTVNRRWIQPRNAEWKPIGGTLSRN
                     DALRTVLYQAFCEGGVPADTKGLVQRLKERAGRYAPSMKPHDK"
     gene            2636..2860
                     /locus_tag="WIC03_001657"
     CDS             2636..2860
                     /locus_tag="WIC03_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEZ3884129.1"
                     /translation="MNSETLDVTGLKCPLPILRAKKALAQMQQGDVLTVLATDGGAPG
                     DFEAFCRQTGHVLLDASEQDGVFTLVVKHK"
     gene            2861..3466
                     /locus_tag="WIC03_001658"
     CDS             2861..3466
                     /locus_tag="WIC03_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884130.1"
                     /translation="MPSEMRMSRLMLLLSAFVLAACNAQNRQSCVRHQDFARVAVDLF
                     RSACVLTQGSFEPVSKFAAVGHFVPVGKEDLSRLPPAVAEPDVQALWTLERDGGIYYL
                     SLPRDCCSVKAERADSAVLLEHFDGLVRQPPKNTNIELRAEQSVQALFETRQISYAWR
                     ESGSPEETVLTAQTADSPDLPVQAVLNLTHRSHNGKPLILP"
     gene            3526..>4077
                     /gene="pyrC"
                     /locus_tag="WIC03_001659"
     CDS             3526..>4077
                     /gene="pyrC"
                     /locus_tag="WIC03_001659"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEZ3884131.1"
                     /translation="MQTLTIIRPDDMHLHLRDGDALKAVVPYTARQMGRAVIMPNLKP
                     PVVSVADALAYKARIMAALPEGSAFEPLMTLYLTDNATSELVREAKAAGIVAFKLYPA
                     GATTNSDSGVTDLFKLIPVLEEMAKQGILFLVHGEVTDPEIDIFDREAAFIGRVMKPV
                     LAQVPNLKVVFEHITTAEAARLVL"
BASE COUNT          883 a         1002 c         1198 g          994 t
ORIGIN      
        1 tccgagatgc ttgccgttta tttccgcctc gttcaaacgg cggctctgat ttgcgcggtt
       61 tctgtttgcc gtattcgcct atccgtaccg caaatgttat actgggaaaa atttactgat
      121 tgtgttttac ggcatatttg ccgataggat ggaagagaca aatgagcaga atccggcagg
      181 cttttgccgc tttggatggc ggaaaggcat tgattcccta tattacggtt ggagacccgg
      241 atattggggc gactttggca ataatgcaca gtatggtgga aaacggtgcg gatattttgg
      301 agttgggcgt gccgttttcc gatccgatgg cggatggacc ggttattcag cgtgcggcgg
      361 agcgggcgtt ggcgaacggg atttcgctgc gcgatgtctt ggatgtcgtc agaaaattcc
      421 gtgaaaccga cacgcaaact ccggttgttt tgatgggata tttgaaccct gtacataaga
      481 tgggttatcg ggagtttgct caggaagccg caatggcggg tgtggacggc gtgttgacgg
      541 tggattcccc tgtcgaaacc atcgatccgc tctatcgcga gctgaaggat aacggggtcg
      601 actgtatttt cctgattgcg ccgacgacga cggaagaccg tattaaaacc attgccgagc
      661 tggcaggcgg atttgtctat tatgtttcgc tcaagggcgt aacgggcgcg gcaagtttgg
      721 atacggatga ggtttcgcgt aaaatagagt atttgcatca gtatatcgat attcccatcg
      781 gtgtcggttt cggcatcagc aatgcggaaa gcgcgcgcaa aatcggccgg gttgccgacg
      841 cggtcattgt cggcagccgg attgtgaaag agattgaaaa caatgcgggc aacgaggctg
      901 cggccgtcgg tgctttggtc aaagagttga aggatgccgt gcgctgattc tgtcacgcat
      961 tctaaatatt ttaggagttg tccatgagct ggttagataa aatcctgcca cccaaaatca
     1021 aaaaccgcag caaagacggt tcttccaatg ttcccgaggg tttgtggcac aaatgtccgt
     1081 cttgttcggc aaccgtttat tcgaccgagt tgcagcagaa caatcaggtt tgtccgaaat
     1141 gcaaccacca caatccgttg tcggcacgac aacgcctgaa tctgcttttg gatgaggatg
     1201 gcagagagga ggttgccgga aatgtcaaac cgacagatcc tttgaagttt aaagacggca
     1261 aaaaatatcc ggatcgtttg agtgcggcac gcaagctgac cggggaagat gatgctttgg
     1321 tggtaatgaa aggcaagatg aacggtctgc ccgttgtggt tgctgcgttt gagttccgct
     1381 ttatcggcgg ttcgatgggt tcggttgtgg gcgaacgatt cgtacaaggt atccgtcggg
     1441 cggttgccga caattgtccg ttcgtctgtg tggcggcttc cggcggtgcg cgtatgcagg
     1501 agggtgtgaa ctcgttgatg cagatgacga aaaccagtgc cgcgctgcat ttgctgacgg
     1561 aaaaacgcct gccgtttata tcggtattga ccgatccgac tatgggcggc gtatccgcca
     1621 gcttcgcatt tttgggcgat gtcgtgcttg ccgaacccaa tgcattaatc ggctttgccg
     1681 gtccgcgcgt gattgagcag acggtgcgcg aaacgctgcc ggaaggcttc cagcgtgccg
     1741 agtttttgct ggaaaaaggc gcaatcgacc agattgtcga ccggcgcgag atgaagcaga
     1801 gaatcagcgg tttgattacg ctgttgtgcc gtcaggacaa agtttctgcc gcctgatggc
     1861 tgatgaatcg aataccgtct gaaaccgatg tttcagacgg tatttttgtg tctggttatt
     1921 tgtcgtgcgg cttcatcgac ggggcatagc gtccggcacg ttctttcagg cgttgtacca
     1981 aacctttcgt gtcggcgggt acaccgccct cgcagaatgc ctgatacagg acggtgcgca
     2041 gtgcgtcgtt gcggcttaat gtaccgccta tcggtttcca ttcggcgttt cggggctgta
     2101 tccagcggcg gttgaccgtg tcgccgtagc cgaacacttt ataggaggaa ggtttgccgt
     2161 tgccgaacct gatggagaag cgggcgcaga atatgccttc ggcagtcagg ttgtcgtagc
     2221 ctttgtcgga gcggatattg agaatgtagc ggatgctgcc gtcgggcgcg ggcataattt
     2281 gcaggctgtc gagcaggatt ttcggctgtt tgccgtaatt ttcatccaca taaatgtcaa
     2341 accagccgtc cgagtgcgta tcgggcagcg gcggcagttt ggcggtatgt tctttaaatt
     2401 cgcgggcggc ggcttcttcg ggcgtttcgc ggtagcgggt gttgatcggc gtgtcttttt
     2461 ggttgaagcc ggcagcaagg gacgtgccgg cggtgagtgc gagcagcagg agggcggtgc
     2521 ggcgcataag tttctccaaa ttgaaaacgg cgttatttta tgggttggca aagggggctg
     2581 caagcaactg gggtataatc tccccccgat tcccattttt taaacggtac aaacgatgaa
     2641 cagcgaaact ttagacgtaa ccggattgaa atgtcccctg ccgattttgc gggcgaaaaa
     2701 ggctttggcg caaatgcagc agggcgacgt gttgaccgtt ctggcaaccg acggcggcgc
     2761 accgggggat tttgaggctt tttgccgcca aaccggtcat gtgctgttgg atgcttccga
     2821 acaggacggc gtgttcacgc tggtcgtcaa gcacaaataa atgccgtctg aaatgcggat
     2881 gtcccgattg atgttattgc tgtcggcatt tgtgcttgct gcgtgtaatg cccaaaaccg
     2941 gcaatcctgt gtacggcatc aggatttcgc acgtgtagcc gtcgatttgt ttcggagtgc
     3001 ctgcgtcctg actcaaggca gtttcgaacc agtttccaag tttgccgctg tcggacattt
     3061 tgtccctgtc ggaaaggaag acctgtcccg tctgccgccc gctgttgcag aacccgatgt
     3121 acaggcgttg tggacgcttg aacgcgatgg cggcatttat tatttgagcc tgccgcgcga
     3181 ctgttgcagt gtgaaagccg agcgggcaga cagtgccgtg cttttggaac attttgacgg
     3241 acttgtccga cagccgccga aaaataccaa tatagaattg agggctgaac agtcggtgca
     3301 ggcactgttt gaaaccaggc aaatcagcta tgcctggcgg gaatcgggca gccccgaaga
     3361 aaccgtattg accgcacaaa ccgccgattc cccggatttg cccgtacagg cagttttgaa
     3421 cctgacgcac cgttcccaca acgggaaacc cctaattctc ccctaacttc agacggcata
     3481 aagtcggcat gccgtctgaa cattcaaata aaacaaagga aaaccatgca aaccctgacc
     3541 attatccgcc ccgacgatat gcacctgcac ctgcgcgacg gcgacgcgct caaagccgtt
     3601 gtgccttata ccgccagaca gatggggcgc gccgtgatta tgcccaatct caaaccgcct
     3661 gtcgtcagcg ttgccgacgc gcttgcctac aaagcgcgca ttatggcggc gttgcccgaa
     3721 ggcagcgcgt ttgagccgtt gatgacgctt tatttgactg ataacgccac gtccgaactt
     3781 gtacgcgaag ccaaagctgc cggcatcgtt gccttcaaac tctaccccgc cggcgcgacc
     3841 accaattccg attccggcgt aaccgacctg ttcaaactta tccccgtttt ggaagaaatg
     3901 gcgaaacagg gcatcctgtt ccttgttcac ggcgaagtaa ctgaccccga aatcgacatc
     3961 ttcgaccgtg aagccgcctt tatcgggcgc gtgatgaaac ccgttttggc gcaagtacca
     4021 aatcttaaag tcgtgttcga acacatcacc accgccgaag ccgcccgcct cgttttg
//
LOCUS       DAWHSK010000182         4061 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.182, whole genome shotgun sequence.
ACCESSION   DAWHSK010000182 DAWHSK010000000
VERSION     DAWHSK010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4061
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.182"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(253..324)
                     /locus_tag="WIC03_001660"
     tRNA            complement(253..324)
                     /locus_tag="WIC03_001660"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(289..291),aa:Arg,seq:cct)
     gene            complement(344..790)
                     /locus_tag="WIC03_001661"
     CDS             complement(344..790)
                     /locus_tag="WIC03_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HEZ3884132.1"
                     /translation="MNFSFVPLFLVTLILLGVVSNNNSITISATILLLMQQTALIQFV
                     PLVEKHGLNLGIILLTIGVLSPLVSGKAQVPPVAEFLNFKMISAVFIGIFVAWLAGRG
                     VPLMGQQPVLITGLLIGTVIGVAFMGGIPVGPLIAAGILSFVVGKG"
     gene            complement(795..1769)
                     /gene="ispB"
                     /locus_tag="WIC03_001662"
     CDS             complement(795..1769)
                     /gene="ispB"
                     /locus_tag="WIC03_001662"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HEZ3884133.1"
                     /translation="MLENLPYFQRHLPEDLAKVNEVINRAVQSDVALISQIGTYIISA
                     GGKRLRPIMTILAGKAVGYDDGKLYSLAAMVEFIHTSTLLHDDVVDESDLRRGRATAN
                     NLFGNAAAVLVGDFLYTRAFQLMVDSDSMRVLEVMADATNIIAEGEVMQLMNIGNTDI
                     TEEQYIQVIQYKTAKLFEAAAQVGAILGKASPEHERALKDYGMYVGTAFQIIDDVLDY
                     SGETEETGKNVGDDLAEGKPTLPLIYLMRQGSEQVANDVRTALENADRSYFEKIHDYV
                     VRSDALAYSIGEARKAVDCAVTALDALPDSEVKDAMIQLAKESLVRVS"
     gene            complement(1785..1973)
                     /locus_tag="WIC03_001663"
     CDS             complement(1785..1973)
                     /locus_tag="WIC03_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884134.1"
                     /translation="MVNIRTPLCGTRHCIVICLFCQSLRGSDNHMPSEKMYPDGFADI
                     IARYLNQLSSGICRLMEA"
     gene            1993..2301
                     /gene="rplU"
                     /locus_tag="WIC03_001664"
     CDS             1993..2301
                     /gene="rplU"
                     /locus_tag="WIC03_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEZ3884135.1"
                     /translation="MYAVVKTGGKQYKVSVGEKLKVEQIPAELDSQIELTEVLMIADG
                     ESVKVGAPFIEGAKVTAKVVAHGRGEKVRIFKMRRRKHYQKRQGHRQNFTQIEIVAIA
                     "
     gene            2326..2598
                     /gene="rpmA"
                     /locus_tag="WIC03_001665"
     CDS             2326..2598
                     /gene="rpmA"
                     /locus_tag="WIC03_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEZ3884136.1"
                     /translation="MASKKAGGSTRNGRDSEAKRLGVKAYGNELIPAGSIIVRQRGTK
                     FHAGDNVGMGKDHTLFAKVDGYVEFKTKGALNRKTVSIRPYTGSEE"
     gene            2833..4017
                     /gene="ubiM"
                     /locus_tag="WIC03_001666"
     CDS             2833..4017
                     /gene="ubiM"
                     /locus_tag="WIC03_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-demethoxyubiquinol-8 5-hydroxylase UbiM"
                     /protein_id="HEZ3884137.1"
                     /translation="MSLHSDILVVGAGPAGLSFAAELAGSGLKVTLIERSPLTVLQNP
                     PYDGREIALTHFSREIMQRLGMWDKIPENEIYPLRDAKVLNGRSDYQLHFPQPTEARG
                     EPADCLGYLISNHNIRRAAYEVVSQLDNVSILTDTVVKEVKTSDNEAQVILENGKILT
                     ARLLLAADSRFSQTRRQLGISSDMHDYSRTMFVCRMKHTLSNQHTAYECFHYGRTIAL
                     LPLEEHLTNTVITVDTDKINSVQNLSPEELAASVKEQLKGRLGDMELVSSIHHYPLVG
                     MIAKRFYGKRSALIGDAAVGMHPVTAHGFNLGLSSADILAKLILEAEQRGQDIGASSL
                     LEKYSNKHMLHAHPLYHGTNMMLKLFTNETAPAKLLRGLVLRAGNNFPPLKKLITKQL
                     TG"
BASE COUNT         1148 a         1120 c          885 g          908 t
ORIGIN      
        1 tctgtactgt ctgcggcttc gtcgccttgt cctgatttaa atttaatcca ctatatcata
       61 aaacaacaga tttccaagcc ggaacatctt ttacggagcc tgaaaatcat ttcatgattc
      121 taccgtccta aaggtcggtt tttcaagcag gaagaataaa gttttcagat ggcaaaaaaa
      181 ccctccaaca ctgaaaggcc ttatatcgga aacttcccgc aacacgggaa acagacaaat
      241 gaaatcgtca aacctcgcca acaggaatcg aacctgtatt ttacgcttag gaggcatacg
      301 ttctatccgt tgaactatgg cgagccgaaa tgaaaaggag attttaaccc tttccgacga
      361 caaaagacaa gatgccggcc gcaatcagcg gcccgacagg gataccgccc ataaatgcca
      421 ccccgataac cgtcccgatt aacagccctg taattaaaac aggctgctgt cccatcaaag
      481 gcacgccgcg tcccgccagc caagccacga aaataccgat aaaaacggcg gatatcattt
      541 taaaattcaa aaattcggca acgggaggaa cctgcgcctt tcctgaaacc aacggactca
      601 aaacccctat ggtcaaaaga atgataccga gattcaaccc gtgcttctcg accaacggga
      661 caaactgtat caatgccgtc tgctgcatca gcagcaatat ggttgccgag atggtaatcg
      721 aattgttgtt gctgaccacc cccaacagaa tcagcgtaac cagaaacagg ggaacaaaac
      781 tgaaattcat cgcctcaaga caccctgacc aaagattcct tcgccagctg aatcatggca
      841 tccttcactt cgctgtcggg cagggcatcc aaggcggtaa cggcacaatc gactgctttg
      901 cgcgcctcgc ctatcgaata tgccaacgca tccgaacgga cgacataatc gtggattttc
      961 tcaaaatagc tgcgatctgc attttccaaa gcagtacgca catcgttcgc aacctgttcg
     1021 gaaccctgac gcatcagata aatcaaaggc aaagtcggtt ttccttccgc caaatcgtcg
     1081 ccgacgtttt tgccggtttc ttcggtttcg ccagaatagt ccagcacatc gtcaataatt
     1141 tggaatgccg taccgacata cataccgtag tctttcaacg cccgttcgtg ttcgggggaa
     1201 gccttgccca aaattgcgcc gacttgagcg gcagcttcaa acaattttgc cgttttatat
     1261 tggatgactt ggatatattg ttcttcggta atgtccgtat tgccgatgtt catcagctgc
     1321 atgacttcgc cctcggcaat aatgttggtt gcatccgcca tcacttccaa aacgcgcata
     1381 ctgtccgagt caaccatcag ttgaaaggcg cgcgtgtata aaaagtcgcc aaccaacact
     1441 gccgccgcat tgccgaacag attgtttgcc gttgcccgtc cacggcgcaa atcgctttca
     1501 tcgacgacat cgtcgtgcag cagcgtcgaa gtgtggataa attcgaccat cgccgccagc
     1561 gaatacagtt tcccgtcatc ataaccgacc gccttacccg ccaaaatcgt cataatcgga
     1621 cgcaggcgtt tgccgcccgc gctgatgata tatgtaccga tttgcgaaat cagtgcgaca
     1681 tcggattgca ccgcacggtt gatgacttca ttgactttgg caaggtcttc aggcagatgt
     1741 cgctggaaat agggcagatt ttcgagcatg gcagactctc aggtttacgc ttccatcaaa
     1801 cggcagatac cgcttgaaag ctgattcaaa tagcgggcaa ttatatcagc aaagccatca
     1861 gggtacatct tttcagacgg catatggtta tccgaaccgc gcaaactttg acaaaacagg
     1921 caaataacaa tacaatgccg agttccgcac aatggtgtgc ggatattaac cataaccctt
     1981 atggagttga gtatgtacgc ggtcgtaaaa accggcggca aacagtataa agtttccgtc
     2041 ggcgaaaaat tgaaagtaga acagatacca gccgaactcg acagccaaat cgaactgacc
     2101 gaagttttga tgattgctga cggcgaatct gtaaaagttg gcgcaccctt tatcgaaggt
     2161 gcaaaagtaa cggctaaagt agtggcacac ggtcgtggcg aaaaagtccg catcttcaaa
     2221 atgcgccgcc gcaaacacta ccaaaaacgc caaggccacc gccaaaattt cacccaaatc
     2281 gaaatcgtgg caatcgccta accttaaaac ttaggagcat tacaaatggc aagtaaaaaa
     2341 gcaggcggca gcacccgcaa cggtcgcgat tcagaagcca aacgcttggg cgttaaagcc
     2401 tacggcaacg agctgattcc cgcaggttcc atcatcgtac gccaacgcgg taccaaattc
     2461 cacgcaggcg acaacgtagg catgggcaaa gatcacactt tgttcgccaa agttgacggt
     2521 tatgttgaat tcaaaaccaa aggtgcgctg aaccgtaaaa ctgtcagcat ccgtccttac
     2581 accggttctg aagaataatc caaccgatac ctgaagccgc atcttttcac gatgcggctt
     2641 tttacatacc ccacacccga tttgatacac attaagctga aagtaaaaat ccgcatacat
     2701 cctcccctgc atatttcttc aacagcgggt tttgatataa tcgcctatct gttacagata
     2761 gttcaaacgg cattcaaacc cttacaaatg ccgtctgaaa tcatcggcat ccgtctatgc
     2821 aaaggatatt ttatgagttt acacagcgac atcctcgtcg tcggcgcagg cccggcgggt
     2881 ttaagttttg cagccgaact tgccggcagc ggtttgaaag ttaccctgat cgaacgcagc
     2941 ccactgactg ttctgcaaaa tcctccatat gacggacgcg aaatcgccct gacccatttt
     3001 tcccgcgaaa tcatgcagcg tttgggtatg tgggacaaaa ttcccgaaaa cgaaatctac
     3061 cctttgcgcg atgccaaagt gctgaacgga cgttccgact accagctcca ctttccccaa
     3121 ccgactgaag cgcgcggcga acctgccgac tgtttgggct atctgatttc caaccacaat
     3181 atccgccgcg ccgcctatga agtcgtatca caactcgaca atgtcagcat tctgaccgat
     3241 acggttgtca aagaagtcaa aacatctgac aacgaagcac aagtcatttt ggaaaacggc
     3301 aaaatcctga ctgcacgcct gcttttggcg gcagacagcc gcttctcgca aacccgtcga
     3361 caactcggta tctcctccga tatgcacgat tacagccgaa ccatgttcgt ctgccgtatg
     3421 aaacataccc tttccaacca acataccgct tacgaatgct tccattacgg acgcaccatc
     3481 gcactgctgc cgctggaaga gcacctgacc aataccgtca ttaccgtcga taccgataaa
     3541 atcaacagcg ttcaaaacct ttcgcccgaa gaattggcgg caagcgtaaa agaacaactc
     3601 aagggtaggc ttggcgacat ggaactggtc agcagcatcc accattaccc ccttgtcggt
     3661 atgattgcca aacgtttcta cggcaaacgc agcgcgctga tcggcgatgc ggctgtcggt
     3721 atgcaccccg ttaccgcaca cggtttcaac ttgggtctgt ccagcgcaga tattttggca
     3781 aaactgatac tcgaagccga gcagcgcggt caggacatcg gcgcatcttc cttgttggaa
     3841 aaatacagca acaaacatat gctgcacgcc catccgcttt atcacggaac caatatgatg
     3901 ctgaaactgt ttaccaacga aaccgcgccg gcaaaactgc tgcgcggttt ggtattgcgt
     3961 gccggcaaca acttcccgcc gctgaaaaag ctgattacca aacaactgac cggttgaaaa
     4021 ctgccgaaat gccgtctgaa acgtttcaga cggcattttt g
//
LOCUS       DAWHSK010000183         4052 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.183, whole genome shotgun sequence.
ACCESSION   DAWHSK010000183 DAWHSK010000000
VERSION     DAWHSK010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.183"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..819
                     /locus_tag="WIC03_001667"
     CDS             <1..819
                     /locus_tag="WIC03_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VENN motif pre-toxin domain-containing protein"
                     /protein_id="HEZ3884138.1"
                     /translation="ATANKGKCQDGAIGAAVGEIVGEALVKNTDFSDMTPEQLDLEVK
                     KITAYAKLAAGTVAGVTGGDVNTAAQTAQNAVENNAVKAVVTAAKVVYKVARKGLKNG
                     KINVRDLKQTLKDEGYNLADNLTTLFDETLDWNDAKAVIDIVVGTELNRANKGEAAQK
                     VKEVLEKNRPYIPNKGAVPNMSTYMKNNPFGKQLAQISEKTTLPTQQGQSVFLVKRNQ
                     GLLKTGDRFYLDGQHKNHLEVFDKNGNFKFVLNMDGSLNQMKTGAAKGRKLNLK"
     gene            828..1205
                     /locus_tag="WIC03_001668"
     CDS             828..1205
                     /locus_tag="WIC03_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884139.1"
                     /translation="METLNDIKKILINVGLYQGFDLTDPKVSEEVNHETANMKWIKDY
                     TSDGNWDNEFKEDLKNFLDYMEVCQLALNDKNFKIASNSLFMAMIYAGNLSLIFDSIK
                     TDISTLLSAEYKKNSFSWPSLDE"
     gene            1483..1857
                     /locus_tag="WIC03_001669"
     CDS             1483..1857
                     /locus_tag="WIC03_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884140.1"
                     /translation="MKNFNVVKESLRELGIKQGFDLYEKATTEKLNSEDPLDLQWLSN
                     YSSDWNDELEEDFDSFFQHMKEYQYAIDNEDIKSACSSLCEAMLYVGNIKNFFEFLKS
                     DMIRLLRGESKTTDFQWPQFDE"
     gene            1892..3697
                     /locus_tag="WIC03_001670"
     CDS             1892..3697
                     /locus_tag="WIC03_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HEZ3884141.1"
                     /translation="MRKVCASARTRSMIYGSMPSEKLTIFQTAFVMQVNIQIPYILPR
                     CVRAEDTPYACYLKQLQVTKDVNWNQVQLAYDKWDYKQEGLTGAGAAIIALAVTVVTA
                     GAGAGAALGLNGAAAAATDAAFASLASQASVSLINNKGNIGNTLKELGRSSTVKNLMV
                     AVATAGVADKIGASALNNVSDKQWINNLTVNLANAGSAALINTAVNGGSLKDNLEANI
                     LAALVNTAHGEAASKIKQLDQHYITHKIAHAIAGCAAAAANKGKCQDGAIGAAVGEIV
                     GEALVKNTDFSRMSATEIEKAKAKITAYSKLVAGTASAVVGGDVNTAANAATVAVENN
                     TLYPRCVGAKCDEFQKEQQKWIRENPEEYREVLLLQTGFIPIIGDIQSFVQAQTAADH
                     LFALLGVVPGIGESIQAYKVAKAAKNLQGMKKALDKAATVATAQGYVSKTKIKIGQTE
                     LRVTAATDKQLLKAIGEGRDTTGKMTEQLFDSLAKQNGFRVLSGGKYGGNNGFDHVWQ
                     AADGSVVLIVESKQIRNGTVQLNPNGAGGYTQMSREWIKQVVKSLPDGSPAKAVVLKA
                     NQNGKLKTAIAGVDRQTGKAVILSVKVPSKTNIRR"
     gene            3700..>4052
                     /locus_tag="WIC03_001671"
     CDS             3700..>4052
                     /locus_tag="WIC03_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma"
                     /protein_id="HEZ3884142.1"
                     /translation="MGHNMMTTQKWYEHITNVIIGNTANFNSGCPESIDYVDEKKGVP
                     LAAMKYILMYTEAAASHAYLFEHDLKKFKQYAYVAGKLGILQSVDDEDPEPFFFPCDM
                     LNIQDPMFLMLMSDSP"
BASE COUNT         1315 a          735 c          964 g         1038 t
ORIGIN      
        1 gcgacggcga ataagggtaa gtgtcaggac ggtgcgattg gcgcagccgt gggtgagatt
       61 gttggggaag ctttggttaa aaataccgat tttagcgata tgaccccgga acaattagat
      121 ctggaagtta agaaaattac cgcctatgcc aaacttgcgg caggtacagt tgcaggcgta
      181 acgggaggag atgtcaatac tgctgcacaa accgcacaaa acgcggtaga aaataatgcg
      241 gttaaagctg ttgtaactgc tgcaaaagtg gtttataagg tagccagaaa aggattaaaa
      301 aacgggaaaa tcaacgttag agatttaaaa cagacgttga aagacgaagg ttataattta
      361 gccgacaacc tgaccacctt attcgacgaa acattggatt ggaacgatgc caaagccgtt
      421 attgatattg tcgtcggaac agagctgaat cgcgctaata aaggggaagc ggcacaaaag
      481 gtcaaggaag ttttagaaaa aaatcgtcct tatatcccta ataaaggtgc tgtaccgaat
      541 atgagtacat acatgaaaaa taatcctttt ggaaaacagc tggctcaaat ttcagaaaag
      601 acaacgcttc cgacgcagca agggcagtct gtcttcttgg taaaaagaaa ccaagggtta
      661 ttaaaaaccg gtgataggtt ttatttagat ggccaacata aaaatcattt agaggttttt
      721 gataaaaatg ggaactttaa gtttgttcta aatatggatg gttcgcttaa ccaaatgaaa
      781 actggggcag caaaaggtcg taaattaaac ttaaaatagg aaattttatg gaaacattga
      841 atgatataaa aaaaatcttg attaatgtgg ggctttatca agggtttgat ttgacagatc
      901 caaaagtatc agaagaagtt aatcatgaaa cagctaatat gaaatggatt aaagattata
      961 cttcagacgg gaattgggat aatgaattta aggaggattt aaaaaacttt ttagattata
     1021 tggaagtatg ccaattagcc ctaaacgata aaaatttcaa aattgccagt aattctttat
     1081 ttatggctat gatttacgca ggtaatctat ctcttatatt tgattcaata aaaactgata
     1141 tatcaacatt attgagtgct gagtataaaa agaatagttt ttcatggcca tctcttgatg
     1201 aatagaaagc aagttgtagc ctgcatgaaa tctaaaaccc atgcataagg tgtgggcttc
     1261 agtatacgcg ttccatgatt tacggccata tgccgtctga aaagctcaat tttttcagac
     1321 ggcatttgtt atgaaagtaa atatttagat tccctgtata ctgtttagac tcgtgtgtgc
     1381 tgagtaagct gtagtctgca tgaaacctaa aactcgctca aaattaagct aagacattag
     1441 cagggcaagg gcgaaaattg aatcttaaat aaggtgattc agatgaaaaa ttttaatgta
     1501 gtaaaagaaa gtttaagaga gttaggaatt aaacaaggat ttgatcttta tgagaaagcc
     1561 acaactgaaa aattgaatag tgaagatcct cttgacttac aatggctttc taactattca
     1621 tctgattgga atgatgaatt agaagaagac tttgattctt tttttcagca tatgaaggaa
     1681 tatcaatatg ctattgacaa tgaagacatt aaatctgcat gtagttcact atgtgaagct
     1741 atgctctatg ttggtaatat taaaaatttt tttgagtttc tcaaaagcga tatgattaga
     1801 ctgttgagag gtgaaagtaa aacaacagac tttcaatggc cgcaatttga tgaatagcag
     1861 caagctgtag cctgcatgaa acctaaaatc catgcgtaag gtgtgtgctt cagcacgcac
     1921 gcgttccatg atttacggct caatgccgtc tgaaaagctc acaatttttc agacggcatt
     1981 tgttatgcaa gtaaatattc agattcccta tatactgccc agatgcgtgc gtgctgaaga
     2041 caccccctac gcttgctatt tgaaacagct ccaagtcacc aaagacgtca actggaacca
     2101 ggtacaactg gcgtacgaca aatgggacta taaacaggaa ggcttaaccg gagccggagc
     2161 agcgattatt gcgctggctg ttaccgtggt tactgcgggc gcgggagccg gagccgcact
     2221 gggcttaaac ggcgcggccg cagcggcaac cgatgccgca ttcgcctcgc tggccagcca
     2281 ggcttccgta tcgctcatca acaacaaagg caatatcggt aacaccctga aagagctggg
     2341 cagaagcagc acggtgaaaa atctgatggt tgccgtcgct accgcaggcg tagccgacaa
     2401 aatcggtgct tcggcactga acaatgtcag cgataagcag tggatcaaca acctgaccgt
     2461 caacctggcc aatgcgggca gtgccgcact gattaatacc gctgtcaacg gcggcagcct
     2521 gaaagacaat ctggaagcga atatccttgc ggctttggtg aatactgcgc atggagaagc
     2581 agccagtaaa atcaaacagt tggatcagca ctacattacc cacaagattg cccatgccat
     2641 agcgggctgt gcggcagcgg cggcgaataa gggcaagtgt caagatggcg cgataggcgc
     2701 tgcagtcggt gagattgttg gtgaggcttt ggttaagaat actgatttca gtcgtatgag
     2761 tgcgaccgaa atcgaaaaag ctaaagcgaa gattactgcc tattcaaaac tggttgccgg
     2821 cactgcgtct gccgttgtag gcggggatgt gaatacagcg gcgaatgcgg ctactgtggc
     2881 ggtggagaat aatactttgt atcctagatg cgttggtgca aagtgtgatg aatttcaaaa
     2941 ggaacaacaa aaatggatac gtgaaaatcc tgaagaatat cgagaagttt tgcttcttca
     3001 gacaggattt attccaatta tcggtgatat acagagtttt gtacaagcac agaccgctgc
     3061 cgatcacctg tttgctttgc tgggtgtggt tccgggtatc ggtgaatcga tacaggccta
     3121 taaagtagcg aaagcggcaa aaaatttaca aggcatgaaa aaagccttgg acaaggcagc
     3181 aaccgttgcc actgcacagg gctatgtcag taaaaccaaa atcaaaatcg gtcaaactga
     3241 attaagggtt actgcagcaa ctgacaaaca attgctgaaa gctattggcg aaggaaggga
     3301 cacgacaggt aaaatgaccg agcagttatt tgactcttta gctaaacaaa atggcttcag
     3361 agtgctttcg ggcggcaaat acggcggaaa taacggtttt gatcatgtat ggcaggctgc
     3421 cgatggtagt gttgttttga ttgtagaaag taagcagatt aggaacggta cggtacagct
     3481 gaatccgaat ggtgcgggtg gatatacgca gatgagtcgt gaatggatta aacaagttgt
     3541 aaaaagttta cctgatggta gtcctgctaa ggcagttgtc ttaaaagcaa atcagaacgg
     3601 caaattaaaa acggcaatag caggcgttga tcgtcaaaca ggtaaggccg ttattctttc
     3661 tgtcaaagtt ccttctaaaa ccaatataag gagataacaa tggggcacaa tatgatgacc
     3721 acccaaaaat ggtatgaaca tattactaat gtaatcatag gcaatactgc taatttcaat
     3781 agcggttgcc ccgaatctat agattatgta gatgaaaaaa aaggcgtgcc gcttgcagcg
     3841 atgaaataca ttttaatgta cactgaagct gcggcttccc atgcctatct atttgaacat
     3901 gatcttaaga aattcaagca atatgcttat gttgcaggaa agttgggtat tttgcagagt
     3961 gtagatgatg aagaccccga acccttcttc tttccctgcg acatgctcaa cattcaagat
     4021 ccgatgtttc tgatgctgat gagcgacagc cc
//
LOCUS       DAWHSK010000184         4045 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.184, whole genome shotgun sequence.
ACCESSION   DAWHSK010000184 DAWHSK010000000
VERSION     DAWHSK010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4045
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.184"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..805)
                     /locus_tag="WIC03_001672"
     CDS             complement(<1..805)
                     /locus_tag="WIC03_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEZ3884143.1"
                     /translation="MNTLYTLFATCPRGLETVLSQELESLGCTDVQVFDGGVSCRGGL
                     EQVYAANLHSRTASRILLRLTKGTYRNERNIYKLAKNINWFNWFTLQQTFKVKVEAKR
                     ANVKSIQFVGLTVKDAVCDAFRDIYDARPSVDKAAPDVRIHAFLDERNVEIFIDTSGE
                     ALFKRGYRLDTGEAPLRENLAAGLLLSAGYDGTQPFQDPFCGSGTIAIEAAWIAARRA
                     PGMMRRFGFEKLQNFDKTLWSDLRRRAEAQVKPAFAPISGSDSDRRIIQT"
     gene            complement(854..1276)
                     /locus_tag="WIC03_001673"
     CDS             complement(854..1276)
                     /locus_tag="WIC03_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884144.1"
                     /translation="MNKNRKLLLAALLLIAFAAVKLVLLQWWQAQQPQAVAAQCDLTE
                     GCTLPDGSRVRAAAVSTKKPFDIYIEHAPAGTEQVSISFSMKNMDMGFNRYMFERQPS
                     GTWQAVRIRLPICVEGRRDFTADITIGSRTFQTAFTAE"
     gene            complement(1269..2078)
                     /locus_tag="WIC03_001674"
     CDS             complement(1269..2078)
                     /locus_tag="WIC03_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEZ3884145.1"
                     /translation="MIQDTRPLIRVVAGILLDSDGNYLLSSRPEGKPYAGYWEFAGGK
                     VEAGETDFQALQREFEEELGIRILAATPWLTKIHSYEHARVCLKFLWVNPGQWTGEPQ
                     SREGQEWSWQKAGDFTVAPMLPANGALLRSLSVPRRLYGSLKTGLHGENSMGAYRVLP
                     LGSAEGSGANVLMEAAQWQDRPEHADSVWMMVQTREQWRQAQEKGADVVVWRVCNDVQ
                     AQEAAEALRQGVSVPLVLAANGQTVARYGKLWLGLGAHVVVRDETIGKNHE"
     gene            complement(2160..2537)
                     /locus_tag="WIC03_001675"
     CDS             complement(2160..2537)
                     /locus_tag="WIC03_001675"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEZ3884146.1"
                     /translation="MIYGIGTDIVSLKRIIRLNKKFGQAFAGRILTPEELLEFPQAGK
                     PVNYLAKRFAAKEAFAKAVGTGIRGAVSFRNIGIGHDALGKPEFFYGPALSKWLEEQG
                     ISRVSLSMSDEEDTVLAFVVAEK"
     gene            complement(2737..3504)
                     /locus_tag="WIC03_001676"
     CDS             complement(2737..3504)
                     /locus_tag="WIC03_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEZ3884147.1"
                     /translation="MQEIMQSIVFVAAAILHGITGMGFPMLGTTALAFIMPLSKVVAL
                     VALPSLLMSLLVLCSNNKKGFWQEIVYYLKTYKLLAIGSVVGSILGVKLLLILPVSWL
                     LLLMAIITLYYSVNGILNVCAKAKNIQVVANNKNMVLFGFLAGIIGGSTNAMSPILLI
                     FLLSETENKNRIVKSSNLCYLLAKIVQIYMLRDQYWLLNKSEYGLIFLLSVLSVIGLY
                     VGIRLRTKISPNFFKMLIFIVLLVLALKIGHSGLIKL"
     gene            complement(3514..>4045)
                     /locus_tag="WIC03_001677"
     CDS             complement(3514..>4045)
                     /locus_tag="WIC03_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEZ3884148.1"
                     /translation="DITFSEDVGECPLHSDSSFSENPESYLVMYVVKSANDGGNSLFL
                     SSSDIVNQLSKTETGKKHLKTLTGNLYPFKTPASFDKKQGVRWGNILSVNTQMIRFRS
                     DCIYKGIEENRNKVSKEMVLALDYLVNVIKNASDIQEFSAQDDGLIIIDNVNGLHART
                     DYTDKNRHYIRARITV"
BASE COUNT         1013 a          999 c          970 g         1063 t
ORIGIN      
        1 ccgtctgaat gatgcggcgg tcgctgtcgc tgcccgaaat cggtgcaaaa gccggtttaa
       61 cctgcgcttc ggcacggcgg cgcaaatccg accacagcgt tttatcgaaa ttttgcagtt
      121 tttcaaaacc gaaacggcgc atcatacccg gcgcgcggcg ggcggcaatc caagcggctt
      181 cgatagcaat cgtgccgctg ccgcaaaacg ggtcttgaaa cggctgcgtg ccgtcgtagc
      241 ctgccgagag cagcaatccg gcggcaaggt tttcgcgcag cggggcttcg ccggtatcga
      301 gacggtagcc gcgtttgaac agggcttcgc ccgaagtgtc gatgaagatt tcgacattgc
      361 gttcgtccaa aaaggcgtgg atgcgaacat cgggcgcggc tttgtccacg ctcggacgtg
      421 cgtcgtaaat gtcgcggaaa gcgtcgcaga cggcatcttt gacggtcagt ccgacaaatt
      481 ggatgctctt aacgttggca cgctttgcct cgactttgac tttgaacgtc tgctgtaaag
      541 taaaccaatt aaaccagttg atatttttgg cgagtttgta gatgttgcgc tcattgcggt
      601 atgtcccttt ggtcaggcgc agcaggatac ggctggcagt acgcgaatgc aggttggcgg
      661 cgtaaacctg ttccaatccg ccccggcagg aaacgccgcc gtcaaacact tgtacatcgg
      721 tacagccgag gctttcgagt tcttgagata aaacggtctc caagccgcgc gggcaggtgg
      781 cgaaaagtgt atataaagta ttcataacgt gtcctttccg gacggatgtt ccggcaatgg
      841 cggattgaaa ggtttattcg gcggtaaatg ccgtctgaaa tgtccgactg ccgattgtaa
      901 tgtctgccgt aaaatcgcgc ctgccttcga cacagatggg caggcggatg cgtactgcct
      961 gccaagtccc cgacggttgc cgctcgaaca tatagcggtt gaaacccata tccatatttt
     1021 tcatactgaa gctgatgctg acctgttccg tgccggcggg cgcgtgttcg atataaatat
     1081 caaacggttt tttggttgaa acggcggcgg cgcggacgcg gcttccgtcc ggcagcgtgc
     1141 aaccctcggt caaatcgcat tgcgccgcca cagcttgcgg ctgctgcgcc tgccaccatt
     1201 gcaacaaaac gagcttgacg gcggcaaagg caatcagcag cagtgcggca agcagtaatt
     1261 tacggttttt attcatgatt cttccctatt gtttcatccc ttaccaccac gtgcgccccc
     1321 aatccgagcc atagttttcc ataacgtgca accgtctgtc cgtttgctgc aagtacgagc
     1381 ggcacggata cgccctgccg cagggcttct gccgcctctt gtgcctgaac atcattgcac
     1441 acgcgccaca cgacaacatc cgcgcccttt tcctgcgcct gccgccattg ttcgcgggtc
     1501 tgcaccatca tccacacgct gtcggcgtgt tcgggtctgt cctgccattg cgccgcctcc
     1561 atcaaaacgt tcgcaccgct tccctctgcc gaacccaaag gcaggacgcg gtacgcgccc
     1621 atactgttct ccccgtgcaa acccgttttc aggctgccgt acaaacggcg cggaacggac
     1681 agcgaacgca aaagcgcgcc gttggcgggc agcatggggg caacggtaaa atcacccgcc
     1741 ttctgccaag accattcctg cccttcgcgg gattgcggtt cgcccgtcca ttggccgggg
     1801 ttgacccata ggaatttcag gcagacgcgg gcgtgttcgt aggaatggat tttggtcagc
     1861 caaggcgtgg cggcaaggat gcggatgccg agttcttctt caaactcgcg ttgcaaggct
     1921 tggaagtcgg tttcgcccgc ttcgaccttg ccgccggcaa attcccaata tccggcatag
     1981 ggtttgcctt cggggcgcga gctgagcagg tagttgccgt ctgaatcgag caggatgccg
     2041 gcaacgacgc ggataagggg tcgggtgtct tgaatcatgg cgggtacgcg gttggtcggt
     2101 cggagtgcaa atgagggacg agcaatgccg tcaacgggtt tgccgcattt cagacggcat
     2161 tatttttcgg caacgacaaa cgccaatacg gtgtcttctt cgtcgctcat gctgaggctg
     2221 acgcggctga tgccttgttc ctccagccat ttggacaggg cggggccgta gaaaaattcg
     2281 ggcttgccca atgcgtcatg cccgatgccg atgttgcgga aggaaaccgc gccgcgtatg
     2341 cccgtgccga cggctttggc gaaggcttct ttggcggcaa agcgtttggc gaggtagttg
     2401 acgggtttgc ccgcttgcgg aaattcaagc agctcttccg gagtgaggat gcgcccggca
     2461 aacgcctgtc cgaatttttt gtttaagcgg atgatgcgct tgagggaaac aatgtctgtg
     2521 ccgatgccgt aaatcatatt tgcgctcctt cgcccttggt tgcgggtagt gatgaatgtg
     2581 atgatgaatg ccgtcaattt cttggggcgt taaaatggcg tgaatatcca cttttggggt
     2641 tttgtcggta acaattttca ctgtaacctt ttgcatttga aattctaaaa catcgtgcca
     2701 attatttaat aaggagttaa ggcatttaat aatgaattaa agtttgatta aacccgaatg
     2761 cccgattttc agagccaata ccaataaaac aataaaaatt aacattttaa aaaaatttgg
     2821 gctaatctta gtccttaacc gaattccaac atacaatcca ataacagaca atacggacag
     2881 taaaaatatt aaaccgtatt cactcttatt taataaccaa tactggtctc ttagcatata
     2941 tatttgaaca attttcgcca aaagatagca tagattgctt gattttacga tacgattttt
     3001 attttctgtt tcgctaagca aaaatattaa caatatggga gacatggcat tggttgaacc
     3061 gccgatgatg cctgccaaaa acccaaaaag aaccatattc ttattattgg caactacttg
     3121 aatatttttt gcttttgcac atacatttaa aataccattg acagaataat acaatgtaat
     3181 gattgccatc agtaaaagca gccaagacac tggaagtatc aaaagcaact tcacccccaa
     3241 aatgctgcca acgacgctgc cgatagcaag caatttatag gtttttaaat aataaacaat
     3301 ctcttgccaa aaaccctttt tgttattgct gcatagaacc aacaagctca ttaacaggct
     3361 tggtaatgcc accaaggcaa caaccttaga caatggcatg ataaaagcca atgcggttgt
     3421 accgagcatc ggaaatccca tgcctgtaat tccgtgcagt attgcggcag caacaaaaac
     3481 gatagattgc attatttctt gcataaccgt cctttataca gtaattcttg ctctaatata
     3541 atgcctgttt ttatccgtat aatcagttct ggcatgcaag ccattgacat tgtcaataat
     3601 aatcaaacca tcatcttgtg cagaaaattc ttgaatatca ctcgcatttt ttataacatt
     3661 tacaagataa tcaagtgcaa gtaccatttc ctttgatact ttatttctat tttcttcaat
     3721 acctttatag atacaatcac ttctaaatct aatcatttga gtattgaccg ataagatatt
     3781 accccatctc acaccttgtt ttttatcaaa tgatgctggt gttttaaatg gatataaatt
     3841 gcccgttaat gtttttaagt gttttttacc ggtttctgtt ttagataact gattgacaat
     3901 atctgatgaa cttaaaaata gggaattacc tccatcattg gctgatttta ctacatacat
     3961 aaccaaataa ctttccgggt tttcactaaa agatgaatca ctatgaagtg gacattctcc
     4021 aacatcttcc gagaaagtta tatca
//
LOCUS       DAWHSK010000185         4002 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.185, whole genome shotgun sequence.
ACCESSION   DAWHSK010000185 DAWHSK010000000
VERSION     DAWHSK010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4002
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.185"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(67..1317)
                     /locus_tag="WIC03_001678"
     CDS             complement(67..1317)
                     /locus_tag="WIC03_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEZ3884149.1"
                     /translation="MFSKSVTLAQYDPDLAAAIAQEDQRQQDHVELIASENYVSCAVM
                     ETQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRVKKLFGAQYANVQPHSGSQAN
                     QAVYASVLKPGDTILGMSLAHGGHLTHGASVNISGKLYNAVTYGLDENEVLDYAEVER
                     LALEHKPKMIVAGASAYALQIDWAKFREIADKVGAYLFVDMAHYAGLIAGGEYPNPVP
                     FCDFVTTTTHKTLRGPRGGVILCRDNTHEKALNSSIFPSLQGGPLMHVIAAKAVAFKE
                     ALQPEFKQYAKQVKINAAAMAEELVKRGLRIVSGRTESHVFLVDLQPMKITGKAAEAA
                     LGKAHITVNKNAIPNDPEKPFVTSGIRIGSAAMTTRGFNEADARVLANLVADVLSNPE
                     DEANLAKVREQVTALCNKYPVYGA"
     gene            complement(1384..1579)
                     /locus_tag="WIC03_001679"
                     /pseudo
     CDS             complement(1384..1579)
                     /locus_tag="WIC03_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221594.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1798..3573)
                     /gene="ggt"
                     /locus_tag="WIC03_001680"
     CDS             complement(1798..3573)
                     /gene="ggt"
                     /locus_tag="WIC03_001680"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEZ3884150.1"
                     /translation="MAKTYLLTALIMSMTISGCQVIHANQGKVNTHSAVITGADAHTP
                     EHATGLIEQKQVIASDFMVASANPLATQAGYDILKQGGSAADAMVAVQTTLSLVEPQS
                     SGLGGGAFVLYWDNTAKTLTTFDGRETAPMRATPELFLDKDGQPLKFMEAVVGGRSVG
                     TPAIPKLMETIHQRYGVLPWGKLFDTPIRLAKQGFEVSPRLAISVEQNQQHLARYPKT
                     AAYFLPNGVPLQAGSLLKNLEFADSVQALAAQGAKALHTGKYAQNIVSVVQNAKDNPG
                     QLSLQDLSDYQVVERPPVCVTYRIYEVCGMGAPSSGGIAVGQILGILNEFSPNQVGYD
                     AEGLRLLGDASRLAFADRDVYLGDPDFVPVPIRQLISKDYLKHRSQLLEQSDKALPSV
                     SAGDFIHEWVSSQAIELPSTSHISIVDKAGNVLSMTTSIENAFGSTLMANGYLLNNEL
                     TDFSFEPIKQGKQVANRVEPSKRPRSSMAPTIVFKAGKPYMAIGSPGGSRIIGYVAKT
                     IVAHSDWNMDIQDAISAPNLLNRFGSYELETGTTALQWQQALNDLGYKTDVRELNSGV
                     QAIIIEPSRLVGGADPRREGRVMGD"
     gene            complement(3741..3977)
                     /locus_tag="WIC03_001681"
     CDS             complement(3741..3977)
                     /locus_tag="WIC03_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin"
                     /protein_id="HEZ3884151.1"
                     /translation="MWIFSACSRGADPIGRPFAFVNILENPEIRSTLPLIANRPTFPQ
                     LWVNGELIGGSDIILQMYQSGELKPLIEEHSPKV"
BASE COUNT         1001 a          952 c          940 g         1109 t
ORIGIN      
        1 tccgcctgaa acattatgag atttcaggcg gcattggatt gcttagcgga atatttttaa
       61 aaaggcttac gcgccgtaaa cggggtattt attgcacaaa gcagttactt gttcgcggac
      121 tttggcgagg ttggcttcgt cttcggggtt agacaatacg tcggcaacca agttcgccaa
      181 tacgcgcgcg tcggcttcgt taaaaccgcg tgtggtcatg gcggcggaac cgatgcggat
      241 gccggaggta acgaagggtt tttccggatc attcgggatg gcgtttttgt tgacagtgat
      301 gtgcgctttg cccaaagcag cttcggcggc tttgccggta attttcatcg gttgcaggtc
      361 aacgaggaaa acgtggcttt cggtgcggcc ggaaacgatg cgcaaaccgc gtttaaccaa
      421 ctcttccgcc atggcggctg cattgatttt cacttgtttt gcgtattgtt tgaactcggg
      481 ttgcaatgct tctttaaacg ccacggcttt ggcggcgata acgtgcatca gcgggccgcc
      541 ttgcaggctt gggaagatgg aagagttcaa cgctttttcg tgggtattgt cgcggcacaa
      601 aattacgccg ccgcgaggac cgcgcagggt tttgtgggtg gtggtggtaa cgaagtcgca
      661 gaatggcacc gggttgggat attcgccacc ggcaatcagt ccggcgtagt gcgccatgtc
      721 gacaaagagg tatgcgccga ctttatcggc gatttcgcgg aattttgccc agtcgatttg
      781 caacgcgtag gcagacgcac ccgccacaat cattttgggt ttgtgttcga gcgcgaggcg
      841 ttcgacttcg gcataatcga gcacttcgtt ttcatccaaa ccataagtaa cggcattgta
      901 gagtttgcct gagatattaa cgctcgcgcc gtgggtcagg tggccgccgt gcgccagaga
      961 catacccaaa atggtgtcgc ctggttttaa aacggaagcg tacacggctt ggttggcttg
     1021 cgagccggag tgcggttgga cgttggcgta ttgcgcgccg aacagttttt tgacgcggtc
     1081 aatcgccaat tgttcgacaa tatcgacgta ttcgcagccg ccgtagtagc gtttgccggg
     1141 gtagccttcg gcgtatttgt tggtcagctg ggaaccttgc gtttccatta cggcgcagct
     1201 gacgtagttt tcagaggcaa tcagctcgac gtggtcttgc tggcgttggt cttcttgggc
     1261 aatggctgct gccaaatcgg ggtcatattg tgcgagggta acgctttttg aaaacatgtt
     1321 ctcggctcct ttgtgtaatc agggtatcat gagtgttttt tgtataaaaa aatacttcaa
     1381 aacctaaggc agatagtcca taatgcgtaa attttctttg gcattatcag gtaatttatt
     1441 taacatgctg gtttttagcg tctcattacc tttatgttag tacaaggcta cccaaaggca
     1501 atacatcaaa tggtaaattt tcggcagttt gtgcaaaatc aatcaaataa ccagcaccaa
     1561 ccacatcttt atcagccata acccgctgat acaccacttg ccaagacaac tctcctgccc
     1621 caccatgtct taaaatcaga tgaatggctt gtaatgctgt gtttggcgtc tcacaaattg
     1681 cgaagatctg ctcagaagct tgcttgatgg cttgtatttt tgtttgatta ctcacaattt
     1741 ccccctattt taatattaac ttaatgcggt caaattcaca aaatacaagc ttcacctcta
     1801 atcacccatc actcgacctt ctcggcgtgg atcggcacca ccaaccagcc tgcttggctc
     1861 gataataatg gcttgaacac ctgaatttag ctcacgcacg tcagtcttat agcccaaatc
     1921 atttaatgct tgttgccact ggagggcggt tgtacccgtt tctagttcat agctaccaaa
     1981 gcgatttaat aaattgggtg cactgatggc atcttggata tccatattcc agtcgctatg
     2041 tgccacaatc gtcttagcga catagccaat gatacggcta ccacctgggg agccgattgc
     2101 catataaggc ttgcctgctt taaatacgat ggttggtgcc attgaggagc gtggtctctt
     2161 gctgggctcg acacgattgg cgacctgttt gccctgcttt attggctcaa aactaaagtc
     2221 tgtcagctca ttattcagca ggtagccatt tgccatcaaa gttgagccaa acgcattttc
     2281 aatggaagtc gtcattgata gcacattgcc cgccttatcc acaattgata tatgactggt
     2341 agaaggtaac tcaatcgctt gtgaggacac ccactcatga ataaaatcgc ctgcagatac
     2401 gctaggcaat gccttatccg actgctcaag cagctggctg cgatgtttta ggtagtcttt
     2461 agaaatcaac tggcgaatgg gtactggtac aaaatcaggg tcgcccaaat atacatcacg
     2521 atccgcaaac gcaagcctag aagcgtcgcc caagagacgt aaaccttcag catcataccc
     2581 cacctgattg ggtgaaaatt catttaaaat ccccaaaatt tgacccacag caatcccacc
     2641 tgagcttggt gcacccatac cgcatacttc ataaatacga taagtcacac aaacaggcgg
     2701 gcgttccacc acttgataat cagataaatc ttgtaaggat aattgaccgg ggttatcctt
     2761 agcattttgg acaactgaaa cgatattttg ggcatattta ccagtatgca gagcttttgc
     2821 accttgagct gctaacgcct gaacactgtc agcaaattct aaatttttca gcaagctgcc
     2881 tgcttgtagc ggcacaccat tcggcaaaaa ataagcggct gtttttggat agcgtgccaa
     2941 atgctgctga ttttgctcaa ccgagatggc aagccttggc gacacctcaa agccttgttt
     3001 tgccaagcgg atcggtgtat caaataattt tccccaaggc aatacaccgt atcgctgatg
     3061 tattgtctcc atcagtttag ggatagcagg cgtacccacc gagcgaccac cgaccaccgc
     3121 ttccataaat ttcaatggtt gaccatcttt atccaaaaat aattccggcg tcgcacgcat
     3181 cggtgccgtc tcacgcccat caaatgtggt caatgttttg gcggtattat cccaatacaa
     3241 cacaaatgca ccaccgccca agcctgacga ctgtggctct accaagctta gtgtcgtctg
     3301 caccgccacc atcgcatctg cagcgctacc gccttgcttt aagatatcat agccagcttg
     3361 tgttgctaat ggattggctg acgctaccat aaaatcactt gcaatcacct gcttttgttc
     3421 gatcagtccc gttgcatgtt caggcgtgtg agcgtctgca cctgtgatga cagcagaatg
     3481 agtattaacc ttaccttgat tggcatggat gacttgacat ccagagattg tcatagacat
     3541 tatcaatgca gtcaataaat atgttttagc cacaagcact ccttcgcctg agtttgattg
     3601 atgattcata caaggcatgt tgattattgt atttaatatg gctaaataat tcaatccaaa
     3661 ctatcaatct tgaccatcaa aaaaagaccg ctaatgtcat cagcagtctt ttttgatatt
     3721 tattttaaga tattaagtaa tcagaccttt gggctatgct cttcaatgag tggttttagc
     3781 tcacctgatt ggtacatttg taggataata tcactaccac cgattaactc accattaacc
     3841 caaagctgtg gaaaggttgg ccgattggcg atgagtggta gagtactgcg aatttctggg
     3901 ttttctagga tattaacaaa agcaaagggt ctgccaattg ggtcagcacc tctactgcac
     3961 gcgctgaaaa tccacattgg ggaaactggg gcgtgccttt ca
//
LOCUS       DAWHSK010000186         3989 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.186, whole genome shotgun sequence.
ACCESSION   DAWHSK010000186 DAWHSK010000000
VERSION     DAWHSK010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3989
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.186"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(95..991)
                     /gene="argB"
                     /locus_tag="WIC03_001682"
     CDS             complement(95..991)
                     /gene="argB"
                     /locus_tag="WIC03_001682"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEZ3884152.1"
                     /translation="MESENIISAADKARILAEALPYIRRFSGSVAVIKYGGNAMTEPA
                     LKEGFARDVVLLKLVGIHPVIVHGGGPQINAMLEKVGKKGEFVQGMRVTDKEAMDIVE
                     MVLGGHVNKEIVSMINTYGGHAVGVSGRDDHFIKAKKLLIDTPEQNGVDIGQVGTVES
                     IDTGLVKGLIERGCIPVVAPVGVGEKGEAFNINADLVAGKLAEELNAEKLLMMTNIAG
                     VMDKTGNLLTKLTPKRIDELIADGTLYGGMLPKIASAVEAAVNGVKATHIIDGRMPNA
                     LLLEIFTDAGIGSMILGGGEDA"
     gene            1161..2291
                     /locus_tag="WIC03_001683"
     CDS             1161..2291
                     /locus_tag="WIC03_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase family protein"
                     /protein_id="HEZ3884153.1"
                     /translation="MIKISTRFDAGSVVVKDLTDPSNIRLALRPDNASDFAQWFYFRL
                     QGAAYQNCIMHFDNAAEAAYPKGWEGYQACASYDRRNWFRVPTSYENGVLTVNHTPLS
                     NSVYYAYFEPYSEEQHLNLLGDAQGSGLCRIDDLGSTVQGRDINLLTIGNQVESDLKI
                     WITARQHPGETMAEWFVEGLLGRLLDHQDATARTLLDRATFYIVPNMNPDGSALGNLR
                     TNAAGANLNREWENPTLEKSPEVFFVREKMLETGVDLFLDIHGDEGLPFIFVAGTEGV
                     PNYNPRISALETQFKTALLNASPDFQDEYGYEKDAPGQANMTLATNWVGNRFNCLAYT
                     LEMPFKDNANLPDDDFGWNGQRSLRLGEAALSAILNIIGDLR"
     gene            2362..3213
                     /locus_tag="WIC03_001684"
     CDS             2362..3213
                     /locus_tag="WIC03_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEZ3884154.1"
                     /translation="MIIHPQFDPVLISIGPLAVRWYALSYILGFILFTFLGRRRIAQG
                     LSVFTKESLDDFLTWGILGVIFGGRLGYVLFYKFSDYLAHPLDIFKVWEGGMSFHGGF
                     LGVVIAIWLFGRKHGIGFLKLMDTVAPLVPLGLASGRIGNFINGELWGRVTDINAFWA
                     MGFPQARYEDAEAAAHNPLWSGWLQQYSMLPRHPSQLYQFALEGICLFAVVWLFSKKQ
                     RPTGQVASLFLGGYGIFRFIAEFARQPDDYLGLLTLGLSMGQWLSVPMIVLGIVGFVR
                     FGMKKQH"
     gene            complement(3296..3958)
                     /locus_tag="WIC03_001685"
     CDS             complement(3296..3958)
                     /locus_tag="WIC03_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecA family protein"
                     /protein_id="HEZ3884155.1"
                     /translation="MDSQKFTEASKRRLSELLDAKSEQGNTMRCDEVQGFMTALLSGP
                     DKLTPLDWLPEVLGDESQFTAAERSEIERLVLAMAMETTAAMSDKKLPDLWLYENEDG
                     GSDFYTWCNAYLYGLDIVPTDWFEAVDDEAFEELFYPIMALGGIYDEEDNGTIRLQFT
                     EGELAELESELPYALADIYRYWQAVINKPQTVRREGEKTGRNDPCPCGSGRKYKACCG
                     KN"
BASE COUNT          875 a         1168 c          971 g          975 t
ORIGIN      
        1 ttcaggaatg acggtttgga aattgcccga aaccccaaaa acagaaaccg gacaaacggg
       61 ttttccgcca aagccggcat tttccgactt tgcttcaggc atcttcccca ccgcccaaaa
      121 tcatcgaacc gataccggca tcggtaaaga tttccagcaa aagcgcgttg ggcatcctgc
      181 cgtcgatgat gtgcgtggct ttcacgccgt tgacggcggc ttcgaccgcg gaagcgattt
      241 tcggcagcat accgccatac agcgtgccgt cggcaatcag ttcatcaatc cgtttcggcg
      301 tgagtttggt cagcagattg cccgttttgt ccatcacacc ggcgatattc gtcatcatca
      361 agagtttttc agcgttcaat tcttccgcca atttgcctgc taccaaatcg gcgttgatgt
      421 tgaacgcttc gcctttttca cctacgccga cgggggcgac gacgggaatg cagccacgtt
      481 ctatcagccc tttaaccaaa ccggtatcga tgctttccac cgtaccgacc tgtccgatgt
      541 ccacgccatt ctgttcgggc gtatcgatca aaagtttctt cgccttaatg aaatggtcgt
      601 cgcgtccgct tacgccgacc gcgtgtccgc catatgtgtt aatcatcgac acgatttctt
      661 tattgacatg cccgcccaac accatttcga caatatccat cgcctctttg tcggtaacgc
      721 gcattccttg gacaaactca ccctttttgc cgactttttc aagcatcgca ttgatctgcg
      781 gcccgccgcc gtgaacgatg acgggatgaa tgccgaccag cttcagcagc acgacatcgc
      841 gggcaaaccc ttctttcaag gcaggttcgg tcatcgcgtt gccgccgtat ttgatgacgg
      901 cgaccgaacc ggaaaaccgg cggatgtaag gcagcgcttc ggcaaggata cgcgccttgt
      961 cggcggcgga aataatgttt tcagactcca taacggctcc ctattgttga caatttcggc
     1021 ttatgttaac gccaaacgcc gtctgaaaca atcttcagac ggcatccggc agactttcgg
     1081 gcaggttgaa gccttgcgat acgggcggta aaatcgcggt ttatccggtt cgagaaaccg
     1141 gcgacagcaa ggactttccc atgattaaaa tcagcacccg cttcgatgcc ggctcggtcg
     1201 tcgtcaaaga cctgaccgat ccttccaaca tccgcctcgc cctgcgtccc gacaacgcct
     1261 ccgattttgc acaatggttc tacttccggc tgcaaggcgc ggcatatcaa aactgcatca
     1321 tgcattttga caatgcggca gaagccgcct acccgaaagg ctgggaaggc tatcaggcgt
     1381 gtgcctcata cgaccgccgc aactggttcc gcgtaccgac ttcctacgaa aacggcgtac
     1441 tgaccgtcaa tcatacgccg ctgtccaaca gcgtgtatta cgcctatttc gaaccttact
     1501 ccgaagaaca gcatttaaac ctcctcggcg acgcgcaagg cagcggcctg tgccgcatcg
     1561 acgacttggg cagcaccgtg caagggcgcg acatcaatct gctgaccata ggcaatcagg
     1621 tcgaaagcga tttgaaaatc tggattaccg cccgccagca tccaggcgaa accatggcgg
     1681 aatggtttgt cgaaggactg ctcggcaggc tgctcgacca tcaggatgca accgcgcgca
     1741 ccctgctcga tcgcgccacg ttctatatcg tccccaacat gaaccccgac ggttcggcac
     1801 tgggcaacct tcgcaccaac gccgccggtg cgaacctcaa ccgcgaatgg gaaaatccga
     1861 ctttggaaaa aagccccgaa gtgttcttcg tgcgcgaaaa aatgctggaa accggcgtgg
     1921 atttgttctt ggacatccac ggcgacgaag ggctgccctt tatctttgtt gcaggaactg
     1981 aaggcgtgcc aaactacaat ccgcgcattt ccgcgttgga aacacagttc aaaaccgccc
     2041 ttttgaacgc cagccccgat ttccaagacg aatacggtta cgaaaaagat gcgcccggtc
     2101 aggcaaacat gaccttggca acaaactggg tcggcaaccg tttcaactgc cttgcctata
     2161 cgttggaaat gccctttaaa gacaatgcca acctgcccga cgacgatttc ggatggaacg
     2221 gtcaacggtc gttgcgcttg ggcgaagccg cgttgtccgc catcctcaac atcatcggcg
     2281 atttgcgctg aaacccttat tgcagaaatg ccgtctgaag catcatgcct ttcagacggc
     2341 atttgaaaac gaaagcctgt tatgattatc catccccaat tcgatcccgt cctcatcagt
     2401 atcggcccgc ttgccgtccg ctggtatgcc ctaagctaca tcctcggatt tattcttttt
     2461 acctttctcg gcagaaggcg catcgcgcaa ggcttgtccg tttttaccaa agaatcgctt
     2521 gacgacttcc tcacttgggg cattttgggc gtaatttttg gtgggcgttt gggttacgtc
     2581 ctgttttaca agttttccga ctacctcgcc catccgcttg atattttcaa ggtatgggaa
     2641 ggcggaatgt cgttccacgg cggctttttg ggtgtagtta ttgccatatg gttgttcggc
     2701 cgcaaacacg gcatcggctt cctcaaactg atggacacgg tcgcaccgct cgttccgctg
     2761 ggtctcgctt cgggacgtat cggcaacttc atcaacggcg aactttgggg acgcgttacc
     2821 gacatcaacg cattttgggc aatgggcttc ccgcaggcgc gttacgaaga tgccgaagcc
     2881 gccgcgcaca atcccctttg gtcaggttgg ttgcaacaat atagtatgct gccccgccat
     2941 ccctcgcagc tttatcagtt tgcacttgaa ggcatctgcc tgttcgccgt cgtttggctg
     3001 ttctctaaaa aacagcggcc gaccggacaa gtcgcctcac tcttcctcgg cggctacggc
     3061 atattccgct tcattgccga atttgcacgc caacccgacg actatctcgg gctgctgacc
     3121 ttggggctgt cgatggggca atggttgagc gtcccgatga ttgttttggg tatcgtcggc
     3181 tttgtccggt tcggcatgaa aaaacagcac tgaccgcctg tgaaaaatac cgtctgaaac
     3241 atggaaatcc ggtttcagac ggcattttta cgtttggttc atggaaacaa acgcttcaat
     3301 tcttaccgca acacgccttg tattttctgc cgctgccgca cggacaggga tcgttcctgc
     3361 ctgttttttc gccttccctg cggacggttt gcggcttgtt gatgaccgcc tgccagtagc
     3421 ggtagatatc cgccaatgca taaggcaact cggattccag ttccgccagc tcgccttctg
     3481 tgaattgcag acggatagtg ccgttgtcct cttcgtcgta aataccgccc aatgccatga
     3541 tgggataaaa caactcttca aacgcttcat catcgacggc ttcaaaccaa tcggtcggca
     3601 caatatccaa accgtaaaga taagcattgc accatgtgta aaaatcgctg ccgccgtctt
     3661 cgttttcata cagccacaaa tcgggcagtt ttttatccga catcgcggcg gttgtttcca
     3721 tcgccattgc caaaaccagc cgttcgattt cggaacgttc ggcggcggta aattgcgatt
     3781 cgtcgcccaa cacttcgggc agccagtcga gcggtgtcaa tttgtccggc ccgctcaaca
     3841 gcgccgtcat aaaaccttga acctcgtcgc aacgcatcgt gttgccttgt tcgcttttgg
     3901 catccaacaa ttcgctcaac cgccgtttgg atgcttcggt aaatttttgg gaatccatca
     3961 ttttcctttt caaatgggtt ttgcgccct
//
LOCUS       DAWHSK010000187         3964 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.187, whole genome shotgun sequence.
ACCESSION   DAWHSK010000187 DAWHSK010000000
VERSION     DAWHSK010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3964
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.187"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..375)
                     /locus_tag="WIC03_001686"
     CDS             complement(<1..375)
                     /locus_tag="WIC03_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEZ3884156.1"
                     /translation="MWSTRFWRKWRVWKKLFSLYREDSLISRLNRDGYLTSPPADFLE
                     LLSLCRDIHALTGGAFDPTVQALWKLYADYFAARPDAETPPPENSVKEALKRVGFGKV
                     SFDDREIRFAEKGMGLSLNGIAQ"
     gene            complement(311..594)
                     /locus_tag="WIC03_001687"
                     /pseudo
     CDS             complement(311..594)
                     /locus_tag="WIC03_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234221.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nosX protein required for nitrous oxide
                     reduction"
     gene            complement(603..1397)
                     /locus_tag="WIC03_001688"
     CDS             complement(603..1397)
                     /locus_tag="WIC03_001688"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEZ3884157.1"
                     /translation="MKAYLDLMRHVLDNGTDKSDRTGTGTRSVFGYQMRFDLGKGFPL
                     LTTKKLHLRSIIHELLWFLKGDTNIKYLKDNNVSIWDEWADENGDLGPVYGYQWRNWP
                     APDGRHIDQIANVLEQIKKNPDSRRLIVSAWNPALVDEMALPPCHALFQFYVADGKLS
                     CQLYQRSADIFLGVPFNIASYALLTMMMAQVCGLEAGEFVHTFGDAHLYRNHFEQAAL
                     QLEREPRALPVMKINPEVKDLFSFKFEDFELEGYDPHPHIKAAVSV"
     gene            1819..3153
                     /gene="gdhA"
                     /locus_tag="WIC03_001689"
     CDS             1819..3153
                     /gene="gdhA"
                     /locus_tag="WIC03_001689"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEZ3884158.1"
                     /translation="MTDLNTLFANLKQRNPNQEPFHQAVEEVFMSLDPFLAKNPKYTQ
                     QSLLERIVEPERVVMFRVTWQDDKGQVQVNRGYRVQMSSAIGPYKGGLRFHPTVDLGV
                     LKFLAFEQVFKNALTTLPMGGGKGGSDFDPKGKSDAEVMRFCQAFMTELYRHIGADTD
                     VPAGDIGVGGREIGYLFGQYKKIRNEFSSVLTGKGLEWGGSLIRPEATGYGCVYFAQA
                     MLQTRNDSFEGKRVLISGSGNVAQYAAEKAIQLGAKVLTVSDSNGFVLFPDSGMTEAQ
                     LAALIELKEVRRERVATYAKEQGLQYFENQKPWGVAAEIALPCATQNELDEEAAKTLL
                     ANGCYVVAEGANMPSTLGAVEQFIKAGILYAPGKASNAGGVATSGLEMSQNAIRLSWT
                     REEVDQRLFGIMQSIHESCLKYGKVGDTVNYVNGANIAGFVKVADAMLAQGF"
     gene            3295..3669
                     /locus_tag="WIC03_001690"
     CDS             3295..3669
                     /locus_tag="WIC03_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884159.1"
                     /translation="MIMDQYYMELKNKLSNRPILLDNTNDFLFVLVNTVKAMIENTDK
                     SQLSELDKILDGVTSQELKLAYDFCQGKFGQAGFSYRRHPNYFYLSSLIATFPEFELS
                     KADRDYLKGIINFDNYLLYELD"
     gene            3713..>3964
                     /locus_tag="WIC03_001691"
     CDS             3713..>3964
                     /locus_tag="WIC03_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone acetyltransferase"
                     /protein_id="HEZ3884160.1"
                     /translation="MIELKLVDESSFQAVLDLKISEADERARFVAPNVRSLADAWLYR
                     ENEDVFPRAIYWDKQVVGFLLLEIDKDEAEYFIWRIMIGQ"
BASE COUNT         1080 a         1039 c          931 g          914 t
ORIGIN      
        1 ttgcgcgatg ccgttcaagg acaaacccat acccttttcg gcaaaacgga tttcacggtc
       61 gtcaaacgag actttgccga agccgacccg tttcagggct tccttcacgc tattctccgg
      121 cggcggcgtt tccgcatcgg gacgggcggc aaaataatcg gcatacagtt tccacaacgc
      181 ctgcactgtc ggatcgaacg cgcctcccgt cagcgcgtga atatcgcggc acaggctcaa
      241 cagttccaaa aaatccgccg gcggcgaagt caaataaccg tcacggttca gacggctgat
      301 caggctgtct tcacggtaaa ggctgaacaa ttttttccaa acgcgccact tccgccaaaa
      361 ccttgttgac caaatccgcc gcctgtctgt tatccacgcc gaacaggcgc agctccgcac
      421 cggaacccag cgcgacacct ttccagagaa aaacacattt tcattgcgtt tttcatcccc
      481 gttgcgtttt tcatcatcgg cggcaaaagg attcggcagg aaagaaaccg ccgcgcccgc
      541 cgccgcaacg gcggcaaccg tcagaaaacg cctgcgcccg aaatgcctgc ccataccgcc
      601 tcctaaaccg acactgccgc cttgatatgc ggatgagggt cgtaaccttc caactcgaaa
      661 tcttcaaact tgaaggaaaa caaatctttg acttcaggat tgattttcat cactggcaag
      721 gcgcgcggtt cgcgttccaa ctgcaatgcg gcctgctcga aatggttgcg gtacaaatgc
      781 gcgtcgccaa acgtatggac aaactcgccc gcctccaatc cgcacacttg cgccatcatc
      841 atggtcaaca atgcgtagct ggcaatatta aacggcacac caaggaaaat atctgcacta
      901 cgctggtaaa gctggcagga cagtttgccg tcggcaacgt aaaactgaaa cagcgcgtgg
      961 cagggcggca aggccatttc atcgaccaaa gccggattcc acgccgatac aatcaggcgg
     1021 cgcgagtcgg gattcttctt gatttgttcc agcacattgg cgatttggtc gatatgcctg
     1081 ccgtcgggcg cgggccagtt acgccactgg tagccgtaaa ccgggcctaa gtcgccgttt
     1141 tcgtccgccc actcgtccca aatggaaaca ttgttgtcct tcaggtattt gatattggta
     1201 tcgcctttga gaaaccaaag cagctcgtgg ataatcgagc gcaaatgcag ctttttggtc
     1261 gtcagcagcg gaaaaccttt gcccaagtca aaacgcatct gataaccgaa tacggagcgc
     1321 gtacccgtac cggtgcggtc tgatttgtcc gtaccgttgt cgaggacgtg gcgcatcaag
     1381 tccaaatagg ccttcatcat catctttcat caaattaaac ggcgcatatt gtaacatttc
     1441 cggccaaagc ccgagtccgg acaaacgcac acgccgcccc cccggacgat aaaacgcaga
     1501 tacgggcagg caggattgtt ggcaatttca gtccttttcc acagtaaaac ccagtgggaa
     1561 aacaaaatta ccttgattgg aatcaaaaaa tctagtttaa ttacttagaa taaaatttca
     1621 ataatatttt tatttacgaa attaatttat gattattgat taattatcgg caacaaacat
     1681 caaacatcga aaatatggaa aaaataatgt caacaatttt tgccaaatcg ggcttggcat
     1741 cagaaaaaaa taggtttata ttcccaccta caaatttgtt ttcccattag tacactatca
     1801 accaaaagga gtatccgaat gactgacctg aacaccctgt ttgccaacct caaacaacgc
     1861 aatcccaatc aggagccgtt ccatcaggcg gttgaagaag tcttcatgag tctcgatccg
     1921 tttttggcaa aaaatccgaa atacacccag caaagcctgc tggaacgcat cgtcgaaccc
     1981 gaacgcgtcg tgatgttccg cgtaacctgg caggacgata aagggcaagt ccaagtcaac
     2041 cggggctacc gcgtgcaaat gagttccgcc atcggtcctt acaaaggcgg cctgcgcttc
     2101 catccgaccg tcgatttggg cgtattgaaa ttcctcgctt ttgaacaagt gttcaaaaac
     2161 gccttgacca ccctgcctat gggcggcggc aaaggcggtt ccgacttcga ccccaaaggc
     2221 aaatccgatg ccgaagtaat gcgcttctgc caagccttta tgaccgaact ctaccgccac
     2281 atcggcgcgg acaccgatgt tccggccggc gacatcggcg taggcgggcg cgaaatcggc
     2341 tacctgttcg gacaatacaa aaaaatccgc aacgagtttt cttccgtcct gaccggcaaa
     2401 ggtttggaat ggggcggcag cctcatccgt cccgaagcga ccggctacgg ctgcgtctat
     2461 ttcgcccaag cgatgctgca aacccgcaac gatagttttg aaggcaaacg cgtcctgatt
     2521 tccggttccg gcaacgtggc gcaatacgcc gccgaaaaag ccatccaact gggcgcgaaa
     2581 gtgctgaccg tttccgactc caacggcttc gtcctcttcc ccgacagcgg tatgaccgaa
     2641 gcgcaactcg ccgccttgat cgaattgaaa gaagtccgcc gcgaacgcgt tgccacctac
     2701 gccaaagagc aaggtctgca atactttgaa aaccaaaaac cgtggggcgt cgccgccgaa
     2761 atcgccctgc cctgcgcgac ccagaacgaa ttggacgaag aagccgccaa aaccctgttg
     2821 gcaaacggct gctacgtcgt tgccgaaggt gcgaatatgc cgtcgacttt gggcgcggtc
     2881 gagcaattta tcaaagccgg catcctctac gccccgggaa aagcctccaa tgccggcggc
     2941 gtagcaactt caggtttgga aatgagccaa aacgccatcc gcctgtcttg gactcgtgaa
     3001 gaagtcgacc aacgcctgtt cggcatcatg caaagcatcc acgaatcctg tctgaaatac
     3061 ggcaaagtcg gcgacacagt aaactacgtc aatggtgcga acattgccgg tttcgtcaaa
     3121 gttgccgatg cgatgctggc gcaaggcttc taagcaaacg ccgccgctcc gcaaacaaaa
     3181 tgccgtccga accgcaaatg ctgttcagac ggcatttcat tatccgcccg ttcgaatcgg
     3241 gtgatactat ttgtatccag aattgttgga tagtttggga gtggagagga agaaatgatt
     3301 atggaccaat actatatgga gttaaagaat aagttatcta acagaccaat cctactggat
     3361 aatactaatg attttctttt tgttttagta aatacagtga aagctatgat agaaaatact
     3421 gataaaagtc agctttcaga gttagataaa attttagacg gtgtcacaag tcaagaattg
     3481 aaacttgcct atgatttttg tcaagggaaa ttcggtcaag caggtttttc ttatcgtagg
     3541 cacccgaatt atttttattt gtccagttta attgcgactt ttccagaatt tgagttgtct
     3601 aaagcagacc gagattacct caaaggaatc ataaattttg ataactacct tttatatgag
     3661 ttagattagt ctttaatcac ctcaaaaccc tatttattca aaaggagaaa aaatgattga
     3721 gctgaaatta gtagatgaga gcagttttca ggcagtgctg gatttgaaaa tatctgaagc
     3781 tgatgaacga gcacgttttg tggctccaaa tgtacgctct ttagctgacg catggctcta
     3841 ccgggaaaat gaagatgtgt ttccgagggc aatctattgg gataagcaag tggttggctt
     3901 tctcctgctg gaaatagaca aggatgaagc agaatacttt atctggcgga taatgattgg
     3961 tcag
//
LOCUS       DAWHSK010000188         3932 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.188, whole genome shotgun sequence.
ACCESSION   DAWHSK010000188 DAWHSK010000000
VERSION     DAWHSK010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3932
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.188"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..1602
                     /locus_tag="WIC03_001692"
     CDS             <1..1602
                     /locus_tag="WIC03_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEZ3884161.1"
                     /translation="ASGKIEKSINPHKLDQPFPGKARWEKIQRAGSVRDLESIGGVLY
                     AQGWLSAGTHNGRDYALFFRQPVPKGVAEDAVLIEKARAKYAELSYSKKGLQTFFLAT
                     LLIASLLSIFLALVMALYFARRFVEPVLSLAEGAKAVAQGDFSQTRPVLRNDEFGRLT
                     KLFNHMTEQLSIAKEADERNRRREEAARHYLECVLEGLTTGVVVFDEQGCLKTFNKAA
                     EQILGMPLTPLWGSSRHGWHGVSAQQSLLAEVFAAIGAAAGTDKPVHVKYAAPDDAKI
                     LLGKATVLPEDNGNGVVMVIDDITVLIHAQKEAAWGEVAKRLAHEIRNPLTPIQLSAE
                     RLAWKLGGKLDEQDAQILTRSTDTIVKQVAALKEMVEAFRNYARSPSLKLENQDLNAL
                     IGDVLALYEAGPCRFAAELAGEPLMVAADTTAMRQVLHNIFKNAAEAAEEADVPEVRV
                     KSEVGRDGRIVLTVCDNGKGFGREMLHNAFEPYVTDKPAGTGLGLPVVKKIIEEHGGR
                     ISLSNQDAGGACVRIILPKTVKTYA"
     gene            1595..2872
                     /locus_tag="WIC03_001693"
     CDS             1595..2872
                     /locus_tag="WIC03_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HEZ3884162.1"
                     /translation="MRSSDILIVDDEIGIRDLLSEILQDEGYSVALAENAEEARKLRH
                     QARPAMVLLDIWMPDCDGITLLKEWAKNGQLNMPVVMMSGHASIDTAVEATKIGALDF
                     LEKPISLQKLLSAVENALKYGAAQTETGPVFDKLGNSAAIQEMNREVEAAAKRTSPVF
                     LTGEVGSPFETVARYFHKNGTPWVSPARVEYLIDMPTELLQKAEGGVLYVGDIAQYSR
                     NIQTGIAFIIGKAERCRVRVVASGSRAAGSDGIACEEKLAELLSESVVRIPPLRMQHE
                     DIPFLIQGIACNVAESQKIAPASFSEEALAALTRYDWPGNFDQLQSVVATLLLEADGQ
                     EIGAGAVSSLLGQNVPAEGAEDMVGGFNFNLPLRELREEVERRYFEYHIAQEGQNMSK
                     VAQKVGLERTHLYRKLKQLGIGVSRRAGEKTEE"
     gene            2963..>3932
                     /gene="dprA"
                     /locus_tag="WIC03_001694"
     CDS             2963..>3932
                     /gene="dprA"
                     /locus_tag="WIC03_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEZ3884163.1"
                     /translation="MTEDERFAWLQLAFTPYIGAESFLLLLRRFGSASNALSAPAEQV
                     APAVRHKQAAEAWRNAEKRASARQAAEAALEWEMRDGCRLMLLQDEDFPEMLTQGLTA
                     PPVLFLRGNVRLLHKPSAAIVGSRHATPQAMRIAKDFGKSLGGQNIPVVSGMASGIDT
                     AAHQGALEAEGGTIAVWGTGIDRIYPPSNKNLAYEIAEKGLIVSEFPIGTRPYAGNFP
                     RRNRLIAALSQVTLVVEAALESGSLITAGLAAEMGREVMAVPGSIDNPHSKGCHKLIK
                     DGAKLVECLDDILNECPGLLQNTGASSYSINKDTPDTGSRAVQTAYA"
BASE COUNT          896 a         1022 c         1241 g          773 t
ORIGIN      
        1 gcaagcggca aaatcgaaaa aagcatcaac ccgcacaagc tcgatcagcc gtttccaggt
       61 aaggcgcgtt gggaaaaaat ccaacgggcg ggttcggtca gggatttgga aagcataggc
      121 ggcgtattgt acgcgcaggg ctggctgtcg gcgggtacgc acaacgggcg cgattacgcc
      181 ttgtttttcc gtcagccggt tcccaaaggc gtggcagagg atgccgtctt aatcgaaaag
      241 gcaagggcga aatatgctga gttgagttac agcaaaaaag gtttgcagac ctttttcctg
      301 gcaaccctgc tgattgcctc gctgctgtcg atttttcttg cactggtcat ggcactgtat
      361 ttcgcccgcc gtttcgtcga acccgtccta tcgcttgccg agggggcgaa ggcggtggcg
      421 caaggcgatt tcagccaaac ccgtcctgtt ttacgcaatg acgagttcgg gcgtttgacc
      481 aagttgttca accacatgac cgagcagctt tccatcgcca aagaagcaga cgagcgcaac
      541 cgccggcgcg aggaagccgc cagacattat cttgaatgcg tgttggaggg gctgaccacg
      601 ggcgtggtgg tgtttgacga acaaggctgt ctgaaaacct tcaacaaagc ggcggaacag
      661 attttgggga tgccgcttac ccccctgtgg ggcagcagcc ggcacggttg gcacggcgtt
      721 tcggcgcagc agtccctgct tgccgaagtg tttgccgcca tcggcgcagc ggcaggtacg
      781 gacaaaccgg tccatgtgaa atatgccgcg ccggacgatg ccaaaatcct gctgggcaag
      841 gcaaccgtcc tgcccgaaga caacggcaac ggcgtggtaa tggtgattga cgacatcacc
      901 gttttgatac acgcgcaaaa agaagccgcg tggggcgaag tggcaaaacg gctggcacac
      961 gaaatccgca atccgctcac gcccatccag ctttccgccg aacgtctggc gtggaaattg
     1021 ggcgggaagc tggatgagca ggatgcacaa atcctgacgc gttcgaccga caccatcgtc
     1081 aaacaggtgg cggcattgaa ggaaatggtc gaagcattcc gcaattatgc gcgttcccct
     1141 tcgctcaaat tggaaaatca ggatttgaac gccttaatcg gcgatgtgtt ggcattgtat
     1201 gaagccggtc cgtgccggtt tgcggcggag cttgccggcg aaccgctgat ggtggcggcg
     1261 gatacgaccg ccatgcggca ggtgctgcac aatattttca aaaatgccgc cgaagcggcg
     1321 gaagaagccg atgtacccga agtcagggta aaatcggaag tggggcggga cggtcggatt
     1381 gtcctgacgg tttgcgacaa cggcaaaggg ttcggcaggg aaatgctgca caacgccttc
     1441 gagccgtatg taacggacaa accggcggga acgggattgg gtctgcccgt ggtgaaaaaa
     1501 atcattgaag aacacggcgg ccgcatcagc ctgagcaatc aggatgcggg tggggcgtgt
     1561 gtcagaatca tcttgccaaa aacggtaaaa acttatgcgt agcagcgata ttttaattgt
     1621 agacgacgaa atcggcatcc gcgacctgct gtcggaaatc ctgcaggacg aaggttattc
     1681 ggtcgcattg gcggaaaacg ccgaagaggc gcgcaagctg cgccatcagg cgcgtccggc
     1741 gatggtgctg ctggatattt ggatgcccga ttgcgacggc atcacccttt tgaaggagtg
     1801 ggcgaaaaac gggcagctta atatgccggt cgtcatgatg agcgggcatg ccagcattga
     1861 cacagcagtg gaagccacca aaatcggtgc gctcgacttt ttggaaaaac cgatctccct
     1921 gcaaaaactg ctgtctgccg tcgaaaacgc gttgaagtac ggtgcggcgc aaaccgaaac
     1981 ggggcctgta ttcgacaagc tgggcaacag tgcggcgatt caggagatga accgcgaagt
     2041 cgaagcggcg gcaaagcgca cgtcccctgt atttttgacg ggtgaggtgg gttcgccgtt
     2101 tgaaaccgta gcgcgctatt tccataaaaa cggtacgccg tgggtcagtc cggcaagggt
     2161 cgaatatctg atcgatatgc cgacggaact gttgcagaaa gctgaaggcg gtgttttata
     2221 tgtgggcgat attgcccaat acagccgcaa tatccaaacc ggcattgcct ttatcatcgg
     2281 caaggcagaa cgctgccgcg tcagggtggt cgcatcgggc agcagggcgg caggttcaga
     2341 cggcattgcc tgcgaggaaa agctggcgga actgctgtcg gaatcggtcg tccgtattcc
     2401 gccgctgcgt atgcagcatg aagacattcc cttcctgata caggggattg cctgcaatgt
     2461 ggcggaaagc caaaagattg cgcctgcctc attcagtgaa gaggcacttg ccgcattgac
     2521 ccgttacgac tggccgggaa atttcgacca actgcaaagc gtcgttgcaa cgctgttgtt
     2581 ggaggcggac ggacaggaaa tcggcgcagg ggcggtttct tcccttttgg ggcagaatgt
     2641 gcctgccgag ggagcggaag atatggtggg cgggtttaat ttcaacctgc ccctgcgcga
     2701 attgagggag gaggtggagc ggcgttattt cgagtaccac atcgcccaag aaggtcagaa
     2761 tatgagcaaa gtggcgcaga aagtcggttt ggaacgcacg cacctttacc gcaaactcaa
     2821 acagctcggc atcggcgttt cgcgccgggc gggggaaaaa accgaagaat aggcccggac
     2881 ggccggtttt ccggctgcgg gcttttgttt tcagacggca tttggtgcaa atgccgtctg
     2941 aaatcgtaag gggacggatt ttatgacaga ggacgaacgt ttcgcgtggc tgcaattggc
     3001 gtttacgccc tatatcggcg cggaaagctt cctgctgctg ctgcgccgtt tcggcagcgc
     3061 gtcaaacgcc ctgtccgcac cggcggaaca ggtcgccccg gcggtgcggc acaagcaggc
     3121 ggccgaggct tggcgcaatg cggaaaaacg cgcctcggcg cggcaggcgg cagaagcggc
     3181 gttggaatgg gaaatgcggg acggatgccg tctgatgctg cttcaggatg aagatttccc
     3241 cgaaatgctg acgcaggggc tgaccgcgcc gccggttttg tttttgcgcg gcaacgtgcg
     3301 gctgctgcac aagccttccg ccgccatcgt cggcagccgc catgccacgc cgcaggcgat
     3361 gcggattgcc aaagatttcg gcaagtcgtt gggcgggcaa aatatccccg ttgtgtcggg
     3421 tatggcttcc ggtatcgata ctgccgccca tcagggcgca ctggaggcag aaggcggcac
     3481 catcgccgtg tgggggacgg gtatagaccg catttacccg ccgtccaata aaaaccttgc
     3541 ctatgaaatc gccgaaaaag gattgattgt cagcgagttt cccatcggta cgcgcccgta
     3601 tgccggcaac tttccgcgcc gcaaccgcct gattgccgcg ctgtcgcagg ttacgctggt
     3661 ggttgaagct gcgttggaat ccggttcgct gattaccgcc ggcttggcgg cagaaatggg
     3721 gcgcgaagtg atggcggtac ccggttcgat agacaatccg cacagcaaag gctgccacaa
     3781 actgattaaa gacggcgcga aattggtgga atgcctggac gacatcctga acgaatgccc
     3841 ggggctattg caaaatacgg gtgcttcatc atattctata aataaggata cgcccgacac
     3901 gggcagtcgc gccgttcaga cggcatacgc cc
//
LOCUS       DAWHSK010000189         3929 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.189, whole genome shotgun sequence.
ACCESSION   DAWHSK010000189 DAWHSK010000000
VERSION     DAWHSK010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3929
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.189"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..2121
                     /locus_tag="WIC03_001695"
     CDS             <1..2121
                     /locus_tag="WIC03_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEZ3884164.1"
                     /translation="GKANAFTAPITVVNYDEQALNNTEARTLVDAVAKKDASVWQFGG
                     ESNTLTGLYFRGYQLDARQFSVNGLAGMYGTQGTASVQVGSAQLIKGASTAVNGMNPE
                     GAVSGSVNIETKKAADEGNRKIGLGRFSNNRAQGTFDLGQRFGENKAFGVRANGKLRH
                     GDTPRHGYSEDNKEFAVNADYRGETLRVAFDSIYAKRKTNGGRARMQDIQNAGGRLFG
                     APDGKTNLLPSWNWQNTAGQTNMLTFEWDAFDNAQITGGIGYNKARYYGTLISPTVCL
                     NATSTCTDMQNTNASGKVTARNYDYNTGTARLTDQYFRTLSMNLSARGEFETGPVTHN
                     WSTAFDRVIRQRKTINGSGNGNSKIEVKANENIANQLASFTADYPNSWENSANLDANI
                     KVNSLALSDTLGFVDNKYRLTLGGRFQAVEYTNKKKSQSGDAKRFSPMLMAAWVPQPD
                     LVVYGNYMEDLEPADIKTDDSGETTMAKPRVSRQFEVGVRKNWGNFVTTLNAFQIKRP
                     GYWRGHTTTKTTKGVTTTLTYGNNSDFARYKAQGGAAGDEQGMERNRGIEFNTYANLL
                     NNTLRPTLGLMYLQSTVKEYPNSRDMLVNGVQVANPRVIAKAGLEWDTPFAKGLTLNG
                     NVSYFGKSYQDTQKQYAFPSYTLIDVGARYKTKLGKNTLTVSSSVENLFNKNYWQVQR
                     GQYDRSFAVVGMPRTYWLKAELDF"
     gene            2245..3795
                     /locus_tag="WIC03_001696"
     CDS             2245..3795
                     /locus_tag="WIC03_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HEZ3884165.1"
                     /translation="MKKPLISVAAALLGVALGTPYYLGVKAEESLTQQQKILQEAGFL
                     TVESHQYERGWFTSTETTVIRLKPELLNNARKYLPDNLKTVLEQPVTLVNHITHGPFA
                     GGFGTQAYIETEFKYAPETEKVLERFFGKQVPVSLANTVYFNGSGKMEVSVPAFDYEE
                     LSGIRLHWEGLTGKTVYQKGFKSYRNSYDAPLFKIKLADKGDAAFEKAHFDSETSDGI
                     NPLALGSSNLTLEKFSLEWKEGVDYNVKLNELVNLVTDLQIGAFINPNGSIAPSKIEV
                     GKLAFSTKTGESGSFINSEGQFRFDTLVYGNEKYGPLDIHIAAEHLDASALTVLKRKF
                     AQISAKKMTEEQIRNDLIAAVKGEASGLFTNNPVLDIKTFRFTQPSGKIDVGGKIMFK
                     DMKKEDLNQLGLMLKKTEADIRMSIPQKMLEDLAVSQAGNIFSVNAEDEAEGRASLDD
                     INETLRLMVDSTVQSMAREKYLTLNGDQIDTAISLKNNQLKLNGKTLQNEPEPDFDEG
                     GMVSEPQQ"
BASE COUNT         1111 a          971 c          990 g          857 t
ORIGIN      
        1 ggcaaagcca acgcctttac cgcgccgatt accgtcgtca actacgacga acaagccctc
       61 aacaacaccg aagcgcgtac tttggtggat gccgtagcga aaaaagacgc ttccgtttgg
      121 cagttcggcg gagaaagcaa cacgctgact ggactgtatt tcagaggtta tcagcttgat
      181 gcgcgccaat tcagcgtcaa cggtttggca ggtatgtacg gcacgcaagg cacggccagc
      241 gtgcaagtcg gctccgcaca actgattaaa ggcgcgtcca ccgccgtaaa cggcatgaac
      301 cctgaaggtg cggtatccgg ttccgtcaat atcgaaacca aaaaagccgc cgatgaaggc
      361 aaccgcaaaa tcggtttggg ccggtttagc aacaaccgcg cccaaggtac gttcgatttg
      421 ggtcaacgct tcggcgaaaa caaagcattc ggcgtacgcg ccaacggcaa actgcgccac
      481 ggcgacactc cgcgccacgg ttacagcgaa gacaacaaag aatttgcggt aaatgccgac
      541 tatcgcggcg aaacactgcg cgtagcgttc gattccatct acgcaaaacg caaaaccaac
      601 ggcggtcgcg cgcgtatgca ggacattcaa aacgccggcg gacgcttgtt tggcgcgccc
      661 gatggtaaaa ccaacctgct gccttcttgg aactggcaaa ataccgccgg ccaaaccaat
      721 atgcttacgt ttgaatggga tgcgtttgat aatgcacaga ttacaggcgg tatcggctac
      781 aacaaggcac gctattacgg cacgctgatt tccccgaccg tatgtttgaa tgctaccagc
      841 acttgtaccg atatgcaaaa cacaaacgcc agcggcaaag taacggcacg caactatgac
      901 tacaataccg gtactgcgcg cctgaccgac caatacttcc gcactttaag catgaacctg
      961 tctgcgcgag gcgaatttga aaccggcccg gtaacacata actggagtac ggctttcgac
     1021 cgcgttatcc gccagcggaa aaccataaac ggcagcggga acggcaacag caagatagaa
     1081 gtgaaggcaa acgaaaatat tgcgaatcaa ttggcatcgt ttacagccga ctatcccaat
     1141 tcttgggaga actctgccaa cttggatgcc aatatcaaag tcaacagcct ggctttgtcc
     1201 gacaccttgg gcttcgtgga taacaaatac cgcctgactt tgggcggacg tttccaagcg
     1261 gttgaataca ccaataagaa aaaatcacag agcggcgatg ccaaacgctt cagcccgatg
     1321 ctgatggcgg cgtgggtgcc gcaacctgat ttggttgtgt acggcaacta tatggaagac
     1381 ttggagcctg ccgatatcaa aaccgatgat tccggcgaaa cgacaatggc gaaaccgcgt
     1441 gtcagccgtc agtttgaagt gggcgtgcgt aaaaactggg gcaatttcgt aaccacgtta
     1501 aacgcgttcc aaatcaaacg cccgggctac tggcgcggtc atacaactac caagacaaca
     1561 aaaggggtaa caacaacgct gacctatggc aacaattccg atttcgcccg ctataaggcg
     1621 caaggcggcg cggccggcga cgagcaaggt atggaacgca atcgcggtat tgagttcaac
     1681 acctacgcga acttgctcaa caatactttg cgtccgactt tgggtctgat gtatctgcaa
     1741 tcgaccgtga aagagtatcc aaattcacgc gatatgctgg ttaacggcgt acaagtcgcc
     1801 aatccgcgcg taattgccaa agcaggtttg gaatgggata caccgtttgc caaaggattg
     1861 actttaaacg gtaatgtttc gtatttcggc aagtcttacc aagacacgca aaaacaatac
     1921 gccttcccgt cctatacctt gattgacgta ggcgcgcgct acaaaaccaa gctcggcaaa
     1981 aatactttga ccgtcagcag ctcggtggaa aacctgttca acaaaaacta ttggcaggta
     2041 cagcgcggcc aatacgaccg cagcttcgcc gtcgtcggta tgccgcgcac ttactggctg
     2101 aaggcggaat tggatttctg atttccctat atccggaaaa tgccgtccga acaggtttca
     2161 gacggcattt cttttaaaga gatatggcgg gatacgctta aataatgcta ttctagcgtc
     2221 tttgaaacct gcttgtttga agtgatgaaa aaacctttga tttcggttgc ggcagcattg
     2281 ctcggcgttg ctttgggcac gccttattat ttgggtgtca aagccgaaga aagcttgacg
     2341 cagcagcaaa aaatattgca ggaagcgggc ttcttgaccg tcgaatcgca ccaatatgag
     2401 cgcggctggt ttacctctac ggaaacgacg gtcatccgct tgaaaccgga gttgctgaat
     2461 aatgcccgga aatacctgcc ggacaacctg aaaacagtgt tggaacagcc ggttacgctg
     2521 gtaaaccata tcacgcacgg ccctttcgcc ggcggattcg gcacgcaggc gtacattgaa
     2581 accgagttca aatacgcgcc tgaaacggaa aaagttctag aacgcttttt tggaaaacaa
     2641 gtcccggttt cccttgccaa taccgtttat tttaacggca gcggtaaaat ggaagtcagt
     2701 gttcccgcct tcgattatga agagctgtcg ggcatcaggc tgcactggga aggattgacg
     2761 ggaaaaacgg tttatcaaaa aggtttcaaa agctaccgca acagctatga tgcgcccttg
     2821 ttcaaaatca agctggcaga caaaggcgat gccgcgtttg aaaaagcgca tttcgattcg
     2881 gaaacttcag acggcatcaa tccgcttgct ttgggcagca gcaatctgac cttggaaaaa
     2941 ttctccctag aatggaaaga gggtgtcgat tacaacgtca agttaaacga actggtcaat
     3001 cttgttaccg atttgcagat tggcgcgttt atcaatccca acggcagcat cgcaccttcc
     3061 aaaatcgaag tcggcaaact ggctttttca accaagaccg gggaatcagg ctcatttatc
     3121 aacagtgaag ggcagttccg ttttgacacg ctggtgtacg gcaatgaaaa atacggcccg
     3181 ctggacatcc atatcgccgc cgaacatctc gatgcttctg ccttaaccgt attgaaacgt
     3241 aagtttgcac aaatttccgc caaaaaaatg accgaggaac aaatccgcaa tgatttgatt
     3301 gccgccgtca aaggcgaggc ttccggactg ttcaccaaca atcccgtatt ggacattaaa
     3361 acttttcgtt tcacacagcc gtcgggaaaa atcgatgtgg gtggaaaaat catgtttaaa
     3421 gacatgaaga aggaagattt gaaccaattg ggtttgatgc tgaagaaaac cgaagccgac
     3481 atcagaatga gtattcccca aaaaatgctg gaagacttgg cggtcagtca ggcaggcaat
     3541 attttcagcg tcaatgccga agatgaggcg gaaggcaggg caagtcttga cgacatcaac
     3601 gagaccttgc gcctgatggt ggacagtacg gttcagagta tggcaaggga aaaatatctg
     3661 actttgaacg gcgaccagat tgatactgcc atttctctga aaaacaatca gttgaaattg
     3721 aacggtaaaa cgttgcaaaa cgaaccggag ccggattttg atgaaggcgg tatggtttca
     3781 gagccgcagc agtaatgggt caaatgccgt ctgaaatgtt caggcggcat tttgttgtat
     3841 tggaaactgc cgttttcaac cattccttta gataagccgt gtcggattcg tttttatagt
     3901 ggattaaatt taaaccagta cggcgttgc
//
LOCUS       DAWHSK010000190         3910 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.190, whole genome shotgun sequence.
ACCESSION   DAWHSK010000190 DAWHSK010000000
VERSION     DAWHSK010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3910
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.190"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            95..1174
                     /locus_tag="WIC03_001697"
     CDS             95..1174
                     /locus_tag="WIC03_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemoglobin-haptoglobin-utilization protein"
                     /protein_id="HEZ3884166.1"
                     /translation="MKYKALSLLPLAAALAACAGGVAEPHVPVSIPTATPLPKGEVTL
                     SSDNGNIENINTANTETHPPRRTRRSLYASPQNTSSGISIQQREVEKIYFGVKSPEKS
                     FIFQTPGGAQYALSSYADPIVPSYSSPDFKIPDRHAGQRLADGSRIFICCSDSGATNQ
                     AEITKQDYMKFGAWIGPNGEIDLFAGGFPVGKTPASSSYYSNITLETAGKGKITYQVW
                     GIRVRNGQFVTSSYTPPKNSGYYSSTPTNTPVLSFITANFNSNKLAGEIRGNSDYGPS
                     VKIENATINSLSFSGTATSGGKNGKLEGKFFGKFNSTRSSDTGIGGKITFKDDRSLDT
                     VFGGVIYEKKLDDKTSQDTNHLKKQ"
     gene            1202..3628
                     /gene="hupB"
                     /locus_tag="WIC03_001698"
     CDS             1202..3628
                     /gene="hupB"
                     /locus_tag="WIC03_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemoglobin-haptoglobin-utilization protein HupB"
                     /protein_id="HEZ3884167.1"
                     /translation="MPIPFKPVLAAAAIAQAFPAFAADPAPQSAQTLNEITVTGTHKT
                     QKLGEEKIRRKTLDKLLVNDEHDLVRYDPGISVVEGGRAGSNGFTIRGVDKDRVAINV
                     DGLAQAESRSSEAFQELFGAYGNFNANRNTSEPENFSEVTITKGADSLKSGSGALGGA
                     VNYKTKSASDYVSEEKPYHLGIKGGSVGKNSQKFSSITAAGTWLGLDALMVYTRRFGK
                     ETKNRSTEDDVKIENKGYIFSPTQPFSKYQTYVATGVARSRPDPQEWVNKSTLFKLGY
                     NFNDRNRIGWIFEDSRTDRFTNELSNLWTGTIYSPATGDFRHRQDVSYRRRTGVEYKN
                     ELEHGPWDSLKLRYDKQRIDMNTWTWDIPKNYDKRGINGEVYHSFRHIRQNTAQWTAD
                     FEKQLDFSKAVWAAQYGLGGGKGDNANSDYSYFAKLYAPKILASNQAKITMLIENRSK
                     YKFAYWNNAFHLGGNDRFRLNAGIRYDKNSSSAKDDPKYTPAIRGQIPHLGSERAHAG
                     FSYGTGFDWRFTKHLHLLAKYSTGFRAPTSDETWLLFPHPDFYLKANPNLKAEKAKNW
                     ELGLAGSGKAGNFKLSGFKTKYRDFIELTYMGVSSDDKNNPRYAPLSDGTALVSSPVW
                     QNQNRSAAWVKGIEFNGTWNLDSIGLPKGLHTGLNVSYIKGKATQNNGKETPINALSP
                     WTAVYSLGYDAPSKRWGINAYATRTAAKKPSDTVHSNDDLNNPWPYAKHSKAYTLFDL
                     SAYLNIGKQVTLRAAAYNITNKQYYTWESLRSIREFGTVNRVDNKTHAGIQRFTSPGR
                     SYNFTIEAKF"
BASE COUNT         1077 a         1222 c          883 g          728 t
ORIGIN      
        1 tttttgcacc gacccacaaa accagttgac atcatataac atttaaactg ataatcaaaa
       61 ctattattaa cacatcatca agattgagta tgcgatgaaa tacaaagccc tgtccttact
      121 gccgcttgcc gctgcccttg ccgcctgtgc cgggggggta gccgaaccgc acgtccccgt
      181 gtccatcccc accgccacgc cgctgcccaa aggcgaggta acgttatcaa gcgataacgg
      241 caatatcgaa aacatcaaca ccgccaacac tgaaacacat ccgccgcgcc gcaccagacg
      301 ctccctctat gcctccccgc aaaacacatc ctccggtatt tccattcagc agcgggaagt
      361 agaaaaaatc tatttcggtg ttaaatcacc agaaaaatca ttcatcttcc aaacacccgg
      421 cggcgcgcaa tacgcccttt catcctacgc cgaccccatc gtccccagct actcctcccc
      481 cgatttcaaa attcccgacc gccacgcagg gcagcggctt gccgacggca gccgcatctt
      541 tatctgctgc agcgactccg gggcaaccaa tcaagcggaa attaccaaac aagattatat
      601 gaaattcggg gcgtggatag gtcccaacgg cgaaatcgac ctcttcgccg gcggcttccc
      661 cgtcggcaaa acaccggcat caagcagtta ttacagcaac atcacgctcg aaaccgccgg
      721 caagggcaaa atcacttatc aggtttgggg catccgcgtc agaaacgggc aatttgttac
      781 ttcctcttat acgccgccga aaaacagtgg ttattattcg tccacaccta ccaacacccc
      841 cgtcctttcc tttatcaccg ccaatttcaa cagcaacaaa ctggcaggcg aaatccgcgg
      901 caacagcgat tacggaccga gtgtgaaaat tgaaaacgca acgattaaca gtttatcctt
      961 ttccggcact gccacctccg gcggcaaaaa cggcaaattg gaaggcaagt tttttggcaa
     1021 gttcaacagc acccgcagca gcgacaccgg cataggcggc aaaatcactt ttaaagacga
     1081 ccgctccctc gataccgtat tcggcggcgt gatttatgag aaaaaacttg atgacaaaac
     1141 cagtcaagat accaaccatc tcaaaaaaca ataacccatc cgcttttaaa aaggttttct
     1201 tatgcccatc ccctttaaac ccgtattggc tgccgccgcc atcgcccaag cgtttcccgc
     1261 ctttgcggca gaccccgcgc cgcagtccgc ccaaacgctg aacgaaatca ccgttaccgg
     1321 cacgcacaaa acccaaaaac tcggcgaaga aaaaatccgc cgcaaaacct tagacaagct
     1381 cttggtcaac gacgaacacg acctggtgcg ctacgacccc ggcatttccg tcgtcgaagg
     1441 cggcagggcg ggttctaacg gctttaccat acgcggcgtg gacaaagacc gcgtcgccat
     1501 caacgttgac gggctggcgc aggcggaaag ccgctcttcc gaagccttcc aagaattgtt
     1561 cggcgcgtac ggcaacttca acgccaaccg caacacttcc gagccggaaa acttttccga
     1621 agtaaccatc accaaaggcg cggactcgct caaatccggc agcggcgcat tgggcggtgc
     1681 cgtcaactat aaaaccaaat ccgcaagcga ctacgtttcc gaagaaaaac cttaccactt
     1741 ggggataaag ggcggcagtg tcggcaaaaa cagccaaaaa ttcagcagca taactgccgc
     1801 aggaacttgg ttgggtctgg acgcattgat ggtctacacc cgccgcttcg gtaaagaaac
     1861 caaaaaccgc tcgaccgagg acgatgtcaa aattgaaaat aagggatata ttttcagccc
     1921 aacccaacca ttcagcaagt accagaccta tgttgccaca ggggttgcgc gctcccgccc
     1981 cgacccgcaa gaatgggtaa acaaaagcac cctgttcaag ctgggctaca atttcaacga
     2041 ccgcaaccgc atcggctgga tttttgaaga ctcgcgcacc gaccgcttta ccaacgagct
     2101 gtctaatttg tggacgggta caatttactc tcctgcaacg ggcgacttcc gtcaccgcca
     2161 agacgtgagc taccgccgcc gcaccggcgt cgaatacaaa aacgaattgg aacacggccc
     2221 gtgggacagc ctcaagctgc gctacgacaa gcagcgcatc gatatgaaca cttggacttg
     2281 ggacatcccg aaaaattacg ataaaagagg catcaacggc gaggtttacc attcgttccg
     2341 gcatatccgc caaaacaccg cgcaatggac tgccgatttt gaaaaacaac tcgacttttc
     2401 caaagccgtt tgggcggcgc aatacggctt gggcggcggc aaaggggaca atgccaactc
     2461 ggattacagc tatttcgcaa aactgtacgc ccccaaaatc ctcgcttcca accaagccaa
     2521 aatcacgatg ctgatcgaaa accggtcgaa atataaattt gcctattgga acaatgcgtt
     2581 tcacttgggc ggcaacgacc gcttccgcct gaatgcgggc atacgctacg acaaaaacag
     2641 cagcagcgcg aaagacgatc cgaaatacac ccccgccatc cgggggcaga ttccccattt
     2701 aggttcggaa cgcgcgcacg cgggcttcag ctacggcacg gggttcgact ggcggtttac
     2761 caagcatctg cacttgttgg caaaatacag caccggcttc cgcgcaccga cttcggacga
     2821 aacctggctg ctgttcccac accccgattt ctacttgaaa gccaacccaa acctgaaagc
     2881 ggaaaaagcc aaaaactggg aattgggtct ggcgggcagc ggcaaagcgg gcaacttcaa
     2941 gctctcgggc ttcaaaacca aataccgcga ctttatcgaa ttgacgtata tgggcgtttc
     3001 gtcagacgat aaaaacaacc ccagatacgc cccgctttca gacggtacgg cattggtcag
     3061 ctcgcccgtt tggcaaaacc aaaaccgctc cgccgcctgg gtgaaaggca tagagtttaa
     3121 cggcacgtgg aacctcgaca gtatcggttt gcccaaaggg ctgcacaccg gcctcaacgt
     3181 cagctacatc aagggcaagg caacgcaaaa caacggcaaa gaaacgccca tcaacgcgct
     3241 ttcgccgtgg acggcggttt acagcctggg ctacgacgct ccttccaaac gttggggcat
     3301 caacgcctac gccacgcgca ccgccgccaa aaagccgtcc gacaccgtcc acagcaacga
     3361 cgacttgaac aacccgtggc cttatgccaa acacagcaag gcctatacgc tgttcgacct
     3421 ttccgcctac ctcaacatcg gcaaacaggt tacgctccgc gccgccgcgt acaacattac
     3481 caacaagcag tactacactt gggaatcttt acgcagcatc cgcgagttcg gcacggtcaa
     3541 ccgcgttgac aacaaaaccc acgccggcat ccaacgcttt acctcgccgg gcaggagcta
     3601 caatttcacc atcgaagcga agttctaagc tcctgatgtg ccaatgccgt ctgaaacccg
     3661 atgatttggg tttcagacgg cattttttac ggtcgcaccg ccgccccctt tcaggcttgt
     3721 accaagccca atcccgcaac tctccgtcat tcccgcaact ctccgtcatt cccacgaaag
     3781 tgggaatcta gaaacgcaag gctgcaagaa tttatcggaa atgactgaaa ctcaacgaac
     3841 ctggattccc gcctgcgcgg gaatgacgaa gctatccgca cagaaacctg caccacgtca
     3901 ttcccacgaa
//
LOCUS       DAWHSK010000191         3862 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.191, whole genome shotgun sequence.
ACCESSION   DAWHSK010000191 DAWHSK010000000
VERSION     DAWHSK010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3862
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.191"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..465
                     /locus_tag="WIC03_001699"
     CDS             <1..465
                     /locus_tag="WIC03_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEZ3884168.1"
                     /translation="FKTEDVAENLARRFPGLEFDRMKINSQLLLQTILGRFSVSSDNA
                     GKQFFEDHKTYVPARFTDYAAAFVEHGAEAFVRPASRYNESTPSFGYGHLYIMRQLSR
                     PTSKQALIETVAENLNIVSTTPDGLTFHPPAEVYVEEILADLADRHFLVSAD"
     gene            547..1644
                     /locus_tag="WIC03_001700"
     CDS             547..1644
                     /locus_tag="WIC03_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEZ3884169.1"
                     /translation="MNQTFTLPDTRPYPQNPIKNHLLLNAYQLAHNSSQASRKLSSGQ
                     LQTEIRGMLEQNHYINLSLALTMSPDAGTYAALLSSVNAVLDCEKEGEVQWFALPVVL
                     VSGCKKERAIEMKLPTEALFACLQNYPHLRVLTQETQWLPYLVHSSDLSAVAPDEWWR
                     AKQNTEAAAQHLRRFAPRPLLLPEGQSVHVVYALGFGSGKVQTALGQNLLQAGLPLMQ
                     VWQENLASEGVTLFANPLSPDSPVRALSEGSHTRQRMAMDVFAANAIRAVRMQSPRVG
                     VVAAAKVGGQILFGFNATDGAFEVVPQVFSWQLSFTDNIAVVQQNFLDLMAECRVEHV
                     YLLHNPLGEQESIPSYAEALKREGHNPFFSA"
     gene            1758..2873
                     /locus_tag="WIC03_001701"
     CDS             1758..2873
                     /locus_tag="WIC03_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEZ3884170.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQAIADVECLLCPVGDG
                     GEGTVDAIRHSLDLEEKCLQVTGPFGQKEVMRYFQKEQLALFEVADLVGLGKIPLEKR
                     NPLQIQTRGIGELIRHLISQEIKEIYIGVGGTASNDGGIGIAAGLGYQFYDEDGNTLP
                     TCGQALLNLASVSTENRYEIPEDVHIRILADVVSPLCGHQGATYTFGKQKGLDSTMFE
                     AVDQAIQDFYEKVSPATLKLKGAGAGGGIAGGLCAFAQASIVSGIDTCLDLIDFDKKV
                     SDVDLVIIGEGRLDRQSLAGKAPIGVAKRTPVGVPVVAICGSLVEDLPSLPFENIQAA
                     FSILEKSEPLEDSLKNASLYLEHTASNIGHLLNMPKI"
     gene            2998..3453
                     /locus_tag="WIC03_001702"
     CDS             2998..3453
                     /locus_tag="WIC03_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEZ3884171.1"
                     /translation="MKKPKILFVCLGNICRSPMAEYILRRRAAEAGIPLEADSAGTSG
                     WHDGEDMHRETAKILKKHGIDASGFTSRKIRQSDAAAFDCIIAMDGKNLSELERTFGR
                     RPEKIFKLTDLIPESGYDHVPDPWYTGDFEETFRLADAGCRALLEKISK"
     gene            3524..>3862
                     /locus_tag="WIC03_001703"
     CDS             3524..>3862
                     /locus_tag="WIC03_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEZ3884172.1"
                     /translation="MKTNFKQKIIEQARSEGLQVTALREQVLDIVLQQSGVIKAYNVL
                     SQMQQQSEGVLAPPTAYRALDFWAEQGVLHKVAAVNGYILCSHAQHECDDHCHDHEEA
                     EAHHSAFILVC"
BASE COUNT         1003 a          915 c          995 g          949 t
ORIGIN      
        1 tttaaaacgg aagatgttgc ggaaaacctc gcccgccgat ttcccggtct ggaatttgac
       61 cgcatgaaaa tcaattccca gcttttattg caaaccattc tcgggcgttt ttctgtttca
      121 tcagacaatg cgggcaaaca attttttgaa gaccataaaa cctatgtgcc ggcgcgtttt
      181 acagattatg ccgccgcctt tgtggaacat ggcgcggaag cgtttgtccg gcctgccagc
      241 cgttacaacg aaagcacgcc ctcattcgga tacgggcatt tgtacattat gcggcaatta
      301 tcgcggccga cgagcaaaca ggcattgatt gaaacggttg ccgaaaactt gaacatcgtc
      361 agcaccacgc ccgacggttt gacattccat ccgcctgccg aagtgtatgt ggaagaaata
      421 ttggcagact tggcagacag gcattttctc gtttcggcgg attgacgggc aagtcccgac
      481 ggcaatccgt ccgatatatt ttcaaacagg aaagacacac actgcgaaaa acggtacacg
      541 acacatatga accagacatt taccttgccc gatacgcgcc cgtatcccca aaatccgatt
      601 aagaaccacc tgctgctcaa tgcctaccag ttggcgcaca attcttccca ggcttcgcgc
      661 aaactctcgt ccggccaact tcaaaccgaa atcaggggga tgcttgagca aaaccactat
      721 atcaaccttt ccctcgcgtt gacgatgtcg cccgatgccg gaacttatgc cgcgctgctt
      781 tccagtgtga acgcggtact cgattgcgag aaagaaggcg aagtgcagtg gttcgccctg
      841 ccggtcgtgc tggtgtccgg ctgcaagaaa gaacgggcaa tcgagatgaa gctgccgacg
      901 gaggcattgt ttgcctgcct gcaaaactat ccgcacctgc gcgttttgac gcaagagaca
      961 caatggctgc cttatcttgt gcattcttcc gatttgagtg cggtcgcgcc ggatgagtgg
     1021 tggcgtgcca aacaaaatac cgaagcggcg gcgcaacact tacgccgttt cgccccgcgt
     1081 cctttgctgt tgcccgaagg acagtccgtc cacgtcgttt acgcgctggg tttcggcagc
     1141 ggtaaggttc agacggcatt gggtcagaac cttcttcagg caggcctgcc cctgatgcag
     1201 gtatggcagg aaaatcttgc atcggaaggc gttacgctgt ttgccaaccc gctttccccg
     1261 gattctccgg tacgcgcgct ttcagagggc agccacacgc gccaacgtat ggcgatggat
     1321 gtttttgcgg caaacgcgat acgcgccgtc cgtatgcaga gcccgcgcgt cggcgtggtc
     1381 gctgcggcaa aggtaggtgg acagattcta ttcggattta atgcgaccga cggcgcgttt
     1441 gaagtcgtgc cgcaggtgtt ttcgtggcag ctttccttta ccgacaatat cgccgttgtt
     1501 cagcaaaatt ttttggattt aatggcagaa tgccgtgtag aacacgttta cctgttgcac
     1561 aatcccttgg gcgaacagga aagcatcccg agctatgcgg aagcattgaa acgggaaggg
     1621 cacaatccgt ttttcagcgc atagtgattt gtagtagtga taggctttct catttctata
     1681 ataaatcaaa ataaagagca caacactttt tcattctgtg ttgtgccttg agtgaaacga
     1741 aaggatgaat tataaatatg aagattgtaa ttgcaccgga ttcgtttaag gaaagcttga
     1801 cagctcaaca ggtagctgaa gcaataaaaa gaggcttcca acaagcgata gcagatgtgg
     1861 aatgtctcct ctgtcctgtc ggtgatgggg gagaaggcac tgtagatgct atccgacatt
     1921 ctcttgacct agaagaaaaa tgtctccaag tgacaggacc ttttggacaa aaagaagtca
     1981 tgcgctattt tcaaaaagaa caactagccc tattcgaagt tgctgatttg gttggtcttg
     2041 gaaaaatccc gctagagaaa cgaaatccat tacaaatcca aactcgtggt attggagaat
     2101 tgattcgcca cctcattagt caagaaatta aagaaatcta tattggcgtt ggcggtacgg
     2161 ccagtaatga tggaggtatt gggattgctg ctggtttagg ttatcaattt tatgatgagg
     2221 atggaaatac cttgcccact tgcggtcaag ccttactaaa cctagcttct gtttcaacag
     2281 aaaatcgcta tgaaattcct gaagatgttc acattcgtat tttagcagat gttgtgagtc
     2341 ccttatgtgg acaccaaggt gcgacctata cgtttggcaa gcaaaaaggg ttggattcta
     2401 ctatgtttga ggccgtagat caggcaatac aagattttta tgaaaaagtc tcccctgcaa
     2461 cattaaaact taaaggagca ggagctggtg gaggcatcgc tggtggtttg tgcgcctttg
     2521 ctcaggcaag tatcgtatct ggaattgaca cctgcctgga cttgattgac tttgataaga
     2581 aagtgtcaga tgttgacttg gttatcattg gtgaaggaag actagatcgt caaagtttag
     2641 cagggaaagc gcctattggt gtagcaaaaa gaacccctgt cggagttcct gttgtcgcta
     2701 tttgtggcag tctcgttgaa gatttgcctt ccctgccatt tgaaaatatc caagctgcct
     2761 tttctatttt ggagaaaagt gaacctttag aagatagttt gaaaaatgcc agtctctatt
     2821 tggagcatac ggcttctaat attggacact tattaaatat gcctaagatt tagccaaacc
     2881 atttcttcca gatggatgtt ttggctggtt ctgccttatg cggttacgat taaccgaccg
     2941 atgacgtctg aaagggtttc cgcttcagac ggcatcgcgc cggaacaggg aggggatatg
     3001 aaaaaaccga aaatcctttt tgtctgcctc ggcaacatct gccgttcgcc gatggcggaa
     3061 tacattttgc gccgccgcgc cgccgaagcg ggcattcccc ttgaagcgga cagcgcgggg
     3121 acatcgggct ggcacgacgg cgaagatatg caccgcgaga cggcaaagat attgaaaaaa
     3181 cacggtatcg atgcttcagg ctttaccagc cgcaaaatcc gccaaagcga tgcggcggcg
     3241 tttgactgca tcatcgcgat ggacggcaag aatttgtccg aattggaaag aaccttcggc
     3301 aggcggccgg aaaaaatatt caagctgacc gacctgatac ccgaaagcgg ttacgaccat
     3361 gtccccgatc cgtggtatac gggcgatttt gaagaaacct tcaggcttgc ggatgcgggc
     3421 tgtcgggcat tgttggaaaa gatttccaaa taaagcaatt gaatacaaat ataaaaccgc
     3481 ctcctgccgt atcggttgtt cagacggcat aaacagagaa tttatgaaaa caaatttcaa
     3541 acagaaaatt atcgaacagg cacgcagcga aggcttgcag gtaaccgctt tgcgcgagca
     3601 ggtattagat attgtcttgc agcaaagcgg cgtgattaaa gcctacaacg tcttgtcgca
     3661 gatgcagcag caaagcgagg gcgtgcttgc accgcctacc gcctaccgcg cccttgattt
     3721 ttgggcggag cagggcgttt tgcacaaagt ggcggcggtc aacggctata ttttgtgcag
     3781 ccacgcgcag cacgagtgcg acgaccattg ccacgaccac gaagaagccg aagcgcacca
     3841 cagcgcgttt attttggtct gc
//
LOCUS       DAWHSK010000192         3861 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.192, whole genome shotgun sequence.
ACCESSION   DAWHSK010000192 DAWHSK010000000
VERSION     DAWHSK010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3861
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.192"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            490..1446
                     /gene="thiL"
                     /locus_tag="WIC03_001704"
     CDS             490..1446
                     /gene="thiL"
                     /locus_tag="WIC03_001704"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEZ3884173.1"
                     /translation="MKEFDFIKRYLQTGTDKDVVLGIGDDAAIVRPREGFDLCFSADM
                     LLKDRHFFADVKPEDLAWKVLAVNISDMAAMGAIPRWVLLSAALPELDEVWLERFCGS
                     FFGLAKKFGVTLIGGDTTKGDMAFNVTIIGELPKGRALRRDAAVAGDDIWVSGRVGMA
                     AAALNCRLKRCVLPDDVFAECEQKLLRPEPRVGLGLALLPFARAAQDVSDGLAQDLGH
                     ILTASGVGAEIWADSLPSLSVLKDILPRAQWLSYTLAGGDDYELVFTAPESCRSRVLD
                     AAERCGVPVTRIGKINGGCRLKVLDADGRELELHSLGFDHFG"
     gene            1439..1924
                     /locus_tag="WIC03_001705"
     CDS             1439..1924
                     /locus_tag="WIC03_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEZ3884174.1"
                     /translation="MADFKPDFAWLLKRPLCFLAFGFGSGLAPFAPGTFGTLAALPLA
                     FVLILLGIDGLLLAFLCIVLFMWGIRICAYAERETGVSDHGGIVWDEIVAMLFVLAFV
                     PFRWTWWLAAFVLFRLFDALKPSPVGWFDKDLHGGLGIMADDMAAAVMTLIVLRIAML
                     F"
     gene            complement(1984..3660)
                     /gene="ettA"
                     /locus_tag="WIC03_001706"
     CDS             complement(1984..3660)
                     /gene="ettA"
                     /locus_tag="WIC03_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEZ3884175.1"
                     /translation="MSQQYVYSMLRVSKVVPPQKTIIKDISLSFFPGAKIGLLGLNGA
                     GKSTVLRIMAGVDKEFEGEAVPMGGIKIGYLPQEPELDPEKTVREEVESGLGEVAAAQ
                     KRLEEVYAEYANPDADFDALAEEQGRLEAIIAAGSSTGGGAEHELEIAADALRLPDWD
                     AKIGHLSGGEKRRVALCKLLLSKPDMLLLDEPTNHLDAESVEWLEQFLVRFPGTVVAV
                     THDRYFLDNAAEWILELDRGHGIPWKGNYSSWLEQKEKRLENEAKSEAARVKAMKQEL
                     EWVRQNAKGRQAKSKARLARFEEMSNYEYQKRNETQEIFIPVAERLGNEVIEFVNVSK
                     SFGDKVLIDDLSFKVPAGAIVGIIGPNGAGKSTLFKMISGKEQPDSGEVKIGQTVKMS
                     LIDQSREGLQNDKTVFDNIAEGRDILQVGQFEIPARQYLGRFNFKGSDQSKIAGQLSG
                     GERGRLHLAKTLLSGGNVLLLDEPSNDLDVETLRALEDALLEFAGSVMVISHDRWFLD
                     RIATHILACEGDSKWVFFDGNYQEYEADKKRRLGEEGTKPKRIKYKPVTR"
BASE COUNT          800 a          947 c         1065 g         1049 t
ORIGIN      
        1 gaggcaacgc tgtactggtt taaatttaat ccactatatc ggttgaaact ctgattttaa
       61 ggcggtagga tgtgggtttg cccatagaaa gggaatcctt tctgtatcaa gccctgaaag
      121 ggataattca tacaaattca cgcctttccc cctcattggg aaatggatgg aatcgtgcca
      181 gatgtgtgcg gcactgtatg ccggatatgg ttttatcatc agcccttttc ggttgaaacc
      241 tcgccagttg cagcgattga gcctaatcgg tggcggaagt tgccgctttg cattcggggt
      301 ggcgtgcagt gcggtgcttt gatatgccgt ttgtgtgttg aaacagggtg gtcggtgcat
      361 acgggtacgg tatggccaaa gctaaaagtg aaatacgctg aaacactgaa tgagccgctt
      421 tattgtttgt acggcctttg ctgccttgct atgatttaaa ttggattcgc ccgccggaca
      481 ttttgcgaca tgaaagaatt cgacttcatc aaacggtatt tgcaaacagg cacggataag
      541 gatgtcgtat tgggcatagg cgacgatgcg gcgattgtcc gtccgcgtga aggcttcgat
      601 ttgtgtttca gtgcggatat gcttttgaag gacaggcatt tttttgcaga tgtcaaacct
      661 gaagacttgg cttggaaggt tttggccgtc aatatttcag atatggcggc gatgggcgcg
      721 ataccgcgtt gggtgctgct gagcgcggcg ttgcccgaat tggatgaagt atggctggaa
      781 cggttttgcg gcagcttttt cggtttggca aaaaagtttg gcgtaacgtt aatcggcgga
      841 gatacgacca agggcgatat ggcgttcaat gtaaccatta tcggcgaatt gccgaagggt
      901 agggcattgc ggcgtgatgc ggcggttgcg ggtgacgata tttgggtgtc ggggcgtgtc
      961 ggtatggcgg cggcggcttt gaactgccgt ctgaaacggt gtgtgttgcc ggatgatgtg
     1021 tttgccgaat gcgaacaaaa gctgctccgc cccgagccaa gggttgggct ggggcttgcg
     1081 ctgttgccgt ttgcccgggc ggcgcaggat gtttcagacg gcctcgcgca agatttgggg
     1141 catatcctga ccgcttccgg cgtgggggcg gaaatttggg ccgattcgct gccgtcttta
     1201 tccgtattga aagatatttt gccccgagcg caatggctgt cttatacttt ggcgggcggc
     1261 gacgattacg agctggtgtt taccgcgccg gaaagttgcc gcagccgcgt gcttgatgcg
     1321 gcagaacggt gcggcgtgcc ggtaacgcgc atcggcaaaa tcaacggagg atgccgtctg
     1381 aaggttttag atgctgacgg cagggaattg gaactacatt ctttaggatt cgatcatttt
     1441 ggctgatttt aaacctgact ttgcgtggct gttgaaacga ccgttgtgtt ttttggcttt
     1501 tggtttcggc agcgggctgg ctccgttcgc gccgggtaca tttggcactt tggcggcact
     1561 gcctttggca tttgtgctga ttttgctcgg catagacggg ctgctgctgg cttttttgtg
     1621 tatcgtgctg tttatgtggg gcatacgcat ttgcgcttat gcggaacgtg aaacgggtgt
     1681 cagcgaccat ggtgggattg tttgggacga gattgtcgcc atgctgtttg tgctggcgtt
     1741 tgtgccgttc aggtggacgt ggtggctggc ggcatttgtc ctattccgtc tgtttgacgc
     1801 gctcaaaccg tctcccgtcg gttggtttga caaggatctg cacggcggtt tgggcattat
     1861 ggcggacgat atggcggctg cggtgatgac tttgattgtc ttgaggattg caatgctgtt
     1921 ttaaacggtg ctgccttgta aaaatgccgt ctgaaagcct ttcagacggc attgtttcgg
     1981 aggttaacgc gttaccggtt tgtatttgat gcgtttcggt ttcgtgcctt cttcgccgag
     2041 tcggcgtttc ttgtcggctt cgtattcctg atagttgccg tcaaagaaca cccatttgga
     2101 gtcgccttcg caagccaaga tatgcgtagc aatacggtcg aggaaccagc ggtcgtgcga
     2161 aatcaccatc acgctgccgg caaattccag caatgcatct tccagcgcgc gcagggtttc
     2221 cacgtcgagg tcattagacg gttcatccag cagcaataca ttgccgccgc tcaacaaggt
     2281 tttcgccaag tgcaaacgtc cgcgttcgcc gcctgacaat tgacctgcga ttttgctttg
     2341 gtcgctgcct ttaaagttga aacgacccaa atattggcgg gcgggaattt caaactgacc
     2401 aacctgcaaa atgtcgcggc cttcggcgat gttgtcgaac acggttttgt cgttttgcaa
     2461 accttcgcgg ctttggtcaa tcaagctcat tttcacggtt tggccgattt tcacttcgcc
     2521 ggaatcaggc tgctctttgc ccgaaatcat tttgaacagc gtcgatttac ccgcgccgtt
     2581 cgggccgatg atgccgacaa tcgcgccagc aggtactttg aagctcaaat catcaatcag
     2641 cactttatcg ccaaacgatt tggaaacatt cacaaattca atcacttcgt tacccaaacg
     2701 ctcggcaacg ggaataaaga tttcctgcgt ttcattgcgt ttttggtatt cgtagttgct
     2761 catttcttca aaacgcgcca aacgcgcttt agacttggct tggcggcctt tggcattttg
     2821 gcgaacccat tccaattcct gcttcatcgc tttcacacgc gcagcttcgg attttgcctc
     2881 gttttccaag cgtttttctt tctgctccag ccaagacgag taattgcctt tccacggaat
     2941 accgtgtccg cggtcgagtt ccaaaatcca ttcggcggcg ttgtccaaga agtagcggtc
     3001 gtgcgttacc gcaaccaccg tgccggggaa gcgcaccaag aattgctcca gccattccac
     3061 tgattccgca tccaagtggt tggtcggctc gtccagcaaa agcatatcgg gtttgctcaa
     3121 caagagtttg cacaaagcca cacggcgttt ttcaccgccg gacagatggc cgattttggc
     3181 atcccaatca ggtaggcgca gcgcgtcggc agcgatttcc aattcatgct ctgcaccgcc
     3241 gcccgtggac gaacccgccg caataatcgc ttccaagcga ccctgctctt ccgccagcgc
     3301 gtcaaaatcc gcatcaggat tggcgtactc ggcatacact tcttccaaac gtttctgcgc
     3361 cgcagctacc tcgcccaaac cgctttccac ttcctcgcgc acggtttttt caggatcaag
     3421 ctccggctct tgcggcagat agccgatttt gatgccgccc atcggcacgg cttcgccctc
     3481 aaattcctta tccacacccg ccataatccg cagcacggtg gacttgcccg cgccgttcaa
     3541 accgagcaaa ccgattttcg cgccggggaa gaaagaaagg gaaatatctt taatgatggt
     3601 tttctgcggc ggcacaacct tgcttacgcg cagcatagaa tagacgtatt gttgggacat
     3661 agttttctcg tttcggtcgg acaaatttca gacggcacat tttaaccgat aatttgattt
     3721 aagccagttt atccgcgaac cggtattgcc aaaatcgggc aggattcata aaatccgcct
     3781 atccctttga aattatacaa acaaaaaaaa taataatgat aggggatcgc cgcaccggca
     3841 accatttcgg attttccaaa g
//
LOCUS       DAWHSK010000193         3853 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.193, whole genome shotgun sequence.
ACCESSION   DAWHSK010000193 DAWHSK010000000
VERSION     DAWHSK010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3853
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.193"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(80..898)
                     /locus_tag="WIC03_001707"
     CDS             complement(80..898)
                     /locus_tag="WIC03_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ3884176.1"
                     /translation="MKGMDKLRYQQDFLNIRPIFTAGEQEYLTELSDRLPLSVLTDSV
                     RNIEEIGIDFVYSSAKLEGNTYNQYDTQALLKLGQTAGGKLYSDAVMLINLRESYRHL
                     LSGLDSPEPFDWLDFLKTTHSLISENLLEKGSGGVVRRDSVTISGTDYTPLSNPQSLD
                     TELKWLLQEAPKIENPFDRAVYLHNNLAYLQYFKDCNKRTARNCMTLSLMRSGFFPCV
                     FSPDSYPAYAEAVVAYYETGDYGLFKKYFISAYENTVNKYGPQPDMDIFRNFSL"
     gene            complement(978..2228)
                     /locus_tag="WIC03_001708"
     CDS             complement(978..2228)
                     /locus_tag="WIC03_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEZ3884177.1"
                     /translation="MTKLTRRQIIRRTAGTLFALSPIASAVAKTVRAPQFTAARIWPS
                     HTYTRLTLESTTALKYQHFALDNPGRLVVDIQNANINTVLHGLSQKVAADDPFIRNIR
                     AGQNTPDTVRLVIDLKQPSYAQVFSLPPVGGFKNRLVVDLYPQGMDTDDPMMALLNGN
                     PNKTRRGSPEADLAQNTPAQTERGKNGRRPVIMIDPGHGGEDPGAVSPGGLQEKHVVL
                     SIARETKKQLEALGYNVFMTRNEDVFIPLGVRVAKGRARRADVFVSIHADAFTSPSAR
                     GTGVYMLNTKGATSSAAKFLEQTQNNADAVGGVPTSGNRNVDTALLDMTQTATLRDSR
                     KLGKLVLEELGRLNHLHKGRVDEANFAVLRAPDMPSILVETAFLSNPAEEKLLGSESF
                     RRQCAQSIASGVQRYINTSVLKRG"
     gene            complement(2225..2686)
                     /gene="tsaE"
                     /locus_tag="WIC03_001709"
     CDS             complement(2225..2686)
                     /gene="tsaE"
                     /locus_tag="WIC03_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEZ3884178.1"
                     /translation="MPDFPPVSRFLADEAATLDLGAAWSSRLNAPLVIYLEGDLGAGK
                     TTLTRGILRGLGHQGAVKSPTYAIVESYPLERFTLHHFDLYRFSFPEEWEDAGLDELF
                     AANSVCLIEWPQQGGEFTPPADITATLTHDGGGRKCLLTAHTERGRESLPL"
     gene            2873..3685
                     /locus_tag="WIC03_001710"
     CDS             2873..3685
                     /locus_tag="WIC03_001710"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEZ3884179.1"
                     /translation="MENIGRQRPIGVFDSGIGGLTNVRALMERLPMENIIYFGDTARV
                     PYGTKSKATIENFSMQIVDFLLEHDVKAMVIACNTIAAVAGQKIRQKTGNMPVLDVIS
                     AGAKAALATTRNNKIGIIATNTTVNSNAYARAIHRDNPDTLVRTQAAPLLVPLVEEGW
                     LEHEVTRLTVCEYLKPLLADGIDTLVLGCTHFPLLKPLIGREAHNVALVDSAITTAEE
                     TARVLAQEGLLNTDNNNPDYRFYVSDIPLKFRTIGERFLGRTMEQIEMVSLG"
BASE COUNT          841 a          956 c         1143 g          913 t
ORIGIN      
        1 ccgaaggtaa gggaggggcg tgtccatttt gccgccgcat ttgccgcgtg cggaagatac
       61 ggataacaac cccttggaat taaagggaga agttgcggaa aatatccata tcaggctgtg
      121 ggccgtactt atttacggta ttttcgtatg ccgaaataaa atatttcttg aataaaccgt
      181 aatcgccggt ttcgtaataa gctacgacag cctcggcata ggcgggataa ctgtcggggg
      241 agaatacgca aggaaaaaaa ccggagcgca tcagcgacaa ggtcatgcag tttctggcag
      301 tacgtttatt gcaatcttta aaatattgaa gatacgccaa attattatgg agataaacgg
      361 cgcggtcgaa aggattttct attttgggag cttcttgaag caaccatttt aattccgtat
      421 ccaaactttg cgggttagat aaaggggtgt agtctgtccc gcttatggta acgctgtcgc
      481 ggcggactac gccgccggag cctttttcca gtaagttttc ggaaatcagg ctgtgggtgg
      541 tttttaagaa atccaaccag tcaaacggct cggggctgtc caaaccggac agcaggtgcc
      601 ggtaactttc cctcaggttg attaacatta cggcatccga atataacttc ccgcctgcgg
      661 tttgcccgag tttaagcaat gcctgcgtat cgtattgatt ataagtgttt ccttccaatt
      721 tggcagaact gtacacaaaa tcgataccga tttcttcaat attacgcacg gagtccgtta
      781 aaacagataa aggaagccga tcggacaatt cggtcaggta ttcttgttcg ccggctgtaa
      841 aaatggggcg gatatttaaa aaatcctgtt ggtatctcaa tttatccata cctttcatca
      901 taaaaacgcg gtttggggcg gaagtaggtt tatgcctgcc ttcatccgac aactatattg
      961 aaacaccgcc ttttcaatca accccgcttc aatacggatg tattgatgta gcgttggaca
     1021 cccgaggcaa tggattgggc gcactgtcgg cggaaggatt cgctgcccag cagcttctct
     1081 tcggcagggt tggacaggaa ggcggtttcg accaggatag acggcatatc gggtgcgcgc
     1141 aaaacggcga aattggcttc gtccaccctg cctttgtgca gatggttgag cctgcccaat
     1201 tcttcaagca ccagtttgcc gagtttgcgg ctgtcgcgca gtgtggcggt ttgggtcata
     1261 tcgagcaggg cggtatcgac gttgcggttg ccgctggtcg gtacgccgcc gaccgcgtcg
     1321 gcattgtttt gtgtctgttc caagaatttg gcggcagagc tggtcgcgcc tttggtgttt
     1381 aacatataaa cccccgtgcc gcgcgcggag gggctggtga aggcatcggc gtggatggag
     1441 acaaatacgt ccgcccgccg tgcgcgccct ttggcgacac gcacgcccaa tgggatgaac
     1501 acgtcttcgt tgcgcgtcat aaagacgttg taacccaagg cttccaattg ttttttggtt
     1561 tcgcgggcga tggagaggac gacgtgtttt tcctgcaaac cgcccgggct gacggcgccg
     1621 gggtcttcgc cgccgtgtcc ggggtcgatc ataatgacgg gtctgcgccc gtttttgccg
     1681 cgttcggttt gtgcgggagt gttttgagcg aggtcggctt caggggagcc gcgacgggtt
     1741 ttgttcgggt tgccgttaag cagcgccatc atcggatcgt cggtatccat cccttgcgga
     1801 tagagatcga cgacgaggcg gtttttaaag ccgccgacgg gggggaggga aaaaacttgc
     1861 gcgtaggaag gctgtttcaa atcgatgacg aggcggacgg tgtcgggcgt gttctgaccc
     1921 gcgcgtatat tgcggataaa ggggtcgtcc gccgccactt tttgggacag tccgtgcaat
     1981 acggtattga tgtttgcgtt ttgtatgtcg acaaccagcc tgccggggtt gtcgagcgcg
     2041 aagtgctggt atttgagcgc ggttgtgctt tccagcgtca ggcgggtgta ggtgtgcgac
     2101 ggccatatcc gtgcggcggt gaattgtggt gcgcgcacgg ttttggcaac ggcggatgcg
     2161 atggggctta gggcgaacag tgtgccggcg gtgcggcgga tgatttgtct tcgtgtcagt
     2221 ttggtcatag cggcaggctt tcgcgtcctc gttcggtatg ggcggtcagc aggcattttc
     2281 tgccgccgcc gtcgtgtgtc aatgttgcgg tgatgtcggc aggcggggta aattcgcctc
     2341 cctgttgcgg ccattcgatc aggcagacgc tgtttgcggc aaacagttcg tcaagccccg
     2401 cgtcttccca ttcttcgggg aacgagaagc ggtagaggtc gaaatggtgc agggtgaagc
     2461 gttccagcgg ataagattcg acgatggcgt aggtcgggct tttgactgcg ccctgatgac
     2521 ccaatccgcg caggatgccg cgtgtcagcg tggttttgcc cgcacccaaa tccccttcga
     2581 gataaatgac cagcggtgcg tttaaacggg aagaccacgc cgcacccaaa tcgagtgtgg
     2641 cggcttcgtc ggcaaggaat cgggaaacgg gaggaaaatc aggcataggg gctttacaac
     2701 ttttatgtgg cggatcggag gggcaggcgg tcggcaaacc gtgcgcccgg cggcattgcc
     2761 gtacattata acagccaaat ccgcatcctg ctttcagacg gcaacggctg tcaagaaaaa
     2821 gcggcgcgtg tacaatacgc ggattgtatg tttaggacag attggaaaaa ggatggaaaa
     2881 tatcggcagg cagcgaccca tcggcgtttt tgactcggga atcggcggtt tgaccaatgt
     2941 gcgcgcactg atggagcggc tgccgatgga gaacatcatt tatttcggcg acacggcgcg
     3001 cgtgccttac gggacgaaat ctaaggcgac catcgaaaat ttctcgatgc agattgtcga
     3061 ttttctgctg gaacacgatg ttaaggcgat ggttatcgca tgcaatacga ttgcggcggt
     3121 ggcggggcag aaaatccgtc aaaagacggg caatatgccg gttttggacg tgatttccgc
     3181 cggcgcgaaa gccgcgctgg caacgacgcg caacaataaa atcggcatta tcgccaccaa
     3241 taccacagtc aacagcaatg cttatgcgcg cgccatccac agggacaacc ccgacacgct
     3301 cgtccgcacg caggccgcgc cgctgctcgt ccctttggtg gaagagggct ggttggaaca
     3361 cgaagttacc cgcctgaccg tatgcgaata cctcaaacca ttgcttgcag acggcatcga
     3421 tacgctggtg ttgggctgca cgcactttcc cttgctcaag cccttaatcg gcagggaggc
     3481 gcacaatgtc gcgttggttg attctgcaat tacgacggct gaagaaaccg cgcgcgtcct
     3541 tgcgcaggag ggattactca ataccgacaa caacaatccc gactatcgtt tttacgtcag
     3601 cgatattcct ttgaaattca gaaccatcgg cgagcgtttt ctgggcagga cgatggagca
     3661 gattgaaatg gtgtctttgg gttagcggca ggtacggacg gaaaccgtcc ccgtccgcgc
     3721 catccgcata cgcacccgca acgccctgcc gtattaaacg ccccgtcgcc gcaaatgccg
     3781 tctgaaaggc ttcagacggc attttttaca caattcccac cgtttcccat ccttctcgat
     3841 acactgtaat ctc
//
LOCUS       DAWHSK010000194         3833 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.194, whole genome shotgun sequence.
ACCESSION   DAWHSK010000194 DAWHSK010000000
VERSION     DAWHSK010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3833
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.194"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(69..263)
                     /locus_tag="WIC03_001711"
     CDS             complement(69..263)
                     /locus_tag="WIC03_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HEZ3884180.1"
                     /translation="MKHKLASWWKTIKLTANLMAGVPDYENYVAQQRKHNPNAPVMTK
                     LQFQDYCRKRRCGANGGRCC"
     gene            complement(253..2334)
                     /locus_tag="WIC03_001712"
     CDS             complement(253..2334)
                     /locus_tag="WIC03_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HEZ3884181.1"
                     /translation="MKSLKTFLIWGIVVLVGLASFTTLALSRGEQVSAVWMVTAAISV
                     YCIAYRFYSLYIANRVMRLDPNRLTPAERHNDGLDYVPTHKGVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGTLWIIFGVVFAGAVQDMMVLFVSMRRDGKSLGDIVKQELGTVPG
                     VIASIGILMIMVIIMAVLALIVVKALVHSPWGTFTIAATMPIALFMGIYTRYIRPGKI
                     GEISIVGFILLMLAVIYGEDVAKSSIGHWFDLDGIQLTWAIMIYGFVASVLPVWLLLT
                     PRDYLSTFLKIGTIAALALGIVIVNPTLQMPAVTHFIDGSGPVFSGALFPFLFITIAC
                     GAVSGFHALISSGTTPKMLENETHVRMIGYGGMLMESFVAIMALAAAASLDPGVYFAM
                     NSPAALIGTDANTAAEVITTKLQFPVDAATLLHTAKEVGENTILSRAGGAPTLAVGMA
                     HIMSRLIPGEAMMAFWYHFALLFEALFILTAVDAGTRVARFMIQDLGSIFYKPFGNTD
                     SIPANLIATFFAVALWGYFLYTGVTDPLGGINSLWPLFGIANQMLAGVALIMCAVVLI
                     KMKRDRYVWVVLVPAVGVLFVTCYAGLQKLFHSDPRISFLAHAGKYSDALAKNEVLAP
                     AKDIGEMAQIIFNDKINAGLTILFLSVVVIVAAYGLRTALKARKVGWPTAKEIPAVYR
                     DGKQPEAQSEA"
     gene            2810..3190
                     /locus_tag="WIC03_001713"
     CDS             2810..3190
                     /locus_tag="WIC03_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HEZ3884182.1"
                     /translation="MNIRFFALTVPVLSLAACAVPEAYDDGRRGYMPPVQNQAGPNDF
                     RAFSCENGLSVRVRRLDGGKIALRLDGRRAVLSSDVAASGERYTAEHGLFGNGTEWHQ
                     KGGEAFFGFTDAYGNSVETSCRAR"
     gene            complement(3323..3816)
                     /locus_tag="WIC03_001714"
                     /pseudo
     CDS             complement(3323..3816)
                     /locus_tag="WIC03_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713843.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CdiI family contact-dependent growth inhibition
                     immunity protein"
BASE COUNT          902 a          958 c         1088 g          885 t
ORIGIN      
        1 tgttaatcca ctatattata aaaattccgt ctgaagtggc gttcagacgg aatttgtttc
       61 aagcaggctt aacagcagcg tccgccgttt gcgccgcaac ggcgcttgcg gcagtagtct
      121 tgaaattgca gcttggtcat cacgggggcg ttgggattat gtttgcgctg ctgtgcaacg
      181 tagttttcat aatcaggcac gcctgccatc aagtttgccg tcagcttgat ggttttccac
      241 caagacgcga gcttatgctt cactttgtgc ctccggctgc ttgccgtcgc ggtacaccgc
      301 cgggatttct ttggcggtcg gccagccgac tttgcgtgct ttgagggcgg tacgcaaacc
      361 gtacgcggcg acaatcacga caaccgacaa gaagaggatg gtcagaccgg cattaatctt
      421 gtcgttgaag atgatttgcg ccatttcgcc gatgtctttg gcaggcgcaa ggacttcgtt
      481 tttagccaat gcgtcgctgt atttgccggc gtgggcaagg aagctgatgc gcgggtcgct
      541 gtggaacagt ttttgcaggc cggcgtagca ggttacgaac agtacgccga cggcgggaac
      601 gagtaccacc caaacataac ggtcgcgttt catcttaatc agcaccacgg cgcacataat
      661 caaggccacg cctgccagca tttggttggc gatgccgaac aaaggccaga gcgagttgat
      721 gccgcccagc gggtcggtta cgccggtgta gaggaagtag ccccacaatg ccacggcgaa
      781 gaaggtcgca atcaggttgg cggggatgga gtcggtgttg ccgaaaggtt tgtagaagat
      841 gctgcccaag tcttgaatca taaaacgtgc gacgcgcgta ccggcatcga cggcggtcag
      901 gatgaacaag gcttcaaaca atagggcgaa gtgataccag aacgccatca tcgcctcgcc
      961 cggaatcagg cggctcataa tgtgcgccat accgactgcg agggttggcg caccgcctgc
     1021 acgggaaagg atggtgtttt cgcccacttc cttagcggta tgcaacaggg ttgccgcatc
     1081 gacagggaat tgcagcttgg tggtaatcac ttcggcggcg gtgttggcat ccgtaccgat
     1141 cagggcggca gggctgttca tggcgaagta cacgccggga tcaagcgatg cggcggcggc
     1201 aagcgccata atggctacga aactttccat caacataccg ccgtaaccaa tcatacggac
     1261 gtgggtttcg ttttccagca ttttcggcgt ggtgccggag gaaatcagcg cgtggaagcc
     1321 cgaaaccgca ccgcaggcga tggtgatgaa gaggaacggg aacaatgcgc ctgagaatac
     1381 cggacccgaa ccgtcgataa agtgggttac ggcaggcatt tgcaaagtgg gattgacgat
     1441 gacgatgccc aaagccaagg ccgcaatcgt accgattttc aggaaggtgg agagatagtc
     1501 gcgcggagtg agcagcaacc atacgggcaa tacggaggcg acaaagccgt aaatcataat
     1561 cgcccaagtg agctggatgc cgtcaaggtc gaaccaatgt ccgatggaac ttttcgccac
     1621 atcttcgccg taaattaccg ccagcatcag caaaataaag ccgacgatgg aaatctcgcc
     1681 gattttgccc ggacggatat agcgcgtgta aatacccata aacagggcaa tcggcatagt
     1741 ggcggcgatg gtaaacgtac cccaagggct gtgaacaagt gcttttacga caatcaacgc
     1801 caacaccgcc ataatgatga ccataatcat caaaataccg atggaggcaa tcacgccggg
     1861 gacagtgccg agttcctgtt tcacaatatc gcccaaagac ttaccgtcac ggcgcataga
     1921 aacaaacagt accatcatat cctgtaccgc gccggcaaaa accacgccga agataatcca
     1981 caaagtaccg ggcaggtaac ccatttgcgc agccagtaca gggccgacca aagggcccgc
     2041 gccggcaatc gcggcaaagt ggtgtccgaa caatacgccc ttgtgcgtcg gaacgtagtc
     2101 caagccgtcg ttgtggcgtt ctgccggagt caggcggtta ggatcgagcc gcattacgcg
     2161 gttggcgatg tagaggctgt aaaaacggta ggcgatgcag taaacggata tggcggcggt
     2221 aaccatccat accgcgctga cctgttcgcc tcggctgaga gccagagtgg taaaggatgc
     2281 taagccgacc agtaccacta tgccccaaat gaggaaggtt ttgagtgatt tcatcgaata
     2341 aatccttatc tcacactgtc ggaatatgcc tgaacggcgg gaaacggatg accgcctgcc
     2401 ggcacgggca caaaccggag tctgaatgat tgacgaagta tgaaacagtg cgcttgtcgg
     2461 gatttgtgcc ttatgcccgc atcaaacagt gcagggatgc gggcgcacaa aacgttaaac
     2521 gccgaatagg attttaacgc aaattagcac actgatagcg gtttttactt ggaatttgga
     2581 aaaaatttac attccttcgg gcggacaggc gggtttggat ggcatcgtca ggcaaaaggc
     2641 ggcgccggaa gacgggtaaa gaaggggcgc gcaatccgga ttattgattc atcgcgggta
     2701 aattccggtt atcgggcttg tgtgtttgcg cgtccgttta tagtatggcg ttgccgcagc
     2761 ttggaatcag ggcggttgtt tcatatctta tttttattgg ggagctttta tgaatatcag
     2821 gtttttcgcg ctgaccgtac cggttttgtc tttggcggcc tgtgccgtgc cggaggcgta
     2881 tgatgacggc agacgcgggt atatgccgcc tgttcaaaac caagccggcc cgaacgattt
     2941 tcgggcgttt tcatgcgaga acggtttgtc tgtgcgcgtc cgccgtttgg acggtggcaa
     3001 aatcgcgttg cggctggacg gcaggcgtgc cgtcctctct tccgacgttg ccgcatccgg
     3061 cgaacgctat accgccgaac acggtttgtt tggaaacgga accgagtggc accagaaagg
     3121 cggcgaagcc tttttcggct ttaccgatgc ctacggcaat tcggtcgaaa cttcctgccg
     3181 cgcccgttaa acggggcagc ctgtttgaac cgccgtccgg atttttcggg cggttttttg
     3241 tgtcggattt gttttggctg tcggctggtt tcggcggttt tcgggcgtgg cggattattt
     3301 ttcgtttcgt tccgggttta tcctatcggc agcggctcaa tgccaacttt aaacctgctc
     3361 cgatttcttc agggctgtta tccaatgata aaatgacatc gtctgcatca atggcatccc
     3421 acgcttccag cttgacatgg cggctcgggc tgattttcag gcagccgttg tgcagccaaa
     3481 tatccccgct catcatgttt ttaaataggg cgcgtctggt tttatagccc aagttcccgc
     3541 atagtttggc aacccaatcc tcatagcgtt gccgaaattt ttcggtatca aaaaaatctt
     3601 ggtcttccga actgtcataa acgaaagtcc tgctgtttgc caacgcttgc aagaccgtcg
     3661 tgcctaaagt ttcattgtcg gtatccaatg gcaggatatg gggggatata ggtggtctat
     3721 tgccgttaac cccaaacctg aacatgtttg aacaatcaga gcttcttcat tagcataaaa
     3781 aactgcccaa taattttgat cctgtttaaa aattattatt tgatctccgt aat
//
LOCUS       DAWHSK010000195         3809 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.195, whole genome shotgun sequence.
ACCESSION   DAWHSK010000195 DAWHSK010000000
VERSION     DAWHSK010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3809
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.195"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            215..1732
                     /locus_tag="WIC03_001715"
     CDS             215..1732
                     /locus_tag="WIC03_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ3884183.1"
                     /translation="MLYFRYGFLVVWCAAGVSAAYGADAPAILDDKALLQVQRSVSDK
                     WAESDWKVENDAPRVVDGDFLLAHPKMLEHSLRDALNGNQADLIASLADLYAKLPDYD
                     AVLYGRAQALLAKLAGRPAEAVARYRKLHGENAADERILLDLAAAEFDDFRLKSAERH
                     FAEAAKLDLPAPVLENVGRFRKKTEGLTGWRFSGGISPAVNRNANNAAPQYCRQNGGR
                     QICSVSRAERAAGLNYEIEAEKLTALADNHYLLFRSNIGGTSYYFSKKSAYDDGFGRA
                     YLGWQYKNARQTAGILPFYQVQLSGSDGFDAKTKRVNNRRLSPYMLAHGVGVQLSHTY
                     RPNPGWQFSVALEHYRQRYREQDRAEYNNGRQDGFYVSSAKRLGESATVFGGWQFVRF
                     VPKRETVGGAVNNAAYRRNGVYAGWAQEWRQLGGLNSRVSASYARRNYKGVAAFSTEA
                     QRNREWNVSLALSHDKLSYKGIVPALNYRFGRTESNVPYAKRRNSEVFVSADWRF"
     gene            complement(1793..3574)
                     /locus_tag="WIC03_001716"
     CDS             complement(1793..3574)
                     /locus_tag="WIC03_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase component I
                     family protein/aminotransferase class IV"
                     /protein_id="HEZ3884184.1"
                     /translation="MPYFALFDDAVSGRAKRYQNHVESRFFRPEELDALDGALQSGWQ
                     KGLHAVLFADYGFGLPLTGVESERGGNLALHWFADCADTDAASWLARHSDGLPAGIST
                     PQSSVSEADYLDHIRQIHEAIRRGDTYQINYTTRLHLQAYGNPVKLYQRLRQPVPYAV
                     LSHLPDAQGQSAWTLCFSPELFLKIGSDGTISTEPMKGTAPILGDGQDERRAAELQAD
                     PKNRAENVMIVDLLRNDLGKIAQTGTVCVPEPFKVSRFGSVWQMTSTIQAQALPHTSF
                     ADILRAAFPCGSITGAPKKMSMQIIESLEAEARGLYTGSIGYLNPCSGGLGFEGTFNV
                     VIRTLSLTPLSDGIYQGVYGVGSGIVIDSDPAAEYRECGWKARFLNELRPDFGIFETL
                     RVENRQCALLDRHLCRLKTSAQALNLPLPDGCENQIKQYIADLPDGAFRVKALLASDG
                     ISLSRAVLNHLADKQRVIISPTILPAQNYLRRFKTTHRALFDQAWQTAETQGAFDSLF
                     FNSDGILLEGGRSNVFVKHRGQWLTPSLDLDILNGIMRQAVLDEPQKYLQTNQVIETH
                     ITQKTLQEAEEIRLSNALRGVFAAALA"
BASE COUNT          786 a          853 c         1256 g          914 t
ORIGIN      
        1 ttccgtacta tctgtactgt ctgcggcttc gtcgtcttgt cctgattttt gttaatccac
       61 tataaatgtg aaatccgccc tttgaaaatc gggcggcggt tttttgcctg cccgctttca
      121 gacggcaaag ccggttttca cgggtttctg cctgtttttc ggatggtttg acgtgcttcg
      181 gcggcgtgtt tgccggaaag gtaaatgaca gggtatgttg tatttcagat acggcttttt
      241 ggttgtttgg tgtgcggcag gtgtttctgc cgcctatggg gcggatgcgc ccgcgatttt
      301 ggatgacaag gcattgttgc aggtgcagcg gtcggtgtcg gataagtggg cggaatcaga
      361 ttggaaagtt gaaaatgatg ccccgcgcgt ggttgacggg gattttttgt tggcgcaccc
      421 gaaaatgttg gaacatagtt tgcgcgacgc gctcaacggc aatcaggcgg atttaatcgc
      481 ttcgttggcg gatttgtatg ccaagctgcc ggattatgat gcagttttgt acggcagggc
      541 gcaggctttg ctggcgaaat tggcgggaag gccggcggag gcggtggcgc ggtatcggaa
      601 gctgcacggg gaaaatgcgg cagacgagcg gattttgctg gatttggcgg cggcggagtt
      661 tgacgatttc cggctgaagt cggcagaaag gcattttgcg gaggcggcaa aattggattt
      721 gccggcgccg gttttggaaa atgtggggcg ttttcggaaa aaaacggagg ggctgacggg
      781 ctggcgtttt tcgggcggca tcagcccggc ggtcaataga aatgccaata atgccgcgcc
      841 gcagtattgc cggcaaaacg gaggccggca gatatgcagt gtcagccggg cggagcgggc
      901 ggcagggttg aattatgaaa tcgaggcgga aaaactgacg gcgttggcag ataatcatta
      961 tttgttgttc cgttccaata tcggcggcac gagctattat ttcagtaaaa aatcagctta
     1021 tgatgacggg ttcggtaggg cgtatttggg ttggcagtat aaaaatgcgc ggcagacggc
     1081 ggggattttg ccgttttatc aggtgcagtt gtcgggcagc gacggctttg atgcgaaaac
     1141 aaaacgggta aacaaccgcc gcctgtcgcc gtatatgctg gcgcacggag tcggcgtgca
     1201 gctgtcccat acttaccgcc caaacccggg atggcaattt tcggtcgcgc tggaacatta
     1261 ccgccaacgc taccgcgaac aggatagggc ggaatacaat aacggccggc aggacgggtt
     1321 ttatgtttcg tcggcaaaac gtttgggcga atcggcaact gtgttcggcg gctggcagtt
     1381 tgtgcggttt gtgccgaaac gcgaaacggt gggcggcgcg gtcaataatg ccgcctaccg
     1441 gcgcaacggt gtttatgccg gttgggcgca ggagtggcgg cagttgggcg gtttgaacag
     1501 tcgggtttcc gcgtcttatg cccgccgcaa ctataagggc gttgcggctt tctcgacaga
     1561 ggcgcaacgc aaccgcgaat ggaatgtctc gctggctttg agccacgaca agttgtcgta
     1621 caaaggtatc gtgcccgcgt tgaattatcg tttcggcagg acggaaagta atgtgccgta
     1681 tgcgaaacgc cgcaacagcg aggtgtttgt gtcggcggat tggcggtttt gaatggtggg
     1741 ataatgccgt ccgaactttg cggcagcagg ttcggacggt atttttgcgc gttcaggcaa
     1801 gggcggcggc aaatacgccg cgcagggcgt tggagaggcg gatttcttcg gcttcttgca
     1861 gtgttttttg tgtgatgtgt gtttcgatta cttgatttgt ttgcaaatat ttttgcggtt
     1921 cgtccaacac ggcttggcgc attatgccgt ttaaaatgtc taaatctaaa gagggcgtga
     1981 gccattgtcc gcgatgtttg acgaacacgt tgcttctgcc gccttcgagc aggatgccgt
     2041 ctgaattgaa aaacaggctg tcgaacgcgc cttgtgtttc ggcggtttgc cacgcttggt
     2101 cgaagagggc gcggtgtgtg gttttgaagc ggcgcaggta gttttgtgcg ggcaggatgg
     2161 tcggcgaaat gatgacgcgc tgtttgtcag ccaaatggtt taaaacggcg cgggacaggc
     2221 tgatgccgtc tgaagcgagc agggctttga cgcggaacgc gccgtcgggc aagtcggcaa
     2281 tgtattgttt gatttgattt tcgcaaccgt cgggcagggg caggttgagg gcttgggcgg
     2341 aggttttcag acggcatagg tggcggtcga gcagggcgca ttgcctgttt tccacgcgca
     2401 gggtttcaaa aatgccgaag tcggggcgca attcgttgag gaaacgggct ttccagccgc
     2461 attcccgata ttcggcggca gggtcgctgt cgatgacgat gccggaaccg acaccgtaca
     2521 cgccttgata aatgccgtct gaaagcggcg tgagcgacaa ggtgcggata acgacgttga
     2581 acgtgccttc aaaccccaag ccgccggaac acgggttcaa atagccgatg ctgcccgtat
     2641 aaagtccgcg cgcttcggct tcgagcgatt cgataatctg catactcatt tttttgggcg
     2701 cgccggtgat gctgccgcag gggaaggcgg cgcggaggat gtcggcgaac gaggtgtgcg
     2761 gcaaggcttg ggcttggatg gtgctggtca tctgccaaac gctgccgaaa cgcgatactt
     2821 taaacggttc gggtacgcat actgtgccgg tttgggcgat tttgccgagg tcgttacgca
     2881 gcaaatcgac aatcatcacg ttttcggcgc ggtttttcgg gtctgcttgc aactcggcgg
     2941 cgcggcgttc gtcttgtccg tcgcccaaaa tcggcgcggt gcctttcatc ggttcggtgc
     3001 tgatggtgcc gtccgaaccg attttgagga agagttcggg cgagaaacac agcgtccacg
     3061 cggattgccc ttgtgcatcg ggcaggtggg acaaaacggc ataggggacg ggctggcgca
     3121 ggcgttggta aagtttgacg ggattgccgt aggcttgcag gtgcaggcgg gtagtgtagt
     3181 tgatttgata ggtgtcgccg cgtcggatgg cttcgtggat ttggcggata tggtcgaggt
     3241 aatcggcttc ggatacggag gattgcggcg tggaaatgcc ggcggggagg ccgtctgagt
     3301 gtcgggcaag ccagcttgcg gcatcggtgt cggcgcagtc ggcaaaccag tgcagggcaa
     3361 ggttgccgcc gcgttcggac tcgacccccg tcagcggcaa accgaatccg tagtctgcaa
     3421 acaacacggc atgcagccct ttttgccagc ccgattgcag cgcgccgtcc aaagcatcga
     3481 gttcttcggg acggaaaaaa cggctttcca catgattttg atagcgtttt gcgcggccgc
     3541 ttacggcatc gtcaaacagg gcgaaataag gcatggcaat tcggggcaaa tgttttgatt
     3601 atacgctctt tttacacata ttttcagacg attcggcaaa tatcggcaaa atgtaatttt
     3661 atgtagagaa agtggggggc gaaggtgtaa aattgtgaaa acaaattccg atttccaacc
     3721 tgaaatacaa taaggagacc tttatgacag accaccagct gcaaccgttt gaaaacgtag
     3781 aattaggcga aaagcaagat cagctccaa
//
LOCUS       DAWHSK010000196         3701 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.196, whole genome shotgun sequence.
ACCESSION   DAWHSK010000196 DAWHSK010000000
VERSION     DAWHSK010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3701
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.196"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..294)
                     /locus_tag="WIC03_001717"
     CDS             complement(<1..294)
                     /locus_tag="WIC03_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884185.1"
                     /translation="MNLVKLLANNWQPIAIIALVGTGLAVSHHQGYKAAFAKQQAVID
                     KMEHDKAQALLLSAQNYARELELARAEAKKYEVKAHAVGMALAKKQAEVSRLKT"
     gene            complement(266..502)
                     /locus_tag="WIC03_001718"
     CDS             complement(266..502)
                     /locus_tag="WIC03_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884186.1"
                     /translation="MRILDIFKNPATGNVSHSKLWANVACAAGTFKFVMLPDPSAEIW
                     AVYLGIVGGYAVARSFVSVKRQEVENESRETAGE"
     gene            complement(505..669)
                     /locus_tag="WIC03_001719"
     CDS             complement(505..669)
                     /locus_tag="WIC03_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884187.1"
                     /translation="MGQVEFYEKMIELWSSKSREASERADLAAFEFAEGELANYREML
                     KRHLQTKSVE"
     gene            complement(672..878)
                     /locus_tag="WIC03_001720"
     CDS             complement(672..878)
                     /locus_tag="WIC03_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884188.1"
                     /translation="MNIIGKLKEAASYFLTKLIGENPSNEQVNRALIQMPNVRPIHTY
                     PRPNLRNSGVAAAKRAARKRKNRR"
     gene            complement(875..1420)
                     /locus_tag="WIC03_001721"
     CDS             complement(875..1420)
                     /locus_tag="WIC03_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEZ3884189.1"
                     /translation="MGKTVTLTAGHSNTDPGAVNGSDREADLAQDMRNIVASILRNDY
                     GLTVRTDGEGKGNMPLRDAVKLIRGSDVAIEFHTNAAVSKAATGIEALSTVKNKRWCQ
                     VLSKAVAKKTGWKLRGEDGFKPDNAGQHSRLAYAQAGGIVFEPFFISNDTDLALFKTT
                     KWGICRAIADAIAMELGAARV"
     gene            complement(1577..2005)
                     /locus_tag="WIC03_001722"
     CDS             complement(1577..2005)
                     /locus_tag="WIC03_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mor transcription activator family protein"
                     /protein_id="HEZ3884190.1"
                     /translation="MAFEKVEHLLPDTVLDIVDVIGLAATEQLVKAIGGARFKFGKGK
                     VDTERLAILVEAIGEVKTHELLQVYGGEELYVPRCGKALIQLRNHRFYQEFVKLRDID
                     KESGLMAMTKLCPKYGISSRTGYTIINEMSRPAAQQAALF"
     gene            complement(1986..2420)
                     /locus_tag="WIC03_001723"
     CDS             complement(1986..2420)
                     /locus_tag="WIC03_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HEZ3884191.1"
                     /translation="MRRALIAKIKIAQKELGLDDGTYRAVLERVTGKRSCADMDVSEL
                     ESVVADMRSHGFKPKAKGNPHGKPHLRRTSSAAMLDKVEALLTVGGKHWNYAHAMARR
                     MFGKDKVEYLDDTQLHKLVAALQIAENRKTEKAGGDDGVRKS"
     gene            complement(2577..3533)
                     /locus_tag="WIC03_001724"
     CDS             complement(2577..3533)
                     /locus_tag="WIC03_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEZ3884192.1"
                     /translation="MLIHYIDIAKRNFVIAVSSLSKTKTETNNPKGIAHTIEYLKKHK
                     VALVVTESTGGLEIPAAKAIRRAGIAVIIANPRQTHQFAQSQPLTKTDAKDAKMLAFF
                     AQMMTQKEDWQTMPYQPPTEAEEVLEALVNRRNQSADMRTAEKNRLHQVHETQVGSVK
                     QLIAHFDRLIDELDKQIDDHTHTHFDGKAQVAEQIKGIGSITTATLMAMLPELGRLSH
                     KRIASLAGIAPHPRESEETKFKSRCFGGRSAVRKALYMATVVATRFKPLIRDFYQRLP
                     SEGKPYKVAVTAYMRKLLTISNARMRDYFAENDTAENGIRTA"
BASE COUNT          836 a         1042 c          847 g          976 t
ORIGIN      
        1 cgttttcaga cggctgactt ccgcctgttt tttcgccaaa gccatgccga cagcgtgcgc
       61 cttgacttca tattttttcg cttccgcgcg tgccagttcc agttcgcgcg catagttttg
      121 agccgacaac agcagggctt gcgccttatc gtgctccatc ttgtcgatga ccgcctgctg
      181 cttcgcaaag gctgccttgt agccttgatg gtgtgacacc gccaaacccg tgccgacaag
      241 cgcgatgatg gcaatcggct gccagttatt cgccagcagt ttcacgagat tcattctcga
      301 cctcctgacg tttgacgctg acaaacgagc gcgccacggc atagccgccg acaatgccca
      361 aatacaccgc ccaaatctcc gccgacgggt cgggcaacat cacaaattta aacgtccccg
      421 ccgcgcaggc gacgtttgcc cacagcttcg agtgcgacac attgcctgtc gccgggtttt
      481 taaaaatatc caaaatacgc attgctattc cacacttttg gtttgcaggt gccgtttcag
      541 catttcccga taattggcca gttcgccctc cgcaaattca aacgccgcca aatcagcccg
      601 ttcgcttgcc tcacggcttt tgctcgacca cagctcaatc atcttttcgt aaaactcaac
      661 ctgtcccatg attaacgacg attcttgcgt ttgcgcgccg cgcgtttggc tgccgccacg
      721 cctgagtttc ttaaatttgg gcgtggatag gtgtgtatcg gacgaacatt tggcatctgt
      781 ataagtgcgc ggtttacctg ctcattacta ggattttctc caatcaattt tgtaagaaaa
      841 taggaagcag cttctttcaa tttaccaata atattcatac tcttgccgcc cccaattcca
      901 tcgcaatcgc gtccgcaatc gcgcggcaga tgccccattt agtagtctta aacaaggcta
      961 aatcagtgtc gttgctgata aaaaacggct caaacacaat gccgccggct tgtgcataag
     1021 ccaggcgcga atgctggccc gcattgtcgg gtttaaagcc gtcttcgccg cgcagtttcc
     1081 agccggtttt cttggcaaca gctttgctca acacctgaca ccagcgtttg tttttaacgg
     1141 tactcaaggc ttcgatgcct gtcgccgctt tgctgacggc agcgttggtg tgaaactcaa
     1201 tcgccacatc cgagccgcga atcagcttga ccgcatcgcg caagggcata ttgcctttgc
     1261 cttcgccgtc ggtgcgtacg gtcaggccgt aatcgttacg caggattgaa gccacgatgt
     1321 tgcgcatatc ctgcgccaag tccgcctcac ggtcgcttcc gttgaccgcg cccgggtcgg
     1381 tgttactgtg tccggcggtt aaagttacag ttttgcccat tcatcatctc caaaaaatat
     1441 tgattgcata acatttgaaa accccattaa accgtcttta acccatcgtc ccgaacggta
     1501 gatgtttcat cccctacaaa ccaaaaaaaa agaccgtctg aatacagacg gccaaagcct
     1561 ggtcacacat cactgcctaa aataaagctg cctgctgtgc cgcaggtcgg ctcatttcat
     1621 tgataatcgt atatcctgtt cgtgaagaga taccgtattt agggcatagc ttcgtcatcg
     1681 ccataagccc gctctcctta tcaatatcgc gcaatttgac aaactcctga taaaacctat
     1741 ggtttctcaa ctgtattaac gccttgccgc accgtgggac atacaattcc tcgccaccat
     1801 atacctgcaa cagctcatgt gttttcactt cgccgatggc ttcgaccaaa attgccaaac
     1861 gctcggtgtc caccttgccc ttaccaaatt taaaccgcgc cccgccaatc gccttgacca
     1921 gctgttccgt cgctgccagt ccgatgacat ccacaatgtc caacacggta tccggcaata
     1981 aatgttcaac tttttcgaac gccatcatcc ccacccgctt tttccgtttt cctgttttcc
     2041 gcaatctgca acgcagcaac cagtttatgt agctgcgtat cgtctaaata ttcgacctta
     2101 tccttaccaa acatccgccg cgccattgcg tgtgcatagt tccaatgttt gccgccgacg
     2161 gtcagcaggg cttcgacttt gtccaacatt gccgctgatg atgtccgacg cagatgcggt
     2221 ttgccgtgtg ggttaccttt tgctttaggc ttaaatccgt gcgaccgcat atcagcgaca
     2281 acagactcaa gttcggaaac atccatatcc gcacacgacc gcttgcccgt cacacgctcc
     2341 aacaccgcgc gataggtacc gtcgtccaag cccagctcct tttgagcaat cttaattttc
     2401 gcaatcaacg cccggcgcat ctcaaaccca taaaacacaa tatattgact aattaacgca
     2461 tattatacca ataaaataca atatatagta ttaagccgat gttttttgcg aaacggacag
     2521 acataaaaaa gcaactgtat ttttcacccc gtcgggcaaa aataccaaaa ctcaaatcaa
     2581 gccgtccgga taccgttttc ggcggtatcg ttttcggcaa aataatcacg catccgggca
     2641 ttcgatatcg tcagcagttt gcgcatatat gccgtaacgg caaccttata cggcttaccc
     2701 tcggacggca ggcgttggta gaaatcccga ataagcggtt taaaacgtgt cgctaccacg
     2761 gtagccatat acagcgcctt acgtaccgca gaccttccgc cgaagcagcg gcttttgaat
     2821 ttggtttcct cgctctccct cgggtgcggg gcaatgccgg ccaaactcgc tatccgtttg
     2881 tgcgacagcc gccccaactc aggcagcatc gccatcagcg tagccgtcgt tatcgaaccg
     2941 atgcctttga tttgctccgc cacttgggct ttgccgtcaa aatgcgtgtg ggtgtggtcg
     3001 tcgatttgtt tgtccaattc gtcaatcagc cggtcaaaat gggcaatcag ttgtttgacg
     3061 cttccgactt gcgtttcgtg aacctgatgc agacggtttt tctcggcagt ccgcatatcc
     3121 gccgattggt tgcggcggtt aaccaaggct tccaacactt cttccgcttc tgtgggcggt
     3181 tggtagggca tggtttgcca atcttctttc tgcgtcatca tctgtgcgaa gaaggcgagc
     3241 attttggcat ctttggcgtc ggttttggtc agcggctgcg attgggcaaa ctgatgcgtc
     3301 tgacgcgggt tggcgataat cacggctatg cctgctcggc ggatggcttt ggcggcgggg
     3361 atttcgagac cgccagtact ttccgtcacg acgagggcga ccttgtgttt tttaaggtat
     3421 tcgatagtat gggcgatacc tttggggttg ttggtttcgg ttttggtttt agacaaagac
     3481 gaaacggcga tgacgaagtt tcgtttggcg atgtcgatat agtgaattaa caaaaatcag
     3541 gacaaggcgg cgagccgcag acagtacgga tagtacggaa ccgactcact tggtgcttca
     3601 gcaccttaga gaatcgttct ctttgagcta aggcgaacca acgctgtact ggtttagatt
     3661 taattcacta tacctgcgta atggtattgg gtactcatca t
//
LOCUS       DAWHSK010000197         3689 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.197, whole genome shotgun sequence.
ACCESSION   DAWHSK010000197 DAWHSK010000000
VERSION     DAWHSK010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3689
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.197"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            328..1248
                     /gene="ribF"
                     /locus_tag="WIC03_001725"
     CDS             328..1248
                     /gene="ribF"
                     /locus_tag="WIC03_001725"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEZ3884193.1"
                     /translation="MRIRLGRHNAPDFPHGAAVTIGNFDGVHLGHKHILQKLRLEADT
                     RGLPVVTVVFEPQPKEFFALRTGRMPPCRISPLRTKLELLEGTGCVDAVWVLRFDQNF
                     SEISAQGFIDRLLRQTLNTRYLLVGDDFRFGAGREGCFELLVQQPDMQTERTPSVIVE
                     DIRTSSTAVRQALSDGNLAYAKKLLGHDYVLSGRVVHGRKLGRTLNAPTANIRLPRHR
                     YALGGVFVVEADGAFGTRRGVASFGFNPTVDSGCSQKLEVHLFDFQGDLYGQRLNVRF
                     LHKLRDEEKFDGMAELKRQIAADMEAAKCW"
     gene            1389..>3689
                     /gene="ileS"
                     /locus_tag="WIC03_001726"
     CDS             1389..>3689
                     /gene="ileS"
                     /locus_tag="WIC03_001726"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEZ3884194.1"
                     /translation="MTDYSKTVNLLESPFPMRGNLAKREPAWLKSWYEQKRYQKLREI
                     AKGRPKFILHDGPPYANGDIHIGHAVNKILKDIIIRSKTQAGFDAPYVPGWDCHGLPI
                     EVMVEKLHGKDMPKARFRELCREYAAEQVARQKKDFIRLGVLGDWDHPYLTMDFKTEA
                     DTVRMLGEIYKSGYLYRGAKPVQFCLDCGSSLAEAEVEYKDKVSPAIDVGYPFKDTAA
                     LATAFGLAGIEGKAFAVIWTTTPWTLPASQAVSAGADVVYQLIDTPTGKLVLAKDLAE
                     DALKRYGFSDGIAILAETTGDKLENLHMNHPFLERDIPMLNGEHVTTDAGTGLVHTAP
                     AHGLEDYAVCNKYGIELYNPVNAEGKYISETPRVAGMRVWEANPVILQWLEETGNLLA
                     SSKIEHSYAHCWRHKTPLIYRATGQWFVGMDKAGADGKTLRDKAIKAVDDTEFFPSWG
                     RARLEAMIEGRPDWVVSRQRYWGTPMTFFVHKETGELHPNSAELLEKVAQRIEEKGIE
                     AWFSLDKSELLSAEDCEHYDKLPDTMDVWFDSGSTHYSVVKQREELEWPADLYLEGSD
                     QHRGWFQSSMLTGCASSMGRAPYKQLLTHGFVVDGEGKKMSKSIGNVVAPQEVYNEFG
                     ADILRLWAASTDYSGELAISKEILKRVTESYRRIRNTLSFLFANLSDFNPIEDAVQQA
                     DMVEIDRYALVLARRLQERLAGDYYPRYAFHFAVKDIVSFCSEDLGAFYLDILKDRLY
                     TTKADSRARRSAQTALYHITRSLVLLI"
BASE COUNT          888 a         1021 c          970 g          810 t
ORIGIN      
        1 cggggtttga gcaactgttt ttatccgata agtttctgtg cggacaggtc cggattcccg
       61 cctgcgcggg aatgacggcg gagggttgtt tgtctcggtt tacctggtta aaaaagaacg
      121 attttcactg atgttgcatc aggtttgggg cgatgtttca acacatagca ccgcgcctgc
      181 tgcgcgcttt atgcgtttgg cgcgttcggc tgcgggaaat ttgcctactt ttcccgcgtc
      241 gggcgggcgt aacgggcggc acactgtcta taaaccgcaa taccgtttac aatgaccgcc
      301 tgtttcacca catacccgaa cataacaatg agaatcaggc tggggcggca caacgcgccc
      361 gactttccgc acggggccgc cgtaaccata ggcaattttg acggcgtaca cctcggacac
      421 aaacacatcc tccaaaaact ccgcctcgaa gccgatacgc gcggattacc cgtcgtgacc
      481 gtcgttttcg aaccccaacc caaagaattt ttcgcactcc gcaccggcag gatgccaccg
      541 tgtcggatca gccccctgcg caccaagctc gaattattgg aaggcacagg ctgtgtcgat
      601 gccgtctggg ttttgcgttt cgatcaaaat ttttccgaaa tatccgcgca agggtttatc
      661 gaccgcctgc tgcgtcaaac cttgaatacg cgttatttgc tcgtcggcga tgatttccgt
      721 ttcggtgcgg ggcgggaagg ctgttttgaa cttttggtac aacagcccga tatgcagacc
      781 gagcgtacgc cttccgtcat cgtcgaagac atccgcacca gcagtaccgc cgtgcgacaa
      841 gccctttcag acggcaacct tgcctatgcg aaaaaacttt tgggacacga ctacgtcttg
      901 agcggcaggg tggtgcacgg cagaaaactc ggacgcacct taaacgcccc gactgccaac
      961 atccgcctgc cccgccaccg ttatgcactc ggcggcgtgt tcgtcgtcga agcggacggc
     1021 gcattcggca cgcggcgcgg cgtggcgagc ttcggtttca atcccaccgt tgatagcggc
     1081 tgttctcaaa agcttgaagt ccacctgttc gactttcaag gcgacctgta cggacaacgg
     1141 ctgaacgtcc gcttcctgca caaactgcgc gatgaagaaa agtttgatgg tatggcagaa
     1201 ctgaaaaggc agatagcagc cgatatggaa gccgcaaagt gttggtagaa aaaccttata
     1261 caaaccatcc gattgggcta caatcaatct tttaactttt cagacggcat aggggtttcc
     1321 cgttgtgaaa tactgtttga ggcgcaatgc cgtctgaacc gaaatattgt aacaatagag
     1381 attaaaaaat gaccgattac agcaaaaccg tcaacctgct cgaaagcccg ttcccgatgc
     1441 gcggcaatct tgccaagcgc gagcctgcat ggctgaaaag ctggtacgag caaaaacgct
     1501 accaaaaact gcgcgaaatc gccaaaggcc gtccgaaatt catcctgcac gacggcccgc
     1561 cgtatgccaa cggcgacatc cacatcggtc atgccgtcaa taaaattctc aaagacatca
     1621 ttatccgcag caaaacccaa gccggttttg acgcgcctta tgtgccgggt tgggactgcc
     1681 acggtttgcc catcgaagtg atggtagaaa aactgcacgg caaagacatg cctaaagcgc
     1741 gtttccgtga attgtgccgc gaatatgccg ccgaacaagt tgcccgccag aaaaaagact
     1801 ttatccgctt gggcgtgttg ggcgactggg accatcctta cctgactatg gatttcaaaa
     1861 ccgaagccga taccgtgcgt atgctcggcg aaatctacaa atccggctat ctctaccggg
     1921 gtgcgaaacc ggttcaattc tgcttggact gcggttcttc gctggccgaa gcggaagtgg
     1981 aatacaaaga caaagtatcg cctgcgattg acgtgggcta tccgtttaaa gacaccgctg
     2041 cgcttgccac tgcattcggt ttggcaggta tcgaaggcaa agcgtttgcc gttatctgga
     2101 caaccacgcc ttggacgctg ccggcgagcc aagccgtgtc tgccggtgcg gacgtggtgt
     2161 atcaattaat cgatacgcct acaggcaaat tggtgctggc gaaagatttg gcagaagacg
     2221 cgctcaaacg ttacggtttt tcagacggca ttgctattct tgccgaaacc accggcgaca
     2281 agctggaaaa cctgcacatg aatcatccgt tcctcgagcg tgatattccc atgctcaacg
     2341 gcgaacacgt taccaccgac gccggtacgg gtttggtaca taccgcccct gcgcatggtt
     2401 tggaagacta cgctgtctgc aataaatatg gcatcgagct ttacaacccc gtcaacgccg
     2461 aaggcaaata catcagcgaa acgccgcgcg ttgccggtat gcgcgtttgg gaagccaatc
     2521 ccgttatcct gcaatggctg gaagaaaccg gcaacctttt ggcaagcagt aaaatcgaac
     2581 acagctacgc ccactgctgg cggcacaaaa cgccgctgat ttaccgcgcg accggtcaat
     2641 ggtttgtcgg tatggacaaa gccggtgccg acggcaaaac cctgcgcgac aaagccatca
     2701 aggccgtgga cgataccgaa ttcttcccgt cttggggtcg cgcgcgtttg gaagccatga
     2761 ttgaaggtcg tcctgactgg gtggtttcac gccaacgcta ttggggcacg ccgatgactt
     2821 tctttgttca caaagaaacg ggcgagctgc atccgaactc tgccgaactt ttggaaaaag
     2881 tcgcgcaacg cattgaagaa aaaggcatcg aggcttggtt ctccctcgat aaaagcgaat
     2941 tattaagcgc ggaagattgc gaacattacg acaaactccc cgatacgatg gacgtatggt
     3001 tcgattccgg ttcgacccat tattccgttg tgaaacaacg cgaagaattg gaatggccgg
     3061 cggatttgta tctcgaaggc agcgaccagc accggggctg gtttcaatcg tccatgctga
     3121 ccggctgcgc ctcatcaatg ggacgcgcac cgtataaaca gctgctgacc cacggttttg
     3181 ttgtagacgg cgaaggcaaa aaaatgtcga aatccatcgg caacgtcgtc gcgccgcaag
     3241 aagtctataa cgaattcggc gcggacatcc tgcgtctgtg ggcggcatct accgattaca
     3301 gcggcgaatt ggcgatttct aaagaaatcc ttaagcgcgt aaccgaaagc taccgccgta
     3361 tccgcaatac cttgagcttt ttgtttgcca acttgagcga ctttaatccg attgaagatg
     3421 ccgtgcaaca ggcggatatg gtggaaatcg accgctatgc cctggtgttg gcgcgtcggc
     3481 tgcaagagcg tctggcaggc gattactatc cgcgttatgc cttccatttt gccgtgaaag
     3541 acattgtttc tttctgctcg gaagacttgg gcgcgttcta cctcgatatc ctgaaagacc
     3601 gcctctacac caccaaagcc gacagccgcg cccgccgcag cgcgcaaacc gccctgtacc
     3661 acatcacgcg cagcctggtt ctcttgatt
//
LOCUS       DAWHSK010000198         3653 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.198, whole genome shotgun sequence.
ACCESSION   DAWHSK010000198 DAWHSK010000000
VERSION     DAWHSK010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3653
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.198"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(117..272)
                     /gene="rpmG"
                     /locus_tag="WIC03_001727"
     CDS             complement(117..272)
                     /gene="rpmG"
                     /locus_tag="WIC03_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEZ3884195.1"
                     /translation="MRDKIKLESSAGTGHFYTTTKNKRTMPGKLEIKKFDPVARKHVV
                     YKETKLK"
     gene            complement(304..537)
                     /gene="rpmB"
                     /locus_tag="WIC03_001728"
     CDS             complement(304..537)
                     /gene="rpmB"
                     /locus_tag="WIC03_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEZ3884196.1"
                     /translation="MARVCKVTGKRPMSGNNVSHANNKTKRRFLPNLQSRRFWVESEN
                     RWVRLRVSNAALRTIDKVGIDVVLADLRARGEA"
     gene            complement(787..2313)
                     /gene="farB"
                     /locus_tag="WIC03_001729"
     CDS             complement(787..2313)
                     /gene="farB"
                     /locus_tag="WIC03_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     permease subunit FarB"
                     /protein_id="HEZ3884197.1"
                     /translation="MDYPPLKGAALAWVTLSLGLAVFMEVLDTTIANVAVPVIAGNLG
                     AATTQGTWVITSFAVANAVSVPLTGFLAKRIGEVKLFTAAAVGFVITSWLCGIAPNLQ
                     SLVVFRILQGFIAGPLIPLSQSLLMASYPPAKRTLALALWAMTVVVAPVLGPILGGWI
                     SGNWHWGWIFFINIPIGIISAWITWKHLKCRETETVKMPTDYVGLTLMVVGIGALQMM
                     LDRGKELDWFASGEIITLGVVALVCLSYFIVWELGEKYPIVDLSLFKDRNFTVGIIAT
                     SLGFMVYMGTLTLLPLVLQTNLGYTSTWAGLAAAPVGILPVFLSPLIGRFGNKIDMRL
                     LVTASFLTFAFTFYWRTDFYADMDIGNVIWPQFWQGVGVAMFFLPLTTITLSHMKGGQ
                     IAAAGSLSNFLRVLMGGVGVSVVSTLWERREALHHTRFAEHITPYSATLHETAAHLSQ
                     HGVSDIQTAGIINNTITQQGFIIGSNEIFMAGSLLFIIMIPVIWLAKPPFHSSGGGGH
                     "
     gene            complement(2337..3491)
                     /gene="farA"
                     /locus_tag="WIC03_001730"
     CDS             complement(2337..3491)
                     /gene="farA"
                     /locus_tag="WIC03_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     adaptor subunit FarA"
                     /protein_id="HEZ3884198.1"
                     /translation="MDTHADETKLQNTQAKRKRRLTALTLLFALAAAAAGSAFFLWWQ
                     HEEETEDAYVAGRVVQVTPQKGGTVRKVLHDDTDAVKKGDVLAVLDDDNDVLAYERAK
                     NELIQAVRQNRQQNAATSQAGAQVALRRADLARAQDDLRRRSALAESGAVSAEELAHA
                     RAAVSQAQAAVKAALAEESSARAALGGQVSLREQPAVQTAIGRLKDAWLNLQRTQIRA
                     PADGQVAKRSVQVGQQVAAGAPLMAVVPLSDVWVDANFKETQLRHMKIGQPAELVSDL
                     YGKQIVYRGRVAGFSAGTGSAFSLIPAQNATGNWIKVVQRVPVRIVLNREDVDRHPLR
                     IGLSMTVKVDTSAAGAPVSKTPGAALPEMESTDWSEVDRTVDEILGQSAP"
BASE COUNT          908 a         1018 c          909 g          818 t
ORIGIN      
        1 tccttggcag acggtttgca gcagattcgc atacggcaga tgtttcaagc ggaaaagaaa
       61 cacgataaaa ataaaaaagc ctccgaacag tcggaggctt tgctttcaaa ctgaaattat
      121 ttcagtttgg tttctttgta cactacgtgt ttgcgggcaa ccggatcgaa ttttttaatt
      181 tccagtttgc cgggcatagt gcgtttgttt ttggtagtgg tgtagaagtg accagtacct
      241 gcactggatt ccagtttgat tttatcgcgc attgcagtaa tccttaatta aatagtgttt
      301 aaattaagct tcgccgcgag cacgcaaatc agccaatacg acatcaatgc ctactttgtc
      361 gatggtacgc agtgcagcgt tggaaacgcg caggcgaacc cagcggtttt cactttctac
      421 ccaaaaacga cgtgattgca agttgggcaa aaaacggcgt ttggttttgt tgttggcgtg
      481 cgatacgttg ttgccggaca tcgggcgttt gccggtcact ttgcaaactc gtgccataat
      541 cagtctccaa acttctaaaa tagtaaaacg cgatttatac cacgaaaaac agtgttagtt
      601 caagtgtttt aaagaaaaac ggctgttttg cgcggataat ccttccgatt gtcgcatctg
      661 cggaacgttt gcaggtgcga aacagatggg gcgcgattat agcggatttc gggaactgct
      721 gcattcaaaa tgccgtccga acatatttcc agtattttca gacggcattt caagttttca
      781 aatccctcaa tgtccaccgc cgccactgct gtggaacggc ggttttgcca gccatatgac
      841 gggtatcatg ataatgaata acaagctgcc cgccataaag atttcgttcg agccgataat
      901 aaaaccctgc tgggtaatgg tattgttgat aatacctgcg gtttgaatgt cggaaacgcc
      961 gtgctgggac aaatgcgcgg ccgtttcgtg caatgttgcg gaatatggcg tgatgtgttc
     1021 ggcaaagcgt gtgtggtgca acgcttcgcg ccgttcccac agggtgctga cgacggatac
     1081 gccgacaccg cccatcagca cgcgcaagaa attcgacagg ctgccggcgg cggcaatctg
     1141 tccgcccttc atgtgcgaca gtgtgatggt ggtcagcggc aggaagaaca tggcaacgcc
     1201 tactccctgc caaaactgcg gccagatgac gttgccgata tccatgtcgg cataaaaatc
     1261 ggtgcgccaa tagaaagtga aggcaaaggt caggaagctg gcggttacga gcaggcgcat
     1321 atcgatttta ttgccgaacc tgccgattaa cggagacagg aagacgggca ggatgccgac
     1381 gggcgcggcg gcaagccccg cccacgtgga ggtatagccc aggttggtct gcaacactaa
     1441 cggcagcagg gtcagcgtcc ccatatacac cataaaaccc aatgatgtgg caatgatgcc
     1501 gacggtaaaa ttccgatctt taaacagcga taaatcgaca atcggatatt tttctcccaa
     1561 ttcccaaaca ataaaatacg acaagcacac cagtgcgact acgcccaagg taatgatttc
     1621 tccagaggcg aaccagtcga gttccttacc cctgtccaac atcatctgta acgcgccgat
     1681 accgactacc atcaatgtaa gcccgacata gtcggtcggc attttaacgg tttccgtttc
     1741 ccgacatttc aaatgtttcc atgtaatcca tgccgatatg ataccgatag ggatattaat
     1801 gaagaaaatc caaccccaat gccagtttcc ggaaatccag ccgccgagta tcggcccgag
     1861 aacaggggcg acaacgacgg tcattgccca caatgccagt gccagcgtcc gttttgcggg
     1921 cggataggat gccattaaca ggctttgcga caagggaatc agcggcccgg cgataaagcc
     1981 ctgcaagatg cggaaaacaa ccagcgactg aaggttgggg gcaataccgc acagccacga
     2041 tgtgatgacg aaaccgacag cggcggcggt aaacaatttg acctcgccga tacgttttgc
     2101 caaaaagccc gttaacggca cggaaacggc gtttgccaca gcaaaagaag taatgaccca
     2161 cgtcccctgc gtggttgccg caccgaggtt gccggcgatg acgggaacgg cgacattggc
     2221 gatagtcgta tctaaaactt ccataaatac ggcaagcccc aaagacagcg taacccacgc
     2281 caatgccgca cccttaagcg gtggataatc cattgtgtcc tccgtttcag acggcatcag
     2341 ggcgcggatt gcccgaggat ttcatcgacc gtccgatcga cttccgacca gtcggtactt
     2401 tccatttccg gcaatgccgc acccggcgtt tttgaaacag gcgcgcctgc ggcggaagta
     2461 tccactttaa ccgtcatcga caaaccgata cgcaacggat gcctgtccac atcttcgcgg
     2521 ttcagcacga tacggacggg gacgcgctgc accactttaa tccagttgcc cgttgcgttt
     2581 tgcgccggaa tcagcgaaaa cgcgctgccc gtacctgccg aaaaacctgc caccctgccg
     2641 cgataaacaa tttgtttgcc gtacaaatcg gacaccagct cggcaggctg tccgattttc
     2701 atatgccgca actgcgtctc tttaaaatta gcatccaccc acacatccga cagcggcacc
     2761 accgccatca gcggcgcgcc tgccgccacc tgctgcccga cctgcaccga acgcttcgcc
     2821 acctgaccgt ccgccggcgc gcggatttgc gtccgctgaa ggttcaacca cgcatctttc
     2881 aacctgccga ttgccgtctg aaccgccggc tgttcgcgca aagaaacctg accgcccaaa
     2941 gccgcacgtg ccgaagactc ttccgccaaa gccgccttaa ctgccgcctg agcctgagac
     3001 actgccgcac gggcatgtgc cagctcttcg gcggacactg cgcccgattc cgctaaagca
     3061 gaccggcggc gtaaatcatc ctgcgcacgt gccaaatccg ctcggcgcaa agcaacctgc
     3121 gcccccgcct gagaagtggc ggcattttgc tggcggtttt gccgcaccgc ctgaatcagc
     3181 tcgttttttg cccgctcgta agccagcaca tcattatcgt cgtccaacac cgccagcacg
     3241 tcgccttttt tcacggcatc cgtatcgtcg tgcaaaacct tccgcaccgt accgcccttt
     3301 tgcggcgtaa cctgaaccac gcgtccggca acataagcgt cttccgtttc ctcttcgtgc
     3361 tgccaccata aaaaaaacgc cgacccggcg gctgcggcgg caagcgcgaa cagcagcgtc
     3421 aatgccgtca ggcggcgttt gcgtttggct tgcgtgtttt gaagttttgt ttcgtccgcg
     3481 tgcgtatcca taagattggg ttccgaattt cctgatttca tataaacaat gttctttcaa
     3541 taggaagcag attctatatc cgcgcaataa cgcaatcaag tcagtatttt agatgcaata
     3601 ttcaaaaaac aacgcttcag gcggcaaaac tgaaaatata tttcgtttat ata
//
LOCUS       DAWHSK010000199         3607 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.199, whole genome shotgun sequence.
ACCESSION   DAWHSK010000199 DAWHSK010000000
VERSION     DAWHSK010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3607
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.199"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..1794
                     /gene="hscA"
                     /locus_tag="WIC03_001731"
     CDS             <1..1794
                     /gene="hscA"
                     /locus_tag="WIC03_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HEZ3884199.1"
                     /translation="IDLGTTNSLVATVRSGSAACLPDAEGRVTLPSVVRYLENGGIEV
                     GKTALSAQKTDPLNTVSSAKRLIGRTLADLHQNTHYLPYRFGDNQRVIELHTRQGVKT
                     PVEVSAEILKTLKSRAEETLGGDLVGVVITVPAYFDDAQRQATKDAARLAGLNVLRLL
                     NEPTAAAIAYGLDNASEGTFVVYDLGGGTFDVSVLQLTKGLFEVKATGGNSALGGDDF
                     DHRLFCRLLEQNGLSQLNEQDSQLLLSLVRAAKEQLTTQTEARIQATLSDGMAIDTSI
                     SRAEFHNLTQHLVMKTLEPVTQALKDAGVGKNEVKGVIMVGGSTRMLHVQQAVATFFG
                     QTPLNNLNPDEVVALGAAIQANVLAGNKTDGEWLLLDVTPLSLGLETYGGLAEKIIPR
                     NSTIPTARAQDFTTFKDGQTAMTIHVVQGERELVSDCRSLAKFTLRGIPPMAAGAARI
                     RVTFQIDADGLLSVSAQEQSTGVQAQIEVKPSYGLDDGAITRMLKDSMDNAAEDMAAR
                     ARAEAVVEAESLTDAVNAALELDSDLLDAKELQQIRQGIADLQGRLKDGKAEDIRSAV
                     AKLSRSTDNFAAKRMNRNIQRALTGQSVDNI"
     gene            1876..2532
                     /locus_tag="WIC03_001732"
     CDS             1876..2532
                     /locus_tag="WIC03_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEZ3884200.1"
                     /translation="MEKSGIVYLMKTVIKGVYKIGISDVSNFEGRMRHLENNGYANVA
                     GLERILAVKTDNYKEKENLLHEIFSKSRIGDTELFAVDENLVKRLFLSLRGEIVFPKN
                     ETAESEFEKSVHERRQEGNAGSGRKQLLDLVRRGHREYPYALPRLLAGAASYKPKKSK
                     IRLFKEAYLRKSGTRLTDEIADGIHIYTCFSRADLEKAYSEYLELFKSESDAEGRKPR
                     "
     gene            2568..3329
                     /locus_tag="WIC03_001733"
     CDS             2568..3329
                     /locus_tag="WIC03_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2314 domain-containing protein"
                     /protein_id="HEZ3884201.1"
                     /translation="MMGDSVIYYVEQADEPVNRAGERARKTFKYFWRELFWERRRIIP
                     ALDFAMVKAPFFQDGEDGEICEHMWIDDIYFDGLYIYGVLNNKPGELTNVEQGESICV
                     PVDDISDWMFVCNGIPYGGFTIQAMRGQMTEEERTEHDAAWGIDFGDPGQVLLVYEEK
                     EHPENLEEHPMCRNCIDDFRQQLSQNPDFLHEQDEDGYTPLHHEAIAGNALMVQAMLE
                     YGANPASKTSEGYTALDFARLTGWQNVADLLEPRH"
     gene            3384..>3607
                     /locus_tag="WIC03_001734"
     CDS             3384..>3607
                     /locus_tag="WIC03_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009425374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HEZ3884202.1"
                     /translation="MPKITVLPHTTLCPEGAVIDNAPEGKTVLDVLLDHDIEVDHACE
                     KSCACTTCHVIIRKGFDSLEEPTELEEDLL"
BASE COUNT          971 a         1007 c          923 g          706 t
ORIGIN      
        1 atcgatttgg gtacgaccaa cagcttggtt gccaccgtcc gcagcggcag tgccgcctgc
       61 ctgcccgatg ccgaagggcg cgttaccctg ccttccgtcg tccgctatct ggaaaacggc
      121 ggcattgaag tcggcaaaac cgccctgtcc gcccaaaaaa ccgacccgct gaacaccgtt
      181 agctccgcca aacgccttat cgggcggacg cttgccgatc tgcatcaaaa tacgcattac
      241 ctgccttacc gtttcggcga caatcaacgc gttatcgaac tgcatacgcg gcagggggtg
      301 aaaacgcctg tcgaagtgtc ggcggaaatc ctcaaaaccc ttaaatcgcg cgccgaagaa
      361 accttgggcg gcgatttggt cggcgtggtg attaccgtcc ctgcctattt cgatgatgcc
      421 caacgccaag ccaccaaaga tgccgcgcgt ctggcgggtt tgaacgtatt gcgcctgctc
      481 aacgaaccca ccgctgccgc aatcgcctac gggctggaca acgcctcgga aggcacgttt
      541 gtcgtgtacg acttaggggg cggcacattc gacgtatccg tattgcaact gaccaaagga
      601 ctgtttgaag tcaaagccac cggcggcaac agcgcgttgg gcggcgacga tttcgaccac
      661 cgcctgttct gccgcctgct cgaacaaaac ggactctccc aactcaacga acaagacagc
      721 caactcctgc tctcgctcgt ccgcgccgcc aaagaacaat taaccacgca aaccgaagcg
      781 cgcattcagg cgacgctttc agacggcatg gcaatcgaca caagcatcag tcgcgccgag
      841 ttccacaacc tgacgcagca tttggtgatg aaaacgctcg aaccggtcac acaggcgttg
      901 aaagatgccg gtgtcggtaa aaacgaagtc aaaggcgtga ttatggtcgg cggttcgacc
      961 cgtatgctgc acgtccaaca ggcagtcgcc accttcttcg gacaaacccc gctgaacaac
     1021 ctcaatcccg acgaagtcgt cgcgctcggc gccgccatac aggcaaacgt cctcgcaggc
     1081 aacaaaaccg acggcgaatg gctgctgctg gacgttacac ctttgtcgct cggtttggaa
     1141 acctacggcg gcttggcgga aaaaatcatc ccgcgcaatt ccaccatccc caccgcgcgc
     1201 gcgcaggact ttaccacctt caaagacggt caaaccgcga tgacgataca cgtcgtgcaa
     1261 ggagagcgcg agctcgtttc tgactgccgc agccttgcca aattcaccct gcgcggcatt
     1321 ccaccgatgg cggcgggtgc ggcgcgtatc cgcgtaacct tccaaatcga tgcggacggg
     1381 ctgctgtccg tttccgccca agaacaaagc accggcgtac aggcgcaaat cgaagtcaaa
     1441 ccttcctacg gcttggacga cggcgcgatt acccgaatgc ttaaagacag tatggacaac
     1501 gccgccgaag atatggcggc acgcgcccgc gccgaagccg tagtcgaagc cgaaagcctg
     1561 accgatgccg tcaacgccgc cctcgagttg gacagcgatt tactggatgc caaagaattg
     1621 caacagatcc ggcaaggcat cgccgatttg caaggccgtc tgaaagacgg aaaagccgaa
     1681 gacatccgct ccgccgtcgc caaactcagc cgcagcaccg acaacttcgc cgccaaacgc
     1741 atgaaccgca atatccaacg cgcgttgact ggtcaaagcg ttgataatat ttgacggcaa
     1801 atggtttcag acggcattgg ataaagaggt cgtctgaaaa aatatcatct cacagaaata
     1861 aaataaaaaa caacaatgga aaagtcaggc attgtctatc tgatgaaaac cgtcatcaag
     1921 ggcgtgtata aaatcggcat ttcggatgta agcaattttg aaggcagaat gcgccatttg
     1981 gaaaacaacg gttatgcgaa cgttgccggg ttggaacgca tcctcgccgt caaaaccgac
     2041 aattacaaag aaaaagaaaa cctgctccat gaaattttta gtaaaagcag gataggcgat
     2101 accgaattgt tcgccgtgga cgagaatctt gtaaaacgtt tgtttttatc gcttcgcggc
     2161 gaaatcgtgt tcccgaaaaa cgaaacggcg gaatcggaat ttgaaaaaag cgtccacgaa
     2221 cgcaggcagg aagggaatgc cggatccggt cgcaaacaac tgcttgattt ggtacggcgc
     2281 ggacaccggg aataccctta cgcgctgccc cggcttttgg cgggcgcggc atcctacaag
     2341 ccgaaaaaat cgaaaatccg cctttttaaa gaagcatatt tacgcaaaag cggtacgagg
     2401 ctgaccgacg aaattgcaga cggcatccat atttacacct gtttttcgcg ggcggatttg
     2461 gaaaaagcct attccgaata tttggaactt ttcaaatccg aatcggatgc cgaaggcaga
     2521 aaaccacggt aaggtgcaaa cagataccgt acacgttgag gagcagtatg atgggcgatt
     2581 ccgtcattta ttatgtagaa caggcagacg aaccggtaaa ccgtgccggc gaacgcgccc
     2641 gtaaaacatt caaatacttt tggcgcgagc ttttttggga acgccgccgc atcattcccg
     2701 ccttagattt tgccatggtc aaagcccctt ttttccaaga cggcgaagac ggcgaaattt
     2761 gcgaacatat gtggatcgac gatatttatt ttgacggtct ttacatttac ggtgtgctga
     2821 acaataaacc cggcgaactg actaatgtcg aacaaggcga aagcatttgc gttccggttg
     2881 acgacatcag cgactggatg ttcgtgtgca acggcattcc ctacggcggc tttaccatac
     2941 aggcaatgcg tggacagatg acggaagagg agcgtaccga acacgatgcc gcatggggaa
     3001 tcgatttcgg cgatcccggg caggtattgc tggtgtatga agaaaaagaa catcccgaaa
     3061 atctggaaga gcatccgatg tgccggaact gtattgacga ttttcggcaa cagttgtccc
     3121 aaaatccgga ttttctgcat gagcaggacg aagacggcta tacgccgctt catcatgaag
     3181 ccatcgcagg aaatgcactt atggttcaag ccatgcttga atacggcgca aatcctgcct
     3241 caaagacatc ggaaggctat accgccctcg attttgcccg cctgacgggc tggcaaaatg
     3301 ttgccgacct gctcgaaccg cgacattagg cagacagttt tccgaaaacg aacacaaaca
     3361 ctttttacag aaagacaata aaaatgccca aaatcaccgt acttccacac acgacattat
     3421 gccccgaggg tgcagtcatc gataacgcac ccgaaggtaa aaccgtcctt gacgtgctgc
     3481 tcgaccatga tatcgaagtc gatcacgcct gcgaaaaatc ctgcgcctgc acaacctgcc
     3541 acgtgattat ccgcaaaggt ttcgacagtc tggaagagcc gaccgaattg gaagaagacc
     3601 tgctcga
//
LOCUS       DAWHSK010000200         3596 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.200, whole genome shotgun sequence.
ACCESSION   DAWHSK010000200 DAWHSK010000000
VERSION     DAWHSK010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3596
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.200"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            100..1215
                     /gene="lst"
                     /locus_tag="WIC03_001735"
     CDS             100..1215
                     /gene="lst"
                     /locus_tag="WIC03_001735"
                     /EC_number="2.4.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyllactosaminide
                     alpha-2,3-sialyltransferase"
                     /protein_id="HEZ3884203.1"
                     /translation="MGLKKACLTVLCLIVFCFGIFYTFDRVNQGERNAVSLLKDKLFN
                     EEGEPVNLIFCYTILQMKVAERIMAQHPGERFYVVLMSENRNEKYDYYFNQIKDKAER
                     AYFFHLPYGLNKSFNFIPTMAELKVKAMLLPKVKRIYLASLEKVSIAAFLSTYPDAEI
                     KTFDDGTINLIQSSSYLGDEFSVNGTIKRNFARMMIGDWSIAKTRNASDEHYTIFKGL
                     KNIMDDGRRKMTYLPLFDASELKAGDETGGTVRILLGSPDKEMKEISEKAAKNFNIQY
                     VAPHPRQTYGLSGVTTLNSPYVIEDYILREIKKNPHTRYEIYTFFSGAALTMKDFPNV
                     HVYALKPASLPEDYWLKPVYALFTQSGIPILTFDDKD"
     gene            complement(1380..1838)
                     /locus_tag="WIC03_001736"
     CDS             complement(1380..1838)
                     /locus_tag="WIC03_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEZ3884204.1"
                     /translation="MNIQTRLAATALALFLSACGNGGAPAQPKGEISENRTAAFKSMM
                     PDFSRMGKMVKGEEPYDVEKFKQATASFAESSKKPFTLFESDPQGNGRALPAVWSDGA
                     KFEAEKTKFAAAVEKLNAAAQTGKLDEIKAAYGETGASCKSCHDSFRAPE"
     gene            2108..2929
                     /locus_tag="WIC03_001737"
     CDS             2108..2929
                     /locus_tag="WIC03_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEZ3884205.1"
                     /translation="MAVLITGASAGFGEAMCRAFVGAGYRVIGAARRADRLQALADEL
                     GALFYPLEMDVSRRESVENALNGIPDEFSDIDCLINNAGLALGLDTADKADFEDWETM
                     IQTNVLGLTFLTRKILPQMVERGGGYVMNLGSIAGNYAYAGSNVYGATKAFVRQFSLN
                     LRAELADKNIRVTNIEPGLCGNTEFSNVRFKGDDERAAGVYEGVEFIRPEDIAETALW
                     LYQRPAHMNVNTIEIMPVAQTFAGMKVIKKAVPEVREDFEKQSMSLFSRIRSWFK"
     gene            complement(2980..3426)
                     /gene="yciA"
                     /locus_tag="WIC03_001738"
     CDS             complement(2980..3426)
                     /gene="yciA"
                     /locus_tag="WIC03_001738"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HEZ3884206.1"
                     /translation="MQHEEGNRQRPQGELLLRTVAMPRDTNPNQDIFGGWIMSQMDLG
                     GGILAAEIARGRIVTVAVQEMNFIRPVKVGNVVCCYGHCVRVGNTSLQLKVDVWVKTL
                     MNDCVTEDRYLVTEAVFTYVAIDAEGNPRPIPKEGNPKLAGLLPTP"
BASE COUNT          843 a          786 c         1044 g          923 t
ORIGIN      
        1 tattcggtaa ctgtcggaat atctgctaaa attccgcatt tttccgtccc gggacactcg
       61 gggcgtatgt tcaatttgtc ggaatggagt tttagggata tgggcttgaa aaaggcttgt
      121 ttgaccgtgt tgtgcctgat tgttttttgt ttcgggatat tttatacatt tgaccgggta
      181 aatcaggggg aaaggaatgc ggtttccctg ctgaaggaca aactcttcaa tgaagagggg
      241 gaaccggtca atctgatttt ctgctatacc atattgcaga tgaaggtggc ggaaaggatt
      301 atggcgcagc atccggggga gcggttttat gtggtgctga tgtctgagaa caggaatgaa
      361 aaatacgatt attatttcaa tcagataaag gataaggcgg agcgggcgta ttttttccac
      421 ctgccctacg gtttgaacaa atcgtttaat ttcattccga cgatggcgga gctgaaggtg
      481 aaggcaatgc tgctgccgaa ggtcaagcgg atttatttgg caagtttgga aaaagtcagc
      541 attgccgcct ttttgagcac ttacccggat gcggaaatca aaacctttga cgacgggaca
      601 atcaatttaa ttcaaagcag cagctatttg ggtgatgagt tttctgtaaa cgggacgatc
      661 aagcggaatt ttgcccggat gatgatcgga gattggagca tcgccaaaac ccgtaatgct
      721 tccgacgagc attacacgat attcaagggt ttgaaaaaca ttatggacga cggccgccgc
      781 aagatgactt acctgccgct gttcgatgcg tccgaactga aggcggggga cgaaacgggc
      841 ggcacggtgc ggatactttt gggttcgccc gacaaggaga tgaaggaaat ttcggaaaag
      901 gcggcaaaaa acttcaacat acaatatgtc gcaccgcacc cccgccaaac ctacgggctt
      961 tccggcgtaa ccacattaaa ttcgccctat gtcatcgaag actatatttt gcgcgaaatt
     1021 aagaaaaacc cgcatacgag gtatgaaatt tatacctttt tcagcggtgc ggcgttgacg
     1081 atgaaggatt ttcccaatgt gcacgtttac gcattgaaac cggcttccct tccggaagat
     1141 tattggctca agccggtgta tgccctgttt acccaatccg gcatcccgat tttgacattt
     1201 gacgataaag attaatcgca tagcaaatca aaatagaaaa tggcggagtg cgtggggtaa
     1261 aaataaggat agcgtttttt catttggatt gacgataatt tccgattgct ttgcgcgtgc
     1321 tgaaatggca aagaaaatgc cgtctgaagg cttcagacgg cattgttttg ttttggatgt
     1381 tattcgggcg cgcggaaact gtcgtggcag gatttgcagc ttgcgccggt ttcgccgtag
     1441 gcggctttga tttcatccag tttgccggtt tgggcagcgg cgttgagttt ttcgacggcg
     1501 gcggcgaatt ttgttttttc ggcttcaaat tttgcaccat ccgaccaaac ggcgggcagg
     1561 gcgcggccgt tgccttgcgg atcggactca aaaagtgtga acggtttctt gctgctttcg
     1621 gcaaacgacg ctgtcgcctg tttgaatttt tcgacatcgt aaggctcttc gcctttgacc
     1681 attttgccca tacgcgagaa gtcgggcatc atagatttga acgcggcggt gcggttttcg
     1741 gaaatttcac ctttgggctg tgcgggcgcg ccgccgtttc cgcaggcgga aaggaatagg
     1801 gcgagggcgg ttgcggcaag gcgggtttga atattcatcg tgtttccttt tcggttgaaa
     1861 ccccgcccac ttggacatcc gtccttcggg gcggtagaat cagattttat ttgggaaggg
     1921 tgcaatccct tccgaatcag gacggcacat agggcgacgc tttatgtgcc gtcctgtgtg
     1981 ttgaaacata ttgaatgtgt cctgtcgtgg tggtatggtt gttgtcattt tcagccggcg
     2041 cgatgccgtc tgaagagcgt tatgatacct gaaacaggtt tgggaaacga gaaagaaagg
     2101 aaaaacaatg gctgttttaa ttaccggtgc ttcggcagga ttcggcgaag cgatgtgccg
     2161 cgcgtttgtc ggggcgggat accgcgttat cggtgcggcg cgccgtgcgg acaggcttca
     2221 ggccttggcg gatgaattgg gtgctttgtt ttaccctttg gaaatggacg tgtcgcgacg
     2281 cgagtcggtg gaaaacgcct taaacggcat ccccgatgaa ttttccgaca tcgactgcct
     2341 catcaacaat gccgggctgg ctttgggtct ggacacggcg gacaaggcgg attttgaaga
     2401 ttgggaaacg atgattcaaa ccaatgtttt gggtttgacg ttcctgacgc gcaaaatttt
     2461 gccgcaaatg gtggaacgcg gcggcggcta tgtgatgaat ttgggttcga ttgcaggcaa
     2521 ttatgcttat gccggcagca acgtttacgg ggcgaccaag gcgtttgtgc ggcagttcag
     2581 cctgaatttg cgcgcggagt tggcggataa gaacatccgc gttaccaata tcgagccggg
     2641 tttgtgcggc aatacggagt tttccaatgt gcgcttcaaa ggcgatgacg agagggcggc
     2701 gggcgtgtat gagggtgtgg aatttatccg ccccgaagat attgcggaaa ccgcattgtg
     2761 gctgtaccag cggccggcgc atatgaatgt gaacacgatt gaaattatgc ccgtggcgca
     2821 gacttttgca ggaatgaaag tgataaaaaa agccgtgccc gaagtgcggg aagactttga
     2881 aaaacagagt atgtcgctgt tttcccgcat caggtcctgg ttcaaatgat gcaaaatgcc
     2941 gtctgaagac agtttcagac ggcattttta cgggtatttt tacggagtag gcaataagcc
     3001 cgccaatttg gggttgcctt ctttcggaat cgggcgcgga ttgccttccg catcgatggc
     3061 aacataagtg aacacggctt cggttacgag gtagcggtct tcggtaacgc aatcgttcat
     3121 caaagttttc acccagacgt cgactttaag ctggagggaa gtgttgccca cgcggacgca
     3181 atgcccgtag cagcagacga cgttgccgac cttgaccggg cggatgaagt tcatttcctg
     3241 aacggcgacg gtaacgatgc gtccccgcgc gatttccgcc gccaatatgc cgccgcccaa
     3301 atccatctgc gacataatcc agccgccgaa aatgtcttgg ttgggattgg tatcgcgcgg
     3361 catagcgacg gtacgcagga gcagttcgcc ttgagggcgt tggcggttgc cttcttcgtg
     3421 ctgcataaag tttccttgtt ttattgaaat ataaatcgaa cctgcacccc tgcccgaaac
     3481 gggaagtcgg gaagtgtgta gtttaaccca tttgagacat acaagggcgg gcgcggaaca
     3541 gattcgcaag gcgtattgta gggtggggcc gtagcaactg aaccgtcatt cccacg
//
LOCUS       DAWHSK010000201         3584 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.201, whole genome shotgun sequence.
ACCESSION   DAWHSK010000201 DAWHSK010000000
VERSION     DAWHSK010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3584
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.201"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(82..1338)
                     /locus_tag="WIC03_001739"
     CDS             complement(82..1338)
                     /locus_tag="WIC03_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEZ3884207.1"
                     /translation="MNAAQLDHTAKVLAEMLTFKQPADAVLSAYFREHKKLGRQDRHE
                     IAETAFAALRHYQKISTALRRPHAQPRKAALAALVLGRSTNISQIKDLLDEEETEFLG
                     NLKARKTEFSDGLNTAAELPQWLVEQLQQHWNEEEILAFGRSINQAAPLDIRVNTLKG
                     KRDKVLPLLQAESADAEATPYSPWGIRLKNKIALNKHELFLDGTLEVQDEGSQLLALL
                     VGAKRGEIIVDFCAGAGGKTLAIGAQMANKGRIYAFDIAEKRLANLKPRMTRAGLTNI
                     HPERISSEHDTRIARLAGKADRVLVDAPCSGLGTLRRNPDLKYRQSAETVANLLEQQH
                     SILDAASKLVKPQGRLVYATCSVLPEENELQVERFLSEHPEFEPVNCAELLQSLKVDL
                     DTGKYLRLDSARHQTDGFFAAVLQRK"
     gene            complement(1686..2027)
                     /locus_tag="WIC03_001740"
     CDS             complement(1686..2027)
                     /locus_tag="WIC03_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884208.1"
                     /translation="MKNNVKNWTTKEVKQSLDKFNNILIKNTFLLQYLKKEFSASSAY
                     CLSMLPEEEDIYEILVNGNIIIDLEFNKHTNETVVINVTDVDEYLKTLTNESGRVFFT
                     LAKEIGKQKNI"
     gene            complement(2231..3088)
                     /locus_tag="WIC03_001741"
     CDS             complement(2231..3088)
                     /locus_tag="WIC03_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1853 family protein"
                     /protein_id="HEZ3884209.1"
                     /translation="MNYALDALWWKLTSQPVRDLASLLTAPPLWQSGCELGVRELLGE
                     RGFRYLLALDADPAPLTEHLTRHAPFGHRLGIYAEELLAFWFANAPHAELLAHNLTVS
                     GSDGNTQGAADFVVRLNGKPYHIELTCKYYGGGTGRLEDMRGLDPKDTLLGKAAKLTA
                     QLGLPHTSDGIRTLRQHGLPLDVKPVSIVRGIGFFPHGFHAFEPPLNPYGWRGIYIQD
                     WAEYGFKRQEVRYHLLDRMAYLAPARVAETETLNATEIRRIDQGLIAVLECRPDGFWH
                     EIERIMKAV"
     gene            complement(3149..>3584)
                     /locus_tag="WIC03_001742"
     CDS             complement(3149..>3584)
                     /locus_tag="WIC03_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEZ3884210.1"
                     /translation="SELLQTANRGEKVALVFGNETFGLSIEEVQACNRLMTINGNPDY
                     FSLNLAQAVQVVCYEIFSQTGSPMTHLQQEDHAATHEQIKGMVAHMESVMNDIGFFNR
                     RNGERLMRRMQSLFGRANTQTEDIDILRGFFNTVSHRIHKKD"
BASE COUNT          798 a          806 c          955 g         1025 t
ORIGIN      
        1 tttttgttaa tccactataa ttgatgaaat gccgtctgaa cgctttgaaa agggttcgga
       61 cggcattttg ttcaaaccgg tttatttgcg ttgcaatacg gcggcgaaga agccgtcggt
      121 ttggtgtcgg gcggaatcga ggcgcaggta ttttccggta tccaaatcaa ccttcaggct
      181 ttgcagcagt tcggcgcagt tgacgggttc aaattcggga tgttcggaca ggaaacgttc
      241 gacttgcagc tcgttttctt ccggcaacac gctgcaggtg gcgtacacca agcggccttg
      301 cggtttcacc agtttggatg cggcatcgag gatgctgtgt tgctgttcca aaaggttggc
      361 cacggtctcg gcggattggc ggtatttgag gtcgggattg cggcgcagcg tgcctaagcc
      421 ggagcagggc gcatccacta aaacgcggtc ggctttgcct gccagtcggg caatacgggt
      481 atcgtgttca ctgctgatgc gttcagggtg gatgttggtc agtccggcac gggtcatgcg
      541 cggtttgagg ttggcaaggc gtttttcggc gatgtcgaag gcgtagattc tgcctttgtt
      601 cgccatttgc gcgccgatgg cgagggtttt gccgcctgcg ccggcacaga aatcgacaat
      661 gatttcgccg cgttttgcgc ccaccaataa agcaagcagc tggctgcctt cgtcttggac
      721 ttccagtgtg ccgtccaaaa acagctcgtg tttgttaagc gcgattttgt ttttcaggcg
      781 gatgccccaa ggcgaataag gcgttgcctc cgcatcggca ctttcggctt gcaacaatgg
      841 cagcacttta tcgcgtttgc ctttcaaggt attgacgcgg atgtcgagcg gcgcggcttg
      901 gttgatgctg cgtccgaagg cgaggatttc ttcttcgttc caatgctgtt gcagttgttc
      961 gaccagccat tgcggcaatt ctgcggcggt attcagaccg tctgaaaact cggttttgcg
     1021 ggctttcaaa ttgccgagga actctgtttc ttcttcatca agcaggtctt tgatttggct
     1081 gatgttggtg cttctgccga gaaccagtgc ggcgagtgcg gctttgcgcg gctgcgcgtg
     1141 aggacgacgc agggcggtac tgattttttg atagtggcgc agcgcggcaa aggcagtttc
     1201 ggcgatttcg tggcggtctt ggcggccgag ttttttgtgt tcgcggaaat aggcggagag
     1261 gacggcatcg gcaggctgtt tgaaagtcag catttcagcc aaaactttgg cggtatggtc
     1321 gagttgtgcg gcgttcataa tgtaatgggt tctcttactg tgggttattc ataatcaatc
     1381 taacgtcctt aaaataacaa tagcatcttt atcattactt agtattctat ccgcaataag
     1441 aaagatgtgt taaaagtgcg caatcgggat ttgtctaaat ttctccgtta actacaaagg
     1501 cagcctgaaa aaagttctat tttcaggtcg cttgaacagc atccgatata atcacatcag
     1561 cgatttcatt ttgtaatcac tccctaaaac atgaggaaaa aaacaaaatt tattttcttc
     1621 gttcagtata tctatcctaa atatgccatc actccattga gtttttaatt tgtatttcgc
     1681 tcttgttaaa tgtttttctg tttgccgatt tcttttgcta atgtaaaaaa tactctacca
     1741 ctctcattgg ttaaagtttt caagtattca tcaacatcag taacattaat aacaactgtt
     1801 tcatttgtat gtttattaaa ttctaaatca ataataatat tcccattaac caatatttca
     1861 tatatatctt cttcttcagg gagcatagac aaacaataag cacttgaagc tgaaaactct
     1921 tttttcagat actgaagaag aaaagtattt ttaattaaaa tattattaaa tttatctaat
     1981 gattgcttga cttctttagt tgtccaattt ttaacattat tcttcattgt attctaccta
     2041 gggtcgtagt tattaaatta ccatacttat ttgtttggat cgtcataata ttagttggca
     2101 tgttgttgtt atatttatca tatccaatat ccttattaac tcggatgact ctttcataaa
     2161 ttgtatggga aatgagattt ccatcactaa gcctctcaat agatatacgc gttaatgttg
     2221 ggaaagggtt tcagacggcc ttcataatgc gttcgatttc gtgccaaaag ccgtccggcc
     2281 gacattccaa aacggcaatc aagccttggt cgatacggcg gatttcggtt gcgttcaatg
     2341 tttcggtttc ggcgacacgc gcaggcgcga ggtaggccat acggtcgagc agatggtagc
     2401 ggacttcttg gcgtttaaac ccgtattccg cccaatcttg aatatagatg ccgcgccaac
     2461 cgtatggatt aagcggtggc tcaaaagcat ggaaaccgtg tggaaaaaat ccgatgccgc
     2521 gcacgatgga aacgggtttt acgtcaagcg gcagaccgtg ttgccgcaag gttcggatgc
     2581 cgtctgaagt gtgcggcaga ccgagttggg cggtcagttt ggcggctttt cccaacagcg
     2641 tgtctttggg gtcgagtccg cgcatgtctt cgagcctgcc cgtgccgccg ccgtaatatt
     2701 tgcaggtcag ctcgatatgg tagggcttgc cgttaagcct taccacaaaa tccgccgcgc
     2761 cttgcgtatt gccgtccgaa ccggaaaccg tgaggttgtg cgcgagcagt tcggcgtgtg
     2821 gcgcgttggc aaaccaaaat gccagcagtt cttcggcgta aatgccgagg cggtgtccga
     2881 acggggcgtg tcgggtgagg tgttccgtca gcggcgcggg atcggcatcc aatgccaaaa
     2941 gatagcggaa accgcgttcc cccaatagtt cgcgcacgcc caattcgcaa ccgctttgcc
     3001 acaaaggcgg cgcagtcagc agcgaggcga ggtcgcggac gggttggctg gtgagcttcc
     3061 accataatgc gtctagggcg taattcatgc ttatccgaat atcagtcatg ccgtctgaaa
     3121 agctggaaat gttttcagac ggccttaatc agtctttttt atggatacga tggctgacgg
     3181 tattgaaaaa accgcgcagg atatcgatgt cttcggtttg cgtgttggcg cgtccgaaca
     3241 ggctctgcat acggcgcatc agacgctcgc cgttgcggcg gttgaaaaag ccgatgtcgt
     3301 tcatcacgct ttccatgtgg gcgaccatgc ctttgatttg ctcgtgggtc gcagcgtggt
     3361 cttcttgttg aagatgggtc atgggcgaac cggtttggct gaagatttcg tagcacacga
     3421 cctgcacggc ttgggcgagg ttgagcgaga aatagtcggg attgccgttg atggtcatca
     3481 gtcggttgca ggcttggact tcttcgatgc tcaagccgaa agtctcgttg ccgaaaacca
     3541 gtgccacttt ctcgcctcgg tttgcggtct gcagtaattc ggat
//
LOCUS       DAWHSK010000202         3546 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.202, whole genome shotgun sequence.
ACCESSION   DAWHSK010000202 DAWHSK010000000
VERSION     DAWHSK010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.202"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..239)
                     /locus_tag="WIC03_001743"
     CDS             complement(<1..239)
                     /locus_tag="WIC03_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ3884211.1"
                     /translation="MSTFFQQTAQAMIAKHIDCFPLLKLDQVIDWQPIEQYLNRQRTR
                     YLRDHRGRPAYPLLSMFKAVLLGQWHSLSDPELEH"
     gene            complement(463..2595)
                     /locus_tag="WIC03_001744"
     CDS             complement(463..2595)
                     /locus_tag="WIC03_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HEZ3884212.1"
                     /translation="MSIVSAPLPALSALIILAHYHGIAANPADIQHEFCTSAQSDLNE
                     TQWLLAAKSLGLKAKVVRQPIKRLAMATLPALVWCDDGNHFILAKTDGEGEHAQFLIQ
                     DLVTNKSAVLSFAEFSNRYSGKLILVASRASVLGSLAKFDFTWFIPAVIKYRRLFFEV
                     LVVSVVLQLFALITPLFFQVVMDKVLVHRGFSTLDVVSVALLVVSLFEIVLGGLRTYL
                     FAHTTSRIDVELGARLFRHLLSLPLSYFEHRRVGDTVARVRELEQIRNFLTGQALTSV
                     LDLAFSFIFLAVMWYYSSTLTWVVLASLPAYAFWSAFISPILRTRLNDKFARNADNQS
                     FLVESITAVGTVKAMAVEPQMTQRWDNQLAAYVASGFRVTKLAVVGQQGVQLIQKLVT
                     VATLWIGARLVIEGKLTVGQLIAFNMLSGQVAAPVIRLAQLWQDFQQVGISVARLGDI
                     LNAPTENASSHLALPDIRGEITFEHVDFRYKADGRLILQDLNLRIRAGEVLGIVGRSG
                     SGKSTLTKLVQRLYVPEQGRVLVDGNDLALAAPAWLRRQVGVVLQENVLLNRSIRDNI
                     ALTDTGMPLERIIEAAKLAGAHEFIMELPEGYGTVVGEQGAGLSGGQRQRIAIARALI
                     TNPRILIFDEATSALDYESERAIMQNMQAICANRTVLIIAHRLSTVKTAHRIIAMDKG
                     RIVEAGTQQELLAKPNGYYRYLYDLQNG"
     gene            complement(3396..>3546)
                     /locus_tag="WIC03_001745"
     CDS             complement(3396..>3546)
                     /locus_tag="WIC03_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ3884213.1"
                     /translation="FWNQAKRVLRKYNEIDRKSFPLFLKECKFRFNFGTPSRQLKILR
                     DWCGI"
BASE COUNT         1003 a          933 c          819 g          791 t
ORIGIN      
        1 ctgtgttcga gttcgggatc ggagaggctg tgccattgtc cgagcaggac ggctttgaac
       61 atggacaaca gcgggtaggc aggacggccg cggtggtctc tgaggtaacg ggttctttga
      121 cggttcaggt attgttcgat cggctgccaa tcaattacct ggtccaactt caatagtggg
      181 aagcagtcga tgtgcttggc aatcatggct tgcgcggttt gttgaaagaa ggtgctcatg
      241 gaaaatcccc taaatgtctt ggtgggaatt taggggattt tggggaattt tgcaaaggtc
      301 tctagatgag tgaaaaagaa gtgcaggctg cctaaaaaga cagaaaaagt ctttccggca
      361 gcctgcactt tggtttcatt tcagtcagta aacccagtaa acgacggtct gaaaacgcag
      421 aacgttacga aaaaagcagc ctacacgccc atcccccgcc ttctacccgt tctgtaaatc
      481 atacagatag cggtaatatc cgttcggctt cgccagcaat tcctgctgtg ttcccgcttc
      541 cacaatcctg cctttatcca tggcaatgat ccggtgtgcc gttttaacag tggacagacg
      601 gtgggcgata atcagcaccg tccggttggc gcaaatggcc tgcatgttct gcataatcgc
      661 tcgttcactt tcataatcca gcgcgctggt ggcttcatca aaaatcagaa tgcgcggatt
      721 ggtgattaac gcgcgggcaa tcgcaatacg ctgccgctgt ccgcccgaca agccggcccc
      781 ttgttcgccc accacggtgc cgtagccttc cggcagctcc ataataaact cgtgtgcgcc
      841 cgccagtttg gctgcttcga taatgcgttc cagcggcata cccgtatccg tcagcgcgat
      901 attgtcgcgt atgctgcggt tgagcagcac attctcctgc aagaccacgc cgacctgccg
      961 ccgcagccag gcaggagcgg ccaaagccaa atcgttgccg tccaccaaca cccgtccctg
     1021 ctccggtaca tacagacgct gcaccaattt ggtgagtgtg gatttgcccg accccgaacg
     1081 tcccacaatc cccagcactt cccccgcccg aatccgcagg ttcaaatcct gcaaaatcag
     1141 cctgccgtcc gccttatagc ggaaatcgac atgttcgaac gtaatctccc cccggatatc
     1201 gggcaaagcc aaatgcgaag acgcattctc ggtcggcgca ttcagaatat cccccaaacg
     1261 cgccaccgaa atccccacct gctggaaatc ctgccacaac tgcgccaaac ggataacagg
     1321 cgccgccacc tgtcccgaga gcatattaaa cgcaatcagc tgccccaccg tcagcttgcc
     1381 ctcaattacc agccgtgcgc caatccacaa cgtcgccacc gtcaccagct tctgaatcag
     1441 ctgcaccccc tgctggccga ccaccgccaa cttcgttacc cgaaatcccg aagccacata
     1501 agccgccaac tgattgtccc aacgctgcgt catctgcggc tccaccgcca tcgcctttac
     1561 cgtacccacc gcagtgatgc tttctactaa aaacgactgg ttgtctgcat tgcgcgcgaa
     1621 cttatcgttc agacgcgtcc gcagtatcgg actgataaat gccgaccaaa acgcataggc
     1681 aggcaacgaa gccaatacca cccaagtcag agtggagctg taataccaca tcaccgccag
     1741 aaagataaac gaaaacgcca aatccaacac cgaagtcagc gcctgaccgg tcaagaaatt
     1801 gcgaatctgc tccaattccc gcacccgagc caccgtatca cccactcgtc tgtgctcgaa
     1861 ataggataaa ggcagggaaa gcagatgccg gaacaaacgc gcgcccaatt ccacatcaat
     1921 acgtgaagtc gtatgtgcaa acagatacgt ccgcaaaccg cccaacacaa tctcaaacag
     1981 cgacaccacc aacaaagcca ccgacaccac atccaaagta gagaatcccc gatgtaccag
     2041 caccttgtcc atcaccactt ggaaaaacag aggcgtaatc agcgcaaaca gctgcaacac
     2101 caccgacacc accaatactt caaaaaacaa ccggcggtat ttgattaccg ccggaataaa
     2161 ccaggtaaag tcaaactttg ccaaactgcc caataccgaa gcgcgggaag caaccaatat
     2221 cagtttgccc gaatatctgt tagaaaattc ggcaaaagac aataccgcag acttattcgt
     2281 aaccaaatcc tgtatcaaaa attgggcatg ctcaccctca ccgtctgttt tggccaaaat
     2341 gaaatggttg ccgtcatcac accataccaa tgcgggtaaa gtcgccatag ccaaacgttt
     2401 aataggctgg cggactacct ttgccttcaa tcccaaagat ttggcggcta acagccattg
     2461 cgtttcattt aaatcgctct gtgcggaagt acaaaattca tgctgtatat cggcaggatt
     2521 ggcggcaatg ccgtggtaat gggcgaggat gatgagggcg gaaagggcgg ggagcggtgc
     2581 ggatacgata gacataaata aaatatagtt agattggatg tggataacgg ctggctggaa
     2641 aaggaatata ttaagtagaa agaaatatat aaataaaaca gcagaacgca ttgtaaggat
     2701 atatatggga attgtaaaga gaaagtatgg aaaagttctc gtttcaggaa ggtaaaacgg
     2761 cttaggaatc gagttagatg aggatgcctc gcacctctcg tgcctcctgc ataccgttaa
     2821 ggcacagggt taaggtgcag gctgctccga actctgttgc ggtcgggtaa tgttattttt
     2881 tgtgtttcag gcagcctgaa atatctgtat atttttgttt taaatagatt ttaaagattg
     2941 ataactgttc ttgacgattt ttcaagaaag gagtaaattt caagaaagga gtaaagtgac
     3001 ttattatcaa tgacaagcaa cgcgcgaagt gacaaggaaa actatctact taaattctaa
     3061 ggaggcttcg aatatcataa accaatcaga aacatagaga taaaaattat gtacaaatat
     3121 aatcctctta tacaatttat tgcacagttg attatgtctt atggagcaag cgtagggtgg
     3181 gcacttgctg ccccacgcgt ttcatatttc aaggcagcct aaaaccgtgt gggcataaat
     3241 gcctacccta catcccaaaa aacaagcgca gcctgcgtgt gtagggtgcg aactttcggc
     3301 aggtagacac gcggttttat agtttcaagc tgagttggga tgcttaagat aaagtacaaa
     3361 accttaaaaa ataagggggc tgtactagat tagccctaaa ttccacacca atcccgcaaa
     3421 atttttagct gtcgggacgg tgtgccgaag ttaaaccgaa atttgcattc tttcaagaac
     3481 agcgggaaag atttacgatc aatttcgttg tatttgcgca agacgcgttt tgcctgattc
     3541 caaaag
//
LOCUS       DAWHSK010000203         3542 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.203, whole genome shotgun sequence.
ACCESSION   DAWHSK010000203 DAWHSK010000000
VERSION     DAWHSK010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3542
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.203"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            186..1061
                     /locus_tag="WIC03_001746"
     CDS             186..1061
                     /locus_tag="WIC03_001746"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEZ3884214.1"
                     /translation="MLQGSLVALITPMNQDGSIHYEQLRDLIDWHIENGTDGIVAVGT
                     TGESATLSVEEHTAVIEAVVKHVAKRVPVIAGTGANNTVEAIALSQAAEKAGADFTLS
                     VVPYYNKPSQEGIYQHFKTIAEATSIPMIIYNVPGRTVVSMTNDTILRLAEIPNIVGV
                     KEASGNIGSNIELINRAPEGFVVLSGDDHTALPFMLCGGHGVITVAANAAPKLFADMC
                     RAALQGDIALARKLNDRLIPIYDTMFCEPSPAAPKWAVSALGRCEPHVRLPLVPLTEG
                     GQAKVRAALKASGQL"
     gene            1070..2266
                     /gene="bamC"
                     /locus_tag="WIC03_001747"
     CDS             1070..2266
                     /gene="bamC"
                     /locus_tag="WIC03_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HEZ3884215.1"
                     /translation="MPSEPFGRHNATNTLISITQDDTMTLIKPVIAALALIGLAACSG
                     SKTEQPKLDYQSRSHRLIKLEVPPDLNNPDQGSLYRLPAGSGAVRASDLEKRRTPAVQ
                     QPADAEVLKSVKGVRLERDGSQRWLVVDGKSPAEIWPLLKAFWQENGFDIKSEEPAIG
                     QMETEWAENRAKIPQDSLRRLFDTVGLGGIYSTGERDKFIVRIEQGKNGVSDIFFAHK
                     AMKEVYGGKDKDTTVWQPSPSDPNLEAAFLTRFMQYLGVDGQQAENASAKNPTLPAAN
                     EMARIEGKSLIVFGDYGRNWRRTALALDRIGLTVVGQNTERHAFLVQKAPNESNAVTE
                     QKPGLFKRLLGKGKAEKPAEQPELIVYAEPVANGSRIVLLNKDGSAYAGKDASALLGK
                     LHSELR"
     gene            complement(2323..3255)
                     /gene="pip"
                     /locus_tag="WIC03_001748"
     CDS             complement(2323..3255)
                     /gene="pip"
                     /locus_tag="WIC03_001748"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HEZ3884216.1"
                     /translation="MYEIKQPFHSGYLQVSEIHQIYWEESGNPDGVPVIFLHGGPGAG
                     ASPECRGFFNPDVFRIVIIDQRGCGRSRPYACAEDNTTWDLVADIEKVREMLGIRKWL
                     VFGGSWGSTLSLAYAQTHPERVKGLVLRGIFLCRPSETAWLNEAGGVSRIYPEQWQKF
                     VAPIAENRRNRLIEAYHGLLFHQDEEVCLSAAKAWADWESYLIRFEPEEVDEDAYASL
                     AIARLENHYFVNGGWLQGDRAILNNIGKIRHIPTIIVQGRYDLCTPMQSAWALSKAFP
                     EAELRVVQAGHRAFDPPLADALVQAVEDILPRLL"
BASE COUNT          935 a         1165 c          793 g          649 t
ORIGIN      
        1 cgacggcacc ggcataaacc ggcgaatata ataaaatgat ttcaaacagg aaaaatcaat
       61 ttccaccata cctttcggcg caaacgcccc acatctgcaa cccgccggat ttcacacctg
      121 cggtaaagta ggctacaatc agaagcatta ccgcccccta caccacaact gataaaaggt
      181 tctgtatgtt acaaggtagc ctggttgccc tgattacccc gatgaatcaa gacggcagca
      241 tccattacga acaactccgc gatttgatcg actggcacat tgaaaacggc acggacggca
      301 tcgttgccgt cggcacgaca ggcgaatccg ccaccctctc cgtcgaagaa cacaccgccg
      361 tcatcgaagc cgtcgtcaaa cacgttgcca aacgcgtccc cgtcatcgcc ggcacagggg
      421 caaacaacac cgtcgaagcc atcgcccttt cccaagccgc cgaaaaagcc ggtgcagact
      481 tcactctctc cgtcgtcccc tattacaaca agccctccca agaaggcatt taccaacatt
      541 tcaaaaccat cgccgaagcc acttcgattc cgatgattat ctacaacgtg cccggccgta
      601 ccgtcgtcag catgactaac gacactatcc tgcgccttgc cgaaatcccc aacatcgtcg
      661 gcgtgaaaga agccagcggc aacatcggca gcaacatcga actcatcaac cgcgcgcccg
      721 aaggcttcgt cgtcctttcc ggcgacgacc ataccgccct gccgttcatg ctgtgcggcg
      781 gacacggcgt gattaccgta gccgccaacg ccgcacccaa gctctttgcc gatatgtgcc
      841 gcgccgcgct gcaaggcgac attgccctcg cacgcaagct caatgaccgg ctgattccga
      901 tttacgacac catgttctgc gaacccagcc ccgccgcacc gaaatgggcg gtatccgccc
      961 tgggcagatg cgaaccgcac gtccgcctgc cgctcgttcc gctgacggaa ggcggacagg
     1021 caaaagtccg cgccgccctg aaagcctcag gacaactcta aatataacta tgccgtctga
     1081 accgttcgga cggcataacg caacaaacac tttaatatcc atcacacagg atgacacgat
     1141 gacccttatc aaacccgtca ttgccgcgct cgcactcatc gggcttgccg cctgctccgg
     1201 cagcaaaacc gaacagccca agctcgacta ccaaagccgg tcgcaccgcc tgatcaaact
     1261 tgaagtccca cccgatttga acaaccccga ccaaggcagc ctctaccgcc tgcctgccgg
     1321 ttcgggagcc gtccgcgcca gcgatttgga aaaacgccgc acacccgccg tccaacagcc
     1381 tgccgatgcc gaagtattga aaagcgtcaa aggtgtccgc ctcgagcgcg acggcagcca
     1441 acgttggctt gtcgttgacg gcaaatcccc tgccgaaatc tggccgcttc tgaaagcctt
     1501 ttggcaggaa aacggcttcg acatcaaatc cgaagaaccc gccatcggac aaatggaaac
     1561 cgagtgggcg gaaaaccgtg ccaaaatccc ccaagacagc ttgcgccgcc tattcgacac
     1621 agtcggtttg ggcggcatct actccaccgg cgagcgcgac aaattcatcg tccgtatcga
     1681 acagggcaaa aacggcgttt ccgacatctt cttcgcccac aaagccatga aagaagtgta
     1741 cggcggcaaa gacaaagaca cgaccgtatg gcagccctcc ccgtccgatc ccaacctcga
     1801 agccgctttc ctgacgcgct ttatgcaata tttgggcgtt gacggacagc aggcggaaaa
     1861 cgcatcggca aaaaacccga ccctgcccgc cgccaacgaa atggcgcgta tcgaaggcaa
     1921 aagcctgatt gtctttggcg actacggcag aaactggcgg cgcaccgcgc tcgccctcga
     1981 ccgcatcggg ctgaccgtcg tcggtcaaaa caccgaacgc cacgctttcc tggttcaaaa
     2041 agccccgaac gaaagcaatg cagttaccga acaaaaaccc ggcctgttca aacgcctgct
     2101 gggcaaaggc aaagcggaga aacctgccga acagccggaa ctgattgtct atgccgagcc
     2161 tgtcgccaac ggctcgcgca tcgtcctgct caacaaagac ggcagcgcat atgccggcaa
     2221 agacgcatcc gcattattgg gcaaactcca ttccgaactg cgttaaacca ttcaaaacaa
     2281 aagcagcttc caaacagaag ctgctttttt atgcggaact ttttacaaca aacggggcaa
     2341 aatatcctca accgcctgaa ccagcgcatc cgccaaaggc ggatcgaacg cacgatgccc
     2401 tgcctgaacc accctcaatt ccgcttccgg aaaggctttc gacagcgccc acgcactctg
     2461 catcggcgta cacaaatcat accgcccctg cacgataatg gtcgggatat gccgtatttt
     2521 gccgatattg ttcaaaatcg ccctatcgcc ctgcaaccag ccgccgttga caaaataatg
     2581 gttttccaaa cgcgcgattg ccagcgaggc ataagcatct tcatccactt cctccggctc
     2641 gaaacggatc agatagcttt cccaatccgc ccaagccttt gcggcagaca ggcacacttc
     2701 ttcatcttga tgaaacagca acccgtgata cgcctcaatc agccggttcc gtcgattttc
     2761 agcaatcggc gcgacaaatt tttgccattg ttccggataa atccggctca caccgcccgc
     2821 ctcgttcagc cacgccgttt cagacggcct gcacaaaaat atcccgcgca ataccaatcc
     2881 ctttacccgt tcaggatggg tttgggcata agccagcgac aaagtgctgc cccacgaacc
     2941 gccgaacacc agccatttcc tgatacccag catttcacgg actttttcaa tatccgccac
     3001 caaatcccaa gtcgtattgt cttccgcaca agcatacggg cgcgaacgtc cgcaaccgcg
     3061 ctggtcgatg atgacgatgc ggaacacatc gggattgaaa aaaccccgac attcaggcga
     3121 agcccccgcg cccggcccgc catgtaaaaa aataaccggc acaccgtcgg gattgcccga
     3181 ttcctcccaa taaatttgat gaatttcaga cacctgcaag tatccgctat gaaaaggctg
     3241 ttttatctca tacatcatct tctcccatat tgcttctgac aatccgaaca ttcccacacg
     3301 caacatcacg cacgagacac aagccgcccc gaaaggcaaa tgccgaaaaa tcgcgatata
     3361 gtgaattaac aaaaatcagg acaaggcggc gagccgcaga cagtacggat agtacggaac
     3421 cgattcactc gtgcttaagc accttagaga atcattctct ttgagctaag gcgaggcaac
     3481 gccgtaccgg tttttgttaa tccactataa cagcaaccct gtcgccgtca ttcccgcaaa
     3541 ag
//
LOCUS       DAWHSK010000204         3480 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.204, whole genome shotgun sequence.
ACCESSION   DAWHSK010000204 DAWHSK010000000
VERSION     DAWHSK010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3480
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.204"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            36..701
                     /locus_tag="WIC03_001749"
     CDS             36..701
                     /locus_tag="WIC03_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tfp pilus assembly protein FimT/FimU"
                     /protein_id="HEZ3884217.1"
                     /translation="MCTRKQQGFTLTELLIVMVIAAIMAMIALPNMSQWIASRRIASH
                     AERIANLLRFSRGEAVRLNLPVYICPVQVKKGGAPNNRCDFSKKGRGMLAFGDKNGNK
                     AYDGDAADVFLRSVVLNDTDDSRINYAFNHIAFGSSQPTADRVVWTFNQNGTFGYTKD
                     QHLTSQSSFFYSDGYIQIVLTDARAVSDADKKFRSAVVLINSSGRVEVCPRNDRRTVC
                     QYK"
     gene            731..1351
                     /gene="pilV"
                     /locus_tag="WIC03_001750"
     CDS             731..1351
                     /gene="pilV"
                     /locus_tag="WIC03_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HEZ3884218.1"
                     /translation="MKNNDCFRLKNPQSGMALIEVLVAMLVLTIGILALLSVQLRTVA
                     SVREAETQTIVSQITQNLMEGMLMNPTIDSDSNKKNYNLYMGNHTLPHTLSAVDGDFA
                     IDAMKTKGQLAEAQLKRFSYELKNALPDAAAIHYTVCKDSSGNAPTLSGSTFSQNCDG
                     SANGDTLIKVLWVNDSAGDSDISRTNLEVSGDNIVYTYQARVGGRE"
     gene            1348..2313
                     /locus_tag="WIC03_001751"
     CDS             1348..2313
                     /locus_tag="WIC03_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HEZ3884219.1"
                     /translation="MRRKMLNVPKGSYDGMKGFTIIEFLVAGMLSMIVLMAVGSSYFT
                     SRKLNDAANERLAIQQDLRNAATLIVRDARMAGSFGCFNMSEHTEKNVVSDVAQKNRL
                     FSLKGSSANKLIPNGTDNKLIPITESLDIGYQGFTQRLNALVFQYGIDDLDASAETVV
                     VSSCSKIAKPGKKISTLQEAKSALQITNDDKQNGNITRQRYVVNAYAVGRIAGEEGLF
                     RFQLDDKGKWGNPQLLVKKINKMDIRYIYVSDCPEDDDAGKEEKFKYTGTFDSSTNAV
                     TPAGVEVLLSSGTDTKIAASSDNHIYAYRIDATIRGGNVCANRTL"
     gene            2292..2891
                     /locus_tag="WIC03_001752"
     CDS             2292..2891
                     /locus_tag="WIC03_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HEZ3884220.1"
                     /translation="MRKQNTLTGIPTSDGQRGFALFIVLMVMIVVAFLVVTAAQSYNT
                     EQRISANESDRKLALSLAEAALREGELQVLDLEYDTDSKVTFSENCGEGLCTAVNVRT
                     NTNNDNEEAFDNIVVQGKPTVEAVKRFCPANSTDLCIDKKGMEYKKGTRSVSKPPRYI
                     IEYLGVKNGENVYRVTAKAWGKNANTVVVLQSYVSNNDE"
     gene            2896..3369
                     /locus_tag="WIC03_001753"
     CDS             2896..3369
                     /locus_tag="WIC03_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEZ3884221.1"
                     /translation="MEQKGFTLIEMMIVVAILGIISVIAIPSYQSYIEKGYQSQLYTE
                     MVGINNISKQFILKNPLDDNQTIKSKLEIFVSGYKMNPKIAEKYNVSVHFVNKEKPRA
                     YSLVGVPKTGTGYTLSVWMNSVGDGYKCRDKASAEAYSETLSADAGCEAFSNRKK"
BASE COUNT         1003 a          687 c          884 g          906 t
ORIGIN      
        1 aatttaatcc acgatacatt accagttaac gtcctatgtg tacacgaaaa caacaaggtt
       61 tcacgctaac agagctgctc atcgtgatgg tcattgcagc cattatggcg atgatagccc
      121 tccccaatat gagccaatgg attgcatccc gccgcattgc cagtcatgcg gagcggattg
      181 ccaacctttt gcgtttctcc aggggagaag ccgtccggct caatcttcct gtctatatct
      241 gtcctgttca agttaaaaaa ggcggtgcgc ccaacaatag atgtgacttc agcaagaagg
      301 ggcggggaat gttggctttc ggcgacaaaa acggcaataa ggcatatgac ggcgatgcgg
      361 cggatgtttt cctccgcagc gtggtgttga atgataccga cgacagccgg attaattatg
      421 ccttcaacca tatcgctttc ggttcgtctc agccgaccgc cgaccgtgta gtttggacat
      481 tcaatcaaaa cgggacgttc ggttatacga aagaccagca tcttacaagc caatccagct
      541 ttttttattc cgacggttat atccaaatcg tgctgacaga tgcgagagca gtttcagatg
      601 ccgataagaa attccgttcg gcggtggttt tgattaacag cagcggcagg gttgaggttt
      661 gtcctagaaa cgataggcgt accgtatgcc aatataaatg attgttttag tcggtttggg
      721 aaataaaaaa atgaagaata atgattgctt ccgcctgaaa aacccccagt ccggtatggc
      781 gctgatagaa gtcttggtcg ctatgctcgt tctgaccatc ggtattttgg cactattgtc
      841 tgttcagttg cggacagtcg cttccgtcag ggaggcagag acgcaaacca tcgtcagcca
      901 aatcacgcaa aacctgatgg aaggaatgtt gatgaatccg accattgatt cggacagcaa
      961 caagaaaaac tataatcttt acatgggaaa ccatacacta ccgcatacac tatcagctgt
     1021 ggatggcgat tttgcgattg atgccatgaa aactaagggg caattggcag aggcacaatt
     1081 gaagagattt agttatgagc tgaaaaatgc cttgccggat gcggcagcca tccattacac
     1141 cgtctgcaag gattcgtcgg gtaatgcgcc gacattgtcc ggcagtactt tttctcaaaa
     1201 ttgcgatggt agtgcaaatg gggatacttt gattaaagta ttgtgggtaa atgattcggc
     1261 aggggattcg gatatttccc gtacgaatct tgaggtgagc ggcgacaata tcgtatatac
     1321 ttatcaggca agggtcggag gtcgggaatg agacgtaaaa tgctaaacgt accaaaaggc
     1381 agttatgatg gtatgaaggg ttttaccatt attgaatttt tggttgcggg catgctcagt
     1441 atgattgtcc tgatggcggt cggatcgagt tacttcacgt cccggaaatt aaatgatgcg
     1501 gcaaacgagc gtcttgctat tcaacaggat ttgcggaatg cggcaacatt gattgtccgc
     1561 gatgcaagaa tggcggggag cttcggttgt ttcaatatgt ctgagcatac cgaaaagaat
     1621 gttgtttccg atgtggcgca aaaaaaccgt cttttttcct taaaagggag cagtgcaaat
     1681 aaacttatcc ccaacggtac agataataaa cttatcccca taacggaatc tttagatatc
     1741 ggatatcagg gttttaccca gcgccttaac gcattggttt tccaatacgg tatcgatgat
     1801 cttgatgcga gtgctgagac tgttgtagtc agcagctgtt ccaaaatagc aaaaccgggt
     1861 aagaaaatat ctaccttgca agaagcaaag agtgcattac agattactaa tgatgataaa
     1921 caaaatggaa atatcacccg tcaaaggtat gtggtcaatg cctatgcggt cggcaggatt
     1981 gccggtgagg aaggtttgtt ccgcttccaa ttggatgata agggcaagtg gggtaatcct
     2041 cagttgttgg ttaaaaagat taataaaatg gatatacggt atatttatgt ttccgattgt
     2101 cctgaagatg acgatgccgg caaagaggaa aaattcaaat atacgggtac attcgacagc
     2161 tccacaaatg ctgttacgcc cgccggggtg gaggttttat tgagtagcgg tactgatacc
     2221 aagattgccg cttcttcaga caatcatatt tatgcttacc gtatcgatgc gacaatacgc
     2281 gggggaaatg tatgcgcaaa cagaacactt tgacgggaat cccgacttct gacggacaga
     2341 gggggtttgc actgtttatc gtgctgatgg tgatgatcgt cgtggctttt ttggttgtaa
     2401 ctgccgcgca gtcttacaat accgagcagc ggatcagtgc caacgaatca gacaggaaat
     2461 tggctttgtc tttggccgag gcggctttgc gggaaggcga acttcaggtt ttggatttgg
     2521 aatatgatac ggacagtaag gttacattta gcgaaaactg tggagaaggt ctgtgtaccg
     2581 cagtgaatgt gcggacaaat acaaataatg ataatgaaga ggcttttgac aatatcgtgg
     2641 tgcaaggcaa gcccaccgtt gaggcggtga agcgtttttg ccctgcaaat tctaccgacc
     2701 tgtgcattga caagaaaggg atggaatata agaaaggcac gagaagcgtc agcaaaccac
     2761 cgcgctatat tatcgaatat ttgggcgtga agaacggaga aaatgtttat cgggttactg
     2821 ccaaggcttg gggtaagaat gccaataccg tggtcgtcct tcaatcttat gtaagcaata
     2881 atgatgagta ataaaatgga acaaaaaggg tttacattga ttgagatgat gatagtcgtc
     2941 gcgatactcg gcattatcag cgtcattgcc ataccttctt atcaaagtta tattgaaaaa
     3001 ggctatcagt cccagcttta tacggagatg gtcggtatca acaatatttc caaacagttt
     3061 attttgaaaa atcccctgga cgataatcag accatcaaga gcaaactgga aatatttgtc
     3121 tcaggctata agatgaatcc gaaaattgcc gaaaaatata atgtttcggt gcattttgtc
     3181 aataaggaaa aaccaagggc atacagcttg gtcggcgttc caaagacggg gacgggttat
     3241 actttgtcgg tatggatgaa cagcgtgggc gacggataca aatgccgtga taaggcttct
     3301 gccgaagcct attcggagac cttgtccgca gatgccggct gtgaagcctt ctctaatcgt
     3361 aaaaaataag gttgttttgc caataccgtc tgaaaatcaa tgttcagacg gtatttttat
     3421 gggtatagtg gattaacaaa aatcgggaca aggcgacgaa gccgaagaca gtacaaatag
//
LOCUS       DAWHSK010000205         3419 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.205, whole genome shotgun sequence.
ACCESSION   DAWHSK010000205 DAWHSK010000000
VERSION     DAWHSK010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3419
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.205"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            1..2003
                     /locus_tag="WIC03_001754"
                     /pseudo
     CDS             1..2003
                     /locus_tag="WIC03_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_180374912.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            2245..>3419
                     /locus_tag="WIC03_001755"
     CDS             2245..>3419
                     /locus_tag="WIC03_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ3884222.1"
                     /translation="MVIFYFCGKTFMPARNRWMLLLPLLASAAYAEETPREPDLRSRP
                     EFRLHEAEVKPIDREKVPGQVREKGKVLQIDGETLLKSPELLSRAMYSAVVSNNIAGI
                     RVILPIYLQQAQQDKMLALYAQGILAQADGRVKEAISHYRELIAAQPDAPAVRMRLAA
                     ALFENRQNEAAADQFDRLKAENLPPQLMEQVELYRKALRERDAWKVNGGFSVTREHNI
                     NQAPKRQQYGKWTFPKQVDGTAVNYRLGAEKKWSLKNGWYTTAGGDVSGRVYPGNKKF
                     NDMTAGVSGGIGFADRRKDAGLAVFHERRTYGNDAYSYTNGARLYFNRWQTPKWQTLS
                     SAEWGRLKNTRRARSDNTHLQISNSLVFYRNARQYWMGGLDFYRERNPADRGDNFNR"
BASE COUNT          890 a          817 c          979 g          733 t
ORIGIN      
        1 gtcgggttaa tctattaaca ttatctgttt tatcgctgtt ttgcacgcca tatgtttgag
       61 gttcggatgc gtacgatccc gtcaaagaag ccgagattaa aaacaaattt attttagaag
      121 cggcggaaga cagaaattcc cacgtttggc gcggcccgtg cagtgtgtct tttgattgct
      181 tcggtgcttt cagttatcag cccggttcgg atactcgttc tacaaatatt ggcgatgctg
      241 ccgatttttc attttcagac aagccggcaa acggcgtttc ccattatttt tccagcggta
      301 aaaccgatcc aaattcatct gaagccttgt atgacgaaat caatatcacg ggtaaaaact
      361 acaatagcgg catcctcgcc gtcgataata tgcccgttgt taagaaatat gttacagaga
      421 attacggagc tgatttaaag caggcggtta aggatcaatt aaaggattta tataaaacaa
      481 gacccgaaga ttggggaaaa aaataaaaaa atagttgagg aagcgataat aaaacaatat
      541 ggaataccta gaaataaaat taataaattt agaaaagtta ttgatccaat tatagaagaa
      601 gctgtactca ctcctcatag taatacaaca ccgaccaatc tcgacaacat cttcaataaa
      661 aaattacacg tcaaaatcga aaacaaatcc cacgtcgctg gacaggtgtt ggaactgacc
      721 aagatgacgc tgaaagattc cctttgggaa tcgcgccgcc attccgacat ccatacgctg
      781 gaaacttccg ataatgcccg catccgcctg aacacgaaag atgaaaaact gaccgtccat
      841 aaggattatg cgggcggcgc ggatttcctg ttcggctacg acgtgcggga gtcggacgaa
      901 cccgccctga cctttgaaga caaagtcagc ggacaatccg gcgtggtttt ggaacgccgg
      961 ccggaaaatc tgaaaacgct cgacgggcgc aaactgattg cggcaaaaac ggcggattcc
     1021 ggttcgtttg cgtttaaaca aaattaccgg cagggactgt acgaattatt gctcaagcaa
     1081 tgcgaaggcg gattttgctt gggcgtgcag cgtttggcta tccccgaggc ggaagcggtt
     1141 ttatatgccc aacaggctta tgcggcaaat actttgtttg ggctgcgtgc cgccgacagg
     1201 ggcgacgacg tgtatgccgc cgatccgtcc cgtcaaaata tacggggtgg cgcggctgcg
     1261 gacgggcggc gcaaaggcgt gcaaatcggc ggcgaggtgt ttgtacggca aaatgaaggc
     1321 agccggctgg caatcggcgt gatgggcggc agggtcggcc agcagcatca gttaacagca
     1381 aaggcggtgc ggcaggcagt gatttgcatg gttatggcgg gggtgtttat gctgcgtggc
     1441 atcagttgcg cgataaacaa acgggtgcgt atttggacgg ctggttgcaa taccaacgtt
     1501 tcaaacaccg catcaatgat gaaaaccgtg cggaacgcta caaaaccaaa ggttggacgg
     1561 cttctgtcga aggcggctac aacacgcttg tggcggaagg agttgtcgga aaaggcaata
     1621 atgtgcggtt ttacctgcaa ccgcaggcgc agtttaccta cttgggcgta aacggcggct
     1681 ttaccgacag cgaggggacg ggggtcggac tgctcggcag cggtcagtgg caaagccgcg
     1741 ccggcattcg ggcaaaaaac cgttttgctt tgcgtaatgg tgtcaatctt cagccttttg
     1801 ccgcttttaa tgttttgcac aggtcaaaat ctttcggcgt ggaaatggac ggcgaaaaac
     1861 agacgctggc aggcaggacg gcgctcgaag ggcggttcgg cattgaagcc ggttggaaag
     1921 gtcatatgtc cgcacgcatc ggatacggca aaaggacgga cggcgacaaa gaagccgcat
     1981 tgtcgctcaa atggctgttt tgatgcgtcg ggaaatgttt tgacgtacag gcggcacacc
     2041 ggcacggcac cgcacgccgc cccgcaaacc aatccgaacc ctgctgcccc gaagggcggg
     2101 gcataatgat gaaaccggcg gaaaaccgcc ggttttttgc cgccgtttga aacccgattc
     2161 tggcttcaga cggcattgtc gcggcatcgg gcggcagggt ttggaacagc ggcataaaaa
     2221 actgatacaa tccgccgatt gataatggtt attttttatt tttgtgggaa gacatttatg
     2281 cctgcacgaa acagatggat gctgctgctg cctttattgg caagtgcggc atatgccgaa
     2341 gaaacaccgc gcgaaccgga tttgagaagc cgtcccgagt tcaggcttca tgaagcggag
     2401 gtcaaaccaa tcgacaggga gaaggtaccg gggcaggtgc gggaaaaagg aaaagttttg
     2461 cagattgacg gagaaaccct gctgaaaagt cccgaattgt tgtcccgcgc gatgtattcc
     2521 gcagtggtct caaacaatat tgccggtatc cgcgttattt tgccgattta cctacaacag
     2581 gcgcagcagg ataagatgtt ggcactttat gcacaaggga ttttggcgca ggcagacggt
     2641 agggtgaagg aggcgatttc ccattaccgg gaattgattg ccgcccaacc cgacgcgccc
     2701 gccgtccgta tgcgtttggc ggcggcattg tttgaaaaca ggcagaacga ggcggcggca
     2761 gaccagttcg accgcctgaa ggcggaaaac ctgccgccgc agctgatgga gcaggtcgag
     2821 ctgtaccgca aggcattgcg cgaacgcgat gcgtggaagg taaatggcgg cttcagcgtc
     2881 acccgcgaac acaatatcaa ccaagccccg aaacggcagc agtacggcaa atggactttc
     2941 ccgaaacagg tggacggcac ggcggtcaat taccggctcg gcgcggagaa aaaatggtcg
     3001 ctgaaaaacg gctggtacac gacggcgggc ggcgacgtgt ccggcagggt ttatccgggg
     3061 aataagaaat tcaacgatat gacggcaggc gtttccggcg gcatcggttt tgccgaccgg
     3121 cgcaaagatg ccgggctggc agtgttccac gaacgccgca cctacggcaa cgacgcttat
     3181 tcttacacca acggcgcacg cctttatttc aaccgttggc aaaccccgaa atggcaaacg
     3241 ttgtcttcgg cggagtgggg gcgtttgaag aatacgcgcc gggcgcgttc cgacaatacc
     3301 catttgcaaa tttccaattc gctggtgttt taccggaatg cgcgccaata ttggatgggc
     3361 ggtttggatt tttaccgcga gcgcaacccc gccgaccggg gcgacaattt caaccgtta
//
LOCUS       DAWHSK010000206         3401 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.206, whole genome shotgun sequence.
ACCESSION   DAWHSK010000206 DAWHSK010000000
VERSION     DAWHSK010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3401
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.206"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..1467
                     /gene="aceF"
                     /locus_tag="WIC03_001756"
     CDS             <1..1467
                     /gene="aceF"
                     /locus_tag="WIC03_001756"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HEZ3884223.1"
                     /translation="TMDVPADAAGVVKEVKVKVGDKISEGGVILTVETGASAAEAAPA
                     VAAEAQPAPAAAPVAAGGATVQVAVPDIGGHTDVDVIAVEIKVGDTVAEDDTLITLET
                     DKATMDVPCTAAGVVKAVFLKVGDKVSEGSAIIEVETAGSVAAAPAQAAQAAAPAPTA
                     AAAPAAAPAPSAPAAAKIDEAAFAKAHAGPSARKLARELGVDLGQVKGTGLKGRIVGD
                     DIKAFVKSVMQGGAAKPAAAGASLGSGLDLLPWPKVDFSKFGNVEVKELSRIKKISGQ
                     NLSRNWVVIPHVTVHEEADMTELEEFRKQLNKEWEREGVKLSPLAFIIKASVSALKAF
                     PEFNASLDGDNLMLKNYFNIGFAADTPNGLVVPVIKDVDQKGLKQISQELTELSKKAR
                     EGKLKPQEMQGACFTISSLGGIGGTGFTPIVNAPEVAILGVCKSQIKPVWNGKEFAPR
                     LMCPLSLSFDHRVIDGAAGMRFTVFLAKLLKDFRRITL"
     gene            1561..1998
                     /locus_tag="WIC03_001757"
     CDS             1561..1998
                     /locus_tag="WIC03_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884224.1"
                     /translation="MGNFLYRGISCQQDEQNNGQLKPKGNKAEVAIRYDGKFKYDGKA
                     THGPSVKNAVYAHQIETGLYDGCYISTTTDKEIAKKFATSSGIENGYIYVLNRDLFGQ
                     YSIFEYEVEHPENPNEKEVTIRAEDCGCIPEEVIIAKELIEIN"
     gene            2016..>3401
                     /locus_tag="WIC03_001758"
     CDS             2016..>3401
                     /locus_tag="WIC03_001758"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEZ3884225.1"
                     /translation="MALVELKVPDIGGHENVDIIAVEVNVGDTIAVDDTLITLETDKA
                     TMDVPAEVAGVVKEVKVKVGDKISEGGLIVVVEAEGTAAAPKAEAPAAAPAQEAPKAA
                     APAPQAAQFGGSADAEYDVVVLGGGPGGYSAAFAAADEGLKVAIVERYKTLGGVCLNV
                     GCIPSKALLHNAAVIDEVRHLAANGIKYPEPELDIDMLRAYKDGVVSRLTGGLAGMAK
                     SRKVDVIQGDGQFLDPHHLEVSLTAGDAYEQAATTGEKKIVAFKNCIIAAGSRVTKLP
                     FIPEDPRIIDSSGALALKEVPGKLLIIGGGIIGLEMGTVYSTLGSRLDVVEMMDGLMQ
                     GADRDLVKVWQKQNEYRFDNIMVNTKTVAVEPKEDGVYVTFEGANAPKEPQRYDAVLV
                     AAGRAPNGKLISAEKAGVAVTDRGFIEVDKQMRTNVPHIYAIGDIVGQPMLAHKAVHE
                     GHVAAENCAGHK"
BASE COUNT          901 a          887 c          891 g          722 t
ORIGIN      
        1 acgatggatg tgcctgccga tgctgccggt gtcgtgaaag aagtaaaagt caaagtcggc
       61 gacaaaatct ccgaaggcgg cgtaattttg accgttgaaa ccggtgcctc cgccgccgaa
      121 gccgctccgg ctgttgccgc tgaagcacaa cctgcacctg ccgccgcacc cgttgccgca
      181 ggcggtgcaa ccgttcaagt agccgttccc gatatcggcg gtcataccga tgtggatgta
      241 atcgccgttg aaatcaaagt gggcgacacc gttgccgaag acgacacgct gattactttg
      301 gaaaccgaca aagcgacaat ggacgtacct tgtaccgctg ccggcgtcgt taaagccgta
      361 ttcttaaaag tcggcgacaa agtgtccgaa ggctctgcca ttatcgaagt ggaaaccgcc
      421 ggctctgtcg cagcagctcc tgctcaagcc gctcaagctg ccgcaccggc tccgactgct
      481 gccgccgcac ccgccgccgc gcctgcacct tctgcacctg ccgctgccaa aatcgacgag
      541 gccgctttcg ccaaagcaca cgccggccct tccgcacgca aactggcgcg cgaattgggc
      601 gtggatttgg gccaagtcaa aggtaccggc ttgaaaggcc gtatcgtggg cgacgacatc
      661 aaagcctttg tgaaatccgt aatgcagggt ggcgcggcaa aacctgccgc agccggcgca
      721 tctttgggca gcggtctgga cttgctgccg tggcctaaag tggacttctc caaattcggc
      781 aatgtcgaag ttaaagaatt gtcccgcatt aagaaaatct ccggtcaaaa cctgtctcgc
      841 aactgggtgg tgattcccca cgttaccgta cacgaagaag cagatatgac cgagctggaa
      901 gaattccgca aacaactgaa caaagaatgg gaacgcgaag gcgtgaaact gtctcctctg
      961 gcgttcatca tcaaagcctc cgtttccgcg ctgaaagcct tccccgaatt caacgcttct
     1021 ctggacggcg acaacctgat gctgaaaaac tacttcaaca tcggtttcgc agccgatacg
     1081 ccgaacggct tggttgttcc cgtcatcaaa gacgtggatc aaaaaggctt gaaacaaatc
     1141 agccaagagc tgacagaatt gtccaaaaaa gcccgcgaag gcaagctcaa accacaagaa
     1201 atgcaaggcg catgctttac catttccagc ttgggcggca tcggcggcac aggcttcacg
     1261 ccgattgtga acgctcccga agtcgccatc ttgggcgtgt gcaaatccca aatcaaaccg
     1321 gtttggaacg gcaaagagtt tgccccgcgc ctgatgtgcc cgttgagcct gtccttcgac
     1381 caccgcgtca tcgacggtgc ggccggtatg cgcttcaccg tattcttggc gaagctgttg
     1441 aaagacttcc gccgcattac cttataaaat aaaacatccc tctcaagcag tctgataatg
     1501 tttggattgc ttgagattga tgagtaatgg tgttaaattc aacctttaaa ttaataactt
     1561 atgggaaatt tcttatatag aggcattagt tgccaacaag atgagcaaaa taatggacag
     1621 ttaaaaccta aaggtaataa agctgaagtt gcaattcgtt atgatggtaa gtttaaatat
     1681 gatggtaaag ctacacatgg tccaagtgtg aagaatgcag tttacgccca tcaaattgaa
     1741 acaggtctat atgacggatg ttatatatct acgacaacag acaaggaaat tgccaagaaa
     1801 tttgcaacaa gttccggcat cgaaaatggc tatatatatg ttttaaatag ggatttgttt
     1861 ggtcaatatt ctatttttga atatgaggtt gaacatccag aaaacccaaa tgagaaggaa
     1921 gtaacaatca gagctgaaga ttgtggctgt attcctgaag aagtgattat tgctaaagag
     1981 ttgatagaaa ttaactaagt tgaaaggtca atataatggc tttagttgaa ttgaaagtgc
     2041 ccgacattgg cggacacgaa aatgtagata ttatcgcagt tgaagtgaac gtaggagaca
     2101 ccatcgccgt tgacgatacc ctgattaccc tggaaaccga caaagcgacg atggacgtgc
     2161 ctgccgaagt tgcgggcgtg gtcaaagaag tgaaagtcaa agtcggcgac aaaatctccg
     2221 aaggcggctt gattgtcgtc gttgaagctg aaggcacggc agccgctccc aaagctgaag
     2281 cacctgctgc cgccccggcg caagaagccc ctaaagctgc tgctcctgct ccgcaagccg
     2341 cgcaattcgg cggttctgcc gatgccgagt acgacgtggt cgtattgggt ggcggtcccg
     2401 gcggttactc cgctgcattt gccgctgccg atgaaggctt gaaagtcgcc atcgtcgaac
     2461 gttacaaaac tttgggcggc gtttgcctga acgtcggctg tatcccttcc aaagccttgt
     2521 tgcacaatgc cgccgttatc gacgaagtgc gccacttggc tgccaacggt atcaaatacc
     2581 ccgagccgga actcgacatc gatatgcttc gcgcctacaa agacggcgta gtttcccgcc
     2641 tcacgggcgg tttggcaggt atggcgaaaa gccgtaaagt ggacgttatc caaggcgacg
     2701 ggcaattctt agacccgcac cacttggaag tgtcgctgac tgccggcgac gcgtacgaac
     2761 aggcagccac taccggcgag aaaaaaatcg ttgccttcaa aaactgtatc attgcagcag
     2821 gcagccgcgt aaccaaactg cctttcattc ctgaagatcc gcgcatcatc gattccagcg
     2881 gcgcattggc tctgaaagaa gtaccgggca aactgctgat tatcggcggc ggcattatcg
     2941 gcctcgagat gggtacggtt tacagcacgc tgggttcgcg tttggatgtg gttgaaatga
     3001 tggacggcct gatgcaaggc gcagaccgcg acttggtgaa agtatggcaa aaacaaaacg
     3061 aataccgttt tgacaacatt atggtcaaca ccaaaaccgt tgcagtcgag ccgaaagaag
     3121 acggcgttta cgttaccttt gaaggtgcaa atgcaccgaa agagccgcaa cgctacgatg
     3181 ccgtattggt tgccgccggc cgcgcgccca acggcaaact catcagcgcg gaaaaagcag
     3241 gcgttgccgt aaccgatcgc ggcttcatcg aagtggacaa acaaatgcgt accaacgtgc
     3301 cgcacatcta cgccatcggc gacatcgtcg gtcaaccgat gttggcgcac aaagccgttc
     3361 acgaaggcca cgttgccgct gaaaactgcg ccggccacaa a
//
LOCUS       DAWHSK010000207         3376 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.207, whole genome shotgun sequence.
ACCESSION   DAWHSK010000207 DAWHSK010000000
VERSION     DAWHSK010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.207"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            61..690
                     /locus_tag="WIC03_001759"
     CDS             61..690
                     /locus_tag="WIC03_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HEZ3884226.1"
                     /translation="MSETENQALTFAKRLKADTTAVHDSVDNLVMSVQPFVSKENYIK
                     FLKLQSVFHKAVDHIYKDAELNKAIPELEYMARYDAVTQDLKDLGEEPYKFDKELPYE
                     TGNKAIGWLYCAEGSNLGAAFLFKHAQKLDYNGEHGARHLAPHPDGRGKHWRAFVEHL
                     NALGLSPEAEAEAIQGAREAFAFYKVVLRETFGLAADAEAPEGMMPHRH"
     gene            865..3227
                     /locus_tag="WIC03_001760"
                     /pseudo
     CDS             865..3227
                     /locus_tag="WIC03_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222141.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
BASE COUNT          920 a          847 c          837 g          772 t
ORIGIN      
        1 cgggcaagtg cggattttgc ccgcaccgca tccttatccc ttaccgacaa aggaaaactt
       61 atgagtgaaa ccgaaaatca agccctgacg tttgccaaac gtctgaaggc ggataccacg
      121 gcggttcacg acagcgtgga taacctcgtt atgtctgtcc aaccgtttgt cagcaaagaa
      181 aactacatca aatttttgaa actccaatcc gttttccaca aggccgtcga ccacatctat
      241 aaagatgccg aattaaacaa agccattccc gagctggaat acatggcgcg atacgatgcc
      301 gtaacgcaag acttgaaaga tttgggcgaa gagccttaca aattcgacaa agaattgcca
      361 tacgaaactg gcaacaaagc aatcggctgg ctttattgcg ccgaaggatc caatttgggc
      421 gcggcgtttt tgttcaaaca cgcccaaaaa ctcgattaca acggcgaaca cggcgcgcgc
      481 cacctcgctc cgcatcccga cgggcgcggc aaacactggc gcgccttcgt cgagcatctg
      541 aacgcattgg gcttaagccc cgaagccgaa gccgaagcca tccaaggcgc gcgcgaagcc
      601 tttgcattct acaaagtcgt gttgcgcgaa accttcggct tggcagccga tgccgaagca
      661 ccggaaggaa tgatgccgca caggcactaa aaaataatcg aaccaaataa acaaggtctc
      721 ggcatagctg tttgcagaga cctttaatta cacggcgcgg ctttgtttgc atggattact
      781 gtcttattaa atattaatga ttatcataaa atctattatt cgctaaccga tggatgaaca
      841 atccatacat cttgagttga taatatgaaa ccattacaaa tgctccctat tgccgcgctg
      901 gtcggcagta ttttcggcaa tccggttttg gcagcggatg aagctgcatc tgaaaccacg
      961 cccgttaagg cagagataaa agcagtgcgc gttaaaggcc agcgcaatgt gcctgcggct
     1021 gtggaacgcg tcaaccttaa ccgtatcaaa caagaaatga tacgcgacaa taaagacttg
     1081 gtgcgctatt ccaccgatgt cggcttgagc gacagcggcc gtcatcaaaa aggctttgcc
     1141 attcgcggcg tggaaggcaa ccgtgtcggc gttagtattg acggcgtaaa cctgcctgat
     1201 tccgaagaaa actcgctgta cgcccgttat ggcaacttca acagctcgcg tctgtctatc
     1261 gaccccgaac tcgtgcgcaa catcgacatc gtaaaagggg cggactcttt caataccggc
     1321 agcggcgcct tgggcggcgg tgtgaattac caaaccctgc aaggacgtga cttactgttg
     1381 cctgaacggc agttcggcgt gatgatgaaa aacggttaca gcacgcgtaa ccgtgaatgg
     1441 acaaataccc tcggtttcgg cgtgagcaac gaccgcgtgg atgccgcttt gctgtattcg
     1501 caacggcgcg gccatgaaac tgaaagcgcg ggcaagcgtg gttatccggt agagggtgct
     1561 ggtagcggag cgaatatccg tggttctgcg cgcggtattc ctgatccgtc ccaacacaaa
     1621 taccacagct tcttgggtaa gattgcttat caaatcaacg acaaccaccg catcggcgca
     1681 tcgctcaacg gtcagcaggg gcataattac acggttgaag agtcttacaa cctgcttgct
     1741 tcttattggc gtgaagctga cgatgtcaac agacggcgta acaccaacct cttttacgaa
     1801 tggacgccgg aatccgaccg gttgtctatg gtaaaagcgg atgtcgatta tcaaaaaacc
     1861 aaagtatctg cggtcaacta caaaggttcg ttcccgatag aggattcttc caccttgaca
     1921 cgtaactaca atcaaaagga cttggatgaa atctacaacc gcagtatgga tacccgcttc
     1981 aaacgcatta ccctgcgttt ggacagccat ccgttgcaac tcgggggggg cgacaccgcc
     2041 tgtcgtttaa aactttcgcc agccgccgtg attttgaaaa cctaaaccgc gacgattatt
     2101 acttcagcgg ccgtgttgtt cgaaccacca gcagtatcca gcatccggtg aaaaccacca
     2161 actacggttt ctcactgtct gaccaaattc aatggaacga cgtgttcagt agccgcgcag
     2221 gtatccgtta cgatcatacc aaaatgacgc ctcaggaatt gaatgccgag tgtcatgctt
     2281 gtgacaaaac accacctgca gccaacactt ataaaggctg gagcggtttt gtcggcttgg
     2341 cggcgcaact gaatcaggct tggcgtgtcg gttacgacat tacttccggc taccgtgtcc
     2401 ccaatgcgtc cgaagtgtat ttcacttaca accacggttc gggtaattgg ctgcccaatc
     2461 ccaacctgaa agccgagcgc agcaccaccc acaccctgtc tttgcagggg cgtggagaaa
     2521 aaggtacgct ggatgcgaat ttatatcaga gtaattacca taatttcctt tcagaagaac
     2581 agtttacacg acttatacgc gatgaaaagt gtgatgaaga ttatgctcta gcaggttatt
     2641 gtaaactcta ccgagatcag ctttattggc atatggaaaa tatcgacaag gccagaatcc
     2701 gcggtatcga gctgacaggc cgtctgaatg tggacaaagt agcgtctttc gtgccggaag
     2761 gctggaaact attcggctcg ctgggttacg cgaaaagcaa actgtcgggc gacaacagcc
     2821 tgctgtccac ccagccattg aaagtgattg ccggcatcga ttacgaaagc ccgagcgaaa
     2881 aatggggcgt gttctcccgt ctgacttatt tgggtgcgaa aaaggctaaa gacgcgcaat
     2941 atactgagta tgagggctac cgatttatca aaaaagtcaa agattatccg tggttgaata
     3001 agtcggctta tgtgttcgat atgtacggct tctacaaacc ggctaaaaac ctgactttgc
     3061 gtgcaggcgt gtacaatatg ttcaaccgca aatataccac ttgggactcc ctgcgcggcc
     3121 tgtatagtta cagtactacc aacgcagtag aaaaagatgg aagaggttta gatcgttacc
     3181 gcgcccccgg ccgcaactat gccgtatcgc tggaatggaa gttttaatct ggtattattg
     3241 gattaattgt ctcgttgggg attaaagccg tccgaattgt gttcaagaac tcattcggac
     3301 ggttttttac cgtaaatctg tgtgatgggg ttttatagtg gattaacaaa aatcaggaca
     3361 aggcgacgaa gccgca
//
LOCUS       DAWHSK010000208         3347 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.208, whole genome shotgun sequence.
ACCESSION   DAWHSK010000208 DAWHSK010000000
VERSION     DAWHSK010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3347
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.208"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            102..1344
                     /locus_tag="WIC03_001761"
                     /pseudo
     CDS             102..1344
                     /locus_tag="WIC03_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246189.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            complement(1454..1663)
                     /locus_tag="WIC03_001762"
     CDS             complement(1454..1663)
                     /locus_tag="WIC03_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884227.1"
                     /translation="MNYFGMVEFLRLMAMVRPPFVFFSGTRSELPAYLDLVAELRLTG
                     WERFAGSQTLTVSSTSTAIQATTTT"
     gene            complement(1660..1956)
                     /locus_tag="WIC03_001763"
     CDS             complement(1660..1956)
                     /locus_tag="WIC03_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrD"
                     /protein_id="HEZ3884228.1"
                     /translation="MDAKHRLKKYVYHLLVAIDQLFNALTGGAADETLSSRTYRGARL
                     AQKPKTRWKVLYTLINGVFFDRHHCRQAYISELKGRQHDARFNQSRAAGEKGTR"
     gene            complement(1935..2540)
                     /locus_tag="WIC03_001764"
     CDS             complement(1935..2540)
                     /locus_tag="WIC03_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884229.1"
                     /translation="MTIYFKNGFYDDTLGSIPEGAVAVRAEEYAALLAGQTQGGQIAA
                     DSDGRPVLTPPRPSEYHEWDGKKWEIGEAAAAARFAEQKTATAFRLAAKADELKNSLL
                     AGYPQVEIDSFYRQEKEALARQADNNAPTPMLAQIAAARGVELDVLIEKVVEKSARLA
                     VAAGAIIGKRQQLEDKLNTIETAPGLDALEKEIEEWTLNIG"
     gene            2620..2907
                     /locus_tag="WIC03_001765"
     CDS             2620..2907
                     /locus_tag="WIC03_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HEZ3884230.1"
                     /translation="MKIEFDSEKNQRNIEERNLPFESVGQIRWTTAVIVPDVRFDYPE
                     PRYVAAAYLGDTQRLHIVCFTPIKDGIRVISFRKANKREVKKYATSILNKR"
     gene            2879..3181
                     /locus_tag="WIC03_001766"
     CDS             2879..3181
                     /locus_tag="WIC03_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HEZ3884231.1"
                     /translation="MQRQSLTNADGEVRELAAEDFALARPIAEALPEDLAQVLFSHQK
                     QLEEKGVMQKRNTGKTPKQLVTIRLSADVVEKFRAGGKGWQTRINEVLRQYVAQLK"
BASE COUNT          715 a          898 c          888 g          846 t
ORIGIN      
        1 acacgcaatg taccctgacc ggcaaaactt ccccaaactt catcaaaaaa tcaactgtac
       61 ccgtcttttt cagacggcat tgataccgta agaaatttcc catgcagaaa tggcaaaccg
      121 agctttattc cacaccgctt tggcttttac agactctctt gatagtttcc gccgcttcgg
      181 cggtgattct gtttttgaca cggaaaacgc gtttcgggcg cgagtttgcc tatatcctgc
      241 gcctttgtct gacaccgaaa agcgcggtca aagtcttgct gctgattacg gagatgatta
      301 cgctggtgtt aaccgaaatg cggctgaatg tgttgagtac ctttatgtcc aaagggcttt
      361 acgactcgat gcaggatttg aaatgcttcg gcattttgga tgtttgccgc aatgaacgcg
      421 ggcgtggtgc tgatacgggc gtttaacaac gtcgtcaacg acttcctcaa tcaaggcttg
      481 gcgattaaat ggtcggagcg gctcaacgaa gtgctgacag cacgctggct tgccgacaaa
      541 aactactacc gcctgcaaat gcgccgccac gcgccggaca acatcgacca acgtatccaa
      601 caggacgcac aggatttcat cgcctcgacc atcgaatttg tgcgcggcat ggtcaattcg
      661 gtcgttacct cgctggagtt tgccgttgtt ttgtggggct tggcgggcat cctgaccgtg
      721 tttggctttg acattcctcg cggcattgtt tggttcgtct ttatctttgt aattttggcg
      781 acctttatcg ccatgtggat aggcaaccca ttgattcgtt acaactatga aaacgaaaag
      841 ctcaacggcg actaccgtta ttccctcatc cgcgtgcgcg accacgccga aagcgtggcg
      901 ttttacagcg gcgaaaaaca cgaacacgac cggcttgccg accgcttcaa agccattatc
      961 cgcaaccgct ggcgcattgc gcggcaaagc gtctgcctga gtggctttaa cgatatgttc
     1021 accaacggca tcaagctttt tcccattatt ttgcaagccc cacgcctgtt tgccggccaa
     1081 atcaaaatcg gcgacatcca gcagaccgtc caagccttcg cacgactgca aaacgcgctg
     1141 tctttcttcc ggatgttcta taacaaattc actgcctacc gcgcccgatt ggaacgtttg
     1201 tacggctttt tgctgagtac ggaagaaaaa cacagcacgc agcaacccga gattagcgaa
     1261 gtttcagacg gcattgcact ggaatgtaag gcggcggcag cttttgcacg aattggcggc
     1321 agcttttgca ccgccgaagc gtaggaaatt gtattagctt ttgcacaaat ttatccccgt
     1381 ttaatggtct gttaaacggg gattatctta tgcctttaaa agtagtacgc tacttgcggt
     1441 cagaacttat aaatcaggtg gtcgtcgtag cttgaattgc tgttgatgtg ctgctcacag
     1501 tcaaggtttg gctgcctgca aaacgctccc atccggtcag gcgcagttct gccacgaggt
     1561 caagataagc aggcagttcg ctgcgggtgc cggagaagaa gacaaatggc ggccgcacca
     1621 ttgccatcag gcgcagaaac tctaccatgc cgaagtagtt catcgcgtcc ccttttcccc
     1681 agcggcgcgg ctttggttga accgcgcgtc gtgctgcctg cctttcagtt cgctgatata
     1741 cgcctgtcgg cagtggtggc ggtcgaaaaa tacgccgttg atcagggtgt ataaaacctt
     1801 ccagcgggtt ttgggctttt gggcgagccg cgcgccgcga taggtgcggc ttgagagggt
     1861 ttcgtccgcc gcgccgccgg ttagggcgtt gaacagttgg tcgatggcta ccaacaggtg
     1921 gtaaacgtat tttttcagcc gatgtttagc gtccattctt cgatttcctt ttccagcgcg
     1981 tccaatcctg gcgcggtttc gatggtgttc aatttgtctt cgagctgctg ccgctttccg
     2041 ataatcgcgc cggcggcaac ggccaggcgg gcggattttt cgacaacttt ttcaatcaaa
     2101 acgtccaatt ccacgcccct tgcggcggcg atttgcgcca gcatcggggt cggggcgttg
     2161 ttgtccgcct gccgcgcgag ggcttctttt tcctgcctgt aaaagctgtc gatttccact
     2221 tggggatagc ccgccaagag gctgtttttg agttcgtccg cctttgccgc gaggcggaat
     2281 gccgtggcgg ttttttgttc ggcgaaacgg gcggcggcag cggcttcgcc gatttcccat
     2341 ttcttgccgt cccattcgtg gtattcggac gggcgcggcg gggttaaaac ggggcggccg
     2401 tcggaatctg cggcaatctg cccgccctgc gtctgtcctg ccaaaagggc ggcgtattct
     2461 tcggcgcgga cggcaaccgc gccttcgggg atgctgccca atgtgtcgtc gtaaaagccg
     2521 tttttgaaat agatggtcat ttcggggttt ccttttttgt atttacattt tggttttttg
     2581 ccgcttataa tgtaacgaca aaatacgctg caagctgcaa tgaagattga atttgactcc
     2641 gaaaaaaatc aacgcaatat cgaagagcgg aatttgccct ttgaatcagt cgggcaaata
     2701 cgctggacaa cggccgttat tgtgcccgat gtccgctttg attaccccga accgcgttat
     2761 gttgccgctg cttatctagg cgatacgcaa cgcctgcaca ttgtctgttt tacgccgata
     2821 aaagacggca tccgtgtcat cagttttcgc aaagcgaata aaagagaggt taaaaaatat
     2881 gcaacgtcaa tccttaacaa acgctgacgg cgaagtgcgg gaacttgccg ccgaagattt
     2941 tgcgcttgcc cgccccattg ccgaagccct gcccgaagac ttggcacagg tgcttttttc
     3001 ccatcaaaaa caattggaag aaaagggcgt aatgcaaaaa cgaaacacag gcaaaacccc
     3061 gaaacaactc gttaccatac gcctgtccgc cgatgtggtc gaaaaattcc gcgccggcgg
     3121 caagggctgg cagacgcgca tcaacgaggt tttgcgccaa tatgtcgccc aattgaaata
     3181 acgggggcgg ccgctgctta tcgggcgcgc ccgactatcc tttcccgcgc ggtttcggcc
     3241 gtgcggtttt ttgttgccgg tgccgtccga agggggcagc ggctttttgc gcggtcattc
     3301 ccgcctttcg ttattcccgc cactttccgt cattcccgcg aaggcgg
//
LOCUS       DAWHSK010000209         3332 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.209, whole genome shotgun sequence.
ACCESSION   DAWHSK010000209 DAWHSK010000000
VERSION     DAWHSK010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.209"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..785)
                     /locus_tag="WIC03_001767"
     CDS             complement(<1..785)
                     /locus_tag="WIC03_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-P-II uridylyltransferase"
                     /protein_id="HEZ3884232.1"
                     /translation="MPANLSSALETFKRQRDAAEAHYLKANRVSVFFREYTAAVETLL
                     AALWVEHFQNSALCLMAVGGFGRGELYPCSDVDLAVVSPAPLSDGIQEQIARFVQTLW
                     DCKLMPSVKSGSVDELCESVRNDITGDTAFLEARFLFGNRQTVDKLAEKMNAQRNVAA
                     FVEAKLVEMEHRHAKSQGSGAVLEPNIKSCPGGLRDIHTLLWIAKAQGLATDLPDLLK
                     QRILTRAEAGMLSHGYRRLAHIRIHLHLNANRAEDRLLFDLQP"
     gene            complement(833..1153)
                     /locus_tag="WIC03_001768"
     CDS             complement(833..1153)
                     /locus_tag="WIC03_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ3884233.1"
                     /translation="MMGNKLTPPAKLPDEQDLRAVLAYNMRLFRVNKGWSQEELARQC
                     GLDRTYVSAVERKRWNIALSNIEKMAAALGVAAYQLLLPPQERLKLMTNSADTRQMPS
                     ESGI"
     gene            complement(1478..1951)
                     /gene="bfr"
                     /locus_tag="WIC03_001769"
     CDS             complement(1478..1951)
                     /gene="bfr"
                     /locus_tag="WIC03_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEZ3884234.1"
                     /translation="MKGDRLVIRELNKNLGLLLVTINQYFLHARILKNWGFEELGEHF
                     FKQSIVEMKAADDLIERILFLEGLPNLQELGKLLIGESTEEIIACDLTKEQEKHEALL
                     AAIATAEAQQDYVSRDLLEKQKDTNEEHIDWLETQQELIGKIGLPNYLQTAAQED"
     gene            complement(1979..2443)
                     /gene="bfr"
                     /locus_tag="WIC03_001770"
     CDS             complement(1979..2443)
                     /gene="bfr"
                     /locus_tag="WIC03_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEZ3884235.1"
                     /translation="MQGNQAVLDYMNELLSGELAARDQYFIHSRLYSEWGYTKLFERL
                     NHEMEEETTHAEDFIRRILMLGGTPKMARAELNIGTDVVSCLKADLQTEYEVRDALKK
                     GIKLCEEAQDYVTRDLMVAQLKDTEEDHAHWLEQQLRLIELIGEGNYYQSQL"
     gene            complement(2906..>3332)
                     /locus_tag="WIC03_001771"
     CDS             complement(2906..>3332)
                     /locus_tag="WIC03_001771"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239803.1"
                     /note="catalyzes the radical-mediated insertion of two
                     sulfur atoms into an acyl carrier protein (ACP) bound to
                     an octanoyl group to produce a lipoyl group; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEZ3884236.1"
                     /translation="DVMNHNLETHPSLYRKARPGANYQHSLDLLKRYKEMMPHIPTKS
                     GIMVGLGETDEDVREIMRDMRAHNIEMITIGQYLQPSDGHLPVLRYVTPEQFKIFEKE
                     AYELGFSNAAIGAMVRSSYHADEQAAEALRESHGGCGHH"
BASE COUNT          772 a          799 c          837 g          924 t
ORIGIN      
        1 tgcggctgca aatcgaacag caggcggtct tcggcgcgat tggcgtttaa atgcagatgg
       61 atgcggatgt gggcgaggcg gcggtagccg tgcgaaagca taccggcttc ggcacgcgtc
      121 aaaatccgct gtttgagcag gtcgggcagg tcggtcgcca agccttgcgc cttcgctatc
      181 caaagcaggg tgtggatgtc gcgcagaccg cccggacagc ttttgatatt cggctccaat
      241 actgcccccg aaccttgcga tttggcgtgg cggtgttcca tctccaccag ttttgcctcg
      301 acaaacgccg ccacattgcg ctgcgcgttc attttttccg ccagtttatc caccgtttgg
      361 cggttgccaa acaaaaacct agcctctaaa aacgctgtgt cccccgtaat atcattgcgc
      421 acgctttcac atagttcatc aacgctgccg ctttttacag acggcatcag tttgcagtcc
      481 cacagggttt gaacaaaccg ggcaatctgt tcctgaatgc cgtctgaaag cggggcaggg
      541 gagacaaccg ccaaatccac atccgaacag ggatacagtt cgccgcgccc gaagccgccc
      601 accgccatca ggcataacgc gctgttttga aaatgttcta cccacaatgc cgccagtaag
      661 gtttcgactg ccgccgtata ttccctgaaa aataccgaca cgcggttggc tttcaaataa
      721 tgcgcttcgg cggcatcgcg ctgccgtttg aaggtttcca gtgctgaaga caggtttgca
      781 ggcattttta ttctttcgat tggcgggaaa agggaggtgg atggttcggc ggtcaaatac
      841 cgctttcaga cggcatttgt cgggtatcgg cggaattggt catcagcttc aaccgttcct
      901 gcggcggcag cagcaactga tacgccgcca cgcccaaagc cgccgccatt ttttcgatat
      961 tcgacagggc gatgttccag cgtttgcgct cgactgccga cacataagtc ctgtccaaac
     1021 cgcattgccg cgccaattct tcttgcgacc aacccttgtt cacgcggaaa agccgcatat
     1081 tgtatgccaa taccgcccgc aaatcctgtt cgtcaggcag tttggcaggc ggggtcaatt
     1141 tgttgcccat catttgcttc cagataaatg gttaaagtta agcatttgct gttacggatt
     1201 ttacttcaca taaaagccaa aaaaaagggg tggcggcagc cacacccaaa cacacacaca
     1261 tcaagaggaa agaggtaaaa tcaagacaga ttgggaaaca gcaaacaagc aatctagatt
     1321 tcatatcggt attatcaaac aaaaaccgtt atcgagtatt gattcaaatc aatttagatt
     1381 tgattcgcaa accggcaaag aaaaacggca tacccgttta tacggatatg ccgtctgatg
     1441 atgcagggga ctgctatatt tggcagtggt ttgtgtttta gtcctcttgc gccgctgttt
     1501 gcaggtagtt cggcaaacca attttgccga tcagttcctg ctgggtttca agccagtcga
     1561 tgtgttcttc gttggtgtct ttttgttttt ccaacaaatc acggctgacg taatcctgtt
     1621 gcgcttctgc tgtggcgatg gcggcaagca gggcttcgtg tttttcctgt tctttggtca
     1681 aatcgcaggc gatgatttct tcggtggact cgccaatcag aagcttgccc agttcttgca
     1741 ggttcggcag accttcgagg aacagaatgc gctcgatcaa atcgtcggca gctttcattt
     1801 caacgatgga ctgtttgaag aaatgttcgc ccagttcttc aaagccccag tttttcaaaa
     1861 tacgggcgtg aaggaaatat tggttgatgg ttaccagcag caagcctaag tttttgttta
     1921 gctcgcggat aaccagacgg tcgcctttca tacggactcc ttttattccg aatacggatt
     1981 acagttggct ttggtagtag ttgccttcgc caattaactc gatcaggcga agctgctgtt
     2041 ccagccagtg ggcgtggtct tcttcggtat ctttcagttg ggcaaccatc aggtcgcgcg
     2101 taacatagtc ttgagcctct tcgcacagtt tgatgccttt tttcaacgca tcacgtactt
     2161 catattcggt ttgcaggtcg gctttgaggc aggaaaccac gtccgtgccg atattcagtt
     2221 cggcgcgtgc cattttcggc gtgccgccca gcatcaggat gcggcggatg aagtcttcgg
     2281 cgtgtgtggt ttcttcttcc atctcgtggt tgagacgttc aaaaagtttg gtgtagcccc
     2341 attcggagta gaggcgggag tggataaagt attggtcgcg tgccgccagc tcgccagaca
     2401 gcaattcgtt catgtaatct aaaactgctt gattgccttg cataatattt ctcttttctt
     2461 aatttgggtt tcgtgtagtt aaacagtgga tgtagcactg ttcggaatgc gtgcattgta
     2521 tgcaaaagct gtcggcatga caaattttct atttaaaata caaacaatta tcaaaagaaa
     2581 tagggcgtat tccccgatgc cttccaaatc agtatgctgt tttttatcgg ttttttgtgc
     2641 tgttaaaact aataatccat attatccata aagaacattt acacttgtta accatagcta
     2701 aaagcaaata cggtacggtt ttttatcaaa atttataatg aatcgttctc attaaccgac
     2761 agattctttt gagattatcg gattttggga ataaattatg ccgtctgagg ggctttcaga
     2821 cggcatagac aattaaagct gattaaacaa cgcggcagac agcagcgtag gaaattattt
     2881 tttacttcgg aacggtattt tgggtttaat gatgcccgca accgccgtgg ctttcgcgta
     2941 aggcttcggc agcctgttcg tcggcgtggt agctggaacg taccatcgcg ccgatggcgg
     3001 cattgctgaa gcccagttcg tatgcttctt tttcaaagat tttgaactgc tcgggcgtaa
     3061 cgtagcgcag gacgggcagg tgtccgtctg aaggctggag gtactgtccg atggtaatca
     3121 tttcgatatt gtgcgcccgc atatcgcgca taatttcacg cacgtcttcg tctgtttcgc
     3181 ccaagccgac catgatgccg gatttggtcg ggatgtgcgg catcatttct ttataacgtt
     3241 ttaataagtc taaagaatgt tgataattgg caccgggacg ggcttttctg tacaggctcg
     3301 gatgggtttc taggttgtgg ttcatcacgt cg
//
LOCUS       DAWHSK010000210         3326 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.210, whole genome shotgun sequence.
ACCESSION   DAWHSK010000210 DAWHSK010000000
VERSION     DAWHSK010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3326
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.210"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..718
                     /gene="hisD"
                     /locus_tag="WIC03_001772"
     CDS             <1..718
                     /gene="hisD"
                     /locus_tag="WIC03_001772"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEZ3884237.1"
                     /translation="AVAALAYGTETIPQVDKITGPGNAFVAAAKRRVFGVVGIDMVAG
                     PSEILVIADGTTPADWVAMDLFSQAEHDEIAQAILIGTSQAYLDEVEAAMDRLIETMP
                     RRDIIEASLGNRGAMILAKDLDEACEIANYISPEHLELSVENPQEWAKKIRHAGAIFM
                     GRYTGESLGDYCAGPNHVLPTSRTARFSSPLGTYDFQKRSSLIQVSEQGAQKLGETAS
                     VLAHGESLTAHARAAEFRMK"
     gene            764..1861
                     /locus_tag="WIC03_001773"
     CDS             764..1861
                     /locus_tag="WIC03_001773"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEZ3884238.1"
                     /translation="MKSVRSFIRDDIQAMSAYQIADVPPGFAKLDSMESPVHPFAGHE
                     TLLQEWQARLAAAPIHLYPNPSGSGLQEALRSAFDIPDCADIALGNGSDELIQFITML
                     TAKPGAAMLAAEPSFVMYRHNAALYGMDYVGVPLNGDFTLNLPAVLEAVRKHRPALTF
                     IAYPNNPTGVCFTRAEIEAVIEASDGIVVVDEAYGAFNGDSFLPQAGSIPNLIVMRTV
                     SKIGFAGLRIGYAAGCPEVIGELQKILPPYNMNQLSLTTAKLALQHYGIISANIDSLK
                     NERERMFAELGKICRLNTFPSQANFITIRVPDADLLFDTLKQNRILVKKLHGAHPLLE
                     HCLRITVGSPAQNDAVLNIIRQLYCQPTDFL"
     gene            1858..2775
                     /gene="hisB"
                     /locus_tag="WIC03_001774"
     CDS             1858..2775
                     /gene="hisB"
                     /locus_tag="WIC03_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEZ3884239.1"
                     /translation="MNLTKTQRQLHNFLTLAQEAGSLSKLAKLCGYRTPVALYKLKQR
                     LEKQAEDPDARGIRPSLMAKLEKHTGKPKGWLDRKHRERTVPETAAESTGTAETQIAE
                     TASAAGCRSVTVNRNTCETQITVSINLDGSGKSRLDTGVPFLEHMIDQIARHGMIDID
                     ISCKGDLHIDDHHTAEDIGITLGQAIRQALGDKKGIRRYGHSYVPLDEALSRVVIDLS
                     GRPGLVYNIEFTRALIGRFDVDLFEEFFHGIVNHSMMTLHIDNLSGKNAHHQAETVFK
                     AFGRALRMAVEHDPRMAGQTPSTKGTLTA"
     gene            2870..>3326
                     /locus_tag="WIC03_001775"
     CDS             2870..>3326
                     /locus_tag="WIC03_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEZ3884240.1"
                     /translation="MSANEYTQIGWIGLGQMGLPMVTRLLDGGIEVGVYNRSPDKTAP
                     ISAKGAKVYGNTAELVRDYPVIFLMVSDYAAVCDILNGVRDGLAGKIIVNMSTISPTE
                     NLAVKALVEAAGGQFAEAPVSGSVGPATNGTLLILFGGSEAVLNPLQKIF"
BASE COUNT          844 a         1104 c          788 g          590 t
ORIGIN      
        1 ggcggttgcc gccctcgcct acggcacgga aaccatcccc caagtcgata aaatcaccgg
       61 tccaggcaac gccttcgtcg ccgccgccaa acgccgcgtg ttcggcgtgg tcggcatcga
      121 catggtggcg gggccgtctg aaatcctggt catcgccgac ggcacgacac ctgccgattg
      181 ggtggcgatg gatttgttca gccaggccga acacgacgaa attgcccaag ccatcttaat
      241 cggcacgtcg caagcgtatc tcgacgaagt agaagccgct atggaccgcc tgatcgaaac
      301 tatgccgcgc cgcgacatca tcgaagcctc gctcggcaac aggggcgcga tgatactcgc
      361 caaagacttg gacgaagcct gcgaaatcgc caactacatt tcccccgaac acttggaact
      421 gtcagtcgaa aatccgcagg aatgggcgaa aaaaatccgc cacgccggtg cgattttcat
      481 gggacgctac accggcgaaa gcctcggcga ctactgcgcc ggtccaaacc atgtattgcc
      541 caccagccga accgcccgct tttcctcgcc tttggggaca tatgatttcc aaaaacgctc
      601 cagcctgatt caggtttcgg aacagggcgc gcaaaaatta ggcgaaaccg ccagcgtgct
      661 ggcacacggc gaaagcctga ccgcccacgc ccgcgcggca gagttccgta tgaaataatg
      721 ccgaaacggc gtacaggcat attccaacca ttaaggaaac acgatgaaat ccgtccgctc
      781 cttcatccgc gacgacatac aagctatgtc ggcatatcag attgccgacg ttccgcccgg
      841 ctttgccaaa ctcgattcga tggaaagtcc cgtccaccct tttgccggac atgaaacgct
      901 gttgcaggaa tggcaggcac ggcttgccgc cgcgcccatc catctttacc ccaatccctc
      961 cggcagcggt ttacaggaag cattacgttc ggcgttcgac attcccgact gcgccgacat
     1021 cgcgctgggc aacggctcgg acgagctgat acagttcatc acgatgctga ccgccaaacc
     1081 gggcgcggca atgttggcag ccgaacccag tttcgtcatg taccgccaca acgccgcgct
     1141 gtacggcatg gattatgtcg gcgttccact gaacggagat ttcaccctca acctgcccgc
     1201 cgtcctcgaa gccgtcagga agcaccgccc tgccctgacc tttatcgcct accccaacaa
     1261 ccccaccggc gtatgtttca cgcgtgccga aatcgaagcc gtcatcgaag cttcagacgg
     1321 cattgtcgtc gtcgacgaag cctacggcgc attcaacggc gacagcttcc tgccgcaggc
     1381 gggcagcatt cccaacctca tcgtcatgcg taccgtcagc aaaatcggtt ttgccggact
     1441 gcgtatcggc tatgcggcag gctgtcccga agtcatcggc gaactgcaaa aaatcctgcc
     1501 gccctacaat atgaaccaac tgagcctgac caccgccaaa ctcgccctgc aacactacgg
     1561 catcatctct gccaacatcg acagcctgaa aaacgaacgc gaacggatgt tcgccgaatt
     1621 gggcaaaata tgccgtctga acacctttcc aagtcaggca aacttcatta ccatacgcgt
     1681 acccgatgcc gatttgttgt ttgacacgct caaacaaaac cgcatcttgg ttaaaaaact
     1741 gcatggcgcg cacccgcttt tggaacactg cctgcgcatt accgtaggca gccccgcaca
     1801 aaacgatgcc gttctcaaca tcattcgcca actttactgc caaccaacgg atttcctatg
     1861 aatttgacta aaacacaacg ccaactgcac aactttctga ccctcgccca agaagcaggt
     1921 tcgctgtcca agctcgccaa actctgcggc taccgtaccc ccgtcgcact ctacaaactc
     1981 aaacaacgcc ttgaaaagca ggcagaagac ccagatgcac gcggcatccg tcccagcctg
     2041 atggcaaaac tcgaaaaaca caccggcaaa cccaaaggct ggctcgacag aaaacaccgc
     2101 gaacgcactg tccccgaaac cgccgcagaa agcaccggaa ctgccgaaac ccaaattgcc
     2161 gaaaccgcat ctgctgccgg ctgccgcagc gttaccgtca accgcaatac ctgcgaaacc
     2221 caaatcaccg tctccatcaa cctcgacggc agcggcaaaa gcaggctgga taccggcgta
     2281 cccttcctcg aacacatgat cgatcaaatc gcccgccacg gcatgattga catcgacatc
     2341 agctgcaaag gcgacctgca catcgacgac caccacaccg ccgaagacat cggcatcaca
     2401 ctcggacaag caatccggca ggcactcggc gacaaaaaag gcatccgccg ttacggacat
     2461 tcctacgtcc cgctcgacga agccctcagc cgcgtcgtca tcgacctttc cggccgcccc
     2521 ggactcgtgt acaacatcga atttacccgc gcactaatcg gacgtttcga tgtcgatttg
     2581 tttgaagaat ttttccacgg catcgtcaac cacagtatga tgaccctgca catcgacaac
     2641 ctcagcggca aaaacgccca ccatcaggcg gaaaccgtat tcaaagcctt cgggcgcgcc
     2701 ctgcgtatgg cagtcgaaca cgacccgcgc atggcaggac agaccccctc gaccaaaggc
     2761 acgctgaccg cataaaaaac cataccgtct gaaacacccg caggcttttc agacggtatc
     2821 ggaacagata agattacact acactacaaa cagaaaagga gtaaacatca tgtccgcaaa
     2881 cgaatacaca caaatcggct ggataggctt agggcaaatg ggtctgccta tggtaacgcg
     2941 gctcttggac ggcggcatcg aagtcggcgt atacaaccgc tcgcccgaca aaactgcccc
     3001 catctccgcc aaaggcgcaa aagtttacgg caacaccgcc gaactcgtcc gcgactatcc
     3061 cgtcattttc ctgatggttt ccgactatgc cgccgtgtgc gacatcctga acggagtccg
     3121 cgacggattg gccggcaaaa tcatcgtcaa catgagcacc atctccccga ccgaaaacct
     3181 cgccgtcaaa gcacttgtcg aagccgcagg cggacagttt gccgaagcac ccgtttccgg
     3241 atcggtcggg cccgccacca acggcacgct gctgattctg ttcggcggca gcgaagccgt
     3301 tttaaacccg ctgcaaaaaa tatttt
//
LOCUS       DAWHSK010000211         3322 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.211, whole genome shotgun sequence.
ACCESSION   DAWHSK010000211 DAWHSK010000000
VERSION     DAWHSK010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.211"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(61..849)
                     /locus_tag="WIC03_001776"
     CDS             complement(61..849)
                     /locus_tag="WIC03_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEZ3884241.1"
                     /translation="MNPKIVFFDIDDTLYRKYTDTLRPSVKTAVAALRGKGILTALAT
                     GRSLATIPEKVRDMMAETGMDAVVTINGQFALLHGKTVREVPMDAGSMGRVCAHLDGL
                     GMDYAFVGGEGIAVSALSECVCRALKHIASDFFADKDYFSSKPVYQMLVFAEENEMPL
                     WSDIVEQEGLKTVRWHEEAVDLLPAGMSKTDGIRSVVEALGLEMADVMAFGDGLNDVE
                     MLSEVGFGVAMGNGEQAAKEAAKYVCPSVDEDGVLRGLQDLGVI"
     gene            1174..2529
                     /gene="yegQ"
                     /locus_tag="WIC03_001777"
     CDS             1174..2529
                     /gene="yegQ"
                     /locus_tag="WIC03_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HEZ3884242.1"
                     /translation="MKAPELLLPAGGLERMRAAYDYGADAVYAGSPRYSLRARNNEFA
                     KLDVLEQGIKEAHDRNKKFFLTVNTLPHNSKLKTFVSDMEPLIAMKPDALIMADPGLI
                     MTVREKWPEMPIHLSVQANTTNYWGVKFWQNIGVERIILSRELSMEEIAEIRQQCPDI
                     ELEVFIHGALCIAYSGRCLLSGYFNHRDPNQGTCTNSCRWDYKVHNATESDAGDAQLL
                     QGFNFEKAQEEANQNFEGINGQKRHPYADKVFLIEESNRPGEMMPIMEDEHGTYIMNS
                     KDLRGIEVVEKLAKIGVDSLKVEGRTKSLYYVARVAQSYRKAIDDAVAGRPFDYSLLS
                     ELEGLANRGYTSGFLERHHTQDYQNYLTGHSTAKQSQYVGHVTEIDENGWATVEVKNR
                     FTVGDSLEIIHPSGNQTIKLEQMTRKGQPVDVAPGNGIQVKIPNMQGKEKALIARVLN
                     P"
     gene            2663..3139
                     /locus_tag="WIC03_001778"
     CDS             2663..3139
                     /locus_tag="WIC03_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease"
                     /protein_id="HEZ3884243.1"
                     /translation="MLAACQSGSAPKNAHTASADTPAGESCKVIRGDTAGSGKNIIYR
                     CSNGQAAINAATAASVLESGIPVSFGGGGVPVANGRNLVTRQAANGVGKSDSEACEHA
                     LINAARKFQQTAGKLGGRRVTGFHSYFDKQPLQGGQYDCQAGSFHVRVVMRANVVR"
BASE COUNT          896 a         1017 c          736 g          673 t
ORIGIN      
        1 tgcatgaagg gctgaaaacc gaccctgccg tctgaaaccg gcagggttgt tattatgcgt
       61 tcaaatcacg cccaaatctt gcaagcccct caacacgccg tcttcatcaa cgctggggca
      121 aacatatttc gccgcttctt tcgccgcctg ttccccgttg cccattgcca cgccgaaccc
      181 gacttcagac agcatttcca catcgttcaa accgtcgccg aacgccatca cgtctgccat
      241 ttccaaaccc aatgcttcaa ccacgcttct gatgccgtct gttttcgaca ttcccgcagg
      301 cagcagatcg accgcttcct cgtgccagcg caccgttttc aagccttcct gttccacaat
      361 atccgaccag agcggcattt cgttttcctc cgcaaacacc agcatctgat acaccggttt
      421 gcttgagaaa taatccttat cggcaaaaaa atcgctggcg atatgcttca aggcgcggca
      481 cacgcattcc gacagcgcgg acacggcgat cccctctccg ccgacaaacg cataatccat
      541 gcccaagcca tccaaatgcg cgcaaaccct gcccatcgaa ccggcatcca tcggtacttc
      601 gcgcacagtt tttccgtgca gcagcgcaaa ctgcccgttt atcgttacca cggcatccat
      661 tcccgtttcc gccatcatgt ccctgacctt ttcgggaatc gtcgccaaag accgccccgt
      721 tgccaacgcc gtcaatatac ctttgccgcg caaagccgcc accgccgttt tcacagaagg
      781 acgcaaggta tccgtatatt tgcggtacag tgtatcgtca atgtcgaaaa acacgatttt
      841 aggattcatc acattctctc tcgcattcaa actgccgcat tgtatcccaa gcaggcaaat
      901 acttgataaa tccttataaa tttcccgtca aaattgaccg agaacacgaa aaggcggata
      961 atccgcccat cttcaaacac ccttcagacg gcatttgcag caatgccgtc tgaaacattt
     1021 ttacaaagca tacaaatcat gtttcaacac acaggacgac acataaagcg tcgccctata
     1081 tgttgccctg attcggaagg ggttacgccc ctcccaaata aagtctgatt ctaccgccct
     1141 aaagggcggg gtttcaaccg aaaaggaaac acgatgaaag cacccgaact cttattgccc
     1201 gccggcggat tggaaagaat gcgcgccgcc tacgattacg gcgcagatgc cgtttacgcc
     1261 ggcagcccgc gttattccct gcgcgcccgc aataacgaat ttgccaaact cgacgttctc
     1321 gagcaaggca ttaaagaagc gcacgatcgc aacaagaaat tcttcctgac cgtcaacacc
     1381 ctgccgcaca actccaaact caaaaccttc gtttccgata tggagccctt gattgccatg
     1441 aaacccgacg cgctgattat ggcggatccg ggcttgatta tgaccgtgcg cgaaaaatgg
     1501 ccggaaatgc cgatccacct gtccgtacaa gccaacacca ccaactactg gggcgtgaaa
     1561 ttctggcaga atatcggcgt cgaacgcatc atcctgtcgc gcgaattgag tatggaagaa
     1621 atcgccgaaa tccgccaaca atgccccgac atcgaactcg aagtcttcat ccacggcgca
     1681 ttgtgcatcg cctactccgg ccgctgcctg ttgtcgggct atttcaacca ccgcgatccc
     1741 aaccaaggca cctgcaccaa ctcctgccgt tgggactaca aagtccacaa cgctacggaa
     1801 agcgatgcgg gcgatgccca gcttctgcaa ggtttcaact ttgaaaaagc ccaagaagaa
     1861 gccaaccaaa actttgaagg catcaacggt caaaaacgcc atccctacgc cgacaaggtt
     1921 ttcctgattg aagaatccaa ccgtccgggc gagatgatgc cgattatgga agacgaacac
     1981 ggcacctaca tcatgaactc caaagacctt cgtggcattg aagtggttga gaaactcgcc
     2041 aaaatcggcg tggacagcct caaagtcgaa ggccgcacca agtcgctcta ttatgtcgca
     2101 cgcgtggctc aatcctaccg caaagcgatt gacgatgccg tcgcaggccg tccgtttgat
     2161 tacagcctgt tgagcgaact cgaaggcctt gccaaccgcg gctacaccag cggcttcctc
     2221 gaacgccacc acactcagga ttatcaaaac tacctgaccg gccattccac cgccaaacaa
     2281 agccaatacg tcggacacgt taccgaaatc gatgaaaacg gctgggcaac agtggaagtc
     2341 aaaaaccgct ttaccgtcgg cgattcgctc gaaatcatcc acccgagcgg caaccaaacc
     2401 atcaaattgg aacaaatgac ccgcaaaggc cagcctgtcg atgtcgcccc gggcaacggc
     2461 attcaggtca aaatccccaa tatgcagggt aaagaaaaag ccctcatcgc acgcgtgttg
     2521 aacccctgag ccacaatgcc gtctgaaacc cgttttcaga cggcattttg ttgaatctta
     2581 cggtacaatg gcaatgattt cccaccgcaa gcaaggatat ccgacatgaa aaaaaatcct
     2641 catactctcc gccgccgccc tgatgctggc agcctgccaa tccggcagcg cacctaaaaa
     2701 cgcgcacacg gcatccgccg acacaccggc cggagaatca tgcaaagtta tccgtggaga
     2761 caccgcagga agcggcaaaa acatcattta ccgttgcagc aacggtcagg cggcaataaa
     2821 tgcagccacc gccgccagcg tattggaatc agggattccc gtcagcttcg gcggcggggg
     2881 cgtaccggtt gcaaacggca ggaatctggt tacacggcaa gcagcaaacg gcgttggcaa
     2941 atccgactcc gaggcctgcg aacacgccct aatcaatgcc gcccgcaaat tccagcagac
     3001 cgcaggcaaa ctcggcgggc gccgtgttac cggcttccac agctacttcg acaagcagcc
     3061 gctgcaaggc ggacaatacg actgtcaggc aggctcgttc cacgtccgcg tggtcatgcg
     3121 cgccaatgtc gtccgttaac atatcggcaa tcaaaaaatt aggctgtgtt ttagtaatct
     3181 gttgatttca attatttgca aaggaaaaga caattatttt tcggttagga agagacctat
     3241 cctattgaat acatttaagt taaatacgtc tatcaacact atattatagt ggattaacaa
     3301 aaatcaggac aaggcgacga ag
//
LOCUS       DAWHSK010000212         3321 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.212, whole genome shotgun sequence.
ACCESSION   DAWHSK010000212 DAWHSK010000000
VERSION     DAWHSK010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3321
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.212"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            56..3187
                     /locus_tag="WIC03_001779"
     CDS             56..3187
                     /locus_tag="WIC03_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEZ3884244.1"
                     /translation="MNLETKPIAETPNFIVLDQYEKIEQSGSYQSENQLEAELIADLQ
                     NQGYEYRKDLNSQSRLLENLRAQLQRLNDVAFSDGEWARFLTEYLDRPSENITDKTRK
                     IHDDHIYDFAFDDGRLKNIYLLDKKNLARNHVQLINQFEQTGTHANRYDVTVLVNGLP
                     LVQIELKKRGVAVREAFNQVHRYSKESFNSENSLFKFLQIFVISNGTDTRYFANTTKR
                     DKNSFDFTMNWARSDNHPIKDLKDFTATFLQKSVLLGVLLHYSVFDANDTLLIMRPYQ
                     IAAAERILWKINSSAQAKNWSGPESGGYVWHTTGSGKTLTSFKAARLATESAFIDKVF
                     FVVDRKDLDYQTMKEYQRFSTDSVNGSESTAGLKRNLEKDDNKIIVTTIQKLNNLMKS
                     EDNLPVYHQQVVFIFDECHRSQFGEAQKNLKKKFKKFCQFGFTGTPIFHDHIVDGKLI
                     MKGNALGSESTESVFGRQLHSYVITDAIRDEKVLKFKVDYNDVRPQFKAVEAEQDEKK
                     LSAAENRQALLHPERIREITQYILNQFRQKTHRLNAGGKGFNAMFAVSSVDAAKCYYE
                     AFKTQQAGSLHPLKVATIFSFAANEEQNAVGEIADETFEPEAMDSSAKEFLQAAINDY
                     NACFKTNFGTDSKAFQNYYRDLAKRVKNQEVDLLIVVGMFLTGFDAPTLNTLFVDKNL
                     RYHGLMQAFSRTNRIYDATKTFGNIVCFRDLEQATIDAITLFGDKNTKNVVLEKSYEE
                     YMNGYTDSQTGEARRGYLDVAKELHERFPDPDKIETEKDKKDFAKLFGEYLRAENVLQ
                     NYDEFAALRELQSVDAADEDAMKAFQEKYYLSDEDVQEMRQVPMPSERAVQDYRSAYN
                     DIRDWLRRQKAGEQKEQSKIDWDDVVFEVDLLKSQEINLDYILQLIFEHHKKIKGKAE
                     LVEEIRRIIRASIGHRAKESLIVDFINDTDLDKVPDVPAILETFYTYAQEVMRNEAAE
                     LIAAEGLNETAAKRYLISSLKRGYASENGTELTETMPKMSPLNPQYLTKKQSVFQKIA
                     AFVEKFAGIGTDI"
BASE COUNT          967 a          799 c          845 g          710 t
ORIGIN      
        1 gtaccggttt aaatttaatt cactataaaa cccgacaccg caaaggacga cccatatgaa
       61 cctcgaaact aaacccatcg ccgaaacgcc gaatttcatc gtgctcgacc aatatgaaaa
      121 aatcgaacag tcgggcagct accaatcgga aaaccagttg gaagcggagt taatcgccga
      181 tttgcagaat cagggttacg aataccgcaa ggatttgaac agccaaagca ggctgctgga
      241 aaacctgcgc gcgcagttgc agcggctgaa cgatgtggcg ttttcagacg gcgaatgggc
      301 gcggtttttg acggaatatc tggacaggcc gtctgaaaac attaccgata aaacccgaaa
      361 aatccacgac gaccatattt acgatttcgc ttttgatgac ggccgtctga aaaatattta
      421 tctgctggac aaaaaaaacc ttgcccgcaa ccatgtgcag cttatcaacc agtttgagca
      481 gacgggcacg catgcaaacc gctatgacgt taccgtgttg gtaaacggct tgccgctggt
      541 gcagattgaa ttgaaaaagc gcggcgtggc ggtgcgcgag gcattcaatc aggtgcaccg
      601 ttacagcaaa gagagcttca acagcgaaaa ttcgctgttc aaattcctgc aaatcttcgt
      661 gatttccaac ggcacggata cgcgctattt cgccaacacc accaagcgcg acaaaaacag
      721 cttcgatttc acgatgaatt gggcgcggtc ggacaatcat ccgattaagg atttgaaaga
      781 ctttactgcc acgttcctgc agaaaagcgt attgctgggc gttttgctgc attacagcgt
      841 gttcgatgcg aatgatacgc tgctgattat gcggccgtac cagattgccg ccgccgaacg
      901 cattttgtgg aaaatcaaca gttcggcgca ggcgaagaat tggagcgggc cggaaagcgg
      961 cggctatgtc tggcacacca cgggcagcgg caaaacgctg accagcttca aggcggcgcg
     1021 tctggcgacg gaatcggcgt ttatcgacaa ggttttcttc gtggtggaca ggaaggattt
     1081 ggactatcag acgatgaagg aataccaacg tttttcgacc gacagcgtga acggttcgga
     1141 aagcacggcg ggcttgaaac gcaatttgga aaaagacgac aacaaaatca tcgttaccac
     1201 catccagaag ctgaacaacc tgatgaagag tgaagataat ctgccggttt atcaccagca
     1261 agttgtcttt attttcgacg aatgccaccg ttcgcaattc ggcgaagcgc aaaaaaacct
     1321 gaaaaagaaa tttaaaaaat tctgccagtt cggttttacc ggtacgccga tttttcatga
     1381 ccatatcgtt gatggcaaac tgattatgaa aggcaatgcg cttggttcgg agagtacgga
     1441 aagcgtattt gggagacagc tgcattctta tgtgattacc gatgccatcc gcgatgaaaa
     1501 agtattgaaa ttcaaagtgg attacaacga tgtgcgcccg cagttcaaag ccgtggaagc
     1561 ggaacaggac gagaagaaac tgagtgccgc cgaaaaccgc caagccctgc tgcatcctga
     1621 acgcatccgc gaaatcacgc aatatatcct gaaccagttc aggcagaaaa cacaccgctt
     1681 gaatgcgggc ggcaaaggct ttaacgccat gtttgccgtc agcagcgtgg atgcggcgaa
     1741 gtgctattac gaagcgttca aaacacaaca ggcaggcagc ctgcacccgc tgaaagtggc
     1801 caccattttt tcctttgccg ccaacgaaga gcaaaacgcc gtcggtgaaa ttgccgatga
     1861 aacctttgaa ccggaagcga tggacagcag cgcaaaagaa tttttgcagg ctgccatcaa
     1921 cgattacaac gcctgtttca aaaccaattt cggcacggac agcaaagcct ttcagaacta
     1981 ctaccgcgat ttggcaaaac gggtaaaaaa tcaggaagtg gatttgctga ttgtggtcgg
     2041 catgtttttg acgggttttg acgcgccgac gctgaacacg ctgtttgtcg ataaaaacct
     2101 gcgctatcac ggcctgatgc aggcgttttc gcgcaccaac cgcatttacg atgccaccaa
     2161 aaccttcggc aacattgtct gcttccgcga tttggagcag gcaaccattg atgcgattac
     2221 cttgtttggc gacaaaaaca ccaaaaatgt agtgctggaa aaaagttacg aagaatacat
     2281 gaacggctat accgacagcc agaccggcga agcgcgacgc ggctatttgg atgtggcaaa
     2341 agaattgcac gagcgtttcc ccgatcccga caaaatcgaa acggaaaaag acaaaaaaga
     2401 ttttgccaaa ctcttcggcg aatacctgcg ggcggaaaac gtattgcaga actacgatga
     2461 atttgccgcg ctgcgcgagt tgcagagtgt ggacgcggcg gacgaagatg cgatgaaggc
     2521 gtttcaagaa aaatactacc tgagcgatga agacgtgcag gaaatgcggc aagtgccgat
     2581 gccgtctgaa agggcggtgc aggactaccg ttccgcctac aacgacatcc gcgattggtt
     2641 gcgccgccaa aaagcaggcg aacagaaaga acagtcaaaa atcgactggg acgatgtggt
     2701 ttttgaggtg gatttgctca aatcgcagga aatcaatctg gattacatct tgcaactgat
     2761 tttcgagcat cacaaaaaaa tcaaaggcaa agcggagctg gtggaagaaa tccgccgtat
     2821 catccgcgcc agtatcggac accgcgccaa agagagcctg attgtggatt tcatcaacga
     2881 tacggatttg gataaagtcc ccgatgttcc cgccatactg gaaaccttct acacctacgc
     2941 tcaagaggtg atgaggaatg aagccgcaga gctgattgcc gccgaaggtt tgaacgaaac
     3001 cgccgccaaa cgctatttaa tcagctcgct caaacgcggc tatgccagcg aaaacggtac
     3061 ggaactgacc gaaaccatgc cgaaaatgag tccgctcaac ccgcaatatc tgacgaagaa
     3121 acaaagtgtt tttcaaaaga ttgcggcgtt tgtggagaag tttgccggaa tagggacaga
     3181 tatttgacaa aatgccgtct gaacctttca gacggcattt ttgattttat gcggaggcgg
     3241 tttttatttt gaccttgctt ttcttaaact tcaacacggc ttcttctttt gccgcatccc
     3301 agtctatccg cacgaagccg c
//
LOCUS       DAWHSK010000213         3316 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.213, whole genome shotgun sequence.
ACCESSION   DAWHSK010000213 DAWHSK010000000
VERSION     DAWHSK010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.213"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..491)
                     /locus_tag="WIC03_001780"
     CDS             complement(<1..491)
                     /locus_tag="WIC03_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEZ3884245.1"
                     /translation="MKKLNTQSPDFQAGLKALLAFETAQNPETERIVADICADVQKRG
                     DAALIEYTNKFDQTNAKSIDDLILTQADLKAAFERIPNDVQTALQTAARRVESYHQRQ
                     KMESWSYTDEDGTLLGQQITPLDRVGIYVPGGKAAYPSSVIMNAMPAHVAGVKEIIMV
                     VPTP"
     gene            complement(488..1387)
                     /locus_tag="WIC03_001781"
     CDS             complement(488..1387)
                     /locus_tag="WIC03_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HEZ3884246.1"
                     /translation="MVAKIKKFSDSTLSVLNNGERRFYVYCLTDLKKDKILYIGKGCG
                     NRIFEHEWVASRSQDPVSGEIIDRKLKAISKCKKLGRYIISYHLTEVEALAAESALIH
                     FVKSVLGKKLKNKIAGHGPGGISVEELDRRFGFSSLPLNEINPDGLILAIKIHNAFDL
                     DTDEELDYLFDNQDDANLKSRTLGNWVIGKDVASKVKYVIGVHTGLQNAVVSAYEVDG
                     FETMVEETKNGRKQSRYRFRTTSRSEEVLAKLGLQQKCLPELKFGSGGEKAYIRPKTE
                     TETEQENIQTTPNPKIKKEKTKS"
     gene            complement(1491..2144)
                     /locus_tag="WIC03_001782"
     CDS             complement(1491..2144)
                     /locus_tag="WIC03_001782"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEZ3884247.1"
                     /translation="MQDNALTIALSKGRIFEETLPLLAAAGIVPTEEPEKSRKLIIGT
                     NHENIRLVIVRATDVPTYVRYGAADFGIAGKDVLIEHGGTGLYRPLDLEIAKCRMMVA
                     VRKGFDYEAASQPGCRLKIATKYPEIAASHFAGKGVHVDIIKLYGSMELAPLVGLSDA
                     IVDLVSTGNTLKANGLEAVEHIVDISSRLVVNKAALKTKYALLEPIIQAFGGAVKAK"
     gene            complement(2223..2876)
                     /locus_tag="WIC03_001783"
     CDS             complement(2223..2876)
                     /locus_tag="WIC03_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEZ3884248.1"
                     /translation="MFSVPRSFLPGVFVLAALAACKPQDNSAAQAASSSASASAAENA
                     AKPQTRGTDMRKEDIGGDFTLTDGEGKPFNLSDLKGKVVILSFGFTHCPDVCPTELLT
                     YSDTLKQLGGQAKDVKVVFVSIDPERDTPEIIGKYAKQFNPDFIGLTATGGQNLPVIK
                     QQYRVVSAKVNQKDDSENYLVDHSSGAYLIDKNGEVAIFSPYGSEPETIAADVRTLL"
     gene            3153..3237
                     /locus_tag="WIC03_001784"
     tRNA            3153..3237
                     /locus_tag="WIC03_001784"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3187..3189,aa:Leu,seq:caa)
BASE COUNT          788 a          795 c          831 g          902 t
ORIGIN      
        1 ggtgtcggca cgaccatgat gatttctttc acacctgcga cgtgggcggg catggcgttc
       61 atgatgacgg aactcggata cgccgccttg ccgccgggga cgtaaatgcc gacgcggtca
      121 agcggtgtga tttgttgtcc caacagcgtg ccgtcttcat cggtgtagct ccacgattcc
      181 attttttggc gttggtggta gctttcgaca cggcgggcgg cggtctgcaa tgccgtctga
      241 acgtcgttcg gaatgcgctc gaacgccgct ttcaaatcgg cttgcgtgag tattaaatca
      301 tcgatgcttt tagcgtttgt ctgatcgaat ttgttggtgt attcaatcaa agccgcatcg
      361 ccgcgctttt gcacatcggc gcaaatgtcg gcgacgatgc gttcggtttc ggggttttgc
      421 gcggtttcaa aagccagcag ggctttgagt ccggcttgga aatcgggcga ttgggtgttg
      481 agttttttca tgatttggtt ttttcctttt ttatttttgg attgggggtc gtctgaatat
      541 tctcttgttc agtttctgtc tctgttttgg gtctgatata cgctttttct cccccgctac
      601 caaacttcaa ttcgggcagg catttttgtt gcagaccgag tttggctaat acctcttcgc
      661 tacgagaggt agtgcggaaa cggtaacggg attgttttct accgtttttg gtttcctcaa
      721 ccattgtttc aaaaccgtcc acttcgtatg cactgacaac agcgttttgc agaccggtgt
      781 gaacgccgat aacgtatttc acttttgaag caacatcttt accgataacc cagttgccca
      841 acgtacgcga tttgaggttg gcatcgtctt ggttgtcgaa aaggtagtct aattcttcgt
      901 cagtatctaa atcgaaagca ttgtggattt tgatggcgag aatcagcccg tcggggttaa
      961 tctcgttaag tgggagagaa gagaatccaa agcggcgatc tagttcttct acgctaatac
     1021 cacccggacc atgcccggca attttatttt tgagtttttt acccaagaca gatttaacaa
     1081 aatgaattaa ggcagattcg gcggcgagtg cttcgacttc agtcagatga tagctgatga
     1141 tatagcggcc gagtttcttg catttggaga tggctttgag tttccgatcg ataatctcgc
     1201 cggagactgg gtcttgtgaa cgactagcaa cccattcatg ctcgaagata cgattaccgc
     1261 agcctttgcc gatgtagagg attttgtctt ttttcaggtc ggtcagacaa tagacataaa
     1321 accgacgctc gccgttattc aaaacggaaa gggttgaatc tgagaatttt tttatttttg
     1381 caaccatttt aacttctcct atacagttta ctgccggtga tattttcaga cgacctgtcg
     1441 gcgggaacgg atagggtcgt ctgaaaacgc atctttattc aaatggatgc ttacttcgcc
     1501 ttcactgcgc cgccgaacgc ctgaataatc ggctccagca gcgcgtattt cgttttcaaa
     1561 gcagccttgt tgaccaccag gcggctggaa atgtcgacga tgtgttcgac tgcttccaag
     1621 ccgtttgcct tcaaggtgtt gcccgtcgaa accaagtcca caatcgcatc gctcaagccg
     1681 accagcggcg caagttccat cgagccgtac agtttgataa tgtccacatg gacacccttg
     1741 ccggcaaaat gagatgccgc gatttcagga tacttcgtgg caatcttcag acggcatccg
     1801 ggttgcgaag ctgcttcgta atcaaaccct ttacgcacag caaccatcat gcggcacttg
     1861 gcaatctcca aatccaaagg ccggtaaagc cccgtgccgc cgtgttcgat cagcacgtct
     1921 ttgcccgcaa tgccgaagtc cgccgcgccg tagcggacat aagtcggcac atcagttgcg
     1981 cggacaatga caaggcggat gttttcatgg ttcgtcccga taatcagctt gcgcgatttt
     2041 tcaggctctt cagtcggaac aatgccggca gcggcaagca gcggcagcgt ctcctcaaaa
     2101 atgcgcccct tggataaggc gatggtcaaa gcattatcct gcatggtgtg gatgtccttt
     2161 gtcggttgaa acaataatgc cgtctgaata ggcgccgacg gtgcagacgg catacggttt
     2221 tatcagagca gagtccttac atcggcagca atcgtttccg gctcgcttcc gtaaggcgag
     2281 aaaatggcaa cctcaccgtt tttgtcgatg agatacgcac cggaagagtg gtcgaccaaa
     2341 tagttttcgc tgtcgtcttt ttgattgact ttggcagaaa ccacgcggta ttgctgcttg
     2401 atgaccggca ggttttggcc gcccgttgcc gtcagaccga taaagtccgg attgaactgt
     2461 ttggcatact tgccgatgat ttcaggcgtg tcgcgttccg gatcgatgct gacgaacacc
     2521 actttcacgt ccttagcctg cccgcccaac tgcttcaacg tgtcgctgta cgtcaaaagc
     2581 tctgtcgggc agacatcggg acagtgcgta aagccgaaag acagaatcac gaccttgcct
     2641 ttcaaatcgc tcaggttgaa aggcttgcct tcgccgtcgg tcagcgtgaa atcgccgccg
     2701 atgtcttcct tacgcatatc cgtaccgcgc gtttgcggct ttgccgcatt ttccgcagcc
     2761 gacgcggatg cacttgaaga agcggcttgc gccgcactgt tgtcttgagg tttgcaggcg
     2821 gcaagcgcgg caagtacgaa aacgcccggc aaaaaggaac gcggtacgga aaacatgaca
     2881 atctcccaag aataaatagc aaaacctgat tcgtacggaa tttgaatatt tccatcttaa
     2941 cgcgtttggc agatttaatg tagtattttt ttacgttgtc tgcacggcag gaatagccga
     3001 atcatacaga atctttggag cggaaagaac agtaagatta aaataataaa aattctattt
     3061 aaaatcaaaa ataaaataaa aacagataaa ataatttcaa aaaaaaggtt tacagaattt
     3121 caaaagccat tataatgacg acttcttcac cagcccaggt ggcgaaattg gtagacgcag
     3181 gggactcaaa atcccccgcc gcaaggtgtg tcggttcgag tccgaccctg ggcaccacaa
     3241 ccgcttttag agcggttatt tttttgtcta ttgctgttta atgaaataga taagactaaa
     3301 tatagtggat taaatt
//
LOCUS       DAWHSK010000214         3314 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.214, whole genome shotgun sequence.
ACCESSION   DAWHSK010000214 DAWHSK010000000
VERSION     DAWHSK010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3314
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.214"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(149..967)
                     /gene="cysE"
                     /locus_tag="WIC03_001785"
     CDS             complement(149..967)
                     /gene="cysE"
                     /locus_tag="WIC03_001785"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEZ3884249.1"
                     /translation="MKKDHLNTTGFDLWHTIREETAAAVSAEPMLASFLHQTVLRHES
                     LGSVLAYHLSSKLGSPIMDVRALFEIYQQALGSDTQIGKCVEADLKAIYERDPACDEY
                     SLPLLYFKGFHAIQAHRINHRLYLDGRKTLAYFLQNRMSEVFGVDIHPAARFGYGLML
                     DHATGFVAGETAVLGNNISILHGVTLGGSGKEGGDRHPKIGDGVMIGANASILGNIRI
                     GSNAKIGAGSVVVSDVPPSITVVGVPAKPVARSLKTPSADMDQNIQFTEIDFMI"
     gene            1151..1729
                     /gene="grpE"
                     /locus_tag="WIC03_001786"
     CDS             1151..1729
                     /gene="grpE"
                     /locus_tag="WIC03_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEZ3884250.1"
                     /translation="MSEQTQQQNSEEAVENVEAVETVETVGNADGVQEQAAAEPAYED
                     LQARIAELEAQLKDEQLRALANEQNLRRRHQQEIADTHKFAGQKFAVEMLPVKDYLEM
                     ALLDQSGNFDALKMGVQMTLNELQKAFDATQIKEINPKAGDKLDPNIHQAMQAVASEQ
                     EPNTVVGVMKKGYTLSDRVLRPAMVTVAQKEA"
     gene            complement(1853..2068)
                     /gene="nqrM"
                     /locus_tag="WIC03_001787"
     CDS             complement(1853..2068)
                     /gene="nqrM"
                     /locus_tag="WIC03_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Na+)-NQR maturation NqrM"
                     /protein_id="HEZ3884251.1"
                     /translation="MKTLLLTFGIFLTVIIGMAVGYIFSKRSIKGSCGGITALGMKKM
                     CDCDTPCDTLQKKLDEEKQAGGIRVDR"
     gene            complement(2094..3149)
                     /locus_tag="WIC03_001788"
     CDS             complement(2094..3149)
                     /locus_tag="WIC03_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEZ3884252.1"
                     /translation="MPSETRLPNFIRVLIFALGFIFLNACSEQTAQTVTLQGETMGTT
                     YTVKYLSNNRDKLPSPAEIQKRIDDALKEVNRQMSTYQPDSEISRFNQHTAGKPLRIS
                     SDFAYVTAEAVRLNRLTHGALDVTVGPLVNLWGFGPDKSVTREPSPEQIKQAASYTGI
                     DKIILKQGKDYASLSKTHPKAYLDLSSIAKGFGVDKVAGELEKYGIQNYLVEIGGELH
                     GKGKNARGEPWRIGIEQPNIVQGGNTQIIVPLNNRSLATSGDYRIFHVDKNGKRLSHI
                     INPNNKRPISHNLASISVVADSAMTADGLSTGLFVLGETEALKLAEREKLAVFLIVRD
                     KGGYRTAMSSEFEKLLR"
BASE COUNT          732 a          747 c         1001 g          834 t
ORIGIN      
        1 cggttgcgtg ggaatcgggt gtatcgaata aaaggcattt tgtccgactg gcaagtgccg
       61 gcatcggcgg catatcaagg cgcaggcttg aagcgggcaa tgccgtctga agcacgtttg
      121 gcgtttcaga cggcattggt gcggatattc aaatcataaa gtcgatttcg gtaaactgga
      181 tattttgatc catatccgcc gacggtgttt tgagcgatcg cgccacgggt ttggcgggta
      241 cgccgacaac cgtgatggac ggcggcacgt ctgaaaccac gacgctgccc gccccgattt
      301 tggcattgct gccgatgcgg atattgccca atatcgaggc gtttgcgccg atcatcacgc
      361 cgtcgccgat tttagggtgg cggtcgccgc cttctttgcc cgaaccgccg agcgttacgc
      421 cgtgcaaaat cgaaatattg ttgcccaaca cggcggtttc gccggcaaca aagccggtgg
      481 cgtggtcgag catcagcccg tatccgaaac gggcggcggg atggatgtcc acgccgaata
      541 cttcggacat acggttttgc aggaaatacg ccagcgtttt gcgcccgtcg agatacagcc
      601 ggtggttgat gcggtgtgcc tgaatcgcgt ggaagccttt gaaatataaa agcggcagcg
      661 aatattcgtc gcaggcggga tcgcgttcgt agatggcttt taagtctgct tcgacgcatt
      721 tgccgatttg ggtgtcgctg cccaacgcct gctggtagat ttcaaacagc gcgcgcacgt
      781 ccataatcgg gctgccgagt ttgctggaaa ggtggtaggc aaggacggag ccgagggact
      841 cgtggcgcaa cacggtttgg tgcaaaaaac ttgccagcat cggttcggcg gagaccgcgg
      901 ccgcggtttc ttcgcggatg gtgtgccaga ggtcgaaacc ggttgtgttt aaatggtctt
      961 ttttcatgag tgatgacgtt tgaaaatcga tatggtcggc agtatcttac cgtctatatt
     1021 attttttcgg taggggattt gaaaatgaat ttgaaattct ctgcttttgc ttgaagtttc
     1081 ttgaaaatgt ccttatcttg cgcgggtaat aactggattt tgatttccaa tttgttttaa
     1141 gggatacgat atgagcgaac agacacagca gcaaaacagt gaagaagcgg ttgaaaatgt
     1201 ggaggcggtg gaaaccgtcg agacagtagg aaatgcggac ggtgtgcagg aacaggctgc
     1261 cgcagagccg gcttatgagg atttgcaggc gcggattgcc gagctggaag cgcagttgaa
     1321 agacgagcag ctgcgcgctt tggcaaacga gcaaaacctg cgccgccgcc accagcagga
     1381 aattgcggat acgcacaagt tcgccggaca gaagtttgcc gtggaaatgc tgccggtcaa
     1441 ggattatctg gaaatggcgc ttttggatca gagcggcaat ttcgatgcgc tgaaaatggg
     1501 cgtgcagatg actttgaacg agttgcagaa agcatttgat gctacgcaaa tcaaggaaat
     1561 caaccctaaa gcgggcgata agctcgatcc gaatatccat caggcgatgc aggcggtggc
     1621 aagcgaacag gagccgaata ccgtggtggg tgtgatgaag aagggttata cgctgtccga
     1681 ccgcgtgttg cgcccggcta tggttacggt ggcacagaag gaagcctgaa ggcgtctggg
     1741 gaataatctg atttatttcc tgaagcgcgt tttgcgtata aaccgatcga agtaaagcgg
     1801 caatgccgtc tgaacccgcc tgtcgggctt cagacggcat tttgtttcgg tgttaacggt
     1861 caacccgtat gccgcctgcc tgtttttctt catccagttt cttttgcagg gtgtcgcaag
     1921 gtgtgtcgca gtcgcacatt tttttcatac ccaaggcagt aatgccgccg caactgcctt
     1981 tgatgctgcg tttggagaaa atatagccga ccgccatacc gatgatgacg gtcaggaaga
     2041 tgccgaaggt aaggagcagg gttttcatgg tgtttcctaa tcggtttgta tgtttagcgg
     2101 agcagttttt caaattcgga agacatggcg gtgcggtagc cgcctttatc cctgacaatc
     2161 aggaaaacag cgagtttttc gcgctctgcc agctttaagg cttcggtttc gcccaatacg
     2221 aataatcctg tggacaagcc gtccgccgtc atcgcactgt ctgcgaccac gctgatggag
     2281 gcgaggttgt ggctgatggg tcgtttgttg ttcgggttga tgatatggga gaggcgtttg
     2341 ccgtttttat cgacgtggaa aatacggtaa tcgccggaag tggcaagcga acggttgttc
     2401 agcgggacga taatctgcgt attgccgcct tggacgatat tgggctgctc gataccgatg
     2461 cgccacggtt cgccgcgcgc gtttttgcct ttgccgtgca actcgccgcc gatttcgacc
     2521 agataatttt gaatgccgta tttttccagt tcgcccgcaa ctttatcaac gccgaagcct
     2581 ttggcaatcg aagataaatc caaataggcc ttggggtggg ttttgctcaa ggaagcgtaa
     2641 tctttgcctt gtttcaaaat gattttgtct atgcccgtat aagatgccgc ctgtttgatt
     2701 tgttccggcg acggttcacg ggtaacggat ttgtcggggc cgaatcccca aaggttgacc
     2761 aaagggccga cggttacgtc cagcgcgccg tgtgtcaggc ggtttaggcg gacggcttcg
     2821 gcagtaacgt atgcgaagtc gcttgaaatg cggaggggct tgccggctgt gtgttggttg
     2881 aaccggctga tttcggagtc gggctgatag gtggacatct gccggttgac ttctttaagc
     2941 gcgtcatcga tgcgcttttg tatttcggca ggtgagggga gtttgtcccg attatttgaa
     3001 aggtatttga cggtataggt cgtgcccatc gtttcgcctt gcagggtaac ggtttgcgcg
     3061 gtttgttccg aacaggcgtt caggaagatg aaacccaggg caaatatcaa gacgcggata
     3121 aagttcggca ggcgtgtttc agacggcata gtgtttgacg gttttggcaa atggtttgaa
     3181 ttatatcgca aaacggccga tatgtttcta tgccgatgcc gtctgaaggg catcaggggc
     3241 atcgcccgct gtttgggggc ggtaataaaa aaacaaaccg gtgttttaca aaccgagacc
     3301 tttgcaaaat tcct
//
LOCUS       DAWHSK010000215         3270 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.215, whole genome shotgun sequence.
ACCESSION   DAWHSK010000215 DAWHSK010000000
VERSION     DAWHSK010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3270
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.215"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(347..934)
                     /locus_tag="WIC03_001789"
     CDS             complement(347..934)
                     /locus_tag="WIC03_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEZ3884253.1"
                     /translation="MNNMFAAKLSKLVYTASDHPDSLSEMEEFDRLILLIRKLYQILD
                     GQHILSRVTVCLHHQNRRGLIALDKAAAGCDAAMLRAQRWRIYTQIAERLSGSGGGFT
                     VTAESVSAACPELEGRYLELVRRAAVSFGFTQGWENPAGGQEKTQAVPDAASGHSVRK
                     PIKINIEAKVERYPAYRPTAALKVTRRRFFRLQNR"
     gene            1147..1344
                     /gene="iscX"
                     /locus_tag="WIC03_001790"
     CDS             1147..1344
                     /gene="iscX"
                     /locus_tag="WIC03_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HEZ3884254.1"
                     /translation="MKWTDTRRIAEELYDLHGETIDPRTVRFTQLRDLIMALPEFDDD
                     PARCGERILESVQQAWIDEAE"
     gene            complement(1412..1813)
                     /locus_tag="WIC03_001791"
     CDS             complement(1412..1813)
                     /locus_tag="WIC03_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEZ3884255.1"
                     /translation="MKDKHDSSAMRLDKWLWAARFFKTRSLAQKHIELGRVQVNGSKV
                     KNSKTIDIGDIIDLTLNSLPYKIKVKGLNHQRRPASEARLLYEEDAKTAALREERKQL
                     DQFSRITSAYPDGRPTKRDRRQLDRLKKGDW"
     gene            2024..2983
                     /locus_tag="WIC03_001792"
     CDS             2024..2983
                     /locus_tag="WIC03_001792"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEZ3884256.1"
                     /translation="MKPVFLDFEQPIAELTNKIDELRFVQDESAVDISDEIHRLQKKS
                     NDLTKSIFSKLTPAQVSQVSRHPQRPYTLDYIDALFTDFEELHGDRHFADDHAIVGGL
                     ARFNGQSVMVVGHQKGRDTKEKIRRNFGMPRPEGYRKALRLMKTAEKFGLPVMTFIDT
                     PGAYPGIGAEERGQSEAIGKNLYELTRLHVPVLCTVIGEGGSGGALAVAVGDYVNMLQ
                     YSTYSVISPEGCASILWKTAEKAADAAQALGITADRLQKLDLVDTVIKEPLGGAHRDF
                     GQTMKNVKAVLEKQLHEAQSIPLADLLSRRFDRIMAYGKFSEQ"
     gene            3051..>3270
                     /locus_tag="WIC03_001793"
     CDS             3051..>3270
                     /locus_tag="WIC03_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(Ile)-lysidine synthetase"
                     /protein_id="HEZ3884257.1"
                     /translation="MLTLDAFEQCLKDCFPQGLNGKKTAVALSGGLDSVVLLHLLVRA
                     GKKGGFIPDALHIHHGLSPRADDWADFCQ"
BASE COUNT          767 a          824 c          889 g          790 t
ORIGIN      
        1 gaagatttga ttgtggaaat ccccaaatac accatcaacc acgcgcgcga agaacactga
       61 aaacaggccg tctgaagccg gcacgcttca gacggcattg ttgcgcggat aaggcgcaat
      121 cgcccgaaaa caggcgttcg tacaggcgga actttcgatt ctatagcgaa ttaacaaaaa
      181 tcaggacaag gcggcgagcc gcagacagta caaatagtac ggcaaggcga ggcaacgctg
      241 taccggttta aatttaattc actatatatt gatttttatc ggttttctga cggaacggat
      301 tggtgcggca tccgaggcgg attactcggg cgcgatgcac gggtatttat cggttttgca
      361 gccggaaaaa ccgccggcgg gttactttca gggcggcggt cgggcggtat gccggatagc
      421 gttccacttt cgcttctata ttgattttga ttggttttct gacggaatga cccgatgcgg
      481 catccgggac ggcttgtgtt ttttcctgcc cgcctgccgg attttcccat ccttgcgtga
      541 aaccgaaaga gacggcggcg cggcggacaa gctcgagata gcgtccttca agctccgggc
      601 aggcggcgga cacgctttct gccgtaaccg tgaagccgcc gcccgacccg gaaaggcgtt
      661 ccgcaatctg cgtgtagata cgccaacgtt gggcgcgcaa cattgccgca tcgcaaccgg
      721 cagccgcttt atccaaggca atcaggccgc gccggttttg gtggtgaagg caaaccgtta
      781 ctctggagag gatatgttgc ccgtccaata tttgatacag tttgcgtatc agcagaatca
      841 ggcggtcaaa ctcctccatc tccgacaggg aatcaggatg gtcggaagcg gtataaacca
      901 gtttggacaa ttttgcggca aacatattgt tcatcaatct tccttgtcgg ttgaaacccc
      961 gctcttcggg gcggtagaat cagatttgtt tgggagggac aactcctccc agatcaggac
     1021 gacacatagg ctggtgcttg atgtgttgtc cggcgagttg aaacattcag ccatcctcaa
     1081 ggtgcggcag ttttgccgaa acatattcta cacggcttca atgccggacg ataaaaggaa
     1141 attcatatga aatggaccga cacccggcgt atcgccgaag aactctatga cctgcacggc
     1201 gaaaccatcg atcccagaac cgtgcgcttt acccaactgc gcgacctgat tatggcattg
     1261 cccgaatttg acgacgaccc cgcccgttgc ggcgaacgca tcctcgaatc cgtgcagcag
     1321 gcatggatag acgaggcgga ataagtttcg ggaatgccgt ctgaaatgcg gcggtacgcg
     1381 gttcgtgctt ctgtttgcag cgggaatggt tttaccagtc tccttttttc agcctgtcca
     1441 gttggcggcg gtcgcgcttg gtcggtctgc cgtcgggata ggcggaagtg atgcggctga
     1501 attggtcgag ctgtttgcgc tcttccctca atgctgccgt tttcgcgtcc tcttcataca
     1561 gaagccgcgc ctcggatgcc gggcggcgtt ggtggttcaa acctttaacc ttgattttat
     1621 agggaaggga attgagcgtc aggtcgataa tatcgccgat gtctatggtt ttactgtttt
     1681 tgaccttcga gccgtttact tgaaccctac ccagttcgat gtgcttttgc gcaagggaac
     1741 gggtcttgaa aaaacgtgcc gcccaaagcc atttgtccag ccgcatggcg gaagaatcgt
     1801 gcttgtcttt catacgattt tgtttgaaat aattgaattt gtttcgagtt tagcataaga
     1861 tacgccgcct ataactagta tatatgcact aatccactgt tttccatgct gtccgaacac
     1921 aaaaagaggg tgtggaaaag ccgttttgga caataaatta actgcggaat atgcacaaat
     1981 agcgtatgat agcggcagaa tctgttgatg agagcttcat tctatgaaac ctgttttttt
     2041 ggattttgaa caacccatag ccgaactgac caacaaaatc gatgagctgc gtttcgtcca
     2101 agacgagtct gccgtcgata tttcggacga aatacaccgt ttgcagaaaa aaagcaacga
     2161 cctgaccaaa tcgattttca gcaaacttac gcccgcccag gtttcacagg tttcccggca
     2221 tccgcagcgt ccctatactt tggattacat tgatgcgctg tttactgatt ttgaagagct
     2281 gcacggcgac cgccactttg ccgacgatca tgcgattgtc ggcggattgg cgcgtttcaa
     2341 cggacaaagc gtgatggtcg tcgggcatca gaaagggcgc gacaccaaag aaaaaatccg
     2401 ccgcaacttc ggtatgccgc gccccgaggg ctaccgcaaa gccctgcgcc tgatgaaaac
     2461 ggctgagaaa ttcggcttgc ccgtcatgac ctttatcgat acgccgggcg cgtatcccgg
     2521 catcggcgcg gaagaacgcg ggcagtcgga agccatcggc aaaaacctgt acgaactgac
     2581 gcgcctgcac gtccccgttt tgtgtaccgt catcggcgaa gggggctcgg gcggtgcgtt
     2641 ggcggtcgcc gtgggcgatt acgtcaatat gctgcaatat tcgacctatt ctgttatctc
     2701 ccccgaaggc tgcgcttcga ttttgtggaa aaccgccgaa aaggcggcgg atgcggctca
     2761 ggctttgggc attactgccg accggctgca aaagctggac ttggtcgata ccgtcatcaa
     2821 agaaccattg ggcggcgcgc atcgggattt cgggcaaacc atgaaaaacg taaaagccgt
     2881 tttggaaaaa caactgcacg aagcgcaaag catcccgctt gccgatttgc tttcgcgccg
     2941 tttcgaccgc attatggctt acggcaaatt ttcggaacaa taattcaggt agaacaagca
     3001 gcaagcagtt tgtctgaaac tgcttgcttt ttctttatcg ggacggaacc gtgctgactt
     3061 tagatgcgtt tgagcaatgc ttgaaggatt gttttcctca aggtctgaat ggaaaaaaaa
     3121 cagcggtggc attaagcggc ggcttggatt ccgtcgtttt gctgcatctg cttgtccgcg
     3181 ccggaaaaaa gggcggtttt attccggatg cattgcatat ccatcacggc ttgagtcccc
     3241 gtgccgacga ttgggcagat ttctgccaaa
//
LOCUS       DAWHSK010000216         3267 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.216, whole genome shotgun sequence.
ACCESSION   DAWHSK010000216 DAWHSK010000000
VERSION     DAWHSK010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.216"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(381..1250)
                     /locus_tag="WIC03_001794"
     CDS             complement(381..1250)
                     /locus_tag="WIC03_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis lauroyl acyltransferase"
                     /protein_id="HEZ3884258.1"
                     /translation="MKFIFFVLYVLQFLPFALLHKLADLTGLLAYLLVKHRRRIGEIN
                     LAKCFPEWDGKKRETVLKQHFKHMAKLMLEYGLYWYAPAGRLKSLVRYRNKHYLDDAL
                     AAGGKVIILYPHFTAFEMAVYALNQDVPLISMYSHQKNKILDEQILKGRNRYHNVFLI
                     GRTEGLRALVKQFRKSSAPFLYLPDQDFGRNDSVFVDFFGIQTATITGLSRIAALANA
                     KVIPAIPVREADNTVTLHFYPAWKSFPSEDAQADAQRMNRFIEERVREHPEQYFWLHK
                     RFKTRPEGSPDFY"
     gene            complement(1301..1837)
                     /gene="ruvC"
                     /locus_tag="WIC03_001795"
     CDS             complement(1301..1837)
                     /gene="ruvC"
                     /locus_tag="WIC03_001795"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEZ3884259.1"
                     /translation="MSATVRILGIDPGSRVTGFGIIDVRGRDHFYVASGCIKTPPDAP
                     LADRIAVIVRHIGEVVAVYKPQQAAVEQVFVNVNPASTLMLGQARGAALAALVSHKLP
                     VSEYTALQVKQAVVGKGKAAKEQVQHMVVQMLGLSGTPQADAADGLAVALTHALRNHG
                     LASRLNPSGMQVKRGRFQ"
     gene            complement(1840..2079)
                     /locus_tag="WIC03_001796"
     CDS             complement(1840..2079)
                     /locus_tag="WIC03_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HEZ3884260.1"
                     /translation="MPHTLPDISQCIRQNLEQYFKDLNGTEPCGVYDMVLHQVEKPLL
                     VCVMEQCGGNQSKASVMLGLNRNTLRKKLIQHGLL"
     gene            complement(2109..3119)
                     /gene="dusB"
                     /locus_tag="WIC03_001797"
     CDS             complement(2109..3119)
                     /gene="dusB"
                     /locus_tag="WIC03_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEZ3884261.1"
                     /translation="MHIGGYFIDNPIALAPMAGITDKPFRRLCRDFGAGWAVCEMLTS
                     DPTLRNTRKTLHRSDFVDEGGIVAVQIAGSDPQQMADAARYNVSLGAQLIDINMGCPA
                     KKVCNVQAGSALMQNEPLVAAILEAVVRAAGVPVTLKTRLGWHEDHQNLPVIAKIAED
                     CGITALAVHGRTRTQMYKGEARYELIAETKCRLNIPVWVNGDITSPQKAAAVLKQTAA
                     DGIMIGRGAQGRPWFFRDLKHYAEHGVLPPALSLAECAAAILNHIRAIHAFYGDSAGV
                     RIARKHIGWYIGEMPDGEQARREINRLDSAAAQYDMLAGYLERLAGKTDRWACGYRTN
                     AF"
BASE COUNT          766 a          843 c          832 g          826 t
ORIGIN      
        1 aatatatgga tttttatttt ttatatttca ataaatataa atttaatttt gatttatatt
       61 taaatttaag cataaaatgt caaatattaa aggaaacatg aaaggcatat attaaatatt
      121 tatttataac gctatgtttt taaagaaaat taattttaat atattaacta gattgtctgc
      181 atatattcat gggtttgcgg tatttcttcc aaaacctgct tcgaatttcc cgaccaagtc
      241 ttaaaaatat tgtttttgag atacttaaat agcagcgatt atcaaatgaa atctgttcat
      301 ataatctgcc attttgcatt taaaaaaaca atcaggagtt tcgactcgaa atgcctgata
      361 tgttttgtaa ttttacgtag tcagtaaaaa tcggggctgc cttccggacg ggttttaaaa
      421 cgcttgtgca gccaaaaata ctgctcggga tgttcgcgca cgcgttcctc gataaaacgg
      481 ttcatgcgct gcgcgtcggc ttgcgcatct tcactcggaa aggatttcca agccgggtag
      541 aaatgcagtg taactgtatt gtctgcctcg cggacgggaa tggcgggtat cacttttgca
      601 tttgcaagcg cggcaatgcg gctcaatccg gtaatcgttg ccgtctgaat accgaaaaaa
      661 tccacaaaaa ccgaatcgtt gcgtccgaaa tcctgatcgg gcagatacag gaacggcgcg
      721 ctgcttttac ggaactgttt gactagagcg cgcagccctt cggtgcgccc gataaggaag
      781 acgttgtgat agcggttgcg gcctttcaaa atctgttcgt ccaatatctt gtttttttgg
      841 tgggaataca tactaattag cggaacatcc tgattaagcg cgtacaccgc catctcgaac
      901 gcggtaaagt gcgggtacag gatgatgact tttccccccg ccgccagcgc gtcgtccaaa
      961 taatgcttat tgcggtaacg caccagcgat ttcaaacgcc cggcgggcgc gtaccaatat
     1021 aaaccgtatt ccaacattag tttcgccata tgtttgaaat gctgcttcaa tacggtttcg
     1081 cgcttttttc cgtcccactc gggaaagcat tttgccaaat tgatttcgcc gatacggcgg
     1141 cggtgtttga ccaaaaggta ggcaagcaaa cccgtcagat cggcaagttt gtgcagcagc
     1201 gcaaacggca gaaactgcaa aacatacagt acaaaaaata taaatttcat ctcgatacac
     1261 attttctttt cagacggcaa aatacaaatg ccgtctgaaa ctattgaaac cttccgcgct
     1321 tgacctgcat ccccgacggg ttgagccggg acgcaagccc gtggttgcgt aaggcgtggg
     1381 tcagcgcgac ggcaagaccg tccgccgcat ccgcctgcgg cgttcccgaa agccccagca
     1441 tctgcaccac catatgctgc acctgttctt tcgccgcctt gcctttgccg actaccgcct
     1501 gtttgacctg caaggccgtg tattccgaaa cgggcagctt atggctgacc aatgccgcca
     1561 atgccgcgcc cctagcctga cccagcatca gcgtcgatgc cggattgacg ttgacgaaca
     1621 cctgttccac cgccgcctgc tgcggcttgt aaacggcaac gacttcgccg atgtgccgca
     1681 caatcacggc aatcctgtct gccagaggcg catcgggagg cgttttgatg cagccggagg
     1741 cgacgtaaaa atgatcgcgc cccctgacat cgatgatgcc gaaacccgtt acgcggctgc
     1801 ccgggtcgat acctaagata cggacggttg ccgacatatt cacagcaaac cgtgttgaat
     1861 cagtttttta cgcaaagtat tgcggttcag tcccaacatg acggaggctt tggactggtt
     1921 gccgccgcat tgctccatca cgcacaccag cagcggcttt tccacctgat gcaataccat
     1981 atcgtacacg ccgcaaggtt cggtaccgtt caggtctttg aaatattgtt ccaaattttg
     2041 tctgatacat tgggaaatat cgggaagggt atggggcatg attgcacttt caaaggataa
     2101 tcaagtgttc agaaggcatt tgtgcggtag ccgcacgccc aacggtcggt ttttccggca
     2161 agtctttcaa gataacctgc aagcatgtcg tattgcgccg ccgcactgtc caagcggttg
     2221 atttcacgcc gcgcctgttc gccgtcgggc atttcgccga tgtaccaacc tatgtgtttg
     2281 cgtgcgatgc gtacgcctgc actatcgccg taaaacgcgt gtatggcgcg gatgtggttc
     2341 aaaatagcgg cggcgcattc tgccaaactc aaggcaggcg gtaaaacacc gtgttcggcg
     2401 taatgtttca aatcgcggaa gaaccacggt ctgccttgcg cgccgcgccc tatcatgatg
     2461 ccgtcggcgg cggtttgttt gaggacggcg gcggcttttt gcggcgaagt gatgtcgccg
     2521 ttgacccaga ccgggatgtt cagacggcat ttggtttcgg cgatgagttc gtaacgcgcc
     2581 tcgcctttgt acatttgcgt gcgcgtgcgt ccgtggacgg caagggcggt gatgccgcaa
     2641 tcttcggcga ttttggcgat gacgggcagg ttttgatggt cttcgtgcca gcccaaacgg
     2701 gttttgaggg taacgggtac gcctgccgca cggacgacgg cttccaaaat ggcggcaacc
     2761 agcggctcgt tctgcatcag cgcgctgccg gcttggacgt tgcacacttt tttggcggga
     2821 cagcccatat tgatgtcgat aagctgcgcc ccgaggctga cgttgtaacg cgccgcatcc
     2881 gccatctgct gcggatcgct gccggcaatc tgcacggcaa cgatgccgcc ttcatcgaca
     2941 aaatcgctgc ggtgcaaggt ttttctagta tttctgagcg tcgggtcgct ggtcagcatt
     3001 tcgcacaccg cccaacctgc gccaaaatct cggcaaagtc ggcggaacgg tttgtcggta
     3061 atgcccgcca tcggcgcaag tgcgatgggg ttgtcgataa aatagccgcc gatgtgcata
     3121 atggatccgc gtttcaaaaa agtacgccat tgtacatttt ttaagcagga tttccaatct
     3181 ccggacgcgc ccgcgattgg gtcggacacc gttttatggc ataatccgca cacagattcc
     3241 ctgccccgcc actcacagga gggcagt
//
LOCUS       DAWHSK010000217         3228 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.217, whole genome shotgun sequence.
ACCESSION   DAWHSK010000217 DAWHSK010000000
VERSION     DAWHSK010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.217"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(126..509)
                     /locus_tag="WIC03_001798"
     CDS             complement(126..509)
                     /locus_tag="WIC03_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884262.1"
                     /translation="MEKIKNYIEFKKLFKNITEKFVRDLDITLIEAAEYSIIEFEIYL
                     SEYPEDSTFIFTAWAIYLLENNCLNERGKDNFFIAEMKKCYQQTNLDRIKSIYSSSDY
                     HIFAKDVNKIQQTFKLNLANIMQQE"
     gene            complement(514..1021)
                     /locus_tag="WIC03_001799"
                     /pseudo
     CDS             complement(514..1021)
                     /locus_tag="WIC03_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675736.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deaminase"
     gene            complement(1650..2045)
                     /locus_tag="WIC03_001800"
     CDS             complement(1650..2045)
                     /locus_tag="WIC03_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884263.1"
                     /translation="MNQFCLSEYEKAFLCRIIENNMIWKEYLIEQIENSIIVSRDNTG
                     CGCYIDFQVNLERTNLNKYENIPIFFEGDITAPDKSDAATFLLYTTESLNKAGRIIDF
                     LEVAIAGTNYDEVEVYSWLKMENSLRFGL"
     gene            complement(2057..2770)
                     /locus_tag="WIC03_001801"
                     /pseudo
     CDS             complement(2057..2770)
                     /locus_tag="WIC03_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236085.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VENN motif pre-toxin domain-containing protein"
     gene            complement(2866..3138)
                     /locus_tag="WIC03_001802"
     CDS             complement(2866..3138)
                     /locus_tag="WIC03_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884264.1"
                     /translation="MNKRMKMCPACQQGYLYHSKPKYLHDEIILCDECDAVWLKGMNI
                     FYGEYEKDFYSYVPFMESQGITSECIWEGDLFDHPYYEDENSNDMD"
BASE COUNT          949 a          656 c          518 g         1105 t
ORIGIN      
        1 agcacacacc ttacacatgg attttaggtt tcatgcaggc tacagcttgc tgctgcggta
       61 taaaagtatt tttagtgatt tactgttttt ggtatttttg cccgacgggg ttaaaaatac
      121 agttgctact cttgctgcat gatattggca agatttaatt taaatgtttg ttgaatcttg
      181 tttacatcct tagcaaaaat atgatagtca ctcgaagaat agatagattt tattctatcc
      241 aaattagttt gttgataaca ttttttcatt tccgcgatga aaaaattatc ttttcctctt
      301 tcattgagac agttattttc taacagatag attgcccatg ctgtaaaaat aaatgtacta
      361 tcttccggat attcagataa ataaatttcg aattcaatta tagaatattc agctgcttca
      421 attaatgtta tatctaaatc cctgacaaat ttttccgtta tatttttaaa taatttcttg
      481 aattctatat aattttttat tttttccatg attctatttt cctgtaaaaa tatttaattg
      541 aatatttgga tagcgatttc taaactctaa aataacattg ctgcaagatt gacaggcctt
      601 taattctgta aatagatcaa ttctacctga tacattacga ttatttccga gttgctgtgc
      661 tatagtttca agtaatttat attctccatc tatatttctt aatgttcctc taggaggaga
      721 ggcaatatta tgatatttat caacagatat aggtttaaaa atttttgttt caggtaacga
      781 aataaatcca tgttcccctt tttggaaaga actaaatgct tccatccttt gcggtaatcc
      841 tttaacatca atttttgcta tcgcaagatt ccctatttgt cgatactctt ccggcagttt
      901 catgcggact ctctggcttt cagcagttaa aagcaggagc actgtcattg ctcagataac
      961 gggtaacagg tgtaggcttg gccgatattg cagcctgtgc agatacagat cctgcaacca
     1021 tcaatgtgcc attaacaacc tgaactgtac catgcagata ttctccatta gagattgctt
     1081 tcgctccctg tccaatgtta tatccccctg ctgccacagt agctgctttg acactgaagc
     1141 caagcggtcc gtttaataag taaagtgcag gttgtgcggc aacagctgat ttgtaatcaa
     1201 aagaatcata acgactcaat gtttgatagg ctttcgataa agtgttatta atatctttgg
     1261 aaggaatagt gagattaccc attgactggg gcgttttcag tatcgaagct gcccaagctg
     1321 tgttacctcc agaggcttgg ttaataaaat atgttaccta tgtatattgt ttcttcaaaa
     1381 atcaaccgta ttgtccttgg gtgattttcg taccagcaca atcagtctgc cctagttgac
     1441 agatcgcgcc acactagcca gatacagcgt atcattggta aaactgataa tgcggcaata
     1501 taaagaaaaa cttcaatagt atgttaacga aatttgacgc ataaaaatac gctaaaaaat
     1561 tttcgcttga ctaattaaaa atgggattga gcataaaatt ttaataattt atattattga
     1621 aaatatctca gattttcagt cagtttattt tacaagccga accgtaggct attttccatc
     1681 tttaaccatg aatagacctc aacttcatca taatttgtgc ctgcaatagc aacttccaga
     1741 aagtcaataa ttctacctgc tttattcaaa gactctgtag tatatagcag gaaagtcgct
     1801 gcatctgact tatccggtgc agtgatgtct ccttcaaaaa aaatagggat attttcgtat
     1861 ttatttagat tagttctttc taaatttacc tgaaaatcaa tgtagcaccc acatcctgta
     1921 ttatctctag atactattat tgaattttct atttgctcta ttaaatattc cttccatatc
     1981 atattgtttt caataattct acataagaaa gccttctcat attcagataa acaaaactgg
     2041 ttcatatatt ctacccttac attttcaaaa tagttgcaat acgagatact ccattgttga
     2101 catatacgcg aacaattacc gttataccat tgaccgttac agtaactgga taatttcctg
     2161 tttttgtcag ttttaaagca tccactgcct tttgcgtcgc tacaacagca ttttccatgg
     2221 agcctgattt ttgaatcaat gtattaccga tgcttccttg atgcccctta aagatgtgat
     2281 gaactttatt tagccaatct acaccgtatc tttcagccaa tagtcgagaa ttactttcac
     2341 ctattttttc catcgaagct aaaatccttt gagcataggc agcatcagct gttgaaaaat
     2401 ttgcttcgcc caacaaaagc cgtaattcct gaatatctcc tcgtaaaatt gcatttctta
     2461 ctcccgcttc taatgtggtt tgtggctgaa agagggaatt attctctacc gccacagtag
     2521 ccgcattcgc cgctgtattc acatccccgc ctacaacggc agacgcagtg ccggcaacca
     2581 gttttgaata ggcagtaatc ttcgctttag ctttttcgat ttcggtcgca ctcatacgac
     2641 tgaaatcagt attcttaacc aaagcctcac caacaatctc accgactgca gcgcctatcg
     2701 caccatcctg acacttaccc ttattcgccg ccgctgccgc acagcccgct atggcatggg
     2761 caatcttgtg ggtggtatat cgctgccagc cctctctgtt gataatcttg cgtatgatta
     2821 cgccgtttga tggctaatcg tacctacgca ggcttaaaat ttccatcaat ccatatcatt
     2881 tgagttttca tcttcataat atggatgatc aaacaaatct ccttcccaaa tacattcact
     2941 cgttatacct tgggattcca tgaaaggaac ataagaataa aaatcttttt catattctcc
     3001 ataaaatata ttcatacctt tgagccatac tgcatcgcat tcatcacaca gaataatttc
     3061 atcatgaaga tatttaggtt tcgaatggta gagatagcct tgttgacaag caggacacat
     3121 tttcattctc ttattcatga ttactccata taatcgattt atttttgtgg gtaagcagta
     3181 gtgatttgat tgctgccttt agtctgtaca acaatacgta tatagcaa
//
LOCUS       DAWHSK010000218         3173 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.218, whole genome shotgun sequence.
ACCESSION   DAWHSK010000218 DAWHSK010000000
VERSION     DAWHSK010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.218"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            56..1009
                     /locus_tag="WIC03_001803"
     CDS             56..1009
                     /locus_tag="WIC03_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEZ3884265.1"
                     /translation="MTHTVHLHLEETDNLALQRLCGSFDNNLDLLAKALDIHISRRFE
                     HFTFNGAFAHAGKRALLKLLETAQTRDLNDGDIRLAAVEAQTEDAGHQEKNHDHAYYF
                     RTKRGSIGGRTPRQNGYIRALLNHDIVFGLGPAGTGKTYLAVAAAVDAMEKHQVERII
                     LVRPAVEAGEKLGFLPGDLTQKVDPYLRPLYDALYDLMGFDRVTKLIEKGLIEIAPLA
                     YMRGRTLNGAYIILDEAQNTTPEQMKMFLTRIGFGAKAVITGDTSQIDLPKNIKSGLK
                     DAREKLHGVEGLYFHTFTGEDVVRHPLVQKIVEAYESAEHD"
     gene            complement(1114..1437)
                     /locus_tag="WIC03_001804"
     CDS             complement(1114..1437)
                     /locus_tag="WIC03_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884266.1"
                     /translation="MKKCILGILTACAAMPAFADRIGDLEARLAQLEHRVAVLESGSN
                     TVKIDLFGSNSTMYVCSVTPFQKTFEASDRNEGVARQKVRQACNRETSAMFCEDEAIR
                     CRKFD"
     gene            complement(1610..2971)
                     /locus_tag="WIC03_001805"
     CDS             complement(1610..2971)
                     /locus_tag="WIC03_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HEZ3884267.1"
                     /translation="MLKKWLNKMLPSGRSSKKAESKTIIPAERHNIRAEMLSFAAENV
                     IRRLKGAGFQAYVVGGAVRDLLLGIEPKDFDVATDATPEQVHKLFRRSRIIGRRFQIV
                     HVMNGAEIIEVTTFRGGAKVHQNAHGRIMKDNTYGSIEEDAMRRDFTCNALYYDPEKE
                     EILDFHNGIADVAARRLVMIGDAAERYQEDPVRILRAIRLSSKLGFDLSEETAAPIAE
                     SICRLKHEPVARLFDEIMKLLFSGHARECLKRLNGFDIPDDIHPLLNALRVSDGIAGK
                     MTVLALKNTDERLRADKSVSVGFVLAALMWPELERHWKSNLQQGLKPVPALSDAINTM
                     RETVERGWGVPQRFSATMREIWMFQPQFENRKGARPHKLFAQARFRAAYDFLLLRAET
                     GNADRALAEWWTAFQTASTEQRSEMTKNEAAARHEKNEGQAKKRRRRRRKPKPKVVGT
                     DWE"
BASE COUNT          744 a          928 c          768 g          733 t
ORIGIN      
        1 aatctaatcc actatagatt aaaatcacac aatatccaac tattcggacc ttcatatgac
       61 acataccgtc cacctgcatt tagaagaaac cgacaacttg gcgttgcagc gtctgtgcgg
      121 ttcttttgac aacaaccttg atttacttgc taaagcactc gatatccaca tcagccgccg
      181 ttttgaacat ttcactttca acggcgcatt tgcacacgcc ggcaaacgcg cactgctcaa
      241 actcttggaa acggcgcaga cgcgcgacct aaacgacggc gacatcaggc ttgccgccgt
      301 cgaagcccaa accgaagatg ccggtcatca agaaaaaaac catgaccacg cctattattt
      361 ccgtaccaaa cgcggcagca tcggcggcag aacgccacgg cagaacggct atatccgcgc
      421 cctactcaac cacgacatcg tattcggtct cgggccggca ggtacgggca aaacctatct
      481 cgccgtcgcc gccgccgtcg atgcgatgga aaaacaccaa gtcgaacgca tcattttagt
      541 gcggcctgcc gtcgaagccg gcgagaaact gggttttctg cccggtgacc tgacccagaa
      601 agtcgatccc taccttcgtc cgctttatga tgccctctat gacctgatgg gctttgaccg
      661 tgtcaccaag ctgattgaaa aaggcctgat tgaaatcgcc ccgctcgcct atatgcgcgg
      721 caggacgctc aacggcgcat acatcatcct cgacgaagcg caaaacacca cgcccgaaca
      781 aatgaaaatg ttcctgaccc gcatcggctt cggcgcgaaa gccgtcatta ccggcgacac
      841 cagccaaatc gacctgccca aaaacatcaa atcgggatta aaagatgcgc gtgagaaact
      901 gcacggcgtg gaagggctgt atttccacac ctttaccggc gaagacgttg tccggcatcc
      961 tttggtgcaa aaaatcgtcg aagcctacga atcggcagaa cacgactgac attccaatgc
     1021 cgtctgaaac ccgatgcatg gaaaatcggg cagagaaaaa cagatacgga agccaagctc
     1081 cgtatccgtt tctttatccg tccaaccgat acatcaatcg aattttctgc atcggattgc
     1141 ctcatcttcg caaaacattg ccgaagtttc gcggttgcac gcctgacgca ctttctgccg
     1201 cgccacgcct tcattccgat cgcttgcctc aaacgtcttc tgaaaaggcg taacgctgca
     1261 tacatacatg gtggaatttg aaccgaaaag gtcgattttg acggtattgc tgccgctttc
     1321 caatacggcg acacggtgtt ccaactgcgc cagacgtgct tccaaatcgc cgattctgtc
     1381 ggcaaatgca ggcatggcgg cacacgcggt caaaatgccc aaaatacatt ttttcatcaa
     1441 tttttccttg ttgcttgaac cctgcccacc cggacatcct ttttccgaga ggcagggtta
     1501 gactttattt acggggatga atccccttac aaatcaggat aatacataga acggcacttt
     1561 atgcttcatc ccatgtgttg aaacttcatt gctccttgtc tgttgaccct tattcccaat
     1621 ccgttcccac aaccttcggc ttgggtttgc gcctgcgacg gcggcgtttt ttcgcctgtc
     1681 cttcgttttt ttcgtgtcgg gcggcggctt cgtttttggt catctccgac cgctgttccg
     1741 tcgatgccgt ctgaaacgcc gtccaccact cggcaagggc gcggtccgca ttgccggttt
     1801 cggcgcgcaa gagcaggaaa tcataggcgg cacggaaacg cgcctgtgca aacagtttgt
     1861 gcggacgtgc gcctttgcgg ttttcaaact gcggctggaa catccaaatt tcgcgcatcg
     1921 tggcggaaaa gcgttgcggt acgccccaac cgcgttcgac ggtttcgcgc atcgtattga
     1981 ttgcatcgga cagggcgggc acgggtttca aaccctgttg cagattgctt ttccaatggc
     2041 gttccaactc gggccacatc agggccgcca gtacgaaacc gaccgaaacc gatttgtcgg
     2101 cacgcagccg ctcatcggta tttttcaggg caagcaccgt catttttccg gcgatgccgt
     2161 ctgaaacgcg caaggcattg agcagcggat ggatgtcgtc cggtatgtca aatccgttca
     2221 aacgtttcag acactcgcgc gcgtgccctg aaaacagcag tttcataatt tcgtcgaaca
     2281 gccgcgctac cggttcgtgc ttcagacggc atatcgattc ggcaatcggt gcggcggttt
     2341 cttccgacag atcaaagccc aatttgctcg acaggcggat ggcgcgcaaa atcctgacag
     2401 ggtcttcctg atagcgttcg gcggcatcac caatcataac cagcctgcgg gcggcaacat
     2461 cggcaatccc gttgtggaaa tccaaaatct cttctttttc aggatcgtaa tacaaggcat
     2521 tgcaggtaaa atcgcgccgc atcgcatctt cttcgatgct gccgtaggtg ttgtccttca
     2581 tgattctgcc gtgcgcgttc tgatgaacct tcgcaccgcc gcgaaacgtc gttacttcga
     2641 taatctccgc accgttcatc acatggacaa tctgaaaacg cctgccgatg atgcggctgc
     2701 ggcggaagag tttgtgcacc tgttcgggcg tggcatcggt tgcgacatcg aaatccttgg
     2761 gttcgatgcc gagcagcagg tccctgaccg cgccgccgac cacataagcc tgaaaccccg
     2821 cccctttcag gcggcgtatg acgttttcgg cggcaaagct caacatttcg gcacggatgt
     2881 tgtgtctttc ggcaggaatg attgttttac tttccgcttt tttactgctc cgaccggaag
     2941 gcagcatctt attcaaccat tttttcagca taaatattct aacccgaaga gcggcatctc
     3001 cacgggcaac cgtgttcaga cggcatcagg cggtacgatt ccgcaagatg ccgtaaccat
     3061 aaacaaaagg cgtagaaact acgccgtccg aatcacgccg ccctcgcggc ggcggcaacg
     3121 cgccattata acagattgcc ctttaaaaaa atcagaccct gcttttgtgg cgg
//
LOCUS       DAWHSK010000219         3167 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.219, whole genome shotgun sequence.
ACCESSION   DAWHSK010000219 DAWHSK010000000
VERSION     DAWHSK010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.219"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..1343
                     /gene="argS"
                     /locus_tag="WIC03_001806"
     CDS             <1..1343
                     /gene="argS"
                     /locus_tag="WIC03_001806"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEZ3884268.1"
                     /translation="EMHVGHLRSSIIGDSISRVLAFMGNTVVRQNHVGDWGTQFGMLV
                     AYLVEQQKDNAAFELADLEQFYRAAKVRFDEDPAFADTAREYVVKLQGGDETVLALWK
                     QFVDISLSHAQAVYDTLGLKLRPEDVAGESKYNDDLQPVVDDLVQKGLAVEDDGAKVV
                     FLDEFKNKEGEPAAFIVQKQGGGFLYASTDLACLRYRVGTLHADRLLYVVDHRQALHF
                     EQLFTTSRKAGYLPENVGAAFVGFGTMMGKDGKPFKTRSGDTVKLVDLLTEAVERAAA
                     LVKEKNPELGADEAAKIGKTVGIGAVKYADLSKNRTSNYVFDWDAMLSFEGNTAPYLQ
                     YAYTRVQSVFRKAGEWDATAPTVLSEPLEKQLAAELLKFEDVLQSVADTAYPHYLAAY
                     LYQIATLFSRFYEACPILKAESASRNSRLQLAKLTGDTLKQGLDLLGIDVLDVM"
     gene            1877..>3167
                     /locus_tag="WIC03_001807"
     CDS             1877..>3167
                     /locus_tag="WIC03_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEZ3884269.1"
                     /translation="MNIHTLLSKQWTLPPFLPKRLLLSLLILLAPNAMFWVLALLTAT
                     ARPIVNLDYLPAALLIALPWRFVKIAGVLAFWLAVLFDGLMMVIQLFPFMDLIGAINL
                     VPFILTAPAPYQIMTGLLLLYMLAMPFVLQKAAAKTDFRHIAACAAVVAAAGYFTGHL
                     SYYDRGRMANIFGANNFYYAKSQAMLYTVSQNADFITAGLVDPVFLPLGNQQRAATHL
                     NEPKSQKILFIVAESWGLPANPELQNATFAKLLAQKDRFSVWESGSFPFIGATVEGEM
                     RELCAYGGLRGFALRRAPDEKFARCLPNRLKQEGYATFAMHGAGSSLYDRFSWYPRAG
                     FQEIKTAENLIGKKTCAIFGGVCDSELFGEVSAFFKKHDKGLFYWMTLTSHADYPESD
                     IFNHRLKCTEYGLPAETDLCRNFSLHTQFFDQLADLIQ"
BASE COUNT          713 a          976 c          805 g          673 t
ORIGIN      
        1 aggaaatgca tgtcggtcac ctgcgttcca gcatcatcgg cgacagcatt tcgcgcgtgt
       61 tggcatttat gggcaacacc gtcgtccgtc aaaaccacgt cggcgactgg ggtacgcagt
      121 tcggcatgtt ggtcgcttat ttggtcgagc agcaaaaaga caatgccgcg tttgagctgg
      181 cggatttgga gcagttttac cgcgccgcca aagtgcgctt tgacgaagat cctgccttcg
      241 ccgacaccgc gcgcgaatac gttgtgaagc tgcaaggcgg cgatgaaacc gttttggcat
      301 tgtggaaaca gtttgtcgat atttcgctct cgcacgccca agccgtttac gacacgctgg
      361 gcttgaagct gcgccccgaa gacgtggcgg gcgaatcgaa gtacaacgac gatttgcagc
      421 cagtggtcga tgatttggtt caaaaaggtc tggcggtcga agacgacggc gcgaaagtcg
      481 tattcttgga cgagttcaaa aacaaagaag gcgaacccgc cgcgtttatc gtgcaaaaac
      541 aaggcggcgg cttcctttat gcttccaccg acttggcgtg tttgcgctac cgtgtcggca
      601 cgcttcatgc cgaccgcctg ctgtacgtcg tcgaccaccg ccaagccctg cactttgagc
      661 aactctttac cacttcccgc aaagcaggct atctgccgga aaacgtcggc gcggcatttg
      721 tcggcttcgg cacgatgatg ggcaaagacg gcaaaccgtt caaaacacgc agcggcgaca
      781 ctgtaaaact ggtcgatctg ctgaccgaag ccgtcgaacg cgccgccgct ttggtgaaag
      841 agaaaaatcc cgaattgggc gcggacgaag ccgccaaaat cggcaaaacc gtcggcatcg
      901 gcgcggtcaa atacgccgac ttgagcaaaa accgcaccag caattatgtg ttcgactggg
      961 acgctatgct ttcgtttgaa ggcaacaccg ccccctacct gcaatatgcc tacacccgcg
     1021 tgcaaagcgt gttccgcaaa gccggtgaat gggacgcaac cgcaccgact gttttgagcg
     1081 aaccgctgga aaaacagctt gccgccgagc tgctgaaatt cgaagacgtg ctgcaaagcg
     1141 tggcggacac ggcgtatccg cactacctcg ccgcctacct ctatcaaatt gcgaccctgt
     1201 tcagccgctt ctacgaagcc tgtccgatac tcaaagccga aagcgcaagc cgcaacagcc
     1261 gcctgcaact ggcgaagctg accggcgaca cgctgaaaca aggcttggat ttgctgggca
     1321 tcgatgtgtt ggacgtaatg taaaaccgca ccgcccgatt gcggacaaca gcctcgccat
     1381 ccttatccga atctgaaaaa agcggcgcga tacaccgtat ccgccgcccc tcccaaaatg
     1441 cgaaacaaac aaacgccaag caagcaagca agcaagcaag caagcaaaaa attataaccc
     1501 ccttcctgcc gacgcacgca ctttccgcgc ggcgcattcc ccttttcccg cccctcaaat
     1561 ccgccttttc ttcaggcagg gtttcagccc gcctcttttc cctgttttcc tttccccgac
     1621 acgcgtgcgc gccccctgcc gcactgtgct gcactttcgc gcccggacgg catcgttccg
     1681 ccatccggtt ctctgtttta aatacccctg tttcagaaag aaatgcagat gtttcaacac
     1741 acaggacgac acataaagca ccgccctatg tgttgccctg atttggaagg ggttacgcct
     1801 cccaaataaa gtctgatcct gccgccccga aggacagatg tccgagtggc gaagtttcaa
     1861 ccgaaaagga aatacgatga atattcacac cctgctctcc aaacaatgga cgctgccgcc
     1921 attcctgccg aaacggctgc tgctgtccct gctgatactg ctggccccca atgcaatgtt
     1981 ttgggttttg gcactgctga ccgccaccgc ccgcccgatt gtcaatttgg actatcttcc
     2041 cgccgcgctg ctgatcgccc tgccttggcg tttcgtcaaa attgccggcg tattggcgtt
     2101 ttggctggcg gttttgtttg acgggctgat gatggtgatc cagctcttcc cttttatgga
     2161 cctcatcggc gccatcaacc tcgtcccctt catcctgacc gcccccgccc cttatcagat
     2221 aatgaccggg ctgttgctgc tgtatatgct ggcgatgccg tttgtgttgc agaaagccgc
     2281 cgccaaaacc gacttccgac acattgccgc ctgtgccgcc gttgtggcgg cagccggcta
     2341 tttcactggc catttgagtt actacgaccg ggggcggatg gccaatatct tcggcgcaaa
     2401 caacttctac tacgccaaaa gtcaggcgat gctctacacc gtcagccaga atgccgactt
     2461 tattaccgcc ggcctggtcg atcccgtctt cctccccttg ggcaatcaac agcgtgccgc
     2521 cacgcatctg aacgagccga aatctcaaaa aatcctcttt atcgtcgccg aatcttgggg
     2581 gctgccggcc aatcccgaac ttcaaaacgc cacttttgcc aaactgctgg cgcaaaaaga
     2641 ccgtttttcg gtttgggaaa gcggcagttt tccctttatc ggcgcgacgg tcgaaggcga
     2701 aatgcgcgaa ctgtgtgcct acggcggttt gcgcgggttc gcactgcgcc gcgcgcccga
     2761 cgaaaaattt gcccgctgcc tccccaaccg tttgaaacaa gaaggttacg ccacctttgc
     2821 gatgcacggc gcgggcagtt cgctttacga ccgcttcagc tggtatccga gggcgggctt
     2881 tcaagaaatc aaaaccgccg aaaacctgat cggtaaaaaa acctgcgcca ttttcggcgg
     2941 cgtgtgcgac agcgagctgt tcggcgaagt gtcggcattt ttcaaaaaac acgacaaggg
     3001 actgttttac tggatgacgc tgaccagcca cgccgactat cccgaatccg acattttcaa
     3061 ccacaggctc aaatgcaccg aatacggcct gcccgccgaa accgacctct gccgcaattt
     3121 cagcctgcac acccaattct tcgaccaact ggcggatttg atccaac
//
LOCUS       DAWHSK010000220         3165 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.220, whole genome shotgun sequence.
ACCESSION   DAWHSK010000220 DAWHSK010000000
VERSION     DAWHSK010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.220"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(198..1049)
                     /locus_tag="WIC03_001808"
     CDS             complement(198..1049)
                     /locus_tag="WIC03_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-54 factor"
                     /protein_id="HEZ3884270.1"
                     /translation="MTLLGIKLKQTQQLNQRLQQSLRVLQMSGIELEREVENWLSDNP
                     LLERKDTDEFSDAEFSHYTAPARQIGGDEGEDMLSNIAGEQDFKQYLHAQVCEHPLSD
                     QESACVHILIDFLDEQGYLTDSIEDILDHTPLEWMLDEAMLKQALTALKKFDPAGVAA
                     ADLNESLILQIERSGECAAKPSALHIVRNALDSIDGNRSQTLARIKKRLPQTDSGTLE
                     AALDLIASLNPFPAAGFASSTPTPYSDEALANLLAFRGMEVSRRTIAKYRESFEIPAA
                     HKRKTAE"
     gene            1397..2911
                     /gene="katA"
                     /locus_tag="WIC03_001809"
     CDS             1397..2911
                     /gene="katA"
                     /locus_tag="WIC03_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase KatA"
                     /protein_id="HEZ3884271.1"
                     /translation="MTTSKCPVTHLTMNNGAPVADNQNSLTAGPRGPLLAQDLWLNEK
                     LADFVREVIPERRMHAKGSGAFGTFTVTHDITKYTRAKIFSEVGKKTEMFARFTTVAG
                     ERGAADAERDIRGFALKFYTEEGNWDVVGNNTPVFFLRDPRKFPDLNKAVKRDPRTNM
                     RSATNNWDFWTLLPEALHQVTIVMSDRGIPAGYRHMHGFGSHTYSFWNEAGERFWVKF
                     HFRTQQGIKNLTNEEAAKIIADDRESHQRDLYEAIERGEFPKWTMYIQVMPEADAEKV
                     PYHPFDLTKVWPKKDYPLIEVGEFELNRNPENFFADVEQSAFAPSNLVPGVGASPDKM
                     LQARLFNYADAQRYRLGVNFRQIPVNRPRCPVHSNQRDGQGRADGNYGSLPHYEPNSF
                     GQWQQQPDFAEPPLKINGDAAHWDYRQDDDDYFSQPRALFNLMNDAQKQALFGNTAAA
                     MGDAPDFIKYRHIRNCYRCDPAYGEGVAKALGLTVEDAQAARATDPALGQAGLL"
     gene            2987..>3165
                     /locus_tag="WIC03_001810"
     CDS             2987..>3165
                     /locus_tag="WIC03_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884272.1"
                     /translation="MPSETSFDRFRRHFPIPPPPFQWAFFIKRKISRHSRPTAHFSAV
                     IPAPSPPVQTGTKTFP"
BASE COUNT          752 a          892 c          822 g          699 t
ORIGIN      
        1 cgtcattccc gcgaaagcgg gaatccaggt tcgttgagtt tcggtcattt ccgataaatt
       61 cttgcagctt tgcgtttcta gattccctct ttcgcgggaa tgacgggatg caggtttccg
      121 cgcggacgga ttcggcattc ccgctttcgc agaaatgccg gcttttggtt tgttgtcttt
      181 ataagattat tcggcaatta ttctgcggtt ttgcgtttgt gtgctgccgg aatctcaaag
      241 gattctctgt atttggcaat ggtgcggcga gaaacctcca tgccgcggaa agccagcagg
      301 ttggcgagcg cctcgtcaga atacggcgtg ggcgtggacg aggcaaaacc ggcggcggga
      361 aagggattga gcgaagcaat gaggtcgagt gcggcttcga gtgtgccgct gtcggtttgg
      421 ggcaggcgtt tttttattcg cgcgagggtt tggctgcggt tgccgtcaat gctgtcgagg
      481 gcgtttcgga cgatatgcag ggcggagggt ttggcagcac attcgcccga tctttctatc
      541 tgcagtatca gcgattcgtt caaatcggcg gcggccacgc ctgccgggtc gaattttttc
      601 aatgcggtca gggcttgttt cagcattgct tcatccaaca tccactccaa gggcgtatgg
      661 tcgaggatgt cttcgatgct gtcggtcaga taaccttgct cgtcaaggaa atcgatgagg
      721 atgtggacac aggcggattc ttggtcggaa agcgggtgtt cgcatacttg cgcgtgcagg
      781 tattgcttga aatcctgctc gccggcgatg ttggacagca tatcttcgcc ttcgtctccg
      841 ccgatttgac gggcaggcgc agtgtaatgg ctgaactcgg catcggaaaa ttcatccgtg
      901 tctttgcgtt cgagcagggg gttgtccgac agccagtttt cgacctcgcg ttcaagttcg
      961 atacccgaca tctgcaatac gcgcaaagat tgttgcagcc gctggttgag ctgctgggtc
     1021 tgtttgagct ttattccgag taaggtcatg ataatgtggg gaaaattgtt attttcagcc
     1081 tgtcggcgca aaaaatgccg caaagcgtca ttgcattata aatggtttta atgagcgggt
     1141 tcggattccg ttcgataaca aaaaacaaac gaaaatcaag aaccgattgc ttataataat
     1201 attaaatcga tttcatagtt ttaatagcga aaatcttggc gtatagtcgc atccatagtt
     1261 tttacaaaag ggaaataaaa tgtcgattca agaaatttat tgcaatcaag aaaccggtta
     1321 cgaatacgct ttccgccaaa tcgtactgta agccgtttac ccccatttga ccaacctgag
     1381 aaaaggaaca agagcgatga ctacctccaa atgccctgta acccatctga ccatgaacaa
     1441 cggcgcgcct gttgccgaca atcaaaacag cctgaccgcc ggtcctcgcg gccctctgct
     1501 ggcgcaggat ttgtggctga atgaaaaact cgccgacttc gtgcgcgaag tcatccccga
     1561 acgccgtatg cacgccaaag gttcgggcgc gttcggtacg tttaccgtaa cgcacgacat
     1621 caccaaatac acccgcgcca aaatcttcag cgaagtcggc aaaaaaaccg agatgttcgc
     1681 ccgtttcacc accgtggcag gcgaacgcgg cgcagccgat gcagaacgcg acatccgcgg
     1741 ttttgccttg aaattttata ccgaagaagg caactgggat gtggtcggca acaacacgcc
     1801 cgtgttcttc ctgcgcgacc cgcgtaagtt ccccgacctg aacaaagccg tcaaacgcga
     1861 cccgcgcacc aatatgcgct ctgccacaaa caactgggac ttctggacgc tgctgcccga
     1921 agcactgcac caagttacca tcgtgatgag cgaccgcggc atccccgccg gctaccgcca
     1981 tatgcacggc ttcggttcgc atacctacag cttctggaac gaagcaggcg agcgtttttg
     2041 ggtgaaattc catttccgca cccaacaagg cattaaaaac ctgaccaacg aagaagccgc
     2101 caaaatcatc gccgacgacc gcgaaagcca tcagcgcgac ttatacgaag ccatcgaacg
     2161 cggcgagttt ccgaaatgga cgatgtacat ccaagtcatg cctgaagcag acgcggaaaa
     2221 agtaccttat catccgtttg acttgaccaa agtttggccg aaaaaagact atccgctgat
     2281 tgaagtgggc gaattcgagt tgaaccgcaa tcccgaaaac ttcttcgccg atgtggaaca
     2341 atccgccttc gcaccgagca acctcgttcc cggtgtcggc gccagcccag ataaaatgtt
     2401 gcaagcccgt ttgttcaatt acgccgacgc gcaacgctac cgcttgggcg tgaacttccg
     2461 ccaaatccct gtcaaccgtc cgcgttgccc tgttcacagc aaccagcgcg acggacaagg
     2521 acgcgccgac ggcaactacg gcagcctgcc gcactacgaa cccaacagct tcggccaatg
     2581 gcagcagcag cccgacttcg ccgaaccgcc tttgaaaatc aacggcgacg cggcgcactg
     2641 ggactaccgc caagacgatg acgactattt cagccaaccg cgcgccctgt tcaacctgat
     2701 gaacgacgcg cagaaacagg cattgttcgg caacaccgcc gccgcaatgg gcgacgcgcc
     2761 cgacttcatc aaataccgcc atatccgcaa ctgctaccgt tgcgatccgg catacggcga
     2821 aggcgtggca aaagccctcg gactgactgt cgaagacgca caagccgccc gcgcgaccga
     2881 ccccgcactg ggtcaggccg gtttgctgta agggggcatt atgtggatgg aaattgaaga
     2941 aatcctgtcc cgcgccgtcc gctaccggaa ataaccgggc ataaaaatgc cgtctgaaac
     3001 gtcgtttgac cgcttcagac ggcatttccc catcccgccc ccgccgtttc agtgggcgtt
     3061 ttttattaaa cgcaaaatat cccgtcattc ccgccccaca gcccactttt ccgccgtcat
     3121 tcccgcacca tccccaccgg ttcaaaccgg cacgaaaact tttcc
//
LOCUS       DAWHSK010000221         3108 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.221, whole genome shotgun sequence.
ACCESSION   DAWHSK010000221 DAWHSK010000000
VERSION     DAWHSK010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.221"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(273..518)
                     /locus_tag="WIC03_001811"
     CDS             complement(273..518)
                     /locus_tag="WIC03_001811"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884273.1"
                     /translation="MPSELQTAFPASQLQRERTHTAIPAKNRKSKKKPKIRHSRAGGN
                     PIRPVSVFFEFQVTSKPPFPQKRESRNSKLQEFIKND"
     gene            complement(531..1115)
                     /gene="rnhB"
                     /locus_tag="WIC03_001812"
     CDS             complement(531..1115)
                     /gene="rnhB"
                     /locus_tag="WIC03_001812"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEZ3884274.1"
                     /translation="MHILTAGVDEAGRGPLVGSVFAAAVILPEIFDLPGLTDSKKLSE
                     KKRDALAEMIKNQAVAWHVAAASPEEIASLNILHATMLAMKRAVDGLAVRPERIFIDG
                     NRIPEHLNIPAEAVVKGDSKIIEISAASVLAKTARDAEMYALAQRHPQYGFDKHKGYG
                     TKQHLEALKQYGVLPEHRRDFAPVRNLLAQQTLF"
     gene            complement(1183..3078)
                     /gene="mnmG"
                     /locus_tag="WIC03_001813"
     CDS             complement(1183..3078)
                     /gene="mnmG"
                     /locus_tag="WIC03_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEZ3884275.1"
                     /translation="MTHMIYPKTYDVIVVGGGHAGTEAALAAARMGAQTLLLTHNIET
                     LGQMSCNPSIGGIGKGHLVRELDALGGAMALATDKSGIQFRRLNASKGAAVRATRAQA
                     DRILYKAAIREMLENQENLDLFQQAVEDVTLDGDRISGVITAMGVEFKARAVVLTAGT
                     FLSGKIHIGLENYEGGRAGDPAAKSLGGRLRELNLPQGRLKTGTPPRIDGRTIDFSQL
                     TEQPGDTPVPVMSVRGNAEMHPRQVSCWITHTNTQTHDIIRSGFDRSPMFTGKIEGVG
                     PRYCPSIEDKINRFADKDSHQIFLEPEGLTTHEYYPNGISTSLPFDIQIALVRSMKGL
                     ENAHILRPGYAIEYDYFDPRNLKASLETKTIAGLFFAGQINGTTGYEEAAAQGLLAGA
                     NAVQYVREQDPLLLRREQAYLGVLVDDLITKGVNEPYRMFTSRAEYRLQLREDNADMR
                     LTEDGYKIGLVGEAQWRMFNEKREAIEREIQRLKTTWYTPQKLAEDEQIRVFGQKLSR
                     EANLHDLLRRPNLDYAALMTLEGAMPSERLSAEVVEQVEIQVKYQGYIDRQNEEIDSR
                     RDIETLKLPEDIDYSKVKGLSAEVQQKLNQHKPETVGQASRISGVTPAAVALLMVHLK
                     RGFKDAK"
BASE COUNT          540 a          764 c          961 g          843 t
ORIGIN      
        1 ttcccgcttt tgcgggaatg acgcggaaaa gttgctgtga tttcggataa attttcgtca
       61 cgcttaattt ctgttttatc cgataaatgc ctgcaatcta aaatttcgtc attcccgcaa
      121 aaacaaaaaa tcaaaacaga agcctaaaat ttcgtcattc ccgcgaaggc gggaatctag
      181 gtctgtcggt acggaaactt atcgggtaaa acggttttgc cagccctgag actccggatt
      241 cctgttttcg taggaatccg gttttttgag cttcagtcat ttttgataaa ttcttgcagc
      301 tttgagtttc tagattcccg cttttgcggg aatggcggtt tggaagttac ctgaaattca
      361 aaaaaaacgg aaaccggacg gattggattc ccgcctgcgc gggaatgacg gattttaggt
      421 ttcttttttg attttctgtt tttcgcggga atggcggtat gggttctttc tctttgaagt
      481 tgcgatgctg gaaatgccgt ctgaagttca gacggcattt ttgtgccggt ttaaaacagc
      541 gtctgctgcg cgagcaggtt tctgacgggg gcgaagtcgc ggcgatgttc gggcagcacg
      601 ccgtattgtt tgagggcttc cagatgctgc ttcgtgccgt aacctttgtg tttgtcgaaa
      661 ccgtattggg gatggcgttg cgccagtgcg tacatttccg catcgcgtgc ggtctttgcc
      721 aaaacggatg cggcggagat ttcgatgatt ttgctgtcgc ctttgacgac ggcttcggca
      781 gggatgttca aatgttcggg aatgcggttg ccgtcgatga atattctttc gggacgcaca
      841 gccaaaccgt caacggcgcg tttcatcgcg agcatggtgg cgtgcaggat gttgaggctg
      901 gcgatttctt cgggcgaggc ggcggcaacg tgccacgcaa ccgcctgatt ttttatcatt
      961 tcggcaagcg cgtcgcgttt tttctcgctg agttttttgg agtcggtcag tccgggcagg
     1021 tcgaatattt ccggaaggat gacggcggcg gcaaacacgc tgccgactaa aggcccgcgc
     1081 cctgcctcgt ccacgccggc ggtcagtatg tgcatgatgt ttcctgtcgg gatggcgcga
     1141 tgccgtctga acggtgtttc agacggcatc gcgccgatgt gtttatttcg cgtctttaaa
     1201 cccgcgcttc aaatgcacca tcagcaatgc cactgccgca ggggttacgc cggaaatgcg
     1261 gcttgcctgc ccgacggttt cgggtttgtg ctggttgagc ttttgctgca cttctgccga
     1321 caaaccttta actttgctgt aatcgatatc ctcgggcagc tttaaggttt cgatgtcgcg
     1381 gcggctgtcg atttcttcgt tttggcggtc gatatagcct tggtatttga cttggatttc
     1441 gacttgttcg accacttcgg cggagaggcg ttcagacggc atcgcgcctt cgagcgtcat
     1501 cagcgcggcg tagtcgaggt tcgggcggcg caggaggtcg tgcaggttgg cttcgcggct
     1561 gagcttttgc ccgaatacgc ggatttgttc gtcttcggcg agtttttgcg gcgtgtacca
     1621 cgttgttttc aaacgttgga tttcgcgttc gatggcttcg cgtttttcgt tgaacatgcg
     1681 ccattgcgct tcacccacca agccgatttt gtagccgtct tcggtcaggc gcatatcggc
     1741 gttgtcttcc ctgagttgca ggcggtattc ggcacggctg gtgaacattc ggtagggttc
     1801 gttcacgcct ttggtgatga ggtcgtccac caatacgccg agataggctt gttcgcggcg
     1861 cagcaggagc gggtcttgtt cgcggacgta ttgcacggcg ttcgcgcctg ccagcagtcc
     1921 ttgcgcggcg gcttcttcat aacctgtcgt accgttgatt tgcccggcga aaaacaatcc
     1981 ggcaatggtt ttggtttcga ggcttgcttt gaggttgcgc ggatcgaagt agtcgtattc
     2041 gatggcgtag ccggggcgca ggatatgggc gttttccaaa cctttcatac tgcggacgag
     2101 cgcgatttgg atgtcgaacg gcaggctggt ggagataccg ttggggtagt attcgtgcgt
     2161 agtcaggcct tcgggttcta ggaaaatctg gtggctgtct ttgtcggcga agcggttgat
     2221 tttgtcttcg atagacggac aataacgcgg gcccacgcct tcgattttgc cggtaaacat
     2281 cgggctgcgg tcgaagcctg agcggatgat gtcgtgggtt tgcgtgttgg tatgcgtaat
     2341 ccagcaggac acttggcgcg ggtgcatttc ggcgttgccg cgtacggaca tgacgggaac
     2401 gggcgtgtcg ccgggctgtt cggtcagttg ggagaagtca atcgtgcgtc cgtcgatgcg
     2461 cggcggcgtg ccggttttca gacggccttg cggcaggttc aattcgcgca aacgtccgcc
     2521 cagcgatttg gcggcggggt cgccggcgcg tccgccctcg tagttttcca aaccgatgtg
     2581 gattttgccg gacaaaaacg tgcctgcggt caacaccacg gcgcgtgctt taaactccac
     2641 gcccatcgcg gtgattacgc ctgaaatgcg gtcgccatcg agcgttacgt cttcgacggc
     2701 ttgttggaaa aggtcgaggt tttcttggtt ttccaacatt tcgcggatgg cggctttata
     2761 taggatacga tccgcctgcg cgcgcgtggc ccgtactgcc gcgcctttgc tggcgttcag
     2821 acggcggaac tggatgccgg atttgtcggt tgccaacgcc atcgcgccgc cgagcgcgtc
     2881 gagttcgcgc accaaatgcc ctttgccgat gccgccgatg gaagggttgc acgacatttg
     2941 tccgagcgtt tcaatattgt gtgtgagcaa aagcgtctgc gcgcccatac gggcggcggc
     3001 gagcgcggct tctgtgccgg cgtgtccgcc gccgacgacg ataacgtcgt aggttttggg
     3061 gtaaatcatg tgggtcatgt gtgtattgcc tgacggtgtt tcagacgg
//
LOCUS       DAWHSK010000222         3102 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.222, whole genome shotgun sequence.
ACCESSION   DAWHSK010000222 DAWHSK010000000
VERSION     DAWHSK010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.222"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..212)
                     /locus_tag="WIC03_001814"
     CDS             complement(<1..212)
                     /locus_tag="WIC03_001814"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884276.1"
                     /translation="MLPTRKTARKIQARHIALPFSGKPQFYRPQERLSKQAQATPPGH
                     FRFHRLSVVRIMQQHHQRITLFGRQQA"
     gene            272..895
                     /locus_tag="WIC03_001815"
     CDS             272..895
                     /locus_tag="WIC03_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEZ3884277.1"
                     /translation="MKKPTDTLPVNLQRRRLLCAAGALLLSPLAHAGAQREETLADDV
                     ASVMRSSVGSVNPPRLVFDNPKEGERWLSAMSARLARFVPEEEERRRLLVNIQYESSR
                     AGLDTQIVLGLIEVESAFRQYAISGVGARGLMQVMPFWKNYIGKPAHNLFDIRTNLRY
                     GCTILRHYRNLEKGNIVRALARFNGSLGSNKYPNAVLGAWRNRWQWR"
     gene            1006..2388
                     /locus_tag="WIC03_001816"
     CDS             1006..2388
                     /locus_tag="WIC03_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ3884278.1"
                     /translation="MARDNRIQMFPHEWRASTTLSGVYALRMLGMFLVLPVLAVYAAS
                     LPGAEGNKTLVGLAMGIYGLTQALLQLPLGIASDKFGRKKTIYAGLVVFAAGSFLAAA
                     ADTLPMLVAARAIQGAGAVSAAVTALLADLTRDGVRTRAMAMIGLSIGLTFSVSLVVA
                     PVIADAVGVRGLFMLTGILTVISIGVVAWMTPDPEVSKLHEDTQAQPSRIGEVLKNRR
                     LLTLDFGIFALHAAQMALFTALPFAMTQLGLEKIQHWKVYLPSTITGLVVMVPLIIVG
                     ETRNKLKQVFILGIACIAAAQLGLLSGMRSVGLITAYLVVYFIGFNVLEASLPSMVSK
                     IAPSDLKGTAMGVYNTMQSLGLFAGGAAGGLLFQKYGFSGVFAFCSILMLLWLVIAVL
                     SPAPKPVKNLSYPVGGVWQGNQEGLYRALSELEGVEDIGFSFDGQTVYLKVLQKGFDQ
                     AAAEKIITGV"
     gene            2392..2916
                     /locus_tag="WIC03_001817"
     CDS             2392..2916
                     /locus_tag="WIC03_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEZ3884279.1"
                     /translation="MSLNKVILIGRLGRDPEVRYMPNGEAVCNFSVATSETWNDRNGQ
                     RVERTEWHNITMYRKLAEIAGQYLKKGGLVYLEGRIQSRKYQGKDGIERTAYDIVANE
                     MKMLGGRNENSGGAPYEEGYGQSQEAYQRPAQQSRQPASDAPSHPQEAPAAPRRQPVP
                     AAAPVEDIDDDIPF"
BASE COUNT          625 a          843 c          936 g          698 t
ORIGIN      
        1 gcctgctgcc gcccgaaaag cgtgatgcgc tgatggtgct gctgcataat ccggacgacg
       61 gataaccggt gaaaacggaa atgcccgggc ggtgttgcct gtgcctgttt ggacaagcgt
      121 tcttgagggc ggtagaattg aggtttgccc gaaaagggca gggcgatatg ccgcgcttgg
      181 attttgcgcg cggtttttcg cgttggaagc aatcattgtc tgtttgaata tttaatgccg
      241 tctgaaacgt ccggacgcgc ccccgttccc tatgaaaaaa ccgaccgata ccctacccgt
      301 taatctgcaa cgccgccgcc tgttgtgtgc cgccggtgcg ttgttgctca gtcctctggc
      361 gcacgccggc gcgcaacgtg aggaaacgct tgccgacgat gtggcttccg tgatgaggag
      421 ttctgtcggc agcgtcaatc cgccgaggct ggtgtttgac aatccgaaag agggcgaacg
      481 ttggttgtct gccatgtcgg cacgtttggc aaggttcgtc cccgaggagg aggagcggcg
      541 caggctgctg gtcaatattc agtatgaaag cagccgggcc ggtttggata cgcagattgt
      601 gttggggctg attgaggtgg aaagcgcgtt ccgccagtat gcaatcagcg gtgtcggcgc
      661 gcgcggcctg atgcaggtta tgccgttttg gaaaaactac atcggcaaac cggcgcacaa
      721 cctgttcgac atccgcacca acctgcgtta cggctgtacc atcctgcgcc attaccggaa
      781 tcttgaaaaa ggcaacatcg tccgcgcgct tgcccgcttt aatggcagct tgggcagcaa
      841 taaatatccg aacgccgttt tgggcgcgtg gcgcaaccgc tggcagtggc gttaattttg
      901 aacccgcgcc gcaaccgaaa tacggcgaat cctgtataat ccgaaaatct gttcactgga
      961 agttcagacg gcattgcaac tgttgatgcc gtctgaaaaa atatgatggc aagagacaac
     1021 cgcatccaaa tgtttccgca cgaatggcgc gccagtacga cgctttccgg cgtgtacgcg
     1081 ctgcgtatgc tgggtatgtt cctcgtgctg cccgtattgg cggtgtatgc cgcctcgctg
     1141 cccggcgcgg aaggcaacaa aacgctggtc gggctggcaa tgggcattta cgggctgaca
     1201 caggctctgc tgcaactgcc tttgggcatc gcttccgaca agttcgggcg caagaaaacc
     1261 atttatgcgg gactggtcgt gtttgcggcg ggcagctttc ttgccgccgc cgccgatacg
     1321 ctgcccatgc tggtcgccgc acgcgccata cagggtgcag gggcggtcag tgcggcggtt
     1381 accgcgctgc tggcggattt gacgcgcgac ggcgtacgta cccgcgcaat ggcgatgatc
     1441 ggtttgagta tcggtttgac gttttcggtc agcctcgtcg ttgcccccgt gattgccgac
     1501 gcggtcggcg ttcgcggact gtttatgctg accggcattc tgaccgtcat cagcatcggc
     1561 gtggtggcgt ggatgactcc cgatcccgaa gtttccaagc tgcacgaaga tacgcaggcg
     1621 cagccttcgc gcataggcga agttttgaaa aaccgtaggc tgctgacgct cgatttcggc
     1681 attttcgccc tgcacgccgc gcaaatggca ttgtttaccg cgctgccttt tgcgatgacg
     1741 cagctcggtt tggaaaaaat ccagcattgg aaagtctatc tgccctcgac cattacgggc
     1801 ttggtggtga tggttccgct gattatcgtc ggcgagacgc gcaacaagct caaacaggtt
     1861 tttatcttgg gcatcgcctg catcgcggcg gcacagctcg gtttgctgtc cggtatgcgc
     1921 tcggtaggct tgattaccgc ttatttggtt gtttacttta tcggttttaa tgtgttggaa
     1981 gcgagcctgc cgtctatggt ttccaaaatc gcgccgtccg acctgaaggg tacggcgatg
     2041 ggcgtgtaca acacgatgca gtcgctcgga ctgtttgccg gcggcgcggc aggcggtttg
     2101 ctgtttcaaa aatacggctt ttccggcgtg tttgcctttt gcagtatatt gatgctgctg
     2161 tggctggtaa ttgccgtttt atcgcctgcg cccaagcccg tcaaaaacct cagttaccct
     2221 gtcggcggcg tgtggcaggg caatcaggaa gggttatacc gcgccttgtc ggagcttgag
     2281 ggtgtggaag acatcggttt cagtttcgac gggcagaccg tctatctcaa agtgttgcag
     2341 aagggtttcg atcaggctgc cgctgaaaaa atcatcacag gagtttaaaa aatgtcattg
     2401 aacaaagtca tcctcatcgg ccgcctcgga cgcgatcccg aagtgcgcta tatgcccaac
     2461 ggcgaggcgg tttgtaattt cagcgtcgcc accagcgaaa cttggaacga ccgcaacggc
     2521 caacgtgtag agcgtactga gtggcacaac atcaccatgt accgcaaact ggcggaaatt
     2581 gccgggcaat acctcaaaaa aggcgggctg gtttatttgg aaggcagaat ccaaagccgc
     2641 aaataccaag gcaaagacgg catcgaacgc accgcttacg atattgtcgc caacgaaatg
     2701 aaaatgttgg gcgggcgcaa tgaaaacagc ggcggtgcgc cttacgagga aggttacggt
     2761 cagagtcagg aggcttacca acgccccgcg cagcaaagcc ggcagcccgc ctccgacgcg
     2821 ccgtcccatc cccaagaagc accagccgcg ccgcgccgcc aacccgtgcc tgccgccgcc
     2881 ccggtcgagg acattgacga cgatatcccg ttctgaattt tacggccgga catcctcgcg
     2941 gagggaaatc ataaaggacg gagaaacctt aaaccttacg gggcagggtt tctctttttt
     3001 gcatttgggc cggcttgttg ccgccctttg cgttgggcgg aacgttgcga ttgcgccgtt
     3061 cgggcgtttt gaggcagtga tgcctgccgc acgtccccgc tg
//
LOCUS       DAWHSK010000223         3093 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.223, whole genome shotgun sequence.
ACCESSION   DAWHSK010000223 DAWHSK010000000
VERSION     DAWHSK010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.223"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..808
                     /locus_tag="WIC03_001818"
     CDS             <1..808
                     /locus_tag="WIC03_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ3884280.1"
                     /translation="QAQNSGLIPSLNIGLDELAKSGGGEYIARTDADDIAAPDWIEKI
                     VGEMEKDRSIIAMGAWLEVLSEEKDGNRLARHHRHGKIWKKPTRHEDIADFFPFGNPI
                     HNNTMIMRRSVIDGGLRYNTERDWAEDYQFWYDVSKLGRLAYYPEALVKYRLHANQVS
                     SKYSVRQHEIAQGIQKTARNDFLQSMGFKTRFDSLEYRQIKAVAYELLEKHLPEEDFE
                     RARRFLYQCFKRTDTLPAGAWLDFAADGRMRRLFTLRQYFGILHRLLKNR"
     gene            850..1677
                     /locus_tag="WIC03_001819"
     CDS             850..1677
                     /locus_tag="WIC03_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HEZ3884281.1"
                     /translation="MQNHVISLASAAERRAHIAATFGARGIPFQFFDALMPSERLEQA
                     MAELVPGLSAHPYLSGVEKACFMSHAVLWKQALDEGLPYIAVFEDDVLLGEGAEKFLA
                     EDAWLKERFDPDSAFIVRLETMFMHVLTSPSGVADYCGRAFPLLESEHWGTAGYIISK
                     KAIRFFLQHFVMLQPEQIKPIDLMMFSDFFDKEGMPVYQVSPALCAQELHYAKFHDQN
                     SALGSLIEHDRLLNRKQQRRDSPANTFKRRLIRALTKISREREKRRQRREQFIVPFQ"
     gene            1689..1784
                     /locus_tag="WIC03_001820"
     CDS             1689..1784
                     /locus_tag="WIC03_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lacto-N-neotetraose biosynthesis glycosyl
                     transferase"
                     /protein_id="HEZ3884282.1"
                     /translation="MDIVFAADGRMRRLFTLRQYFGILRRLLKNR"
     gene            1826..2668
                     /locus_tag="WIC03_001821"
     CDS             1826..2668
                     /locus_tag="WIC03_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HEZ3884283.1"
                     /translation="MQNHVISLASAAERRAHIADTFGRHDIPFQFFDALMPSEELNRV
                     MAELVPGLAKQHLLSEVEKACFMSHAVLWKQALDEGLPYVAVFEDDVLLGKDAEKFLA
                     EDTWLEERFDKDSAFIVRLETMFAKVIVRPDKVLNYENRSFPLLESEHWGTAGYIISR
                     EAMRFFLERFAVLPAEWIKAVDWMMFTYFFDKEGMPVYQVNPALCTQELHYAKFLSKN
                     SMLGSDLEKDREQERRHRRSLKVMFDLKRALGKFGREKKKRMERQRQAELEKAYGRRV
                     ISFK"
BASE COUNT          829 a          729 c          810 g          725 t
ORIGIN      
        1 acaagctcaa aattccggcc tgattccctc tttaaacatc gggctggacg aattggcaaa
       61 gtcggggggg ggggaatata ttgcacgcac cgatgccgac gatattgccg cccccgactg
      121 gattgagaaa atcgtgggcg agatggaaaa agaccgcagc atcatcgcga tgggcgcgtg
      181 gctggaagtg ttgtcggaag aaaaggacgg caaccggctg gcgcggcatc acaggcacgg
      241 caaaatttgg aaaaagccga cccggcacga agatattgcc gactttttcc ctttcggcaa
      301 ccccatacac aacaacacga tgattatgcg gcgcagcgtc attgacggcg gtttgcgtta
      361 caacaccgag cgggattggg cggaagatta ccaattttgg tacgatgtca gtaaattggg
      421 caggctggct tattatcccg aagccttggt caaataccgc cttcacgcca atcaggtttc
      481 atccaaatac agcgtccgcc aacacgaaat cgcgcagggc atccaaaaaa ccgccagaaa
      541 cgattttttg cagtctatgg gttttaaaac ccggttcgac agccttgaat accgccaaat
      601 aaaagcagta gcgtatgaat tgctggagaa acatttgccg gaagaagatt ttgaacgcgc
      661 ccgccggttt ttgtaccaat gcttcaaacg gacggacacg ctgcccgccg gcgcgtggct
      721 ggattttgcg gcagacggca ggatgcggcg gctgtttacc ttgaggcaat acttcggcat
      781 tttgcaccga ttgctgaaaa accgttgaaa aacgccgctt tatccaacag acaaaaaaca
      841 ggataaatta tgcaaaacca cgttatcagc ttagcttccg ccgcagaacg cagggcgcac
      901 attgccgcaa ccttcggcgc gcgcggcatc ccgtttcagt ttttcgacgc actgatgccg
      961 tctgaaaggc tggaacaggc aatggcggaa ctcgtccccg gcttgtcggc gcacccctat
     1021 ttgagcggag tggaaaaagc ctgctttatg agccacgccg tattgtggaa acaggcattg
     1081 gacgaaggtc tgccgtatat cgccgtattt gaggacgacg ttttactcgg cgaaggcgcg
     1141 gagaaattcc ttgccgaaga cgcttggcta aaagaacgct ttgaccccga ttccgccttt
     1201 atcgtccgct tggaaacgat gtttatgcac gtcctgacct cgccctccgg tgtggcggac
     1261 tactgcgggc gtgcctttcc gctgttggaa agcgaacact gggggacggc gggctatatc
     1321 atttccaaaa aagcgatacg gtttttcctg caacatttcg taatgttgca gccggaacag
     1381 ataaaaccta tcgacttaat gatgttcagt gattttttcg ataaggaggg gatgcctgtt
     1441 tatcaggtca gtccggcctt atgcgcccaa gagctgcatt atgccaagtt tcacgaccaa
     1501 aacagcgcat tgggcagcct gatcgaacac gaccgcctcc tgaaccgcaa acagcaaagg
     1561 cgcgattccc ccgccaacac attcaaacgc cgcctgatcc gcgccttgac caaaatcagc
     1621 agggaaaggg aaaaacgccg gcaaaggcgc gaacagttca ttgtgccttt ccaataaaag
     1681 gaggagagat ggacatcgta tttgcagcag acggcaggat gcggcggctg tttaccttga
     1741 ggcaatactt cggcattttg cgccgattgc tgaaaaaccg ttgaaaaacg ccgctttatc
     1801 caacagacaa aaaacaggat aaattatgca aaaccacgtt atcagcttag cttccgccgc
     1861 agaacgcagg gcgcacattg ccgatacctt cggcaggcac gacattccgt ttcagttttt
     1921 cgacgcactg atgccgtctg aagaactgaa tcgggtgatg gcggaattgg tgccgggatt
     1981 ggcaaaacag cacctgttga gcgaagtgga aaaagcctgc tttatgagcc acgccgtatt
     2041 gtggaaacag gcattggatg aaggtctgcc ctatgttgcc gtatttgagg atgatgtcct
     2101 gcttggcaaa gacgcagaaa agttccttgc cgaagatact tggttggaag agcgttttga
     2161 taaggattct gcctttatcg ttcgtttgga aacgatgttt gcgaaagtta ttgtcagacc
     2221 ggataaagtc ctgaattatg agaaccggtc atttcctttg ctggagagcg aacattgggg
     2281 gacggctggc tatatcattt cgcgtgaggc gatgcggttt ttcttggaaa ggtttgccgt
     2341 tttgccggca gagtggatta aagcggtaga ttggatgatg tttacttatt tctttgataa
     2401 ggaggggatg cctgtttatc aggttaatcc cgccttatgt acccaagaat tgcattatgc
     2461 caagttcctc agtaaaaaca gtatgttggg tagcgatttg gaaaaagata gggaacaaga
     2521 aagaagacac cgccgttcgt tgaaggtgat gtttgacttg aagcgtgctt tgggtaaatt
     2581 cggtagggaa aagaagaaaa gaatggagcg tcaaaggcag gcggagcttg agaaagctta
     2641 cggcaggcgg gtcatatcgt tcaaatagtt tgtgtaaaat atagcggatt aaaattagaa
     2701 atggatacac tgtcattccc gcgaaagtgg gaatccagtc cgttgagttt cagtcatttc
     2761 cgataaatca ccgcaacgtt aaatttctag attcccgctt ttgcgggaat gacggcgggg
     2821 gatttctgtt tttccgacaa attcctaaaa cttaaaattc cgttattccc gcgaaaacag
     2881 aaaaccaaaa atagaaacct aaaatctcgt cattcccgca aaaacagaaa accaaaaaac
     2941 agcaacctaa aattcgtcat tcccgcgcag gcgggaatcc agtccgttcg gtttcgcttg
     3001 ttttaagttt cgggcaactt ccacttcgtc attcccgcgc aggcgggaat ccagtccgtt
     3061 gaggttcagt catttccgat aaatcaccgc aac
//
LOCUS       DAWHSK010000224         3080 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.224, whole genome shotgun sequence.
ACCESSION   DAWHSK010000224 DAWHSK010000000
VERSION     DAWHSK010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3080
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.224"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(97..1116)
                     /gene="lepB"
                     /locus_tag="WIC03_001822"
     CDS             complement(97..1116)
                     /gene="lepB"
                     /locus_tag="WIC03_001822"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEZ3884284.1"
                     /translation="MNTMLMSGAAAALLAGIILYFKSDKKRQENGEWSSGLEYAYILT
                     AVGVFAALSLFMSFTAVFLIFVVLCGTAWGVYKYRLKTHPEISESSHFGDYFGSFFPT
                     VLVLFLIRSFIAEPFQIPSSSMRPGLIKGDFILVGKFSYGLRVPVLNNVFIPTGKIER
                     GDVVVFNYPLQPEMTYIKRIVGIPGDVVEYRDKILTVNGKPTSDIPNGTYRYPDDTDP
                     SEIHNTDMFRSGLDGKSFNILKKEGQPAVSLPVLGKYTSDIMSENGYSIEQSGLEHCQ
                     YADDSSGFVCKVPEGRYFAMGDNRDNSADSRYWGFVDDKLVVGKAMFILMNFGDFGRA
                     GTAIR"
     gene            complement(1259..3052)
                     /gene="lepA"
                     /locus_tag="WIC03_001823"
     CDS             complement(1259..3052)
                     /gene="lepA"
                     /locus_tag="WIC03_001823"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEZ3884285.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLADRFIQYCGGLDLREMSTQVLDSM
                     DIEKERGITIKAQTAALNYKARDGQVYQLNLIDTPGHVDFSYEVSRSLSACEGALLVV
                     DASQGVEAQTVANCYTAIDLGVEVVPVLNKIDLPAADPERVEQEIEDIIGIDAVGAVQ
                     CSAKSGIGVEDVLEEIVAKIPAPTGDENAPLQAVIVDSWFDNYVGVVMLIRVKNGTIK
                     LKDKVRFMSTKAETQVEQLGVFTPKSVQKQELKAGEVGFLITGVKELGQAKVGDTVTL
                     VANPATEPLPGFQEVQSQVFAGLYPVESHDYEALRDALEKLQLNDASLKFEPEVSQAL
                     GFGFRCGFLGLLHLEIVQERLEREFDMDLITTAPTVVYEVVLKNGEKIEVENPSKLPD
                     IGSIETILEPIITATILVPQEYVGNVMTLCNQKRGVQVNMQYMGRQVMLTYDLPMNEV
                     VMDFFDKLKSTSRGYASLDYHFKEFQPSDLIKLDIMVNGEKVDALSLIVHRQSAVHRG
                     RELASKMRELIPRQMFDIAVQAAIGSQIIARENVKALRKNVLAKCYGGDITRKKKLLE
                     KQKAGKRRMKQVGNVEIPQSAFLAILQVSDK"
BASE COUNT          716 a          797 c          793 g          774 t
ORIGIN      
        1 gtaggaaaat ccgacaacgt tagatccgcc tgcaaaagtg aggaatagca aatgccgtct
       61 gaaactattt tcagacggca ttcttggctt cctggcctaa cggattgccg taccggccct
      121 gccgaaatcg ccgaagttca tcaaaatgaa cattgccttg ccgacaacca gcttgtcatc
      181 cacaaatccc cagtagcgcg aatcggcact gttgtcgcgg ttgtcgccca tagcgaaata
      241 gcgtccttcg ggaactttgc acacgaaacc gctgctgtcg tcggcatatt ggcagtgttc
      301 caaaccgctt tgctctatgg aatatccgtt ttcagacata atatcggagg tatatttgcc
      361 caatacaggc agggaaacgg caggctgtcc ttcttttttc agaatattga aggatttgcc
      421 gtctagaccg ctgcggaaca tatccgtgtt gtggatttcg gaagggtcgg tgtcgtcggg
      481 ataacggtat gtgccgttag gaatatcgga agtgggtttg ccatttaccg tcaaaatctt
      541 atcccgatat tcgaccacat cgcccggaat gccgacaata cgcttgatgt aggtcatctc
      601 cggttgcaaa ggataattaa aaacgaccac atcgccccgt tcgattttgc ccgtggggat
      661 gaatacattg tttaaaacag gtacgcgcag gccgtaggaa aatttgccga ccaaaatgaa
      721 atcgcccttg atcaggcccg ggcgcattga gctggacggg atttggaacg gttcggcgat
      781 aaacgaccgg atgaggaaca ataccaaaac ggtagggaag aaactgccga aataatcgcc
      841 gaagtggctg ctttccgaga tttcgggatg agtcttcagg cggtatttat atacccccca
      901 agccgtaccg cacaatacaa cgaaaatcag gaaaacggcg gtaaagctca taaacaggga
      961 caaagcggca aacacgccga ccgctgtcag gatataggcg tattcgaggc cggaactcca
     1021 ttcaccgttt tcctgccgct ttttgtcgct tttgaaataa aggatgatgc cggcaagcag
     1081 cgcggcagcc gcgcccgaca ttagcatagt gttcattgtt attccttaat gcttaaaaaa
     1141 ccgcccgtcc gtgcaaccgt tttaaggcgg caaattgcaa aatttgtttg cgggcgcgtg
     1201 cccctgaaat cagggcggtt tgaggggtgt tcccgacgcg ccgccctgtg tgccagagtt
     1261 atttgtcgct cacctgcaaa atcgccaaga acgcgctttg cggaatttcc acattgccca
     1321 cttgtttcat acggcgttta cctgcctttt gtttttcaag cagttttttc ttacgcgtaa
     1381 tatcgccgcc gtaacatttc gccaagacgt ttttacgcag tgctttgacg ttttcgcggg
     1441 cgataatctg gctgccgatg gcggcttgga cggcaatgtc gaacatttgg cgcggaatca
     1501 gctcgcgcat tttcgatgcc agctcgcggc ctcggtgaac cgcgctttga cggtggacaa
     1561 tcaggctcag cgcatcgact ttttcgccgt tgaccataat atccagctta atcaaatcag
     1621 acggttggaa ctctttgaaa tggtagtcca acgaagcata accgcgcgaa gtggatttga
     1681 gtttgtcaaa gaagtccatg acgacttcgt tcatgggcaa atcgtaagtc agcatcactt
     1741 ggcggcccat gtactgcata ttgacctgaa cgccgcgctt ttggttacac aaagtcatga
     1801 cgttgccgac gtattcctgc ggcacaagga tggtcgcggt aataatcggc tcgagtatgg
     1861 tttcgatgct gccgatgtcg ggcagtttgg acgggttttc aacttcgatt ttctcaccgt
     1921 ttttcaacac gacttcataa accaccgtcg gcgcggtggt aatcaaatcc atatcgaact
     1981 cgcgctccaa gcgttcctgc acgatttcca agtgcaacag acccaagaag ccgcaacgga
     2041 agccgaagcc caatgcctgg gaaacttcag gctcgaattt caacgaagcg tcgttaagct
     2101 gcaatttttc caaagcgtcg cgcaaggctt cgtagtcgtg gctttccacg ggataaagtc
     2161 cggcgaatac ttggctttgt acttcttgga agccgggcag cggctcagtg gcagggttgg
     2221 caaccagtgt aaccgtatcg ccaactttcg cctgacccaa ttcttttacg ccggtaatca
     2281 aaaagcccac ttcgccggct ttgagttctt gtttttgaac tgattttggt gtgaatacgc
     2341 ccagctgctc gacctgcgtt tccgccttgg tgctcataaa gcgcactttg tctttcagtt
     2401 tgatggtgcc gtttttcact cgaatcagca tgaccacgcc gacgtagttg tcaaaccacg
     2461 aatcgacaat cacggcttgc agcggcgcgt tttcatcgcc ggtcggcgca ggaattttgg
     2521 caacgatttc ttccaaaacg tcttccacgc cgatgccgct tttggcggaa cactgcaccg
     2581 cgccgaccgc atcgatgccg atgatgtctt caatttcctg ctccacgcgt tcggggtcgg
     2641 cggcgggcag gtcgattttg ttcaaaacag gcacaacctc cacgcccaaa tcaatcgcgg
     2701 tgtaacagtt cgccacggtt tgcgcttcca cgccctgcga cgcgtcaacg accaaaagcg
     2761 cgccttcgca ggcggacagc gaacgggaaa cttcgtaaga gaagtcgacg tgtcccggcg
     2821 tgtcaatcag gttgagttga tacacctgcc catcgcgtgc tttgtagttg agcgcggcgg
     2881 tttgcgcttt gatggtaatg ccgcgctctt tttcgatgtc catggaatcg agcacctgcg
     2941 tactcatttc gcgcaaatcc aagccgccgc aatattggat gaagcggtcg gcaagcgtcg
     3001 atttgccgtg gtcgatatgg gcaatgatgg agaaatttcg gatatttttc attggattgt
     3061 ttttaatgct gaacggtagg
//
LOCUS       DAWHSK010000225         3060 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.225, whole genome shotgun sequence.
ACCESSION   DAWHSK010000225 DAWHSK010000000
VERSION     DAWHSK010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.225"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            245..2467
                     /locus_tag="WIC03_001824"
     CDS             245..2467
                     /locus_tag="WIC03_001824"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEZ3884286.1"
                     /translation="MTKSTIIYTYTDEAPALATQSLLPIVQAFTRHADIDVKTSDISL
                     SGRILAAFPEYLTEAQRVPDALAELGELVKQPDANVIKLPNISASVPQLTAAIKELQS
                     KGFAVPDYPADPQTDEEKAVRERYDRIKGSAVNPVLREGNSDRRAPKAVKNFAKKNPH
                     SMGAWAKDSKTHVATMQSGDFFHNEQSVTVPEATSVSIVFTDKQGNKKELRAPVALKA
                     GEIIDATVMSKKALLAFLAEQVKDAKAKGVLFSLHMKATMMKVSDPIIFGHAVKVFFA
                     PVFEKFGGKLAAAGVNVNNGFGNLLANLDKLDADTRAAVEAEIAAVYAANPDLAMVDS
                     DKGITNLHVPSDVIVDASMPAMIRNSGRMWDKNGKAQDTKAVIPDSSYAGVYQATIDF
                     CREHGAFDPTTMGTVPNVGLMAQAAEEYGSHNKTFEIEADGQVQVVDAAGKVLMQHDV
                     EAGDIWRMCQTKDAPVKDWVQLAVNRARLSNTPAVFWLDENRPHDKSLLAKVKAYLAE
                     LDTNGLDIRVLAPEEAAKFSLGRLKNGEDTISVTGNVLRDYLTDLFPILELGTSAKML
                     SIVPLMNGGGMFETGAGGSAPKHVQQFLEENHLRWDSLGEFLALAVSFEHLAQKTGNT
                     KAQVLADTLDAATEKLLLNDKSPKRKTGELDNRGSHFYLTLYWAQELAAQDKDAELKA
                     AFAPLAKSLAENEAKIVEELSAVQGKAVDIGGYYAANPEKAAQAMRPSATFNQALSTL
                     "
     gene            2610..2918
                     /locus_tag="WIC03_001825"
     CDS             2610..2918
                     /locus_tag="WIC03_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884287.1"
                     /translation="MKTVPTILISILLATALPASAHGIHKSKPLTMDELPPICQQYFK
                     RAETCYNKAGNKANFARNNTKFLFQALPAADLGQRKQMCQIAMDSFAEKTRISNSNAV
                     "
     gene            2908..3060
                     /locus_tag="WIC03_001826"
     CDS             2908..3060
                     /locus_tag="WIC03_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884288.1"
                     /translation="MPSETMISDGILTSKRTSLIKPAFPASLTRTGLNEVTALTIMAS
                     SHSQKL"
BASE COUNT          871 a          913 c          674 g          602 t
ORIGIN      
        1 aatttaaacc actataaaat caatttcacc gaaaatatgc cgccgcacat caggcacagc
       61 acctccgacc gccgccaaac gcgggaaaac catttctccc ttcctttcac gccgccgcgt
      121 tcaaacacgc caagatcgaa caaagtgcga acaaatccgc cgaaatatgc cataataggc
      181 ttagcacgtt tcttctcgcg atgtgcccga aaagaaaaac ttaacctaca tagagaataa
      241 tcccatgact aaatccacca ttatctatac ctacaccgac gaagctcctg cattggccac
      301 tcaatccctg ttgccaatcg tgcaggcgtt tacccgccat gccgatattg atgtcaagac
      361 cagcgacatc tctctctccg gccgtatttt ggcggcgttt ccagaatacc tgaccgaagc
      421 gcaacgcgta cctgatgcgc ttgccgaatt gggcgaactg gtgaaacaac ccgatgcaaa
      481 cgtaatcaaa ctgccgaaca tcagcgcatc cgtacctcaa ctgactgccg cgattaaaga
      541 attgcagtct aaaggctttg ccgttcccga ctatcctgcc gaccctcaaa ccgatgaaga
      601 aaaagccgta cgcgaacgct acgaccgcat caaaggcagc gcggtaaacc ctgtcctgcg
      661 tgaaggcaac tccgaccgcc gcgcccccaa agcagtgaaa aactttgcca aaaaaaatcc
      721 gcacagtatg ggcgcgtggg cgaaagactc caaaacccac gttgccacca tgcaaagcgg
      781 cgactttttc cataacgaac aatccgttac cgtacccgaa gcgacttccg tatccatcgt
      841 gttcaccgac aaacaaggca acaaaaaaga gctgcgcgcg cctgttgccc tgaaagccgg
      901 cgaaatcatc gacgcaaccg tgatgagcaa aaaagccctg ctcgccttcc ttgccgaaca
      961 agtgaaagac gcgaaggcaa aaggcgtatt gttctcactg catatgaaag ccacgatgat
     1021 gaaagtgtcc gacccgatta tcttcggaca cgccgtcaaa gtgttcttcg cgcctgtatt
     1081 tgaaaaattc ggcggcaaac tggctgccgc aggtgtcaac gtcaacaacg gtttcggcaa
     1141 cctgcttgcc aatctggaca aactggatgc ggacacccgc gccgccgtcg aagctgaaat
     1201 cgccgccgtt tacgccgcca accctgattt ggcgatggtt gattccgaca aaggcatcac
     1261 caacctgcac gttcccagcg atgtcatcgt cgacgcttct atgcctgcga tgattcgtaa
     1321 ttccggccgt atgtgggaca aaaacggcaa agcacaagac accaaagccg tgattcccga
     1381 cagcagctat gccggcgttt accaagcaac catcgacttc tgccgcgaac acggcgcatt
     1441 tgacccgaca accatgggta ctgtgcccaa cgtcggactg atggcgcaag cggcggaaga
     1501 atacggctcg cacaacaaaa cctttgaaat cgaagccgac ggtcaggttc aagtagttga
     1561 tgcggcagga aaagtcctga tgcagcacga cgttgaagca ggcgacatct ggcgtatgtg
     1621 ccaaaccaaa gacgctccgg ttaaagactg ggtacaactc gccgtcaacc gcgcccgcct
     1681 gagcaatacg cccgccgtgt tctggctcga cgaaaaccgt ccgcatgaca agagcctgct
     1741 cgccaaggtt aaagcctacc ttgccgaact ggataccaac ggcctcgaca tccgcgtcct
     1801 cgctcccgaa gaagccgcca agttcagcct gggccgtctg aaaaacggcg aagacaccat
     1861 ctccgtaacc ggtaatgtct tgcgcgacta cctgaccgac ttgttcccga ttttggaact
     1921 cggcacaagc gcgaaaatgc tgtctatcgt tccattgatg aacggcggcg gtatgtttga
     1981 aaccggcgcg ggcggttctg caccgaaaca cgtccaacaa ttccttgaag aaaaccactt
     2041 gcgttgggac tctttgggcg aattcctcgc gctcgccgta tcgtttgaac atttggcaca
     2101 aaaaaccggc aacaccaaag cccaagtcct cgccgacact ttggatgccg ccaccgaaaa
     2161 actgctgcta aacgacaaat cgcccaaacg caaaaccggc gaactcgaca accgcggcag
     2221 ccatttctac ctcaccctct actgggcgca agaattggcg gcgcaagaca aagatgccga
     2281 attgaaagcc gcatttgcgc cgttagccaa atcgttggcg gaaaacgaag ccaaaatcgt
     2341 tgaagaactc tctgccgtac aaggcaaagc ggtcgacatc ggcggctact acgccgccaa
     2401 tcctgaaaaa gcggcacaag cgatgcgccc aagcgcaacc tttaatcagg cactaagcac
     2461 cttataagca caaaggtaaa aatgccgtct gaacattcgt cgttcagacg gcattagtta
     2521 ttcctatctg cctgattatg attaaacgcc gcacccattg gatataaatc atataatctt
     2581 tagtttttac cctaggaaaa gacaataata tgaaaactgt tccaaccatc ctgattagca
     2641 tccttttggc aaccgccctt cctgcatccg cacacggtat acataagagc aagcccctga
     2701 ctatggacga gctgccgccg atttgccaac aatatttcaa acgcgccgaa acctgttaca
     2761 acaaagccgg caacaaggca aattttgcac gcaacaacac taaattccta ttccaagccc
     2821 tgcctgctgc cgatttggga cagcgtaagc aaatgtgcca aatcgctatg gattcgtttg
     2881 cagaaaaaac ccgcatcagc aatagcaatg ccgtctgaaa caatgatttc agacggcatt
     2941 ctcacatcaa aacgcactag cttgataaaa cccgccttcc ccgcatccct gacccgaaca
     3001 ggattaaacg aagtaacggc tttaaccatt atggcaagtt cccattctca aaaattatag
//
LOCUS       DAWHSK010000226         3041 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.226, whole genome shotgun sequence.
ACCESSION   DAWHSK010000226 DAWHSK010000000
VERSION     DAWHSK010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.226"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..152
                     /locus_tag="WIC03_001827"
     CDS             <1..152
                     /locus_tag="WIC03_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF815 domain-containing protein"
                     /protein_id="HEZ3884289.1"
                     /translation="WLEDFGVPYDETAQMAALQWAQTRGSRSGRSAWQFACDWAGRLP
                     KQRAL"
     gene            complement(165..599)
                     /locus_tag="WIC03_001828"
     CDS             complement(165..599)
                     /locus_tag="WIC03_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HEZ3884290.1"
                     /translation="MPLPAPCRFAKPAASFLSMALLSCQLSHAATAYIPPNDFQPNCD
                     IRRLGLTQSQHNELRKIRTAFKMAGDRARLKVMHSEHSRRRSVVEIISSDVFNRNEAR
                     DYVESRYLSGMDFAVDELEIQHRFFHILTPQQQQMWLSSCLK"
     gene            745..1473
                     /gene="rpe"
                     /locus_tag="WIC03_001829"
     CDS             745..1473
                     /gene="rpe"
                     /locus_tag="WIC03_001829"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEZ3884291.1"
                     /translation="MTAYRIAPSILSADFARLGEEVESVIAAGADLIHFDVMDNHYVP
                     NLTFGPMVCAALKPYASVPIDVHLMVEPVDDLIQSFAKAGASIITFHPEASRHIDRSL
                     SLIRDMGCQAGLVLNPATPVYLLENVLDRLDMVLLMSVNPGFGGQSFIPHTLEKIRRV
                     RAMLDRYEAQSGRHIAIEVDGGIKTDNIAAVARAGADTFVAGSAIFGKPDYKAVIDAM
                     RAELEKSGRLNLAPMIEIEMPSEN"
     gene            complement(1496..2527)
                     /gene="ruvB"
                     /locus_tag="WIC03_001830"
     CDS             complement(1496..2527)
                     /gene="ruvB"
                     /locus_tag="WIC03_001830"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEZ3884292.1"
                     /translation="MLQTDNLTAAQPQRIVAAQTASAQEELLERALRPKTLDDYIGQH
                     KAKEQLAIFIQAAKKRGEALDHVLLFGPPGLGKTTLAHIIAKELGVNLRQTSGPVLER
                     AGDLAALLTNLDPHDVLFIDEIHRLSPVVEEILYPALEDYRLDIMIGEGPAARSVKID
                     LPPFTLIGATTRAGMLTNPLRDRFGIVSRLEFYENRDLTTIVSRSAQLLQLDMSEEGA
                     EEIAKRSRGTPRIANRLLRRVRDFADVKNNGTIDGGIADAALSMLDVDAQGLDVMDRK
                     FLEAVLHKFGGGPVGLDNVAAAIGESTDTIEDVIEPYLIQQGFLQRTPRGRMATERAY
                     LHFGLPVEK"
     gene            complement(2609..2917)
                     /locus_tag="WIC03_001831"
     CDS             complement(2609..2917)
                     /locus_tag="WIC03_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884293.1"
                     /translation="MTLKTDLLLKINNEDYQRLILKHISEFSEGEIRLLNEILEKFNF
                     DVVQAQALAQAVMQQVRFDPNAYHIDSDDEDTTGICPHCINPPMPPLRDYLVWRETRG
                     "
BASE COUNT          639 a          714 c          963 g          725 t
ORIGIN      
        1 attggctgga agatttcggc gtgccgtatg atgaaaccgc acagatggcg gcattgcagt
       61 gggcgcagac gcggggcagc cgttcgggac gttccgcgtg gcagtttgcc tgcgactggg
      121 cgggcaggct gccgaaacaa cgggcgttgt gagcgtttcg gggattattt gaggcaggaa
      181 gaaagccaca tttgctgctg ttgcggtgtg aggatatgga agaaccggtg ttggatttcc
      241 aattcgtcca ccgcaaaatc cataccggac aaatagcggc tttcgacata atcgcgcgcc
      301 tcgttccgat taaaaacatc cgaggaaatg atttcgacga cagaccggcg gcggctgtgt
      361 tcggaatgca taaccttcaa acgcgccctg tcgcccgcca ttttgaaggc ggtgcggatt
      421 ttacgcagct cattgtgctg actttgggtc agcccgagtc ggcgtatgtc gcagttcggt
      481 tgaaaatcgt tcggggggat ataagccgtg gcggcgtggg aaagctgaca ggaaagcaaa
      541 gccatactta aaaaagaggc ggcaggtttg gcaaaacggc agggagcagg cagtggcaca
      601 gcttttcctt taatccagat atttcggaca atatacttga ttgccgtgtg aaagatggta
      661 ttatggcagt taacttttgt taataatgct gtttcggggc tttcccgcgt tgagtccgcc
      721 cgtaaaggca ttgaaggaaa aaatatgacc gcctaccgta tcgcacccag tattttgtcg
      781 gcagactttg cccgcctcgg agaagaggtg gaaagcgtca ttgcggcagg tgcagatctg
      841 atccattttg acgtgatgga caaccattat gtgccgaacc tgaccttcgg ccctatggtt
      901 tgcgcggcgt tgaagcctta tgcaagcgtg ccgatcgatg tgcatttgat ggtcgaaccc
      961 gttgacgacc tgattcagtc gtttgccaaa gcaggagcat cgattatcac ttttcatccc
     1021 gaagcgagcc gccatatcga ccgcagcttg agcctgattc gtgatatggg ctgtcaggcg
     1081 gggctggtgt tgaatccggc aacgcccgta tatctgttgg aaaacgtatt ggacaggctg
     1141 gatatggttt tgctgatgtc ggtcaacccc ggattcggcg gacaaagctt catcccgcac
     1201 acccttgaaa aaatccgccg ggtgcgggcg atgctggatc ggtacgaagc acaaagcggg
     1261 cggcacatcg ccatcgaagt ggacggcggc atcaaaaccg acaatattgc cgccgttgcc
     1321 cgagctggtg cggatacctt tgttgccggt tctgcgattt tcggcaaacc agactataag
     1381 gcggtgattg atgcgatgag ggcggaactg gaaaaaagcg gcaggctgaa ccttgcgcca
     1441 atgattgaaa tagaaatgcc gtctgaaaat tagctctgtt tcagacggca ttgcgttatt
     1501 tttcgacggg cagcccgaaa tgcaggtagg cgcgttcggt cgccatcctg ccgcgcgggg
     1561 tgcgttgcag gaagccttgt tggataaggt agggttcgat aacgtcttcg atggtgtctg
     1621 tagattcgcc gatggcggcg gcaacattgt ccaaaccgac cgggccgccg ccgaatttgt
     1681 gcaaaacggc ttcgagaaat ttcctgtcca tcacgtccag cccctgcgcg tccacgtcca
     1741 gcatacttaa agcggcatcg gcgatgccgc cgtcgattgt gccgttgttt ttcacgtcgg
     1801 cgaaatcgcg cacgcgtcgc aacaggcggt tggcgatgcg cggcgtaccg cggctgcgtt
     1861 tggcgatttc ttccgcgcct tcttcggaca tatcgagctg caacagttgt gccgaacggc
     1921 tgacgatggt ggtaaggtct cggttttcgt aaaactcaag gcgggagacg atgccgaagc
     1981 ggtcgcgcaa cggattggtc agcataccgg cgcgggtggt cgcgccgatg agcgtgaagg
     2041 gcggcaggtc gattttaacg gaacgggcgg cgggcccttc gcctatcata atgtcgagcc
     2101 ggtagtcttc aagcgcgggg tagaggattt cttcgacaac agggctgagg cggtggattt
     2161 cgtcgataaa caatacgtcg tgcggatcaa ggttggtcaa aagggcggcg aggtcgccgg
     2221 cgcgttcgag gacggggccg ctggtttggc gcaaatttac gcccaattct ttggcgatga
     2281 tgtgcgccag tgtggtttta cccagcccgg gcggaccgaa aagcaagacg tggtcgagtg
     2341 cttcgccgcg ctttttggcg gcttggatga aaatggcgag ctgttctttg gctttatgct
     2401 gcccgatata gtcgtccagc gttttggggc ggagggcgcg ttcgagcagt tcttcctgtg
     2461 cggaggcggt ttgggcggca acaatgcgtt gcggttgagc ggcagtcagg ttgtcggttt
     2521 gcagcatagt gttccctttg tcgggtatgc cgtctgaacg gtcggcggcg tttcagacgg
     2581 cattgaaaag ataacggtca aaagcgtttt acccgcgcgt ttcgcgccaa acgagatagt
     2641 cgcgcagggg cggcataggc gggttgatgc agtgtgggca gatgcctgtg gtgtcttcgt
     2701 cgtcgctgtc gatgtggtag gcgttggggt cgaagcggac ttgctgcatt accgcctgcg
     2761 ccaatgcctg cgcctgaacg acgtcgaaat tgaatttttc aaggatttcg ttcaacaggc
     2821 ggatttcgcc ctcgctgaat tccgagatgt gtttgaggat gaggcgttga taatcttcgt
     2881 tgttgatttt aagcaataaa tcggttttca gggtcatgat tttatgtcgg gggcggaatg
     2941 gctgaagcgt gaattatagc ggatatggcg gcggctgttg caaagcttgg attgggtatc
     3001 gtcctcaaaa ctttatagtg gattaacaaa aaccagtaca g
//
LOCUS       DAWHSK010000227         3022 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.227, whole genome shotgun sequence.
ACCESSION   DAWHSK010000227 DAWHSK010000000
VERSION     DAWHSK010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.227"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            67..846
                     /locus_tag="WIC03_001832"
     CDS             67..846
                     /locus_tag="WIC03_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HEZ3884294.1"
                     /translation="MAASPEAKFTEEKILWVKHHTPKLITFAISRPESYRFKAGQFSR
                     LGFYEGEGFIWRAYSIVSAEYADTLEYFAVLIQDGPMSARFAKMQQGDTILLDKNATG
                     FLLPERFPDGKDLVMLCTGSGIAPFLSILEQPEIRQRFDTVNLIHSVSFPEELIFNDR
                     LAALSEHPLVGEYRHSFHFVPVTTRAANPSGLSGKRIPELLKNSSIEQALHTKFTPES
                     TRFMICGNPEMVKDTFQTLLDMGYAMHRNRIPGQIMMENGF"
     gene            complement(1057..2469)
                     /locus_tag="WIC03_001833"
     CDS             complement(1057..2469)
                     /locus_tag="WIC03_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEZ3884295.1"
                     /translation="MLFEFKENKMIVASRWPLLEKVFLRFGMPVAVVDLESTGGNLYE
                     DRVTEVALVKFEQGRVVRHEWLVNPQKPIPQFVAGLTGISDGMVADAPVFAEIAGELF
                     SVLKGCVLVAHNSRFDYTFLKHEFHRVGIGFSSPALCSVQLSRCLYPQFYKHSLDSII
                     ERLGIVVEDRHRAMADVSALCDYLEYSLSEHGVEAWIRQCFRLMNPKPLPAALPERLR
                     EQLYGLPDGMGVLACFDGGGKVNYIGTFERVYSEVSALLDSGKAPFDWCNTEEVRFFP
                     ALGSLHAYKIKAELVGRYHSGCYVSAKNLLKTFTTVRFEKGSDGMLNAKTAALKNGVT
                     DNPPTGLFANKKAARRALSSWAETYGLCPASAGILPDGYAEDEPCPVYVSGRCDKACG
                     RSDEQVLAFAHKLPILDWGKMHEVEITETDPLTGEKVVLHGMGGALEMGDGLWYFDKD
                     LPDAFKAKFKTDRKNIKEIG"
     gene            2560..2636
                     /locus_tag="WIC03_001834"
     tRNA            2560..2636
                     /locus_tag="WIC03_001834"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2594..2596,aa:Arg,seq:acg)
     gene            2654..2728
                     /locus_tag="WIC03_001835"
     tRNA            2654..2728
                     /locus_tag="WIC03_001835"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2687..2689,aa:Glu,seq:ttc)
BASE COUNT          831 a          911 c          569 g          711 t
ORIGIN      
        1 ccgtcaatgc ggaaaaaaac acccaaattg gctataatcc cgacaaacac actcaaggac
       61 aacaacatgg cagcctcgcc cgaagcaaaa ttcactgaag aaaagatttt gtgggtcaaa
      121 caccacacgc cgaaactcat cactttcgcc atcagccgtc ccgaatccta ccgttttaaa
      181 gccggacagt tctcccgact cggtttctac gaaggggaag gctttatttg gcgtgcctac
      241 tccatcgttt ccgcagaata tgcagacacg ctcgaatatt ttgccgtact catccaagac
      301 ggccccatgt cggcccgttt cgccaaaatg caacagggcg acaccatcct gctcgataaa
      361 aatgccaccg gcttcctcct gcccgaacgc ttccccgacg gcaaggattt ggtgatgctc
      421 tgcaccggct ccggcatcgc ccccttcctt tccattctcg aacaacctga aatccgacag
      481 cgtttcgata ccgtcaacct gatacattcc gtatcttttc ccgaagaatt gattttcaac
      541 gaccggctcg ccgcattgtc cgaacatccc ctggtaggcg aatacagaca ctcgttccat
      601 ttcgtccctg ttaccacccg tgccgccaac ccctcgggct taagcggaaa acgcattccg
      661 gaactcttaa aaaacagcag catcgaacag gcgctgcata ccaagttcac cccggaatcc
      721 acacggttta tgatttgcgg caacccggaa atggtcaaag atactttcca aacgctgctt
      781 gacatgggtt acgccatgca ccgcaaccgc attcccggtc aaatcatgat ggaaaacggc
      841 ttctaaaaac caccctgctt gtccgatgcc ttcggatgga cggtcaaacc gacacggcac
      901 gaaaaccgcg tcggcaaaaa tgccgtctga aaaaattcag acggcatctt cggacacatt
      961 acctgcaaac ggcaacacat tccaacccaa accgattagg caatcaatac gaacggctgt
     1021 ttacatactt accggctttc atcaaccggt atcgatttaa ccgatttcct taatattttt
     1081 cctgtccgtt ttaaacttcg ccttaaacgc atccggtaaa tctttatcga aataccaaag
     1141 cccgtcaccc atttccaatg cgccgcccat tccatgcaga acaacttttt cccccgttaa
     1201 aggatcagtt tcggtaattt ctacctcatg catttttccc caatccaaaa taggcaactt
     1261 gtgtgcaaac gccaaaacct gttcgtcaga acggccgcac gccttatcgc atcggcctga
     1321 aacatatacg ggacaaggct catcctctgc ataaccgtcc ggaaggatac cggctgaggc
     1381 aggacacaat ccgtatgttt ccgcccacga cgacaaagcc cgtctcgccg cctttttatt
     1441 ggcaaataat ccggtaggcg gattatccgt cacaccgttt ttcaaagccg ctgttttcgc
     1501 attcaacatg ccgtctgaac ctttttcaaa cctgacggtc gtaaatgttt taagcagatt
     1561 tttggcagat acataacaac ccgaatgata acgtccgacc aattccgctt taatcttata
     1621 tgcatgcagg ctgcccaatg caggaaaaaa acggacttcc tccgtattgc accaatcaaa
     1681 cggggctttt ccggaatcca ataaagccga aacctcgcta tatacccgtt caaacgtacc
     1741 gatgtaattt acttttcctc cgccgtcgaa acaggccagc acccccatac cgtcaggcaa
     1801 accgtacaac tgttccctca accgttcggg cagcgcggca ggcaacggtt tcggattcat
     1861 caaacggaaa cactgcctga tccatgcctc gaccccgtgt tccgacagac tgtattccaa
     1921 ataatcgcac aatgccgata catccgccat cgcacgatgc ctgtcttcca caacaatccc
     1981 caacctttcg atgatactgt ccaggctgtg cttgtaaaat tgcggataca gacaccggga
     2041 cagctgcaca ctgcacaaag caggcgatga aaatccgata cccacacgat gaaactcatg
     2101 ctttaaaaac gtatagtcga aacggctgtt atgtgcaacc agcacacaac ccttcaatac
     2161 cgaaaacaac tcgccggcaa tctctgcaaa aacaggcgca tcggcaacca tgccgtctga
     2221 aatccccgtc agccccgcca caaactgcgg aatcggtttt tgaggattaa ccaaccactc
     2281 atgcctcacc acccttccct gctcaaactt gaccaaagcc acttcggtta ccctgtcttc
     2341 atacagattg ccgcccgtcg attccaaatc gaccacggca acaggcattc caaaccgtaa
     2401 aaataccttt tccagcaagg gccagcggga agcaacaatc attttattct ctttaaattc
     2461 aaacaacaaa ccgatatttt acacttttaa ggcatttcat ccaacaaaac aattgacaga
     2521 atccgatgat taccctaaaa ttcaaatctt tcttgcagtg cacccgtagc tcagttggat
     2581 agagtatctg gctacgaacc agagggtcgg gcgttcgaat cgctccgggt gcgccagtaa
     2641 gaaaatacaa tatgcgccca tcgtctagcg gttaggacat cgccctttca cggcggtaac
     2701 cggggttcga ttccccgtgg gcgtgccaaa ttctaaatcc ccgagattat cgctcgggga
     2761 ttttttattg tctcagcaac tcgttaccat atctttacct acccccttca tcagaatctc
     2821 agacgtaatc gaatcatatt caaacctttg ccgtgcaaac cgatatccca taaccggatg
     2881 cggtgtccgt ccaacatttt acccgattga aacgcctgat atattgcacc ccatcaacgt
     2941 ggcattactt ttcttaacaa tcccctttga cagcaactga ctagggcttt tttatgccat
     3001 catcaaattt atagtggatt aa
//
LOCUS       DAWHSK010000228         2978 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.228, whole genome shotgun sequence.
ACCESSION   DAWHSK010000228 DAWHSK010000000
VERSION     DAWHSK010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.228"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            113..2878
                     /locus_tag="WIC03_001836"
     CDS             113..2878
                     /locus_tag="WIC03_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ3884296.1"
                     /translation="MRSSFRLKPICFYLMGVMLYHHSYAEDAGRAGSEAQIQVLEDVH
                     VKAKRVPKDKKVFTDARAVSTRQDIFKSSENLDNIVRSIPGAFTQQDKSSGIVSLNIR
                     GDSGFGRVNTMVDGITQTFYSTSTDAGRAGGSSQFGASVDSNFIAGLDVVKGSFSGSA
                     GINSLAGSANLRTLGVDDVVQGNNTYGLLLKGLTGTNSTKGNAMAAIGARKWLESGAS
                     VGVLYGHSRRSVAQNYRVGGGGQHIGNFGAEYLERRKQRYFVQTGGLKFNSDSGKWER
                     DFQRQFWKHKLYKKYDDPQELQKYIEGQDKDWRENLAPQYDITPIDPSSLKQQSAGNL
                     FKLEYDGVFNKYTAQFRDLNTKIGSRKIINRNYQFNYGLSLNSYANLNLTAAYNSGRQ
                     KYPKGSKFTGWGLLKDFETYNNAKILDLNNTATFRLPRETELQTTLGFNYFHNEYGKN
                     RFPEELGLFFDGPDQDNGLYSYLGRFKGDKGLLPQKSTIVQPAGSQYFNTFYFDAALK
                     KDIYRLNYSTNTVGYRFGGEYTGYYGSDDEFKRAFGENSPTYKKHCNRSCGIYEPVLK
                     KYGKKRANNHSVSISADFGDYFMPFASYSRTHRMPNIQEMYFSQIGDSGVHTALKPER
                     ANTWQFGFNTYKKGLLKQDDTLGLKLVGYRSRIDNYIHNVYGKWWDLNGNIPSWVSST
                     GLAYTIQHRNFKDKVHKHGFELELNYDYGRFFTNLSYAYQKSTQPTNFSDASESPNNA
                     SKEDQLKQGYGLSRVSALPRDYGRLEVGTRWLGNKLTLGGAMRYFGKSIRATAEERYI
                     DGTNGGNTSNVRQLGKRSIKQTETLARQPLIFDFYAAYEPKKNLIFRAEVKNLFDRRY
                     IDPLDAGNDAATQRYYSSFDPKDKDEDVTCNADGLCNGKYGGTSKSVLTNFARGRTFL
                     ITMSYKF"
BASE COUNT          844 a          748 c          727 g          659 t
ORIGIN      
        1 ccaattgaaa tcttcagacg gtatatcgaa tttacacttt ttaatgttta tgccgcctaa
       61 aaaaatgcta atatatttct taattgtctg actgtttatt gttgaggaaa atatgagatc
      121 ttctttccgg ttgaagccga tttgttttta tcttatgggt gttatgctat atcatcatag
      181 ttatgccgaa gatgcagggc gcgcgggcag cgaggcgcag atacaggttt tggaagatgt
      241 gcacgtcaag gcgaagcgcg taccgaaaga caaaaaagtg tttaccgatg cgcgtgccgt
      301 atcgacccgt caggatatat tcaaatccag cgaaaacctc gacaacatcg tacgcagcat
      361 acccggtgcg tttacacagc aagataaaag ctcgggcatt gtgtctttga atattcgcgg
      421 cgacagcggg ttcgggcggg tcaatacgat ggtggacggc atcacgcaga ccttttattc
      481 aacttctacc gatgcgggca gggcaggcgg ttcatctcaa ttcggtgcat ctgtcgacag
      541 caattttatt gccggactgg atgtcgtcaa aggcagcttc agcggctcgg caggcatcaa
      601 cagccttgcc ggttcggcga atctgcggac tttaggcgtg gatgacgtcg ttcagggcaa
      661 taatacctac ggcctgctgc taaaaggtct gaccggcacc aattcaacca aaggtaatgc
      721 gatggcggcg ataggtgcgc gcaaatggct ggaaagcgga gcatctgtcg gtgtgcttta
      781 cgggcacagc aggcgcagcg tggcgcaaaa ttaccgcgtg ggcggcggcg ggcagcacat
      841 cggaaatttt ggcgcggaat atctggaacg gcgcaagcag cgatattttg tacaaacagg
      901 ggggttgaaa ttcaattccg acagcggaaa gtgggagcgg gatttccaaa ggcaattctg
      961 gaaacacaag ttgtataaaa aatacgatga cccccaagaa ctgcaaaaat acatcgaagg
     1021 tcaagataaa gactggcggg aaaacctggc gccgcaatac gacatcaccc ccatcgatcc
     1081 gtccagcctg aagcagcagt cggcaggcaa tctgtttaaa ttggaatacg acggcgtatt
     1141 caataaatac acggcgcaat ttcgcgattt aaacaccaaa atcggcagcc gcaaaatcat
     1201 caaccgcaat tatcaattca attacggttt atctttaaac tcatatgcca acctcaatct
     1261 gaccgcagcc tacaattcgg gcaggcagaa atatccgaaa gggtcgaagt ttacaggctg
     1321 gggactttta aaggattttg aaacctacaa caacgcgaaa atcctcgacc tcaacaacac
     1381 cgccaccttc cggctgcccc gcgaaaccga gttgcaaacc actttgggct tcaattattt
     1441 ccacaacgaa tacggcaaaa accgctttcc tgaagaattg gggctgtttt tcgacggtcc
     1501 tgatcaggac aacgggcttt attcctattt ggggcggttt aagggcgata aagggctgct
     1561 gccccaaaaa tcaaccattg tccaaccggc cggcagccaa tatttcaaca cgttctactt
     1621 cgatgccgcg ctcaaaaaag acatttaccg cttaaactac agcaccaata ccgtcggcta
     1681 ccgtttcggc ggcgaatata cgggctatta cggctcggat gacgaattta agcgggcatt
     1741 cggagaaaac tcgccgacat acaagaaaca ttgcaaccgg agctgcggga tttatgaacc
     1801 cgtattgaaa aaatacggca aaaagcgcgc caacaaccat tcggtcagca ttagtgcgga
     1861 cttcggcgat tatttcatgc cgttcgccag ctattcgcgc acacaccgta tgcccaacat
     1921 ccaagaaatg tatttttccc aaatcggcga ctccggcgtt cacaccgcct taaaaccaga
     1981 gcgcgcaaac acttggcaat ttggcttcaa tacctataaa aaaggactgt taaaacaaga
     2041 tgatacatta ggattaaaac tggtcggcta ccgcagccgc atcgacaact acatccacaa
     2101 cgtttacggg aaatggtggg atttgaacgg gaatattccg agctgggtca gcagcaccgg
     2161 gctcgcctac accatccaac accgcaattt caaagacaaa gtacacaaac acggttttga
     2221 gttggagctg aattacgatt atgggcgttt tttcaccaac ctttcttacg cctatcaaaa
     2281 aagcacgcaa ccgaccaact tcagcgatgc gagcgaatcg cccaacaatg cgtccaaaga
     2341 agaccaactc aaacaaggtt atgggttgag cagggtttcc gccctgccgc gagattacgg
     2401 acgtttggaa gtcggtacgc gctggttggg caacaaactg actttgggcg gcgcgatgcg
     2461 ctatttcggc aagagcatcc gcgcgacggc agaagaacgc tatatcgacg gcaccaacgg
     2521 gggaaatacc agcaatgtcc ggcaactggg caagcgttcc atcaaacaaa ccgaaaccct
     2581 tgcccgccag cctttgattt ttgattttta cgccgcttac gagccgaaga aaaaccttat
     2641 tttccgcgcc gaagtcaaaa atctgttcga caggcgttat atcgatccgc tcgatgcggg
     2701 caatgatgcg gcaacgcagc gttattacag ctcgttcgac ccgaaagaca aggacgaaga
     2761 cgtaacgtgt aatgctgatg gcttgtgcaa cggcaaatac ggcggcacaa gcaaaagcgt
     2821 attgaccaat tttgcacgcg gacgcacctt tttgataacg atgagctaca agttttaaag
     2881 gcagcccgcg ttttgtggaa aaccgcaatg ccgtctgaaa gcccttcaga cggcatttgt
     2941 ttccccaaac gcatcatcct gccgcaagcc tatgccaa
//
LOCUS       DAWHSK010000229         2972 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.229, whole genome shotgun sequence.
ACCESSION   DAWHSK010000229 DAWHSK010000000
VERSION     DAWHSK010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2972
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.229"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(122..598)
                     /gene="greA"
                     /locus_tag="WIC03_001837"
     CDS             complement(122..598)
                     /gene="greA"
                     /locus_tag="WIC03_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEZ3884297.1"
                     /translation="MQKIPLTVRGAELLKQELQQLKSVARPEVIEAIAEARSHGDLSE
                     NAEYEAAKERQGFIEGRISELEHKLSVAHIINPTEIHAEGKIVFGTTVTLEDLETEEH
                     VIYQIVGEDEADIKQGKIYVGSPIARALIGKEEGDTAEVQAPGGVREYDIIEVRYI"
     gene            994..2295
                     /gene="aroA"
                     /locus_tag="WIC03_001838"
     CDS             994..2295
                     /gene="aroA"
                     /locus_tag="WIC03_001838"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEZ3884298.1"
                     /translation="MTESVRLPVARLKPSTVALPGSKSISNRTLLLAALSDNACEIHS
                     LLKSDDTDRMLEALDKLGVQIEYLAEDRLKVHGTGGRFPNRTADLFLGNAGTAFRPLT
                     AALAVLGGDYHLHGVPRMHERPIGDLVDALRIAGADVEYLGKEHYPPLHIGERQDNGE
                     RVIPIKGNVSSQFLTALLMALPLTGQAFEIRMVGELISKPYIDITLKLMAQFGVQVIN
                     EGYRVFKIPADAHYHAPEHLHVEGDASSASYFLAAGLIAATPVRVTGIGANSIQGDVA
                     FARELEKIGADVVWGENFVEVSRPKERAVQSFDLDANHIPDAAMTLAIVALATRQTCT
                     LRNIGSWRVKETDRIAAMANELRKLGAKVVEEAEAIHITPPETLTPDAVIDTYDDHRM
                     AMCFSLVSLLGVPVVINDPKCTHKTFPTYFDVFSSLTETAE"
     gene            complement(2338..2871)
                     /locus_tag="WIC03_001839"
     CDS             complement(2338..2871)
                     /locus_tag="WIC03_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEZ3884299.1"
                     /translation="MFPPDKTLFLCLSALLLASCGTTSGKHRQPKPKQTVRQIQAVRI
                     SHIDRTQGSQELMLHSLGLIGTPYKWGGSSTATGFDCSGMIQFVYKNALNVKLPRTAR
                     DMAAASRKIPDSRLKAGDLVFFNTGGAHRYSHVGLYIGNGEFIHAPSSGKTIKTEKLS
                     TPFYAKNYLGAHTFFTE"
BASE COUNT          631 a          808 c          806 g          727 t
ORIGIN      
        1 aagcaccaag tgaatcggtt tcgtactatc tgtactgtct tcggcttcgt cgccttgtcc
       61 tgatttttat taatccacta taatttcctg cgtgtgtcgg gtgtatcgaa atcaagccga
      121 atcaaatata tcggacttcg ataatgtcgt attcgcgcac gccgcccggg gcttggactt
      181 ccgccgtatc cccctcttcc ttgccgatta aggcgcgggc gatgggtgag ccgacataga
      241 ttttgccctg tttgatgtcg gcttcgtctt cgccgacaat ttgatagata acgtgttctt
      301 ccgtttccaa atcttccagc gtaaccgtcg taccgaacac gattttgcct tcggcgtgga
      361 tttcggtcgg attgatgatg tgggcaacgg aaagtttgtg ttccagctca gaaatgcggc
      421 cctcgataaa gccttggcgt tctttggcgg cttcgtattc ggcgttttcg gacaaatcgc
      481 cgtgcgaacg ggcttcggca atcgcttcga tcacttcggg acgcgccacg cttttgagct
      541 gctgcaattc ctgtttcagc aattccgcac cgcgtacggt cagggggatt ttttgcatcg
      601 gtcttttttc tccatattcc ggcacaccgg tttgcggcag caagcatacc gcgtaccgtc
      661 ttgttttgtg cgtccggata ttaaaataaa aatacaagcc gcccggaaaa tcggcggctt
      721 gtctgtcgtt gaacagcggc tattctacca aattctatga aattggcaat cgtgccgtgc
      781 cgccggcaaa cgcgccatgt ccgcaacaaa agctgaaaat atgccgtcaa agaaatttta
      841 gaaacaaaaa atttaaaaat aatcaatttt cagcataaaa aaccacattt acggacttta
      901 aaaccgaaaa tgccaagcct gagatttttc atacagcatt tgcaccagta taatgcaggc
      961 tgtttttatc tttaataata ttgacgtttt gccatgaccg aatccgtccg cctccccgtc
     1021 gcccgtctca aaccttccac cgtcgccctg cccggctcca aaagcatcag caaccgcacc
     1081 ctgctgcttg ccgccttgtc cgacaatgct tgcgaaatcc attccctgct caaatccgac
     1141 gataccgacc gtatgctcga agcactcgat aaactcggcg ttcaaatcga atatcttgcc
     1201 gaagaccgtc tgaaagtgca cggcacaggc ggacgcttcc ccaaccgcac tgccgatttg
     1261 tttttgggca acgcgggcac ggcgttccgc ccgttaaccg ccgctctggc cgttttgggc
     1321 ggcgattatc atctgcacgg cgtgcctcgt atgcacgaac gtcctatcgg cgatttggtc
     1381 gatgcgttgc ggattgccgg ggccgatgtc gaatatctcg gcaaggaaca ctatccgccg
     1441 cttcatatcg gcgaacgcca agacaacggc gagcgcgtga ttccgattaa aggcaatgtg
     1501 tccagccagt ttctgaccgc ccttttaatg gcgttgccgc tgaccgggca ggcgtttgaa
     1561 atccgtatgg tcggcgaatt gatttccaag ccctatatcg acattacttt aaaactgatg
     1621 gcgcaattcg gcgtacaggt tatcaatgaa ggctaccgcg tcttcaaaat tcccgccgat
     1681 gcgcactacc acgcgcccga acacttgcac gtcgaaggcg atgcctccag cgcgtcctac
     1741 ttcctcgcag ccggtttgat tgccgccacg cccgtccgcg ttaccggtat cggcgcaaac
     1801 agcatacagg gcgatgtcgc ctttgcccgc gagctggaaa aaatcggggc ggacgtggtt
     1861 tggggcgaaa acttcgtcga agtttcacgc ccgaaggaac gtgccgtcca atcctttgat
     1921 ttggatgcga accatatccc cgatgccgcc atgaccctcg ccatcgtcgc gcttgctaca
     1981 aggcaaacct gcacgctgcg caacatcggt tcgtggcgcg tcaaagaaac cgaccgcatc
     2041 gccgcaatgg caaacgagtt gcgcaaactc ggggcaaaag tcgtcgaaga agccgaagca
     2101 attcacatca ccccgcccga aacgctgaca cccgacgccg tcatcgacac gtacgacgac
     2161 caccgcatgg cgatgtgttt ctcgctggtt tcgctgttgg gcgtacccgt cgtcatcaac
     2221 gatccgaaat gcacccacaa aaccttcccg acttatttcg acgtgttctc atcgctgacc
     2281 gaaacagcgg aataaggcgg cattttgccg cgattccggc gcggcgcggc gggcggctca
     2341 ttctgtaaaa aaagtatgtg cgccgaggta gtttttggcg taaaacggcg tggagagttt
     2401 ttcggttttg atggttttgc cgctgctggg ggcatggatg aattcgccgt tgccgatata
     2461 gagtccgacg tgtgagtagc ggtgtgcgcc gccggtgttg aagaatacga ggtcgccggc
     2521 cttgaggcgg ctgtcgggga ttttgcggct tgccgccgcc atgtctcggg cggtgcgcgg
     2581 cagcttgacg ttgagggcgt ttttgtaaac gaattggatc atgccgctgc aatcgaagcc
     2641 ggttgcggtg ctgctgccgc cccatttgta gggcgtgccg atgagtccga ggctgtggag
     2701 catgagttcc tgcgagcctt gtgtgcggtc gatgtggctg atgcggacgg cttggatttg
     2761 ccggactgtc tgtttgggtt tcggttggcg gtgtttgccg gaggtcgtgc cgcatgaggc
     2821 gaggagcagt gcgctgagac agaggaaaag ggttttgtcg gggggaaaca tggtttttcc
     2881 tttgcgggtt cggatatccg tctgaaggtg tttcagacgg tatagtggat taacaaaaat
     2941 caggacaagg cgacgaagcc gcagacagta ca
//
LOCUS       DAWHSK010000230         2948 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.230, whole genome shotgun sequence.
ACCESSION   DAWHSK010000230 DAWHSK010000000
VERSION     DAWHSK010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.230"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(295..732)
                     /gene="rnhA"
                     /locus_tag="WIC03_001840"
     CDS             complement(295..732)
                     /gene="rnhA"
                     /locus_tag="WIC03_001840"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEZ3884300.1"
                     /translation="MDKTVYLYTDGACKGNPGAGGWGVLMRYGSREKELFGGEAQTTN
                     NRMELTAVIEGLKSLKRRCTVIICTDSQYVKNGMENWIHGWKRNGWKTAAKQPVKNDD
                     LWKELDVLVGQHQVSWTWVKGHAGHAENERADDLANRGAAQFS"
     gene            846..1715
                     /gene="tehB"
                     /locus_tag="WIC03_001841"
     CDS             846..1715
                     /gene="tehB"
                     /locus_tag="WIC03_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HEZ3884301.1"
                     /translation="MKEWIVGQSGELFCFGQMPVWKAENLPEVLLSGYSSEGGEWVCL
                     NVLQGDVEVRTQDGAAEVWSAESGDCVFAPQQVFSVKPKTDDAEIRLSMYCVAEDYFH
                     KKYGMSATHSAVAAAQDIVPAGRALDMGCGQGRNALFLGLKGFEVTAVDHNPAALANV
                     AELAEAEGLNVRTLEYDLNAAALQGEFDYIVATVVLMFLMPQRVPDVIADMQAHTAAG
                     GYNLIVSAMDTADFPCPMPFPFKFKEGELKDYYRDWELVEYKEELGAMHAKDENGNPI
                     RFKFVTMLAKRPE"
     gene            complement(1765..2571)
                     /gene="thiD"
                     /locus_tag="WIC03_001842"
     CDS             complement(1765..2571)
                     /gene="thiD"
                     /locus_tag="WIC03_001842"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEZ3884302.1"
                     /translation="MKSSFVQTLTIAGSDSGGGAGIQADLKTFQMRGVFGTCVITAVT
                     AQNTLGVSAVHLVPTETITAQIQAIREDFDIRAYKIGMLGTAEIIECVADKLKHCDFG
                     RRVLDPVMIAKGGAPLLQDSAVAALTRLLLPDTDVLTPNLPEAEALTGVHIENRKDAE
                     RAAKILLDYGVKNVVIKGGHLNGSSSGRCTDWLFTQNETLELDSPRFPTAHTHGTGCT
                     FSACITAELAKGSDVCEAVQTAKAYITAAISNPLEIGAGHGPVNHWAYRD"
BASE COUNT          613 a          733 c          872 g          730 t
ORIGIN      
        1 gcagcgttgg atttttagat tcccgctttc gcgggaatga cggggcggtc ggatgccgcc
       61 tgaagcccga caaagccccc aatgccgtct gaaacctcgt cattcctctg ctgtccgcaa
      121 cttttcgtca ttcccgcaaa agcgggaatc tagagcctct aaattttcag acggcttttg
      181 aatattgccg ttgcccgacg gtttggattc ccgcctgcgc gggaatgacg gtggaggacg
      241 atgccgtcta aagcccatta gcggtttcag acggcatttt tgccggagcg gcagtcagga
      301 aaactgcgct gcgccacggt ttgccaaatc gtcggcgcgt tcgttttccg cgtgtcccgc
      361 gtgtccttta acccaagtcc agctgacttg gtgctgccca accagaacgt cgagttcttt
      421 ccacaagtcg tcgtttttga cgggctgttt ggcggcggtt ttccagccgt tgcgcttcca
      481 accgtgtatc cagttttcca tgccgttttt gacgtattgc gagtcggtgc agatgatgac
      541 ggtgcagcgg cgtttgagcg atttcagtcc ttcgatgacg gcggtcagct ccatgcggtt
      601 gttggtggtt tgcgcttcgc cgccgaaaag ttctttttcg cggctgccgt agcgcatcaa
      661 cacgccccag ccgcccgcgc cgggattgcc tttgcacgcg ccgtcggtgt aaaggtaaac
      721 ggttttgtcc attattgtgc ctttgtgtgg tgtccggcgt gattataagc ggtttgtgcg
      781 ccgtgccgtc tgaaagattg cggcggatta aaataggcat ataatgtgca actttatttt
      841 tgaatatgaa ggagtggata gtgggtcaaa gcggcgagtt gttttgtttc ggacagatgc
      901 ccgtatggaa ggcggaaaac ctgccggaag ttttgttgtc gggctattcg tccgaaggag
      961 gggaatgggt ctgcctgaat gtgttgcagg gcgatgtcga agtccgcacg caggacgggg
     1021 cggcggaggt ttggtcggca gaaagcggcg attgcgtgtt tgcgccgcag caggtgtttt
     1081 cggtcaaacc gaaaacggac gatgccgaaa tccgtttgtc gatgtattgt gtggccgaag
     1141 attatttcca caaaaaatac ggcatgagtg ccacgcattc tgcagtggcg gcggctcagg
     1201 atatcgtacc tgcgggcagg gcgctcgata tgggctgcgg gcaggggcgc aacgcgctgt
     1261 ttctcggttt gaaggggttt gaagttactg cagtcgatca caatcccgcc gccttggcaa
     1321 acgtggcgga gctggcagag gcggagggtt tgaacgtccg cacgctggaa tatgatttga
     1381 acgccgccgc cttgcagggc gagtttgatt atattgtggc aacagtggtg ctgatgttcc
     1441 tgatgccgca gcgcgtaccc gacgtgattg ccgatatgca ggcgcatacg gcggcgggcg
     1501 ggtacaactt gattgtatcg gcaatggata cggcggattt cccctgcccg atgccgttcc
     1561 ccttcaaatt taaagaggga gaactgaagg attattatcg ggattgggag ctggtcgaat
     1621 ataaagaaga attgggcgcg atgcacgcca aagacgaaaa cggcaatccg atacggttta
     1681 aatttgtaac catgctggcg aaaaggcctg aataatcttc aaaaatgccg tctgaacatt
     1741 ttgtttcaga cggcattttt acggttagtc ccgatacgcc caatgattga ccggcccgtg
     1801 tcccgcgccg atttccaaag ggtttgagat tgccgccgtg atataggcct tggcagtctg
     1861 tacggcttcg caaacgtccg agccttttgc caattcagcg gtaatgcagg cggaaaacgt
     1921 gcagcctgtg ccgtgcgtgt gggcggtcgg aaagcgcggg ctgtccaatt ccagcgtttc
     1981 gttttgcgta aacagccaat ccgtgcagcg tccgctgctg ctgccgttca aatgtccgcc
     2041 tttgataacg acatttttga caccgtaatc aagcaggatt tttgccgcac gttccgcatc
     2101 tttacggttt tcgatatgca cgccggtcag ggcttccgcc tcgggcaggt tgggggtcaa
     2161 tacatccgta tcgggaagca gcaggcgcgt cagtgccgca acggcggaat cctgcaacag
     2221 cggcgcaccg cctttggcaa tcatcacagg gtcgagtacg cgcctgccga agtcacagtg
     2281 tttcagcttg tcggcaacgc attcgatgat ttccgccgtg ccgagcattc ctattttgta
     2341 ggcgcggatg tcaaagtctt ccctgattgc ttggatttgt gcggtgatgg tttcggtcgg
     2401 gacgagatga accgccgaca cgcccaaggt attttgcgcg gtaacggcgg tgatgacgca
     2461 cgttccgaac acgccgcgca tctgaaacgt tttcaaatcc gcctgaatgc ccgcaccgcc
     2521 gcccgaatcc gaaccggcga tggtaagcgt ttgcacaaaa gagcttttca tttgttcctc
     2581 cttaatattg tgtccgataa aaagggctaa tgcctgtaaa aaagcacggc gatgccgtct
     2641 gaaaaccgca gcgcggggtt tgtggtgcat tatatgccgt attgcaggac aaggcacagg
     2701 gcagggcggc ggggtgcatc gtagcaactg tatttttgcc cgacggggtg aaaaatacag
     2761 ttgctacacc cactgataat gaaaaaagag agcaatgccg tctgaaccgc gtgttgcggc
     2821 gagcataaac cggcacgctt tattcgtcat tcccacaaaa acagaaacct aaaattccgt
     2881 cattcctacg aaagcgggaa tccagtgtgt tcggtttcag ttgtttttgg gtttcgggtg
     2941 atttccaa
//
LOCUS       DAWHSK010000231         2935 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.231, whole genome shotgun sequence.
ACCESSION   DAWHSK010000231 DAWHSK010000000
VERSION     DAWHSK010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.231"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..1503
                     /locus_tag="WIC03_001843"
     CDS             <1..1503
                     /locus_tag="WIC03_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEZ3884303.1"
                     /translation="LGGILAGGGTSLAAPYLDKAAENLGTAGKAAVNALGGAAIGYAA
                     GGNVGTAAVGANVDWNNRQLHNSEIRKIKNNAAKFAKLHPGISQRQAEERLVAQAMRQ
                     VDAAYYAKHADTDTAAEQFLRSIAGSFKADGETFLDFAPMKYYRDSGKFAEHTGYRDL
                     RAKLGQNPVHTEAQRNRTIEEKTYGTIGRGVGKGIGNTLANNLQAGPPHYVLGITNYS
                     NWGKELIPYENPVERAIGKQAEDMTEKAVAAVSLGRSLPSKVVNVPSKLAKSIKTAET
                     HKPLVQKRTETYFRVEGGGSGNNQAAKAAKNTEKMKAAIDQTHSGVDLPYDSRQIRAK
                     LEKRYGAENIVSTTIPKKPVQSINNQTKPDYSSIVYTNKSGKVVNVKYKDPISGESKA
                     VNIAYDNRGLPIFDNVSNFTTKIDNSLSYTGQMRAATKDLWKAIESGQVPKNQFTNEQ
                     LTKIKAGNEKIPGYTWHHNAQSSPNNMQLIPNSIHTAVRHTGQKSLKKGQ"
     gene            1506..1982
                     /locus_tag="WIC03_001844"
     CDS             1506..1982
                     /locus_tag="WIC03_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEZ3884304.1"
                     /translation="MRKLSISSDEGLVPIRTIQQFAEDVGVVFPASYVSLLSKHDHLY
                     PKENIFNFVNQYGENDERDISFLGYKQDLGYEDIYSYSCIDDEYGYAKKVIAFGISAN
                     GDYICFDYRKCNENPCIILMYHDDFYEDENGDTKMVTSHIADSFDAFLDMLYELSD"
     gene            2096..>2935
                     /locus_tag="WIC03_001845"
     CDS             2096..>2935
                     /locus_tag="WIC03_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HEZ3884305.1"
                     /translation="MRFSRFEPKGRLKQQIRFQTTFLSTSHHGNQTKAAHRHIHVGST
                     AGTTTIRSGGDTTLKGAQLIGKGIQADTRNLHIESVQDTETYQSKQQNASAQVTVGYG
                     FSASGDYSQSKIRADHASVTEQSGIYAGEDGYQIKVGNHTDLKGGIITSSQSAEDKGK
                     NRFQTATLTASDIQNHSQYKGESFGLGASASISGKTLGQGAQNKPQNKHLTSVADKNG
                     ASSSVGYGSDSDSQSSITKSGINTRNIHITDEAGQLARTGRTAKETEARIYTGIDTET
                     ADQH"
BASE COUNT          994 a          634 c          692 g          615 t
ORIGIN      
        1 ctcggcggca tactggccgg cggcggcact tcccttgccg caccgtattt ggacaaagcg
       61 gcggaaaacc tcggtacggc gggcaaagcg gcggtcaacg cactgggcgg ggcggcaatc
      121 ggctatgcgg cgggcgggaa tgtcggcacg gcggcagtag gggcgaatgt cgattggaac
      181 aataggcagc tgcacaacag cgagatcaga aaaattaaaa ataatgccgc taaatttgcc
      241 aaactccatc ccggcatttc ccaacggcag gcagaagagc ggctggtggc acaagctatg
      301 cgtcaggtcg atgcggctta ttatgccaaa catgccgaca ccgacacggc ggcggaacaa
      361 ttcctgcgca gcatagcggg aagtttcaaa gccgacgggg aaacgttcct ggattttgcc
      421 ccgatgaagt attacaggga cagcggtaaa tttgccgaac atacaggcta tcgagatcta
      481 agagcaaagt tgggacagaa tccggtccat accgaagcgc aaaggaacag aactattgaa
      541 gaaaagacct acggcacaat aggcagaggt gtaggtaaag gcatcggcaa taccctagcc
      601 aacaacctgc aggcgggtcc gccacactat gtactcggca ttacgaacta ttcgaactgg
      661 ggcaaagaac ttattccata cgagaaccct gttgagcgcg ccatcggcaa acaggcagag
      721 gatatgacgg aaaaagcggt agcagctgtg tctttgggcc ggagtttgcc gtcaaaggtt
      781 gttaatgtac cctctaaatt agctaaaagc attaagacag cggaaacaca taaaccactg
      841 gtacaaaagc gtacggaaac ttattttaga gttgaaggag gaggaagtgg aaataatcag
      901 gcagccaaag ccgctaaaaa tacggaaaaa atgaaagccg caatagatca aacccacagt
      961 ggagttgacc tgccttatga ttcgagacaa attcgagcta aactggaaaa aagatatgga
     1021 gctgagaata ttgtatccac tacgattcca aagaaaccgg tccaaagtat caataatcaa
     1081 actaaaccgg actactcatc tattgtatat actaataaaa gtggaaaggt agttaatgtt
     1141 aaatataaag atcctatatc aggggaaagt aaggctgtta atatcgccta tgataatagg
     1201 ggattaccta tatttgataa cgtatcaaat tttaccacca aaatagataa ttcattgtcg
     1261 tatacgggac agatgagggc agctactaaa gatttatgga aagctattga aagtggacaa
     1321 gtgcccaaaa atcaatttac taatgaacaa ctaacgaaga ttaaagcagg aaatgaaaaa
     1381 attcctggtt atacatggca tcataatgcg caaagctctc ctaataatat gcagttaatt
     1441 cctaatagta ttcatacagc tgttaggcat actggtcaaa aatcgttaaa gaaaggtcag
     1501 taaaaatgag aaaactatca atatcatcag atgagggatt ggttccaata agaacaattc
     1561 aacaatttgc tgaagatgta ggggtcgttt ttcccgcgtc atatgtatca cttcttagta
     1621 aacatgatca tttataccct aaagaaaata ttttcaattt tgtcaatcaa tacggtgaaa
     1681 atgatgaaag ggacatttca tttttagggt ataagcaaga tttaggctat gaagatattt
     1741 atagctattc atgtattgat gatgaatatg ggtatgcaaa aaaagtgatt gcttttggga
     1801 tttcagccaa tggcgattat atctgttttg attacagaaa atgcaatgaa aatccatgta
     1861 tcattctgat gtatcatgat gatttctatg aagatgagaa tggggatacg aaaatggtaa
     1921 ctagccatat tgcagatagt tttgatgcct ttttggatat gctatatgaa ctttcagatt
     1981 aagtgtgtag gttgagttga aaacccaact aaattaaaaa ggtcgtctga aatcagcaag
     2041 ccgtagccca tatgaaacct aaactcaaca agtaggatgt gtgcggaacg cacgcatgcg
     2101 gttctcaagg tttgagccca aaggtcgtct gaaacaacaa atacggtttc agacgacctt
     2161 tctttcaaca agccaccacg gcaatcagac aaaagcagca catcgccaca tccatgtcgg
     2221 cagtacggcc ggcacaacca ccatccgcag cggcggggat accaccctca aaggtgcgca
     2281 gctcatcggc aaaggcatac aggcagatac gcgcaacctg catatagaaa gtgttcaaga
     2341 tactgaaacc tatcaaagca aacaacaaaa cgccagtgca caagtgaccg taggttatgg
     2401 cttcagtgcc agtggcgatt acagccaaag caaaatccga gccgaccatg cttcggtaac
     2461 cgagcaaagc ggtatttatg ccggagaaga cggctatcaa atcaaggtcg gaaaccatac
     2521 agacctcaaa ggcggcatca tcaccagcag ccaaagcgca gaagacaagg gtaaaaaccg
     2581 ctttcagacg gccaccctta ctgccagcga cattcaaaac cacagccaat acaaaggcga
     2641 aagttttgga ttgggcgcaa gtgcgtccat aagcggcaaa acactgggac agggcgcaca
     2701 aaataagcct caaaacaaac acctgacaag cgtggccgat aaaaacggcg caagttcatc
     2761 agtgggttat ggcagcgaca gcgacagtca aagcagcatc acgaaaagcg gcatcaacac
     2821 ccgcaacata cacatcaccg acgaagcggg acaacttgcc cgaacaggca ggactgccaa
     2881 agaaaccgaa gcgcgtatct acaccggcat cgacaccgaa actgcggatc aacac
//
LOCUS       DAWHSK010000232         2922 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.232, whole genome shotgun sequence.
ACCESSION   DAWHSK010000232 DAWHSK010000000
VERSION     DAWHSK010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.232"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..1308
                     /locus_tag="WIC03_001846"
     CDS             <1..1308
                     /locus_tag="WIC03_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEZ3884306.1"
                     /translation="VGLLTEAFLYEEQTRRGVSVKHFEELADIGDHCTVCHRCVKPCP
                     VNIDFGDVTVAVRNYLADSGHKRFAPAASMGMAFLNATGPKTIKTLRAAMIQTGFPAQ
                     NFAYKIGKLLPVGTKKQKAEPKATVGKAPIKEQIIHFINRPLPKNVPAKTPRSLLGIE
                     DDKSIPIIRNPASPEDAEAVFYFPGCGSERLFSQIGLAVQAMLWHVGVQTVLPPGYMC
                     CGYPQDAGGNKAKAEEMSTNNRVAFHRMANTLNYLDIKTVVVSCGTCYDQLEKYRFEE
                     IFPGCRIIDIHEYLLEKGVKLNGVKGQQYLYHDPCHTPIKTMNATQMASSLMGQKVVL
                     SDRCCGESGMFAVKRPDIATQVKFRKQEEIEKNLKELPQGEPVKMLTSCPACLQGLSR
                     YADDNNMPTDYIVIEMAKHILGENWLDEFVKKANNGGVEKVLL"
     gene            1384..2385
                     /locus_tag="WIC03_001847"
     CDS             1384..2385
                     /locus_tag="WIC03_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEZ3884307.1"
                     /translation="MIDGQTTEPKQKTRIILAPMQGLVDDVMRDLLTRIGGYDECVSE
                     FVRITHTVHSRSIWLKYVPEIANGNKTFSGTPCTVQLLGSDADNMAANALEAVRFGAN
                     KIDLNFGCPAPTVNKHKGGAILLKEPELIFHIVKTLRGRLPAHIPLTAKMRLGYEDKS
                     RALECACAIAEGGACGLTVHARTKAEGYEPPAHWEWIRKIRDSVNIPVTANGDVFSLQ
                     DYIGIKTISGCNSVMLGRGAVIRPDLARQIKQYENGGPVKDTDFAEVSKWIRQFFELC
                     LTKEANNKYPIARLKQWLGMMKKTFDPAQTLFDHVRTVKDADEVRRILNAFEHEINV"
     gene            complement(2447..2629)
                     /locus_tag="WIC03_001848"
     CDS             complement(2447..2629)
                     /locus_tag="WIC03_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884308.1"
                     /translation="MFWYVIGFCSFVVALLSLWVNVGAFGMQEDDTPQSDYEKRLGLG
                     AKLKNKNTPDTEEKQQ"
     gene            2816..>2922
                     /locus_tag="WIC03_001849"
     CDS             2816..>2922
                     /locus_tag="WIC03_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEZ3884309.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDQVIDWQPIE"
BASE COUNT          816 a          860 c          676 g          570 t
ORIGIN      
        1 gtggggctct tgaccgaagc ctttttatac gaagaacaaa cccgtcgcgg cgtttccgtc
       61 aaacattttg aagaactggc agacatcggc gaccactgca ccgtgtgcca ccgctgcgtc
      121 aaaccctgcc ccgtcaatat cgacttcggc gacgtaaccg tagccgtccg caactacctt
      181 gccgattccg gccacaaacg ctttgcgccg gcagcttcaa tgggtatggc attcctaaac
      241 gcgaccggcc cgaaaaccat taaaacccta cgtgccgcga tgatacaaac cggcttcccc
      301 gcgcagaatt tcgcctacaa aatcggcaaa ctgctgcccg tcggcacgaa aaagcaaaaa
      361 gccgaaccca aggcaaccgt cggcaaagcc ccgattaaag aacaaattat ccacttcatc
      421 aaccgtccgc tgcccaaaaa cgtgcctgcc aaaacaccgc gctccttatt gggcatcgaa
      481 gacgacaaaa gcatccccat catccgcaat ccggcttctc cggaagatgc cgaagccgtg
      541 ttttactttc ccggctgtgg ttcggaacgc ctgttcagcc aaatcggact cgccgtccaa
      601 gccatgctct ggcacgtcgg cgtgcaaacc gtcctgccgc ccggctatat gtgttgcggc
      661 tatccgcagg acgccggcgg caataaggca aaagccgaag aaatgagcac caacaaccgc
      721 gtggctttcc accgtatggc aaacaccctc aactacctcg acatcaaaac cgtcgtcgtc
      781 agttgcggta cctgctacga ccagctcgaa aaataccgct tcgaagaaat cttccccggc
      841 tgccgcatca tcgacatcca cgaatacctg ctcgaaaaag gcgtgaaact gaacggcgtg
      901 aaaggtcagc aatacctcta ccacgacccc tgccacacgc ccatcaaaac catgaacgcc
      961 acccaaatgg ccagcagcct gatggggcag aaagtcgtct taagcgaccg ctgctgcggc
     1021 gaatccggca tgtttgccgt caaacgcccc gacatcgcca cccaagtcaa attccgcaaa
     1081 caagaggaaa ttgagaaaaa cctcaaagag ctgccgcagg gcgaacccgt caaaatgctg
     1141 acctcctgtc cggcgtgtct gcaaggcttg agccgctacg ccgacgataa caatatgccc
     1201 accgactaca tcgttatcga aatggcgaaa cacatcctcg gcgaaaactg gctagacgag
     1261 tttgtgaaaa aagccaataa cggcggcgtg gaaaaagtat tgctgtaaca acggacacgg
     1321 aaatgccgtc tgaacgccga agtcttcaga cggcattgtt taaacccaat acctacccaa
     1381 atcatgattg acggacaaac aaccgaaccc aaacaaaaaa cccgaatcat ccttgcccct
     1441 atgcagggtc tggtcgatga cgtgatgcgc gacctgctga cgcgtattgg cggctacgac
     1501 gaatgcgtca gcgaatttgt acgcattacc cataccgtgc attcccgatc catatggtta
     1561 aaatatgtcc ccgaaatcgc caacggaaac aaaacgtttt ccggcacgcc ctgcaccgtc
     1621 caacttttgg gcagcgatgc ggacaatatg gcggcgaatg cgctggaagc cgtccgcttc
     1681 ggtgcgaaca aaatcgattt gaacttcggc tgccccgcgc ccaccgtcaa caaacacaaa
     1741 ggcggcgcaa tccttttaaa agagccggaa ctgatattcc acatcgtcaa aacgctgcgc
     1801 ggacgtttgc ccgcacatat tccgctcacc gcaaaaatgc ggctcggtta cgaagacaaa
     1861 agccgggctt tggaatgcgc ctgtgcgatt gccgaagggg gcgcatgcgg actgaccgta
     1921 cacgcgcgta ccaaagccga gggttacgaa ccgccggcgc attgggaatg gataaggaaa
     1981 atccgagaca gcgtcaatat tcccgttacc gccaacggcg acgttttcag cctgcaagac
     2041 tatatcggca tcaaaacaat cagcggctgc aacagcgtga tgctcggtcg cggcgcggtc
     2101 atccgccccg atttggcgcg gcaaatcaag caatacgaga acggcgggcc ggtcaaagac
     2161 acggattttg ccgaagtttc caaatggata cggcagtttt tcgagctgtg cctgacaaaa
     2221 gaggcaaaca ataaataccc gatcgcccgt cttaagcaat ggttgggcat gatgaaaaaa
     2281 acgttcgacc cggcgcaaac gctgtttgac cacgtccgaa cggttaagga tgcggatgaa
     2341 gtccgccgaa tcctgaacgc atttgagcat gagataaatg tttgactgtc tcccatgctt
     2401 caaagcaaat gccgtctgaa aatctttcag acggcatttc atatctttat tgctgctttt
     2461 cttccgtatc cggcgtattt ttgtttttca gcttcgcacc caagcccaaa cgtttttcat
     2521 aatccgattg cggggtatcg tcttcctgca taccgaatgc gccgacattg acccacagcg
     2581 acagcagcgc gacgacaaag gagcaaaagc cgattacata ccaaaacatt gcccctcccg
     2641 atttgttaaa atcatatcaa atacagtgcc gaatttatca caaacgcacg ggcaaatata
     2701 gtgaattaaa tttaaataag gacaaggcgg cgagccgaag acagtacaaa tagagacctt
     2761 tgcaaaattc cccaaaatcc cctaaattcc caccaagaca tttaggggat gttccatgag
     2821 caccttcttt cagcaaaccg cacaagccat gattgccaaa cacatcgacc gtttcccact
     2881 attgaagttg gatcaagtga ttgattggca gccgatcgaa ca
//
LOCUS       DAWHSK010000233         2906 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.233, whole genome shotgun sequence.
ACCESSION   DAWHSK010000233 DAWHSK010000000
VERSION     DAWHSK010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2906
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.233"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..857
                     /gene="tgt"
                     /locus_tag="WIC03_001850"
     CDS             <1..857
                     /gene="tgt"
                     /locus_tag="WIC03_001850"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEZ3884310.1"
                     /translation="LTDSGGFQVFSLSDMRKLTEEGCTFKSPINGDKLFLSPEISMKI
                     QTVLNSDIAMQLDECTPGEATREQARKSLQMSLRWAERSKKAFEDLKNPNALFGIVQG
                     AMYEDLREESLKGLEELDFPGLAIGGLSVGEPKPEMYRMLCAVGPILPEHKPHYLMGV
                     GTPEDLVYGVAHGVDMFDCVMPTRNARNGWLFTRFGDLKIKNAKHKLDKRPIDESCTC
                     YACQNFSRAYLHHLHRAGEILGAQLNTIHNLHFYQVIMAEMRDAIEQGKFADWQARFH
                     ENRARGTD"
     gene            867..1949
                     /locus_tag="WIC03_001851"
     CDS             867..1949
                     /locus_tag="WIC03_001851"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEZ3884311.1"
                     /translation="MPSEAASDGILPLYPTRTIFQKPIMLPFLPEPSLSYTQQNRTAV
                     LLLNLGTPDAPTAQAVRPYLKSFLTDRRVVELPKWLWYPILHGLVLTLRPKKSAHAYE
                     KIWFKEGSPLEVYTARQAAALAERMPDLIVRHAMTYGNPSVADVLSELKAQGAGRLLV
                     IPMYPQYAASSSGAAVDKVCEQLLLQRNQMSVRTVSRFYDDTGYIDAMKNHILRYWAE
                     HGRGKKLMLSFHGVPQKHYDLGDPYPDECRHTAKLLAEALELTEDQYVVSFQSQFGRA
                     KWVTPSTQDLFGKLPKQGVTELDVFCPGFLADCLETMEEIALMGREQFYEAGGKSYRY
                     IPCLNDNPDWIDALVALAEENLGGWR"
     gene            2141..2542
                     /locus_tag="WIC03_001852"
     CDS             2141..2542
                     /locus_tag="WIC03_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEZ3884312.1"
                     /translation="MNTTRLPTALVLGCLCAAASAADNSIMTKGQKVYESNCVACHGK
                     KGEGRGTMFPPLYRSDFIMKKPQVLLHSMVKGINGTIKVNGKTYNGFMPATAISDADI
                     AAVATYIMNAFDNGGGSVTEKDVKQAKGKKN"
     gene            complement(2605..2895)
                     /locus_tag="WIC03_001853"
     CDS             complement(2605..2895)
                     /locus_tag="WIC03_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884313.1"
                     /translation="MAKYFTETGIGVREHFDFFGEFVVSPAARSGDLALAYGLRLEAG
                     EEGLSLAELFDKRSDSQEPVECGRIDIGGFMLTAKEVDGGGNIGSMGLKVPR"
BASE COUNT          760 a          861 c          709 g          576 t
ORIGIN      
        1 ttttgaccga ctcgggcggt tttcaggttt tttctttgtc ggatatgcgc aagctgacgg
       61 aagaaggctg tacgtttaaa agcccgatta acggcgacaa gctgttttta tcgcctgaaa
      121 tttctatgaa aatccaaacg gttttgaact cggacatcgc gatgcagttg gacgaatgca
      181 cgccgggcga ggcgacgcgc gaacaggcgc gaaaatcgtt gcaaatgagc ctgcgctggg
      241 cggaacggag caaaaaagcc tttgaagatt tgaaaaaccc gaacgcactg ttcggcatcg
      301 tgcaaggcgc gatgtatgaa gatttgcgcg aagagtcgct gaaaggcttg gaagagctcg
      361 atttccccgg cttggccatc ggcggcttgt ccgttggcga acctaagccc gaaatgtacc
      421 gcatgttgtg cgccgtcggc cctatcctgc cggaacacaa acctcattat ttaatgggcg
      481 tcggtacgcc tgaagactta gtgtacggcg tggcacacgg cgtggatatg ttcgactgcg
      541 tcatgcctac tcgcaatgcg cgtaacggct ggctgtttac ccgttttggc gatttgaaga
      601 tcaaaaacgc caaacacaag ctcgacaagc gtccgataga cgaaagctgc acctgctacg
      661 cctgccaaaa cttcagccgc gcctacctgc accatctgca ccgcgccggc gaaatcttgg
      721 gcgcacagct caacaccatc cacaacctgc atttctacca agtcattatg gcggaaatgc
      781 gcgatgccat agaacaaggc aaatttgccg actggcaggc gcgattccac gaaaaccgtg
      841 ctcgcggcac agactgactc ccggcaatgc cgtctgaagc cgcttcagac ggcattttgc
      901 cgctttaccc tacccgcact atttttcaga aaccgatcat gcttccattc cttccagaac
      961 cctccctttc ctatacgcaa caaaaccgta ccgccgtttt gctgttgaac ctcggcacgc
     1021 ccgacgcgcc gaccgcgcag gcggtacgac cttatctgaa gtcctttctg accgaccggc
     1081 gcgtcgtcga actgcccaaa tggctgtggt atcccattct gcatggtctg gtactgacgt
     1141 tgcgccccaa aaaaagcgcg cacgcctatg aaaaaatctg gtttaaggaa ggttcgcccc
     1201 tagaagttta cacagcacgc caagctgccg cgcttgccga acgtatgccc gacctcatcg
     1261 tccgccacgc catgacctac ggcaatcctt cggtggcaga cgtattgtcg gaactgaagg
     1321 cgcaaggcgc gggcagactg ctggtcatcc cgatgtaccc gcaatatgcc gcttccagca
     1381 gcggagcggc agtagataag gtgtgcgaac aattactgct gcaacggaac caaatgagcg
     1441 tccgcaccgt ttcgcgcttt tatgacgata ccggctacat tgatgcgatg aaaaaccaca
     1501 tcctccgata ttgggcggaa cacggacgtg ggaaaaaact gatgttgagt tttcacggcg
     1561 taccgcagaa gcactacgac ctcggcgacc cctatcccga cgagtgccgc cacaccgcca
     1621 aactgcttgc cgaagcactg gaactgaccg aagaccaata tgtcgtatcg ttccaaagcc
     1681 aattcggcag ggcaaaatgg gttacaccga gcacgcagga tttgttcggc aaactgccca
     1741 aacagggggt aaccgagctg gacgtattct gcccgggctt tttggcagac tgtttggaaa
     1801 ccatggaaga aatcgccctg atggggcggg aacagtttta cgaagcaggc ggcaaaagct
     1861 accgctacat cccctgcctc aacgacaacc ccgactggat agacgcactc gtcgcacttg
     1921 ccgaagaaaa ccttggcggc tggcgttgaa actgtttgac ccatgtctaa aatgccgtct
     1981 gaacgccgtg ttcagacggc attttcccgc tactttagcg tatgatggca tctatgccgg
     2041 acaacaacat caagtgcaac cttactaccg attcggcaaa aaaatctttc acccgctacg
     2101 caaaatcaac aacaaggttt caaacattaa ggaaacacca atgaacacaa cccgactgcc
     2161 gaccgccctc gtcttgggct gcctctgcgc cgccgcttct gccgccgaca acagcatcat
     2221 gacaaaaggg caaaaagtgt acgaatccaa ctgcgtcgcc tgccacggca aaaagggcga
     2281 aggccgcgga accatgtttc cgccgctcta ccgctccgac ttcatcatga aaaaaccgca
     2341 ggtgctgctg cacagcatgg tcaaaggcat caacggtaca atcaaagtca acggcaaaac
     2401 ctacaacgga ttcatgcccg caaccgccat cagcgatgcg gacattgccg ccgtcgccac
     2461 ttatatcatg aacgcctttg acaacggcgg cggaagcgtt accgaaaaag acgtaaaaca
     2521 ggcaaaaggc aaaaaaaact aaacagacaa aatgccgtct gaaaccggca atccggcttc
     2581 agacggcatt caaatcaaac ctttctaacg cggtactttc agccccatag acccgatatt
     2641 gccgccaccg tcaacctcct tcgcggtcag cataaagccg ccgatgtcaa tacggccgca
     2701 ctcgaccggc tcctgactgt cggaacgctt atcgaaaagc tcggcaaggc tcaaaccctc
     2761 ttcgcccgct tccagcctca aaccgtaagc aagtgccaaa tcacccgaac gtgctgccgg
     2821 cgaaacgaca aactcgccga aaaaatcaaa atgctcacgg acaccgatac ccgtctcggt
     2881 aaagtatttc gccattttat agtgaa
//
LOCUS       DAWHSK010000234         2896 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.234, whole genome shotgun sequence.
ACCESSION   DAWHSK010000234 DAWHSK010000000
VERSION     DAWHSK010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.234"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(120..1034)
                     /locus_tag="WIC03_001854"
     CDS             complement(120..1034)
                     /locus_tag="WIC03_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEZ3884314.1"
                     /translation="MDAVQLKSFVAVAHEGNLTQAAKRLFLSQPAVSAQIKALEEYVG
                     TPLFRRTGKGMVLTRAGEILLPEAESLLQYKHKLEHFAKTLAGDYSEETSLGIIHPID
                     SAKLVALTDNIGQTAPKTRLHIQYGMSGEILSRIQHKTLHGGFILGNAAQRGIRSVFL
                     QNLTYALICPQSQYPHLTRSLPQSLQECVWIEMSGVSGSRKHLHQFWRSNRLSPKKQI
                     LCDYPQTIIDLVAGGIGVAMVPGNKAEAAAKEGAGVAIIESCRHSMPLNFIYAEEYED
                     NPHVSLLLECIEKVWGVQAVQPPVVSDN"
     gene            1290..2759
                     /locus_tag="WIC03_001855"
     CDS             1290..2759
                     /locus_tag="WIC03_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3360 family protein"
                     /protein_id="HEZ3884315.1"
                     /translation="MEKHNGTYRDLHRPASEFATRDEYLEHELQIMQPKRWRPNLPFR
                     DYRFEWEDLIPAMAGTIGKVVMVGAVAAAFAAPLGLPDSFVLENVRYELLIAAAFILL
                     VSGFFLPGANLPGTHGPLIPMIPIVVSAGGHPLAFGISIAVLGLLMALFRGGSIMAKL
                     TSNGVCGGLLLYLGFIGTTGQVKKLFSWAGGFNMPYIAFTVIIVTIVMYALLEHWKKR
                     WLAVPLGCLIAGVVAFALGAPFEFHTAPGLPPMSPAYWWGENSGWHLGLPTAESFLVV
                     FPFAVLAVAMWSPDFLGHQVFQKISYPEKTDKVLMNIDDTMTSCSVRQAVGSILGGAN
                     FTSSWGTYIVPASIAKRPIPGGAVLTAVLCIIAGLWGYPMDLAIWQPVLSVALVVGVY
                     LPLLEAGMEMTRKGKTTQSAAIVVFSSALVNPVFGWALTMLLDNLGLIGCKERSAQLG
                     FAGRVLIPAVGFLILCVAMSAVGMLPGIPPFLEHFKSLG"
BASE COUNT          600 a          644 c          827 g          825 t
ORIGIN      
        1 ggcatttcca aagggcattt taagccgtat ttgagagaat gcggatggcg ttttcagtgt
       61 tttcagcgtg ataaaattca aatttctatt ttaaaacaat cagcaaagca ggatttattt
      121 cagttgtccg agacaacggg cggctgcacc gcctgcactc cccatacttt ttcaatgcac
      181 tcgagcagga gtgagacgtg gggattatcc tcgtattctt ccgcataaat gaaattgagc
      241 ggcatactgt ggcggcacga ttcgataata gccacgcccg cgccttcttt tgccgccgct
      301 tcggctttgt ttcccggcac cattgccaca cctataccgc ctgcaaccaa atcgataatg
      361 gtttgggggt agtcgcacaa gatctgtttt ttgggtgaga gccggttgct gcgccaaaac
      421 tggtgcaggt gcttcctact tccggacacg cccgacattt ctatccatac gcattcttgc
      481 aggctctgcg gaagggagcg ggtcagatgg ggatattggc tttgcgggca aatcagcgcg
      541 taggtcaggt tttgcaggaa tacgctgcgg atgccgcgtt gggcggcgtt gccgagtata
      601 aagccgccgt gcagggtttt gtgttggatg cgcgagagga tttcgccgct cattccgtat
      661 tggatgtgca ggcgcgtttt gggggctgtt tgaccgatat tgtccgtcag cgcgacgagt
      721 tttgccgaat cgatggggtg gataatgccc aaactggtct cttccgaata atcgcctgcc
      781 agcgttttgg caaaatgctc cagcttgtgt ttgtattgca gcagggattc cgcttcgggc
      841 aacagtattt cgcccgcccg cgtcaatacc atgcctttcc ccgtgcgcct gaacagcggc
      901 gtgccgacat attcttcaag ggctttaatt tgggcggaaa cggcaggctg ggaaaggaaa
      961 agtcgtttgg cggcttgggt aaggttgccc tcgtgcgcga cggcgacaaa tgattttaat
     1021 tgtacggcat ccatatatcc ctccttgtgc ggatgttttc tatatttgtg caatcgaaat
     1081 cttttaggtg gattgttgct gaaaattaac tttttaatca agtggtttgt aaattgtatc
     1141 agttttccgg atgatggtta ttaaaaaaaa agattggttt tattgcctgt ttgggcttta
     1201 aatggggtta cggcttccga acgcagcccg tatcaaaaag aaaagtcatg cgcccctttt
     1261 acgaggcgcg atatataagg aggaaggtta tggaaaaaca taatgggact tatcgggatt
     1321 tgcaccgtcc ggcttcggaa tttgcgacgc gggacgaata tttggaacat gaattgcaga
     1381 ttatgcaacc aaaacgctgg cggcccaacc tgccctttcg cgattaccgc ttcgagtggg
     1441 aggatttgat tcctgcgatg gcgggaacga tcggaaaagt ggtgatggtg ggggcggtgg
     1501 cggcggcgtt tgccgcacct ttggggctgc ctgacagctt tgtactggaa aatgtgcgct
     1561 atgagctttt aatcgccgcc gcgtttatct tattggtatc gggctttttt ctgcccggcg
     1621 ccaacctgcc cggtacgcac gggccgctga ttccgatgat tcccatcgtt gtgtcggcag
     1681 gcgggcatcc tttggcgttc ggcatttcga ttgcggtttt aggtctgctg atggctttat
     1741 ttcgcggcgg cagtattatg gcgaagctga caagcaacgg cgtatgcggc ggattattac
     1801 tctatttggg ctttatcggc acgacggggc aggtaaaaaa attgttttcg tgggcaggcg
     1861 gttttaatat gccctacatc gcttttaccg tcattattgt aacgattgtg atgtacgctt
     1921 tgttggagca ttggaaaaaa cgctggttag ccgtgccttt gggatgcttg attgccggtg
     1981 tggtggcatt tgcattgggt gcgccgtttg agtttcacac cgcccccggc ctgcctccaa
     2041 tgagtcctgc ttattggtgg ggtgaaaaca gcggctggca tctggggttg ccgacggcag
     2101 aaagtttttt ggttgtcttt ccatttgcgg tattggctgt tgcaatgtgg tcgcccgatt
     2161 ttttaggaca tcaagtgttc caaaaaatca gctatccgga aaaaaccgat aaggtattga
     2221 tgaatataga cgacaccatg acaagttgtt ctgtccgtca agcagtgggt tctattttag
     2281 ggggtgcaaa ttttacctct tcttggggaa cttatatcgt accggcatcg attgccaaac
     2341 gccccattcc gggcggtgcg gttttaacgg cggttttatg tattatcgcc gggttatggg
     2401 gctatccgat ggacttggcg atttggcagc cggtattgag cgtagccttg gtcgtaggcg
     2461 tatacttacc gcttttggaa gcgggcatgg aaatgacgcg caaaggcaaa accacccaat
     2521 ccgccgccat cgtggtgttc tcttccgcct tggtcaatcc ggttttcggc tgggcgttga
     2581 cgatgctgtt ggataatttg ggcttaatcg gctgcaaaga gcgcagtgcg caattaggtt
     2641 tcgccggacg cgtgttgata cccgcagtag gtttcttgat cttgtgtgtg gcgatgagtg
     2701 cggtcgggat gctgcccggt atcccgccgt ttttggaaca cttcaaatct ttgggctagg
     2761 ctgaaatcgg aaatgccgtc tgaaccgctt tcagacggca tttttgcaaa caggcaaaat
     2821 gacggcggcg ggatttttta ttttcccgat tgaagtataa tgttgccggg cttcaaccgg
     2881 atattcaaaa cggttt
//
LOCUS       DAWHSK010000235         2889 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.235, whole genome shotgun sequence.
ACCESSION   DAWHSK010000235 DAWHSK010000000
VERSION     DAWHSK010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.235"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(214..1278)
                     /locus_tag="WIC03_001856"
     CDS             complement(214..1278)
                     /locus_tag="WIC03_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEZ3884316.1"
                     /translation="MEKEFRILNIVSAKIWGGGEQYVYDVSKALGLRGCTMFTAVNKN
                     NELMHRRFSEVSSVFTTRLHTLNGLFSLCALTRFIRKNRISHLMIHTGKIAALSILLK
                     KLTGVRLIFVKHNVVANKTDFYHRLIQKNTDRFICVSRLVYDVQTADNPFKEKYRIVH
                     NGIDTGRFPPSQEKPDSRFFTVAYAGRISPEKGLENLIEACVILHRKYPQIRLKLAGH
                     GHPDYMCRLKRDVSASGAEPFVSFEGFTEDIASFYRQSDVVVLPSLVPEAFGLSLCEA
                     MYCRTAVISNTLGAQKEIIEHHQSGILLDRLTPESLADEIERLVLNPEAKNALATAGH
                     QCVANRFTINHIADKLLDAI"
     gene            complement(1279..2037)
                     /locus_tag="WIC03_001857"
     CDS             complement(1279..2037)
                     /locus_tag="WIC03_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEZ3884317.1"
                     /translation="MKKVSVLIVARNEANHIRECIESCRFDKEVIVIDDHSTDNTAEI
                     AEGLGAKVFQRHLNGDFGAQKTFAIEQAGGEWVFLIDADERCTPELSDEISKIVQTGD
                     YAAYFVERRNLFPNHPATHGAMRPDSVCRLMPKKGSSVQGKVHETVQTPYPKRRLKHF
                     MYHYTYDNWEQYFNKFNKYTSISAEKYREQGKPVRFVRDIILRPIWGFFKIYILNKGF
                     LDGKMGWIMSVNHSYYTMIKYVKLYYLYKSGGKF"
     gene            complement(2112..2187)
                     /locus_tag="WIC03_001858"
     tRNA            complement(2112..2187)
                     /locus_tag="WIC03_001858"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2152..2154),aa:Ile,seq:cat)
     gene            complement(2241..>2889)
                     /locus_tag="WIC03_001859"
     CDS             complement(2241..>2889)
                     /locus_tag="WIC03_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEZ3884318.1"
                     /translation="VYVFDSLYAASRRNGEPEKTPRPYDANRDGLVIGEGAGIFVLEE
                     LEHAKRRGAIIYAELVGYGANSDAYHISTPRPDAQGAILAFQTALQHAGLAPEDIGWI
                     NLHGTGTHHNDSMESRAVAAVFGNNTPCTSTKPQTGHTLGAAGAIEAAFAWGIADRQS
                     NPEGKLPPQLWDGQNDPNLPAINLTGSGSRWETEKRIAASSSFTFGGSNCVLIIG"
BASE COUNT          662 a          675 c          767 g          785 t
ORIGIN      
        1 tctactatac attgtaacaa agcacttgat gcggcagcac aagccgggga agacgctgca
       61 aaactttgcc aataggctgc gtaatatcgg gggatattgg gattttctta tttttaagga
      121 tggattcggg gtgtttcttt ccaatgaacg aatataattt ggcggtttgt tgcatcaaag
      181 gctttcagac ggcatatgcc gtctgaaagc ggcttatatt gcatccaata atttgtcggc
      241 gatatggttg atggtaaaac ggttggcgac gcattgatgc cctgccgttg ccagtgcgtt
      301 ttttgcttcg gggtttaaga cgaggcgttc gatttcatcc gccaaagatt caggtgtcag
      361 cctgtccagc agaatccccg attgatgatg ttcgataatt tccttttgcg cgcccaaagt
      421 attggaaatc actgccgttc ggcagtacat cgcctcgcat aatgacaaac cgaatgcctc
      481 cgggacgagg ctgggcaaaa ccacgacatc gctttggcgg taaaacgaag caatgtcttc
      541 ggtaaaccct tcaaaagaaa caaatggttc tgctcctgaa gcagatacgt cccgcttcag
      601 gcggcacata taatccggat gtccgtgccc tgccaatttg agtctgattt gaggatattt
      661 ccgatgcagt atcacacagg cttcaatcag gttttccaat cctttttccg gactgatcct
      721 gccggcgtag gcgacggtaa aaaaacggct gtcgggtttt tcttgagagg gagggaaacg
      781 gccggtatcg ataccgttat gaacaatccg gtatttttct ttaaagggat tgtcggcggt
      841 ttgcacatcg taaaccagac gggaaacgca aataaagcgg tctgtgtttt tctgtatcag
      901 gcggtgataa aaatcggttt tgttggcgac gacattgtgt ttgacaaata tcaggcgcac
      961 cccggtcagt tttttcaaaa gtatggataa ggcggcaatt ttgccggtgt gtatcatcag
     1021 gtgggaaatg cggtttttcc gaataaagcg ggtaagcgcg cagagcgaaa acagcccgtt
     1081 gagcgtgtga aggcgtgtcg tgaaaacgga agaaacttcg gaaaagcgtc tgtgcatcaa
     1141 ctcattattt ttattgacgg cggtaaacat cgtgcagccc cgcagtccca atgcttttga
     1201 aacatcatag acatattgtt cgcccccgcc ccaaatcttg gccgatacga tatttaatat
     1261 cctgaattct ttttccattt aaaattttcc gccggatttg tacagataat atagtttgac
     1321 atatttaatc attgtgtaat agctgtggtt gacggacata atccaaccca tttttccatc
     1381 gagaaaccct ttgttcagga tataaatttt gaaaaacccc caaattgggc ggaggataat
     1441 gtccctaacg aaacgcacgg gctttccctg ctctcggtat ttctcggctg aaatggaagt
     1501 atatttgttg aatttgttga aatattgttc ccagttgtcg tacgtataat ggtacataaa
     1561 atgtttcaga cggcgtttag ggtagggggt ctgtacggtt tcgtgtactt tgccttgcac
     1621 cgaactgcct ttcttcggca tcaaacggca tacgctgtcg ggacgcatcg cgccgtgtgt
     1681 ggcgggatgg ttggggaaaa ggttgcggcg ttcgacaaaa taggcggcat aatcgccggt
     1741 ttggacaatt tttgagattt catcagatag ttccggcgtg cagcgttcgt ctgcatcaat
     1801 caggaaaacc cattctccgc ctgcctgttc gatggcaaat gttttttgcg ctccgaaatc
     1861 cccgttcaaa tgccgttgga agacttttgc gcccaaaccc tcggcaattt cggcagtatt
     1921 gtcggtgctg tggtcatcga taacgataac ttctttatcg aaacggcaac tttcaataca
     1981 ttcccgaatg tggtttgctt catttctagc aacaatcaat acgctgactt ttttcattat
     2041 caaggtttct caaagcattt tggtttcaca gggcggcaaa aaagcccaaa catacgtccg
     2101 ggcttgggaa ttggtgggtc cggtggggtt cgaacccacg accaagggat tatgagtccc
     2161 ctgctctaac ccctgagcta cagacccgtc aagagtaggc gcgtattgta tagcatagcg
     2221 gtgggattga caaacttatt tcatccgatg attaagacgc agttgcttcc cccgaaggta
     2281 aacgatgagc tggcggcaat gcgtttttcg gtttcccagc ggctgccgct gccggtcagg
     2341 ttgatggcgg gaaggttcgg atcgttctgc ccgtcccaaa gctggggcgg aagttttcct
     2401 tcgggattgc tttgccggtc ggcaatgccc cacgcgaacg cggcttcgat tgcgcccgcc
     2461 gcacccagcg tgtgtccggt ttgcggcttg gtggacgtgc agggcgtatt gttgccgaaa
     2521 accgctgcaa cggcacggct ttccatactg tcgttgtggt gcgtcccggt gccgtgcaga
     2581 ttaatccagc cgatgtcttc gggcgcaaga cctgcgtgtt gcaatgccgt ctgaaaggca
     2641 aggattgcgc cttgcgcgtc ggggcggggc gtggaaatat ggtaggcatc gctgttggct
     2701 ccgtagccga cgagttcggc gtaaattatc gcaccgcgcc gtttggcgtg ttccaattct
     2761 tccagcacga aaatccccgc gccttcgccg atgaccagcc cgtcgcggtt cgcgtcgtat
     2821 gggcgcgggg ttttttccgg ttcgccgttg cggcggctgg cggcataaag cgagtcgaaa
     2881 acatacact
//
LOCUS       DAWHSK010000236         2881 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.236, whole genome shotgun sequence.
ACCESSION   DAWHSK010000236 DAWHSK010000000
VERSION     DAWHSK010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.236"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..1454
                     /locus_tag="WIC03_001860"
     CDS             <1..1454
                     /locus_tag="WIC03_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEZ3884319.1"
                     /translation="ILAGSGTSLAAPYLDKAAENLGTAGKAAVNALGGAAIGYATGGS
                     GGAVVGANVDWHNRQLHPSERDWIRKNAQAFAKQEVGGREPTIDEIALAEKRLAQQAA
                     KETDLFWMLTLEKVTDLSAQKFLRQAKGTFTNENGTQQQFFTTQGKQFMRPELFANEV
                     YDLSFYKNHLQSPKNSSISKGTGELARRVTSSTWDKAKQDPILTGLQIAASPVHLVRD
                     VGKASWQCVSGFGQCLQQGKENFVETGSAIGTGTASFQLNDLKPIYGQDVRGAQAGLV
                     ALQTADGITTALGAAKALNVAGKGSRYFINEWASSPPSGSRAAQRGAVGNLTHSQQVL
                     TDGSHIQRGGKLLPNIRYKAGEFDYLYETDTLGRIKSFETDNLQLTQRDRLKHNSKTP
                     DKVKGQDHAGHLIGDRFGGSPNLDNLVSQHKDVNLSAYKVLENKWAEAIKSGKNVKIQ
                     MQIEYAAGTRHPTRFVGYYWIDGKPTRININNL"
     gene            1469..1915
                     /locus_tag="WIC03_001861"
     CDS             1469..1915
                     /locus_tag="WIC03_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884320.1"
                     /translation="MIFEDYFSELQADMVSICLEYVQGKADKIYIYAAFEPRVSGAGC
                     FFSVKGKLVQRHLVNQEIPEVNLDHQIQVLDILMEDLEKLNSICQKFNQPMPTEIKMI
                     YDLKKNSLSANYQYNLILSSESGLLALPQDVEDAWFEEIKKQMEDN"
     gene            2071..2421
                     /locus_tag="WIC03_001862"
     CDS             2071..2421
                     /locus_tag="WIC03_001862"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884321.1"
                     /translation="MPTLRLLIPNADSVIIDTRKFTDYALNPNHDVGRHKARVFESAL
                     GYNQSNATELILKIRQGLKDNPARLGKKDQYGQRYTVDIPITGPNGKTAIVTTGWILV
                     PGSEIPKMTTVLVK"
     gene            2431..2622
                     /locus_tag="WIC03_001863"
     CDS             2431..2622
                     /locus_tag="WIC03_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4926 domain-containing protein"
                     /protein_id="HEZ3884322.1"
                     /translation="MNLNDVVKLKVKQPNSNVPEGSIGTIIEVLQELPSAVFEVEFIN
                     KNGELIDTLTLAECDIEPA"
BASE COUNT          990 a          472 c          638 g          781 t
ORIGIN      
        1 gcatactggc cggcagcggc acttcccttg ctgcaccgta tttggacaaa gcggcggaaa
       61 acctcggtac ggcgggcaaa gcggcggtca acgcactggg cggggcggca atcggctatg
      121 caactggtgg tagtggtggt gctgtggtgg gtgcgaatgt agattggcat aatcggcagt
      181 tgcatccgag tgaaagagat tggataagaa aaaatgcaca agcttttgcg aaacaggagg
      241 taggtggtag agaacccact atagatgaaa ttgcattagc agaaaaacga ctagcacaac
      301 aggcggctaa agagacagat ttgttttgga tgctaacatt agaaaaagta acagatttat
      361 cagctcaaaa attcctaaga caagcaaaag gaacttttac aaatgaaaat ggtacgcagc
      421 aacaattttt tactacgcaa gggaagcaat ttatgcgtcc tgaattgttt gctaatgaag
      481 tatatgatct gtctttttac aaaaaccatt tacaatctcc taaaaatagt agtatcagca
      541 agggtacagg agaattggct cgaagagtta cttcatctac ttgggataaa gcaaaacaag
      601 atcctatttt gacaggattg caaatagctg cctcccctgt acatttagtt cgggatgtag
      661 gtaaggcaag ctggcagtgc gtaagcggct ttggacaatg tttgcaacaa ggaaaagaga
      721 attttgtaga aacaggttct gcaattggaa caggaactgc ctcttttcaa ctaaacgacc
      781 ttaagccgat ttatggtcaa gatgtacgtg gtgcacaagc aggattggtt gcactacaaa
      841 cggcagatgg aataactacg gcattgggtg cagcaaaagc attaaatgtt gcaggaaaag
      901 gtagtcgcta ttttataaat gaatgggcta gtagtccgcc tagtggttct cgtgctgcac
      961 agcgtggggc agtggggaat ttaacccatt cgcaacaagt tttgacagat ggttcacata
     1021 ttcaacgtgg cggaaaactt ttacctaata ttcgctataa agcaggcgag tttgattatt
     1081 tatatgaaac tgatacttta ggtagaatta aaagttttga aacagataat ttgcaattaa
     1141 cacagagaga tagattaaag cataattcaa aaactcctga taaagtcaag gggcaagacc
     1201 atgcaggaca tttaattgga gatagatttg gaggttcgcc caatttagat aatttagtgt
     1261 cacagcacaa agatgtaaac ttaagtgcgt ataaagtttt ggagaataaa tgggcggaag
     1321 caattaaatc aggaaaaaat gttaaaatcc aaatgcaaat tgaatatgca gcaggtacaa
     1381 gacaccctac acgatttgtt ggatattatt ggattgatgg taaaccaact agaattaata
     1441 ttaataattt atgaaacaag gattttttat gatttttgaa gattatttta gtgaattaca
     1501 agcagatatg gtttctattt gtttggaata tgttcaaggt aaggctgata aaatttacat
     1561 ttatgcagct tttgagcctc gtgttagtgg tgctggatgt tttttctctg ttaaaggaaa
     1621 acttgtacaa cgacatcttg ttaatcaaga aattccagaa gttaatcttg accatcaaat
     1681 tcaagtatta gacatactta tggaagattt agaaaaacta aattctattt gtcagaaatt
     1741 taaccaacct atgccaactg aaattaaaat gatttatgat ttaaaaaaga atagcttatc
     1801 agcaaattat caatataatt taatcttgtc atctgaatct ggattacttg ctttaccgca
     1861 agatgttgaa gatgcatggt ttgaagaaat taagaagcaa atggaagata attaattcac
     1921 agcaagcgta gcaagcgtag ggtgggcaac tcgttgccca cgcagttcaa tcgtatatgt
     1981 taaaatgatg ttttaatgtg aaatatttca tttcaggctg cctgaaaaaa taaaataacg
     2041 tggcgtgtat caaaccgcgt gggcaacgag ttgcccaccc tacgcttgct aatccctaac
     2101 gctgattctg tcataatcga taccagaaaa tttacagatt atgcattgaa tcctaatcat
     2161 gatgttggga gacataaagc tagagttttt gaatcagctc taggatataa tcaatctaat
     2221 gcaactgagc taattttgaa aattagacaa ggattaaaag ataaccctgc aagattagga
     2281 aaaaaagatc aatatgggca aagatataca gtagatattc ctattactgg acctaatggg
     2341 aaaacagcca ttgtaactac aggctggatt ttagtgcctg gatctgaaat tcccaaaatg
     2401 acaaccgttt tagtaaaata ggtgccaata atgaatttaa atgatgttgt aaagcttaaa
     2461 gtaaaacagc ccaatagtaa tgtacctgaa ggtagtatag gtacaataat cgaagttctt
     2521 caggaattgc catctgctgt ttttgaagtg gaatttataa ataaaaatgg cgaactaatc
     2581 gatacattaa cgctagcaga atgtgacata gagccagcgt aacctgtgca attaatgcga
     2641 actgttacat ggtagggtgg gcaactcgtt gcccacgcag ttcagtctca tgtgctaaaa
     2701 ctatgtttta aggcgaaagt tttggattgg gcgcaagtgc gtccataagc ggcaaaacac
     2761 tgggacaggg cgcaaaaaat aaacctcaaa acaaacacct gacaagcgtg gccgataaaa
     2821 acggcgcaag ttcatcagtg ggttatggca gcgaccaaga cagtcaaagc agcatcacga
     2881 a
//
LOCUS       DAWHSK010000237         2879 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.237, whole genome shotgun sequence.
ACCESSION   DAWHSK010000237 DAWHSK010000000
VERSION     DAWHSK010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.237"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            82..1329
                     /locus_tag="WIC03_001864"
     CDS             82..1329
                     /locus_tag="WIC03_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEZ3884323.1"
                     /translation="MLKQTTLLAACTAVAALLGGCATQQPAPVIAGNSGMQDAPSSAV
                     YNNPYGATPYSPAPAGDAPYVPPVQSAPVYTPPAYVPPSAPAVSGTYVPSYAPVDINA
                     ATHTIVRGDTVYNISKRYHISQDDFRAWNGMTDNTLSIGQIVKVKPAGYAAPKAAAVK
                     SRPAVPAAAQPLVQSAPVDINAATHTIVRGDTVYNISKRYHISQDDFRAWNGMTDNML
                     SIGQIVKVKPAGYAAPKTSAVESRPAVPAAVQTPVKPAAQPPVQSAPQPAAPAAENKA
                     VPAPAPQSPAASPSGTRSVGGIVWQRPTQGKVVADFGGNNKGVDIAGNAGQPVLAAAD
                     GKVVYAGSGLRGYGNLVIIQHNSSFLTAYGHNQKLLVGEGQQVKRGQQVALMGNTDAS
                     RTQLHFEVRQNGKPVNPNSYIAF"
     gene            complement(1536..2018)
                     /locus_tag="WIC03_001865"
     CDS             complement(1536..2018)
                     /locus_tag="WIC03_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEZ3884324.1"
                     /translation="MTQQRQLPSHELIMSELMMPDTANFSGNVHGGELLLLLDQVAYS
                     CASRYSGNYCVTLSVDKVLFKEPIHIGDLVTFYAAVNYTGRTSMEIGIRVEAQNIRTG
                     EIRHTNSCYFTMVAVKDGKPVPVPPLEILTDRQRCRYEKAKKRRDISLQASEDMSCGC
                     "
     gene            complement(2171..2332)
                     /locus_tag="WIC03_001866"
     CDS             complement(2171..2332)
                     /locus_tag="WIC03_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884325.1"
                     /translation="MSGQPEKHHTSPIEDERKNPVYRMGRAVAGFMLVVWVAVLALVF
                     FLVFRFWLS"
     gene            complement(2406..2867)
                     /gene="recX"
                     /locus_tag="WIC03_001867"
     CDS             complement(2406..2867)
                     /gene="recX"
                     /locus_tag="WIC03_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEZ3884326.1"
                     /translation="MKPQKSLRARAMDILSRQEVSRIGLKRKLAPHAESEEELENVLN
                     EFAERNWQSDLRYAEAYIRSKSRKHGSLRLKQALAQQGIDEETSRNLLPDRSSEKQAA
                     IAVLRKKFKHPAADLKEKQKQARFLAYRGFDADTVQTALKHAWDENWEDGC"
BASE COUNT          615 a          727 c          828 g          709 t
ORIGIN      
        1 ggctttgcgc ttgagaagaa cgcgcatttt tattatgata taccgcattc aggctatata
       61 tcggaacatg aaggttatct tatgttgaaa caaacgacac ttttggcagc ttgtaccgcc
      121 gttgccgctc tgttgggcgg ttgcgccacc caacagcctg ctcctgtcat tgcaggcaat
      181 tcaggtatgc aggacgcgcc gtcttcggca gtttacaaca acccctatgg agcaacgccg
      241 tacagcccgg ctcctgccgg cgatgcgcct tatgtgccgc cggtgcaaag cgcgccggtt
      301 tatacgcctc ctgcttatgt tccgccgtct gcacctgccg tttcgggtac atacgttcct
      361 tcttacgcac ccgtcgacat caacgcggcg acgcatacta ttgtgcgcgg cgacacggtg
      421 tacaacattt ccaaacgcta ccatatctct caagacgatt tccgtgcgtg gaacggcatg
      481 accgacaata cgttgagcat cggtcagatt gttaaagtca aaccggcagg atatgccgca
      541 ccgaaagccg cagccgtaaa aagcaggccc gccgtaccgg ctgccgcgca accgctcgta
      601 cagtccgcac ccgtcgacat caacgcggcg acgcatacta ttgtgcgcgg cgacacggtg
      661 tacaacattt ccaaacgcta ccatatctct caagacgatt tccgtgcgtg gaacggcatg
      721 accgacaata tgttgagcat cggtcagatt gttaaagtca aaccggcagg atatgccgca
      781 ccgaaaacct cagccgtaga aagcaggccc gccgtaccgg ctgccgtgca aacccctgtg
      841 aaacccgccg cgcaaccgcc tgtgcagtcc gcgccgcaac ctgccgcgcc cgctgcggaa
      901 aataaagcgg ttcccgcgcc cgccccgcaa tctcctgccg cttcgccttc cggaacgcgt
      961 tcggtcggcg gcattgtttg gcagcgtccg acgcaaggta aagtggttgc cgatttcggc
     1021 ggcaacaaca agggtgtcga tattgcagga aatgcgggac aacccgtttt ggcggcggct
     1081 gacggcaaag tggtttatgc cggttccggt ttgaggggat acggaaactt ggtcatcatc
     1141 cagcataatt cttccttcct gaccgcatac gggcacaacc aaaaattact ggtcggcgaa
     1201 ggtcagcagg tcaaacgcgg tcagcaggtt gccttgatgg gcaataccga cgcttctaga
     1261 acgcagcttc atttcgaggt gcggcaaaac ggcaaaccag ttaatccgaa cagctatatc
     1321 gcgttctgac tttctgcctg aaatcaagtt ggaaatgccg tctgaaaggt tttcagacgg
     1381 catttttgtt tttggcggct gtgttttcaa atcattcgga agggacgggg catataaatt
     1441 tgtttgactg tgtttgggta tttgcttgat ttcatccgtt tgcagtagaa acggatggcg
     1501 cgatgtgcct gtctttcaga cggcatagtc cgccgtcagc agccgcagga catgtcttca
     1561 gaggcttgca ggctgatgtc tctgcgtttt ttggcttttt cgtagcggca gcgttggcgg
     1621 tcggtcagga tttccagcgg agggacaggg acgggtttgc cgtctttgac cgcaaccatc
     1681 gtgaagtagc agctgttggt gtggcggatt tcgccggtgc ggatgttttg tgcttcgaca
     1741 cggatgccga tttccatcga ggtgcgtccc gtgtagttga cagcggcgta aaaggtaacc
     1801 aaatcgccga tgtggatggg ttctttaaac aggactttgt caaccgacag ggtaacgcaa
     1861 taattgccgc tgtaacggct ggcgcaggaa taggcgactt ggtcgagcag gagcaggagt
     1921 tcgccgccgt gtacgttgcc gctgaaattg gcggtgtccg gcatcatcag ttcggacata
     1981 atgagttcgt gggaaggcag ttggcgttgt tgtgtcatgg tgtacctgtt ttaagaaagg
     2041 gtgttttgaa atgggtgcgg ggaaatttta gggaatgggg aagtggaatt caagctaaaa
     2101 aaagctatga tgggatagtc tgccgaaatg gaccgccgat gccgtctgaa gccttcagac
     2161 ggcattttgt ttaggaaagc caaaaacgga agaccaggaa aaacacgagt gccaatacgg
     2221 cgacccaaac gacgagcatg aatccggcaa ctgcccgtcc catacggtaa accgggtttt
     2281 tgcgttcgtc ttcgatgggg gaggtgtggt gtttttcggg ttgtccggac attttgttct
     2341 caatcggaaa agttggaaac gggcgtaagg ttatgttgcc gtgcaaaaag attcaagaag
     2401 gaaggtcagc agccgtcttc ccaattttcg tcccaagcgt gtttcaatgc cgtctgaacg
     2461 gtatcggcat cgaatccgcg ataggcgagg aaacgcgcct gtttttgttt ttctttgagg
     2521 tcggcggcgg ggtgtttgaa ttttttacgc aacacggcta tggcggcctg tttttcgctt
     2581 gagcggtcgg gaagcaggtt gcggctggtt tcttcatcta tgccctgttg cgccaaagcc
     2641 tgtttcagcc tcaatgaacc gtgtttgcgg cttttgctgc ggatataggc ttcggcatag
     2701 cgcaaatccg actgccagtt gcgttcggca aattcgttta acacgttttc caactcttct
     2761 tcgctttcgg cgtgcggtgc aagtttgcgt ttcagaccga tgcggctgac ttcttggcgc
     2821 gagaggatgt ccatcgcgcg ggcgcgtagg gatttttgcg gtttcatggc ggcggtttc
//
LOCUS       DAWHSK010000238         2871 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.238, whole genome shotgun sequence.
ACCESSION   DAWHSK010000238 DAWHSK010000000
VERSION     DAWHSK010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.238"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            435..1658
                     /locus_tag="WIC03_001868"
     CDS             435..1658
                     /locus_tag="WIC03_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HEZ3884327.1"
                     /translation="MSAQSQNNHTSPLVVLTTLFFMMGFITCMNDILIPHLKEIFDLS
                     YVQAMLIQFCFFTAYAVMSIPMGAFVGKVGYKNGVIGGFLLTAVGCLLFYPAAGSHSY
                     AVFLGALFILASGVTLLQVAGNPYVTLLAKPGKESATLTLVQAFNALGTTIAPQIGAF
                     LILADATQTVSKAEQISSVQIPYLGLAGLLIILAVFVKMIRLPDARKIAAEESAHNHD
                     GKTSVWQYKHLVFGTAGIFCYVGAEVSIGSLMVNVLGYLKGLDHASAAHYLSFYWGGA
                     MVGRFLGSAVMAKFAPNRYLAFNASAAVVLLAVAMATGSGNADVAMWSLLAIGFFNSI
                     MFPTIFSLATKGLGKFTNAASGVLCTAIVGGAVVPVVQGWVADTYTLMSSFVVSVICY
                     LYIVFFAVYGYRADK"
     gene            1949..>2871
                     /locus_tag="WIC03_001869"
     CDS             1949..>2871
                     /locus_tag="WIC03_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEZ3884328.1"
                     /translation="MFAFKSLLDMPRGEALAVVVALIAAMGYTIISLEWLPHMSIIAA
                     IVVLILYGLVRGLKYNDMQQGMIGALNQGMGAIYLFFFIGLMVSALMMSGAIPTLMYY
                     GFGLISPTYFYFSAFALCSVIGVSIGSSLTTCATVGVAFMGMAAAFHADMAMTAGAIV
                     SGAFFGDKMSPLSDTTGISASIVGIDLFEHIKNMMYTTIPAWLISAALMLWLLPSVAA
                     QDLNSVESFRSQLEATGLVHGYSLIPFALLVVLALMRVNAVVAMLFTVMVAVAVTYLH
                     STPDLRQLGAWFYGGYKLEGEAFKDIAKLISR"
BASE COUNT          582 a          702 c          744 g          843 t
ORIGIN      
        1 cgtcgccttg tcctgatttt tgttaatcca ctatattttc acgcccccgc cgaagggcgg
       61 aggacggtgc aaaaaatacg gcacagccgt atgccccttt tttgtcgggc atacgacatt
      121 ctttccgctc cggttttgat gccacgatgc ggcatttccg aattttccgg atacggcggc
      181 ggattttcat tttattggga acggtttttg caagtccgcc ggaatttttt aaaatctatt
      241 aaaatctatt aaaatctatg caagcaactg taaaatatta atttctgctg cttgaatttc
      301 agatcggcgc attgcctgca tccgataaag tttgcaaaat gttcaaatat cagtatgatt
      361 tgcattgccg ttaagaaatg tcaatttcta ttttcttgaa acgggtaata ttccgacacc
      421 acgaaaggca aatcatgtct gcgcaatcac aaaacaatca tacgtcccca ttggtcgtct
      481 tgaccacgct gttcttcatg atgggtttta ttacctgcat gaacgacatc cttatccctc
      541 atttgaaaga aattttcgac ctgtcttacg ttcaggcgat gctgatccaa ttctgtttct
      601 ttaccgccta tgcggtgatg tccatcccga tgggggcttt tgtcggcaaa gtcggctaca
      661 aaaacggcgt tatcggcggc tttctgctga cggcggtcgg atgcctgctg ttttatcctg
      721 ctgcgggtag ccattcttac gcggtatttt tgggcgcgtt gtttattttg gcttccggcg
      781 taacgctgct tcaggtcgcc ggtaatcctt atgttaccct gctggcgaaa cccggcaagg
      841 aatcggcaac actgacgctg gttcaggcgt ttaacgcttt gggtacgacc attgcgccgc
      901 aaatcggcgc gttcctgatt ctggcggacg caacccaaac cgtcagcaag gcggaacaga
      961 tttcttccgt acagattccc tatttgggac tggcggggct gctgattatc cttgccgttt
     1021 tcgtgaaaat gatccggctg cccgacgcgc gcaaaattgc cgccgaggaa agcgcgcaca
     1081 accacgacgg caaaaccagc gtatggcaat acaaacatct cgtgttcggt acggcaggca
     1141 ttttctgcta tgtcggcgcg gaggtgtcta tcggttcgtt gatggtcaac gtattgggtt
     1201 atctgaaagg gctggatcat gcttctgccg cgcattacct gtcgttctat tggggcggcg
     1261 cgatggtcgg acgtttcctc ggttcggcgg tgatggcgaa attcgcgccc aaccgttatt
     1321 tggcgtttaa cgcatcggct gcggtcgtac tgcttgccgt cgcgatggcg acgggtagcg
     1381 gcaatgcgga tgtggcgatg tggtcgctgc ttgccatcgg ttttttcaac tcgattatgt
     1441 tcccgacgat tttctctttg gcaaccaaag gattgggaaa atttaccaac gcggcttccg
     1501 gtgtactgtg taccgcgatt gtcggcggtg cggtcgttcc tgtcgtgcag ggctgggtgg
     1561 cagatactta caccctgatg tcttcgtttg tcgtttccgt catctgttat ctgtatatcg
     1621 tgttttttgc ggtgtacgga tatagggcgg acaaataatc tttttcttga gaaatgtcgt
     1681 ctgaacatct ttcagacggc atttttgcgt accggtgttt gcggcgtgtg tgccgaggtt
     1741 ttaatacttc aatccataaa agtcttatat gccaacaaac aaaaaaataa aaaattatat
     1801 ttcaaaaaaa ttaatttaaa ttgagaaaat tgccgttttg tttctgtccg gcttttgtaa
     1861 aacgctaaaa tgccgtctga aaacgttggg cggattcggt atggtgtgtt agaatccgtt
     1921 aactttatat caaatcgggc aaagaatcat gtttgctttc aaatccttac tcgatatgcc
     1981 gcgcggtgag gcacttgccg tcgtcgtcgc tctgattgcc gcgatgggct ataccatcat
     2041 ttcattggag tggctgccgc atatgtccat tattgccgcc atcgtcgtgc tgattttgta
     2101 cggcttggtg cgcggtttga aatacaacga tatgcagcag ggcatgatag gcgcgttgaa
     2161 tcagggtatg ggcgcgattt acctgttttt cttcatcggg ctgatggtca gcgcgctgat
     2221 gatgagcggc gcgattccga cgctgatgta ttacggtttc gggctgattt ccccgactta
     2281 tttttatttt tccgccttcg cgctgtgttc cgtcatcggc gtgtccatcg gcagcagcct
     2341 gaccacctgc gccaccgtcg gcgttgcctt tatggggatg gcggcggcgt ttcatgccga
     2401 tatggcgatg acggcgggcg cgattgtttc cggtgcgttt ttcggcgaca aaatgtcccc
     2461 gctttccgac accacgggca tttccgcgtc catcgtcggc atcgacctgt ttgagcacat
     2521 caaaaatatg atgtacacca ccattcccgc gtggctcatc agtgcggcac tgatgctgtg
     2581 gcttcttccc agtgtcgccg cgcaggattt gaacagcgtc gaatccttcc gcagccagct
     2641 tgaagccacg ggattggtgc acggctattc gctgattccg tttgcactgt tggtcgtttt
     2701 ggcattgatg cgcgtcaatg ccgtggtcgc catgctcttt accgtcatgg ttgccgttgc
     2761 cgtaacgtat ctgcacagta cgcccgatct gcgtcagctc ggcgcgtggt tttacggcgg
     2821 ctacaaactc gaaggcgaag cgtttaaaga cattgccaaa ctcatttcgc g
//
LOCUS       DAWHSK010000239         2804 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.239, whole genome shotgun sequence.
ACCESSION   DAWHSK010000239 DAWHSK010000000
VERSION     DAWHSK010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.239"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            141..722
                     /locus_tag="WIC03_001870"
     CDS             141..722
                     /locus_tag="WIC03_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEZ3884329.1"
                     /translation="MEEKNDYTDAASDNRNGQEIEVGIAGAGDRILAALLNQLFTFLV
                     LLVPFAGLIAFAIKNEGRIGGSEEIFGLLLGMTSFWVGLAGILVYTVVQIYYMSRDGQ
                     SLGKKIMRIRVLKTDGRNPGFVGTVLVREIAWSVLVAIIAAVIGLAVGDNGENAINLL
                     AFLANFVLLFMVKRDRRTLYDILADTVVVKLPK"
     gene            complement(784..1638)
                     /gene="folD"
                     /locus_tag="WIC03_001871"
     CDS             complement(784..1638)
                     /gene="folD"
                     /locus_tag="WIC03_001871"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEZ3884330.1"
                     /translation="MSAQLINGKEVSQKRLQAVAEAVAQRQQDNLHMPCLAVVLVGGD
                     PASAVYVRNKKTACQKCGIKSLSYELPESTSQEELLALVDRLNADSEVDGILVQLPLP
                     KHLDSQAVLERISPDKDVDGFHPYNVGRLAVKMPLMRPCTPKGVMTLLEAYGIDPKGK
                     KAVVVGASNIVGRPQALELLLARATVTVCHSATENLTDEVAGADILVVGVGIPNFVKG
                     EWIKPGAVVIDVGINRLDDGSLCGDVEFETAKERAAMITPVPGGVGPMTIATLMENTL
                     HAASLHDA"
     gene            1861..1938
                     /locus_tag="WIC03_001872"
     tRNA            1861..1938
                     /locus_tag="WIC03_001872"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1896..1898,aa:Pro,seq:tgg)
     gene            1963..2039
                     /locus_tag="WIC03_001873"
     tRNA            1963..2039
                     /locus_tag="WIC03_001873"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1997..1999,aa:Arg,seq:tct)
     gene            2067..2142
                     /locus_tag="WIC03_001874"
     tRNA            2067..2142
                     /locus_tag="WIC03_001874"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2100..2102,aa:His,seq:gtg)
     gene            2353..>2804
                     /locus_tag="WIC03_001875"
     CDS             2353..>2804
                     /locus_tag="WIC03_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional heptose 7-phosphate kinase/heptose
                     1-phosphate adenyltransferase"
                     /protein_id="HEZ3884331.1"
                     /translation="MVDAWSVPDFESKICPPEALAARLALLPRPLVFTNGCFDILHRG
                     HVTYLAQARSAGAALVLALNTDASVRRLGKGGDRPINPLENRAAVAAALESVDLVTWF
                     DGDTPAALIEAVKPEILVKGGDWAADKIVGAAETLARGGQVFSIPFLH"
BASE COUNT          575 a          707 c          787 g          735 t
ORIGIN      
        1 tatgtatggc gtaaaggaag cgttttaacg ccgtattggg aaaatgtaaa gttctttatc
       61 gggatttctt taagtattgg cgttttcggt gtcgtggcgg tagaatcggg gtcagttaag
      121 aacaattatc aggaacggat atggaagaaa aaaacgacta caccgacgcg gcatccgaca
      181 accgcaacgg gcaggaaatt gaagtcggaa ttgccggcgc aggcgaccgg atacttgccg
      241 cgctgctgaa ccaattgttt acctttttgg ttttgttggt gccgtttgcc ggactgattg
      301 cttttgccat caagaatgaa ggcaggattg gcggcagtga ggaaatattc gggctgcttt
      361 tgggcatgac ttccttttgg gtaggactgg cgggcatttt ggtttatacc gtcgttcaaa
      421 tctactatat gagtcgggac gggcagtcat tgggtaagaa aatcatgaga atccgcgtgt
      481 tgaaaaccga cggccgcaat cccggttttg tcggcacggt tttggtacgc gaaatcgcat
      541 ggtcggtttt ggttgccatt attgccgccg ttatcggtct tgcagtaggt gacaacggag
      601 aaaacgccat caacctgctg gcattccttg ccaactttgt cctgctcttt atggtcaaac
      661 gcgaccgccg cacgctttac gacatactgg cggatacggt tgtcgtcaag ctgcccaaat
      721 aaacggacac ggcaaaatgc cgtctgaaag cctttcagac ggcattttta tcttcagaac
      781 cgcttaagca tcgtgcagcg aagccgcgtg cagagtgttt tccattaatg tggcgattgt
      841 catcggaccc acgccgccgg gaacgggcgt aatcatcgcc gcccgttctt ttgccgtttc
      901 aaattccacg tcgccgcaca ggctgccgtc gtccaaacgg ttgatgccca catcgataac
      961 gaccgcgccg ggtttgatcc attcgccttt gacaaagttc ggaatgccca cgccgaccac
     1021 caaaatatcg gctccggcaa cctcgtctgt cagattttcg gttgcgctgt ggcagaccgt
     1081 taccgttgcg cgcgccagca gcagttccaa agcctgcggg cggccgacga tattcgacgc
     1141 gccgaccacg accgcttttt tccccttcgg atcaatgccg taagcttcca aaagcgtcat
     1201 cacgcccttg ggcgtacacg ggcgcatcag cggcattttg accgccagcc tgccgacatt
     1261 gtaaggatgg aagccgtcca cgtccttatc cggcgaaata cgttccaaaa ccgcctggct
     1321 gtcgaggtgc ttcggcagcg gtagctgaac cagaataccg tccacttcgg aatcggcatt
     1381 caggcggtcg accagtgcca gcagttcttc ctgcgatgtt gattcgggca gctcgtaaga
     1441 cagtgatttg atgccgcatt tttggcaggc agttttcttg ttgcggacat aaaccgcgct
     1501 ggcagggtcg cctccgacca aaaccacggc caggcaaggc atgtgcagat tgtcctgttg
     1561 gcgttgcgcc accgcttcgg caaccgcctg caggcgtttt tgcgaaactt ctttaccatt
     1621 gatcagttgt gccgacatat ccgtcctttt gttaaacagg tagaaaatgc tgaaaattat
     1681 cgcattttcc gtttgttttt acagcaggat gccgaaaata aatttgccct gaaataaaat
     1741 acggctgttt ggacggatta aatatttttt caaatcaaaa aactatattt ttcatcttgg
     1801 tgtcttgaaa ataaatattg acgcatcttt ccctgagttg tatagttcgc ttcttcaagt
     1861 cggggcgtag cgcagcccgg ttagcgcatc tgctttggga gcagagggtc gtgagttcga
     1921 atcccaccgc cccgaccaat caatcagcac gaatcggcat gagcgcccgt agctcaaccg
     1981 gatagagcac cgaccttcta agtcgggggt tacaggttcg attcctgtcg ggcgtgccaa
     2041 gaatttgatt gaaaaccgtt tttatggtgg ctgtagctca gttggtagag ccccggattg
     2101 tgattccggt tgtcgtgggt tcgagcccca tcagccaccc cagataaagg cccgtacttt
     2161 tcggtacggg tctttttccg tttgcacgta ctgtccgcac cgtcgtaccg gtgcggcggt
     2221 atgccgtctg aaagccgggc cggtcatacg gaaaccgctt cagacggcat ttgcgccgcc
     2281 ggctttgcct ttcgcatatg cttctgtatc atgtaggttt tctgtctttt atggtttttg
     2341 tcggagactt tgatggttga cgcttggtct gttcctgatt ttgaatcgaa aatctgtccg
     2401 cccgaggcgt tggcggcgcg tttggcgttg ttgccgcgcc cgctggtgtt taccaacggc
     2461 tgtttcgaca tcctccacag ggggcatgtt acttatctgg cgcaggcgcg ttcggcaggg
     2521 gcggcgttgg tgctggcgtt gaataccgat gcttcggtgc ggcgtttggg caagggcggc
     2581 gaccgcccga ttaatccttt ggagaaccgt gccgccgttg ccgccgcttt ggaaagtgtg
     2641 gatttggtaa cgtggtttga cggggatacg ccggcggcgt tgattgaggc ggtcaaacct
     2701 gagattttgg tcaagggcgg cgattgggct gcggataaga ttgtcggtgc ggcagaaacg
     2761 ttggcgcgcg gcggtcaggt gttttcaatt ccgtttctgc acca
//
LOCUS       DAWHSK010000240         2756 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.240, whole genome shotgun sequence.
ACCESSION   DAWHSK010000240 DAWHSK010000000
VERSION     DAWHSK010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2756
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.240"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            110..805
                     /gene="pgl"
                     /locus_tag="WIC03_001876"
     CDS             110..805
                     /gene="pgl"
                     /locus_tag="WIC03_001876"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEZ3884332.1"
                     /translation="MFVWHEYENAAEAAQSLADAVADALQGALDEKGGAVLAVSGGRS
                     PIAFFNALSQKDLDWKNVGITLADERIVPTVHADSNTGLVREYLLKNKAEAAMWIPMV
                     EDGKTETELHPDAVVDYALKHYKQPDVLVLGMGNDGHTASIFPKAPQFQTAIDGSAGV
                     ALVHTTPVTAPHERVSMTLDAIAHTGHVFLAIRGEEKKAVFDQAAQGENREYPINLVL
                     NHQGVNCHVFYAE"
     gene            786..1772
                     /locus_tag="WIC03_001877"
     CDS             786..1772
                     /locus_tag="WIC03_001877"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEZ3884333.1"
                     /translation="MSSTPNKQAGYPRLVADIGGTNARFALETAPRVIEKAAVLPCKD
                     YDTVTDAVRAYLNQSGATAVRHAAFAIANPILGDWVQMTNHHWAFSIETTRQTLGLDT
                     LILLNDFTAQALAVTQTSSKDLMQVGGQKPVEFAPKAVIGPGTGLGVSGLVHSHAGWV
                     ALAGEGGHTSFPPFDDMEVLIWQYAKNKYGHVSAERFLSGAGLSLVYEALAAKQKAKP
                     AKLMPSEITEKALSGASPLCRQTLDIFCAMLGTVASNLALTLGARGGVYLCGGIIPRV
                     LEYFKTSPFRSRFENKGRFEAYLAAIPVYVVLSEFPGISGAAAALDNHLRNV"
     gene            1822..2670
                     /gene="hexR"
                     /locus_tag="WIC03_001878"
     CDS             1822..2670
                     /gene="hexR"
                     /locus_tag="WIC03_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HEZ3884334.1"
                     /translation="MLSKISESLANLSGAERKVAECALAEPKWFVHAAVAEIAERASV
                     SQPTVIRFCRSLGYKGLPEFKLALSASIGHEGMPYVHEELNADDDMASVVEKVLGNAA
                     ASLLGERRFLKESELENAIATLMHARRVEFYGVGNSGIVAQDAQHKFFRFGMSTVAYV
                     DTHTQLMAASVLSDQDVLVAISNTGSSIELLDAVSIAKENGASVIALTRNDSPLAQLA
                     DCVLSVATQENAELYTPMVSRLLQLAVIDILAIGLALRLGDAASLQLQKSKKSIHNKH
                     IDYDKD"
BASE COUNT          628 a          759 c          767 g          602 t
ORIGIN      
        1 gcagtcccga tatttggtat caaaagcata aacaactgtt cggccgacat attgctcaac
       61 ccgtttccat aacggaatat gccgtctgaa ataaaaaaag gacacaaata tgtttgtttg
      121 gcacgaatac gaaaatgcgg cagaagcggc gcagtctttg gctgacgcag tggcggatgc
      181 tttgcagggc gcactggacg agaagggcgg tgcggtgttg gcagtttccg gcggacgttc
      241 gccgattgca tttttcaacg ccctgtcgca aaaagatttg gattggaaaa acgtcggcat
      301 caccttggca gatgaacgca tcgtgccgac cgtccacgcc gacagcaata ccggtttggt
      361 gcgcgaatac ctgttgaaga acaaagcgga agcggcaatg tggattccta tggtggaaga
      421 cggaaaaact gaaaccgaat tacatcccga tgctgttgtc gattatgcac tgaaacatta
      481 caaacagccc gatgttttgg ttttgggtat gggaaacgac gggcatacgg cttcgatttt
      541 cccgaaagct ccgcagtttc agacggcaat cgacggttcg gcaggtgtcg cgctggtgca
      601 taccacgccc gttaccgcgc cgcacgagcg cgtcagtatg accttggatg cgattgccca
      661 tacggggcat gtgtttttgg cgatacgggg cgaagagaaa aaagccgtgt tcgaccaagc
      721 cgcacaaggc gaaaaccgcg aatatccgat caacctcgtt ttgaaccatc aaggagtgaa
      781 ctgccatgtc ttctacgccg aataaacaag ccggatatcc ccgactggtc gccgatatcg
      841 gcgggacgaa tgcacgcttt gcgctggaaa ccgcgccgcg cgtcattgaa aaagccgccg
      901 tgcttccgtg taaagactac gatacggtta ccgatgcggt gcgtgcctat ctgaatcaaa
      961 gcggtgcaac agccgtacgg cacgcggcat ttgccatcgc caacccgatt ttgggcgact
     1021 gggtgcagat gaccaaccac cattgggcgt tttccatcga aaccacccgt cagactttgg
     1081 ggctggacac cctcatcctt ttgaacgact ttaccgcgca ggcattggcg gtaacgcaga
     1141 cttcaagcaa agacctgatg caggtaggcg ggcaaaagcc tgtcgaattt gcccccaaag
     1201 ccgttatcgg ccccggtacc ggcctgggcg tgagcggatt ggtgcacagc cacgcaggct
     1261 gggtggcttt ggcgggcgag ggcgggcata ccagtttccc gccgtttgac gatatggaag
     1321 tgctgatttg gcagtacgcc aaaaacaaat acggccatgt ttccgccgaa cgctttttga
     1381 gcggcgcggg cttgagcttg gtttacgagg ctttggctgc aaaacagaaa gccaaacccg
     1441 ccaaactgat gccgtctgaa atcacggaaa aggctttgag cggcgcgtcg cctttgtgcc
     1501 gtcagacttt ggacatcttc tgcgccatgc tcggcacggt tgcttccaac ctcgccctga
     1561 cgctgggcgc gcgcggcggc gtgtacctgt gcggcggcat tattccccgc gtgttggaat
     1621 atttcaaaac ttccccgttc cgcagccgtt tcgagaacaa gggcaggttt gaagcatatc
     1681 ttgccgcgat tcccgtgtat gtcgtcttga gcgagtttcc cggaatttcc ggtgcggctg
     1741 cggctctcga caaccatttg agaaacgttt aaccacagcg gctccttgca gcggggctgc
     1801 attatcgaag ggcatatcat tatgttaagc aaaatcagcg aatcactggc aaacctttcc
     1861 ggtgcggaac gcaaagtcgc cgaatgtgca ttggcggaac ccaaatggtt cgtccatgcc
     1921 gccgttgccg aaattgccga acgtgcgtcc gtcagccagc cgaccgtcat ccgattctgc
     1981 cgcagcttgg gttataaagg gctgcccgag ttcaagctcg ccttgtccgc cagcatcggt
     2041 catgagggta tgccctatgt ccacgaagaa ctcaacgccg acgacgatat ggcaagcgtg
     2101 gtcgagaaag tgttgggcaa tgccgccgcc tcgctgttgg gcgaacgccg cttcctgaaa
     2161 gagtcggagc tggaaaacgc cattgccacg ctgatgcacg cccgtcgcgt cgagttttac
     2221 ggtgtcggca attccggcat tgtggcacag gacgcgcagc ataaattttt ccgtttcggc
     2281 atgtccaccg tcgcctatgt cgatacgcac acgcagctga tggcggcatc tgttttgagc
     2341 gatcaggatg ttttggttgc catttccaac acgggttcgt ctatcgaact tttggatgcg
     2401 gtcagcatcg ccaaagaaaa cggcgcgtct gtcatcgcac tgacccgcaa cgattcgcct
     2461 ctggcgcaac ttgccgactg cgtgttgagc gttgccacac aggaaaatgc cgaactctac
     2521 acgcccatgg tttcccgcct cttgcagctt gccgtcatcg acattctcgc catcggactt
     2581 gccctgcgct tgggcgatgc tgccagcctg caattgcaga aaagcaaaaa aagcatacac
     2641 aacaagcaca tcgattacga caaagattga ccttcagacg gcatcccaca aatgccgtct
     2701 gaaatgccga acaacggtcg tcggcggctt gcggcagttt ccggcagcct tttcat
//
LOCUS       DAWHSK010000241         2702 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.241, whole genome shotgun sequence.
ACCESSION   DAWHSK010000241 DAWHSK010000000
VERSION     DAWHSK010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.241"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            31..>2702
                     /gene="frpC"
                     /locus_tag="WIC03_001879"
     CDS             31..>2702
                     /gene="frpC"
                     /locus_tag="WIC03_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-regulated protein FrpC"
                     /protein_id="HEZ3884335.1"
                     /translation="MNEGEVVLTPEQIQTLRGYAFRGDTYGGWRYLANLGDRYADDAA
                     AIVGKDANLNGLNLWMKKGVENLWDDTVGKKTRLEKFDRVALQHFSQYVDLINENNGR
                     LPNTSEIERSYYKAVTENGVSSSAAIDLVINRSLPDMADGYWALGLGIEAERIHNEQA
                     VNNPNGSERDNRKQLISALDKGFDGSFKEKHFTFLQSVMMDLTKLGVEYTIDGWQKIG
                     GWGNGIINDLYKSVVKREWTGIFEIVNNNIKQGNEAFKNEINSLVHDMKAAGKEFGDD
                     LNTQWNNLTQAAEIIYNDIVDNTSQGIEKGVKAIKELSEKMKNAASDLADGSAEKAKQ
                     VVEDLAQAAKEAYENAKSTAEKAAQAAREFFKGLPSFKDLAEKFRDLFPNPEGWIDDG
                     HQCFAPWVKETKKRNGKYHVYDPLALDLDGDGIETVATKGFSGSLFDHNRDGIRTATG
                     WVSADDGLLVRDLNGNGIIDNGAELFGDNTKLADGSFAKHGYAALAELDSNGDNIINA
                     ADAAFQTLRVWQDLNQDGISQTNELRTLEELGIQSLDLAYKDVNKNLGNGNTLAQQGS
                     YTKTNGTTAKMGDLLLAADNLHSRFKDKVELTAEQAKAANLAGIGRLRDLREAAALSG
                     DLANMLKAYSAAETKEAQLALLDNLIHKWAETDSNWGKKSPMRLSTDWTQTANEGIAL
                     TPSQVAQLKKNALVSLSDKAKATIDAARDRIAVLDAYTGQDSNTLYYMSEEDALNIVK
                     VTNDTYDHLAKNIYQNLLFQTRLQPYLNQISFKMENDTFTLDFSGLVQAFNHVKETNP
                     QKAFVDLAEMLAYGELRSWYEGRRLMADYVEEAKKAGKFEDYQKVLGQETVALLAKTS
                     GTQADDILQNVGFGHNKNVSLYGNDGNDTLIGGAG"
BASE COUNT          863 a          532 c          630 g          677 t
ORIGIN      
        1 caagtaaaaa ataatttaaa ggatcttatt atgaatgagg gtgaagttgt tttaacacca
       61 gaacaaatcc aaaccttgcg tggttatgct ttccgtggcg atacctatgg cggttggcgt
      121 tatttggcta atttgggtga ccgttatgcg gatgatgctg ctgcaattgt cggtaaggat
      181 gcaaacttaa atggtttgaa tttatggatg aaaaaagggg tggaaaacct atgggatgat
      241 acggtcggta aaaagacccg tttagagaaa tttgatcggg ttgcattgca acatttcagc
      301 caatatgtag atctaattaa tgaaaataat ggtagattac ctaacactag tgaaattgag
      361 agaagttact ataaagccgt taccgaaaat ggtgtttctt ctagtgcagc tattgattta
      421 gttattaatc gttcacttcc ggatatggcg gatggttatt gggcattagg tttggggata
      481 gaagccgaac gtatccacaa tgagcaagca gtaaataatc cgaacggtag cgaaagggat
      541 aatagaaagc agttaatatc tgctttagat aaaggatttg atggatcttt taaagagaag
      601 cattttactt ttttacaatc tgtgatgatg gatctaacaa agttaggtgt tgaatataca
      661 atagatggtt ggcaaaaaat tggaggttgg ggtaatggga taatcaatga tttatataaa
      721 agtgttgtaa aaagagagtg gactggaata tttgagatcg ttaataataa catcaagcaa
      781 ggaaatgaag cttttaaaaa tgaaatcaat agcttggttc atgatatgaa agctgctggc
      841 aaggaatttg gagatgactt aaatacacag tggaataatc tcactcaggc tgccgaaata
      901 atctataatg acatagtaga caatactagt caaggaatag aaaaaggtgt caaagccatt
      961 aaagaattgt ctgaaaaaat gaaaaatgct gcttccgatt tggctgacgg ttcagcagag
     1021 aaagctaaac aagtagtgga agatttggct caagccgcca aagaagcata cgaaaatgcc
     1081 aaatccacag ccgagaaggc tgctcaagca gctcgagaat tttttaaggg cttgcccagt
     1141 tttaaagatc tggccgaaaa atttagagat ctgttcccaa atccggaagg ctggatcgat
     1201 gatggtcacc aatgtttcgc tccttgggtt aaagaaacta aaaaacgcaa tggcaaatat
     1261 catgtctacg acccccttgc cctagatttg gacggcgacg gcatagaaac cgttgccacc
     1321 aaaggctttt caggcagcct atttgaccac aaccgcgatg gcatccgcac cgccaccggt
     1381 tgggtttctg ccgatgacgg tctgcttgtg cgcgatttga acggcaacgg catcatcgac
     1441 aacggtgcgg aactcttcgg cgacaacacc aaactggcag acggctcttt tgccaaacac
     1501 ggctacgcgg ctttggccga attggattca aacggcgaca acatcatcaa cgcggcagac
     1561 gccgcattcc aaaccctgcg tgtatggcag gatctcaacc aggacggcat ttcccaaacc
     1621 aacgaactcc gcacccttga agaattgggt attcaatctt tggatctcgc ctataaagat
     1681 gtaaataaaa atctcggtaa cggtaacact ttggctcagc aaggcagcta caccaaaaca
     1741 aacggtacaa ccgcaaaaat gggggattta cttttagcag ccgacaacct gcacagccgc
     1801 ttcaaagaca aagtggaact cactgccgaa caggcaaaag ccgccaatct tgcgggcatt
     1861 ggtcgtctgc gcgatttgcg cgaagctgcc gcattgtccg gcgatttggc caatatgctg
     1921 aaagcttatt ctgccgccga aactaaagaa gcacagttgg cattgttaga taatttgatt
     1981 cacaaatggg cggaaaccga ttcgaactgg ggcaaaaaat cgccaatgcg actttcaacc
     2041 gattggacgc aaacggctaa tgaaggtatt gcactgacac catcccaagt agcacaacta
     2101 aaaaagaacg ctttagtttc cctttctgat aaagctaaag caactattga cgccgcccgc
     2161 gaccgcattg ccgtgcttga tgcctacacg gggcaggatt ccaacacact ctattacatg
     2221 agcgaggaag atgcgcttaa tatcgtcaaa gtaaccaacg atacatacga ccatctcgcc
     2281 aaaaacatct accaaaacct gttattccaa acccgtttgc agccatattt gaatcaaatc
     2341 agtttcaaaa tggaaaatga tacgttcact ttggatttta gtggtcttgt tcaagcattt
     2401 aaccatgtca aagaaactaa tccgcaaaaa gcttttgtgg atttggccga gatgcttgca
     2461 tatggcgaac ttcgttcttg gtatgaaggc cgaagactaa tggccgatta tgtggaggag
     2521 gcaaaaaaag caggtaaatt tgaagattac cagaaagtgt tgggtcagga gaccgttgca
     2581 ttattagcta aaacatcggg tacgcaagca gatgatatcc tgcaaaatgt aggctttggt
     2641 cataataaaa atgtttcttt atatggtaat gacggcaacg acactctgat cggcggtgca
     2701 gg
//
LOCUS       DAWHSK010000242         2690 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.242, whole genome shotgun sequence.
ACCESSION   DAWHSK010000242 DAWHSK010000000
VERSION     DAWHSK010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.242"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..864)
                     /locus_tag="WIC03_001880"
     CDS             complement(<1..864)
                     /locus_tag="WIC03_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ3884336.1"
                     /translation="MTDITPFANRLGKNIKHLMKWVKRNGIEAWRIYDRDIPQFPFAA
                     DVYGDRIHLQEYDTGWLMRPEEYEAWLAEVLEAVAFVTGFAPEQIRLKRRERQKGLQQ
                     YEKTGKAGDDFVITENGRKFWVNLDKYLDTGLFLDHRNTRKKVGETAAGKRFLNLFSY
                     TGSFTVYAATGGAASSETVDLSNTYLDWAKRNFELNGIDTERHKIVRADVFQYLQTAY
                     GEGRRFDLIVMDPPSFSNSKKMLDILDIQRDHKKLIDGAVKLLASDGILYFSNNLRSF
                     VLDDLVSEQYAV"
     gene            1192..2580
                     /locus_tag="WIC03_001881"
     CDS             1192..2580
                     /locus_tag="WIC03_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEZ3884337.1"
                     /translation="MSIKFADLNLDKNILSAVSSEGYESPTPIQAQAIPFALEGRDIM
                     ASAQTGSGKTAAFLLPTLQKLTKRSEKPGKGPRALVLTPTRELAAQVEKNALAYAKNM
                     RWFRTVSIVGGASFGYQTRALSKPVDLIVATPGRLMDLMQSGKVDFERLEVLILDEAD
                     RMLDMGFIDDIETIVEATPSDRQTLLFSATWDGAVGKLARKLTKDPEIIEVERVDDQG
                     KIEEQLLYCDDMRHKNRLLDHILRDANIDQCVIFTSTKAMTEVIADELYEKGFAANCL
                     HGDMPQGWRNRTLMDLRKGRCKILVATDVAARGIDVPTITHVINYDLPKQAEDYVHRI
                     GRTGRAGRTGIAITFAEVNEYVKVHKIEKYINRKLPELTIEGMEPTRKRKSAGGKPKG
                     KGGWGDRKSGGWRGDHKPSKEGFGGKTRGEGFKKEGFKRDGFKKTGEGFKGKRKAGDS
                     FAGKGERRYKDR"
BASE COUNT          655 a          725 c          711 g          599 t
ORIGIN      
        1 gacggcgtat tgttccgata ccaagtcgtc taagacgaag ctgcgcaggt tgttggaaaa
       61 gtacaaaatg ccgtctgaag cgagcagctt caccgcgccg tcaatcagct ttttgtggtc
      121 gcgctggatg tcgagaatgt cgagcatctt tttgctgttg gagaagctgg gcgggtccat
      181 cacaatgagg tcgaaccgcc tgccttcccc atatgccgtc tgaagatatt gaaacacgtc
      241 ggcgcggacg attttgtgtc gttccgtatc gatgccgttc aattcaaaat tgcgtttcgc
      301 ccaatcaaga tatgtgttgg acaaatcgac ggtttcgctg gatgccgcgc cgccggtggc
      361 ggcatagacg gtgaagctgc cggtgtagga aaacaggttt aaaaaacgtt tgcccgccgc
      421 cgtttcgccg acttttttgc gcgtgtttcg atgatccaaa aaaagccccg tatccaaata
      481 tttatcaagg ttgacccaaa acttgcggcc gttttcggtg atgacgaaat cgtcgcccgc
      541 cttgccggtt ttctcgtact gctgcaaacc tttttggcgt tcgcggcgtt tgaggcggat
      601 ttgttcgggc gcaaaaccgg taacgaaagc gacggcttcc aagacttcgg caagccacgc
      661 ttcgtattct tcgggccgca tcagccagcc ggtatcgtat tcctgaaggt ggattcgatc
      721 gccgtaaaca tcggcggcaa aggggaattg ggggatgtcg cggtcgtaaa tgcgccaggc
      781 ttcgatgccg ttgcgtttca cccatttcat aaggtgtttg atgtttttgc ccaagcggtt
      841 ggcaaacggt gtgatgtcgg tcattggttt caggcggaat aaagtggaaa acggcaattt
      901 tactgtaatt aacgcccgat tgcttgaccg tttcgggcaa accctatacc atccgtcgct
      961 tatcttgtca tacgaagcca tcgccttcca acctaaaccg cccttacggg cgcgtttctt
     1021 ctgttgcttt gattttgcaa agcatatctg tgcaggttgc cgtcgatgta aaccacaagc
     1081 aagccgcttg cgacaaccct gtaacttcac attccccgta tcgttaccct tccctgcttc
     1141 aggccgtctg aacctttcgg acgcgggcgt tgttgtcttc caaggatagc catgtctatt
     1201 aaatttgccg atttgaacct tgataaaaac attttgtccg ccgtcagcag cgagggttac
     1261 gaaagcccga cgccgattca ggcgcaagcc attccgtttg ctttggaagg ccgcgacatc
     1321 atggcttcgg cgcaaaccgg ctccggcaaa accgccgcct ttctgttacc gactttgcaa
     1381 aaactgacca aacgcagcga aaaaccgggc aaaggcccgc gtgctttggt gttgaccccg
     1441 acccgcgaac tggcggctca agtcgagaaa aacgcgctgg cgtatgccaa aaatatgcgt
     1501 tggttccgca ccgtcagcat cgtcggcggc gcgtctttcg gctaccaaac ccgtgccctg
     1561 agcaaaccgg tcgatctgat tgtcgccacg ccgggccgtc tgatggacct gatgcaaagc
     1621 ggcaaagttg attttgaacg tttggaagtg ctgattttgg acgaagccga ccgtatgttg
     1681 gatatgggct ttatcgacga catcgaaacc atcgtggaag caacgccgag cgaccgtcag
     1741 actttgttgt tctccgccac ttgggacggc gcggtcggca aactggcgcg caaactgacc
     1801 aaagaccctg aaatcatcga agtcgaacgc gtggacgatc aaggcaaaat cgaagaacaa
     1861 ctgctgtact gcgacgatat gcgccacaaa aaccgcctgc tcgatcatat cttgcgcgat
     1921 gccaatatcg atcaatgcgt gattttcacg tccaccaaag ccatgaccga agtcattgcg
     1981 gatgaactgt acgaaaaagg tttcgccgca aactgcctgc acggcgatat gccgcaaggc
     2041 tggcgcaacc gcacgctgat ggatttgcgt aaaggccgct gcaaaatttt ggttgccacc
     2101 gatgttgccg cacgcggtat cgacgtaccg accattaccc acgttatcaa ctacgacctg
     2161 ccgaaacagg cggaagacta cgtccaccgc atcgggcgca ccggccgcgc aggccgcacg
     2221 ggtattgcga ttacgtttgc cgaagtgaac gaatacgtca aagtccacaa aatcgaaaaa
     2281 tacattaacc gaaaactgcc cgaactgacc atcgaaggca tggaaccgac ccgcaaacgc
     2341 aaatccgcag gcggcaagcc gaaaggcaaa ggcggctggg gcgatcgtaa atccggcggt
     2401 tggcgcggcg atcataaacc gagcaaagaa ggcttcggcg gcaaaacgcg cggcgaaggt
     2461 ttcaagaaag aaggctttaa gagagacggt ttcaaaaaaa ccggcgaagg cttcaaaggc
     2521 aaacgcaaag ccggcgattc ttttgcaggc aaaggcgaac gccgttacaa agaccgctaa
     2581 gccccaacct gccgcataaa ccaatgccgt ctgaaaccga tttcgagttt cagacggcat
     2641 ttttgcaatg tttcagcacc gcccggcttt gatacccaaa ggattaatct
//
LOCUS       DAWHSK010000243         2678 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.243, whole genome shotgun sequence.
ACCESSION   DAWHSK010000243 DAWHSK010000000
VERSION     DAWHSK010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.243"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..268
                     /locus_tag="WIC03_001882"
     CDS             <1..268
                     /locus_tag="WIC03_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEZ3884338.1"
                     /translation="PRVMLFDEPTSALDPEMVGEVLKVMKDLAESGMTMMCVTHEMGF
                     AREVADRVLFVDKGQILEDETPEAFFTNPKHERAKQFLQQVMTH"
     gene            341..700
                     /locus_tag="WIC03_001883"
     CDS             341..700
                     /locus_tag="WIC03_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884339.1"
                     /translation="MYFVDRTAVVLKPTVRFLEWLKSTDENMPDLTIEQLRANCSVFL
                     VPQFDEPEAVVSYFDERYRQIFEAELAGWDIDKDKWPQDMGLKAFWEFFDIEIHDMVL
                     DMEEAELNITPVFDNMM"
     gene            705..1145
                     /locus_tag="WIC03_001884"
     CDS             705..1145
                     /locus_tag="WIC03_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FolI"
                     /protein_id="HEZ3884340.1"
                     /translation="MRAFQTALRPSRILNILTVSLHAASLAVCLTWFYGWMMWFGLAA
                     LVASYAYSLRITNLKHRHAITAITIDRDGQAEIVSGKDKTAATLAGSSMVTPYALFLQ
                     WDTGGTTVSQCIIPDMTDKESYRRLKVWILWRQPKKTAETDTPS"
     gene            1177..2451
                     /gene="folC"
                     /locus_tag="WIC03_001885"
     CDS             1177..2451
                     /gene="folC"
                     /locus_tag="WIC03_001885"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEZ3884341.1"
                     /translation="MKTLQDWLSHLETAHSGGLIDMGLERVGEVKKRMKLTPQCPVVV
                     VAGTNGKGSVCAYLTQIYKQAGYKTGTLTSPHLLRYNERIAINAEPVSDDTIIASFER
                     IEAAHGEISLTYFEFNTLAAVDIFMREQVDVMILEVGLGGRLDAVNVFDCDCAVVTSV
                     DLDHQAFLGDTVEQVGFEKAGVFRGGKPAICGQNPAPASLVAHAEAIGAKLLMVQRDF
                     EFHAMENIQWNYRFRPQHSDGPARNRNALPFPALRGAYQLSNAACALTVLECLNDKLP
                     VDIGAIKRGLLLVENPGRFQVLPGRPLTVLDVGHNPHAARALRRSLINLAFAQKRTAV
                     FSMLSDKDIDGVLEIVKDQFDEWYIAPLDVPRGMTLESLQNKLHEHGIENIQTFAAVR
                     EAYRAAASKAGENDRIVVFGSFHTVADVMSVL"
     gene            2464..>2678
                     /locus_tag="WIC03_001886"
     CDS             2464..>2678
                     /locus_tag="WIC03_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HEZ3884342.1"
                     /translation="MSENKQNEVLSGYEQLKRRNRRRLVTASCLVAASCILLAAALSS
                     GPAEQTAGETSGVENKAAGAAQTPALKS"
BASE COUNT          664 a          766 c          710 g          538 t
ORIGIN      
        1 accgcgcgta atgttgtttg acgaaccgac ctccgcactc gaccccgaaa tggtcggcga
       61 agtgttgaaa gtgatgaaag acttggcgga aagcggtatg acgatgatgt gcgtaaccca
      121 cgaaatgggt tttgcccgcg aagttgccga ccgagtgctt tttgtcgata aagggcaaat
      181 cctcgaagac gaaacgcccg aagcgttttt taccaacccg aaacacgaac gcgccaaaca
      241 attcctgcaa caggtcatga cccactgatg ctatgccgtc tgaaaaccga acgttcagac
      301 ggcatagccc gacaccgcaa gatgattgag aaagaaaacc atgtattttg ttgaccgcac
      361 cgccgtcgtc ctcaagccga ccgtccgctt tttggaatgg ctcaaaagca ccgacgaaaa
      421 tatgcccgac ctgaccatag aacagcttcg tgccaactgt tccgtcttcc tcgttccgca
      481 gttcgacgaa ccggaagccg tcgtttccta tttcgacgaa cgctaccgcc agattttcga
      541 ggcggagctg gcaggttggg acatcgataa ggataaatgg ccgcaagata tggggctgaa
      601 ggcgttttgg gaatttttcg acatcgaaat ccacgatatg gtgttggaca tggaagaagc
      661 ggaattgaac attacccccg tgttcgacaa catgatgtaa ggctgtgcgc gcctttcaga
      721 cggcattgag gccgtcccgc atcctgaaca tcctgactgt atcgcttcac gccgcctccc
      781 ttgccgtctg cctgacttgg ttttacggct ggatgatgtg gttcggtttg gcggctttgg
      841 tggcaagtta cgcatactcg ctcaggataa cgaatctgaa acaccgccat gccataaccg
      901 ccattaccat agaccgcgac ggacaggcgg aaatcgtatc cggcaaagac aaaacagcgg
      961 caacacttgc gggcagcagt atggttacgc cttatgcctt attcttacaa tgggacacag
     1021 gcggcacaac cgtcagccaa tgcatcatac ccgacatgac ggacaaagaa tcctaccggc
     1081 ggctgaaagt ctggatactt tggcggcagc cgaaaaaaac cgccgaaacc gatacaccaa
     1141 gctgatttca gacggcattt gaacacggaa cactcaatga aaacactgca agactggctc
     1201 tcgcatttgg aaaccgccca cagcggcggc ttgatcgata tgggcttgga acgcgtaggc
     1261 gaagtgaaaa aacggatgaa gctcacgccg caatgccccg tcgtcgttgt cgcgggaaca
     1321 aacggcaaag gttcggtctg cgcctacctg acacagattt acaaacaggc gggctacaaa
     1381 accggcacac tgaccagccc gcatttattg cgttacaacg aacgcatcgc catcaacgcc
     1441 gaacccgttt cggacgatac gattattgcc tctttcgagc gcatcgaagc ggcgcacggc
     1501 gaaatatcgc tgacttattt tgaattcaat acgttggcgg cggtcgacat tttcatgcgc
     1561 gaacaggttg acgtgatgat attggaagtc ggcttgggcg gacgcttgga cgcggtcaac
     1621 gtgttcgact gcgattgcgc ggtggttacc agcgtggatt tggaccatca ggcatttttg
     1681 ggcgatacgg tcgagcaggt cggctttgaa aaggcaggcg tgttccgcgg cggcaaaccc
     1741 gccatctgcg gtcaaaaccc cgcgcccgca tcattggtcg cacacgccga agccataggc
     1801 gcgaaactgc tgatggtgca gcgcgatttc gagtttcacg cgatggaaaa catccaatgg
     1861 aactaccgct tccggccgca gcattcagac ggccccgcac gcaaccgcaa tgccctgccc
     1921 ttccccgcat tgcgcggcgc ataccagctt tccaacgccg cctgcgcgct gaccgtgttg
     1981 gaatgcttga acgacaaatt gcctgtggac atcggcgcca tcaaacgcgg tttgttgctg
     2041 gttgaaaacc ccggccgctt ccaagtcctg cccggccgtc cgctgaccgt tttggatgtc
     2101 ggacacaatc cccacgctgc ccgcgccctg cgccgcagcc tgattaattt ggcgtttgcg
     2161 caaaaacgca ccgccgtgtt cagcatgttg tccgacaaag acatagacgg cgtgttggaa
     2221 atcgttaaag accagtttga cgagtggtat atcgcaccgt tggacgtacc gcgcggcatg
     2281 acgctcgaga gcctgcaaaa caaactgcac gaacacggca tcgaaaacat acagacattc
     2341 gccgccgtcc gcgaagcata ccgggcggca gcctcaaaag cgggcgagaa cgacagaatc
     2401 gtcgttttcg gctcatttca taccgttgcc gatgtcatgt cggtacttta aaaggaaact
     2461 tctatgtccg aaaacaaaca aaacgaagtc ctgagcggtt acgaacaact caaacggcgc
     2521 aaccgccgcc gcctcgtaac ggcaagttgc ctggttgccg cctcctgcat cctgctggca
     2581 gccgccctca gttccggccc tgccgaacag actgccggcg aaacaagcgg cgtagaaaac
     2641 aaagcggcag gtgcggcaca aacccctgcc ttgaaatc
//
LOCUS       DAWHSK010000244         2678 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.244, whole genome shotgun sequence.
ACCESSION   DAWHSK010000244 DAWHSK010000000
VERSION     DAWHSK010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.244"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(36..1106)
                     /locus_tag="WIC03_001887"
     CDS             complement(36..1106)
                     /locus_tag="WIC03_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEZ3884343.1"
                     /translation="MYRRKGWGIKPWMGAGAAFAALVWLIFALGDTLTPFAVAAVLAY
                     VLDPLVEWLQKKGLNRASASMSVMVFSLILLLALLLIIVPMLVGQFNNLASRLPQLIS
                     FMQNTLLPWLKNTIGGYMEIDQASIIAWLQAHTGELSNALKAWFPVLMRQGGNIVSSI
                     GNLLLLPLLLYYFLLDWQRWSCGIAKLVPRRFAGAYTRITGNLNEVLGEFLRGQLLVM
                     LIMGLVYGLGLVLVGLDSGFAIGMVAGILVFVPYLGAFTGLLLATVAALLQFGSWNGI
                     LSVWAVFAVGQFLESFFITPKIVGDRIGLSPFWVIFSLMAFGQLMGFVGMLAGLPLAA
                     VTLVLLREGVQKYFAGSFYRGR"
     gene            1291..1566
                     /locus_tag="WIC03_001888"
     CDS             1291..1566
                     /locus_tag="WIC03_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HEZ3884344.1"
                     /translation="MTEQENKTSLIEFPCTFPLKVMGAVHPEFEQAVLDTVRLHAPDT
                     QAHHITTRPSSKGNYTGATVQVKVENQEQLDNIYRALTSHELVKVVL"
     gene            1568..2191
                     /gene="lipB"
                     /locus_tag="WIC03_001889"
     CDS             1568..2191
                     /gene="lipB"
                     /locus_tag="WIC03_001889"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEZ3884345.1"
                     /translation="MKIIHKGLVEYLPTFEAMKTFNAGRNADTEDELWVVEHPPVFTQ
                     GLVGKPEHLLIRDDIPVVQIDRGGQITYHGPGQLVVYTMIDFKRRKTSVRNIVSALEN
                     SIIATLAEYGIEAAADPKRPGVYVGERKIASLGLRIKNGSVYHGLALNVNMDLSPFTH
                     INPCGYAGMEMTQIADFVQPCPTPDEVAAKLTAHLETQFTPKADNNE"
     gene            2184..>2678
                     /locus_tag="WIC03_001890"
     CDS             2184..>2678
                     /locus_tag="WIC03_001890"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEZ3884346.1"
                     /translation="MSEIKTDDPKRGIKLRGADKTARIPIKVVPLQEKLKKPEWIRAK
                     LPSRKFFEIKDILREQKMHTVCEEASCPNIGECFSKGTATFMIMGDICTRRCPFCDVG
                     HGRPNMLDPDEPKNLAESVKAMNLRYVVITSVDRDDLRDGGAQHFADCIKAIRETSPN
                     TKIEI"
BASE COUNT          795 a          849 c          584 g          450 t
ORIGIN      
        1 ggttgtatcc cgcttcaaat atgtttcgga accgcctacc tgccccggta aaaactgccg
       61 gcaaaatatt tctgcacgcc ctcgcgaagc aagaccaagg ttacggcggc caaaggcaat
      121 cctgccaaca ttccgacgaa acccatcagc tgtccgaacg ccatcaggga aaagataacc
      181 caaaacggcg acagcccgat acggtctccc acgattttcg gcgtaatgaa aaaactttcg
      241 agaaactgtc ctacggcaaa aaccgcccaa accgatagga tgccgttcca cgaaccgaac
      301 tggagcaagg cggcgacggt tgccagcagc agtcctgtaa acgcgcccaa atagggaaca
      361 aaaaccaaaa taccggcaac cataccgatt gcaaaccccg aatccagccc gaccagcacc
      421 aaccccaagc cgtaaaccaa acccataatc agcatcacca gaagctgccc gcgcaaaaat
      481 tcgcccaata cctcgttcaa attacccgta atgcgcgtat aagcaccggc aaaacgcctc
      541 ggaaccagtt tggcgatgcc gcacgaccac cgctgccaat ccagcaggaa ataataaagc
      601 agcaagggca gcagcagtag gttgccgata ctgctgacaa tattgccgcc ctgcctcatc
      661 aaaacgggaa accacgcctt aagcgcgttg ctcaactcgc ccgtatgcgc ctgaagccac
      721 gcaataatag atgcctgatc gatttccata tatccgccga ttgtattttt caaccacggc
      781 agcagcgtgt tctgcataaa actgatcaat tggggtaggc gcgatgccaa attattgaac
      841 tgcccgacca gcatagggac aataatcaac aataatgcca acaacaaaat caaggaaaac
      901 accatcacag acatcgaagc ggatgcacgg ttcaaaccct ttttctgcaa ccattcgacc
      961 aaagggtcca acacatacgc cagcaccgcc gcaaccgcaa acggagtcaa agtatcgccg
     1021 agcgcgaaaa tcagccaaac caaggcggca aacgccgcac cggcacccat ccacggcttg
     1081 atgccccacc ctttcctccg atacataaaa accacccttc taaaaataat attgcgcgcc
     1141 agtatagcag aaccgccgcg ccgtcaaaaa agcctccgcc ttcccgatag cctgttatcg
     1201 cggtttaggc taaaataacg cacatccgat gccgtctgaa aggcaaaccc gccttcagac
     1261 ggcataccgt acacaacaag gcagcacatc atgaccgaac aagaaaacaa aacatccctc
     1321 atcgaatttc cctgcacctt cccattaaaa gtaatggggg cggttcatcc cgagttcgag
     1381 caggcggtat tggacaccgt ccgcctccac gcccccgaca cgcaggcgca ccacatcacc
     1441 acgcgtccga gcagcaaagg caactatacc ggcgccaccg ttcaggtaaa ggttgaaaac
     1501 caagaacaat tggacaacat ctaccgcgcg ctgacttcgc acgaactggt caaagtggta
     1561 ctttgagatg aaaatcatac acaaaggttt ggtcgaatat ctgccgactt ttgaagcgat
     1621 gaaaaccttt aatgccggac ggaatgctga caccgaagac gaactgtggg tcgtcgaaca
     1681 cccgcccgtg ttcacacaag gactggtggg aaaacccgaa cacctgctga ttcgcgacga
     1741 cattcccgtc gtccaaatcg accggggcgg gcagattacc tatcacgggc ccgggcaatt
     1801 ggtcgtttat acgatgattg atttcaaacg gcgcaaaacc agcgtccgca acatcgtttc
     1861 cgcgcttgaa aacagcatca tcgccacatt ggcagaatac ggcatcgaag cggcggcaga
     1921 ccccaaacgc cccggcgttt atgtcggaga acgcaaaatc gcctcactgg ggctgcgtat
     1981 caaaaacggc tccgtctatc acgggcttgc gctcaacgtc aatatggatt taagcccgtt
     2041 tacccacatc aacccctgcg gctacgccgg tatggaaatg acgcaaatcg cggattttgt
     2101 ccaaccctgc cccacgccgg acgaagtcgc cgccaaactt accgcacacc ttgaaacaca
     2161 attcacaccg aaagcagaca acaatgagtg aaatcaaaac cgacgacccc aaacgcggca
     2221 tcaaactcag aggcgcggac aaaaccgccc gcatccccat caaagtcgtc ccccttcagg
     2281 aaaaattaaa aaagcccgaa tggatacgcg ccaaacttcc atcgcgcaaa ttctttgaaa
     2341 tcaaagacat tttgcgcgaa caaaaaatgc acaccgtttg cgaggaagcc tcctgcccca
     2401 acatcggcga atgcttcagc aaaggcacgg cgaccttcat gattatgggc gacatctgca
     2461 cccgccgctg cccgttctgc gacgtgggac acggccgccc gaatatgctc gaccccgacg
     2521 aaccgaaaaa cctcgccgaa tccgtcaaag ccatgaacct gcgttacgtc gtcatcacct
     2581 ccgtcgaccg cgacgacctg cgcgacggcg gcgcacagca tttcgccgac tgcatcaaag
     2641 ccatccgcga aaccagcccg aacaccaaaa tcgaaatc
//
LOCUS       DAWHSK010000245         2657 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.245, whole genome shotgun sequence.
ACCESSION   DAWHSK010000245 DAWHSK010000000
VERSION     DAWHSK010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.245"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(64..1434)
                     /gene="ffh"
                     /locus_tag="WIC03_001891"
     CDS             complement(64..1434)
                     /gene="ffh"
                     /locus_tag="WIC03_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEZ3884347.1"
                     /translation="MLDNLTSRFSNVLKNIRGQAKLTEDNIKEALREVRLALLEADVA
                     LPVVKEFVNNVKEKALGQEVAGSLTPDQAFIGVVNQALVELMGKENKTLDLSVAPPAV
                     VLMAGLQGAGKTTTVGKLARLLKNDQKKKVLVVSADVYRPAAIEQLRLLAEQVGVDFF
                     PSDTNQKPVEIATAAVDYAKKHFYDVLMVDTAGRLAIDEEMMNEIKALHAAVNPVETL
                     FVIDAMLGQDAVNTAQAFNEALPLTGVVLTKMDGDSRGGAALSVRHVTGKPIKFIGVG
                     EKINGLEPFHPDRLASRILGMGDVLTLIEDVQKGIDEEAAAKMAKKLHKGKGFDLNDF
                     KEQIQQMRNMGGLENLMSKMPGELGQISKQIPEGTAEKAMGKVEAIINSMTPKERANP
                     ALLKASRKRRIAVGAGTTVQEVNKLLKQFEQMQQMMKMFSGNGLGKLMRMAKGMRGIK
                     GMFPGL"
     gene            1654..2460
                     /locus_tag="WIC03_001892"
     CDS             1654..2460
                     /locus_tag="WIC03_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HEZ3884348.1"
                     /translation="MPTVFIFLMAVYAGLGAFAWHCQQQGRGRDYPWKTELPVLGAAL
                     TVHGAALLMPVIQDKIIIMGFGYSGSLIVWMMLFIYFAGSFFYPLRGVQLLLYPCAVL
                     MLLSGLVFPGKFSGYEITDLPFMLHIGTSLLAYGLFGIATLLSVLTLLLNRSLHRRNF
                     SKLAGFLPSLLSLEKLMFQAMWAGFILLTYSVVSGTFFAEAVFGKPMTFTHKTVFGIL
                     SWLIYGGLLLKHSMTAWRGKKAAVWTIIGFVSLMIAYMGSKFVLEIILKR"
BASE COUNT          583 a          685 c          671 g          718 t
ORIGIN      
        1 atcagaatat aaaacaaaaa cgccgtctga aacttttcag acggcgtttt ctgttaaatc
       61 ggcttacaaa cccgggaaca tcccttttat ccccctcatt cctttcgcca tacgcatcag
      121 tttgcccaag ccgttgccgc tgaacatctt catcatctgt tgcatttgtt caaactgttt
      181 gagcaatttg ttcacttcct gtaccgtcgt gcccgcgccc actgcgatac ggcgtttgcg
      241 gctggcttta agcagggcag ggttggcgcg ttcttttgga gtcatcgagt tgatgatggc
      301 ttccactttg cccatcgctt tttcagccgt gccttcgggg atttgtttcg agatttgacc
      361 cagttcgccc ggcattttcg acatcaggtt ttccaaaccg cccatattgc gcatttgctg
      421 gatttgttct ttaaagtcgt tgaggtcgaa gcctttgcct ttgtgtagct ttttcgccat
      481 tttggcggcg gcttcttcat caataccttt ttggacgtct tcaatcaggg tcaatacgtc
      541 gcccatgccg aggatacggc tggcaagacg gtcggggtgg aaaggttcga ggccgttgat
      601 tttttcgccg acaccgataa atttaatcgg cttgccggtt acgtggcgca cggacaaagc
      661 tgcgccgccg cgcgaatcgc cgtccatctt ggtcaatacg acaccggtca gcggcagggc
      721 ttcattaaat gcctgagcag tgttcaccgc atcctgaccc agcatcgcat cgatgacgaa
      781 caaagtttcc accgggttaa ccgccgcatg aagggctttg atttcgttca tcatctcctc
      841 atcgattgcc aaacggccgg cggtatcgac catcaataca tcgtaaaaat gttttttggc
      901 gtaatcgacg gcggcagttg caatttcaac cggtttttgg ttggtatcgg atgggaaaaa
      961 gtccacgccg acctgttcgg ccaacagacg cagctgttca atcgcggcag ggcggtaaac
     1021 gtcggcggat accaccaaaa cttttttctt ctgatcgttt ttcagcaggc gggcgagttt
     1081 gccgacagtc gtcgtcttac ctgcgccctg caaaccggcc atcaaaacga cggcgggcgg
     1141 cgcaaccgat aaatccagcg ttttgttttc cttgcccatc agttcgacta aggcttggtt
     1201 gaccacgccg ataaatgcct gatccggcgt caggctgccc gccacttcct gaccgagggc
     1261 cttttctttg acgttgttga cgaactcttt gacgacaggc agggcgacat ccgcctcaag
     1321 cagggcgagt cggacttcgc gcaaggcttc tttaatgttg tcttcggtca gtttggcttg
     1381 cccgcggatg tttttgagga cattgctgaa gcggctggtt aaattgtcta acatactggt
     1441 ccttggtctg aataagaata gcttgcccca tcaggggcat tctttgttaa aataaaatca
     1501 aaataatttg atgcggcttg tgtgccggac agcatatcgg caaatccgtc aaggcttgcc
     1561 cgaaacgggg attttacaat tccaacgtta aaagttccaa tatttcataa gcggccgcat
     1621 acggcgcaac agtatagata gagaaagtcc accatgccga cagttttcat ctttttgatg
     1681 gcagtttacg caggattggg tgcatttgca tggcactgcc aacagcaggg gcgcggccgg
     1741 gattacccgt ggaagacgga attgccggtt ttgggcgcgg cattgaccgt ccacggcgcg
     1801 gcactgctta tgccggtcat tcaagacaaa atcatcatta tgggcttcgg gtattccggc
     1861 agcctgattg tttggatgat gctgtttatt tattttgccg gcagcttctt ttatccgctg
     1921 cgcggagtgc agttgctgct gtatccttgc gccgtactga tgctgctgtc aggtttggtt
     1981 tttcctggaa aattctcggg atatgaaatt accgaccttc cctttatgct gcatatcgga
     2041 acttcgctgc tcgcatacgg gctgttcggc atcgcaacat tattgtccgt tttgaccctg
     2101 ctgctgaatc ggagcctgca ccgcaggaac ttctccaagc tcgcaggatt cctgccgtcg
     2161 ctgctcagtt tggaaaaact catgttccag gccatgtggg caggtttcat cctgctgacc
     2221 tattccgtcg tcagcggaac attttttgcc gaagccgtat tcggcaaacc catgaccttt
     2281 acccataaaa ccgtattcgg catattgtca tggctgattt acggcggact gctgctcaag
     2341 cacagcatga ccgcatggcg cggcaaaaaa gccgccgtgt ggaccatcat cggatttgtc
     2401 agccttatga ttgcctatat gggcagcaag ttcgtattgg aaatcattct gaaaagataa
     2461 gaagagccaa cagatgccgt atgaatcccc aagtttcaga cggcatattc acaaaggcgc
     2521 accggtcaga ggagggggag gaagggattg ttggaggcgg cgcagcattt agcagaaata
     2581 aaaaacctta tccgacagcg acatgacgaa tttccccaaa aaaatcccac tgaaagcatt
     2641 gaccgttttt ccctgtg
//
LOCUS       DAWHSK010000246         2631 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.246, whole genome shotgun sequence.
ACCESSION   DAWHSK010000246 DAWHSK010000000
VERSION     DAWHSK010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.246"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            131..973
                     /locus_tag="WIC03_001893"
     CDS             131..973
                     /locus_tag="WIC03_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HEZ3884349.1"
                     /translation="MCQLLGMNCNTPTDIMFSFEGFRRRGGITDHHADGFGIGFFEGK
                     GVRLFHDDKPSANSPVADLVRAYQIKSENVVAHIRKASQGQTSLANTHPFMREMWGGY
                     WLFAHNGHLIDFFPEQGEFFHPVGTTDSERAFCHILNRLRTRFAARPDDDTLFDAIAG
                     LTHEIRKFGLFNFMLSDGIALFAHASTLLHYIVRQAPFGKARLLDDDVMVDFAEVTTP
                     SDRVAVIATLPLTRDESWSQLAVDELVMFREGNIVRHDRPENPVYMSVEEGLETARAA
                     GVAV"
     gene            1003..1467
                     /gene="nrdR"
                     /locus_tag="WIC03_001894"
     CDS             1003..1467
                     /gene="nrdR"
                     /locus_tag="WIC03_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEZ3884350.1"
                     /translation="MKCPFCAHPDTRVADSRLMDERNAVRRRRYCPNCGKRFGTLETA
                     ELKMPAVIGPDKKRSPFNAQRLRNDLTAAARKSALTPEQIDETVRLTEHRLYTSGQRD
                     IPSAALADMVLKELLRQDTEAAVRFAALHKRFDNPADFASWLAQAVKTDRKA"
     gene            1499..2608
                     /gene="ribD"
                     /locus_tag="WIC03_001895"
     CDS             1499..2608
                     /gene="ribD"
                     /locus_tag="WIC03_001895"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEZ3884351.1"
                     /translation="MFSETDISMMQNALKLAALGRFSTSPNPRVGCVIAQGSQIVGQG
                     FHVKAGEPHAEVHALRQAGEMAQGATAFVTLEPCSHYGRTPPCAEALLRSGVTRVVAA
                     MRDPNPLVAGKGLALLEAAGIKTECGLLEHQARELNRGFLSRIERRRPFVRLKCAVSL
                     DGKTALSDGSSFWITGEDARADVQVLRAESCAVLTGIGTVLADNPRLNVRAFPTLRQP
                     ARIVLDSRLRLPLDSHLLTDGQSPTYIATLERDEDKLRPYRKHAHVRILMPSETAGGK
                     IDLNSLMRLLADEGIGELIVEAGSELTSAFLAEDLADEIVLYRSPKILGSGKDLFSLP
                     ENRAALSAPPLWMPVSTEILGHDIKTVFRKNDNAF"
BASE COUNT          572 a          888 c          655 g          516 t
ORIGIN      
        1 gaaccttaag tttccgtctt tgttttctgt tttcgcggga ataatgggga acaaaccgta
       61 tttcagacgg cattttcagt tcggggtata atccgaatac ttgcgaccat ctgaatcatt
      121 gggacaaact atgtgtcaac tgctgggcat gaactgcaat acgccgaccg atattatgtt
      181 ttcctttgaa ggcttccgcc ggcgcggcgg cattaccgac catcatgccg acggtttcgg
      241 catcggcttt ttcgaaggca aaggcgtgcg cctgttccat gacgacaaac ccagtgccaa
      301 ttcgcccgtc gccgacctcg tgcgcgccta ccagattaaa tcggaaaacg tcgtcgccca
      361 tatccgcaaa gcatcgcaag gacaaacctc gctggcgaac acccatccct ttatgcgcga
      421 aatgtggggt ggctactggc tgtttgccca caacgggcat ttgattgatt ttttccccga
      481 acagggcgaa tttttccacc ccgtcggcac aaccgattcc gaacgcgcgt tctgccacat
      541 cctcaaccgc ctgcgcaccc gctttgccgc ccgtcccgac gacgacacgc tgtttgacgc
      601 gattgcgggg ctgacgcacg aaatccgcaa gttcgggctg tttaacttta tgctttcaga
      661 cggcattgcc ctgtttgccc acgccagcac actgctgcac tacatcgtcc gccaagcccc
      721 gttcggcaag gcgcgcctgc tcgacgacga cgtgatggtc gattttgccg aagtaaccac
      781 gccctccgac cgcgtcgccg ttatcgccac cctgccgctg acccgcgacg aatcatggtc
      841 ccaacttgcc gtggacgaac tggtcatgtt ccgcgaaggc aacatcgtcc gacacgaccg
      901 tcccgaaaac cccgtttata tgagtgtcga agaaggtttg gaaaccgcgc gcgccgccgg
      961 cgtcgccgtc tgaacttcag acgacatagg aggacgaacc cgatgaaatg cccgttttgc
     1021 gcccaccccg acacccgcgt tgccgattcg cgcctgatgg acgaacgcaa cgccgtccgc
     1081 cgccgccgat actgccccaa ctgcggcaaa cgcttcggca cgctcgaaac cgccgaactc
     1141 aaaatgcccg ccgtcatcgg tccggacaaa aaacgttcgc cctttaatgc acaacgcctc
     1201 cgcaacgacc tgaccgccgc cgcccgaaaa tccgccctga cacccgaaca gatcgacgaa
     1261 accgtccgcc tgacggaaca caggctctac acttcgggtc agcgcgacat cccctctgcc
     1321 gcacttgccg acatggtgct aaaagaactg ctccgacaag atacggaagc cgccgtccgt
     1381 ttcgccgccc tgcacaaacg cttcgacaat ccggcagact ttgcctcgtg gctggcgcaa
     1441 gccgtcaaaa cagaccgcaa agcctgattc ccccaaccca tactgatacg gtatccccat
     1501 gttttccgaa acagatattt ctatgatgca aaacgccctc aaacttgccg ctttggggcg
     1561 tttttccact tcgcccaatc cgcgcgtcgg ctgcgttatc gcacaaggca gccaaattgt
     1621 cggacagggc ttccacgtca aagcgggcga accgcacgcc gaagtccacg ccctgcgtca
     1681 ggcgggcgaa atggcacaag gcgcgaccgc ctttgttacc ctcgaaccgt gcagccatta
     1741 cggacgcaca ccgccctgcg ccgaagctct gctccgttcc ggcgtaacac gcgtcgttgc
     1801 cgccatgcgc gaccccaacc cgctggttgc aggcaaaggg cttgccctgc tcgaagcagc
     1861 aggcatcaag acggaatgcg gtttactcga acatcaggca agggaactca accgaggctt
     1921 cctgtcgcgc atcgaacgcc gccgcccctt cgtccgcctc aaatgcgccg tttcgctgga
     1981 cggcaaaacc gccctttcag acggcagcag cttttggatt accggcgaag acgcgcgtgc
     2041 cgacgtacag gttttgcgtg ccgaaagctg cgcggtgctg accggcatcg gcacggtgtt
     2101 ggcggacaat ccccggctca acgtccgcgc ttttccaact ttgcgccaac ccgcacgcat
     2161 cgtgttagac agccgcctgc gcctgccctt ggacagccac cttctgaccg acggacaatc
     2221 tccaacctac atcgccacct tggaacgcga tgaagacaaa ctccgcccct atcgcaaaca
     2281 cgcccacgtc cgcatcctga tgccgtctga aacggcaggc ggcaaaatcg atttgaacag
     2341 cctgatgcgc ctgcttgccg acgaaggcat aggggaactc atagtcgaag caggctccga
     2401 actcacatct gcatttttgg cagaagattt ggcagacgaa atcgtgctgt accgttcgcc
     2461 caaaatcctc ggcagcggca aagacctgtt ttccctgccc gaaaaccgcg ccgccctttc
     2521 cgcaccgccc ttgtggatgc ccgtttccac cgaaatcctc ggacacgaca tcaaaaccgt
     2581 gttccggaaa aacgataacg ccttttaaag ggtttgcgcc gtttcactat a
//
LOCUS       DAWHSK010000247         2629 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.247, whole genome shotgun sequence.
ACCESSION   DAWHSK010000247 DAWHSK010000000
VERSION     DAWHSK010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.247"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(330..563)
                     /locus_tag="WIC03_001896"
     CDS             complement(330..563)
                     /locus_tag="WIC03_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEZ3884352.1"
                     /translation="MKLTLMFREYCSLCHKMRDELKPFQDEYGFGLEVVDVDENPVLE
                     EKYNELVPVLLAGDEEICHWFLDEDRLKQFLER"
     gene            complement(729..929)
                     /locus_tag="WIC03_001897"
     CDS             complement(729..929)
                     /locus_tag="WIC03_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HEZ3884353.1"
                     /translation="MFVCICNAVTDHQIKETIAAGATTMGDLQSQLGVASCCGCCGEL
                     AASFLTAHNAQPTVTAGINVQA"
     gene            complement(1101..1976)
                     /gene="xerD"
                     /locus_tag="WIC03_001898"
     CDS             complement(1101..1976)
                     /gene="xerD"
                     /locus_tag="WIC03_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEZ3884354.1"
                     /translation="MEEGLIDRLLETLWLDRRLSQNTLNGYRRDLEKIARRLSQSGRM
                     LKDADEADLAAAVYVDGEQRSSQARALSACKRLYIWMEREGIRTDNPTRLLKPPKIDK
                     NIPTLITEQQISRLLATPDTDTPHGLRDKALLELMYATGLRVSEAVGLNFGNVDLDRG
                     CITALGKGDKQRMVPMGQESAYWVERYYTEARPLLLKGRNCDALFVSQKKTGISRQLA
                     WMIVKEYASQAGIGHISPHSLRHAFATHLVQHGLDLRVVQDMLGHADLNTTQIYTHVA
                     NVWLQGVVKEHHSRN"
     gene            complement(2028..2468)
                     /locus_tag="WIC03_001899"
     CDS             complement(2028..2468)
                     /locus_tag="WIC03_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEZ3884355.1"
                     /translation="MDVKYEFTLPSSSGADFHSAEHLPLVVYFYPKDSTPGCTTEGLD
                     FNARLEQFEALGYTVVGISRDGVKAHQNFCAKQGFRFELLSDKDETVCRLFDVIKLKK
                     LYGKESLGIERSTFVLNKDGEIAHEWRKVKVAGHAQEVLETLSR"
BASE COUNT          704 a          748 c          523 g          654 t
ORIGIN      
        1 gttaatccac tataaaatct accgattaca caaacacata tcatctttac acaatcatgc
       61 ttccatcaac agtaaaacat gatatgattg ccaacaataa catctcacaa taaattttct
      121 aatttttatt gaaaaaatca ataaataaga attctcccaa ccgacaaata aaataaagaa
      181 agggtcaata tgcaacaccg tagaagattg acaatttacc aggcatccaa acgtgcttcc
      241 tttactggca ggtcatccgc cccgcaaaaa cgtaaagaac gttgatttga aaaaaatgcc
      301 gtctgaagtc ctgcttcaga cggcattttt taccgttcga gaaactgttt caacctgtcc
      361 tcatccaaaa accagtggca gatttcctca tctcccgcca acaaaacggg aaccagttca
      421 ttgtattttt cttccaaaac aggattttca tccacatcga ccacttccag cccgaacccg
      481 tattcatcct gaaaaggttt gagttcgtcg cgcattttgt ggcacaagct gcaatattca
      541 cgaaacatca aggtcaattt catccgcgtt tccttatctg tcaatttgca cacgccaaag
      601 ccttagacgc agcagaatca tggtctattt gggaaaaaac aatgttttca aggaagatga
      661 tactcaagtc ctgccaaaaa cagcaaaaat gccgtctgaa cagttcagac ggcatttcca
      721 aaaccgtttt acgcttgaac gttgataccc gccgtaaccg tcggttgcgc attgtgcgcc
      781 gtcagaaacg aagcggcaag ctccccgcag cagccgcagc agctcgctac gcccaattgc
      841 gactgcaaat cgcccattgt ggtcgcgccg gcggcgatgg tttccttgat ttgatggtcg
      901 gtaacggcat tgcagatgca gacaaacatt ttgtgctccg tgtgttctca aactatcgta
      961 ccgatagcgg cttttattat cgtatgcaaa tataaataaa aacggttcgc attgcaaggt
     1021 cggcatacac ggtttgtctt ggtaattttt tatcaagttt tcatttccga ttgatttctt
     1081 attaaaccga agcagaagcc tcagtttcgg gaatggtgtt ccttcactac accctgcaac
     1141 catacattgg caacatgggt gtaaatctgc gtcgtattca aatcggcatg tcccaacata
     1201 tcctgaacca cgcgcaaatc caatccgtgc tgcaccaagt gcgtggcaaa ggcgtggcgc
     1261 aggctgtgcg ggctgatgtg cccgatgcct gcctgacttg catattcttt gacaatcatc
     1321 catgccaact gacgagaaat gcccgtcttt ttctgactga caaacaatgc gtcgcaattc
     1381 ctgcctttca gcagaagtgg gcgtgcctcc gtataatagc gttccaccca atacgccgac
     1441 tcctgcccca tcgggaccat cctctgctta tcgcccttgc ccagcgcggt aatacagccc
     1501 ctgtccaaat ccacattgcc gaagttcagc ccgaccgcct cgctgacgcg caagccggtc
     1561 gcgtacatca attcgagcag tgccttgtcc cgcaaaccgt gtggcgtatc agtatccgga
     1621 gtggcaagca gccgggaaat ctgctgttct gtaatcagtg tcggaatatt cttgtcgatt
     1681 ttgggcggtt tcagcaaacg ggtgggattg tccgtcctta tgccttcacg ctccatccat
     1741 atatacaggc gtttgcacgc cgataatgcg cgcgcctgcg aactccgttg ctctccgtca
     1801 acataaaccg ccgccgccaa atccgcttcg tccgcatcct tcagcattct gcccgattgg
     1861 gacaggcggc gggcgatttt ttccaaatcg cgccggtaac cgtttaaagt attctgactg
     1921 agccgcctgt ccaaccacag cgtttcaagc agcctgtcga tcaaaccttc ttccataccg
     1981 ttccaaacaa atgccgtctg aatcttcttc agacggcatg gttcacatta tcgggaaagc
     2041 gtttccaaca cttcctgtgc atgacccgcc actttgactt tccgccattc atgggcgatt
     2101 tctccatcct tattcaagac gaacgtactg cgctcgatac ctaacgactc tttcccgtac
     2161 agtttcttca atttgatgac atcaaacagg cggcacactg tttcatcctt gtcgctcaac
     2221 agctcgaacc ggaaaccctg cttggcgcaa aaattctgat gcgcctttac gccgtcgcgg
     2281 gaaataccga ccacggtata acccaatgcc tcaaactgtt ccaaacgcgc attgaaatcc
     2341 aagccttccg tcgtacagcc aggcgtactg tctttcggat aaaaatacac gaccaaaggc
     2401 agatgttctg ccgaatgaaa atccgcaccg ctgctcgaag gcagggtaaa ttcatatttc
     2461 acatccatag tcctactccc gatattccca ttattcaaaa cggcacgcag acgcaccgcc
     2521 gcaattgcca aaccaacccc gattctaccg ccccaaagga caaggattca accgccggaa
     2581 acatccaaac cgacacacga cggcatgaaa aatatccatg tcaaaccac
//
LOCUS       DAWHSK010000248         2600 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.248, whole genome shotgun sequence.
ACCESSION   DAWHSK010000248 DAWHSK010000000
VERSION     DAWHSK010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.248"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..448)
                     /locus_tag="WIC03_001900"
     CDS             complement(<1..448)
                     /locus_tag="WIC03_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HEZ3884356.1"
                     /translation="MERQTDEFAQAAARAAIWFLARYAGSGSEVLANCTERLFQALQN
                     GHSFIRLSGGEADALSALAPVVGTSAAPLILEGRRLFLGRMWQLEYDLAAEIKRLAAA
                     GTSAPDAAGARQNLAKWFQGTGSEGQRDAAALALLQSFMVITGGPGT"
     gene            complement(516..1211)
                     /gene="lolD"
                     /locus_tag="WIC03_001901"
     CDS             complement(516..1211)
                     /gene="lolD"
                     /locus_tag="WIC03_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HEZ3884357.1"
                     /translation="MSELILKCEGVGKRYRDGGLDVRVLHGLDLEILAGESTGIIGSS
                     GSGKSTLLHILGGLDMPSEGRVLLMGEDLRTLSQRQLGILRNRHLGFVYQFHHLLPEF
                     SALENVMMPLLIGKKKREEAAETAMAMLEKVGLKHRSAHRAGELSGGERQRAAIARAL
                     VTQPKCLLADEPTGNLDRANARNVLDMMLELKTELGTGLVVVTHDDELAGRFERVMVM
                     KDGSLHPKQGANA"
     gene            complement(1204..2451)
                     /locus_tag="WIC03_001902"
     CDS             complement(1204..2451)
                     /locus_tag="WIC03_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HEZ3884358.1"
                     /translation="MFSLEAWIGLRYLRAKKRNGFMSFITMVSIAGIALGVTALIVVL
                     SVMNGFQKEIRGQLLNVAPHAEIGYIDNTDTDWRNLLRFTENRKGILAAAPYVSNQAL
                     LANAGEIRGVQMRGILPSEERKVVEYGDKMPAGKFEDLIPGEFDIILGVGLAEALGAE
                     VGNKVTVITPEGNVTPAGVVPRLKQFTVVGLVKTGVYEVDNSLAMTHIQDARVLYRLD
                     KEVAGLRLKLADPQNAPALTAKLIPEAQRDTVWVRDWTFSNRSYFEAVELEKRMMFII
                     LTLIIAVAAFNLVSSLVMAVTEKQADIAILRTLGLSPAGVMKIFMVQGAFSGFFGTLA
                     GVVCGVLLGWNVGRVVAFFENLLGVHLINSQVYFIDYLPSDVDMGDVALIACISLGLS
                     FVATLYPSWRASKTQPAEALRYE"
BASE COUNT          583 a          815 c          664 g          538 t
ORIGIN      
        1 ccgttcctgg cccgccggta atcaccataa aagactgcaa cagtgccaag gcggcggcat
       61 cgcgctgccc ttcgctgccc gtgccttgaa accattttgc gaggttttgc cgcgcgcctg
      121 ccgcgtcggg ggcggacgtg ccggctgccg ccaagcgttt tatctcggca gccaaatcgt
      181 attccaactg ccacatcctg cccaaaaaca gccttctgcc ttccaaaatc aaaggcgcgg
      241 cggatgttcc gaccacgggt gcgagtgccg acagcgcgtc agcctcgcca ccgctcaaac
      301 ggataaacga atgaccgttt tgcaatgcct gaaacaggcg ttcggtgcag tttgcaagca
      361 cttcgctgcc tgaacccgca tagcgtgcca aaaaccagat tgccgccctt gccgccgctt
      421 gggcaaattc atctgtctgc cgttccattg tattccttat ccaaatgccg tctgaaggcg
      481 tggggcttca gacggcgcgg tgttgtttcg gctgtttagg cgtttgcgcc ctgtttgggg
      541 tgcaggctgc cgtctttcat gaccatcacg cgctcgaagc ggccggcgag ttcgtcgtcg
      601 tgcgttacga ccaccagccc cgttcccaat tctgttttca gttccagcat catatccaaa
      661 acattcctgg cgttcgcacg atcgagattg ccggtcggtt cgtcggcaag caggcatttg
      721 ggttgcgtaa ccaaggcgcg tgcaatggcg gcacgctgcc gctcaccgcc ggacagttcg
      781 cccgcgcggt gcgccgaacg gtgcttcaat ccgacctttt ccagcatcgc cattgccgtt
      841 tctgccgcct cttcccgttt tttcttgccg atcagaagcg gcatcatcac gttttccagt
      901 gccgaaaatt caggcagaag atggtggaac tggtacacga aaccgagatg gcggttgcgc
      961 aggatgccca gttgtcgctg gcttaatgta cgcaaatcct cgcccatcag cagcaccctg
     1021 ccttcagacg gcatatccag cccgcccaaa atatgcagca gcgtcgattt gccgctgccc
     1081 gaagaaccga tgatgccggt actttccccc gcgaggattt ccaaatccaa gccgtgcagc
     1141 acccgaacgt ccaaaccgcc gtcacggtag cgtttgccca cgccttcgca tttcaaaatc
     1201 aactcactca taacgcaaag cctccgccgg ttgggttttt gacgcgcgcc agctcgggta
     1261 gagcgtcgcc acgaaagaca gtcccaaaga aatgcaggca atcagggcaa cgtcgcccat
     1321 atcgacatcg ctgggcaggt agtcgataaa ataaacctgc gaattgataa ggtggacacc
     1381 gagcaggttt tcaaaaaacg ccacgactct gccgacgttc caacccaaaa gcacgccgca
     1441 gaccacaccc gccagcgtgc cgaaaaagcc tgaaaacgcg ccctgcacca taaaaatctt
     1501 catcacgccg gcaggggaaa gacccaaagt ccgtaaaatc gcaatgtccg cctgcttttc
     1561 cgtaaccgcc atcaccaagg aagagacaag gttgaacgcc gccacggcga taatcagcgt
     1621 caggatgatg aacatcatcc gtttttccaa ctcgaccgcc tcaaaatagc ttcgattgct
     1681 gaacgtccaa tcgcgcaccc aaaccgtatc cctttgcgcc tccggaatca gctttgccgt
     1741 caaggcgggg gcgttttgcg ggtcggcgag tttcagccgc agccccgcaa cttccttatc
     1801 caagcggtac agtacgcgcg cgtcttggat atgcgtcatc gccaaggagt tgtccacttc
     1861 gtaaacaccc gtcttaacca gaccgaccac ggtaaactgt ttcaacctcg gtacgactcc
     1921 ggcgggcgta acattgccct ccggcgtgat gacggtaact ttattgccga cttccgcccc
     1981 caaagcctcc gccaagccga caccgaggat aatgtcaaac tcgcccggaa tcaggtcttc
     2041 aaatttgcct gccggcattt tgtcgccgta ttccaccact ttgcgctctt cagacggcaa
     2101 aatgccgcgc atctgcacgc ccctgatttc gcccgcattg gccagcaatg cctgattgga
     2161 aacatagggc gcggcagcca aaataccttt gcggttttcg gtaaatcgaa gcaggttgcg
     2221 ccaatccgta tccgtattgt cgatatagcc gatttcggcg tgcggcgcga cattcaggag
     2281 ctgcccgcgt atttctttct gaaagccgtt cataaccgac aagacgacaa tcagcgcggt
     2341 tacgcccaag gcgattccgg caatcgaaac catcgtgata aacgacataa agccgttgcg
     2401 ctttttcgcc ctgagatacc tcaagcctat ccaagcctct agagaaaaca taacgctacc
     2461 ttaaaaatgt ctgcaaacgt gccgccccgg acggcggttt gggggcggcg gcaaaagttt
     2521 attgtaccgt aaaacccagg cagcgtccga acgcccgagt gcgggggacg gggcggcaga
     2581 gcgggcggaa aacttgcaca
//
LOCUS       DAWHSK010000249         2576 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.249, whole genome shotgun sequence.
ACCESSION   DAWHSK010000249 DAWHSK010000000
VERSION     DAWHSK010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.249"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(139..936)
                     /locus_tag="WIC03_001903"
     CDS             complement(139..936)
                     /locus_tag="WIC03_001903"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HEZ3884359.1"
                     /translation="MNRLYPHPIIAREGWPIIGGGLALSLLVSMCCGWWSLPFWVFTV
                     FALQFFRDPAREIPQNPEAVLSPVDGRIVVVERARDPYRDVDALKISIFMNVFNVHSQ
                     KSPADCTVTKVAYNKGKFVNADLDKASTENERNAVLATTASGREITFVQVAGLVARRI
                     LCYTQAGAKLSRGERYGFILFGSRVDMYLPVDAQAQVAIGDKVNGVSTVLARLPLTAP
                     QIESEPESEPALQTAPVETAANPSAEQRQIEAVAAKIQAAVQDVLKD"
     gene            1091..>2576
                     /locus_tag="WIC03_001904"
     CDS             1091..>2576
                     /locus_tag="WIC03_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEZ3884360.1"
                     /translation="MCNHHPQHSHDNDTIRIRGARTHNLKNIDLDIPRHKLVVVTGLS
                     GSGKSSLAFDTLYAEGQRRYVESLSAYARQFLQMMDKPDVDLIEGLSPAISIEQKSTS
                     HNPRSTVGTVTEIHDYLRLLYARVGTPYCPEHNLPLSSQTVSQMVDAVLKLPEDTRVM
                     ILAPAVRERKGEFVDFFADLQAQGFARVRVDGEVYQLDEVPKLEKNIKHNIDVVIDRV
                     KVKANIKQRLAESFETALRHGNERALAMEMDSGEEHWFSARFACPVCSYSLPELEPRL
                     FSFNNPMGSCPTCDGLGNTNFFDSEKVVAHPELSLASGAIDGWDKRNQFYFQMIQSLA
                     RHYGFDVQAAWETLPAKIKKVVLHGSGKEVIDFTYLSERGTTFNRSHAFEGIIPNLER
                     RYRETDSETVREKLREYQNHRACPSCGGARLRKEARYVYVGGEPLHEVSAWPLTKTHR
                     FFETLDLDGNKKQIAEKILKEITERLGFLINVGLDYLNLSRSAET"
BASE COUNT          637 a          737 c          641 g          561 t
ORIGIN      
        1 taaatcgctg taacgttaag ttcccggatt cccattttcg tgaacataac ggtgtgaaca
       61 ttagcaaaaa ttcaaaaaac caaacttcct cattcgtgtg aatgatgata ctgatatttt
      121 ctatttcagt tcgcaaaatt aatctttcaa cacatcttgc acagccgcct gaatcttagc
      181 cgccactgcc tcgatttgcc gttgttcggc agatgggttt gccgctgttt caaccggagc
      241 agtttgtaaa gcaggctcag attcaggctc ggattcgatt tgcggcgcag tcagcggcaa
      301 acgcgccaat acagtgctga caccgtttac tttatcgcca atcgccactt gcgcctgcgc
      361 atcgacaggc agatacatat ccacgcgcga accgaagagg ataaagccat aacgttcgcc
      421 gcgggacagt ttcgcacctg cttgggtgta gcacaaaata cggcgcgcca ccaaaccggc
      481 cacttgaaca aaagtaattt cacgaccgga agccgtagtc gccaacaccg cattacgttc
      541 gttttccgtg ctggctttgt ccaaatccgc attcacgaat ttgcctttgt tataggccac
      601 tttcgttacc gtacaatcgg caggcgattt ttgcgaatgc acgttgaaca cgttcataaa
      661 aatactgatt ttcaaagcat cgacatcacg atacggatcg cgtgcgcgct cgaccaccac
      721 gatacggccg tcaacaggac tcaacaccgc ttcaggattt tgcggaatct cacgcgcagg
      781 gtcgcggaaa aactgcaaag caaatacggt aaacacccaa aacggcaaag accaccagcc
      841 gcaacacatc gacaccagca ggctcaaagc caaaccgccg ccaataatcg gccagccctc
      901 acgggcgata atcgggtggg ggtaaagacg gttcatttgt catccttttg ttcaagcggg
      961 ttaaaattgc cgccattata acggaatctg ccgcgttccg ccgacaaatg ccgtctgaac
     1021 gcggcttcag acggcatccg agaaagtgct acaatatcgg ttttccttta tttttcaggc
     1081 ggcatccatt atgtgcaacc atcatcccca acattcgcac gacaacgaca ccatccgcat
     1141 ccgcggcgcg cgcacgcata atttgaaaaa catcgattta gacattccgc gccacaagct
     1201 cgtggtggta acaggattgt cgggcagcgg caagtcgtcg ctggcatttg atactttata
     1261 tgccgaaggg cagcggcgtt atgtcgagag cctttctgcc tatgcgcggc agtttttgca
     1321 gatgatggac aagcccgatg tcgatttgat cgaaggcttg tcgcccgcga tctccatcga
     1381 gcagaaatcc accagccaca atccgcgttc caccgtcggc acggttacgg aaatccacga
     1441 ctacctgcgc cttttgtacg cccgtgtcgg tacgccgtat tgtcccgaac acaatctgcc
     1501 gctatccagc caaacagtat ctcagatggt tgatgccgtg ttgaagctgc cggaagacac
     1561 gcgcgtgatg attctggcgc cggcggtgcg cgagcgtaag ggcgagtttg tcgacttctt
     1621 tgccgacttg caggcgcagg gttttgcgcg cgtgcgcgtg gacggcgaag tttatcagtt
     1681 ggacgaagta ccgaagctgg aaaaaaacat caagcacaat atcgacgtgg tcatcgaccg
     1741 cgtgaaagtg aaggcgaaca tcaagcagcg gctggcggaa agttttgaaa ccgcgctgcg
     1801 ccacggcaac gagcgcgcgc tggcgatgga aatggacagc ggcgaagagc attggttttc
     1861 cgcgcgtttc gcctgccccg tatgttcgta cagcctgccc gaattggagc cgcgcctctt
     1921 ttcgttcaac aacccgatgg gctcctgccc gacttgcgac ggcttgggta acaccaattt
     1981 cttcgactcc gaaaaagtgg tcgcgcatcc cgaattgtcg ctggcttcgg gcgcgattga
     2041 cggctgggac aagcgcaacc agttctattt ccaaatgatt cagtcgctgg cgcggcatta
     2101 cggtttcgac gtgcaggctg cttgggaaac cctacctgca aaaatcaaaa aagtcgtgtt
     2161 gcacggctcg ggcaaagaag tcattgattt cacttacctg tccgaacgcg gcaccacctt
     2221 caaccgcagc cacgccttcg aaggcatcat ccccaatctc gaacgccgct accgcgaaac
     2281 cgacagcgaa accgtgcgcg aaaaactgcg cgaataccaa aaccaccgcg cttgcccgag
     2341 ctgcggcggc gcacgtttgc gcaaagaagc ccgctacgtt tacgtcggcg gcgagccgtt
     2401 gcacgaagtt tccgcctggc cgctgaccaa aacccaccga ttctttgaaa cgctggattt
     2461 ggacggcaac aaaaaacaaa tcgccgaaaa aatcctcaaa gaaatcaccg aacgtctcgg
     2521 ctttttgatt aatgtcgggc tggattacct gaatctctcc cgttccgccg aaaccc
//
LOCUS       DAWHSK010000250         2555 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.250, whole genome shotgun sequence.
ACCESSION   DAWHSK010000250 DAWHSK010000000
VERSION     DAWHSK010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.250"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(15..503)
                     /locus_tag="WIC03_001905"
     CDS             complement(15..503)
                     /locus_tag="WIC03_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884361.1"
                     /translation="MKKDIFYCEQWSYGYKKLHKPFSEKQAEEKHLKGELYTAVIGSA
                     TQPEYVITLREEVGFFSVHFFDKFGRDYLTHQFQKYSNSNYYFLSMAVWRDYITLESH
                     DLAEGYTYFFNENTDDCYVLKEDFINNERYEKTELYPQKDKVILFPKFGEYDLVLNPD
                     II"
     gene            complement(500..>2555)
                     /locus_tag="WIC03_001906"
     CDS             complement(500..>2555)
                     /locus_tag="WIC03_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HEZ3884362.1"
                     /translation="KRRRFLGISYSKAHDTTTQVMKTALPSRVVAESANLQSGWDTKL
                     QGTQFETTLGGATIRAGVGEQARADAKIILEGIKTTIHNETVSSSKSALWQKQAGRGS
                     NIETLQLPSFTGPVAPVLSAPGGYIADIPKGNLKTEIEKLAKQPEYAYLKQLQVAKNI
                     NWNQVQLAYDKWDYKQEGMTPAAAAVVVIVVTVLTYGALSAPAAAGTAGAAGAGAGGA
                     AAGTAAGTGVAAGTAATTGVAAGTSAAAITTAAGKAALASLASQAAVSLINNKGDINH
                     TLKELGKSSTVRQAATAAVTAGVLQGISGLNTQAAEAVSKHFHSPAAGKLTANLINST
                     AAASVHTAINGGSLKDNLGDAALGAIVSTVHGEVASKIKFNLSEDYIAHKIAHAVAGC
                     ASAVANKGKCRDGAIGAAVGEMVGETLLDGRDVGKLSPQERQKVIAYSQIIAGSVVAL
                     VKGDVNTAANAATVAVENNSLLARRRVNIRWTPRQELEHEYAILEIQAITNQIRRLDP
                     KFNGIAILRTPGEPWTRHDVQTYRQYYNQLRESRGFAVDPIYRIRINNGNEFNRIMSS
                     KYPYNELYVANPKSATGYFRVDSYNPATEEIISRKFTQFSQIQESTGIGYIKEAVRKY
                     SPGAVISNVPSTPTTIRGRKLEGKLILEVPAQVNPIPQSVLRAAQEENVIIRDTTGRI
                     YK"
BASE COUNT          553 a          614 c          585 g          803 t
ORIGIN      
        1 gccttaaaac tcaattaaat aatgtccgga tttaacacca aatcatattc gccaaacttt
       61 ggaaatagaa ttaccttatc tttttgggga tataattctg ttttttcata tcgctcatta
      121 ttaataaaat cctctttcaa aacatagcaa tcatccgtat tttcattgaa gaaataagta
      181 tatccttctg ccaaatcatg agattccaaa gttatataat ctctccatac agccatagaa
      241 agaaaataat aattcgaatt ggaatatttt tgaaattgat gggttaaata atcccttcca
      301 aatttatcga aaaaatgtac cgaaaaaaaa cctacttcct ctcgcaaggt aattacatat
      361 tcaggttgtg tcgccgaacc tattacggca gtatataact cccctttaag atgtttttcc
      421 tcagcttgtt tctcagaaaa aggcttatga agtttcttat aaccataaga ccactgctca
      481 caataaaaaa tatctttctt catttgtaaa tccttcctgt tgtatctcta atgataacat
      541 tttcttcttg tgccgccctt aatacagatt gtggaattgg attgacctga gcaggaactt
      601 ctaaaataag ttttccttca agctttcttc ctcttatcgt agtaggtgta cttggaacat
      661 tggaaatgac agcaccaggg ctatattttc taacagcctc cttgatataa ccaatccccg
      721 tactttcttg gatttgagaa aattgggtaa attttcttga aataatttcc tctgtcgcag
      781 gattatacga atctacccta aaataccccg tcgccgattt aggattggct acataaagct
      841 cattataagg gtattttgat gacatgatac ggttaaattc attgccgttg tttatcctga
      901 ttctataaat tgggtcaaca gcaaagcctc tggattccct taattgatta taatattgcc
      961 tgtatgtttg tacatcatgt cttgtccacg gctctccagg agtcctcaga atagcaatcc
     1021 cgttaaattt cggatccagc cttcggattt gattggtaat ggcctggatt tcaagaatgg
     1081 catattcatg ttccaattct tgtcgcggag tccaacgtat atttaccctc ctgcgagcta
     1141 aaagactatt attctccact gccacagtag ccgcattcgc cgccgtattc acatcccctt
     1201 taaccaatgc cactacgctg cctgcgataa tctgcgagta ggctatgact ttttggcgtt
     1261 cttggggtga cagtttgcct acatcgcgtc cgtccaacag ggtttctccc accatctcgc
     1321 cgactgccgc gccgattgcg ccgtcccgac atttgccttt atttgctacc gccgatgcac
     1381 agcctgctac ggcatgggct atcttgtggg caatgtagtc ttcgctgaga ttaaatttga
     1441 ttttgctcgc tacttctccg tgtacggtac tgactatcgc acccagtgcg gcatcgccca
     1501 agttgtcttt taggctgccg ccgttgatgg cggtatggac acttgcggca gcggtgctgt
     1561 tgatcaggtt agcggtcagt ttgcctgctg cgggactgtg aaaatgtttg ctgacggctt
     1621 cggctgcttg ggtgttcagc ccgcttatgc cctgcagtac gcctgcggtt acggcggcgg
     1681 tggcggcctg tctgacggtg ctgcttttgc ccagttcttt cagggtatgg tttatgtctc
     1741 ctttgttgtt gatgagggaa actgcggctt ggctggcgag actggccagt gcggctttgc
     1801 ctgcggctgt ggtgatagct gcagctgatg tgcctgctgc tactccggtt gtggctgccg
     1861 ttcctgctgc tactccagtt ccggctgccg ttcctgctgc ggctcctccc gctcctgcgc
     1921 ctgccgcgcc cgccgttccg gcggctgccg gggcggacag tgcgccgtag gtcaatacag
     1981 ttacgacgat aacgacgaca gctgctgctg cgggtgtcat gccctcctgt ttgtagtccc
     2041 atttatcgta agcaagctgc acctgattcc agttgatgtt tttcgcaact tgaagctgtt
     2101 ttaaataagc atactcgggc tgtttggcga gtttttcgat ttcggttttg aggttgcctt
     2161 tggggatgtc ggcgatatag ccgccgggtg cggacagtac gggcgcaacg ggaccggtga
     2221 aactcggcaa ttgcaaggtt tcgatgttac tgccccgtcc tgcctgtttt tgccatagag
     2281 cagatttgct gctgctcacg gtttcgttgt ggatcgtggt tttgatgcct tcgaggataa
     2341 tcttggcatc tgcccgtgcc tgctcaccta cgcctgcgcg tatggttgcg ccacccagtg
     2401 tggtttcaaa ctgtgtgcct tgcagtttgg tatcccaacc tgattgcaga ttggcagatt
     2461 ctgcaactac ccttgagggc agcgcggttt tcatgacttg ggtggtggtg tcgtgtgctt
     2521 tgctgtagct gatgccgaga aatctgcgcc ttttt
//
LOCUS       DAWHSK010000251         2533 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.251, whole genome shotgun sequence.
ACCESSION   DAWHSK010000251 DAWHSK010000000
VERSION     DAWHSK010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.251"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            300..2471
                     /locus_tag="WIC03_001907"
     CDS             300..2471
                     /locus_tag="WIC03_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ3884363.1"
                     /translation="MNTPLFRLSLLSLTLAAGFAHAAENNAKVVLDTVTVKGDRQGSK
                     IRTNIVTLQQKDESTATDMRELLKEEPSIDFGGGNGTSQFLTLRGMGQNSVDIKVDNA
                     YSDSQILYHQGRFIVDPALVKVVSVQKGAGSASAGIGATNGAIITKTVDAQDLLKGLD
                     KNWGVRLNSGFASNEGVSYGASVFGKEGNFDGLFSYNRNDEKDYEAGKGFRNVNGGKT
                     VPYSALDKRSYLAKIGTTFGGDDHRIVLSHMKDQHRGIRTVREEFAVGDTKSRINITR
                     QAPAYRETTQSNTNLAYTGKNLGFVEKLDANAYVLEKERYSADDSGTGYAGNVKGPNH
                     TRITTRGANFNFDSRLAEQTLLKYGINYRHQEIKPQAFLNGEFEISGKKKDPKDPKKE
                     IDKTAEEKAKDKKDMDLVHSYKLSNPTKTDTGAYIEAIHEIDGFTLTGGLRYDRFKVK
                     THDGKTVSSSNLNPSFGVIWQPHEHWSFSASHNYASRSPRLYDALQTHGKRGIISIAD
                     GTKAERARNTEIGFNYNDGTFAANGSYFWQTIKDALANPQNRHVSAAVREAVNAGYIK
                     NHGYELGASYRTGGLTAKVGVSHSKPRFYDTHKDKLLSANPEFGAQVGRTWTASLAYR
                     FQNPNLEIGWRGRYVQKAVGSILVAGQKDRNGKLENVVRKGFGVNDVFANWKPLGKDT
                     LNVNLSVNNVFNTFYYPHSQRWTNTLPGVGRDVRLGVNYKF"
BASE COUNT          718 a          732 c          581 g          502 t
ORIGIN      
        1 tccgatagat tcctgccgcg ttaggggttc tagattcccg ctttcgcggg aatgacgcgg
       61 aaaggttgct tttcgtcatt cccgcatttg ttaatccact atattcccgc cgttttttac
      121 atttccgaca aaacctgtca acaaaaaaca acacttcgca aataaaaacg ataatcagct
      181 ttgcaaaaat ccccccccct gttaatataa ataaaaataa ttattaatta tttttcttat
      241 cctgccaaat cttaacggtt tggatttact tcctttcata cactcaagag gacgattgaa
      301 tgaatacccc attgttccgt ctcagcctgc tctcgcttac cctggcggca ggttttgccc
      361 acgcggcaga aaataatgcc aaggtcgtac tggataccgt taccgtaaaa ggcgaccgcc
      421 aaggcagcaa aatccgtacc aacatcgtta cgctgcaaca aaaagacgaa agcaccgcaa
      481 ccgatatgcg cgaactctta aaagaagagc cgtccatcga tttcggcggc ggcaacggca
      541 cgtcccaatt cctgacgctg cgcggcatgg gtcagaactc tgtcgacatc aaggtggaca
      601 acgcctattc cgacagccaa atcctttacc accaaggcag atttattgtc gatcccgctt
      661 tggttaaagt cgtttccgta caaaaaggcg cgggttccgc ctctgccggt atcggcgcga
      721 ccaacggcgc gatcatcacc aaaaccgtcg atgcccaaga cctgctcaaa ggcttggata
      781 aaaactgggg cgtgcgcctc aacagcggct ttgccagcaa cgaaggcgta agctacggcg
      841 ccagcgtatt cggaaaagag ggcaacttcg acggcttgtt ctcttacaac cgcaacgatg
      901 aaaaagatta cgaagccggc aaaggtttcc gcaatgtcaa cggcggcaaa accgtaccgt
      961 acagcgcgct ggacaaacgc agctacctcg ccaaaatcgg aacaaccttc ggcggcgacg
     1021 accaccgcat cgtattgagc cacatgaaag accaacaccg gggcatccgc actgtgcgtg
     1081 aagagtttgc cgtcggcgac acaaaatcac ggataaatat tacacgccaa gcccctgctt
     1141 accgcgaaac tacccaatcc aacaccaact tggcgtacac gggtaaaaac ctgggctttg
     1201 tcgaaaaact ggatgccaac gcctatgtgt tggaaaaaga acgctattcc gccgatgaca
     1261 gcggcaccgg ctacgcaggc aatgtaaaag gccccaacca tacccgaatc accactcgtg
     1321 gtgcgaactt caacttcgac agccgccttg ccgaacaaac cctgttgaaa tacggtatca
     1381 actaccgcca tcaggaaatc aaaccgcaag cgtttttgaa cggcgaattt gagatctccg
     1441 gtaagaagaa agatccgaaa gatcccaaaa aagaaataga taagaccgct gaagaaaaag
     1501 cgaaagacaa gaaagatatg gatcttgtcc attcctacaa actgtccaac ccgaccaaaa
     1561 ccgataccgg cgcgtatatc gaagccattc acgagattga cggctttacc ctgaccggcg
     1621 ggctgcgtta cgaccgcttc aaagtgaaaa cccacgacgg caaaaccgtt tcaagcagca
     1681 accttaaccc gagtttcggt gtgatttggc agccgcacga acactggagc ttcagcgcga
     1741 gccacaacta cgccagccgc agcccgcgcc tgtatgacgc gctgcaaacc cacggtaaac
     1801 gcggcatcat ctcgattgcc gacggcacga aagccgaacg cgcgcgcaat accgaaatcg
     1861 gcttcaacta caacgacggc acgtttgccg ccaacggcag ctacttctgg caaaccatca
     1921 aagacgcgct tgccaatccg caaaaccgcc acgtctctgc cgccgtccgt gaagccgtca
     1981 atgccggtta catcaaaaac cacggttacg aattgggcgc gtcctaccgc accggcggcc
     2041 tgactgccaa agtcggcgta agccacagca aaccgcgctt ttacgatacg cacaaagaca
     2101 agctgttgag cgcgaatcct gaatttggcg cacaagtcgg ccgcacttgg acggcctccc
     2161 ttgcctaccg cttccaaaat ccgaatctgg aaatcggctg gcgcggccgt tatgttcaaa
     2221 aagccgtggg ttcgatattg gtggcaggtc aaaaagaccg caacggcaaa ttggaaaacg
     2281 ttgtacgcaa aggtttcggt gtgaacgatg tcttcgccaa ctggaaaccg ctgggcaaag
     2341 acacgctcaa tgttaatctt tcggttaaca acgtgttcaa cacgttctac tatccgcaca
     2401 gccaacgctg gaccaatacc ctgccgggcg tgggacgtga tgtacgcttg ggcgtgaact
     2461 acaagttcta aaacgcacat cccgaaaaaa tgccgtctga aaacctttca gacggcatct
     2521 gtcctgataa ttt
//
LOCUS       DAWHSK010000252         2527 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.252, whole genome shotgun sequence.
ACCESSION   DAWHSK010000252 DAWHSK010000000
VERSION     DAWHSK010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.252"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            281..1408
                     /locus_tag="WIC03_001908"
     CDS             281..1408
                     /locus_tag="WIC03_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ3884364.1"
                     /translation="MSNWKPDIPYNDLPPLPPKQDIESKTILKRCIAARASLARLKQA
                     AELIPNQAMLINTLPVMEARASSEIENIVTTTDKLFQSLQMDTERQDPATKEALQYRT
                     ALFAGYESLTSRPLCTQTAIMVCNAIKHPYEMAIRKTGGTALKGGNSGNVVYTPPEGE
                     ETIRGKLANWERFIHESGDLDPLIIMAAAHYQFEAIHPFTDGNGRTGRILNSLLLIEK
                     GLLDLPILYLSRYIIENRADYYRLLLGVTERQDWESWIIYILDGVADTADWTVSKIDA
                     IRRLFEQTRQHIRTHAQGIYTHELVNLLFEQPYTRIANLEAAGIAKRQTASKYLKELS
                     DIGVLQEIAIGRDKLFIHPRLMELLRGEGNSFTSFQSLVKA"
     gene            1438..2316
                     /locus_tag="WIC03_001909"
     CDS             1438..2316
                     /locus_tag="WIC03_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ3884365.1"
                     /translation="MARRSKTFEEAAAEVEERFGHRGIKLVEFEGTAKPCVINCPKHG
                     NQTCSRYSNMFIGSSWGCPSCGNEQAAKAGIATLRKNHIALEMLKQAVTGMTKQERIT
                     TQAYNEMTKSVAGSNSIVLNDVQGDTTINNHHTHTHNHSDADGKALSMRLTSRPLLSD
                     RQAAAFARTGKLTGSFDLFASVVAPSQYTFAVAMPDTSMSPVIEKGDLLVVEPRMCPA
                     DEDIALIELSDKRLVVAHLVIDIAGRMLIYQTGRPSEAFDLPEGSTILGVVLEVKKRF
                     MSAAQARRRVDSDYHP"
     gene            2320..>2527
                     /locus_tag="WIC03_001910"
     CDS             2320..>2527
                     /locus_tag="WIC03_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEZ3884366.1"
                     /translation="MDGWYDFRFQNVVYAPDRSPEISRMLSSILAGYAWDTENPFVAK
                     SEQRLSALSEWVGQLETEYSELNLN"
BASE COUNT          708 a          635 c          616 g          568 t
ORIGIN      
        1 tagtgtatat taagtacgtc tgatatacac gataccctac gagggtgtaa gctttagttc
       61 acatttaaaa tgacctcttt aaacctgtct ttcggcaggt ttctttttag gttgtttgga
      121 aatcgtgtgc agacaaggtg taaaataggt aacagcataa aataatgcgg ttttaccgcc
      181 catatattta caaaagccaa atttttaaac atatatcctt gatatataca cggcgtaaac
      241 atatactgga aacatcttta aattttttga aattttaaat atgagcaact ggaaacccga
      301 tattccctat aacgatttac cacccctgcc gccaaaacag gatattgaaa gcaaaaccat
      361 cctgaaacgt tgtatagccg cccgtgcatc ccttgcccgt ttaaagcagg cggcagaact
      421 gataccgaat caagccatgc tgattaacac ccttcctgtt atggaagccc gtgcaagttc
      481 agaaattgaa aacatcgtaa ccacaacgga taagctgttt caatccctgc aaatggatac
      541 ggaacggcaa gaccctgcca cgaaagaagc cctgcaatac cgcaccgccc tgtttgcagg
      601 ctatgaatca ctgacgagcc gccctttatg cacacaaacc gccatcatgg tctgcaacgc
      661 catcaagcac ccctacgaaa tggccatccg caaaacaggc ggcacagccc taaaaggagg
      721 caacagcgga aatgttgtct ataccccgcc cgaaggagaa gaaaccatac gcggcaagct
      781 ggcaaattgg gagcggttta ttcacgaaag cggcgattta gacccgctta tcatcatggc
      841 ggcggcacat taccaatttg aagccatcca tccgtttacg gacggcaacg ggcggacggg
      901 gcgcatattg aacagcctgc tattgattga aaaagggctt ttggatttgc ctattttgta
      961 tttgagccgc tacatcatcg aaaacagggc ggactattac cgcctgcttt taggcgtaac
     1021 cgaacggcag gattgggaaa gctggataat ctacatctta gacggcgtag ccgacaccgc
     1081 cgattggacg gtatcgaaaa tagatgcgat acgccgcctg ttcgagcaga cacgtcaaca
     1141 catacggaca cacgcacaag gaatctacac gcacgaactg gtaaatcttc tgtttgagca
     1201 gccatataca cgcattgcca acctagaagc ggcagggata gccaaacggc agacggcctc
     1261 taagtacctg aaagagcttt cagacatagg tgtgctgcaa gaaatcgcca ttggcaggga
     1321 caaactattc attcatccgc gcctaatgga actattgcgg ggagagggca acagctttac
     1381 ctcattccaa tccctcgtta aagcatagcc aaaataatca ataatccgga ggtcaatatg
     1441 gcaagaaggt caaaaacatt tgaagaagct gctgctgagg ttgaggaacg tttcggtcat
     1501 cgtggcatta agttggtcga gtttgagggt acagccaagc cgtgtgtaat caactgccct
     1561 aaacatggaa accaaacctg ttcgaggtac tccaatatgt tcataggaag tagctggggt
     1621 tgcccctctt gtggtaatga gcaagctgca aaagccggta tagcgaccct taggaagaat
     1681 cacatagcgt tagaaatgct gaaacaggct gtaacaggta tgaccaagca agagcgcatc
     1741 acgacgcaag cctacaatga gatgaccaaa tccgtggcag gttcaaacag catagtcctt
     1801 aacgatgtcc aaggcgatac gaccatcaac aaccatcata cgcatacgca caaccacagc
     1861 gatgccgatg gcaaagcact gtcgatgagg ctcacatccc gtcctttgtt gtcagaccgt
     1921 caggcggcgg ctttcgcccg tacaggcaaa ctcacgggca gtttcgacct gtttgcttcg
     1981 gtggtcgccc cctcgcagta cacgtttgcc gttgccatgc ccgacacgtc catgtcgccg
     2041 gttatcgaaa agggagactt gctggtggtc gagccgcgta tgtgccctgc ggacgaagac
     2101 atcgcgctga ttgaactgtc cgacaagcgg ctggtcgtcg cgcaccttgt tatcgatatt
     2161 gcgggcagga tgctgattta tcagacgggc aggccgtctg aagcctttga cctgcccgaa
     2221 ggcagcacga ttttaggtgt ggtgctggag gtcaaaaaac ggtttatgtc cgccgcacag
     2281 gcgagaaggc gtgttgattc ggattaccac ccctgatgtg tggacggctg gtacgatttc
     2341 cgcttccaaa acgtcgtgta cgcgcccgac cgcagcccgg aaatcagccg tatgctttct
     2401 tcgattttgg caggctacgc gtgggatacc gaaaacccgt tcgtggcgaa atccgaacaa
     2461 cgcctgtcgg ccttgtccga atgggtcggt cagctggaaa ccgaatatag tgaattaaat
     2521 ttaaacc
//
LOCUS       DAWHSK010000253         2498 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.253, whole genome shotgun sequence.
ACCESSION   DAWHSK010000253 DAWHSK010000000
VERSION     DAWHSK010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.253"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(104..925)
                     /gene="nadE"
                     /locus_tag="WIC03_001911"
     CDS             complement(104..925)
                     /gene="nadE"
                     /locus_tag="WIC03_001911"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HEZ3884367.1"
                     /translation="MMRIFSTGGTMDTQAVITHIARWLDEYATQANAKGFVIGVSGGI
                     DSAVVSTLAARTGRPTLLLDMPIRQHPGQLERARLHIRNLQRQYANVSAQTADLTDTF
                     QTFEQTVGIHQTAFDNQPLSLANAKSRLRMLTLYYYGQIHGLLVTGTGNKIEDFGVGF
                     FTKYGDGGVDISPIADLTKTQVYRLAEALGVDEAIQKAPPTDGLWDTERTDEEQMGAS
                     YPELEWAMGVYGTRKPEDFEGRQREVLEIYTRLHRAMQHKINPIPVCRIPPELLG"
     gene            1122..2477
                     /locus_tag="WIC03_001912"
     CDS             1122..2477
                     /locus_tag="WIC03_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEZ3884368.1"
                     /translation="MSDFFHSDVLSVSELNAFAKSILENHLAGLWIAGEVSNLTRAAS
                     GHYYFSLKDSRAQVRCAMFKGAAARLAKPLKEGDHIEVAGKISIYETRGEFQITVNEV
                     RLKGLGQLYEAYERLKAQLQAEGAFAEERKKPLPVRPQCIGIVTSLAAAALRDVVTTL
                     KRRAPEIPVIVYPTPVQGTGSEFQIAQAIKTASQRAECDVLIVCRGGGSIEDLWAFNE
                     EPVVRAIEACTVPVVSGVGHETDFTLADFVADVRAPTPTGAAELVSPNRQESLHRLAQ
                     AQGRLKTVLEQRYFDASQKLDWLARQIRHPRQKLDEQRASIGKLAQTLSYSMKQNLRA
                     HTARFERQTQALQHYRPDVSVCKNNIVRLQTALPAAFSQLLTHRRQSLTAQAALLEAV
                     SPQHILERGFSVVKDTRGQVIRNADVLKQGQKLHITFADGETDVRVSKEQGQQDLFDC
                     I"
BASE COUNT          537 a          691 c          685 g          585 t
ORIGIN      
        1 gatattctgt ttgcccgcgt actcgattct taaggaacgg gagccggata tttccatgcc
       61 gtctgaaata cggtttttcc gcttcagacg gcatttccac gtttcagccc aacaattcgg
      121 gcggaatgcg gcacacggga atcgggttga ttttgtgctg catcgcgcgg tgcagccgcg
      181 tatagatttc cagaacttcg cgctgccgcc cttcaaaatc ttcgggtttg cgcgtgccgt
      241 acacgcccat tgcccactcc agttcgggat agcttgcgcc catctgttct tcgtcggtgc
      301 gttccgtatc ccacaggccg tcggtcggcg gggctttttg aatcgcctcg tccacgccca
      361 atgcttcggc aagccggtaa acctgcgttt tggtcaggtc ggcaatcggg ctgatgtcta
      421 cgccgccgtc gccgtatttg gtaaaaaatc ccacgccgaa atcttcaatc ttattacctg
      481 tccccgtaac cagcagtccg tgtatctgcc cgtagtagta cagggtcagc atacgcaggc
      541 ggcttttggc gttggcgagg gaaagcggct gattgtcaaa tgccgtctga tgaatgccga
      601 cggtttgttc aaaggtctgg aaggtgtcgg tcaaatcggc cgtttgcgcg cttacattgg
      661 catattgccg ttgcagattg cggatgtgca gccttgcccg ctcaagctgg ccggggtgtt
      721 ggcgtatcgg catatccaga agcagcgtgg ggcggccggt gcgggcggcg agtgtggaga
      781 cgacggcgga atcgatgccg ccggaaacgc ctatgacgaa tccttttgca tttgcctgag
      841 tggcgtattc gtccaaccat cgggcgatat gtgtgataac tgcctgcgta tccatggttc
      901 ctcctgtcga aaatatccgc atcatagctt gtcggcggtt caatttgaat agattgcagg
      961 caggttgatg aatatttttc ccattccccg cttaaaaaag tcatacgggc aaagtcgggc
     1021 ggcaagatgg cgcgtgttat aatattgttg acaatttagg cggctctatg ccgccttggg
     1081 cttcaaaaca tattcatatt ttttactatt tgattgattc gatgtctgat tttttccatt
     1141 ctgacgtgtt gtccgtttcc gagttgaacg catttgccaa aagtattttg gaaaaccacc
     1201 ttgccggtct gtggattgcg ggcgaagtgt ccaacctgac ccgtgccgcc agcgggcatt
     1261 attatttctc gctcaaagac agccgcgcgc aggtgcgttg cgcgatgttc aagggtgcgg
     1321 cggcgcgttt ggcgaaacct ttgaaagaag gcgaccatat cgaagtggcg ggcaaaatca
     1381 gtatttatga aacgcggggc gaatttcaga ttaccgtgaa cgaagtgcgg ctcaaaggtt
     1441 tggggcagct ttacgaagcc tatgagcggt tgaaggcgca gttgcaggcg gaaggcgcgt
     1501 ttgcggagga acgcaaaaaa ccgttgcccg tccgccctca atgtatcggc atcgtaacca
     1561 gtttggcggc ggcggcattg cgcgatgttg taaccacctt gaaacgccgc gcgcccgaaa
     1621 ttcccgttat cgtttatccg acacctgttc aaggcacagg cagcgagttt cagattgccc
     1681 aagcgattaa aaccgcatcg caacgcgccg aatgcgacgt gttgattgtc tgtcgcggcg
     1741 gcggcagcat tgaagatttg tgggcgttta acgaagaacc ggtcgtacgc gcaatcgaag
     1801 cctgcacggt tcccgtcgtc agtggcgtgg gacacgaaac cgatttcacg cttgccgatt
     1861 ttgtcgccga cgtgcgcgcg cccacgccga ccggcgcggc ggaactggtc agccccaacc
     1921 gccaagaatc gctacatcgt ctcgcccagg cgcaaggccg tctgaaaacc gttttggaac
     1981 aacgctattt cgatgccagc caaaaactcg actggcttgc gcggcaaatc cgtcatccgc
     2041 gccaaaaact tgatgagcag cgcgcttcaa tcggcaagct ggcgcaaacg ctgtcttact
     2101 ctatgaagca aaacctccgt gcccacaccg cccgttttga acgccaaacc caagccctgc
     2161 aacattaccg tcctgatgtt tccgtttgca aaaacaacat cgtccgcctt cagacggcat
     2221 tgcccgccgc cttcagccaa ctgcttaccc accgccgcca aagcctgacc gcccaagccg
     2281 cattgctcga agccgtttcg ccgcagcaca ttttggaacg cggcttttcc gtcgtcaaag
     2341 atacacgcgg acaagtcatc cgcaacgccg atgttttgaa gcaggggcag aagctgcaca
     2401 ttaccttcgc cgacggcgaa accgacgtgc gcgtaagcaa agaacaaggg cagcaggatt
     2461 tgttcgattg catttaaacc caatcggaaa aaccgctc
//
LOCUS       DAWHSK010000254         2486 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.254, whole genome shotgun sequence.
ACCESSION   DAWHSK010000254 DAWHSK010000000
VERSION     DAWHSK010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.254"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(227..1861)
                     /gene="groL"
                     /locus_tag="WIC03_001913"
     CDS             complement(227..1861)
                     /gene="groL"
                     /locus_tag="WIC03_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271863.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEZ3884369.1"
                     /translation="MAAKDVQFGNEVRQKMVNGVNILANAVRVTLGPKGRNVVVDRAF
                     GGPHITKDGVTVAKEIELKDKFENMGAQMVKEVASKTNDVAGDGTTTATVLAQSIVAE
                     GMKYVTAGMNPTDLKRGIDKAVAALVEELKNIAKPCDTSKEIAQVGSISANSDEQVGA
                     IIAEAMEKVGKEGVITVEDGKSLENELDVVEGMQFDRGYLSPYFINDAEKQIAGLDNP
                     FVLLFDKKISNIRDLLPVLEQVAKASRPLLIIAEDVEGEALATLVVNNIRGILKTVAV
                     KAPGFGDRRKAMLQDIAILTGGTVISEEVGLSLEKATLDDLGQAKRIEIGKENTTIID
                     GFGDAAQIEARVAEIRQQIETATSDYDKEKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KDRVEDALHATRAAVEEGVVAGGGVALLRARAALENLHTGNADQDAGVQIVLRAVESP
                     LRQIVANAGGEPSVVVNKVLEGKGNYGYNAGSGEYGDMIEMGVLDPAKVTRSALQHAA
                     SIAGLMLTTDCMIAEIPEEKPAMPDMGGMGGMGGMM"
     gene            complement(1955..2242)
                     /locus_tag="WIC03_001914"
     CDS             complement(1955..2242)
                     /locus_tag="WIC03_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEZ3884370.1"
                     /translation="MTIRPLHDRVVVKRLEAEEKTASGIVLPGAAAEKPDMGEVIAVG
                     AGKIGKDGARRPLDVKVGDKIIFGKYSGQTVKADGEELLVMREEDIFGIVE"
BASE COUNT          504 a          653 c          652 g          677 t
ORIGIN      
        1 ttcgcgggaa tgacggcgga aaggttgctg tttttccgat aaattcctgc cgctcttcgt
       61 ttttgggatg gcgggaaata aaacaaaagc gcgcgtatca aaaaataaca atgcaaagaa
      121 cggtgttggc aattttaagg tttgcgcctt agccgcactt attcgcaaaa aaaccgcacg
      181 gcgttgaccg tgcggcttgt tgtctgaagg cttcagacgg cattgcttac atcatgccgc
      241 ccataccgcc cataccgccc atgtcaggca tagcaggttt ttcttcaggg atttcggcaa
      301 tcatacaatc agtggtcagc atcaagccgg cgatggacgc ggcgtgttgc agcgcggaac
      361 gggttacttt ggcggggtcg agtacgccca tttcgatcat gtcgccgtat tcgccggagc
      421 ctgcgttgta accgtagttg cctttgcctt ccaacacttt gttcaccacc acgctaggtt
      481 cgccgcctgc gttggcaacg atttggcgca gcggagactc aacggcgcgc aagacgattt
      541 gtacgcctgc gtcttggtcg gcattgccgg tgtgcaggtt ttccagagcg gcacgggcgc
      601 gcaacagggc tacgccgccg cctgcgacca cgccttcttc aacggcggca cgggtcgcgt
      661 gcagcgcgtc ttctacgcgg tcttttttct ctttcatttc gacttcggta gctgcaccaa
      721 ctttgattac tgccacgccg cctgccagtt tggcaacgcg ctcttgcagt ttttctttgt
      781 cgtaatcgct ggttgcggtt tcgatttgtt ggcggatttc ggcaacacgc gcttcaattt
      841 gggcagcgtc accgaagccg tcgatgatgg tggtgttttc tttaccgatt tcgatgcgtt
      901 tggcttgacc caagtcgtcc aaagtcgctt tttctagaga caggccgact tcttcggaaa
      961 tcaccgtgcc gccggtcagg atggcgatgt cttgcaacat ggctttgcgg cggtcgccga
     1021 agcccggagc tttcacggca acggttttca ggatgccgcg gatgttgttc accaccaaag
     1081 tcgccaaggc ttcgccttct acgtcttcag cgataatcaa gagcgggcgg ctggctttgg
     1141 ccacttgttc caaaacaggc aacaaatcgc ggatattgct gatttttttg tcgaacagca
     1201 atacaaacgg attgtccaag ccggcgattt gtttttccgc atcgttgatg aagtaaggag
     1261 acaggtagcc gcggtcgaat tgcatacctt caaccacgtc cagctcgttt tccaaagatt
     1321 tgccgtcttc aacggtaatc acgccttctt tgccgacttt ttccatcgct tcggcaataa
     1381 tcgcgccgac ttgttcgtca gagttggcgg aaatagagcc gacttgggcg atttctttag
     1441 aagtgtcgca aggtttggcg atgtttttca gctcttcaac caaagcggcg acggctttgt
     1501 cgataccgcg tttcaggtcg gtcgggttca taccggcggt aacgtatttc ataccttcgg
     1561 caacgatgga ttgcgccagt acggtggcgg tagtcgtacc gtcgcccgcc acgtcgttgg
     1621 ttttggacgc gacttctttt accatttgcg cgcccatatt ttcaaacttg tctttcagtt
     1681 cgatttcttt agcgacggtt acgccgtctt tggtaatgtg cgggccgccg aaagcgcggt
     1741 caaccaccac gttgcggcct ttgggaccca gggttacgcg cacggcgttt gccagaatgt
     1801 tcacgccgtt taccattttt tgacggactt cattgccgaa ttgtacgtct tttgctgcca
     1861 tttcaattct ccaaaaatca ttaaaactgt ctgataaaac cgtttatgcc gtctgaaggc
     1921 ggtttgccgt ttcagacggc atcatcattc gcacttattc aacgatgccg aaaatatctt
     1981 cttcgcgcat taccaacagc tcttcgccgt cggcttttac ggtttggccg ctgtatttgc
     2041 cgaaaatgat tttgtcgccg actttgacat ccagcggacg gcgcgcgccg tctttaccga
     2101 ttttgcccgc gcccacggcg atgacttcgc ccatatcggg tttttcggcg gccgcacccg
     2161 gcaaaacgat acccgatgcg gttttttctt cagcttccaa gcgtttgacg acgacgcggt
     2221 cgtgtaaagg acggatggtc atatttatgc tccgataaaa tggtttgaaa acaatcatct
     2281 gcccgaacgg ttcggacaga ttgaagtgga aaccgggctg ccgcctgccg gccgcccgta
     2341 tgaatcggca aaattagggt gtgtgcgggc aaattcaagt gaggcggaaa aaatttattt
     2401 ccgccgtttt ttatagtgga ctaaatttaa ggggctttac tagattagca gatatgttac
     2461 cctcgaaata tgaagataac gcactg
//
LOCUS       DAWHSK010000255         2475 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.255, whole genome shotgun sequence.
ACCESSION   DAWHSK010000255 DAWHSK010000000
VERSION     DAWHSK010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.255"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            22..1632
                     /gene="pgi"
                     /locus_tag="WIC03_001915"
     CDS             22..1632
                     /gene="pgi"
                     /locus_tag="WIC03_001915"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEZ3884371.1"
                     /translation="MWNHFDDSKTLHMREMFEQDPQRAERYWLQVGGLTLDYSKNRIN
                     DETMSLLFELAREAGVPERMRQMFHGEKINTTENRAVLHVALRNRTNSPIVVDGEDVM
                     PKVNRVLQRMGEFAHEVRSGSWLGYTNQVITDVVNIGIGGSDLGPLMMCTALKPFGHP
                     RLNMHFVSNVDGSQLRDVLSKVHPETTLFIIASKTFTTQETLTNALTAREWFLNHAGD
                     EEAVAKHFAAVSTNQKAVAEFGIDTANMFEFWDWVGGRYSLWSAIGLPIMLYLGEENF
                     IEMLNGAHLMDQHFINTPLERNLPVILALIGIWYINYYGGGSHVIAPYDQHLHRLPKF
                     IQQLDMESNGKQVTLDGKAVGHETSPIIWGETGINGQHAFFQLLHQGTHITPIDLIAS
                     LEKRSNLPGHHEILLANVFAQAEAFMRGKTPDEVRAELKAQGMDEARIEELVPHKTFS
                     GNRPTNLILMDKVNPRNMGSLIAMYEHKTFVQGIIWGINSFDQWGVELGKQLAKTILG
                     ELTGETGPQKHDSSTERLINLYLQTNRK"
     gene            1646..2197
                     /locus_tag="WIC03_001916"
     CDS             1646..2197
                     /locus_tag="WIC03_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HEZ3884372.1"
                     /translation="MPSERRPFQTAFLSNRKTVGKLAERVQSRYDGLHKFKHICSAAM
                     ALIKEPLDKAKQRNEELEAAEEAAAQEALGREQEVDRVSEWEERYKLSRSEFEQFWKG
                     LPQTVQNKLQASQKTWKSGMDKICANNAKAEGETPNGIKFSELACKTAETEARLEELH
                     NRKKALIDEMAREADKKELPKRL"
BASE COUNT          689 a          700 c          626 g          460 t
ORIGIN      
        1 gacttacccg catggtcgaa attgtggaac cactttgacg acagcaaaac attgcatatg
       61 cgcgaaatgt tcgagcaaga cccgcagcgt gcggaacgct actggctgca agtcggcgga
      121 ctgacgctgg actactccaa aaaccgcatc aacgacgaaa ccatgtcgct tttgttcgag
      181 cttgcccgag aagcaggcgt gccggagcgg atgcggcaga tgttccacgg cgaaaaaatc
      241 aataccaccg aaaaccgcgc cgtcctgcat gtcgcccttc gcaaccgcac caattcgccg
      301 attgtggttg acggtgaaga tgtgatgccc aaagtcaacc gcgttttgca acgtatgggc
      361 gaatttgcac acgaagtccg cagcggaagc tggctgggct ataccaacca agtcattacc
      421 gacgttgtca acatcggcat cggcggatcg gatttgggcc cgctgatgat gtgtaccgcg
      481 ctcaaacctt tcggtcatcc gcgcctcaat atgcacttcg tctccaacgt ggacggctcg
      541 caactgcgcg acgtattgtc caaagtccac cccgaaacca cgctgttcat catcgcctcc
      601 aaaacattta ccacgcagga aacgctgacc aacgcgctga ccgcgcgcga atggtttttg
      661 aatcatgcgg gcgacgaaga agccgttgcc aaacacttcg ccgccgtttc caccaatcaa
      721 aaagccgtcg ccgaattcgg catcgacacc gccaatatgt ttgaattttg ggattgggtc
      781 ggcggtcggt acagcctgtg gtccgccatc ggattgccga ttatgctgta tctcggcgaa
      841 gaaaacttca ttgaaatgct caacggcgcg cacctgatgg accaacactt catcaacaca
      901 ccgctcgagc gcaacctgcc cgtcattctc gccctcatcg gcatctggta tatcaactac
      961 tacggcggcg gcagccacgt catcgcgcct tacgaccaac atttgcaccg cctgcccaaa
     1021 ttcatccagc agctcgatat ggaaagcaac ggcaaacagg ttacgttgga cggcaaagca
     1081 gtcggacacg aaacctcgcc gattatctgg ggcgaaacgg gcattaacgg ccagcacgcc
     1141 ttcttccaac tgctgcacca aggcacgcac attaccccca tcgacctgat tgcctcgctt
     1201 gaaaaacgca gcaacctgcc cggacaccac gaaatcctgc ttgccaacgt cttcgcccaa
     1261 gcagaagcct ttatgcgcgg caaaaccccc gacgaagtcc gtgccgaact caaagcgcag
     1321 ggtatggatg aggcgcgcat cgaagagctg gtcccgcaca aaaccttctc cggcaaccgc
     1381 ccgaccaacc tcattctcat ggacaaggtc aacccgcgca atatgggcag cctgattgcc
     1441 atgtacgaac acaaaacctt cgtacaaggc atcatttggg gcatcaacag cttcgaccag
     1501 tggggcgtgg aactcggcaa acaactggct aaaaccattt tgggcgaact gaccggcgaa
     1561 accgggccgc aaaagcacga cagttcgacc gaacgcctga tcaacctcta cctgcagacc
     1621 aaccgcaaat aaaacctgcg gaaaaatgcc gtctgaacgc cgaccgtttc aaacggcatt
     1681 tttatcgaac aggaaaaccg tcggtaaact tgcagagcgt gtgcaatccc gatatgatgg
     1741 tttgcataaa tttaaacata tatgttccgc agctatggca ctgattaaag agccgttgga
     1801 caaagcgaaa caaaggaacg aagaacttga agcggcagaa gaagcggcgg cgcaggaggc
     1861 attgggtcgg gagcaggaag tcgaccgcgt atccgaatgg gaagaacgct acaagctgtc
     1921 gcgcagcgag ttcgagcaat tctggaaagg attgcctcaa accgtacaga ataagctgca
     1981 agcctcacag aaaacatgga aaagcgggat ggataaaatc tgtgccaaca atgcgaaagc
     2041 tgaaggtgaa acgccaaacg gcataaaatt cagcgaactg gcatgcaaaa cggcggaaac
     2101 cgaagcacgc ttggaagagc tgcacaaccg taaaaaagcc cttatcgacg aaatggccag
     2161 ggaagcggac aagaaagaac tgccaaagcg gctctgaaca gcgcggttca ggcactgccc
     2221 gccgatattg ccgaaaccgt catgcccgag taccgcaact gacaaaacgg tttgaatgcc
     2281 aaatgcgccg acggcgacga atacggcgtg gcacaatctg actgccgtac cagagaaatc
     2341 aatgcgaaaa ccaaagaaat ccaaggttat ctgattgact gaaacttgga tgcgggaatg
     2401 tcggcggctt ttgcgttttt gtcgttttta tagtgaatta aatttaaacc gatacagcgt
     2461 tggctcgcct tagct
//
LOCUS       DAWHSK010000256         2459 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.256, whole genome shotgun sequence.
ACCESSION   DAWHSK010000256 DAWHSK010000000
VERSION     DAWHSK010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.256"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            52..1407
                     /locus_tag="WIC03_001917"
     CDS             52..1407
                     /locus_tag="WIC03_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC45 family MFS transporter"
                     /protein_id="HEZ3884373.1"
                     /translation="MSEYTPQTAKQGLPALAKSTIWMLSFGYLGVQTAFTLQSSQMSR
                     IFQTLGADPHNLGWFFILPPLAGMLVQPIVGYYSDRTWMPRLGGRRLPYLFYGTLIAV
                     IVMILMPNSGSFGFGYASLAALSFGALMIALLDVSSNMAMQPFKMMVGDMVNEEQKSY
                     AYGIQSFLANTGAVVAAILPFVFAYIGLANTAEKGVVPQTVVVAFYVGAALLVITSAF
                     TIFKVKEYDPETYARYHGIDVAANQEKANWFELLKTAPKAFWTVTLVQFFCWFAFQYM
                     WTYSAGAIAENVWHTTDASSVGYQEAGNWYGVLAAVQSVAAVICSFVLAKVPNKYYKA
                     GYFGCLALGALGFFSVFFIGNQYALVLSYTLIGIAWAGIITYPLTIVTNALSGKHMGT
                     YLGLFNGSICMPQIVASLLSFVLFPMLGGLQATMFLVGGVVLLLGAFSVFLIKETHGG
                     V"
     gene            1411..2430
                     /locus_tag="WIC03_001918"
     CDS             1411..2430
                     /locus_tag="WIC03_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEZ3884374.1"
                     /translation="MSDTPATPATRDFGLIDGRAVTGYVLSNRRGTRVCVLDLGGIVQ
                     EFSVLADGVRENLVVSFDDAASYADNPFQINKQIGRVAGRIRGAAFDINGRTYRVEAN
                     EGRNALHGGSHGLAVTRFNAVAADGRSVVLRSRLQQSADGYPNDLDLDISYRLDEDDR
                     LTVTYRATALGDTVFDPTLHIYWRLDAGLHDAVLHIPQGGHIPADAEKLPVSTVSDGL
                     EVFDFSRPKPLDAAVAALRRETGRAGFDDAYRVPSDIGRPAAVLQAGRRCRISIYSDR
                     NGLVIFTAAPQDFARHDAGVYDALATEAQTLPDSLHWPEFGNIRLNKGDTREATIAYG
                     IESLS"
BASE COUNT          449 a          669 c          735 g          606 t
ORIGIN      
        1 tggtttaaat ttaatccact atacttttca aatcaaaaaa ggatttacct tatgtcggaa
       61 tatacgcctc aaacagcaaa acaaggtttg cccgcgctgg caaaaagcac gatttggatg
      121 ttgagcttcg gctatctcgg cgttcagacg gcctttaccc tgcaaagctc acaaatgagc
      181 cgcatttttc aaactttagg cgcagacccg cataatttgg gctggttctt tatcctgccg
      241 ccgctggcgg ggatgctggt gcagccgatt gtcggttatt actccgaccg cacttggatg
      301 ccgcgattgg gcggccgccg cctgccgtat ctgttttacg gcacgctgat tgcggtcatc
      361 gtgatgattt tgatgccgaa ctcgggcagc ttcggtttcg gctacgcttc tttggcggct
      421 ttgtcgttcg gcgcgctgat gattgcgctg ctggacgtgt cttccaatat ggcgatgcag
      481 ccgtttaaga tgatggtcgg cgatatggtc aacgaggagc agaaaagcta cgcctacggg
      541 attcagagct ttttggcgaa taccggagcg gtcgtggcgg cgatcctgcc gtttgtgttc
      601 gcgtatatcg gtttggcgaa caccgccgag aaaggcgttg tgccacaaac cgtggtcgtg
      661 gcgttttatg tgggtgcggc gttgctggtg attaccagcg cgttcacgat tttcaaagtg
      721 aaggaatacg atccggaaac ctacgcccgt taccacggca tcgatgtcgc cgcgaatcag
      781 gaaaaagcca actggttcga actcttaaaa accgcgccta aagcgttttg gacggttact
      841 ctggtacagt ttttctgctg gttcgccttc caatatatgt ggacttactc ggcaggcgcg
      901 attgcggaaa acgtctggca caccaccgat gcgtcttccg taggttatca ggaggcgggc
      961 aactggtacg gcgttttggc ggcggtgcag tcggttgcgg cggtgatttg ttcgtttgta
     1021 ttggcgaaag tgccgaataa atactataag gcgggttatt tcggctgttt ggctttgggc
     1081 gcgctcggct ttttctccgt tttcttcatc ggcaaccaat acgcgctggt gttgtcttat
     1141 accttaatcg gcatcgcttg ggcgggcatt atcacttatc cgctgacgat tgtgaccaac
     1201 gccttgtcgg gcaagcatat gggcacttac ttgggcctgt ttaacggctc tatctgtatg
     1261 cctcaaatcg tcgcttcgct gttgagtttc gtgcttttcc ctatgctggg cggcttgcag
     1321 gccactatgt tcttggtagg gggcgtcgtc ctgctgctgg gcgcgttttc cgtgttcctg
     1381 attaaagaaa cacacggcgg ggtttgagcg atgagcgata cccccgctac ccccgctacc
     1441 cgcgatttcg gcctgatcga cgggcgtgcc gtaaccggct atgtgctgtc caatcggcgt
     1501 ggtacgcgtg tctgcgtgct ggacttgggc gggattgtgc aggaattttc cgttttggca
     1561 gacggcgtgc gcgaaaacct cgtggtgtcg ttcgacgatg cggcttccta tgcggacaat
     1621 ccgtttcaga ttaacaagca gatagggcgc gtggccggac gcatccgcgg tgcggcgttc
     1681 gacatcaacg gcaggactta ccgcgtggag gccaacgaag gcaggaacgc gctgcacggc
     1741 ggttcgcacg ggctggccgt tacccgtttc aacgcggtgg cggcagacgg ccgttcggtg
     1801 gtgctgcgca gccgcctgca acagtcggcc gacggttatc ccaacgattt ggatttggat
     1861 atttcctacc gcttggacga ggacgaccgg cttaccgtta cctatcgcgc caccgcgctc
     1921 ggcgacacgg tgttcgaccc gacgctgcac atttactggc ggctggacgc gggcctgcac
     1981 gatgcggttc tgcatattcc gcagggcgga catattccgg ccgatgccga aaaactgccc
     2041 gtctcaacgg tttcagacgg cctcgaagta tttgatttca gccggcccaa gccgctggat
     2101 gccgccgttg ccgccctgcg ccgcgaaacg ggtcgggccg gttttgacga cgcttaccgc
     2161 gtgccgtccg atataggccg tcccgccgct gtgttgcaag ccggacgccg ctgtcgtatc
     2221 agcatataca gcgaccgcaa cggcttggtc atctttaccg ccgccccgca ggatttcgcg
     2281 cggcacgatg cgggcgttta cgacgcgctg gcgaccgagg cgcagacgct gcccgacagc
     2341 ctgcattggc ccgagttcgg caatattcgt ctgaacaagg gtgataccag ggaggcgacg
     2401 attgcttacg gcatcgaatc cctttcttag gagcttccta acaccggttg cagacggcc
//
LOCUS       DAWHSK010000257         2454 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.257, whole genome shotgun sequence.
ACCESSION   DAWHSK010000257 DAWHSK010000000
VERSION     DAWHSK010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.257"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(119..1570)
                     /locus_tag="WIC03_001919"
     CDS             complement(119..1570)
                     /locus_tag="WIC03_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HEZ3884375.1"
                     /translation="MLSFLSGLLSLGIEVLWVRMFSFAAQSVPQAFSFTLACFLTGIA
                     VGAYFGKRICRSRFVDIPFIGQCFLWAGIADFLILGAAWLLTGFSGFVHHAGIFITLS
                     AVVRGLIFPLVHHVGTDGNKSGRQVSNVYFANVAGSALGPVLIGFVILDFLSTQQIYL
                     LICLISAAVPLFCTLFQKSLRLNAVSVAVSLMFGILMFLLPDSVFQNIADRPDRLIEN
                     KHGIVAVYHRDGDKVVYGANVYDGAYNTDVFNSVNGIERAYLLPSLKSGIRRIFVVGL
                     STGSWARVLSAIPEMQSMIVAEINPAYRSLIADEPQIAPLLQDKRVEIVLDDGRKWLR
                     RHPDEKFDLILMNTTWYWRAYSTNLLSAEFLKQVQSHLNPDGIVMFNTTHSPHAFATA
                     VHSIPYAYRYGHMVVGSATPVVFPNKELLKQRLSRLIWPESGRHVFDSSTVDAAAQKV
                     VSRMLIQMTEPSAGAEVITDDNMIVEYKYGRGI"
     gene            complement(1593..2213)
                     /locus_tag="WIC03_001920"
     CDS             complement(1593..2213)
                     /locus_tag="WIC03_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEZ3884376.1"
                     /translation="MNSTASKTLKGLSLVFFASGFCALIYQVSWQRLLFSHIGIDLSS
                     ITVIISVFMVGLGVGAYFGGRIADRFPSSIIPLFCIAEVSIGLFGLVSRGLISGLGHL
                     LVEADLPIIAAANFLLLLLPTFMMGATLPLLTCFFNRKIHNVGESIGTLYFFNTLGAA
                     LGSLAAAEFFYVFFTLSQTIALTACFNLLIAASVWLRYRKDGYSEH"
BASE COUNT          768 a          581 c          572 g          533 t
ORIGIN      
        1 ggaataagaa ttcgggacaa aatgtttaaa ccattttgtc ccgactgctg tgatgcggtt
       61 tttttgaact agcaggacgt tacctaaaac ctgcgttgcc tgaaaccctt taagacggtt
      121 aaatccctct gccgtatttg tattctacaa tcatattatc gtcggtaata acttccgccc
      181 cagccgaagg ttccgtcatc tgaatcagca tacgagagac aaccttttgt gctgcagcat
      241 ccacggtgct gctgtcaaat acgtgcctgc cgctttccgg ccaaatcaac cgggagagac
      301 gttgcttgag cagttcttta ttagggaaaa ctaccggggt tgccgagccg actaccatat
      361 gcccatagcg gtatgcatag ggaatactgt gtacggcggt agcaaaagca tgcgggctgt
      421 gcgtggtatt aaacattaca ataccatccg ggttaaggtg gctttgcacc tgttttaaaa
      481 attccgcact caacaggttg gtggaatagg cacgccagta ccaagtcgta ttcatcaaaa
      541 tcaggtcgaa tttttcatca ggatggcgac gcagccattt cctaccgtca tccaatacaa
      601 tttcaacacg tttgtcctgc aaaagcgggg cgatttgcgg ctcgtccgcg ataaggctac
      661 ggtatgccgg attgatttcc gcaacgatca tcgactgcat ttccggaatg gcagacaaga
      721 cgcgcgccca cgaacctgta ctcaatccaa cgacgaaaat gcggcgtatg ccagacttca
      781 gggagggtag cagataggca cgttcgatgc cgttgacact attgaataca tcggtattgt
      841 atgcgccgtc gtatacattc gccccataaa caaccttatc accatctcta tggtaaaccg
      901 caacaatgcc gtgtttgttt tcaatcagcc tatccggacg gtcagcaata ttttgaaaga
      961 cagaatccgg cagtaggaac atgaggatgc cgaacattag ggaaactgct accgacactg
     1021 cattcagtcg gagacttttt tggaacagtg tacaaaacaa agggacagca gcagaaatca
     1081 aacagatgag caggtaaatc tgttgggtgg acaagaaatc aagtatcaca aagccgataa
     1141 ggaccggacc caatgcactg ccggcaacgt tggcgaaata aacattggaa acctgtcgtc
     1201 cggatttgtt gccatccgta cccacatggt gtacgagcgg gaaaatcaac cctctgacga
     1261 cggcagacag ggtaatgaag ataccggcgt ggtggacgaa gccggaaaaa cccgtcaaca
     1321 accacgcagc acccaaaatc aaaaagtcgg caatacccgc ccacaagaag cactgcccga
     1381 taaagggaat atcaacaaag cggctgcggc aaatccgttt gccaaaatac gcgccgacgg
     1441 cgataccggt cagaaaacag gcaagggtaa atgaaaatgc ctgaggcacg gactgtgctg
     1501 cgaacgaaaa catcctcacc cacaagactt ctatacccaa gctcaataag ccgctaagga
     1561 aagaaagcat ataaatcaaa ctagtattcg gtttagtgtt cactatatcc atcctttctg
     1621 taacgcagcc atactgaagc agcaatcaga aggttaaagc aggctgtcag cgcaatggtt
     1681 tgggagaggg taaaaaagac gtagaaaaat tcggcggcgg caagcgatcc gagtgccgca
     1741 cccaaagtgt tgaaaaaata taaggtaccg atagactcgc caacattatg tattttccgg
     1801 ttaaaaaaac aggtcagcaa gggcaaggtc gcgcccatca taaaggtagg aagcagcaat
     1861 aagaggaaat tggcagcagc gatgatgggc aaatcagcct caactaaaag atgccccaag
     1921 ccggaaatca gacccctgct taccaaaccg aacagaccga tggatacttc agcgatgcaa
     1981 aacaggggga tgatacttga aggaaaacgg tcagcaatgc gtccaccgaa atacgcacct
     2041 acacccaagc cgaccataaa tacagaaata atgacagtaa tcgaactcaa atcgatacct
     2101 atgtgactga atagaagcct ctgccagctg acctggtaaa tcagggcgca gaatccagag
     2161 gcgaaaaaca ccagcgacaa tcctttcagg gttttacttg cggtcgaatt catattttct
     2221 attttcaaat aggcggaact ataaatgaaa tgtatcaatg tgtaaacatt tgtattaacc
     2281 gtaggaaatc accgtattgc tcaaactgca ggccgggaaa ataaactcgt aagcccgcaa
     2341 acggcgtatc actgttcaaa ctgctgtgcg gcttcacggt gttataaaaa taatggggct
     2401 gtcctggaca actaagataa actcgatttt actaattgtt ttaaaatgga aatt
//
LOCUS       DAWHSK010000258         2453 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.258, whole genome shotgun sequence.
ACCESSION   DAWHSK010000258 DAWHSK010000000
VERSION     DAWHSK010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.258"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            212..1276
                     /locus_tag="WIC03_001921"
     CDS             212..1276
                     /locus_tag="WIC03_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-butanediol dehydrogenase"
                     /protein_id="HEZ3884377.1"
                     /translation="MKAARFYDKGDIRIEDIPEPTVAPGTVGINVAWCGICGTDLHEF
                     MEGPIFIPPCGHPHPISGESAPVTMGHEFSGVVYAVGEGVDDIKVGQHVVVEPYIIRD
                     DVPTGEGSNYHLSKDMNFIGLGGCGGGLSEKIAVKRRWVHPISDKIPLDQAALIEPLS
                     VGHHAYVRSGAKAGDVALVGGAGPIGLLLAAVLKAKGIKVIITELSKARKDKARESGV
                     ADYILDPSEVDVVEEVKKLTNGEGVDVAFECTSVNKVLDTLVEACKPAANLVIVSIWS
                     HPATVNVHSVVMKELDVRGTIAYCNDHAETIKLVEEGKINLEPFITQRIKLDELVSEG
                     FERLIHNNESAVKIIVSPNL"
     gene            1417..1740
                     /locus_tag="WIC03_001922"
     CDS             1417..1740
                     /locus_tag="WIC03_001922"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HEZ3884378.1"
                     /translation="MGDSVLSAIQQTITQRKSANPSESYVAQLLHKGEDKILKKVIEE
                     AGEVLMASKDKNPSHLVYEVADLWFHTMILLTHHDLKAEDVLDELARRQGLSGLAEKA
                     ARTES"
     gene            1812..2135
                     /locus_tag="WIC03_001923"
     CDS             1812..2135
                     /locus_tag="WIC03_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEZ3884379.1"
                     /translation="MDNCIFCKIAAKDIPAQTVYEDGEMVCFKDINPAAPVHLLLIPK
                     VHFDSLAHAAPEHQPLLGKMMLKVPEIAKAAGLADGFKTLINTGKGGGQEVFHLHIHI
                     MGTPV"
     gene            2182..2385
                     /gene="tatA"
                     /locus_tag="WIC03_001924"
     CDS             2182..2385
                     /gene="tatA"
                     /locus_tag="WIC03_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HEZ3884380.1"
                     /translation="MGSFSLTHWIIVLIIVVLIFGTKKLRNVGKDLGGAVHDFKQGLN
                     EGTDGKEAQKDDVIEHKKDEDKA"
     gene            2389..>2453
                     /locus_tag="WIC03_001925"
     CDS             2389..>2453
                     /locus_tag="WIC03_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatB"
                     /protein_id="HEZ3884381.1"
                     /translation="MFDFGLGELVFVGIIALIVLGP"
BASE COUNT          670 a          679 c          583 g          521 t
ORIGIN      
        1 atgccgtctg aagccgattc ggttttcaga cgacatcatt atttgacctg acgcaaacag
       61 aaagccttcc gcgtgcttaa acacatctct tgtagtaaaa tatctaaaca gattcaactg
      121 gaaacggtca ggcttcttca cgccattccc atttcaaccg ccgcctgcca gattcagacg
      181 gtattcctac ttgaaaacaa ggagcgttac aatgaaagca gcacgttttt acgataaagg
      241 cgacatccgc atcgaagaca tccccgaacc gaccgtcgcc cccggcactg tcggcatcaa
      301 tgtcgcctgg tgcggcatct gcggtactga cctgcacgaa ttcatggaag gcccgatttt
      361 cattccgcct tgcggtcatc cgcacccgat ttccggcgag tccgcacccg taacgatggg
      421 acacgagttc tccggcgtgg tctatgccgt cggcgaaggc gtggacgaca tcaaagtcgg
      481 ccaacacgtc gtggtcgaac cctacatcat ccgcgatgac gtaccgaccg gagaaggcag
      541 caactaccac ctctccaaag atatgaactt tatcggcttg ggcggctgcg gcggcggtct
      601 gtccgaaaaa atcgccgtca aacgtcgttg ggtgcatccg atttccgaca aaatcccgtt
      661 ggatcaagcc gctttgatcg aaccgctgtc tgtcggacac cacgcctatg tacgcagcgg
      721 cgcgaaagca ggcgacgtcg cattggtcgg cggcgcaggt ccgatcggtt tgctgttggc
      781 tgccgtgttg aaagccaaag gcatcaaagt catcatcacc gagttgagta aagcacgcaa
      841 agacaaagcg cgcgaatccg gcgttgccga ctacatcctc gacccgtccg aagtcgatgt
      901 tgtcgaagaa gtgaaaaaac tgaccaacgg cgaaggcgtg gacgtggcat tcgaatgtac
      961 cagcgtgaac aaagtgttgg atactttggt cgaagcctgc aaacctgccg ccaatttggt
     1021 tatcgtatcc atctggagcc accctgcaac cgtcaacgtc cacagcgtcg tgatgaaaga
     1081 gctggatgtg cgcggcacga ttgcctactg taacgaccac gccgaaacca tcaaactggt
     1141 cgaagaaggc aaaatcaacc tcgaaccctt catcacccag cgcatcaagc tggacgaact
     1201 ggtttccgaa ggcttcgagc gtctgatcca caacaacgaa tccgccgtta aaattattgt
     1261 gagtccaaac ctgtaagcaa attacatttg caacagcaca aatgccgtct gaaacgctca
     1321 gacggcattt tccaacgaag cgcaacaggc gtattgcccg agagcagccg tttagcctat
     1381 cattccgaac aaacagaaac aaacagaagg aaaatcatgg gagattccgt actatccgcc
     1441 atccaacaaa ccatcaccca gcgaaaatct gccaatccgt ccgaatctta cgtcgcacag
     1501 ctcttgcata agggcgaaga caaaatccta aaaaaagtga ttgaagaggc gggcgaagtg
     1561 ttgatggcat ccaaagacaa aaacccgtcc cacctggttt acgaagttgc cgacttatgg
     1621 tttcacacca tgattcttct gacacaccac gacctgaagg cggaagacgt attggacgaa
     1681 cttgcgcgcc gccaaggttt gtcgggcttg gccgaaaaag ccgctcgcac agaatcttga
     1741 atttatatta aaatccgcac tttcccacat tcaatccgtc tgaccgctgt tcagacggca
     1801 tcggagccgt tatggacaac tgtattttct gcaaaatcgc cgccaaagac attccggcgc
     1861 aaaccgtcta tgaagacggc gaaatggttt gtttcaaaga catcaacccc gctgctccgg
     1921 ttcatctgct gctgattccc aaagtccatt tcgattcgtt ggcacacgcc gcgcccgaac
     1981 atcagcccct tttgggaaaa atgatgctga aagttcccga aatcgccaaa gcggcaggac
     2041 tggcagacgg cttcaaaacc ctgatcaaca ccggaaaagg cggcggacaa gaggtcttcc
     2101 acctgcatat acacatcatg ggcacacccg tataaaccgt tatttcacaa tcaaccccta
     2161 atacttactt aaggatacat catgggcagt ttttctctga cgcactggat tatcgtactg
     2221 attatcgtcg ttttgatatt cggcaccaaa aaactgcgca acgtcggcaa agacctcggc
     2281 ggtgcggttc atgacttcaa acaggggctg aacgaaggta cagacggcaa agaagcccaa
     2341 aaagacgatg taatcgaaca caaaaaagac gaagacaaag cgtaatttat gtttgatttc
     2401 ggtttgggcg agctggtttt tgtcggcatt atcgccctga ttgtcctcgg ccc
//
LOCUS       DAWHSK010000259         2421 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.259, whole genome shotgun sequence.
ACCESSION   DAWHSK010000259 DAWHSK010000000
VERSION     DAWHSK010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.259"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            59..1227
                     /locus_tag="WIC03_001926"
                     /pseudo
     CDS             59..1227
                     /locus_tag="WIC03_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742687.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            1272..1562
                     /locus_tag="WIC03_001927"
     CDS             1272..1562
                     /locus_tag="WIC03_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884382.1"
                     /translation="MNIQLQGHIVGVKKFNGQIEGRSFDYCRLIVATPLDSSQGNALG
                     SSTTEYDFGGSANFEQFRNAQFPIEANLNVEIVTTGKTQKLKVIGFQLVKKG"
     gene            1567..1764
                     /locus_tag="WIC03_001928"
     CDS             1567..1764
                     /locus_tag="WIC03_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884383.1"
                     /translation="MQKVYVVQSVSTGDFLYLSPETGDIGHTKLITNADYFYDFEEAI
                     NAGLEEIGNQYEFVVFGFLKD"
     gene            1772..2056
                     /locus_tag="WIC03_001929"
     CDS             1772..2056
                     /locus_tag="WIC03_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884384.1"
                     /translation="MFGGRLKNAPSITAKHFLKENIMKFINTCRKYGAKLAVVTAVPL
                     ALATQANATLPDTAKNALEAAKADGMEAGWIVVGIFAALFVFSIVKRVMK"
     gene            2063..2341
                     /locus_tag="WIC03_001930"
     CDS             2063..2341
                     /locus_tag="WIC03_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884385.1"
                     /translation="MYYQVGNKCLEKHQAENLYFSLVVPRIKENGQIVRPEYNGSLWK
                     MSDGQPLRLLLAECSPKDNLQSGLETGWIVFGVMASVYFVSLLKKVLK"
BASE COUNT          717 a          548 c          564 g          592 t
ORIGIN      
        1 gacagaaacc gaaacggcag gcagggttca ggaagtcttc gaatgttacg aaacgtacat
       61 aacggacggc aaaggaaacc tgttaggcgt tcctcttcgg cgcggtgtat cagattcggc
      121 tttcattgat caaattagct tttcatttca tgaaaaaccc tttttcgata aatacggcgt
      181 tcgtgtaagt cttttggaag acgaagattt tattcgcgcc gcgtccatgc tcgccgaaga
      241 agttttcggt ttcggtatct acaaagagtc caaaggctcg ggcggccgtt tttatgagcg
      301 ctgttggttg atgggttcgg aagacgtcct atacggtcgc gtccattttg gcggacaaaa
      361 caataccatt cttttcgaac tgaccggcac cggttgcggc gtcgcaaaag aaggctggga
      421 atcacgactt ttcgcattcc tgactaatgc aatccgccca aaaatcacac gcgttgacat
      481 cgcaaaagac tttttcaacg gcgaatacag cccgaaccaa gcccgtgaag accgaaataa
      541 aggtatgttt acctgtcatc acgtcaaacc aaaaggcgaa tgtttggggt cagattggga
      601 agaagacgat gaagccaaaa tgaccaaagg caagacctat ggtatcggct cccgtgaatc
      661 gtccaaatat gtccgcgtct atgaaaaagg caagcagttg ggcgataaaa caagcacatg
      721 gacgcgcttt gaaattgaat tcaaagcaaa agacatcgtt atcccttttg aagttttgca
      781 gaatccgggc gaatatttcg gcggcgcata tccgatttgc gaacgattcg tccaaaaggc
      841 aacgcgcata cacgcggtta aggaagataa ggtcatttca gccgaccgtt accttgaatg
      901 ggtaaaaaac agttcggacg tgcggcaaac ggtctgaaat tcatttttcc cgaattggac
      961 aaagccaaac tgtttgaact gattgagccg agtcatcaca agctgcccaa gtctttggct
     1021 cccgaagcct acgactgcgc ctttttgaaa gctcaagcca ttcatgaaca gcccgcattc
     1081 aaaccgtaca atgaccctta ctatatgtac gaatattacg agaatcttga aaaacagctt
     1141 gaacagcaaa aacacgtcaa caatgaagaa agctataaca acttcattta cgacaaattc
     1201 gcaagactac cgatttcatg ggcttaaagt gtctgcccga aagacgttta atcacacaag
     1261 gaaaccaaaa aatgaacatc caacttcaag gccacatcgt cggcgttaaa aaattcaacg
     1321 gacaaatcga aggcaggagc ttcgactatt gccgcctgat tgtcgccaca cccttagaca
     1381 gctcccaagg caacgcattg ggcagctcta ctactgaata cgatttcggc ggctctgcca
     1441 atttcgagca gttccgaaac gcccaatttc cgatcgaagc aaacctgaac gtagaaatcg
     1501 ttactacggg caaaacccaa aaactgaaag tcatcggttt tcaactcgtg aagaaaggct
     1561 gattgaatgc agaaagtcta tgttgtccag tccgtatcaa caggggactt tctgtatctc
     1621 tctcctgaaa cgggcgacat cggacatacc aaattaatca ccaatgccga ttatttctac
     1681 gacttcgaag aagcgattaa cgcaggtttg gaagaaatcg gcaaccaata cgaatttgtc
     1741 gtattcggat ttttgaaaga ctgattttcg gatgttcggc ggtcgtctga aaaacgctcc
     1801 atccattacc gccaaacact ttttgaagga aaatatcatg aaatttatta acacctgccg
     1861 taaatacggc gcaaaactgg ctgttgtaac agctgtcccg ctggctttgg cgacacaggc
     1921 aaatgcaacg ttgcccgata cggcaaaaaa cgctttggaa gccgcaaaag ccgacggtat
     1981 ggaagccggt tggattgtag tgggcatttt cgccgcgctt tttgtctttt ccatcgttaa
     2041 gagagtgatg aagtaagacg gcatgtacta ccaagtcgga aataaatgtc ttgagaagca
     2101 ccaggctgaa aacctttatt tcagcttggt agtaccaaga atcaaagaaa acggacagat
     2161 tgtcagaccg gaatataacg gcagtctgtg gaagatgtcg gacggtcagc cgctaaggct
     2221 tttattggcg gaatgcagtc cgaaagacaa cctgcaaagc ggtcttgaaa caggctggat
     2281 agtattcggc gtgatggcgt ccgtttactt tgtttccctg ctgaaaaagg ttttgaaatg
     2341 atggattttt atttttatct cggcgtttcc gtacccgtat taatcggggc ggttctgttt
     2401 aagaattgag cgcatgaagt t
//
LOCUS       DAWHSK010000260         2417 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.260, whole genome shotgun sequence.
ACCESSION   DAWHSK010000260 DAWHSK010000000
VERSION     DAWHSK010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.260"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            52..2202
                     /gene="yccS"
                     /locus_tag="WIC03_001931"
     CDS             52..2202
                     /gene="yccS"
                     /locus_tag="WIC03_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HEZ3884386.1"
                     /translation="MKTPLLKPLLITSLPVFASVFTAASIVWQLGEPKLAMPFVLGII
                     AGGLVDLDNRLTGRLKNIITTVALFTLSSLTAQSTLGTGLPFILAMTLMTFGFTILGA
                     VGLKYRTFAFGALAVATYTTLTYTPETYWLTNPFMILCGTVLYSTAIILFQIILPHRP
                     VQESVANAYDALGGYLEAKADFFDPDEAAWIGNRHIDLAMSNTGVITAFNQCRSALFY
                     RLRGKHRHPRTAKMLRYYFAAQDIHERISSAHVDYQEMSEKFKNTDIIFRIHRLLEMQ
                     GQACRNTAQALRASKDYVYSKRLGRAIEGCRQSLRLLSDSNDNPDIRHLRRLLDNLGS
                     VDQQFRQLQHNGLQAENDRMGDTRIAALETGSLKNTWQAIRPQLNLESGVFRHAVRLS
                     LVVAAACTIVEALNLNLGYWILLTALFVCQPNYTATKSRVRQRIAGTVLGVIIGSLVP
                     YFTPSVETKLWIVIASTTLFFMTRTYKYSFSTFFITIQALTSLSLAGLDVYAAMPVRI
                     IDTIIGASLAWAAVSYLWPDWKYLTLERTAALAVCSNGAYLEKITERLKSGETGDDVE
                     YRATRRRAHEHTAALSSTLSDMSSEPAKFADSLQPGFTLLKTGYALTGYISALGAYRS
                     EMHEECSPDFTAQFHLAAEHTAHIFQHLPETEPDDFQTALDTLRGELDTLRTHSSGTQ
                     SHILLQQLQLIARQLEPYYRAYRQIPHRQPQNAA"
BASE COUNT          582 a          933 c          480 g          422 t
ORIGIN      
        1 ggtttttgtt aatccactat attcacttca taacaataaa ccggtaaaac catgaaaacc
       61 ccactcctca agcctctgct cattacctcg cttcccgttt tcgccagtgt ttttaccgcc
      121 gcctccatcg tctggcagct aggcgaaccc aagctcgcca tgcccttcgt actcggcatc
      181 atcgccggcg gccttgtcga tttggacaac cgcctgaccg gacggctgaa aaacatcatc
      241 accaccgtcg ccctgttcac cctctcctcg ctcacggcac aaagtaccct cggcacaggg
      301 ctgcctttca tcctcgccat gaccctgatg accttcggct ttaccatttt aggcgcggtc
      361 gggctgaaat accgcacctt cgccttcggc gcactcgccg tcgccaccta caccacactt
      421 acctacaccc ccgaaaccta ctggctgacc aaccccttca tgattttatg cggcaccgta
      481 ctgtacagca ccgccatcat cctgttccaa atcatcctgc cccaccgccc cgtccaagaa
      541 agcgtcgcca acgcctacga cgcactcggt ggctacctcg aagccaaagc cgacttcttc
      601 gaccccgatg aggcagcctg gataggcaac cgccacatcg acctcgccat gagcaacacc
      661 ggcgtcatca ccgccttcaa ccaatgccgt tccgccctgt tttaccgcct tcgcggcaaa
      721 caccgccacc cgcgcaccgc caaaatgctg cgctactact tcgccgccca agacatacac
      781 gaacgcatca gctccgccca cgtcgactac caagagatgt ccgaaaaatt caaaaacacc
      841 gacatcatct tccgcatcca ccgcctgctc gaaatgcagg gacaagcctg ccgcaacacc
      901 gcccaagccc tgcgcgcaag caaagactac gtttacagca aacgcctcgg ccgcgccatc
      961 gaaggctgcc gccagtcgct gcgcctcctt tcagacagca acgacaatcc cgacatccgc
     1021 cacctgcgcc gccttctcga caacctcggc agcgtcgacc agcagttccg ccaactccag
     1081 cacaacggcc tgcaggcaga aaacgaccgc atgggcgaca cccgcatcgc cgccctcgaa
     1141 accggcagcc tcaaaaacac ctggcaggca atccgtccgc agctaaacct cgaatcaggc
     1201 gtattccgcc atgccgtccg cctgtccctt gtcgttgccg ccgcctgcac catcgtcgaa
     1261 gccctcaacc tcaacctcgg ctactggata ctactgaccg cccttttcgt ctgccaaccc
     1321 aactacaccg ccaccaaaag ccgcgtccgc cagcgcatcg ccggcaccgt actcggcgta
     1381 atcatcggct cgctcgtccc ctactttacc ccctccgtcg aaaccaaact ctggatcgtc
     1441 atcgccagta ccaccctctt tttcatgacc cgcacctaca aatacagctt ctcgacattt
     1501 ttcatcacca ttcaagccct gaccagcctc tccctcgcag ggttggacgt atacgccgcc
     1561 atgcccgtac gcatcatcga caccattatc ggcgcatccc ttgcctgggc ggcggtcagc
     1621 tacctatggc ccgactggaa atacctcacg ctcgaacgca ccgccgccct tgccgtatgc
     1681 agcaacggcg cctatctcga aaaaatcacc gaacgcctca aaagcggcga aaccggcgac
     1741 gacgtcgaat accgcgccac ccgccgccgc gcccacgaac acaccgccgc cctcagcagt
     1801 accctttccg acatgagcag cgaacccgca aaattcgccg acagcctgca acccggcttt
     1861 accctgctca aaaccggcta cgccctgacc ggctacatct ccgccctcgg cgcataccgc
     1921 agcgaaatgc acgaagaatg cagccccgac tttaccgcac agttccacct cgccgccgaa
     1981 cacaccgccc acatcttcca acacctgccc gaaaccgaac ccgacgactt tcagacagca
     2041 ctggatacac tgcgcggcga actcgacacc ctccgcaccc acagcagcgg aacacaaagc
     2101 cacatcctcc tccaacagct ccaactcatc gcccgacagc tcgaacccta ctaccgcgcc
     2161 taccgacaaa ttccgcacag gcagccccaa aacgcagcct gaaaaagttt cggcattttg
     2221 taagagaggg cagattgtca gacaggttgc aagatagtgg ataaagcttt gcgcagggta
     2281 aatgcgtagc aactgaaccg tcattcccac gaacctacat cccgtcattc ccacgaaaac
     2341 agaaaaccaa aaacagaaac ctaaaatccc gtcattccca cgaaagtggg aatccagaat
     2401 cccggacttt cagataa
//
LOCUS       DAWHSK010000261         2410 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.261, whole genome shotgun sequence.
ACCESSION   DAWHSK010000261 DAWHSK010000000
VERSION     DAWHSK010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.261"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..868
                     /locus_tag="WIC03_001932"
     CDS             <1..868
                     /locus_tag="WIC03_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HEZ3884387.1"
                     /translation="AIIALAVTVVTAGAGVGAALGLNGAAAAAADAAFASLASQASVS
                     LINNKGDVGKTLKELGRSRTVKNLVVAAATAGVSNKLGASSLATWSETPWVNNLNVNL
                     ANAGSAALINTAVNGGSLKDNLEANILAALVNTAHGEAASKIKQLDQHYIAHKVAHAV
                     AGCAAAAANKGKCQDGAIGAAVGEILGEALLDGRDPGSLNVKDRAKIIAKAKLAAGTV
                     AALSKGDVNAAANAAAVAVESNALSKERMDKLTKCLSGKTCSTTMEKVNAIKKDEQFS
                     KVIDTEIQKSLF"
     gene            876..1571
                     /locus_tag="WIC03_001933"
                     /pseudo
     CDS             876..1571
                     /locus_tag="WIC03_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238820.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
     gene            1559..2002
                     /locus_tag="WIC03_001934"
     CDS             1559..2002
                     /locus_tag="WIC03_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884388.1"
                     /translation="MQAMNKNYLFYLIQNIKSIFLIGGVFFGLLIFSGLWGNDFKLSF
                     LGVIDFIFGMVGGGFIVMLWFLIPVILSTLFRWVFQNQLAVSVILTFIVFYTYGYFSL
                     FNSSERVGSFWYLVIPTIMFIGLSEWFIYKSSVHSLGENVTPRSN"
     gene            complement(2070..2338)
                     /locus_tag="WIC03_001935"
                     /pseudo
     CDS             complement(2070..2338)
                     /locus_tag="WIC03_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244123.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT          692 a          429 c          594 g          695 t
ORIGIN      
        1 agcgattatc gcgctggctg ttaccgtggt tactgcgggc gcgggagtcg gagccgcact
       61 aggcttaaac ggcgcagccg cagcagcggc cgatgccgcc tttgcctcac tcgcttctca
      121 ggcttccgta tcgctcatca acaataaagg cgatgtcggc aaaaccctga aggaactggg
      181 cagaagccgc acggtaaaaa atctggttgt agcggcggca acggcaggcg tatccaacaa
      241 actcggtgcc tcttcccttg ccacttggag cgaaacccct tgggtaaaca acctcaacgt
      301 taacctggcc aatgcgggca gtgccgcgct gatcaacacc gctgttaacg gcggcagcct
      361 gaaagacaat ctggaggcaa atatcctggc ggcattggtg aataccgcgc atggggaggc
      421 tgcgagtaag attaagcaat tggatcagca ctatatcgcc cacaaagttg cccatgccgt
      481 agcaggctgt gcagctgcgg cggcgaataa gggcaagtgt caggacggcg cgataggagc
      541 ggcggtcggt gaaatcctcg gcgaagccct actggacggc agagaccctg gcagcctgaa
      601 tgtgaaggac agggcaaaaa tcattgctaa ggcgaagctg gcagcaggga cggttgcggc
      661 gttgagcaag ggggatgtga atgctgcggc gaatgcggct gctgtggcgg tagagagtaa
      721 tgcgcttagc aaggaaagaa tggataaatt gacaaaatgc ctttccggta aaacttgttc
      781 tactacgatg gaaaaagtaa atgccatcaa aaaggatgaa caatttagca aagtaattga
      841 tacggaaatt caaaaaagtc tgttctagga acccattggg cgatggttgc agaaacggca
      901 ttaatatgtc tattaaatat attgccatgc ctgctgcgtg gaagtatatg cctacggatg
      961 tatcacgggt tgccaaagaa gtttttggct atttatataa ctcacaaggg gcatctacaa
     1021 gatttgacaa gtatttcaac accattgaca atcgtgcaga tttctttgct gccagcaatc
     1081 tttatgagca aaatttgggt tcaaaagcac gatggtttgg tggagctgat tttgtatcgc
     1141 gtgctgctat aactgggtta ggggcagacg gagaagcttc ttatataact tttgcggcag
     1201 gtaaagttgt tggtaatcct ccaatttatg aatggagggc tgcgtcaggc aatgcactga
     1261 tagtaaatgg attttataat tttagagact tgttcaataa aaaaactaat cctagggagt
     1321 gggatattca acagttgaaa agcgagcaaa agttattaca gcctattcaa ccaaaaatat
     1381 ttgagtaatg agaaggatta tttgtcattg attaaagggg taacatcaaa taagatattt
     1441 tcaataattc caaatccttt agatgagagg aaaaaataga ggatggaatt aatatgttag
     1501 attacaaatc tagaattaaa tatggttgta agcttatggg atattctgag aaacagggat
     1561 gcaagccatg aataaaaatt atttatttta tcttattcaa aatattaaat ctattttctt
     1621 aataggtgga gtattttttg gccttttaat tttttcaggt ttatggggta atgattttaa
     1681 actatctttt ttaggggtaa tagattttat atttgggatg gtagggggtg ggtttatagt
     1741 tatgttatgg tttctaattc cagtgatatt atcaacttta ttcagatggg tatttcaaaa
     1801 tcaacttgct gtatctgtca tattaacctt cattgttttt tatacatatg ggtacttttc
     1861 tttatttaat tcatctgaaa gagtcggtag tttttggtat ttggtaatcc caaccataat
     1921 gtttattgga ctgtctgaat ggtttatcta taaatctagc gtgcatagtt taggtgaaaa
     1981 tgtaactcca cgttccaatt gaagaaaaag attgtctgaa aactaaattt aatttcagat
     2041 gaccttagat tcggatttca agtacaacac tagggtatcg atggttggaa caaattcaag
     2101 aataaaacac ttggcgtttc gtagccaagt gtttttctcg gtcggtggtt caactcatct
     2161 tgaaccctac gtatctcccg atcgctgatg tttcggaaaa cggtttgttt ggggaatatt
     2221 gtcggatgag tccattggtg ttttcattca gccctttctc ccaagaatgg taagggcggt
     2281 aaaaataggt ttccgccttc aatgttttgg ccatttggat gtgtcggtag aattctttga
     2341 ttaaacaagt tgcaaataac ttacctgatg gtagtcctgc taaagctgct gtcttcaaag
     2401 cggagcggga
//
LOCUS       DAWHSK010000262         2409 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.262, whole genome shotgun sequence.
ACCESSION   DAWHSK010000262 DAWHSK010000000
VERSION     DAWHSK010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.262"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..705
                     /locus_tag="WIC03_001936"
     CDS             <1..705
                     /locus_tag="WIC03_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEZ3884389.1"
                     /translation="TDAAEQTESRRQNAKIAKDARKLLEQKGNEQQLNKLLSNLEKKK
                     AEHRIQDAEAKRKLAEARLAAAEKARKEAAQQKAEARRAEMSNLTAEDRNIQAPSVMG
                     IGSADGFSRMQGRLKKPVDGVPTGLFGQNRSGGDVWKGVFYSTAPATVESIAPGTVSY
                     ADELDGYGKVVVVDHGENYISIYAGLSEISVGKGYMVAAGSKIGSSGSLPDGEEGLYL
                     QIRYQGQVLNPSSWIR"
     gene            825..2309
                     /locus_tag="WIC03_001937"
     CDS             825..2309
                     /locus_tag="WIC03_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEZ3884390.1"
                     /translation="MSKPVFKKIALYTLGAISGVAVSLAVQGFAAEKDRRDNEVLPVQ
                     SIRTMAEVYGQIKANYYQDKPDADLFEGAMKGMVAGLDPHSEYMDKKGYAEIKESTSG
                     EFGGLGMEIGQEDGFVKVVSPIEDTPAERAGVKSGDFIVKIDNVSTRGMTVSEAVKKM
                     RGKPGTKITLTLSRKNADKPIVVNLTRAIIKVKSVRHHLIEPDYGYIRVSQFQERTVE
                     SVNTAAKELLKENKGKPLKGLVLDLRDDPGGLLTGAVGVSAAFLPSEAVVVSTKGRDG
                     KDRMVLKAIPEDYVYGMGGDSLAGIPAELKTIPMTVLVNSGSASASEIVAGALQDHKR
                     AVIVGTQSFGKGSVQTLIPLSNGSAVKLTTALYYTPNDRSIQAQGIVPDVEVKDKERI
                     FESREADLVGHIGNPLGGEDVNSETLAVPLEKDADKPAVKEKGKKKKDEDLSSRRIPN
                     PAKDDQLRKALDLVKSPEQWQKSLGLAAKKPVSNKDKKDKKDKK"
BASE COUNT          653 a          527 c          739 g          490 t
ORIGIN      
        1 accgatgcgg cggaacagac ggaaagccgc agacagaatg ccaaaatcgc caaagatgcc
       61 cgaaaactgc tggaacagaa agggaacgag cagcagctga acaagctctt gagcaatttg
      121 gagaagaaaa aggccgaaca ccgcattcag gatgcggaag caaaaagaaa attggctgaa
      181 gccagactgg cggcagccga aaaagccaga aaagaagcgg cgcagcagaa ggctgaagca
      241 cgacgtgcgg aaatgtccaa cctgaccgcc gaagacagga acatccaagc gccttcggtt
      301 atgggtatcg gcagtgccga cggtttcagc cgcatgcagg gacgtttgaa aaaaccggtt
      361 gacggtgtgc cgaccggact tttcgggcag aaccggagcg gcggcgatgt ttggaaaggc
      421 gtgttctatt ccactgcacc ggcaacggtt gaaagcattg cgccgggaac ggtaagctat
      481 gcggacgagt tggacggcta cggcaaagtg gtcgtggtcg atcacggcga gaactacatc
      541 agcatctatg ccggtttgag cgaaatttcc gtcggcaagg gttatatggt cgcggcagga
      601 agcaaaatcg gctcgagcgg gtcgctgccg gacggggaag aggggcttta cctgcaaata
      661 cgttatcaag gtcaggtatt gaacccttcg agctggatac gttgatcgga ttgccgaaac
      721 ggtaggcttt atgcaaatcg tgccgatatg gtgcgctgtt ccgtgaagtt tgccgtacct
      781 gtattttccg gtttttggaa atcaagagag aaagttaaag aagaatgtcg aaacctgttt
      841 ttaagaaaat cgcactttat actttgggtg caatcagcgg cgtggccgtc agtctggcgg
      901 tgcagggttt tgccgccgag aaggacaggc gggataacga agtcctgccg gtgcaatcca
      961 tccgcacaat ggcggaggtt tacggtcaaa tcaaggcaaa ctactatcag gacaaacccg
     1021 atgccgattt gtttgaaggt gcgatgaagg gtatggtggc cggtttggat ccgcattccg
     1081 aatatatgga taaaaaaggt tatgccgaga taaaggagtc caccagcggc gaatttggcg
     1141 gcttggggat ggaaatcggg caggaagacg gatttgtcaa agtggtttcg ccgattgagg
     1201 acacgcctgc ggaacgggcg ggggtgaaaa gcggcgattt cattgtgaaa atcgataatg
     1261 tttcgacacg cggcatgacg gtcagcgaag cggtgaagaa aatgcggggc aagccgggta
     1321 cgaagattac tttgacgctg tcgcgcaaaa atgccgacaa gccgatagtc gtcaacctga
     1381 cccgtgccat tattaaagtg aaaagcgtcc gccatcacct gatcgaaccc gattacggct
     1441 atatccgcgt gtcgcagttc caagagcgga cggtcgaaag cgtcaatacc gccgcaaaag
     1501 agctgctaaa ggaaaataaa ggaaaaccgc tcaaggggct ggtgttggat ttgcgcgacg
     1561 accccggcgg gcttttgact ggcgcggtcg gcgtgtcggc ggcatttctg ccgtctgaag
     1621 cagtcgtcgt cagcaccaag ggacgcgacg gcaaagaccg catggtactg aaagccattc
     1681 ctgaagatta tgtgtacggg atgggcggcg attcgttggc gggcattcct gccgagttga
     1741 aaaccatacc gatgacggta ttggtcaatt ccggttcggc ttccgcgtcg gagattgtcg
     1801 caggtgcatt gcaggatcat aaacgcgcgg tcatcgtcgg tacgcagagc ttcggcaaag
     1861 gttcggttca gactttgatt cctttgtcca acggcagcgc ggtcaagctg acaacggcac
     1921 tgtattatac gccgaacgac cgttctattc aggcgcaggg gattgttccc gatgtcgaag
     1981 taaaagataa ggaacgcatt tttgaaagcc gcgaggcgga tttggtcgga cacatcggca
     2041 atcctttggg cggcgaggat gtgaacagtg aaacccttgc cgtgccgctt gaaaaagatg
     2101 cggataagcc cgctgtaaaa gaaaaaggta aaaagaaaaa ggacgaggat ttgtcttcaa
     2161 ggcggattcc caaccctgcc aaagacgacc agttgcggaa agctttggat ttagtcaagt
     2221 cgcccgagca gtggcagaag tctttggggc tggcggcgaa aaagccggtt tcaaataaag
     2281 ataagaaaga taaaaaagat aagaagtagg ttttgacgat gccgtctgaa aggatttcag
     2341 acggcatatt atgtttgaaa ggacaggtta tgagcgattt tttaccggaa tttgaaccgt
     2401 gcggcattt
//
LOCUS       DAWHSK010000263         2379 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.263, whole genome shotgun sequence.
ACCESSION   DAWHSK010000263 DAWHSK010000000
VERSION     DAWHSK010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.263"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..372
                     /locus_tag="WIC03_001938"
     CDS             <1..372
                     /locus_tag="WIC03_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HEZ3884391.1"
                     /translation="RLRRLVLIFVNPLYIIATTVPAQQAFLDAAELMQWSIETEGLGL
                     NVISHKILGKDHAQVEFEAYFRDGQHRSAHHELSGFVNIGGQWYFIDPTVPHPAMKQP
                     CICGSGKKFKACCGKYLKPVA"
     gene            complement(591..1385)
                     /locus_tag="WIC03_001939"
     CDS             complement(591..1385)
                     /locus_tag="WIC03_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEZ3884392.1"
                     /translation="MLHRLQKVVRHIAQTEIMPRFLNTPSHRKEDGSMLSEADIAAQT
                     AFAAALPLLIDCPMLGEEMSRQEQSALWEQYSGEKGLWIVDPIDGTNNFVNGLPHFAV
                     SVAFVRNGRAELGVIYNPVSGECFYAERGQGAFLNGTRLPLRLVDKKLNEAIAGVEIK
                     YLRSGKLSSRMSTLAPFGTIRSMGSSTLDWCYLACGRYDVYVHGGQKLWDYAAGALIF
                     EEAGGRLTTLEGDGFWSGEHVFKRSVVAALEPKLFERWVGWIRENQ"
     gene            1489..2214
                     /locus_tag="WIC03_001940"
     CDS             1489..2214
                     /locus_tag="WIC03_001940"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEZ3884393.1"
                     /translation="MPRFYLPENLSVGQTVALPDNIVRHLNVLRVRPNENITLFDGKG
                     KAHAARLTVLEKRRAEAEILHEDTTDNESPLNITLIQSISSGDRMDFTLQKSVELGVT
                     AIQPVISERCIVRLDGERAAKRLARWQEIVISACEQSGRNTVPPVLPIIGYREALDKM
                     PSESTKLIMSINRARKLGDIRHPSGAIVFMVGPEGGWTEQEEQQAFEAGFQAVTLGKR
                     ILRTETAPLAAIAAMQTLWGDFA"
BASE COUNT          625 a          761 c          528 g          465 t
ORIGIN      
        1 cggcttcgtc gccttgtcct gatttttgtt aatccactat acatcatcgc tactaccgtt
       61 ccggcgcaac aggcattcct cgatgccgcc gaactgatgc aatggagtat agaaaccgaa
      121 gggctgggct tgaacgtcat ctcgcacaag atactcggca aagaccacgc ccaagtcgaa
      181 tttgaagcct acttccgaga cggacaacac cgatccgcgc atcacgaact gtccggcttc
      241 gtcaacatcg gcggacaatg gtattttatc gatcccaccg ttccgcatcc tgcgatgaaa
      301 caaccctgca tttgcggatc aggcaaaaaa ttcaaagcct gctgcggcaa atatctgaaa
      361 cctgtcgcat aaaaatgccg tctgaacgtt cagacggcat tttcaacgtg caaaaaaaaa
      421 ccattcatac caagggtaag tatgaatggt caatacattg cgggaaaacg tcttacttgc
      481 tgcactgccg aaaagggaga aacggcagcg gtaatcagcg gaaatgattg tacccgaatt
      541 aatgttaaga aacgttaatc gcgaaaatat attaacaaac ctgttgaaac ctattggttt
      601 tcccgtatcc acccgaccca gcgttcaaac agcttcggtt cgagcgcggc aacgaccgag
      661 cgtttgaaca cgtgttcacc actccaaaac ccgtcgcctt ccaaagtcgt cagcctgccg
      721 cccgcctcct cgaaaatcag cgcaccggcg gcataatccc acagcttctg cccgccgtgg
      781 acataaacat cataacgccc gcacgccaga taacaccagt ccaacgtact gctgcccata
      841 ctccgtatcg tcccgaaagg cgcgagcgta ctcatgcggc tggaaagttt gcccgaacgc
      901 agatatttga tttccacgcc cgcaatcgcc tcattgagtt ttttatccac gaggcgcagg
      961 ggcaggcgcg tcccgtttaa aaacgccccc tgcccccgtt cggcataaaa acattcgccg
     1021 ctgaccggat tgtagattac gcccaattcg gcgcgcccgt tgcggacaaa cgccaccgac
     1081 acggcaaaat gcggcagccc gttgacaaaa ttgtttgtcc cgtctatcgg atcgacaatc
     1141 cacagcccct tttcccccga atattgttcc cacaaagccg actgttcctg ccgcgacatt
     1201 tcctcgccca acatcgggca atcgatcaga agcggcaacg cggcggcaaa agccgtctgc
     1261 gcggcaatgt ccgcctcgct caacatcgaa ccatcttcct tgcggtgaga cggcgtattc
     1321 aaaaaacgcg gcataatttc ggtttgcgcg atatggcgca cgactttctg caaacggtgt
     1381 aacacttcct actgtcctca tattttgaac ttgcggcgcg cgaacgtata atgcccgttt
     1441 ccatcacgcc gctgcgacgg attataaccg tccgaaccgc caaaaactat gccccgattc
     1501 tacctgcctg aaaacctttc cgtcggacaa accgtcgccc tgcccgacaa catcgtccgc
     1561 cacctcaacg tcctgcgcgt ccgccccaac gaaaacatca ccctcttcga cggcaaaggc
     1621 aaggcacacg ccgcacggct gaccgttttg gaaaaacgcc gcgccgaagc cgaaatcctg
     1681 cacgaagaca caaccgacaa cgagtccccg ctcaacatca cactgataca atccatctcc
     1741 tccggcgatc gcatggattt caccctgcaa aaaagcgtcg aactcggcgt aaccgccata
     1801 cagcccgtca tcagcgaacg ctgcatcgtc cgcctcgacg gagaacgcgc cgccaaacgc
     1861 ctcgcacgct ggcaggaaat cgtcatctcc gcgtgcgaac aaagcggcag aaacaccgtt
     1921 ccccccgtac tgcccatcat cggctaccgt gaagcactcg acaaaatgcc gtctgaaagc
     1981 accaagctga ttatgagcat caaccgcgcc cgcaaactcg gcgacatccg ccacccgtcc
     2041 ggcgcaatcg tctttatggt cgggcccgaa ggcggctgga cagaacagga agaacaacag
     2101 gcatttgaag ctggctttca ggcggttaca ctcggcaaac ggattttacg cacagaaacc
     2161 gccccactcg ccgccatcgc cgcgatgcag acgctttggg gcgatttcgc ataaacagaa
     2221 atgccgtctg aaacccgttc agacggcatt ttgcagccga ttaagatagt aggttcaaat
     2281 aagatttccc ccgccgtcat tcccgcgaaa gcaagaatct agaaacgaaa aactacaggg
     2341 atttatccga aacaacaaac cctctccgcc atcattccc
//
LOCUS       DAWHSK010000264         2354 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.264, whole genome shotgun sequence.
ACCESSION   DAWHSK010000264 DAWHSK010000000
VERSION     DAWHSK010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.264"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..341)
                     /locus_tag="WIC03_001941"
     CDS             complement(<1..341)
                     /locus_tag="WIC03_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEZ3884394.1"
                     /translation="METQLYIGIMSGTSMDGADAVLIRMDGGKWLGAEGHAFTPYPDR
                     LRRQLLDLQDTGADELHRSRILSQELSRLYAQTAAELLCSQNLAPSDITALGCHGQTV
                     RHAPEHGYSIQL"
     gene            666..2240
                     /locus_tag="WIC03_001942"
     CDS             666..2240
                     /locus_tag="WIC03_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HEZ3884395.1"
                     /translation="MAQIQMSANVKTINAVFAAMLVGTVGYFIYWGLGYTHYNYAALF
                     IIATMFGVFMAFNIGGNDVANSFGTSVGAGTLTIPQALLIAAVFEVSGAVIAGGEVTN
                     TIRKGIVDLKGVDFEPIQFVFIMMSALLAAALWLLFASKKGLPVSTTHSIIGGIVGSA
                     VCMAVMNDAASGDLIRWGKLGGIGVSWVLSPVLGGAVSYFLFSRVKKNVLDYNAWAEG
                     TLKGIKQEKKAYKERHRLFFEGLSEAEKVEYATKMAHDAQIYDEPEFDPQELQSEYYR
                     GLYAFDNRKNNVDSYKALHSWIPFIASFGAMMISAMLIFKGLKNLHLGMSNVNSFLTI
                     FMIGAAVWMGTFVFAKSLKRKDLGKSTFQMFSWMQVFTACGFAFSHGANDIANAIGPF
                     AAIMDVLRTNSVAAQNVVPPIAMLTFGIALIVGLWFVGKEVIKTVGTSLAEMHPASGF
                     TAELSAASVVMGASLMGLPVSSTHILVGAVLGIGLVNRNANWKLMKPIGLAWVITLPA
                     AAVLSVVCYLVLQAVF"
BASE COUNT          491 a          575 c          664 g          624 t
ORIGIN      
        1 agctgtatgc tgtaaccgtg ttccggcgcg tgtcggacgg tttgcccgtg gcagccgagg
       61 gcggtaatgt cggatggtgc gaggttttga ctgcacagca gttcggcggc ggtttgcgca
      121 tataggcggc tgagttcttg cgacaaaatc ctgctgcggt gcagttcgtc tgcgcctgtg
      181 tcctgcaaat ccagcaattg gcggcgcagc ctgtcgggat agggggtaaa ggcgtgccct
      241 tccgcgccca gccatttgcc gccgtccatc cgtatcagta cggcatccgc cccgtccatg
      301 ctggttcccg acatgatgcc gatgtaaagc tgtgtttcca tcatcactcc caaactgatg
      361 caaaacgcca ttttaacgtg tattgacgct cgtataccga tttgctgctg cagtgtaaat
      421 aaagtgtaaa taaatgtttc aagacggatg gaaaaatatt ataatgcgcc cgcaacatcc
      481 agtagtagaa gtgtcataca aaccgtttcc ggcagcagtt ttgcattcgg tcaggtttgg
      541 gggtattcgg atgcggttca gaaggaagga tgcgtctgcc atatcccgaa acggcagttc
      601 gaccggaggc agcagtacag tgtcggcaac actcatgatt tccaccacat taaaggaaga
      661 ttgccatggc tcaaatccaa atgagcgcaa atgttaaaac catcaacgcc gtctttgccg
      721 ccatgctggt aggtacagtc ggctatttta tttattgggg cttgggttat acccattaca
      781 attacgccgc cttattcatt attgccacga tgttcggcgt gtttatggcg ttcaacatcg
      841 gcggcaacga tgttgccaat tctttcggca ccagcgtcgg tgcgggtacg ctgaccatcc
      901 cgcaggcttt gctgattgcg gcggtatttg aggtcagcgg cgcggtcatc gcgggcggtg
      961 aggtaaccaa taccatacgc aaaggcatcg tcgatttgaa gggtgttgat ttcgaaccca
     1021 tacagtttgt gtttattatg atgtccgcgc ttttggcggc ggcgttgtgg ctgttgtttg
     1081 cctcgaaaaa agggcttccg gtatctacca cccattccat tatcggcggc attgtcggca
     1141 gcgcggtatg tatggcggta atgaacgatg ccgcatcggg cgatttgata cgttggggca
     1201 agctgggcgg tattggtgtt tcttgggtat tgtcgcccgt gttgggcggc gcggtgtcct
     1261 atttcctgtt ttcgcgcgtc aagaaaaacg tcttagatta caacgcttgg gcggaaggca
     1321 cgctcaaggg catcaagcag gaaaaaaagg cctataaaga acggcaccgc ctgtttttcg
     1381 agggtttgtc cgaagccgaa aaagtcgagt acgccaccaa aatggcgcac gacgcgcaaa
     1441 tttacgacga acccgaattc gatccgcaag agctgcaatc ggagtattac cgcggtcttt
     1501 atgcgttcga caaccgtaaa aacaatgtcg attcctacaa agcgctgcat tcttggattc
     1561 cctttatcgc ttcgttcggc gcgatgatga tttccgctat gctgattttc aagggcttga
     1621 aaaacctgca tttggggatg agcaacgtca acagcttcct gaccatcttt atgataggcg
     1681 cggcggtgtg gatggggacg tttgtgtttg ccaaaagcct caagcgtaaa gacttgggca
     1741 aatcgacctt tcagatgttt tcatggatgc aggtctttac cgcctgcggc ttcgcattca
     1801 gccacggtgc gaacgatatc gccaacgcca tcggtccgtt tgccgcgatt atggatgttt
     1861 tgcgtaccaa cagcgttgcc gcgcaaaatg tcgtcccccc gattgcgatg ctgactttcg
     1921 gtatcgcgct gattgtcggt ttgtggtttg tcggtaaaga ggtgattaaa accgtcggta
     1981 cgagtttggc ggaaatgcat cctgcttcgg gttttaccgc cgaactgtcc gccgcctccg
     2041 tcgtgatggg cgcgtcgctg atggggctgc ccgtgtccag tacgcatatc ttggtcggcg
     2101 cggtactcgg catcggtctg gtcaaccgca atgccaactg gaaactgatg aagcccatcg
     2161 gtttggcgtg ggtcattacc ctgcctgccg ccgccgtatt gtcggttgtc tgctacttgg
     2221 ttttacaggc agtattctga ttgtaaaata ctgatgccgt ctgaacccgt gttcagacgg
     2281 cattttgttg atggaatgtg cgggcttgtg ccttatgcac aatctgttct gtcgggatat
     2341 gccgtttggt atag
//
LOCUS       DAWHSK010000265         2336 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.265, whole genome shotgun sequence.
ACCESSION   DAWHSK010000265 DAWHSK010000000
VERSION     DAWHSK010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.265"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(80..1489)
                     /gene="dacB"
                     /locus_tag="WIC03_001943"
     CDS             complement(80..1489)
                     /gene="dacB"
                     /locus_tag="WIC03_001943"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEZ3884396.1"
                     /translation="MNFPKTAASLLLLLASLAAHALDTGRIPQNEIAVYVQELDSGKV
                     IIDHRSDVPVNPASTMKLVTAFAAFKTFGSNYRWATEFKSNGTVNDGTLDGNLYWAGS
                     GDPVFNQENLLAVQRQLREQGIRNITGHLMLDHSLWGEVGSPDDFEADSGSPFMTPPN
                     PTMLSAGMVMVRAERNAADSTDILTDPPLSHIFAQNNLKITASQAACPSIKKLMRASF
                     SDNTLKLRGNIPESCLGKPVGVRMFALDELIRQSFTNHWLLGGGRISDGIGIADTPEG
                     AQTLAVAHAKPMKEILTDMNKRSDNLIARSVFLKLGGDGKLPAVSEQAASAVRRELAV
                     SGIDVADLVLENGSGLSRKERVTARMMAQMLETAYFSPFAQDFIDTLPIAGTDGTLRN
                     RFKQSGGLLRLKTGTLNNVRALAGYWLGDKPMAVVVIINSGRAVSLLPDLDNFVAKNI
                     ISGGDGWLDAKLMCKERRA"
     gene            1654..2220
                     /locus_tag="WIC03_001944"
     CDS             1654..2220
                     /locus_tag="WIC03_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ3884397.1"
                     /translation="MAKKISILVGSLRRASFARKVALNAAEMFPEGWQAEIVEIGHLP
                     LYNFDYDDPAVEDVPLPESYTAFRETIKASDGILFVTSENNRTIPACLKNAVDIGSKP
                     NADVAWKNKPAGIISHSVGKMGGYSSQKNLRLALSYFDMPVTGQPEVFLGNSPTLFDE
                     NGKLIDSARDFVQSYINQFVGLIERNAK"
BASE COUNT          539 a          566 c          666 g          565 t
ORIGIN      
        1 ttaaaacaat tagtaaaacc gagtttatcc tagttatcta ggacagcccc ttaaatttaa
       61 tccactatat tttcctgttt caggctcggc gttctttgca catcagtttc gcatccagcc
      121 agccgtcgcc gccggagatg atgtttttgg caacgaagtt gtccaagtcg ggcagcaggg
      181 aaacggcgcg gccgctgttg atgatgacga ccaccgccat cggtttgtcg cccagccaat
      241 aacctgcaag ggcgcggaca ttgttgagcg tgccggtttt taagcgcaac agcccgccgc
      301 tttgtttgaa gcggttgcgt aaagttccgt ctgtgccggc gatgggtagc gtgtcgatga
      361 aatcttgtgc aaacgggctg aaataagccg tttccaacat ttgcgccatc attctcgccg
      421 ttaccctttc ttttctggac aggcccgaac cgttttccaa aaccaaatcc gcaacatcga
      481 tgcccgatac ggcaagttcg cgccggacgg cagacgccgc ctgttcggaa acggcgggca
      541 gtttgccgtc gccgccgagt ttgaggaaga cggaacgcgc aattagattg tccgaacgct
      601 tgttcatgtc cgtcaaaatt tccttcatcg gtttggcgtg tgcaacggca agcgtctgcg
      661 cgccttccgg cgtgtcggct atgccgatac cgtctgaaat ccgtccgccg ccgagcagcc
      721 agtggttggt aaaactttgc cggatcagtt cgtcaagcgc gaacatccgg acaccgacag
      781 gcttgcccaa acagctctcg ggaatattgc cgcgcaattt cagcgtattg tccgaaaaag
      841 atgcacgcat cagttttttg atcgaagggc aggcagcttg ggaggcggta attttcaagt
      901 tgttttgggc gaaaatatgc gacaaaggcg gatcggtgag gatgtcggta ctgtcggcgg
      961 cattgcgttc ggcgcgcacc ataaccatac cggcagacag catagttgga ttggggggcg
     1021 tcataaacgg cgaaccgctg tcggcttcga aatcgtcggg gctgccgact tcgccccaca
     1081 ggctgtggtc gagcatcagg tgtcccgtga tattgcgtat gccttgttcg cgcaactggc
     1141 gttggacggc aagcaggttt tcctgattga aaacggggtc gccgctgccc gcccaataca
     1201 ggtttccgtc aagcgtgccg tcgtttaccg taccgttgct tttaaactcg gtcgcccagc
     1261 ggtaattgct gccgaaggtt ttgaaggcgg caaacgcggt aacgagtttc attgtggagg
     1321 cggggttgac ggggacatcc gagcggtggt caatgatgac ttttccgctg tcaagctctt
     1381 ggacatatac ggcgatttcg ttttgcggaa tgcgacccgt atcgagcgcg tgtgcggcga
     1441 gggaggcgag aagcagcagc agggaggccg ctgttttggg gaaattcata ggtaaatctg
     1501 ttacataaaa aaagcaatta taaggcaaag ccggataagt gggaacgctg tggcggcggg
     1561 ccggcttgtt tgcaggggaa atcacatata taataatcgc tatcattatg aaatgattga
     1621 gacgcacaaa cttaatattc aggaggaatg attatggcta agaaaatcag tattttggtg
     1681 ggcagcctgc gccgtgcttc ttttgcgcgc aaagtggcat tgaatgcggc ggagatgttc
     1741 cccgaaggct ggcaggcgga aatcgtcgaa atcggacatc tgccgcttta caatttcgat
     1801 tatgacgacc ctgcggtgga agatgtgccg ctgcccgaaa gctacacggc tttccgcgaa
     1861 acgattaagg cttcggacgg cattttgttc gttacgtccg aaaataaccg caccattccc
     1921 gcctgtttga aaaatgcggt ggacatcggt tctaaaccga atgccgatgt ggcttggaaa
     1981 aacaaaccgg ccggcatcat cagccattcc gtcggcaaga tggggggata cagttcgcaa
     2041 aaaaacctgc gccttgcgct gtcttatttc gatatgcccg taaccggaca gccggaagtg
     2101 tttttgggca attcgccgac actgtttgat gaaaacggca aattgattga ctcggcaagg
     2161 gattttgttc agtcatatat taatcagttt gtcggtttga ttgaaagaaa tgccaaataa
     2221 acaaaccaag tcgaaaagcc gcaaaaccgg ttcgggtttt gcggcttttt tatatgggct
     2281 gggcagcgtc aaggtttgcc gtttgccgga taatgcgcgg caaatgaccg gcgtgt
//
LOCUS       DAWHSK010000266         2327 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.266, whole genome shotgun sequence.
ACCESSION   DAWHSK010000266 DAWHSK010000000
VERSION     DAWHSK010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.266"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            42..2255
                     /locus_tag="WIC03_001945"
     CDS             42..2255
                     /locus_tag="WIC03_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEZ3884398.1"
                     /translation="MTAISPIQDTQSATLQELREWFDSYCAALPNNDKKLVLAARSLA
                     EAHYPADAATPYGEPLPDHFLGAAQMVHELDLLPDAVAATLLADIGRYVPDWNLLVSE
                     RCNSTVAELVKGVDEVQKLTHFARVDSLATPEERAQQAETMRKMLLAMVTDIRVVLIK
                     LAMRTRTLQFLSNAPDSPEKRAVAKETLDIFAPLANRLGVWQLKWQLEDLGFRHQEPE
                     KYREIALLLDEKRTERLEYIENFLNILRTELKKYNIHFEVAGRPKHIYSIYKKMVKKK
                     LSFDGLFDIRAVRILVDTVPECYTTLGIVHSLWQPIPGEFDDYIANPKGNGYKSLHTV
                     IVGPEDKGVEVQIRTFDMHQFNEFGVAAHWRYKEGGKGDSAYEQKIAWLRQLLDWREN
                     MAESGKEDLAAAFKTELFNDTIYVLTPHGKVLSLPTGATPIDFAYALHSSIGDRCRGA
                     KVEGQIVPLSTPLENGQRVEIITAKEGHPSVNWLYEGWVKSNKAIGKIRAYIRQQNAD
                     TVREEGRVQLDKQLAKLTPKPNLQELAENLGYKKPEDLYTAVGQGEISNRAIQKACGT
                     LNEPPPVPVSETTIVKQSKIKKGGKNGVLIDGEDGLMTTLAKCCKPAPPDDIVGFVTR
                     DRGISVHRKTCPSFRHLAEHAPEKVLDASWAALQEGQVFAVDIEIRAQDRSGLLRDVS
                     DALARHKLNVTAVQTQSRDLEASMRFTLEVKQVNDLPRVLASLGDVKGVLSVTRL"
BASE COUNT          597 a          759 c          563 g          408 t
ORIGIN      
        1 gttacaataa actcgttttt atcggaacgg aagaccccat catgaccgcc atcagcccga
       61 ttcaagacac gcaaagcgcg actctgcaag aattgcgcga atggttcgac agctactgcg
      121 ccgcgctgcc gaacaacgat aaaaaacttg tcttagccgc ccgttcgctg gcggaagcac
      181 attaccccgc cgatgccgcc acgccgtatg gcgagccgct gcccgaccac ttcctcggcg
      241 cggcgcaaat ggttcatgaa ctcgacctgc tccccgatgc cgtcgccgcc accctgcttg
      301 ccgacatcgg acgctacgtc cccgactgga acctattggt ttccgaacgc tgcaacagta
      361 ccgtcgccga gctggtcaaa ggtgtggacg aagtgcagaa actcacccac ttcgcccggg
      421 tggacagcct cgccacaccg gaagaacgcg cccagcaggc agaaactatg cggaaaatgc
      481 tgctggcgat ggttaccgac atccgcgtcg tgttaatcaa actggcgatg cgtacgcgca
      541 ccctgcaatt tttaagcaac gcccccgaca gccccgaaaa acgcgccgtc gccaaagaaa
      601 ccctcgacat cttcgccccg ctcgccaacc gtttgggcgt gtggcagctc aaatggcagc
      661 tcgaagattt gggcttccgc catcaagagc ccgaaaaata ccgcgaaatt gccctgcttt
      721 tggacgaaaa acgtaccgaa cgcctcgaat acatcgaaaa cttccttaat atcctgcgta
      781 cggaactcaa aaaatacaat atccactttg aagtcgccgg ccgtccgaaa cacatctact
      841 ccatttacaa aaaaatggtg aagaaaaaac tcagcttcga cgggttgttc gacatccgcg
      901 ccgtgcggat tctggttgat accgtccccg agtgttacac cacactgggc attgtccaca
      961 gcctctggca gcccattccc ggcgagttcg acgactacat cgccaacccg aaaggcaacg
     1021 gctataaaag tttgcacacc gtcatcgtcg gcccggaaga caaaggcgtg gaagtgcaaa
     1081 tccgcacctt cgatatgcac caattcaacg aattcggtgt cgccgcgcac tggcgttaca
     1141 aagagggcgg caaaggcgat tccgcctacg aacaaaaaat cgcctggtta cgccaacttt
     1201 tggactggcg cgaaaacatg gcggaaagcg gcaaggaaga cctcgccgcc gccttcaaaa
     1261 ccgagctttt caacgacacg atttatgttt tgaccccgca cggcaaagtc ctctccctgc
     1321 ccacaggcgc gacccccatc gacttcgcct acgccctgca cagcagcatc ggcgaccgtt
     1381 gccgcggtgc gaaagtcgaa gggcagattg tgccgctgtc caccccgctc gaaaacggac
     1441 agcgtgtcga aatcattacc gccaaagaag ggcatccttc cgtcaactgg ctttacgaag
     1501 gctgggtcaa atccaacaag gcaatcggca aaatccgcgc ctacatccgc cagcaaaacg
     1561 ccgacaccgt gcgcgaagaa ggccgcgtcc aactcgacaa acagcttgcc aaactcacgc
     1621 ccaaacccaa cctgcaagag cttgccgaaa atctcggcta caaaaagcca gaagacctct
     1681 acaccgccgt cggacaaggc gaaatttcca accgcgccat ccaaaaagcc tgcggcacgc
     1741 tgaacgaacc gccgcccgta cccgtcagcg aaaccaccat cgtcaaacag tccaaaatca
     1801 aaaaaggcgg caaaaacggc gtgctcatcg acggcgaaga cggtctgatg accacgcttg
     1861 ccaaatgctg caaacccgcg ccgcccgacg acattgtcgg cttcgttacc cgcgatcgcg
     1921 gcatttcggt acaccgcaaa acctgcccct ctttccgaca cctcgccgaa cacgcgcccg
     1981 aaaaagtact ggacgcaagt tgggcggcgt tgcaggaagg acaagtgttt gccgtcgata
     2041 tcgaaatccg cgcccaagac cgctccgggc ttttgcgcga cgtatccgac gcgctcgccc
     2101 gccacaaact caacgttacc gccgtgcaaa cccagtcccg cgacttggaa gccagtatgc
     2161 ggttcacgct cgaagtcaaa caggtcaacg acctcccgcg cgtcctcgcc agcctcggcg
     2221 acgtcaaagg cgtattgagc gttacccggc tttaaataca aaaatgccgt ctgaaagccg
     2281 aataacgctt cagacggcat tttgattgcc ggggtttgct atttttt
//
LOCUS       DAWHSK010000267         2289 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.267, whole genome shotgun sequence.
ACCESSION   DAWHSK010000267 DAWHSK010000000
VERSION     DAWHSK010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.267"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            150..644
                     /locus_tag="WIC03_001946"
     CDS             150..644
                     /locus_tag="WIC03_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884399.1"
                     /translation="MENRFEKIVKDIFSFTQFVSEAENEIPNQSSRYYDCWWELEILN
                     ASALEDWEISGKPEKWEKWTEKYQTEALQSVKHLFAEEVPENNIVCNISHFLHFADRA
                     EADIPNQSRSYAEYWWEMEMFKMSVLEDWKRLGKPLNWEKWQRQYQSEAFELVKKLNR
                     SKQE"
     gene            764..1897
                     /locus_tag="WIC03_001947"
     CDS             764..1897
                     /locus_tag="WIC03_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884400.1"
                     /translation="MFKKFKPVLLSFFALVFAFWLGTGIAYEINPRWFLSDTATEVPE
                     NPNAFVAKLARLFRNADRAVVIVKESMRTEESLAGAVDDGPLQSEKDYLALAIRLSRL
                     KEKAKWFHVTEQEHGEEVWLDYHIGEGGLVAVSLSQRSPEAFVNAEYLYRNDRPFSVN
                     VYGGTVHGENYETTGEYRVVWQPDGSVFDASGRGKIGEDVYEHCLGCYQMAQVYLAKY
                     RDVANNEQKVWDFREESNRIASDSRDSVFYQNMRELMPRGMKANSLVVGYDADGLPQK
                     VYWSFDNGKKRQSFEYYLKNGNLFIAQSSTVALKADGVTADIQTYHAQQTWYLDGGRI
                     IREEKQGDKLPDFPLNLENLEKEVSRYAEAAARRSGGRRDLSH"
     gene            complement(1984..2259)
                     /locus_tag="WIC03_001948"
     CDS             complement(1984..2259)
                     /locus_tag="WIC03_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884401.1"
                     /translation="MNEGEVVLTPEQIQTLRGYASRGDTYGGWRYLANLGDRYADDAA
                     AIVGKDTNLNGLNLWMKKGVENLWDDTVGKKIRLMCISVFWIVVFNL"
BASE COUNT          671 a          444 c          574 g          600 t
ORIGIN      
        1 gctctgatta aaaacccaac aatcaaaatg ccgtctgaag cggtattcgg ctttcagacg
       61 gcattttctc cgattagcac aggatgccgg ggcttgccgg cttgattttg ccgaaattgt
      121 tggcacgcaa ttgggagggg cggcaaccta tggaaaatag atttgaaaaa atcgttaagg
      181 atatttttag ttttacccaa tttgtttccg aagcagaaaa tgagattcct aaccaatcct
      241 cccgttatta cgactgttgg tgggaattgg aaatcttgaa tgcttccgca ttggaagatt
      301 gggaaatatc gggaaagcct gaaaaatggg agaaatggac agaaaaatat cagaccgaag
      361 cgttacagtc agtcaagcat ctatttgccg aagaagttcc tgaaaataat atagtttgca
      421 atatttctca ttttttgcat tttgccgatc gggcagaagc cgacattccc aatcaatcac
      481 gttcatatgc cgaatattgg tgggaaatgg aaatgttcaa aatgtcggtt ttagaagatt
      541 ggaaacgctt gggaaaaccg ttaaattggg aaaaatggca aagacaatat caatccgaag
      601 catttgaatt agtaaaaaaa ttgaatcgaa gcaaacaaga gtaacaagcc gcagcctgca
      661 tagggatttt taaagtacgg cagcaaccgt agattaaatt aaaaatcaaa caatcaaagc
      721 tcgtctgata taatcgatat attctgttaa acgaaaaata aatatgttta aaaaattcaa
      781 accggtactg ttgtcatttt ttgcacttgt atttgccttt tggctgggaa caggtattgc
      841 ctatgagatt aatccgcgtt ggtttttgag cgatacggca actgaagtac ctgaaaatcc
      901 gaatgctttt gtggcgaaac ttgcccgcct gttccgaaat gccgacaggg cggttgtcat
      961 cgtgaaggaa tcgatgagga ctgaggaaag tcttgccgga gctgtggatg acggtccgtt
     1021 gcagtcggag aaggattatc tcgcgctcgc tatccggctc agtcgtttga aagaaaaggc
     1081 gaaatggttt cacgtaacgg agcaggaaca tggggaagag gtttggctgg attaccatat
     1141 cggcgagggc ggtttggttg cggtttcgct ttcgcaacgc tcgccggaag cgtttgttaa
     1201 tgccgaatat ctgtatcgga acgatcgtcc gttttctgta aatgtgtacg gcggaacggt
     1261 tcacggagaa aattatgaaa cgacaggaga atatcgggtt gtttggcaac cggacggttc
     1321 ggtatttgat gcgtcggggc gtgggaaaat cggggaagat gtttatgagc attgcctcgg
     1381 gtgttatcag atggcccagg tatatttggc gaaataccgg gatgtcgcga ataacgagca
     1441 gaaggtttgg gacttccgcg aagagagcaa ccggattgcg tcggactcgc gcgattctgt
     1501 gttttatcag aatatgcggg aattgatgcc acgggggatg aaggcgaaca gtcttgtggt
     1561 cggctatgat gcggacggtc tgccgcaaaa agtctattgg agtttcgaca atgggaaaaa
     1621 acgccagagt ttcgaatatt atttgaaaaa cggaaatctt tttattgcac aatcttcgac
     1681 ggtagcattg aaagcggatg gcgtaacggc ggatatacag acctatcatg cgcaacagac
     1741 gtggtatttg gatggcgggc ggattatccg cgaagagaaa cagggagaca aactgcctga
     1801 ttttcctttg aacttggaaa atttggaaaa agaggtgagc cgttatgcag aggctgcggc
     1861 gagacgttcg ggcggcaggc gcgacctttc tcactgaacg caaatgccgt ctgaaaccgg
     1921 ttttcagacg gcattggaga gtcaaacgct ttttgcaatg ccgtatatgc cgaatccgat
     1981 tcgctacaaa ttgaaaacca caatccaaaa aacggaaata cacattaaac ggatcttttt
     2041 accgaccgta tcatcccata ggttttccac accttttttc atccataaat tcaaaccatt
     2101 taagtttgta tccttaccga caattgcagc agcatcatcc gcataacggt cacccaaatt
     2161 agccaaataa cgccaaccgc cataggtatc gccacgggaa gcataaccac gcaaggtttg
     2221 gatttgttct ggtgttaaaa caacttcacc ctcattcata ataagatcct ttaaattatt
     2281 ttttacttg
//
LOCUS       DAWHSK010000268         2285 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.268, whole genome shotgun sequence.
ACCESSION   DAWHSK010000268 DAWHSK010000000
VERSION     DAWHSK010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.268"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            297..809
                     /locus_tag="WIC03_001949"
     CDS             297..809
                     /locus_tag="WIC03_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEZ3884402.1"
                     /translation="MKNFNGKLILIGLMGAGKTTLGRQMAQRLDYRFYDSDHEIAAAA
                     GVPIPTIFEMEGEQGFRSRETAILKKLVILPHIVLSTGGGAVLKEENRALIRKSGTVV
                     YLHAPPETLLERTRCDNSRPLLQVADPLAKLRELYAARDPVYRQTADFTVESANCRET
                     VQTLLKRLSR"
     gene            889..1968
                     /gene="aroB"
                     /locus_tag="WIC03_001950"
     CDS             889..1968
                     /gene="aroB"
                     /locus_tag="WIC03_001950"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEZ3884403.1"
                     /translation="MKTLTVHTPSHSYPIFIGNGLLPQAGSLLKPHLGKRAAIIANET
                     VAPLYLGTLQTALDAAGVSHFSIILPDGEAHKNWQTLNLIFDGLMQNRAERKTTLIAL
                     GGGVIGDMVGFAAATYQRGAPFVQIPTTLLSQVDSSVGGKTAINHPLGKNMIGAFYQP
                     QAVLADLDTLHTLPVRELSAGMAEVIKYGALGDIGFFEWLEQNISGLMTLDREKLAQA
                     VYRCCQMKADIVAQDETEQGIRAWLNLGHTFGHAIETEMGYGTWLHGEAIAAGCVLAA
                     RLSEQLGKTSAADTARLAALLEAAGLPSAPPVFAFEKWLEHMSHDKKVSGGIMRFIGL
                     NRLGEANITEITDTDILRRTLQPYL"
BASE COUNT          563 a          709 c          547 g          466 t
ORIGIN      
        1 tattgataaa catattatct tcaatatatt caattggata attgtttacc taagcaaaga
       61 taattgcctt ttcctgacaa ataagtgaaa tcaacggatt gtcaaaacac agcctgaaat
      121 aaaaaacctc cctgatttct tttatttttc cttaaaatca gaaaggttcg ggatggtcgg
      181 gttatttttc caaacgtacc gccgccctgc cgatttcgta taaaattccg ccgtaacccg
      241 acaagcccga accctgtcgc cccgaaaggc ggggtgtcaa acattaagga attgtgatga
      301 aaaactttaa cggcaaactc atcctcatcg gactgatggg cgcgggcaaa accacgctgg
      361 gccggcaaat ggcgcagcgg ctggattacc gtttttacga cagcgatcac gaaatcgccg
      421 cagccgccgg cgttcccatc cccaccatat ttgaaatgga aggcgaacag ggattccgtt
      481 cgcgcgaaac cgccatactc aaaaagctgg ttatcctgcc ccatatcgtc ctgtccaccg
      541 gcggcggcgc ggtgttaaaa gaagaaaacc gcgcccttat ccgcaaaagc ggcacggtcg
      601 tctatctgca cgccccgccc gaaaccctgc tcgaacgcac gcgctgcgac aacagccgtc
      661 ctttgctgca agttgccgat cctttggcga aattacgtga actctacgcc gcacgcgacc
      721 ccgtttaccg ccaaaccgcc gactttaccg tagaatcggc aaactgccgg gaaaccgtgc
      781 aaaccctgct caaacgctta tcccgataaa ccggcatatg cgccgcgccc agaaaaccaa
      841 gccgcgcccc gcccggcggg ccggcggttc aaactttaag gaacaacaat gaaaacactg
      901 accgtacaca ccccgtccca cagctacccc atctttatcg gcaacggact gctgccgcag
      961 gcaggaagcc tgctcaaacc gcatttgggc aaacgcgccg ccatcatcgc caacgaaacc
     1021 gtcgccccgc tctacctcgg cacgcttcag acggcattgg atgcggcagg cgtatcccat
     1081 ttcagcatca tcctgcccga cggcgaggcg cacaaaaact ggcagacgct caacctcatc
     1141 tttgacgggc tgatgcaaaa ccgcgccgaa cgcaaaacca cattaatcgc actgggcggc
     1201 ggcgtgatcg gcgacatggt cggctttgcc gctgccacct accagcgcgg cgcaccgttc
     1261 gttcaaatac cgaccacact gttgagtcaa gtcgactcat cggtgggcgg caaaaccgcc
     1321 atcaaccacc cgctcggcaa aaatatgatc ggcgcattct accagccgca ggcggtgctt
     1381 gccgacttgg acacgctgca caccctgccc gtccgcgaat tgtccgccgg tatggcggaa
     1441 gtcatcaaat acggcgcgct cggcgacatc ggttttttcg aatggctcga acaaaacata
     1501 tccggactga tgacgctcga tcgggaaaaa ctcgcccaag ccgtgtaccg ctgctgccaa
     1561 atgaaggcag acatcgtcgc ccaagacgaa accgaacagg gcatacgcgc atggctcaac
     1621 ctcggacaca ccttcggaca cgccattgaa accgagatgg gttacggcac ttggctgcat
     1681 ggagaagcca tcgccgccgg ctgcgtgttg gcggcgcgtt tgtccgaaca actgggcaaa
     1741 acctccgccg cagataccgc gcggctcgcc gccctgctcg aagccgctgg actgccgtcc
     1801 gcaccgcccg tgtttgcctt tgaaaaatgg ctcgaacata tgagccacga taaaaaagtc
     1861 agcggcggca tcatgcgctt tatcggtctg aaccggctgg gcgaagccaa catcaccgaa
     1921 attaccgaca cggacatcct ccgccgcacc ctgcaaccgt atctctgatt tcctctgccg
     1981 atgtgctgcc gcgcgggttt gacgcacgat gatgattttc catcatcttt ctccgtaaaa
     2041 gcgggaatcc ggtccgttcg gtttcggtcg tttccgataa gttcccgttg cttttcattt
     2101 ctagattccc actttcgcag gaatgacggt ggaaaggttt ttgttgtttc ggagaagttt
     2161 ctgcaaccct agaatctcgt tatttccaca aaaaacagaa acccaaaaca gcaacttaaa
     2221 acctcgtcat tcccgcaaaa gcgggaatcc ggtccgttcg gtttcggtcg tttccgataa
     2281 attcc
//
LOCUS       DAWHSK010000269         2263 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.269, whole genome shotgun sequence.
ACCESSION   DAWHSK010000269 DAWHSK010000000
VERSION     DAWHSK010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.269"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..330
                     /locus_tag="WIC03_001951"
     CDS             <1..330
                     /locus_tag="WIC03_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEZ3884404.1"
                     /translation="LMVSVTEPGGTGTAGAVDGFDVGAKTGTARKFVNGRYADNKHIA
                     TFIGFAPAKNPRVIVAVTIDEPTAHGYYGGVVAGPPFKKIMGGSLNILGISPTKPLTA
                     AAVKTPS"
     gene            355..1833
                     /locus_tag="WIC03_001952"
     CDS             355..1833
                     /locus_tag="WIC03_001952"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEZ3884405.1"
                     /translation="MFSKLTPLAETGIPTLSCANAAGRLLHSDSRQIKQGDIFVACPG
                     EYADGRSYIPAAVANGAAFVFWDDDGKFAWNPEWKVPNQGIKDLKHRAGILAAQVYGN
                     VSDGLKVWGVTGTNGKTSITQWLAQAADLLGEKTAIVGTVGNGFWGALEETTHTTPAP
                     VDVQTLLYRFRQQGATVAAMEVSSHGLDQSRVNGVSFRSAIFTNLTRDHLDYHGTMEA
                     YGAIKSRLFYWHGLKHAVINVDDEYGAELAGRLKKDCPDLAVYSYGFSEHADIRITDF
                     TASSDGIAAVFQTLWGEGKCRTRLLGRFNAQNLAACIALLCANGYPLDNVLDVLAKIR
                     PASGRMDCIMNSGKPLVVVDYAHTPDALEKALATLQEIKPQGAALWCVFGCGGNRDRG
                     KRPLMGAAAVQGADKVVVTSDNPRLENPHDIINDILPAVPAPECVEADRAAAIRYAVE
                     QAAANDIILIAGKGHENYQDVQGVKHRFSDLEIVGQALLTRK"
     gene            1856..>2263
                     /locus_tag="WIC03_001953"
     CDS             1856..>2263
                     /locus_tag="WIC03_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884406.1"
                     /translation="MKQSARIKNMDQTLKNTLGICALLAFCFGAAIASGYHLEYEYGY
                     RYSAVGALASVVFLLLLARGFPRVSSVVLLIYVGTTALYLPVGWLYGAPSYQIVGSIL
                     ESNPAEAREFVGNLPGSLYFVQALFFIFGLTVWK"
BASE COUNT          508 a          641 c          599 g          515 t
ORIGIN      
        1 ctgatggttt ccgtgaccga gccgggcggc accggtacgg cgggtgcggt ggacggtttc
       61 gatgtcggcg cgaaaaccgg cacggcgcgc aagttcgtca acgggcgtta tgccgacaac
      121 aaacacatcg ctacctttat cggttttgcc cccgccaaaa acccccgtgt gattgtggcg
      181 gtaaccattg acgaaccgac tgcccacggt tattacggcg gcgtagtggc agggccgccc
      241 ttcaaaaaaa ttatgggcgg cagcctgaac atcttgggca tttccccgac caagccactg
      301 accgccgcag ccgtcaaaac accgtcttaa tccgagtatc aacgagattg ttttatgttc
      361 agcaagttaa cccctttggc tgaaaccggc atcccgaccc tgtcgtgtgc aaacgcggca
      421 gggcgtttgt tgcattcaga cagccgccaa atcaaacaag gcgatatttt cgttgcctgt
      481 ccgggcgaat atgccgacgg acgcagttat atccccgccg ccgttgccaa cggcgcggct
      541 tttgtttttt gggacgacga tggcaaattc gcgtggaatc ccgaatggaa agtccccaat
      601 caaggcatca aagatttgaa acaccgtgcc ggcatattgg cggcgcaagt ttacggcaac
      661 gtttcagacg gtctcaaagt ctggggcgta accggaacca acggcaaaac ctccatcacg
      721 caatggctgg cgcaagctgc cgatttgttg ggcgaaaaaa ccgccattgt cggcacggtc
      781 ggcaacggct tttggggtgc attggaagaa accacgcata ccacacccgc ccccgtcgat
      841 gtccaaaccc tgctctaccg tttccgtcaa caaggcgcaa cagtcgccgc gatggaagtc
      901 tccagccacg ggcttgacca gtcgcgcgtc aacggcgtgt cattccgcag cgcaatcttt
      961 accaacctca cccgcgacca cctcgactac cacggcacga tggaagccta cggtgccatc
     1021 aagtcgcgcc tgttttactg gcacggcttg aaacacgccg ttatcaatgt agatgacgaa
     1081 tacggcgcgg aactcgcagg tcgtctgaaa aaagattgtc ccgatttggc cgtttatagc
     1141 tacggcttca gcgaacacgc cgacatccgc attaccgact ttaccgcctc ttcagacggc
     1201 atagcagccg tattccaaac cctgtggggc gaagggaaat gccgcacgcg cctgctcgga
     1261 cggttcaacg cgcaaaacct cgccgcctgc atcgccttgc tgtgcgccaa cggctatccg
     1321 cttgataatg tattggatgt gctggcaaaa atccgtcccg cttcagggcg catggactgc
     1381 atcatgaaca gcggcaaacc tttggtcgtt gtcgattatg cccatacgcc cgacgcattg
     1441 gaaaaagcac tcgccacctt gcaggaaatc aaaccgcagg gtgcggcttt gtggtgcgta
     1501 ttcggttgcg gcggcaaccg cgatcgcggc aaacgcccgc tgatgggcgc ggcagccgta
     1561 cagggcgcgg ataaagtcgt cgtcaccagc gacaatccgc gtttggaaaa tccgcacgac
     1621 atcatcaacg acatcctgcc tgccgttccc gcgcccgaat gcgtcgaagc cgaccgtgcc
     1681 gccgccatcc gttatgcggt tgaacaagcc gccgcaaacg acatcatcct gattgccggc
     1741 aaagggcatg aaaactatca ggatgtacaa ggcgtgaagc accgtttttc cgatcttgaa
     1801 atcgtcggac aggctttgtt aactcgtaaa taatgggata ttcggacggc atcgtatgaa
     1861 acaatccgcc cgaataaaaa atatggatca gacattaaaa aatacattgg gcatttgcgc
     1921 gcttttagcc ttttgttttg gcgcggccat cgcatcaggt tatcacttgg aatatgaata
     1981 cggctaccgt tattctgccg tgggtgcttt ggcttcggtt gtatttttat tattattggc
     2041 acgcggtttc ccgcgcgttt cttcagttgt tttactgatt tacgtcggca caaccgccct
     2101 atatttgccg gtcggctggt tgtatggtgc gccgtcttat cagatagtcg gttcgatatt
     2161 ggaaagcaat cctgccgagg cgcgtgaatt tgtcggcaat cttcccgggt cgctttattt
     2221 tgtgcaggca ttatttttca tttttggctt gacagtttgg aaa
//
LOCUS       DAWHSK010000270         2233 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.270, whole genome shotgun sequence.
ACCESSION   DAWHSK010000270 DAWHSK010000000
VERSION     DAWHSK010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.270"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..750)
                     /locus_tag="WIC03_001954"
     CDS             complement(<1..750)
                     /locus_tag="WIC03_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEZ3884407.1"
                     /translation="MKFARLLFLCAALAGTQAAAAEMVQIEGGSYRPLYLKKDTGLIK
                     VKPFKLDKYPVTNAEFAEFVNSHPKWQKGKVSSKQAEPAYLKHWMKNGSRSYAPKAGE
                     LKQPVTNVSWFAANAYCAAQGKRLPTIDEWEFAGLASATQKNGSNEPGYNRTILDWYA
                     DGGRKGLHDVGKGRPNYWGVYDMHGLIWEWTEDFNSSLLSSGNADAQMFCSGASVGSS
                     DSSNYAAFLRYGIRTSLQSKYVLHNLGFRCTS"
     gene            complement(930..2090)
                     /gene="nirK"
                     /locus_tag="WIC03_001955"
     CDS             complement(930..2090)
                     /gene="nirK"
                     /locus_tag="WIC03_001955"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase, copper-containing"
                     /protein_id="HEZ3884408.1"
                     /translation="MKRQTLAALIASVFALAACGEQAAKPAETPAATASAEAPAASNS
                     QAAAETPSSELPVIDAIVTHAPEVPPPTDRDHPAKVRVKMETVEKTMKMDDGVEYHYW
                     TFDGDVPGRMIRVREGDTVEVEFSNNPSSTVPHNVDFHAATGQGGGAAATFTAPGRTS
                     TFSFKALQAGLYIYHCAVAPVGMHIANGMYGLILVEPKEGLPKVDKEFYIVQGDFYTK
                     GKKGAQGLQPFDMDKAIAEQPEYVVFNGHVGSIAGDNALKAKAGETIRMYVGNGGPNL
                     VSSFHVIGEIFDKVYVEGGKLINENVQSTIVPAGGSAIVEFKVDIPGSYTLVDHSIFR
                     AFNKGALGQLKVEGEENPEIMTQKLSDTAYAGNGAASAASAPAASASEKSVY"
BASE COUNT          538 a          509 c          594 g          592 t
ORIGIN      
        1 gcttgtgcaa cggaagccca agttgtgcaa gacgtatttg gattgtaggc tggtgcggat
       61 gccgtagcgg aggaatgcgg catagttgga cgagtcgctc gacccgacag atgcgccgct
      121 gcaaaacatt tgcgcatcgg cattgccgga agaaagcagg ctgctgttga aatcttccgt
      181 ccattcccaa atcagaccgt gcatatcgta aacgccccag tagttcgggc ggcctttgcc
      241 gacatcgtgc aggcctttac gaccgccatc cgcataccaa tcgagaatag tgcggttgta
      301 gccgggttcg tttgagccgt ttttctgcgt ggcggaagca agtccggcaa attcccattc
      361 gtcaatggtc ggcaggcgtt tgccttgtgc agcgcaatag gcgttggcgg caaaccagga
      421 aacattggtt accggttgtt ttaattcgcc cgccttcggc gcatagctgc ggctgccgtt
      481 tttcatccaa tgcttcaggt aagcaggctc tgcctgtttg gaactgacct tgcccttttg
      541 ccatttgggg tgactgttga caaattcggc aaactcggca ttggtaacgg gatatttatc
      601 cagtttgaac ggtttgactt taatcaggcc ggtatctttt ttcagataaa gcgggcggta
      661 gctgccgcct tcgatttgaa ccatttcggc agccgccgct tgggtgccgg cgagagccgc
      721 acagagaaag agtaaccggg caaacttcat attatgcctc ctgacaggcg gttaacattg
      781 atttttcaga cggcatttta aacgagaacc gcgaatgccg tctgaagaat caagcttcca
      841 aagtaaaaag gttgatgatt aaaaaaccac tgccgtgcgt aatgaagtac ggcggcagtg
      901 gtttgttccg ctaatgacgg atatccgatt tagtaaacgc ttttttcgga tgcagaggct
      961 gccggagcgg aagctgcgga agccgcaccg ttgccggcgt aagcggtatc actcagtttt
     1021 tgagtcataa tttcagggtt ttcctcaccc tctactttca attgacccaa cgcgcctttg
     1081 ttgaatgcgc ggaagataga gtggtcgacc aaggtgtagc tgcctggaat gtcgactttg
     1141 aattcgacaa tggcggaacc gccggcagga acaattgtac tttgtacgtt ttcgttaatc
     1201 agtttaccac cttcaacata tactttgtcg aagatttcgc cgataacgtg gaaagaagat
     1261 accaagttag gaccaccgtt accaacgtac atacgaatag tttcaccagc tttagctttc
     1321 agagcgttat cgccggcgat agagcctacg tgaccgttga atacaacgta ttcaggttgt
     1381 tcagcaatcg ctttatccat atcgaaaggt tgcaggcctt gagcaccttt tttaccttta
     1441 gtatagaagt caccttgtac gatgtagaac tctttgtcca ctttaggcag accttcttta
     1501 ggttcaacca agatcaaacc gtacataccg ttggcgatgt gcatacctac aggagcaacg
     1561 gcacagtggt agatgtacaa accagcttgc agcgctttga agctgaatgt ggaagtacgg
     1621 cccggagcgg taaaggttgc agccgcgccg ccgccctgac cggtagccgc gtggaagtcg
     1681 acgttatggg gaacggtaga agaaggattg ttggagaatt ccacttcaac cgtatcgcct
     1741 tcgcgtacgc ggatcatgcg gcccggaaca tcgccgtcaa atgtccagta atggtattcc
     1801 acgccgtcgt ccattttcat ggttttttca acggtttcca ttttaacgcg tactttagcc
     1861 ggatggtcgc gatcagttgg aggtggaact tccggagcgt gagttacgat agcatcgatt
     1921 acaggcagtt ctgaagaagg agtttctgca gcagcttgtg agttggaagc ggcaggagct
     1981 tcagcagaag cggtagcagc tggagtttca gcaggtttag cagcttgctc gccgcatgcg
     2041 gccagtgcaa atacagaagc gataagtgcg gctaaggttt ggcgtttcat gtttaaagtt
     2101 tccttatagt aagaaaagtt aagaatcctg ataggatggt ttgaaatttt gtatgattat
     2161 gtaggcgctt gtttggtcat attttgatgt aaatcaaatt attcagggaa tatgaatctt
     2221 ttactaccta aat
//
LOCUS       DAWHSK010000271         2221 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.271, whole genome shotgun sequence.
ACCESSION   DAWHSK010000271 DAWHSK010000000
VERSION     DAWHSK010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.271"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            104..1915
                     /gene="typA"
                     /locus_tag="WIC03_001956"
     CDS             104..1915
                     /gene="typA"
                     /locus_tag="WIC03_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEZ3884409.1"
                     /translation="MKQIRNIAIIAHVDHGKTTLVDQLLRQSGTFRANQQVDERVMDS
                     NDLEKERGITILAKNTAIDYEGYHINIVDTPGHADFGGEVERVLGMVDCVVLLVDAQE
                     GPMPQTRFVTKKALALGLKPIVVINKIDKPSARPSWVIDQTFELFDNLGATDEQLDFP
                     IVYASGLSGFAKLEETDESNDMRPLFDTILKYTPAPSGSADETLQLQISQLDYDNYTG
                     RLGIGRILNGRIKPGQTVAVMNHDQQIAQGRINQLLGFKGLERVPLEEAEAGDIVIIS
                     GIEDIGIGVTITDKDNPKGLPMLSVDEPTLTMDFMVNTSPLAGTEGKFVTSRQIRDRL
                     QKELLTNVALRVEDTADADVFRVSGRGELHLTILLENMRREGYELAVGKPRVVYRDID
                     GQKCEPYENLTVDVPDDNQGAVMEELGRRRGELTNMESDGNGRTRLEYHIPARGLIGF
                     QGEFMTLTRGVGLMSHVFDDYAPVKPDMPGRHNGVLVSQEQGEAVAYALWNLEDRGRM
                     FVSPNDKIYEGMIIGIHSRDNDLVVNPLKGKKLTNIRASGTDEAVRLTTPIKLTLEGA
                     VEFIDDDELVEITPQSIRLRKRYLSELERRRHFKKLD"
BASE COUNT          568 a          627 c          584 g          442 t
ORIGIN      
        1 gaaaggcaag ggcttcagac ggcatcttca tttcccaaat actgtccggt aaagcgtggt
       61 ataatgcgca gttatctctt acagtttttg aaaaacatta attatgaaac aaatccgcaa
      121 catcgccatc atcgcccacg tcgaccacgg caaaaccaca ttggtcgacc aactgctgcg
      181 ccaatccggc acattccgag ccaaccagca ggttgacgag cgcgtgatgg acagcaacga
      241 cctcgaaaaa gaacgcggca tcaccatcct cgccaaaaac accgccatcg attacgaagg
      301 ctaccacatc aatatcgtcg atacgccggg acacgccgac ttcggcggcg aagtagagcg
      361 cgttttgggg atggtggact gcgtcgtctt gttggtggac gcgcaggaag gcccgatgcc
      421 gcaaacccgt ttcgtgacca aaaaagcctt ggctttgggg ctgaaaccga ttgtcgtcat
      481 caacaaaatc gacaagccgt ccgctcgtcc gagctgggtc atcgaccaaa ctttcgagct
      541 gttcgacaac ttgggcgcga ccgacgagca gttggatttc ccgattgttt acgcttcagg
      601 gttgagcggt ttcgccaaat tggaagaaac cgacgagagc aacgacatgc gtccgctgtt
      661 cgatactatc ttaaaatata cgcctgcacc gagcggcagc gcggacgaaa cgctgcaact
      721 gcaaatttcc caactcgact acgacaacta caccggccgc ctcggtatcg gtcgtatctt
      781 gaacggacgc atcaaacccg gccaaaccgt tgccgtcatg aaccacgatc agcaaatcgc
      841 ccaaggccgc atcaaccagc ttttgggttt caaaggtttg gaacgcgtgc cgcttgaaga
      901 agccgaagcc ggcgacatcg tgattatttc cggtatcgaa gacatcggca tcggcgtaac
      961 catcactgat aaagataacc ccaaaggcct gccgatgttg agcgtggacg aaccgacgct
     1021 gaccatggac tttatggtta acaccagccc gttggcgggc acggaaggca aattcgtaac
     1081 cagccgccaa atccgcgacc gcctgcaaaa agaattgctg accaacgtcg ccctgcgcgt
     1141 ggaagatacc gccgatgccg acgtgttccg cgtatccggg cgcggcgagc tgcacctgac
     1201 cattttgctg gaaaacatgc gccgcgaagg ctacgaactc gccgtcggca aaccgcgcgt
     1261 cgtgtaccgc gacatcgacg gtcaaaaatg cgaaccgtat gaaaacctga ccgtggacgt
     1321 acccgacgac aaccaaggcg cggtaatgga agaactcggc cgccgccgtg gcgaactgac
     1381 taatatggaa agcgacggca acggacgcac ccgcctcgaa taccatattc cagcgcgcgg
     1441 cttgatcggc ttccaaggcg aatttatgac cctgacgcgc ggggtcgggc tgatgagcca
     1501 cgtgttcgac gattacgcgc ccgtcaaacc cgatatgcct ggccgccaca acggcgtgct
     1561 ggtgtcccaa gagcagggcg aggcagtcgc ttacgccttg tggaatctgg aagaccgcgg
     1621 ccgtatgttc gtatcgccca acgacaaaat ctacgaaggt atgattatcg gcatccacag
     1681 tcgcgacaac gatttggtgg tcaacccgct caaaggcaaa aaacttacca acatccgtgc
     1741 cagcggtacc gacgaagccg ttcgcctgac cacgccgatt aagctgacgc tggaaggtgc
     1801 ggtcgagttt atcgacgatg atgagctggt agaaatcacg ccgcaatcca tccgtctgcg
     1861 caagcgttac ttgagcgaat tggaacgccg ccgccatttc aaaaagctag attgatgttt
     1921 actggattaa tgtttaaatg atacgcgatg ccgtctgaaa aatttcagac ggcatttttt
     1981 attcggacgg gctttgcggc ttcttgaagc tgtttcagac ggcgtttttc ctacccaatc
     2041 aagaaactgc cgccattttt ccagcggcat atcgccccgt cgcgtttcgg tatcgggttc
     2101 ggcttcccgg cagcatgtcg aacaatgccg tttgaaggaa tacgcctttt gagtccttac
     2161 aagcttagga agaggatata gtggattaac aaaaatcagg acaaggcgac gaagccgcag
     2221 a
//
LOCUS       DAWHSK010000272         2216 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.272, whole genome shotgun sequence.
ACCESSION   DAWHSK010000272 DAWHSK010000000
VERSION     DAWHSK010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.272"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            136..2016
                     /locus_tag="WIC03_001957"
     CDS             136..2016
                     /locus_tag="WIC03_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HEZ3884410.1"
                     /translation="MNIFYEESGQFKIAAIIQKNDATYQVDTPHGKRTKVKANNVFTE
                     FDGDMAAFLENAQAQAADIDTDLLWEVCGEEEFTAEAIAEEYYGHAPTKTELAATLIA
                     LYAAPMYFYKKAKGVFKAAPEETLKQALAAIERKKQQDAQIDAWAEALKRGEMPSEIA
                     ADLKTILHAPDKQSLTYKAFTKAADALKTSAYELAKKTGGITSIPQYLQDGFEIKYFP
                     KGTGFPDLPLPEMPDLPKADVTAFSIDDESTTEVDDALSLTDLDNGTKRVGIHIAAPS
                     LAIKQGDKMKKNIMERLSTVYFPGGKITMLPENWIAAFSLDAGAHRPAVSIYFDVDGE
                     FNVGAPTCKIEAVNISENLRIQAIEPHFNAEAGLDEAGEMMFAHHQDLIWFYQFAITL
                     QKARGKYEPNRAPQYDYSIELDKEDNVSVVRRERGSPIDTLVSEMMILANSTWAQMLH
                     DNDLPGLFRVQPTGKVRMSTKSEPHIGMGVQHYGWFTSPLRRAADYINQKQLISLIDD
                     TAEPLYQNSDAELFAALRDFDAAYTAYADFQRQMEAYWSLVYLQQQGTSELTATILKE
                     DLVRIEGLPLVTRATGIPFDALPKSQALFKITELDAEKQFVSLNYIKAVALAGKTAGN
                     AV"
BASE COUNT          603 a          673 c          519 g          421 t
ORIGIN      
        1 gtactggttt ttgttaatcc actataacgc aagcaccgca aagacgcgcc aaccctcccc
       61 caaccttttt tcagacggca ttttcggtaa tctgctaaaa tcgcccgctt gagtttccac
      121 agaaaaatcc gaaaaatgaa tattttttac gaagagtccg gccaattcaa aatcgccgcc
      181 atcatccaaa aaaacgacgc tacctaccaa gtcgataccc cacacggcaa acgcaccaaa
      241 gtgaaggcga acaacgtctt taccgagttc gacggcgata tggcggcgtt tttggaaaac
      301 gcgcaggcac aggcggcgga tatcgacacc gatttattgt gggaagtgtg cggcgaagag
      361 gaatttaccg ctgaagccat agccgaagaa tattacggcc atgcgccgac caaaaccgag
      421 ctggcggcga ccttgattgc gctgtacgcc gcgccgatgt atttctacaa aaaagccaaa
      481 ggcgtgttca aagccgcgcc cgaagaaact ttaaaacaag cacttgccgc catcgaacgc
      541 aaaaaacagc aagacgcgca aatcgacgct tgggcagaag ccttgaaacg cggtgagatg
      601 ccgtctgaaa tcgcggcgga tttgaaaacc atcctgcacg cgcccgacaa gcagtcgctg
      661 acctacaaag cctttaccaa agccgccgac gcgctgaaaa cctctgccta cgaattggcg
      721 aaaaaaacgg gcggcattac gtccattccc caatacctgc aagacgggtt tgaaatcaaa
      781 tacttcccca aaggaacagg cttccccgac cttccgcttc ccgaaatgcc cgacctgccc
      841 aaggctgacg ttaccgcctt ttccattgac gacgaatcga ccaccgaagt ggacgacgct
      901 ttaagcctga ccgacttgga caacggcacg aagcgcgtcg gcatccacat cgccgcgccg
      961 tcgcttgcca ttaaacaagg cgacaaaatg aaaaaaaaca tcatggaacg cttgagcacg
     1021 gtttatttcc ccggcggcaa aatcacgatg ctgcccgaaa actggattgc cgcgttcagc
     1081 cttgatgcag gcgcacaccg ccctgccgtc agcatttatt tcgatgtgga cggcgagttc
     1141 aacgtcggcg cgccgacctg caaaatcgaa gcggtcaaca tctccgaaaa cctgcgtatc
     1201 caagccatcg aaccgcattt caacgccgaa gcgggcttgg acgaagcggg cgaaatgatg
     1261 ttcgcccacc atcaagacct gatttggttt tatcaattcg ccatcaccct gcaaaaagcg
     1321 cgcggcaaat acgagcccaa ccgtgcgccg caatacgatt acagcatcga attggataaa
     1381 gaagacaacg tatccgtcgt ccgccgcgaa cgcggctcac ccatcgatac gctggtcagc
     1441 gagatgatga ttcttgccaa cagcacttgg gcacaaatgc tccatgacaa cgacctgccc
     1501 ggcctcttcc gcgtccaacc caccggaaaa gtacgcatga gcaccaaatc cgagccgcat
     1561 atcggcatgg gcgtgcagca ttacggctgg tttacctcgc cgctgcgccg cgccgccgac
     1621 tacatcaacc aaaagcagct gatcagcctg atagacgaca ctgccgagcc gctgtatcaa
     1681 aacagcgatg ccgagctttt cgccgcactg cgcgactttg atgccgccta taccgcctac
     1741 gccgatttcc aacggcagat ggaagcctac tggagccttg tgtacctgca acagcaaggt
     1801 acaagcgagc tgaccgcgac catcctcaaa gaagacctcg tccgcatcga aggcctgccg
     1861 ctggtcacgc gcgcgaccgg tattccgttt gacgcgctgc ccaaatcaca ggcattgttc
     1921 aaaatcaccg aattagatgc cgagaagcag tttgtctcgc tcaactacat caaggcagtc
     1981 gcactcgcag gcaaaacggc aggcaatgcc gtctgaagcc cgatacggca aactccggca
     2041 aaaacagaaa cctgaatctc atcattccca cagtcgggaa tccggttttt tgggtttccg
     2101 ttgtttttcc gtttcaatga acttccaagc cgccgttccc gataaatacc cgcaatctaa
     2161 aatcccgtca ttcccacgca gatgggaatc taggtttatc cgcacagaaa ctgata
//
LOCUS       DAWHSK010000273         2192 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.273, whole genome shotgun sequence.
ACCESSION   DAWHSK010000273 DAWHSK010000000
VERSION     DAWHSK010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.273"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            30..515
                     /locus_tag="WIC03_001958"
     CDS             30..515
                     /locus_tag="WIC03_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884411.1"
                     /translation="MFVNEKDPYAALFAGLVFLTLPFALAVHDALAFVFGRTGLLVSV
                     SDGGFGWRGGWDGVVWFVYGVFAFLNLVVSAGLTKLAYKKMMRRHSRYALFLSGVAAC
                     AAAAVAWIFELLLGSGALGGLRGEAVLEYAFAVWLVAMLTLPKRLTRAPVQPVVFHRK
                     K"
     gene            611..1234
                     /gene="rsmG"
                     /locus_tag="WIC03_001959"
     CDS             611..1234
                     /gene="rsmG"
                     /locus_tag="WIC03_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEZ3884412.1"
                     /translation="MERKERLRAGIAALGLDISAAAQDRLLAYVDLLKKWNKTYNLTA
                     LRDEEKMIVHHLLDSLTLLPHIEGVQTMLDVGSGGGQPGIPAAVCRPDVQITLLDANT
                     KKTAFLQQAVIELGLDNVRVVSGRVEAVSDVRADVVTSRAFAELADFVSWTVHLLKDG
                     GYWAAMKGVYPQEEIGRLPQDVCVEKVQRLDVPGLEAERHIVILSKR"
     gene            1333..2106
                     /locus_tag="WIC03_001960"
     CDS             1333..2106
                     /locus_tag="WIC03_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEZ3884413.1"
                     /translation="MSANILAIANQKGGVGKTTTTVNLAASLASRGKRVLVVDLDPQG
                     NATTGSGIDKAGLQSGVYQVLLGDADVKSAAVRSKEGGYAVLGANRALAGAEIELVQE
                     IAREVRLKNALKAVAEDYDFILIDCPPSLTLLTLNGLVAAGGVIVPMLCEYYALEGIS
                     DLIATVRKIRQAVNPDLDITGIVRTMYDSRSRLVAEVSEQLRSHFGDLLFETAIPRNI
                     RLAEAPSHGMPVMAYDAQAKGAKAYLALADELAARVSGK"
BASE COUNT          422 a          503 c          761 g          506 t
ORIGIN      
        1 tcggtctgct tcggaagagg gggacggcga tgtttgtgaa cgagaaagat ccttatgcgg
       61 ctctgtttgc gggactggtg tttttgacgc tgccgtttgc gttggcggtg catgatgcct
      121 tggcatttgt gttcggacgg acggggctgc tggtgtcggt gtcggacggc ggattcggct
      181 ggcgaggagg ctgggacggc gtggtgtggt ttgtgtatgg cgtgtttgcg tttttgaacc
      241 tggtcgtgtc ggcgggcctg acgaaactgg cgtacaaaaa gatgatgcgg cggcattccc
      301 gttacgcgct gtttctgtcg ggcgtggcgg cttgcgcggc tgctgcggtc gcgtggattt
      361 tcgagctgct gcttggcagt ggggctttgg gcgggctgcg tggggaggcg gtgttggaat
      421 atgcgtttgc cgtgtggctg gtggcgatgc tgacgctgcc caaacgcctg acgcgcgcgc
      481 cggtgcagcc ggtggtgttt cacaggaaaa aataggttgg aacgggaaat gccgtctgaa
      541 ccccgacacg cggtttcaga cggcatgttt ttccgttaac attacgcctg aacatggaca
      601 ggatagggat atggaacgca aagaacgcct gcgtgcaggc atcgccgcat tgggtttgga
      661 tatttccgca gcggcgcagg acaggctttt ggcctatgtg gatttgttga aaaagtggaa
      721 caaaacctac aatctgaccg ccctgcgcga cgaggaaaaa atgattgtcc atcatctttt
      781 ggacagcctg acgctgctgc cccatatcga gggtgtgcaa acgatgctgg atgtcggttc
      841 gggcggcggt cagcccggca ttccggcggc ggtgtgccgt ccggatgtgc agataaccct
      901 tttggatgcg aatacgaaga aaacggcttt tttacagcag gcggttatcg agttgggatt
      961 ggacaatgtg cgcgtggtat ccggacgcgt ggaggcggtt tcggacgtgc gtgccgatgt
     1021 ggttaccagc cgtgcgtttg cagaactggc ggattttgtg tcgtggacgg tgcatctgtt
     1081 gaaagacggc ggctactggg cggcgatgaa gggcgtgtat ccgcaggaag aaatcggccg
     1141 cctgccgcag gatgtgtgcg ttgaaaaagt ccaaaggctc gacgtgccgg gcttggaggc
     1201 ggaacgccat atcgttatcc tgagcaagcg ttgagcgcgc ttcagacggc atgaatacct
     1261 tttttgtgcg gataaaggta aaattccgca ctgtttttct tttttcaaca tcagacggga
     1321 cacgggcggg acatgagtgc gaacatcctt gccatcgcca atcagaaggg cggtgtgggc
     1381 aaaacgacga cgacggtaaa tttggcggct tcgctggcat cgcgcggcaa acgcgtgctg
     1441 gtggtcgatt tggatccgca gggcaatgcg acgacaggca gcggcatcga caaggcgggt
     1501 ttgcagtccg gcgtttatca ggtcttattg ggcgatgcgg acgtgaaatc ggcggcggta
     1561 cgcagcaaag agggcggata cgctgtgttg ggtgcgaacc gcgcgctggc cggcgcggaa
     1621 atcgagctgg tgcaggaaat cgcccgggaa gtgcgtttga aaaacgcgct caaggcagtg
     1681 gcggaagatt acgactttat cctgatcgac tgtccgcctt cgctgacgct gttgacgctt
     1741 aacggcttgg tggcggcggg cggcgtgatt gtgccgatgt tgtgcgaata ttacgcgctg
     1801 gaagggattt ccgatttgat tgcgaccgtg cgcaaaatcc gtcaggcggt caatcccgat
     1861 ttggacatca cgggcatcgt gcgcacgatg tacgacagcc gcagcaggct ggttgccgaa
     1921 gtcagcgaac agttgcgcag ccatttcggg gatttgcttt ttgaaaccgc catcccgcgc
     1981 aatatccgcc ttgcagaagc gccgagccac ggtatgccgg ttatggctta tgatgcgcag
     2041 gcaaagggtg ccaaggcgta tcttgccttg gcggacgagc tggcggcgag ggtgtcgggg
     2101 aaataggtca atccaaatcg ggctgcccgt gcctttatgc tgtttggccg ggtgcgttaa
     2161 tatggcggat taaaataaaa atacttatat cg
//
LOCUS       DAWHSK010000274         2171 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.274, whole genome shotgun sequence.
ACCESSION   DAWHSK010000274 DAWHSK010000000
VERSION     DAWHSK010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.274"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(367..777)
                     /locus_tag="WIC03_001961"
     CDS             complement(367..777)
                     /locus_tag="WIC03_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884414.1"
                     /translation="MKIYNLDNIITSSALSLYKSTWVKLDLISCAKFIIEGTDFTFDW
                     DEGIENWIVFFHKNKVIGYLCAKLPFAILDNEQLKNQLYHKYPHVLTLQIKNIETEKI
                     SLNVNICRLTLCPSFHDEHDTLVCSVQDFYVNTV"
     gene            complement(1365..1748)
                     /locus_tag="WIC03_001962"
     CDS             complement(1365..1748)
                     /locus_tag="WIC03_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884415.1"
                     /translation="MMTEPHGYPSNNLPVKIINDIIYATQLVEDLVLGKIKIVDFLKS
                     YNNFYYWLGFDELPQSEKIKFLNYLNILSIHKEIQDKTVNRVYTDCFDIDKLHSLGRI
                     TTNECINGIKKIYQKNKLEFDNILK"
     gene            complement(1714..>2171)
                     /gene="mafB"
                     /locus_tag="WIC03_001963"
     CDS             complement(1714..>2171)
                     /gene="mafB"
                     /locus_tag="WIC03_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HEZ3884416.1"
                     /translation="GKKVELNPTKWDWVKNNGYKKPAVRLMQTVDGEMAGGNKPPKPS
                     TQQHSTHSDNNIGLPAPYVKPDTSISPTGTIYVDSKGNAIPTPIGGYITGSPDARFIQ
                     VRDSNGNLTGARIDDAHHANTHQDPRALTGHAHVPGVTNDDGTPWLPIK"
BASE COUNT          636 a          389 c          355 g          791 t
ORIGIN      
        1 gagttacccg tatgattgcc gtatggtcgg atacttgtat ccgacaaaaa tatttgacgc
       61 gtctatcgtt tccgaaatgc cgctgttgaa aaatgtcgga ttcgagaatc cgacctacgg
      121 ttgctgaaca atgttttgta gaacttgctc aaaatccatg tttcgccatt ttcctttata
      181 tatacctaat acagcacggg ttacgatttc gtttaagtca taaatagcca tttacgtcct
      241 ctgctaattt tcagcgagta gattgggtta ccaaacccaa caaaaaagca gcctgaaaaa
      301 tttcaggatg cttgttttat gttttttatc aatgaattag gttttaatcc agcctgcgct
      361 tgctagttaa acagtattta cataaaaatc ttgtactgaa caaacaagcg tatcatgttc
      421 atcatgaaat gatgggcata acgttaatct acaaatgttt acatttaaag atattttttc
      481 agtttcaata tttttaattt gtaaggttaa tacatgagga tatttgtgat atagctgatt
      541 ttttaattgt tcgttgtcta atatagcaaa aggtaatttt gcacacaaat aaccaataac
      601 tttgttttta tgaaaaaaaa ctatccaatt ttcaattcct tcatcccaat caaaagtaaa
      661 atccgttcct tctatgataa atttagcaca agatattaaa tctaacttaa cccaagtgga
      721 tttatacaga ctcaatgctg atgaagttat aatattatct aaattataaa ttttcataaa
      781 tcaacgttcc ttacctggtg ttatatgcac aggtctaatc ccatctttat tcggctttct
      841 acgataaatg ttaattatat gtgtcggttt tccgtctgta tagccaacta cgtcgttcgt
      901 tggaactttt gccttatttt gactatccca taaattatgt ttatctgcat atctagcagc
      961 atctaatgta gctttttccg catcaacatc tgatttaaat aaacttttgc tatctatctt
     1021 gtctagacta gatgcatcag gtttgtgtcg taaaaagcac tgtcctgtaa taggctcgcg
     1081 gcagcaagtt gtgcttccgg acttaatttt cctaaatcat taataccttg taaagtttgc
     1141 tgcgcagaga tgtaggtcgg atatttgtat ccgacaaaaa tatttgacgc atctatcgtt
     1201 tccgaaatgc cgctgttgga aaatgtcgga ttcgagaatc tgacctacgg ctgctgacta
     1261 ttttcttgtt tatataattc tacaactgca aactactatc aaatcttttt atctctggct
     1321 ctacactcag caagaatatt tttattttac aaaacatagg ctagttattt gagtatattg
     1381 tcaaattcta atttgttttt ttgatagatt ttttttatac cattaataca ttcattagtt
     1441 gtaattctac caagcgaatg taatttatca atatcaaaac aatcggtata aaccctattc
     1501 acagttttat cttgtatttc cttatgaata cttaatatat tgagatagtt tagaaatttt
     1561 attttctcag attgaggtaa ctcatcaaaa ccaagccaat aataaaaatt gttatatgat
     1621 tttaaaaaat caacaatttt tatttttcct aaaaccaaat cttcaactaa ctgggttgca
     1681 taaataatat catttatgat tttaacaggt aaattatttg atgggtaacc atggggttcc
     1741 gtcatcattt gtaactcctg gtacatgtgc gtgtcctgta agtgcgcgtg ggtcttgatg
     1801 ggtatttgca tgatgagcat catctattct ggctccagtc aagtttccat tactatctcg
     1861 gacttggata aaacgagcat caggcgaacc tgttatataa ccgcctattg gtgttggtat
     1921 tgcatttcct ttgctgtcta cataaattgt tcctgtcgga gaaatagatg tatcaggttt
     1981 aacatatggg gcaggtaagc ctatattgtt atcagagtgt gtagagtgtt gctgcgtact
     2041 tggtttcggt ggcttatttc ccccagccat ctccccatct acagtctgca taaggcgaac
     2101 agcaggtttt ttatagccgt tatttttaac ccaatcccat ttggtcgggt taagttctac
     2161 ttttttacct c
//
LOCUS       DAWHSK010000275         2162 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.275, whole genome shotgun sequence.
ACCESSION   DAWHSK010000275 DAWHSK010000000
VERSION     DAWHSK010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.275"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(81..1292)
                     /locus_tag="WIC03_001964"
     CDS             complement(81..1292)
                     /locus_tag="WIC03_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease"
                     /protein_id="HEZ3884417.1"
                     /translation="MNQLKLAVSGAQILFVAFGAMVLVPLLTGLNPALALLGAGLGTL
                     LFQITTKRKVPIFLGSSFAFIAPIIYSIGEWGLPSTMFGLFAAGFMYFVFAVLIRWRG
                     LAAVHKLLPPVVIGPVIMVIGLSVAVAASSMAMGQADGKQVIDYTDSLILSGFTFAVT
                     AIVSVFGSRMMKLIPILIGVASGYVLALLMGLVDTASIAHAPWFAVPHFETPQVNWQA
                     ALFMLPVAVAPAIEHIGGIMAIGNVTGKDYTKDPGLDKTLAGDGLGVCVAGLIGGPPV
                     TTYGEVTGAVMITKNSNPVIMTWAAVFAVCMAFFGKFNAFLASIPMPVMGGIMLLLFG
                     TIASLGVKTLIDAKVDLMLPKNLVIVSSVLTTGIGGMTLKLGSFSFAGVGLCAVLAIM
                     LNSLLPDPKES"
     gene            1515..>2162
                     /locus_tag="WIC03_001965"
     CDS             1515..>2162
                     /locus_tag="WIC03_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEZ3884418.1"
                     /translation="MTEKSHKKTAKGRAGSPSPTSARNKKADNGARGNKVSERLKAVK
                     ELQKTETKKARPEHVVNLIGDALWLMGLAATLYLAISLISFDMGDPSWSHSSPVVEDV
                     ANWGGLFGAYVADVGYYLFGWSFWWWIAAACVVLYKNFRLHAKQTENEAYNHKIAAAA
                     LFVLTVFSPVLEYFVLGGKYADSLPVGAGGMVGIRVGAVFAWLLGKSGSLLIILVV"
BASE COUNT          551 a          578 c          587 g          446 t
ORIGIN      
        1 tgcgctcttt gattcaaaat ctcaaataaa acggaaaatg ccgtctgaaa gatgttcaga
       61 cggcatttct atatcgacgg tcaggattct ttcggatcgg gcagcaggct gttcaacata
      121 atggcaagta cggcgcacaa gcccacgccg gcaaagctga agctgcccaa tttgagcgtc
      181 atgccgccga tgcccgtggt cagtaccgag ctgacgatga ccaggttttt cggcagcatc
      241 aaatcgactt tggcatcaat cagcgttttc acgcccaaag aagcaatcgt gccgaacagc
      301 agcagcataa tgccgcccat tactggcatc ggaatggaag ccaaaaacgc attgaatttg
      361 ccgaaaaacg ccatgcagac ggcaaaaacc gccgcccaag tcatgatgac ggggttgctg
      421 tttttggtaa tcatcaccgc acccgttact tcgccgtagg tcgtaaccgg cgggccgccg
      481 atcagacccg caacgcacac gcccaaaccg tcgcctgcaa gggttttgtc caagcccggg
      541 tctttcgtat agtctttccc cgtcacattg ccgattgcca tgatgccgcc gatgtgttcg
      601 atggcggggg cgacggcaac gggcagcata aacagtgccg cctgccagtt gacctgaggc
      661 gtttcaaaat ggggaacggc gaaccagggc gcgtgtgcaa tgcttgccgt gtccaccagt
      721 cccatcagca gtgccaaaac ataacccgaa gcgacaccga tcaagatagg aatcagcttc
      781 atcatcctgc tgccgaaaac cgatacgatg gcggtaacgg caaaggtaaa gccggaaaga
      841 atcagtgaat cggtatagtc gatgacctgt ttgccgtccg cctgacccat tgccatgctg
      901 cttgccgcca cggcaacaga cagaccgatg accatgatga cggggccgat gacgaccggc
      961 ggcagcagtt tgtgtaccgc cgccagtccg cgccaacgaa ttagcacggc aaacacaaaa
     1021 tacataaagc cggcggcaaa cagtccgaac atggtggaag gcagccccca ttctccgatg
     1081 gagtagataa tcggtgcgat aaaggcaaac gaagaaccga gaaaaatcgg cactttacgt
     1141 ttggttgtaa tttggaacag cagcgttccc aagcctgcgc ccaaaagcgc aagagccgga
     1201 ttcagaccgg tcagcagggg aaccagcacc attgcgccga atgccacaaa taaaatctgt
     1261 gcaccggaaa cggcaagttt cagttggttc ataccatctt ctttcaatcg gttcaaacgg
     1321 cacggattat actggtttgc cgagggtctg cgtaaaaata catacgtcgg gcaacagata
     1381 tgccgtctga aaggcaatgt ctgtctgttg caggatgaaa gcaggagaaa tggagtataa
     1441 tccgacactt tccatattca atccgcaagc cgtccggctt ccttatttca gacggcattt
     1501 tccggtaata cggcatgaca gaaaaatccc ataaaaaaac agcaaaaggc agggcgggaa
     1561 gcccgtcccc aacgtccgca cgcaacaaga aagccgacaa cggcgcacgg ggcaataaag
     1621 tttccgagcg actcaaggcg gtcaaagagt tgcagaaaac cgaaaccaaa aaggcgcgtc
     1681 ccgaacatgt cgtcaacctt atcggcgacg cactgtggct gatgggtttg gcggcaaccc
     1741 tgtatttggc gatttccctg atcagtttcg atatgggcga tccgtcttgg tcgcacagtt
     1801 cgccggttgt ggaagatgtc gccaattggg gcggactgtt cggcgcgtat gttgccgatg
     1861 tcggctatta tcttttcggc tggtcgttct ggtggtggat agcggctgcc tgcgtcgtgc
     1921 tgtataaaaa tttccgcctg cacgcaaaac agacggaaaa cgaggcatac aaccacaaaa
     1981 tcgctgccgc cgcgctgttt gtcctgacgg tcttcagccc cgtcttggag tattttgtgc
     2041 tgggcggaaa atatgccgac tccctgcctg tcggagcagg cggtatggtc ggcatacgcg
     2101 tcggcgcagt gtttgcgtgg ctgctgggga aatcgggcag cctgctgatt atcttggttg
     2161 tt
//
LOCUS       DAWHSK010000276         2122 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.276, whole genome shotgun sequence.
ACCESSION   DAWHSK010000276 DAWHSK010000000
VERSION     DAWHSK010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.276"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            123..1508
                     /locus_tag="WIC03_001966"
     CDS             123..1508
                     /locus_tag="WIC03_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HEZ3884419.1"
                     /translation="MDGWTQTLSAQTLLGISAAAIILILILIVKFRIHALLTLVIVSL
                     LTALATGLPTGSIVNDVLVKNFGGTLGGVALLVGLGAMLGRLVETSGGAQSLADALIR
                     MFGEKRAPFALGVASLIFGFPIFFDAGLIVMLPIVFATARRMKQDVLPFALASIGAFS
                     VMHVFLPPHPGPIAASEFYGANIGQVLILGLPTAFITWYFSGYMLGKVLGRTIHVPVP
                     ELLSGGTQDNDLPKEPAKAGTVVAIMLIPMLLIFLNTGVSALISEKLVSADETWVQTA
                     KIIGSTPIALLISVLVALFVLGRKRGESGSALEKTVDGALAPVCSVILITGAGGMFGG
                     VLRASGIGKALADSMADLGIPVLLGCFLVALALRIAQGSATVALTTAAALMAPAVAAA
                     GFTDWQLACIVLATAAGSVGCSHFNDSGFWLVGRLLDMDVPTTLKTWTVNQTLIALIG
                     FALSALLFSIV"
     gene            1528..2046
                     /locus_tag="WIC03_001967"
     CDS             1528..2046
                     /locus_tag="WIC03_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEZ3884420.1"
                     /translation="MTTHFVVMGVCGCGKTTAALSLQKHLGQCPHAEGDEFHTQANRD
                     KMGAGIPLTDEDRYPWLGNLRDWMTQQAQNGANHTIVTCSALKRGYRDILRGAEGKAA
                     FIHLSPPQDINLERMMSRKGHYMKAGMLDSQLEILEELGEGEYGVKIANPGTPEAVEA
                     DILNWVASENLL"
BASE COUNT          445 a          644 c          571 g          462 t
ORIGIN      
        1 gttcaaataa acaggaatat aacttcagac aaacaactta ccgcccgatt tgtgctatcg
       61 ttttcgcaca acttaaaaaa cctgacaatt ttgtactttt attacagaga aaggctttac
      121 aaatggacgg ctggacacag acgctgtccg cgcaaaccct gttgggcatt tcggcggcgg
      181 caatcatcct cattctgatt ttaatcgtca aattccgcat ccacgcgctg ctgacactgg
      241 tcatcgtcag cctgctgacg gctttggcaa ccggtttgcc cacaggcagc attgtcaacg
      301 acgtactggt caaaaacttc ggcggcacgc tcggcggcgt ggcgcttttg gtcggtctgg
      361 gcgcgatgct cggacgtttg gtcgaaacat ctggcggcgc acagtcgctg gcggacgcgc
      421 tgatccggat gttcggcgaa aaacgcgcac cgttcgcgct gggcgttgcc tcgctgattt
      481 tcggcttccc gattttcttc gatgccggac taatcgtcat gctgcccatc gtgttcgcca
      541 ccgcacggcg catgaaacag gacgtactgc ccttcgcgct tgcctccatc ggcgcatttt
      601 ccgtcatgca cgtcttcctg ccgccccatc cgggcccgat tgccgcttcc gaattttacg
      661 gcgcgaacat cggccaagtt ttgattttgg gtctgccgac cgccttcatc acatggtatt
      721 tcagcggcta tatgctcggc aaagtgttgg ggcgcaccat ccatgttccc gttcccgaac
      781 tgctcagcgg cggcacgcaa gacaacgacc tgccgaaaga acctgccaaa gcaggaacgg
      841 tcgtcgccat catgctgatt cccatgctgc tgattttcct gaataccggc gtatcggccc
      901 tcatcagcga aaaactcgta agtgcggacg aaacctgggt tcagacggca aaaataatcg
      961 gttcaacacc gatcgccctt ctgatttccg tattggtcgc actgtttgtc ttgggacgca
     1021 aacgcggcga aagcggcagc gcgttggaaa aaaccgtgga cggcgcactc gcccccgtct
     1081 gttccgtgat tctgattacc ggcgcgggcg gtatgttcgg cggcgttttg cgcgcttccg
     1141 gcatcggcaa ggcactcgcc gacagcatgg cggatttggg cattcccgtc cttttgggct
     1201 gtttccttgt cgccttggca ctgcgtatcg cgcaaggttc ggcaaccgtc gccctgacca
     1261 ccgccgccgc gctgatggct cctgccgttg ccgccgccgg ctttaccgac tggcagctcg
     1321 cctgtatcgt attggcaacg gcggcaggtt cggtcggttg cagccacttc aacgactccg
     1381 gcttctggct ggtcggccgc ctcttggaca tggacgtacc gaccacgctg aaaacctgga
     1441 cggtcaacca aaccctcatc gcactcatcg gctttgcctt gtccgcactg ctgttctcca
     1501 tcgtctgaca gacggaaagg atagtaaatg actacgcatt ttgtcgttat gggcgtatgc
     1561 ggctgcggca agaccaccgc cgcgctgtcc ctgcagaaac acctcggtca atgtccccat
     1621 gccgaaggcg acgagttcca cacccaagcc aaccgcgaca agatgggcgc gggtattccg
     1681 ctgaccgatg aagaccgcta tccgtggttg ggcaatctgc gcgactggat gacgcaacag
     1741 gcgcaaaacg gtgcgaacca caccatcgta acctgttccg ccctcaaacg cggctaccgc
     1801 gacattctgc gcggagccga aggcaaagct gctttcatcc acctcagtcc gccgcaagac
     1861 atcaacctcg agcgcatgat gtcgcgcaaa ggacattaca tgaaagcagg gatgctcgat
     1921 tcgcaactgg aaatcctcga ggaactgggc gaaggcgaat acggggtcaa aatcgccaac
     1981 cccggcacgc ccgaagcggt cgaagccgat attctgaact gggttgcctc ggaaaacctg
     2041 ctttgaagca atatgccgtc tgaagcccga cacaggatgg gtttcagacg gcataaacat
     2101 cgggaacaga atggattaca tt
//
LOCUS       DAWHSK010000277         2110 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.277, whole genome shotgun sequence.
ACCESSION   DAWHSK010000277 DAWHSK010000000
VERSION     DAWHSK010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.277"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(42..809)
                     /locus_tag="WIC03_001968"
     CDS             complement(42..809)
                     /locus_tag="WIC03_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEZ3884421.1"
                     /translation="MSSNKASFFTRLRRLCRLTVWLFKTGKNLRGIDGGCPESRNRAV
                     IELGRGALTALDIGLEVGRPAPEHPNGVLVAANHVSWLDIFAMSAVYPSSFIAKQEIK
                     SWPVLGKMGQNAGTVFINRNSRRDIEPINRAVYETLQRGQNVSFFPEARTSSGLGLLP
                     FKAALFQSAIDAGAKVLAVALRYYDETGKRTARPSYADVGLPTCLWRIVSMKKLTIKV
                     DFVCVADAAESEDRYALKDKIEESIRAVVADDADIAV"
     gene            complement(897..1724)
                     /gene="mutM"
                     /locus_tag="WIC03_001969"
     CDS             complement(897..1724)
                     /gene="mutM"
                     /locus_tag="WIC03_001969"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEZ3884422.1"
                     /translation="MPELPEVETTLRGIAPHIEGKTVEAVVLRQLKLRWQINPDLGEI
                     LSGRQVLSCGRRAKYLLIRFQTGVLLIHLGMSGSLRIFTPSDGRIGRPDRHDHVDIVF
                     SDGTVMRYRDPRKFGAILWYEGIEEHHPLLEKLGPEPLSEAFCTDYLYVRLKAQKRAV
                     KLALMDNAVVVGVGNIYANESLFRAGISPHRPANRLKKKECALLVETVKAVLRRAIET
                     GGSTLRDFVDSDGKSGYFQQEYTVYGRHNQPCPQCGGLVVKETLGQRGTFYCPNCQK"
     gene            complement(1776..>2110)
                     /locus_tag="WIC03_001970"
     CDS             complement(1776..>2110)
                     /locus_tag="WIC03_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEZ3884423.1"
                     /translation="LWGFSRWFDGERLPEKRFCLPLPELKRLLADAGFDIELGKFMVY
                     LPPVSSLGQIRFWQFMEKAGDRWWPQCAAVYGLVLVKRAAGVTPLPAWDGYLGGKALA
                     AGAARIAD"
BASE COUNT          484 a          670 c          504 g          452 t
ORIGIN      
        1 ccgcaaaagc gaatcatact gccacattcc ggcaaccggt ttcagacagc gatatccgca
       61 tcgtcggcga caacggcacg gatgctttct tcgattttat cttttaaagc ataacggtct
      121 tcgctttccg ccgcatccgc cacgcaaacg aaatcgactt ttatcgttaa ttttttcata
      181 gacacaatgc gccacaggca ggtcggcaaa ccgacatcgg catatgaggg acgagccgtc
      241 ctttttcccg tttcgtcata ataacgcagc gcgaccgcca aaacctttgc ccccgcatca
      301 atggcggatt ggaacagcgc ggctttgaac ggcaggagcc ccagtccgga ggaagtccgc
      361 gcttcgggga aaaaactgac gttttgaccg cgttgcaagg tttcgtagac ggcgcggtta
      421 atcggttcga tgtcgcgccg cgaattgcgg ttgatgaaca ccgttcccgc gttctgcccc
      481 atcttgccca ataccggcca gcttttgatt tcctgcttgg cgataaagct gctcggataa
      541 accgcgctca tcgcgaaaat atccagccag gacacgtggt tggcggcaac caagacaccg
      601 ttcggatgtt cgggtgcggg cctgcccacc tccaatccga tatccaaagc cgtcagagcc
      661 cccctgccca actcgattac cgcccgattg cgcgactcgg ggcagccgcc gtcaataccg
      721 cgcaggtttt tgccggtttt gaatagccag accgtcaagc ggcacaagcg gcgcagacgt
      781 gtaaaaaatg aagctttatt tgaagacatg aacaattctt tcccgataat cggtgtttgt
      841 tgttgaaacg ttcggacggc ataccgataa aatgccgtct gaaaccgttt tccgccctat
      901 ttctgacaat tcgggcagta aaacgtgccg cgctgcccca aagtttcttt cacaaccaaa
      961 ccgccgcact gggggcacgg ctgattgtgc cgcccgtaca ccgtatattc ctgttggaag
     1021 tagccgcttt tgccgtcgct gtccacaaaa tccctcaagg tactgccgcc cgtttcaatg
     1081 gcgcgccgca acaccgcttt gacggtttca accaaaagcg cacactcttt ctttttcaga
     1141 cggttggcag gacggtgggg cgaaatgccc gctctgaaca ggctctcatt ggcataaata
     1201 ttgcccacac cgaccacgac cgcattgtcc atcagggcaa gtttgaccgc gcgcttctgc
     1261 gccttcagcc tcacatacag ataatccgtg caaaatgcct ccgacaaagg ctccggcccc
     1321 agtttttcca acagcggatg atgttcttcg attccctcat accaaagtat cgcgccgaac
     1381 tttctcggat cgcggtaacg catgaccgtg ccgtctgaaa acacaatatc gacgtgatcg
     1441 tgtctgtccg gcctgccgat acgtccgtcc gacggcgtaa aaatccgcaa gctgcccgac
     1501 atccccaagt gaatcagcag cacgcccgtt tgaaagcgga taagcaggta tttcgccctc
     1561 ctgccgcagg acaacacctg ccggccggac aaaatctccc ccaaatcggg attaatctgc
     1621 cagcgcagct tcaattggcg caataccacg gcttccaccg ttttcccttc aatatgcggc
     1681 gcgatgccgc gcaacgtcgt ttccacttcc ggcaattcag gcataacccc tcccgacatt
     1741 tcttctgaca gatgccgtct gaaagacggc tgtccctaat ccgcaatcct tgccgcaccc
     1801 gccgcaaggg ctttgccgcc caaatacccg tcccacgcgg gcaggggcgt tacgcccgcc
     1861 gcccttttga ccaaaaccag accgtacact gcggcacatt gcggccacca acggtcgccc
     1921 gccttttcca taaactgcca aaagcgtatt tgcccgagcg acgaaacggg cggcagatac
     1981 accataaatt tcccaagttc aatatcgaag ccggcatcgg caagcagcct tttcaactcg
     2041 ggcagcggca gacaaaaccg tttttccggc aggcgttcgc cgtcaaacca acggctgaat
     2101 ccccagagcg
//
LOCUS       DAWHSK010000278         2105 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.278, whole genome shotgun sequence.
ACCESSION   DAWHSK010000278 DAWHSK010000000
VERSION     DAWHSK010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.278"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            54..1094
                     /gene="murB"
                     /locus_tag="WIC03_001971"
     CDS             54..1094
                     /gene="murB"
                     /locus_tag="WIC03_001971"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEZ3884424.1"
                     /translation="MQPIRYRTDLTPYNTFGLRAQARAFIALEHADELCDIVRLPEFD
                     RDTVLWLGGGSNILLMQDYAGLVVHMENKGIREIARSDGIVRIEAQAGEIWHDFVLHT
                     VALGLNGLENLSLIPGTVGASPVQNIGAYGVEAKDVIHSVRCFDLDTEAFVELANADC
                     RFAYRESLFKQEGKGRYVIVSVVFALKEHFVPNLGYGDLAAAVAERCAGREITAKEVS
                     DAVCAVRRRKLPDPGILGNVGSFFKNPVVGAGKAAALLQQHPDMPHYPQPDGSVKLAA
                     GWLIDQCRLKGFQIGGAAVHDKQALVLVNKNNASANDVRQLAQHIKFTVFAQFQVELH
                     AEPNWLPASFSL"
     gene            1106..1756
                     /locus_tag="WIC03_001972"
     CDS             1106..1756
                     /locus_tag="WIC03_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEZ3884425.1"
                     /translation="MPVTRPAKINTYTRIIDASLALFNEEGERNISTNHIAAHLGISP
                     GNLYYHFRNKDEIIVQLFKRYSEALLAYLNEAVLPSDVEDSINYMAGIYDVMWEYRFL
                     FSDVNTLLARSAELLGEHNTFTQAKVSPLLVNLLTQLNGLNIIQADQTAMNDLAVNMW
                     MVTKYWFDFDSSLRGRTKLTEDSKARGIRRTLSLLRPYLLPEHREEYDRKIGNGNP"
     gene            1811..>2105
                     /locus_tag="WIC03_001973"
     CDS             1811..>2105
                     /locus_tag="WIC03_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEZ3884426.1"
                     /translation="MCAVPPPHISITGLGYLGLPLAQKFYQHSSRVAAIKRSLTSDDI
                     NLPIHLDTFDLGSTDAFQTALWRHHADKPVWFFLLPPSSLTHYADTVKQWAELA"
BASE COUNT          494 a          658 c          525 g          428 t
ORIGIN      
        1 aaacgatggg ctgaagcccg ccactggcaa tactcatttt aaagttcaaa cccatgcaac
       61 ccatccgata ccgaaccgac cttaccccct acaacacttt cggcctgcgc gcccaagccc
      121 gggcctttat cgcgctcgaa catgccgacg agttgtgcga catcgtccga ctgcccgagt
      181 tcgaccgcga tactgtttta tggctgggcg gcggcagcaa catccttttg atgcaggatt
      241 acgccggact ggtcgtacac atggaaaaca aaggcatacg cgagattgcg cgttcagacg
      301 gcattgttcg catcgaagcg caggcgggcg aaatctggca cgattttgtc ctgcacaccg
      361 tcgcgctggg cttgaatggg ctggaaaacc tgagcctgat tccgggtacg gtcggcgcgt
      421 cgcccgtgca gaacatcggc gcatacggcg tagaggcgaa agatgtgatt cacagcgtgc
      481 gctgctttga tttggatacg gaggcctttg tcgagcttgc caatgccgac tgccgcttcg
      541 cctaccgcga aagcctgttc aagcaggaag gtaaagggcg ttatgtgatt gtttcggttg
      601 tgtttgcatt aaaagagcat tttgtgccga acttgggcta cggcgatttg gcggcagcgg
      661 ttgccgaacg gtgcgccggc agggaaataa cggcgaaaga agtttccgat gcagtgtgcg
      721 ccgtccgccg ccgcaaactg cccgacccgg gcattttggg caatgtcggc agtttcttta
      781 aaaaccccgt cgtcggcgcg ggaaaagccg ccgccctgtt gcagcagcat cccgatatgc
      841 cgcattatcc acagccggac ggttcggtca aacttgctgc cggctggctg atcgaccaat
      901 gccgtctgaa aggcttccaa atcggcggcg cggcggtaca cgacaaacag gcattggttt
      961 tggtaaacaa aaacaacgcc tcggcaaacg atgtccggca attggcgcaa cacatcaaat
     1021 ttacagtatt tgctcagttt caggtagaat tgcacgccga acctaattgg ctgcctgctt
     1081 cgttcagcct gtaaatccaa ggagtatgcc cgtgacccgc cccgccaaaa tcaatactta
     1141 cacacgcatc atcgacgcca gccttgcgct cttcaacgag gaaggcgagc gcaacatcag
     1201 caccaaccat attgccgccc acttgggcat cagtccgggc aacctctatt accacttccg
     1261 caacaaagac gaaatcatcg tccaactgtt caaacgttac agcgaagccc tgcttgccta
     1321 cctgaatgaa gctgtgttgc cgtctgatgt ggaagactcc atcaactata tggcgggcat
     1381 ttacgacgtg atgtgggaat accgcttcct attcagcgac gtgaacaccc tgcttgcacg
     1441 cagtgccgaa ctattaggcg aacacaatac tttcacccaa gccaaagtct ccccgctctt
     1501 ggtcaacctg ctgacccagc tcaacggtct gaacatcatc caagccgacc aaaccgctat
     1561 gaacgatctc gccgtcaata tgtggatggt tacgaaatac tggttcgact tcgacagctc
     1621 cctgcgcggc cgcaccaagc tgaccgaaga ctccaaagca cgcggcatcc gccgcacctt
     1681 aagcctcctt cgtccttatc ttttgcccga acaccgcgag gaatacgacc gcaaaatcgg
     1741 caacggcaac ccgtaaaccc acagcgtccg aatcagcctt cagacggcac ttacccgacg
     1801 gtatcagcga atgtgcgcag tccccccgcc ccatatctcc atcacaggtc taggttacct
     1861 cggcctgccg ctggcacaaa agttttacca acacagcagc cgcgtcgccg ccataaaacg
     1921 cagcctgact tcggacgaca tcaacctgcc catacacctc gacaccttcg acctcggcag
     1981 caccgatgcc tttcagacgg cattgtggcg gcaccacgcc gacaaacccg tctggttctt
     2041 cctgctgccg ccctcctcgc tgacgcatta cgccgacacc gtcaaacaat gggcagaact
     2101 tgccc
//
LOCUS       DAWHSK010000279         2095 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.279, whole genome shotgun sequence.
ACCESSION   DAWHSK010000279 DAWHSK010000000
VERSION     DAWHSK010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.279"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(295..786)
                     /locus_tag="WIC03_001974"
     CDS             complement(295..786)
                     /locus_tag="WIC03_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4760 domain-containing protein"
                     /protein_id="HEZ3884427.1"
                     /translation="MFWQLTVVSVTAVIALGTIFINKKTSKQKATLDVILNDYQDAQF
                     VEADNHISPYIRGTAVDDNNARIDLYEIYQNKGGQWEKERGHLLTVINRHEFYACEIN
                     SGVLDEDLFKRLHCTNFIKLWNAVSPLVMKIREEERKDTIFRELEILVALWKANPLKA
                     SDL"
     gene            complement(1038..1208)
                     /locus_tag="WIC03_001975"
     CDS             complement(1038..1208)
                     /locus_tag="WIC03_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884428.1"
                     /translation="MPSEIQTAFLLKGFSSTALHKGGFFMPSESPSDGIGLHGRSSPA
                     FKQERMSRMPDF"
     gene            complement(1224..1592)
                     /locus_tag="WIC03_001976"
     CDS             complement(1224..1592)
                     /locus_tag="WIC03_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEZ3884429.1"
                     /translation="MIDGELVALIHQTVLADEAGLKGRADMARLDGALSRIANWRQYE
                     NLEDIYEIAALYAQAIAKAHAFPDGNKRTALLTMLTYLDLQGISIAADQGLDDLIVSL
                     AAGETDFKQLAETLRRLDKE"
BASE COUNT          527 a          471 c          449 g          648 t
ORIGIN      
        1 tacttgtgca gaatacgcca aaagccgtcc gaactgtttc ggacggcttt tgtactgtat
       61 ttgcgccttc aggcaatatt ttgttatcca ttttcaagat gcaaaagctt tctaattgct
      121 tgatgtcgga tttcggttgt ttagggatac aaaaccaagt aaactaaaac tgtttgattg
      181 gaaaatgctc cgcaaggaga aaattatgtt caaaaaatca ctttataagg ctgctttggc
      241 gtatttcggc gattgcgtgg ctgcccatat ttcagaacag ttttgactgt ttattcacaa
      301 atcagatgcc tttagggggt ttgctttcca taatgcaacc aaaatttcca actctctaaa
      361 tattgtgtct ttgcgttctt cttcgcgtat tttcataaca agaggcgaaa ctgcattcca
      421 caattttatg aagttggtgc aatgcagccg tttaaacaaa tcctcatcca atactcccga
      481 gttgatttcg cacgcataaa actcgtgccg attgattacg gtaagtaaat gccctctctc
      541 tttttcccat tgtccgccct tattttgata aatttcatac aggtcgatcc gcgcgttgtt
      601 gtcgtcaact gccgtgccgc gaatataagg cgaaatatga ttgtcggctt ctacaaattg
      661 tgcatcttgg taatcattca aaataacatc taatgtcgcc ttttgctttg aagttttctt
      721 attgatgaat attgtcccca gtgcaatgac ggcggttact gaaacaacgg tcagttgcca
      781 aaacatcagc cattcggccg tccctcatag gttaaactgt acgctgtgcc tgaatatgta
      841 aaataaagtg aatacagata caaacaagga ggttagggca attactgaca accaacctcc
      901 ttgttttatt tgtttccgca ccttaccagc cattaaaacc ctcatttttg tatttcatcg
      961 tatttcctta atggttgtaa ccttgcttgt cgatatctgt atattaatgc cgtgcgcccg
     1021 ttctttcaag gggtgattta aaaatcaggc atccttgaca tcctctcctg cttaaaggcg
     1081 ggggaactcc tgccgtgtaa accaatgccg tctgaagggc tttcagacgg cataaaaaaa
     1141 ccgcctttgt gtaaagcggt tgaagaaaag cctttcaata aaaatgccgt ctgaatttca
     1201 gacggcattg ttgtcggata tgcctattcc ttatccagtc ggcgcagggt ttcggcgagc
     1261 tgtttgaagt cggtttcccc cgccgccaaa ctgacaatca agtcgtcaag cccttggtcg
     1321 gcggcaatgc tgatgccctg caaatccaga taggtcaaca ttgttaaaag cgcggtgcgc
     1381 ttgttgccgt cgggaaaggc gtgggctttg gctatggctt gtgcataaag ggcggcgatt
     1441 tcgtagatgt cctcaaggtt ttcatactgc cgccagttgg caatccgcga caatgcgccg
     1501 tcgagccgcg ccatatccgc ccgccctttc aaacccgctt catccgccaa tacggtttga
     1561 tggataagcg cgaccagttc gccgtctatc atttgtcggc aagtgccttg acggcttttt
     1621 gatgggtttt ggcaatgcgg cgggcggcgg caagcagtac gcgtttgcct gcttcgcctt
     1681 taagctcgat tttgacgggg cgtgtatttt ggttttgcat cgctgctcct tgtctgtttg
     1741 cgggcatttt agcttttttc cggcagcttg ggaaatgccg tctgaaaaca cttcagacgg
     1801 cattcttcta atagtgtaat gcaatagtta ctccaagatt tttgtatatg gctgttttta
     1861 tggtaaatta aattttcaca aagaataaat atatttaaat caataaaata aaatttagtc
     1921 gaatcccacc gtttccgcca gaaaacaaaa ccgccctgat tcagggcggt tctttttggc
     1981 gcgaatctgc acaattacca taaaagaatc attggattat gcttattttc cgtaacgccc
     2041 tgcaataaac cgcaatcggc agatgctttg atcacagttt tatggtaatt tatat
//
LOCUS       DAWHSK010000280         2089 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.280, whole genome shotgun sequence.
ACCESSION   DAWHSK010000280 DAWHSK010000000
VERSION     DAWHSK010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.280"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(133..396)
                     /locus_tag="WIC03_001977"
     CDS             complement(133..396)
                     /locus_tag="WIC03_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884430.1"
                     /translation="MDKEIKICPRCEQGYLYHAKPKYFSGEVILCDECYAMWLGDMKI
                     FYGQYGKDFYDYHEFMKDKGIEEINMWEGELFDHPYYEDEKFK"
     gene            complement(415..>2089)
                     /locus_tag="WIC03_001978"
     CDS             complement(415..>2089)
                     /locus_tag="WIC03_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HEZ3884431.1"
                     /translation="DAKIILKGIVNRIQSEEKLETNSTVWQKQAGRGSTIETLKLPSF
                     ESPTPPKLSAPGGYIVDIPKGNLKTEIEKLAKQPEYAYLKQLQVAKNINWNQVQLAYD
                     RWDYKQEGLTEAGAAIIALAVTVVTSGAGTGAVLGLNGAAAAATDAAFASLASQASVS
                     FINNKGDVGKTLKELGRSSTVKNLVVAAATAGVADKIGASALNNVSDKQWINNLTVNL
                     ANAGSAALINTAVNGGSLKDNLEANILAALVNTAHGEAASKIKQLDQHYIVHKIAHAI
                     AGCAAAAANKGKCQDGAIGAAVGEIVGEALVKNTDFSRMSATEIEKAKANITAYAKLV
                     AGATVGVTGGNVDVAANASETAVKNNALDIIWDIGNLVWDGGKWIYAKSIGDKQMARE
                     AAIDFGVDAAAAAVPFVPAGATKISRGGAYVLKAGDEAVDTAKAIQEIQKQTGIKLTY
                     DKVNKVWTTPAGLDYGLDAKHGNRIKHVLAHTIPNPNKPVHSVFNVSRKEVLPLVDEA
                     WRMKGNPLPNDSSVYLVDMKKPIGTKGETKVRIVVQKGTNKIISAYPQK"
BASE COUNT          488 a          530 c          429 g          642 t
ORIGIN      
        1 atgccttcgg ctgccagcag ttgcattgag gccgtctgaa aaacggatat tcagacggcc
       61 tattgctact ttaaataata tttgggttta aaatatcatc gtatttccca aattcgggga
      121 acagaaaatc aattatttaa atttttcatc ctcataatat gggtgatcaa aaagctctcc
      181 ttcccacata tttatttctt ctatgccttt atctttcata aactcatgat aatcataaaa
      241 atcttttcca tattgtccgt aaaaaatttt catatcccca agccacatag cataacattc
      301 atcgcataaa atgacctccc cagagaaata tttaggcttt gcatgataaa ggtagccttg
      361 ctcacatctt gggcaaattt taatttcttt atccataaga gattcctttc ttaattattt
      421 ctgaggatat gcagaaatga ttttatttgt tcctttttgc acaacaatcc gcacttttgt
      481 ttctcctttt gttccaatag gtttcttcat atctacaaga tatacggatg aatcatttgg
      541 caaaggattt cctttcattc tccaagcttc atcaaccaaa ggcaaaactt ctttacggga
      601 cacattaaaa acagaatgaa caggtttgtt tggatttgga attgtatggg ctaaaacatg
      661 tttaatccta ttaccatgct tagcatctaa cccataatct aaccccgccg gtgttgtcca
      721 aaccttatta accttatcat aagtaagctt gattccggtc tgcttctgaa tttcctgtat
      781 ggctttagcc gtatcaactg cttcgtctcc cgccttcaga acataagccc cgcctcggct
      841 gattttagtc gcacctgccg gaacaaaggg aacggcagct gcggcggcat ccacaccaaa
      901 atcaatcgcc gcttctcgag ccatctgctt atcgccaata gatttggcgt aaatccattt
      961 accgccgtcc catacgaggt tgccgatatc ccaaataata tctaatgcat tatttttaac
     1021 agctgtttcg gaagcatttg ccgccacatc aacattgcct cctgtaacac ctacagtcgc
     1081 tccggctacc aattttgcat acgcagtaat attcgcttta gctttttcga tttcggtcgc
     1141 actcatacga ctgaaatcag tattcttaac caaagcctca ccaacaatct caccgactgc
     1201 agcgcctatc gcaccatcct gacacttgcc cttattcgcc gccgctgccg cacagcccgc
     1261 tatggcatgg gcaatcttgt ggactatgta gtgctgatcc aactgtttga ttttactggc
     1321 tgcttctcca tgcgcggtat tgaccaaagc cgcaaggata ttcgcttcca gattgtcttt
     1381 caggctgccg ccgttgacag cggtattaat cagtgcggca ctgcccgcat tggctaggtt
     1441 gacggtcagg ttgttgatcc actgcttatc gctgacattg ttcagtgccg aagcgccgat
     1501 tttgtcggct acgcctgcgg tagcggcggc aaccaccaga tttttcaccg tgctgcttct
     1561 gcccagctct ttcagggttt tgccgacatc gcctttgttg ttgatgaacg atacggaagc
     1621 ctggctggcc aaagaggcga atgctgcatc ggttgcggcg gcggccgcac cgtttaatcc
     1681 caatacggct ccggttcctg cgcctgaggt gaccacggta acggccagtg cgataatcgc
     1741 cgcacctgct tcggttaagc cctcctgttt gtagtcccat ctgtcgtaag caagctgcac
     1801 ctgattccag ttgatgtttt tcgctacttg gagctgtttc agataggcat actcgggctg
     1861 tttggccagc ttttcgattt cggttttcag attgcctttc ggaatgtcga cgatatagcc
     1921 gccgggtgcg gacagtttgg gcggagtagg gctttcgaag ctgggcagtt ttagcgtttc
     1981 gatagtgctg ccgcgtccgg cctgtttctg ccatacggtt gagttggttt ctaatttttc
     2041 ttccgactgg atacggttca caatgccttt gaggataatt ttcgcatcg
//
LOCUS       DAWHSK010000281         2077 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.281, whole genome shotgun sequence.
ACCESSION   DAWHSK010000281 DAWHSK010000000
VERSION     DAWHSK010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.281"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..348)
                     /locus_tag="WIC03_001979"
     CDS             complement(<1..348)
                     /locus_tag="WIC03_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEZ3884432.1"
                     /translation="MEHKLPQLPYELDALSPHLSKETLEFHYGKHHQTYITNLNNQIK
                     GTEFENLPLEEIVKKSSGGVFNNAAQTWNHTFYWLGFTPKGQGKPVGELAAAIDAKWG
                     SFEKFQEAFNACAA"
     gene            511..1917
                     /gene="dnaB"
                     /locus_tag="WIC03_001980"
     CDS             511..1917
                     /gene="dnaB"
                     /locus_tag="WIC03_001980"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEZ3884433.1"
                     /translation="MNDYTAMPSEDREIGALSLPPHSMEAEQSVLGGLMLENPAWDRI
                     ADVVSGEDFYRHEHRLIFRSIAKLINESRPADVITVQEDLQRNEELEAAGGFEYLITL
                     AQNTPSAANIRRYAEIVRERSIMRQLAEVGTEIARSAYNPQGRDAGQLLDEAENKVFQ
                     IAESTAKSKQGFLEMPDLLKEVVQRIDMLYSRDNPDEVTGVPTGFIDLDKKTSGLQPG
                     DLIIVAGRPSMGKTAFSINIAEHVAVEGRLPVAVFSMEMGGAQLVMRMLGSVGRLDQS
                     VLKTGRLEDEHWGRLNEAVVKLSDAPVYIDETPGLTALELRARARRLARQFNNKLGLI
                     VIDYLQLMAGSGRSDNRASELGEISRSLKALAKELQVPIIALSQLSRTVESRTDKRPM
                     MSDLRESGAIEQDADLIMFMYRDEYYNQDSPMKGLAECIIGKHRNGPVGKIFLTWTGQ
                     FTKFDNAAYIPEEAKIED"
BASE COUNT          514 a          533 c          550 g          480 t
ORIGIN      
        1 ggcagcgcag gcattgaacg cttcttggaa tttctcgaag ctgccccatt tcgcgtcgat
       61 ggcggcggcc agttcgccga caggtttgcc ttgacctttg ggcgtgaaac ccagccagta
      121 gaaggtgtgg ttccaagttt gtgccgcgtt gttgaacacg ccgcccgaag attttttcac
      181 aatctcttcc aaaggcaggt tttcaaattc ggtgcctttg atttgattgt tcaggttggt
      241 gatgtaggtt tgatggtgtt tgccgtagtg gaattccaaa gtctctttgc tcagatgcgg
      301 ggacaatgcg tccagttcat aaggcagttg cggcagctta tgttccattt tgtgctccta
      361 atattgtttt aaatgttgta ttttggcagt gttgctgcaa atagctcagc agcgcacata
      421 ttttacctgt tttgagggat ggaaaccaat taaacctgct ttacgctata atagaagatt
      481 gcaatttcgg cacgacagat aggatgtacc atgaacgatt acacagccat gccgtctgaa
      541 gaccgtgaga tcggcgcatt gtcgcttccg ccgcactcaa tggaggcgga acaatccgtt
      601 ttgggcgggt tgatgctgga aaatccggca tgggacagga ttgccgatgt ggtttcggga
      661 gaggatttct accggcatga acaccgcctg attttccgtt ccattgccaa attaatcaat
      721 gagagccgtc ccgcagatgt gattacggtt caggaagatt tgcagcggaa cgaagaattg
      781 gaagcggcag gcggattcga atacctgatt acgctggcgc aaaacacccc gtctgccgcc
      841 aacatccgcc gctacgccga aatcgtgcgc gagcgttcca ttatgcgcca actcgccgaa
      901 gtggggacgg aaatcgcccg cagcgcatac aatccgcaag gcagggacgc ggggcagctt
      961 ttggacgagg cggaaaacaa agtattccaa atcgccgaaa gcaccgccaa atccaagcag
     1021 ggctttttgg agatgcccga tttgctgaaa gaagtcgtac agcgcatcga tatgctctac
     1081 tcgcgcgaca atcccgatga agttaccggc gtgccgacgg ggttcatcga cctcgacaaa
     1141 aaaacctcgg gtctgcaacc cggcgacctg attatcgttg ccggtcgtcc gtctatgggt
     1201 aagaccgcct tttctatcaa tatcgccgaa cacgttgccg tagaaggcag gctgcccgtt
     1261 gctgttttct cgatggaaat gggcggggcg caactggtca tgcgtatgct cggctcggtc
     1321 ggacggttgg atcaaagcgt tttgaaaacc ggcaggctcg aagacgaaca ctggggtcgc
     1381 ctgaacgaag cagtcgtcaa actctccgac gcgcccgtgt acatcgacga gaccccgggt
     1441 ctgaccgcgc tcgaactgcg cgcccgtgcc cgccgtctcg cccgtcaatt taacaataag
     1501 cttggattga ttgtcatcga ctacctgcaa ctgatggcag gatccggccg ttccgacaac
     1561 cgagcttcgg agctgggaga gatttcacgt tcgctcaaag cgttggcgaa agaattgcaa
     1621 gtccccatca tcgccctgtc gcaattgagc cgcacggtcg aatcgcgtac cgacaaacgc
     1681 cccatgatgt ccgacctccg cgaatccggc gcaatcgagc aagatgccga cctgattatg
     1741 ttcatgtacc gcgacgaata ctacaaccag gactcaccca tgaaaggcct tgccgaatgt
     1801 atcatcggca aacaccgcaa cggtcccgtc ggtaaaatct tcctcacatg gacgggacaa
     1861 ttcaccaaat tcgacaatgc tgcctatatt cccgaggagg caaagataga agattaaatg
     1921 gctatataaa aatttattag gcgaaatcag gcaaaatcgt ttaaaatcat gcttagagat
     1981 tgccacgcgg tcttgaggca tttttgcatt cagcccgcat ataattgaaa atatagtgga
     2041 ttaacaaaaa tcaggacaag gcgacgaagc cgcagac
//
LOCUS       DAWHSK010000282         2073 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.282, whole genome shotgun sequence.
ACCESSION   DAWHSK010000282 DAWHSK010000000
VERSION     DAWHSK010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.282"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(392..1315)
                     /locus_tag="WIC03_001981"
     CDS             complement(392..1315)
                     /locus_tag="WIC03_001981"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEZ3884434.1"
                     /translation="MLQRTLAKSISVTGVGLHSGERVALTLHPAPENSGISFRRTDLD
                     GEMGEQIKLTPYLINDTRLSSTIVTDKGVRVGTIEHIMSALSAYGIDNALIELNAPEI
                     PIMDGSSLPFIYLLQDAGVVDQKAQKRFLKILKPVEIKEAGKWVRFTPYDGFKVTLTI
                     EFDHPVFNRSSPTFEIDFAGKSYIDEIARARTFGFMHEVEMMRAHNLGLGGNLNNAIV
                     IDDTDVLNPEGLRYPDEFVRHKILDAIGDLYIVGHPIVGAFEGYKSGHAINNALLRAV
                     LADETAYDRVEFADSDDLPDAFHELNIRTCG"
     gene            1821..1943
                     /locus_tag="WIC03_001982"
                     /pseudo
     CDS             1821..1943
                     /locus_tag="WIC03_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
BASE COUNT          554 a          531 c          483 g          505 t
ORIGIN      
        1 ccgggattct agattcccac tttcgtggga atgacggcat cagtctgccg tttacagcac
       61 ggtttcttta gattttacgt tctagattcc cgcctgcgcg ggaatgacga atccatccat
      121 acgaaaacct gcaccacgtc attcccacga acctacatcc cgtcattccc acaaaaacag
      181 aaacctcaaa tcccgtcatt cccgcgcagg cgggaatcca gacttgtcgg tgcggacgct
      241 tatcggataa aacggtttct tgagattccg cgtcctggat tcccactttc gtgggaatga
      301 cgggatttga ggtttctgtt tttggttttc tgtttttgta ggaatgatga aattttaagt
      361 tttaggaatt taccggaaaa aatagaaagc gttatccaca agttctgatg ttcagctcgt
      421 gaaatgcgtc gggcaaatca tcgctgtcgg caaattccac ccggtcgtaa gccgtttcgt
      481 ctgccaaaac cgcgcgcaag agtgcgttgt tgatggcgtg tcccgatttg tagccttcaa
      541 acgcgccgac aatcggatgt ccgacgatat acaaatcacc gatggcatca aggattttgt
      601 ggcgcacaaa ctcatcggga tagcgcaagc cttcaggatt caggacatcc gtgtcgtcaa
      661 tcacgatggc gttgttcaaa ttgccgccca aacccagatt gtgggcgcgc atcatttcca
      721 cttcgtgcat aaagccgaaa gtgcgcgcgc gcgcgatttc gtcgatgtag gatttgccgg
      781 cgaaatcaat ttcaaaagtg ggcgagctgc ggttgaaaac cggatggtcg aattcgatgg
      841 tcagcgttac cttaaagccg tcatacggcg taaagcgcac ccatttgccc gcttctttga
      901 tttcgacagg cttgaggatt ttcaaaaaac gcttttgcgc cttttgatcg accacgcccg
      961 catcttgcaa aaggtaaata aacggcaggc tggagccgtc cataatcggg atttcgggcg
     1021 cgttcagctc aatcagtgcg ttgtcgatac cgtaggcgga cagcgcggac ataatgtgtt
     1081 cgatcgtgcc gacgcgcacg cctttgtcgg taacgatggt ggatgaaagg cgggtatcgt
     1141 tgatcaaata aggggtcagc ttgatttgtt cgcccatctc gccgtccaaa tcggtgcgtc
     1201 ggaaggaaat cccgctgttt tcaggcgcgg ggtgcagggt cagtgcgacg cgttcgcccg
     1261 aatgcagccc gactccggta acgctgatgg atttcgccaa agttctttgc agcataaacc
     1321 gcttccttat caatggggta agttggggaa tgatacgata aaaccggaaa aacaagctat
     1381 gtttttccat agtatttgcc aatgtatccg ttttcaatac gtaagccgca taaaaatgta
     1441 tagtggatta acaaaaatca agacaaggcg acgaagccgc ccccccctcc tgaaaaacgc
     1501 aaaaaatgcc gtccgaaaac ctttcggacg gcattttcgc gtaaaccgtc attccctcaa
     1561 aaacagaaaa ccaaaaacag aaacctaaaa tttcatcatt ccctcaaaaa cagaaaaccc
     1621 aaaacagaaa cctaaaattc gtcattcccg caaaagcggg aatctagttc gttgagtttc
     1681 agttatttag aataaatttt gaaacttaaa tcccgtcatt cccacgaaag tgggaatcca
     1741 gaacgcaaaa tctaaagaaa ccgttttccc gataagtttc cgtaccgaca aggctggatt
     1801 cccgcctgcg cgggaatgac gaagctatcc ttttggtcga aggtcaaaaa tcagccgtca
     1861 cagagtatta cctgaatcac ggcaaatggc ccggcggcaa cagtgatgcc ggcgtggcat
     1921 cctcctctaa aatcaaagga aagagacctt tgcaaaattc ctttccctcc cgacagccga
     1981 aacccaaaca caggttttcg gctgttttca gctgttttcg ccccaaatac ccccttaatc
     2041 ctccacggat acccgataat caggcatccg gtc
//
LOCUS       DAWHSK010000283         2059 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.283, whole genome shotgun sequence.
ACCESSION   DAWHSK010000283 DAWHSK010000000
VERSION     DAWHSK010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.283"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..661
                     /locus_tag="WIC03_001983"
     CDS             <1..661
                     /locus_tag="WIC03_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HEZ3884435.1"
                     /translation="VIRWSIDGWQPKSEPGAMAARNLLGFRDGTGNPKVSDPKTADEV
                     LWTGVAANSLDEPEWAKNGSYQAVRLIRHFVEFWDRTPLQEQTDIFGRRKYSGAPMDG
                     KKEADQPDFAKDPEGDITPKDSHIRLANPRDPEFLKKHRLFRRAYSYSRGLASSGQLD
                     VGLVFVCYQANLADGFIFVQNLLNGEPLEEYISPFGGGYFFVLPGVEKGGFLGQGLLG
                     V"
     gene            complement(730..1062)
                     /locus_tag="WIC03_001984"
     CDS             complement(730..1062)
                     /locus_tag="WIC03_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEZ3884436.1"
                     /translation="MSLPPCPQCASEYTYEDGGQYICPECAHEWNKTESAAAEAAEEV
                     RDANGAVLQNGDTIILIKDLKVKGSSMTIKQGTKVKGIRLQEGDHNIACKIDGSAMNL
                     KSEFVKKA"
     gene            complement(1137..>2059)
                     /gene="glmU"
                     /locus_tag="WIC03_001985"
     CDS             complement(1137..>2059)
                     /gene="glmU"
                     /locus_tag="WIC03_001985"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEZ3884437.1"
                     /translation="IVEEKDADTAQKAVKETNTGILVLPNAKLENWLNSLSSNNAQGE
                     YYLTDLIAKAVADGIKVHPVQVRASHLAAGVNNKRQLAELERIFQTGQAQELLKAGVT
                     LHDPARFDLRGRLKHGQDVVIDVNCIFEGEVEIGDNVEIGANCVIKNAKIGANSKIAP
                     FSHLEDCEVGENNRIGPYARLRPQARLADDVHVGNFVEIKNAAIGKGTKANHLTYIGD
                     AEVGSKTNFGAGTIIANYDGVHKHKTVIGDEVRIGSNCVLVAPVTLGNKVTTGAGSTI
                     TRNVEDNKLALARARQTVIEGWVRPEKDKQ"
BASE COUNT          364 a          519 c          640 g          536 t
ORIGIN      
        1 cgttatccgt tggagtatcg acgggtggca gcccaaatcc gaacccggcg cgatggcggc
       61 gcgcaacctg ttgggcttca gggacggcac gggcaacccc aaagtttccg accccaaaac
      121 tgccgacgag gttttgtgga cgggggtggc cgccaacagc ctcgacgaac cggagtgggc
      181 gaaaaacggc agctatcagg cagtccgcct tatccgccac tttgtcgagt tttgggacag
      241 gacgccgctt caagagcaaa ccgacatttt cgggcggcgc aaatacagcg gtgcgccgat
      301 ggacggcaaa aaagaagccg accaaccgga ttttgccaaa gaccccgagg gtgatatcac
      361 gcccaaagac agccatatac gcctggcgaa tccgcgcgat cccgaattcc tcaaaaaaca
      421 ccgcctcttc cgccgcgcct acagctattc gcgcggactc gcctcaagcg gacagcttga
      481 tgtcgggctg gtgttcgtct gctatcaggc aaaccttgcc gacggattca tcttcgtgca
      541 aaacctcctc aacggcgaac cgctggaaga atacatcagc cccttcggcg gcggctattt
      601 cttcgtcttg cccggcgtgg aaaaaggcgg ctttttgggg caagggctgc tgggcgtata
      661 aatccgccat ataaaaaacg ccgtctgaat ccgataaggg gttcagacgg cattttactg
      721 tttgggcggt caggcttttt tgacgaattc agattttaaa ttcatcgcgc tgccgtcgat
      781 tttgcaggcg atgttgtgat cgccttcctg caggcgtatg cctttgactt tcgtaccttg
      841 tttgatcgtc atcgagctgc cttttacctt gaggtctttg atgaggatga tggtgtcgcc
      901 gttttgcagc accgcgccgt tggcatcgcg cacttcttcg gcggcttcgg ctgcggcgga
      961 ttcggtttta ttccattcgt gggcgcattc ggggcagatg tattgtccgc cgtcttcgta
     1021 ggtgtattcg gaggcgcatt gcgggcaagg gggtaatgac atggtttgcc gtccttatcg
     1081 gatgtttgtt ttggggtgcc gtctgaaacc tgaaaccggc ttcagacggc atagctttat
     1141 tgtttgtctt tttcaggacg cacccagcct tcgatgacgg tttggcgggc gcgggcgagg
     1201 gcgagtttgt tgtcttcgac attgcgggta atcgtgctgc ccgcgcctgt ggttactttg
     1261 ttgccgaggg taacgggggc gactaggacg cagtttgaac cgatgcgcac ttcgtcgccg
     1321 atgacggttt tgtgtttgtg cacgccgtcg tagttggcaa taatcgtacc ggcgccgaag
     1381 ttggttttgc tgccgacttc ggcatcgccg atgtaggtaa ggtggttggc tttggtgcct
     1441 ttgccgatgg cggcgttttt gatttcgacg aagttgccga cgtgtacgtc gtctgcaagg
     1501 cgggcttgcg gacgcaggcg ggcgtacggg ccgattcggt tgttttcgcc gacttcgcag
     1561 tcttcgaggt gggagaaggg ggcgattttg ctgtttgcac cgattttggc gtttttgatg
     1621 acgcagtttg cgccgatttc gacgttgtcg ccgatttcga cttcgccttc aaagatacag
     1681 ttcacatcaa ttacgacatc ctgtccgtgt ttcagacggc ctcgtaaatc gaaacgtgcc
     1741 gggtcgtgca gggtaacgcc tgctttgagc aattcttgcg cctgtccggt ttggaagatg
     1801 cgttcgagtt cggcgagctg gcgtttgttg ttcacgccgg cggcgaggtg ggaggcgcgg
     1861 acttggacgg gatgaacttt aataccgtcg gcaacggctt tggcgatgag gtcggtcagg
     1921 tagtattcgc cttgggcatt gttgctggaa aggctgttca gccagttttc cagtttggcg
     1981 ttgggcagga cgaggatgcc ggtgttggtt tctttcacgg ctttttgggc ggtatcggcg
     2041 tctttttctt cgacaatgg
//
LOCUS       DAWHSK010000284         2054 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.284, whole genome shotgun sequence.
ACCESSION   DAWHSK010000284 DAWHSK010000000
VERSION     DAWHSK010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.284"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            191..1987
                     /locus_tag="WIC03_001986"
     CDS             191..1987
                     /locus_tag="WIC03_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA family autotransporter adhesin"
                     /protein_id="HEZ3884438.1"
                     /translation="MNKIYRIIWNSALNAWVVVSELTRNHTKRASATVATAVLATLLF
                     ATVQANATDDDDLYLEPVQRTAVVLSFRSDKEGTGEKEGTEDSNWAVYFDEKRVLKAG
                     AITLKAGDNLKIKQNTNENTNENTNDSSFTYSLKKDLTDLTSVETEKLSFGANGNKVN
                     ITSDTKGLNFAKETAGTNGDPTVHLNGIGSTLTDTLLNTGATTNVTNDNVTDDEKKRA
                     ASVKDVLNAGWNIKGVKPGTTASDNVDFVRTYDTVEFLSADTKTTTVNVESKDNGKKT
                     EVKIGAKTSVIKEKDGKLVTGKGKDENGSSTDEGEGLVTAKEVIDAVNKAGWRMKTTT
                     ANGQTGQADKFETVTSGTNVTFASGKGTTATVSKDDQGNITVKYDVNVGDALNVNQLQ
                     NSGWNLDSKAVAGSSGKVISGNVSPSKGKMDETVNINAGNNIEITRNGKNIDIATSMA
                     PQFSSVSLGAGADAPTLSVDDEGALNVGSKDTNKPVRITNVAPGVKEGDVTNVAQLKG
                     VAQNLNNRIDNVDGNARAGIAQAIATAGLVQAYLPGKSMMAIGGGTYRGEAGYAIGYS
                     SISDGGNWIIKGTASGNSRGHFGASASVGYQW"
BASE COUNT          634 a          496 c          517 g          407 t
ORIGIN      
        1 cggaaactta ccgaataaaa cagtttcctt agattccacg tcccagattc ccgccttcgc
       61 gggaatgacg agattttaag ttgggggaat ttatcagaaa acccccaacc cccaaaaacc
      121 gggcggatgc cgcaccatcc gcccccaaac cccgatttaa ccattcaaac aaaccaaaag
      181 aaaaaacaaa atgaacaaaa tataccgcat catttggaat agtgccctca atgcctgggt
      241 cgtcgtatcc gagctcacac gcaaccacac caaacgcgcc tccgcaaccg tggcgaccgc
      301 cgtattggcg acactgttgt ttgcaacggt tcaggcgaat gctaccgatg acgacgattt
      361 atatttagaa cccgtacaac gcactgctgt cgtgttgagc ttccgttccg ataaagaagg
      421 cacgggagaa aaagaaggta cagaagattc aaattgggca gtatatttcg acgagaaaag
      481 agtactaaaa gccggagcaa tcaccctcaa agccggcgac aacctgaaaa tcaaacaaaa
      541 caccaatgaa aacaccaatg aaaacaccaa tgacagtagc ttcacctact ccctgaaaaa
      601 agacctcaca gatctgacca gtgttgaaac tgaaaaatta tcgtttggcg caaacggtaa
      661 taaagtcaac atcacaagcg acaccaaagg cttgaatttt gcgaaagaaa cggctgggac
      721 gaacggcgac cccacggttc atctgaacgg tatcggttcg actttgaccg atacgctgct
      781 gaataccgga gcgaccacaa acgtaaccaa cgacaacgtt accgatgacg agaaaaaacg
      841 tgcggcaagc gttaaagacg tattaaacgc aggctggaac attaaaggcg ttaaacccgg
      901 tacaacagct tccgataacg ttgatttcgt ccgcacttac gacacagtcg agttcttgag
      961 cgcagatacg aaaacaacga ctgttaatgt ggaaagcaaa gacaacggca agaaaaccga
     1021 agttaaaatc ggtgcgaaga cttctgttat taaagaaaaa gacggtaagt tggttactgg
     1081 taaaggcaaa gacgagaatg gttcttctac agacgaaggc gaaggcttag tgactgcaaa
     1141 agaagtgatt gatgcagtaa acaaggctgg ttggagaatg aaaacaacaa ccgctaatgg
     1201 tcaaacaggt caagctgaca agtttgaaac cgttacatca ggcacaaatg taacctttgc
     1261 tagtggtaaa ggtacaactg cgactgtaag taaagatgat caaggcaaca tcactgttaa
     1321 gtatgatgta aatgtcggcg atgccctaaa cgtcaatcag ctgcaaaaca gcggttggaa
     1381 tttggattcc aaagcggttg caggttcttc gggcaaagtc atcagcggca atgtttcgcc
     1441 gagcaaggga aagatggatg aaaccgtcaa cattaatgcc ggcaacaaca tcgagattac
     1501 ccgcaacggt aaaaatatcg acatcgccac ttcgatggcg ccgcagtttt ccagcgtttc
     1561 gctcggtgcg ggggcggatg cgcccacttt gagcgtggat gacgagggcg cgttgaatgt
     1621 cggcagcaag gataccaaca aacccgtccg cattaccaat gtcgccccgg gcgttaaaga
     1681 gggggatgtt acaaacgtcg cacaacttaa aggcgtggcg caaaacttga acaaccgcat
     1741 cgacaatgtg gacggcaacg cgcgtgcggg catcgcccaa gcgattgcaa ccgcaggtct
     1801 agttcaggcg tatctgcccg gcaagagtat gatggcgatc ggcggcggca cttatcgcgg
     1861 cgaagccggt tacgccatcg gctactccag tatttccgac ggcggaaatt ggattatcaa
     1921 aggcacggct tccggcaatt cgcgcggcca tttcggtgct tccgcatctg tcggttatca
     1981 gtggtaaggg ctttttcgcc tgtctgctgt tggggcaggc ggaaggtttg aagggaaggg
     2041 tggcgatttg ccgc
//
LOCUS       DAWHSK010000285         2034 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.285, whole genome shotgun sequence.
ACCESSION   DAWHSK010000285 DAWHSK010000000
VERSION     DAWHSK010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.285"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..172)
                     /locus_tag="WIC03_001987"
     CDS             complement(<1..172)
                     /locus_tag="WIC03_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ3884439.1"
                     /translation="MMLKKFVLGGIAALVLAACGGSEGGSGASSAPAQSAVSGSLIER
                     INNKGTVTVGTEG"
     gene            457..1365
                     /locus_tag="WIC03_001988"
     CDS             457..1365
                     /locus_tag="WIC03_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HEZ3884440.1"
                     /translation="MNTPQSAIIPDHAQAGIFIEADFAANRLNDIKSACRASLDALSA
                     LKTRFPDDILGLTIAFGSKAWATFGHTDEGSEIKPFSEMGNGLAPSTQHDMSIHIQSF
                     RQNAAYALAQSVLGAFGDSICVASEEHGLRLYQDRGLDGFVDGTENPQGDENVREVAI
                     IPEGKPDAGGSYVLLQKYLHDLKKWDAVPVAEQEASVGRGKETDDEFGHDVRLPDSHL
                     GRVNLKENGVGLKIVRRSLPFGKISGEHGLMFTAYCRTLHNIEAQLLSMFGDTDGKTD
                     LLLRHLSAAVSGGYYYAPSVERLQNL"
     gene            1445..>2034
                     /locus_tag="WIC03_001989"
     CDS             1445..>2034
                     /locus_tag="WIC03_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEZ3884441.1"
                     /translation="MSAPIQAFDPLTVPISGTNLIEASAGTGKTYGIAALFTRLIVLE
                     QKNVERVLVVTFTKAATAELKTRLRARLDDVLQVLESKEIAKLGDDTLSDGIAAYCAE
                     HHKGDTFLPALLKQALQKEGRTRLIVRLKAAIGQFDNAAIYTIHGFCQRILRDYAFLC
                     QAPFDVELTEEDGDRLLIPAQDFWRERVSNDPVLAA"
BASE COUNT          492 a          638 c          509 g          395 t
ORIGIN      
        1 tgccttccgt gccgacggta accgtgcctt tattgttgat gcgctcgatt aaagaaccgg
       61 aaactgccga ttgtgcaggc gcggaagatg ctccgctgcc gccttccgaa ccgccgcagg
      121 ccgccaaaac caatgcggca ataccgccga gtacgaattt tttcaacatc attctctcct
      181 gtaaaaagtg tgcaagtgcg gcattctaag tgaaattgcg gcaaacacaa agaatacttt
      241 ttttatatgg ttattccaaa tcggaatgga aaagatatgc cgaagccgcc ggaagcggaa
      301 ataaaggggg cgggaaaggc ggcgggcggc acgaaaccga aaagcggcat caggtatgca
      361 aacggcggat tcacgcacgc cgttccggca aaaatccgcc tgccgattcg gtgctataat
      421 ccatccgaaa cagatgacac caaaaaggaa catctcatga acacgcccca atccgccatc
      481 attcccgacc acgcccaagc cggcatcttt atcgaagccg acttcgccgc caaccgcctc
      541 aacgatatta aatccgcctg ccgcgcttcg ctcgacgcgt tatccgcctt gaaaacccgc
      601 tttccggacg atattttggg tctgaccatc gccttcggca gcaaagcctg ggcaacattc
      661 ggacatacgg acgaaggcag tgaaatcaaa cccttctccg aaatgggcaa cgggcttgcg
      721 ccgtctacgc agcacgatat gtccatccac atccaatcct tccgccaaaa cgccgcctac
      781 gcgcttgccc aatccgtttt gggcgcgttc ggcgacagca tatgcgtcgc gtccgaagaa
      841 cacggtttgc gcctgtatca ggatcgcggg ctggacggtt tcgtcgacgg cacggaaaac
      901 ccgcagggcg atgaaaatgt ccgcgaagtc gccatcatcc ccgaaggaaa acccgatgcg
      961 ggcggcagct atgtcctgct gcaaaaatac ctgcacgatt tgaaaaaatg ggatgccgtc
     1021 cccgtcgccg aacaggaggc ctcggtcgga cgcggcaagg aaaccgacga cgaattcggc
     1081 cacgatgtcc gcctgcccga ttcgcacctc ggccgcgtca acctgaaaga aaacggagtc
     1141 ggcctgaaaa tcgtccgccg cagcctgccc ttcggcaaaa tcagcggcga acacggtttg
     1201 atgtttaccg catactgccg cacgctgcac aatatcgaag cgcaattatt gagtatgttc
     1261 ggcgacacgg atggcaaaac cgacctgctg ctccggcacc tctccgccgc cgtttcgggc
     1321 ggatactact acgcgccgtc tgtcgaacgc ctgcaaaacc tctaagccag acgatgccgt
     1381 ctgaaaccct ttttcagacg gcattttccc gttttaccgt ccaaccgttt tccgagcgcg
     1441 cactatgtct gcacccatcc aagcatttga cccgctgacc gtccccattt ccggcaccaa
     1501 cctgattgag gcctctgccg gcaccggcaa aacctacggc atcgccgccc tgtttacgcg
     1561 cctgatcgta ttggaacaaa aaaacgtcga gcgcgtattg gtcgttacct tcaccaaagc
     1621 cgccaccgcc gagttgaaaa cacgcctgcg cgcacgtttg gacgatgtgt tgcaggtttt
     1681 agaaagtaaa gaaattgcca aacttggaga cgacacgctt tcagacggca tcgccgccta
     1741 ctgcgccgaa caccacaaag gcgacacctt cctgcccgca ctcttaaaac aggctttgca
     1801 aaaagagggc cggacgcgtc tgattgtccg cctcaaggcc gccatcgggc aattcgacaa
     1861 cgccgccatc tataccatcc acggcttctg ccaacgtatc ctgcgcgact acgccttctt
     1921 gtgccaagca ccgttcgatg tcgaactgac cgaagaagac ggcgaccgcc tgcttatccc
     1981 ggcgcaagat ttttggcggg aacgcgtcag caatgatccg gtgcttgccg catt
//
LOCUS       DAWHSK010000286         2025 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.286, whole genome shotgun sequence.
ACCESSION   DAWHSK010000286 DAWHSK010000000
VERSION     DAWHSK010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.286"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            220..768
                     /locus_tag="WIC03_001990"
     CDS             220..768
                     /locus_tag="WIC03_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEZ3884442.1"
                     /translation="MARLTVHTLETAPEAAKARVEAVLQNNGFIPNLIGVLSNAPEAL
                     AFYQEVGKLNAANSLTAGEVEVIQIIAARTNQCGFCVAGHTKLATLKKLLSEQSVKAA
                     RALAAGEFDDAKLGALAAFTQAVMAKKGAVSDDELKAFFDAGYNQQQAVEVVMGVALA
                     TLCNYVNNLAQTEINPELQAYA"
     gene            903..1994
                     /locus_tag="WIC03_001991"
     CDS             903..1994
                     /locus_tag="WIC03_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HEZ3884443.1"
                     /translation="MNAQTLIANVAELVKTKLKPIVDDIDRKGYYPEAFMRELGAIGG
                     FGAVGTEAEGGNGLGLATQIAVLREIGKECGATSFSAWCQAACAWYLHQTPNQAVKDK
                     YLADILQGRVLAGTGMSNTVKHLAGIEKHNLQAERADGGYTVNGALPWVSNIGEDHIW
                     ANTAQIGGGYVMFITGGQWEGVSLQNCPEFCALEGTRTFSLNFKDVFIPDEDIIAAPE
                     QFADYIQSIKAGFILLQIGIGAGVIDGSLGIIRLANIVNAEVNRYLDDGYDSLKARLD
                     GAWAETERLAGLAWNNTPDTLATLKLREAAAVLALAAAQSAALHSGAKGYLMRSPAQR
                     RVREAMFVAIVTPAIKHLRKEIAAIEAAK"
BASE COUNT          538 a          658 c          494 g          335 t
ORIGIN      
        1 aaacggttga ggcagccaat ccccaagccg cattggcgca atatttcggg cgcgggggcc
       61 gataagcgcg ttttcttttc catcatgccg tctgaaaaat gttcagacgg catttcttat
      121 aaccttttgt ttgcagcaca taaccaatca gtctttccta ttccgttccg aaatgcatac
      181 catgcgcccg tacccaatat ttgcgaacaa ggaaagaaaa tggcacgttt aaccgtacac
      241 accctcgaaa ccgcccccga agccgccaaa gcgcgcgtcg aggcggtact tcaaaacaac
      301 ggctttatcc ccaaccttat cggcgtatta tcaaacgccc ccgaagcctt ggcgttttac
      361 caagaagtcg gcaagctcaa cgccgccaac agcctgaccg ctggcgaagt cgaagtcatc
      421 cagatcatcg ccgcccgtac caaccaatgc ggtttttgcg tggcagggca caccaaactc
      481 gcaaccctga aaaaactcct gtccgaacaa tccgtcaaag ccgcgcgcgc tttggcagca
      541 ggcgaatttg acgatgccaa actcggcgcg ctcgccgcct tcacccaagc cgtaatggcg
      601 aaaaaaggcg cggtatccga tgacgaactc aaagcatttt tcgacgcggg ctacaaccag
      661 cagcaggcag ttgaagtcgt gatgggcgta gccttggcaa ccctgtgcaa ctacgtcaac
      721 aacctcgccc aaaccgaaat caaccccgaa ttgcaggctt acgcctgata cggcaaaccg
      781 cccgaatatc gggcggtttt tcaacccccc ctcctcaaac aaaaacaagc cgccaagcgg
      841 cagggaaagc agccgcgcat caggcgcgcc cccgattccc ccgcaaacct taaggaacaa
      901 agatgaacgc ccaaaccctg attgccaacg ttgccgaact cgtcaaaacc aagctcaaac
      961 ccatagtgga cgacatcgac cgcaaaggat actaccccga agcatttatg cgcgaactcg
     1021 gcgcaatcgg cggattcggc gcagtcggga cagaagccga aggcggcaac ggcttaggct
     1081 tggcaacgca aatcgccgtt ttgcgcgaaa tcggcaaaga atgcggcgca acctccttca
     1141 gcgcgtggtg tcaggcggct tgcgcgtggt atctgcacca aacgcccaac caagccgtca
     1201 aagacaaata cctcgccgac atcctgcaag gcagagtatt ggcaggtacc ggaatgtcca
     1261 ataccgtcaa acaccttgcc ggcatcgaaa aacacaacct tcaagccgag cgcgcagacg
     1321 gcggctacac agtcaacggc gcgctgccgt gggtgtccaa catcggcgaa gaccacatct
     1381 gggcaaatac cgcccaaatc ggcggcggct acgtcatgtt tatcaccggc ggacaatggg
     1441 aaggcgtaag cctgcaaaac tgccccgaat tttgcgccct cgaaggtacg cgcaccttca
     1501 gcctgaactt caaagacgta tttatccccg acgaagacat catcgccgcg cccgaacagt
     1561 ttgccgacta catccaaagc atcaaagcag gtttcatcct cctgcaaatc ggcatcggcg
     1621 cgggcgtgat tgacggcagc ctcggcatca tccgcctcgc caacatcgtc aacgccgaag
     1681 tcaaccgcta tctcgacgac ggctacgaca gcctcaaagc aaggttggac ggcgcatggg
     1741 cagaaaccga acggctcgcc ggtttggcat ggaacaacac gccggacaca ttagcgaccc
     1801 tcaagctgcg cgaagccgcc gccgtactgg cacttgccgc cgcccaatcc gccgccctgc
     1861 attccggcgc aaaaggctac ctgatgcgca gtcccgccca aaggcgcgtc cgcgaagcaa
     1921 tgtttgtcgc catcgtaacc cccgcgatca aacacctccg aaaagaaata gcggcaatcg
     1981 aagccgcaaa ataaacaggc ggaaaaaacc gaaaccccca ccgcg
//
LOCUS       DAWHSK010000287         2018 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.287, whole genome shotgun sequence.
ACCESSION   DAWHSK010000287 DAWHSK010000000
VERSION     DAWHSK010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.287"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..1858
                     /gene="infB"
                     /locus_tag="WIC03_001992"
     CDS             <1..1858
                     /gene="infB"
                     /locus_tag="WIC03_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEZ3884444.1"
                     /translation="ARNGDDERVRGGKKGKKLKLEPNQHAFQAPTEPVVHEVLVPETI
                     TVADLAHKMAVKGVEVVKALMKMGMMVTINQSIDQDTALIVVEELGHIGKPAAADDPE
                     AFLDEGAEAVEAEALPRPPVVTVMGHVDHGKTSLLDYIRRAKVVQGEAGGITQHIGAY
                     HVETPRGVITFLDTPGHEAFTAMRARGAKATDIVILVVAADDGVMPQTIEAIAHAKAA
                     GVPIVVAVNKIDKEAANPERIRQELTAHEVVPDEWGGDVQFIDVSAKKGLNIDALLEA
                     VLLEAEVLELTAPVDAPAKGIIVEARLDKGRGAVATLLVQSGTLKKGDMLLAGTAFGK
                     IRAMVDENGKSITEAGPSIPVEILGLSDVPNAGEDAMVLADEKKAREIALFRQGKYRD
                     VRLAKQQAAKLENMFNNMGETQAQSLSVIIKADVQGSYEALAGSLKKLSTDEVKVNVL
                     HSGVGGITESDVNLAIASGAFIIGFNVRADASSRKLAENENVEIRYYNIIYDAIDDVK
                     AAMSGMLSPEEKEQVTGTVEIRQVISVSKVGNIAGCMVTDGVVKRDSHVRLIRNNVVI
                     HTGELASLKRYKDDVKEVRMGFECGLMLKGYNEIMEGDQLECFDIVEVARSL"
BASE COUNT          520 a          571 c          544 g          383 t
ORIGIN      
        1 tgcacgcaat ggcgacgacg agcgcgtacg cggcggcaaa aaaggcaaaa aactcaaact
       61 cgagccgaac caacacgcct tccaagcacc gaccgaacct gtcgtacacg aagttttggt
      121 gcctgaaacc attaccgttg ccgacttggc acacaaaatg gcagtcaaag gcgtggaagt
      181 ggtcaaagcc ctgatgaaga tgggcatgat ggttaccatc aaccaatcca tcgaccaaga
      241 caccgccctg attgtcgtcg aagaactcgg acacatcggc aaacccgccg ccgccgacga
      301 tcccgaagcc ttcttggacg agggcgcgga agcggtggaa gccgaagcat tgccgcgtcc
      361 gcccgtcgtt accgtcatgg gacacgtcga ccacggcaaa acctcgctgc tggactacat
      421 ccgccgtgcc aaagtggtac agggcgaagc gggcggcatt acccaacaca tcggtgcata
      481 ccacgttgaa accccacgcg gcgtgattac cttcttggat actccgggtc acgaagcgtt
      541 taccgccatg cgcgcgcgcg gcgcgaaagc gaccgacatc gtgattctcg tggttgccgc
      601 agacgacggt gtaatgccgc aaaccatcga agccattgca cacgccaaag cggcaggcgt
      661 tccgattgtg gttgccgtca acaaaatcga taaagaagcc gccaacccag agcgtatccg
      721 ccaagagctg accgcacacg aagtcgtgcc tgacgaatgg ggcggcgatg tacagtttat
      781 cgacgtttcc gctaaaaaag gcctgaacat cgatgcattg ctcgaggcag tcttgctcga
      841 agccgaagtt ttggaactga ccgcacctgt cgatgcgccc gccaaaggca tcatcgtcga
      901 ggcgcgcttg gacaaaggcc gcggcgcggt tgccacattg ctggttcaaa gcggcacgct
      961 gaaaaaaggc gatatgctgc tggccggtac ggcattcggc aaaatccgcg cgatggtcga
     1021 tgaaaacggc aaatccatta ccgaagccgg tccgtccatc cccgtcgaaa tcctcggctt
     1081 gtccgacgtt ccgaacgcag gcgaagatgc aatggtattg gctgatgaga aaaaagcgcg
     1141 cgaaatcgcc ctcttccgtc aaggcaaata ccgcgatgtg cgccttgcca aacaacaggc
     1201 ggcgaagctg gaaaatatgt tcaacaatat gggcgaaacc caggcccaat ctttgtcggt
     1261 catcatcaag gcagacgtgc agggctctta cgaggctttg gcgggcagcc tgaaaaaact
     1321 gtccacagac gaagtgaaag tgaacgtgtt gcacagcggc gtgggcggca ttaccgaatc
     1381 tgatgtcaac cttgccatcg cttcgggcgc attcattatc ggctttaacg tgcgtgcaga
     1441 tgcctcttcg cgcaaacttg ccgaaaatga aaacgtggaa atccgctact acaacatcat
     1501 ctacgatgcc atcgacgacg tgaaggcggc gatgagcggt atgctttccc cggaagagaa
     1561 agaacaggtt accggtacgg tcgaaatccg tcaggtcatc tccgtttcca aagtcggcaa
     1621 cattgcaggc tgtatggtta ccgacggcgt ggtcaaacgc gattcccatg tccgcctcat
     1681 ccgcaacaac gtggttatcc acacgggcga actggcttcg ttgaaacgct ataaagacga
     1741 tgtcaaagaa gtccgcatgg gcttcgagtg cggtctgatg ctcaaaggct acaacgaaat
     1801 catggaaggc gaccaactgg aatgcttcga catcgtcgaa gttgcccgca gcctgtaatt
     1861 cctttgcaaa taaaatgccg tctgaagcgt tcagacggca acgaaacggg ttctgtatca
     1921 tacagaaccc gttttttgtc gcaaatcggc ttcagacagc cctcttgcct tatcccgatt
     1981 tgaatctgac ttgccataca aacaggcttc agacggca
//
LOCUS       DAWHSK010000288         1998 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.288, whole genome shotgun sequence.
ACCESSION   DAWHSK010000288 DAWHSK010000000
VERSION     DAWHSK010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.288"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(90..1037)
                     /locus_tag="WIC03_001993"
     CDS             complement(90..1037)
                     /locus_tag="WIC03_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraslipin"
                     /protein_id="HEZ3884445.1"
                     /translation="MEFFIILLVAVAVFGFKSFVVIPQQEVHVVERLGRFHRALTAGL
                     NILIPFIDRVAYRHSLKEIPLDVPSQVCITRDNTQLTVDGIIYFQVTDPKLASYGSSN
                     YIMAITQLAQTTLRSVIGRMELDKTFEERDEINSTVVSALDEAAGAWGVKVLRYEIKD
                     LVPPQEILRSMQAQITAEREKRARIAESEGRKIEQINLASGQREAEIQQSEGEAQAAV
                     NASNAEKIARINRAKGEAESLRLVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVA
                     AFNNLAKESNTLIMPANVADIGSLISAGMKIIDSSKTAK"
     gene            complement(1052..1459)
                     /locus_tag="WIC03_001994"
     CDS             complement(1052..1459)
                     /locus_tag="WIC03_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEZ3884446.1"
                     /translation="MTVWFVAAVAVLIIELLTGTVYLLVVSAALAGSGIAYGLTGSTP
                     AAVLTAALLSALGIWFVHAKTAVGKVETDSYQDLDAGQYAEILRHAGGNRYEVFYRGT
                     HWQAQNTGQDVFEPGTRALIVRKEGNLLIITHP"
     gene            1540..>1998
                     /gene="ung"
                     /locus_tag="WIC03_001995"
     CDS             1540..>1998
                     /gene="ung"
                     /locus_tag="WIC03_001995"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEZ3884447.1"
                     /translation="MDTWHDALGNEKRQPYFQEILNAVRQERLSGQIIYPPAEDVFNA
                     FRLTAFDRVKVVILGQDPYHGAGQAHGLAFSVRQGVRIPPSLLNIYKELETDINGFSI
                     PDHGCLTAWAEQGVLLLNTVLTVRAGQAHSHALLGWERFTDTVIRQLATHR"
BASE COUNT          468 a          508 c          551 g          471 t
ORIGIN      
        1 ttgaagcgga atacaacctt gcccgggttt aaataaaaat gccgtctgaa ccccgaaaac
       61 agagcttcag acggcatttt catcacggct tatttggcgg ttttgctgct gtcgataatt
      121 ttcataccgg cagaaatcag gctgccgatg tcggcaacat tggcgggcat aatcagcgta
      181 ttgctttctt tggcaagatt gttgaacgca gcgacgtatt gttccgcaat cttcagattg
      241 accgcatccg cgccgccttg ggtttgaagg gcggcggcaa tttgacggat ggcttcggca
      301 ttggcttcgg caacaaggcg cagggattcc gcttcgcctt tggcgcggtt gatgcgggcg
      361 attttctcgg catttgacgc attgaccgcg gcctgggcct cgccttcgga ttgttggatt
      421 tcggcttcgc gctgaccact ggcaaggttg atttgttcga ttttacggcc ttcggattcg
      481 gcgatacggg cgcgtttttc gcgttcggca gtaatttgcg cctgcattga gcgaaggatt
      541 tcttgcggcg gaaccaaatc cttgatttcg taacggagaa ctttcacacc ccaagctccg
      601 gcggcttcat cgagggcgga aacgacggtg ctgttgattt cgtcgcgttc ttcaaacgtt
      661 ttgtccaact ccatacgccc gataacggaa cgcagcgtcg tttgggcaag ctgggtaatc
      721 gccataatgt agttgctcga accgtatgag gcgagtttgg gatcggttac ttggaaatag
      781 atgatgccgt caaccgtcag ctgggtgttg tcgcgcgtga tgcagacctg gctgggtacg
      841 tctaaaggga tttctttcag cgaatgacgg taggcgacgc ggtcgataaa gggaatcaaa
      901 atattcaaac cggccgtcaa ggcgcgatgg aaacgtccaa gcctttcgac aacgtggact
      961 tcctgttgtg ggatgacaac aaaggatttg aaaccgaaaa cggcgacggc taccaacaag
     1021 ataatgaaaa attccataat tcctccgagt gttaagggtg tgtgataata agaaggttgc
     1081 cttccttgcg gacaatgagg gcgcgcgttc ccggttcaaa cacatcctgc cccgtatttt
     1141 gcgcctgcca gtgcgtaccg cgataaaaaa cttcgtaacg gttgccgcct gcgtgccgga
     1201 ggatttcggc atattgcccg gcatccaaat cctgatatga atccgtttca acttttccca
     1261 cggcggtctt ggcgtgtacg aaccaaatac ccagcgcgga aagcagagcg gcggtcaaga
     1321 cggcggcagg cgtgctgccg gtcagcccgt aagcaatgcc cgaacccgcc aaagccgcgc
     1381 tgacaaccaa aagataaacc gttcccgtca ataattcgat gattaagacg gcaacagcgg
     1441 caacaaacca tacagtcata catttcccca caaagcgcgt cgtttgacaa aatcacgcga
     1501 tatcagcagt atagccgaat ttgaaaggat agggcagata tggatacttg gcacgatgca
     1561 ctgggcaacg aaaaacggca accgtatttt caggaaattt taaatgcagt caggcaggaa
     1621 cgtttgtcgg ggcaaatcat ctatccgccg gcggaggatg tgttcaacgc attccgcctg
     1681 acagcgtttg accgagtcaa agtcgtcatt ctcggacaag acccgtatca cggggcaggg
     1741 caggcgcacg gtttggcatt ttccgtcagg cagggtgtcc gcataccgcc gtctttactc
     1801 aatatctaca aagagttgga aaccgacatc aacggttttt ccattcccga tcacggctgc
     1861 ctgacggctt gggcggagca gggcgtattg cttctgaaca cggttttgac ggtgcgtgca
     1921 ggacaggcgc attcgcacgc ccttttaggc tgggaacgct ttaccgatac cgtcatcagg
     1981 cagcttgcga cacaccgc
//
LOCUS       DAWHSK010000289         1997 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.289, whole genome shotgun sequence.
ACCESSION   DAWHSK010000289 DAWHSK010000000
VERSION     DAWHSK010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.289"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            113..1504
                     /locus_tag="WIC03_001996"
     CDS             113..1504
                     /locus_tag="WIC03_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEZ3884448.1"
                     /translation="MSGQLGKGADAPDLVYGLEDRPPFGNALLSAVTHLLAIFVPMIT
                     PALIVGGALELPVEMTAYLVSMAMVASGVGTYLQVNRFGPVGSGMLSIQSVNFSFVTV
                     MIALGAGMKEGGLTKDAMISTLLGVSFVGAFLVCFSAWLLPYLKKVITPTVSGVVVML
                     IGLSLVHVGITDFGGGFGAKADGTFGSMENLGLASLVLLIVLIFNCMKNPLLRMSGIA
                     VGLIAGYIVALFLGKVDFSALQNLPLVTLPVPFKYGFAFDWHAFIVAGAIFLLSVFEA
                     VGDLTATAMVSDQPIEGEEYTKRLRGGVLADGLVSVIATALGSLPLTTFAQNNGVIQM
                     TGVASRHVGKYIAVILVLLGLFPVVGRAFTTIPSPVLGGAMVLMFGLIAIAGVRILVS
                     HGIRRREAVIAATSVGLGLGVAFEPEVFKNLPVLFQNSISAGGITAVLLNLVLPEDKT
                     EAAVKFDTDHLEH"
     gene            complement(1566..>1997)
                     /gene="rlmB"
                     /locus_tag="WIC03_001997"
     CDS             complement(1566..>1997)
                     /gene="rlmB"
                     /locus_tag="WIC03_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEZ3884449.1"
                     /translation="HNLGACLRTADAMGVHAVISPKDKSAGLNATVSKVACGAAETVP
                     YITVTNLARTLRELKEYGIWIIGTDMGGNADLYHCDLPDSAAWVMGNEGDGMRRLTRE
                     HCDMLVSIPMFGTVESMNVSVSAGMVLSETRRQRVLKNEKA"
BASE COUNT          335 a          465 c          658 g          539 t
ORIGIN      
        1 gtggtaaacg tgtggaccag acggatgccg tctgaaatgc aaattgaagc cgtgcggcag
       61 attcgctaca atccgcgctt ggatttttca acctttaaaa taaggaaata caatgagcgg
      121 tcagttgggc aaaggtgcgg atgcgcctga tttggtgtac ggtttggaag acaggccgcc
      181 gttcggtaat gcgctcttga gcgcggttac ccatcttttg gcgatttttg tgccgatgat
      241 tacgcccgcg ctgattgtgg gcggcgcgct ggaattgccg gtggagatga cggcgtatct
      301 cgtgtcgatg gcgatggttg cgtcgggtgt cggcacttat ttgcaggtca accgcttcgg
      361 gccggtcggt tcggggatgc tgtccatcca gtcggtgaat ttttcgttcg ttaccgtgat
      421 gattgcgctg ggcgcaggga tgaaggaggg cggtttgact aaggatgcga tgatttcgac
      481 gctcttgggt gtatcgtttg tcggcgcgtt tttggtgtgt ttctcggcgt ggcttttgcc
      541 gtatttgaaa aaagtgatta cgccgacggt cagcggcgtg gtcgtgatgc tcattggctt
      601 gagtttggta cacgtcggta ttaccgattt cggcggcggc ttcggcgcga aggcggacgg
      661 cacgttcggc tcgatggaaa acttggggct ggcttcgctg gtgttgctga ttgtgttgat
      721 attcaactgc atgaaaaacc cgctgttgcg gatgagcggc atcgcggtcg gtctgattgc
      781 cggttacatt gtcgcgctgt ttttgggcaa ggtggatttt tccgcgctgc aaaacctgcc
      841 gctggttacg ctgcccgtac cgtttaaata cggttttgct ttcgactggc acgcatttat
      901 tgtggcgggc gcgattttcc tgttgagcgt gtttgaggcg gtcggcgatt tgacggcgac
      961 ggcaatggtg tccgatcagc cgattgaagg cgaggaatac accaaacgcc tgcgcggcgg
     1021 cgtgttggca gacggcttgg tgtcggtgat tgcgacggct ttgggttcgc tgccgctgac
     1081 gacgtttgcg caaaacaacg gcgtgattca gatgaccgga gtggcttcgc gccatgtggg
     1141 caaatatatt gccgtgattt tggtgctgtt gggtttgttc cctgtggtcg gacgcgcgtt
     1201 tacgacgatt ccgagtccgg tgttgggcgg tgcgatggtt ttgatgttcg gcttgattgc
     1261 gattgcgggc gtgcggattt tggtcagtca cggcatccgc aggcgcgagg cggtgattgc
     1321 ggcaacgtcg gtcggcttgg gcttgggtgt cgcgtttgag ccggaagtgt ttaaaaacct
     1381 gcccgtcttg ttccaaaact ctatttccgc cggcggcatt acggcagtct tgctgaattt
     1441 ggtcttgccc gaagataaaa ccgaggcggc ggtcaagttt gataccgacc acttggaaca
     1501 ctgattttga aaacgaatgc cgtctgaaac agaatccctg tttcagacgg cattgttttt
     1561 gaggtttacg ctttttcgtt tttcaatacg cgttgtcggc gggtttcgct taataccatt
     1621 ccggcagaca cggagacgtt catgctttcg accgtgccga acatgggtat ggacaccagc
     1681 atgtcgcaat gttcgcgcgt gaggcggcgc ataccgtcgc cttcgttgcc catcacccat
     1741 gccgcgctgt cgggcagatc gcaatggtaa aggtcggcgt tgccgcccat atcggtgccg
     1801 atgatccaaa tgccgtattc tttcagctcg cgcagggtgc gggcgagatt ggttacggtg
     1861 atgtagggga cggtttccgc cgcgccgcag gcgactttgc tgacggtggc gttcagcccc
     1921 gcgcttttgt ctttcggcga gatgacggcg tgtacgccca ttgcgtcggc ggtacgcagg
     1981 cacgcgccga ggttgtg
//
LOCUS       DAWHSK010000290         1950 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.290, whole genome shotgun sequence.
ACCESSION   DAWHSK010000290 DAWHSK010000000
VERSION     DAWHSK010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.290"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            245..1702
                     /locus_tag="WIC03_001998"
     CDS             245..1702
                     /locus_tag="WIC03_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ3884450.1"
                     /translation="MSRHPAPTGEKTFFGHPFQLSTLFHIELWERFSFYGMQGILLIY
                     LYYTADKGGLGIDKTLAGGIVGAYSGSVYLSTILGAWFADRVWGAEKTLFLSGIVVML
                     GHIVLAAAPGLYGLLIGLIFIALGSGGVKSTASSMVGALYEQDEMRPLRDAGFSIFYI
                     AINIGGFLGPLLTGLLQENIGFHYGFGAAAVGMAFGLWRYSLGRKNLPHPTVPHPLSK
                     GQGKTAAAVGITLIAALATAIKTGLVNLDNFSGILLSTVILAVIAYFARLLTNPRVSS
                     DNKRHIIAYIPLFLTICMFWAVWFQIYTVATVYFDETVNRTIGSFTVPVAWKDSMQSL
                     WVILFSGLMAAMWTKMGRKQPKTPLKFAMAVFVTGASFLGFVPFISAGTPMPIAVFAL
                     IVLAITIGELMISPIALSISTKIAPPLFKTQMVALNFLAFSLGFTLGGVLFEKGYQAG
                     DEIGFYRLLFYIGATTGFLLLLLVPKLNKMLEGTD"
BASE COUNT          433 a          590 c          450 g          477 t
ORIGIN      
        1 accgccctat cggataagcc cttaacagaa aaggctttac ccgcgccgta tcggaacaca
       61 tcctctaaaa tacaatccgt tgaattgaaa aaaatataaa aacatccgcc cgcgaaaaac
      121 ggcagcgcgt cgtttgacaa agaatgaaaa tatcggttaa aaaccgattt tcatacaaaa
      181 aacaccgctg ccgtccgcat ccgtttcaga cggtattgag agaaaatctt ttaggagaac
      241 ctttatgtcc cggcatcccg cccccaccgg agaaaaaaca ttcttcggcc accccttcca
      301 gctttccacc ctcttccata tcgaattgtg ggaacgtttt tcattttacg gaatgcaggg
      361 catcctgctg atttacctct actacaccgc cgacaaaggc ggcttgggca tagacaaaac
      421 cctcgccggc ggcattgtcg gcgcatacag cggcagcgtg tacctgtcca ccattttggg
      481 ggcgtggttt gccgaccgag tatggggtgc ggaaaaaacc ctcttcctct cgggcatcgt
      541 cgtgatgctc ggacacatcg tccttgccgc cgccccgggc ctgtacggtc ttttaatcgg
      601 gctgatattc atcgcattgg gcagcggcgg tgtgaaatct acggccagtt ctatggtggg
      661 cgcattatac gaacaggacg aaatgcgccc gctgcgcgat gcgggatttt ccattttcta
      721 catcgccatc aacatcggcg gcttcctggg cccgctgctg accggcctgc tgcaggaaaa
      781 catcggtttc cattatggtt tcggcgcggc ggcggtcggt atggcattcg gcttgtggcg
      841 ttattccttg ggacgtaaaa acctgcccca ccccaccgtc ccccatccgc tttcaaaagg
      901 acagggcaaa actgcggccg ccgtcggcat caccctcatc gccgcacttg caaccgccat
      961 caaaaccggg cttgtcaacc tcgacaattt ctccggcatc ctcttatcta ccgtcatcct
     1021 tgccgtcatt gcctatttcg cccgcctgct gaccaacccc cgcgtcagtt ccgacaacaa
     1081 acggcacatc atcgcctaca tcccgctttt cctgaccatc tgtatgtttt gggccgtctg
     1141 gtttcagatt tacaccgtgg caaccgtcta tttcgacgaa accgtcaacc gcaccatcgg
     1201 ttcgtttacc gtgcccgtcg cctggaaaga ttccatgcaa agcctgtggg tcatcctgtt
     1261 ttccggactg atggcggcaa tgtggacaaa aatggggcgc aaacagccca aaaccccgct
     1321 gaaattcgct atggcggtat ttgttaccgg cgcgtcgttt ttgggattcg tcccctttat
     1381 ttccgccggc acgccgatgc ctattgcggt tttcgcactg attgtccttg ccatcacgat
     1441 aggcgaactg atgatttccc cgattgcgct gtccatctcc accaaaatcg caccgccttt
     1501 attcaaaacc caaatggtcg cccttaattt ccttgccttt tcattaggct tcactttggg
     1561 cggcgtattg tttgagaaag gctatcaggc gggcgacgaa atcggcttct accggctgct
     1621 gttctacatc ggcgcaacca caggcttcct gctgctcctg ctcgtcccca aattgaacaa
     1681 aatgctcgaa ggcacagact aagtcccgcc ccgatgcgcc gatgccgtct gaacccttca
     1741 gacggcattt ttccgcataa tgaaaccaaa ccgtttccac ccgacaggac aggctccccc
     1801 caaccggaag gcagcctgcc gattgtcatt tgaatatctg actgattccg tctgtttgca
     1861 agggaaatag ccgtttattt ccgcttgggc ggaaaccggt tctattgagt aaaaggcatt
     1921 ttatccgact aaattagtgc tgcatcaacg
//
LOCUS       DAWHSK010000291         1883 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.291, whole genome shotgun sequence.
ACCESSION   DAWHSK010000291 DAWHSK010000000
VERSION     DAWHSK010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.291"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(127..1437)
                     /locus_tag="WIC03_001999"
     CDS             complement(127..1437)
                     /locus_tag="WIC03_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEZ3884451.1"
                     /translation="MTDLFAREPDAPLAERLRPHTLDDVVGQEHLIGEGKSLRVAVEG
                     GKPHSMLLWGPPGVGKTTLARILAQSFNAQFLPVSAVFSGVKDIRGAIDKAEIALQQG
                     RATILFVDEVHRFNKAQQDAFLPYVENGLLTFIGATTENPSFEVNPALLSRAQVYVLQ
                     SLSSDDLKKLIAKVLALPEYQEFTIETDAQKLLVNTADGDARRLLNLLEQLLGAAETR
                     RLKTLTAEFLADSLGAQIRRFDKGGESFYNQISALHKSVRGSHPNAALYWFCRMLDGG
                     TDPRYLARRIVRIAWEDIGLADPRALQIANDAAATFERLGSPEGELALAQAVLYLAAA
                     AKSNAGYKAYNQMRRFVKENASDEVPVHLRNAPTKLMKELGYGREYRYAHDEPNAYAA
                     GESYMPDCLDEPDFYQPVPRGLEIKIGEKLEWLKSLDEEVLKAK"
BASE COUNT          454 a          493 c          480 g          456 t
ORIGIN      
        1 ttaatccact ataccacgtt gtctttattg gctttatcaa taaacaggat aagacctgaa
       61 aaaaagccga tacgcctttt tggtgtaccg gctttgccat actgttctgc ttcagacagc
      121 attgcttcat tttgccttta atacttcttc gtccagcgat ttcaaccatt ccagcttttc
      181 gccgattttg atttccaacc cgcgcgggac gggttggtag aagtccggtt cgtccaagca
      241 gtcgggcata tagctttcgc cggcggcgta ggcgttcggt tcgtcgtggg cgtagcggta
      301 ttcgcgtccg tagcccaatt ccttcatcag cttggtcggg gcattgcgca ggtggacggg
      361 tacttcgtcg ctggcgtttt ctttgacgaa acggcgcatt tgattgtatg ccttgtagcc
      421 cgcgttggat ttcgcggcgg cggcaagata caataccgct tgcgccaaag ccagttcgcc
      481 ttcgggcgag cctaagcgtt cgaaggtggc ggcggcatcg ttggcgattt gcaaggcgcg
      541 cgggtcggca agcccgatgt cttcccaagc gatacgcacg atgcggcggg cgaggtagcg
      601 cgggtcggtg ccgccgtcga gcatacggca gaaccaatac agcgcggcgt tcggatgcga
      661 accgcgcacg gatttgtgca gggcggagat ttggttgtag aaactctcgc cgcctttgtc
      721 gaaacggcgg atttgcgccc cgagactgtc ggcgagaaat tcggcagtca gggttttcag
      781 acggcgcgtt tcagcggcgc ctaacagttg ttccaacaaa ttcaacaatc tgcgcgcatc
      841 accgtcggcg gtattcacga gtaatttttg cgcatccgtt tcaatcgtaa actcttggta
      901 ttcaggcaaa gccaatacct tggcaatcag ctttttcagg tcgtctgaag acaaggattg
      961 caaaacatac acctgcgcgc ggctcaacag cgcgggattc acttcaaacg acggattttc
     1021 cgtcgttgcg ccaataaagg tcagcaaacc gttttccaca taaggcaaaa acgcatcctg
     1081 ctgcgccttg ttgaagcggt ggacttcatc gacaaacaaa atcgtcgccc tcccctgctg
     1141 caaagcgatt tcagccttgt cgattgcccc gcgtatgtct tttacgccgg aaaacacggc
     1201 ggaaacaggc agaaactggg cattgaaact ctgcgccaaa atccgcgcca acgtcgtctt
     1261 gcccacgccc ggcggccccc acagcaacat agaatgcggc ttgccccctt ctaccgccac
     1321 gcgcaaagat ttaccttcgc cgatgaggtg ttcctgcccc accacgtcgt caagcgtatg
     1381 cggacgcaat cgttcggcaa gcggcgcgtc gggttctcgg gcaaacaaat cggtcataac
     1441 ggctccgtca acaggttttc aaacaatatg attatacggc agggaacggc ggcgtaccgc
     1501 atacggattc cgcccctccg tttgccttaa gccgatatta ggcgcatact ggaaaagacg
     1561 agagacttca cacaatatat ccggcacgga gaccgattcc gcaccggcat gacaataccc
     1621 aaatcagcgt ttcaattaaa cattaaggag actaaaatag aaaatttgct ttatctacca
     1681 ttgctttgtt gatttaatcg gcattatgtt ttgaggcgga agcccatgaa tatactaata
     1741 ttcaagagat ggaatgggtg tctttatttt ctgatccgca aagagacgat gatagtctta
     1801 taacccttaa agatgaaaaa atcactgtaa aaaactatat tgtgccttgg tggaaaaaag
     1861 tgaaaacttt agaaaattag aac
//
LOCUS       DAWHSK010000292         1867 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.292, whole genome shotgun sequence.
ACCESSION   DAWHSK010000292 DAWHSK010000000
VERSION     DAWHSK010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.292"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..934)
                     /locus_tag="WIC03_002000"
     CDS             complement(<1..934)
                     /locus_tag="WIC03_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEZ3884452.1"
                     /translation="MAVFPLSAKHRKYALRALAVSIILVSAAYIASTERTERVRPQRV
                     EQKLPPLSWGGSGVQTAYWVQEAVQPGDSLADVLARSGMARDEIARITEKYGGEADLR
                     HLRADQSVHVLVGGDGGAREVQFFTDEDGERNLVALEKKGGIWRRSASEADMKVLPTL
                     RSVVVKTSARGSLARAEVPVEIRESLSGIFAGRFSLDGLKEGDAVRLMYDSLYFHGQQ
                     VAAGDILAAEVVKGGTRHQAFYYRSDKEGGGGGNYYDEDGRVLQEKGGFNIEPLVYTR
                     ISSPFGYRMHPILHTWRLHTGIDYAAPQGTPVRAS"
     gene            1012..>1867
                     /locus_tag="WIC03_002001"
     CDS             1012..>1867
                     /locus_tag="WIC03_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884453.1"
                     /translation="MKLTLAIPSLNLDEDETRIPLCLPAFNKILQYGSLHRQSCTASA
                     FYARYLWCGRLAERAAQSLNMPSEAVALATPVWQKMGMHQANVLTAEYLNVGTDEAER
                     LCRDLSAFYGDIPWRFVPVLPELWLVSLPCAYRWGAKPVLDLGGLLGADDQPDGEDAL
                     EWLRVQTEIQMWLAAHPVNHNRKKRGLPELNGLWLWDSLHGSSQGGTLFADTVWSRFH
                     PDRRALPDNFRAYEETAAYLPDTHHILFMDDLRLTALTGDRERYAAILQQWEERWFAP
                     LYEAVRIGK"
BASE COUNT          383 a          605 c          506 g          373 t
ORIGIN      
        1 cggaagccct gaccggcgtt ccctgcggtg cggcataatc gatgcccgtg tgcagccgcc
       61 aagtgtgcag gatggggtgc atacggtagc cgaacggcga agaaatgcgc gtatagacca
      121 gcggctcgat gttgaagccg cctttttcct gcaacaccct gccgtcttca tcgtaataat
      181 tgccgccccc tcctccttcc ttgtccgaac ggtaatagaa cgcctgatgc cttgtgccgc
      241 ccttaacgac ttcagccgcc aaaatatcgc ccgccgccac ctgctgcccg tggaaataca
      301 ggctgtcgta catcaggcgc acggcatcgc cttccttcaa accgtcaagg ctgaagcggc
      361 cggcgaaaat cccgcttaag gattcgcgga tttcgacggg cacttccgcc cgcgccagcg
      421 aaccgcgcgc cgacgttttg accacgaccg aacgcagcgt cggcaaaacc ttcatatccg
      481 cctcagaagc cgaccgccgc catatgccgc ctttcttttc caaagcgacc agattgcgct
      541 cgccgtcttc gtcggtaaaa aactgcactt cgcgcgcgcc gccgtcgccg ccgaccaaaa
      601 catgaaccga ctggtcggca cgcaaatgcc gcaaatcggc ttcgccgcca tatttttccg
      661 ttattcgggc aatttcgtcc cgcgccatac ccgaacgcgc cagcacgtcc gccagcgagt
      721 cgcctggctg caccgcctcc tgcacccaat atgccgtctg aacaccgctg ccgccccaag
      781 acagcggcgg cagtttttgt tccacgcgct gcggtctgac gcgctccgtc ctctctgtcg
      841 aagcaatgta tgccgccgac accaaaataa tcgaaacggc aagcgcgcgc agcgcgtatt
      901 tccgatgttt tgccgaaagt gggaagacag ccatattgtt cctttttgac ggcgtttgcg
      961 ccgaatcctc tataatgttg cgcctgacct gcatttctga aacgtcaaac gatgaaactc
     1021 accctcgcca ttccctcgct caatttggat gaagacgaaa cccgaatacc tctctgcctg
     1081 cccgccttca acaaaatcct gcaatacggc tcgctgcacc gccaaagctg cacggcttcg
     1141 gcattttacg cgcgttattt gtggtgcggc agactggcgg aacgcgcggc acaaagcctg
     1201 aatatgccgt ctgaagccgt cgcgctcgcc acgcccgtct ggcaaaagat ggggatgcat
     1261 caggcaaatg tgctaacggc ggaatatctg aacgtcggta cggatgaagc ggaacggctc
     1321 tgccgcgacc tgtcggcgtt ttacggcgac attccatggc gtttcgttcc cgtcctgccc
     1381 gaattatggc tggtttccct tccttgcgcg taccgctggg gcgcgaagcc cgttttggac
     1441 ttgggcggat tgttgggcgc ggacgatcag ccggacgggg aagacgcgtt ggaatggctg
     1501 cgcgtccaaa ccgaaatcca aatgtggctg gcggcgcacc ccgtcaacca taaccgcaag
     1561 aaacgcgggc tgcccgagtt gaacgggctg tggctttggg acagtctgca cggaagcagt
     1621 caaggcggca cgcttttcgc cgatacggtt tggagccgtt tccaccccga ccgccgcgca
     1681 ttgcccgaca acttccgcgc ctatgaagaa acggcggcat acctacccga cacgcaccat
     1741 atcctgttta tggacgattt gcgcctgacc gccctgacag gggacaggga gcggtatgcg
     1801 gcaatattgc agcagtggga agaacgctgg ttcgcaccgc tttacgaagc cgtccgcatc
     1861 ggcaaaa
//
LOCUS       DAWHSK010000293         1841 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.293, whole genome shotgun sequence.
ACCESSION   DAWHSK010000293 DAWHSK010000000
VERSION     DAWHSK010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.293"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..1612
                     /locus_tag="WIC03_002002"
     CDS             <1..1612
                     /locus_tag="WIC03_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pilus assembly/adherence protein PilC"
                     /protein_id="HEZ3884454.1"
                     /translation="SRDNGKHERNLGDIVNSPIVAVGGYLATSANDGMVHIFKQSGGD
                     KRSYNLKLSYIPGTMPRKDIENKESTLAKELRTFAEKGYVGDRYGVDGGFVLRQYKDR
                     VFMFGAMGFGGRGAYALDLSKIDSNNPTAVSLFDVKHDNSGNNGNNGNNRVELGYTVG
                     TPQIGKTHNGKYAAFLASGYATKKIDDPTNKTALYVYDLENNNGTPIATINVPDGKGG
                     LSSPTLVDKDLDGTVDIAYAGDRGGNMYRFDLSNNDPTKWSVRTIFKGTPDKPITSAP
                     AVSKLKDKRVVIFGTGSDLSEDDVDKKDIQSIYGIFDNDTGTDVAEEGQGKGLLEQHL
                     TQENKTLFLTDYKRSDGSGSKGWVVKLKDGQRVTVKPTVVLRTAFVTIRKYKDNGCGA
                     ETAILGINTADGGKLTKKSARPIVPEANTAVAQYSGHKQTAKGKSIPIGCMWKNNETV
                     CPNGYVYDKPVNVRYLDEKKTDGFSTTADGDAGGSGIDPDGKRSGKNNRCFSQKGVRT
                     LLMNDLDSLDITGPTCGMKRISWREIFY"
BASE COUNT          513 a          461 c          488 g          379 t
ORIGIN      
        1 cagccgcgac aacggcaagc acgagcgcaa tttgggcgac atcgtcaaca gccccatcgt
       61 ggcggtcggc gggtatttgg ctacttccgc caacgacggg atggtgcata tcttcaaaca
      121 aagcggcggg gacaagcgca gctacaatct gaagctcagt tatatcccgg gtacgatgcc
      181 gcgcaaggat attgaaaaca aagaatccac ccttgccaaa gagctgcgca cctttgccga
      241 aaaaggctat gtgggcgacc gctacggcgt ggacggcggc tttgttttgc gccaatacaa
      301 agatcgcgtg tttatgttcg gcgcgatggg ctttggcggt agaggcgcat acgccttgga
      361 tttaagcaaa atcgacagca ataacccgac cgccgtttcc ctgtttgatg tcaaacatga
      421 caatagtggc aataatggca ataacggcaa taatcgcgtg gaattaggct acaccgtcgg
      481 cacgccgcaa atcggcaaaa cccacaacgg caaatacgcc gctttcctcg cctccggtta
      541 tgcgactaaa aaaattgacg acccaacaaa taaaaccgcg ctgtatgtgt atgatttgga
      601 aaacaacaac ggtacgccga ttgcaacaat caacgtaccc gacggcaagg gcgggctttc
      661 gtcccccacg ttggtggata aagatttgga cggcacggtc gatatcgcct atgccggcga
      721 ccgcggcggg aatatgtacc gctttgattt gagcaacaac gatccgacca aatggtctgt
      781 acgtactatt tttaaaggca cgccggataa gccgattacc tccgcgcccg ccgtttccaa
      841 actgaaagac aaacgcgtgg ttatcttcgg tacgggcagt gatttgagtg aggatgatgt
      901 tgataaaaag gatatacaat ctatttacgg tatttttgac aatgacacag gcacggatgt
      961 ggcagaagaa ggacagggca aagggttgct cgagcaacac cttactcagg aaaataaaac
     1021 cttattcctg accgattaca agcgatccga cggatcgggc agcaaagggt gggtagtgaa
     1081 attgaaggac ggacagcgcg ttaccgtcaa accgaccgtg gtattgcgta ccgcctttgt
     1141 aaccatccgc aaatataaag acaacggctg cggcgcggaa accgctattt tgggcatcaa
     1201 taccgccgac ggcggcaagc tgaccaagaa aagcgcgcgc ccgattgtgc cggaagccaa
     1261 tacggctgtc gcgcaatatt ccggtcataa gcaaaccgcc aaaggcaaat ccatccctat
     1321 aggttgtatg tggaaaaaca atgaaaccgt ctgcccgaac ggatatgttt acgacaaacc
     1381 ggttaatgtg cgttatctgg atgaaaagaa aacagacgga ttttcaacaa cggcagacgg
     1441 cgatgcgggc ggcagcggta tagaccccga cggcaagcgt tccggcaaaa acaaccgctg
     1501 cttctcccaa aaaggggtgc gcaccctgct gatgaacgat ttggacagct tggacattac
     1561 cggcccgacg tgcggtatga aacgaatcag ctggcgtgaa atcttctact gatttgcacg
     1621 cgaaaatgcc gtccgaaagg ttttcggacg gcattttttg cgtttttcgg gaggggcggg
     1681 ttcgtaaaag gcgggctata gggtaggctt catctcgcca atctcactga atccatcaat
     1741 ttccacaatt caattaaata ccgtcaaacc gatgccgtca ttcccgcgca ggcgggaatc
     1801 tagaccttgg gataacagca atattcaaag gttagctgaa g
//
LOCUS       DAWHSK010000294         1840 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.294, whole genome shotgun sequence.
ACCESSION   DAWHSK010000294 DAWHSK010000000
VERSION     DAWHSK010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.294"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..498)
                     /locus_tag="WIC03_002003"
     CDS             complement(<1..498)
                     /locus_tag="WIC03_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEZ3884455.1"
                     /translation="MRRFLPIAAICAVVLLYGLTAATGSTSSLADYFWWIVAFSAMLL
                     LVLSAVLARYVILLLKDRRDGVFGSQIAKRLSGMFTLVAVLPGVFLFGVSAQFINGTI
                     NSWFGNDTHEALERSLNLSKSALNLAADNALGNAVPVQIDLIGAASLPGDMGRVLEHY
                     AGSGFA"
     gene            complement(498..1097)
                     /locus_tag="WIC03_002004"
     CDS             complement(498..1097)
                     /locus_tag="WIC03_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HEZ3884456.1"
                     /translation="MAFITRLFKSIKQWLVLLPMLSVLPDAAAEGIDVSRAEARITDG
                     GQLSISSRFQTELPDQLQQALRRGVPLNFTLSWQLSAPIIASYRFKLGQLIGDDDNID
                     YKLSFHPLTNRYRVTVGAFSTDYDTLDAALRATGAVANWKVLNKGALSGAEAGETKAE
                     IRLTLSTSKLPKPFQINALTSQNWHLDSGWKPLNIIGNK"
     gene            complement(1063..>1840)
                     /gene="rsmB"
                     /locus_tag="WIC03_002005"
     CDS             complement(1063..>1840)
                     /gene="rsmB"
                     /locus_tag="WIC03_002005"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEZ3884457.1"
                     /translation="HNIAAALQSHPPMTLRVNRRHGNAESYLEKLAAEGIAAKALDEY
                     AVTLEEAVPVNRLPGFSDGIVSVQDFGAQQAAYLLNPKDGERILDACAAPGGKTGHIL
                     ELADCRVTALDIDAGRLKRVEDNIARLGFQTASAACADARDLAAWYDGKPFDAILADV
                     PCTASGVARRNPDVKWLRRPTDALKTARQQEALLDALWQTLKQGGRMLLATCSVFVEE
                     NDGQLQKFLNRHADAEPIESRVLLPNKHQDGFYYALIQKH"
BASE COUNT          390 a          539 c          531 g          380 t
ORIGIN      
        1 ggcaaaaccg ctgccggcgt aatgttccag caccctgccc atatccccgg gcagggaagc
       61 cgcgccgatg aggtctatct gcacggggac ggcgttgccg agggcgttgt ctgccgccag
      121 attcaatgcg gacttgctca aattgaggct gcgttcaagc gcctcgtggg tatcgttgcc
      181 gaaccacgaa ttaatcgtgc cgttgatgaa ctgtgcggaa acgccgaaca gaaacacgcc
      241 gggcagtacg gcaaccagcg taaacatccc ggaaaggcgt ttggcaatct gcgaaccgaa
      301 tacgccgtcg cgcctgtctt tcaacagcaa tatgacataa cgtgccaaaa cggcggacaa
      361 caccagcagc agcattgcgc tgaacgcaac aatccaccag aaataatccg ccagcgaact
      421 ggtgctgccg gttgccgccg tcagtccgta caacaggacg acggcgcata tggctgcgat
      481 cggtagaaaa cggcgcatta tttgttcccg atgatgttta gaggtttcca acccgaatcc
      541 aaatgccagt tttgagaagt caatgcattg atttgaaaag gcttgggcag ttttgaagtg
      601 gacagcgtca ggcggatttc cgccttggtt tcccctgctt ccgcaccgga cagcgcgcct
      661 ttgttcagga ctttccagtt ggcaaccgcg ccggtcgcgc gcaatgccgc atccaaggtg
      721 tcgtagtctg tcgaaaacgc gccgacggta acgcggtagc ggttggtcag cggatggaaa
      781 ctcagtttgt agtcaatatt gtcgtcatcg ccaatcagtt gccccaattt aaaccgataa
      841 gaagcgatta tcggggcgga aagctgccag cttaaggtaa agttgagcgg cacgccccgg
      901 cgcaacgcct gttggagctg gtcgggcagc tcggtttgga agcggctgct gatggaaagc
      961 tgcccgccgt cggttatcct cgcttcggcg cggctcacat ctatcccctc cgccgccgcg
     1021 tccggcaaaa cggaaagcat cggcagcagc acaagccatt gtttaatgct tttgaataag
     1081 cgcgtaataa aagccatctt ggtgtttgtt cggtaaaagc acccgcgatt cgatcggttc
     1141 ggcatcggca tggcggttga ggaatttttg caattgcccg tcgttttctt cgacgaacac
     1201 ggaacaggtg gcaagcagca ttctgccgcc ctgtttcagc gtctgccaaa gtgcgtccag
     1261 cagggcttcc tgctggcggg cggttttgag cgcgtcggtc ggacggcgca gccatttcac
     1321 gtcgggattg cgccgcgcca cgccggaagc ggtgcatggc acgtcggcaa ggattgcgtc
     1381 gaaaggcttt ccatcatacc acgccgccaa atcccgcgca tcggcacacg ccgccgatgc
     1441 cgtctgaaag cccaaacggg cgatattgtc ctccacacgc ttcagacggc ctgcatcaat
     1501 gtctaaggcg gtaacgcggc aatccgccag ttccaagata tgccccgtct tgccgcccgg
     1561 cgcggcgcac gcgtccaaaa tccgttcgcc gtctttcggg ttaaggagat acgccgcctg
     1621 ctgcgcgccg aagtcctgta ccgaaacgat gccgtctgaa aaaccgggca ggcggtttac
     1681 cggcacggct tcttccaacg taaccgcata ttcgtccaac gccttagccg caatgccttc
     1741 cgcagccagt ttttccaaat agctttcggc gttgccgtgc cggcggttga cgcgcaacgt
     1801 catcggcgga tgggattgca gcgcggcggc gatgttgtgc
//
LOCUS       DAWHSK010000295         1822 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.295, whole genome shotgun sequence.
ACCESSION   DAWHSK010000295 DAWHSK010000000
VERSION     DAWHSK010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.295"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            212..1765
                     /locus_tag="WIC03_002006"
     CDS             212..1765
                     /locus_tag="WIC03_002006"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEZ3884458.1"
                     /translation="MTQANRVIIFDTTLRDGEQSPGAAMTKEEKIRVARQLEKLGVDI
                     IEAGFAAASPGDFEAVNAIAKTITKSTVCSLSRAIERDIRQAGEAVAPAPKKRIHTFI
                     ATSPIHMEYKLKMKPKQVIEAAVKAVKIAREYTDDVEFSCEDALRSEIDFLAEICGAV
                     IEAGATTINIPDTVGYSIPYKTEEFFRELIVKTPNGGKVVWSAHCHNDLGLAVANSLA
                     ALKGGARQVECTVNGLGERAGNASVEEIVMALKVRHDLFGLETGIDTTQIVPSSKLVS
                     TITGYPVQPNKAIVGANAFSHESGIHQDGVLKHRETYEIMSAESVGWATNRLSLGKLS
                     GRNAFKTKLADLGIELESEEALNAAFARFKELADKKREIFDEDLHALVSDEMGSMNAE
                     SYKFISQKISTETGEEPRADIVFSIKGEEKRASATGSGPVDAIFKAIESVAQSGATLQ
                     IYSVNAVTQGTESQGETSVRLARGNRVVNGQGADTDVLVATAKAYLSALSKLEFSAAK
                     PKAQGSGTI"
BASE COUNT          468 a          506 c          500 g          348 t
ORIGIN      
        1 ttatttcggc ggcgtgaacg cgcttgccga agcgttgaaa cagcacgatc ccgaaaatgc
       61 cgcgacgacc gccgccatct ataaatggcg cacgcgcggg tcgctgcctt tggcgcaact
      121 gcaaaagctg accgcgcttg ccgaatcgca aggcaggccg ctggatttga atgctttttt
      181 acaaaaaaac gaatctctgg agagaacaga aatgacacag gccaaccgcg ttatcatttt
      241 cgacaccacc ctgcgcgacg gcgaacaatc gcccggcgcc gctatgacca aagaggaaaa
      301 aatccgcgtc gcccgccagc tggaaaaatt gggtgtggac atcatcgaag cgggttttgc
      361 cgctgccagc ccgggcgatt tcgaggcggt caatgcgatt gcgaaaacca ttaccaaatc
      421 aacggtctgt tcattgtccc gtgccatcga gcgggacatc cgtcaggcgg gtgaggccgt
      481 tgcgcccgcg ccgaaaaaac gcatccacac cttcatcgcc accagcccca tccatatgga
      541 gtacaaattg aagatgaagc cgaagcaggt gattgaggcg gcggtcaaag cggtgaaaat
      601 cgctcgtgaa tacaccgacg atgtggaatt ttcctgcgaa gacgcgttgc gttcggaaat
      661 cgatttcctt gccgaaatct gcggcgcggt aattgaagcg ggcgcgacca ccatcaatat
      721 tcccgatacc gtcggctatt ccatcccgta caaaaccgaa gaatttttcc gcgaactgat
      781 tgtcaaaacg cccaacggcg gcaaagtcgt ttggtcggca cactgccaca acgatttggg
      841 cttggcggtt gccaattcgc ttgccgcatt aaaaggcggt gcgcgtcagg tggaatgtac
      901 tgtcaacggc ttgggcgaac gtgcaggcaa tgcttcggtt gaagaaatcg tgatggcgtt
      961 gaaagtgcgc cacgacttgt tcggcttgga aaccggcatc gataccacgc aaatcgtgcc
     1021 ttcgtccaaa ctggtgtcca ccattacggg ctatcccgtg cagcccaaca aagccattgt
     1081 cggtgccaat gccttttcgc atgaatcggg catccatcag gacggggtgc tgaaacaccg
     1141 cgaaacttac gagattatgt ccgccgaatc ggtcggctgg gcaacaaacc gtttgagctt
     1201 gggcaaattg tccggccgca acgccttcaa aaccaagctg gcggatttgg gcatcgagtt
     1261 ggaaagcgaa gaggcactga acgccgcgtt tgcccgcttc aaagaactcg ccgacaaaaa
     1321 acgcgaaatc ttcgatgaag acctgcacgc actggtatcc gacgaaatgg gcagcatgaa
     1381 tgccgagagc tacaaattca tctcccaaaa aatcagcacc gaaaccggcg aagagccgcg
     1441 cgccgacatc gtgttcagca tcaaaggcga agaaaaacgc gcttccgcaa ccggttccgg
     1501 tccggttgat gcaattttca aagcgattga aagcgtggcg caaagcggcg cgactttgca
     1561 gatttattcc gtcaacgccg tcacgcaagg tacggaaagc caaggcgaaa ccagcgtccg
     1621 tctggcacgc ggcaaccgcg tcgtcaacgg tcagggcgcg gataccgacg ttttggttgc
     1681 cactgccaaa gcctaccttt ccgctttgag caaactggaa tttagtgccg ccaaaccgaa
     1741 agcgcaaggc agcggtacga tttgagcgtg aaaacagacg atgccgtctg aagcataaaa
     1801 aggcttcaga cggcattgcg gc
//
LOCUS       DAWHSK010000296         1807 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.296, whole genome shotgun sequence.
ACCESSION   DAWHSK010000296 DAWHSK010000000
VERSION     DAWHSK010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.296"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(47..1558)
                     /gene="ubiB"
                     /locus_tag="WIC03_002007"
     CDS             complement(47..1558)
                     /gene="ubiB"
                     /locus_tag="WIC03_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HEZ3884459.1"
                     /translation="MKWLKRLTVIVGTFYRYRLAGLCASLMGSGWICALLKMMPQSSK
                     LKNEPPAVCLRLALESLGPIFIKFGQVLSTRPDLIPHDYAVELAKLQDKVPPFDARLS
                     REQIEKSLGQPIEKLYAEFETEPIASASIAQVHKARLHSGEQVAVKVLRPNLLPVIEQ
                     DLSLMRFGAGWVERLFADGKRLKPREVVAEFDKYLHDELDLMREAANASQLGRNFQNS
                     DMLIVPKVFYDYCTSDVLTIEWMDGTPVSDIAKLKADGIDLHKLADYGVEIFFTQVFR
                     DGFFHADMHPGNILVAADNRYIALDFGIVGTLTDYDKRYLAINFLAFFNRDYRRVATA
                     HIESGWVPADTRAEELEAAVRAVCEPVFNKPISQISFGLVLMRLFEVSRRFNVEIQPQ
                     LVLLQKTLLNIEGLGRQLDPDLDLWKTAKPFLVKWMNGQVGPKALWRNLKNEAPDWAQ
                     IIPSLPRKISALIDENRQQEMRDAYIHLVKVQQRQSLWLAAIAVVLLLILLLK"
     gene            complement(1593..1805)
                     /locus_tag="WIC03_002008"
     CDS             complement(1593..1805)
                     /locus_tag="WIC03_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease III domain protein"
                     /protein_id="HEZ3884460.1"
                     /translation="MELETVVPPEEGEGEGEGADGEMPEYLELHGGREDDISAPSQPE
                     PPTRNIKPLLFGLLIAFLGYLLGKIF"
BASE COUNT          421 a          498 c          489 g          399 t
ORIGIN      
        1 ggtttcctta taaacgggcg gagtcgggca atgattcgga caaaacctat ttcaaaagca
       61 aaatcagcag caaaacaacc gctatcgccg ccagccacag gctttgccgc tgctgcactt
      121 tgaccaaatg gatataggca tcacgcattt cctgctggcg gttttcatca atcaacgcac
      181 tgattttgcg cggcaatgaa ggaatgattt gcgcccagtc gggggcttcg tttttgaggt
      241 tgcgccaaag ggctttaggg ccgacctgcc cgttcatcca tttcaccaaa aacggtttgg
      301 cggttttcca caagtccaaa tcgggatcaa gctgccgtcc caagccttcg atgttgagca
      361 gcgttttttg cagcaatacc agctgcggct ggatttcgac attgaagcgg cggctgactt
      421 caaacaggcg catcagcacc aagccgaagg aaatctgcga aatcggtttg ttgaacactg
      481 gttcgcacac ggcgcggaca gccgcttcca actcttccgc gcgcgtgtcg gcgggcaccc
      541 agcccgattc aatgtgggcg gtggcgacgc gccggtaatc gcggttgaag aaggcgagga
      601 agttgatggc gagataacgt ttgtcgtaat cggtcagcgt gccgacgatg ccgaaatcga
      661 gggcgatgta gcggttgtcg gcggcaacca aaatattgcc ggggtgcata tccgcgtgga
      721 aaaaaccgtc gcggaagacc tgcgtgaaaa agatttccac gccgtaatcg gcgagtttgt
      781 gcaaatcgat gccgtctgct ttgagtttgg cgatgtcgga aaccggcgtg ccgtccatcc
      841 attcgatggt cagcacgtcg ctggtgcagt agtcgtaaaa caccttcggc acaatcagca
      901 tatcgctgtt ttggaaattg cgtccgagct ggctggcatt ggcggcttcg cgcatcaagt
      961 ccaactcgtc gtgcagatat ttgtcgaact ccgccaccac ttcgcgcggc ttcagacgct
     1021 tgccgtcggc aaacagacgc tcgacccagc ctgcaccaaa gcgcatcagc gacaaatcct
     1081 gttcgatcac gggcaaaagg ttggggcgca agactttgac cgccacttgt tcgcccgaat
     1141 gcaggcgggc tttgtgtact tgggcgatgg acgcgctggc gatgggctcg gtttcaaatt
     1201 ccgcatacag cttttcgatg ggctgaccca gcgatttttc gatttgttca cgcgaaagcc
     1261 gcgcgtcaaa aggcggcact ttgtcttgca gctttgccag ttcgacggcg taatcgtgcg
     1321 gaatcaaatc ggggcgtgtg gacaaaacct gcccgaactt gatgaaaatc ggccccaggc
     1381 tttccaaggc aaggcgcaga cagacggcag gcggttcgtt tttcaatttg gacgactgcg
     1441 gcatcatttt cagcagagcg catatccaac cgctacccat cagcgaagca cacagacctg
     1501 ccagccgata gcggtaaaaa gtcccgacaa tgaccgtcag gcgtttcaac catttcatat
     1561 ataaaatccc gaatacagca tttatcggac aatcaaaaaa tcttaccgag cagatagccc
     1621 aaaaaggcaa tcagcagccc gaaaagcagc ggtttgatat tgcgggtcgg cggttcgggt
     1681 tgcgaaggtg cggaaatatc atcttcccgc ccgccatgaa gttcaagata ttcgggcatt
     1741 tccccatccg cgccctctcc ttcgccctca ccttcctccg gcggcacgac cgtttctaac
     1801 tccatat
//
LOCUS       DAWHSK010000297         1777 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.297, whole genome shotgun sequence.
ACCESSION   DAWHSK010000297 DAWHSK010000000
VERSION     DAWHSK010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.297"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            54..935
                     /gene="nadC"
                     /locus_tag="WIC03_002009"
     CDS             54..935
                     /gene="nadC"
                     /locus_tag="WIC03_002009"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEZ3884461.1"
                     /translation="MPSENTLFSLPDTLLRPIVEQALSEDLGRRGDITSAAVIAPDKT
                     AKLFLVSREDGVIAGMDLARLAFQTMDPSVRFQAEIRDGQVVRAGQTLAAVEGNACAL
                     LAAERTALNYLTHLSGIATATARAVAEVAKYGTDIVCSRKTIPLLRVLQKYAVRAGGG
                     VNHRMGLDDAILVKDNHLAYCGSIAQAVRQAKQAVGPLTCVEIEVDTLAQLDEAIAAG
                     AERILLDNMDDETLKEAANRCHTQTAHPHTVYCEASGGIGFDRLKRVAQTGVDGIALG
                     YLTHSSRSLDIGLDFLA"
     gene            963..1249
                     /locus_tag="WIC03_002010"
                     /pseudo
     CDS             963..1249
                     /locus_tag="WIC03_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346305.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1341..1622
                     /locus_tag="WIC03_002011"
     CDS             1341..1622
                     /locus_tag="WIC03_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEZ3884462.1"
                     /translation="MNTIPLHTILKLMAHPERMAILIQLLDSERNIAELAKSLSLPAT
                     AVSNHLSRLRVEGLVDFTRYHRIIEYRLISEDAATILRTIRDLENKRAV"
     gene            1682..>1777
                     /locus_tag="WIC03_002012"
     CDS             1682..>1777
                     /locus_tag="WIC03_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEZ3884463.1"
                     /translation="MKITTWNVNSLNVRLPQVQNLLADNPPDILVL"
BASE COUNT          431 a          519 c          455 g          372 t
ORIGIN      
        1 atgaagtttt gccccatcgg gcaatatcaa tctttttcaa caaaggaaac accatgccgt
       61 ctgaaaacac cctcttttcc ctacccgaca ccctgttgcg ccccatagtc gaacaagcct
      121 tgagcgaaga tttgggcagg cgcggcgata ttacgtccgc cgccgtcatc gcccccgaca
      181 aaaccgccaa actcttcctt gtcagccgcg aagacggcgt tatcgccggc atggacttgg
      241 cgcgcctcgc ctttcagacg atggatccgt ccgtccgctt ccaagccgaa atccgagacg
      301 ggcaagtcgt ccgcgcaggt cagacgcttg ccgccgtcga aggcaacgcc tgcgcgctgc
      361 tcgccgccga acgcaccgcg ctcaactacc tcacgcactt aagcggcatc gccaccgcca
      421 ccgcgcgtgc cgttgccgaa gtcgccaaat acggtacaga catcgtgtgc agccgcaaaa
      481 ccatccccct gctgcgcgtc ctgcaaaaat acgccgtcag ggcgggcggc ggcgtgaacc
      541 accgcatggg tttggacgat gccatccttg tcaaagacaa ccacctcgcc tattgcggca
      601 gcatcgccca agccgtgcgg caggcaaaac aggcggtcgg accgttgact tgtgtagaaa
      661 tcgaagtgga cacgttggca caactggacg aagccatcgc agcgggcgcg gaacggattt
      721 tgctggacaa catggacgac gaaaccctga aagaagcggc aaaccgctgc cacacgcaaa
      781 ccgcccaccc ccacaccgtc tattgcgaag catcgggcgg catcggcttc gaccgcctga
      841 agcgcgtggc gcaaaccggc gtggacggca tcgccctcgg ctatctgacc cacagcagcc
      901 gctcgttgga cataggtttg gatttcttgg catgagtttt agggtgtcgg cagttgtctg
      961 atatgtcaga caaggaaccg cctaaccgta atccggttat tgcctcaagg aggaaatgcc
     1021 gtctgaaata ttcttcagac ggcatttttc gtgaaggtcg tgatgcttta gaaaaaacag
     1081 cgtttcaggc aggtattttg tttgcccgac agcgcggagg catcggtagg gcaggggaaa
     1141 ggacgggggc ggcagtttta tgccgtctga aagcccgcct ttacgcgtgt ttgcaaaaaa
     1201 agtgggaaaa ggaacataca atcctgtaca atcatccata aatatttgat ttataataag
     1261 atttataaag ataatcacaa tcatccatat ctgtcgcccg tcaatccgct tggcgggcgg
     1321 caaaggtttt aggaataccg atgaacacaa taccgctcca caccatactc aaacttatgg
     1381 cgcatcccga acgtatggcg atactgatcc aactgttgga cagcgaacgc aatatcgccg
     1441 aactggcaaa atccttatcc ctgccggcca ccgccgtttc caaccatttg agccgactgc
     1501 gcgtggaagg tctggtggac tttacgcgtt accaccgcat tatcgaatac cgcctgattt
     1561 ccgaagacgc ggcaacgatt ctgcggacaa tccgcgattt ggaaaacaaa cgcgcggtat
     1621 agtgttaaaa tcctttcctt ttgccgtctg aacgtttcag acggcatttt tcggaaatgt
     1681 tatgaaaatc accacttgga acgtcaattc gctcaatgtg cggctgccgc aggtgcaaaa
     1741 cctgcttgcc gacaatccgc ccgatatttt ggttttg
//
LOCUS       DAWHSK010000298         1777 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.298, whole genome shotgun sequence.
ACCESSION   DAWHSK010000298 DAWHSK010000000
VERSION     DAWHSK010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.298"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..78)
                     /locus_tag="WIC03_002013"
     CDS             complement(<1..78)
                     /locus_tag="WIC03_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HEZ3884464.1"
                     /translation="MKPAVWAALLLCACTSNFGDREHQFL"
     gene            281..1609
                     /gene="miaB"
                     /locus_tag="WIC03_002014"
     CDS             281..1609
                     /gene="miaB"
                     /locus_tag="WIC03_002014"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEZ3884465.1"
                     /translation="MKKVFIRTFGCQMNEYDSDKMLAVLAEEHGGIEQVTQADEADII
                     LFNTCSVREKAQEKVFSDLGRVRPLKEKNPGLIIGVAGCVASQEGENIIKRAPYVDVV
                     FGPQTLHRLPKMIVDKETSGLSQVDISFPEIEKFDHLPPARVEGGAAFVSIMEGCSKY
                     CSFCVVPYTRGEEFSRPLNDVLTEIANLAQQGVKEINLLGQNVNAYRGEMDDGEICDF
                     ATLLRIVHEIPGIERMRFTTSHPREFTDSIIECYRDLPKLVSHLHLPIQSGSDRVLSA
                     MKRGYTALEYKSIIRKLRAIRPDLCLSSDFIVGFPGETEREFEQTLKLVKDIAFDLSF
                     VFIYSPRPGTPAANLHDDTPHEEKVRRLEALNEVIEAETARINQTMIGTVQRCLVEGI
                     SKKDPDQLQARTANNRVVNFTGTPDMINQMIDLEITEAYTFSLRGKVVEA"
BASE COUNT          461 a          579 c          397 g          340 t
ORIGIN      
        1 caggaactga tgttccctgt cgccgaaatt gctggtacac gcacacagca gcaatgccgc
       61 ccacacagcc ggtttcatac acacctccca ttaaagccaa acattataca gccgtcccga
      121 ccgattaaat tcatatttta aaacaatatt ctacctccca aacccacatc gtgctataat
      181 ccgcaccgat tttcagacgg catcgtcgtg ccgtctgaaa ttttttcatt ccaacaacaa
      241 tcagccccgc gattacggct gcctgagaaa gacacaaacc atgaaaaaag tatttatccg
      301 caccttcggc tgccagatga acgaatacga cagcgacaaa atgctcgccg tcctcgccga
      361 agaacacggc ggcatcgaac aggttaccca agccgacgaa gccgacatca tcttgttcaa
      421 cacctgctcc gtgcgcgaaa aagcgcaaga aaaagtcttc tccgatttgg ggcgcgtgcg
      481 tccgctcaaa gaaaaaaacc ccggcctcat catcggcgtt gccggctgcg tcgcctcgca
      541 agaaggcgaa aacatcatca aacgcgcgcc ttatgtggac gtggttttcg gcccgcaaac
      601 gctgcaccgc ctgccaaaaa tgattgtgga caaagaaacc agcgggctgt cgcaagtcga
      661 tatttccttc cccgaaatcg aaaaattcga ccacctgccg cccgcccgcg tcgaaggcgg
      721 cgcggcattt gtatcgatta tggaaggctg ttccaaatac tgctccttct gcgtcgtccc
      781 ctacacgcgc ggcgaagaat tctcccgccc gctcaacgac gtattgacag aaatcgccaa
      841 ccttgcccag caaggcgtta aagaaatcaa cctcttggga caaaacgtca acgcctatcg
      901 cggcgaaatg gacgacggcg aaatctgcga cttcgccacc ctgctgcgca tcgtccacga
      961 aatccccggc attgaacgta tgcgcttcac caccagccac ccgcgcgagt ttaccgactc
     1021 gattatcgag tgctaccgcg acctgcccaa attggtttcc cacctgcacc tgccgattca
     1081 aagcggttcc gaccgcgtat tgagcgcgat gaaacgcggc tacaccgctt tggaatacaa
     1141 atccatcatc cgcaaactgc gtgccatccg tcctgattta tgcctgagca gcgatttcat
     1201 cgtcggcttc cccggcgaga ccgaacgcga gttcgagcaa accttgaaac tggtgaaaga
     1261 cattgccttc gacttgagct tcgtgtttat ttacagtccg cgccccggca cgcctgccgc
     1321 caacctgcac gacgacacgc cgcacgaaga aaaagtgcgc cgcctcgaag ccttgaacga
     1381 agtcatcgaa gccgaaaccg cgcgcatcaa ccaaacgatg atcggcacgg ttcaacgctg
     1441 cctggtcgaa ggcatctcca aaaaagaccc cgaccagctc caagcccgca ccgccaacaa
     1501 ccgcgtcgtc aactttaccg gcacgcccga catgattaac caaatgatcg atttggaaat
     1561 caccgaggcc tacaccttct ccctgcgcgg caaagttgtc gaagcctaaa cccttacgcc
     1621 gaaaaaatgc cgtctgaagc gtttcagacg gcattttgcc ttgtatcggc agacgatggc
     1681 gcggccaggc ggcttaattt gccgcatccc gatccgacaa ccacgcgcgc acacgccgtt
     1741 ccaccgcttc ggcactcaag cccaaatcgt ctaaaag
//
LOCUS       DAWHSK010000299         1763 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.299, whole genome shotgun sequence.
ACCESSION   DAWHSK010000299 DAWHSK010000000
VERSION     DAWHSK010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.299"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            123..1496
                     /locus_tag="WIC03_002015"
     CDS             123..1496
                     /locus_tag="WIC03_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEZ3884466.1"
                     /translation="MSNPFSSLGLGTELVSALTAQGYENPTPIQAAAIPKALAGHDLL
                     AAAQTGTGKTAAFMLPSLERLKRYATASTSPAMHPVRMLVLTPTRELADQIDQNVQGY
                     IKNLPLRHTVLFGGMNMDKQTADLRAGCEIVVATVGRLLDHVKQKNIHLNKVEIVVLD
                     EADRMLDMGFIDDIRKIMQMLPRQRQTLLFSATFSAPIRKLAQDFMNAPETVEVAAQN
                     TTNANVEQHIIAVDTIQKRNLLERLIVDLHMNQVIVFCKTKQSVDRVTRELVRRNLSA
                     QAIHGDRSQQSRLETLNAFKDGNLRVLVATDIAARGLDIAELPFVINYEMPAQPEDYV
                     HRIGRTGRAGADGVAISLMDESEQKMFESIKELTGNKLLIERIEGFEPQWWEQGGAKP
                     EKPEMREPRQRNRYESAKAQREKNTRPENAANDAGAACGKIAGRSRRSRREHRTCALL
                     QPRYGVK"
BASE COUNT          438 a          578 c          423 g          324 t
ORIGIN      
        1 cgtgctattt gtactgtctg cggctcgccg ccttgtcctg atttttgtta atccactata
       61 tttccccgtc ctatcggttt cccgtttcag acgacataag gtctgaaaga aagactacaa
      121 ttatgagtaa tccattttcc tctttaggtt tgggtacgga actcgtttcc gcactgaccg
      181 cgcaaggtta cgaaaacccg acgcccatcc aagccgccgc cattcccaaa gcactcgccg
      241 gtcatgattt actagccgcc gcgcaaaccg gcacgggcaa aaccgccgcc tttatgctgc
      301 cctcgctcga acgcctcaag cgttacgcca ccgccagcac ctcgcccgcg atgcaccccg
      361 tgcgtatgct cgtcctcacc cccacgcgcg aacttgccga ccaaatcgac caaaacgtgc
      421 agggctacat caaaaacctg ccgctgcgcc acaccgtctt gttcggcggt atgaatatgg
      481 acaaacagac cgccgacctg cgtgccggct gcgaaatcgt cgtcgccacc gtcggacggc
      541 tgctcgacca cgtgaaacag aaaaacatcc atttgaacaa agtcgaaatc gtcgttttgg
      601 acgaagccga ccgtatgctg gatatgggtt ttatcgacga catccgcaaa atcatgcaga
      661 tgctgccccg ccaacgccaa accctgctct tttccgccac cttctccgcc ccgatacgca
      721 aactggcgca agacttcatg aacgcgcccg aaaccgtcga agtcgccgcg caaaacacca
      781 ccaacgccaa cgtcgagcag cacatcatcg ccgtcgatac cattcagaag cgcaacctgc
      841 tcgaacggct gattgtcgat ttgcatatga accaggtcat cgtgttctgc aaaaccaaac
      901 aaagcgtcga ccgcgtaacg cgcgaactgg tgcgccgcaa cctgtccgca caggcgatac
      961 acggcgaccg ttcccaacaa agccggctcg aaacactcaa cgccttcaaa gacggcaacc
     1021 tgcgcgtcct cgtcgccacc gatatcgccg cgcgggggct ggatattgcc gaactaccct
     1081 tcgtcatcaa ttacgaaatg cccgcccagc ccgaagacta cgtccaccgc atcgggcgca
     1141 cggggcgcgc gggcgcggac ggcgtggcga tttccctgat ggacgaatcc gaacagaaaa
     1201 tgtttgaatc cattaaagag ctgaccggca acaagctgct catcgagcgc atcgagggct
     1261 tcgagccgca atggtgggaa cagggcggcg caaaaccgga aaaacccgaa atgcgcgaac
     1321 caagacaacg caaccgctac gaatccgcca aagcgcaacg cgaaaaaaac acccggccgg
     1381 aaaatgcggc aaacgatgcg ggcgcggctt gcggaaaaat tgccggacgc agccgccgaa
     1441 gccgccggga acaccggacg tgcgccctgc tccaaccgcg ttacggcgta aaatagccct
     1501 gaaaatcaaa tgccgtctga acatttcccg tttcagacgg catttttcaa actggactga
     1561 cgcatcggga gcaaccgccc gcaccggata aatttcttcc gcaaacagtt tcagacggca
     1621 tttgctgcct gtacaatatc atttcctttt ccactcaaac catcaggatt tcccaatgtc
     1681 cacccaattc gatgtcgccg taatcggcgc aggtccggca ggctcggtcg catccgccct
     1741 gctccgcaaa aaaggttata gtg
//
LOCUS       DAWHSK010000300         1731 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.300, whole genome shotgun sequence.
ACCESSION   DAWHSK010000300 DAWHSK010000000
VERSION     DAWHSK010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.300"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..106
                     /locus_tag="WIC03_002016"
     CDS             <1..106
                     /locus_tag="WIC03_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ3884467.1"
                     /translation="DRKSFPLFLKECKFRFNFGTPSRQLKILRDWCGI"
     gene            complement(277..792)
                     /locus_tag="WIC03_002017"
     CDS             complement(277..792)
                     /locus_tag="WIC03_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884468.1"
                     /translation="MSAFRKILLIISCLLIASCSFVETIFYMAISPEPVVVDFPLGKK
                     TKRSIELKQKIGKPYAISLGTNFIHYDPKQGERWIDDKLNYPYNISVKIFKVEEDGKK
                     LIIDELLTERSRKLGGGVFGAGGKYSMHIYDFYLPEGEYLFEISDNSEYIPLYDEINN
                     SIRIVVNARIQ"
     gene            complement(1053..1610)
                     /locus_tag="WIC03_002018"
     CDS             complement(1053..1610)
                     /locus_tag="WIC03_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FrpA"
                     /protein_id="HEZ3884469.1"
                     /translation="MENNKAKSDDNSVSIEFIQDFITASTDVINLSYENFRKNFYTQM
                     STDSTNYAAKHESLGKSVQRELQKTQSQLRQVVRKMQSKYNINNKARAAEISLLRQMQ
                     SQFSRKDVNKNLGNGNTLAQQGSYTKKDGTTAQAGDLLLAADNLHSRLTDKMLSISHV
                     RENTISPFVLGCLKQITINAYHYLK"
BASE COUNT          566 a          317 c          241 g          607 t
ORIGIN      
        1 tgatcgtaaa tctttcccgc tgttcttgaa agaatgcaaa tttcggttta acttcggcac
       61 accgtcccga cagctaaaaa ttttgcggga ttggtgtgga atttagggct aatctagtac
      121 agccccgatt tttttatctc tagccgaata cagttcaacc caaacgtacc cgtcttggat
      181 acaccccctc ttgtttcagt ggcgtaccct gcttcgcaaa ttttacagtt atcgcataat
      241 aatcagccat aagtaacccc acatttctag aaaaatttac tgaattcgtg cattaactac
      301 tattcttata gaattattta tttcatcgta aagtggaata tattcactat tatcagaaat
      361 ctcaaataaa tattcccctt ccggcaaata aaaatcataa atatgcatac tatatttccc
      421 cccagctcca aatactccgc ctcctaattt tctacttctc tctgtaagca actcatctat
      481 aataagtttt ttaccatctt cttccacttt aaatatttta accgatatat tatatggata
      541 gtttaactta tcatcaatcc acctctcccc ctgttttgga tcataatgta taaaattagt
      601 tcctaacgat attgcataag gtttaccaat tttctgtttg agttcaatag atctttttgt
      661 ttttttacca agaggaaagt ctaccacaac aggttctggg ctaatagcca tataaaaaat
      721 agtttcaaca aaactgcagc tagcaatcaa taggcaagat attattaata atatttttct
      781 aaatgcagac ataataaaaa tctccaaaaa atccatatca gaatatacta attatttgca
      841 taattaataa ggaagagaaa aattcaaaac ggctatgaga tttccgcctg aaaataatga
      901 aataaaagtc catagtaatg aataatagct ttatcagacc ccctttaatg caaacataga
      961 gaatacttcc cttgaagcac tatcaatttc tacttaacta aaactctagc tctatttcta
     1021 aaattttgtc aaaaatagtt aaatactttt atttatttta aataatgata tgcatttatg
     1081 gttatttgtt ttagacagcc taaaacaaac ggggaaatcg tgttttcccg aacatggcta
     1141 atggatagca ttttgtccgt gaggcggctg tgcaggttgt cagcagccaa cagcaaatcg
     1201 cctgcttgcg cggttgtgcc gtcttttttg gtgtagctgc cttgttgagc caaagtgttg
     1261 ccgttaccaa gatttttgtt tacatctttt cgagaaaatt ggctttgcat ttgccttaac
     1321 aaagatattt ctgctgctcg tgctttatta tttatattat atttactctg catttttctt
     1381 acaacttgtc tcaactgact ttgtgttttt tgtaattcac gttgtaccga ttttcctaaa
     1441 ctttcatgtt tggctgcata attggtagaa tcagttgaca tttgtgtata aaaattttta
     1501 cgaaaatttt cgtaactcag attaattaca tccgtacttg cagttataaa atcttgtata
     1561 aattcaatag aaactgaatt atcatcagat ttggctttgt tattttccat attaagcctc
     1621 tcttaatttg aaaaaattaa cataaacatt taaaaataaa acaaaattat ataataaaaa
     1681 aaataatgag aatataaagg ataaaaagag agttaagtta tttaatcgga c
//
LOCUS       DAWHSK010000301         1730 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.301, whole genome shotgun sequence.
ACCESSION   DAWHSK010000301 DAWHSK010000000
VERSION     DAWHSK010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.301"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            354..1658
                     /locus_tag="WIC03_002019"
     CDS             354..1658
                     /locus_tag="WIC03_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEZ3884470.1"
                     /translation="MKKHIWAASLLPASLSAEPLNWWKPYSAVNSGDTAWMMTAAALV
                     LLMTLPGLALFYGGMVRKKNLLSTMMHSFSIATLVGILWVAVGYSLAFTPGNAFIGGL
                     GRVFLSGMQIDAAAQMLTVSPNAPTVPEPVFMFFQMTFAIISTAIITGAFAERMKYSA
                     MMLFSGIWFLLVYVPGAHWVWGGGFMSKGGVLDYAGGTVVHINAGIAGLVAALVLGRR
                     IGYGREAMPPHNMAMTLIGAAMLWFGWFGFNAGSALAADAAAGMAMAVTQVSAVFGAA
                     GWLACEKIAGHKPSALGLASGAVSGLVGITPAAGFTGPSGAAAIGILTAAACFVSVTV
                     VKHKLRYDDSLDAFGIHGFGGLVGGILTGIFFDNRIFGGDAAVWQQLWIQVKDGVVMA
                     AYSGLMSWAILKVVGKICGGLRVGKDVEREGLDLNIHGERVE"
BASE COUNT          354 a          434 c          510 g          432 t
ORIGIN      
        1 ttaaaaattt ttaatatatt aatcaataaa ttaattttat aaaataaaaa tattgtcaac
       61 aatattttgc cttatcgccc aaacctctgt atattttcct acagtaaatt gttgacaatc
      121 catacgccca catatgcgcc gcctaaggat aaatcctccc gccggacaac gggtgcaagg
      181 gattggatgc gatatttcca tattcaaaca agggatttgt ttcacgcaca gggcggcaca
      241 tcggcaaaat ccccgcgccg ccccggtcca gcagggcttg cgtccctccc ggacaagccc
      301 cgaccccgcc tttccgaaag acgggctcaa ccattaagga aactttaatc aaaatgaaaa
      361 aacacatatg ggcggcatct ttgctgccgg catccctatc ggcagaacct ttaaactggt
      421 ggaagcctta ttccgccgtc aattcgggcg ataccgcctg gatgatgact gcggctgcct
      481 tggtactgtt gatgacgctt cccgggctgg ctttattcta cggcggtatg gtgcggaaaa
      541 aaaacctgct ctcgacgatg atgcacagct tttccatcgc gacattggtg ggcatccttt
      601 gggtcgccgt cggctattct ttagcgttca cgccgggaaa tgcctttatc ggcggtttgg
      661 ggcgcgtatt tttaagcggg atgcagatag acgctgccgc acagatgctg accgtgtcgc
      721 ccaatgcgcc gactgttccc gaaccggtat ttatgttttt tcagatgacg tttgccatta
      781 tttcgaccgc cattattacc ggcgcgtttg ccgaacggat gaaatattcg gcaatgatgc
      841 tgttttcggg catatggttt ttattggttt atgtgccggg cgcgcattgg gtgtggggcg
      901 gcggctttat gagcaagggc ggcgtattgg attatgccgg cggtacggtg gtgcacatca
      961 atgccggtat cgcgggactc gtcgccgcct tggttttggg caggcgcata ggctacgggc
     1021 gcgaggcgat gcctccgcac aatatggcga tgacactgat cggcgcggca atgttgtggt
     1081 tcggctggtt cggctttaac gccggatcgg cgcttgcggc agacgcggcg gcgggtatgg
     1141 cgatggcggt aacgcaggtg tcggccgtat tcggcgcggc aggctggctt gcctgcgaaa
     1201 aaatagcggg acacaaacct tccgctttgg ggctggcttc cggcgcggtt tccggtctgg
     1261 tcggcatcac ccctgccgcc ggctttaccg gcccgtcggg cgcggccgcc atcggtatat
     1321 tgactgccgc cgcgtgcttt gtgtccgtca ccgtcgtcaa acacaaattg cgttacgatg
     1381 attctttgga cgctttcggc atacacggat tcggcgggct ggtgggcgga atattgaccg
     1441 gcatcttttt cgacaaccgc attttcggcg gggatgcggc agtttggcag cagttgtgga
     1501 tacaggtaaa agacggggtc gttatggcgg catacagcgg gctaatgagt tgggcgattt
     1561 tgaaggtcgt ggggaaaatc tgcggcggcc tgcgcgtcgg caaggatgtc gaacgcgaag
     1621 gtttggatct gaatatccac ggcgaacgcg tggaataagg gcggctatgc cgtctgaagc
     1681 ctgaaaatcg ggtttcagac ggcatttttc acgtttgccg ccgatggata
//
LOCUS       DAWHSK010000302         1712 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.302, whole genome shotgun sequence.
ACCESSION   DAWHSK010000302 DAWHSK010000000
VERSION     DAWHSK010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.302"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            23..451
                     /locus_tag="WIC03_002020"
     CDS             23..451
                     /locus_tag="WIC03_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ3884471.1"
                     /translation="MGNFAKVSGYTYNKSRYRNAAEVNAERLAKNSSADPYNFSNFTP
                     VHIFRFGTSFHIPNTGLTVGGGVSAQSSTSSLYNIRQGGYGLIDGFVRYELGKHAKLS
                     LIGTNLNGRTYFENNYNRTRGANNFYGEPRTVSMKLDWQF"
     gene            594..1652
                     /gene="dinB"
                     /locus_tag="WIC03_002021"
     CDS             594..1652
                     /gene="dinB"
                     /locus_tag="WIC03_002021"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEZ3884472.1"
                     /translation="MSSRKIIHIDMDAFYASVELREQPHLKGRPVVVAWEGARSVICA
                     ASYEARQFGLHSAMSVATAKRLCPQAVYVPPHFDLYRQVSAQIHAVFRRYTDLIEPLS
                     LDEAYLDVTRNFKNIPYASEVAKEIRAAIFAETGLTASAGIAPNKFLAKIASDWRKPN
                     GQFVLPPHKVMAFLETLPLGKIPGVGKVTLKKMQSLGMRTAGDLRRFERGELLNHFGR
                     YGYRLYDLVRGTDERPVKAERERLQISTEITLPEDLPLEQAAGHLPHLAEDLWRQITR
                     KNVEAQSVTLKLKTYDFRIITRTLTYSSVLPDCAALLQAAQMLMARVPPQTEDAFRLI
                     GIGVGHLVPKNQQQDLWA"
BASE COUNT          405 a          466 c          469 g          372 t
ORIGIN      
        1 cttggtggga atttagggga ttttggggaa ttttgcaaag gtctcaggtt atacctacaa
       61 caagagcagg tataggaatg cagccgaagt caacgccgaa cgccttgcca aaaattccag
      121 tgcagacccg tacaacttca gcaatttcac acccgtgcat atattccgtt tcggaacgag
      181 tttccatata ccgaatacgg ggctgaccgt cggcggcggc gtgtccgcac aaagcagcac
      241 aagcagcctg tacaacatca ggcagggcgg ctacgggctg atagacggtt tcgtccgtta
      301 cgaattgggc aaacacgcca aattgagcct catcggtacg aacttaaacg gacgcactta
      361 ttttgagaac aactacaacc gtacgcgcgg cgcaaacaac ttctatggag agccgcgcac
      421 tgtcagcatg aaactggatt ggcagtttta attgaaaacc gtagttttgc ggatgccgtc
      481 tgaacagggt atgtttcaga cggcattttt atggaggcag gcctatcggg gcgtatattt
      541 ggaatttgct cgatgccggc agtagaatat cctttttatc ttcagcagag ccgatgtctt
      601 cacgcaaaat catccacatc gacatggacg cgttctacgc atcggtagag ctgcgcgaac
      661 agccgcattt gaaagggcgg ccggtggtcg tcgcgtggga gggcgcgcgt tcggtgattt
      721 gcgccgcatc gtatgaggca cggcagttcg ggctgcattc cgcgatgtcg gtggcaacgg
      781 cgaaaaggct gtgtccgcaa gcggtgtatg tgccgccgca tttcgatttg taccgtcagg
      841 tttccgcgca gattcacgcc gtattcaggc gttataccga tttaatcgaa cccttgtcgc
      901 tggacgaagc ctatcttgac gttacccgta atttcaaaaa catcccttac gccagtgaag
      961 ttgccaaaga aatccgtgcc gccatttttg cggaaacagg tttgactgca tccgcaggca
     1021 tcgcgccgaa caaatttctg gcgaaaatcg cgtcggactg gcgcaagccg aacgggcagt
     1081 ttgtgttgcc gccgcacaaa gtcatggcat ttttggaaac cctgcctttg ggcaaaatcc
     1141 ccggcgtggg caaggtaacg ctgaaaaaaa tgcagtcgct gggtatgcgg acggcgggcg
     1201 acttgcgccg tttcgagcgc ggcgaactct taaaccattt cggacgctac ggataccgcc
     1261 tctatgattt ggtgcgcggt acggacgaac gccccgtcaa agccgaacgc gaacgcctcc
     1321 aaatctccac agaaattacc ctgcccgaag acctgccgct cgagcaggct gccggacacc
     1381 tcccccatct tgccgaagac ttgtggcggc aaatcacgcg caaaaacgtc gaagcccaaa
     1441 gcgtaacgct caagctgaag acctacgatt tccgcatcat cacgcgcaca ctgacttatt
     1501 cctccgtatt gcccgactgc gcagctctgc tgcaggctgc gcaaatgttg atggcgcgcg
     1561 tcccgccgca gacggaagac gcgttccgcc ttatcggtat cggcgtgggg catcttgtgc
     1621 cgaaaaacca gcagcaggat ttgtgggcgt aaaccgcttt acgcgcgccg tccaaaaaat
     1681 atgccgtctg aacggttttc agacggcatg gc
//
LOCUS       DAWHSK010000303         1681 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.303, whole genome shotgun sequence.
ACCESSION   DAWHSK010000303 DAWHSK010000000
VERSION     DAWHSK010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.303"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..>1681
                     /locus_tag="WIC03_002022"
     CDS             <1..>1681
                     /locus_tag="WIC03_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HEZ3884473.1"
                     /translation="KERPFVLENIGYSEVGAARAESRLSPERPAVQVRWLHENDNEKA
                     NKDVLRRRAADYCADEIAHALNEAAGGRLNFKGCPLQSGDIAVLVRTHNEAVMVSAAL
                     KKRQVQSVLLSRESVFASPEAAALSALIGFWLEPRRAGTLRFVLTSGIFGYDAQQLHD
                     FNQNESEILHWAESARTALDNWNQYGIFAAMQQFSQTHGIETRLLSRNNGRSLTNYFQ
                     LLELLAAEDAQNRNPAALHKWLRDQISLAGNNSGDNRAIRLESDEDLVKIVTMHASKG
                     LQYPLVYCPFAWDAQDTGPSDWQILHQSANRTELLAKAQLSEDEQKQYADEEMAERLR
                     LLYVALTRAEEQLNIYAAYSTNTADNPLAYLIEGSPQDSRETVRRAYACEKDGIAMLK
                     RNWRRVADNAPSGTNFAFTENAPPPAAYRSNAGQTAEFAANSIPERGFRFVRHTSFTA
                     LSRHTQTPDGGEEDACPSLDAAETSVPAMPSETPTASDGISIHDFPKGTQAGLCLHEI
                     LEDFKFGQAAAGQETLIADKLKKYGFEEIWLPAVAEMAEACRKTPLTGAYDL"
BASE COUNT          408 a          542 c          444 g          287 t
ORIGIN      
        1 caaagaacgc ccgttcgttt tggaaaacat cggctattcg gaagtcggtg cggcgcgtgc
       61 cgaaagcagg ttgtcccccg aacgtcctgc cgtacaggtt cgttggctgc acgaaaacga
      121 caatgaaaaa gccaacaaag acgttttgcg ccgccgtgcc gccgactatt gcgccgacga
      181 aatcgcccac gcgctcaacg aagccgccgg aggccgtctg aatttcaaag gctgcccgtt
      241 gcagtcgggc gatattgccg tgctggtacg cacgcacaac gaggcggtga tggtttccgc
      301 cgccctgaaa aaacggcagg tgcaaagcgt cctgctttcg cgcgaatctg tgttcgcctc
      361 gcccgaagcc gccgccctgt ccgcactcat cggcttctgg ctcgaaccgc gccgcgccgg
      421 aacgctgcgc tttgtcctga caagcggcat attcggttat gacgcgcagc aattgcacga
      481 cttcaaccaa aacgaaagcg agattttgca ttgggcggaa tctgcccgaa ccgcactcga
      541 caactggaat caatacggca ttttcgccgc tatgcagcaa ttttcccaaa cacacggcat
      601 cgaaacgcgc ctcttaagcc ggaacaacgg gcgcagcctg accaactatt tccaactgct
      661 cgaactgctt gccgccgaag acgcgcaaaa ccgcaaccca gccgcgctgc acaaatggtt
      721 gcgcgaccaa atcagcctgg ccggcaacaa cagcggcgac aaccgcgcca tccgtctgga
      781 aagcgacgaa gatttggtca aaatcgttac catgcacgcc tcgaaaggtt tgcagtatcc
      841 gctggtgtac tgcccgtttg cgtgggacgc gcaagatacc gggccgtccg actggcaaat
      901 cctccaccaa agcgcaaacc gaaccgaact gttggcaaag gcgcaactgt cggaagacga
      961 acagaaacaa tacgccgatg aagaaatggc ggaacgcctg cgcctgcttt atgtcgcact
     1021 gacgcgtgcc gaggaacagc tcaacatcta cgccgcgtac tccaccaata ctgccgacaa
     1081 ccccctcgcc tacctgattg aaggctcgcc ccaagacagc cgtgaaaccg tccgccgtgc
     1141 ctatgcgtgt gaaaaagacg gcatcgcgat gctcaaacgc aactggcggc gcgtggcgga
     1201 caacgcccct tccggcacaa atttcgcctt caccgaaaat gcgccgcccc ctgccgccta
     1261 tcgcagcaac gccggtcaaa ccgccgaatt tgccgcaaac agcattcccg aacgcggatt
     1321 ccgatttgtc cgccacacta gctttaccgc cttaagccgc catacccaaa cgcccgacgg
     1381 cggcgaagaa gatgcctgcc cgtccttgga tgccgccgaa acctcggtgc cggcgatgcc
     1441 gtctgaaacg ccgacggctt cagacggcat atcgatacac gattttccga aaggcacgca
     1501 ggcggggctg tgcctgcacg aaattcttga agatttcaaa ttcggacaag cagccgccgg
     1561 acaggaaacc ctcattgccg acaagctgaa aaaatacggt tttgaagaaa tatggctgcc
     1621 cgccgttgcc gaaatggcgg aagcctgccg caaaacgccg ctgacggggg catacgacct
     1681 g
//
LOCUS       DAWHSK010000304         1663 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.304, whole genome shotgun sequence.
ACCESSION   DAWHSK010000304 DAWHSK010000000
VERSION     DAWHSK010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.304"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(37..699)
                     /locus_tag="WIC03_002023"
     CDS             complement(37..699)
                     /locus_tag="WIC03_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ3884474.1"
                     /translation="MIHAVLFDLDGTLADTALDLGGALNTLLARHGLPAKSMDEIRTQ
                     ASHGAAGLIKLGAGITPDHPDYARWRTEYLEEYDRRYAQDTELFDGVNELIAELNQRG
                     IKWGIITNKPMRFTDKLVPKLGFIIPPAVVVSGDTCGEPKPSIKPMLYACGQIHADPQ
                     HTLYVGDAERDIQAGRNAGMKTVLAEWGYISDEDDTDSWQADFHIRTPLDLLECLDKI
                     QP"
     gene            complement(712..1539)
                     /locus_tag="WIC03_002024"
     CDS             complement(712..1539)
                     /locus_tag="WIC03_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEZ3884475.1"
                     /translation="MKLDIYALLERALLSGDPDEKGRLTDEAFAAVQNADGAEANAPP
                     ADFPRAGRPDKPVLVAPSQLTPRKMNTTEGYAAMLHAIAHIEFNAVNLALDAAYRFRT
                     LPFQFVRDWVKVAKEEVYHFRLMRDRLRAFGFDYGSFEAHNHLWDMAYKTAYDPLLRM
                     ALVPRVLEARGLDVTPGIRAKVAQRGDSETCGVLDIIYRDEVGHVAIGNHWYQHLCRE
                     RGLEPITLFRSLIARYDMFIFRGYVNIEAREKAGFSRFELDMLEDFEQGLKQNKNTV"
BASE COUNT          323 a          438 c          501 g          401 t
ORIGIN      
        1 atttaatcca ctatatgttt gtggggcgga tatttttcag ggctgtattt tgtctagaca
       61 ttcgagcaga tcgagcggcg ttcggatgtg gaaatccgcc tgccatgagt cggtatcgtc
      121 ttcatctgag atgtagcccc attcggcgag gacggttttc atgccggcgt tgcggcctgc
      181 ttggatgtcg cgttcggcat caccgacgta gagtgtgtgt tgcgggtcgg cgtggatttg
      241 tccgcacgca tacagcatgg gtttgatgct gggcttgggt tcgccgcagg tgtcgccgct
      301 gacgacgacg gcgggtggga tgatgaagcc gagtttgggg acgagtttgt cggtgaagcg
      361 catgggtttg ttggtgatga tgccccattt gatgccgcgt tggttgagtt cggcaatgag
      421 ttcgttcacg ccgtcaaaga gctcggtgtc ttgggcgtag cggcggtcgt attcctccag
      481 atattcggtg cgccatcggg catagtcggg gtggtcgggg gtgatgcctg cgccgagttt
      541 gatcagtcct gccgcgccgt ggctggcttg ggtgcggatc tcgtccatgc tttttgcagg
      601 tagtccgtgg cgagcgagca gggtgttgag tgcgcctccg aggtcgaggg cggtgtcggc
      661 aagtgtgccg tcgaggtcga agagtacggc gtgtatcatg tgtgttcctt ttcagacggt
      721 atttttgttc tgtttcaaac cctgctcgaa atcttccagc atatccaatt caaagcggct
      781 gaagcccgct ttttcgcgcg cttcgatgtt cacatagccg cggaagataa acatatcgta
      841 acgggcaatc agactgcgga acagggtaat cggttccaaa ccgcgttcgc ggcagaggtg
      901 ttgataccaa tggttgccga tggcgacatg gcccacttcg tcgcggtaaa tgatgtccaa
      961 tacgccgcag gtttccgaat cgccgcgctg cgccaccttc gcgcgtatcc ccggcgtaac
     1021 gtccagcccg cgcgcttcca acacgcgcgg taccaacgcc atacgcaaca gcgggtcgta
     1081 ggcggttttg tacgccatat cccacaaatg gttgtgcgcc tcgaaactgc cgtaatcgaa
     1141 gccgaaagcg cgcaatctgt cgcgcatcag gcggaaatgg tacacctctt ccttcgccac
     1201 tttcacccag tcgcggacaa actgaaacgg cagcgtgcgg aaacggtatg ccgcgtccaa
     1261 agctagattg acggcgttga attcgatatg cgcaatcgcg tgcagcatcg ccgcataacc
     1321 ttcggttgtg ttcattttgc gcggggtcag ttgcgacggc gcgaccaaaa caggcttgtc
     1381 cggccgtccc gctcggggga agtccgccgg cggcgcgttt gcttccgccc cgtccgcatt
     1441 ttgaacggcg gcaaacgcct catccgtcag ccgtcctttt tcatctgggt cgcccgaaag
     1501 cagggcgcgt tccagcaaag cataaatatc gagtttcatc tcaagtccgc cgtgttcgga
     1561 aaacaaatat tatagcgttt taaaaaaaca agatgaggca tataatctcc gcgattcggc
     1621 attccgcgcc caaaccgtca aatatagtgg attaacaaaa acc
//
LOCUS       DAWHSK010000305         1649 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.305, whole genome shotgun sequence.
ACCESSION   DAWHSK010000305 DAWHSK010000000
VERSION     DAWHSK010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.305"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(287..973)
                     /locus_tag="WIC03_002025"
     CDS             complement(287..973)
                     /locus_tag="WIC03_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HEZ3884476.1"
                     /translation="MYAFTDAQQQKALFRLVLFHILIIAASNYLVQFPFQIFGIHTTW
                     GAFSFPFIFLATDLTVRIFGSHLARRIIFWVMFPALLLSYVFSVLFHNGSWTGLGALS
                     EFNTFVGRIALASFAAYALGQILDIFVFNKLRRLKAWWIAPTASTVIGNALDTLVFFA
                     VAFYASSDGFMAANWQGIALVDYLFKLTVCGLFFLPAYGVILNLLTKKLTTLQTKQAQ
                     DRPAPSLQNP"
     gene            complement(1049..1522)
                     /gene="queF"
                     /locus_tag="WIC03_002026"
     CDS             complement(1049..1522)
                     /gene="queF"
                     /locus_tag="WIC03_002026"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HEZ3884477.1"
                     /translation="MSRNTEELQGISLLGNQKTQYPIGYAPEILEAFDNKHPDNDYFV
                     KFVCPEFTSLCPMTGQPDFATIYIRYIPHIKMVESKSLKLYLFSFRNHGDFHEDCVNI
                     IMKDLIALMDPKYIEVFGEFTPRGGIAIHPFANYGKAGTEFEALARKRLFEHDSQ"
     regulatory      complement(1526..1570)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
BASE COUNT          421 a          361 c          497 g          370 t
ORIGIN      
        1 atacagtcga ttaaaataaa actaagccaa ggaagcactg ccgtcattcc cgtaccgtcg
       61 ggaatccgga caccacggta cggaaaccca tccgccgtca ttcccgcgaa agcgggaatc
      121 tagaaataca atgcggcagg agtttatcgg aaatgactga aacccaacgc accggattcc
      181 cgctttcgcg ggaatgacga agtgggcggg aatccggatt tatccgttcc gacagtgttt
      241 gcaaataaaa gaaaacccaa ccgtcccgat ttccggcagg gctgttttac ggattttgca
      301 gcgagggcgc ggggcggtct tgcgcctgtt tggtttgcag ggtcgtcagt tttttcgtca
      361 gcagattcag aatcacgccg taggcgggca ggaaaaacag accgcagacg gtgagtttga
      421 acaggtaatc gaccaaggcg atgccctgcc agtttgccgc cataaatcca tcgctgctgg
      481 cgtaaaaggc aacggcgaaa aataccagcg tatccaaagc gttgccgatg acggttgatg
      541 cggtcggggc aatccaccac gctttcagac ggcgtaattt gttgaacaca aaaatatcaa
      601 ggatttgtcc gagcgcgtag gcggcaaagc tggccagcgc gatgcgtccg acaaaggtgt
      661 tgaattcgga cagtgcgccc aagcccgtcc aactgccgtt gtggaacaaa acggaaaaga
      721 cataggaaag caaaagggcg gggaacatga cccaaaagat aatccgccgt gccaagtgcg
      781 aaccgaaaat gcggacggtc aggtcggtgg caaggaagat gaagggaaag gaaaacgcgc
      841 cccaagtggt gtggatgccg aaaatttgga aggggaactg caccagatag ttgctggcgg
      901 cgatgatgag gatatgaaaa agcaccagcc ggaagagtgc cttctgttgc tgtgcgtcgg
      961 taaatgcgta cataaaaaaa cctttcggaa aggcgttcag acggcatacc gtatcgaaag
     1021 aatgccgtct gaaagtggga aggacggatt attggctgtc gtgttcgaac aagcgtttac
     1081 gcgccaatgc ttcaaactcg gtgcctgctt tgccgtagtt ggcaaacgga tgaatggcga
     1141 tgccgccgcg cggtgtgaac tcgccgaata cttcgatgta tttcggatcc atcagggcaa
     1201 tgaggtcttt catgatgatg ttgacacagt cttcatgaaa atcgccgtgg ttgcggaagc
     1261 tgaagaggta gagtttcagg gatttgcttt ccaccatttt gatgtgcgga atatagcgga
     1321 tgtagatggt ggcgaagtcg ggctgcccgg tcatggggca gaggctggtg aactctgggc
     1381 agacgaattt gacgaaatag tcgttgtcgg gatgtttgtt gtcgaatgct tcgagaattt
     1441 caggcgcgta gccgatcgga tattgggttt tttgattacc caaaagcgag atgccttgca
     1501 gctcttcggt attgcgggac atagggtttc cttagttttt tagtgtggga ggttttcgaa
     1561 ccacggggcg gcgattgtaa tataagcggc ggtatctgtg tagttttctt cagacggcat
     1621 ggtttggacg gcggcgtttt ccgtgtcat
//
LOCUS       DAWHSK010000306         1635 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.306, whole genome shotgun sequence.
ACCESSION   DAWHSK010000306 DAWHSK010000000
VERSION     DAWHSK010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.306"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(124..336)
                     /locus_tag="WIC03_002027"
     CDS             complement(124..336)
                     /locus_tag="WIC03_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ3884478.1"
                     /translation="MELGYTPYNLRTLRNRCKLTQAELAQIVGVKHYIQVGRWEAEPD
                     TETRRADMPLEKWRQFLDWTEKTNAV"
     gene            complement(348..629)
                     /locus_tag="WIC03_002028"
     CDS             complement(348..629)
                     /locus_tag="WIC03_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884479.1"
                     /translation="MNRAALFNRYPEWIIGQDGASRFITHCRYPRLIAKIHRQKDGEC
                     PGGHYRHSENGITLYDFIFFDGKPADEARFAAVLTETCRRAVKKICETK"
     gene            complement(666..929)
                     /locus_tag="WIC03_002029"
     CDS             complement(666..929)
                     /locus_tag="WIC03_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884480.1"
                     /translation="MADILPSDKRRQPPKTKGNPMNIIEQETKRAELRKIDAEIAKII
                     ADAHKINAESVKIAQESRWYPMMAATGLVTAIAAVLALIFKFA"
     gene            complement(1023..1574)
                     /locus_tag="WIC03_002030"
                     /pseudo
     CDS             complement(1023..1574)
                     /locus_tag="WIC03_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273911.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
BASE COUNT          242 a          361 c          566 g          466 t
ORIGIN      
        1 gtgagtttat tttggggcgg cggcaggtcg gggcaagcgg cgtggggctt ggttgtggtt
       61 tttaggtttg ggggtgggta aaaaatgccg tacccggcgg gcaggcttcg cactctgaag
      121 gtttcagacg gcgtttgttt tttctgtcca atcgaggaac tgccgccatt tttccagcgg
      181 catatcggcc cgccgcgttt cggtatcggg ttcggcttcc cagcggccta cctgtatgta
      241 gtgcttcacc ccgacgattt gcgccaactc ggcctgtgtc agtttgcagc ggttgcgaag
      301 ggtgcgaagg ttgtaaggcg tgtagccgag ttccatgtcg ttgcggttta ttttgtttcg
      361 catatttttt tgactgccct gcggcaggtt tcggtaagga cggcggcaaa tcgggcttcg
      421 tctgccggtt ttccgtcgaa aaaaataaaa tcgtaaaggg tgatgccgtt ttcggaatgg
      481 cggtaatgcc cgcccgggca ttcgccgtct ttctgcctgt ggattttggc aatcaggcgg
      541 ggatagcggc aatgggtaat gaaacggctt gcgccgtctt gcccgataat ccattcgggg
      601 tagcggttga atagtgcggc tctgttcatt ttgttgaggg ataaagcccc ttgcggggct
      661 tgtggtcagg caaatttgaa tatcagtgcc aacacggcgg cgatggcggt aaccagaccg
      721 gtggccgcca tcatgggata ccagcgggac tcttgggcta tttttacgga ttcggcgttg
      781 attttgtgcg cgtcggcaat gattttggcg atttcggcat ctatttttct taactcggcg
      841 cgtttcgttt cttgttcgat tatgttcatt ggatttcctt tcgtttttgg cggttgccgc
      901 cgcttgtcgg atggcaggat gtctgccatg tatgtatatt aatacctttt aggttttatt
      961 gcaagtgttt tgggcaggat gccgtctgaa tttttttcag gcggtttttt gttttgcggg
     1021 gttttggcgg cggcttcgta tgcttggcgg tggcggcggc tgtaccattc ggcgagttcg
     1081 cgccgctctt ggaggcggta gctgtcggcg gtcaggtagt ggtgcaggct tgagaagccg
     1141 gcgcgttgga gggcggcgcg gctgatgtgg ccgagggcga ggtctgttgt gagggtgtct
     1201 tttccggcgg tcaggctgat gttggtgaag aggtggcgga atccgtgcat tgtgtgtttg
     1261 gatttgccgg gagtgctgcc gtcatagccc agtcgccgga tggcgttgtg ggcgaatttg
     1321 atgctgatgt ggtcgggatg gggggcgggt ttgcgccgtg ggcggatgcc tgggaaaagg
     1381 tggatgttgt cgccggtctg tgtgtgcagc tctcggagta tttctaccgc ccagtccgac
     1441 agtgggacgg taaaggggtg tttggtcttc atgtcggcgg cggggatgtg ccataaccgg
     1501 gcggtgaggt cgatgtcttg ccagcgggcg gaaagcagtg cggagggacg ggatgacttc
     1561 cccggtttcc ccatcaatcc gcgccgcctc gatttttccg ttggcgcgca gcatttccac
     1621 cgcctgccgc accga
//
LOCUS       DAWHSK010000307         1634 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.307, whole genome shotgun sequence.
ACCESSION   DAWHSK010000307 DAWHSK010000000
VERSION     DAWHSK010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.307"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(169..>1634)
                     /gene="recJ"
                     /locus_tag="WIC03_002031"
     CDS             complement(169..>1634)
                     /gene="recJ"
                     /locus_tag="WIC03_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEZ3884481.1"
                     /translation="DYDADGATACAVGLDGLAAMGAKVDFLVPNRFEHGYGLTPELAE
                     IAAAQGVDLLITVDNGIASIAGVARAQELGLDVIVTDHHLPAETVPDCIIVNPNQKGC
                     GFQSKSLAGVGVIFYVLMALRAELRRRNYFSDGLKEPNLGDLLDLVALGTVADVVSLD
                     HNNRILVSQGLKRMRSGKMRPGIRALFEVARRDWRKAQPFDMGFALGPRINAAGRLDD
                     MSVGIACLLVRDDVEAQELAAQLNNLNIERREIEQSMLQDALNAFPETLPSGQTTLVA
                     YRDDFHQGVVGIVASRLKDRFYRPTIVFAPADNGEVRGSGRSIPNLHLRDALDLVSKR
                     HPDLILKFGGHAMAAGLSILEHNIPAFQTSFEEAVREMVCEDDLSQTFITDGSLPACD
                     ITLEQAQNLARHVWGQGFAPPSFTDEFHVVRQQPLGAEGKHKKVWLQKDGCEFEAMFW
                     RCSEDIPEYIRTVYRPVANEWRNNLELQLYIDYWEAV"
BASE COUNT          380 a          472 c          436 g          346 t
ORIGIN      
        1 aatccgccgg agtctgaaat ttgatgcacc ttatatattg cgtccctaag aagggatgat
       61 taacaaaaat taacgccctt tactttcttt tccgattggg aacattcccg tcgtacaaac
      121 aggcaaatga aggaacagaa atcacattca aacggcgttc cgccgcctct acaccgcttc
      181 ccagtaatcg atatacagct gcaattcgag attgttgcgc cattcgttgg caacggggcg
      241 gtaaaccgtg cggatgtatt cggggatgtc ttcgctgcaa cgccaaaaca tggcttcaaa
      301 ttcgcagccg tctttttgca gccagacttt tttgtgtttg ccctccgcgc ccaaaggttg
      361 ctggcggacg acgtggaact cgtcggtaaa gctcggcggc gcgaagccct gcccccaaac
      421 gtgacgggca aggttttgcg cctgttccaa cgtgatgtcg caggcgggca ggctgccgtc
      481 ggtgatgaag gtttgcgaca aatcgtcttc gcacaccatt tcgcgcacgg cttcttcaaa
      541 ggacgtctga aacgcgggaa tgttgtgttc aagtatgctc aaacccgccg ccatcgcgtg
      601 tccgccgaat ttcaaaatca aatcgggatg gcgtttggac accaagtcca aagcatcgcg
      661 caggtgcaaa ttgggaatgg aacgtcccga accgcgtact tcgccgttgt cggcaggcgc
      721 aaacacgatg gtcggacgat aaaaacggtc tttgaggcgg ctggcgacaa tgccgaccac
      781 accttgatgg aagtcgtcgc gatacgccac caaagtcgtc tgacctgaag gcagggtttc
      841 aggaaaagca ttcagcgcgt cttgcagcat agactgctcg atttcgcgac gctcgatatt
      901 gaggttgttt aactgagccg ccagttcctg cgcttccaca tcatctcgca ccaacagaca
      961 ggcaatgccg accgacatat cgtccaaccg tccggcggca ttgatgcgcg gccccaacgc
     1021 aaagcccata tcaaacggct gcgctttgcg ccagtcgcgc cgcgccactt caaacaaggc
     1081 gcggataccg gggcgcattt tgcccgaacg catccgtttc aaaccttgcg acacgaggat
     1141 acggttgttg tgatcgaggg agacgacgtc ggcaacggta ccgagtgcga ccaaatccaa
     1201 aaggtcgccc agattcggct cttttaggcc gtctgaaaaa taattgcggc ggcgcagttc
     1261 ggcacgcaaa gccatcagta cataaaaaat cacgcccacg cccgccaagc ttttgctttg
     1321 gaaaccgcag cctttttggt tcggattgac gatgatgcag tctggcacgg tctcggcagg
     1381 cagatggtgg tcggttacaa tcacatccaa acccaattcc tgcgcacgcg ccacgcctgc
     1441 gatgctggca atgccgttat cgaccgtaat cagcaaatcc acgccttgcg cggcggcgat
     1501 ttcggccagc tcgggcgtta aaccgtagcc gtgttcaaag cggttgggca cgaggaaatc
     1561 tacttttgcc cccatcgccg ccaaaccgtc caaaccgacg gcacacgcgg tcgcgccgtc
     1621 ggcatcgtag tcgg
//
LOCUS       DAWHSK010000308         1633 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.308, whole genome shotgun sequence.
ACCESSION   DAWHSK010000308 DAWHSK010000000
VERSION     DAWHSK010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.308"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            196..408
                     /locus_tag="WIC03_002032"
     CDS             196..408
                     /locus_tag="WIC03_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEZ3884482.1"
                     /translation="METLILDIGGMSCGGCVKSVTRILEGVKGVASVEVSLENKSATV
                     GYDPAQTDAGALIEAVEDGGYDAALK"
     gene            556..>1633
                     /locus_tag="WIC03_002033"
     CDS             556..>1633
                     /locus_tag="WIC03_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase regulatory domain-containing
                     protein"
                     /protein_id="HEZ3884483.1"
                     /translation="MPNKKKKAAFARAATENGVPDVSNIKNSYDDLMYESKAFSQTAI
                     NALEARARMMGLQPAPAANAKVLELGCSMGGNIITQALYYPDAEFVGIDLSGRQVAQG
                     NAIIEKMGLKNVRLEEKDILTVDESFGKFDYIIVHGIWSWVPDAVKDKIFSICRNNLT
                     EQGIAYISYNVYPGWKRQEQLREIMYFAGRDVLEEPLEARTRKGLDALKALAEILEND
                     KGLDGGGKLPAIQKILNHNFYYIAHEYMEAFNDPIYVNGFIEWANRHRLAYIGDTDLH
                     ASFVSWMAEHTRERILALAGGDYIAKEFYSDILSDRQFRRSLLCREEVGDTVRRDESV
                     AVEVIESLNFRPARGETIDFDENDT"
BASE COUNT          415 a          388 c          452 g          378 t
ORIGIN      
        1 ccgattgcca aggcgatgat gctcaaccag ggtttttgcg aagcgttcat tgttgtcgtc
       61 ctgatgggaa ttgccgccat tatgcgcaag cgtccgtctt tttccaaacc gccgcaccgt
      121 ttccgcgttt gcccaagttc acgccccgac ttacaataac gcccgtcaga ttgcagttcc
      181 aacgaaagga aaatgatgga aacccttatc ctcgacatcg gcggcatgag ctgcggcggc
      241 tgcgtcaaaa gcgttacccg gatattggaa ggcgtaaaag gcgtggcaag cgtcgaagtc
      301 agtcttgaaa acaaaagtgc gaccgtcgga tacgaccccg cgcaaaccga tgcaggggcg
      361 ttgattgaag ccgttgaaga cggcggctat gatgcggcgt tgaaataaag cggcaaaaat
      421 gccgtctgaa gccttcgcgc ctccccgatg ccgtctgaag tacgctccgc gccttcagat
      481 ggcatttgat ttagttgaac gggtaggatg aattggttaa acttgcgggc aatagtgcct
      541 tcagacaaac aaaatatgcc gaacaaaaag aaaaaagccg cctttgcgcg tgctgcaacg
      601 gagaacggtg tgccggacgt gtccaacatc aaaaattcat atgatgattt gatgtatgaa
      661 tccaaagcat tttcccaaac cgccatcaac gccttggagg ctcgcgcccg catgatgggt
      721 ttgcagcccg cgcccgccgc caatgccaag gtcttggagc tgggctgttc gatgggcggc
      781 aacatcatca cgcaggctct ttattacccg gatgcggaat ttgtcggtat cgacttgtcc
      841 ggcaggcagg ttgcgcaggg caacgccatc attgaaaaaa tgggcttgaa aaatgtgcgt
      901 ctggaagaaa aagatatttt gaccgtcgat gagtcattcg ggaagtttga ctatatcatc
      961 gtccacggca tttggtcgtg ggtgcctgac gcagttaaag acaaaatctt ttcgatttgc
     1021 cggaacaacc tgaccgaaca gggcattgcc tatatttcat acaatgttta cccgggctgg
     1081 aagcggcagg agcagttgcg cgaaattatg tactttgccg gcagggatgt gcttgaagaa
     1141 cccttggaag cgcgaacgcg gaaaggcttg gacgcgctca aggcgctggc ggaaatttta
     1201 gaaaacgaca agggcttgga cggcggcggc aaacttccgg cgattcaaaa aatattgaat
     1261 cataattttt attacatcgc acacgaatat atggaggcgt ttaacgaccc gatttacgtc
     1321 aacggcttca tcgaatgggc taatcgccac aggctcgcat atatcgggga taccgatttg
     1381 cacgcgtctt ttgtttcttg gatggcggag catacgcggg agcggattct ggcattggca
     1441 gggggcgatt atattgccaa agaattttac agcgatattt tatccgaccg ccaattccgc
     1501 cgttcgcttt tatgccgtga ggaagtcggg gatactgtca ggcgtgatga gtcggttgcc
     1561 gtcgaagtga tagaaagttt gaatttccgc ccggcaagag gggaaacgat cgactttgat
     1621 gaaaacgata ccc
//
LOCUS       DAWHSK010000309         1599 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.309, whole genome shotgun sequence.
ACCESSION   DAWHSK010000309 DAWHSK010000000
VERSION     DAWHSK010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.309"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            176..781
                     /locus_tag="WIC03_002034"
     CDS             176..781
                     /locus_tag="WIC03_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEZ3884484.1"
                     /translation="MNTDFQNILKQLGKQAQKEAAEKQAAEKNKQKQEQDFDFSQAVG
                     QVSPLKNRQQYYAPPDKTPIKVRPKDNRADEENYFYIGSTYNDPPASFSKNGQGKNDI
                     QRLKNGYYPVVTDVDLHGYTQEEAQKVLNEFIAFTQKRGVCGEIIHGSGLGSKGYKPV
                     LKNMTRNWLMQHPDVLAYAEPREGNDGCVRILLKRKLRQQD"
     gene            844..1206
                     /gene="ssrA"
                     /locus_tag="WIC03_002035"
     tmRNA           844..1206
                     /gene="ssrA"
                     /locus_tag="WIC03_002035"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT          512 a          427 c          321 g          339 t
ORIGIN      
        1 ggtccgcatt tccggcaatc aacactgccg acggttgccg catttctttt tgatatacaa
       61 tcacactttc aaaaaacata cgcaggcaac aaaatcatcc tcccccgatt ggcaaacatt
      121 ccggcaaatt aaaaccccgc ccgaaacagc catcggacaa ataacaggaa aaaatatgaa
      181 taccgacttc caaaacatcc tcaaacaact gggcaaacaa gcccaaaaag aagcggcaga
      241 aaaacaggca gccgaaaaaa acaagcaaaa acaagaacag gatttcgatt tttcacaagc
      301 agtcggacaa gtctccccat taaaaaaccg gcagcaatat tatgcgccgc ccgacaaaac
      361 ccccatcaaa gtccgtccca aagacaaccg cgccgacgaa gaaaactact tctacatcgg
      421 cagcacatac aacgacccgc ccgccagctt cagcaaaaac ggacagggaa aaaacgacat
      481 ccaacgcctg aaaaacggat actatcccgt cgttaccgat gtcgacctgc acggctacac
      541 acaggaagaa gcccaaaaag tcctcaacga attcatcgca ttcacccaaa aacgcggcgt
      601 atgcggcgaa atcatccacg gcagcggatt aggttccaaa ggctacaaac ccgttctgaa
      661 aaatatgacc cgaaactggc tgatgcaaca ccccgacgta ctcgcctacg ccgaaccccg
      721 ggaaggcaac gacggctgcg tccgcatcct gctcaaacgc aaattaaggc agcaagactg
      781 acaaatttct ccgacagact tggcagcacc gcacaatatc cgtataattt gcaaaccatt
      841 tctgggggcg accttggttt cgacgggggt tgcgaagcag atgcgggcat accggggtct
      901 cagattcccg taaaacactg aattcaaata gtcgcaaacg acgaaactta cgctttagcc
      961 gcttaaggct agccgttgca gcagtcggtc aatgggctgt gtggcgaaag ccaccgcaac
     1021 gtcatcttac attgactggt ttcctaccgg gttacttggc aggaaatgag atttaaggta
     1081 actggtttcc aaaaggcctg ttggtcggca tgatggaaat aagattttca aatagacaca
     1141 actaagtatg tagaacgctt tgtagaggac tttcggacgg gggttcgatt ccccccgcct
     1201 ccaccatacc ctattttcag cagcatcctg caacacaaaa aagccttgaa cttcaacagt
     1261 ttcaaggctt ttttgttgcc ttgaatatgc tggcgtatgc ttgagttttc gccatttatg
     1321 gggtaacttt tggggtaact tttcacgaat taccccaaaa aacgccttaa aaatgcaaaa
     1381 gtcatcacgc atttttttaa aaaggttacc caaaacttaa atgtaatcct ttatttataa
     1441 gcttgcagct ttccaaacaa gttaccccca aacggataaa aaaatgccga agccgaactg
     1501 accccaaaaa gtcggacagt ttaatcaagc ggctttcagg gactgaattc tgtactgaac
     1561 aaggctcagc ccttttaatt tcaacttgat tctatcgtt
//
LOCUS       DAWHSK010000310         1595 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.310, whole genome shotgun sequence.
ACCESSION   DAWHSK010000310 DAWHSK010000000
VERSION     DAWHSK010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.310"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..548
                     /locus_tag="WIC03_002036"
     CDS             <1..548
                     /locus_tag="WIC03_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEZ3884485.1"
                     /translation="YDESGITDQPFVIVKADAGTYGMGVMSVKSADEVRGLNRKNRNK
                     MAKVKEGLEVSEVIVQEGIYTYETLNGAVCEPVVYMMDRFVIGGFFRVHEGRGADENL
                     NAGGMVFVPLSNSIPTGNGDNSQEAPEACKRVFEQWDALGMPRSEKDCDVDNEHNRLY
                     VYGVMARLSLLAASIELEETA"
     gene            671..1348
                     /gene="radC"
                     /locus_tag="WIC03_002037"
     CDS             671..1348
                     /gene="radC"
                     /locus_tag="WIC03_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEZ3884486.1"
                     /translation="MSIKQWPEGERPREKLLERGAAALSDAELLAILLRVGTRGMSAV
                     DLARYLLQEFGSLGRLMSAEVGKLSAYKGMGTASFTQFAVVREIGRRILAEELQESIV
                     LSDPDTVADYLRFHLGQEKVEVSVALLLNRQNQLIAVRELSRGTVAENTIYIREIVKL
                     ALDEYADSLIIAHNHPGGSPEPSQEDIMFTRRLAQAMSLVDVSLLDHFIVTAQTVRSF
                     RQLGLMP"
     gene            1421..>1595
                     /locus_tag="WIC03_002038"
     CDS             1421..>1595
                     /locus_tag="WIC03_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEZ3884487.1"
                     /translation="METQNKPTVTDIDRPILVPPGGHKKVLLHSCCAPCSGEVMEAML
                     ASGIDYTIYFYNPN"
BASE COUNT          390 a          392 c          465 g          348 t
ORIGIN      
        1 aatacgacga atcgggcatt accgaccagc cttttgtgat tgtcaaagcc gacgcaggta
       61 cttacggtat gggcgtgatg agcgttaaat ctgcggatga agtgcgtgga ttgaatcgta
      121 aaaaccgcaa taaaatggcg aaagtcaaag aaggcttgga agtcagcgaa gtgattgtcc
      181 aagaagggat ttatacttat gaaaccttaa acggcgcggt gtgcgaaccc gtcgtctata
      241 tgatggaccg cttcgtcatc ggcggctttt tccgcgtgca cgaagggcgc ggtgcggacg
      301 aaaatctcaa cgccggcggt atggtgtttg ttccgctgtc caacagtatt cccaccggca
      361 acggcgacaa ctcccaagaa gctcccgaag cctgcaagcg cgtattcgag caatgggatg
      421 cgctgggtat gccgcgttct gaaaaagact gcgacgtgga caacgaacac aaccgcctct
      481 acgtttacgg cgtaatggca cgcctgtcgc ttctggcggc gtccatcgag ttggaagaaa
      541 cggcgtaaga ctgttttgaa atacagatgc cgtctgaagc ggaaatccgg ttcagacggc
      601 gtttcggata tttggcgtgt gggaacattt gttccagacg gcatctcaga ctatttaaaa
      661 aagggaaaac atgagcatca agcaatggcc ggagggggaa aggcccaggg aaaagctgtt
      721 ggaacgcggg gcggcggctt tgagtgatgc cgaacttttg gcaatcctgc tgcgcgtcgg
      781 cacgcgcgga atgagtgcgg tcgatttggc gcgttatttg ctgcaggagt tcggcagttt
      841 ggggaggctg atgagcgcgg aggtcggcaa actgtcggca tacaaaggga tggggacggc
      901 aagtttcaca cagttcgccg tggtcaggga aatcgggcgg cggatattgg cggaagaatt
      961 gcaggagagc atcgtcctgt ccgatccgga taccgttgcc gattatttac gctttcattt
     1021 ggggcaggaa aaagtcgaag tcagcgtcgc gctgctgttg aaccgccaaa accaactgat
     1081 tgcggtcaga gagctgtcgc gcggtacggt tgcggaaaac acgatttaca tccgtgaaat
     1141 tgtcaaactg gcattggacg aatatgccga cagcctgatt atcgcgcaca accatccggg
     1201 cggctcgccc gaaccttcgc aggaagacat catgttcaca aggcggctgg cacaggcaat
     1261 gtcgctggtc gatgtgtcgc tgctcgacca ttttatcgta acggcgcaaa ccgtccgttc
     1321 gttcaggcag ctcgggctga tgccctgaca ccctgtttta catgcggcgg ctctgataaa
     1381 atagccgctt caaccgtatt cgacagatat cgttaagtta atggaaacac aaaacaaacc
     1441 taccgttacc gacattgacc gccccatact cgtcccgccc ggcggacata aaaaagtctt
     1501 gctgcattcc tgttgcgccc cgtgcagcgg cgaagtgatg gaggcgatgc ttgcctccgg
     1561 catcgactac accatttatt tttacaatcc caata
//
LOCUS       DAWHSK010000311         1577 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.311, whole genome shotgun sequence.
ACCESSION   DAWHSK010000311 DAWHSK010000000
VERSION     DAWHSK010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.311"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..300
                     /locus_tag="WIC03_002039"
     CDS             <1..300
                     /locus_tag="WIC03_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884488.1"
                     /translation="FGSLDGGTGMGGSIVKMAVGSQCRAELDKRSEWRLTALAMSAEK
                     QAEWENKICACVAQEAPERMTGNDVMQMLAPSTRNQALAALTAKTVSACFKHLYR"
     gene            373..>1577
                     /locus_tag="WIC03_002040"
     CDS             373..>1577
                     /locus_tag="WIC03_002040"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEZ3884489.1"
                     /translation="MKYISTRGETAHKPFSEVLLMGLAPDGGLMLPEHYPQIGREALD
                     KWRGLAYPELAFEIMRLFVTDIPEDDLRDILNRTYTEAAFGTKEITPVRTLSDGIKIQ
                     ALSNGPTLAFKDMAMQFLGNAFEYVLNKEGKKLNILGATSGDTGSAAEYALRGKKGVN
                     VFMLSPDGKMSAFQRAQMYSLQDENIHNIAVKGMFDDCQDIVKAVQNDAAFKEKYHIG
                     TVNSINWGRIVAQVVYYFAGYFNATSSNDETVSFCVPSGNFGNVCAGHIAKQMGLPVR
                     RLIVATNENDVLDEFFKTGAYRPRNSAHTYVTSSPSMDISKASNFERFVFDLMDRDPQ
                     EINTLWAEVAAGKGFDLRFALEKVGGKYGFTSGKSTHPDRLATIRQVYEQDQELIDPH
                     TADGVKVAR"
BASE COUNT          400 a          423 c          428 g          326 t
ORIGIN      
        1 ttcggcagct tagacggtgg cacaggcatg ggcggcagca tcgtcaaaat ggcggttggg
       61 agccaatgcc gtgcggaatt ggacaaacgc agcgaatggc gtttgaccgc gctggcgatg
      121 agtgccgaaa aacaggcgga gtgggaaaac aagatttgcg cttgcgtcgc ccaagaagca
      181 cccgaacgga tgaccggcaa cgatgtgatg cagatgctgg ctccgtccac gcgcaatcag
      241 gcacttgccg ccctgaccgc caaaacggtt tccgcctgct tcaaacacct gtaccgctaa
      301 ccttcttcag acggcatgag gcagatgccg tctgaagccg ttttcctgca atttcgacag
      361 acgagaaaaa tcatgaaata catcagcacg cgcggcgaaa ccgcacacaa accgttcagc
      421 gaagttttat tgatggggct tgcccccgac ggcggcctga tgctgccgga acattatccg
      481 caaatcgggc gggaagcctt ggacaaatgg cgcggtttgg cttatcccga attggcgttt
      541 gaaatcatgc gcttgttcgt tacggatatt ccggaggacg atttgcgcga tattctgaac
      601 cgtacttaca cggaagcggc gttcggtact aaggaaatca cccccgtccg cacgctttca
      661 gacggcatca aaatccaagc cttgtccaac ggcccgacgc tggcgttcaa agatatggca
      721 atgcagtttt tgggcaatgc gtttgaatat gttttaaaca aagaaggcaa aaaactcaat
      781 atcttgggcg cgaccagcgg cgatacgggt tcggctgcgg aatatgcctt gcgcggcaaa
      841 aagggcgtga acgtatttat gttgtcgccc gacggtaaaa tgagcgcgtt ccaacgcgcg
      901 caaatgtaca gcctgcaaga cgagaatatc cacaatatcg ccgtgaaggg aatgtttgac
      961 gactgtcagg acattgtgaa ggcggtgcaa aacgatgccg cgttcaagga aaaataccat
     1021 atcggtacgg tcaactcgat caactgggga cgcatcgtcg cgcaagtggt ttattacttt
     1081 gcaggctatt tcaacgccac ttcaagcaat gacgaaaccg tcagcttctg cgtaccgagc
     1141 ggcaacttcg gcaacgtttg cgcgggacat attgccaaac agatgggcct gcctgtccgc
     1201 cgcctgattg ttgcgaccaa tgaaaacgat gtgttggacg agtttttcaa aaccggcgca
     1261 taccgcccgc gcaacagcgc gcatacttat gttacctcca gcccgtctat ggacatttcc
     1321 aaagcgtcca acttcgagcg tttcgtgttc gacctgatgg atcgcgatcc tcaggaaatc
     1381 aatacgctat gggcggaagt agcggcgggc aaaggctttg acctgcggtt tgctttagaa
     1441 aaagtcggtg gcaaatacgg ttttacctcc ggcaaatcca cccaccccga ccgcctcgcc
     1501 accatcagac aggtttatga gcaagaccaa gaactcatcg acccgcacac tgccgacggc
     1561 gtaaaagtcg cccgcga
//
LOCUS       DAWHSK010000312         1570 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.312, whole genome shotgun sequence.
ACCESSION   DAWHSK010000312 DAWHSK010000000
VERSION     DAWHSK010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.312"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..409)
                     /locus_tag="WIC03_002041"
     CDS             complement(<1..409)
                     /locus_tag="WIC03_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884490.1"
                     /translation="MKNNIFLNLNKKSINNNHFVISIFFETIYQFETKDTLLECFKNI
                     TTTGHFGVIGAQYEKIDATRWIGDYEEVNGFEYIDKAPSIYFSVGDDFNPEELIIPIN
                     LAYHYFNIAISDFLIAHPEYQKKCKEIQKTYSQT"
     gene            complement(425..>1570)
                     /locus_tag="WIC03_002042"
     CDS             complement(425..>1570)
                     /locus_tag="WIC03_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VENN motif pre-toxin domain-containing protein"
                     /protein_id="HEZ3884491.1"
                     /translation="AAVGEIVGEALTNGKNPDTLTAKEREQILAYSKLVAGTVSGVVG
                     GDVNAAANAAEVAVKNNQLSDKEGREFDNEMTACAKQNNPQLCRKNTVKKYQNVADKR
                     LAASIAICTDISRSTECRTIRKQHLIDSRSLHSSWEAGLIGKDDEWYKLFSKSYTQAD
                     LALQSYHLNTAAKSWLQSGNTKPLSEWMSDQGYTLISGVNPRFIPIPRGFVKQNTPIT
                     NVKYPEGISFDTNLKRHLANADGFSQKQGIKGAHNRTNFMAELNSRGGRVKSETQTDI
                     EGITRIKYEIPTLDRTGKPDGGFKEISSIKTVYNPKKFSDDKILQMAQNAASQGYSKA
                     SKIAQNERTKSISERKNVIQFSETFDGIKFRSYFDVNTGRITNIHPE"
BASE COUNT          438 a          286 c          255 g          591 t
ORIGIN      
        1 ttgtttgaga atatgttttt tgtatttctt tacacttttt ttgatattca gggtgagcta
       61 ttaagaaatc agatattgca atattaaagt aatgatatgc taaattaata ggtataatta
      121 attcttcagg attgaaatca tctccaactg aaaaataaat agaaggagct ttatcaatat
      181 actcaaatcc atttacctct tcataatctc caatccatct ggtagcatct attttttcat
      241 attgagcacc tattactcca aaatgtccgg tagttgtaat atttttaaaa cactctaaaa
      301 gcgtatcttt agtttcaaat tggtaaattg tttcaaaaaa aatcgaaata acaaaatggt
      361 tgttatttat agatttttta tttaagttta gaaaaatatt atttttcata atttttcctt
      421 taaattattc tgggtgaatg tttgtaattc ttcctgtatt tacatcaaaa tatgatctaa
      481 atttgattcc gtcaaaggtt tctgagaatt gaatgacatt ttttctttcc gatattgatt
      541 tagttctttc attttgagca attttagagg cttttgaata tccttgtgaa gcagcatttt
      601 gagccatttg aagtatttta tcatcagaaa attttttagg attataaaca gtttttatac
      661 ttgaaatttc cttaaatcca ccatcaggtt tacctgtcct gtctagtgta ggaatctcat
      721 atttaattcg ggtaatgcct tcaatatcag tttgggtttc agattttacg cgtcctcctc
      781 gtgaatttag ttctgccata aaattggtgc ggttatgggc tcctttaatg ccctgttttt
      841 gactaaaacc atcagcattt gccagatgtc tttttaggtt tgtatcgaaa ctgatgcctt
      901 ccgggtattt gacattagta ataggtgtat tttgttttac aaaccctctt ggtattggaa
      961 tgaatctagg attaactcct gaaataagtg tataaccttg gtcggacatc cattcggata
     1021 aaggctttgt attgcccgat tgaagccaag atttagcagc agtattcaaa tgataagact
     1081 gtaaagccaa atctgcttgg gtgtaagatt tgctgaataa tttataccat tcatcatctt
     1141 taccaattag acctgcttcc caagatgaat gaaggcttct actatcgatc aaatgttgtt
     1201 ttctgattgt tctacattca gtactacggg atatatccgt acatattgca atcgaagcag
     1261 caagtctttt atcagcaaca ttttgatact tttttacagt attttttctg cacagttgag
     1321 gattattctg tttggcgcat gcagtcattt cgttatcaaa ttctctaccc tctttgtcgc
     1381 taagctgatt atttttcacc gctacctcag ccgcattcgc cgccgcattt acatcgccgc
     1441 cgaccacacc gcttaccgta ccggcaacca gtttgctgta tgccaaaatc tgttcgcgtt
     1501 ctttagctgt caaagtgtca ggatttttgc cgtttgtcaa agcctccccg actatctcgc
     1561 ccacagccgc
//
LOCUS       DAWHSK010000313         1568 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.313, whole genome shotgun sequence.
ACCESSION   DAWHSK010000313 DAWHSK010000000
VERSION     DAWHSK010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.313"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            231..1340
                     /gene="proB"
                     /locus_tag="WIC03_002043"
     CDS             231..1340
                     /gene="proB"
                     /locus_tag="WIC03_002043"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEZ3884492.1"
                     /translation="MKYKRIVFKVGTSSITHSDGSLSRGKIQTITRQLAALHHAGHEL
                     VLVSSGAVAAGFGALGFKKRPVKIADKQASAAVGQGLLMEEYTANLSSDGIVSAQILL
                     SRADFADKRRYQNAGGALSVLLQRRAVPIINENDTVSVEELKIGDNDTLSAQVAAMIQ
                     ADLLVLLTDIDGLYTGNPNSNPDAVRLDKIEHINHEIIEMAGGSGSANGTGGMLTKIK
                     AATIATESGVPVYICSSLKPDALAEAADNQADGSFFVPRAKGLRTQKQWLAFYSESRG
                     GVYVDEGAEHALSEQGKSLLMSGIAGIEGHFSRMDTVTVYSKATKQPLGKGRVLFGSA
                     AAEDLLKLRKAKGVFIHRDDWISITPEIRLLLTEF"
     gene            1353..>1568
                     /locus_tag="WIC03_002044"
     CDS             1353..>1568
                     /locus_tag="WIC03_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-phosphate reductase"
                     /protein_id="HEZ3884493.1"
                     /translation="MSNTQKQLALAKAAKKSVNTADTEEKNRALFAMADSLEAAAADI
                     LAANRQDLEAAAGKIPESMTDRLLLDGK"
BASE COUNT          429 a          412 c          420 g          307 t
ORIGIN      
        1 aaataaatca gcctacagca gaaatatgga caggattata cgcattttgg acaaaacggc
       61 aatataaatt tgaaaaaatc tcaataaacc gccatagatt ttattaattt tatacatttt
      121 gtccatttta ttgcagacgc ggcgtgattg ttgacaattg cggcagaaca gggcaaattc
      181 ggcggcacaa ggtggcaacg agacaggaca aaccaacaag gacggaaaca atgaaataca
      241 aaagaatcgt atttaaagtc ggcacatctt cgattaccca ttcggacggc agtctctcgc
      301 gcggcaaaat ccaaaccatc acccgccagc ttgccgcatt gcatcatgcg ggacacgagc
      361 tggtcttggt gtcttccggc gcggttgcgg cagggttcgg tgcgctgggt ttcaaaaaac
      421 gtccggtcaa aatcgccgac aaacaggctt ccgccgccgt cgggcagggg ctgctgatgg
      481 aagaatatac ggcaaacctg tcttcagacg gcatcgtgtc cgcgcaaatc ctgctcagcc
      541 gcgccgactt tgccgacaaa cgccgctacc aaaatgccgg cggcgcactt tccgtgctgc
      601 tgcaacgccg cgccgtcccc atcatcaatg aaaacgatac ggtttcggtt gaggaattga
      661 aaatcggcga caacgacaca ttgagtgcgc aagtggcggc gatgatacag gcagacctct
      721 tggtgctgct gaccgacata gacggtcttt acacgggcaa cccgaacagc aatcccgatg
      781 ccgtacggct ggacaaaatc gaacacatca accatgaaat catcgaaatg gcgggcggat
      841 cgggttcggc aaacggcacg ggcggtatgc tgactaaaat caaagcggcg acgattgcga
      901 ccgagtccgg cgtaccggtc tatatctgtt cctcgctcaa acccgatgca cttgccgaag
      961 cggcagataa tcaggcggac ggctcgtttt tcgtcccccg tgccaaaggt ttgcggacgc
     1021 agaagcaatg gctggcgttc tattccgaaa gcaggggcgg cgtttatgtg gacgaaggtg
     1081 cggaacacgc tttgtccgaa cagggaaaaa gcctgctgat gtcgggcatt gccggaatcg
     1141 aagggcattt ttcccgtatg gacaccgtaa ccgtgtacag caaggcaacc aaacagcctt
     1201 tgggcaaagg gcgagtcctg ttcggctctg ccgccgccga agacctgctc aaattgcgta
     1261 aggcgaaagg cgtgttcatc catcgggacg actggatttc catcacgccc gaaatacgcc
     1321 tgcttctgac cgaattttag aaaagaaaac ccatgtcaaa cacacaaaaa cagcttgccc
     1381 ttgccaaagc ggcaaaaaaa tccgtcaaca cggcggatac ggaagaaaaa aaccgcgcgc
     1441 tgtttgctat ggcggacagc ttggaagcgg cggcggcaga tattttggcg gcaaaccgtc
     1501 aagacttgga agccgcggca ggcaaaattc ccgaaagcat gactgaccgc cttttgttgg
     1561 acggcaaa
//
LOCUS       DAWHSK010000314         1565 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.314, whole genome shotgun sequence.
ACCESSION   DAWHSK010000314 DAWHSK010000000
VERSION     DAWHSK010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.314"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(207..1196)
                     /gene="porB"
                     /locus_tag="WIC03_002045"
     CDS             complement(207..1196)
                     /gene="porB"
                     /locus_tag="WIC03_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric porin PorB"
                     /protein_id="HEZ3884494.1"
                     /translation="MKKSLIALTLAALPVAAMADVTLYGTIKAGVETSRSVAHNGAQA
                     ASVETGTGIVDLGSKIGFKGQEDLGNGLKAIWQVEQKASIAGTDSGWGNRQSFIGLKG
                     GFGKLRVGRLNSVLKDTGDINPWDSKSDYLGVNKIAEPEARLISVRYDSPEFAGLSGS
                     VQYALNDNAGRHNSESYHAGFNYKNGGFFMQYGGAYKRHQNVDNVKIEKYQIHRLVSG
                     YDNDALYASVAVQQQDAKLVKDNSHNSQTEVAATLAYRFGNVTPRVSYAHGFKGSFDD
                     ADYTNDYDQVVVGAEYDFSKRTSALVSAGWLQEGKGESKFVSTAGGVGLRHKF"
BASE COUNT          409 a          352 c          397 g          407 t
ORIGIN      
        1 tcccgcctgc gcgggaatga cggcatattt tgacattgaa taaaaaagac taaaaacagg
       61 aaaaagccaa acagaaaaca gcgaaacaga aaaaagccaa acagaaaaaa gccaaacaga
      121 aaacagaaaa cagccaaaca ggaaaaagcc aaacagaaaa aagcctgtct ggcgacaggc
      181 tttttgttga taccaatctt tgcagattag aatttgtggc gcagaccgac accgccggca
      241 gtcgatacga atttgctttc gcctttgcct tcttgcaacc aaccggcaga aaccaaggca
      301 gaagtgcgtt tggagaagtc gtattccgca ccgacaacca cttggtcgta atcgttggtg
      361 tagtctgcat catcaaacga gcctttgaag ccgtgggcgt aagaaacgcg gggcgttacg
      421 ttgccgaagc ggtatgccaa ggtagcggca acttcggttt gagagttgtg cgaattgtct
      481 ttaaccagtt tcgcgtcttg ttgctgtacg gctacggaag cgtacagggc atcattgtcg
      541 taaccgctga ccaaacggtg aatctggtat ttctcaatct tcacgttatc cacattctga
      601 tgtcttttat aggcaccgcc atattgcatg aagaagccgc cgtttttgta gttgaagccg
      661 gcgtggtaag attcgctgtt atgtctgcct gcattgtcgt taagtgcgta ttgtacgctg
      721 ccgctgaggc cggcaaattc gggagaatcg tagcgtacgg aaatgaggcg tgcctcgggt
      781 tcggcaattt tgtttacacc caaatagtcg cttttgctat cccaaggatt gatgtcgccg
      841 gtatctttca ggacgctgtt caaacggccg acgcgcaatt taccgaagcc gcctttcaaa
      901 ccgatgaagg attggcggtt gccccaaccg gagtcagtac cggcgataga tgccttttgc
      961 tcaacctgcc aaatggcttt cagaccgtta ccgaggtctt cttggccttt gaagccgatt
     1021 ttcgaaccca aatcaacgat gccggtaccg gtttcaacgc tagccgcctg agctccattg
     1081 tgagctacgg agcgggaagt ttctacgccg gctttgatgg tgccgtacag ggtaacgtca
     1141 gccattgctg caacaggaag ggctgccaaa gtcagggcaa tcagggattt tttcattgct
     1201 gtattccttt tttggttaag aaatttaagc aggccgggct ttccaagccg cttagctttg
     1261 catttaccgc cgacgttttc ggcgatttca tgtctgaaac tataaatgtt cctgtctgaa
     1321 aaaacaaact tctgcctgtt tttgtgtgga atccgatgtg cattttgaag gatggataga
     1381 gatattatgg gtatttttgt tttataacat atggttattt aaatttttta agatttgcat
     1441 ttttacaaca cttactcggg agggtattgg agggcattgc aaaccggggg ttataaagac
     1501 ggcaaaaaac gcgcacgtgg tttctttcgg aacgggcgga tgcaaaaccc gaccctgcgg
     1561 cagga
//
LOCUS       DAWHSK010000315         1531 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.315, whole genome shotgun sequence.
ACCESSION   DAWHSK010000315 DAWHSK010000000
VERSION     DAWHSK010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.315"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            53..1219
                     /locus_tag="WIC03_002046"
     CDS             53..1219
                     /locus_tag="WIC03_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEZ3884495.1"
                     /translation="MRKKLTALVLSALPLAAVADVSLYGEIKAGVEGRNYQLQLTEQP
                     SRTQGQTSNQVKVTKAKSRIRTKISDFGSFIGFKGSEDLGEGLKAVWQLEQDVSVAGG
                     GATRWGNRESFVGLAGEFGTLRAGRVANQFDDASKAIDPWDSNNVVASQLGIFKRHDD
                     MPVSVRYDSPDFSGFSGSVQFVPAQNSKSAYKPAYVDEKKMVHAAVVGKPGSDVYYAG
                     LNYKNGGFAGNYAFKYAKHANVGRDAFNLFLLGRIGDDDEAKGTDPLKNHQVHRLTGG
                     YEEGGLNLALAAQLDLSENGDKTKNSTTEIAATASYRFGNAVPRISYAHGFDFIERGK
                     KGEHTSYDQIIAGVDYDFSKRTSAIVSGAWLKRNTGIGNYTQINAASVGLRHKF"
BASE COUNT          392 a          366 c          396 g          377 t
ORIGIN      
        1 gtgtttgccc gatgttttta ggtttttatc aaatttacaa aaggaagccg atatgcgaaa
       61 aaaacttacc gccctcgtat tgtccgcact gccgcttgcg gccgttgccg atgtcagcct
      121 gtacggcgaa atcaaagccg gcgtggaagg caggaactac cagctgcaat tgactgaaca
      181 accctcaaga actcaaggtc aaacgagcaa tcaggtaaaa gttactaagg ccaaaagccg
      241 catcaggacg aaaatcagtg atttcggctc gtttatcggc tttaagggga gcgaggattt
      301 gggcgaaggt ctgaaggctg tttggcagct tgagcaagac gtatccgttg ccggcggcgg
      361 cgcgacccgt tggggcaaca gggaatcctt tgtcggcttg gcaggcgaat tcggcacgct
      421 gcgcgccggc cgcgttgcga atcagtttga cgatgccagc aaagccattg atccttggga
      481 cagcaataat gttgtggctt cgcaattggg tattttcaaa cgccacgacg atatgccggt
      541 ttccgtacgc tacgactctc cggacttttc cggtttcagc ggcagcgtcc aattcgttcc
      601 ggctcaaaac agcaagtccg cctataagcc ggcttatgtg gatgagaaga aaatggttca
      661 tgcggctgtt gtcggcaagc ccggatcgga tgtgtattat gccggtctga attacaaaaa
      721 tggcggtttt gccgggaact atgcctttaa atatgcgaaa cacgccaatg tgggccgtga
      781 tgcttttaat ttgttcttgc ttgggcgcat cggcgatgat gatgaagcca aaggtaccga
      841 tcccttgaaa aaccatcagg tacaccgcct gacgggcggc tatgaggaag gcggcttgaa
      901 tctcgccttg gcggctcagt tggatttgtc tgaaaatggc gacaaaacca aaaacagtac
      961 gaccgaaatt gccgccactg cttcctaccg cttcgggaat gcagttccac gcatcagcta
     1021 tgcccatggt ttcgacttta tcgaacgcgg taaaaaaggc gaacatacca gctacgatca
     1081 aatcatcgcc ggcgttgatt atgatttttc caaacgcact tccgccatcg tgtctggtgc
     1141 ttggctgaaa cgcaataccg gcatcggcaa ctacactcaa attaatgccg cctccgtcgg
     1201 tttgcgccac aaattctaaa tatcggggcg gtgaagcgga tagctttgtt tttgacggct
     1261 tgccttcatt ctttgattgc aatctgactg ccaatctgct tcagccccaa acaaaaatcc
     1321 ggatacggaa gaaaaacggc aataaagaca gcaaataccg tctgaaagat tttcagacgg
     1381 tatttcgcat tttcggcttg gtttgcacat atagtggggg actgttagtt tttgcacaag
     1441 gaacaaatag agtaaaaaaa cgctgaaatc tcggaaagac gtggatttcg gcgttttttt
     1501 gcatccggaa aagttacgcc agctttttca c
//
LOCUS       DAWHSK010000316         1510 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.316, whole genome shotgun sequence.
ACCESSION   DAWHSK010000316 DAWHSK010000000
VERSION     DAWHSK010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.316"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            198..410
                     /locus_tag="WIC03_002047"
     CDS             198..410
                     /locus_tag="WIC03_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884496.1"
                     /translation="MPHIHPLHLFPPHIAERGLLYFQQGKVGAVQKTSTGRYRAEVCG
                     LENYWVWLKLDSDLYIKDESCDCPYV"
     gene            707..862
                     /locus_tag="WIC03_002048"
     CDS             707..862
                     /locus_tag="WIC03_002048"
                     /inference="COORDINATES: protein motif:HMM:NF017953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HEZ3884497.1"
                     /translation="MSERKTKPSPKKGVVFPFIKKLLSINEINPNDPTIGFILLSRNN
                     PDTDYES"
     gene            complement(952..1401)
                     /locus_tag="WIC03_002049"
     CDS             complement(952..1401)
                     /locus_tag="WIC03_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HEZ3884498.1"
                     /translation="MTDNRGFTLVELISVVLILSVLALIVYPSYRNYVEKAKINAVRA
                     ALLENAHFMEKFYLQNGRFKQTSTKWPSLPIKEAEGFCIRLNGIARGALDSKFMLKAV
                     AIDKDKNPFIIKMNENLVTFICKKSASSCSDGLDYFKGNDKDCKLLK"
BASE COUNT          478 a          361 c          234 g          437 t
ORIGIN      
        1 gggattttgg ggagttttgc aaaggtctca gtctttttaa accacagccc ttcccaacta
       61 aaccaaaagg tcgtctgaac cctattttca gacgaccttt tgccactttg taaaacaaat
      121 cttcccacca tcctctcccc aaacatcgcc cgaaccagta aacttctcat atttcaacaa
      181 cttcttggaa gcaaaccatg ccccatatcc acccgctcca cctgttcccg ccccatatcg
      241 ccgaacgcgg actgctctat tttcagcagg gcaaagttgg tgctgtccaa aaaacttcca
      301 ccgggcgtta tcgggcggag gtatgcggtt tggaaaacta ttgggtatgg ctgaagctgg
      361 atagtgattt gtatattaaa gacgaaagct gcgattgccc ttatgtctaa aggtgcaaac
      421 ataccttaaa ttactatatt acataggcaa aatacaagcc tataacgaat tggaaacaaa
      481 atgccgtctg aaagcattat aaaatctgtt caccttttca gatgagtaat gtacacccct
      541 atacaatttt tgctactata ccccataaat ccacggctaa agatatcctt attatgtcct
      601 atgatttatc gaaacgactt gtaatcggct tagcatcaag tgccctattc gacttatccg
      661 aatcggataa tatatttaga atggaagggg cagaaaccta taggcaatat cagagagaaa
      721 aacaaaacca tcccctaaaa aaggcgttgt ctttccattt attaaaaaac ttctgtcaat
      781 caatgaaata aacccaaacg acccaacaat tgggtttatt cttttatcca gaaacaatcc
      841 agatacagat tacgagtcat aactataggc ttaatattac acgattctca ttccatcaag
      901 gcggaaaacc gcacaaatac tgaaacacta tcgatcgatt tgtaaacaag cctacttaag
      961 taacttgcag tccttatcat ttcctttaaa ataatccagc ccgtcactac acgaactggc
     1021 ggacttcttg caaataaagg ttactagatt ttcattcatc ttaataataa aaggattttt
     1081 atctttatct atggctaccg ccttcaacat gaatttactg tctaaggccc cgcgcgcgat
     1141 tccattcaaa cggatacaaa agccttctgc ctctttaatc ggcaaacttg gccatttggt
     1201 agatgtttgt ttaaatctcc cattctgcag ataaaacttt tccataaaat gtgcattttc
     1261 taacaaggct gcccgcactg catttatctt tgctttctca acataattgc gatagctcgg
     1321 ataaacaatt aaagcaagta cagacaatat caagaccact gatattaatt caaccagcgt
     1381 aaacccccga ttatcagtca ttactttact tccaataaga acagattatt caacatattt
     1441 ctttgaacag acttactatc ccattcagca gtatgcatat ttcccactct attttttatc
     1501 cgcgggcagc
//
LOCUS       DAWHSK010000317         1498 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.317, whole genome shotgun sequence.
ACCESSION   DAWHSK010000317 DAWHSK010000000
VERSION     DAWHSK010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1498
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.317"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            31..1425
                     /locus_tag="WIC03_002050"
     CDS             31..1425
                     /locus_tag="WIC03_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEZ3884499.1"
                     /translation="MTPSALKKTVLLLGTAFAAASAQASGYHFGTQSVNAQSTANAAA
                     AEAADASTIFYNPAGLTKLDSSQISVNANIVFPSIRYEADSATDFTGIPVQGSKSGKI
                     TKTTVAPHIYGAYKVNDNLTVGLGVYVPFGSATEYEKDSVLRHNINKLGLTSIAVEPV
                     AAWKLNERHSFGAGIIAQHTSAELRKYADWGIPKKAQMLQATPSNPTAAAQIKADGHA
                     DVKGSDWGFGYQLAWMWDINDRARVGVNYRSKVSHTLKGDAEWAADDAMAKQLWNSNM
                     LAPLGYTANEKARVKIVTPESLSVHGMYKVSDKADLFGDVTWTRHSRFDKAELVFEKE
                     KTIVNGKSDRTTITPNWRNTYKVGFGGSYQISEPLQLRAGIAFDKSPVRNADYRMNSL
                     PDGNRIWFSAGMKYHIGKNHVVDAAYTHIHINDTSYRTAKASGNDVDSKGASSARFKN
                     HADIIGLQYTYKFK"
BASE COUNT          397 a          495 c          342 g          264 t
ORIGIN      
        1 tccaccgtcc gcctttttac ggaaaccgaa atgacccctt ccgcactgaa aaaaaccgtc
       61 ctgctgctcg gcactgcctt tgccgccgca tccgcacaag cctccggcta ccacttcggc
      121 acacagtcgg tcaacgcgca aagcacggcg aatgccgccg ccgcagaagc cgccgacgca
      181 tcgaccatct tctacaaccc cgccggcctg accaaactcg acagcagcca gatttccgtc
      241 aacgccaata tcgtatttcc aagcatccgt tatgaagcgg attccgccac cgactttacc
      301 ggcattcccg tccaaggttc gaaaagcggc aaaatcacca aaaccacggt tgcgccccac
      361 atctacggcg catacaaagt caacgacaat ctgaccgtag gcttgggcgt gtacgtcccc
      421 ttcggctctg ccaccgaata cgaaaaagat tccgtattgc gccacaacat caacaaactc
      481 ggtctgacca gcatcgccgt cgaacccgtc gccgcgtgga aactcaacga acgccattcc
      541 ttcggcgcag gcatcatcgc ccaacatact tctgccgagc tgcgcaaata tgccgactgg
      601 gggatcccaa aaaaagcgca aatgctgcaa gcaacacctt ctaatcctac tgccgctgct
      661 caaatcaaag ccgacggaca cgccgatgtc aaaggcagcg attggggctt cggctaccaa
      721 ctggcgtgga tgtgggacat caacgaccgc gcacgcgtgg gcgtgaacta ccgttccaaa
      781 gtctcacaca cgctcaaagg cgatgccgaa tgggcggcag acgacgcaat ggcgaaacaa
      841 ctgtggaata gcaatatgct cgcaccgctc ggctacacgg cgaatgaaaa agcccgcgtt
      901 aaaatcgtta cgcctgagtc tttgtccgta cacggtatgt acaaagtgtc cgataaagcc
      961 gacctgttcg gcgacgtaac ttggacgcgc cacagccgct tcgataaggc ggaactggtt
     1021 tttgaaaaag aaaaaaccat cgtcaacggc aaatccgacc gcaccaccat cacccccaac
     1081 tggcgcaaca cctacaaagt cggcttcggc ggttcttatc aaatcagcga accgctgcaa
     1141 ctgcgcgccg gcatcgcttt tgacaaatcg cccgtccgca acgccgacta ccgcatgaac
     1201 agcctgcccg acggcaaccg catctggttc tccgccggca tgaaatacca tatcggcaaa
     1261 aaccacgtcg tcgatgccgc ctacacccac atccacatca acgacaccag ctaccgcacg
     1321 gcgaaggcaa gcggcaacga tgtggacagc aaaggcgcgt cttccgcacg tttcaaaaac
     1381 cacgccgaca tcatcggcct gcaatacacc tacaaattca aataaacggc ccgccgtttg
     1441 attgtagaaa tgccgtctga aacagatttc cgtttcagac ggcattgttt taggctcg
//
LOCUS       DAWHSK010000318         1498 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.318, whole genome shotgun sequence.
ACCESSION   DAWHSK010000318 DAWHSK010000000
VERSION     DAWHSK010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1498
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.318"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            175..1233
                     /gene="alr"
                     /locus_tag="WIC03_002051"
     CDS             175..1233
                     /gene="alr"
                     /locus_tag="WIC03_002051"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEZ3884500.1"
                     /translation="MRPLNVQIRLGNLRHNYRILKEMHGGKLLAVVKADAYGHGAVRC
                     AFALADLADGFAVATIDEGIRLRESGITHPIVLLEGVFEASEYEAVEQYSLWPAVGNQ
                     WQLEALLSRHWKKPVKVWLKMDSGMHRTGFFPHDYASAYAALKQSEYVDSIVKFSHFS
                     CADEPESGITEIQMEAFDLGTEGLEGEESLANSAAILNVPEARRDWGRAGLALYGISP
                     FGGSDDRLKPVMRLSTRIFGERVLQPHSPIGYGATFYTSKSTRVGLIACGYADGYPRR
                     APSNSPVAVDGKLTRVIGRVSMDMMTIELDASQEGLGHEVELWGDTVNINTVAEAAGT
                     IPYELMCNIKRAKFTYIE"
BASE COUNT          386 a          356 c          385 g          371 t
ORIGIN      
        1 tttgggaaag cgattatatc aggaaaagca aaccgccttc ctacctgaaa actgctgctt
       61 cggcttgaag acacaagggt ctttaatatt ttaaaagcct tgccgttgga ttataattcc
      121 ctaattgatt tcttaatttt gctaataaac acttgcttgg taaggaatga atttatgcgc
      181 cctttgaacg tgcagatcag gttgggcaac cttaggcaca attatcggat tttaaaggaa
      241 atgcacggag gcaagctgtt ggcggtagtg aaggccgacg catacggaca cggtgcggtc
      301 agatgtgctt tcgcgctggc agacttggca gacggctttg ccgtggcgac aatcgatgaa
      361 ggaatcaggc tgcgggagag cggcattacc catccgattg tccttttgga aggcgtattt
      421 gaagcatcag agtacgaagc ggtcgaacaa tactcgctct ggccggcagt cggaaaccaa
      481 tggcaacttg aggctttgct ttcccgccat tggaaaaaac ctgtcaaggt ctggttgaaa
      541 atggattcgg gaatgcaccg taccggtttt ttccctcatg attacgcttc ggcatatgcg
      601 gcattgaagc agtcggaata tgtggacagt attgtcaaat tctcgcattt ctcctgtgcg
      661 gacgaacccg aaagcggtat aacggaaata cagatggaag cattcgattt gggtactgaa
      721 gggctggaag gcgaagaaag ccttgccaac tccgccgcta ttttgaatgt tcccgaagca
      781 cgcagggact gggggcgcgc gggcttggcg ttgtacggca tttccccgtt cggaggcagc
      841 gatgacaggc tgaaacccgt gatgaggctt tccacccgta ttttcggcga acgcgtttta
      901 cagccgcact cccctatcgg ttatggcgca acattttata ccagcaaatc tacgcgggtc
      961 ggcctgattg cctgcggtta tgcggacggt tatccgcgcc gtgccccaag caattccccc
     1021 gtcgccgtcg acggtaaatt gacccgggtc atcggcaggg tctctatgga tatgatgacc
     1081 atcgagctgg acgcttcaca agaaggtttg ggacacgagg tcgaactgtg gggcgatacg
     1141 gtcaacatca ataccgttgc cgaagcggcc ggaaccatcc cttacgaatt gatgtgcaat
     1201 atcaaacgtg caaaattcac ttatatcgag taaccgggtt caaacgaaaa tgccgtctga
     1261 agcctttcag acggcataaa tgcgttttga caaaatcagt aaaagacatt gccaaataga
     1321 aaatactgat aatgcccaca atctgaaacc ccatccttcc cgcaaaaacg gaaaatcaaa
     1381 atcagaaacc taaaatttcg tcattcccgc gcaggcggga atccggtttt ttgagtttcc
     1441 gtcatttctg ataaattctc gtcgtttttc atttctagat tcccgcctgc gcgggaat
//
LOCUS       DAWHSK010000319         1496 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.319, whole genome shotgun sequence.
ACCESSION   DAWHSK010000319 DAWHSK010000000
VERSION     DAWHSK010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1496
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.319"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..287)
                     /locus_tag="WIC03_002052"
     CDS             complement(<1..287)
                     /locus_tag="WIC03_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEZ3884501.1"
                     /translation="MTTLKPALPAYLDNIRIILTRTSHPANIGSAARAMKTMGLHKLT
                     IVAPNLMATPMTENPPVFDPEHPQSFKLPEESFILASGAADVLENATIAAS"
     gene            351..521
                     /locus_tag="WIC03_002053"
     CDS             351..521
                     /locus_tag="WIC03_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884502.1"
                     /translation="MPSEMFFNNVGNLTLRLKQTRVGRYFTRFGRIYPNQINSDFAAP
                     KDCASEPAVCHP"
     gene            531..1316
                     /locus_tag="WIC03_002054"
     CDS             531..1316
                     /locus_tag="WIC03_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HEZ3884503.1"
                     /translation="MNPFLNTAFKAARRAGQMMIRAAGNLDAVKTDSKAFNDFVSDVD
                     RNSEIILVEALKEAYPHHKITCEESGSHGKAAAEYEWIIDPLDGTTNFLHGHPQYAIS
                     MALLHKGVLQEALVYAPERNDVYMASRGKGALLNDRRIRVSNRIELNRCLIGTGFPVV
                     DQSMMDKYLAILKDFLAKTAGGRREGAASLDLCAVATGRFDGFFEFNLKPWDIAAGAL
                     IVQEAGGIVTDMTGEDGWLESGDIVAANPKVLAQMLKIISAHV"
BASE COUNT          349 a          375 c          420 g          352 t
ORIGIN      
        1 aaagaagcgg caatggtggc attttccaaa acgtctgccg cgccggaagc gaggatgaag
       61 ctttcttccg gtaatttaaa cgattgagga tgctccgggt caaacacggg cgggttttcc
      121 gtcatcggcg ttgccatcag atttggggcg acgatggtca gtttgtgcag acccattgtt
      181 ttcatcgcgc gcgcggcaga gccgatgttg gcgggatggc tggtgcgcgt gaggatgatg
      241 cggatgttgt ccagataagc gggcagggcg ggtttgagag tagtcatgtt cggttttcgg
      301 tcggaatgga atagggtata atgcgccgtc tttttcgttc ggacggtcaa atgccgtctg
      361 aaatgttctt taacaacgtc ggaaatttaa cgttgcgcct gaagcagaca cgcgtcgggc
      421 ggtattttac ccgatttggc aggatttacc ccaaccaaat aaactccgat tttgccgctc
      481 cgaaggactg cgcgtccgaa ccggcggtat gccaccctta aggaaatctg atgaatccgt
      541 ttttgaatac agcctttaaa gccgcccgcc gtgccggtca gatgatgata cgcgccgcgg
      601 gcaatctcga tgccgtcaaa accgacagca aagcattcaa cgattttgtt tccgatgttg
      661 accgcaattc cgaaatcatc ctggttgagg ctttgaaaga agcctatccg caccacaaaa
      721 tcacttgcga agaaagcggc tcccacggta aagccgcagc cgaatacgaa tggattatcg
      781 atccgctcga cggcacgacc aacttccttc acggccatcc ccaatacgcc atctctatgg
      841 cactcctgca caaaggcgtg ttgcaagaag ctttggtgta cgcccccgaa cgcaacgacg
      901 tttacatggc ttcgcgcggc aaaggcgcgt tgctcaatga ccgccgcatc cgcgtttcca
      961 accgcatcga attgaaccgc tgcctgatcg gtaccggctt tcctgttgtc gatcaaagca
     1021 tgatggacaa gtatctggcg attttgaaag atttcttggc aaaaaccgcc ggcggccgtc
     1081 gtgaaggcgc ggcttctttg gatttgtgtg ctgtggcgac tggtcgtttt gacggctttt
     1141 tcgagttcaa cctcaaacct tgggatattg ccgcaggcgc attgattgtt caggaagcgg
     1201 gcggtatcgt aaccgatatg acgggtgaag acggctggct ggaaagcggc gatattgttg
     1261 ccgccaatcc caaagtgctg gcgcaaatgt taaaaatcat ttccgcacac gtttaaattt
     1321 cagcccgctc ccgggaatgc gggaaaacgg gcgggtatgc cgtctgaagt atcgcgccga
     1381 tatttcagac ggcattttta ctggcagcgg gggcaggaag gtggaaacgg ctaaatgccc
     1441 gaaactgccg aagatggaaa cccatatctg attaaacgga gaaccctgta cgattt
//
LOCUS       DAWHSK010000320         1482 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.320, whole genome shotgun sequence.
ACCESSION   DAWHSK010000320 DAWHSK010000000
VERSION     DAWHSK010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.320"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..1389
                     /locus_tag="WIC03_002055"
     CDS             <1..1389
                     /locus_tag="WIC03_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEZ3884504.1"
                     /translation="QQHTRQYSQAQQRAAELLARTNQGQIRREHIERELAQLAEEQTV
                     LQHASDGLSDDIATLQEAAAELEHQQQTAAHSRQEQQGRLKQAQLALLEANRQYGLAE
                     VAVHKLNQQKQNYRQQIARLEQQTLDWQERQQELALAYETEFQNDEQHIKLEELSEAV
                     QSLDEEYIVVQEKLAQIQEQGREQYARVQTLQTKLPQLQAATQTALLQQQEALINAKR
                     YHQNLTERAADLDALEALAKESPKVLNSSIGSLTRQIEALGAVNLAALQELEEARERD
                     GYYRSQSEDVQAAITLLEEAIAQIDDKTKARFKETFDAVNGKVQTFFPTLFGGGEATL
                     KMIGDDLLTAGVSIMARPPGKKNSTIHLLSGGEKALTAMSLVFALFSLNPAPFCLLDE
                     VDAPLDDANTSRFCNLVKEMSAQTQFLYISHNRLTMEMAEQLVGVTMQEKGVSRVVAV
                     DIKQALEMAEAV"
BASE COUNT          403 a          467 c          380 g          232 t
ORIGIN      
        1 cagcagcaca cgcgccaata cagccaagcg cagcaacgcg ccgccgaact tttggcgcgt
       61 accaaccaag ggcaaatccg ccgcgaacac atcgagcgcg aactggcgca gttggcggaa
      121 gagcagaccg tgttgcaaca cgcgtccgac gggctttcag acgacattgc tactttgcag
      181 gaagccgccg ccgaactcga acaccagcag caaaccgccg cacacagccg ccaagagcag
      241 caaggccgtc tgaaacaggc gcagcttgcc ctgctggaag ccaaccgcca atacgggctg
      301 gccgaagtcg ccgtccacaa gctcaaccag caaaaacaaa actaccggca gcaaatcgcc
      361 cgactcgaac agcaaaccct agactggcag gaacgccagc aagagcttgc ccttgcctat
      421 gaaaccgagt ttcaaaacga tgagcagcac atcaagctcg aagagttgag cgaagccgta
      481 cagagtttgg acgaagaata tattgtcgta caggagaaac tcgcgcagat tcaggagcag
      541 ggtagggaac aatacgcccg cgtacaaacc ctgcaaacca agctgccgca gcttcaggcc
      601 gccacccaaa ccgccctgtt gcaacagcag gaagccctga tcaacgccaa acgctaccat
      661 caaaacctga ccgaacgcgc cgccgatttg gacgcgctcg aagcgttggc gaaagaatcg
      721 ccgaaagtat tgaacagcag catcggcagc cttacccggc aaatcgaagc cctcggcgcc
      781 gtcaacctcg ccgccctgca agaactcgaa gaagcgcgcg aacgcgacgg ctactaccgc
      841 agccagagcg aagacgtgca ggcagccatt acccttttgg aagaagccat cgcccaaatc
      901 gacgacaaaa ccaaagcgcg tttcaaagaa accttcgatg ccgtcaacgg caaagtccaa
      961 accttcttcc cgaccctgtt cggcggcggc gaagccaccc tcaaaatgat aggcgacgac
     1021 ctcctgaccg caggcgtatc catcatggcg cgtccgcccg gcaagaaaaa cagcaccatc
     1081 cacctcctct ccggcggcga aaaagccctt accgcgatga gcctcgtgtt cgccctgttc
     1141 agcctcaacc ccgctccgtt ctgtcttttg gacgaagtcg acgctccgct agacgatgcc
     1201 aacacttcgc gtttctgcaa cctggtcaaa gaaatgtcgg cgcaaaccca gttcctctac
     1261 atctcccaca accgcctgac gatggaaatg gcggagcagc tcgtcggcgt aacgatgcag
     1321 gaaaaaggcg tatcgcgcgt cgtcgccgtg gacatcaaac aggcgttgga aatggcggaa
     1381 gccgtttgaa cgggttgcag aacggctgaa tcttgccgtt tttaatgacg tgttgcgata
     1441 cgggttttca gacggtatag tgaattaaat ttaaatcggt at
//
LOCUS       DAWHSK010000321         1441 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.321, whole genome shotgun sequence.
ACCESSION   DAWHSK010000321 DAWHSK010000000
VERSION     DAWHSK010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1441
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.321"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(80..1417)
                     /locus_tag="WIC03_002056"
     CDS             complement(80..1417)
                     /locus_tag="WIC03_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEZ3884505.1"
                     /translation="MSNHTSWSSKIGFVLAAAGSAIGLGAIWKFPYTAGTNGGAVFFL
                     LFLIFTILVALPVQLAEFYIGRTGGKNAVDSFRALRPGTQWLWVGRMGVAACFILLSF
                     YSVVGGWVLNYVVHSFTGAIHAGADFEALFGTTISNPAGSLSYQALFMLITVWVVKGG
                     ISDGIEKANRYLMPGLFILFIALAIRSLTLPGAMEGVSFLLKPNWSYFKADTMITALG
                     QAFFALSIGVSAMITYASYLGKDQDMFRSGHTIMWMNLLVSLLAGLVIFPAVFAFGFE
                     PSQGPGLIFIVLPAVFMKMPFGTVLFAVFMLLVVFATLTSAFSMLETVIASTIRQDER
                     KRKKHTWLIGTAIFIVGIPSALSFGVWGEFKVFGKTIFDLWDYVISAVIMPIGALSVA
                     VFTAWIQDKQSVLKDAGAGSTVPRAVLLLWLNTLRYLAPIAIIIVFVNSLDIL"
BASE COUNT          444 a          383 c          351 g          263 t
ORIGIN      
        1 tgaacgaaat aggctgaacc tgaagcaaaa atgccgtctg aaaggctttc agacggcatt
       61 tttgctgttt ggatggcttt taaaggatgt ccaaagaatt gacgaaaaca ataataatgg
      121 caatcggggc gaggtagcgc aaggtattca gccacagcag cagcactgcc cgtggtacgg
      181 tgctgcccgc gccggcatct tttaacacag actgcttgtc ctgaatccag gcggtaaata
      241 cggcaacgct caaagcacca atcggcataa tgacggcgga aataacatag tcccacaaat
      301 caaaaatggt tttgccgaaa accttaaact caccccatac gccgaaagac agcgcggacg
      361 ggatgccgac aatgaaaata gccgtgccga taagccaagt gtgctttttg cgtttgcgct
      421 cgtcttggcg gatggttgag gcaatgaccg tttccaacat cgaaaatgcg gaagtcagcg
      481 tggcgaaaac gaccagaagc atgaataccg caaacaaaac cgtaccgaac ggcatcttca
      541 taaacactgc gggcaatacg ataaaaatca atcccggccc ctggctcggt tcaaaaccga
      601 aggcgaacac cgccggaaaa atcaccaggc cggcaagcag cgaaaccaag aggttcatcc
      661 acataatcgt atggccggaa cggaacatat cctgatcttt tcccaaatat gaagcgtagg
      721 taatcatggc ggaaacgccg atgctcaggg caaaaaacgc ctgtcccaaa gccgtaatca
      781 tcgtatcggc tttaaagtac gaccaattcg gtttgagcag gaaagacacg ccctccattg
      841 cacccggcag cgtcagcgaa cggattgcca gcgcaataaa gaggataaac agccccggca
      901 tcagataacg gtttgccttt tcaatgccgt ctgaaatccc gcctttgacc acccaaaccg
      961 taatcagcat aaacagtgcc tgataggaca gcgaacctgc cggattggaa atcgttgtgc
     1021 cgaacagggc ttcaaagtcc gctcctgcat ggattgcccc tgtaaaactg tggacgacat
     1081 aattcaatac ccatccgccg accacgctgt aaaacgacag caaaataaag caggcggcaa
     1141 cgcccatgcg cccgacccaa agccattgcg tgcccggacg cagagccctg aaggaatcga
     1201 cggcattttt accgcccgtg cgcccgatat aaaactcggc aagctggacg ggcagggcga
     1261 ccaagatagt aaatatcaaa aacagcagga aaaacaccgc gccgccgttg gtgcctgccg
     1321 tataagggaa tttccaaatc gcgcccaaac cgatggccga acctgccgca gcaaggacga
     1381 aaccgatttt ggacgaccaa gaagtatggt tactcataaa atttattaag aaatgtgaaa
     1441 g
//
LOCUS       DAWHSK010000322         1432 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.322, whole genome shotgun sequence.
ACCESSION   DAWHSK010000322 DAWHSK010000000
VERSION     DAWHSK010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1432
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.322"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            180..>1432
                     /gene="ribB"
                     /locus_tag="WIC03_002057"
     CDS             180..>1432
                     /gene="ribB"
                     /locus_tag="WIC03_002057"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEZ3884506.1"
                     /translation="MTDTAGLRRRNLRQWIAEHYGGLQTRFAEAVALNTGELSALLKN
                     KSFGEKKARKIEQAAKMPAFWLDTEHTARPPEHTGKHTMSHISPIPEILADIKAGKMV
                     IITDAEDRENEGDLLMAAQFVTPEAINFMIKHARGLVCLPMDGEMVEKLGLPMMTQKN
                     GAQYGTNFTVSIEAAHGITTGISAADRALTIQTAVSPTAKPEDIVQPGHIFPLRAQKG
                     GVLVRAGHTEAGVDLAQMNGLIPASVICEIINDDGTMARMPELMKFAEEHKLKIGTIA
                     DLIEYRSRTESLLEDMGNAPVQTPWGEFQQHVYVDKLSGETHLALVKGTPSADTETLV
                     RVHEPFSVMDFIQANPRHSWSLPKALERIQQAESGVVILLHRTEDGAALLDRTLPKGA
                     NQAYKWDSKSYGIGAQILAGLNVKKL"
BASE COUNT          387 a          485 c          326 g          234 t
ORIGIN      
        1 acaaacagcc cccggccggc aaggattgcg gcaatgccgt ctgaagccac gaatccggct
       61 tcagacggca tctgtttacc aaaaagcaaa taattcggtt tggcgaaaaa aacagatttg
      121 ctttttggta aatacgcgat tacaatccgc tacatccgat ttctacaaag gatgaaacga
      181 tgaccgacac agccggtctg cgccgccgca acctgcggca atggatagca gaacactacg
      241 gcggtttgca aacccgtttt gccgaagccg ttgccctcaa cacaggcgaa ctctccgccc
      301 ttttgaaaaa caaatccttc ggcgagaaaa aagcccgtaa aatcgaacag gcggcaaaaa
      361 tgcccgcctt ctggctcgat accgaacaca ccgcccgccc gcccgaacac acaggaaaac
      421 acaccatgtc ccatatttcc cccatccccg aaatcctagc cgacatcaaa gccggcaaaa
      481 tggtcatcat caccgatgcc gaagaccgag aaaacgaagg cgacctgctg atggcggcgc
      541 aattcgtcac gcccgaagcc atcaacttca tgatcaaaca cgcgcgcggc ttggtctgcc
      601 tgccgatgga cggcgaaatg gtcgaaaaac tcgggctgcc gatgatgacc caaaaaaacg
      661 gcgcgcaata cggcaccaac tttactgtct ccatcgaagc cgcacacggc attaccaccg
      721 gcatttccgc cgccgaccgc gccctgacta ttcaaaccgc cgtttccccg accgctaaac
      781 ccgaagacat cgtccaaccc ggtcatatct ttccgcttcg cgcccaaaaa ggcggcgtac
      841 tcgtccgcgc cggacacacc gaagccggcg tcgacctggc gcaaatgaac gggctgattc
      901 ctgcctccgt tatttgcgaa atcatcaacg acgacggcac gatggcgcgt atgcccgaac
      961 tgatgaaatt cgccgaagaa cacaagctca aaatcggcac gattgccgac ctcatcgaat
     1021 accgcagccg taccgaaagc ctgcttgaag acatgggcaa tgcgcctgta caaaccccgt
     1081 ggggcgagtt ccaacaacac gtttacgtcg acaaactctc cggcgaaacc cacctcgccc
     1141 tcgtcaaagg cacgccctcc gccgacaccg aaaccctcgt ccgcgtccac gaacccttca
     1201 gcgtcatgga cttcatccaa gctaacccgc gtcattcatg gtcgctgccc aaagcactcg
     1261 agcgcatcca acaggccgaa agcggcgtag tcatccttct gcaccgcacc gaagacggcg
     1321 ccgccctgct cgaccgcacc ctgcccaaag gtgcaaacca agcctacaaa tgggacagca
     1381 aaagctacgg catcggtgcg caaatcctcg ccggcctcaa cgtcaaaaaa ct
//
LOCUS       DAWHSK010000323         1432 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.323, whole genome shotgun sequence.
ACCESSION   DAWHSK010000323 DAWHSK010000000
VERSION     DAWHSK010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1432
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.323"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            128..1255
                     /locus_tag="WIC03_002058"
     CDS             128..1255
                     /locus_tag="WIC03_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEZ3884507.1"
                     /translation="MKIVFITTVASSIYGFRAPVIKKLIGKNHQVYAFVSEFSDNELD
                     IIREMGVTPVTYRSNRSGLNPFSDIKSTFLIFKELKKISPDLVFPYFAKPVIFGTFAA
                     KLAGVPRIVGMLEGLGFAFTPQPEGIPLKTKIIKGILIALYRIALPMLESLIVLNPDD
                     KDELTDKYGIKIKNIHILGGIGLDSRQYPYSEADIPDEKEPVKFLFIGRFLKEKGIDD
                     FIRAAEQVKDKYPDTVFTALGAIDKSRGGGDLERLAARDIIRFPGFVNNVSEVIKEHH
                     IFVLPSYYREGVPRSTQEAMAVGRAVITTDVPGYRETVADKVNGFLIEPWNPRILAEK
                     MIYFIENREAVRLMGNASYAIAKDKFDAEKVDLKLLDILKA"
BASE COUNT          387 a          343 c          336 g          366 t
ORIGIN      
        1 ggcaacgccg taccggtttt tgttaatcca ctataatacg caggcaaacc caagccttgc
       61 tgggttacag gatgaaaccc tgccgtagca gaccgcattt ccaatccata ttcaaggtgt
      121 ccgccttatg aaaatcgttt ttatcacaac agtcgcatcc agcatttacg gtttccgcgc
      181 ccccgtcatt aaaaaattaa tcggcaaaaa ccatcaggtg tatgcctttg tatcggagtt
      241 ttccgataat gaattggata ttatcaggga aatgggggtt acacccgtta cctaccgttc
      301 aaaccgcagc gggctgaacc cgttttccga tataaaatcc accttcctca tctttaaaga
      361 actcaaaaaa atatcgccgg atttggtttt cccttatttc gcaaaacccg tgattttcgg
      421 cacttttgcc gcaaaactgg caggcgtgcc cagaatcgtc gggatgctgg aaggtttggg
      481 attcgcattt accccgcagc cggaaggcat accgttaaaa acaaaaatca taaaggggat
      541 cttgattgcc ttataccgca ttgccctgcc gatgttggaa agcctgattg tattaaaccc
      601 cgacgacaaa gacgaactga cggacaaata cggcatcaaa ataaaaaaca tccatatttt
      661 gggcggaatc ggtctggatt cgcggcaata tccttattcc gaggcggata ttcccgatga
      721 aaaagaaccc gtaaaattcc tctttatcgg cagatttctg aaagaaaagg ggattgatga
      781 ttttattcgg gcggcggaac aggttaagga caaatacccc gatacggttt ttaccgcttt
      841 gggcgcaatc gacaaatcac gcgggggggg cgatttggaa cggcttgccg cccgcgatat
      901 tatccgtttc cccggttttg tgaacaatgt ttccgaagtg ataaaagaac atcatatatt
      961 cgtattgccg tcttattata gggaaggcgt tccccgaagc actcaggagg caatggccgt
     1021 cggcagggca gtgattacga cggatgtccc cggatacagg gaaacggtcg ccgacaaggt
     1081 caacggcttc ctgatcgaac cttggaatcc ccgcatcttg gccgaaaaaa tgatttattt
     1141 tatcgaaaac agggaagccg tccgcctgat ggggaatgca agttatgcga ttgccaaaga
     1201 taaattcgat gccgaaaaag tcgatttgaa attgctcgat attttgaagg cgtaaacaag
     1261 gctgcccgct tttgagtttc ggtcatttct gataaacccc cgtcattccc gccacacccc
     1321 cgtcattccc gcgaaagcgg gaatccaggt tcgttgagtt tcggtcattt ccgataaatt
     1381 cttgcaactt tgagtttcta gattcccact ttcgtgggaa tgacggcgga gg
//
LOCUS       DAWHSK010000324         1422 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.324, whole genome shotgun sequence.
ACCESSION   DAWHSK010000324 DAWHSK010000000
VERSION     DAWHSK010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1422
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.324"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            240..470
                     /locus_tag="WIC03_002059"
     CDS             240..470
                     /locus_tag="WIC03_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884508.1"
                     /translation="MNIVKKYAVKALLAAGIFTPAIVMADTFDAAAIGTQVANVIMGF
                     VSMVSAVGMAAITVILAIQGFKMAWSMIKSVK"
     gene            498..797
                     /locus_tag="WIC03_002060"
     CDS             498..797
                     /locus_tag="WIC03_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884509.1"
                     /translation="MGYRVGINCFDTRLQADDYLLSSLPPTVTQDGKIIRPERVGDKW
                     ILNGKPVTLSYPKCSNYEQVKSGAYLGSMVLILFVVIYGFRLLINFLKDIGKVGA"
     gene            916..1236
                     /locus_tag="WIC03_002061"
     CDS             916..1236
                     /locus_tag="WIC03_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1132 family protein"
                     /protein_id="HEZ3884510.1"
                     /translation="MNKPFITQAQLALYKYQPSSKYFGQSMALIASKEFEEFVRNVKE
                     YDVIECFSYFLNKRVTHNIWKIYFSDESNIFIRKSEENGKISHEFIYSEFSDSNTDFN
                     VLFS"
BASE COUNT          445 a          250 c          306 g          421 t
ORIGIN      
        1 aaaagaaaaa caggctcggc gggcggtctg tcaacctttc acaaagcccg caacaaagga
       61 aaaatatcat gaaaatgaac cttgcaacac taattatcgg ctgggtggtc tgtatgtttc
      121 tttttctttt cgccatcctc tattttatcg gctaaaaaca ggattcggga aagacttcgt
      181 ccggatgaag caagtcaaga agtcgtttta ctttaaacat caaaaaaagg aaaaaaacga
      241 tgaacatcgt taaaaaatat gctgtaaaag cattgttggc agccggtatc ttcacaccgg
      301 ccattgttat ggcagatacc tttgatgcag ccgcaattgg tacgcaagta gcgaatgtaa
      361 tcatgggttt cgtgtcaatg gtttccgccg tgggtatggc ggccattacc gtgattcttg
      421 caatccaagg cttcaaaatg gcttggagca tgattaaatc tgtcaaataa acagagtgaa
      481 gaaaaagggg cgcataaatg ggctatcgtg tcggcataaa ttgttttgat acaagattgc
      541 aggcagacga ctatttattg tcgtcccttc ctcctactgt tacccaggac ggaaaaatca
      601 tcaggccgga aagggtgggc gataaatgga ttttgaacgg aaagccggtc acgctgtctt
      661 atccgaaatg ttcgaattac gaacaagtta aatccggagc ttatctcggg tctatggttt
      721 taattctgtt tgtcgttatt tacggcttca ggctgctgat taatttctta aaagacatag
      781 gcaaagtagg ggcgtgatga tgtttgtcga tttttggttt ttgctcggat ttttcctagc
      841 tttgtctgtc gctttgatat ttatatgacg tgttttaaaa tcaggctttc aaaacaacct
      901 ttgaaagaca gaaacatgaa caagccgttt atcacgcagg cgcagttggc actttataaa
      961 tatcagccgt ccagcaagta ttttgggcag tcgatggcat taattgcgtc taaggaattt
     1021 gaagagtttg taagaaatgt aaaagaatac gacgtaatag aatgtttctc ttatttttta
     1081 aataagaggg taacgcataa tatttggaaa atttattttt ctgatgagtc taatattttt
     1141 attaggaagt cagaagaaaa tggaaaaatt tcgcatgaat ttatttactc ggaattttct
     1201 gatagcaaca ccgattttaa tgtgttgttc tcttagtttt gcagaaccag caagaataga
     1261 tgatcgaata ataaaattta ggccatctaa atcaaagttt tttgaatctc caggatatag
     1321 aaaaatcaat aatgaatttt ctaaattcac agaagcggca aatgtcgaac atatccccac
     1381 gggcgcaaaa gcccgaatca acgcaaagat aaccgccagc gt
//
LOCUS       DAWHSK010000325         1421 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.325, whole genome shotgun sequence.
ACCESSION   DAWHSK010000325 DAWHSK010000000
VERSION     DAWHSK010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.325"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            525..>1421
                     /locus_tag="WIC03_002062"
     CDS             525..>1421
                     /locus_tag="WIC03_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly/adherence protein PilC"
                     /protein_id="HEZ3884511.1"
                     /translation="MNAQNLPEVKWGQDYRKLAQKSNERQFTHTTNFYIKKNVTLSFN
                     NIDEVVAEKKDVVVFGTATYLPPYGKVSGFDADKLKKRGDALGWIKTIKPGLVGYSYE
                     GVTCQNNYNNASRGCPELIYKTQFSFGQQGLKKKTNGRLDIDEDKSRDNSPIYKLQDY
                     PGLGVSFNLSSESLVKSVKYNKIISSFSEDVTQQNGTQSHHKDKNLVYTTGDYQYKNR
                     YSSRYVGQDEHSAVAFYLNAKLHLLDKKNIKNIAQGKTVNLGTLKPYVEPTEEWKNKR
                     GNHFQGNWTFEDKGEVSVKLKLP"
BASE COUNT          432 a          323 c          350 g          316 t
ORIGIN      
        1 gacgggattt gagattgcgg catttatcgg aaaaaacaga aaccgctccg ccgtcattcc
       61 cgcgcaggcg ggaatccaga ccttgggata acggcaatat tcaaaggtta taaaagaccc
      121 gtcattcccg cgcaggcggg aatccggctt gttcggtttc ggtttttttt gaggtttcgg
      181 gcaacttcta aaccgtcatt cccgcgcagg cgggaatcca gaccgttggg catctgcagc
      241 ggtttgctaa aaaacgcttt accgtgatca gtgtgcaaag ttaaaatggg gaggtaagct
      301 tttcaatcag caattcggcg ggcgcggaat cgggcggttt accgaacccc ggcgttcgcg
      361 gcgcgccgcc ccgtcccgtg aaggcaaact caaggaataa aagatgaata aaacttggaa
      421 acggcaggtt ttccgccata ccgcgcttta taccgccata ttgatgtttt cccataccgg
      481 cggggggggg ggcgcaggcg caaacgcata aatacgctat tgtaatgaac gcgcaaaatc
      541 tgcctgaggt aaagtggggg caggactata gaaaattggc gcaaaaaagc aatgaacgcc
      601 aatttaccca tacgactaat ttttacatca aaaaaaacgt aactttatca ttcaataata
      661 tcgatgaagt tgttgctgaa aaaaaagatg ttgtcgtttt cggcacagcc acctacctgc
      721 cgccctacgg caaggtttct ggttttgatg ccgataagct gaaaaagcgc ggcgatgccc
      781 ttggttggat taagacgatc aaaccggggt tggtaggcta cagctacgaa ggtgtcacat
      841 gccaaaacaa ctacaacaac gccagccgcg gctgtcctga acttatctat aaaacccaat
      901 tttctttcgg tcagcaaggg ttgaaaaaaa agacaaacgg caggctggat atagacgaag
      961 acaaaagccg cgacaattcg cccatttaca aattgcagga ttatcctggg ttgggcgtgt
     1021 ctttcaattt gagcagcgag agcctcgtca aatcagtaaa atacaataaa attatatctt
     1081 cttttagcga ggacgtgacg cagcaaaatg gtacgcaaag ccaccacaaa gacaaaaacc
     1141 tcgtttatac gacaggcgat taccagtata agaatagata ttcttcccgt tatgtcgggc
     1201 aggacgaaca ctccgccgtc gctttttatc tgaacgccaa actgcacctg ctggataaaa
     1261 aaaacatcaa aaatatcgcg caaggcaaaa cagttaattt gggtaccttg aaaccgtacg
     1321 tcgagccgac ggaagaatgg aaaaacaaga gggggaacca ttttcaaggt aattggacgt
     1381 ttgaagataa aggagaagtc agcgtcaaac tcaaattgcc g
//
LOCUS       DAWHSK010000326         1375 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.326, whole genome shotgun sequence.
ACCESSION   DAWHSK010000326 DAWHSK010000000
VERSION     DAWHSK010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1375
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.326"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..1081
                     /locus_tag="WIC03_002063"
     CDS             <1..1081
                     /locus_tag="WIC03_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ3884512.1"
                     /translation="GSDTAAQAPSAKPEAEQSGKLNIYNWSDYVDPETVAAFEKETGI
                     KTRSDYYDSNETLEAKVLTGKSGYDLTAPSIANVGRQIKAGAYQKIDKAQIPHYGNID
                     KDLLKMMEAVDPGNEYAVPYFWGINTLAINTRQVQKALGTDKLPENEWDLVFKPEYTA
                     KLKSCGISYFDSAIEQIPLALHYLGKDPNSENPEDIKAAVDMMKAVRGDVKRFSSSGY
                     IDDMAAGNLCAAIGYGGDLNIAKTRAEEAANGVEIKVLTPKTGVGVWVDSFMIPRDAQ
                     NVANAHRYIDYTLRPEVAAKNGSFVTYAPASRPARELMDEKYTSDASIFPNKELMEKS
                     FIVSPKSAESVKLGVKLWQGLKAGK"
     gene            1214..>1375
                     /locus_tag="WIC03_002064"
     CDS             1214..>1375
                     /locus_tag="WIC03_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ3884513.1"
                     /translation="MDLSIVVPIYNVESYLEACLSSIESILSNENVELILVNDGSKDG
                     SEDICYKYID"
BASE COUNT          393 a          358 c          347 g          277 t
ORIGIN      
        1 cggaagcgat accgccgccc aagccccctc cgccaagccc gaagccgaac aatcgggcaa
       61 actcaatatc tacaactggt cggattatgt cgatcccgaa accgtcgccg cctttgaaaa
      121 agaaaccggc atcaagacgc gttccgatta ttacgacagc aacgaaacac tggaggcaaa
      181 agtcctgacc ggcaaatccg gctatgacct gactgcgccg tccatcgcca acgtcggccg
      241 gcaaatcaaa gcgggcgcgt atcagaaaat cgacaaggcg caaatccccc attacggcaa
      301 catcgataaa gatttgctga aaatgatgga agccgtcgat ccgggcaacg aatacgccgt
      361 cccctatttc tggggcatca acaccttggc aatcaacacg cggcaggtgc aaaaggcatt
      421 gggtacggac aagctgcccg aaaacgaatg ggatttggtg ttcaaacccg aatacaccgc
      481 caaactcaaa tcctgcggca tcagctattt cgacagtgca atcgaacaga ttcctttggc
      541 gttgcactat ttgggcaaag accccaacag tgagaatccc gaagacatca aagccgccgt
      601 cgatatgatg aaagccgtcc ggggcgacgt gaaacgcttc agttcttccg gctatatcga
      661 tgatatggcg gcgggcaacc tgtgtgccgc catcggttac ggcggcgatt tgaacattgc
      721 caaaacccgt gccgaagaag ccgcaaacgg cgtggaaatc aaagtattga ctccgaaaac
      781 cggcgtgggc gtgtgggtgg attcctttat gattccgcgc gacgcgcaaa acgtcgccaa
      841 cgcccaccgc tatatcgact acacgctccg gcccgaggtg gcggcgaaaa acggcagctt
      901 cgttacctac gcgcccgcca gccgtcccgc gcgcgagctg atggatgaaa aatacacctc
      961 cgacgcatcg attttcccga acaaagaact gatggaaaaa agtttcatcg tatcgcccaa
     1021 atccgcagaa tccgtcaaac tgggcgtgaa gctgtggcaa gggctcaaag cgggcaaata
     1081 accggaatcc ctgccgtctg aaacctttcg ggcggcagga aacggcgcgt ccgttatcaa
     1141 acaggggggc gtttcccctc ctgccggtta tgattgggtt aagattaaaa tgatttagta
     1201 aaatgagaaa gatatggatt taagtatcgt agttcctatt tataatgtcg aaagttattt
     1261 ggaagcgtgt ttaagttcca tagaatctat attaagtaat gaaaatgtcg aacttatcct
     1321 tgtgaatgac gggtcaaaag acggaagtga agatatatgt tataaatata tagat
//
LOCUS       DAWHSK010000327         1374 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.327, whole genome shotgun sequence.
ACCESSION   DAWHSK010000327 DAWHSK010000000
VERSION     DAWHSK010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.327"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(204..>1374)
                     /locus_tag="WIC03_002065"
     CDS             complement(204..>1374)
                     /locus_tag="WIC03_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T cell/B cell stimulating protein TspB"
                     /protein_id="HEZ3884514.1"
                     /translation="GYQYDPETDKFVKGYEYSNCLWYEDERRINRTYGCYGVDSSIMR
                     LMSDYSRFPEVKELMESQMYRLARPFWNWHKEELNKLSSLDWNNFVLNRCTFDWNGGD
                     CVVNKGDDYRNGANFSLSRNPKYKEEMDAKKLEEILSLKVDANPDKYIKATGYPGYSE
                     KVEVAPGTKVNMGPVTDRNGNPVQVVATFGRDSQGNTTVDVQVIPRPDLTPGSAEAPN
                     AQPLPEVSPAENPANNPNPNENPGTSPNPEPDPDLNPDANPDTDGQPGTRPDSPAVPG
                     RTNGRDGEDGGLLCKFFPDILACDRLPEPNPAEDLNLPSETVNVEFQKSGIFQNSAQC
                     PAPVTFTVTVLDSSRQFAFSFENACTIAERLRYMLLALAWAVAAFFCIRTVSREV"
BASE COUNT          296 a          335 c          364 g          379 t
ORIGIN      
        1 tgcgacgccg cgggggcagg gggaacggcg caaaaagcgc gcgccttacc acctgccctt
       61 gcggcagagt gtattctttt ggcggggcgg caaggggtat ccaaaaagat ttataaagac
      121 gataaagccg tctttacaaa tctttctgga cgtcctcctc ctgccttggt acaagttact
      181 gaagcccggc ggtgctgcgc ctgctagact tcacgggata ctgtgcggat acagaaaaat
      241 gcggcaaccg cccaagcaag ggcgagaagc atgtacctta gccgttcggc tatggtacat
      301 gcgttctcaa agctgaacgc gaactgcctg ctggaatcaa gcacagtcac tgtgaaagtg
      361 acaggtgcgg gacactgtgc ggaattttga aagattcctg atttctgaaa ctctacattg
      421 acggtttcag acggcagatt taaatcttct gccggattgg gctcgggcag cctgtcgcaa
      481 gcgagaatgt cggggaagaa tttgcacaaa aggccgccgt cttcgccgtc cctgccgttt
      541 gtgcggcccg gaactgcggg ggaatcgggt cttgtgccgg gctgtccgtc cgtatcggga
      601 tttgcatcgg gattcaaatc ggggtcgggt tcgggattgg ggctcgtgcc ggggttctca
      661 ttggggttcg ggttgtttgc ggggttttcg gcgggcgata cttcgggcag cggctgtgcg
      721 ttcggtgctt ccgcgcttcc gggtgtgagg tcgggacgcg ggattacttg aacatccact
      781 gtggtgttgc cttgcgaatc cctgccgaat gttgcgacaa cctgaacggg attcccgttc
      841 ctgtccgtaa caggccccat attcactttt gttccgggtg ctacttctac tttttcggaa
      901 taaccgggat atccggttgc ctttatgtat ttgtcgggat tggcatcgac tttcaacgat
      961 aaaatctctt ccagcttttt ggcatccatt tcttctttgt atttcggatt gcggctaagg
     1021 gaaaaattag ccccatttct gtaatcatca cctttattga ccacacaatc tccgccgttc
     1081 caatcaaatg tgcaacgatt taaaacaaaa ttattccaat ccaaagaact taatttattc
     1141 agttcttctt tatgccaatt ccaaaacgga cgtgccagcc tatacatttg gctttccatc
     1201 aattctttga cttcggggaa tctgctgtaa tcggacataa ggcgcataat cgaactgtca
     1261 acgccgtagc agccataggt tctattaata cgtctttcgt cttcatacca aaggcaatta
     1321 ctatattcgt agccttttac aaatttgtcg gtttcggggt cgtattggta gcct
//
LOCUS       DAWHSK010000328         1350 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.328, whole genome shotgun sequence.
ACCESSION   DAWHSK010000328 DAWHSK010000000
VERSION     DAWHSK010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.328"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..630
                     /locus_tag="WIC03_002066"
     CDS             <1..630
                     /locus_tag="WIC03_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEZ3884515.1"
                     /translation="SDGQFVQNERIGGLWYGAVMPEQWGGKTRNADFYDIKADVENLL
                     KNKAVEFVKTGHPALHPGRAANIVSDGKVIGFVGELHPKWLQKYDLPQAPLVFEIDMA
                     AVLECGKTRYRVVSKFQPVRRDLAFVMPEAMSHDDLLLVLKGAANKLVQEISVFDVYR
                     GTGLPEGMKSVAVKVILQDMENTLTDEAVEPLIGKLIGAATAAGARLRS"
     gene            704..1006
                     /locus_tag="WIC03_002067"
     CDS             704..1006
                     /locus_tag="WIC03_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002641098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HEZ3884516.1"
                     /translation="MTLTKAELADILVDKVSNVTKNDAKEIVELFFEEIRSTLASGEE
                     IKISGFGNFQLRDKPQRPGRNPKTGEEVPITARRVVTFHASQKLKSMVEHYYDKQR"
     gene            990..1298
                     /locus_tag="WIC03_002068"
     CDS             990..1298
                     /locus_tag="WIC03_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884517.1"
                     /translation="MTNNVDLKVPAKRYFTLDELCGLLQISPYGFAQWQHDHGVVVGY
                     GGERYTRLDVVKLLKLQSTFAPYAEGAESGSDGNRPVTLQEIGDGLKELLADLDKELC
                     "
BASE COUNT          363 a          302 c          386 g          299 t
ORIGIN      
        1 tcagacggcc agtttgtcca aaacgaacgc atcggcggat tgtggtacgg cgcggtcatg
       61 ccggaacaat ggggcgggaa aacgcgcaat gcggattttt acgacatcaa ggcggacgtg
      121 gaaaatctgt tgaaaaacaa agcagtcgag ttcgttaaaa ccggacatcc cgccctgcat
      181 cccggacgtg ccgccaatat cgtttcagac ggcaaagtca tcggctttgt cggcgaactg
      241 catccgaaat ggctgcaaaa atacgacctg ccgcaagcgc cgctggtatt tgaaatcgat
      301 atggcggccg tgttggaatg cgggaaaacg cgctatcggg tcgtatcgaa attccagccg
      361 gtgcgccgcg atttggcgtt tgtgatgccg gaagctatga gccatgatga tttgctgctt
      421 gtcttgaaag gcgcggcaaa caagttggta caggaaatca gcgtgtttga cgtgtatcgc
      481 ggcacgggac tgcccgaagg gatgaagagc gtggcggtca aagtgatttt gcaggatatg
      541 gaaaacacgc tgacggatga ggcagtcgag ccgcttatcg gaaaactgat tggcgcggca
      601 acggcggcag gggcgcggct tcgcagttaa aaaataaata ttcgcttgaa ttttaaataa
      661 aaattggtaa taatccacaa ctgttacaac agaaggtaat catatgactc tcactaaagc
      721 agaactggcc gatattttgg tagacaaagt cagcaacgtc accaaaaacg atgccaaaga
      781 aatcgtcgaa ctcttttttg aagaaatccg cagcactttg gcaagcggcg aagaaatcaa
      841 aatttccggt ttcggaaatt tccagttgcg cgacaagccg caacgcccgg gtcgtaatcc
      901 taaaaccggc gaggaagtgc cgattaccgc ccgccgcgtg gtaactttcc atgccagcca
      961 gaaactcaaa agcatggtgg aacactacta tgacaaacaa cgttgatttg aaggttcccg
     1021 caaaacgcta tttcacgctg gacgagttgt gcggactgtt gcaaatcagc ccctatggtt
     1081 ttgcgcaatg gcagcatgat cacggtgtgg ttgtcggtta cggcggcgaa cgctacaccc
     1141 gtttggatgt ggtgaaactg ttgaaattgc agagcacgtt tgcaccgtat gcagaaggtg
     1201 cggagtcggg ttcggacggc aaccgtccgg ttacgcttca ggaaatcgga gacggtctga
     1261 aagagctgtt ggcggatttg gataaggaat tgtgctgatt tgaggccggt tgcaggtatg
     1321 cagccggttt tgttttacac gctaaaaaat
//
LOCUS       DAWHSK010000329         1343 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.329, whole genome shotgun sequence.
ACCESSION   DAWHSK010000329 DAWHSK010000000
VERSION     DAWHSK010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.329"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..>1343
                     /locus_tag="WIC03_002069"
     CDS             <1..>1343
                     /locus_tag="WIC03_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HEZ3884518.1"
                     /translation="EKLSKQPEYAYLKQLQTAKNVDWKQVQLAYDKWDYKQEGLTGAG
                     AAIVVIIVTALTYGYGAAAAGSVTAAGSSTAAAATTTAAATTTVSTAAAMQTAALASL
                     YSQAAVAIINNKGDVGKALKDLGTSDTVKQIVTSALTAGALNQMGADIAQLNSKVRTE
                     LFSSTGNQTIANLGGRLATNLSNAGISAGINTAVNGGSLKDNLGNAALGALVNSFQGE
                     AASKIKTTFSDDYVAKQFAHALAGCVSGLVQGKCKDGAIGAAVGEIVAESILGGRNPA
                     TLSDAEKHKVISYSKIIAGSVAALNGGDVNTAANAAEVAVVNNALNFDSTPTNAKKHQ
                     PQKPDKTALEKIIQGIMPAHAAGAMTNPQDKDAAIWISNIRNGITGPIVITSYGVYAA
                     GWTAPLIGTAGKLAISTCMANPSGCTVMVTQAAEAGAGIATGAVTVGNAWEAPVGA"
BASE COUNT          378 a          322 c          371 g          272 t
ORIGIN      
        1 gaaaagctgt ccaaacagcc cgaatacgcc tacctgaaac agcttcagac ggccaagaac
       61 gtcgattgga aacaggtgca gctggcctac gacaaatggg actataaaca ggaaggcttg
      121 accggagccg gtgcagcgat tgtggtgatt attgtaaccg ctctaactta tggatacgga
      181 gcggctgcag cgggtagcgt aactgccgca ggaagtagta cagccgcagc tgcaacaacg
      241 acagcggcag caactaccac tgtttctact gcagctgcca tgcaaaccgc agctttagcc
      301 tccttgtata gccaagcagc tgtagccatc atcaataata aaggcgatgt aggcaaagca
      361 ttaaaagatc tcggcaccag tgatacggtc aagcagattg ttacttccgc actgacggcg
      421 ggtgcattaa accagatggg agcagatatt gcccaattga acagcaaggt aagaaccgaa
      481 ctgttcagca gtacgggcaa tcaaaccatt gccaaccttg gaggcagatt ggccaccaac
      541 ctcagcaatg caggtatctc agctggtatc aataccgccg ttaacggcgg cagcttgaaa
      601 gacaacttag gcaatgccgc attgggagca ttggttaata gcttccaagg ggaagccgcc
      661 agcaaaatca aaacaacctt cagcgacgat tatgttgcca aacagttcgc ccacgctttg
      721 gcgggttgtg ttagcggact ggtacaggga aaatgtaaag atggggcaat tggcgcagca
      781 gttggggaaa tcgtagctga atccatactt ggcggcagaa accctgctac actcagcgat
      841 gcagaaaagc ataaagttat cagttactcg aagattattg cgggcagcgt ggcggcactc
      901 aacggcggcg atgtgaatac tgcggcgaat gcggctgagg tagctgtggt gaataatgct
      961 ttgaattttg acagtacccc taccaatgcg aaaaagcatc aaccgcagaa gcccgacaaa
     1021 accgcactgg aaaaaattat ccaaggtatt atgcctgcac atgcagcagg tgcgatgact
     1081 aatccgcagg ataaggatgc tgccatttgg ataagcaata tccgtaatgg catcacaggc
     1141 ccgattgtga ttaccagcta tggggtttat gcagcaggtt ggacagctcc gctgatcggt
     1201 acagcgggta aattagctat cagcacctgc atggctaatc cttctggttg tactgtcatg
     1261 gtcactcagg ctgccgaagc gggcgcggga atcgccacgg gtgcggtaac ggtaggcaac
     1321 gcttgggaag cgcctgtggg ggc
//
LOCUS       DAWHSK010000330         1340 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.330, whole genome shotgun sequence.
ACCESSION   DAWHSK010000330 DAWHSK010000000
VERSION     DAWHSK010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.330"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..435
                     /locus_tag="WIC03_002070"
     CDS             <1..435
                     /locus_tag="WIC03_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HEZ3884519.1"
                     /translation="KQAIPTQTVKELDGLLQESKNIGAVNTRINIANSTTRYTPMRQT
                     GQPVSAGFEHVLEGHFHRPIANNRSVFTISPNELKVILQSNKVVSSPVSMTPDGQYMR
                     TVDVGKVIGTTSIKEGGQPTTTIKVFTDKSGNLITTYPVKGN"
     gene            442..951
                     /locus_tag="WIC03_002071"
     CDS             442..951
                     /locus_tag="WIC03_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884520.1"
                     /translation="MSNFEKKYILELNDALSHLNHNSTSFDLLKVLISWLSNDIVIDK
                     FKILGYDFSKYIEMNPDDYPVEKSILNREEIIYLKNNIYRKISSGNFKFQYFVQYIRD
                     ILEYLFIEHIERVCPYCEWGEMQKLEEQNTHETVYLCTQCGCAFYNDNSQFLLKTPLT
                     IPMKRDEFK"
     gene            1020..>1340
                     /locus_tag="WIC03_002072"
     CDS             1020..>1340
                     /locus_tag="WIC03_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HEZ3884521.1"
                     /translation="MIYGSMPSEKLTIFQTAFVMQVNIQIPCMLYRRGSVKPPLFEAP
                     RLLPSFTDPVVPKLSAPGGYIVDIPKGNLKTEIEKLAKQPEYAYLKQLQVAKNVNWNQ
                     VQLAY"
BASE COUNT          455 a          259 c          239 g          387 t
ORIGIN      
        1 aagcaggcta taccaaccca gacagttaaa gaacttgatg gcttactaca agaatcaaaa
       61 aatataggtg ctgtaaatac acgaattaat atagcgaata gtactactcg atatacacca
      121 atgagacaaa cgggacaacc ggtatctgct ggctttgagc atgttcttga ggggcacttc
      181 cataggccta ttgcgaataa ccgttcagtt tttaccatct ccccaaatga attgaaggtt
      241 atacttcaaa gtaataaagt agtttcttct cccgtatcga tgactcctga tggccaatat
      301 atgcggactg tcgatgtagg aaaagttatt ggtactactt ctattaaaga aggtggacaa
      361 cccacaacta caattaaagt atttacagat aagtcaggaa atttgattac tacataccca
      421 gtaaaaggaa actaactaaa tatgagtaac tttgaaaaaa aatatatttt agaattaaat
      481 gatgctttaa gccatttaaa tcataactct acctcatttg atttattgaa agttttgatt
      541 tcatggttat caaacgatat tgtcattgat aaatttaaaa ttttaggtta tgactttagt
      601 aaatatatcg aaatgaatcc cgatgactat ccggttgaaa aatctatatt gaatagagag
      661 gaaattattt atctcaaaaa caatatttat cgtaaaatat catcaggaaa ttttaaattt
      721 caatactttg tacaatatat tagagatatt ttagaatatt tatttattga acatattgaa
      781 agagtctgtc cttactgcga atggggtgaa atgcaaaaat tagaagaaca aaatacgcat
      841 gaaacggtgt atctctgtac tcaatgtgga tgtgcttttt ataacgataa ttcacaattt
      901 ttattaaaaa cccctttaac cattccaatg aaacgtgatg aatttaaata aacaagccgt
      961 agcctgcatg aaccctaaaa tccacgtgta gcgtgtgtgc gccagcacgc atgcgttcca
     1021 tgatttacgg ctcaatgccg tctgaaaagc tcacaatttt tcagacggca tttgttatgc
     1081 aagtaaatat tcagattccc tgtatgctgt acagacgcgg gagtgttaag ccccccttgt
     1141 ttgaagctcc gcggctcctg ccgagcttca ccgaccccgt tgtgcccaag ctctctgctc
     1201 ccggcggcta cattgtcgac atccccaaag gcaatctgaa aaccgaaatc gaaaagctgg
     1261 ccaaacagcc cgagtatgcc tatctgaaac agctccaagt agcgaaaaac gtcaactgga
     1321 accaggtgca actggcttac
//
LOCUS       DAWHSK010000331         1326 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.331, whole genome shotgun sequence.
ACCESSION   DAWHSK010000331 DAWHSK010000000
VERSION     DAWHSK010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.331"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..363)
                     /locus_tag="WIC03_002073"
     CDS             complement(<1..363)
                     /locus_tag="WIC03_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysO family transporter"
                     /protein_id="HEZ3884522.1"
                     /translation="MDSLMTLISVLIPMFAGFFIRVPKPYLPALDKVLSVLVYAVLLL
                     IGVSLSRVEDLGSRLDDMALTVLWLFVCTVGTNLLALAVLGKLFPWRIKGKGKGVSVG
                     VSGSVGQLGCVLLGFASGK"
     gene            complement(512..1228)
                     /locus_tag="WIC03_002074"
     CDS             complement(512..1228)
                     /locus_tag="WIC03_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEZ3884523.1"
                     /translation="MNFENDDIIHAPTTSSLILEERHDSELFRVYALILDGITDQVLL
                     PGKKLTESELCRQMVCSRNTVRGALSLLAHDKIVDLQPNRGAFVHVPDLKEMQDVFNA
                     RIEMETMILNILADLPDLETRLKPLYAMIRREEEASGRGDRVGWNRLSNAFHVELARL
                     VGNDVLFDIMNTLCARSSLIVAVAGVHREEKHAINTHTHSEHREILDLLLAGRRNRVV
                     KILRRHLGNCMERLEKTLED"
BASE COUNT          333 a          387 c          315 g          291 t
ORIGIN      
        1 tttgccggat gcaaatccga gcagcacgca tccgagctgc cccacactgc ccgacacacc
       61 gaccgaaacg cccttccctt tcccctttat ccgccacggg aataactttc ccaacactgc
      121 caaagcaagc aggttcgtcc cgaccgtaca aacaaacagc cacagaaccg tcaacgccat
      181 atcgtccaac cgcgaaccca aatcctccac gcgcgacaac gatacgccga tcagcagcag
      241 cacggcatac accaaaaccg atagcacctt atccaaagcg ggcaggtaag gcttgggcac
      301 acggataaaa aatccggcaa acatcggtat caaaaccgaa atcaatgtca tcaggctgtc
      361 catctcctga cctcctttat tgccgcatga tatgtgcggt ttaaaaattg ccgtctgaaa
      421 attgcagata cccgcatcca tatttcagac ggcatcaggt tcgccattaa aaaaccgcct
      481 gaaggttcag gcggcttatc cgctccggca ttcaatcttc caaagtcttt tccaaacgct
      541 ccatacagtt gcccaaatgg cggcgcagga ttttgaccac gcggttgcgc ctgcccgcca
      601 gcagcaggtc gaggatttcg cggtgttctg aatgcgtatg cgtattgatg gcgtgttttt
      661 cctcgcgatg cacgcccgcc acggcgacaa tcagggaaga ccgcgcgcac agcgtgttca
      721 taatgtcgaa cagcacatcg ttgcccacca ggcgcgccag ttcgacgtgg aaggcattgg
      781 acaggcggtt ccagccaacg cggtcgcccc tgccggaggc ctcttcttcg cgccgtatca
      841 tcgcataaag cggcttgagg cgcgtttcca aatccggcaa atctgcgagg atattcaaaa
      901 tcatcgtctc catttcgatg cgcgcattga acacatcctg catttctttc aaatcgggaa
      961 cgtggacgaa cgcgcccctg ttgggttgca aatcgacaat cttgtcgtgc gccaaaagcg
     1021 acagcgcgcc gcggacggtg ttgcgcgaac acaccatctg acggcaaagt tcggattcgg
     1081 tcagcttttt gccgggcagc agcacctgat cggtaatgcc gtccaaaatc agggcgtaaa
     1141 cacggaacag ctcagaatcg tgccgctctt cgagaatcag ggaagacgta gtcggcgcat
     1201 ggataatgtc gtcgttttca aagttcatga tgttttccgt atttttacgc tttcaaattt
     1261 tttaagatat tttaaggcgg ctgtgtttca aatcgtgtca gaggaattaa agcattgcac
     1321 aaattt
//
LOCUS       DAWHSK010000332         1325 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.332, whole genome shotgun sequence.
ACCESSION   DAWHSK010000332 DAWHSK010000000
VERSION     DAWHSK010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.332"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..159
                     /locus_tag="WIC03_002075"
     CDS             <1..159
                     /locus_tag="WIC03_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884524.1"
                     /translation="ALKFDQLILEFPERGDGAWVHIGFRRNSPQRNQILTATKKNGKT
                     VYLPGLHP"
     gene            171..680
                     /locus_tag="WIC03_002076"
     CDS             171..680
                     /locus_tag="WIC03_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884525.1"
                     /translation="MDILLKYWKPVGVLLLIVLIFTAWHFDRAEKYRMGREAAAAEIS
                     NRLKDGYIEQAKQARSAEQKAAAAFAERQTKLEEEKQNAEKTVAAMRLELNRLRHYAA
                     AQNRSRNLPATTTAATASDGASDSQGWLLFGQCAEKYAGLAETADGHAADLREWQAYG
                     HAVSSQRAE"
     gene            complement(733..1080)
                     /locus_tag="WIC03_002077"
     CDS             complement(733..1080)
                     /locus_tag="WIC03_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEZ3884526.1"
                     /translation="MYIPDKGDIFHLNFDPSGGKEIKGGRFALALSPKAFNRATGLVF
                     ACPISQGNAAAARSGGMISTLLGAGTETQGNVHCHQLKSLDWQIRKASFKETVPDYVL
                     DDVLARIGAVLFD"
     gene            complement(1082..1318)
                     /locus_tag="WIC03_002078"
     CDS             complement(1082..1318)
                     /locus_tag="WIC03_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEZ3884527.1"
                     /translation="MLRVQKWGNSAAVRLPADMLKQLDFKIGDALVAEVHNGELRVRA
                     ARRFRLADLLAEMEETPPRVEGWEILDDVGNEVV"
BASE COUNT          340 a          363 c          344 g          278 t
ORIGIN      
        1 gcgctgaaat tcgaccagtt gatactcgaa tttcccgagc gcggggacgg cgcatgggta
       61 cacatcggtt tccgacgcaa cagcccgcaa cgcaaccaga tactgaccgc aaccaagaaa
      121 aacggcaaaa ccgtgtatct gcccgggctg catccttgag gtcatctgaa atggatattt
      181 tattgaaata ctggaagccg gtaggcgtat tgctgctgat agtcctgatt tttaccgcat
      241 ggcatttcga ccgtgccgaa aaataccgca tgggacggga ggctgctgct gccgaaattt
      301 cgaatcgtct gaaagacggc tatatcgagc aggcaaagca ggcgcgttct gccgagcaga
      361 aggccgctgc cgcgtttgcc gaacgacaaa ccaaattaga agaggaaaaa caaaatgctg
      421 aaaaaactgt tgccgctatg cgtcttgagc ttaaccgcct gcgccactac gccgccgccc
      481 aaaatcgcag cagaaacctg cccgcaacca ctaccgccgc caccgcatct gatggcgcgt
      541 cagattccca aggctggcta ttattcggac agtgcgctga aaaatatgca ggactggcag
      601 aaacagccga cggacatgca gccgatttaa gggaatggca ggcatatggt catgcagtaa
      661 gcagtcagcg tgccgaataa gcaaccgccc gaacctgtaa gaaaagatta caggttcggg
      721 cggtttcagc atttaatcga ataagacggc gccgatgcgc gccagcacat cgtccaatac
      781 ataatcgggt acagtttctt taaaagaagc cttgcggatt tgccagtcca gagatttgag
      841 ctggtggcag tggacattgc cctgcgtttc cgttcctgca ccgagtaagg ttgaaatcat
      901 gccgccgctt cgtgcagccg ctgcattccc ctgtgaaatg gggcaggcaa aaaccaatcc
      961 cgttgcgcgg ttgaatgctt ttggagacag agccagcgca aaccgcccgc ccttgatttc
     1021 cttgccgccg gaagggtcga aattcaaatg gaaaatatcg cctttgtcgg gaatatacat
     1081 ttcagacgac ctcgttgccg acatcatcca agatttccca gccttctacg cgcggcgggg
     1141 tttcttccat ttcggcaagc aagtctgcca agcggaaacg tcgggcagca cgcacacgga
     1201 gttcgccgtt atgtacttcc gctaccaaag cgtcgccgat tttaaaatcc aattgtttca
     1261 gcatgtcggc aggcagtcgg acggccgccg agttccccca tttttggaca cgcaacataa
     1321 tcttc
//
LOCUS       DAWHSK010000333         1294 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.333, whole genome shotgun sequence.
ACCESSION   DAWHSK010000333 DAWHSK010000000
VERSION     DAWHSK010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.333"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..1219
                     /gene="lysS"
                     /locus_tag="WIC03_002079"
     CDS             <1..1219
                     /gene="lysS"
                     /locus_tag="WIC03_002079"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEZ3884528.1"
                     /translation="LNNKGVSQEVLDDFNHWDLGDIVGAEGTLFKTNHGELTVRVSDI
                     RLLSKSLRPLPDKHKGLSDQETKYRQRYVDLIANGESRNTFIKRSQIIQSVRNFMVGE
                     HYLEVETPMMHPIPGGATAKPFVTHHNALDIPLYLRIAPELYLKRLVVGGLERVFEIN
                     RSFRNEGMSVRHNPEFTMIEFYEAFSDYERMMQMAEDIIRNASRTVNGTAKISYNGKE
                     VDLESPFERLTILEAIKKYNPHYTDEQLNDAEWLKKEIVKHGESLPPSPGIGSLQLAL
                     FEGCAEGKLWNPTFIVDYPVEVSPLARASDTKQGLTERFELFVVGRELANGYSELNDP
                     EDQAERFKAQVAQKDAGDDEAMHYDADYIRAMEFGLPPTGGCGIGIDRLVMLLTDSQT
                     IRDVILFPQMRPE"
BASE COUNT          343 a          365 c          325 g          261 t
ORIGIN      
        1 tctgaacaac aaaggcgtga gccaagaagt tttggacgac ttcaaccatt gggatttggg
       61 cgacatcgtc ggcgcggaag gcactttgtt caaaaccaac cacggcgaac tgaccgtacg
      121 cgtgtccgac atccgcctgt tgtccaaatc cttgcgtccg ctgccggaca aacacaaagg
      181 cttgagcgat caggaaacca aataccgcca acgctatgtt gatttgattg ccaacggaga
      241 atcgcgcaat accttcatca aacgcagcca aatcatccaa tccgtgcgta attttatggt
      301 gggcgagcat tatctcgaag tcgaaacccc gatgatgcac ccgattcccg gcggcgcgac
      361 ggcaaaaccc ttcgttaccc accacaatgc cttggacatt ccgctctacc tgcgcatcgc
      421 ccccgagctg tatctgaaac gcttggttgt cggtggtttg gaacgcgtgt ttgaaatcaa
      481 ccgcagcttc cgcaacgaag gtatgtctgt gcgccacaat cccgaattta cgatgattga
      541 attttacgaa gccttctccg actacgaacg catgatgcaa atggcggaag acatcatccg
      601 caacgcatcg cgcacggtaa acggcacggc aaaaatcagc tacaacggca aagaagtcga
      661 tttggaaagc ccgtttgaac gcctgaccat tctcgaagcc atcaaaaaat acaatccgca
      721 ctacaccgac gagcagttga acgatgcgga atggctgaaa aaagaaatcg tcaaacacgg
      781 cgaaagcctg ccgccgtccc cgggcatcgg cagcctgcaa ctcgcgctgt ttgaaggttg
      841 cgccgaaggc aaactgtgga acccgacctt catcgtcgat tatccggtcg aagtttcccc
      901 gttggcacgt gcttccgata ccaaacaagg tctgaccgaa cgattcgagc ttttcgttgt
      961 cggccgcgaa ctggcaaacg gctattccga gttgaacgac cccgaagacc aagccgaacg
     1021 cttcaaagcg caagtggcgc aaaaagacgc gggcgacgac gaagccatgc actacgatgc
     1081 cgactacatc cgcgcgatgg aattcggtct gccgccgaca ggcggctgcg gcatcggtat
     1141 cgaccgctta gtgatgctgc tgaccgattc gcaaaccatc cgcgatgtga ttctgtttcc
     1201 gcagatgcgt cccgagtaat cataaaaaca gttgaaatag aaatgccgtc tgaacccgat
     1261 tggattcaga cggcattttg tatggcagga ttga
//
LOCUS       DAWHSK010000334         1231 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.334, whole genome shotgun sequence.
ACCESSION   DAWHSK010000334 DAWHSK010000000
VERSION     DAWHSK010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.334"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..716
                     /locus_tag="WIC03_002080"
     CDS             <1..716
                     /locus_tag="WIC03_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEZ3884529.1"
                     /translation="GDFRSRDLAAGGQGAPLVPAFHKALFRDDKETRVVLNIGGIANI
                     SVLPSDAPAFGFDTGPGNMLMDAWTQAHWQLPYDKNGAKAAQGNILPQLLDRLLAHPY
                     FAQPHPKSTGRELFALNWLETYLDGGENRYDVLRTLSRFTAQTVCDAVSHAAADARQM
                     YICGGGIRNPVLMADLAEYFGTRVSLRSTAELKLDPQWVEAAAFAWLAACWINRIPGS
                     SHKATGASKPCILGAGYYY"
     gene            835..>1231
                     /gene="mafA"
                     /locus_tag="WIC03_002081"
     CDS             835..>1231
                     /gene="mafA"
                     /locus_tag="WIC03_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HEZ3884530.1"
                     /translation="MKTLLLLIPLVLTACGTLTGIPAHGGGKRFAVEQELVAASSRAA
                     VKEMDLSALKGRKAALYVSVMGDQGSGNISGGRYSIDALIRGGYHNNPESATRYSYPA
                     YDTTATTKSDALSSVTTSTSLLNAPSAALT"
BASE COUNT          281 a          423 c          294 g          233 t
ORIGIN      
        1 tcggcgactt ccgcagccgc gaccttgcgg ccggcggaca aggtgcaccg ctcgtccccg
       61 cctttcacaa agccctgttc cgcgatgaca aagaaacacg cgtggtactg aacatcggcg
      121 ggattgccaa catcagcgta ctcccctccg acgcacccgc cttcggcttc gacacagggc
      181 cgggcaatat gctgatggac gcgtggacgc aggcacactg gcagcttcct tacgacaaaa
      241 acggtgcaaa ggcggcacaa ggcaacatat tgccgcaact gctcgacagg ctgctcgccc
      301 acccgtattt cgcacaaccc caccctaaaa gcacggggcg cgaactgttt gccctaaatt
      361 ggctcgaaac ctaccttgac ggcggtgaaa accgatacga cgtattgcgg acgctttccc
      421 gttttaccgc gcaaaccgtt tgcgacgccg tctcacacgc agcggcagat gcccgtcaaa
      481 tgtacatttg cggcggcggc atccgcaatc ctgttttaat ggcggatttg gcggaatatt
      541 tcggcacacg cgtttcctta cgcagcaccg ccgaactgaa gctcgatccg caatgggtgg
      601 aagcagccgc atttgcgtgg ttggcggcgt gttggattaa tcgcattccc ggtagttcgc
      661 acaaagcaac cggcgcatcc aaaccgtgta ttctgggcgc gggatattat tattgagcgc
      721 aaatgccgtc tgaaagcccg ttccgccgtt cagacggcat tttcgcccct ttcgtttaca
      781 aacccttaaa atccatttac actcaaaccc gttcaaaaca aaacaaaccc tgccatgaaa
      841 accctgctcc tcctcatccc cctcgtccta accgcctgcg gcacactgac cggcataccc
      901 gcccacggcg gcggcaaacg ctttgccgtc gaacaagaac tcgtcgccgc atcgtcccgc
      961 gccgccgtca aagaaatgga tttgtccgcc ctgaaaggac gcaaagccgc cctttacgtc
     1021 tccgttatgg gcgaccaagg ttcgggcaac ataagcggcg gacgctactc tatcgacgca
     1081 ctgatacgcg gtggctacca caacaacccc gaaagtgcca cccgatacag ctaccccgcc
     1141 tacgacacta ccgccaccac caaatccgac gcgctctcca gcgtaaccac ttccacatcg
     1201 ctcttaaacg ccccctccgc cgccctgact a
//
LOCUS       DAWHSK010000335         1226 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.335, whole genome shotgun sequence.
ACCESSION   DAWHSK010000335 DAWHSK010000000
VERSION     DAWHSK010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.335"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..81
                     /locus_tag="WIC03_002082"
     CDS             <1..81
                     /locus_tag="WIC03_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEZ3884531.1"
                     /translation="AGVAESGFALKEEHVVLTGICKKCQK"
     gene            69..995
                     /locus_tag="WIC03_002083"
     CDS             69..995
                     /locus_tag="WIC03_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEZ3884532.1"
                     /translation="MSEMKKTKVHLISGFLGTGKTTALKSLMAQKDPNEKWVIIVNEF
                     GEIGIDGAVLSDNGIPVAEIAGGCLCCTAGPQMGVTVQKMLRDAKPDRLMIEASGLAH
                     AASVIDELKAKPLDSLLEIGAVFTVVNPRQFINPDYAQQALYKDQIGICDVLVASKTD
                     LCTTEQLAEFHDKAAKLFPPKAKVVEVQNAQLDIQWLDIPVIEKSRYRLKALPDNTMG
                     FQSQGFTFPAGRDFDGEKLTNFFNDLPNMTEGLVRAKGVFQVLGTWVWLNWVDGQWGA
                     NQVSWRRDSRFELIAKSFDADLIEQKLKDALE"
     gene            1060..>1226
                     /locus_tag="WIC03_002084"
     CDS             1060..>1226
                     /locus_tag="WIC03_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEZ3884533.1"
                     /translation="MKPKFKTVLTALLLAASLPSMAATHVLMETDMGNIRLVLDESKA
                     PKTVANFVRYAR"
BASE COUNT          330 a          328 c          315 g          253 t
ORIGIN      
        1 gcaggcgttg ccgaaagcgg ctttgcgctg aaagaagaac acgttgtttt aaccgggatt
       61 tgtaaaaaat gtcagaaatg aaaaaaacca aagtccacct gatttcaggt tttctgggaa
      121 caggcaaaac caccgcgctc aaaagcctga tggcgcaaaa agacccgaac gaaaaatggg
      181 tcatcatcgt caacgagttc ggcgaaatcg gcattgacgg cgcggtattg agcgacaacg
      241 gcatccctgt ggcagaaatc gccggcggct gtttgtgttg caccgccggc ccgcaaatgg
      301 gcgtaaccgt gcagaaaatg ctgcgcgacg ccaagcccga ccgcctgatg attgaagcaa
      361 gcggactggc tcacgcagcc agcgtcatcg acgaactcaa agccaaaccg ctggacagcc
      421 ttttggaaat cggcgccgtc tttaccgtcg tcaatccgcg ccagttcatc aaccccgatt
      481 acgcgcaaca ggcgttgtat aaagaccaaa tcggcatctg cgacgtattg gtcgccagca
      541 aaaccgattt atgcaccacc gaacagcttg ccgaatttca cgacaaagcg gcaaaactgt
      601 tcccgcccaa ggctaaagtg gtcgaagttc aaaacgcaca actcgatatc caatggcttg
      661 acatccccgt catcgaaaaa tcacgctacc gcctcaaagc cctgccggac aacacgatgg
      721 gcttccagtc gcaaggtttc acattccccg ccggacgcga tttcgacggt gaaaaattga
      781 ccaacttctt caatgatttg cccaatatga ccgaaggact cgtccgtgcc aaaggcgtgt
      841 tccaagtatt gggaacgtgg gtgtggctca actgggtgga cgggcaatgg ggtgcgaacc
      901 aagtgtcttg gcggcgcgac tcgcgttttg aattgattgc caaatcgttt gatgcggatt
      961 taatcgaaca aaagctcaaa gacgcattgg aatagcgttc acgcgccttc agacggcata
     1021 tgaaatgaaa atgccgtctg aaggtctgag gaggacggga tgaaacccaa attcaaaacc
     1081 gttttaaccg cgctgctttt ggcggcttcc ctgccgtcta tggcggcaac ccatgttttg
     1141 atggaaaccg atatgggcaa tatccgtttg gttttggacg aatccaaagc ccccaaaacc
     1201 gttgccaatt tcgtgcgcta tgcccg
//
LOCUS       DAWHSK010000336         1223 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.336, whole genome shotgun sequence.
ACCESSION   DAWHSK010000336 DAWHSK010000000
VERSION     DAWHSK010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1223
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.336"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..890
                     /locus_tag="WIC03_002085"
     CDS             <1..890
                     /locus_tag="WIC03_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEZ3884534.1"
                     /translation="TAQKEAAPAQQQGGIRFSDGIAAVADNEVITRRRLAEAVAEAKA
                     NLPKEAQISESELSRQVLMQLVNQSLIVQAGKRRNIQASEAEIDAVVAKNSALKNLSP
                     AQRRDFADNIIAEKVRQQAVMQNSRVSEAEIDAFLEQAQKQGITLPEGAPLRQYRAQH
                     ILIKADSENAAVGAESTIRKIYGEARSGTDFSSLARQYSQDASAGNGGDLGWFADGVM
                     VPAFEEAVHALKPGQVGAPVRTQFGWHIIKLNEVRDAGTPQERIRNSVRQYIFQQKAE
                     QATVNLLRDLHSGAYVDIR"
BASE COUNT          328 a          365 c          317 g          213 t
ORIGIN      
        1 ccacggcgca aaaagaagcc gcacccgcac aacagcaggg cggtatccgc ttttcagacg
       61 gcattgccgc cgttgccgac aacgaagtca tcacgcgccg ccggcttgcc gaagccgttg
      121 ccgaagccaa agccaacctg cccaaagagg cgcagataag cgaatccgag ctgtcccgac
      181 aggtgctgat gcagcttgtc aaccaatccc tgattgtaca ggcgggcaaa cgccgcaaca
      241 ttcaagcaag cgaagcggaa atcgatgccg tcgtcgcaaa aaattccgcc ctcaaaaacc
      301 tcagccccgc ccaacgccgc gattttgccg acaacatcat tgccgaaaaa gtccgccagc
      361 aggcagtgat gcagaacagc cgcgtgagcg aagctgaaat cgatgccttc ctcgagcagg
      421 cgcaaaaaca aggcatcacc ctgcccgaag gcgcaccgtt gcgccaatac cgcgcccaac
      481 acatcctgat taaagccgac agcgaaaacg ccgccgtcgg cgcggaaagc accatccgca
      541 aaatctacgg agaggcccgc agcggcacag acttttccag cctagcgcgc caatattcgc
      601 aagacgcgag cgcgggcaac ggcggagatt tgggctggtt tgccgacggc gtgatggttc
      661 ccgcctttga agaagccgtc cacgcgctca aacccggaca ggtcggcgcg cccgtccgca
      721 cccaattcgg ctggcatatc atcaaattga acgaagtgcg cgatgccggc acacctcagg
      781 aacgtatccg caattccgtg cggcaataca tcttccaaca aaaagccgaa caggcaaccg
      841 tcaacctgtt gcgtgacctg cattccggcg cgtatgtcga catccgctaa ggcggtttga
      901 agcaaaaagc cataccgatc ggcaaaaatc cgggcggtat ggctttttgg atttcgagtt
      961 acttttacac cgtcattcat cattcccgcg aaagcgggaa tctagaaacg aaaagtaaca
     1021 ggaatttatc gggaatggct ggagtttaaa ggactggatt cccgccgtcg cgggaatgac
     1081 gggattttgg gttgtggtaa tttatcggaa aaacaaaaaa acctatgccg tcattcccga
     1141 gcaggcggga atccggttat ttaaaactgc agaaatttat ccgaagcaac aacaatcttt
     1201 ccatcgtcat tcccgcgtag gcg
//
LOCUS       DAWHSK010000337         1223 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.337, whole genome shotgun sequence.
ACCESSION   DAWHSK010000337 DAWHSK010000000
VERSION     DAWHSK010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1223
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.337"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            166..528
                     /locus_tag="WIC03_002086"
     CDS             166..528
                     /locus_tag="WIC03_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ3884535.1"
                     /translation="MKAARRQLYYTFANRIAEQSRIICIKKLNLLNLQCIQIKVNKLH
                     QKIVASSLLLHMLKEGAAKNGVHIEEIKANATSQSCYSCGRLNKRIGVAATRTNMVCE
                     SFGRHYDTDENASSNIRK"
BASE COUNT          407 a          249 c          203 g          364 t
ORIGIN      
        1 ttcctaaccg gaaaataatt gtcttttccc ttgcaaataa ttgaaattaa taaattacta
       61 aaacacagcc gaatgtaaaa accaattggt acaaatggcg aagataacat ttggctcaaa
      121 cgtaccgagc ggctacgcat tgaatggtaa aatacctacc gcaaattgaa agctgcaaga
      181 cgacagctct actatacgtt cgccaacaga attgccgaac aaagccggat aatctgtatc
      241 aaaaaattga atctgctcaa cctacagtgc atacagatta aagtcaacaa actccaccag
      301 aagatagttg cctcatccct actgctgcat atgctgaaag aaggtgcagc taaaaatgga
      361 gttcatatcg aagagattaa agccaatgct accagccaat catgttactc ttgtggtcgc
      421 ttgaacaaac ggataggtgt tgctgccaca agaaccaata tggtttgtga gagttttggt
      481 cggcattacg acactgatga aaatgcctcc tccaatattc gaaaataggg agctgaaaag
      541 ctaggcacat agagtagcag ttgaccgtaa aatcataaac gcttcggaat aaaaagatgt
      601 agtgatcgca tccttgcatt cctttttatg ttttgttgct ttagcagttt tacagtttag
      661 caactgtaca ttttggcatt tttaactttt catatacgaa ccaacatttt taaatactgc
      721 aaatattttg tacaaaatat attcattatg cctttttgat ttctcatcat taatatctcg
      781 tttatattaa tattgcaaat aatttaaaca aattttagga aaggaaacta attgcggtaa
      841 catctaaaat ccaaagaact gcttctttct tttttacctt cttgctggtt ttatgcttca
      901 acagttgtac agttttactc catgcaaacc aaacaacaaa aaaagaccga cgaagaagtc
      961 ttctgcttag aatttaaaga atactggagt aggggactcc cgttttccgc gacatcattt
     1021 ccaaatagac acggggtacg ctcagcattt catctgaacc atatccatct ttttccccag
     1081 ttttaacctt gtgaaacatc cagtcctttt tcaaatcttc agacagaaaa gaaatacttt
     1141 cctgtttgat tgacttgaag agaaccatcc taccatcact cttgtaagaa aaggcagtat
     1201 ctaatacctg tcttctagag tcg
//
LOCUS       DAWHSK010000338         1215 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.338, whole genome shotgun sequence.
ACCESSION   DAWHSK010000338 DAWHSK010000000
VERSION     DAWHSK010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.338"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            692..943
                     /locus_tag="WIC03_002087"
     CDS             692..943
                     /locus_tag="WIC03_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin B"
                     /protein_id="HEZ3884536.1"
                     /translation="MYIPLPTVIAIQSNIMAIVSTPHPALSALIILAHYHGIAAKSLG
                     LKAKVVRQPVKRLAMVTLPALVWCDDGNHFKEKWLFSAF"
     gene            916..1215
                     /locus_tag="WIC03_002088"
                     /pseudo
     CDS             916..1215
                     /locus_tag="WIC03_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595-like element ISNme4 family transposase"
BASE COUNT          359 a          266 c          208 g          382 t
ORIGIN      
        1 acaatatgaa gtatataaaa tatatttttt ggcatctaaa aaataaactt ttagcaatca
       61 tatataaata gcttgtagaa taaaaaacga aaattattaa ttatcgtagg tgtgcattta
      121 ttccaacacg gtttcatgca gcttaaaaac caaaaattct ttctcccttt atcctttctt
      181 aaaaatagcg ttttcaaatt ctgcaacgac aggtttcatt caagtaggac tgccgtgatt
      241 gcatcaattg cttcgcggca tggcatccat atcacacaac aggacattta ttctcgggta
      301 agtccacctt catcctcttc cgccagcagg aacagttaca ggctacctaa aatatgctct
      361 gtgctcggat agtaaatatt tcctttctca ctattgtttg tcatttaatc gacagtctac
      421 tctttctcta aaatttcgtc aataatagtt aaatatattt aaatacattt aaaataataa
      481 tatacacctg tttcagtctg tctaaaacac gggaaaatat gttatttgat cacaacggtg
      541 actttgggca gcctgtacct tcaataacgc gtggaaaata gatatatact tatatcgtat
      601 tcgaggctgt cttgtttccc tcaaacaacc attcttccac acttttgctt tacaattttc
      661 gtgtgtatcc ttacaattac ccctattgtt tttgtatatt cctttgccaa ccgttattgc
      721 tatccaatct aatattatgg ctatcgtatc tacaccacac cccgctcttt ccgccctcat
      781 catcctcgcc cattatcacg gcattgccgc caaatctttg ggattgaagg caaaggtagt
      841 acgccagcct gtcaaacgtt tggctatggt gaccttacct gcattggtat ggtgtgatga
      901 cggcaaccat tttaaggaaa agtggttgtt ttcggcattc tgaaacgcaa cggacgggtc
      961 tataccgttg tggtggataa tgccaagtct gaaacgttac tccctgtcat caaaaagaaa
     1021 atcatgccgg acagcattgt ttataccgat agtctgagca gctgcgacaa gttggacgtg
     1081 agcggtttta tccattaccg catcaaccat tccaaggagt gtgcagaccg tcagaaccac
     1141 attaacggca ttgagaactt ttggaatcag gcaaaacgcg tcttgcgaaa atacaacgga
     1201 atcgatcgta aatct
//
LOCUS       DAWHSK010000339         1187 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.339, whole genome shotgun sequence.
ACCESSION   DAWHSK010000339 DAWHSK010000000
VERSION     DAWHSK010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.339"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(142..>1187)
                     /locus_tag="WIC03_002089"
     CDS             complement(142..>1187)
                     /locus_tag="WIC03_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEZ3884537.1"
                     /translation="EPLGIEVVRLFGSLRKKAKDEAKAKLADGSVKIAVGTHALFSDG
                     VAFHNLGLTIVDEQHRFGVAQRLALKNKGREVHQLMMSATPIPRTLAMSFFADLDVSV
                     IDELPPGRTPIKTRLVNNVRRAEVEGFVLNTCRKGRQAYWVCPLIEESETLQLQTAAE
                     TLARLQTALPELNIGLVHGRMKAAEKAEVMARFSSGGLNVLVATTVIEVGVDVPNAAL
                     MVIEHAERMGLAQLHQLRGRVGRGAAESVCVLLFAEPLGELAKARLKVIYEHTDGFEI
                     ARQDLNIRGPGEFLGARQSGVPMLRFAKLEEDLHLLEQAREIAPILIEQNPEIVEAHL
                     ARWLSGREGYLGV"
BASE COUNT          238 a          332 c          347 g          270 t
ORIGIN      
        1 ccgataagtc tctgcggctt cccattgccg gatttccgcc cacttgggaa taacgagatt
       61 taaaatcatg aaaatgtgtc aaaaataatg caaaatcaga ttccgttgca cttttttcca
      121 atatttcaga cggcattttg ctcacacgcc caaataacct tccctgccgg aaagccatct
      181 tgccaaatgc gcttcgacga tttcggggtt ttgctcaatc agtatcgggg cgatttcgcg
      241 cgcttgttcc aagaggtgca ggtcttcttc gagcttggcg aaacgcagca taggcacgcc
      301 gctttggcgc gcgccgagaa attcgccggg gccgcggatg ttgaggtctt ggcgggcgat
      361 ttcaaagccg tcggtgtgtt cgtagatgac tttcagccgc gctttggcga gttcgcccaa
      421 gggttcggca aacaggagga cgcacacgct ttctgccgcg ccgcgcccga cccgaccgcg
      481 taattggtgc agctgcgcca agcccatgcg ctcggcgtgt tcgatgacca tcagggcggc
      541 attgggcaca tctacgccga cttcgatgac ggtggtggcg accaagacat tcagcccccc
      601 cgaagaaaac cgcgccatca cttcggcctt ttcggcggcc ttcatgcgcc cgtgtaccag
      661 tccgatattg agttcgggca atgccgtctg aagccgggcg agggtttcgg cggcggtttg
      721 cagttgcagg gtttcgcttt cttcaatcag cggacaaacc caatacgcct gccgcccttt
      781 gcggcaagta ttgaggacga agccttcgac ttcggcgcgg cggacgttgt tgacgaggcg
      841 cgttttaatc ggcgtgcgcc cgggcggcag ttcgtcgatg acggacacgt ccaaatcggc
      901 gaaaaaactc atcgcaagcg tgcgcgggat gggcgtggcg gacatcatca gctgatggac
      961 ttcgcgccct ttgtttttga gggcgaggcg ttgggcaacg ccgaaacggt gctgttcgtc
     1021 cacaatggtc aagcccaaat tgtgaaacgc cacgccgtct gaaaacaggg cgtgcgtgcc
     1081 gacggcgatt ttgacgctgc cgtcggcgag tttggctttg gcttcgtctt tggctttttt
     1141 acgcaaactg ccaaaaaggc ggacaacttc aatgcccaaa ggttcga
//
LOCUS       DAWHSK010000340         1143 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.340, whole genome shotgun sequence.
ACCESSION   DAWHSK010000340 DAWHSK010000000
VERSION     DAWHSK010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.340"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            184..1083
                     /locus_tag="WIC03_002090"
     CDS             184..1083
                     /locus_tag="WIC03_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ3884538.1"
                     /translation="MGKGGDIVVGNGKKYAAPLLVLGCVVFGLGSLIVRSVPVGSYAI
                     AFWRLLISVFVFWFLARFFGQKFPKNRKTVRYALTAGVFLAFDLALWHESIHAVGPGI
                     STLLNSLQIFFLSAIGVFFFGERLSGLKKAGLISAVAGVAMIAGAEFGYNGNAVWGFA
                     SGLVSGLMLALSMVFVRKTHEIEPVALFPSMMILSLGGAVSLVVPALLMDGGALYPTT
                     WKDAGLVLVYGVVMQCFAWAMVAYAIPLLSLSLTGLLLLSEPVAALFIDYFGLGKPIE
                     GVQWAGVALTLSAIYLGSLKQQS"
BASE COUNT          196 a          259 c          357 g          331 t
ORIGIN      
        1 ccgtactggt ttttgttaat ccactatatt cgggttttat ccgacaggac ggttttttgc
       61 cccaacggaa aatagtcggt ctgcccgtaa aatcagccgt ttgtccgggt gcagccgggg
      121 ctttgggctt cagacggcat attttcggaa tggcggcatt cttgccggcg gcgcggcagc
      181 cgtatgggga agggagggga tattgtggtc ggtaacggca aaaaatatgc cgcaccattg
      241 ctggtgctgg gttgcgtggt gttcggtctg ggcagtctga ttgtcagatc cgtccccgtc
      301 ggttcgtatg caatcgcatt ttggcggttg ctgatttcgg tgttcgtatt ttggttttta
      361 gcacggtttt tcgggcaaaa attcccaaaa aacaggaaaa ccgtccgata tgccctgacg
      421 gcgggcgtgt ttctcgcttt cgatttggcg ttgtggcacg aaagcataca cgcggtcggg
      481 ccgggtattt ccaccctgct caacagcctg caaatctttt tcttgtcggc aatcggtgtt
      541 ttctttttcg gcgagcgttt gagcgggctg aaaaaggcag gcttaatatc ggcagttgcc
      601 ggcgtggcga tgattgccgg tgcggaattc ggctacaacg gtaatgcggt ttggggattc
      661 gccagcggtt tggtatcggg actgatgctc gccctgtcga tggtgtttgt ccgcaaaacc
      721 catgaaatcg agccggtggc gcttttccct tcaatgatga ttttgagttt gggcggcgcg
      781 gtatcgctgg ttgttccggc attgctgatg gatggcggcg cgctttatcc gacgacttgg
      841 aaagatgcgg gtttggtgct tgtgtacggc gtggtaatgc agtgttttgc gtgggcgatg
      901 gttgcctatg ccattccgct gctttcgctg tcgctgacgg ggctgctgct tttgtccgaa
      961 ccggtcgctg ccttgttcat cgattatttc ggattgggca aaccgattga aggcgtgcag
     1021 tgggcagggg tggcgctgac gctttcggca atttacctcg gttcgctgaa acagcagtct
     1081 taatattgat tccatcaagg cagtattggc agttttccgt gcaaaacctg cgttaccgga
     1141 ctg
//
LOCUS       DAWHSK010000341         1122 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.341, whole genome shotgun sequence.
ACCESSION   DAWHSK010000341 DAWHSK010000000
VERSION     DAWHSK010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.341"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..789
                     /gene="mafB"
                     /locus_tag="WIC03_002091"
     CDS             <1..789
                     /gene="mafB"
                     /locus_tag="WIC03_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HEZ3884539.1"
                     /translation="NPFISAGEALGIGDILYGTRYAIDKAAMRNIAPLPAEGKFAAIG
                     GLGSVAGFEKNTREAVDRWIQENPNAAETVEALVNVLPFAKVKNLTKAAKPGKAAVSG
                     DFSAAYNTRTTRKVTTETEGLNRIRQNQKNSNIHEKNYGRDNPNHINVLSGNSIQHIL
                     YGDEAGGGHLFPGKPGKTTFPQHWSASKITHEISDIVTSPKTQWYAQTGTGGKYIAKG
                     RPARWVSYETRDGIRIRTVYEPATGKVVTAFPDRTSNPKYNPVK"
     gene            804..1034
                     /locus_tag="WIC03_002092"
     CDS             804..1034
                     /locus_tag="WIC03_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
                     /protein_id="HEZ3884540.1"
                     /translation="MKTLDERIKNLGKSLEDRIDANLIDAALEYITFSERLLAFETLC
                     DYIEDFNIQLTEKESQEISFINKEFGIESTSD"
BASE COUNT          394 a          228 c          239 g          261 t
ORIGIN      
        1 aaccccttta tcagcgcggg cgaagccttg ggcataggcg acatactgta cggaacgcgc
       61 tatgccatag acaaagccgc aatgcgcaac atcgccccct tacccgccga gggcaaattc
      121 gccgccatcg gcggcttggg cagcgtggcg ggctttgaaa agaatacgcg cgaagccgtt
      181 gaccggtgga tacaggaaaa ccccaatgcc gccgaaaccg tcgaagccct ggtcaacgtc
      241 ctgccgtttg ccaaagtcaa aaacctgaca aaggcggcaa aaccggggaa ggctgcggtt
      301 agcggggact tttctgctgc atacaataca agaacaacta gaaaagttac tacagaaaca
      361 gaggggttaa atagaatcag acagaaccag aaaaatagta atatacatga gaaaaattat
      421 ggaagagata atcctaatca tattaatgtt ttatctggaa attctataca acatatactg
      481 tatggagatg aagcaggagg tgggcatctt tttcctggca aacctggtaa gacaacattc
      541 ccccaacatt ggtcagccag taaaataact catgaaatta gtgatatcgt tacatcccca
      601 aaaacgcaat ggtatgcaca gactggaaca ggcggcaaat atattgctaa aggaagacca
      661 gccaggtggg tatcatatga aacgagagat ggaattcgta tcagaacagt ttatgaacct
      721 gcaacaggaa aagtggtaac tgcattcccc gatagaacct ctaatcccaa atataaccct
      781 gtaaaataat gagtatttga aagatgaaaa cattagacga acgaataaaa aatttaggaa
      841 aatctcttga agatagaatt gacgctaatc taattgatgc cgcactagag tacattactt
      901 tttcagaacg tttgctagct tttgaaacgc tatgtgatta tatagaagat ttcaatatcc
      961 agcttacgga aaaagaatcc caagaaatct cttttattaa taaggaattt ggaattgaaa
     1021 gtacatcaga ttaataaata ttaggtcggt ttaaaaaccc aacaatcaaa aagtcgtctg
     1081 aaactgtgtt taatttacag acgacctttc cttcatttga ag
//
LOCUS       DAWHSK010000342         1122 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.342, whole genome shotgun sequence.
ACCESSION   DAWHSK010000342 DAWHSK010000000
VERSION     DAWHSK010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.342"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(230..1084)
                     /locus_tag="WIC03_002093"
     CDS             complement(230..1084)
                     /locus_tag="WIC03_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEZ3884541.1"
                     /translation="MFYQILALIIWSSSFIAAKYVYGGIDPALMVGVRLLIAALPALP
                     ACRRHVGKIPREEWKPLLIVSFVNYVLTLLLQFVGLKYTSAASASVIVGLEPLLMVFV
                     GHFFFNDKARAYHWICGAAAFAGVALLMAGGAEEGGEVGWFGCLLVLLAGAGFCAAMR
                     PTQRLIARIGAPAFTSVSIAAASLMCLPFSLALAQSYTVDWNVGMVLSLLYLGLGCGW
                     YAYWLWNKGMSRVPANVSGLLISLEPVVGVLLAVLILGEHLSPVSALGVFVVIAATLV
                     AGRLSHQK"
BASE COUNT          311 a          337 c          294 g          180 t
ORIGIN      
        1 cccgtcattc ccgcgcaggc gggaatccgg acatccaacg ctgcagcaat ttgtcggaaa
       61 taaccgaaac tcaaaaaacc ggatgcccgc ctgcgcggga atgacgatgc aaagatggtc
      121 gttatttcgg ataaatatcc gtcttttcgt tccggatacc cgctttcgca ggaatggaga
      181 tggaaagatt tcagcctatt cggcaatcat caaataccgc ttcccaactt tatttttgat
      241 gcgacagccg gccggcaacc aaggtggcgg cgatgacgac aaacacgccc aaggcggaca
      301 cgggcgacag gtgttcgccc aaaatcaaaa ccgccagcag cacgccgacg acgggttcga
      361 gcgaaatcaa cagtcccgaa acgttggcag gaacacggct catccccttg ttccacagcc
      421 aataggcgta ccagccgcac cccaagccca aatacagcag cgacaatacc atcccgacgt
      481 tccagtccac ggtataactt tgcgccaaag caagcgaaaa cggcaggcac atcaacgatg
      541 cggcggcaat ggaaacagat gtgaatgccg gtgcgccgat gcgtgcaatc agcctttgcg
      601 tcggacgcat agcggcacaa aagcccgcgc ccgccaacaa caccagcagg cagccgaacc
      661 agccgacttc gccgccctct tccgcaccgc ccgccatcag cagcgcgaca ccggcaaatg
      721 ccgccgcgcc gcatatccag tggtaggcac gcgctttgtc gttgaagaaa aagtgtccga
      781 caaacaccat cagcagcggc tcgagtccga caatgaccga tgcgctggcg gcggaagtgt
      841 atttcaaccc gacaaactga agcagcaggg tcagcacata gttgacgaac gacacaatca
      901 gcaacggctt ccattcctca cgcggaatct tgccgacatg acggcggcag gcgggcagtg
      961 caggcagcgc agcaatcagc aggcgcacgc cgaccatcaa tgcgggatcg atgccgccat
     1021 agacatattt ggcggcaata aacgagctgc tccagataat cagggcaagg atttggtaaa
     1081 acatagtgag tgataaagtc agatggtcat acaaaagtat ag
//
LOCUS       DAWHSK010000343         1087 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.343, whole genome shotgun sequence.
ACCESSION   DAWHSK010000343 DAWHSK010000000
VERSION     DAWHSK010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.343"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..919)
                     /locus_tag="WIC03_002094"
     CDS             complement(<1..919)
                     /locus_tag="WIC03_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ3884542.1"
                     /translation="MENQRPLLGFALALLAAMTWGTLPIAVRQVLKFVDAPTLVWVRF
                     TVAAAVLFVLLALGGRLPKWRDFSWCSFRLLLLGVAGISANFVLIAQGLHYISPTTTQ
                     VLWQISPFTMIVVGVLVFKDRMTAAQKIGLVLLLAGLLMFFNDKFGELSGLGAYAKGV
                     LLCAAGSMAWVCYAVAQKLLSAQFGPQQILLLIYAASAAVFLPFAKPAHIGSLDGTLA
                     WVCFAYCCLNTLIGYGSFGEALKHWEASKVSAVTTLLPVFTVIFSLLGHYVMPDTFAA
                     PDMNGLGYAGALVVVGGAVTAAVGDRLFKR"
BASE COUNT          302 a          354 c          237 g          194 t
ORIGIN      
        1 ggcgtttgaa cagcctgtcc cccaccgccg ccgtaaccgc acccccgacc acgaccagtg
       61 cgccggcata acccaaaccg ttcatatccg gcgcggcaaa agtatcaggc atcacataat
      121 gcccgagcaa agaaaatatt acggtaaaca cggggagcaa ggttgttacc gcgctgactt
      181 tggaagcctc ccaatgtttc aacgcctcgc cgaacgagcc gtaaccgatt aacgtattca
      241 agcagcaata cgcaaaacaa acccacgcca acgtaccgtc caaacttccg atgtgtgccg
      301 gtttggcaaa cggcaggaac acggcggcac ttgccgcata aatcaacagc agaatctgtt
      361 gcggcccgaa ttgcgccgac agcagctttt gcgccacggc ataacacacc catgccatac
      421 tgcctgccgc acacagcaac acgcccttcg catacgcgcc caaacccgac aactcgccga
      481 atttatcgtt aaaaaacata agcaaaccgg caagcagcaa aaccaagccg attttctgag
      541 cggcagtcat ccggtcttta aacaccaaca caccgacaac aatcatcgta aacggcgaaa
      601 tctgccacaa aacctgcgtc gtggtcggcg aaatataatg cagcccttgg gcaatcagca
      661 caaagtttgc cgaaatgccc gccacgccga gcagcagcag cctgaatgag caccaagaaa
      721 aatcccgcca cttcggcagc cgcccgccca gtgccagcaa aacaaacaat accgccgccg
      781 ccacggtaaa acgcacccac accagcgtcg gcgcatcgac aaacttcaat acctgccgca
      841 cggcaatcgg cagcgttccc cacgtcatcg ccgccaaaag tgccaacgcg aagcctagga
      901 gcggcctttg gttttccatc ctgattttcc tatttttaaa caaccgtatt gccggacgat
      961 gccggtttgc cgcatcgggc aatgatggtt caagcgtttg gcgtttgatt ccaacccttt
     1021 gatttcaaac aaaccggctg aagctcggct attgcttcgc gctatttgaa aacaccgcct
     1081 gaatttt
//
LOCUS       DAWHSK010000344         1085 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.344, whole genome shotgun sequence.
ACCESSION   DAWHSK010000344 DAWHSK010000000
VERSION     DAWHSK010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.344"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            559..>1085
                     /locus_tag="WIC03_002095"
     CDS             559..>1085
                     /locus_tag="WIC03_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FrpC operon protein"
                     /protein_id="HEZ3884543.1"
                     /translation="MRPYATTIYQLFILFIGSVFTMTSCEPVNEKTDQKAVSAQQAKE
                     QTSFNNPEPMTGFEHTVTFDFQGTKMVIPYGYLARYTQDNATKWLSDTPGQDAYSINL
                     IEISVYYKKTDQGWVLEPYNQQNKAHFIQFLRDGLDSVDDIVIRKDACSLSTTMGERL
                     LTYGVKKMPSAYPEY"
BASE COUNT          361 a          187 c          207 g          330 t
ORIGIN      
        1 ccaaaaacag caaaacaaat tgtaaggata cgtataggct ttgtaaaggt aaattgtgaa
       61 aaaagcagtt ttttaaacga atgaaacggc ttcgggctga aatatatgct gatgccctgt
      121 tcttcccgta tttctcgtgt gttgtcaaag tgcaggctgc tttgaaatcg gtattgccat
      181 ctatgaacca ccactttgct ttatttcagc gggcttgaga tgtgtataag aatattgttt
      241 tgaataaatt taaagaaaat gataatcgtt attgacgatt tttaaaggaa agcgtagagt
      301 gccaattcta tgaagcaata cggtaagtaa caatgaaaat atctactgct tgggtataga
      361 gcatatttca caacccgtaa ctattcttgc ggaaacagag aaaaaagttt ctcttctatc
      421 ttggataaat atatttaccc tcagtttagt taagtattgg aatttatacc taagtagtaa
      481 aagttagtaa attattttta actaaagagt tagtatctac cataatatat tctttaacta
      541 atttctaagc ttgaaattat gagaccatat gctactacca tttatcaact ttttattttg
      601 tttattggga gtgtttttac tatgacctca tgtgaacctg tgaatgaaaa gacagatcaa
      661 aaagcagtaa gtgcgcaaca ggctaaagaa caaaccagtt tcaacaatcc cgagccaatg
      721 acaggatttg aacatacggt tacatttgat tttcagggca ccaaaatggt tatcccctat
      781 ggctatcttg cacggtatac gcaagacaat gccacaaaat ggctttctga cacgcccggg
      841 caggatgctt actccattaa tttgatagag attagcgtct attacaaaaa aaccgaccaa
      901 ggctgggttc ttgagccata caaccagcaa aacaaagcac actttatcca atttctacgc
      961 gatggtttgg atagcgtgga cgatattgtt atccgaaaag atgcgtgtag tttaagtacg
     1021 actatgggag aaagattgct tacttacggg gttaaaaaaa tgccatctgc ctatcctgaa
     1081 tacga
//
LOCUS       DAWHSK010000345         1084 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.345, whole genome shotgun sequence.
ACCESSION   DAWHSK010000345 DAWHSK010000000
VERSION     DAWHSK010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.345"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(39..371)
                     /gene="trxA"
                     /locus_tag="WIC03_002096"
     CDS             complement(39..371)
                     /gene="trxA"
                     /locus_tag="WIC03_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HEZ3884544.1"
                     /translation="MSSELIIHTSDAAFEKDVLNSDTPVLLDFWAPWCGPCKMIAPIL
                     DDIAAEFEGRLKVVKINIDDNEATPSRFGVRGIPTLMVFKNGEVVATKVGALAKGQLT
                     AFVEASIA"
     gene            669..>1084
                     /locus_tag="WIC03_002097"
     CDS             669..>1084
                     /locus_tag="WIC03_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEZ3884545.1"
                     /translation="MTDFSVWETAPFGATVDYILQRYHNVHRAQFEELVPLAQKVVQV
                     HADTFPAEIARLLADMQNELLMHMMKEERMLFPMINQGVGRGAAMPISVMMHEHEEHD
                     RAIARLKELTDNFQPPEGACGSWTRLYALAKEMVED"
BASE COUNT          272 a          254 c          287 g          271 t
ORIGIN      
        1 cgccggaatc ttccggcggc tttttcgatt gcgctttatc aggcgatgga ggcttcgaca
       61 aaggcggtca gctgaccttt tgccaatgcg ccgactttgg tagccacgac ttcgccgttt
      121 ttgaacacca tcagggtcgg aatgccgcgc acgccgaaac gggacggggt ggcttcgttg
      181 tcgtcgatgt tgattttgac cactttcaga cggccttcaa attcggcggc gatgtcgtcc
      241 aaaatcgggg caatcatttt gcaggggccg caccacggag cccaaaagtc cagcaggacg
      301 ggggtatctg aatttaaaac gtctttttca aaagcggcat cgctggtgtg tataatcaat
      361 tcgctgctca ttatttttcc ttttcggttg ggattggcac gatagcgtgc aggatagggc
      421 ttgggcataa aaatttcaag tgggcgggcg ggataatagt ttttctgtac cgccccaatc
      481 gcctttgcaa attctaaaaa acggttcgga cagtatttta tttttgaaaa ttacttttat
      541 tatcaaagtg taaggaatgt ttttgaagct gtacagaaac gcgcgggcgg tgtatgctcc
      601 gtttaaatag attcttaaag tatgaatgtt caaaaccaaa cccgaaaccc tataaaagga
      661 acactgcaat gactgatttt tccgtttggg aaacagcccc ctttggcgca accgtcgatt
      721 atatcctgca acgctaccac aatgtccacc gcgcacaatt tgaagaattg gtgccgctgg
      781 cgcagaaagt ggtccaagtc catgccgata cctttccggc ggaaattgcc aggctgcttg
      841 ccgatatgca gaacgaactg ctgatgcata tgatgaaaga ggaaaggatg ctgtttccga
      901 tgattaatca gggtgtcgga cgcggtgcgg caatgcccat cagcgtgatg atgcacgaac
      961 acgaagaaca cgaccgcgcc atcgcacggc tcaaagagct gaccgacaat ttccaaccgc
     1021 cagaaggggc ttgcggaagc tggacgcgcc tttacgcttt ggccaaagaa atggtggagg
     1081 attt
//
LOCUS       DAWHSK010000346         1082 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.346, whole genome shotgun sequence.
ACCESSION   DAWHSK010000346 DAWHSK010000000
VERSION     DAWHSK010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.346"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            284..721
                     /locus_tag="WIC03_002098"
     CDS             284..721
                     /locus_tag="WIC03_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapD"
                     /protein_id="HEZ3884546.1"
                     /translation="MSRYLITFDMDTNCLKDNYHGNNYTNAYSDIKTILARHGFENIQ
                     GSVYLGREGISEAHGTIAIQELTARFDWFYSCISNIKFYRLESDLNAQFIADGVYQAK
                     QAFLQRVEQLRISLTEAGLSDEQINQVLEKQKFELESPNLKLN"
BASE COUNT          338 a          248 c          219 g          277 t
ORIGIN      
        1 gccaccaaaa cccttgctag ctaagggtta aacagctcac ttgaaatcta cttaagtcta
       61 atctaaacta tccaatatgg atagattttt aaacataggg caagcagcaa aattattgta
      121 gctgaaagca caatcactcg ctggtggtct caaacacgtg ccgactacct cgccgaaaac
      181 actatcagcc gcgataaacc gtgggaaaag ctcgttatca gccgccgcac ttggtactat
      241 cgcgggaaac cgatgctgtc tgaaacgcaa caggagaaaa ataatgagcc gttacctgat
      301 tacctttgat atggatacca actgcctgaa agacaattac cacggaaata actataccaa
      361 tgcctactcc gatattaaaa ccatcttggc tagacatgga tttgagaaca ttcagggcag
      421 tgtttatcta ggccgtgaag gcatcagtga agcacacgga acaatagcca ttcaggaact
      481 gaccgctcgg tttgattggt tttactcctg tatttcaaac attaagtttt accgccttga
      541 aagtgatttg aacgcacaat ttatcgctga tggtgtgtat caagccaaac aggctttcct
      601 tcaacgtgtt gaacaacttc gtatatccct aacagaagct ggattgtctg atgagcaaat
      661 caatcaggtt ctggaaaaac agaaatttga attggaaagt cctaacctga aattaaatta
      721 acctccttta ctcaccaaca tccgccgcag ctctgtcagt ttttggcgcg ctgcggcgat
      781 ttctgtgcgt tttagagctt cgggtagggt gtgaaacaac tcactcgaaa tttacttaag
      841 tctaatctaa actatccaag cagtaattag tacaaaaaag gcaaacttat tttaggagct
      901 taaaattgca gccgcgataa accgtgggag agtctcggca tttcccgcgc cacttggtac
      961 aaacgtggca aaccgatgcc gtctgaaacc gtacaacagg agacaaaata atgcttgctc
     1021 aaatcgaact cacaccctgc cagctaatgg tttacgtaac ttaaggttac tggatttacg
     1081 ca
//
LOCUS       DAWHSK010000347         1065 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.347, whole genome shotgun sequence.
ACCESSION   DAWHSK010000347 DAWHSK010000000
VERSION     DAWHSK010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.347"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..486
                     /locus_tag="WIC03_002099"
     CDS             <1..486
                     /locus_tag="WIC03_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884547.1"
                     /translation="KQAAPKETINNLANLAKAEQQILFRIAQRDTQLDAWKTGFNNRV
                     RKGAGLLDASNIPITINGKTIKPVQAISLKGAPVYSGVSEQEIFALYRQMTGQNPNFR
                     VLPDGRLANGIISTGEWAGTKIALRNFSKTENSTQARWTLDLQNPPSFIKGTKLELKF
                     Q"
     gene            505..879
                     /locus_tag="WIC03_002100"
     CDS             505..879
                     /locus_tag="WIC03_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF596 domain-containing protein"
                     /protein_id="HEZ3884548.1"
                     /translation="MDEKQKIKILDFQIDLSSIFNSYKNQMGINIQDKNLKNNFCSFM
                     EELLNDGSIRLHDYTDGIGIPLTGTSKEQVQKLKDIWPTLEDAQAIWPEDPWYYLEWL
                     WWDIACPIDLADLPNIDIYEQA"
BASE COUNT          363 a          193 c          215 g          294 t
ORIGIN      
        1 aagcaagctg ctcctaaaga aacaataaac aatttggcaa atttagccaa agcagaacag
       61 cagattttat tccgtattgc ccaacgcgat acgcaactgg atgcatggaa gacgggattt
      121 aacaatagag taaggaaagg agcaggcttg cttgatgcaa gtaatattcc gataaccatt
      181 aacggaaaaa ccatcaaacc tgtacaagcc ataagcttaa agggagcacc cgtttacagc
      241 ggcgtaagcg aacaggagat ttttgcgctt tatcggcaga tgactggcca gaatccgaat
      301 tttagagttt tgcctgacgg aagattagca aatggcatta tcagtactgg agaatgggca
      361 ggaacaaaaa ttgcattaag aaatttttca aaaacagaga attcaactca agcacgatgg
      421 acattagatt tgcagaatcc tccatcattt attaaaggta ctaaattgga gcttaaattc
      481 caataattta caaaggattt taccgtggat gagaaacaaa aaattaagat tcttgatttt
      541 caaatcgatt tatcctcaat ttttaactct tataaaaatc aaatgggtat taatattcaa
      601 gataaaaact taaaaaacaa tttctgttct tttatggaag aactgttaaa tgacggttca
      661 atccgtttac atgattatac cgacggtatc ggaattcctc taactggaac ttcaaaagaa
      721 caagtgcaga aattgaaaga catatggcct actttggaag atgcccaagc aatatggcct
      781 gaagaccctt ggtattactt agaatggctt tggtgggata ttgcgtgtcc aatagatttg
      841 gccgatttgc cgaatattga tatttatgag caagcgtagg tatggttagc cgcctttagc
      901 ggcgtaaccg tacgcatatc agcaaacttt ataaaataac aaggccgtct gaaatctgtt
      961 tttcaacttt ttcagacggc cttgcaactt ggcatttcat tcgtacggtt acgccgctaa
     1021 aggcggctaa ccgtacctac gagctctgat aaaaatgatt tatgg
//
LOCUS       DAWHSK010000348         1065 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.348, whole genome shotgun sequence.
ACCESSION   DAWHSK010000348 DAWHSK010000000
VERSION     DAWHSK010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.348"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(27..350)
                     /locus_tag="WIC03_002101"
     CDS             complement(27..350)
                     /locus_tag="WIC03_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEZ3884549.1"
                     /translation="MDMVVRGGIYLVSLDPTVGSEIKKTRPCVVVSPPEIHNYLKTVL
                     IIPMTSGSRPAPFRVNVRFQDKDGLLLPEQIRAVDKAGLVKHLGNLDNSTAEKLFAVL
                     QEMFA"
     gene            complement(338..577)
                     /locus_tag="WIC03_002102"
     CDS             complement(338..577)
                     /locus_tag="WIC03_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="growth regulator"
                     /protein_id="HEZ3884550.1"
                     /translation="MILNIRKMGNSQGVILPKSLLGQIGAVDSLAVTVEKGNIILSCP
                     TVRRGWAEAAAMLVETEQEHFFSEIENEADKEWIW"
     gene            complement(700..>1065)
                     /locus_tag="WIC03_002103"
     CDS             complement(700..>1065)
                     /locus_tag="WIC03_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEZ3884551.1"
                     /translation="QMRQAAALLIGEQDFSSFRAAECQAKSPVKTIYRADLTQSAGLV
                     RLDLHGNAFLHHMVRNIMGALVYVGSGRLSVEGFAALIQERSRLKAPPTFMPDGLYLT
                     GVDYPEAYGIIRPQIPEWL"
BASE COUNT          254 a          253 c          275 g          283 t
ORIGIN      
        1 gaacacaatc cattcagact attcaatcag gcaaacatct cctgcaatac tgcaaacagt
       61 ttttcagccg tactgttgtc taaatttcca agatgtttga ccaatccggc tttatccaca
      121 gccctaatct gttcgggcaa aagcaaaccg tctttatcct gaaagcggac attgacgcgg
      181 aacggggcag gacggcttcc gctcgtcatg ggaatgatca gcacagtctt gagatagttg
      241 tgtatttcag gaggagatac tacgacacaa ggacgtgtct ttttgatttc gcttcctacg
      301 gtcgggtcta aggagaccag atagattccg ccgcgtacta ccatatccat tctttatccg
      361 cttcgttttc aatttcggaa aaaaaatgct cctgctcggt ttcgacaagc attgcggcag
      421 cttctgccca tcccctgcga acggtaggac agcttaaaat gatattgccc ttttcaactg
      481 taacagccaa gctgtctact gcccctattt gacccaataa tgatttgggc agaatcacgc
      541 cttgcgagtt tcccattttg cgtatgttga gaatcatata cgtaccctat ttaccagttt
      601 atgtaataac aatgttatta ccttgatgag gtagtgtcaa tattgaaaaa gattgcctga
      661 cagtttgtcc gatttcaaaa tctccgcgac aagaatgttt taaagccatt cggggatttg
      721 ggggcggatg atgccgtatg cctcgggata gtcgacgccg gtcaggtaaa gtccgtcggg
      781 catgaaggtc ggcggggctt tgaggcggct gcgttcttga atcagtgcgg cgaagccttc
      841 gacgctgagt ctgccgctgc cgacatagac gagcgcaccc atgatgttgc gtaccatatg
      901 gtgcaaaaag gcgttgccgt gcaaatcgag gcggacgagt cctgcgcttt gggtaaggtc
      961 ggcgcggtag atggttttga cgggggattt tgcctggcat tcggcggcgc ggaagctgga
     1021 gaagtcttgt tcgccgatca ataaggcggc agcctgccgc atctg
//
LOCUS       DAWHSK010000349         1062 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.349, whole genome shotgun sequence.
ACCESSION   DAWHSK010000349 DAWHSK010000000
VERSION     DAWHSK010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.349"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(414..>1062)
                     /gene="xth"
                     /locus_tag="WIC03_002104"
     CDS             complement(414..>1062)
                     /gene="xth"
                     /locus_tag="WIC03_002104"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEZ3884552.1"
                     /translation="FPAAALQMMGWHCVWSGQKTYNGVAIVSRNAPEDVHIGLPALPD
                     DPQRRVIAATVGGVRVINVYCVNGEALDSPKFKYKEQWFAALTEFVRDEMTRYGKLVL
                     LGDFNIAPADADCYDPEKWHEKIHCSSVERQWFQNLLDLGLTDSLRQVHPEGAFYTWF
                     DYRGAMFQRKLGLRIDHILVSPAMAAALKDVRVDLETRALERPSDHAPVTAEFDW"
BASE COUNT          271 a          276 c          247 g          268 t
ORIGIN      
        1 ttgcctttgg gagaatcaat tctgacagca cataacatag gttcatcttt acagtcgtta
       61 tattgggttt catcaaactc aactgcacag cacaataaag acaataggaa aaaaaggaga
      121 ttaatttatt actatacata aggcagcctg attattcatt ctaaaataaa tccggttttc
      181 aatatttgat tttattccgg attatataca caataaattt taccgctcca aaatctgata
      241 ttaaccctat ggatttcata aattcttcag aaaaataggc tgtgcttgat caatccgccg
      301 actccggctg tttgtcaggg aaaggcaatt atttttcggt caggcagaaa gttatcctat
      361 cgaatcactt gaagatatga atgcttgtca acgccatatc aaaacacggt cttttaccaa
      421 tcgaattctg ccgtcaccgg cgcgtggtcg ctcggacgct ccagcgcgcg cgtctccaaa
      481 tcgacacgga catccttcaa cgccgccgcc atcgcaggcg acaccaaaat atggtcgata
      541 cgcaggccca gtttgcgttg gaacatcgcg ccgcgatagt cgaaccaggt atagaacgcg
      601 ccttcgggat ggacttggcg caggctgtcg gtcagtccca aatccagcag gttttgaaac
      661 cactgccgtt cgacggacga acagtggatt ttttcgtgcc atttttcagg gtcgtaacag
      721 tccgcatcgg caggcgcgat attgaaatca cccagcagca ccagtttgcc gtagcgggtc
      781 atttcatcgc ggacaaactc cgtcagtgcg gcaaaccatt gttctttata tttgaatttc
      841 gggctgtcga gggcttcgcc gttgacgcaa tagacattga tgacgcgcac accgccgacg
      901 gttgccgcaa tcacgcgccg ttgcggatcg tccggcagcg cgggcagccc gatatgcacg
      961 tcttccggcg cgttgcggct gacgattgcc acgccgttgt aggttttctg cccgctccaa
     1021 acacagtgcc agcccatcat ttgcaaagcg gcggccggaa at
//
LOCUS       DAWHSK010000350         1039 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.350, whole genome shotgun sequence.
ACCESSION   DAWHSK010000350 DAWHSK010000000
VERSION     DAWHSK010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.350"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(91..642)
                     /locus_tag="WIC03_002105"
     CDS             complement(91..642)
                     /locus_tag="WIC03_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884553.1"
                     /translation="MKVLNGWSDRKMWRVLSALPIGVVFFDLIYGFVLNVLQGLDLQR
                     AVPDSEGVLAVTPDIAFNSLQIVANGGMAAVVCFGLAVVFLLNRSVRRRQVLEIGVFR
                     MLGLVAVLAFSAPSVWEWANALPLLLKGADVVNTGNARYVLTALCMPFPAVSCVIGLV
                     GRFRLQTASGRAAKAGGAVKAGG"
     gene            complement(696..1039)
                     /locus_tag="WIC03_002106"
                     /pseudo
     CDS             complement(696..1039)
                     /locus_tag="WIC03_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557955.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          305 a          341 c          193 g          200 t
ORIGIN      
        1 caaagaaacc gccattagca ctacaccccc ccaagctgcg gctgccaaac acatcggctg
       61 cttctcgacg cacccgctga aaaatgcgcc ctatccgccc gccttgaccg caccccctgc
      121 ctttgccgcc ctgcccgatg ccgtctgaag cctgaaccgc cccaccagcc cgatgacgca
      181 cgacaccgcc ggaaagggca tacacaaagc cgtcagcaca taacgcgcat tccccgtatt
      241 gaccacgtcc gcgcccttca gcagcagcgg cagcgcgttc gcccactccc acaccgacgg
      301 cgcgctgaac gccaataccg ccaccagccc caacatccgg aacaccccga tttccaacac
      361 ctgccgccgc cgcaccgaac ggttgagcaa aaacacaacc gccaacccga aacagaccac
      421 cgccgccata ccgccgttgg cgacaatctg caaactgttg aatgcaatat cgggcgtaac
      481 cgccaacacg ccttccgaat ccggcacggc acgctgcaaa tccaaaccct gcaacacatt
      541 caacacaaaa ccgtagatca aatcaaaaaa caccacgcct atcggcaaag cactcaaaac
      601 ccgccacatc ttcctgtccg accaaccgtt caaaaccttc attttacccg accacgcaag
      661 ccgccgaaca aaaaataagg ggctgtccta gataactagg ataaactcga ttttactaat
      721 tgttttaaaa tggaacaaga acttttatct cactgttgtt aaaacgccat tcgcactcct
      781 ttaaatacag ctcaaaatgc gctttgggaa tgccgttaaa cttgcgtaaa tgacgttttg
      841 cctggttcca aaagttctca attccattaa tatggttttg tcgttcagca aaatgtgtgc
      901 tgtgattgat acgaaaacga agtttcagcg aagctaaaat ggctaaattc gcgcacatct
      961 aatacatcat agctacgata acaatccgta taaacaatgc tgtcaggttt cacttgttca
     1021 cggataatag gaaataaag
//
LOCUS       DAWHSK010000351         1035 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.351, whole genome shotgun sequence.
ACCESSION   DAWHSK010000351 DAWHSK010000000
VERSION     DAWHSK010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.351"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(299..685)
                     /locus_tag="WIC03_002107"
     CDS             complement(299..685)
                     /locus_tag="WIC03_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEZ3884554.1"
                     /translation="MRKILLDTHALLWWLLDDKELGISARKLIENPRNAIFVSAASIW
                     EISIKQNKGLLKLPEEFFDVLQEEDFEMLPIGLFHAKQAGSLPEIHKDPFDRMLIAQT
                     QAEGFELMTVDEYIPQYGIRVVNASS"
     gene            complement(682..930)
                     /locus_tag="WIC03_002108"
     CDS             complement(682..930)
                     /locus_tag="WIC03_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEZ3884555.1"
                     /translation="MFQANIHQAKTNLSQLIQRAEAGEIVIIAKAGKPCVQLIGIEKP
                     ARNAGRLKKFSHMENTDISRILEDDNETAALFFEESAL"
BASE COUNT          283 a          247 c          182 g          323 t
ORIGIN      
        1 cagccatata tattaagtac ctgctcttta tgcgcttttt taggaaagga agatcaaacg
       61 gttcaatagc gaagtcttgc ccaaatttga tggacaggat atagaataca tgcgtgcata
      121 ccacaccctg ccaaagttgg gagatgcact taaggttgca cttgtggtcg ccaactatgc
      181 ctatacacgc ggatttcccg cgtttatagg ccgcgacgga agtctggctt ttaagcgaag
      241 acaataaccg aaaagaccgc ctcggcggtc tttcttgtgt acacagtttt tgtatttgtc
      301 agcttgatgc gttgacaact ctaattccat actgcggaat atattcatcg acagtcatca
      361 gttcaaagcc ttccgcttgg gtttgtgcaa tcaacatcct atcgaaaggg tctttgtgta
      421 tctccggaag gcttccagcc tgttttgcat gaaacagacc tataggcaac atttcaaaat
      481 cctcttcttg aagcacatca aaaaactctt ccggtaattt caacaacccc ttgttctgct
      541 tgatggaaat ttcccaaata cttgctgcac tgacaaagat cgcatttctc ggattttcta
      601 tcagtttgcg tgcagatatc cccagttcct tgtcatccaa caaccaccac agcaacgcat
      661 gggtatcaag cagaatcttt ctcacagagc cgactcctca aaaaataaag ctgccgtttc
      721 attgtcatcc tcaagaatac gtgaaatatc cgtattttcc atatgactga attttttcaa
      781 ccttcctgca tttcgtgccg gtttttcaat accgattagt tggacgcaag gcttacctgc
      841 cttcgcaata ataacaattt cccctgcttc tgctctttga atcaattgac tcaaattggt
      901 ttttgcctga tgaatatttg cttgaaacat aacacttctc atgattagct aacttaacta
      961 atatacatca ttaccaagat tttgggaatc tcattacata tatttgatta tatccgccgt
     1021 tttattcaca ccttg
//
LOCUS       DAWHSK010000352         1032 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.352, whole genome shotgun sequence.
ACCESSION   DAWHSK010000352 DAWHSK010000000
VERSION     DAWHSK010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.352"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            169..792
                     /gene="lolA"
                     /locus_tag="WIC03_002109"
     CDS             169..792
                     /gene="lolA"
                     /locus_tag="WIC03_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HEZ3884556.1"
                     /translation="MMKPHNLFQFLAVCSLTVSVASAQAGAVDALKQFNNDADGISGS
                     FTQTVQSKKKTQTAHGTFKILRPGLFKWEYTSPYKQTIVGDGQTVWLYDVDLAQVTKS
                     SQDQAIGGSPAAILSNKTALESSYTLKEDGSSNGIDYVLATPKRNNAGYQYIRIGFKG
                     GNLAAMQLKDSFGNQTSISFGGLNTNPQLSRGAFKFTPPKGVDVLSN"
BASE COUNT          257 a          346 c          247 g          182 t
ORIGIN      
        1 cggcacacat taaagggcag cgtggcgcgc ctgccttttc cgagcggtca aaaaaatcag
       61 ccctcggaaa acgctgtttg taaaatgcaa accgcccgta acgccgcccg tatgattgtt
      121 ttgccgcgcc gatactttac gccacaccca tcccaacaag gaaaaataat gatgaaaccg
      181 cacaacctgt tccaatttct cgccgtttgc tccctgaccg tctccgtcgc ttccgcacag
      241 gcgggcgcgg tggacgcgct caagcaattc aacaacgatg ccgacggtat cagcggcagc
      301 ttcacccaaa ccgtccaaag caaaaagaaa acccaaaccg cgcacggcac gttcaaaatc
      361 ctgcgcccgg gcctcttcaa atgggaatac acttcgccct acaaacagac tattgtcggc
      421 gacggtcaaa ccgtttggct ctacgatgtc gatttggcac aagtgaccaa gtcgtcccaa
      481 gaccaggcca taggcggcag ccccgccgcc atcctgtcga acaaaaccgc cctcgaaagc
      541 agctacacgc tgaaagagga cggttcgtcc aacggcatcg attatgtgct ggcaacgccc
      601 aaacgcaaca acgccggcta ccaatacatc cgcatcggct tcaaaggcgg caacctcgcc
      661 gccatgcagc ttaaagacag cttcggcaac caaacctcca tcagtttcgg cggtttgaat
      721 accaatcccc aactctcgcg cggcgcgttc aagtttaccc cgcccaaagg cgtggacgtg
      781 ttgagcaact gatgccgtcc gccccgatgc cgtctgaaag ccgccgaggc ttcagacggc
      841 attttcacgc aggcggaaca atgtcccgca ttaccgcccg atcgggcatc ggaacggcaa
      901 acccgtgaaa attaacggtt gcgccccggc tgtttttgcc gtttaatgca aaccttgctg
      961 caccaagggc caagaaagcc gaccggccgc ccccacagct tccgatgcag gcggcccggc
     1021 cgtccctgca at
//
LOCUS       DAWHSK010000353         1032 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.353, whole genome shotgun sequence.
ACCESSION   DAWHSK010000353 DAWHSK010000000
VERSION     DAWHSK010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.353"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..609
                     /gene="purH"
                     /locus_tag="WIC03_002110"
     CDS             <1..609
                     /gene="purH"
                     /locus_tag="WIC03_002110"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360677.1"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEZ3884557.1"
                     /translation="DGETVKQITDNQFMEVLMAPKFTAEALEIAAAKKNVRVLEVPLE
                     AGANRFELKRIGGGLLVQTPDIHRISRADLKVVSKRQPTEQEWNDLLFVWNVAKYVKS
                     NAIVFGKGGQTYGIGAGQMSRVDSTRIAARKAQDANLDLNGACAASDAFFPFRDGVDV
                     IAEQGIKAIIHPAGSMRDQEVFDAADEHGIAMVVTDVRHFRH"
BASE COUNT          252 a          292 c          295 g          193 t
ORIGIN      
        1 gacggcgaaa ccgtcaaaca gattaccgac aaccagttta tggaagtctt gatggcgccg
       61 aagtttaccg ccgaagccct cgaaatcgcc gccgccaaga aaaacgtgcg cgtattggaa
      121 gtgccgcttg aggcaggcgc aaaccgcttc gaactcaaac gcatcggcgg cggactgttg
      181 gtacaaaccc ccgacatcca ccgcatcagc cgcgccgatt tgaaagtcgt ctccaaacgc
      241 caaccgaccg agcaggaatg gaacgatttg ctgtttgtct ggaacgtcgc caaatacgtc
      301 aaatccaacg ccatcgtctt cggcaaaggc ggtcaaacct acggcatcgg cgcaggtcaa
      361 atgagccgcg tggacagcac tcgcatcgcc gctcgcaaag cgcaagatgc caatctcgac
      421 ctcaatggcg catgtgccgc ttccgatgct ttcttcccct tccgcgacgg cgtagatgtc
      481 attgccgagc agggcatcaa agccatcatc catccggcag gctccatgcg cgatcaggaa
      541 gtcttcgacg cagcggacga acacggcatc gccatggtcg taaccgacgt gcgccacttc
      601 cgccattaac gcagatgaac aaggcaatgc cgtctgaagg gctttcagac ggcattttgc
      661 gctattttgt gaaggtaggg atgacggttc gggtattcct gacagggtgg attttcaagg
      721 tgttgtatag ggtgtaggag gattcgtaaa aggtgggatg cagggtgtgc ttcagcccgc
      781 tgcatcaaaa atttttggag aaccggcggg agtcggcggt tttggtttcg gcggggacgg
      841 tggaaatggg taacattgac ggaatcgacg gaagcggtgg gctgaagccc acccttgtat
      901 attggaatca ctgtatcata gcaacaaacc gcccggccgc cacccgcgcc cacccaaggc
      961 acacaaccgt tgcgtagcac agggagcggc agggcaaccc atcgacacaa ccggacagtt
     1021 gccggacaac ac
//
LOCUS       DAWHSK010000354         1025 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.354, whole genome shotgun sequence.
ACCESSION   DAWHSK010000354 DAWHSK010000000
VERSION     DAWHSK010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.354"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(107..979)
                     /gene="hpnD"
                     /locus_tag="WIC03_002111"
     CDS             complement(107..979)
                     /gene="hpnD"
                     /locus_tag="WIC03_002111"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HEZ3884558.1"
                     /translation="MKGLDYCRQKAEESRSSFLSGFRFLTQKKRDAVTVLYAFCRELD
                     DVVDECSNPDVAQATLNWWRGDLDKVFGGEMPEHPVNQALQQVKETFKLPKYELEALI
                     DGMQMDLVQARYGSFEELKLYCHRVAGVVGCLIARILGFSDSKTLEYADKMGLALQLT
                     NIIRDVGEDARNGRIYLPMEEMRRFDVPASVILQCRPTENFTELMAFQIKRARETYRE
                     AVSLLPDADKKAQKVGLVMAAVYYALLNEIDRDGAQNVLKYKIALPSPRKKRIALKTW
                     LFGFKPRPGTPERA"
BASE COUNT          215 a          269 c          270 g          271 t
ORIGIN      
        1 tcgggcgcgg atgcggcgtg ttcatcattt attcctccaa tggttgaaac cccgtttttc
       61 ggggcggcgg gggttgggtt ttcctgaagg gcgggcggta tgcgccttat gcccgttccg
      121 gcgtgccggg gcgcggtttg aatccgaata accaggtttt cagggcaatg cgttttttgc
      181 gcggcgaagg gagggcgatt ttgtatttga ggacgttttg cgcgccgtct cggtcgattt
      241 cgtttaaaag cgcgtaataa accgccgcca taaccagtcc gactttttgg gcttttttgt
      301 cggcatcagg cagcagcgat acggcttcgc ggtaggtttc acgcgcacgc tttatttgga
      361 atgccatcaa ttcggtgaaa ttttctgtcg gccggcactg caaaatcaca cttgcgggga
      421 catcaaaccg ccgcatttcc tccatcggca ggtaaatccg cccattacgg gcgtcttcgc
      481 cgacatcgcg gatgatgttg gtcagttgca gcgcaagtcc cattttgtcg gcgtattcca
      541 gtgttttgct gtctgaaaac cccaaaatcc gcgcaatcag gcagccgact acgcctgcga
      601 cgcggtggca atacagtttc aattcttcaa aactgccgta acgggcttga accaaatcca
      661 tctgcatccc gtcgattaag gcttccagtt catatttcgg cagcttgaag gtttccttaa
      721 cctgctgcaa ggcctgattg acggggtgtt ccggcatctc gccaccgaac accttgtcca
      781 aatcgccgcg ccaccagttc aatgttgcct gtgcaacatc ggggttggaa cattcgtcaa
      841 ccacatcgtc caattcgcgg caaaaagcat ataaaaccgt taccgcatcc cgttttttct
      901 gagtcaggaa acggaagccc gataaaaaac tggagcggct ttcttctgct ttttgacggc
      961 aatagtcgag tcctttcacg atttatattc ctaatgatgg gcgagaaaga cggattttat
     1021 cggca
//
LOCUS       DAWHSK010000355          988 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.355, whole genome shotgun sequence.
ACCESSION   DAWHSK010000355 DAWHSK010000000
VERSION     DAWHSK010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..988
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.355"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(17..955)
                     /locus_tag="WIC03_002112"
     CDS             complement(17..955)
                     /locus_tag="WIC03_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884559.1"
                     /translation="MDAYTEAEAPPQSIVELVPVLIAVTDGGLRVLTVAQGTLLPNGP
                     LSPLRNSLQAGVKLWVAKQTSQPMGYVEQLYTFVDTHRRNEHGMPVLYVSYLGLVREA
                     ADSILHPDAKWQDCYGYFPWEDLRTDGGQRDAVVSRLRIWANSADTEEVRQMRLKRIH
                     LCWGVEPENWSEEYVLQRYEMLYESGLIVEAAEPQANFDFALTGQPMRHDHRRVLATA
                     LSRLRAKIKYRPVIFELMPPEFTLLQLQNSVEAISGRLLHKQNFRRQIQQQNLIEPSD
                     TGVSGSKGRPAQLCRFRDDVLPDRLISDIGLPLGSR"
BASE COUNT          182 a          280 c          315 g          211 t
ORIGIN      
        1 gtcgtctgaa aacgggctaa cggctgccca gcggcaatcc gatgtccgaa atcagcctgt
       61 cgggcaggac gtcgtcgcgg aagcggcaaa gctgcgcggg acggcctttg ctgcccgata
      121 cgccggtatc cgacggctcg ataaggtttt gctgctgaat ctggcggcgg aagttttgct
      181 tgtgcagcaa tcttccgctg atggcttcga cactgttttg cagttgcagc agcgtgaatt
      241 cgggcggcat cagttcaaaa atcacggggc ggtatttgat tttggcgcgc aggcgggaca
      301 gggcggtcgc cagtacgcga cggtgatcgt ggcgcatggg ctgccccgtg agcgcgaagt
      361 cgaagtttgc ctgcggctcg gcggcttcca ctatcagacc gctttcatac agcatttcat
      421 agcgttgtaa aacgtattct tccgaccagt tttccggttc gaccccccag cacaaatgaa
      481 tgcgcttgag ccgcatttga cgcacttcct ccgtgtccgc cgaatttgcc caaatgcgca
      541 ggcggctgac gacggcgtcg cgctgcccgc cgtcggtgcg caagtcttcc cacgggaaat
      601 agccgtagca gtcctgccat ttcgcatccg ggtgcaggat gctgtcggct gcctcgcgca
      661 ccagccccaa atagctgacg tacaagacgg gcatgccgtg ttcgttgcgg cggtgggtat
      721 cgacaaaggt gtaaagctgt tccacatagc ccataggctg cgaagtctgc ttggcaaccc
      781 acagcttcac gcccgcctgc agggaattgc gcagagggga gagcgggccg ttgggcagga
      841 gtgtgccttg ggcgacggtt aatacccgca ggccgccgtc ggtaacggca atcaatacgg
      901 gaaccagctc gacgatgctt tgcggcgggg cttcggcttc ggtgtaggca tccatgctcg
      961 gctttctgcg ggtgggaaag gctttatt
//
LOCUS       DAWHSK010000356          977 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.356, whole genome shotgun sequence.
ACCESSION   DAWHSK010000356 DAWHSK010000000
VERSION     DAWHSK010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..977
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.356"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(148..804)
                     /locus_tag="WIC03_002113"
     CDS             complement(148..804)
                     /locus_tag="WIC03_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEZ3884560.1"
                     /translation="MKNKTKVWDFPTRLFHWLLAASLPFMWYSAKTGGDMLQWHTRVG
                     LFILFLLVFRLCWGIWGSDTARFSRFVRGWSGIREYMKNGIPEHVQPGHNPLGALMVV
                     ALLAAVSFQVGTGLFAADENTFSTNGYLNHLVSEHTGSLMRKIHLNFFKLLAVFSAIH
                     IAAVAAYRVFKKKNLVLPMITGFKYIEGKTSIRFAGKTALAAALSVAALAAAAILLLS
                     "
     gene            869..>977
                     /locus_tag="WIC03_002114"
     CDS             869..>977
                     /locus_tag="WIC03_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEZ3884561.1"
                     /translation="MFPVFHLSGESRRRMLQAASRFPYVFKARAEDSHKG"
BASE COUNT          224 a          221 c          310 g          222 t
ORIGIN      
        1 ccttgtcctg atttttgtta atccactata aaacggtttg ggacggaaaa acggcctgaa
       61 gccgtttcgg gcattcagac cgtttgcgcg gagaggggat gccgtccgaa gggcgaaaag
      121 ggcttcagac ggcattgatg tcgggtttca ggacaggagc aggatggcgg ctgcggcaag
      181 cgcggcaacc gataatgcgg cggcaagcgc ggttttgccg gcaaagcgga ttgaggtttt
      241 gccttcgatg tatttgaagc cggttatcat cgggaggacg aggtttttct ttttgaacac
      301 gcggtatgcg gcgacggcgg cgatatgaat ggcggaaaaa acggcgagca gcttgaaaaa
      361 gttgaggtgg attttccgca taaggctgcc cgtatgctcg gaaaccaaat ggttgaggta
      421 gccgttggtg ctgaaggtgt tttcatcggc ggcaaaaagc cctgtgccga cttgaaatga
      481 gacggcggcc aaaagcgcga cgaccatcag tgcgcccaag gggttgtgtc cgggttggac
      541 gtgttcggga ataccgtttt tcatatactc tctgataccc gaccatccgc ggacgaaacg
      601 ggagaaacgg gcggtatcgc tgccccaaat gccccagcag aggcggaata cgagcaggaa
      661 aaggataaac agcccgacgc gcgtgtgcca ttgcagcata tcgccgccgg ttttcgcgct
      721 ataccacata aagggtaggg atgcggcaag cagccagtgg aaaaggcggg tggggaagtc
      781 ccagactttg gttttgtttt tcataattgg tttccggcgg tagaatcggt ttgttttcga
      841 gccttaattt taaccgattg gaggggaaat gtttcccgtt ttccatcttt caggcgaaag
      901 ccgccgccgg atgcttcagg cggcatcgcg ttttccctat gttttcaaag cccgtgcgga
      961 agattcgcac aaaggga
//
LOCUS       DAWHSK010000357          968 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.357, whole genome shotgun sequence.
ACCESSION   DAWHSK010000357 DAWHSK010000000
VERSION     DAWHSK010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..968
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.357"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(9..200)
                     /locus_tag="WIC03_002115"
     CDS             complement(9..200)
                     /locus_tag="WIC03_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884562.1"
                     /translation="MDFQFFVETIRRQEKKLKHGVYRLFEIYSWLEDGKATKARIRYF
                     VKIEVDKQILRRIHRGIAD"
     gene            329..889
                     /locus_tag="WIC03_002116"
     CDS             329..889
                     /locus_tag="WIC03_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA large subunit pseudouridine synthase E"
                     /protein_id="HEZ3884563.1"
                     /translation="MKNLIAFNKPYGVICQFSPHEKHKSLKDFINLPGFYPAGRLDTD
                     SEGLLLLTDDGRLQAQITDPKFKHPKTYWAQLEGVPDESRLESLRKGIDLGGFVTRPA
                     SIRILKHGEADSLWERIPPIRVRKTVPDFWIEITISEGKNRQVRRMTAKAGYPCLRLI
                     RVASGRLKLFDLDLKPGEWAYAPFKP"
BASE COUNT          275 a          267 c          194 g          232 t
ORIGIN      
        1 ggtttttgtt aatccgctat accacgatga atccttcgca atatctgttt atcgacctca
       61 attttgacaa aataccggat acgcgccttt gttgcttttc catcttccaa ccaactgtaa
      121 atctcaaaca gccggtacac gccatgcttc agtttctttt cctgtcggcg gattgtttcg
      181 acaaagaatt gaaaatccat ttcatgcacc ttaaaattta atctgcattc aaaccttttc
      241 actttggaag caccatttat cggatgtccc ttcgcaataa acaaattttc ccgataccgc
      301 cgcccatttc aacccaaacc caaaacctat gaaaaacctc atcgccttca acaaacccta
      361 tggcgttatc tgccaatttt caccgcacga aaaacacaaa agcctcaaag actttatcaa
      421 tcttcccggc ttctaccccg ccggacggct cgacaccgac agcgaggggc tgctgctgct
      481 gaccgacgac ggcaggcttc aggcacaaat taccgacccc aaattcaaac accctaaaac
      541 ctactgggcg caactggagg gcgtacccga cgaaagccga ttggaaagcc taagaaaagg
      601 gatagactta ggcggtttcg ttacccgtcc ggcaagcatc cgcatcttga aacacggaga
      661 agcagattcg ttatgggagc gcatcccgcc gatacgcgtc cgcaaaaccg ttcccgattt
      721 ttggattgaa attaccattt ctgagggcaa aaaccgccaa gtcaggcgaa tgaccgccaa
      781 ggcgggctat ccctgcctgc gtctgatcag agtggcaagc ggcaggctga aactgtttga
      841 tttggattta aaacccgggg aatgggcata cgccccgttt aaaccataat cacgtttatc
      901 tcatcatttc cacaaaagtg ggaatccgga attttatagt ggattaacaa aaatcaggac
      961 aaggcgac
//
LOCUS       DAWHSK010000358          948 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.358, whole genome shotgun sequence.
ACCESSION   DAWHSK010000358 DAWHSK010000000
VERSION     DAWHSK010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..948
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.358"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..152
                     /locus_tag="WIC03_002117"
     CDS             <1..152
                     /locus_tag="WIC03_002117"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242742.1"
                     /note="catalyzes the formation of L-threonine from
                     O-phospho-L-homoserine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEZ3884564.1"
                     /translation="FDATIREAVGDAAIPRPAALEGLETLPQRVQTVPNSADAVKGII
                     EQTLA"
     gene            199..>948
                     /locus_tag="WIC03_002118"
     CDS             199..>948
                     /locus_tag="WIC03_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884565.1"
                     /translation="MTLPMQETRFSILLDELAAKQEAAIAPHLLADGSKVWIRKAGRH
                     NARWRYALLGMVARYLKLGVLKPVPSLGGEPAIATESKRLYELRAAGIAVPELLAVRK
                     NALMFGNLEGIPLDTQIRQEAEAGKSDAWLAGLEAIARVHKKRQFLSQAFARNMMSDG
                     KNISFLDFEDDPSEVLTIAQCQARDWLCYIHSTALILKNGGLLEAAAEKWGGVLSDQP
                     AEIQKLIAATVKPILPIRRLEHPRWGRDALRL"
BASE COUNT          230 a          268 c          270 g          180 t
ORIGIN      
        1 aattcgatgc gaccatacgc gaagccgtcg gcgatgccgc cattccgcgc cccgccgcgc
       61 tggaaggttt ggaaacattg ccgcagcgcg tgcaaaccgt gccgaacagt gcggatgcgg
      121 taaaaggcat catcgaacaa acccttgcct gatgttccgt atgggcggca ttccgtccga
      181 acttaagatt gaaagaaaat gaccttacct atgcaggaaa cccgtttttc catactgctg
      241 gacgaattgg cggcaaaaca ggaggcagcc atcgcccccc acctgcttgc cgacgggagc
      301 aaggtatgga tacgcaaagc aggcaggcac aatgcacgat ggcgttacgc gctgctcggt
      361 atggttgccc gatatttgaa actgggggtg ttgaagccgg tccccagcct cggcggcgag
      421 cctgccattg caaccgaatc aaaacgcctg tacgaattgc gcgcggcggg gatagccgtt
      481 cccgaattgc tcgccgtccg gaaaaatgcc cttatgttcg gcaatttgga aggcatcccg
      541 ctcgatacgc aaatccgcca agaagccgag gctggaaagt cggacgcttg gcttgccggt
      601 ttggaagcca ttgcacgcgt gcataaaaaa cggcagttcc tcagtcaggc gtttgcgcga
      661 aatatgatgt cggacggaaa aaacatcagc tttttggatt ttgaagacga cccttccgaa
      721 gtcctgacca tagcgcaatg ccaagcccgc gattggctct gctacatcca ttcgaccgcg
      781 ctgatattga aaaacggcgg actgctggaa gcggctgcgg aaaaatgggg cggcgtattg
      841 tcagatcagc ctgccgaaat acaaaagctg attgccgcca ccgtcaaacc gatcctcccg
      901 atacgcaggc tggaacaccc ccgctgggga cgggacgcac tcaggctg
//
LOCUS       DAWHSK010000359          948 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.359, whole genome shotgun sequence.
ACCESSION   DAWHSK010000359 DAWHSK010000000
VERSION     DAWHSK010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..948
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.359"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..907
                     /locus_tag="WIC03_002119"
     CDS             <1..907
                     /locus_tag="WIC03_002119"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEZ3884566.1"
                     /translation="YESRPNVTSDAAALALKSGSAVVLRSGKDAFQSARAIVAALKAG
                     LEQTRIDPDALQLIEDTGREGSYEMMRAKDYLDLLIPRGGAGLIRAVVENAVVPVIET
                     GTGIVHIYIDKDADWDKALRIVYNAKTSRPSVCNSMEVLLVHEDIAADFLPKLERLLV
                     RDRIEAGLPPVRFRLDPQAARHIGGEAAGADDFDTEFLDYILAVKTVASVEEAVWHIE
                     THSTHHSDGIVTENRHAADYFTTHIDSAAVYVNASTRFTDGGEFGLGCEMGISTQKLH
                     ARGPMGLKELTSYKYIVQGTGQVRE"
BASE COUNT          222 a          267 c          275 g          184 t
ORIGIN      
        1 ttacgaaagc cgcccgaacg ttacgtccga tgcggcggct ttggcactga aaagcggcag
       61 cgcggtcgta ctccgcagcg gcaaagatgc attccaatcc gcacgcgcca tcgttgccgc
      121 actcaaagcc ggtttggagc aaacccgcat cgaccccgac gcgctccagt tgattgaaga
      181 cacagggcgt gagggcagtt acgaaatgat gagggcgaaa gattatctag acctgctgat
      241 tccgcgcggc ggggcggggc tgatacgggc ggtggttgaa aatgccgtcg tgccggtcat
      301 tgaaacggga acgggcattg tccacattta tatcgacaag gacgcggatt gggacaaggc
      361 gctccgtatc gtgtacaacg ccaaaaccag ccgtccgtcc gtgtgcaact cgatggaagt
      421 gctgctggta catgaagaca ttgctgccga cttcctgccc aagctcgaac ggctgttggt
      481 tcgcgaccgc atagaagccg gactgccgcc cgtccgcttc cgtttggatc cgcaggcggc
      541 gcggcatatc ggcggcgaag cggcgggtgc agacgatttc gataccgagt ttttagacta
      601 catcctcgct gtgaaaaccg tcgcttcggt cgaagaggcg gtctggcaca tcgaaaccca
      661 cagcacacac cattcagacg gcatcgttac cgaaaaccgc cacgctgcgg actatttcac
      721 gacccatatc gattctgccg ccgtgtatgt caacgcgtcc acgcgcttta ccgacggcgg
      781 cgaattcggc ttgggttgcg aaatgggcat ctccacgcaa aaactccacg cccggggccc
      841 gatgggtttg aaagagctga cgagttacaa atacatcgta caaggcacgg gacaggtcag
      901 ggaataatcc gaattcaaat gtctgatata gtggattaac tttaaacc
//
LOCUS       DAWHSK010000360          939 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.360, whole genome shotgun sequence.
ACCESSION   DAWHSK010000360 DAWHSK010000000
VERSION     DAWHSK010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..939
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.360"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..834)
                     /gene="mafB"
                     /locus_tag="WIC03_002120"
     CDS             complement(<1..834)
                     /gene="mafB"
                     /locus_tag="WIC03_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HEZ3884567.1"
                     /translation="MKLPIQKFMMLFAAAISLLQIPISHANGLDARLRDDMQAKHYEP
                     GGKYHLFGNARGSVKNRVYAVQTFDATAVGLILPITHERTGFEGIIGYETHFSGHGHE
                     VHSPFDNHDSKSTSDFSGGVDGGFTVYQLHRTGSEIHPEDGYDGPQGSDYPPPGGARD
                     IYSYYVKGTSTKTKTNIVPQAPFSDRWLKENAGAASGFFSRADEAGKLIWESDPNKNW
                     WANRMDDIRGIVQGAVNPFLMGFQGLGVGAITDSAVNPVTYAAARKTLQGIHNLGNLS
                     PE"
BASE COUNT          228 a          235 c          200 g          276 t
ORIGIN      
        1 ttccggactt aaatttccta aattgtgaat accctgtaaa gttttccgtg ctgccgcata
       61 ggttaccggg tttaccgcac tgtctgtaat tgccccaact cccaatccct gaaaacccat
      121 taaaaaagga ttaaccgcac cttggacgat gccgcgaata tcatccatac ggttagccca
      181 ccaattttta ttggggtcgc tttcccatat cagttttcct gcttcatccg cacggctgaa
      241 aaaaccagag gcggcaccgg cattttcttt tagccaacgg tctgaaaatg gggcttgagg
      301 gacaatatta gtctttgttt ttgttgaagt tcctttgaca taatagctgt atatatccct
      361 tgctcctccg gggggcggat aatcgctgcc ttgcggcccg tcatatccat cctccggatg
      421 aatttccgac cctgtccgat gaagttggta aacagtaaaa ccaccgtcta caccgccgct
      481 gaaatcagaa gtgctttttg aatcatgatt atcgaacgga ctgtgtactt catgtccatg
      541 tcctgaaaaa tgggtttcat aaccgataat gccttcaaac cctgtccgtt cgtgtgtaat
      601 aggcagtatg aggccgaccg cagttgcatc aaatgtctgg acggcgtaaa cccgattttt
      661 aacactgccg cgagcattac caaacagatg gtatttacca cccggttcgt agtgttttgc
      721 ctgcatatca tcgcgtaaac gggcatccaa accgttcgca tgactaatgg ggatttgcag
      781 caacgatatt gccgctgcaa acagcatcat gaatttttga ataggcaatt tcattttgtt
      841 ttccttggta gcaagcggtt atggtcgtgt gaatcaaggt tgcccttgtc tatgttgtcg
      901 cactacttca tcgctgtatc catacccctc atgactgtt
//
LOCUS       DAWHSK010000361          906 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.361, whole genome shotgun sequence.
ACCESSION   DAWHSK010000361 DAWHSK010000000
VERSION     DAWHSK010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.361"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(55..834)
                     /locus_tag="WIC03_002121"
     CDS             complement(55..834)
                     /locus_tag="WIC03_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HEZ3884568.1"
                     /translation="MKLVRPQKISDQVLSVLEERIAEGVYAEGGKIPPERVLAEEFGV
                     SRPSVRSALNVLVARQVLEARQGDGYYVSVKPQQDFLQSWQELLGKHSNWEQDVFDFS
                     CHIEGCMAALAAERRTDADLKRIGFWLEKFEEACESGNLEHQSEADVSFHQTIADAAH
                     NLLFSHLSGGLLKMLYRQTRSSLIYLNQEEDPRPKLMAQHRVLYEAISNRRPGEASEA
                     AKAHLNYVASSIMKDREYQSRNRHADTLAQNDLKRVQDWEV"
BASE COUNT          222 a          291 c          179 g          214 t
ORIGIN      
        1 aatttagttc actataccga tacatccgaa tatgccagac cgtctaaacg gtattcatac
       61 ctcccaatcc tgcacgcgct tcaaatcgtt ttgcgccaaa gtatccgcgt ggcggttacg
      121 gctctgatat tccctgtctt tcatgatgct gcttgccaca taattcaaat gtgcctttgc
      181 cgcctccgaa gcctcgcccg gccgacggtt cgatatcgcc tcatacagca cgcggtgctg
      241 cgccatcagt ttcggacgcg gatcttcttc ctgattcaga taaataaggc tgctgcgcgt
      301 ctgccggtac agcattttca acaaaccgcc cgacaaatgg ctgaacaaca aattgtgcgc
      361 cgcatccgca atcgtctgat ggaagctgac atcggcttcg ctctgatgtt ccagattgcc
      421 gctttcgcac gcctcctcaa acttttcaag ccaaaaccca atccgcttca aatcggcatc
      481 cgtccggcgt tctgccgcca atgccgccat acagccctcg atgtggcagc tgaaatcaaa
      541 aacatcctgt tcccaattgg aatgcttgcc caaaagctcc tgccaacttt gcaaaaaatc
      601 ctgctgcggc ttgaccgaaa cataataacc gtctccctgc ctcgcttcca aaacctgacg
      661 ggcaaccaaa acattcaatg ccgaccggac cgacgggcgc gaaacgccga actcttccgc
      721 caaaacgcgt tcgggcggaa tcttgccccc ttccgcgtaa accccttccg caatgcgctc
      781 ctccaatacc gacaatacct gatcgctgat tttctgaggc cttaccagtt tcatcactcc
      841 tcctttataa agattccctg cagaaccctt ccgaaatata gtggattaac aaaaatcagg
      901 acaagg
//
LOCUS       DAWHSK010000362          906 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.362, whole genome shotgun sequence.
ACCESSION   DAWHSK010000362 DAWHSK010000000
VERSION     DAWHSK010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.362"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(1..310)
                     /locus_tag="WIC03_002122"
                     /pseudo
     CDS             complement(1..310)
                     /locus_tag="WIC03_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611454.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(430..636)
                     /locus_tag="WIC03_002123"
     CDS             complement(430..636)
                     /locus_tag="WIC03_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884569.1"
                     /translation="MFDGAKVVRRMEGNLWVFGMVALEKTCFKGQMPSENRFQTAFSV
                     YLKQTGKGSNILQSSYSHKRFIVN"
BASE COUNT          233 a          248 c          170 g          255 t
ORIGIN      
        1 tatagccccg tccgttgcgt ttcagaatgc cgaagacaac cacttttcct gccgcaccgc
       61 gaccacgtct gcctttacgc cgtccgccga aatcgctttc gtccggctcg acagggccct
      121 caaaaacctc atcggcagcc aagaccaaat gatagttgat aaccgtgcgg attttgcggt
      181 agaacagtac tgccgaattg ggatggatac ccaaaatatc ggcggcagaa cgggcggtaa
      241 cttccagtac aaaaaaacgg agcagttctt tctgtacttt tttctttaat tttcagtgcg
      301 ttatcttcat atttcgaggg taacatatct gctaatctag tacagcccct agtttaaagc
      361 tttgtttctt aagtccgcag agtatgccat ggttagacct tcaaagttga gtattgtact
      421 attttgtttt taattaacta taaaacgctt gtgtgaatag gaagactgca gaatattgct
      481 gccttttcct gtttgcttta aataaacaga aaatgccgtc tgaaaccgat tttcagacgg
      541 catttgtccc ttaaaacacg ttttttcaag cgcgaccata ccaaacaccc acaggttgcc
      601 ttccatcctt cgtacaacct ttgctccatc aaacatccac ttcctaaata ctgatgtctg
      661 cttaggttga atcggcattc gtacgattac ggcacaccgc ctaatctgta cctgcgtctg
      721 ttgcgcccaa taccaaaggc cacctgaaaa tctcccaaac ttttcaggta gccttatcac
      781 gttttcgact gttaccacac gcccaaagac cgcctgaaac cctttttcag acgttccgct
      841 ccgatacccc ccaacaaaac gccgcccgaa cgcatgttca gacggcattt tctttgcgtc
      901 gattca
//
LOCUS       DAWHSK010000363          905 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.363, whole genome shotgun sequence.
ACCESSION   DAWHSK010000363 DAWHSK010000000
VERSION     DAWHSK010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..905
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.363"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..215
                     /locus_tag="WIC03_002124"
     CDS             <1..215
                     /locus_tag="WIC03_002124"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884570.1"
                     /translation="DAGDVYCVYNIRLKQYTACQVTRVEEHGGKKTRATLLALDWQGN
                     KPLGAAELADLKPLYKDLKNNIGNIV"
     gene            546..>905
                     /locus_tag="WIC03_002125"
     CDS             546..>905
                     /locus_tag="WIC03_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884571.1"
                     /translation="MYWERGLHMYKASAVVPTGYVRVGNTAPLVGEDTQRYASFWGDG
                     YDVYRQLRWQQIPEKQRKAFKKAAKSKKTVMFAGREYGISKQNLSDVWDDFEDAMELK
                     AFPCLSSLFLTKWHKNLY"
BASE COUNT          280 a          149 c          212 g          264 t
ORIGIN      
        1 ctgatgccgg agacgtatat tgcgtctata acatcagact gaagcagtac actgcctgcc
       61 aggttacccg agttgaagaa cacggtggca aaaaaacacg tgcgaccctg ctggctttgg
      121 actggcaggg caacaaaccg cttggggcgg cggaactggc ggatttgaaa ccgctttaca
      181 aagacttaaa gaataatatt ggaaatattg tatgaacaaa aaattaaact atatttttat
      241 gttggactgt ttagggttgg tgatattgtt tacttgtata atagctactt ttgaaagaga
      301 ttatggattt aaaattttta ctaattctaa gaggcctgaa ttttattatt ggattggaat
      361 gttttattat ggaattattt cttgctggtt tgattatcaa ttaatttcaa caaaggcgaa
      421 ttcgtataaa agaaaagtta aacaatataa aattttttca gtaatatttt cagttttgat
      481 atttatttct actatagtaa aactttaaat tttggagcaa aaatttatga gcgattcaat
      541 tgaatatgta ttgggaacgc ggtctgcaca tgtataaggc aagtgccgtc gtgccgacgg
      601 gatatgtacg ggttgggaat accgcgccgc tggtcggcga agacacgcaa cggtatgcct
      661 ctttttgggg cgacggctac gacgtgtacc gtcagttgag atggcagcag atacccgaaa
      721 aacagagaaa ggcattcaaa aaagccgcca aaagcaaaaa gaccgtgatg tttgccggac
      781 gggaatacgg catatccaaa cagaatttga gcgatgtttg ggatgatttt gaagacgcga
      841 tggaactgaa ggcgtttccc tgcctgtctt cgctgtttct gaccaaatgg cataaaaatc
      901 tatat
//
LOCUS       DAWHSK010000364          905 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.364, whole genome shotgun sequence.
ACCESSION   DAWHSK010000364 DAWHSK010000000
VERSION     DAWHSK010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..905
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.364"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(87..719)
                     /gene="mtrR"
                     /locus_tag="WIC03_002126"
     CDS             complement(87..719)
                     /gene="mtrR"
                     /locus_tag="WIC03_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux system transcriptional
                     repressor MtrR"
                     /protein_id="HEZ3884572.1"
                     /translation="MRKTKTEALKTKEHLMLAALETFYRKGIARTSLNEIAQAAGVTR
                     GALYWHFKNKEDLFDALFQRICDDIENCIAQDAEDAEGGSWAVFRHTLLHFFERLQSN
                     DIYYKFHNILFLKCEHTEQNAAVIAIARKHQAIWREKITAVLTEAVENQDLADDLDKE
                     TAVIFIKSTLDGLIWRWFSSCERFDLGKTAPRIIGIMMDNLENHPDLRRK"
BASE COUNT          201 a          203 c          237 g          264 t
ORIGIN      
        1 tgtattttta gaagaaggct ttgtgtcatt ttgatgccgt ctgaaagggt ttgttcgttt
       61 cagacggcat tgctaccaag gcttgattat ttccggcgca ggtcgggatg gttttccaag
      121 ttgtccatca ttatcccgat gatgcgcggg gcggttttgc ccaaatcgaa acgttcgcag
      181 gaagagaacc accgccaaat cagcccgtcc aaggttgatt tgatgaagat aactgccgtt
      241 tccttgtcca aatcgtcagc caaatcctga ttttccaccg cttcggtcaa aacggcggta
      301 attttctcgc gccagattgc ctgatgcttg cgggcaatgg cgataacggc ggcgttttgc
      361 tccgtgtgtt cgcattttaa aaacaggatg ttgtggaatt tgtagtagat gtcgttgctt
      421 tgcagccgct cgaaaaagtg cagcagcgtg tggcggaata ccgcccaaga ccctccttcg
      481 gcatcttcgg catcttgcgc gatgcagttt tcgatgtcgt cgcagatacg ttggaacagc
      541 gcgtcgaaca agtcttcctt atttttgaaa tgccaataga gcgcgccgcg cgttacgccg
      601 gcggcttggg cgatttcgtt gagcgaggtg cgcgcaatcc ctttgcggta aaaggtttcc
      661 aaggcggcaa gcatcaggtg ttctttggtt ttcaaggctt cggttttggt ttttctcata
      721 atggcgtttt cgtttcgggt cggtttgatg agggcggatt ataaaaaaga ctttgtaacc
      781 atgcaatcgt gtatgtataa tgaaacccat gaaattgaga ctacatctca actttgaaaa
      841 caggcataac ctgctttgca acccgttcga acattcgggt atcaaagcag gcggttttta
      901 tatcc
//
LOCUS       DAWHSK010000365          891 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.365, whole genome shotgun sequence.
ACCESSION   DAWHSK010000365 DAWHSK010000000
VERSION     DAWHSK010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..891
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.365"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..>891
                     /gene="tuf"
                     /locus_tag="WIC03_002127"
     CDS             <1..>891
                     /gene="tuf"
                     /locus_tag="WIC03_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEZ3884573.1"
                     /translation="MITGAAQMDGAILVCSAADGPMPQTREHILLARQVGVPYIIVFM
                     NKCDMVDDAELLELVEMEIRDLLSSYDFPGDDCPIVQGSALKALEGDAAYEEKIFELA
                     AALDSYIPTPERAVDKPFLLPIEDVFSISGRGTVVTGRVERGIIHVGDEIEIVGLKET
                     QKTTCTGVEMFRKLLDEGQAGDNVGVLLRGTKREDVERGQVLAKPGTITPHTKFKAEV
                     YVLSKEEGGRHTPFFANYRPQFYFRTTDVTGAVTLEEGVEMVMPGENVTITVELIAPI
                     AMEEGLRFAIREGGRTVGAGV"
BASE COUNT          224 a          234 c          242 g          191 t
ORIGIN      
        1 atgattaccg gcgccgcaca aatggacggt gcaatcctgg tatgttccgc agccgacggc
       61 cctatgccgc aaacccgcga acacatcctg ctggcccgtc aagtaggcgt accttacatc
      121 atcgtgttca tgaacaaatg cgacatggtc gacgatgccg agctgttgga actggttgaa
      181 atggaaatcc gcgacctgct gtccagctac gacttccccg gcgacgactg cccgattgta
      241 caaggttctg cactgaaagc cttggaaggc gatgccgctt atgaagagaa aatcttcgaa
      301 ctggctgccg cattggacag ctacatcccg actcccgagc gtgccgtgga caaaccgttc
      361 ctgctgccta tcgaagacgt attctctatt tccggtcgtg gtacagtagt aaccggtcgt
      421 gtagagcgcg gtatcatcca cgttggtgac gagatcgaaa tcgtaggtct gaaagaaact
      481 caaaaaacca cttgtaccgg tgttgaaatg ttccgcaaac tgctggacga aggtcaagca
      541 ggcgacaacg taggcgtatt gctgcgcggt accaaacgtg aagacgtaga gcgtggtcaa
      601 gtattggcta aaccgggtac aatcactcct cacaccaagt tcaaagcaga agtatacgta
      661 ctgagcaaag aagagggtgg ccgccatacc ccgttcttcg ctaactaccg tccgcaattc
      721 tacttccgta ccaccgacgt aactggcgcg gttactttgg aagaaggtgt agaaatggta
      781 atgccgggtg aaaacgtaac catcaccgta gaactgattg cgcctatcgc tatggaagaa
      841 ggtttgcgct ttgcgattcg cgaaggcggc cgtaccgtgg gtgccggcgt g
//
LOCUS       DAWHSK010000366          885 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.366, whole genome shotgun sequence.
ACCESSION   DAWHSK010000366 DAWHSK010000000
VERSION     DAWHSK010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..885
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.366"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(28..699)
                     /locus_tag="WIC03_002128"
     CDS             complement(28..699)
                     /locus_tag="WIC03_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ3884574.1"
                     /translation="MFPDKYKLSLKENIFLAKKVLVAQIHNLSRFENCQTTLLQTEQI
                     IHGKNVASASLEDIQTILNLKRAYQYVISHISNGEPVDISLLKKINNTVAKDDSLAPG
                     DFRTGSVGVTLLDGSRHAPNPVKEIEVARVLQNIGLQSGSTTEAAVRFMLYCMRQQVF
                     WDGNKRTATLFANGLMMERGRGILEISEMQMPQFNEKLSAFYRSGDDTDISKFVYQNC
                     ISGID"
BASE COUNT          235 a          198 c          199 g          253 t
ORIGIN      
        1 ggggttttgg ggaattttgc aaaggtctca gtctatgccc gatatacaat tttgatacac
       61 aaacttggaa atatcggtat cgtcgccgga gcgatagaat gcggacagtt tttcattgaa
      121 ttgcggcatc tgcatttcgg agatttccaa gatgccacgg cctctctcca tcatcagacc
      181 attggcaaat aaggttgccg ttcgtttgtt gccgtcccaa aaaacctgct gccgcataca
      241 ataaagcata aaacggacgg ctgcctccgt cgtcgaaccg ctttgcagtc cgatattttg
      301 taacacgcgg gccacttcaa tttccttcac tggattcggg gcatgacggg aaccgtccaa
      361 tagcgttacg ccgaccgaac cggtacggaa atcaccgggt gccaaagaat cgtccttggc
      421 aacagtgttg ttgatttttt taaggagtga aatatcgacc ggttcgccgt ttgaaatatg
      481 cgaaatcaca tattgatagg cacgtttcag gttcaagatg gtttggatgt cttccagtga
      541 cgcggaggct acatttttgc catggataat ttgttcggtc tgcaacaagg tcgtctgaca
      601 attctcaaaa cggctgaggt tgtgaatttg ggcaaccaat actttctttg ccaaaaaaat
      661 attttctttc aaactcaact tgtatttgtc gggaaacacg ataattcctt aaataattaa
      721 agcagtacgt taaatcatca tcttatcatc actttcgggg tttatgttgc gtgcgtccgt
      781 tttctaatac ggtttcccat cccgcgaaac gaggcggagg cctgtgcaaa aaacctgttc
      841 acgaaatttt ccgcattaac gatacgctga aaatgcgcta aaaat
//
LOCUS       DAWHSK010000367          858 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.367, whole genome shotgun sequence.
ACCESSION   DAWHSK010000367 DAWHSK010000000
VERSION     DAWHSK010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..858
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.367"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(78..154)
                     /locus_tag="WIC03_002129"
     tRNA            complement(78..154)
                     /locus_tag="WIC03_002129"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(118..120),aa:Pro,seq:cgg)
     gene            complement(236..>858)
                     /gene="nagZ"
                     /locus_tag="WIC03_002130"
     CDS             complement(236..>858)
                     /gene="nagZ"
                     /locus_tag="WIC03_002130"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEZ3884575.1"
                     /translation="LALALQKGLEKGGMKSCGKHFPGHGFVEGDSHLVLPEDGRSLSE
                     LETADLAPFHIMSREGMAAVMPAHVVYPQVDTKPAGFSEIWLKQILRRDIGFKGVIFS
                     DDLTMEGACGAGGIKERARISFEAGCDIVLVCNRPDLVDELREDFRIPDNPALAQRWQ
                     YMANTLGSAAAQAVMQTADFQEAQAFVAGLASPQDTAGGVKVGEAF"
BASE COUNT          180 a          234 c          249 g          195 t
ORIGIN      
        1 ctgtttcaga cggcattttt ctgttgccgc caaaaggaaa aaccgctccg gcaatggatg
       61 ccgaggcggt ttggatttgg tcggaatgag aggattcgaa cctccgaccc cttcgtcccg
      121 aacgaagtgc gctaccgggc tgcgctacat tccgaattaa gttaaggcgt gattatagcg
      181 caaaaaatac ggcgtgccta taccgttttg cctttttgca gcgtgtcggg cggatttaaa
      241 aggcttcgcc gacttttacg ccgcccgccg tgtcttgcgg cgaggcaagg ccggcaacaa
      301 aggcttgcgc ctcttggaaa tccgccgtct gcataacggc ttgcgcggcg gcactgccga
      361 gcgtgttggc catgtattgc caacgttgcg ccaaagcggg attgtcagga atgcggaaat
      421 cttcgcgcag ttcgtccaca aggtcgggac ggttgcagac gaggacgatg tcgcaacctg
      481 cctcaaagga aatgcgggcg cgttctttga tgccgcctgc cccgcacgcg ccctccatag
      541 tcaaatcatc cgagaaaatt acgcctttaa acccgatgtc gcggcgcaaa atttgtttga
      601 gccagatttc ggaaaaccct gcgggctttg tgtccacttg tggataaacg acgtgggcgg
      661 gcataaccgc cgccatacct tcgcggctca taatgtggaa gggggcgagg tcggcggttt
      721 caagttcgga caggctgcgc ccgtcttcgg gcagcaccaa atggctgtct ccttcgacaa
      781 atccgtgtcc gggaaaatgt ttgccgcatg atttcatgcc gcctttttcc aaaccttttt
      841 gaagggcgag ggcaaggc
//
LOCUS       DAWHSK010000368          841 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.368, whole genome shotgun sequence.
ACCESSION   DAWHSK010000368 DAWHSK010000000
VERSION     DAWHSK010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..841
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.368"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..821
                     /locus_tag="WIC03_002131"
     CDS             <1..821
                     /locus_tag="WIC03_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEZ3884576.1"
                     /translation="KFEIAEETIKLMRQMVENGEADALVAERVWQEFAKGLMEKNPRK
                     MIEVLRECGALKVLLPEVNALFGVPQRADYHPEIDSGIHTLMTLQRAADMGLSLPERY
                     AALLHDLGKAKTPSDILPRHHGHDLAGVEPVREVNQRLRAPKHCAELAELVCRWHIIF
                     HQVGQLKSQTILNVLKKTDAFRRPERFQTALNVCIADTQGRLNREHTPYPQRAHWLAL
                     LEAANQADSGKIAAECWAQGKAHLIAEQIDRARLAQIAPLQKAFRAAQDKTEKH"
BASE COUNT          217 a          261 c          223 g          140 t
ORIGIN      
        1 acaagtttga aatcgccgaa gaaaccataa agctgatgcg gcagatggtg gaaaacggcg
       61 aagcggacgc attggttgcc gaacgcgtct ggcaggagtt tgcgaaaggt ttgatggaaa
      121 aaaatccgcg caaaatgatt gaagtgttgc gcgaatgcgg cgcgctcaaa gtcttgctgc
      181 ccgaagtcaa tgccctcttc ggcgtgccgc aacgcgccga ctaccatccc gaaatcgaca
      241 gcggcatcca taccctgatg acgctgcaac gcgccgccga tatgggtttg agcctgcccg
      301 aacgctatgc cgccctgctg cacgacttgg gcaaagccaa aacaccgtcc gacatcctgc
      361 cgcgccacca cggacacgac ctcgccggtg tcgaacccgt gcgcgaagtc aatcagcggc
      421 tgcgtgcgcc gaaacattgc gccgagcttg ccgaattggt ttgccgttgg cacattattt
      481 tccaccaagt cggacagctt aaaagccaaa ccattctgaa cgtattgaaa aaaaccgatg
      541 ccttccgccg tcccgaacgc tttcagacgg cattgaacgt ctgcattgcc gacacgcaag
      601 gccgtctgaa ccgcgaacac acgccctacc cgcaacgcgc acactggctc gccctgctcg
      661 aagccgccaa tcaggcggat tcgggcaaaa tcgccgccga atgctgggcg cagggaaaag
      721 cgcaccttat cgccgaacaa atcgaccggg cgcggctggc gcaaatcgcc ccgctgcaaa
      781 aagcgtttcg agcggcgcaa gacaaaacag aaaaacatta aaacgtccaa tgcagccact
      841 t
//
LOCUS       DAWHSK010000369          822 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.369, whole genome shotgun sequence.
ACCESSION   DAWHSK010000369 DAWHSK010000000
VERSION     DAWHSK010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..822
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.369"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(94..378)
                     /locus_tag="WIC03_002132"
     CDS             complement(94..378)
                     /locus_tag="WIC03_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884577.1"
                     /translation="MKLALKRSGKYNVYDPLALDLDGDGIETVAAKGFAGALFDHRSQ
                     GIRTATGWIAAYDGFPVRKLNSNGGIISTTDTIFQSLHTWLDHQPNDTCH"
     gene            complement(375..668)
                     /locus_tag="WIC03_002133"
     CDS             complement(375..668)
                     /locus_tag="WIC03_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-regulated frpA domain protein"
                     /protein_id="HEZ3884578.1"
                     /translation="MNIDDFTPSKIANFFADPDTYSNVLEEVSRFIYSLVPDDANPWK
                     GGEDYIGRGISEWGELLEKWYKQDFLPYLEKNGTNFRNLKIGCLNSLNGQESG"
BASE COUNT          230 a          177 c          144 g          271 t
ORIGIN      
        1 atatcctacc tagcccgaac cttcttataa tttgtactat gtgatcattc atttcaatat
       61 ccatttaagc tttcaattaa ttttctatat ttttcaatgg catgtatcat ttggttgatg
      121 atcaagccat gtatgcaaag attggaatat ggtatctgtc gtgctaataa tgcccccgtt
      181 actgtttaat ttgcgcacag gaaaaccgtc atatgcagca atccagcccg tggcggtgcg
      241 gatgccttgg ctgcggtggt cgaacaatgc acctgcaaag cctttggcgg cgacagtttc
      301 tatgccgtca ccgtccaaat ctagggcaag ggggtcgtaa acgttatatt tgcctgaacg
      361 tttgagagct aatttcaacc actctcttgc ccattcaggg aattcaggca gccaatcttc
      421 aaatttcgga aattggtccc attcttttca agataaggga gaaaatcttg tttataccat
      481 ttttccagta actctcccca ttcacttatc cctcgtccaa tataatcttc gccccctttc
      541 caagggtttg catcatcagg aactaaggaa tatataaacc tagatacttc ttctaataca
      601 ttgctgtatg tatcaggatc cgcaaaaaag tttgctatct tacttggtgt aaaatcatct
      661 atattcatta tagattcgcc aagcctctcg tttccataat ccttatttaa ttcaattcat
      721 gctgtaaaac agtagaggat tttagcattt ttctcgtata tctcgagata catttcttaa
      781 atttaaaaaa atgttatcga gggatgcaag taaagaagca gt
//
LOCUS       DAWHSK010000370          805 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.370, whole genome shotgun sequence.
ACCESSION   DAWHSK010000370 DAWHSK010000000
VERSION     DAWHSK010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..805
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.370"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            44..>805
                     /locus_tag="WIC03_002134"
     CDS             44..>805
                     /locus_tag="WIC03_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEZ3884579.1"
                     /translation="MYTRIMEISPWTLRSAKLEKEHKRLQESLTSLGNGYMGMRGSFE
                     ETYSADSHLGTYIAGVWFPDKTRVGWWKNGYPKYFGKAINALNFSKVKIFVDGQEVDL
                     AKNDVAGFSVELDMQHGVLRRSFTVFGVRFNVCKFLSVAQKELAVIRWEAVSVDGKTH
                     QVRIDSIIDADVKNEDSNYEEKFWQVLDKGVSDSLSYIAAQTVANPFGVEQFIVNAEQ
                     TFAGSFKALGGRQTDWQVSNSFEAEVGGTPETFEKR"
BASE COUNT          217 a          221 c          197 g          170 t
ORIGIN      
        1 atttcaccat tccctcaaat caatccaaac aggagcttca taaatgtaca caagaattat
       61 ggaaatcagc ccttggacgc tgcgttcggc aaagctggaa aaagaacaca aacggctgca
      121 agagagcctg accagtttgg gcaacggcta tatgggtatg cgcggcagct ttgaggaaac
      181 ctattccgcc gacagccact tgggtaccta catcgccggt gtgtggtttc ccgacaaaac
      241 ccgcgtcggc tggtggaaaa acggctaccc caaatatttc ggtaaagcca tcaacgcgct
      301 caatttcagc aaagtcaaaa tctttgtcga cgggcaggaa gtggacttgg cgaaaaacga
      361 tgttgccggc ttctccgtcg aacttgatat gcagcacggc gtgttgcgcc gctcgttcac
      421 cgtattcggt gtgcgtttca atgtgtgcaa attcctgtcc gtcgcgcaaa aagagctggc
      481 ggttatccgc tgggaagccg tatccgttga cggcaaaacc caccaagtcc gcatcgattc
      541 catcatcgat gccgacgtga aaaacgaaga ctccaactac gaagaaaaat tctggcaggt
      601 attggacaaa ggcgtttcag acagtctctc ctacattgcc gcccaaaccg ttgccaaccc
      661 tttcggcgtg gaacaattta tcgtcaacgc cgagcaaacc ttcgccggca gctttaaagc
      721 cctcggcggc agacaaaccg actggcaggt ctccaattct tttgaagccg aagtcggcgg
      781 cacacccgaa acctttgaaa aacgg
//
LOCUS       DAWHSK010000371          796 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.371, whole genome shotgun sequence.
ACCESSION   DAWHSK010000371 DAWHSK010000000
VERSION     DAWHSK010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..796
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.371"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..>796
                     /locus_tag="WIC03_002135"
     CDS             <1..>796
                     /locus_tag="WIC03_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HEZ3884580.1"
                     /translation="IRASLGQVNAYLAENQSRYDTWKEGGIGRSILHGAAGGLTTGSL
                     GGILAGSGTSLAAPYLDKAAENLGPAGKAAVNALGGAAIGYATGGSGGAVVGANVDWN
                     NRQLHPSEYKLAEQYADIVASRLNISKEEALGRIIRHMQRTVDYATAKADNFRTDDAI
                     ISILGANNLPKQDQHYTNPNYNKQYIQNYVRDFNLANKYNYFGKTPTQSRDYKVGVVT
                     DIGKAALNSGIGIFESAANLATGEIDPNSWAYVHMGRIGYTHPEYGN"
BASE COUNT          239 a          172 c          204 g          181 t
ORIGIN      
        1 catccgcgca tccctcggcc aagtaaacgc ctaccttgcc gaaaaccaaa gccgctacga
       61 cacctggaaa gaaggcggca taggcaggag catactgcac ggggcggcag gcggactgac
      121 gaccggcagt ctcggcggca tactggccgg cagcggcact tcccttgctg caccgtattt
      181 ggacaaagcg gcggaaaacc tcggtccggc gggcaaagcg gcggtcaacg cactgggcgg
      241 tgcggccatc ggctatgcaa ctggtggtag tggtggtgct gtggtgggtg cgaatgtaga
      301 ttggaacaat aggcagctgc atccgagtga atataaatta gcagaacaat atgcagatat
      361 agtggcttct cgtctaaaca tttcaaaaga agaagcactt gggcgaatta ttcggcatat
      421 gcaacgaacc gtagattacg caacggctaa agcggataat ttccgtactg atgacgccat
      481 tatttcaatt ttaggagcaa acaatttacc caagcaagat caacattaca ctaatccgaa
      541 ctacaacaaa caatacattc aaaattatgt tcgtgatttc aatttagcca ataaatacaa
      601 ttattttggg aaaactccta ctcaatcaag ggattataaa gttggagttg ttacagatat
      661 tggtaaggct gcattaaata gcggtatagg tatatttgaa tcggctgcga atttagcgac
      721 aggggagata gatccgaact catgggctta tgtacacatg gggcgtatag gttatacaca
      781 tcctgaatat ggaaat
//
LOCUS       DAWHSK010000372          793 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.372, whole genome shotgun sequence.
ACCESSION   DAWHSK010000372 DAWHSK010000000
VERSION     DAWHSK010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..793
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.372"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(604..>793)
                     /locus_tag="WIC03_002136"
     CDS             complement(604..>793)
                     /locus_tag="WIC03_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884581.1"
                     /translation="TIMRPFDGVKYKDTLTAGFSSPHHHLTADSYLQKRREPDRWYSR
                     IRRAVREAATSRAPSHDE"
BASE COUNT          143 a          154 c          283 g          213 t
ORIGIN      
        1 ggcaggaaaa agacccgcgc catatcgggg aatgaaccga cgcaaaggaa atgcggtttg
       61 aatatgcgtc cgggcggtgt tttactgtgt gtgccgtatg gcggggcagg gtgtctgatt
      121 tcgtgatgac cgtgttggtg gaatcgaagc ctgccgttcc ggtaagggtt gcccgggagg
      181 gcaaggccgg ctttgtatgc ccggttaaga gggaaggatg agactgtgca tttttgattt
      241 taattggcta tatgccgtct gaaaatcggt ttgaaggcgg tacttttatg ctgcctttca
      301 tacaggggaa tgggaacgtt gcggctgcgc ggaaaccgtg gtgcttgaac tttctactta
      361 ctgcgtttcg ccgtcggcgg gttcggtggt ggattttgac ctgaaaaaaa ggggggggtg
      421 ttgggcaaga tgtttttagg ggaacgccgg attgcggtgg ggtttgcctt tattttgcag
      481 aaggctttaa aaaccgtgtg gtcgggtaca tcggctgtac cgtgcttgtt tgtgcaagat
      541 cggggcaggg tgcgatgggc aagtttgccc gggatgccgt ctgaagcgtt cagacggcat
      601 tttttattcg tcgtggcttg gtgcgcggga tgtggcggct tcccggacgg cacggcggat
      661 gcggctgtac catcggtcgg gttctcgccg cttttggagg taactgtcgg cggtcaggtg
      721 gtggtgcggg cttgaaaaac cggcggttag ggtgtcttta tatttcaccc cgtcaaaagg
      781 gcgcattata gtg
//
LOCUS       DAWHSK010000373          778 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.373, whole genome shotgun sequence.
ACCESSION   DAWHSK010000373 DAWHSK010000000
VERSION     DAWHSK010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..778
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.373"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(175..333)
                     /locus_tag="WIC03_002137"
                     /pseudo
     CDS             complement(175..333)
                     /locus_tag="WIC03_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249760.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            489..>778
                     /locus_tag="WIC03_002138"
     CDS             489..>778
                     /locus_tag="WIC03_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR domain-containing protein"
                     /protein_id="HEZ3884582.1"
                     /translation="MNKTLYRVIFNRKRGAVVAVAETTKREGKSCADSDSGSAHVKSV
                     PFGTTHAPVCRSNIFSFSLLGFSLCLAVGTANIAFADGIIADKAAPKTQQAT"
BASE COUNT          245 a          153 c          147 g          233 t
ORIGIN      
        1 cgtccaaaat gctctttggg aatgctattg gattatagac cttacaacag aaaaaaaggt
       61 cggaaagtag aaagactgct ttttaaattc aaacggccaa gcaagtaaag acttggccaa
      121 caaatgggac aaaatccgac tgttttaaat tgccagtcaa atactggaaa acttgcgtaa
      181 atggctaaat tcactgacat caagtacatc ataactacga aaggtatccg tatacacaat
      241 gccatcagac ttaactttct ctcggataat cggcaataat gttgctgatt gcgcattagg
      301 gacaacgacg gtataaacct tgccatttcg ttcgaatggg atttttaacc gttatctagt
      361 aaagtcccta atttaataaa caaatggcag taaaatggcg gtaattgact cgccaaaatc
      421 cttgatctag aatggtcaga ctatccaatt ttttctaata actctataaa aatcaaataa
      481 ctgaaatcat gaataaaact ctctatcgtg taattttcaa ccgcaaacgt ggggctgtgg
      541 tagccgttgc tgaaactacc aagcgcgaag gtaaaagctg tgccgatagt gattcaggca
      601 gcgctcatgt gaaatctgtt ccttttggta ctactcatgc acctgtttgt cgttcaaata
      661 tcttttcttt ttctttattg ggcttttctt tatgtttggc tgtaggtacg gccaatattg
      721 cttttgctga tggcattatt gctgataaag ctgctcctaa aactcaacaa gccacgat
//
LOCUS       DAWHSK010000374          740 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.374, whole genome shotgun sequence.
ACCESSION   DAWHSK010000374 DAWHSK010000000
VERSION     DAWHSK010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..740
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.374"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..217
                     /locus_tag="WIC03_002139"
     CDS             <1..217
                     /locus_tag="WIC03_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884583.1"
                     /translation="TDDLMLITSGGVLIRTKVEQIRETGRAAAGVKLINLDEGETLVS
                     LERVAEDESELSDASVISNVTEPEVEN"
     gene            374..740
                     /locus_tag="WIC03_002140"
                     /pseudo
     CDS             374..740
                     /locus_tag="WIC03_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249760.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          236 a          155 c          161 g          188 t
ORIGIN      
        1 aaccgacgat ttgatgctga ttaccagcgg cggcgtactt atccgcacca aagtcgaaca
       61 aatccgcgaa accggccgcg ccgcagcagg cgtgaaactg attaacttgg acgaaggcga
      121 aaccttggta tcgctggaac gtgttgccga agacgaatcc gaactctccg acgcttctgt
      181 aatttccaat gtaaccgaac cggaagtcga gaactgaaaa tcatctcccg atgccgtctg
      241 aagattcaga cggcatttat tttatccctc atccgtcatc cagcttctca caatatagca
      301 gattatagtc aattaaaaac aaggggctgt cctagataac tagggaaatt caaattaagt
      361 tagagttgcc cctatgagaa aaagtcgtct aagccggtat aaacaaataa actcattgaa
      421 ctgtttgtcg caggtgtaac tgcaagaacg acagcagagt tagtaggcgt taataaaagt
      481 accgcagcct attattttca tcgtttacga ttacttattt atcaaaacag tccgcatttg
      541 gaaatgtttg atggcgaagt agaagcagat gaaagttatt ttgctgaacg acaaaaccat
      601 attaatggaa ttgagaactt ttggaaccgg gcaaaacgtc atttacgcaa gtttgacggc
      661 attcccaaag cgcattttga gctgtattta aaggggtacg aacggcgttt taacaacagt
      721 gagataaaag ttcaaatttc
//
LOCUS       DAWHSK010000375          738 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.375, whole genome shotgun sequence.
ACCESSION   DAWHSK010000375 DAWHSK010000000
VERSION     DAWHSK010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..738
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.375"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(17..412)
                     /locus_tag="WIC03_002141"
     CDS             complement(17..412)
                     /locus_tag="WIC03_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884584.1"
                     /translation="MMYSFEANANAVKISETVSVDTGQGAKIHKFVPKNSKTYSSDLI
                     KTVDLTHIPTGAKARINAKITASISRAGVLAGGGKLARLGAKFSTRAVPYVGTALLAH
                     DVYETFKEDIQARGYQYDTETDKFAKVSG"
     gene            complement(396..713)
                     /locus_tag="WIC03_002142"
     CDS             complement(396..713)
                     /locus_tag="WIC03_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1132 family protein"
                     /protein_id="HEZ3884585.1"
                     /translation="MNKPFITQAQLALYKYQPSSKYFGQSMAVIAQSEFVEFAKINKS
                     ENVIDCFSFFWNRRIKHDIWLISFSDNSEMVIKESLKDGHKIYKFEFCEIVDNCNFDD
                     VFV"
BASE COUNT          197 a          152 c          145 g          244 t
ORIGIN      
        1 gcggcaacag gcgcacttag cctgagacct ttgcaaattt gtcggtttcg gtgtcgtatt
       61 ggtagcctcg tgcctgtatg tcttctttga aagtttcgta tacgtcgtgg gctaaaaggg
      121 ctgttccgac atagggaacc gcccttgtgc tgaatttcgc gcctaagcgg gcaagtttgc
      181 cgccccccgc caatacgccg gcgcgggaaa tgctggcggt tattttggcg ttgattcggg
      241 cttttgcgcc cgtagggatg tgtgttaaat ctaccgtttt tattaaatca gatgaataag
      301 ttttactatt tttaggtaca aacttatgaa ttttcgcacc ttgtccggta tcaactgaaa
      361 cagtttcaga tatttttact gcatttgcat tcgcttcaaa cgaatacatc atcaaaattg
      421 caattatcga caatttcgca aaattcaaat ttgtatattt tatgaccatc tttcagggat
      481 tctttaatta ccatttctga attatcagaa aatgagatta gccaaatatc atgtttaatt
      541 cttctattcc agaaaaaaga gaaacaatca ataacatttt cagacttatt aatcttcgca
      601 aattcaacaa attcagattg cgctataacc gccatcgatt gcccaaaata cttgcttgac
      661 ggctgatatt tataaagtgc caactgcgcc tgagtgataa acggcttgtt catggttctg
      721 cctttcaatg attgtttt
//
LOCUS       DAWHSK010000376          730 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.376, whole genome shotgun sequence.
ACCESSION   DAWHSK010000376 DAWHSK010000000
VERSION     DAWHSK010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..730
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.376"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..363)
                     /locus_tag="WIC03_002143"
     CDS             complement(<1..363)
                     /locus_tag="WIC03_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEZ3884586.1"
                     /translation="MIKFKNVHKHFKDLHVINGVNLEIKKGEVVVVCGPSGSGKSTLI
                     RTVNQLESIESGEIWVDGVNVADPKTDLNKIREEVGFVFQSFNLYPHLTVLENITLAP
                     MKVKGQNAEQAEKKAMELL"
     gene            complement(536..>730)
                     /locus_tag="WIC03_002144"
     CDS             complement(536..>730)
                     /locus_tag="WIC03_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase"
                     /protein_id="HEZ3884587.1"
                     /translation="DFEYFAKLVKLAFHQRRKTIRNNLKELAGDDDLQAVDINPQDRA
                     EHIAPEKYVALSNYLAGKAV"
BASE COUNT          144 a          172 c          192 g          222 t
ORIGIN      
        1 caaaagttcc atcgcttttt tctctgcctg ttccgcattt tgacctttaa ccttcatcgg
       61 tgcgagggta atgttttcca acacggtcag gtgcgggtag aggttgaaac tttgaaacac
      121 aaagccgact tcttcgcgga ttttgttcaa atcggttttt gggtcggcaa cgttgacacc
      181 gtccacccaa atctcgccgc tttcgatgct ttcaagctgg ttgacggtgc ggatgagtgt
      241 ggatttgccg ctgcccgaag gtccgcagac gacgaccact tcgccttttt tgatttccaa
      301 gttcacgccg ttgatgacgt gcaggtcttt gaaatgtttg tgtacgtttt tgaatttgat
      361 catgtctttt ctttcagacg gcatttgccg ttgcaggttg tcgttacggg agctccatat
      421 gatgaagcgt gtagcgtctg ccgtcaaaaa aacggtcgtt cggattggtc aggcaggctg
      481 caagcggcag tatcaggggt aaaagcaggt atttcgtcat cggcttactc ccttttcaga
      541 cggccttgcc cgctagataa ttgctcaacg ccacatattt ctccggcgcg atatgttcgg
      601 cacggtcttg cggattgatg tcgactgcct gcaaatcatc gtcgcctgca agctctttca
      661 gattgttgcg tatggttttg cggcgttggt ggaaggcgag tttcacgagt ttggcgaaat
      721 actcgaaatc
//
LOCUS       DAWHSK010000377          729 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.377, whole genome shotgun sequence.
ACCESSION   DAWHSK010000377 DAWHSK010000000
VERSION     DAWHSK010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.377"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            494..>729
                     /locus_tag="WIC03_002145"
     CDS             494..>729
                     /locus_tag="WIC03_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ3884588.1"
                     /translation="MNTLQKGFTLIELMIVIAIVGILAAVALPAYQDYTARAQVSEAI
                     LLAEGQKSAVTEYYLNHGEWPANNSSAGVATSTD"
BASE COUNT          244 a          202 c          118 g          165 t
ORIGIN      
        1 ggaaacctgc cgcgatgatt caactgccgt ttgcacagga acaccacgcg ccgatttcaa
       61 tcactttcca agaaaacgga aatttttaaa aattaaattt aaaatttccc acccaaccca
      121 ccctatgcta ccgcgcaaat tcaaaaatct caaattccga cccaatcaac acacccgatg
      181 ccccatgcca atagagatac cccacgccaa taaaaaagtg ccgaaaatcg gcactaaaac
      241 tgacaatttt cggcactgct gacaaaaaac ggtaatcccc tgcttcctca aaccacgccc
      301 aaacgaaaga aaatgtaaaa taaaaacaac catataaaga taaacgcata aattttcacc
      361 tcaaaacaca aacttgatac caatcttgct ttataataca caggtatcgc aacaaaatac
      421 cgatggtcaa atacattgca taatgccgat ggcgtaagct tgaggcattt cctttccaat
      481 taggagtaat tttatgaaca cccttcaaaa aggttttacc cttatcgagc tgatgattgt
      541 gattgccatc gtcggcattt tggcggcagt cgcccttcct gcttatcaag actacacagc
      601 ccgcgcacaa gtttccgaag ccattctttt ggctgaaggt caaaaatcag ccgtcaccga
      661 gtattacctg aatcacggcg aatggcccgc caacaacagt tctgccggcg tggcaacctc
      721 caccgacat
//
LOCUS       DAWHSK010000378          715 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.378, whole genome shotgun sequence.
ACCESSION   DAWHSK010000378 DAWHSK010000000
VERSION     DAWHSK010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..715
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.378"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..341)
                     /locus_tag="WIC03_002146"
     CDS             complement(<1..341)
                     /locus_tag="WIC03_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884589.1"
                     /translation="MHTQRKNFSDSISLHDCYCTEIKVEGRNVCFNFEDGFVVPDNNP
                     NNQAGKHLKTDFSRVVLTHKNENAEDDYLFDIFEDIVFLGKRLCTVRKFLDFSELLEM
                     VNTQGYFLEFL"
     gene            439..>715
                     /locus_tag="WIC03_002147"
     CDS             439..>715
                     /locus_tag="WIC03_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEZ3884590.1"
                     /translation="MSTFFRQTAQAMIAKHIDRFPLLKLEQVIDWQPIEHYLNRQKTR
                     YLRDHRGRPAYPLLSMFKAVLLGQWHSLSDPELEHSLITRIDFNLFCR"
BASE COUNT          218 a          191 c          116 g          190 t
ORIGIN      
        1 tacagaaact caagaaaata accttgtgta ttgaccatct caagcaattc agaaaaatca
       61 agaaattttc tgaccgtaca caaacgtttc cctaaaaaaa cgatgtcttc aaaaatatcg
      121 aacaaataat catcttcggc gttttcattt ttatgggtta aaacaacacg ggaaaaatct
      181 gttttcagat gcttgcccgc ttgattgttc ggattattgt ccggaacgac aaaaccgtcc
      241 tcaaaattaa agcagacgtt gcgtccttct acctttatct ctgtgcaata acaatcatgt
      301 agagaaatgc tatccgaaaa attttttctt tgtgtatgca aaaaaagttt tcattcaagt
      361 acccatatct aacacgagac ctttgcaaaa ttccccaaaa tcccctaaat tcccaccaag
      421 acatttaggg gattttccat gagcaccttc ttccggcaaa ccgcacaagc catgattgcc
      481 aaacacatcg accgtttccc actattgaag ttggagcagg tgattgattg gcagccgatc
      541 gaacactacc tgaaccgtca aaaaacccgt taccttagag accaccgcgg ccgtcctgcc
      601 tatcccctgc tgtccatgtt caaagccgtc ctgctcggac aatggcacag cctctccgat
      661 cccgaactcg aacacagcct cattacccgc atcgacttca acctgttttg ccgtt
//
LOCUS       DAWHSK010000379          704 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.379, whole genome shotgun sequence.
ACCESSION   DAWHSK010000379 DAWHSK010000000
VERSION     DAWHSK010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.379"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..>704
                     /locus_tag="WIC03_002148"
     CDS             <1..>704
                     /locus_tag="WIC03_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEZ3884591.1"
                     /translation="TNPLPSPLPQEREQSTAAISDSLKTKSSLYPIENSLHGQIQQQP
                     APSPVGEGWGEGKAVAAQSAIAVITESDLYQYVARSRIHNRRKKHAAVSDDLLRDLAE
                     INIGDPVVHEEHGIGRYMGLITMNLGGETNEMMLLEYAGEAQLYVPVSQLHLISRYSG
                     QAHENIALHKLGSGAWNKAKRKAAEKARDTAAELLNLYARRAAQSGHKFEINELDYQA
                     FADGFGYEETEDQAAA"
BASE COUNT          183 a          222 c          182 g          117 t
ORIGIN      
        1 aacaaaccct ctccctagcc ctctcccaca ggagagggaa caaagtaccg ccgccatttc
       61 agacagtctg aaaaccaaaa gcagcctgta ccccatcgaa aatagtctgc acgggcaaat
      121 ccaacagcaa cctgccccct cccccgtggg ggagggttgg ggagagggca aagcagttgc
      181 cgctcaaagt gccatcgccg tcatcaccga atccgatctc taccaatacg tcgcccgttc
      241 gcgcatccac aaccgccgca agaaacacgc cgccgtttca gacgacctgt tgcgcgacct
      301 tgccgaaatc aacatcggcg accccgtcgt gcacgaggaa cacggcatcg gccgatatat
      361 gggcttgata acaatgaact tgggcggcga aaccaacgaa atgatgttgc tcgaatatgc
      421 gggcgaagcg cagctttatg tgcctgtttc gcaactgcat ttaatcagcc gctactccgg
      481 tcaggcgcat gaaaacattg ccctgcacaa gctcggcagt ggcgcgtgga acaaggcgaa
      541 gcgcaaagcc gccgaaaagg cgcgcgatac cgccgccgag ttgcttaacc tttacgcccg
      601 acgtgccgcc caatcgggac acaagtttga aatcaacgag ttggattatc aggcgtttgc
      661 cgacggcttc ggctacgaag aaaccgaaga ccaggccgcc gcca
//
LOCUS       DAWHSK010000380          686 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.380, whole genome shotgun sequence.
ACCESSION   DAWHSK010000380 DAWHSK010000000
VERSION     DAWHSK010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.380"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            14..334
                     /locus_tag="WIC03_002149"
     CDS             14..334
                     /locus_tag="WIC03_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEZ3884592.1"
                     /translation="MGEKIKDGKKTVLSCEEEQKICNTFTNKQAVEDFSVVIGYDEIK
                     AKNHSLSAGQYFEVKIDYVDISADEFAQKMAGFSADLDKLFAESAELEKEIKDRLAML
                     KFNS"
     gene            345..482
                     /locus_tag="WIC03_002150"
     CDS             345..482
                     /locus_tag="WIC03_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEZ3884593.1"
                     /translation="MKPVKLKMPSESLSDGILFGLENTYAVLSLKERIQKTKATGIYR
                     K"
BASE COUNT          241 a          134 c          147 g          164 t
ORIGIN      
        1 cgacgcatcg ggcttgggcg aaaaaattaa agacggcaaa aaaaccgtac tttcctgcga
       61 agaagaacaa aaaatctgca atactttcac gaacaaacag gcagtggaag atttcagtgt
      121 ggtaatcggc tacgatgaaa tcaaagcgaa gaatcacagc ttgtcggcgg ggcagtattt
      181 cgaggtaaag attgattatg tggatatttc cgccgacgaa tttgcgcaaa aaatggcggg
      241 attttcagcg gatttggata aacttttcgc cgaatctgcc gaattggaaa aagagattaa
      301 ggatagattg gcgatgttga aattcaattc ataaataccg atgaatgaaa ccggtcaaac
      361 ttaaaatgcc gtctgaaagc ctttcagacg gcattttgtt tggtttggaa aatacttatg
      421 ccgttctttc cctgaaagag agaatccaaa aaaccaaagc cacaggaatt tatcggaaat
      481 gacaaaaacc cgacgaaccg gattcccgcc tgcgcgggaa tgacgaatta gaagttaccc
      541 gaaatttgca aaaacaaaat ccaacccaac agaccgggtt ttcgtttgca cggaaataat
      601 gcaataaata aagcaaatat aaagtatttg aatttactat attatttttc cgcttcttca
      661 aagccgacga cttccaaacc gaagcc
//
LOCUS       DAWHSK010000381          684 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.381, whole genome shotgun sequence.
ACCESSION   DAWHSK010000381 DAWHSK010000000
VERSION     DAWHSK010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.381"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..575)
                     /locus_tag="WIC03_002151"
     CDS             complement(<1..575)
                     /locus_tag="WIC03_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ3884594.1"
                     /translation="MKFLDREASIAKPGFNRWLVPPAALAVHLAIGQIYAYSVFNAPL
                     TKLIGITESAAGDWKLTTVGWIFSIALAMLGASAALFGTWMERVGPRKAIFAAACCFS
                     LGFFVSAFGVSTHNLFLLYLGNGVIGGVGLGLGYIGPVSTLMKWFPDKPGMATGLAIM
                     GFGGGAMLASPLSVSLMNAFSNAASVGVVET"
BASE COUNT          180 a          188 c          178 g          138 t
ORIGIN      
        1 aaggtttcaa caaccccgac tgaagcggcg tttgaaaagg cgttcatcag tgatacggaa
       61 agcggagagg ccagcattgc gccgccgccg aaacccataa tcgccaaacc ggtcgccata
      121 cccggcttgt cgggaaacca tttcatcagt gtggaaaccg gcccgatgta gcctaagcct
      181 aagcctacgc caccgatgac gccgttgccc aaataaagca ggaagaggtt gtgcgtactc
      241 acgccgaatg cggatacgaa gaagcccagg ctgaaacagc aggcggcggc aaatatggct
      301 ttgcgcggcc ctacccgttc catccatgtg ccgaacaggg cggccgacgc gcccagcatc
      361 gcgagtgcga tactgaaaat ccaacctacg gtcgtcagct tccaatctcc ggccgccgat
      421 tcggttatgc cgataagttt ggtcagcggc gcgttgaata cggaataggc gtaaatctgc
      481 ccgatggcaa ggtgtaccgc caatgctgcg ggcggtacga gccaacggtt gaaacccggc
      541 ttggcaatgc ttgcctcacg gtctaaaaac ttcataacat cctctttctg tcagttgaaa
      601 aataaaattt catttgctca atggaaactt attgaaaatt ataaaaaaat atcgggtcgg
      661 gtttttatcc gccccaagat gcgc
//
LOCUS       DAWHSK010000382          683 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.382, whole genome shotgun sequence.
ACCESSION   DAWHSK010000382 DAWHSK010000000
VERSION     DAWHSK010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.382"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..204
                     /locus_tag="WIC03_002152"
     CDS             <1..204
                     /locus_tag="WIC03_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HEZ3884595.1"
                     /translation="LSSTGARCNHIDHVTSQLPYALTVAAAAASGYLALGLTKSALLG
                     FGTTGIVLAVLIFLLKDKKRANA"
     gene            261..>683
                     /locus_tag="WIC03_002153"
     CDS             261..>683
                     /locus_tag="WIC03_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEZ3884596.1"
                     /translation="MTVPHIPRGPVMADIAAFRLTGEERQRLLDPAIGGIILFRRNFQ
                     NIEQLKELTAEIKSLRTPELIIAVDHEGGRVQRFIEGFTHLPAMNVLGQIWDKDGASA
                     AETAAGQVGRVLATELSACGIDLSFTPVLDLDWGNCPVI"
BASE COUNT          145 a          232 c          167 g          139 t
ORIGIN      
        1 ctgtcgtcca ccggcgcgcg ctgcaaccac atcgaccacg ttacctcgca actgccttac
       61 gccttaaccg ttgccgccgc cgccgcatcg ggctacctcg cattgggtct gacaaaatcc
      121 gcgctgttgg gctttggcac gacaggcatt gtattggcgg tgctgatttt tctgttgaaa
      181 gataaaaaac gcgccaacgc ctgacccctt tccctgttca gacggcatat gccgtctgaa
      241 gctttttgga gcaaaccgca atgaccgtcc cccatattcc gcgcggcccc gtcatggcag
      301 acattgccgc cttccgcctg accggagaag aaaggcaacg cctgctcgac cccgcaatcg
      361 gcggcatcat cctcttccgc cgcaacttcc aaaacatcga acagctcaaa gaactcaccg
      421 ccgaaatcaa atccctgcgc acacccgaac tcattattgc cgtcgatcac gaaggcggca
      481 gggtgcaacg tttcattgaa ggcttcaccc acctgcccgc aatgaacgtt ttagggcaaa
      541 tttgggacaa agacggcgcg tccgccgccg aaaccgccgc cggacaagtc ggccgggttt
      601 tggcaaccga gctttccgcc tgcggcatcg acttgtcctt cacgcccgtc ttagatttgg
      661 actggggaaa ctgccccgtc atc
//
LOCUS       DAWHSK010000383          681 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.383, whole genome shotgun sequence.
ACCESSION   DAWHSK010000383 DAWHSK010000000
VERSION     DAWHSK010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..681
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.383"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(80..565)
                     /gene="fxsA"
                     /locus_tag="WIC03_002154"
     CDS             complement(80..565)
                     /gene="fxsA"
                     /locus_tag="WIC03_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009349087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HEZ3884597.1"
                     /translation="MRFFGIGFLVLLFLEIMSIVWVADWLGGGWTLFLMAAGFAAGVL
                     MLRHTGLSGLLLAGAAMRNGGRVSVYQMLWPIRYTVAAVCLMSPGFVSSVLAVLLLLP
                     FKGGAVLQAGGAENFFNMNQSGRKEGFSRDDDIIEGEYTVEEPYGGNRSRNAIEHKKD
                     E"
BASE COUNT          200 a          211 c          122 g          148 t
ORIGIN      
        1 gtactggttt ttgttaatct actatatttt caaaccggaa aaatgccgtc tgaaccttca
       61 gacggcattc cattcatatt tattcgtctt ttttgtgttc gatggcgttt cgggaacgat
      121 tgccgccgta aggctcttca accgtatatt ccccctcgat aatatcgtca tcgcgggaaa
      181 agccctcttt tctgcccgat tggttcatgt tgaaaaaatt ttccgcacct cctgcctgca
      241 acactgcacc tcccttaaac ggcagcagca gcaataccgc caacaccgag gatacgaatc
      301 ccggactcat cagacacacc gccgccaccg tataacggat aggccacaac atctgataaa
      361 cggatactct tccgccgttt ctcattgccg cgcccgccaa taaaagaccg gacagccccg
      421 tatgcctgag catcagcaca ccggcggcaa aacctgccgc catcaaaaac aacgtccagc
      481 cgccgcccaa ccaatcggca acccacacaa tcgacataat ctccaaaaac agcagcacca
      541 aaaaaccgat accgaaaaat ctcattgacc gtcatcctta tatttaagta aacagcaaac
      601 cgcccgaaca ggactccaag cgagctgcct gtaaatgatt acaaaaccat gtgcttcaag
      661 ccgaaacaat gtgaaatcac g
//
LOCUS       DAWHSK010000384          681 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.384, whole genome shotgun sequence.
ACCESSION   DAWHSK010000384 DAWHSK010000000
VERSION     DAWHSK010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..681
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.384"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..325)
                     /locus_tag="WIC03_002155"
     CDS             complement(<1..325)
                     /locus_tag="WIC03_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T cell/B cell stimulating protein TspB"
                     /protein_id="HEZ3884598.1"
                     /translation="MYALSEKYNDNGFKAYKVLGEGGGIHTEYNYKFDKSLNLNVLES
                     STGARSLEKVPVKVTASVSRAAVLSGVGKLARLGAKLSTRAVPYVGTALLAHDVYETF
                     KEDIQA"
BASE COUNT          230 a          124 c          102 g          225 t
ORIGIN      
        1 gtgcctgtat gtcttctttg aaagtttcgt atacgtcgtg ggctaaaagg gctgttccga
       61 cataaggaac tgcccttgtg cttaatttcg cgcctaagcg ggcaagtttg ccgactcctg
      121 acaagacggc ggcgcgggaa actgatgcag ttactttaac ggggactttt tcaagagagc
      181 gtgcgcctgt tgaactttct aatacattca aattcaaact tttatcaaat ttgtaattat
      241 attctgtatg aattcctcct ccctcaccta aaactttgta tgccttaaat ccattatcgt
      301 tatatttttc cgaaagtgca tacatcaatt tcccattttt aatttctaaa tctgctgaaa
      361 aagatttagc actcaaaaga aacagaaaag aaattattag aatccgaaac atcaattttt
      421 ccattgccaa taatgaaaaa tgaatcatcc ttgaatttaa tttcaaagca agattcatta
      481 aaattaattc tattaaaaaa attatgacat ttatccattg agaaattttt caatagacca
      541 gtttctttca aataacaata aaacacataa gaaagaggcc tatcaggata agattctgac
      601 aatttatcta aatcagaatt tgatatatag aaaaagtcac gttcattttt attcatggtt
      661 tcaagcctca aatgtctaat t
//
LOCUS       DAWHSK010000385          680 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.385, whole genome shotgun sequence.
ACCESSION   DAWHSK010000385 DAWHSK010000000
VERSION     DAWHSK010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..680
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.385"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(38..604)
                     /locus_tag="WIC03_002156"
     CDS             complement(38..604)
                     /locus_tag="WIC03_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HEZ3884599.1"
                     /translation="MSLYALLLIALGMSMDAFAVALAKGAAVRMPPRKIAATALVFGS
                     VEALTPLAGWVGGFYAKPFISEWDHWAAFVLLGGLGLKMMREGLSGKAEDVRESKRES
                     LWMTVLTAFGTSIDSMIVGVGLAFMEVNIAFAAAIIGMATTVMVAVGLTAGRALGVLF
                     GRRAEFAGGLVLIAIGTWTLLSHLGLIQ"
BASE COUNT          187 a          233 c          136 g          124 t
ORIGIN      
        1 cgtactggtt tttgttaatc cactatattt tccgacatca ttgaatcaaa cccaaatgcg
       61 acaagagcgt ccatgtaccg atggcaatca acaccaaacc tccggcaaat tccgcacgcc
      121 tgccgaacaa tacgcccaaa gcccttcccg ccgtcagccc gaccgccacc atcaccgtcg
      181 tcgccatacc gatgattgcg gcggcaaagg cgatgtttac ctccataaac gccaagccca
      241 ccccgactat catggaatca atactggttc caaaagcagt caaaaccgtc atccataggc
      301 tttcccgttt gctttcgcgc acatcttccg ccttgccgga cagcccttcg cgcatcattt
      361 tcagacccag cccgcccagc aggacgaaag ccgcccaatg gtcccattcg ctgataaacg
      421 gtttggcata aaaaccgcct acccagcctg ccagcggcgt gagtgcttca accgagccga
      481 acaccaaggc tgttgctgca attttgcgcg gaggcatcct gaccgccgca ccctttgcca
      541 atgcgacggc aaacgcatcc atcgacatcc ccagcgcaat caagagcaaa gcgtaaagac
      601 tcataccgca cccatcctca aaaagggcag attatagcaa aacagcaaag cgcgaaaaac
      661 gtcgcacgaa aacccacatc
//
LOCUS       DAWHSK010000386          668 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.386, whole genome shotgun sequence.
ACCESSION   DAWHSK010000386 DAWHSK010000000
VERSION     DAWHSK010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.386"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..>668
                     /gene="purH"
                     /locus_tag="WIC03_002157"
     CDS             <1..>668
                     /gene="purH"
                     /locus_tag="WIC03_002157"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360677.1"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEZ3884600.1"
                     /translation="KMEEHGIGNIDLVCVNLYPFAATIAKPGCTLEDAIENIDIGGPT
                     MVRSAAKNWKHVAIVTDTADFPAIAAEMEANNGALSDKTRFNLSRKAFSHTAQYDGMI
                     SNYLTSLSDDVLSGQPQIGEFPSRFNQSWIKVQDMRYGENPHQRAAFYRDIYPAAGSL
                     AAYKQLQGKELSYNNIADADAAWEAVKSFDAPACVIVKHANPCGVAVAADTLTAYKLA
                     YVTDT"
BASE COUNT          177 a          223 c          152 g          116 t
ORIGIN      
        1 aagatggaag aacacggtat cggcaacatc gaccttgtgt gtgtcaacct ctaccccttc
       61 gccgccacca ttgccaagcc cggctgcacg ctggaagacg cgattgaaaa catcgacatc
      121 ggcggcccga ccatggtgcg ttctgccgcg aaaaactgga aacacgtcgc catcgttacc
      181 gacacagccg atttcccggc catagctgcc gaaatggaag ccaacaacgg cgcattgagc
      241 gacaaaaccc gcttcaacct ctcacgcaaa gcattcagcc ataccgccca atacgacggc
      301 atgatttcca actacctgac ctcgctttca gacgacgtct tgagcggcca gccccaaatc
      361 ggcgaattcc caagtcggtt caaccaaagc tggattaaag tgcaagacat gcgctacggc
      421 gaaaatccgc accaacgcgc cgcgttctac cgcgatattt accccgccgc aggcagcctc
      481 gctgcataca aacaactgca aggcaaagaa ttgtcctaca acaacatcgc cgatgccgat
      541 gccgcatggg aagccgtcaa atcctttgac gcaccagcct gtgtgattgt gaaacacgcc
      601 aatccgtgcg gcgtcgccgt tgcagccgat acattgaccg cctacaaact cgcctacgtc
      661 accgacac
//
LOCUS       DAWHSK010000387          647 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.387, whole genome shotgun sequence.
ACCESSION   DAWHSK010000387 DAWHSK010000000
VERSION     DAWHSK010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..647
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.387"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..627
                     /locus_tag="WIC03_002158"
     CDS             <1..627
                     /locus_tag="WIC03_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEZ3884601.1"
                     /translation="IMLDTADGLTKRQGINAWMVGCGLGTDKAAVGTLAGILAEHTDK
                     PVLLDADALNILSTDAETRNLARGCKNLILTPHPAEAARLLGTTVAQVQADRTAAVRK
                     IGAIFGATVVLKGHKTLVAASDTEIYVNESGNAGLATAGSGDVLGGIIGSLLAQGVPV
                     FEAACAGAWLHGAAADVIKESAGIAAGLLAGEIAPAARWLRNRITKSM"
BASE COUNT          153 a          154 c          220 g          120 t
ORIGIN      
        1 attatgttgg acacggcaga cggtttgacc aagcgtcaag gcataaacgc ttggatggta
       61 ggttgcggcc tgggtacgga taaagcggcg gtcggaacgc ttgccggaat tttggcggaa
      121 cacacggaca agcccgtcct tttggatgcg gacgcgctga acatattatc aaccgatgcc
      181 gaaacccgaa atctggcgcg cgggtgtaaa aacctgattt taacgccaca ccccgccgaa
      241 gccgcgcgcc tgcttggaac gacggttgca caggttcagg cggatcggac ggcggcagtg
      301 aggaagatag gggcaatttt cggcgcaacc gtggttttaa aggggcacaa aacattggtt
      361 gctgcgtccg atacggaaat ctatgtcaac gaaagcggca acgcgggatt ggcaacggcg
      421 ggcagtggcg acgtattggg cggcatcatc ggcagtctgc tcgcacaggg cgtgccggtt
      481 tttgaagccg cctgcgcggg cgcgtggctg cacggcgcgg cggcggatgt cataaaagaa
      541 tcggcaggca ttgcggcagg gctgttggca ggggaaatcg ctccggcggc aaggtggctg
      601 cgcaaccgga taactaaaag tatgtaagaa gatatagtgg attaaca
//
LOCUS       DAWHSK010000388          646 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.388, whole genome shotgun sequence.
ACCESSION   DAWHSK010000388 DAWHSK010000000
VERSION     DAWHSK010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.388"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..>646
                     /locus_tag="WIC03_002159"
     CDS             <1..>646
                     /locus_tag="WIC03_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEZ3884602.1"
                     /translation="APEIIAGKSRQYGFGSDSSFRFERGVDYRLQADAIERATELVLQ
                     ICGGAAGEMVEAQGELPEAKQVGLRLDRLKTVLGVDIPAEQVETILQHLGLQPEKTAE
                     GFRVTAPSFRFDIEIEADLIEEIGRVYGYENIPDDYTSGRLKMLELPETRRPRFAVYN
                     EMAARGYREVVSYAFVDEQWEQDFAANADPIRLQNPLAAQYAVMRSTLIGGLVE"
BASE COUNT          137 a          171 c          197 g          141 t
ORIGIN      
        1 ttgcgcccga aatcatcgcc ggcaaatcgc gccaatacgg tttcggttcg gattcgtcgt
       61 tccgcttcga gcgcggcgtg gattaccgtt tgcaggcgga tgccattgaa cgtgctaccg
      121 aattggtgtt gcagatttgc ggtggtgcgg caggcgagat ggtggaagcg caaggcgaat
      181 tgcctgaagc gaagcaggtt ggattgcgtt tggaccgtct gaaaaccgtg ttgggcgtgg
      241 acattcctgc cgaacaggtg gaaaccattt tgcaacactt gggcctgcag cccgagaaaa
      301 cggcggaagg cttccgcgtt accgcgccga gcttccgttt tgacatcgaa attgaggctg
      361 atttgattga agaaatcgga cgcgtttacg gctatgaaaa catccccgac gattacacgt
      421 caggccgtct gaaaatgctg gaactgcccg aaacacgccg cccgcgtttt gccgtttaca
      481 acgaaatggc ggctcgcggt taccgcgaag tggtcagcta tgccttcgtt gacgagcagt
      541 gggaacaaga ttttgccgcc aacgccgacc ccatccgcct gcaaaacccg ctggcggcgc
      601 agtatgccgt gatgcgttcc acgctcatcg gcggcttggt ggaaat
//
LOCUS       DAWHSK010000389          646 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.389, whole genome shotgun sequence.
ACCESSION   DAWHSK010000389 DAWHSK010000000
VERSION     DAWHSK010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.389"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
BASE COUNT          144 a          164 c          155 g          183 t
ORIGIN      
        1 tttaatttgt ctgtccgcat tgccgctaat tgtaccactt catcgtattt ccttttcggt
       61 tgaaaccccg ccctttaggg cggcaggatc agactttatt tgggaggggg tgtaacccct
      121 tccaaatcag gacgacacat agggaggtgc tttatgtgtc gtcctgtgtg ttgaaatata
      181 tcggacgcgt ttgaacagtt gtgcggcaaa ctgttttagc ggcaggcaag agatgccgtc
      241 tgatgccgtc tgaagcccat tgtccgtcat tcccacacag gcagttgcta cggttgctga
      301 agccccttag ccgtcgcgca ggcggttgct acggttacta aaatcccgtc attcccgcgc
      361 aggcgggaat ccagtccgtt cggtttcagt tgtttctgat aaatcttgca gcgttgggtt
      421 tttagattcc cgcttgcgcg ggaatgacgg tagagcggtt tcagttgctc ccgataaatg
      481 ccgcaatctc aaatcccgtc attccctcaa aaacagaaaa acaaaatcag aaacctaaaa
      541 tcccgtcatt cccgcagctc ttcgtcattc ccgtgaaagc gggaatctag accttttaaa
      601 ttttcacacg gcttttgaat attgccgtta cccgaaggtc tggatt
//
LOCUS       DAWHSK010000390          643 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.390, whole genome shotgun sequence.
ACCESSION   DAWHSK010000390 DAWHSK010000000
VERSION     DAWHSK010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.390"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..518
                     /locus_tag="WIC03_002160"
     CDS             <1..518
                     /locus_tag="WIC03_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEZ3884603.1"
                     /translation="ENVRFAEKFGIPFIDKDDDYENDRKEWFAKAKGMEFEPERGIRC
                     TMCFDMRFEKAAQYAHEHGFPVFTSSLGISRWKNMAQINDCGHRAAAPYDDVVYWDFN
                     WRKGGGSARMIEISKRENFYQQEYCGCAYSLRDSNAHRKSQGRIPIKLGVLYYGDEST
                     QYEPAPIRVDK"
BASE COUNT          172 a          166 c          173 g          132 t
ORIGIN      
        1 aggaaaacgt gcgctttgcg gaaaagttcg gcattccttt catcgataaa gacgacgact
       61 acgaaaacga ccgcaaagaa tggtttgcca aagccaaagg catggagttt gagccggaac
      121 gcggcatccg ctgcaccatg tgtttcgata tgcgttttga aaaggcggcg caatacgcgc
      181 atgaacacgg gttccccgtc tttaccagtt cgctgggcat ttcacgctgg aaaaatatgg
      241 cgcaaatcaa cgactgcgga caccgcgccg ccgcgcctta cgatgatgtg gtgtattggg
      301 atttcaactg gcgcaaaggc ggcggcagcg cgcgcatgat tgaaatcagc aaacgtgaaa
      361 acttctacca gcaggaatat tgcggttgtg cctattccct gagggattcc aatgcccacc
      421 gcaaatcaca gggcagaatc cccatcaaac tcggcgtgct gtattacggc gacgaatcga
      481 cacaatacga acctgcaccc atccgggtgg acaaataaac atccgatgcc gtctgaaggt
      541 tcagacggca tcaggttcgg catcggcacg gggaaaggtt tgccggtttg gcaatctgca
      601 atcggaaacc gcattggcaa gtttgccgtt ttgataaaac acc
//
LOCUS       DAWHSK010000391          627 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.391, whole genome shotgun sequence.
ACCESSION   DAWHSK010000391 DAWHSK010000000
VERSION     DAWHSK010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..627
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.391"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(119..>627)
                     /locus_tag="WIC03_002161"
     CDS             complement(119..>627)
                     /locus_tag="WIC03_002161"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698318.1"
                     /note="AckA utilizes acetate and can acetylate CheY which
                     increases signal strength during flagellar rotation;
                     utilizes magnesium and ATP; also involved in conversion of
                     acetate to aceyl-CoA; also known to act on propionate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEZ3884604.1"
                     /translation="LEGLVMGTRSGDIDPSVFGFLAENANMTIAQITEMLNKKSGLLG
                     ISGLSNDCRTIEEEAAKGHKGAKLALDMFIYRLAKYIGSMAVAAGGLDALVFTGGIGE
                     NSDIIRERVIGYLGFLGLNIDQEANLKARFGNAGVITTAGSKAVAVVIPTNEELMIAH
                     DTARLSGL"
BASE COUNT          134 a          162 c          185 g          146 t
ORIGIN      
        1 gttcaggtaa gcggctgcaa ccgtcaacgt ctgaaaagag gcggtgctgt ttaagcatgg
       61 aaagccggat aaaaaaactg cctccgtttt cggaggcagt tcgcgtgggg attgaatctt
      121 acagaccgct caagcgggcg gtatcgtgtg caatcatcaa ttcctcgttg gtcggaatga
      181 ccacggcaac ggctttgctg ccggcagtgg taatcacgcc ggcgttgcca aagcgtgcct
      241 tcaggttggc ttcttggtcg atgttcagac cgaggaagcc caagtagccg atcacgcgtt
      301 cgcggatgat gtcggagttt tcgccgatgc cgccggtaaa gaccagtgcg tccaaaccgc
      361 ctgcggcaac cgccatactg ccgatgtatt tggcaaggcg gtagataaac atatccaagg
      421 ccaatttcgc gcctttatgc cccttggcgg cttcttcttc aatggtgcgg cagtcgttgg
      481 acaggccgga aatgccgagc agaccggatt ttttattcag catttcagtg atttgggcga
      541 tggtcatatt ggcgttttcg gcgaggaagc cgaatacgga aggatcgatg tcgccgctgc
      601 gcgtacccat taccagccct tccagcg
//
LOCUS       DAWHSK010000392          625 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.392, whole genome shotgun sequence.
ACCESSION   DAWHSK010000392 DAWHSK010000000
VERSION     DAWHSK010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.392"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..553
                     /locus_tag="WIC03_002162"
     CDS             <1..553
                     /locus_tag="WIC03_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HEZ3884605.1"
                     /translation="LTSRLQHLKVGDDVLISKKPTGTLVAGDLNPGKHLYLLSTGTGI
                     APFLSITKDPEIYEQFEKIILVHGVRYKKDLAYYDRFTKELPEHEYLGDLVKEKLIYY
                     PIVSREEFEHHGRLTDLMVSGKLFEDIGLPKINPQDDRAMLCGSPAMLKDTCKVLDDF
                     GLTVSPKTGVRGDYLIERAFVDQ"
BASE COUNT          175 a          178 c          145 g          127 t
ORIGIN      
        1 gctgaccagc cgcctgcaac acctcaaagt cggcgacgac gtgttaatca gcaaaaaacc
       61 gaccggaacc ctggttgccg gcgacctgaa tccgggcaaa cacctttact tgttgagcac
      121 cggtaccggc atcgctcctt tcttgagcat caccaaagac cccgaaattt acgagcaatt
      181 tgaaaaaatc atcctcgtac acggcgtgcg ctacaaaaaa gatttggcgt actacgaccg
      241 ctttaccaaa gaattgcccg aacacgaata cctcggtgac ttggttaaag aaaaactgat
      301 ttactacccg attgtttcgc gcgaagaatt cgaacaccac ggtcgcctaa ccgacctgat
      361 ggtgagcggc aaactgtttg aagacatcgg cctgcccaaa atcaacccgc aagacgaccg
      421 cgccatgctg tgcggcagcc ccgcgatgct gaaagatacc tgcaaagttt tggacgattt
      481 cggactgacc gtctctccga aaacaggcgt acgcggcgat tacctgattg agcgtgcgtt
      541 tgtagatcaa taagctgttt gaaaatcccg tctgaacggc accagcaatc aaaaaccccg
      601 aatcctgcca atcaggattc ggggt
//
LOCUS       DAWHSK010000393          624 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.393, whole genome shotgun sequence.
ACCESSION   DAWHSK010000393 DAWHSK010000000
VERSION     DAWHSK010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.393"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            23..>624
                     /locus_tag="WIC03_002163"
     CDS             23..>624
                     /locus_tag="WIC03_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ3884606.1"
                     /translation="MFNRPFEQREKNNKPHFVFCTVSHLRRLKGHDVLLTAFARALAQ
                     CPQLRLNIGGSGQEEQRLKQQAADLGITHAVTFLGALQPEAVLDLMKNSDAFVLASRT
                     ETFGVVYIEALSQGLPVIATRCGGAESIVSDGNGYLVPVDDDDALADALIKMYEHHSD
                     FEPARLRENCLNEFGENAVIGRLIGIFRQAIAEYGKKIPV"
BASE COUNT          164 a          172 c          154 g          134 t
ORIGIN      
        1 cctccccaac atactgggcg gaatattcaa ccgaccgttt gaacaaagag aaaaaaacaa
       61 caaaccgcat ttcgtgttct gcaccgtctc gcaccttcgc cgtctcaaag gacacgatgt
      121 cctgctcact gcctttgccc gggcgttggc acaatgcccg caactgcgtc taaacatcgg
      181 cggcagcgga caggaagaac agcggctgaa acagcaggcg gcagacttgg gcattaccca
      241 tgccgttaca tttttgggcg cactgcaacc cgaagcagtc ttggatttga tgaagaacag
      301 cgacgcattt gtccttgcca gccgcaccga aaccttcggc gtggtctata tcgaagcact
      361 gtcccaagga ttgcccgtca ttgcaacacg ctgcggcggt gcggaatcta ttgtttcaga
      421 cggcaacgga tatttggttc ctgttgacga cgacgatgcc cttgccgacg cactcatcaa
      481 aatgtatgaa caccactctg atttcgaacc tgcccgactt agagaaaact gtctgaacga
      541 atttggcgaa aatgccgtta taggcaggtt gatcggcatt ttccgacagg caatcgcaga
      601 atacggtaag aaaataccgg tgaa
//
LOCUS       DAWHSK010000394          615 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.394, whole genome shotgun sequence.
ACCESSION   DAWHSK010000394 DAWHSK010000000
VERSION     DAWHSK010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..615
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.394"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..>615
                     /locus_tag="WIC03_002164"
     CDS             <1..>615
                     /locus_tag="WIC03_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HEZ3884607.1"
                     /translation="SSHVACPRCAGTGVIRGIESTALHVLRIIQEEAMKDNTGEVRAQ
                     VPVDVATFLLNEKRAELFAMEERLDVNVVLIPNIHLENPHYEINRIRTDDVEEDGEPS
                     YKRVAEPEEDESAKPFGGEKAKAARPEPAVKGVRHTSPAPTAAPEKKTSWWDSFKAWL
                     KRIFGGSETQAAPAAETSEKRSTANRSGSRANNRRQNPRRSKREG"
BASE COUNT          164 a          217 c          162 g           72 t
ORIGIN      
        1 aagcagccac gtcgcctgtc cgcgctgcgc cggcaccggc gtgattcggg gcatcgaatc
       61 caccgccctg cacgttttac gcatcattca agaagaagcg atgaaggaca acaccggaga
      121 agtgcgcgca caagtgcccg tcgatgtcgc caccttcctg ctgaacgaaa aacgcgccga
      181 gctgtttgcg atggaagagc gtttggatgt caacgtcgtc ctgattccga acatccacct
      241 cgaaaatccg cactacgaaa tcaaccgcat ccgcaccgac gacgtagaag aagacggcga
      301 accgagctac aaacgcgtcg ccgagccgga agaagacgaa tccgccaaac cgttcggcgg
      361 cgaaaaagcc aaagccgccc gtcccgaacc cgccgtcaaa ggcgtgcgcc acaccagccc
      421 cgccccgact gccgcccccg agaaaaaaac ctcttggtgg gacagcttca aagcatggct
      481 caaacgcatt ttcggcggca gcgaaaccca agccgcgccc gctgccgaaa cctccgaaaa
      541 acgcagcacg gcaaaccgca gcggcagccg cgccaacaac cgccgccaaa acccgcgccg
      601 cagcaaacgc gaagg
//
LOCUS       DAWHSK010000395          609 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.395, whole genome shotgun sequence.
ACCESSION   DAWHSK010000395 DAWHSK010000000
VERSION     DAWHSK010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..609
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.395"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..452
                     /locus_tag="WIC03_002165"
     CDS             <1..452
                     /locus_tag="WIC03_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEZ3884608.1"
                     /translation="LKETQDNWQELKTENQENGSFHAASSLGLSAIYDFKLNDKFKPY
                     IGARVAYGHVKHQVHSVETKTTTVTSKPNGKNLKGGPVSSREPSKPPYHESHSTSSLG
                     LGVIAGVGFDITPKLTLDTGYRYHNWGRLENTRFKTHEVSLGVRYRF"
BASE COUNT          171 a          185 c          133 g          120 t
ORIGIN      
        1 tgttgaaaga aacccaggac aactggcaag aactgaagac ggaaaatcag gaaaacggca
       61 gcttccacgc cgcttcttct ctcggcttat ccgccattta cgatttcaaa ctcaacgata
      121 aattcaaacc ctatatcggt gcgcgcgtcg cctacggaca cgttaaacat caggttcatt
      181 cggtggaaac aaaaaccacg actgttacct ctaaaccaaa cggaaaaaat ctaaagggag
      241 gacctgtctc atcaagagag cccagcaaac ctccctatca cgaaagccac agcaccagca
      301 gcttgggtct tggtgtcatc gccggtgtcg gtttcgacat cacgcccaag ctgaccttgg
      361 acaccggata ccgctaccac aactggggac gcttggaaaa cacccgcttc aaaacccacg
      421 aagtctcatt gggcgtgcgc taccgcttct gattccccga caccgatgcc gtctgaacct
      481 tcagacggca tttttgatgc actcgccgtt tacaggcgcg gggcgggcgt ggaaataccc
      541 gaaccgtcat tcccgacaac accgtaatct tgaaacccgc cattcccgat aacagcgcaa
      601 tcttgaaac
//
LOCUS       DAWHSK010000396          585 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.396, whole genome shotgun sequence.
ACCESSION   DAWHSK010000396 DAWHSK010000000
VERSION     DAWHSK010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.396"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(225..410)
                     /locus_tag="WIC03_002166"
     CDS             complement(225..410)
                     /locus_tag="WIC03_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884609.1"
                     /translation="MPEQARARPIQTAMTLCRRFGAGWRHTRRLPRFISHRSSETRNP
                     PGPTCGMKRLSWREVFY"
     gene            complement(522..>585)
                     /locus_tag="WIC03_002167"
     CDS             complement(522..>585)
                     /locus_tag="WIC03_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M48 family peptidase"
                     /protein_id="HEZ3884610.1"
                     /translation="YTPEAKQWLKIHGRELFALG"
BASE COUNT          112 a          163 c          183 g          127 t
ORIGIN      
        1 cccactttcg tgggaatgac gggatgcagg ttttcttaac cccgcgttct agattcccac
       61 tttcgtagga atgacggcgg tagatttggc agatgcggcg gattcggcag gtctcaaccc
      121 atcctacaat ccaccctgac cgccttttac gaatccgccg ccatcctggg aaacgcaaaa
      181 aaatgccgtc cgaaaacctt tcagacggca ttttcgcggg caaatcagta aaagacttcg
      241 cgccagctta agcgtttcat accgcacgtc ggaccgggcg ggtttcgggt ttcggaagag
      301 cggtgtgaaa tgaaacgcgg cagacggcgc gtgtgccgcc atcctgcccc gaaccggcgg
      361 caaagcgtca tcgcggtttg aatcgggcgg gcgcgtgcct gttccggcac ggatgccgtc
      421 tgaaagcggc ggcagcggta tgtgtcgccg gctgtgatgc tgctttccgg atgtccgttt
      481 gggaggaggt ctgaattggc ggatgccggc ccgggaccgc gtcagcctaa ggcgaaaagt
      541 tccctgccgt ggattttgag ccactgtttc gcttcgggcg tgtag
//
LOCUS       DAWHSK010000397          585 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.397, whole genome shotgun sequence.
ACCESSION   DAWHSK010000397 DAWHSK010000000
VERSION     DAWHSK010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.397"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            211..>585
                     /locus_tag="WIC03_002168"
     CDS             211..>585
                     /locus_tag="WIC03_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine synthase A"
                     /protein_id="HEZ3884611.1"
                     /translation="MDTAQKQRWAITLSYDGSRFYGWQKQADGVPTVQAALETALAQI
                     AGESVATTVAGRTDTGVHATAQVVHFDTTAARPQQAWVRGVNAHLPEGIAVLHARQVA
                     PEFHARFDAYGRHYRYLLESAPV"
BASE COUNT          130 a          203 c          141 g          111 t
ORIGIN      
        1 ggtttcttta gattttacgt cctagattcc cgactgcgcg ggaatgacga ttcgggcact
       61 cctgacaggg tgaattcaca ggatagcgat tcgtagcaac tgcatccccc ccaactcccc
      121 aaacaacgcc gctcgccccg ggcgtttgcc gtttccctgc aaaatctgcg atacaatgca
      181 gtctgaacat ttatccgaat cccaaatccg atggataccg cacaaaaaca acgttgggca
      241 ataaccctat cctatgacgg cagccgcttt tacggctggc agaaacaggc tgacggcgta
      301 ccgaccgttc aggcggcatt ggaaaccgcg ctcgcccaaa tcgcaggaga atctgtcgcc
      361 accaccgttg ctggcaggac cgacaccggc gtgcatgcca ccgcccaagt cgtccacttc
      421 gacacaactg ccgcccgtcc ccaacaggca tgggtgcgcg gcgtaaatgc ccacctgccc
      481 gaaggcattg ccgttttgca cgcccgacag gtcgcccccg aatttcacgc ccgcttcgac
      541 gcatacggac ggcattaccg ctacctgctc gaatccgccc ccgtc
//
LOCUS       DAWHSK010000398          584 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.398, whole genome shotgun sequence.
ACCESSION   DAWHSK010000398 DAWHSK010000000
VERSION     DAWHSK010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.398"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..561
                     /locus_tag="WIC03_002169"
     CDS             <1..561
                     /locus_tag="WIC03_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 49 family protein"
                     /protein_id="HEZ3884612.1"
                     /translation="RNIDNIANDTEAFVNRYDLNRHMIYNTLLMVEGKQLDRLKQRSE
                     KVLAHPTPSKWLQKRLYDYRFFLAFAEQDAEAMKAALEPLFDKKTARMAAKETLSYFD
                     FYLQPQIVTYAKIASMHGFDLGIDHEIAPRDLTVYDPLPADEYQDIFDFMKQYDLSYP
                     YEYLQDWIDYYTFKTDKLVFGNAKRE"
BASE COUNT          163 a          145 c          134 g          142 t
ORIGIN      
        1 cgcaatatcg acaacatcgc caacgataca gaagccttcg taaaccgata cgacctcaac
       61 cgtcatatga tttacaatac tctgctgatg gtggagggta agcagcttga tcggttgaaa
      121 caacgtagcg agaaagtctt ggcgcatccc acccctagca aatggctgca aaagcggttg
      181 tacgattacc gcttcttcct cgctttcgcc gaacaggatg ccgaggcgat gaaggccgcc
      241 ttagagccgc tttttgataa aaaaaccgcg cgtatggctg ccaaagaaac attgtcctat
      301 ttcgatttct acctgcagcc gcaaatcgtt acctacgcca aaatcgcatc catgcacggt
      361 ttcgatttgg gcatagacca cgaaatcgcg ccgagggatt tgactgttta cgatccgctg
      421 ccggcagacg aatatcaaga catcttcgat tttatgaaac agtatgactt gtcttacccg
      481 tatgaatatc tgcaggattg gatagattac tatacgttca aaaccgataa gctggtattt
      541 ggtaacgcga agcgagagtg agccgtaaaa ctctgagctc ctgt
//
LOCUS       DAWHSK010000399          571 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.399, whole genome shotgun sequence.
ACCESSION   DAWHSK010000399 DAWHSK010000000
VERSION     DAWHSK010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.399"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..86
                     /locus_tag="WIC03_002170"
     CDS             <1..86
                     /locus_tag="WIC03_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HEZ3884613.1"
                     /translation="QNRPDFKQNNGKNPDVGNEVIRRRKGG"
     gene            90..>571
                     /gene="mafB"
                     /locus_tag="WIC03_002171"
     CDS             90..>571
                     /gene="mafB"
                     /locus_tag="WIC03_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HEZ3884614.1"
                     /translation="MKPLRRLTNLLAACAVAAAALIQPALAADLAQDPFITDNAQRQH
                     YEPGGKYHLFGDPRGSVSDRTGQINVIQDYTHQMGNLLIQQANINGTIGYHTRFSGHG
                     HEEHAPFDNHAADSASKEQGNVDEGFTVYRLNWEGHEHHPADAYDGPKGGNYPKPTGA
                     R"
BASE COUNT          157 a          210 c          134 g           70 t
ORIGIN      
        1 cccaaaaccg tcccgacttc aaacaaaaca acggtaaaaa ccccgatgtc ggcaacgaag
       61 tcatccgccg ccgcaaagga ggataaaccg tgaaaccgct gcgcagactg acaaacctcc
      121 ttgccgcctg cgccgtagcg gcggccgcac tcatacagcc cgccctcgcg gcggacttgg
      181 cgcaagaccc gttcattacc gataacgccc aacggcagca ctacgaaccc ggcggcaaat
      241 accacctctt cggcgacccg cgcggcagcg tttccgaccg caccggtcaa atcaacgtca
      301 tccaagacta tacccaccag atgggcaacc tgctcatcca acaggcaaac atcaacggca
      361 caatcggcta ccacacccgc ttttccggac acggacacga agaacacgcc cccttcgaca
      421 accacgccgc cgacagcgca agcaaagaac aaggcaacgt tgacgaaggc tttaccgtgt
      481 accggctcaa ctgggaagga cacgagcacc atcccgccga tgcctacgac ggcccgaagg
      541 gcggcaatta ccccaaacct acgggtgcac g
//
LOCUS       DAWHSK010000400          557 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.400, whole genome shotgun sequence.
ACCESSION   DAWHSK010000400 DAWHSK010000000
VERSION     DAWHSK010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.400"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            157..>557
                     /locus_tag="WIC03_002172"
     CDS             157..>557
                     /locus_tag="WIC03_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884615.1"
                     /translation="MKTDIQTELTQALLPHEKVWANEEKTILAKNILLDLVEKTDPTI
                     IGLLLNNDELKRHFFVEVNGVLVFKLQDLRFFLDKHSVNNSYTKYANRIGLTDGNRFL
                     KDNSDIVLDFPFKDCVLNGGQSTEEGEEIYF"
BASE COUNT          186 a          108 c          113 g          150 t
ORIGIN      
        1 gacacactcg gacacccgcc ccgcttatcg caactttgcg aacgccccgg aaacagcaaa
       61 tacagcaaat aattgatttt attagagttt atttgcaaaa ccatttgccg ttacacaaga
      121 atggcacatt aaaatgactg atgaggattt ataacgatga agacagacat tcaaaccgaa
      181 ttaacccaag ccctactacc acacgaaaaa gtatgggcga acgaagaaaa aaccatttta
      241 gccaaaaaca ttctgttgga tttggtggaa aaaaccgacc cgaccattat cggtttgtta
      301 ttgaataatg atgagttaaa acgccatttc tttgtggaag tgaatggtgt tttggtgttt
      361 aaattgcagg atttacgttt tttcttggac aaacacagcg tcaataattc ctacacaaaa
      421 tacgccaacc gcattggttt gacggacggc aaccgctttt tgaaagacaa ttcggatatt
      481 gtgttggatt ttccgtttaa agattgcgta ttgaatggcg gacaaagcac cgaagaaggc
      541 gaagagattt attttaa
//
LOCUS       DAWHSK010000401          549 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.401, whole genome shotgun sequence.
ACCESSION   DAWHSK010000401 DAWHSK010000000
VERSION     DAWHSK010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.401"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            158..487
                     /locus_tag="WIC03_002173"
     CDS             158..487
                     /locus_tag="WIC03_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ3884616.1"
                     /translation="MGSLIIEDLQEGFGKEAVKGKEITVHYTGWLEDGTKFDSSLDRR
                     QPLTITLGVGQVIKGWDEGFGGMKEGGKRKLTIPSEMGYGARGAGGVIPPHATLIFEV
                     ELLKVYE"
BASE COUNT          163 a          139 c          147 g          100 t
ORIGIN      
        1 atgaaaaagc atggaacctt tacgaaaaaa ataaaacagt ccaacaaaca cagccgtttc
       61 aactgcaccg gtgcggctga cgtttgagcg gcatttgact tcgccggacg gtatgttacc
      121 cttgccgttt tgaccaacaa gcaacgaaag gacaaatatg ggcagcctga ttattgaaga
      181 tttgcaggaa ggcttcggaa aagaagcagt taaaggcaaa gagattaccg tgcattacac
      241 aggttggctg gaagacggca ccaaattcga ctccagcctc gaccgccgcc agccgctgac
      301 catcacgctc ggcgtcggac aagtcatcaa aggctgggac gaaggcttcg gcggcatgaa
      361 ggaaggcggc aaacgcaagc tgaccatccc ttcggaaatg ggctacggcg cacgcggcgc
      421 gggcggcgtg attcctccgc acgccacttt gatatttgaa gtcgagctgc tgaaagtgta
      481 cgaataaagc tgcccgggca ataccgtctg acataatggc ttcaagcaac gtaaacaacg
      541 aaatcaaag
//
LOCUS       DAWHSK010000402          537 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.402, whole genome shotgun sequence.
ACCESSION   DAWHSK010000402 DAWHSK010000000
VERSION     DAWHSK010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.402"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
BASE COUNT          175 a          106 c          125 g          131 t
ORIGIN      
        1 cagtgtcatg agaaattttc ctttgtcaag tgttaaaatt ataatgatta tatactacat
       61 cacactacat cgcaatgaaa aatcaggaaa aacaaaaaac ctctccgccg tcattcccgc
      121 gcaggcggaa atctagaatt ttaatgcggc aagaatttat cggaaaaaac tgaagtttaa
      181 agacctagat tcccgcctgc gcgggaatga cggtttagaa atcacctgaa acgagaaaaa
      241 gaaccgaaac cggacaagtc ggattcccgc acgggcggga aggacggaat ttcagattgt
      301 tgtttttgat tttctgtttt gtgggaataa cgggattttg gatgatgtgc atttatcggg
      361 aaaactaaaa accctccgcc gtcattcccg tgaaagcggg aatctagaat ttcaatgccg
      421 caagaattta tcggggatgt ccggggttta aaggtctgga ttcccgcctg cacgggaatg
      481 acggtttaga aatcacctga aacgagaaaa agaaccgaaa ccagataagt cggattc
//
LOCUS       DAWHSK010000403          525 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.403, whole genome shotgun sequence.
ACCESSION   DAWHSK010000403 DAWHSK010000000
VERSION     DAWHSK010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.403"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            368..>525
                     /locus_tag="WIC03_002174"
     CDS             368..>525
                     /locus_tag="WIC03_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEZ3884617.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNRQKTR
                     YLRDHRGRP"
BASE COUNT          160 a          134 c          102 g          129 t
ORIGIN      
        1 gcatccgccc atacccgtat caatatcgtt gaacttccat tttttaccct gcagcggatg
       61 tgcggttaaa tattttggcg gaaaagcaaa gaagtatact tctttttagt tatggttgat
      121 tccataacta aaaaagtatc gataaaccca ttttgcgagg tacggataat gtctgacagc
      181 tttgaaaaca gccccgaata cgataattgg atcgaatcgg gcggtcgtga tgaagattac
      241 gaatactact acaacaaatg gcaacgcagg acgcgctgac cattaaaccc tgacaaaccg
      301 gtttgagacc tttgcaaaat tccccaaaat cccctaaatt cccaccaaga catttaggag
      361 atttctcatg agcaccttct tccagcaaac cgcccaagcc atgattgcca aacacatcga
      421 ccgtttcccg ctattgaagt tggatcaggt aattgattgg cagccgatcg aacagtacct
      481 gaatcgtcaa aaaacccgtt acctcagaga ccaccgcggc cgtcc
//
LOCUS       DAWHSK010000404          519 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.404, whole genome shotgun sequence.
ACCESSION   DAWHSK010000404 DAWHSK010000000
VERSION     DAWHSK010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.404"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            58..357
                     /locus_tag="WIC03_002175"
     CDS             58..357
                     /locus_tag="WIC03_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEZ3884618.1"
                     /translation="MQRCFNWFDHYCRRLEKYFRRCSIMETWVQNYTAIGGSLYLTAA
                     AALLPIVFFFAALTVLKLKGYQAGLYTLLIALAVAVFGFGMPAGMAVSSLPPQPH"
     gene            351..519
                     /locus_tag="WIC03_002176"
                     /pseudo
     CDS             351..519
                     /locus_tag="WIC03_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777280.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
BASE COUNT          116 a          148 c          119 g          136 t
ORIGIN      
        1 gtactggttt aaatttaatc cattctattg acctgaatat taaagtttcg caaagtaatg
       61 caacgttgct ttaattggtt tgaccactat tgccgacgat tagaaaaata ttttcggaga
      121 tgttcaatta tggaaacttg ggttcaaaac tacacggcaa tcggcggcag cctgtatctg
      181 actgccgccg ccgcactctt acccatcgtc tttttctttg ccgcgctgac cgtcctgaag
      241 ctgaaaggct atcaggcggg gctttatacg ctgctgattg cgcttgccgt tgccgtattc
      301 ggcttcggga tgccggcggg tatggcggtt tcttccctgc cgccgcagcc gcactgaccc
      361 aacgccccta cgccacgctg gatttgaccg cgcattacaa aatcggcaaa tccacccgca
      421 ttggtttgga ctttgaaaac gtgttcaaca aacgctaccg ccctatgccc gacattcacg
      481 tttacggcac gccgcgcagc ctgaccgcaa ccgtcaaac
//
LOCUS       DAWHSK010000405          517 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.405, whole genome shotgun sequence.
ACCESSION   DAWHSK010000405 DAWHSK010000000
VERSION     DAWHSK010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.405"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..>517
                     /locus_tag="WIC03_002177"
     CDS             <1..>517
                     /locus_tag="WIC03_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ3884619.1"
                     /translation="YRNWLAQDDTLSELLELINCQLTEKGLKVEKASAAVIDATIIQT
                     AGSEQRQAIEVDEEGQVSGQTTLSKDKNARWTKKNSFYKLGYKQHTRTDAEGYIEKLY
                     ITPTNAHECTHLSPLLEGLPKGTTVYADKGYDSKENRQHLKEHRLQNGIMRKACRNRP
                     LTETQTKRNRY"
BASE COUNT          169 a          145 c          121 g           82 t
ORIGIN      
        1 gctaccgcaa ctggctggcg caagacgaca ccctgtccga actgttggaa ctgattaact
       61 gccaactgac cgaaaaaggc ttaaaagtag agaaagcatc cgccgccgtc attgacgcca
      121 ccattattca gaccgccggc agcgaacagc gtcaggccat agaagtcgat gaagagggac
      181 aagtcagcgg tcaaaccaca ctgagtaagg acaaaaatgc ccgttggaca aagaaaaaca
      241 gcttctacaa actcggttac aaacaacata cccgtaccga tgcggaaggc tatatcgaga
      301 aactgtacat cactcccacc aatgcccatg agtgcacaca cctgtcgccg ttgttggaag
      361 gactgcccaa aggtacgacc gtctatgccg acaaaggcta cgacagcaag gaaaaccggc
      421 aacatctgaa agaacatcgg ttgcagaacg gcattatgcg caaagcctgc cgcaaccgtc
      481 cgctgacgga aacgcaaacc aaacgtaacc gatattt
//
LOCUS       DAWHSK010000406          510 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.406, whole genome shotgun sequence.
ACCESSION   DAWHSK010000406 DAWHSK010000000
VERSION     DAWHSK010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.406"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..>510
                     /locus_tag="WIC03_002178"
     CDS             <1..>510
                     /locus_tag="WIC03_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Opacity protein opA54"
                     /protein_id="HEZ3884620.1"
                     /translation="THDYPKATGANNTSTVSDYFRNIRTHSIHPRVSVGYDFGDWRIA
                     ADYASYRKWNNNKYSVNTKNVQKNDNGNRQDLKTENQENGTFHAVSSLGLSAIYDFKL
                     NDKFDKFKPYIGARVAYGHVKHQVHSVKKETTTVFTAPTGGAKVPGKVVKGPTSKPPY
                     HESHSTSSLG"
BASE COUNT          171 a          138 c          105 g           96 t
ORIGIN      
        1 tacccacgat tatccgaaag caaccggtgc aaacaacaca agcacagtaa gcgattattt
       61 cagaaacatc cgtacgcatt ccatccatcc ccgtgtgtcg gtcggctacg atttcggcga
      121 ctggaggata gcggcagatt atgccagtta cagaaaatgg aacaacaata aatattccgt
      181 caacacaaaa aatgtgcaaa aaaacgacaa tggcaacagg caagacctga agacggaaaa
      241 tcaggaaaac ggtacattcc acgccgtctc atcgctcggc ttatccgcca tttacgattt
      301 caaactcaac gataaattcg ataaattcaa accctatatc ggcgcgcgcg tcgcctacgg
      361 acacgttaaa catcaggttc attcagtgaa aaaagaaacc acgactgttt tcactgcacc
      421 aacgggaggc gctaaagtgc caggcaaggt cgtaaaaggt ccgacctcca aacctcccta
      481 tcacgaaagc cacagcacca gcagcttggg
//
LOCUS       DAWHSK010000407          503 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.407, whole genome shotgun sequence.
ACCESSION   DAWHSK010000407 DAWHSK010000000
VERSION     DAWHSK010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.407"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..>503)
                     /locus_tag="WIC03_002179"
     CDS             complement(<1..>503)
                     /locus_tag="WIC03_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714702.1"
                     /note="FabB; beta-ketoacyl-ACP synthase I, KASI; catalyzes
                     a condensation reaction in fatty acid biosynthesis:
                     addition of an acyl acceptor of two carbons from
                     malonyl-ACP; required for the elongation of short-chain
                     unsaturated acyl-ACP; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HEZ3884621.1"
                     /translation="FGIRQNASCENIVDWHERFPELEAQLGAPIEDYAPPKHWTRKQL
                     RSMGRVSYLCVDAAEQALTDAGLLGDESITDGRMGVACGSSSGSTKDIGDVGELLLTG
                     TSRNFSANTYVRMMPHTAAANIGIFFGLKGRIIPTSSACSSGSQGIGYAYEAIKYGLT
                     DMMLAGG"
BASE COUNT          103 a          157 c          131 g          112 t
ORIGIN      
        1 ctccgcccgc cagcatcata tcggtcagac cgtatttgat ggcttcgtag gcataaccta
       61 tgccttggct gccggacgaa cacgcgctcg atgtcgggat gatgcgtcct ttcagtccga
      121 aaaagatgcc gatattggcg gcggcggtgt gcggcatcat acgcacatag gtgttggcac
      181 tgaagttgcg cgacgtgccg gtcagcaaca attcgcccac atcgccgatg tctttggtgc
      241 tgccgctgga agagccgcag gcaacgccca tccgtccgtc ggtaatgctt tcgtctccga
      301 gcaaaccggc atccgtcaaa gcctgctccg ccgcatcgac gcacaaatac gacacacgcc
      361 ccatacttct gagctgcttg cgcgtccaat gtttcggcgg cgcgtaatcc tcaatcggcg
      421 cacccagttg cgcttccaat tcggggaaac gttcgtgcca atccactata ttttcgcaag
      481 acgcgttttg cctgattcca aaa
//
LOCUS       DAWHSK010000408          475 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.408, whole genome shotgun sequence.
ACCESSION   DAWHSK010000408 DAWHSK010000000
VERSION     DAWHSK010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.408"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..>475
                     /locus_tag="WIC03_002180"
     CDS             <1..>475
                     /locus_tag="WIC03_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEZ3884622.1"
                     /translation="NTKEVGRNNNNNIDIWKELKTENQENGSFHAASSLGLSAIYDFK
                     LNDKFDKFKPYIGARVAYGHVKHQVHSVRKETTTVFSKPSGSVTKPGEIVETPTSKPA
                     YHESHSISSLGLGVIAGVGFDITPKLTLDTGYRYHNWGRLENTRFKTHEVSLGMRY"
BASE COUNT          147 a          127 c          105 g           96 t
ORIGIN      
        1 aacacaaaag aggtgggaag aaacaataac aataacattg acatttggaa agaactgaag
       61 acggaaaatc aggaaaacgg cagcttccac gccgcttctt ctctcggctt atccgccatt
      121 tacgatttca aactcaacga taaattcgat aaattcaaac cctatatcgg tgcgcgcgtc
      181 gcctacggac acgttaagca tcaggttcat tcggtgagaa aagaaaccac gactgttttc
      241 agtaaaccaa gtggtagcgt tacaaagcca ggcgagatcg tagaaactcc gaccagcaaa
      301 cctgcctatc acgaaagcca cagtatcagc agcttgggtc ttggtgtcat cgccggtgtc
      361 ggtttcgaca tcacgcccaa gctgaccttg gacaccggat accgctacca caactgggga
      421 cgcttggaaa acacccgctt caaaacccac gaagtctcat tgggcatgcg ctacc
//
LOCUS       DAWHSK010000409          475 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.409, whole genome shotgun sequence.
ACCESSION   DAWHSK010000409 DAWHSK010000000
VERSION     DAWHSK010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.409"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..372
                     /locus_tag="WIC03_002181"
     CDS             <1..372
                     /locus_tag="WIC03_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEZ3884623.1"
                     /translation="VAYTSPEVAWVGETELSAKASGRKITKANFPWAASGRAIANGCD
                     NGFTKLIFDAETGRIIGGGIVGPNGGDMIGEVCLAIEMGCDAADIGKTIHPHPTLGES
                     IGMAAEVALGTCTDLPPQKKK"
BASE COUNT          114 a          132 c          128 g          101 t
ORIGIN      
        1 gttgcctaca cttcccccga agtggcgtgg gtgggcgaaa ccgagttgtc cgccaaagcc
       61 tccggccgca aaatcaccaa agccaacttc ccgtgggcgg cttccggtcg tgcgattgcc
      121 aacggttgcg acaacggctt taccaagctg attttcgatg ccgaaaccgg ccgcatcatc
      181 ggcggcggca ttgtcggtcc gaacggtggc gacatgatcg gcgaagtctg ccttgccatc
      241 gaaatgggct gcgacgcggc agacatcggc aaaaccatcc acccgcaccc gaccttgggc
      301 gaatccatcg gtatggcggc ggaagtggca ttgggtactt gtaccgacct gcctccgcaa
      361 aagaaaaaat aaatccgact gaataaacag ccgataaggt ttatttgagc aaatgccgtc
      421 tgaaatgttc agacggcatt ttctatttta cagcggatta aaatattttt tccgg
//
LOCUS       DAWHSK010000410          474 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.410, whole genome shotgun sequence.
ACCESSION   DAWHSK010000410 DAWHSK010000000
VERSION     DAWHSK010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.410"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..185
                     /locus_tag="WIC03_002182"
     CDS             <1..185
                     /locus_tag="WIC03_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884624.1"
                     /translation="SEDWIRQVITNLPDNHPTKNILREAVRSGKIKTAVTGVDRQTGK
                     AVIIPVKVPSKANIRR"
     gene            188..>474
                     /locus_tag="WIC03_002183"
     CDS             188..>474
                     /locus_tag="WIC03_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma"
                     /protein_id="HEZ3884625.1"
                     /translation="MGYNMMTTQKCHEHITDVIIGTTQRFNTEKSTDSIEYINERKGS
                     PIGAICIVSIDVYAACSHAYLFEHNLKKFKQYAYAYSKLEILASMGWSDPTP"
BASE COUNT          170 a           83 c           91 g          130 t
ORIGIN      
        1 tgagtgagga ttggattaga caagttatta ctaacttacc agataatcat cccactaaaa
       61 acatccttcg agaagcagta cgttcgggta aaatcaaaac tgcggttact ggagtagatc
      121 gtcaaacagg taaagcagtt attattcctg ttaaagttcc ttctaaagcc aatataagga
      181 gataacaatg ggatacaata tgatgaccac ccaaaaatgt catgagcata ttacagatgt
      241 aattataggc accactcaaa gatttaatac agagaaaagt actgatagta tagagtatat
      301 taatgaaaga aaaggatctc caattggagc aatctgtatc gtttctatag atgtttatgc
      361 ggcatgctca catgcttatc tgtttgaaca caaccttaaa aagttcaaac agtatgctta
      421 tgcttacagt aaacttgaga tactggcaag tatgggatgg tctgacccca cgcc
//
LOCUS       DAWHSK010000411          454 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.411, whole genome shotgun sequence.
ACCESSION   DAWHSK010000411 DAWHSK010000000
VERSION     DAWHSK010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.411"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            1..454
                     /locus_tag="WIC03_002184"
                     /pseudo
     CDS             1..454
                     /locus_tag="WIC03_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          144 a          127 c          108 g           75 t
ORIGIN      
        1 tggcgcaaga cgacaccctg tccgaattgc tcaaactgat taaccgacaa ctgaccgaaa
       61 aaggtttaaa aatagagaaa gcatccgccg ccgtcattga cgccaccatt attcagaccg
      121 ctggcagcaa acagcgccag gccatagaag ttgacgaaga aggacaaatc agcggccaaa
      181 ccacaccgag taaagacaaa gatgcccgtt ggatctctac aaactcggtt acaaacaaca
      241 tacccgtacc gatgcggaag gctatatcga gaaactgcac attacccccg ccaatgccaa
      301 tgagtgcaaa cacctgtcgc cgttgttgga aggactgccc gaaggtacga ccgtctatgc
      361 cgacaaaggc tatgacagtg cggaaaaccg gcaacatctg gaagagcgtc agttgttaga
      421 cggcattatg cgcaaagcct gccgcaaccg tccg
//
LOCUS       DAWHSK010000412          453 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.412, whole genome shotgun sequence.
ACCESSION   DAWHSK010000412 DAWHSK010000000
VERSION     DAWHSK010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.412"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..293
                     /locus_tag="WIC03_002185"
     CDS             <1..293
                     /locus_tag="WIC03_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581389.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEZ3884626.1"
                     /translation="LKAGSLAAKIYGSEHIRERHRHRYEVNNNYVPTLEQAGLVVGGV
                     SAGRERLVETIELPNHPWFFACQFHPEFTSNPRKGHPLFTAFVKAALNNKKA"
BASE COUNT          128 a          122 c           98 g          105 t
ORIGIN      
        1 agttgaaagc aggcagcctc gccgccaaaa tctacggcag cgaacacatc cgcgaacgcc
       61 accgccaccg ctacgaagtc aacaacaact acgttcctac gctggaacaa gcaggtttgg
      121 tcgtcggcgg cgtatccgca ggacgcgaac gcttggtcga aaccatcgaa ctgccgaacc
      181 atccttggtt cttcgcctgc cagtttcatc ccgaattcac ttccaacccg cgcaaagggc
      241 atcccttgtt caccgcgttt gtcaaagctg cgttgaacaa taaaaaagcc tgataaagcg
      301 ttacttgatg atcaaaatgc cgtctgaaag cctgacaaag actttcagac ggcattttcg
      361 caagtcgggg attataaaag ccatcaattt aatttttatg gttttatcta atatttacca
      421 actgaatatt gttaagcggg gcgtgaaact caa
//
LOCUS       DAWHSK010000413          451 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.413, whole genome shotgun sequence.
ACCESSION   DAWHSK010000413 DAWHSK010000000
VERSION     DAWHSK010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.413"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..>451
                     /locus_tag="WIC03_002186"
     CDS             <1..>451
                     /locus_tag="WIC03_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30-like element IS1655 family transposase"
                     /protein_id="HEZ3884627.1"
                     /translation="VDQKSRIGDWEADTIVGKGQKSALLTLVERVTRYTIICKLDSLK
                     AEDTARAAVRALKAHKDRVHTITMDNGKEFYQHTKITKALKAETYFCRPYHSWEKGLN
                     ENTNGLIRQYFPKQTDFRNISDREIRRVQDELNHRPRKTLGYETPSVL"
BASE COUNT          147 a          116 c          102 g           86 t
ORIGIN      
        1 cgtcgaccag aaatcccgta tcggcgattg ggaagccgac accattgtcg gcaaaggaca
       61 gaaaagcgca ttattgacct tggtcgaacg cgttacccgc tacaccatca tctgcaaatt
      121 ggatagcctc aaagccgaag acactgcccg ggcagctgtt agggcattaa aggcacataa
      181 agacagggtg cacaccatta ccatggataa cggcaaagag ttctaccaac acaccaaaat
      241 aaccaaagca ttgaaagcgg agacttattt ttgtcgccct taccattctt gggagaaagg
      301 gctgaatgag aacaccaacg gactcatccg gcaatacttc cccaaacaaa ccgatttccg
      361 taacatcagt gatcgggaga tacgcagggt tcaagatgag ttgaaccacc gaccaagaaa
      421 aacacttggc tacgaaacgc caagtgtttt a
//
LOCUS       DAWHSK010000414          445 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.414, whole genome shotgun sequence.
ACCESSION   DAWHSK010000414 DAWHSK010000000
VERSION     DAWHSK010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.414"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
BASE COUNT          158 a           64 c           65 g          158 t
ORIGIN      
        1 gctgtactgg tttaaattta atccactata ttttgtaata gctgccatta tgcctactag
       61 gatatatacc ttctatataa tccttattta aataaaaacc cttataaaaa tctgtgttat
      121 ctgtcgggat tgaaaaaaag attttatctt tctcatcagg tttatatttg ctatcaatat
      181 caaaaagaga ataaggatta caatgagttc gacatactag gtaaaaattt atatcttgga
      241 tcatgaaata ttcgatattt atcaggatcg aaagatgcta aaaattcatc aatatcggta
      301 ctggatattt tgtcatcact aataaacatc gctagagatg ggcgactttg attttccatt
      361 tgttgattag taaaggatat aaatactaac ttgccattgt caaaccacat agatttatat
      421 agtggattaa atttaaacca gtacg
//
LOCUS       DAWHSK010000415          440 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.415, whole genome shotgun sequence.
ACCESSION   DAWHSK010000415 DAWHSK010000000
VERSION     DAWHSK010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.415"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
BASE COUNT          123 a          121 c           90 g          106 t
ORIGIN      
        1 caacgatgta ctggtttttg ttaatccact atatccgttt gcgcccgcca agccgtgttg
       61 cacagatgcg acacggcttt tttctttccg ttgtcagaat gtaaattttc gtcaaaaaac
      121 atcaaaaatc accacccccc cgaatttgcg taaaataaag tgtaatccct ttgattataa
      181 gggaatgttg acaattagat cgattctcct aggctgactt tcgcaataaa tggggttgtc
      241 aataattgtg tatggtcgtt tgccgtccgg cggccgtcat tcccgcgaac gcaggaatcc
      301 agcaacacaa tgcagcagga ctttatcgga aaaaaccgaa cccccaccgc cgtcattccc
      361 gcgcaggcgg gaatccaaag acgcaacgca acaggaattt atcggaaaaa acagaacccc
      421 cccaccgccg tcattcccgc
//
LOCUS       DAWHSK010000416          440 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.416, whole genome shotgun sequence.
ACCESSION   DAWHSK010000416 DAWHSK010000000
VERSION     DAWHSK010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.416"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..96
                     /locus_tag="WIC03_002187"
     CDS             <1..96
                     /locus_tag="WIC03_002187"
                     /inference="COORDINATES: protein motif:HMM:NF013842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEZ3884628.1"
                     /translation="CTAATVCYALKQMGITRKKRPPLTKNKTRPK"
     gene            105..>440
                     /locus_tag="WIC03_002188"
     CDS             105..>440
                     /locus_tag="WIC03_002188"
                     /inference="COORDINATES: protein motif:HMM:NF024751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ3884629.1"
                     /translation="MTQPAEFSDYQRVYLDETGFDRHLFRPYARSLKGQIVKAQISGK
                     RYRRLSLVSAQVGNRLIAPMVYQNTMTGVFFEAWFQQCLLPALTQKSVIILDNARFHR
                     MGVLREMAEK"
BASE COUNT          129 a          105 c          104 g          102 t
ORIGIN      
        1 tgtacggcag ccaccgtttg ctatgcactc aaacagatgg ggataacgcg caaaaaaaga
       61 ccaccactta caaagaacaa gacccggcca aagtaacgca ttatttgaca cagccggccg
      121 aattttctga ctaccaacgc gtttatttgg atgaaacagg atttgaccgc cacctgttcc
      181 gtccctatgc ccgcagcctg aaagggcaaa tagtgaaagc gcagataagt ggaaaaagat
      241 accgacgctt atctctggtg tccgcacaag tcggcaaccg gctgattgct ccgatggttt
      301 atcaaaatac gatgaccgga gtcttttttg aagcgtggtt tcagcaatgc ctactgcccg
      361 cattgactca aaaatcggtg attattttag ataatgcgcg atttcaccgt atgggtgtct
      421 tacgggaaat ggcggaaaaa
//
LOCUS       DAWHSK010000417          433 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.417, whole genome shotgun sequence.
ACCESSION   DAWHSK010000417 DAWHSK010000000
VERSION     DAWHSK010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.417"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            253..>433
                     /locus_tag="WIC03_002189"
     CDS             253..>433
                     /locus_tag="WIC03_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HEZ3884630.1"
                     /translation="MAAFNTQKVLSVHHWTDAYFTFTCTRDESLRFENGQFVMVGLMV
                     DGKPLMRAYSVASANW"
BASE COUNT          100 a          134 c           97 g          102 t
ORIGIN      
        1 gacgggacgc actcaggctg acagcctcga tttccctgat ttcccttgcc gatatgccgc
       61 cgtaagccgc cgcctgcccc gcgatgccgt ctgaagcctt tcagacggca tataaccaac
      121 gcgcatcaag cattgtcgca accatccgtt tcgtttagaa ttttggcttt atcccgaatc
      181 gggcagacat ttccactctg ccgcacctcc cgaacaagca ctatccattt cttttactga
      241 caggaaaccc aaatggcagc attcaatacc caaaaagtat tgtccgtaca ccactggaca
      301 gacgcatatt ttacctttac ctgcacccgc gacgaatcgt tgcgctttga aaacggacag
      361 ttcgttatgg tcggattgat ggtggacggc aagccgctga tgcgtgcata cagcgtcgcc
      421 tccgccaact ggg
//
LOCUS       DAWHSK010000418          429 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.418, whole genome shotgun sequence.
ACCESSION   DAWHSK010000418 DAWHSK010000000
VERSION     DAWHSK010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.418"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(1..265)
                     /locus_tag="WIC03_002190"
                     /pseudo
     CDS             complement(1..265)
                     /locus_tag="WIC03_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
BASE COUNT           90 a           91 c          110 g          138 t
ORIGIN      
        1 ccggctgtcc gcagaaccat tttaccgaac cgtcttgacg cttggcccac agggagagtt
       61 ttttgccttg gatttctttg tttacgccgc ttgatttcat ttcggcggta acgacgccgt
      121 ttttgacttc aacgctttta acatatttgc ctttgatgtc ggtggcggag gatgccacgc
      181 cggcagaagt gttgtttttc ggccattcgc cgtgattcag gtaatactcg gtgacggctg
      241 atttttgacc ttcagccaaa agaatgacga attttaggtt gctgtttttg gttttatgtt
      301 cttgtgggaa tgatgaaatt ttaagtttta ggaatttatc ggaaaaaaca gaaaccgctc
      361 cgccgtcatt cccgcgcagg cgggaatcta ggtctgtcgg tacggaaact tatcgggtaa
      421 aacggtttc
//
LOCUS       DAWHSK010000419          425 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.419, whole genome shotgun sequence.
ACCESSION   DAWHSK010000419 DAWHSK010000000
VERSION     DAWHSK010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.419"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
BASE COUNT           92 a           97 c          126 g          110 t
ORIGIN      
        1 gtgttgtccg gcaactgtcc ggttgtgtcg atgggttgcc ctgccgctcc ctgtgctgcg
       61 caacggttgt gtgccttggg tgggcgcgag tggcggccgg gcggtttgtt gctatgatac
      121 gggagttccg atatacaagg gtgggcttca gcccaccaat caacgaccga aaaagaaaaa
      181 acggtgggct gaagcccact ctacgcaatt acgcaactgt tatcgtgggg atgacgggat
      241 tttaggtttt tgtttttctg tttttgcggg aaagatgggt tttaggttgc ggtcatttat
      301 cggaaaagca gaaatcgctc cgccgtcatt cccgcgaaag cgggaatctg aacacgtccg
      361 tagggaaacc tatatcccgt cattcccacg aaagtgggaa tctagaacct ctgaattttc
      421 agaca
//
LOCUS       DAWHSK010000420          413 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.420, whole genome shotgun sequence.
ACCESSION   DAWHSK010000420 DAWHSK010000000
VERSION     DAWHSK010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.420"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(86..397)
                     /gene="rpsJ"
                     /locus_tag="WIC03_002191"
     CDS             complement(86..397)
                     /gene="rpsJ"
                     /locus_tag="WIC03_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEZ3884631.1"
                     /translation="MANQKIRIRLKAYDYALIDRSAQEIVETAKRTGAVVKGPIPLPT
                     KIERFNILRSPHVNKTSREQLEIRTHLRLMDIVDWTDKTTDALMKLDLPAGVDVEIKV
                     Q"
BASE COUNT          104 a           88 c           98 g          123 t
ORIGIN      
        1 ggattttggg gaattttgca aaggtctcgg cataacataa aaaatcccca agcattgatt
       61 gcttggggat tttcaatagt ccgaattatt ggactttgat ttctacatca acaccggccg
      121 gcaaatccag cttcatcagc gcatcggtag ttttatcggt ccaatccacg atgtccatca
      181 ggcgcaagtg ggtgcggatt tccaattgct cacgggaagt tttgttcacg tgcggagaac
      241 gcaaaatgtt gaaacgctcg attttggtcg gcaaaggaat cgggcctttt acaactgcac
      301 cggtacgttt tgcagtttca acgatttctt gtgcagaacg gtcaatcagg gcgtaatcat
      361 aagctttcag gcggatacgg attttttggt ttgccattta tcaatatcct tca
//
LOCUS       DAWHSK010000421          405 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.421, whole genome shotgun sequence.
ACCESSION   DAWHSK010000421 DAWHSK010000000
VERSION     DAWHSK010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.421"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..>405
                     /gene="mafA"
                     /locus_tag="WIC03_002192"
     CDS             <1..>405
                     /gene="mafA"
                     /locus_tag="WIC03_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HEZ3884632.1"
                     /translation="RSAGLSVNGTGDYRNETLLANPRDVSFLTNLIQTVFYLRGIEVV
                     PPEYADTDVFVTVDVFGTVRSRTELHLYNAETLKAQTKLEYFAVDRDSRKLLIAPKTA
                     AYESQYQEQYALWMGPYSVGKTVKASDRLMVD"
BASE COUNT          100 a          153 c           84 g           68 t
ORIGIN      
        1 acgctccgcc ggactgtccg tcaacggcac gggcgactac cgcaacgaaa ccctgctcgc
       61 caacccccgc gacgtttcct tcctgaccaa cctcatccaa accgtcttct acctgcgcgg
      121 catcgaagtc gtaccgcccg aatacgccga caccgacgta ttcgtaaccg tcgacgtatt
      181 cggcaccgtc cgcagccgca ccgaactgca cctctacaac gccgaaaccc ttaaagccca
      241 aaccaagctc gaatatttcg ccgttgaccg cgacagccgg aaactgctga ttgcccctaa
      301 aaccgccgcc tacgaatccc aataccaaga acaatacgcc ctctggatgg gaccttacag
      361 cgtcggcaaa accgtcaaag cctcagaccg cctgatggtc gattt
//
LOCUS       DAWHSK010000422          387 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.422, whole genome shotgun sequence.
ACCESSION   DAWHSK010000422 DAWHSK010000000
VERSION     DAWHSK010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.422"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..>387)
                     /locus_tag="WIC03_002193"
     CDS             complement(<1..>387)
                     /locus_tag="WIC03_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEZ3884633.1"
                     /translation="DHTHTHFDGKAQVAEQIKGIGSITTATLMAMLPELGRLSHKRIA
                     SLAGIAPHPRESGETKFKSRCFGGKSAVRKALYMATVAATRFEPLIRDFYQRLLSEGK
                     PYKVAVTACMRKLLTISNARMRDYFA"
BASE COUNT           79 a          116 c          101 g           91 t
ORIGIN      
        1 cggcaaaata atcacgcatc cgggcattcg atatcgtcag cagtttgcgc atacatgccg
       61 taacggcaac cttatacggc ttaccctcgg acagcaggcg ttggtagaaa tcccgaataa
      121 gcggttcaaa acgtgtcgct gccacggtag ccatatacag cgccttacgt accgcagact
      181 ttccgccaaa gcagcggctt ttgaatttgg tttccccgct ctccctcggg tgcggggcaa
      241 tgccggccaa actcgctatc cgtttgtgcg acagccgccc caattcgggc agcatcgcca
      301 tcagcgtagc cgtcgttatc gaaccgatgc ctttgatttg ctccgccact tgggctttgc
      361 cgtcaaaatg cgtgtgggtg tggtcgt
//
LOCUS       DAWHSK010000423          380 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.423, whole genome shotgun sequence.
ACCESSION   DAWHSK010000423 DAWHSK010000000
VERSION     DAWHSK010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.423"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            25..>380
                     /locus_tag="WIC03_002194"
     CDS             25..>380
                     /locus_tag="WIC03_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ3884634.1"
                     /translation="MDSRLRGNDESILLAEGQKSAVTEYYLNHGIWPANNNSAGVAST
                     ATDIKGKYVKEVTVANGVVTATMLSSGVNNEIQGKKLSLWAKRQNGSVKWFCGQPVTR
                     ANKATDDGVTADAAAK"
BASE COUNT          108 a          116 c           92 g           64 t
ORIGIN      
        1 cgataagttt ctgcaccgac aaggctggat tcccgcctgc gcgggaatga cgaatccatc
       61 cttttagccg aaggtcaaaa atcagccgtc accgagtatt acctgaatca cggcatatgg
      121 cccgccaaca acaattctgc cggcgtggca tccaccgcca ccgacatcaa aggcaaatat
      181 gttaaggaag ttacagtcgc aaacggcgtc gttaccgcca caatgctttc aagcggcgta
      241 aacaacgaaa tccaaggcaa aaaactctcc ctgtgggcca agcgtcaaaa cggttcggta
      301 aaatggttct gcggacagcc ggttacgcgc gccaacaaag ccaccgacga cggcgttacc
      361 gccgacgccg ccgccaagaa
//
LOCUS       DAWHSK010000424          375 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.424, whole genome shotgun sequence.
ACCESSION   DAWHSK010000424 DAWHSK010000000
VERSION     DAWHSK010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.424"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            92..>375
                     /locus_tag="WIC03_002195"
     CDS             92..>375
                     /locus_tag="WIC03_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma"
                     /protein_id="HEZ3884635.1"
                     /translation="MGHNMMTTQKWYEHITNVIIGNTANFNSGCPEAIDYVDERKGVP
                     LAAMRHILMYTEAAASHAYLFEHDLNKFKQYAYVAGKLGILRSVNSTDPEP"
BASE COUNT          125 a           70 c           76 g          104 t
ORIGIN      
        1 aaacggcaat agcaggcgtt gatcgtcaaa caggtaaggc cgttattctt tctgtcaaag
       61 ttccttctaa aaccaatata aggagataac aatggggcac aatatgatga ccacccaaaa
      121 atggtatgag catattacta atgtaatcat aggcaatact gctaatttca atagcggttg
      181 ccctgaagct atagattatg tagatgaaag aaaaggcgtt ccacttgcag caatgagaca
      241 tattttaatg tacactgaag ctgcggcttc ccatgcctat ctatttgaac atgatcttaa
      301 taaattcaag caatatgctt atgttgcagg aaagctgggt attttgcgga gtgtaaattc
      361 tacagaccct gaacc
//
LOCUS       DAWHSK010000425          373 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.425, whole genome shotgun sequence.
ACCESSION   DAWHSK010000425 DAWHSK010000000
VERSION     DAWHSK010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.425"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
BASE COUNT          105 a           84 c           79 g          105 t
ORIGIN      
        1 ttttttgaat ttcgggcaac gcttgaatct tcattccgcg caggcggaaa ttatcggtgc
       61 ggtacggcaa cttttttcga tatgaaaaga ccgtcattcc tgtaaaaaca aaaaatcaaa
      121 aacagaaaat tgaaattcgt cattcccgcg caggcgggaa tctaggacgt aaaatctata
      181 gtggattaac aaaaaccagt acagcgttgc cttgacttag ctcgaagaga acgattctct
      241 aaggtgctga agcaccgagt gagtcggttc cgcactatcc gtactgtctg cggcttcgtc
      301 gctttctcct gatttttgtt aatccactat aaagaaaccg tttttctcga taagtttccg
      361 tgccgacaga cct
//
LOCUS       DAWHSK010000426          371 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.426, whole genome shotgun sequence.
ACCESSION   DAWHSK010000426 DAWHSK010000000
VERSION     DAWHSK010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.426"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..354
                     /locus_tag="WIC03_002196"
     CDS             <1..354
                     /locus_tag="WIC03_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEZ3884636.1"
                     /translation="LVLNSLLGIFGEAYSEAMLMARQFGIDTDTIVEAIGGSAMDSPM
                     FQTKKSLWANREFPPAFALKHASKDLNLAVKELEQAGNTLPAVETVAASYRKAVEAGY
                     GEQDVSGVYLKLAEH"
BASE COUNT           90 a          125 c           89 g           67 t
ORIGIN      
        1 ctcgtcttga actcgctctt gggcattttc ggcgaagcgt acagcgaagc gatgctgatg
       61 gcgcggcagt tcggcatcga taccgacacc atcgtcgaag ccatcggcgg ctcggcaatg
      121 gactcgccca tgttccaaac caaaaaatcc ctgtgggcaa accgcgaatt cccacccgcc
      181 ttcgccctca aacacgcctc caaagacctc aacctcgccg tcaaagagct tgaacaggca
      241 ggcaacaccc tgcccgccgt cgaaaccgtt gctgccagct accgcaaagc agtcgaagcc
      301 ggctacggcg aacaggacgt ttccggcgtt tacctgaaat tggcagaaca ctgattgcct
      361 tttccaaaca c
//
LOCUS       DAWHSK010000427          370 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.427, whole genome shotgun sequence.
ACCESSION   DAWHSK010000427 DAWHSK010000000
VERSION     DAWHSK010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.427"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..312
                     /locus_tag="WIC03_002197"
     CDS             <1..312
                     /locus_tag="WIC03_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEZ3884637.1"
                     /translation="AVMIRHANGVETLYAHLSAFSQAQGNVRGGEVIGFVGSTGRSTG
                     PHLHYEARINGQPVNPVSVALPTPELTQADKAAFAAQKQKADALLARLRGIPVTVSQS
                     D"
BASE COUNT           80 a          104 c          117 g           69 t
ORIGIN      
        1 gcggtgatga tacgccacgc caacggtgtg gaaacgctgt acgcgcactt gagcgcgttt
       61 tcgcaggcac aaggcaatgt gcgcggcggc gaggtcatcg gttttgtcgg ttcgacaggg
      121 cgttcgaccg ggccgcacct gcattacgag gcacgcatca acgggcagcc cgtcaatcct
      181 gtttcagtcg cattgccgac acccgaattg acgcaggcgg acaaggcggc gtttgccgcg
      241 cagaaacaga aggcggacgc gctgcttgcg cgcttgcgcg gcataccggt taccgtgtcg
      301 caatcggatt gaagtttgaa ccggcgacga aaacaatgcc gtctgaaaat ctgcaaacag
      361 gttttcagac
//
LOCUS       DAWHSK010000428          344 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.428, whole genome shotgun sequence.
ACCESSION   DAWHSK010000428 DAWHSK010000000
VERSION     DAWHSK010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.428"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
BASE COUNT          112 a           86 c           94 g           52 t
ORIGIN      
        1 gaggcctgcg cgggaatgac gaaaagcaac ctttccgcgt cattcccgca aaagcgggaa
       61 tctagaaccc ttaacgcggc aggaatctat cggaaatgac tgaaaccgaa cgaacctaga
      121 ttcccgcctg tgcgggaatg acgaaaagca acctttccgc gtccttcccg cgaaagcggg
      181 aatctagaac ccctaacgtg gcaggaatct atcggaaaaa accgaaaccg aacagactgg
      241 attcccgcct gcgcgggaat gacgaagggt taaagaaatg acgagaaacg gcggtaattt
      301 attgggaata accgaaaacg aacggaaatc aggacaaggc ggcg
//
LOCUS       DAWHSK010000429          338 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.430, whole genome shotgun sequence.
ACCESSION   DAWHSK010000429 DAWHSK010000000
VERSION     DAWHSK010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.430"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..>338
                     /gene="mafB"
                     /locus_tag="WIC03_002198"
     CDS             <1..>338
                     /gene="mafB"
                     /locus_tag="WIC03_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HEZ3884638.1"
                     /translation="GKYHLFGDPRGSVSDRTGQINVIQDYTHRMGNLLIQQANINGTI
                     GYHTRFSGHGHEEHAPFDNHASRSASEEKGSVNEGFTVYRLNWEGHEHHPADAYDGPK
                     GGNYPKPTGAR"
BASE COUNT           94 a          121 c           80 g           43 t
ORIGIN      
        1 ggcaaatacc acctcttcgg cgacccgcgc ggcagcgttt ccgaccgcac cggtcaaatc
       61 aacgtcatcc aagactatac ccaccggatg ggcaacctgc tcatccagca ggcaaacatc
      121 aacggcacaa tcggctacca cacccgcttt tcaggacacg gacacgaaga acacgccccc
      181 tttgacaacc atgccagccg cagcgcaagc gaagaaaaag gcagcgtcaa cgaaggcttt
      241 accgtatacc gcctcaactg ggaagggcac gaacatcatc ccgccgatgc ctacgacggc
      301 ccgaagggcg gcaattaccc caaacctacg ggcgcacg
//
LOCUS       DAWHSK010000430          331 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.431, whole genome shotgun sequence.
ACCESSION   DAWHSK010000430 DAWHSK010000000
VERSION     DAWHSK010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.431"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            18..212
                     /gene="thiS"
                     /locus_tag="WIC03_002199"
     CDS             18..212
                     /gene="thiS"
                     /locus_tag="WIC03_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEZ3884639.1"
                     /translation="MNIILNGGPAELHGTTVADLIAQTAPQKPFAVAVNTVFVPKGAY
                     AETVLNENDKIDIVRPVVGG"
BASE COUNT           91 a           96 c           82 g           62 t
ORIGIN      
        1 agaaacatcg agaaaccatg aacatcatct taaacggcgg acccgccgaa cttcacggca
       61 cgaccgttgc cgacctcatc gcccaaaccg cgccgcaaaa gccctttgcc gtggcggtca
      121 acaccgtttt cgtccccaaa ggcgcgtatg cggaaacggt tttaaacgaa aacgacaaaa
      181 tcgatatcgt gcggccggtg gtcggcggct aggcggtttt gccttttcag acgacccctg
      241 tccccaaaac aacgttatgg tggattaact ttaaatcagg acaaggcgac gaagccgcag
      301 acagtacgga tagtacggaa ccgattcact t
//
LOCUS       DAWHSK010000431          321 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.432, whole genome shotgun sequence.
ACCESSION   DAWHSK010000431 DAWHSK010000000
VERSION     DAWHSK010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.432"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
BASE COUNT           78 a           77 c           86 g           80 t
ORIGIN      
        1 ggaatgacgt ggtgcaggtt tccgtgcgga tggcttcgtc attcccgcgc aggcgggaat
       61 ccagtccgct tgttcggtaa atgagagggc ggattgcgcg cctgtcagat aaaccccgtg
      121 tttaaacggg cggcaatgag gtacgcgcag agccttgaag cgcagtcgat atattatttt
      181 ccaccaaaac ggacgacccc gcctgccttg caaaccctta aaatgaagcc acccggatta
      241 atccgagtgg ccgtggaaaa tcacttaccg cttgatttat ttaaaattta tggtataatt
      301 tgccttaggc agtagtactt g
//
LOCUS       DAWHSK010000432          318 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.433, whole genome shotgun sequence.
ACCESSION   DAWHSK010000432 DAWHSK010000000
VERSION     DAWHSK010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.433"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..253
                     /locus_tag="WIC03_002200"
     CDS             <1..253
                     /locus_tag="WIC03_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ3884640.1"
                     /translation="DDAKDDAVTAAATGTDKIDTKHLPSTCRDASSASRTKTPGFAFK
                     YFQKISRYRVLPESRQMTKKLRHSRAGGNLGLSAQKLIG"
BASE COUNT           86 a           97 c           71 g           64 t
ORIGIN      
        1 agacgacgcc aaagacgacg ccgttaccgc cgccgccacc ggcaccgaca aaatcgacac
       61 caagcacctg ccgtcaacct gccgcgacgc ttcatctgcc agccgtacaa aaacacccgg
      121 tttcgctttc aaatacttcc aaaaaatcag ccgttaccga gtattgcccg aatcacggca
      181 aatgactaaa aaacttcgtc attcccgcgc aggcgggaat ctaggtttgt cggcgcagaa
      241 acttatcggg taaaaaggtt tctccggtcc tgagttctag attcccactt tcgtgggaat
      301 gacgggattc aatgatgc
//
LOCUS       DAWHSK010000433          315 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.434, whole genome shotgun sequence.
ACCESSION   DAWHSK010000433 DAWHSK010000000
VERSION     DAWHSK010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.434"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
BASE COUNT           94 a           56 c           77 g           88 t
ORIGIN      
        1 gtgggaatga cgggatttga ggtttctgtt tttgattttc tgttttcgtg ggaatgacgg
       61 tttagaagtt acccgaaacc tcaaaaaaac cgaaaccgaa cggatcggat taccgctttc
      121 gcgtttcaaa gttacggtgt tatcggaaaa acagaaaatc aaagctgcaa gaatttattt
      181 aaaacaaccg aatttcaacg gatcggattc tcgcctgtag ggaatgacgg cggaaggttt
      241 tttgtctttt ctgacagatg tccgcaatct gaaatcctga ccgtgggaac gacggtatag
      301 tggattaaca aaaac
//
LOCUS       DAWHSK010000434          301 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.435, whole genome shotgun sequence.
ACCESSION   DAWHSK010000434 DAWHSK010000000
VERSION     DAWHSK010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.435"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(63..>301)
                     /locus_tag="WIC03_002201"
     CDS             complement(63..>301)
                     /locus_tag="WIC03_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEZ3884641.1"
                     /translation="SNPPYGVRLEEVRALQALYPQLGTWLKKYYAGWLAAMFTGDREM
                     PKFMRLSPKRKIPLYNGNLDCRLFLIDMVQGSNR"
BASE COUNT           74 a           82 c           73 g           72 t
ORIGIN      
        1 tcggccggat attaaaaaaa gccgtctgaa catttttcag acggcatttt tgtacacttt
       61 cctcaacggt tcgatccctg caccatatca atcaggaaca ggcggcaatc gaggttgccg
      121 ttataaagcg gaattttgcg ttttggcgac aggcgcatga atttgggcat ttcccgatct
      181 ccggtaaaca ttgccgccaa ccagcctgcg taatatttct tcaaccacgt ccccaactgc
      241 ggataaagcg cctgcaaggc gcggacttcc tcaaggcgca cgccgtaggg cggattggac
      301 a
//
LOCUS       DAWHSK010000435          299 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.436, whole genome shotgun sequence.
ACCESSION   DAWHSK010000435 DAWHSK010000000
VERSION     DAWHSK010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.436"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..217)
                     /locus_tag="WIC03_002202"
     CDS             complement(<1..217)
                     /locus_tag="WIC03_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-regulated frpA domain protein"
                     /protein_id="HEZ3884642.1"
                     /translation="MTYANFNINGFGDVKLTPYSPLLGYKAWDSFIGSIQSLSDLIYN
                     VDNNRNKMEITVNNAIQAADSFLSSNWK"
BASE COUNT          116 a           45 c           35 g          103 t
ORIGIN      
        1 tcttccaatt actgcttaaa aagctatctg cagcttgaat agcattatta acagtaattt
       61 ccattttatt tctattgtta tccacattat agattaaatc agataaggat tgaatagaac
      121 caataaatga atcccaagct ttatatccca agagtggtga atagggtgtt aatttcacat
      181 ctccaaaacc gttaatatta aaatttgcat atgtcatatc tgctttcctt tcaagtaaat
      241 taaaaaaata acgtgaggaa ttaaaattaa aaataaatta attatcgact caatataat
//
LOCUS       DAWHSK010000436          290 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.437, whole genome shotgun sequence.
ACCESSION   DAWHSK010000436 DAWHSK010000000
VERSION     DAWHSK010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..290
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.437"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
BASE COUNT           80 a           79 c           72 g           59 t
ORIGIN      
        1 aatattgccg ccgtcccgcg ttctagattc ccgcctgcgc gggaatgacg attcatcagt
       61 ttcccgaaac gcccgaaaaa ccgaaaccga gcaaaccgga ttcccgcctg cgcgggaatg
      121 acgatttata agtttcccga aacgcccaaa aaaaccgaaa tccgacaggc aggatttttg
      181 ctttcgtggg gatgacggga ttttaagtta cggtcattta tcggaaaagc agaaactgct
      241 ccgccgttat tccagcgaag gcgggaatct gaacacgtcc gcacggaaac
//
LOCUS       DAWHSK010000437          289 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.438, whole genome shotgun sequence.
ACCESSION   DAWHSK010000437 DAWHSK010000000
VERSION     DAWHSK010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.438"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..>289
                     /locus_tag="WIC03_002203"
     CDS             <1..>289
                     /locus_tag="WIC03_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ3884643.1"
                     /translation="CRYRNWLAQDDTLSELLELINRQLTEKGLKIEKASAAVVDATII
                     QTAGSKQRQAIEVDEEGQVSGQTTPSKDSDARWTKKNGLYKLGYKQHTRTDA"
BASE COUNT           94 a           85 c           68 g           42 t
ORIGIN      
        1 tatgccgtta ccgcaactgg ctggcgcaag acgacaccct gtccgaattg ctggaactga
       61 ttaaccgcca actgaccgaa aaaggcttaa aaatagagaa agcatccgcc gccgtcgttg
      121 acgccaccat tattcagacc gccggcagca aacagcgcca ggccatagaa gttgacgaag
      181 aaggacaagt cagcggccaa accacaccga gtaaagacag cgatgcccgt tggacaaaga
      241 aaaacggcct ctacaaactc ggttacaaac agcatacccg taccgatgc
//
LOCUS       DAWHSK010000438          283 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.439, whole genome shotgun sequence.
ACCESSION   DAWHSK010000438 DAWHSK010000000
VERSION     DAWHSK010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..283
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.439"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..>283
                     /locus_tag="WIC03_002204"
     CDS             <1..>283
                     /locus_tag="WIC03_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ3884644.1"
                     /translation="RDASSAVCTKTPEYYLNHGEWPGNNTSAGVASSTDIKGKYVQSV
                     EVKNGVVTATMLSTGVNNEIKGKKLSLWAKRQAGSVKWFCGQPVTRAANA"
BASE COUNT           82 a           86 c           66 g           49 t
ORIGIN      
        1 cgcgacgcat catctgccgt ttgcacaaaa acacccgagt attacctgaa tcacggcgaa
       61 tggcccggca acaacacttc tgccggcgtg gcatcctcaa ccgacatcaa aggcaaatat
      121 gttcaaagcg ttgaagtcaa aaacggcgtc gttaccgcca caatgctttc aaccggcgta
      181 aacaacgaaa tcaaaggcaa aaaactctcc ctgtgggcca agcgtcaagc cggttcggta
      241 aaatggttct gcggacagcc ggttacgcgc gccgccaacg cca
//
LOCUS       DAWHSK010000439          278 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.440, whole genome shotgun sequence.
ACCESSION   DAWHSK010000439 DAWHSK010000000
VERSION     DAWHSK010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.440"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..243
                     /locus_tag="WIC03_002205"
     CDS             <1..243
                     /locus_tag="WIC03_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ3884645.1"
                     /translation="SLLRLGAAKRHYEKPGFFSGFKGERRRDKELNTSLSLWHRALHF
                     KGITPRLTLSHRETRSNDVFNEYEKNRAFVEFNKTF"
BASE COUNT           69 a           66 c           79 g           64 t
ORIGIN      
        1 tcgctgttgc gcctcggcgc ggcgaaacgg cattatgaaa aacccggctt tttcagcggt
       61 tttaaagggg aaaggcgcag ggataaagaa ttgaacacat ccttgagcct ttggcaccgg
      121 gcattgcatt tcaaaggcat cacgccgcgc ctgacgttgt cgcaccgcga aacgcggagt
      181 aacgatgtgt tcaacgaata cgagaaaaat cgggcgtttg tcgagtttaa taaaacgttc
      241 tgattgctgt tccttttcgg aggaaaccct gccggcgg
//
LOCUS       DAWHSK010000440          276 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.441, whole genome shotgun sequence.
ACCESSION   DAWHSK010000440 DAWHSK010000000
VERSION     DAWHSK010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..276
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.441"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
BASE COUNT          103 a           28 c           52 g           93 t
ORIGIN      
        1 ttagcagaag gatatactta tttcttcaat gaaaatacgg atgattgcta tgttttgaaa
       61 gaggatttta ttaataatga gcgatatgaa aaaacagaat tatattccca aaaagataag
      121 gtaattctat ttccaaagtt tggtgaatat gatttggtgt taaatccgga cattatttaa
      181 ttaagtttta aggccgtctg aaaaaaattt caaacggctt ttattattgg gtttggaatc
      241 tgaggataaa gctgataaaa accaggaaat tatcag
//
LOCUS       DAWHSK010000441          274 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.442, whole genome shotgun sequence.
ACCESSION   DAWHSK010000441 DAWHSK010000000
VERSION     DAWHSK010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.442"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
BASE COUNT           66 a           73 c           67 g           68 t
ORIGIN      
        1 cttatcgggc aaaacggttt cttgagattc cgcatcctag attcccgcct gcgcgggaat
       61 gacggcgcat aagtttctgt gttgatttga gtctatggga acggtgtagg attggtggaa
      121 tcgttggaat cggtgggctg aagcccaccc tataactcac cttttacgaa tcctcctaca
      181 ccctatacaa caccttgaaa atccaccttg tcgggaatgc ccgaaccgtc agaaataccc
      241 gaatcgtcat tcccgcgcag gcgggaatct agac
//
LOCUS       DAWHSK010000442          271 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.443, whole genome shotgun sequence.
ACCESSION   DAWHSK010000442 DAWHSK010000000
VERSION     DAWHSK010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..271
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.443"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
BASE COUNT           85 a           83 c           44 g           59 t
ORIGIN      
        1 aacggcaatc catacgatac agatcatagc aacagccatc gcaacaacgt tggcaaaatc
       61 aggggactcc gacattggcg catagcatct gccgatgcac ggctcctcat tcggctctat
      121 gaataccata cccatcacaa aatccaccgc caaaaccagg cacggcttct tatacttatg
      181 atagatttcc accatcctgt cccatatata ccaaacattc ataccgtata tcccgcaggc
      241 aacaaattcc gattgaaggt tacagcccta t
//
LOCUS       DAWHSK010000443          268 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.444, whole genome shotgun sequence.
ACCESSION   DAWHSK010000443 DAWHSK010000000
VERSION     DAWHSK010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..268
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.444"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
BASE COUNT           57 a           67 c           85 g           59 t
ORIGIN      
        1 gttgccgcct aaaaggcagc acggatgcct gattatcggg tatccgggga ggattaaggg
       61 ggcgtttggg tagaattagg agatatttgg ggcgaaaaca gccgaaaacc tgtgttgggg
      121 tttcggctgt cgggagggaa aggaattttg caaaggtctc aagctatgcc ggctgctgcc
      181 gcccacttca tctgcgccaa atcctgccgc ccaccgccga agccctcatg cgttcgcggt
      241 acagcgcgta tgtgctgcac cttatcga
//
LOCUS       DAWHSK010000444          268 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.445, whole genome shotgun sequence.
ACCESSION   DAWHSK010000444 DAWHSK010000000
VERSION     DAWHSK010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..268
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.445"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
BASE COUNT           45 a           51 c           85 g           87 t
ORIGIN      
        1 gtttctgttt ttggttttct gtccttgtgg gaatgatgaa aatttaagtt ttaggaattt
       61 atcggaaaaa acagaaaccg ctccgctgtc attcccgcgc aggcgggaat ccagccccgt
      121 cggtacggaa acttatcggg taaaaaggtt tctctagttt ggtgtcgatt ttcttgtcga
      181 tgctgttgac ggcaggtgct tggtgtcgat ctgcttgccg ttggcggcgg tgtcggcttt
      241 gacgtcgtcg ttggtggtgg tgggggcg
//
LOCUS       DAWHSK010000445          266 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.446, whole genome shotgun sequence.
ACCESSION   DAWHSK010000445 DAWHSK010000000
VERSION     DAWHSK010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..266
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.446"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            31..201
                     /locus_tag="WIC03_002206"
     CDS             31..201
                     /locus_tag="WIC03_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HEZ3884646.1"
                     /translation="MAQYMCGPCGWIYDEELGDPEHGIPPGTKFEDIPDDWKCPECGV
                     GKEDFYLLDFVI"
BASE COUNT           73 a           75 c           71 g           47 t
ORIGIN      
        1 atccgctata caaaaaacaa ggaaacacaa atggcgcaat atatgtgcgg cccctgcggc
       61 tggatttacg atgaagaact cggcgaccca gaacacggca tcccccctgg gacaaagttt
      121 gaagacattc ccgacgactg gaaatgcccc gaatgcggcg taggtaaaga agatttctac
      181 ctgttggatt tcgtgatata gcggcagccg cacccttagg gaaaaccccg tcatccggcg
      241 ttccgcctgc ggcggcaggg aacgcc
//
LOCUS       DAWHSK010000446          260 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.447, whole genome shotgun sequence.
ACCESSION   DAWHSK010000446 DAWHSK010000000
VERSION     DAWHSK010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..260
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.447"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            complement(<1..245)
                     /locus_tag="WIC03_002207"
     CDS             complement(<1..245)
                     /locus_tag="WIC03_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S ferredoxin-like protein"
                     /protein_id="HEZ3884647.1"
                     /translation="MARIGTNKGFFELLEGETLLEGLERTGHMVEYQCRSGYCGSCRV
                     KILEGSVTYREPPLAFLGRDEILPCCCCVEGDVRLDCG"
BASE COUNT           69 a          100 c           43 g           48 t
ORIGIN      
        1 ccgcaatcca gcctgacatc cccctcgacg caacagcagc acggcagtat ctcgtcccgc
       61 cccaaaaaag ccaaaggcgg ctcccgataa gtaacgcttc cctccaaaat cttcactcgg
      121 cacgatccgc aatatccgct tcggcactga tattccacca tatgccccgt ccgttccaag
      181 ccttccaaca gggtctcgcc ctccaagagc tcaaaaaaac ccttattcgt accaatgcgc
      241 gccatttccg accaatcaaa
//
LOCUS       DAWHSK010000447          258 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.448, whole genome shotgun sequence.
ACCESSION   DAWHSK010000447 DAWHSK010000000
VERSION     DAWHSK010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..258
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.448"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..>258
                     /locus_tag="WIC03_002208"
     CDS             <1..>258
                     /locus_tag="WIC03_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEZ3884648.1"
                     /translation="YATGRKDIIRFTDGITADMLTFTREGNHLLIKAKDGSGQVTVQS
                     YFQNDGSGAYRIDEIHFDNGKVLDVATVKKLVQQSTDGSDR"
BASE COUNT           74 a           65 c           59 g           60 t
ORIGIN      
        1 actacgctac cggacgcaaa gacatcatcc gctttaccga cggtattaca gccgatatgc
       61 tgacttttac ccgagagggc aaccatcttc ttatcaaggc aaaagacggc agtggacaag
      121 tgactgttca gtcctatttc cagaacgatg gctcaggtgc ttaccgtatc gatgagattc
      181 atttcgataa cggcaaagta ctggatgttg ccactgtcaa aaaactggta cagcaatcca
      241 ccgacggttc ggacagat
//
LOCUS       DAWHSK010000448          253 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.449, whole genome shotgun sequence.
ACCESSION   DAWHSK010000448 DAWHSK010000000
VERSION     DAWHSK010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.449"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..>253
                     /locus_tag="WIC03_002209"
     CDS             <1..>253
                     /locus_tag="WIC03_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ3884649.1"
                     /translation="SVPTRLDSRLRGNDEAILLAEGQKSAVTEYYLNHGIWPANNSDA
                     GVASSASDIKGKYVQSVIVANGVVTAEMKSTGVNKEIKGK"
BASE COUNT           77 a           69 c           60 g           47 t
ORIGIN      
        1 ttccgtaccg acaaggctgg attcccgcct gcgcgggaat gacgaagcca ttcttttggc
       61 tgaaggtcaa aaatcagccg tcacagagta ttacctgaat cacggcatat ggcccgccaa
      121 caacagtgat gccggcgtgg catcctccgc ctccgacatc aaaggcaaat atgttcaaag
      181 cgttatagtc gcaaacggcg tcgttaccgc cgaaatgaaa tcaaccggcg taaacaaaga
      241 aatcaaaggc aaa
//
LOCUS       DAWHSK010000449          251 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.450, whole genome shotgun sequence.
ACCESSION   DAWHSK010000449 DAWHSK010000000
VERSION     DAWHSK010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..251
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.450"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
BASE COUNT           86 a           65 c           36 g           64 t
ORIGIN      
        1 cggaaaccta tatcccgtca ttcttacgaa cctacatccc gtcattccct caaaaacaga
       61 aaaccaaaat tagaaatcta aaatcccgtc attcccacga aagtgggaat ctagttcgtt
      121 gagtttcagt tatttagaat aaattttgaa actttaatcc cgtcattccc gcaaaagcgg
      181 gaatccagaa cgtaaaatct aaagaaaccg ttttacccga taagtttccg caccgacaga
      241 cctagattcc c
//
LOCUS       DAWHSK010000450          250 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.451, whole genome shotgun sequence.
ACCESSION   DAWHSK010000450 DAWHSK010000000
VERSION     DAWHSK010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..250
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.451"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
BASE COUNT           74 a           54 c           50 g           72 t
ORIGIN      
        1 aaatgtcttg gtgggaattt aggggatttt ggggaatttt gcaaaggtct caaaccgttt
       61 ttctcgataa gtttccgtgc cgacagacct agattcccac tttcgtggga atgacggtgg
      121 aaaagttgcc gtgatttcgg ataaattttc gtaacgcata atttccgttt tacccgataa
      181 atgcccgcaa tctcaaatcc cgtcattccc caaaaacaaa aaatcaaaaa cagaaatatc
      241 gtcattcccg
//
LOCUS       DAWHSK010000451          241 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.452, whole genome shotgun sequence.
ACCESSION   DAWHSK010000451 DAWHSK010000000
VERSION     DAWHSK010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..241
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.452"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..>241
                     /locus_tag="WIC03_002210"
     CDS             <1..>241
                     /locus_tag="WIC03_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ3884650.1"
                     /translation="VDKNSDTMHFKGFKAADVHFIRSGSDLVLSASEQDNVRISGFFY
                     GENHRVDTFVFDDAAISNPDFAKYINAGNNLVQSMS"
BASE COUNT           69 a           42 c           49 g           81 t
ORIGIN      
        1 atgtggataa aaactctgac actatgcact ttaaaggatt taaagcagca gatgttcatt
       61 ttatccgttc cggaagtgat ttggtgctta gcgcttctga acaagacaac gtacgtattt
      121 ccggattctt ctatggtgaa aaccatcgtg tagatacatt tgtctttgat gatgcagcta
      181 tcagtaatcc agattttgcc aagtatatta atgctggcaa taatttggta cagtctatgt
      241 c
//
LOCUS       DAWHSK010000452          230 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.453, whole genome shotgun sequence.
ACCESSION   DAWHSK010000452 DAWHSK010000000
VERSION     DAWHSK010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..230
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.453"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
BASE COUNT           54 a           64 c           53 g           59 t
ORIGIN      
        1 taaatcctcg caatccaaag cctcgtcatt tccacaaaaa acagcaaccc gaaacacccc
       61 gtcattcccg cgcaggcggg aatctaggtc tttaaacttc ggttttttcc gataaattct
      121 tgccgtattg aaattctaga ttcccgcttt ctcgggaatg acgggatatg ggtttccgtg
      181 cggacgtgtt cggatttccg cctgcgcggg aatgacggcg acagatgccc
//
LOCUS       DAWHSK010000453          229 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.454, whole genome shotgun sequence.
ACCESSION   DAWHSK010000453 DAWHSK010000000
VERSION     DAWHSK010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..229
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.454"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
BASE COUNT           60 a           78 c           44 g           47 t
ORIGIN      
        1 acccgtcatt cccgataaca gcgcaatctt gaaacccgtc attcccgaca acaccgtaat
       61 ctcgaaattc gtcattcccg cgcaggcggg aatccagacc tgtccgcaca gaaacttatc
      121 ggataaaaac agttgcccaa accccgcgtt ctagattccc acttccgtgg gaatgacggt
      181 tcagttgcgt tccgacaaca ccgcaatctc gaaacccgtc attcccgcg
//
LOCUS       DAWHSK010000454          227 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.455, whole genome shotgun sequence.
ACCESSION   DAWHSK010000454 DAWHSK010000000
VERSION     DAWHSK010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..227
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.455"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
BASE COUNT           56 a           54 c           73 g           44 t
ORIGIN      
        1 gccgacaaac cgatgccgtc attcccgcgc aggcgggaat ctaggacgta aaatctcaag
       61 aaaccgttgt acccgataag tttccgtgcc gacaagtccg gattcccgcc tgcgcgggaa
      121 tgacggttca gttgcgtagg actggattgt gaaaaggggc ggattcggtg aaaacggcgg
      181 aaatgtggga ttgatggaat cggtgggctg aagccctccc tacagag
//
LOCUS       DAWHSK010000455          225 bp    DNA     linear   BCT 19-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain NM03769
            SAMN17286446-rid13893043.denovo.456, whole genome shotgun sequence.
ACCESSION   DAWHSK010000455 DAWHSK010000000
VERSION     DAWHSK010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17286446
            Sequence Read Archive: SRR13407343
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 08-FEB-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001243122.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001243122.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/18/2022 19:02:01
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,211
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 88
            CDSs (without protein)            :: 88
            Pseudo Genes (ambiguous residues) :: 0 of 88
            Pseudo Genes (frameshifted)       :: 45 of 88
            Pseudo Genes (incomplete)         :: 42 of 88
            Pseudo Genes (internal stop)      :: 11 of 88
            Pseudo Genes (multiple problems)  :: 10 of 88
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..225
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17286446-rid13893043.denovo.456"
                     /strain="NM03769"
                     /isolation_source="Oropharyngeal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="USA: Georgia"
                     /lat_lon="32.1656 N 82.9001 W"
                     /collection_date="2008"
                     /collected_by="Georgia Active Bacterial Core surveillance"
     gene            <1..>225
                     /locus_tag="WIC03_002211"
     CDS             <1..>225
                     /locus_tag="WIC03_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FrpC operon protein"
                     /protein_id="HEZ3884651.1"
                     /translation="IPENPYFHEFYYIKKGENLAIITHRNYHRYGENDYSTSVGSCIN
                     GFTVQYYPFIREKQQLTQQELVGYHQQVEQL"
BASE COUNT           75 a           40 c           47 g           63 t
ORIGIN      
        1 attcctgaaa atccatattt tcatgaattt tactatatta aaaaaggaga aaatctggcg
       61 attattactc atcggaacta tcataggtat ggagagaacg attacagcac tagcgtaggt
      121 tcctgtatta acggtttcac ggtacagtat tacccgttta ttcgggaaaa gcagcagctc
      181 acacagcagg agttggtagg ttatcaccaa caagtagagc aattg
//
